Methods For Synthesis Of Encoded Libraries

Abstract

The present invention provides a method of synthesizing libraries of molecules which include an encoding oligonucleotide tag.

Description

RELATED APPLICATIONS

[0001] This application is a continuation application of U.S. patent application Ser. No. 11/906,939 filed Oct. 4, 2007, which is a divisional of U.S. patent application Ser. No. 11/015,458 filed Dec. 17, 2004, which claims priority to U.S. Provisional Patent Application Ser. No. 60/530,854, filed on Dec. 17, 2003; U.S. Provisional Patent Application Ser. No. 60/540,681, filed on Jan. 30, 2004; U.S. Provisional Patent Application Ser. No. 60/553,715 filed Mar. 15, 2004; and U.S. Provisional Patent Application Ser. No. 60/588,672 filed Jul. 16, 2004. The entire contents of each of the foregoing applications are incorporated herein by reference.

SEQUENCE LISTING

[0002] The instant application contains a Sequence Listing which has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Feb. 8, 2011, is named SequenceListing.txt, and is 308,650 bytes in size.

BACKGROUND OF THE INVENTION

[0003] The search for more efficient methods of identifying compounds having useful biological activities has led to the development of methods for screening vast numbers of distinct compounds, present in collections referred to as combinatorial libraries. Such libraries can include 10.sup.5 or more distinct compounds. A variety of methods exist for producing combinatorial libraries, and combinatorial syntheses of peptides, peptidomimetics and small organic molecules have been reported.

[0004] The two major challenges in the use of combinatorial approaches in drug discovery are the synthesis of libraries of sufficient complexity and the identification of molecules which are active in the screens used. It is generally acknowledged that greater the degree of complexity of a library, i.e., the number of distinct structures present in the library, the greater the probability that the library contains molecules with the activity of interest. Therefore, the chemistry employed in library synthesis must be capable of producing vast numbers of compounds within a reasonable time frame. However, for a given formal or overall concentration, increasing the number of distinct members within the library lowers the concentration of any particular library member. This complicates the identification of active molecules from high complexity libraries.

[0005] One approach to overcoming these obstacles has been the development of encoded libraries, and particularly libraries in which each compound includes an amplifiable tag. Such libraries include DNA-encoded libraries, in which a DNA tag identifying a library member can be amplified using techniques of molecular biology, such as the polymerase chain reaction. However, the use of such methods for producing very large libraries is yet to be demonstrated, and it is clear that improved methods for producing such libraries are required for the realization of the potential of this approach to drug discovery.

SUMMARY OF THE INVENTION

[0006] The present invention provides a method of synthesizing libraries of molecules which include an encoding oligonucleotide tag. The method utilizes a "split and pool" strategy in which a solution comprising an initiator, comprising a first building block linked to an encoding oligonucleotide, is divided ("split") into multiple fractions. In each fraction, the initiator is reacted with a second, unique, building block and a second, unique oligonucleotide which identifies the second building block. These reactions can be simultaneous or sequential and, if sequential, either reaction can precede the other. The dimeric molecules produced in each of the fractions are combined ("pooled") and then divided again into multiple fractions. Each of these fractions is then reacted with a third unique (fraction-specific) building block and a third unique oligonucleotide which encodes the building block. The number of unique molecules present in the product library is a function of (1) the number of different building blocks used at each step of the synthesis, and (2) the number of times the pooling and dividing process is repeated.

[0007] In one embodiment, the invention provides a method of synthesizing a molecule comprising or consisting of a functional moiety which is operatively linked to an encoding oligonucleotide. The method includes the steps of: (1) providing an initiator compound consisting of a functional moiety comprising n building blocks, where n is an integer of 1 or greater, wherein the functional moiety comprises at least one reactive group and wherein the functional moiety is operatively linked to an initial oligonucleotide; (2) reacting the initiator compound with a building block comprising at least one complementary reactive group, wherein the at least one complementary reactive group is complementary to the reactive group of step (1), under suitable conditions for reaction of the reactive group and the complementary reactive group to form a covalent bond; (3) reacting the initial oligonucleotide with an incoming oligonucleotide which identifies the building block of step (b) in the presence of an enzyme which catalyzes ligation of the initial oligonucleotide and the incoming oligonucleotide, under conditions suitable for ligation of the incoming oligonucleotide and the initial oligonucleotide, thereby producing a molecule which comprises or consists of a functional moiety comprising n+1 building blocks which is operatively linked to an encoding oligonucleotide. If the functional moiety of step (3) comprises a reactive group, steps 1-3 can repeated one or more times, thereby forming cycles 1 to i, where i is an integer of 2 or greater, with the product of step (3) of a cycle s, where s is an integer of i-1 or less, becoming the initiator compound of cycle s+1.

[0008] In one embodiment, the invention provides a method of synthesizing a library of compounds, wherein the compounds comprise a functional moiety comprising two or more building blocks which is operatively linked to an oligonucleotide which identifies the structure of the functional moiety. The method comprises the steps of (1) providing a solution comprising m initiator compounds, wherein m is an integer of 1 or greater, where the initiator compounds consist of a functional moiety comprising n building blocks, where n is an integer of 1 or greater, which is operatively linked to an initial oligonucleotide which identifies the n building blocks; (2) dividing the solution of step (1) into r fractions, wherein r is an integer of 2 or greater; (3) reacting the initiator compounds in each fraction with one of r building blocks, thereby producing r fractions comprising compounds consisting of a functional moiety comprising n+1 building blocks operatively linked to the initial oligonucleotide; (4) reacting the initial oligonucleotide in each fraction with one of a set of r distinct incoming oligonucleotides in the presence of an enzyme which catalyzes the ligation of the incoming oligonucleotide and the initial oligonucleotide, under conditions suitable for enzymatic ligation of the incoming oligonucleotide and the initial oligonucleotide, thereby producing r aliquots comprising molecules consisting of a functional moiety comprising n+1 building blocks operatively linked to an elongated oligonucleotide which encodes the n+1 building blocks. Optionally, the method can further include the step of (5) recombining the r fractions produced in step (4), thereby producing a solution comprising compounds consisting of a functional moiety comprising n+1 building blocks, which is operatively linked to an elongated oligonucleotide. Steps (1) to (5) can be conducted one or more times to yield cycles 1 to i, where i is an integer of 2 or greater. In cycle s+1, where s is an integer of i-1 or less, the solution comprising m initiator compounds of step (1) is the solution of step (5) of cycle s. Likewise, the initiator compounds of step (1) of cycle s+1 are the compounds of step (5) of cycle s.

[0009] In a preferred embodiment, the building blocks are coupled in each step using conventional chemical reactions. The building blocks can be coupled to produce linear or branched polymers or oligomers, such as peptides, peptidomimetics, and peptoids, or non-oligomeric molecules, such as molecules comprising a scaffold structure to which is attached one or more additional chemical moieties. For example, if the building blocks are amino acid residues, the building blocks can be coupled using standard peptide synthesis strategies, such as solution-phase or solid phase synthesis using suitable protection/deprotection strategies as are known in the field. Preferably, the building blocks are coupled using solution phase chemistry. The encoding oligonucleotides are single stranded or double stranded oligonucleotides, preferably double-stranded oligonucleotides. The encoding oligonucleotides are preferably oligonucleotides of 4 to 12 bases or base pairs per building block; the encoding oligonucleotides can be coupled using standard solution phase or solid phase oligonucleotide synthetic methodology, but are preferably coupled using a solution phase enzymatic process. For example, the oligonucleotides can be coupled using a topoisomerase, a ligase, or a DNA polymerase, if the sequence of the encoding oligonucleotides includes an initiation sequence for ligation by one of these enzymes. Enzymatic coupling of the encoding oligonucleotides offers the advantages of (1) greater accuracy of addition compared to standard synthetic (non-enzymatic) coupling; and (2) the use of a simpler protection/deprotection strategy.

[0010] In another aspect, the invention provides compounds of Formula I:

##STR00001##

where X is a functional moiety comprising one or more building blocks; Z is an oligonucleotide attached at its 3' terminus to B; Y is an oligonucleotide which is attached at its 5' terminus to C; A is a functional group that forms a covalent bond with X; B is a functional group that forms a bond with the 3'-end of Z; C is a functional group that forms a bond with the 5'-end of Y; D, F and E are each, independently, a bifunctional linking group; and S an atom or a molecular scaffold. Such compounds include those which are synthesized using the methods of the invention.

[0011] The invention further relates to a compound library comprising compounds comprising a functional moiety comprising two or more building blocks which is operatively linked to an oligonucleotide which encodes the structure of the functional moiety. Such libraries can comprise from about 10.sup.2 to about 10.sup.12 or more distinct members, for example, 10.sup.2, 10.sup.3, 10.sup.4, 10.sup.5, 10.sup.6, 10.sup.7, 10.sup.8, 10.sup.9, 10.sup.10, 10.sup.11, 10.sup.12 or more distinct members, i.e., distinct molecular structures.

[0012] In one embodiment, the compound library comprises compounds which are each independently of Formula I:

##STR00002##

where X is a functional moiety comprising one or more building blocks; Z is an oligonucleotide attached at its 3' terminus to B; Y is an oligonucleotide which is attached at its 5' terminus to C; A is a functional group that forms a covalent bond with X; B is a functional group that forms a bond with the 3'-end of Z; C is a functional group that forms a bond with the 5'-end of Y; D, F and E are each, independently, a bifunctional linking group; and S an atom or a molecular scaffold. Such libraries include those which are synthesized using the methods of the invention.

[0013] In another aspect, the invention provides a method for identifying a compound which binds to a biological target, said method comprising the steps of: (a) contacting the biological target with a compound library of the invention, where the compound library includes compounds which comprise a functional moiety comprising two or more building blocks which is operatively linked to an oligonucleotide which encodes the structure of the functional moiety. This step is conducted under conditions suitable for at least one member of the compound library to bind to the target; (2) removing library members that do not bind to the target; (3) amplifying the encoding oligonucleotides of the at least one member of the compound library which binds to the target; (4) sequencing the encoding oligonucleotides of step (3); and using the sequences determined in step (5) to determine the structure of the functional moieties of the members of the compound library which bind to the biological target.

[0014] The present invention provides several advantages in the identification of molecules having a desired property. For example, the methods of the invention allow the use of a range of chemical reactions for constructing the molecules in the presence of the oligonucleotide tag. The methods of the invention also provide a high-fidelity means of incorporating oligonucleotide tags into the chemical structures so produced. Further, they enable the synthesis of libraries having a large number of copies of each member, thereby allowing multiple rounds of selection against a biological target while leaving a sufficient number of molecules following the final round for amplification and sequence of the oligonucleotide tags.

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] FIG. 1 is a schematic representation of ligation of double stranded oligonucleotides, in which the initial oligonucleotide has an overhang which is complementary to the overhang of the incoming oligonucleotide. The initial strand is represented as either free, conjugated to an aminohexyl linker or conjugated to a phenylalanine residue via an aminohexyl linker. FIG. 1 discloses SEQ ID NOs:896-901, respectively, in order of appearance.

[0016] FIG. 2 is a schematic representation of oligonucleotide ligation using a splint strand. In this embodiment, the splint is a 12-mer oligonucleotide with sequences complementary to the single-stranded initial oligonucleotide and the single-stranded incoming oligonucleotide.

[0017] FIG. 3 is a schematic representation of ligation of an initial oligonucleotide and an incoming oligonucleotide, when the initial oligonucleotide is double-stranded with covalently linked strands, and the incoming oligonucleotide is double-stranded.

[0018] FIG. 4 is a schematic representation of oligonucleotide elongation using a polymerase. The initial strand is represented as either free, conjugated to an aminohexyl linker or conjugated to a phenylalanine residue via an aminohexyl linker. FIG. 4 discloses SEQ ID NOs:896, 902, 903, and 904, respectively, in order of appearance.

[0019] FIG. 5 is a schematic representation of the synthesis cycle of one embodiment of the invention.

[0020] FIG. 6 is a schematic representation of a multiple round selection process using the libraries of the invention.

[0021] FIG. 7 is a gel resulting from electrophoresis of the products of each of cycles 1 to 5 described in Example 1 and following ligation of the closing primer. Molecular weight standards are shown in lane 1, and the indicated quantities of a hyperladder, for DNA quantitation, are shown in lanes 9 to 12.

[0022] FIG. 8 is a schematic depiction of the coupling of building blocks using azide-alkyne cycloaddition.

[0023] FIGS. 9 and 10 illustrate the coupling of building blocks via nucleophilic aromatic substitution on a chlorinated triazine.

[0024] FIG. 11 shows representative chlorinated heteroaromatic structures suitable for use in the synthesis of functional moieties.

[0025] FIG. 12 illustrates the cyclization of a linear peptide using the azide/alkyne cycloaddition reaction.

[0026] FIG. 13a is a chromatogram of the library produced as described in Example 2 following Cycle 4.

[0027] FIG. 13b is a mass spectrum of the library produced as described in Example 2 following Cycle 4.

DETAILED DESCRIPTION OF THE INVENTION

[0028] The present invention relates to methods of producing compounds and combinatorial compound libraries, the compounds and libraries produced via the methods of the invention, and methods of using the libraries to identify compounds having a desired property, such as a desired biological activity. The invention further relates to the compounds identified using these methods.

[0029] A variety of approaches have been taken to produce and screen combinatorial chemical libraries. Examples include methods in which the individual members of the library are physically separated from each other, such as when a single compound is synthesized in each of a multitude of reaction vessels. However, these libraries are typically screened one compound at a time, or at most, several compounds at a time and do not, therefore, result in the most efficient screening process. In other methods, compounds are synthesized on solid supports. Such solid supports include chips in which specific compounds occupy specific regions of the chip or membrane ("position addressable"). In other methods, compounds are synthesized on beads, with each bead containing a different chemical structure.

[0030] Two difficulties that arise in screening large libraries are (1) the number of distinct compounds that can be screened; and (2) the identification of compounds which are active in the screen. In one method, the compounds which are active in the screen are identified by narrowing the original library into ever smaller fractions and subfractions, in each case selecting the fraction or subfraction which contains active compounds and further subdividing until attaining an active subfraction which contains a set of compounds which is sufficiently small that all members of the subset can be individually synthesized and assessed for the desired activity. This is a tedious and time consuming activity.

[0031] Another method of deconvoluting the results of a combinatorial library screen is to utilize libraries in which the library members are tagged with an identifying label, that is, each label present in the library is associated with a discreet compound structure present in the library, such that identification of the label tells the structure of the tagged molecule. One approach to tagged libraries utilizes oligonucleotide tags, as described, for example, in U.S. Pat. Nos. 5,573,905; 5,708,153; 5,723,598, 6,060,596 published PCT applications WO 93/06121; WO 93/20242; WO 94/13623; WO 00/23458; WO 02/074929 and WO 02/103008, and by Brenner and Lerner (Proc. Natl. Acad. Sci. USA 89, 5381-5383 (1992); Nielsen and Janda (Methods: A Companion to Methods in Enzymology 6, 361-371 (1994); and Nielsen, Brenner and Janda (J. Am. Chem. Soc. 115, 9812-9813 (1993)), each of which is incorporated herein by reference in its entirety. Such tags can be amplified, using for example, polymerase chain reaction, to produce many copies of the tag and identify the tag by sequencing. The sequence of the tag then identifies the structure of the binding molecule, which can be synthesized in pure form and tested. To date, there has been no report of the use of the methodology disclosed by Lerner et al. to prepare large libraries. The present invention provides an improvement in methods to produce DNA-encoded libraries, as well as the first examples of large (10.sup.5 members or greater) libraries of DNA-encoded molecules in which the functional moiety is synthesized using solution phase synthetic methods.

[0032] The present invention provides methods which enable facile synthesis of oligonucleotide-encoded combinatorial libraries, and permit an efficient, high-fidelity means of adding such an oligonucleotide tag to each member of a vast collection of molecules.

[0033] The methods of the invention include methods for synthesizing bifunctional molecules which comprise a first moiety ("functional moiety") which is made up of building blocks, and a second moiety operatively linked to the first moiety, comprising an oligonucleotide tag which identifies the structure of the first moiety, i.e., the oligonucleotide tag indicates which building blocks were used in the construction of the first moiety, as well as the order in which the building blocks were linked. Generally, the information provided by the oligonucleotide tag is sufficient to determine the building blocks used to construct the active moiety. In certain embodiments, the sequence of the oligonucleotide tag is sufficient to determine the arrangement of the building blocks in the functional moiety, for example, for peptidic moieties, the amino acid sequence.

[0034] The term "functional moiety" as used herein, refers to a chemical moiety comprising one or more building blocks. Preferably, the building blocks in the functional moiety are not nucleic acids. The functional moiety can be a linear or branched or cyclic polymer or oligomer or a small organic molecule.

[0035] The term "building block", as used herein, is a chemical structural unit which is linked to other chemical structural units or can be linked to other such units. When the functional moiety is polymeric or oligomeric, the building blocks are the monomeric units of the polymer or oligomer. Building blocks can also include a scaffold structure ("scaffold building block") to which is, or can be, attached one or more additional structures ("peripheral building blocks").

[0036] It is to be understood that the term "building block" is used herein to refer to a chemical structural unit as it exists in a functional moiety and also in the reactive form used for the synthesis of the functional moiety. Within the functional moiety, a building block will exist without any portion of the building block which is lost as a consequence of incorporating the building block into the functional moiety. For example, in cases in which the bond-forming reaction releases a small molecule (see below), the building block as it exists in the functional moiety is a "building block residue", that is, the remainder of the building block used in the synthesis following loss of the atoms that it contributes to the released molecule.

[0037] The building blocks can be any chemical compounds which are complementary, that is the building blocks must be able to react together to form a structure comprising two or more building blocks. Typically, all of the building blocks used will have at least two reactive groups, although it is possible that some of the building blocks (for example the last building block in an oligomeric functional moiety) used will have only one reactive group each. Reactive groups on two different building blocks should be complementary, i.e., capable of reacting together to form a covalent bond, optionally with the concomitant loss of a small molecule, such as water, HCl, HF, and so forth.

[0038] For the present purposes, two reactive groups are complementary if they are capable of reacting together to form a covalent bond. In a preferred embodiment, the bond forming reactions occur rapidly under ambient conditions without substantial formation of side products. Preferably, a given reactive group will react with a given complementary reactive group exactly once. In one embodiment, complementary reactive groups of two building blocks react, for example, via nucleophilic substitution, to form a covalent bond. In one embodiment, one member of a pair of complementary reactive groups is an electrophilic group and the other member of the pair is a nucleophilic group.

[0039] Complementary electrophilic and nucleophilic groups include any two groups which react via nucleophilic substitution under suitable conditions to form a covalent bond. A variety of suitable bond-forming reactions are known in the art. See, for example, March, Advanced Organic Chemistry, fourth edition, New York: John Wiley and Sons (1992), Chapters 10 to 16; Carey and Sundberg, Advanced Organic Chemistry, Part B, Plenum (1990), Chapters 1-11; and Coltman et al., Principles and Applications of Organotransition Metal Chemistry, University Science Books, Mill Valley, Calif. (1987), Chapters 13 to 20; each of which is incorporated herein by reference in its entirety. Examples of suitable electrophilic groups include reactive carbonyl groups, such as acyl chloride groups, ester groups, including carbonyl pentafluorophenyl esters and succinimide esters, ketone groups and aldehyde groups; reactive sulfonyl groups, such as sulfonyl chloride groups, and reactive phosphonyl groups. Other electrophilic groups include terminal epoxide groups, isocyanate groups and alkyl halide groups. Suitable nucleophilic groups include primary and secondary amino groups and hydroxyl groups and carboxyl groups.

[0040] Suitable complementary reactive groups are set forth below. One of skill in the art can readily determine other reactive group pairs that can be used in the present method, and the examples provided herein are not intended to be limiting.

[0041] In a first embodiment, the complementary reactive groups include activated carboxyl groups, reactive sulfonyl groups or reactive phosphonyl groups, or a combination thereof, and primary or secondary amino groups. In this embodiment, the complementary reactive groups react under suitable conditions to form an amide, sulfonamide or phosphonamidate bond.

[0042] In a second embodiment, the complementary reactive groups include epoxide groups and primary or secondary amino groups. An epoxide-containing building block reacts with an amine-containing building block under suitable conditions to form a carbon-nitrogen bond, resulting in a .beta.-amino alcohol.

[0043] In another embodiment, the complementary reactive groups include aziridine groups and primary or secondary amino groups. Under suitable conditions, an aziridine-containing building block reacts with an amine-containing building block to form a carbon-nitrogen bond, resulting in a 1,2-diamine. In a third embodiment, the complementary reactive groups include isocyanate groups and primary or secondary amino groups. An isocyanate-containing building block will react with an amino-containing building block under suitable conditions to form a carbon-nitrogen bond, resulting in a urea group.

[0044] In a fourth embodiment, the complementary reactive groups include isocyanate groups and hydroxyl groups. An isocyanate-containing building block will react with an hydroxyl-containing building block under suitable conditions to form a carbon-oxygen bond, resulting in a carbamate group.

[0045] In a fifth embodiment, the complementary reactive groups include amino groups and carbonyl-containing groups, such as aldehyde or ketone groups. Amines react with such groups via reductive amination to form a new carbon-nitrogen bond.

[0046] In a sixth embodiment, the complementary reactive groups include phosphorous ylide groups and aldehyde or ketone groups. A phosphorus-ylide-containing building block will react with an aldehyde or ketone-containing building block under suitable conditions to form a carbon-carbon double bond, resulting in an alkene.

[0047] In a seventh embodiment, the complementary reactive groups react via cycloaddition to form a cyclic structure. One example of such complementary reactive groups are alkynes and organic azides, which react under suitable conditions to form a triazole ring structure. An example of the use of this reaction to link two building blocks is illustrated in FIG. 8. Suitable conditions for such reactions are known in the art and include those disclosed in WO 03/101972, the entire contents of which are incorporated by reference herein.

[0048] In an eighth embodiment, the complementary reactive groups are an alkyl halide and a nucleophile, such as an amino group, a hydroxyl group or a carboxyl group. Such groups react under suitable conditions to form a carbon-nitrogen (alkyl halide plus amine) or carbon oxygen (alkyl halide plus hydroxyl or carboxyl group).

[0049] In a ninth embodiment, the complementary functional groups are a halogenated heteroaromatic group and a nucleophile, and the building blocks are linked under suitable conditions via aromatic nucleophilic substitution. Suitable halogenated heteroaromatic groups include chlorinated pyrimidines, triazines and purines, which react with nucleophiles, such as amines, under mild conditions in aqueous solution. Representative examples of the reaction of an oligonucleotide-tagged trichlorotriazine with amines are shown in FIGS. 9 and 10. Examples of suitable chlorinated heteroaromatic groups are shown in FIG. 11.

[0050] It is to be understood that the synthesis of a functional moiety can proceed via one particular type of coupling reaction, such as, but not limited to, one of the reactions discussed above, or via a combination of two or more coupling reactions, such as two or more of the coupling reactions discussed above. For example, in one embodiment, the building blocks are joined by a combination of amide bond formation (amino and carboxylic acid complementary groups) and reductive amination (amino and aldehyde or ketone complementary groups). Any coupling chemistry can be used, provided that it is compatible with the presence of an oligonucleotide. Double stranded (duplex) oligonucleotide tags, as used in certain embodiments of the present invention, are chemically more robust than single stranded tags, and, therefore, tolerate a broader range of reaction conditions and enable the use of bond-forming reactions that would not be possible with single-stranded tags.

[0051] A building block can include one or more functional groups in addition to the reactive group or groups employed to form the functional moiety. One or more of these additional functional groups can be protected to prevent undesired reactions of these functional groups. Suitable protecting groups are known in the art for a variety of functional groups (Greene and Wuts, Protective Groups in Organic Synthesis, second edition, New York: John Wiley and Sons (1991), incorporated herein by reference). Particularly useful protecting groups include t-butyl esters and ethers, acetals, trityl ethers and amines, acetyl esters, trimethylsilyl ethers, trichloroethyl ethers and esters and carbamates.

[0052] In one embodiment, each building block comprises two reactive groups, which can be the same or different. For example, each building block added in cycle s can comprise two reactive groups which are the same, but which are both complementary to the reactive groups of the building blocks added at steps s-1 and s+1. In another embodiment, each building block comprises two reactive groups which are themselves complementary. For example, a library comprising polyamide molecules can be produced via reactions between building blocks comprising two primary amino groups and building blocks comprising two activated carboxyl groups. In the resulting compounds there is no N- or C-terminus, as alternate amide groups have opposite directionality. Alternatively, a polyamide library can be produced using building blocks that each comprise an amino group and an activated carboxyl group. In this embodiment, the building blocks added in step n of the cycle will have a free reactive group which is complementary to the available reactive group on the n-1 building block, while, preferably, the other reactive group on the nth building block is protected. For example, if the members of the library are synthesized from the C to N direction, the building blocks added will comprise an activated carboxyl group and a protected amino group.

[0053] The functional moieties can be polymeric or oligomeric moieties, such as peptides, peptidomimetics, peptide nucleic acids or peptoids, or they can be small non-polymeric molecules, for example, molecules having a structure comprising a central scaffold and structures arranged about the periphery of the scaffold. Linear polymeric or oligomeric libraries will result from the use of building blocks having two reactive groups, while branched polymeric or oligomeric libraries will result from the use of building blocks having three or more reactive groups, optionally in combination with building blocks having only two reactive groups. Such molecules can be represented by the general formula X.sub.1X.sub.2 . . . X.sub.n, where each X is a monomeric unit of a polymer comprising n monomeric units, where n is an integer greater than 1 In the case of oligomeric or polymeric compounds, the terminal building blocks need not comprise two functional groups. For example, in the case of a polyamide library, the C-terminal building block can comprise an amino group, but the presence of a carboxyl group is optional. Similarly, the building block at the N-terminus can comprise a carboxyl group, but need not contain an amino group.

[0054] Branched oligomeric or polymeric compounds can also be synthesized provided that at least one building block comprises three functional groups which are reactive with other building blocks. A library of the invention can comprise linear molecules, branched molecules or a combination thereof.

[0055] Libraries can also be constructed using, for example, a scaffold building block having two or more reactive groups, in combination with other building blocks having only one available reactive group, for example, where any additional reactive groups are either protected or not reactive with the other reactive groups present in the scaffold building block. In one embodiment, for example, the molecules synthesized can be represented by the general formula X(Y).sub.n, where X is a scaffold building block; each Y is a building block linked to X and n is an integer of at least two, and preferably an integer from 2 to about 6. In one preferred embodiment, the initial building block of cycle 1 is a scaffold building block. In molecules of the formula X(Y).sub.n, each Y can be the same or different, but in most members of a typical library, each Y will be different.

[0056] In one embodiment, the libraries of the invention comprise polyamide compounds. The polyamide compounds can be composed of building blocks derived from any amino acids, including the twenty naturally occurring .alpha.-amino acids, such as alanine (Ala; A), glycine (Gly; G), asparagine (Asn; N), aspartic acid (Asp; D), glutamic acid (Glu; E), histidine (His; H), leucine (Leu; L), lysine (Lys; K), phenylalanine (Phe; F), tyrosine (Tyr; Y), threonine (Thr; T), serine (Ser; S), arginine (Arg; R), valine (Val; V), glutamine (Gln; Q), isoleucine (Ile; I), cysteine (Cys; C), methionine (Met; M), proline (Pro; P) and tryptophan (Trp; W), where the three-letter and one-letter codes for each amino acid are given. In their naturally occurring form, each of the foregoing amino acids exists in the L-configuration, which is to be assumed herein unless otherwise noted. In the present method, however, the D-configuration forms of these amino acids can also be used. These D-amino acids are indicated herein by lower case three- or one-letter code, i.e., ala (a), gly (g), leu (l), gln (q), thr (t), ser (s), and so forth. The building blocks can also be derived from other .alpha.-amino acids, including, but not limited to, 3-arylalanines, such as naphthylalanine, phenyl-substituted phenylalanines, including 4-fluoro-, 4-chloro, 4-bromo and 4-methylphenylalanine; 3-heteroarylalanines, such as 3-pyridylalanine, 3-thienylalanine, 3-quinolylalanine, and 3-imidazolylalanine; ornithine; citrulline; homocitrulline; sarcosine; homoproline; homocysteine; substituted proline, such as hydroxyproline and fluoroproline; dehydroproline; norleucine; O-methyltyrosine; O-methylserine; O-methylthreonine and 3-cyclohexylalanine. Each of the preceding amino acids can be utilized in either the D- or L-configuration.

[0057] The building blocks can also be amino acids which are not .alpha.-amino acids, such as .alpha.-azamino acids; .beta., .gamma., .delta., .epsilon.,-amino acids, and N-substituted amino acids, such as N-substituted glycine, where the N-substituent can be, for example, a substituted or unsubstituted alkyl, aryl, heteroaryl, arylalkyl or heteroarylalkyl group. In one embodiment, the N-substituent is a side chain from a naturally-occurring or non-naturally occurring .alpha.-amino acid.

[0058] The building block can also be a peptidomimetic structure, such as a dipeptide, tripeptide, tetrapeptide or pentapeptide mimetic. Such peptidomimetic building blocks are preferably derived from amino acyl compounds, such that the chemistry of addition of these building blocks to the growing poly(aminoacyl) group is the same as, or similar to, the chemistry used for the other building blocks. The building blocks can also be molecules which are capable of forming bonds which are isosteric with a peptide bond, to form peptidomimetic functional moieties comprising a peptide backbone modification, such as .psi.[CH.sub.2S], .psi.[CH.sub.2NH], .psi.[CSNH.sub.2], .psi.[NHCO], .psi.[COCH.sub.2], and .psi.[(E) or (Z) CH.dbd.CH]. In the nomenclature used above, .psi. indicates the absence of an amide bond. The structure that replaces the amide group is specified within the brackets.

[0059] In one embodiment, the invention provides a method of synthesizing a compound comprising or consisting of a functional moiety which is operatively linked to an encoding oligonucleotide. The method includes the steps of: (1) providing an initiator compound consisting of an initial functional moiety comprising n building blocks, where n is an integer of 1 or greater, wherein the initial functional moiety comprises at least one reactive group, and wherein the initial functional moiety is operatively linked to an initial oligonucleotide which encodes the n building blocks; (2) reacting the initiator compound with a building block comprising at least one complementary reactive group, wherein the at least one complementary reactive group is complementary to the reactive group of step (1), under suitable conditions for reaction of the reactive group and the complementary reactive group to form a covalent bond; (3) reacting the initial oligonucleotide with an incoming oligonucleotide in the presence of an enzyme which catalyzes ligation of the initial oligonucleotide and the incoming oligonucleotide, under conditions suitable for ligation of the incoming oligonucleotide and the initial oligonucleotide, thereby producing a molecule which comprises or consists of a functional moiety comprising n+1 building blocks which is operatively linked to an encoding oligonucleotide. If the functional moiety of step (3) comprises a reactive group, steps 1-3 can be repeated one or more times, thereby forming cycles 1 to i, where i is an integer of 2 or greater, with the product of step (3) of a cycle s-1, where s is an integer of i or less, becoming the initiator compound of step (1) of cycle s. In each cycle, one building block is added to the growing functional moiety and one oligonucleotide sequence, which encodes the new building block, is added to the growing encoding oligonucleotide.

[0060] In a preferred embodiment, each individual building block is associated with a distinct oligonucleotide, such that the sequence of nucleotides in the oligonucleotide added in a given cycle identifies the building block added in the same cycle.

[0061] The coupling of building blocks and ligation of oligonucleotides will generally occur at similar concentrations of starting materials and reagents. For example, concentrations of reactants on the order of micromolar to millimolar, for example from about 10 .mu.M to about 10 mM, are preferred in order to have efficient coupling of building blocks.

[0062] In certain embodiments, the method further comprises, following step (2), the step of scavenging any unreacted initial functional moiety. Scavenging any unreacted initial functional moiety in a particular cycle prevents the initial functional moiety of the cycle from reacting with a building block added in a later cycle. Such reactions could lead to the generation of functional moieties missing one or more building blocks, potentially leading to a range of functional moiety structures which correspond to a particular oligonucleotide sequence. Such scavenging can be accomplished by reacting any remaining initial functional moiety with a compound which reacts with the reactive group of step (2). Preferably, the scavenger compound reacts rapidly with the reactive group of step (2) and includes no additional reactive groups that can react with building blocks added in later cycles. For example, in the synthesis of a compound where the reactive group of step (2) is an amino group, a suitable scavenger compound is an N-hydroxysuccinimide ester, such as acetic acid N-hydroxysuccinimide ester.

[0063] In another embodiment, the invention provides a method of producing a library of compounds, wherein each compound comprises a functional moiety comprising two or more building block residues which is operatively linked to an oligonucleotide. In a preferred embodiment, the oligonucleotide present in each molecule provides sufficient information to identify the building blocks within the molecule and, optionally, the order of addition of the building blocks. In this embodiment, the method of the invention comprises a method of synthesizing a library of compounds, wherein the compounds comprise a functional moiety comprising two or more building blocks which is operatively linked to an oligonucleotide which identifies the structure of the functional moiety. The method comprises the steps of (1) providing a solution comprising m initiator compounds, wherein m is an integer of 1 or greater, where the initiator compounds consist of a functional moiety comprising n building blocks, where n is an integer of 1 or greater, which is operatively linked to an initial oligonucleotide which identifies the n building blocks; (2) dividing the solution of step (1) into at least r fractions, wherein r is an integer of 2 or greater; (3) reacting each fraction with one of r building blocks, thereby producing r fractions comprising compounds consisting of a functional moiety comprising n+1 building blocks operatively linked to the initial oligonucleotide; (4) reacting each of the r fractions of step (3) with one of a set of r distinct incoming oligonucleotides under conditions suitable for enzymatic ligation of the incoming oligonucleotide to the initial oligonucleotide, thereby producing r fractions comprising molecules consisting of a functional moiety comprising n+1 building blocks operatively linked to an elongated oligonucleotide which encodes the n+1 building blocks. Optionally, the method can further include the step of (5) recombining the r fractions, produced in step (4), thereby producing a solution comprising molecules consisting of a functional moiety comprising n+1 building blocks, which is operatively linked to an elongated oligonucleotide which encodes the n+1 building blocks. Steps (1) to (5) can be conducted one or more times to yield cycles 1 to i, where i is an integer of 2 or greater. In cycle s+1, where s is an integer of i-1 or less, the solution comprising m initiator compounds of step (1) is the solution of step (5) of cycle s. Likewise, the initiator compounds of step (1) of cycle s+1 are the products of step (4) in cycle s.

[0064] Preferably the solution of step (2) is divided into r fractions in each cycle of the library synthesis. In this embodiment, each fract is reated with a unique building block.

[0065] In the methods of the invention, the order of addition of the building block and the incoming oligonucleotide is not critical, and steps (2) and (3) of the synthesis of a molecule, and steps (3) and (4) in the library synthesis can be reversed, i.e., the incoming oligonucleotide can be ligated to the initial oligonucleotide before the new building block is added. In certain embodiments, it may be possible to conduct these two steps simultaneously.

[0066] In certain embodiments, the method further comprises, following step (2), the step of scavenging any unreacted initial functional moiety. Scavenging any unreacted initial functional moiety in a particular cycle prevents the initial functional moiety of a the cycle from reacting with a building block added in a later cycle. Such reactions could lead to the generation of functional moieties missing one or more building blocks, potentially leading to a range of functional moiety structures which correspond to a particular oligonucleotide sequence. Such scavenging can be accomplished by reacting any remaining initial functional moiety with a compound which reacts with the reactive group of step (2). Preferably, the scavenger compound reacts rapidly with the reactive group of step (2) and includes no additional reactive groups that can react with building blocks added in later cycles. For example, in the synthesis of a compound where the reactive group of step (2) is an amino group, a suitable scavenger compound is an N-hydroxysuccinimide ester, such as acetic acid N-hydroxysuccinimide ester.

[0067] In one embodiment, the building blocks used in the library synthesis are selected from a set of candidate building blocks by evaluating the ability of the candidate building blocks to react with appropriate complementary functional groups under the conditions used for synthesis of the library. Building blocks which are shown to be suitably reactive under such conditions can then be selected for incorporation into the library. The products of a given cycle can, optionally, be purified. When the cycle is an intermediate cycle, i.e., any cycle prior to the final cycle, these products are intermediates and can be purified prior to initiation of the next cycle. If the cycle is the final cycle, the products of the cycle are the final products, and can be purified prior to any use of the compounds. This purification step can, for example, remove unreacted or excess reactants and the enzyme employed for oligonucleotide ligation. Any methods which are suitable for separating the products from other species present in solution can be used, including liquid chromatography, such as high performance liquid chromatography (HPLC) and precipitation with a suitable co-solvent, such as ethanol. Suitable methods for purification will depend upon the nature of the products and the solvent system used for synthesis.

[0068] The reactions are, preferably, conducted in aqueous solution, such as a buffered aqueous solution, but can also be conducted in mixed aqueous/organic media consistent with the solubility properties of the building blocks, the oligonucleotides, the intermediates and final products and the enzyme used to catalyze the oligonucleotide ligation.

[0069] It is to be understood that the theoretical number of compounds produced by a given cycle in the method described above is the product of the number of different initiator compounds, m, used in the cycle and the number of distinct building blocks added in the cycle, r. The actual number of distinct compounds produced in the cycle can be as high as the product of r and m (r.times.m), but could be lower, given differences in reactivity of certain building blocks with certain other building blocks. For example, the kinetics of addition of a particular building block to a particular initiator compound may be such that on the time scale of the synthetic cycle, little to none of the product of that reaction may be produced.

[0070] In certain embodiments, a common building block is added prior to cycle 1, following the last cycle or in between any two cycles. For example, when the functional moiety is a polyamide, a common N-terminal capping building block can be added after the final cycle. A common building block can also be introduced between any two cycles, for example, to add a functional group, such as an alkyne or azide group, which can be utilized to modify the functional moieties, for example by cyclization, following library synthesis.

[0071] The term "operatively linked", as used herein, means that two chemical structures are linked together in such a way as to remain linked through the various manipulations they are expected to undergo. Typically the functional moiety and the encoding oligonucleotide are linked covalently via an appropriate linking group. The linking group is a bivalent moiety with a site of attachment for the oligonucleotide and a site of attachment for the functional moiety. For example, when the functional moiety is a polyamide compound, the polyamide compound can be attached to the linking group at its N-terminus, its C-terminus or via a functional group on one of the side chains. The linking group is sufficient to separate the polyamide compound and the oligonucleotide by at least one atom, and preferably, by more than one atom, such as at least two, at least three, at least four, at least five or at least six atoms. Preferably, the linking group is sufficiently flexible to allow the polyamide compound to bind target molecules in a manner which is independent of the oligonucleotide.

[0072] In one embodiment, the linking group is attached to the N-terminus of the polyamide compound and the 5'-phosphate group of the oligonucleotide. For example, the linking group can be derived from a linking group precursor comprising an activated carboxyl group on one end and an activated ester on the other end. Reaction of the linking group precursor with the N-terminal nitrogen atom will form an amide bond connecting the linking group to the polyamide compound or N-terminal building block, while reaction of the linking group precursor with the 5'-hydroxy group of the oligonucleotide will result in attachment of the oligonucleotide to the linking group via an ester linkage. The linking group can comprise, for example, a polymethylene chain, such as a --(CH.sub.2).sub.n-chain or a poly(ethylene glycol) chain, such as a --(CH.sub.2CH.sub.2O).sub.n chain, where in both cases n is an integer from 1 to about 20. Preferably, n is from 2 to about 12, more preferably from about 4 to about 10. In one embodiment, the linking group comprises a hexamethylene (--(CH.sub.2).sub.6--) group.

[0073] When the building blocks are amino acid residues, the resulting functional moiety is a polyamide. The amino acids can be coupled using any suitable chemistry for the formation of amide bonds. Preferably, the coupling of the amino acid building blocks is conducted under conditions which are compatible with enzymatic ligation of oligonucleotides, for example, at neutral or near-neutral pH and in aqueous solution. In one embodiment, the polyamide compound is synthesized from the C-terminal to N-terminal direction. In this embodiment, the first, or C-terminal, building block is coupled at its carboxyl group to an oligonucleotide via a suitable linking group. The first building block is reacted with the second building block, which preferably has an activated carboxyl group and a protected amino group. Any activating/protecting group strategy which is suitable for solution phase amide bond formation can be used. For example, suitable activated carboxyl species include acyl fluorides (U.S. Pat. No. 5,360,928, incorporated herein by reference in its entirety), symmetrical anhydrides and N-hydroxysuccinimide esters. The acyl groups can also be activated in situ, as is known in the art, by reaction with a suitable activating compound. Suitable activating compounds include dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIC), 1-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), n-propane-phosphonic anhydride (PPA), N,N-bis(2-oxo-3-oxazolidinyl)imido-phosphoryl chloride (BOP-Cl), bromo-tris-pyrrolidinophosphonium hexafluorophosphate (PyBrop), diphenylphosphoryl azide (DPPA), Castro's reagent (BOP, PyBop), O-benzotriazolyl-N,N,N',N'-tetramethyluronium salts (HBTU), diethylphosphoryl cyanide (DEPCN), 2,5-diphenyl-2,3-dihydro-3-oxo-4-hydroxy-thiophene dioxide (Steglich's reagent; HOTDO), 1,1'-carbonyl-diimidazole (CDI), and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM). The coupling reagents can be employed alone or in combination with additives such as N. N-dimethyl-4-aminopyridine (DMAP), N-hydroxy-benzotriazole (HOBt), N-hydroxybenzotriazine (HOOBt), N-hydroxysuccinimide (HOSu) N-hydroxyazabenzotriazole (HOAt), azabenzotriazolyl-tetramethyluronium salts (HATU, HAPyU) or 2-hydroxypyridine. In certain embodiments, synthesis of a library requires the use of two or more activation strategies, to enable the use of a structurally diverse set of building blocks. For each building block, one skilled in the art can determine the appropriate activation strategy.

[0074] The N-terminal protecting group can be any protecting group which is compatible with the conditions of the process, for example, protecting groups which are suitable for solution phase synthesis conditions. A preferred protecting group is the fluorenylmethoxycarbonyl ("Fmoc") group. Any potentially reactive functional groups on the side chain of the aminoacyl building block may also need to be suitably protected. Preferably the side chain protecting group is orthogonal to the N-terminal protecting group, that is, the side chain protecting group is removed under conditions which are different than those required for removal of the N-terminal protecting group. Suitable side chain protecting groups include the nitroveratryl group, which can be used to protect both side chain carboxyl groups and side chain amino groups. Another suitable side chain amine protecting group is the N-pent-4-enoyl group.

[0075] The building blocks can be modified following incorporation into the functional moiety, for example, by a suitable reaction involving a functional group on one or more of the building blocks. Building block modification can take place following addition of the final building block or at any intermediate point in the synthesis of the functional moiety, for example, after any cycle of the synthetic process. When a library of bifunctional molecules of the invention is synthesized, building block modification can be carried out on the entire library or on a portion of the library, thereby increasing the degree of complexity of the library. Suitable building block modifying reactions include those reactions that can be performed under conditions compatible with the functional moiety and the encoding oligonucleotide. Examples of such reactions include acylation and sulfonation of amino groups or hydroxyl groups, alkylation of amino groups, esterification or thioesterification of carboxyl groups, amidation of carboxyl groups, epoxidation of alkenes, and other reactions as are known the art. When the functional moiety includes a building block having an alkyne or an azide functional group, the azide/alkyne cycloaddition reaction can be used to derivatize the building block. For example, a building block including an alkyne can be reacted with an organic azide, or a building block including an azide can be reacted with an alkyne, in either case forming a triazole. Building block modification reactions can take place after addition of the final building block or at an intermediate point in the synthetic process, and can be used to append a variety of chemical structures to the functional moiety, including carbohydrates, metal binding moieties and structures for targeting certain biomolecules or tissue types.

[0076] In another embodiment, the functional moiety comprises a linear series of building blocks and this linear series is cyclized using a suitable reaction. For example, if at least two building blocks in the linear array include sulfhydryl groups, the sulfhydryl groups can be oxidized to form a disulfide linkage, thereby cyclizing the linear array. For example, the functional moieties can be oligopeptides which include two or more L or D-cysteine and/or L or D-homocysteine moieties. The building blocks can also include other functional groups capable of reacting together to cyclize the linear array, such as carboxyl groups and amino or hydroxyl groups.

[0077] In a preferred embodiment, one of the building blocks in the linear array comprises an alkyne group and another building block in the linear array comprises an azide group. The azide and alkyne groups can be induced to react via cycloaddition, resulting in the formation of a macrocyclic structure. In the example illustrated in FIG. 9, the functional moiety is a polypeptide comprising a propargylglycine building block at its C-terminus and an azidoacetyl group at its N-terminus. Reaction of the alkyne and the azide group under suitable conditions results in formation of a cyclic compound, which includes a triazole structure within the macrocycle. In the case of a library, in one embodiment, each member of the library comprises alkyne- and azide-containing building blocks and can be cyclized in this way. In a second embodiment, all members of the library comprises alkyne- and azide-containing building blocks, but only a portion of the library is cyclized. In a third embodiment, only certain functional moieties include alkyne- and azide-containing building blocks, and only these molecules are cyclized. In the forgoing second and third embodiments, the library, following the cycloaddition reaction, will include both cyclic and linear functional moieties.

[0078] The oligonucleotides are ligated using enzymatic methods. In one embodiment, the initial building block is operatively linked to an initial oligonucleotide. Prior to or following coupling of a second building block to the initial building block, a second oligonucleotide sequence which identifies the second building block is ligated to the initial oligonucleotide. Methods for ligating the initial oligonucleotide sequence and the incoming oligonucleotide sequence are set forth in FIGS. 1 and 2. In FIG. 1, the initial oligonucleotide is double-stranded, and one strand includes an overhang sequence which is complementary to one end of the second oligonucleotide and brings the second oligonucleotide into contact with the initial oligonucleotide. Preferably the overhanging sequence of the initial oligonucleotide and the complementary sequence of the second oligonucleotide are both at least about 4 bases; more preferably both sequences are both the same length. The initial oligonucleotide and the second oligonucleotide can be ligated using a suitable enzyme. If the initial oligonucleotide is linked to the first building block at the 5' end of one of the strands (the "top strand"), then the strand which is complementary to the top strand (the "bottom strand") will include the overhang sequence at its 5' end, and the second oligonucleotide will include a complementary sequence at its 5' end. Following ligation of the second oligonucleotide, a strand can be added which is complementary to the sequence of the second oligonucleotide which is 3' to the overhang complementary sequence, and which includes additional overhang sequence.

[0079] In one embodiment, the oligonucleotide is elongated as set forth in FIG. 2. The oligonucleotide bound to the growing functional moiety and the incoming oligonucleotide are positioned for ligation by the use of a "splint" sequence, which includes a region which is complementary to the 3' end of the initial oligonucleotide and a region which is complementary to the 5' end of the incoming oligonucleotide. The splint brings the 5' end of the oligonucleotide into proximity with the 3' end of the incoming oligo and ligation is accomplished using enzymatic ligation. In the example illustrated in FIG. 2, the initial oligonucleotide consists of 16 nucleobases and the splint is complementary to the 6 bases at the 3' end. The incoming oligonucleotide consists of 12 nucleobases, and the splint is complementary to the 6 bases at the 5' terminus. The length of the splint and the lengths of the complementary regions are not critical. However, the complementary regions should be sufficiently long to enable stable dimer formation under the conditions of the ligation, but not so long as to yield an excessively large encoding nucleotide in the final molecules. It is preferred that the complementary regions are from about 4 bases to about 12 bases, more preferably from about 5 bases to about 10 bases, and most preferably from about 5 bases to about 8 bases in length.

[0080] In one embodiment, the initial oligonucleotide is double-stranded and the two strands are covalently joined. One means of covalently joining the two strands is shown in FIG. 3, in which a linking moiety is used to link the two strands and the functional moiety. The linking moiety can be any chemical structure which comprises a first functional group which is adapted to react with a building block, a second functional group which is adapted to react with the 3'-end of an oligonucleotide, and a third functional group which is adapted to react with the 5'-end of an oligonucleotide. Preferably, the second and third functional groups are oriented so as to position the two oligonucleotide strands in a relative orientation that permits hybridization of the two strands. For example, the linking moiety can have the general structure (I):

##STR00003##

where A, is a functional group that can form a covalent bond with a building block, B is a functional group that can form a bond with the 5'-end of an oligonucleotide, and C is a functional group that can form a bond with the 3'-end of an oligonucleotide. D, F and E are chemical groups that link functional groups A, C and B to S, which is a core atom or scaffold. Preferably, D, E and F are each independently a chain of atoms, such as an alkylene chain or an oligo(ethylene glycol) chain, and D, E and F can be the same or different, and are preferably effective to allow hybridization of the two oligonucleotides and synthesis of the functional moiety. In one embodiment, the trivalent linker has the structure

##STR00004##

In this embodiment, the NH group is available for attachment to a building block, while the terminal phosphate groups are available for attachment to an oligonucleotide.

[0081] In embodiments in which the initial oligonucleotide is double-stranded, the incoming oligonucleotides are also double-stranded. As shown in FIG. 3, the initial oligonucleotide can have one strand which is longer than the other, providing an overhang sequence. In this embodiment, the incoming oligonucleotide includes an overhang sequence which is complementary to the overhang sequence of the initial oligonucleotide. Hybridization of the two complementary overhang sequences brings the incoming oligonucleotide into position for ligation to the initial oligonucleotide. This ligation can be performed enzymatically using a DNA or RNA ligase. The overhang sequences of the incoming oligonucleotide and the initial oligonucleotide are preferably the same length and consist of two or more nucleotides, preferably from 2 to about 10 nucleotides, more preferably from 2 to about 6 nucleotides. In one preferred embodiment, the incoming oligonucleotide is a double-stranded oligonucleotide having an overhang sequence at each end. The overhang sequence at one end is complementary to the overhang sequence of the initial oligonucleotide, while, after ligation of the incoming oligonucleotide and the initial oligonucleotide, the overhang sequence at the other end becomes the overhang sequence of initial oligonucleotide of the next cycle. In one embodiment, the three overhang sequences are all 2 to 6 nucleotides in length, and the encoding sequence of the incoming oligonucleotide is from 3 to 10 nucleotides in length, preferably 3 to 6 nucleotides in length. In a particular embodiment, the overhang sequences are all 2 nucleotides in length and the encoding sequence is 5 nucleotides in length.

[0082] In the embodiment illustrated in FIG. 4, the incoming strand has a region at its 3' end which is complementary to the 3' end of the initial oligonucleotide, leaving overhangs at the 5' ends of both strands. The 5' ends can be filled in using, for example, a DNA polymerase, such as vent polymerase, resulting in a double-stranded elongated oligonucleotide. The bottom strand of this oligonucleotide can be removed, and additional sequence added to the 3' end of the top strand using the same method.

[0083] The encoding oligonucleotide tag is formed as the result of the successive addition of oligonucleotides that identify each successive building block. In one embodiment of the methods of the invention, the successive oligonucleotide tags may be coupled by enzymatic ligation to produce an encoding oligonucleotide.

[0084] Enzyme-catalyzed ligation of oligonucleotides can be performed using any enzyme that has the ability to ligate nucleic acid fragments. Exemplary enzymes include ligases, polymerases, and topoisomerases. In specific embodiments of the invention, DNA ligase (EC 6.5.1.1), DNA polymerase (EC 2.7.7.7), RNA polymerase (EC 2.7.7.6) or topoisomerase (EC 5.99.1.2) are used to ligate the oligonucleotides. Enzymes contained in each EC class can be found, for example, as described in Bairoch (2000) Nucleic Acids Research 28:304-5.

[0085] In a preferred embodiment, the oligonucleotides used in the methods of the invention are oligodeoxynucleotides and the enzyme used to catalyze the oligonucleotide ligation is DNA ligase. In order for ligation to occur in the presence of the ligase, i.e., for a phosphodiester bond to be formed between two oligonucleotides, one oligonucleotide must have a free 5' phosphate group and the other oligonucleotide must have a free 3' hydroxyl group. Exemplary DNA ligases that may be used in the methods of the invention include T4 DNA ligase, Taq DNA ligase, T.sub.4 RNA ligase, DNA ligase (E. coli) (all available from, for example, New England Biolabs, MA).

[0086] One of skill in the art will understand that each enzyme used for ligation has optimal activity under specific conditions, e.g., temperature, buffer concentration, pH and time. Each of these conditions can be adjusted, for example, according to the manufacturer's instructions, to obtain optimal ligation of the oligonucleotide tags.

[0087] The incoming oligonucleotide can be of any desirable length, but is preferably at least three nucleobases in length. More preferably, the incoming oligonucleotide is 4 or more nucleobases in length. In one embodiment, the incoming oligonucleotide is from 3 to about 12 nucleobases in length. It is preferred that the oligonucleotides of the molecules in the libraries of the invention have a common terminal sequence which can serve as a primer for PCR, as is known in the art. Such a common terminal sequence can be incorporated as the terminal end of the incoming oligonucleotide added in the final cycle of the library synthesis, or it can be added following library synthesis, for example, using the enzymatic ligation methods disclosed herein.

[0088] A preferred embodiment of the method of the invention is set forth in FIG. 5. The process begins with a synthesized DNA sequence which is attached at its 5' end to a linker which terminates in an amino group. In step 1, this starting DNA sequence is ligated to an incoming DNA sequence in the presence of a splint DNA strand, DNA ligase and dithiothreitol in Tris buffer. This yields a tagged DNA sequence which can then be used directly in the next step or purified, for example, using HPLC or ethanol precipitation, before proceeding to the next step. In step 2 the tagged DNA is reacted with a protected activated amino acid, in this example, an Fmoc-protected amino acid fluoride, yielding a protected amino acid-DNA conjugate. In step 3, the protected amino acid-DNA conjugate is deprotected, for example, in the presence of piperidine, and the resulting deprotected conjugate is, optionally, purified, for example, by HPLC or ethanol precipitation. The deprotected conjugate is the product of the first synthesis cycle, and becomes the starting material for the second cycle, which adds a second amino acid residue to the free amino group of the deprotected conjugate.

[0089] In embodiments in which PCR is to be used to amplify the encoding oligonucleotides of selected molecules, the encoding oligonucleotides preferably include PCR primer sequences. For example, a PCR primer sequence can be included in the initial oligonucleotide prior to the first cycle of synthesis, or it can be included with the first incoming oligonucleotide. The encoding oligonucleotide can also include a capping PCR primer sequence that follows the encoding sequences. The capping sequence can be ligated to the encoding oligonucleotide following the final cycle of library synthesis or it can be included in the incoming oligonucleotide of the final cycle. In cases in which the PCR primer sequences are included in an incoming oligonucleotide, these incoming oligonucleotides will preferably be significantly longer than the incoming oligonucleotides added in the other cycles, because they will include both an encoding sequence and a PCR primer sequence.

[0090] In cases in which the capping sequence is added after the addition of the final building block and final incoming oligonucleotide, the synthesis of a library as set forth herein will include the step of ligating the capping sequence to the encoding oligonucleotide, such that the oligonucleotide portion of substantially all of the library members terminates in a sequence that includes a PCR primer sequence. PCR primer sequences suitable for use in the libraries of the invention are known in the art; suitable primers and methods are set forth, for example, in Innis et al., eds., PCR Protocols: A Guide to Methods and Applications, San Diego: Academic Press (1990), the contents of which are incorporated herein by reference in their entirety. Preferably, the capping sequence is added by ligation to the pooled fractions which are products of the final synthetic cycle. The capping sequence can be added using the enzymatic process used in the construction of the library.

[0091] As indicated above, the nucleotide sequence of the oligonucleotide tag as part of the methods of this invention, may be determined by the use of the polymerase chain reaction (PCR).

[0092] The oligonucleotide tag is comprised of polynucleotides that identify the building blocks that make up the functional moiety as described herein. The nucleic acid sequence of the oligonucleotide tag is determined by subjecting the oligonucleotide tag to a PCR reaction as follows. The appropriate sample is contacted with a PCR primer pair, each member of the pair having a preselected nucleotide sequence. The PCR primer pair is capable of initiating primer extension reactions by hybridizing to a PCR primer binding site on the encoding oligonucleotide tag. The PCR primer binding site is preferably designed into the encoding oligonucleotide tag. For example, a PCR primer binding site may be incorporated into the initial oligonucleotide tag and the second PCR primer binding site may be in the final oligonucleotide tag. Alternatively, the second PCR primer binding site may be incorporated into the capping sequence as described herein. In preferred embodiments, the PCR primer binding site is at least about 5, 7, 10, 13, 15, 17, 20, 22, or 25 nucleotides in length.

[0093] The PCR reaction is performed by mixing the PCR primer pair, preferably a predetermined amount thereof, with the nucleic acids of the encoding oligonucleotide tag, preferably a predetermined amount thereof, in a PCR buffer to form a PCR reaction admixture. The admixture is thermocycled for a number of cycles, which is typically predetermined, sufficient for the formation of a PCR reaction product. A sufficient amount of product is one that can be isolated in a sufficient amount to allow for DNA sequence determination.

[0094] PCR is typically carried out by thermocycling i.e., repeatedly increasing and decreasing the temperature of a PCR reaction admixture within a temperature range whose lower limit is about 30.degree. C. to about 55.degree. C. and whose upper limit is about 90.degree. C. to about 100.degree. C. The increasing and decreasing can be continuous, but is preferably phasic with time periods of relative temperature stability at each of temperatures favoring polynucleotide synthesis, denaturation and hybridization.

[0095] The PCR reaction is performed using any suitable method. Generally it occurs in a buffered aqueous solution, i.e., a PCR buffer, preferably at a pH of 7-9. Preferably, a molar excess of the primer is present. A large molar excess is preferred to improve the efficiency of the process.

[0096] The PCR buffer also contains the deoxyribonucleotide triphosphates (polynucleotide synthesis substrates) dATP, dCTP, dGTP, and dTTP and a polymerase, typically thermostable, all in adequate amounts for primer extension (polynucleotide synthesis) reaction. The resulting solution (PCR admixture) is heated to about 90.degree. C.-100.degree. C. for about 1 to 10 minutes, preferably from 1 to 4 minutes. After this heating period the solution is allowed to cool to 54.degree. C., which is preferable for primer hybridization. The synthesis reaction may occur at a temperature ranging from room temperature up to a temperature above which the polymerase (inducing agent) no longer functions efficiently. Thus, for example, if DNA polymerase is used, the temperature is generally no greater than about 40.degree. C. The thermocycling is repeated until the desired amount of PCR product is produced. An exemplary PCR buffer comprises the following reagents: 50 mM KCl; 10 mM Tris-HCl at pH 8.3; 1.5 mM MgCl.sub.2; 0.001% (wt/vol) gelatin, 200 .mu.M dATP; 200 .mu.M dTTP; 200 .mu.M dCTP; 200 .mu.M dGTP; and 2.5 units Thermus aquaticus (Taq) DNA polymerase I per 100 microliters of buffer.

[0097] Suitable enzymes for elongating the primer sequences include, for example, E. coli DNA polymerase I, Taq DNA polymerase, Klenow fragment of E. coli DNA polymerase I, T4 DNA polymerase, other available DNA polymerases, reverse transcriptase, and other enzymes, including heat-stable enzymes, which will facilitate combination of the nucleotides in the proper manner to form the primer extension products which are complementary to each nucleic acid strand. Generally, the synthesis will be initiated at the 3' end of each primer and proceed in the 5' direction along the template strand, until synthesis terminates, producing molecules of different lengths.

[0098] The newly synthesized DNA strand and its complementary strand form a double-stranded molecule which can be used in the succeeding steps of the analysis process.

[0099] PCR amplification methods are described in detail in U.S. Pat. Nos. 4,683,192, 4,683,202, 4,800,159, and 4,965,188, and at least in PCR Technology: Principles and Applications for DNA Amplification, H. Erlich, ed., Stockton Press, New York (1989); and PCR Protocols: A Guide to Methods and Applications, Innis et al., eds., Academic Press, San Diego, Calif. (1990). The contents of all the foregoing documents are incorporated herein by reference.

[0100] The term "polynucleotide" as used herein in reference to primers, probes and nucleic acid fragments or segments to be synthesized by primer extension is defined as a molecule comprised of two or more deoxyribonucleotides, preferably more than three.

[0101] The term "primer" as used herein refers to a polynucleotide whether purified from a nucleic acid restriction digest or produced synthetically, which is capable of acting as a point of initiation of nucleic acid synthesis when placed under conditions in which synthesis of a primer extension product which is complementary to a nucleic acid strand is induced, i.e., in the presence of nucleotides and an agent for polymerization such as DNA polymerase, reverse transcriptase and the like, and at a suitable temperature and pH. The primer is preferably single stranded for maximum efficiency, but may alternatively be in double stranded form. If double stranded, the primer is first treated to separate it from its complementary strand before being used to prepare extension products. Preferably, the primer is a polydeoxyribonucleotide. The primer must be sufficiently long to prime the synthesis of extension products in the presence of the agents for polymerization. The exact lengths of the primers will depend on many factors, including temperature and the source of primer.

[0102] The primers used herein are selected to be "substantially" complementary to the different strands of each specific sequence to be amplified. This means that the primer must be sufficiently complementary so as to non-randomly hybridize with its respective template strand. Therefore, the primer sequence may or may not reflect the exact sequence of the template.

[0103] The polynucleotide primers can be prepared using any suitable method, such as, for example, the phosphotriester or phosphodiester methods described in Narang et al., (1979) Meth. Enzymol., 68:90; U.S. Pat. No. 4,356,270, U.S. Pat. No. 4,458,066, U.S. Pat. No. 4,416,988, U.S. Pat. No. 4,293,652; and Brown et al., (1979) Meth. Enzymol., 68:109. The contents of all the foregoing documents are incorporated herein by reference.

[0104] Once the encoding oligonucleotide tag has been amplified, the sequence of the tag, and ultimately the composition of the selected molecule, can be determined using nucleic acid sequence analysis, a well known procedure for determining the sequence of nucleotide sequences. Nucleic acid sequence analysis is approached by a combination of (a) physiochemical techniques, based on the hybridization or denaturation of a probe strand plus its complementary target, and (b) enzymatic reactions with polymerases.

[0105] The invention further relates to the compounds which can be produced using the methods of the invention, and collections of such compounds, either as isolated species or pooled to form a library of chemical structures. Compounds of the invention include compounds of the formula

##STR00005##

where X is a functional moiety comprising one or more building blocks, Z is an oligonucleotide attached at its 3' terminus to B and Y is an oligonucleotide which is attached to C at its 5' terminus. A is a functional group that forms a covalent bond with X, B is a functional group that forms a bond with the 3'-end of Z and C is a functional group that forms a bond with the 5'-end of Y. D, F and E are chemical groups that link functional groups A, C and B to S, which is a core atom or scaffold. Preferably, D, E and F are each independently a chain of atoms, such as an alkylene chain or an oligo(ethylene glycol) chain, and D, E and F can be the same or different, and are preferably effective to allow hybridization of the two oligonucleotides and synthesis of the functional moiety.

[0106] Preferably, Y and Z are substantially complementary and are oriented in the compound so as to enable Watson-Crick base pairing and duplex formation under suitable conditions. Y and Z are the same length or different lengths. Preferably, Y and Z are the same length, or one of Y and Z is from 1 to 10 bases longer than the other. In a preferred embodiment, Y and Z are each 10 or more bases in length and have complementary regions of ten or more base pairs. More preferably, Y and Z are substantially complementary throughout their length, i.e., they have no more than one mismatch per every ten base pairs. Most preferably, Y and Z are complementary throughout their length, i.e., except for any overhang region on Y or Z, the strands hybridize via Watson-Crick base pairing with no mismatches throughout their entire length.

[0107] S can be a single atom or a molecular scaffold. For example, S can be a carbon atom, a boron atom, a nitrogen atom or a phosphorus atom, or a polyatomic scaffold, such as a phosphate group or a cyclic group, such as a cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl or heteroaryl group. In one embodiment, the linker is a group of the structure

##STR00006##

where each of n, m and p is, independently, an integer from 1 to about 20, preferably from 2 to eight, and more preferably from 3 to 6. In one particular embodiment, the linker has the structure shown below.

##STR00007##

[0108] In one embodiment, the libraries of the invention include molecules consisting of a functional moiety composed of building blocks, where each functional moiety is operatively linked to an encoding oligonucleotide. The nucleotide sequence of the encoding oligonucleotide is indicative of the building blocks present in the functional moiety, and in some embodiments, the connectivity or arrangement of the building blocks. The invention provides the advantage that the methodology used to construct the functional moiety and that used to construct the oligonucleotide tag can be performed in the same reaction medium, preferably an aqueous medium, thus simplifying the method of preparing the library compared to methods in the prior art. In certain embodiments in which the oligonucleotide ligation steps and the building block addition steps can both be conducted in aqueous media, each reaction will have a different pH optimum. In these embodiments, the building block addition reaction can be conducted at a suitable pH and temperature in a suitable aqueous buffer. The buffer can then be exchanged for an aqueous buffer which provides a suitable pH for oligonucleotide ligation.

[0109] One advantage of the methods of the invention is that they can be used to prepare libraries comprising vast numbers of compounds. The ability to amplify encoding oligonucleotide sequences using known methods such as polymerase chain reaction ("PCR") means that selected molecules can be identified even if relatively few copies are recovered. This allows the practical use of very large libraries, which, as a consequence of their high degree of complexity, either comprise relatively few copies of any given library member, or require the use of very large volumes. For example, a library consisting of 10.sup.8 unique structures in which each structure has 1.times.10.sup.12 copies (about 1 picomole), requires about 100 L of solution at 1 .mu.M effective concentration. For the same library, if each member is represented by 1,000,000 copies, the volume required is 100 .mu.L at 1 .mu.M effective concentration.

[0110] In a preferred embodiment, the library comprises from about 10.sup.3 to about 10.sup.15 copies of each library member. Given differences in efficiency of synthesis among the library members, it is possible that different library members will have different numbers of copies in any given library. Therefore, although the number of copies of each member theoretically present in the library may be the same, the actual number of copies of any given library member is independent of the number of copies of any other member. More preferably, the compound libraries of the invention include at least about 10.sup.5, 10.sup.6 or 10.sup.7 copies of each library member, or of substantially all library members. By "substantially all" library members is meant at least about 85% of the members of the library, preferably at least about 90%, and more preferably at least about 95% of the members of the library.

[0111] Preferably, the library includes a sufficient number of copies of each member that multiple rounds (i.e., two or more) of selection against a biological target can be performed, with sufficient quantities of binding molecules remaining following the final round of selection to enable amplification of the oligonucleotide tags of the remaining molecules and, therefore, identification of the functional moieties of the binding molecules. A schematic representation of such a selection process is illustrated in FIG. 6, in which 1 and 2 represent library members, B is a target molecule and X is a moiety operatively linked to B that enables the removal of B from the selection medium. In this example, compound 1 binds to B, while compound 2 does not bind to B. The selection process, as depicted in Round 1, comprises (I) contacting a library comprising compounds 1 and 2 with B-X under conditions suitable for binding of compound 1 to B; (II) removing unbound compound 2, (III) dissociating compound 1 from B and removing BX from the reaction medium. The result of Round 1 is a collection of molecules that is enriched in compound 1 relative to compound 2. Subsequent rounds employing steps I-III result in further enrichment of compound 1 relative to compound 2. Although three rounds of selection are shown in FIG. 6, in practice any number of rounds may be employed, for example from one round to ten rounds, to achieve the desired enrichment of binding molecules relative to non-binding molecules.

[0112] In the embodiment shown in FIG. 6, there is no amplification (synthesis of more copies) of the compounds remaining after any of the rounds of selection. Such amplification can lead to a mixture of compounds which is not consistent with the relative amounts of the compounds remaining after the selection. This inconsistency is due to the fact that certain compounds may be more readily synthesized that other compounds, and thus may be amplified in a manner which is not proportional to their presence following selection. For example, if compound 2 is more readily synthesized than compound 1, the amplification of the molecules remaining after Round 2 would result in a disproportionate amplification of compound 2 relative to compound 1, and a resulting mixture of compounds with a much lower (if any) enrichment of compound 1 relative to compound 2.

[0113] In one embodiment, the target is immobilized on a solid support by any known immobilization technique. The solid support can be, for example, a water-insoluble matrix contained within a chromatography column or a membrane. The encoded library can be applied to a water-insoluble matrix contained within a chromatography column. The column is then washed to remove non-specific binders. Target-bound compounds can then be dissociated by changing the pH, salt concentration, organic solvent concentration, or other methods, such as competition with a known ligand to the target.

[0114] In another embodiment, the target is free in solution and is incubated with the encoded library. Compounds which bind to the target (also referred to herein as "ligands") are selectively isolated by a size separation step such as gel filtration or ultrafiltration. In one embodiment, the mixture of encoded compounds and the target biomolecule are passed through a size exclusion chromatography column (gel filtration), which separates any ligand-target complexes from the unbound compounds. The ligand-target complexes are transferred to a reverse-phase chromatography column, which dissociates the ligands from the target. The dissociated ligands are then analyzed by PCR amplification and sequence analysis of the encoding oligonucleotides. This approach is particularly advantageous in situations where immobilization of the target may result in a loss of activity.

[0115] Once single ligands are identified by the above-described process, various levels of analysis can be applied to yield structure-activity relationship information and to guide further optimization of the affinity, specificity and bioactivity of the ligand. For ligands derived from the same scaffold, three-dimensional molecular modeling can be employed to identify significant structural features common to the ligands, thereby generating families of small-molecule ligands that presumably bind at a common site on the target biomolecule.

[0116] A variety of screening approaches can be used to obtain ligands that possess high affinity for one target but significantly weaker affinity for another closely related target. One screening strategy is to identify ligands for both biomolecules in parallel experiments and to subsequently eliminate common ligands by a cross-referencing comparison. In this method, ligands for each biomolecule can be separately identified as disclosed above. This method is compatible with both immobilized target biomolecules and target biomolecules free in solution.

[0117] For immobilized target biomolecules, another strategy is to add a preselection step that eliminates all ligands that bind to the non-target biomolecule from the library. For example, a first biomolecule can be contacted with an encoded library as described above. Compounds which do not bind to the first biomolecule are then separated from any first biomolecule-ligand complexes which form. The second biomolecule is then contacted with the compounds which did not bind to the first biomolecule. Compounds which bind to the second biomolecule can be identified as described above and have significantly greater affinity for the second biomolecule than to the first biomolecule.

[0118] A ligand for a biomolecule of unknown function which is identified by the method disclosed above can also be used to determine the biological function of the biomolecule. This is advantageous because although new gene sequences continue to be identified, the functions of the proteins encoded by these sequences and the validity of these proteins as targets for new drug discovery and development are difficult to determine and represent perhaps the most significant obstacle to applying genomic information to the treatment of disease. Target-specific ligands obtained through the process described in this invention can be effectively employed in whole cell biological assays or in appropriate animal models to understand both the function of the target protein and the validity of the target protein for therapeutic intervention. This approach can also confirm that the target is specifically amenable to small molecule drug discovery.

[0119] In one embodiment, one or more compounds within a library of the invention are identified as ligands for a particular biomolecule. These compounds can then be assessed in an in vitro assay for the ability to bind to the biomolecule. Preferably, the functional moieties of the binding compounds are synthesized without the oligonucleotide tag or linker moiety, and these functional moieties are assessed for the ability to bind to the biomolecule.

[0120] The effect of the binding of the functional moieties to the biomolecule on the function of the biomolecule can also be assessed using in vitro cell-free or cell-based assays. For a biomolecule having a known function, the assay can include a comparison of the activity of the biomolecule in the presence and absence of the ligand, for example, by direct measurement of the activity, such as enzymatic activity, or by an indirect measure, such as a cellular function that is influenced by the biomolecule. If the biomolecule is of unknown function, a cell which expresses the biomolecule can be contacted with the ligand and the effect of the ligand on the viability, function, phenotype, and/or gene expression of the cell is assessed. The in vitro assay can be, for example, a cell death assay, a cell proliferation assay or a viral replication assay. For example, if the biomolecule is a protein expressed by a virus, a cell infected with the virus can be contacted with a ligand for the protein. The affect of the binding of the ligand to the protein on viral viability can then be assessed.

[0121] A ligand identified by the method of the invention can also be assessed in an in vivo model or in a human. For example, the ligand can be evaluated in an animal or organism which produces the biomolecule. Any resulting change in the health status (e.g., disease progression) of the animal or organism can be determined.

[0122] For a biomolecule, such as a protein or a nucleic acid molecule, of unknown function, the effect of a ligand which binds to the biomolecule on a cell or organism which produces the biomolecule can provide information regarding the biological function of the biomolecule. For example, the observation that a particular cellular process is inhibited in the presence of the ligand indicates that the process depends, at least in part, on the function of the biomolecule.

[0123] Ligands identified using the methods of the invention can also be used as affinity reagents for the biomolecule to which they bind. In one embodiment, such ligands are used to effect affinity purification of the biomolecule, for example, via chromatography of a solution comprising the biomolecule using a solid phase to which one or more such ligands are attached.

[0124] This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patents and published patent applications cited throughout this application, as well as the Figures and the Sequence Listing, are hereby incorporated in reference.

EXAMPLES

Example 1

Synthesis and Characterization of a Library on the Order of 10.sup.5 Members

[0125] The synthesis of a library comprising on the order of 10.sup.5 distinct members was accomplished using the following reagents:

Compound 1:

##STR00008##

[0126] Single letter codes for deoxyribonucleotides: A=adenosine C=cytidine G=guanosine T=thymidine

Building Block Precursors:

##STR00009## ##STR00010##

[0127] Oligonucleotide Tags:

TABLE-US-00001 [0128] Sequence Tag number 5'-PO.sub.4-GCAACGAAG (SEQ ID NO: 1) 1.1 ACCGTTGCT-PO.sub.3-5' (SEQ ID NO: 2) 5'-PO.sub.3-GCGTACAAG (SEQ ID NO: 3) 1.2 ACCGCATGT-PO.sub.3-5' (SEQ ID NO: 4) 5'-PO.sub.3-GCTCTGTAG (SEQ ID NO: 5) 1.3 ACCGAGACA-PO.sub.3-5' (SEQ ID NO: 6) 5'-PO.sub.3-GTGCCATAG (SEQ ID NO: 7) 1.4 ACCACGGTA-PO.sub.3-5' (SEQ ID NO: 8) 5'-PO.sub.3-GTTGACCAG (SEQ ID NO: 9) 1.5 ACCAACTGG-PO.sub.3-5' (SEQ ID NO: 10) 5'-PO.sub.3-CGACTTGAC (SEQ ID NO: 11) 1.6 CAAGTCGCA-PO.sub.3-5' (SEQ ID NO: 12) 5'-PO.sub.3-CGTAGTCAG (SEQ ID NO: 13) 1.7 ACGCATCAG-PO.sub.3-5' (SEQ ID NO: 14) 5'-PO.sub.3-CCAGCATAG (SEQ ID NO: 15) 1.8 ACGGTCGTA-PO.sub.3-5' (SEQ ID NO: 16) 5'-PO.sub.3-CCTACAGAG (SEQ ID NO: 17) 1.9 ACGGATGTC-PO.sub.3-5' (SEQ ID NO: 18) 5'-PO.sub.3-CTGAACGAG (SEQ ID NO: 19) 1.10 CGTTCAGCA-PO.sub.3-5' (SEQ ID NO: 20) 5'-PO.sub.3-CTCCAGTAG (SEQ ID NO: 21) 1.11 ACGAGGTCA-PO.sub.3-5' (SEQ ID NO: 22) 5'-PO.sub.3-TAGGTCCAG (SEQ ID NO: 23) 1.12 ACATCCAGG-PO.sub.3-5' (SEQ ID NO: 24) 5'-PO.sub.3-GCGTGTTGT (SEQ ID NO: 25) 2.1 TCCGCACAA-PO.sub.3-5' (SEQ ID NO: 26) 5'-PO.sub.3-GCTTGGAGT (SEQ ID NO: 27) 2.2 TCCGAACCT-PO.sub.3-5' (SEQ ID NO: 28) 5'-PO.sub.3-GTCAAGCGT (SEQ ID NO: 29) 2.3 TCCAGTTCG-PO.sub.3-5' (SEQ ID NO: 30) 5'-PO.sub.3-CAAGAGCGT (SEQ ID NO: 31) 2.4 TCGTTCTCG-PO.sub.3-5' (SEQ ID NO: 32) 5'-PO.sub.3-CAGTTCGGT (SEQ ID NO: 33) 2.5 TCGTCAAGC-PO.sub.3-5' (SEQ ID NO: 34) 5'-PO.sub.3-CGAAGGAGT (SEQ ID NO: 35) 2.6 TCGCTTCCT-PO.sub.3-5' (SEQ ID NO: 36) 5'-PO.sub.3-CGGTGTTGT (SEQ ID NO: 37) 2.7 TCGCCACAA-PO.sub.3-5' (SEQ ID NO: 38) 5'-PO.sub.3-CGTTGCTGT (SEQ ID NO: 39) 2.8 TCGCAACGA-PO.sub.3-5' (SEQ ID NO: 40) 5'-PO.sub.3-CCGATCTGT (SEQ ID NO: 41) 2.9 TCGGCTAGA-PO.sub.3-5' (SEQ ID NO: 42) 5'-PO.sub.3-CCTTCTCGT (SEQ ID NO: 43) 2.10 TCGGAAGAG-PO.sub.3-5' (SEQ ID NO: 44) 5'-PO.sub.3-TGAGTCCGT (SEQ ID NO: 45) 2.11 TCACTCAGG-PO.sub.3-5' (SEQ ID NO: 46) 5'-PO.sub.3-TGCTACGGT (SEQ ID NO: 47) 2.12 TCAGATTGC-PO.sub.3-5' (SEQ ID NO: 48) 5'-PO.sub.3-GTGCGTTGA (SEQ ID NO: 49) 3.1 CACACGCAA-PO.sub.3-5' (SEQ ID NO: 50) 5'-PO.sub.3-GTTGGCAGA (SEQ ID NO: 51) 3.2 CACAACCGT-PO.sub.3-5' (SEQ ID NO: 52) 5'-PO.sub.3-CCTGTAGGA (SEQ ID NO: 53) 3.3 CAGGACATC-PO.sub.3-5' (SEQ ID NO: 54) 5'-PO.sub.3-CTGCGTAGA (SEQ ID NO: 55) 3.4 CAGACGCAT-PO.sub.3-5' (SEQ ID NO: 56) 5'-PO.sub.3-CTTACGCGA (SEQ ID NO: 57) 3.5 CAGAATGCG-PO.sub.3-5' (SEQ ID NO: 58) 5'-PO.sub.3-TGGTCACGA (SEQ ID NO: 59) 3.6 CAACCAGTG-PO.sub.3-5' (SEQ ID NO: 60) 5'-PO.sub.3-TCAGAGCGA (SEQ ID NO: 61) 3.7 CAAGTCTCG-PO.sub.3-5' (SEQ ID NO: 62) 5'-PO.sub.3-TTGCTCGGA (SEQ ID NO: 63) 3.8 CAAACGAGC-PO.sub.3-5' (SEQ ID NO: 64) 5'-PO.sub.3-GCAGTTGGA (SEQ ID NO: 65) 3.9 CACGTCAAC-PO.sub.3-5' (SEQ ID NO: 66) 5'-PO.sub.3-GCCTGAAGA (SEQ ID NO: 67) 3.10 CACGGACTT-PO.sub.3-5' (SEQ ID NO: 68) 5'-PO.sub.3-GTAGCCAGA (SEQ ID NO: 69) 3.11 CACATCGGT-PO.sub.3-5' (SEQ ID NO: 70) 5'-PO.sub.3-GTCGCTTGA (SEQ ID NO: 71) 3.12 CACAGCGAA-PO.sub.3-5' (SEQ ID NO: 72) 5'-PO.sub.3-GCCTAAGTT (SEQ ID NO: 73) 4.1 CTCGGATTC-PO.sub.3-5' (SEQ ID NO: 74) 5'-PO.sub.3-GTAGTGCTT (SEQ ID NO: 75) 4.2 CTCATCACG-PO.sub.3-5' (SEQ ID NO: 76) 5'-PO.sub.3-GTCGAAGTT (SEQ ID NO: 77) 4.3 CTCAGCTTC-PO.sub.3-5' (SEQ ID NO: 78) 5'-PO.sub.3-GTTTCGGTT (SEQ ID NO: 79) 4.4 CTCAAAGCC-PO.sub.3-5' (SEQ ID NO: 80) 5'-PO.sub.3-CAGCGTTTT (SEQ ID NO: 81) 4.5 CTGTCGCAA-PO.sub.3-5' (SEQ ID NO: 82) 5'-PO.sub.3-CATACGCTT (SEQ ID NO: 83) 4.6 CTGTATGCG-P0.sub.3-5' (SEQ ID NO: 84) 5'-PO.sub.3-CGATCTGTT (SEQ ID NO: 85) 4.7 CTGCTAGAC-PO.sub.3-5' (SEQ ID NO: 86) 5'-PO.sub.3-CGCTTTGTT (SEQ ID NO: 87) 4.8 CTGCGAAAC-PO.sub.3-5' (SEQ ID NO: 88) 5'-PO.sub.3-CCACAGTTT (SEQ ID NO: 89) 4.9 CTGGTGTCA-PO.sub.3-5' (SEQ ID NO: 90) 5'-PO.sub.3-CCTGAAGTT (SEQ ID NO: 91) 4.10 CTGGACTTC-PO.sub.3-5' (SEQ ID NO: 92) 5'-PO.sub.3-CTGACGATT (SEQ ID NO: 93) 4.11 CTGACTGCT-PO.sub.3-5' (SEQ ID NO: 94) 5'-PO.sub.3-CTCCACTTT (SEQ ID NO: 95) 4.12 CTGAGGTGA-PO.sub.3-5' (SEQ ID NO: 96) 5'-PO.sub.3-ACCAGAGCC (SEQ ID NO: 97) 5.1 AATGGTCTC-PO.sub.3-5' (SEQ ID NO: 98) 5'-PO.sub.3-ATCCGCACC (SEQ ID NO: 99) 5.2 AATAGGCGT-PO.sub.3-5' (SEQ ID NO: 100) 5'-PO.sub.3-GACGACACC (SEQ ID NO: 101) 5.3 AACTGCTGT-PO.sub.3-5' (SEQ ID NO: 102) 5'-PO.sub.3-GGATGGACC (SEQ ID NO: 103) 5.4 AACCTACCT-PO.sub.3-5' (SEQ ID NO: 104) 5'-PO.sub.3-GCAGAAGCC (SEQ ID NO: 105) 5.5 AACGTCTTC-PO.sub.3-5' (SEQ ID NO: 106) 5'-PO.sub.3-GCCATGTCC (SEQ ID NO: 107) 5.6 AACGGTACA-PO.sub.3-5' (SEQ ID NO: 108) 5'-PO.sub.3-GTCTGCTCC (SEQ ID NO: 109) 5.7 AACAGACGA-PO.sub.3-5' (SEQ ID NO: 110) 5'-PO.sub.3-CGACAGACC (SEQ ID NO: 111) 5.8 AAGCTGTCT-PO.sub.3-5' (SEQ ID NO: 112) 5'-PO.sub.3-CGCTACTCC (SEQ ID NO: 113) 5.9 AAGCGATGA-PO.sub.3-5' (SEQ ID NO: 114) 5'-PO.sub.3-CCACAGACC (SEQ ID NO: 115) 5.10 AAGGTGTCT-PO.sub.3-5' (SEQ ID NO: 116) 5'-PO.sub.3-CCTCTCTCC (SEQ ID NO: 117) 5.11 AAGGAGAGA-PO.sub.3-5' (SEQ ID NO: 118) 5'-PO.sub.3-CTCGTAGCC (SEQ ID NO: 119) 5.12 AAGAGCATC-PO.sub.3-5' (SEQ ID NO: 120) l .times. ligase buffer: 50 mM Tris, pH 7.5; 10 mM dithiothreitol; 10 mM MgCl.sub.2; 2.5 mM ATP; 50 mM NaCl. 10 .times. ligase buffer: 500 mM Tris, pH 7.5; 100 mM dithiothreitol; 100 mM MgCl.sub.2; 25 mM ATP; 500 mM NaCl

Cycle 1

[0129] To each of twelve PCR tubes was added 50 .mu.L of a 1 mM solution of Compound 1 in water; 75 .mu.L of a 0.80 mM solution of one of Tags 1.1-1.12; 15 .mu.L 10.times. ligase buffer and 10 .mu.L deionized water. The tubes were heated to 95.degree. C. for 1 minute and then cooled to 16.degree. C. over 10 minutes. To each tube was added 5,000 units T4 DNA ligase (2.5 .mu.L of a 2,000,000 unit/mL solution (New England Biolabs, Cat. No. M0202)) in 50 .mu.l 1.times. ligase buffer and the resulting solutions were incubated at 16.degree. C. for 16 hours.

[0130] Following ligation, samples were transferred to 1.5 ml Eppendorf tubes and treated with 20 .mu.L 5 M aqueous NaCl and 500 .mu.L cold (-20.degree. C.) ethanol, and held at -20.degree. C. for 1 hour. Following centrifugation, the supernatant was removed and the pellet was washed with 70% aqueous ethanol at -20.degree. C. Each of the pellets was then dissolved in 150 .mu.L of 150 mM sodium borate buffer, pH 9.4.

[0131] Stock solutions comprising one each of building block precursors BB1 to BB12, N,N-diisopropylethanolamine and O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate, each at a concentration of 0.25 M, were prepared in DMF and stirred at room temperature for 20 minutes. The building block precursor solutions were added to each of the pellet solutions described above to provide a 10-fold excess of building block precursor relative to linker. The resulting solutions were stirred. An additional 10 equivalents of building block precursor was added to the reaction mixture after 20 minute, and another 10 equivalents after 40 minutes. The final concentration of DMF in the reaction mixture was 22%. The reaction solutions were then stirred overnight at 4.degree. C. The reaction progress was monitored by RP-HPLC using 50 mM aqueous tetraethylammonium acetate (pH=7.5) and acetonitrile, and a gradient of 2-46% acetonitrile over 14 min Reaction was stopped when .about.95% of starting material (linker) is acylated. Following acylation the reaction mixtures were pooled and lyophilized to dryness. The lyophilized material was then purified by HPLC, and the fractions corresponding to the library (acylated product) were pooled and lyophilized.

[0132] The library was dissolved in 2.5 ml of 0.01M sodium phosphate buffer (pH=8.2) and 0.1 ml of piperidine (4% v/v) was added to it. The addition of piperidine results in turbidity which does not dissolve on mixing. The reaction mixtures were stirred at room temperature for 50 minutes, and then the turbid solution was centrifuged (14,000 rpm), the supernatant was removed using a 200 .mu.l pipette, and the pellet was resuspended in 0.1 ml of water. The aqueous wash was combined with the supernatant and the pellet was discarded. The deprotected library was precipitated from solution by addition of excess ice-cold ethanol so as to bring the final concentration of ethanol in the reaction to 70% v/v. Centrifugation of the aqueous ethanol mixture gave a white pellet comprising the library. The pellet was washed once with cold 70% aq. ethanol. After removal of solvent the pellet was dried in air (.about.5 min) to remove traces of ethanol and then used in cycle 2. The tags and corresponding building block precursors used in Round 1 are set forth in Table 1, below.

TABLE-US-00002 TABLE 1 Building Block Precursor Tag BB1 1.11 BB2 1.6 BB3 1.2 BB4 1.8 BB5 1.1 BB6 1.10 BB7 1.12 BB8 1.5 BB9 1.4 BB10 1.3 BB11 1.7 BB12 1.9

Cycles 2-5

[0133] For each of these cycles, the combined solution resulting from the previous cycle was divided into 12 equal aliquots of 50 ul each and placed in PCR tubes. To each tube was added a solution comprising a different tag, and ligation, purification and acylation were performed as described for Cycle 1, except that for Cycles 3-5, the HPLC purification step described for Cycle 1 was omitted. The correspondence between tags and building block precursors for Cycles 2-5 is presented in Table 2.

[0134] The products of Cycle 5 were ligated with the closing primer shown below, using the method described above for ligation of tags.

TABLE-US-00003 5'-PO.sub.3-GGCACATTGATTTGGGAGTCA (SEQ ID NO: 893) GTGTAACTAAACCCTCAGT-PO.sub.3-5' (SEQ ID NO: 894)

TABLE-US-00004 TABLE 2 Building Block Cycle 2 Cycle 3 Cycle 4 Cycle 5 Precursor Tag Tag Tag Tag BB1 2.7 3.7 4.7 5.7 BB2 2.8 3.8 4.8 5.8 BB3 2.2 3.2 4.2 5.2 BB4 2.10 3.10 4.10 5.10 BB5 2.1 3.1 4.1 5.1 BB6 2.12 3.12 4.12 5.12 BB7 2.5 3.5 4.5 5.5 BB8 2.6 3.6 4.6 5.6 BB9 2.4 3.4 4.4 5.4 BB10 2.3 3.3 4.3 5.3 BB11 2.9 3.9 4.9 5.9 BB12 2.11 3.11 4.11 5.11

Results:

[0135] The synthetic procedure described above has the capability of producing a library comprising 12.sup.5 (about 249,000) different structures. The synthesis of the library was monitored via gel electrophoresis of the product of each cycle. The results of each of the five cycles and the final library following ligation of the closing primer are illustrated in FIG. 7. The compound labeled "head piece" is Compound 1. The figure shows that each cycle results in the expected molecular weight increase and that the products of each cycle are substantially homogeneous with regard to molecular weight.

Example 2

Synthesis and Characterization of a Library on the Order of 10.sup.8 Members

[0136] The synthesis of a library comprising on the order of 10.sup.8 distinct members was accomplished using the following reagents:

Compound 2:

##STR00011##

[0137] Single letter codes for deoxyribonucleotides: A=adenosine C=cytidine G=guanosine T=thymidine

Building Block Precursors:

##STR00012## ##STR00013## ##STR00014## ##STR00015## ##STR00016## ##STR00017## ##STR00018## ##STR00019## ##STR00020## ##STR00021## ##STR00022## ##STR00023## ##STR00024## ##STR00025## ##STR00026## ##STR00027##

TABLE-US-00005 [0138] TABLE 3 Oligonucleotide tags used in cycle 1: Tag Number Top Strand Sequence Bottom Strand Sequence 1.1 5'-PO3-AAATCGATGTGGTCACTCAG 5'-PO3-GAGTGACCACATCGATTTGG (SEQ ID NO: 121) (SEQ ID NO: 122) 1.2 5'-PO3-AAATCGATGTGGACTAGGAG 5'-PO3-CCTAGTCCACATCGATTTGG (SEQ ID NO: 123) (SEQ ID NO: 124) 1.3 5'-PO3-AAATCGATGTGCCGTATGAG 5'-PO3-CATACGGCACATCGATTTGG (SEQ ID NO: 125) (SEQ ID NO: 126) 1.4 5'-PO3-AAATCGATGTGCTGAAGGAG 5'-PO3-CCTTCAGCACATCGATTTGG (SEQ ID NO: 127) (SEQ ID NO: 128) 1.5 5'-PO3-AAATCGATGTGGACTAGCAG 5'-PO3-GCTAGTCCACATCGATTTGG (SEQ ID NO: 129) (SEQ ID NO: 130) 1.6 5'-PO3-AAATCGATGTGCGCTAAGAG 5'-PO3-CTTAGCGCACATCGATTTGG (SEQ ID NO: 131) (SEQ ID NO: 132) 1.7 5'-PO3-AAATCGATGTGAGCCGAGAG 5'-PO3-CTCGGCTCACATCGATTTGG (SEQ ID NO: 133) (SEQ ID NO: 134) 1.8 5'-PO3-AAATCGATGTGCCGTATCAG 5'-PO3-GATACGGCACATCGATTTGG (SEQ ID NO: 135) (SEQ ID NO: 136) 1.9 5'-PO3-AAATCGATGTGCTGAAGCAG 5'-PO3-GCTTCAGCACATCGATTTGG (SEQ ID NO: 137) (SEQ ID NO: 138) 1.10 5'-PO3-AAATCGATGTGTGCGAGTAG 5'-PO3-ACTCGCACACATCGATTTGG (SEQ ID NO: 139) (SEQ ID NO: 140) 1.11 5'-PO3-AAATCGATGTGTTTGGCGAG 5'-PO3-CGCCAAACACATCGATTTGG (SEQ ID NO: 141) (SEQ ID NO: 142) 1.12 5'-PO3-AAATCGATGTGCGCTAACAG 5'-PO3-GTTAGCGCACATCGATTTGG (SEQ ID NO: 143) (SEQ ID NO: 144) 1.13 5'-PO3-AAATCGATGTGAGCCGACAG 5'-PO3-GTCGGCTCACATCGATTTGG (SEQ ID NO: 145) (SEQ ID NO: 146) 1.14 5'-PO3-AAATCGATGTGAGCCGAAAG 5'-PO3-TTCGGCTCACATCGATTTGG (SEQ ID NO: 147) (SEQ ID NO: 148) 1.15 5'-PO3-AAATCGATGTGTCGGTAGAG 5'-PO3-CTACCGACACATCGATTTGG (SEQ ID NO: 149) (SEQ ID NO: 150) 1.16 5'-PO3-AAATCGATGTGGTTGCCGAG 5'-PO3-CGGCAACCACATCGATTTGG (SEQ ID NO: 151) (SEQ ID NO: 152) 1.17 5'-PO3-AAATCGATGTGAGTGCGTAG 5'-PO3-ACGCACTCACATCGATTTGG (SEQ ID NO: 153) (SEQ ID NO: 154) 1.18 5'-PO3-AAATCGATGTGGTTGCCAAG 5'-PO3-TGGCAACCACATCGATTTGG (SEQ ID NO: 155) (SEQ ID NO: 156) 1.19 5'-PO3-AAATCGATGTGTGCGAGGAG 5'-PO3-CCTCGCACACATCGATTTGG (SEQ ID NO: 157) (SEQ ID NO: 158) 1.20 5'-PO3-AAATCGATGTGGAACACGAG 5'-PO3-CGTGTTCCACATCGATTTGG (SEQ ID NO: 159) (SEQ ID NO: 160) 1.21 5'-PO3-AAATCGATGTGCTTGTCGAG 5'-PO3-CGACAAGCACATCGATTTGG (SEQ ID NO: 161) (SEQ ID NO: 162) 1.22 5'-PO3-AAATCGATGTGTTCCGGTAG 5'-PO3-A0CCGGAACACATCGATTTGG (SEQ ID NO: 163) (SEQ ID NO: 164) 1.23 5'-PO3-AAATCGATGTGTGCGAGCAG 5'-PO3-GCTCGCACACATCGATTTGG (SEQ ID NO: 165) (SEQ ID NO: 166) 1.24 5'-PO3-AAATCGATGTGGTCAGGTAG 5'-PO3-ACCTGACCACATCGATTTGG (SEQ ID NO: 167) (SEQ ID NO: 168) 1.25 5'-PO3-AAATCGATGTGGCCTGTTAG 5'-PO3-AACAGGCCACATCGATTTGG (SEQ ID NO: 169) (SEQ ID NO: 170) 1.26 5'-PO3-AAATCGATGTGGAACACCAG 5'-PO3-GGTGTTCCACATCGATTTGG (SEQ ID NO: 171) (SEQ ID NO: 172) 1.27 5'-PO3-AAATCGATGTGCTTGTCCAG 5'-PO3-GGACAAGCACATCGATTTGG (SEQ ID NO: 173) (SEQ ID NO: 174) 1.28 5'-PO3-AAATCGATGTGTGCGAGAAG 5'-PO3-TCTCGCACACATCGATTTGG (SEQ ID NO: 175) (SEQ ID NO: 176) 1.29 5'-PO3-AAATCGATGTGAGTGCGGAG 5'-PO3-CCGCACTCACATCGATTTGG (SEQ ID NO: 177) (SEQ ID NO: 178) 1.30 5'-PO3-AAATCGATGTGTTGTCCGAG 5'-PO3-CGGACAACACATCGATTTGG (SEQ ID NO: 179) (SEQ ID NO: 180) 1.31 5'-PO3-AAATCGATGTGTGGAACGAG 5'-PO3-CGTTCCACACATCGATTTGG (SEQ ID NO: 181) (SEQ ID NO: 182) 1.32 5'-PO3-AAATCGATGTGAGTGCGAAG 5'-PO3-TCGCACTCACATCGATTTGG (SEQ ID NO: 183) (SEQ ID NO: 184) 1.33 5'-PO3-AAATCGATGTGTGGAACCAG 5'-PO3-GGTTCCACACATCGATTTGG (SEQ ID NO: 185) (SEQ ID NO: 186) 1.34 5'-PO3-AAATCGATGTGTTAGGCGAG 5'-PO3-CGCCTAACACATCGATTTGG (SEQ ID NO: 187) (SEQ ID NO: 188) 1.35 5'-PO3-AAATCGATGTGGCCTGTGAG 5'-PO3-CACAGGCCACATCGATTTGG (SEQ ID NO: 189) (SEQ ID NO: 190) 1.36 5'-PO3-AAATCGATGTGCTCCTGTAG 5'-PO3-ACAGGAGCACATCGATTTGG (SEQ ID NO: 191) (SEQ ID NO: 192) 1.37 5'-PO3-AAATCGATGTGGTCAGGCAG 5'-PO3-GCCTGACCACATCGATTTGG (SEQ ID NO: 193) (SEQ ID NO: 194) 1.38 5'-PO3-AAATCGATGTGGTCAGGAAG 5'-PO3-TCCTGACCACATCGATTTGG (SEQ ID NO: 195) (SEQ ID NO: 196) 1.39 5'-PO3-AAATCGATGTGGTAGCCGAG 5'-PO3-CGGCTACCACATCGATTTGG (SEQ ID NO: 197) (SEQ ID NO: 198) 1.40 5'-PO3-AAATCGATGTGGCCTGTAAG 5'-PO3-TACAGGCCACATCGATTTGG (SEQ ID NO: 199) (SEQ ID NO: 200) 1.41 5'-PO3-AAATCGATGTGCTTTCGGAG 5'-PO3-CCGAAAGCACATCGATTTGG (SEQ ID NO: 201) (SEQ ID NO: 202) 1.42 5'-PO3-AAATCGATGTGCGTAAGGAG 5'-PO3-CCTTACGCACATCGATTTGG (SEQ ID NO: 203) (SEQ ID NO: 204) 1.43 5'-PO3-AAATCGATGTGAGAGCGTAG 5'-PO3-ACGCTCTCACATCGATTTGG (SEQ ID NO: 205) (SEQ ID NO: 206) 1.44 5'-PO3-AAATCGATGTGGACGGCAAG 5'-PO3-TGCCGTCCACATCGATTTGG (SEQ ID NO: 207) (SEQ ID NO: 208) 1.45 5'-PO3-AAATCGATGTGCTTTCGCAG 5'-PO3-GCGAAAGCACATCGATTTGG (SEQ ID NO: 209) (SEQ ID NO: 210) 1.46 5'-PO3-AAATCGATGTGCGTAAGCAG 5'-PO3-GCTTACGCACATCGATTTGG (SEQ ID NO: 211) (SEQ ID NO: 212) 1.47 5'-PO3-AAATCGATGTGGCTATGGAG 5'-PO3-CCATAGCCACATCGATTTGG (SEQ ID NO: 213) (SEQ ID NO: 214) 1.48 5'-PO3-AAATCGATGTGACTCTGGAG 5'-PO3-CCAGAGTCACATCGATTTGG (SEQ ID NO: 215) (SEQ ID NO: 216) 1.49 5'-PO3-AAATCGATGTGCTGGAAAG 5'-PO3-TTCCAGCACATCGATTTGG (SEQ ID NO: 217) (SEQ ID NO: 218) 1.50 5'-PO3-AAATCGATGTGCCGAAGTAG 5'-PO3-ACTTCGGCACATCGATTTGG (SEQ ID NO: 219) (SEQ ID NO: 220) 1.51 5'-PO3-AAATCGATGTGCTCCTGAAG 5'-PO3-TCAGGAGCACATCGATTTGG (SEQ ID NO: 221) (SEQ ID NO: 222) 1.52 5'-PO3-AAATCGATGTGTCCAGTCAG 5'-PO3-GACTGGACACATCGATTTGG (SEQ ID NO: 223) (SEQ ID NO: 224) 1.53 5'-PO3-AAATCGATGTGAGAGCGGAG 5'-PO3-CCGCTCTCACATCGATTTGG (SEQ ID NO: 225) (SEQ ID NO: 226) 1.54 5'-PO3-AAATCGATGTGAGAGCGAAG 5'-PO3-TCGCTCTCACATCGATTTGG (SEQ ID NO: 227) (SEQ ID NO: 228) 1.55 5'-PO3-AAATCGATGTGCCGAAGGAG 5'-PO3-CCTTCGGCACATCGATTTGG (SEQ ID NO: 229) (SEQ ID NO: 230) 1.56 5'-PO3-AAATCGATGTGCCGAAGCAG 5'-PO3-GCTTCGGCACATCGATTTGG (SEQ ID NO: 231) (SEQ ID NO: 232) 1.57 5'-PO3-AAATCGATGTGTGTTCCGAG 5'-PO3-CGGAACACACATCGATTTGG (SEQ ID NO: 233) (SEQ ID NO: 234) 1.58 5'-PO3-AAATCGATGTGTCTGGCGAG 5'-PO3-CGCCAGACACATCGATTTGG (SEQ ID NO: 235) (SEQ ID NO: 236) 1.59 5'-PO3-AAATCGATGTGCTATCGGAG 5'-PO3-CCGATAGCACATCGATTTGG (SEQ ID NO: 237) (SEQ ID NO: 238) 1.60 5'-PO3-AAATCGATGTGCGAAAGGAG 5'-PO3-CCTTTCGCACATCGATTTGG (SEQ ID NO: 239) (SEQ ID NO: 240) 1.61 5'-PO3-AAATCGATGTGCCGAAGAAG 5'-PO3-TCTTCGGCACATCGATTTGG (SEQ ID NO: 241) (SEQ ID NO: 242) 1.62 5'-PO3-AAATCGATGTGGTTGCAGAG 5'-PO3-CTGCAACCACATCGATTTGG (SEQ ID NO: 243) (SEQ ID NO: 244) 1.63 5'-PO3-AAATCGATGTGGATGGTGAG 5'-PO3-CACCATCCACATCGATTTGG (SEQ ID NO: 245) (SEQ ID NO: 246) 1.64 5'-PO3-AAATCGATGTGCTATCGCAG 5'-PO3-GCGATAGCACATCGATTTGG (SEQ ID NO: 247) (SEQ ID NO: 248) 1.65 5'-PO3-AAATCGATGTGCGAAAGCAG 5'-PO3-GCTTTCGCACATCGATTTGG (SEQ ID NO: 249) (SEQ ID NO: 250) 1.66 5'-PO3-AAATCGATGTGACACTGGAG 5'-PO3-CCAGTGTCACATCGATTTGG (SEQ ID NO: 251) (SEQ ID NO: 252) 1.67 5'-PO3-AAATCGATGTGTCTGGCAAG 5'-PO3-TGCCAGACACATCGATTTGG (SEQ ID NO: 253) (SEQ ID NO: 254) 1.68 5'-PO3-AAATCGATGTGGATGGTCAG 5'-PO3-GACCATCCACATCGATTTGG (SEQ ID NO: 255) (SEQ ID NO: 256) 1.69 5'-PO3-AAATCGATGTGGTTGCACAG 5'-PO3-GTGCAACCACATCGATTTGG (SEQ ID NO: 257) (SEQ ID NO: 258) 1.70 5'-PO3-AAATCGATGTGGGCATCGAG 5'-PO3-CGATGCCCCATCCGATTTGG (SEQ ID NO: 259) (SEQ ID NO: 260) 1.71 5'-PO3-AAATCGATGTGTGCCTCCAG 5'-PO3-GGAGGCACACATCGATTTGG (SEQ ID NO: 261) (SEQ ID NO: 262) 1.72 5'-PO3-AAATCGATGTGTGCCTCAAG 5'-PO3-TGAGGCACACATCGATTTGG (SEQ ID NO: 263) (SEQ ID NO: 264) 1.73 5'-PO3-AAATCGATGTGGGCATCCAG 5'-PO3-GGATGCCCACATCGATTTGG (SEQ ID NO: 265) (SEQ ID NO: 266) 1.74 5'-PO3-AAATCGATGTGGGCATCAAG 5'-PO3-TGATGCCCA CAT CGA TTT GG (SEQ ID NO: 267) (SEQ ID NO: 268) 1.75 5'-PO3-AAATCGATGTGCCTGTCGAG 5'-PO3-CGA CAG GCA CAT CGA TTT GG (SEQ ID NO: 269) (SEQ ID NO: 270) 1.76 5'-PO3-AAATCGATGTGGACGGATAG 5'-PO3-ATC CGT CCA CAT CGA TTT GG (SEQ ID NO: 271) (SEQ ID NO: 272) 1.77 5'-PO3-AAATCGATGTGCCTGTCCAG 5'-PO3-GGA CAG GCA CAT CGA TTT GG (SEQ ID NO: 273) (SEQ ID NO: 274) 1.78 5'-PO3-AAATCGATGTGAAGCACGAG 5'-PO3-CGT GCT TCA CAT CGA TTT GG (SEQ ID NO: 275) (SEQ ID NO: 276) 1.79 5'-PO3-AAATCGATGTGCCTGTCAAG 5'-PO3-TGA CAG GCA CAT CGA TTT GG (SEQ ID NO: 277) (SEQ ID NO: 278) 1.80 5'-PO3-AAATCGATGTGAAGCACCAG 5'-PO3-GGT GCT TCA CAT CGA TTT GG (SEQ ID NO: 279) (SEQ ID NO: 280) 1.81 5'-PO3-AAATCGATGTGCCTTCGTAG 5'-PO3-ACG AAG GCA CAT CGA TTT GG (SEQ ID NO: 281) (SEQ ID NO: 282) 1.82 5'-PO3-AAATCGATGTGTCGTCCGAG 5'-PO3-CGG ACG ACA CAT CGA TTT GG (SEQ ID NO: 283) (SEQ ID NO: 284)

1.83 5'-PO3-AAATCGATGTGGAGTCTGAG 5'-PO3-CAG ACT CCA CAT CGA TTT GG (SEQ ID NO: 285) (SEQ ID NO: 286) 1.84 5'-PO3-AAATCGATGTGTGATCCGAG 5'-PO3-CGG ATC ACA CAT CGA TTT GG (SEQ ID NO: 287) (SEQ ID NO: 288) 1.85 5'-PO3-AAATCGATGTGTCAGGCGAG 5'-PO3-CGC CTG ACA CAT CGA TTT GG (SEQ ID NO: 289) (SEQ ID NO: 290) 1.86 5'-PO3-AAATCGATGTGTCGTCCAAG 5'-PO3-TGG ACG ACA CAT CGA TTT GG (SEQ ID NO: 291) (SEQ ID NO: 292) 1.87 5'-PO3-AAATCGATGTGGACGGAGAG 5'-PO3-CTC CGT CCA CAT CGA TTT GG (SEQ ID NO: 293) (SEQ ID NO: 294) 1.88 5'-PO3-AAATCGATGTGGTAGCAGAG 5'-PO3-CTG CTA CCA CAT CGA TTT GG (SEQ ID NO: 295) (SEQ ID NO: 296) 1.89 5'-PO3-AAATCGATGTGGCTGTGTAG 5'-PO3-ACACAGCCACATCGATTTGG (SEQ ID NO: 297) (SEQ ID NO: 298) 1.90 5'-PO3-AAATCGATGTGGACGGACAG 5'-PO3-GTC CGT CCA CAT CGA TTT GG (SEQ ID NO: 299) (SEQ ID NO: 300) 1.91 5'-PO3-AAATCGATGTGTCAGGCAAG 5'-PO3-TGC CTG ACA CAT CGA TTT GG (SEQ ID NO: 301) (SEQ ID NO: 302) 1.92 5'-PO3-AAATCGATGTGGCTCGAAAG 5'-PO3-TTCGAGCCACATCGATTTGG (SEQ ID NO: 303) (SEQ ID NO: 304) 1.93 5'-PO3-AAATCGATGTGCCTTCGGAG 5'-PO3-CCG AAG GCA CAT CGA TTT GG (SEQ ID NO: 305) (SEQ ID NO: 306) 1.94 5'-PO3-AAATCGATGTGGTAGCACAG 5'-PO3-GTG CTA CCA CAT CGA TTT GG (SEQ ID NO: 307) (SEQ ID NO: 308) 1.95 5'-PO3-AAATCGATGTGGAAGGTCAG 5'-PO3-GAC CTT CCA CAT CGA TTT GG (SEQ ID NO: 309) (SEQ ID NO: 310) 1.96 5'-PO3-AAATCGATGTGGTGCTGTAG 5'-PO3-ACA GCA CCA CAT CGA TTT GG (SEQ ID NO: 311) (SEQ ID NO: 312)

TABLE-US-00006 TABLE 4 Oligonucleotide tags used in cycle 2: Tag Top Bottom Number strand sequence strand sequence 2.1 5'-PO3-GTT GCC TGT 5'-PO3-AGG CAA CCT (SEQ ID NO: 313) (SEQ ID NO: 314) 2.2 5'-PO3-CAG GAC GGT 5'-PO3-CGT CCT GCT (SEQ ID NO: 315) (SEQ ID NO: 316) 2.3 5'-PO3-AGA CGT GGT 5'-PO3-CAC GTC TCT (SEQ ID NO: 317) (SEQ ID NO: 318) 2.4 5'-PO3-CAG GAC CGT 5'-PO3-GGT CCT GCT (SEQ ID NO: 319) (SEQ ID NO: 320) 2.5 5'-PO3-CAG GAC AGT 5'-PO3-TGT CCT GCT (SEQ ID NO: 321) (SEQ ID NO: 322) 2.6 5'-PO3-CAC TCT GGT 5'-PO3-CAG AGT GCT (SEQ ID NO: 323) (SEQ ID NO: 324) 2.7 5'-PO3-GAC GGC TGT 5'-PO3-AGC CGT CCT (SEQ ID NO: 325) (SEQ ID NO: 326) 2.8 5'-PO3-CAC TCT CGT 5'-PO3-GAG AGT GCT (SEQ ID NO: 327) (SEQ ID NO: 328) 2.9 5'-PO3-GTA GCC TGT 5'-PO3-AGG CTA CCT (SEQ ID NO: 329) (SEQ ID NO: 330) 2.10 5'-PO3-GCC ACT TGT 5'-PO3-AAG TGG CCT (SEQ ID NO: 331) (SEQ ID NO: 332) 2.11 5'-PO3-CAT CGC TGT 5'-PO3-AGC GAT GCT (SEQ ID NO: 333) (SEQ ID NO: 334) 2.12 5'-PO3-CAC TGG TGT 5'-PO3-ACC AGT GCT (SEQ ID NO: 335) (SEQ ID NO: 336) 2.13 5'-PO3-GCC ACT GGT 5'-PO3-CAG TGG CCT (SEQ ID NO: 337) (SEQ ID NO: 338) 2.14 5'-PO3-TCT GGC TGT 5'-PO3-AGC CAG ACT (SEQ ID NO: 339) (SEQ ID NO: 340) 2.15 5'-PO3-GCC ACT CGT 5'-PO3-GAG TGG CCT (SEQ ID NO: 341) (SEQ ID NO: 342) 2.16 5'-PO3-TGC CTC TGT 5'-PO3-AGA GGC ACT (SEQ ID NO: 343) (SEQ ID NO: 344) 2.17 5'-PO3-CAT CGC AGT 5'-PO3-TGC GAT GCT (SEQ ID NO: 345) (SEQ ID NO: 346) 2.18 5'-PO3-CAG GAA GGT 5'-PO3-CTT CCT GCT (SEQ ID NO: 347) (SEQ ID NO: 348) 2.19 5'-PO3-GGC ATC TGT 5'-PO3-AGA TGC CCT (SEQ ID NO: 349) (SEQ ID NO: 350) 2.20 5'-PO3-CGG TGC TGT 5'-PO3-AGC ACC GCT (SEQ ID NO: 351) (SEQ ID NO: 352) 2.21 5'-PO3-CAC TGG CGT 5'-PO3-GCC AGT GCT (SEQ ID NO: 353) (SEQ ID NO: 354) 2.22 5'-PO3-TCTCCTCGT 5'-PO3-GAGGAGACT (SEQ ID NO: 355) (SEQ ID NO: 356) 2.23 5'-PO3-CCT GTC TGT 5'-PO3-AGA CAG GCT (SEQ ID NO: 357) (SEQ ID NO: 358) 2.24 5'-PO3-CAA CGC TGT 5'-PO3-AGC GTT GCT (SEQ ID NO: 359) (SEQ ID NO: 360) 2.25 5'-PO3-TGC CTC GGT 5'-PO3-CGA GGC ACT (SEQ ID NO: 361) (SEQ ID NO: 362) 2.26 5'-PO3-ACA CTG CGT 5'-PO3-GCA GTG TCT (SEQ ID NO: 363) (SEQ ID NO: 364) 2.27 5'-PO3-TCG TCC TGT 5'-PO3-AGG ACG ACT (SEQ ID NO: 365) (SEQ ID NO: 366) 2.28 5'-PO3-GCT GCC AGT 5'-PO3-TGG CAG CCT (SEQ ID NO: 367) (SEQ ID NO: 368) 2.29 5'-PO3-TCA GGC TGT 5'-PO3-AGC CTG ACT (SEQ ID NO: 369) (SEQ ID NO: 370) 2.30 5'-PO3-GCC AGG TGT 5'-PO3-ACC TGG CCT (SEQ ID NO: 371) (SEQ ID NO: 372) 2.31 5'-PO3-CGG ACC TGT 5'-PO3-AGG TCC GCT (SEQ ID NO: 373) (SEQ ID NO: 374) 2.32 5'-PO3-CAA CGC AGT 5'-PO3-TGC GTT GCT (SEQ ID NO: 375) (SEQ ID NO: 376) 2.33 5'-PO3-CAC ACG AGT 5'-PO3-TCG TGT GCT (SEQ ID NO: 377) (SEQ ID NO: 378) 2.34 5'-PO3-ATG GCC TGT 5'-PO3-AGG CCA TCT (SEQ ID NO: 379) (SEQ ID NO: 380) 2.35 5'-PO3-CCA GTC TGT 5'-PO3-AGA CTG GCT (SEQ ID NO: 381) (SEQ ID NO: 382) 2.36 5'-PO3-GCC AGG AGT 5'-PO3-TCC TGG CCT (SEQ ID NO: 383) (SEQ ID NO: 384) 2.37 5'-PO3-CGG ACC AGT 5'-PO3-TGG TCC GCT (SEQ ID NO: 385) (SEQ ID NO: 386) 2.38 5'-PO3-CCT TCG CGT 5'-PO3-GCG AAG GCT (SEQ ID NO: 387) (SEQ ID NO: 388) 2.39 5'-PO3-GCA GCC AGT 5'-PO3-TGG CTG CCT (SEQ ID NO: 389) (SEQ ID NO: 390) 2.40 5'-PO3-CCA GTC GGT 5'-PO3-CGA CTG GCT (SEQ ID NO: 391) (SEQ ID NO: 392) 2.41 5'-PO3-ACT GAG CGT 5'-PO3-GCT CAG TCT (SEQ ID NO: 393) (SEQ ID NO: 394) 2.42 5'-PO3-CCA GTC CGT 5'-PO3-GGA CTG GCT (SEQ ID NO: 395) (SEQ ID NO: 396) 2.43 5'-PO3-CCA GTC AGT 5'-PO3-TGA CTG GCT (SEQ ID NO: 397) (SEQ ID NO: 398) 2.44 5'-PO3-CAT CGA GGT 5'-PO3-CTC GAT GCT (SEQ ID NO: 399) (SEQ ID NO: 400) 2.45 5'-PO3-CCA TCG TGT 5'-PO3-ACG ATG GCT (SEQ ID NO: 401) (SEQ ID NO: 402) 2.46 5'-PO3-GTG CTG CGT 5'-PO3-GCA GCA CCT (SEQ ID NO: 403) (SEQ ID NO: 404) 2.47 5'-PO3-GAC TAC GGT 5'-PO3-CGT AGT CCT (SEQ ID NO: 405) (SEQ ID NO: 406) 2.48 5'-PO3-GTG CTG AGT 5'-PO3-TCA GCA CCT (SEQ ID NO: 407) (SEQ ID NO: 408) 2.49 5'-PO3-GCTGCATGT 5'-PO3-ATGCAGCCT (SEQ ID NO: 409) (SEQ ID NO: 410) 2.50 5'-PO3-GAGTGGTGT 5'-PO3-ACCACTCCT (SEQ ID NO: 411) (SEQ ID NO: 412) 2.51 5'-PO3-GACTACCGT 5'-PO3-GGTAGTCCT (SEQ ID NO: 413) (SEQ ID NO: 414) 2.52 5'-PO3-CGGTGATGT 5'-PO3-ATCACCGCT (SEQ ID NO: 415) (SEQ ID NO: 416) 2.53 5'-PO3-TGCGACTGT 5'-PO3-AGTCGCACT (SEQ ID NO: 417) (SEQ ID NO: 418) 2.54 5'-PO3-TCTGGAGGT 5'-PO3-CTCCAGACT (SEQ ID NO: 419) (SEQ ID NO: 420) 2.55 5'-PO3-AGCACTGGT 5'-PO3-CAGTGCTCT (SEQ ID NO: 421) (SEQ ID NO: 422) 2.56 5'-PO3-TCGCTTGGT 5'-PO3-CAAGCGACT (SEQ ID NO: 423) (SEQ ID NO: 424) 2.57 5'-PO3-AGCACTCGT 5'-PO3-GAGTGCTCT (SEQ ID NO: 425) (SEQ ID NO: 426) 2.58 5'-PO3-GCGATTGGT 5'-PO3-CAATCGCCT (SEQ ID NO: 427) (SEQ ID NO: 428) 2.59 5'-PO3-CCATCGCGT 5'-PO3-GCGATGGCT (SEQ ID NO: 429) (SEQ ID NO: 430) 2.60 5'-PO3-TCGCTTCGT 5'-PO3-GAAGCGACT (SEQ ID NO: 431) (SEQ ID NO: 432) 2.61 5'-PO3-AGTGCCTGT 5'-PO3-AGGCACTCT (SEQ ID NO: 433) (SEQ ID NO: 434) 2.62 5'-PO3-GGCATAGGT 5'-PO3-CTATGCCCT (SEQ ID NO: 435) (SEQ ID NO: 436) 2.63 5'-PO3-GCGATTCGT 5'-PO3-GAATCGCCT (SEQ ID NO: 437) (SEQ ID NO: 438) 2.64 5'-PO3-TGCGACGGT 5'-PO3-CGTCGCACT (SEQ ID NO: 439) (SEQ ID NO: 440) 2.65 5'-PO3-GAGTGGCGT 5'-PO3-GCCACTCCT (SEQ ID NO: 441) (SEQ ID NO: 442) 2.66 5'-PO3-CGGTGAGGT 5'-PO3-CTCACCGCT (SEQ ID NO: 443) (SEQ ID NO: 444) 2.67 5'-PO3-GCTGCAAGT 5'-PO3-TTGCAGCCT (SEQ ID NO: 445) (SEQ ID NO: 446) 2.68 5'-PO3-TTCCGCTGT 5'-PO3-AGCGGAACT (SEQ ID NO: 447) (SEQ ID NO: 448) 2.69 5'-PO3-GAGTGGAGT 5'-PO3-TCCACTCCT (SEQ ID NO: 449) (SEQ ID NO: 450) 2.70 5'-PO3-ACAGAGCGT 5'-PO3-GCTCTGTCT (SEQ ID NO: 451) (SEQ ID NO: 452) 2.71 5'-PO3-TGCGACCGT 5'-PO3-GGTCGCACT (SEQ ID NO: 453) (SEQ ID NO: 454) 2.72 5'-PO3-CCTGTAGGT 5'-PO3-CTACAGGCT (SEQ ID NO: 455) (SEQ ID NO: 456) 2.73 5'-PO3-TAGCCGTGT 5'-PO3-ACGGCTACT (SEQ ID NO: 457) (SEQ ID NO: 458) 2.74 5'-PO3-TGCGACAGT 5'-PO3-TGTCGCACT (SEQ ID NO: 459) (SEQ ID NO: 460) 2.75 5'-PO3-GGTCTGTGT 5'-PO3-ACAGACCCT (SEQ ID NO: 461) (SEQ ID NO: 462) 2.76 5'-PO3-CGGTGAAGT 5'-PO3-TTCACCGCT (SEQ ID NO: 463) (SEQ ID NO: 464) 2.77 5'-PO3-CAACGAGGT 5'-PO3-CTCGTTGCT (SEQ ID NO: 465) (SEQ ID NO: 466) 2.78 5'-PO3-GCAGCATGT 5'-PO3-ATGCTGCCT (SEQ ID NO: 467) (SEQ ID NO: 468) 2.79 5'-PO3-TCGTCAGGT 5'-PO3-CTGACGACT (SEQ ID NO: 469) (SEQ ID NO: 470) 2.80 5'-PO3-AGTGCCAGT 5'-PO3-TGGCACTCT (SEQ ID NO: 471) (SEQ ID NO: 472) 2.81 5'-PO3-TAGAGGCGT 5'-PO3-GCCTCTACT (SEQ ID NO: 473) (SEQ ID NO: 474) 2.82 5'-PO3-GTCAGCGGT 5'-PO3-CGCTGACCT

(SEQ ID NO: 475) (SEQ ID NO: 476) 2.83 5'-PO3-TCAGGAGGT 5'-PO3-CTCCTGACT (SEQ ID NO: 477) (SEQ ID NO: 478) 2.84 5'-PO3-AGCAGGTGT 5'-PO3-ACCTGCTCT (SEQ ID NO: 479) (SEQ ID NO: 480) 2.85 5'-PO3-TTCCGCAGT 5'-PO3-TGCGGAACT (SEQ ID NO: 481) (SEQ ID NO: 482) 2.86 5'-PO3-GTCAGCCGT 5'-PO3-GGCTGACCT (SEQ ID NO: 483) (SEQ ID NO: 484) 2.87 5'-PO3-GGTCTGCGT 5'-PO3-GCAGACCCT (SEQ ID NO: 485) (SEQ ID NO: 486) 2.88 5'-PO3-TAGCCGAGT 5'-PO3-TCGGCTACT (SEQ ID NO: 487) (SEQ ID NO: 488) 2.89 5'-PO3-GTCAGCAGT 5'-PO3-TGCTGACCT (SEQ ID NO: 489) (SEQ ID NO: 490) 2.90 5'-PO3-GGTCTGAGT 5'-PO3-TCAGACCCT (SEQ ID NO: 491) (SEQ ID NO: 492) 2.91 5'-PO3-CGGACAGGT 5'-PO3-CTGTCCGCT (SEQ ID NO: 493) (SEQ ID NO: 494) 2.92 5'-PO3-TTAGCCGGT5'- 5'-PO3-CGGCTAACT5'- PO3-3' PO3-3' (SEQ ID NO: 495) (SEQ ID NO: 496) 2.93 5'-PO3-GAGACGAGT 5'-PO3-TCGTCTCCT (SEQ ID NO: 497) (SEQ ID NO: 498) 2.94 5'-PO3-CGTAACCGT 5'-PO3-GGTTACGCT (SEQ ID NO: 499) (SEQ ID NO: 500) 2.95 5'-PO3-TTGGCGTGT5'- 5'-PO3-ACGCCAACT5'- PO3-3' PO3-3' (SEQ ID NO: 501) (SEQ ID NO: 502) 2.96 5'-PO3-ATGGCAGGT 5'-PO3-CTGCCATCT (SEQ ID NO: 503) (SEQ ID NO: 504)

TABLE-US-00007 TABLE 5 Oligonucleotide tags used in cycle 3 Tag Top Bottom number strand sequence strand sequence 3.1 5'-PO3-CAG CTA CGA 5'-PO3-GTA GCT GAC (SEQ ID NO: 505) (SEQ ID NO: 506) 3.2 5'-PO3-CTC CTG CGA 5'-PO3-GCA GGA GAC (SEQ ID NO: 507) (SEQ ID NO: 508) 3.3 5'-PO3-GCT GCC TGA 5'-PO3-AGG CAG CAC (SEQ ID NO: 509) (SEQ ID NO: 510) 3.4 5'-PO3-CAG GAA CGA 5'-PO3-GTT CCT GAC (SEQ ID NO: 511) (SEQ ID NO: 512) 3.5 5'-PO3-CAC ACG CGA 5'-PO3-GCG TGT GAC (SEQ ID NO: 513) (SEQ ID NO: 514) 3.6 5'-PO3-GCA GCC TGA 5'-PO3-AGG CTG CAC (SEQ ID NO: 515) (SEQ ID NO: 516) 3.7 5'-PO3-CTG AAC GGA 5'-PO3-CGT TCA GAC (SEQ ID NO: 517) (SEQ ID NO: 518) 3.8 5'-PO3-CTG AAC CGA 5'-PO3-GGT TCA GAC (SEQ ID NO: 519) (SEQ ID NO: 520) 3.9 5'-PO3-TCT GGA CGA 5'-PO3-GTC CAG AAC (SEQ ID NO: 521) (SEQ ID NO: 522) 3.10 5'-PO3-TGC CTA CGA 5'-PO3-GTA GGC AAC (SEQ ID NO: 523) (SEQ ID NO: 524) 3.11 5'-PO3-GGC ATA CGA 5'-PO3-GTA TGC CAC (SEQ ID NO: 525) (SEQ ID NO: 526) 3.12 5'-PO3-CGG TGA CGA 5'-PO3-GTC ACC GAC (SEQ ID NO: 527) (SEQ ID NO: 528) 3.13 5'-PO3-CAA CGA CGA 5'-PO3-GTC GTT GAC (SEQ ID NO: 529) (SEQ ID NO: 530) 3.14 5'-PO3-CTC CTC TGA 5'-PO3-AGA GGA GAC (SEQ ID NO: 531) (SEQ ID NO: 532) 3.15 5'-PO3-TCA GGA CGA 5'-PO3-GTC CTG AAC (SEQ ID NO: 533) (SEQ ID NO: 534) 3.16 5'-PO3-AAA GGC GGA 5'-PO3-CGC CTT TAC (SEQ ID NO: 535) (SEQ ID NO: 536) 3.17 5'-PO3-CTC CTC GGA 5'-PO3-CGA GGA GAC (SEQ ID NO: 537) (SEQ ID NO: 538) 3.18 5'-PO3-CAG ATG CGA 5'-PO3-GCA TCT GAC (SEQ ID NO: 539) (SEQ ID NO: 540) 3.19 5'-PO3-GCA GCA AGA 5'-PO3-TTG CTG CAC (SEQ ID NO: 541) (SEQ ID NO: 542) 3.20 5'-PO3-GTG GAG TGA 5'-PO3-ACT CCA CAC (SEQ ID NO: 543) (SEQ ID NO: 544) 3.21 5'-PO3-CCA GTA GGA 5'-PO3-CTA CTG GAC (SEQ ID NO: 545) (SEQ ID NO: 546) 3.22 5'-PO3-ATG GCA CGA 5'-PO3-GTG CCA TAC (SEQ ID NO: 547) (SEQ ID NO: 548) 3.23 5'-PO3-GGA CTG TGA 5'-PO3-ACA GTC CAC (SEQ ID NO: 549) (SEQ ID NO: 550) 3.24 5'-PO3-CCG AAC TGA 5'-PO3-AGT TCG GAC (SEQ ID NO: 551) (SEQ ID NO: 552) 3.25 5'-PO3-CTC CTC AGA 5'-PO3-TGA GGA GAC (SEQ ID NO: 553) (SEQ ID NO: 554) 3.26 5'-PO3-CAC TGC TGA 5'-PO3-AGC AGT GAC (SEQ ID NO: 555) (SEQ ID NO: 556) 3.27 5'-PO3-AGC AGG CGA 5'-PO3-GCC TGC TAC (SEQ ID NO: 557) (SEQ ID NO: 558) 3.28 5'-PO3-AGC AGG AGA 5'-PO3-TCC TGC TAC (SEQ ID NO: 559) (SEQ ID NO: 560) 3.29 5'-PO3-AGA GCC AGA 5'-PO3-TGG CTC TAC (SEQ ID NO: 561) (SEQ ID NO: 562) 3.30 5'-PO3-GTC GTT GGA 5'-PO3-CAA CGA CAC (SEQ ID NO: 563) (SEQ ID NO: 564) 3.31 5'-PO3-CCG AAC GGA 5'-PO3-CGT TCG GAC (SEQ ID NO: 565) (SEQ ID NO: 566) 3.32 5'-PO3-CAC TGC GGA 5'-PO3-CGC AGT GAC (SEQ ID NO: 567) (SEQ ID NO: 568) 3.33 5'-PO3-GTG GAG CGA 5'-PO3-GCT CCA CAC (SEQ ID NO: 569) (SEQ ID NO: 570) 3.34 5'-PO3-GTG GAG AGA 5'-PO3-TCT CCA CAC (SEQ ID NO: 571) (SEQ ID NO: 572) 3.35 5'-PO3-GGA CTG CGA 5'-PO3-GCA GTC CAC (SEQ ID NO: 573) (SEQ ID NO: 574) 3.36 5'-PO3-CCG AAC CGA 5'-PO3-GGT TCG GAC (SEQ ID NO: 575) (SEQ ID NO: 576) 3.37 5'-PO3-CAC TGC CGA 5'-PO3-GGC AGT GAC (SEQ ID NO: 577) (SEQ ID NO: 578) 3.38 5'-PO3-CGA AAC GGA 5'-PO3-CGT TTC GAC (SEQ ID NO: 579) (SEQ ID NO: 580) 3.39 5'-PO3-GGA CTG AGA 5'-PO3-TCA GTC CAC (SEQ ID NO: 581) (SEQ ID NO: 582) 3.40 5'-PO3-CCG AAC AGA 5'-PO3-TGT TCG GAC (SEQ ID NO: 583) (SEQ ID NO: 584) 3.41 5'-PO3-CGA AAC CGA 5'-PO3-GGT TTC GAC (SEQ ID NO: 585) (SEQ ID NO: 586) 3.42 5'-PO3-CTG GCT TGA 5'-PO3-AAG CCA GAC (SEQ ID NO: 587) (SEQ ID NO: 588) 3.43 5'-PO3-CAC ACC TGA 5'-PO3-AGG TGT GAC (SEQ ID NO: 589) (SEQ ID NO: 590) 3.44 5'-PO3-AAC GAC CGA 5'-PO3-GGT CGT TAC (SEQ ID NO: 591) (SEQ ID NO: 592) 3.45 5'-PO3-ATC CAG CGA 5'-PO3-GCT GGA TAC (SEQ ID NO: 593) (SEQ ID NO: 594) 3.46 5'-PO3-TGC GAA GGA 5'-PO3-CTT CGC AAC (SEQ ID NO: 595) (SEQ ID NO: 596) 3.47 5'-PO3-TGC GAA CGA 5'-PO3-GTT CGC AAC (SEQ ID NO: 597) (SEQ ID NO: 598) 3.48 5'-PO3-CTG GCT GGA 5'-PO3-CAG CCA GAC (SEQ ID NO: 599) (SEQ ID NO: 600) 3.49 5'-PO3-CAC ACC GGA 5'-PO3-CGG TGT GAC (SEQ ID NO: 601) (SEQ ID NO: 602) 3.50 5'-PO3-AGT GCA GGA 5'-PO3-CTG CAC TAC (SEQ ID NO: 603) (SEQ ID NO: 604) 3.51 5'-PO3-GAC CGT TGA 5'-PO3-AAC GGT CAC (SEQ ID NO: 605) (SEQ ID NO: 606) 3.52 5'-PO3-GGT GAG TGA 5'-PO3-ACT CAC CAC (SEQ ID NO: 607) (SEQ ID NO: 608) 3.53 5'-PO3-CCT TCC TGA 5'-PO3-AGG AAG GAC (SEQ ID NO: 609) (SEQ ID NO: 610) 3.54 5'-PO3-CTG GCT AGA 5'-PO3-TAG CCA GAC (SEQ ID NO: 611) (SEQ ID NO: 612) 3.55 5'-PO3-CAC ACC AGA 5'-PO3-TGG TGT GAC (SEQ ID NO: 613) (SEQ ID NO: 614) 3.56 5'-PO3-AGC GGT AGA 5'-PO3-TAC CGC TAC (SEQ ID NO: 615) (SEQ ID NO: 616) 3.57 5'-PO3-GTC AGA GGA 5'-PO3-CTC TGA CAC (SEQ ID NO: 617) (SEQ ID NO: 618) 3.58 5'-PO3-TTC CGA CGA 5'-PO3-GTC GGA AAC (SEQ ID NO: 619) (SEQ ID NO: 620) 3.59 5'-PO3-AGG CGT AGA 5'-PO3-TAC GCC TAC (SEQ ID NO: 621) (SEQ ID NO: 622) 3.60 5'-PO3-CTC GAC TGA 5'-PO3-AGT CGA GAC (SEQ ID NO: 623) (SEQ ID NO: 624) 3.61 5'-PO3-TAC GCT GGA 5'-PO3-CAG CGT AAC (SEQ ID NO: 625) (SEQ ID NO: 626) 3.62 5'-PO3-GTT CGG TGA 5'-PO3-ACC GAA CAC (SEQ ID NO: 627) (SEQ ID NO: 628) 3.63 5'-PO3-GCC AGC AGA 5'-PO3-TGC TGG CAC (SEQ ID NO: 629) (SEQ ID NO: 630) 3.64 5'-PO3-GAC CGT AGA 5'-PO3-TAC GGT CAC (SEQ ID NO: 631) (SEQ ID NO: 632) 3.65 5'-PO3-GTG CTC TGA 5'-PO3-AGA GCA CAC (SEQ ID NO: 633) (SEQ ID NO: 634) 3.66 5'-PO3-GGT GAG CGA 5'-PO3-GCT CAC CAC (SEQ ID NO: 635) (SEQ ID NO: 636) 3.67 5'-PO3-GGT GAG AGA 5'-PO3-TCT CAC CAC (SEQ ID NO: 637) (SEQ ID NO: 638) 3.68 5'-PO3-CCT TCC AGA 5'-PO3-TGG AAG GAC (SEQ ID NO: 639) (SEQ ID NO: 640) 3.69 5'-PO3-CTC CTA CGA 5'-PO3-GTA GGA GAC (SEQ ID NO: 641) (SEQ ID NO: 642) 3.70 5'-PO3-CTC GAC GGA 5'-PO3-CGT CGA GAC (SEQ ID NO: 643) (SEQ ID NO: 644) 3.71 5'-PO3-GCC GTT TGA 5'-PO3-AAA CGG CAC (SEQ ID NO: 645) (SEQ ID NO: 646) 3.72 5'-PO3-GCG GAG TGA 5'-PO3-ACT CCG CAC (SEQ ID NO: 647) (SEQ ID NO: 648) 3.73 5'-PO3-CGT GCT TGA 5'-PO3-AAG CAC GAC (SEQ ID NO: 649) (SEQ ID NO: 650) 3.74 5'-PO3-CTC GAC CGA 5'-PO3-GGT CGA GAC (SEQ ID NO: 651) (SEQ ID NO: 652) 3.75 5'-PO3-AGA GCA GGA 5'-PO3-CTG CTC TAC (SEQ ID NO: 653) (SEQ ID NO: 654) 3.76 5'-PO3-GTG CTC GGA 5'-PO3-CGA GCA CAC (SEQ ID NO: 655) (SEQ ID NO: 656) 3.77 5'-PO3-CTC GAC AGA 5'-PO3-TGT CGA GAC (SEQ ID NO: 657) (SEQ ID NO: 658) 3.78 5'-PO3-GGA GAG TGA 5'-PO3-ACT CTC CAC (SEQ ID NO: 659) (SEQ ID NO: 660) 3.79 5'-PO3-AGG CTG TGA 5'-PO3-ACA GCC TAC (SEQ ID NO: 661) (SEQ ID NO: 662) 3.80 5'-PO3-AGA GCA CGA 5'-PO3-GTG CTC TAC (SEQ ID NO: 663) (SEQ ID NO: 664) 3.81 5'-PO3-CCA TCC TGA 5'-PO3-AGG ATG GAC (SEQ ID NO: 665) (SEQ ID NO: 666) 3.82 5'-PO3-GTT CGG AGA 5'-PO3-TCC GAA CAC

(SEQ ID NO: 667) (SEQ ID NO: 668) 3.83 5'-PO3-TGG TAG CGA 5'-PO3-GCT ACC AAC (SEQ ID NO: 669) (SEQ ID NO: 670) 3.84 5'-PO3-GTG CTC CGA 5'-PO3-GGA GCA CAC (SEQ ID NO: 671) (SEQ ID NO: 672) 3.85 5'-PO3-GTG CTC AGA 5'-PO3-TGA GCA CAC (SEQ ID NO: 673) (SEQ ID NO: 674) 3.86 5'-PO3-GCC GTT GGA 5'-PO3-CAA CGG CAC (SEQ ID NO: 675) (SEQ ID NO: 676) 3.87 5'-PO3-GAG TGC TGA 5'-PO3-AGC ACT CAC (SEQ ID NO: 677) (SEQ ID NO: 678) 3.88 5'-PO3-GCT CCT TGA 5'-PO3-AAG GAG CAC (SEQ ID NO: 679) (SEQ ID NO: 680) 3.89 5'-PO3-CCG AAA GGA 5'-PO3-CTT TCG GAC (SEQ ID NO: 681) (SEQ ID NO: 682) 3.90 5'-PO3-CAC TGA GGA 5'-PO3-CTC AGT GAC (SEQ ID NO: 683) (SEQ ID NO: 684) 3.91 5'-PO3-CGT GCT GGA 5'-PO3-CAG CAC GAC (SEQ ID NO: 685) (SEQ ID NO: 686) 3.92 5'-PO3-CCG AAA CGA 5'-PO3-GTT TCG GAC (SEQ ID NO: 687) (SEQ ID NO: 688) 3.93 5'-PO3-GCG GAG AGA 5'-PO3-TCT CCG CAC (SEQ ID NO: 689) (SEQ ID NO: 690) 3.94 5'-PO3-GCC GTT AGA 5'-PO3-TAA CGG CAC (SEQ ID NO: 691) (SEQ ID NO: 692) 3.95 5'-PO3-TCT CGT GGA 5'-PO3-CAC GAG AAC (SEQ ID NO: 693) (SEQ ID NO: 694) 3.96 5'-PO3-CGT GCT AGA 5'-PO3-TAG CAC GAC (SEQ ID NO: 695) (SEQ ID NO: 696)

TABLE-US-00008 TABLE 6 Oligonucleotide tags used in cycle 4 Tag Top Bottom number strand sequence strand sequence 4.1 5'-PO3-GCCTGTCTT 5'-PO3-GAC AGG CTC (SEQ ID NO: 697) (SEQ ID NO: 698) 4.2 5'-PO3-CTCCTGGTT 5'-PO3-CCA GGA GTC (SEQ ID NO: 699) (SEQ ID NO: 700) 4.3 5'-PO3-ACTCTGCTT 5'-PO3-GCA GAG TTC (SEQ ID NO: 701) (SEQ ID NO: 702) 4.4 5'-PO3-CATCGCCTT 5'-PO3-GGC GAT GTC (SEQ ID NO: 703) (SEQ ID NO: 704) 4.5 5'-PO3-GCCACTATT 5'-PO3-TAG TGG CTC (SEQ ID NO: 705) (SEQ ID NO: 706) 4.6 5'-PO3-CACACGGTT 5'-PO3-CCG TGT GTC (SEQ ID NO: 707) (SEQ ID NO: 708) 4.7 5'-PO3-CAACGCCTT 5'-PO3-GGC GTT GTC (SEQ ID NO: 709) (SEQ ID NO: 710) 4.8 5'-PO3-ACTGAGGTT 5'-PO3-CCT CAG TTC (SEQ ID NO: 711) (SEQ ID NO: 712) 4.9 5'-PO3-GTGCTGGTT 5'-PO3-CCA GCA CTC (SEQ ID NO: 713) (SEQ ID NO: 714) 4.10 5'-PO3-CATCGACTT 5'-PO3-GTC GAT GTC (SEQ ID NO: 715) (SEQ ID NO: 716) 4.11 5'-PO3-CCATCGGTT 5'-PO3-CCG ATG GTC (SEQ ID NO: 717) (SEQ ID NO: 718) 4.12 5'-PO3-GCTGCACTT 5'-PO3-GTG CAG CTC (SEQ ID NO: 719) (SEQ ID NO: 720) 4.13 5'-PO3-ACAGAGGTT 5'-PO3-CCT CTG TTC (SEQ ID NO: 721) (SEQ ID NO: 722) 4.14 5'-PO3-AGTGCCGTT 5'-PO3-ATG TCC GTC (SEQ ID NO: 723) (SEQ ID NO: 724) 4.15 5'-PO3-CGGACATTT 5'-PO3-ATG TCC GTC (SEQ ID NO: 725) (SEQ ID NO: 726) 4.16 5'-PO3-GGTCTGGTT 5'-PO3-CCA GAC CTC (SEQ ID NO: 727) (SEQ ID NO: 728) 4.17 5'-PO3-GAGACGGTT 5'-PO3-CCG TCT CTC (SEQ ID NO: 729) (SEQ ID NO: 730) 4.18 5'-PO3-CTTTCCGTT 5'-PO3-CGG AAA GTC (SEQ ID NO: 731) (SEQ ID NO: 732) 4.19 5'-PO3-CAGATGGTT 5'-PO3-CCA TCT GTC (SEQ ID NO: 733) (SEQ ID NO: 734) 4.20 5'-PO3-CGGACACTT 5'-PO3-GTG TCC GTC (SEQ ID NO: 735) (SEQ ID NO: 736) 4.21 5'-PO3-ACTCTCGTT 5'-PO3-CGA GAG TTC (SEQ ID NO: 737) (SEQ ID NO: 738) 4.22 5'-PO3-GCAGCACTT 5'-PO3-GTG CTG CTC (SEQ ID NO: 739) (SEQ ID NO: 740) 4.23 5'-PO3-ACTCTCCTT 5'-PO3-GGA GAG TTC (SEQ ID NO: 741) (SEQ ID NO: 742) 4.24 5'-PO3-ACCTTGGTT 5'-PO3-CCA AGG TTC (SEQ ID NO: 743) (SEQ ID NO: 744) 4.25 5'-PO3-AGAGCCGTT 5'-PO3-CGG CTC TTC (SEQ ID NO: 745 (SEQ ID NO: 746) 4.26 5'-PO3-ACCTTGCTT 5'-PO3-GCA AGG TTC (SEQ ID NO: 747) (SEQ ID NO: 748) 4.27 5'-PO3-AAGTCCGTT 5'-PO3-CGG ACT TTC (SEQ ID NO: 749) (SEQ ID NO: 750) 4.28 5'-PO3-GGA CTG GTT 5'-PO3-CCA GTC CTC (SEQ ID NO: 751) (SEQ ID NO: 752) 4.29 5'-PO3-GTCGTTCTT 5'-PO3-GAA CGA CTC (SEQ ID NO: 753) (SEQ ID NO: 754) 4.30 5'-PO3-CAGCATCTT 5'-PO3-GAT GCT GTC (SEQ ID NO: 755) (SEQ ID NO: 756) 4.31 5'-PO3-CTATCCGTT 5'-PO3-CGG ATA GTC (SEQ ID NO: 757) (SEQ ID NO: 758) 4.32 5'-PO3-ACACTCGTT 5'-PO3-CGA GTG TTC (SEQ ID NO: 759) (SEQ ID NO: 760) 4.33 5'-PO3-ATCCAGGTT 5'-PO3-CCT GGA TTC (SEQ ID NO: 761) (SEQ ID NO: 762) 4.34 5'-PO3-GTTCCTGTT 5'-PO3-CAG GAA CTC (SEQ ID NO: 763) (SEQ ID NO: 764) 4.35 5'-PO3-ACACTCCTT 5'-PO3-GGA GTG TTC (SEQ ID NO: 765) (SEQ ID NO: 766) 4.36 5'-PO3-GTTCCTCTT 5'-PO3-GAG GAA CTC (SEQ ID NO: 767) (SEQ ID NO: 768) 4.37 5'-PO3-CTGGCTCTT 5'-PO3-GAG CCA GTC (SEQ ID NO: 769) (SEQ ID NO: 770) 4.38 5'-PO3-ACGGCATTT 5'-PO3-ATG CCG TTC (SEQ ID NO: 771) (SEQ ID NO: 772) 4.39 5'-PO3-GGTGAGGTT 5'-PO3-CCT CAC CTC (SEQ ID NO: 773) (SEQ ID NO: 774) 4.40 5'-PO3-CCTTCCGTT 5'-PO3-CGG AAG GTC (SEQ ID NO: 775) (SEQ ID NO: 776) 4.41 5'-PO3-TACGCTCTT 5'-PO3-GAG CGT ATC (SEQ ID NO: 777) (SEQ ID NO: 778) 4.42 5'-PO3-ACGGCAGTT 5'-PO3-CTG CCG TTC (SEQ ID NO: 779) (SEQ ID NO: 780) 4.43 5'-PO3-ACTGACGTT 5'-PO3-CGT CAG TTC (SEQ ID NO: 781) (SEQ ID NO: 782) 4.44 5'-PO3-ACGGCACTT 5'-PO3-GTG CCG TTC (SEQ ID NO: 783) (SEQ ID NO: 784) 4.45 5'-PO3-ACTGACCTT 5'-PO3-GGT CAG TTC (SEQ ID NO: 785) (SEQ ID NO: 786) 4.46 5'-PO3-TTTGCGGTT 5'-PO3-CCG CAA ATC (SEQ ID NO: 787) (SEQ ID NO: 788) 4.47 5'-PO3-TGGTAGGTT 5'-PO3-CCT ACC ATC (SEQ ID NO: 789) (SEQ ID NO: 790) 4.48 5'-PO3-GTTCGGCTT 5'-PO3-GCC GAA CTC (SEQ ID NO: 791) (SEQ ID NO: 792) 4.49 5'-PO3-GCC GTT CTT 5'-PO3-GAA CGG CTC (SEQ ID NO: 793) (SEQ ID NO: 794) 4.50 5'-PO3-GGAGAGGTT 5'-PO3-CCT CTC CTC (SEQ ID NO: 795) (SEQ ID NO: 796) 4.51 5'-PO3-CACTGACTT 5'-PO3-GTC AGT GTC (SEQ ID NO: 797) (SEQ ID NO: 798) 4.52 5'-PO3-CGTGCTCTT 5'-PO3-GAG CAC GTC (SEQ ID NO:799) (SEQ ID NO: 800) 4.53 5'-PO3-AATCCGCTT 5'-PO3-GCGGATTTC (SEQ ID NO: 801) (SEQ ID NO: 802) 4.54 5'-PO3-AGGCTGGTT 5'-PO3-CCA GCC TTC (SEQ ID NO: 803) (SEQ ID NO: 804) 4.55 5'-PO3-GCTAGTGTT 5'-PO3-CAC TAG CTC (SEQ ID NO: 805) (SEQ ID NO: 806) 4.56 5'-PO3-GGAGAGCTT 5'-PO3-GCT CTC CTC (SEQ ID NO: 807) (SEQ ID NO: 808) 4.57 5'-PO3-GGAGAGATT 5'-PO3-TCT CTC CTC (SEQ ID NO: 809) (SEQ ID NO: 810) 4.58 5'-PO3-AGGCTGCTT 5'-PO3-GCA GCC TTC (SEQ ID NO: 811) (SEQ ID NO: 812) 4.59 5'-PO3-GAGTGCGTT 5'-PO3-CGC ACT CTC (SEQ ID NO: 813) (SEQ ID NO: 814) 4.60 5'-PO3-CCATCCATT 5'-PO3-TGG ATG GTC (SEQ ID NO: 815) (SEQ ID NO: 816) 4.61 5'-PO3-GCTAGTCTT 5'-PO3-GAC TAG CTC (SEQ ID NO: 817) (SEQ ID NO: 818) 4.62 5'-PO3-AGGCTGATT 5'-PO3-TCA GCC TTC (SEQ ID NO: 819) (SEQ ID NO: 820) 4.63 5'-PO3-ACAGACGTT 5'-PO3-CGT CTG TTC (SEQ ID NO: 821) (SEQ ID NO: 822) 4.64 5'-PO3-GAGTGCCTT 5'-PO3-GGC ACT CTC (SEQ ID NO: 823) (SEQ ID NO: 824) 4.65 5'-PO3-ACAGACCTT 5'-PO3-GGT CTG TTC (SEQ ID NO: 825) (SEQ ID NO: 826) 4.66 5'-PO3-CGAGCTTTT 5'-PO3-AAG CTC GTC (SEQ ID NO: 827) (SEQ ID NO: 828) 4.67 5'-PO3-TTAGCGGTT 5'-PO3-CCG CTA ATC (SEQ ID NO: 829) (SEQ ID NO: 830) 4.68 5'-PO3-CCTCTTGTT 5'-PO3-CAA GAG GTC (SEQ ID NO: 831) (SEQ ID NO: 832) 4.69 5'-PO3-GGTCTCTTT 5'-PO3-AGA GAC CTC (SEQ ID NO: 833) (SEQ ID NO: 834) 4.70 5'-PO3-GCCAGATTT 5'-PO3-ATC TGG CTC (SEQ ID NO: 835) (SEQ ID NO: 836) 4.71 5'-PO3-GAGACCTTT 5'-PO3-AGG TCT CTC (SEQ ID NO: 837) (SEQ ID NO: 838) 4.72 5'-PO3-CACACAGTT 5'-PO3-CTG TGT GTC (SEQ ID NO: 839) (SEQ ID NO: 840) 4.73 5'-PO3-CCTCTTCTT 5'-PO3-GAA GAG GTC (SEQ ID NO: 841) (SEQ ID NO: 842) 4.74 5'-PO3-TAGAGCGTT 5'-PO3-CGC TCT ATC (SEQ ID NO: 843) (SEQ ID NO: 844) 4.75 5'-PO3-GCACCTTTT 5'-PO3-AAG GTG CTC (SEQ ID NO: 845) (SEQ ID NO: 846) 4.76 5'-PO3-GGCTTGTTT 5'-PO3-ACA AGC CTC (SEQ ID NO: 847) (SEQ ID NO: 848) 4.77 5'-PO3-GACGCGATT 5'-PO3-TCG CGT CTC (SEQ ID NO: 849) (SEQ ID NO: 850) 4.78 5'-PO3-CGAGCTGTT 5'-PO3-CAG CTC GTC (SEQ ID NO: 851) (SEQ ID NO: 852) 4.79 5'-PO3-TAGAGCCTT 5'-PO3-GGC TCT ATC (SEQ ID NO: 853) (SEQ ID NO: 854) 4.80 5'-PO3-CATCCGTTT 5'-PO3-ACG GAT GTC (SEQ ID NO: 855) (SEQ ID NO: 856) 4.81 5'-PO3-GGTCTCGTT 5'-PO3-CGA GAC CTC (SEQ ID NO: 857) (SEQ ID NO: 858) 4.82 5'-PO3-GCCAGAGTT 5'-PO3-CTC TGG CTC

(SEQ ID NO: 859) (SEQ ID NO: 860) 4.83 5'-PO3-GAGACCGTT 5'-PO3-CGG TCT CTC (SEQ ID NO: 861) (SEQ ID NO: 862) 4.84 5'-PO3-CGAGCTATT 5'-PO3-TAG CTC GTC (SEQ ID NO: 863) (SEQ ID NO: 864) 4.85 5'-PO3-GCAAGTGTT 5'-PO3-CAC TTG CTC (SEQ ID NO: 865) (SEQ ID NO: 866) 4.86 5'-PO3-GGTCTCCTT 5'-PO3-GGA GAC CTC (SEQ ID NO: 867) (SEQ ID NO: 868) 4.87 5'-PO3-GCCAGACTT 5'-PO3-GTC TGG CTC (SEQ ID NO: 869) (SEQ ID NO: 870) 4.88 5'-PO3-GGTCTCATT 5'-PO3-TGA GAC CTC (SEQ ID NO: 871) (SEQ ID NO: 872) 4.89 5'-PO3-GAGACCATT 5'-PO3-TGG TCT CTC (SEQ ID NO: 873) (SEQ ID NO: 874) 4.90 5'-PO3-CCTTCAGTT 5'-PO3-CTG AAG GTC (SEQ ID NO: 875) (SEQ ID NO: 876) 4.91 5'-PO3-GCACCTGTT 5'-PO3-CAG GTG CTC (SEQ ID NO: 877) (SEQ ID NO: 878) 4.92 5'-PO3-AAAGGCGTT 5'-PO3-CGC CTT TTC (SEQ ID NO: 879) (SEQ ID NO: 880) 4.93 5'-PO3-CAGATCGTT 5'-PO3-CGA TCT GTC (SEQ ID NO: 881) (SEQ ID NO: 882) 4.94 5'-PO3-CATAGGCTT 5'-PO3-GCC TAT GTC (SEQ ID NO: 883) (SEQ ID NO: 884) 4.95 5'-PO3-CCTTCACTT 5'-PO3-GTG AAG GTC (SEQ ID NO: 885) (SEQ ID NO: 886) 4.96 5'-PO3-GCACCTCTT 5'-PO3-GAG GTG CTC (SEQ ID NO: 887) (SEQ ID NO: 888)

TABLE-US-00009 TABLE 7 Correspondence between building blocks and oligonucleotide tags for Cycles 1-4. Building block Cycle 1 Cycle 2 Cycle 3 Cycle 4 BB1 1.1 2.1 3.1 4.1 BB2 1.2 2.2 3.2 4.2 BB3 1.3 2.3 3.3 4.3 BB4 1.4 2.4 3.4 4.4 BB5 1.5 2.5 3.5 4.5 BB6 1.6 2.6 3.6 4.6 BB7 1.7 2.7 3.7 4.7 BB8 1.8 2.8 3.8 4.8 BB9 1.9 2.9 3.9 4.9 BB10 1.10 2.10 3.10 4.10 BB11 1.11 2.11 3.11 4.11 BB12 1.12 2.12 3.12 4.12 BB13 1.13 2.13 3.13 4.13 BB14 1.14 2.14 3.14 4.14 BB15 1.15 2.15 3.15 4.15 BB16 1.16 2.16 3.16 4.16 BB17 1.17 2.17 3.17 4.17 BB18 1.18 2.18 3.18 4.18 BB19 1.19 2.19 3.19 4.19 BB20 1.20 2.20 3.20 4.20 BB21 1.21 2.21 3.21 4.21 BB22 1.22 2.22 3.22 4.22 BB23 1.23 2.23 3.23 4.23 BB24 1.24 2.24 3.24 4.24 BB25 1.25 2.25 3.25 4.25 BB26 1.26 2.26 3.26 4.26 BB27 1.27 2.27 3.27 4.27 BB28 1.28 2.28 3.28 4.28 BB29 1.29 2.29 3.29 4.29 BB30 1.30 2.30 3.30 4.30 BB31 1.31 2.31 3.31 4.31 BB32 1.32 2.32 3.32 4.32 BB33 1.33 2.33 3.33 4.33 BB34 1.34 2.34 3.34 4.34 BB35 1.35 2.35 3.35 4.35 BB36 1.36 2.36 3.36 4.36 BB37 1.37 2.37 3.37 4.37 BB38 1.38 2.38 3.38 4.38 BB39 1.39 2.39 3.39 4.39 BB40 1.44 2.44 3.44 4.44 BB41 1.41 2.41 3.41 4.41 BB42 1.42 2.42 3.42 4.42 BB43 1.43 2.43 3.43 4.43 BB44 1.40 2.40 3.40 4.40 BB45 1.45 2.45 3.45 4.45 BB46 1.46 2.46 3.46 4.46 BB47 1.47 2.47 3.47 4.47 BB48 1.48 2.48 3.48 4.48 BB49 1.49 2.49 3.49 4.49 BB50 1.50 2.50 3.50 4.50 BB51 1.51 2.51 3.51 4.51 BB52 1.52 2.52 3.52 4.52 BB53 1.53 2.53 3.53 4.53 BB54 1.54 2.54 3.54 4.54 BB55 1.55 2.55 3.55 4.55 BB56 1.56 2.56 3.56 4.56 BB57 1.57 2.57 3.57 4.57 BB58 1.58 2.58 3.58 4.58 BB59 1.59 2.59 3.59 4.59 BB60 1.60 2.60 3.60 4.60 BB61 1.61 2.61 3.61 4.61 BB62 1.62 2.62 3.62 4.62 BB63 1.63 2.63 3.63 4.63 BB64 1.64 2.64 3.64 4.64 BB65 1.65 2.65 3.65 4.65 BB66 1.66 2.66 3.66 4.66 BB67 1.67 2.67 3.67 4.67 BB68 1.68 2.68 3.68 4.68 BB69 1.69 2.69 3.69 4.69 BB70 1.70 2.70 3.70 4.70 BB71 1.71 2.71 3.71 4.71 BB72 1.72 2.72 3.72 4.72 BB73 1.73 2.73 3.73 4.73 BB74 1.74 2.74 3.74 4.74 BB75 1.75 2.75 3.75 4.75 BB76 1.76 2.76 3.76 4.76 BB77 1.77 2.77 3.77 4.77 BB78 1.78 2.78 3.78 4.78 BB79 1.79 2.79 3.79 4.79 BB80 1.80 2.80 3.80 4.80 BB81 1.81 2.81 3.81 4.81 BB82 1.82 2.82 3.82 4.82 BB83 1.96 2.96 3.96 4.96 BB84 1.83 2.83 3.83 4.83 BB85 1.84 2.84 3.84 4.84 BB86 1.85 2.85 3.85 4.85 BB87 1.86 2.86 3.86 4.86 BB88 1.87 2.87 3.87 4.87 BB89 1.88 2.88 3.88 4.88 BB90 1.89 2.89 3.89 4.89 BB91 1.90 2.90 3.90 4.90 BB92 1.91 2.91 3.91 4.91 BB93 1.92 2.92 3.92 4.92 BB94 1.93 2.93 3.93 4.93 BB95 1.94 2.94 3.94 4.94 BB96 1.95 2.95 3.95 4.95 1X ligase buffer: 50 mM Tris, pH 7.5; 10 mM dithiothreitol; 10 mM MgCl.sub.2; 2 mM ATP; 50 mM NaCl. 10X ligase buffer: 500 mM Tris, pH 7.5; 100 mM dithiothreitol; 100 mM MgCl.sub.2; 20 mM ATP; 500 mM NaCl

Attachment of Water Soluble Spacer to Compound 2

[0139] To a solution of Compound 2 (60 mL, 1 mM) in sodium borate buffer (150 mM, pH 9.4) that was chilled to 4.degree. C. was added 40 equivalents of N-Fmoc-15-amino-4,7,10,13-tetraoxaoctadecanoic acid (S-Ado) in N,N-dimethylformamide (DMF) (16 mL, 0.15 M) followed by 40 equivalents of 4-(4,6-dimethoxy[1.3.5]triazin-2-yl)-4-methylmorpholinium chloride hydrate (DMTMM) in water (9.6 mL, 0.25 M). The mixture was gently shaken for 2 hours at 4.degree. C. before an additional 40 equivalents of S-Ado and DMTMM were added and shaken for a further 16 hours at 4.degree. C.

[0140] Following acylation, a 0.1.times. volume of 5 M aqueous NaCl and a 2.5.times. volume of cold (-20.degree. C.) ethanol was added and the mixture was allowed to stand at -20.degree. C. for at least one hour. The mixture was then centrifuged for 15 minutes at 14,000 rpm in a 4.degree. C. centrifuge to give a white pellet which was washed with cold EtOH and then dried in a lyophilizer at room temperature for 30 minutes. The solid was dissolved in 40 mL of water and purified by Reverse Phase HPLC with a Waters Xterra RP.sub.18 column A binary mobile phase gradient profile was used to elute the product using a 50 mM aqueous triethylammonium acetate buffer at pH 7.5 and 99% acetonitrile/1% water solution. The purified material was concentrated by lyophilization and the resulting residue was dissolved in 5 mL of water. A 0.1.times. volume of piperidine was added to the solution and the mixture was gently shaken for 45 minutes at room temperature. The product was then purified by ethanol precipitation as described above and isolated by centrifugation. The resulting pellet was washed twice with cold EtOH and dried by lyophilization to give purified Compound 3.

Cycle 1

[0141] To each well in a 96 well plate was added 12.5 .mu.L of a 4 mM solution of Compound 3 in water; 100 .mu.L of a 1 mM solution of one of oligonucleotide tags 1.1 to 1.96, as shown in Table 3 (the molar ratio of Compound 3 to tags was 1:2). The plates were heated to 95.degree. C. for 1 minute and then cooled to 16.degree. C. over 10 minutes. To each well was added 10 .mu.L of 10.times. ligase buffer, 30 units T4 DNA ligase (1 .mu.L of a 30 unit/.mu.L solution (FermentasLife Science, Cat. No. EL0013)), 76.5 .mu.l of water and the resulting solutions were incubated at 16.degree. C. for 16 hours.

[0142] After the ligation reaction, 20 .mu.L of 5 M aqueous NaCl was added directly to each well, followed by 500 .mu.L cold (-20.degree. C.) ethanol, and held at -20.degree. C. for 1 hour. The plates were centrifugated for 1 hour at 3200 g in a Beckman Coulter Allegra 6R centrifuge using Beckman Microplus Carriers. The supernatant was carefully removed by inverting the plate and the pellet was washed with 70% aqueous cold ethanol at -20.degree. C. Each of the pellets was then dissolved in sodium borate buffer (50 .mu.L, 150 mM, pH 9.4) to a concentration of 1 mM and chilled to 4.degree. C.

[0143] To each solution was added 40 equivalents of one of the 96 building block precursors in DMF (13 .mu.L, 0.15 M) followed by 40 equivalents of DMT-MM in water (8 .mu.L, 0.25M), and the solutions were gently shaken at 4.degree. C. After 2 hours, an additional 40 equivalents of one of each building block precursor and DMTMM were added and the solutions were gently shaken for 16 hours at 4.degree. C. Following acylation, 10 equivalents of acetic acid-N-hydroxy-succinimide ester in DMF (2 .mu.L, 0.25M) was added to each solution and gently shaken for 10 minutes.

[0144] Following acylation, the 96 reaction mixtures were pooled and 0.1 volume of 5M aqueous NaCl and 2.5 volumes of cold absolute ethanol were added and the solution was allowed to stand at -20.degree. C. for at least one hour. The mixture was then centrifuged. Following centrifugation, as much supernatant as possible was removed with a micropipette, the pellet was washed with cold ethanol and centrifuged again. The supernatant was removed with a 200 .mu.L pipette. Cold 70% ethanol was added to the tube, and the resulting mixture was centrifuged for 5 min at 4.degree. C.

[0145] The supernatant was removed and the remaining ethanol was removed by lyophilization at room temperature for 10 minutes. The pellet was then dissolved in 2 mL of water and purified by Reverse Phase HPLC with a Waters Xterra RP.sub.18 column. A binary mobile phase gradient profile was used to elute the library using a 50 mM aqueous triethylammonium acetate buffer at pH 7.5 and 99% acetonitrile/1% water solution. The fractions containing the library were collected, pooled, and lyophilized. The resulting residue was dissolved in 2.5 mL of water and 250 .mu.L of piperidine was added. The solution was shaken gently for 45 minutes and then precipitated with ethanol as previously described. The resulting pellet was dried by lyophilization and then dissolved in sodium borate buffer (4.8 mL, 150 mM, pH 9.4) to a concentration of 1 mM.

[0146] The solution was chilled to 4.degree. C. and 40 equivalents each of N-Fmoc-propargylglycine in DMF (1.2 mL, 0.15 M) and DMT-MM in water (7.7 mL, 0.25 M) were added. The mixture was gently shaken for 2 hours at 4.degree. C. before an additional 40 equivalents of N-Fmoc-propargylglycine and DMT-MM were added and the solution was shaken for a further 16 hours. The mixture was later purified by EtOH precipitation and Reverse Phase HPLC as described above and the N-Fmoc group was removed by treatment with piperidine as previously described. Upon final purification by EtOH precipitation, the resulting pellet was dried by lyophilization and carried into the next cycle of synthesis

Cycles 2-4

[0147] For each of these cycles, the dried pellet from the previous cycle was dissolved in water and the concentration of library was determined by spectrophotometry based on the extinction coefficient of the DNA component of the library, where the initial extinction coefficient of Compound 2 is 131,500 L/(molecm). The concentration of the library was adjusted with water such that the final concentration in the subsequent ligation reactions was 0.25 mM. The library was then divided into 96 equal aliquots in a 96 well plate. To each well was added a solution comprising a different tag (molar ratio of the library to tag was 1:2), and ligations were performed as described for Cycle 1. Oligonucleotide tags used in Cycles 2, 3 and 4 are set forth in Tables 4, 5 and 6, respectively. Correspondence between the tags and the building block precursors for each of Cycles 1 to 4 is provided in Table 7. The library was precipitated by the addition of ethanol as described above for Cycle 1, and dissolved in sodium borate buffer (150 mM, pH 9.4) to a concentration of 1 mM. Subsequent acylations and purifications were performed as described for Cycle 1, except HPLC purification was omitted during Cycle 3.

[0148] The products of Cycle 4 were ligated with the closing primer shown below, using the method described above for ligation of tags.

TABLE-US-00010 (SEQ ID NO: 889) 5'-PO.sub.3-CAG AAG ACA GAC AAG CTT CAC CTG C (SEQ ID NO: 890) 5'-PO.sub.3-GCA GGT GAA GCT TGT CTG TCT TCT GAA

Results:

[0149] The synthetic procedure described above has the capability of producing a library comprising 96.sup.4 (about 10.sup.8) different structures. The synthesis of the library was monitored via gel electrophoresis and LC/MS of the product of each cycle. Upon completion, the library was analyzed using several techniques. FIG. 13a is a chromatogram of the library following Cycle 4, but before ligation of the closing primer; FIG. 13b is a mass spectrum of the library at the same synthetic stage. The average molecular weight was determined by negative ion LC/MS analysis. The ion signal was deconvoluted using ProMass software. This result is consistent with the predicted average mass of the library.

[0150] The DNA component of the library was analyzed by agarose gel electrophoresis, which showed that the majority of library material corresponds to ligated product of the correct size. DNA sequence analysis of molecular clones of PCR product derived from a sampling of the library shows that DNA ligation occurred with high fidelity and to near completion.

Library Cyclization

[0151] At the completion of Cycle 4, a portion of the library was capped at the N-terminus using azidoacetic acid under the usual acylation conditions. The product, after purification by EtOH precipitation, was dissolved in sodium phosphate buffer (150 mM, pH 8) to a concentration of 1 mM and 4 equivalents each of CuSO.sub.4 in water (200 mM), ascorbic acid in water (200 mM), and a solution of the compound shown below in DMF (200 mM) were added. The reaction mixture was then gently shaken for 2 hours at room temperature.

##STR00028##

[0152] To assay the extent of cyclization, 5 .mu.L aliquots from the library cyclization reaction were removed and treated with a fluorescently-labeled azide or alkyne (1 .mu.L of 100 mM DMF stocks) prepared as described in Example 4. After 16 hours, neither the alkyne or azide labels had been incorporated into the library by HPLC analysis at 500 nm. This result indicated that the library no longer contained azide or alkyne groups capable of cycloaddition and that the library must therefore have reacted with itself, either through cyclization or intermolecular reactions. The cyclized library was purified by Reverse Phase HPLC as previously described. Control experiments using uncyclized library showed complete incorporation of the fluorescent tags mentioned above.

Example 4

Preparation of Fluorescent Tags for Cyclization Assay

[0153] In separate tubes, propargyl glycine or 2-amino-3-phenylpropylazide (8 .mu.mol each) was combined with FAM-OSu (Molecular Probes Inc.) (1.2 equiv.) in pH 9.4 borate buffer (250 .mu.L). The reactions were allowed to proceed for 3 h at room temperature, and were then lyophilized overnight. Purification by HPLC afforded the desired fluorescent alkyne and azide in quantitative yield.

##STR00029##

Example 5

Cyclization of Individual Compounds Using the Azide/Alkyne Cycloaddition Reaction

Preparation of Azidoacetyl-Gly-Pro-Phe-Pra-NH.sub.2 (SEQ ID NO:895):

[0154] Using 0.3 mmol of Rink-amide resin, the indicated sequence was synthesized using standard solid phase synthesis techniques with Fmoc-protected amino acids and HATU as activating agent (Pra=C-propargylglycine). Azidoacetic acid was used to cap the tetrapeptide. The peptide was cleaved from the resin with 20% TFA/DCM for 4 h. Purification by RP HPLC afforded product as a white solid (75 mg, 51%). .sup.1H NMR (DMSO-d.sub.6, 400 MHz): 8.4-7.8 (m, 3H), 7.4-7.1 (m, 7H), 4.6-4.4 (m, 1H), 4.4-4.2 (m, 2H), 4.0-3.9 (m, 2H), 3.74 (dd, 1H, J=6 Hz, 17 Hz), 3.5-3.3 (m, 2H), 3.07 (dt, 1H, J=5 Hz, 14 Hz), 2.92 (dd, 1H, J=5 Hz, 16 Hz), 2.86 (t, 1H, J=2 Hz), 2.85-2.75 (m, 1H), 2.6-2.4 (m, 2H), 2.2-1.6 (m, 4H). IR (mull) 2900, 2100, 1450, 1300 cm.sup.-1. ESIMS 497.4 ([M+H], 100%), 993.4 ([2M+H], 50%). ESIMS with ion-source fragmentation: 519.3 ([M+Na], 100%), 491.3 (100%), 480.1 ([M-NH.sub.2], 90%), 452.2 ([M-NH.sub.2-CO], 20%), 424.2 (20%), 385.1 ([M-Pra], 50%), 357.1 ([M-Pra-CO], 40%), 238.0 ([M-Pra-Phe], 100%).

Cyclization of Azidoacetyl-Gly-Pro-Phe-Pra-NH.sub.2 (SEQ ID NO:895):

##STR00030##

[0156] The azidoacetyl peptide (31 mg, 0.62 mmol) was dissolved in MeCN (30 mL). Diisopropylethylamine (DIEA, 1 mL) and Cu(MeCN).sub.4 PF.sub.6 (1 mg) were added. After stirring for 1.5 h, the solution was evaporated and the resulting residue was taken up in 20% MeCN/H.sub.2O. After centrifugation to remove insoluble salts, the solution was subjected to preparative reverse phase HPLC. The desired cyclic peptide was isolated as a white solid (10 mg, 32%). .sup.1H NMR (DMSO-d.sub.6, 400 MHz): 8.28 (t, 1H, J=5 Hz), 7.77 (s, 1H), 7.2-6.9 (m, 9H), 4.98 (m, 2H), 4.48 (m, 1H), 4.28 (m, 1H), 4.1-3.9 (m, 2H), 3.63 (dd, 1H, J=5 Hz, 16 Hz), 3.33 (m, 2H), 3.0 (m, 3H), 2.48 (dd, 1H, J=11 Hz, 14 Hz), 1.75 (m, 1H0, 1.55 (m, 1H), 1.32 (m, 1H), 1.05 (m, 1H). IR (mull) 2900, 1475, 1400 cm.sup.-1. ESIMS 497.2 ([M+H], 100%), 993.2 ([2M+H], 30%), 1015.2 ([2M+Na], 15%). ESIMS with ion-source fragmentation: 535.2 (70%), 519.3 ([M+Na], 100%), 497.2 ([M+H], 80%), 480.1 ([M-NH.sub.2], 30%), 452.2 ([M-NH.sub.2-CO], 40%), 208.1 (60%).

Preparation of Azidoacetyl-Gly-Pro-Phe-Pra-Gly-OH (SEQ ID NO:895):

[0157] Using 0.3 mmol of Glycine-Wang resin, the indicated sequence was synthesized using Fmoc-protected amino acids and HATU as the activating agent. Azidoacetic acid was used in the last coupling step to cap the pentapeptide. Cleavage of the peptide was achieved using 50% TFA/DCM for 2 h. Purification by RP HPLC afforded the peptide as a white solid (83 mg; 50%). .sup.1H NMR (DMSO-d.sub.6, 400 MHz): 8.4-7.9 (m, 4H), 7.2 (m, 5H), 4.7-4.2 (m, 3H), 4.0-3.7 (m, 4H), 3.5-3.3 (m, 2H), 3.1 (m, 1H), 2.91 (dd, 1H, J=4 Hz, 16 Hz), 2.84 (t, 1H, J=2.5 Hz), 2.78 (m, 1H), 2.6-2.4 (m, 2H), 2.2-1.6 (m, 4H). IR (mull) 2900, 2100, 1450, 1350 cm.sup.-1. ESIMS 555.3 ([M+H], 100%). ESIMS with ion-source fragmentation: 577.1 ([M+Na], 90%), 555.3 ([M+H], 80%), 480.1 ([M-Gly], 100%), 385.1 ([M-Gly-Pra], 70%), 357.1 ([M-Gly-Pra-CO], 40%), 238.0 ([M-Gly-Pra-Phe], 80%).

Cyclization of Azidoacetyl-Gly-Pro-Phe-Pra-Gly-OH (SEQ ID NO:895):

[0158] The peptide (32 mg, 0.058 mmol) was dissolved in MeCN (60 mL). Diisopropylethylamine (1 mL) and Cu(MeCN).sub.4 PF.sub.6 (1 mg) were added and the solution was stirred for 2 h. The solvent was evaporated and the crude product was subjected to RP HPLC to remove dimers and trimers. The cyclic monomer was isolated as a colorless glass (6 mg, 20%). ESIMS 555.6 ([M+H], 100%), 1109.3 ([2M+H], 20%), 1131.2 ([2M+Na], 15%).

[0159] ESIMS with ion source fragmentation: 555.3 ([M+H], 100%), 480.4 ([M-Gly], 30%), 452.2 ([M-Gly-CO], 25%), 424.5 ([M-Gly-2CO], 10%, only possible in a cyclic structure).

Conjugation of Linear Peptide to DNA:

[0160] Compound 2 (45 nmol) was dissolved in 45 .mu.L sodium borate buffer (pH 9.4; 150 mM). At 4.degree. C., linear peptide (18 .mu.L of a 100 mM stock in DMF; 180 nmol; 40 equiv.) was added, followed by DMT-MM (3.6 .mu.L of a 500 mM stock in water; 180 nmol; 40 equiv.). After agitating for 2 h, LCMS showed complete reaction, and product was isolated by ethanol precipitation. ESIMS 1823.0 ([M-3H]/3, 20%), 1367.2 ([M-4H]/4, 20%), 1093.7 ([M-5H]/5, 40%), 911.4 ([M-6H]/6, 100%).

Conjugation of Cyclic Peptide to DNA:

[0161] Compound 2 (20 nmol) was dissolved in 20 .mu.L sodium borate buffer (pH 9.4, 150 mM). At 4.degree. C., linear peptide (8 .mu.L of a 100 mM stock in DMF; 80 nmol; 40 equiv.) was added, followed by DMT-MM (1.6 .mu.L of a 500 mM stock in water; 80 nmol; 40 equiv.). After agitating for 2 h, LCMS showed complete reaction, and product was isolated by ethanol precipitation. ESIMS 1823.0 ([M-3H]/3, 20%), 1367.2 ([M-4H]/4, 20%), 1093.7 ([M-5H]/5, 40%), 911.4 ([M-6H]/6, 100%).

Cyclization of DNA-Linked Peptide:

[0162] Linear peptide-DNA conjugate (10 nmol) was dissolved in pH 8 sodium phosphate buffer (10 .mu.L, 150 mm) At room temperature, 4 equivalents each of CuSO.sub.4, ascorbic acid, and the Sharpless ligand were all added (0.2 .mu.L of 200 mM stocks). The reaction was allowed to proceed overnight. RP HPLC showed that no linear peptide-DNA was present, and that the product co-eluted with authentic cyclic peptide-DNA. No traces of dimers or other oligomers were observed.

##STR00031##

Example 6

Application of Aromatic Nucleophilic Substitution Reactions to Functional Moiety Synthesis

[0163] General Procedure for Arylation of Compound 3 with Cyanuric Chloride:

[0164] Compound 2 is dissolved in pH 9.4 sodium borate buffer at a concentration of 1 mM. The solution is cooled to 4.degree. C. and 20 equivalents of cyanuric chloride is then added as a 500 mM solution in MeCN. After 2 h, complete reaction is confirmed by LCMS and the resulting dichlorotriazine-DNA conjugate is isolated by ethanol precipitation.

Procedure for Amine Substitution of Dichlorotriazine-DNA:

[0165] The dichlorotriazine-DNA conjugate is dissolved in pH 9.5 borate buffer at a concentration of 1 mM. At room temperature, 40 equivalents of an aliphatic amine is added as a DMF solution. The reaction is followed by LCMS and is usually complete after 2 h. The resulting alkylamino-monochlorotriazine-DNA conjugate is isolated by ethanol precipitation.

Procedure for Amine Substitution of Monochlorotriazine-DNA:

[0166] The alkylamino-monochlorotriazine-DNA conjugate is dissolved in pH 9.5 borate buffer at a concentration of 1 mM. At 42.degree. C., 40 equivalents of a second aliphatic amine is added as a DMF solution. The reaction is followed by LCMS and is usually complete after 2 h. The resulting diaminotriazine-DNA conjugate is isolated by ethanol precipitation.

Example 7

Application of Reductive Amination Reactions to Functional Moiety Synthesis

[0167] General Procedure for Reductive Amination of DNA-Linker Containing a Secondary Amine with an Aldehyde Building Block:

[0168] Compound 2 was coupled to an N-terminal proline residue. The resulting compound was dissolved in sodium phosphate buffer (50 .mu.L, 150 mM, pH 5.5) at a concentration of 1 mM. To this solution was added 40 equivalents each of an aldehyde building block in DMF (8 .mu.L, 0.25M) and sodium cyanoborohydride in DMF (8 .mu.L, 0.25M) and the solution was heated at 80.degree. C. for 2 hours. Following alkylation, the solution was purified by ethanol precipitation.

General Procedure for Reductive Aminations of DNA-Linker Containing an Aldehyde with Amine Building Blocks:

[0169] Compound 2 coupled to a building block comprising an aldehyde group was dissolved in sodium phosphate buffer (50 .mu.L, 250 mM, pH 5.5) at a concentration of 1 mM. To this solution was added 40 equivalents each of an amine building block in DMF (8 .mu.L, 0.25M) and sodium cyanoborohydride in DMF (8 .mu.L, 0.25M) and the solution was heated at 80.degree. C. for 2 hours. Following alkylation, the solution was purified by ethanol precipitation.

Example 8

Application of Peptoid Building Reactions to Functional Moiety Synthesis

General Procedure for Peptoid Synthesis on DNA-Linker:

##STR00032##

[0171] Compound 2 was dissolved in sodium borate buffer (50 .mu.L, 150 mM, pH 9.4) at a concentration of 1 mM and chilled to 4.degree. C. To this solution was added 40 equivalents of N-hydroxysuccinimidyl bromoacetate in DMF (13 .mu.L, 0.15 M) and the solution was gently shaken at 4.degree. C. for 2 hours. Following acylation, the DNA-Linker was purified by ethanol precipitation and redissolved in sodium borate buffer (50 .mu.L, 150 mM, pH 9.4) at a concentration of 1 mM and chilled to 4.degree. C. To this solution was added 40 equivalents of an amine building block in DMF (13 .mu.L, 0.15 M) and the solution was gently shaken at 4.degree. C. for 16 hours. Following alkylation, the DNA-linker was purified by ethanol precipitation and redissolved in sodium borate buffer (50 .mu.L, 150 mM, pH 9.4) at a concentration of 1 mM and chilled to 4.degree. C. Peptoid synthesis is continued by the stepwise addition of N-hydroxysuccinimidyl bromoacetate followed by the addition of an amine building block.

Example 9

Application of the Azide-Alkyne Cycloaddition Reaction to Functional Moiety Synthesis

General Procedure

[0172] An alkyne-containing DNA conjugate is dissolved in pH 8.0 phosphate buffer at a concentration of ca. 1 mM. To this mixture is added 10 equivalents of an organic azide and 5 equivalents each of copper (II) sulfate, ascorbic acid, and the ligand (tris-((1-benzyltriazol-4-yl)methyl)amine all at room temperature. The reaction is followed by LCMS, and is usually complete after 1-2 h. The resulting triazole-DNA conjugate can be isolated by ethanol precipitation.

Example 10

Identification of a Ligand to Abl Kinase from within an Encoded Library

[0173] The ability to enrich molecules of interest in a DNA-encoded library above undesirable library members is paramount to identifying single compounds with defined properties against therapeutic targets of interest. To demonstrate this enrichment ability a known binding molecule (described by Shah et al., Science 305, 399-401 (2004), incorporated herein by reference) to rhAbl kinase (GenBank U07563) was synthesized. This compound was attached to a double stranded DNA oligonucleotide via the linker described in the preceding examples using standard chemistry methods to produce a molecule similar (functional moiety linked to an oligonucleotide) to those produced via the methods described in Examples 1 and 2. A library generally produced as described in Example 2 and the DNA-linked Abl kinase binder were designed with unique DNA sequences that allowed qPCR analysis of both species. The DNA-linked Abl kinase binder was mixed with the library at a ratio of 1:1000. This mixture was equilibrated with to rhAble kinase, and the enzyme was captured on a solid phase, washed to remove non-binding library members and binding molecules were eluted. The ratio of library molecules to the DNA-linked Abl kinase inhibitor in the eluate was 1:1, indicating a greater than 500-fold enrichment of the DNA-linked Abl-kinase binder in a 1000-fold excess of library molecules.

EQUIVALENTS

[0174] Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Sequence CWU 1

1

90419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 1gcaacgaag 929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 2tcgttgcca 939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 3gcgtacaag 949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 4tgtacgcca 959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 5gctctgtag 969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 6acagagcca 979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 7gtgccatag 989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 8atggcacca 999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 9gttgaccag 9109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 10ggtcaacca 9119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 11cgacttgac 9129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 12acgctgaac 9139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 13cgtagtcag 9149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 14gactacgca 9159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 15ccagcatag 9169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 16atgctggca 9179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 17cctacagag 9189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 18ctgtaggca 9199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 19ctgaacgag 9209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 20acgacttgc 9219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 21ctccagtag 9229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 22actggagca 9239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 23taggtccag 9249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 24ggacctaca 9259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 25gcgtgttgt 9269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 26aacacgcct 9279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 27gcttggagt 9289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 28tccaagcct 9299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 29gtcaagcgt 9309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 30gcttgacct 9319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 31caagagcgt 9329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 32gctcttgct 9339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 33cagttcggt 9349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 34cgaactgct 9359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 35cgaaggagt 9369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 36tccttcgct 9379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 37cggtgttgt 9389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 38aacaccgct 9399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 39cgttgctgt 9409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 40agcaacgct 9419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 41ccgatctgt 9429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 42agatcggct 9439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 43ccttctcgt 9449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 44gagaaggct 9459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 45tgagtccgt 9469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 46ggactcact 9479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 47tgctacggt 9489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 48cgttagact 9499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 49gtgcgttga 9509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 50aacgcacac 9519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 51gttggcaga 9529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 52tgccaacac 9539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 53cctgtagga 9549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 54ctacaggac 9559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 55ctgcgtaga 9569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 56tacgcagac 9579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 57cttacgcga 9589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 58gcgtaagac 9599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 59tggtcacga 9609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 60gtgaccaac 9619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 61tcagagcga 9629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 62gctctgaac 9639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 63ttgctcgga 9649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 64cgagcaaac 9659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 65gcagttgga 9669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 66caactgcac 9679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 67gcctgaaga 9689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 68ttcaggcac 9699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 69gtagccaga 9709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 70tggctacac 9719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 71gtcgcttga 9729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 72aagcgacac 9739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 73gcctaagtt 9749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 74cttaggctc 9759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 75gtagtgctt 9769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 76gcactactc 9779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 77gtcgaagtt 9789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 78cttcgactc 9799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 79gtttcggtt 9809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 80ccgaaactc 9819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 81cagcgtttt 9829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 82aacgctgtc 9839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 83catacgctt 9849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 84gcgtatgtc 9859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 85cgatctgtt 9869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 86cagatcgtc 9879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 87cgctttgtt 9889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 88caaagcgtc 9899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 89ccacagttt 9909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 90actgtggtc 9919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 91cctgaagtt 9929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 92cttcaggtc 9939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 93ctgacgatt 9949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 94tcgtcagtc 9959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 95ctccacttt 9969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 96agtggagtc 9979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 97accagagcc 9989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 98ctctggtaa 9999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 99atccgcacc 91009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 100tgcggataa 91019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 101gacgacacc 91029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 102tgtcgtcaa 91039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 103ggatggacc 91049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 104tccatccaa 91059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 105gcagaagcc 91069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 106cttctgcaa 91079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 107gccatgtcc 91089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide

108acatggcaa 91099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 109gtctgctcc 91109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 110agcagacaa 91119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 111cgacagacc 91129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 112tctgtcgaa 91139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 113cgctactcc 91149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 114agtagcgaa 91159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 115ccacagacc 91169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 116tctgtggaa 91179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 117cctctctcc 91189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 118agagaggaa 91199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 119ctcgtagcc 91209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 120ctacgagaa 912120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 121aaatcgatgt ggtcactcag 2012220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 122gagtgaccac atcgatttgg 2012320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 123aaatcgatgt ggactaggag 2012420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 124cctagtccac atcgatttgg 2012520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 125aaatcgatgt gccgtatgag 2012620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 126catacggcac atcgatttgg 2012720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 127aaatcgatgt gctgaaggag 2012820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 128ccttcagcac atcgatttgg 2012920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 129aaatcgatgt ggactagcag 2013020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 130gctagtccac atcgatttgg 2013120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 131aaatcgatgt gcgctaagag 2013220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 132cttagcgcac atcgatttgg 2013320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 133aaatcgatgt gagccgagag 2013420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 134ctcggctcac atcgatttgg 2013520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 135aaatcgatgt gccgtatcag 2013620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 136gatacggcac atcgatttgg 2013720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 137aaatcgatgt gctgaagcag 2013820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 138gcttcagcac atcgatttgg 2013920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 139aaatcgatgt gtgcgagtag 2014020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 140actcgcacac atcgatttgg 2014120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 141aaatcgatgt gtttggcgag 2014220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 142cgccaaacac atcgatttgg 2014320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 143aaatcgatgt gcgctaacag 2014420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 144gttagcgcac atcgatttgg 2014520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 145aaatcgatgt gagccgacag 2014620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 146gtcggctcac atcgatttgg 2014720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 147aaatcgatgt gagccgaaag 2014820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 148ttcggctcac atcgatttgg 2014920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 149aaatcgatgt gtcggtagag 2015020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 150ctaccgacac atcgatttgg 2015120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 151aaatcgatgt ggttgccgag 2015220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 152cggcaaccac atcgatttgg 2015320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 153aaatcgatgt gagtgcgtag 2015420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 154acgcactcac atcgatttgg 2015520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 155aaatcgatgt ggttgccaag 2015620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 156tggcaaccac atcgatttgg 2015720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 157aaatcgatgt gtgcgaggag 2015820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 158cctcgcacac atcgatttgg 2015920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 159aaatcgatgt ggaacacgag 2016020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 160cgtgttccac atcgatttgg 2016120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 161aaatcgatgt gcttgtcgag 2016220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 162cgacaagcac atcgatttgg 2016320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 163aaatcgatgt gttccggtag 2016420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 164accggaacac atcgatttgg 2016520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 165aaatcgatgt gtgcgagcag 2016620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 166gctcgcacac atcgatttgg 2016720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 167aaatcgatgt ggtcaggtag 2016820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 168acctgaccac atcgatttgg 2016920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 169aaatcgatgt ggcctgttag 2017020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 170aacaggccac atcgatttgg 2017120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 171aaatcgatgt ggaacaccag 2017220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 172ggtgttccac atcgatttgg 2017320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 173aaatcgatgt gcttgtccag 2017420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 174ggacaagcac atcgatttgg 2017520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 175aaatcgatgt gtgcgagaag 2017620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 176tctcgcacac atcgatttgg 2017720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 177aaatcgatgt gagtgcggag 2017820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 178ccgcactcac atcgatttgg 2017920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 179aaatcgatgt gttgtccgag 2018020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 180cggacaacac atcgatttgg 2018120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 181aaatcgatgt gtggaacgag 2018220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 182cgttccacac atcgatttgg 2018320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 183aaatcgatgt gagtgcgaag 2018420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 184tcgcactcac atcgatttgg 2018520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 185aaatcgatgt gtggaaccag 2018620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 186ggttccacac atcgatttgg 2018720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 187aaatcgatgt gttaggcgag 2018820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 188cgcctaacac atcgatttgg 2018920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 189aaatcgatgt ggcctgtgag 2019020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 190cacaggccac atcgatttgg 2019120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 191aaatcgatgt gctcctgtag 2019220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 192acaggagcac atcgatttgg 2019320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 193aaatcgatgt ggtcaggcag 2019420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 194gcctgaccac atcgatttgg 2019520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 195aaatcgatgt ggtcaggaag 2019620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 196tcctgaccac atcgatttgg 2019720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 197aaatcgatgt ggtagccgag 2019820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 198cggctaccac atcgatttgg 2019920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 199aaatcgatgt ggcctgtaag 2020020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 200tacaggccac atcgatttgg 2020120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 201aaatcgatgt gctttcggag 2020220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 202ccgaaagcac atcgatttgg 2020320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 203aaatcgatgt gcgtaaggag 2020420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 204ccttacgcac atcgatttgg 2020520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 205aaatcgatgt gagagcgtag 2020620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 206acgctctcac atcgatttgg 2020720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 207aaatcgatgt ggacggcaag 2020820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 208tgccgtccac atcgatttgg 2020920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 209aaatcgatgt gctttcgcag

2021020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 210gcgaaagcac atcgatttgg 2021120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 211aaatcgatgt gcgtaagcag 2021220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 212gcttacgcac atcgatttgg 2021320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 213aaatcgatgt ggctatggag 2021420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 214ccatagccac atcgatttgg 2021520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 215aaatcgatgt gactctggag 2021620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 216ccagagtcac atcgatttgg 2021719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 217aaatcgatgt gctggaaag 1921819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 218ttccagcaca tcgatttgg 1921920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 219aaatcgatgt gccgaagtag 2022020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 220acttcggcac atcgatttgg 2022120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 221aaatcgatgt gctcctgaag 2022220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 222tcaggagcac atcgatttgg 2022320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 223aaatcgatgt gtccagtcag 2022420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 224gactggacac atcgatttgg 2022520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 225aaatcgatgt gagagcggag 2022620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 226ccgctctcac atcgatttgg 2022720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 227aaatcgatgt gagagcgaag 2022820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 228tcgctctcac atcgatttgg 2022920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 229aaatcgatgt gccgaaggag 2023020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 230ccttcggcac atcgatttgg 2023120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 231aaatcgatgt gccgaagcag 2023220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 232gcttcggcac atcgatttgg 2023320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 233aaatcgatgt gtgttccgag 2023420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 234cggaacacac atcgatttgg 2023520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 235aaatcgatgt gtctggcgag 2023620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 236cgccagacac atcgatttgg 2023720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 237aaatcgatgt gctatcggag 2023820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 238ccgatagcac atcgatttgg 2023920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 239aaatcgatgt gcgaaaggag 2024020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 240cctttcgcac atcgatttgg 2024120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 241aaatcgatgt gccgaagaag 2024220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 242tcttcggcac atcgatttgg 2024320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 243aaatcgatgt ggttgcagag 2024420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 244ctgcaaccac atcgatttgg 2024520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 245aaatcgatgt ggatggtgag 2024620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 246caccatccac atcgatttgg 2024720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 247aaatcgatgt gctatcgcag 2024820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 248gcgatagcac atcgatttgg 2024920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 249aaatcgatgt gcgaaagcag 2025020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 250gctttcgcac atcgatttgg 2025120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 251aaatcgatgt gacactggag 2025220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 252ccagtgtcac atcgatttgg 2025320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 253aaatcgatgt gtctggcaag 2025420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 254tgccagacac atcgatttgg 2025520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 255aaatcgatgt ggatggtcag 2025620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 256gaccatccac atcgatttgg 2025720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 257aaatcgatgt ggttgcacag 2025820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 258gtgcaaccac atcgatttgg 2025920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 259aaatcgatgt gggcatcgag 2026020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 260cgatgcccca tccgatttgg 2026120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 261aaatcgatgt gtgcctccag 2026220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 262ggaggcacac atcgatttgg 2026320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 263aaatcgatgt gtgcctcaag 2026420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 264tgaggcacac atcgatttgg 2026520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 265aaatcgatgt gggcatccag 2026620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 266ggatgcccac atcgatttgg 2026720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 267aaatcgatgt gggcatcaag 2026820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 268tgatgcccac atcgatttgg 2026920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 269aaatcgatgt gcctgtcgag 2027020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 270cgacaggcac atcgatttgg 2027120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 271aaatcgatgt ggacggatag 2027220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 272atccgtccac atcgatttgg 2027320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 273aaatcgatgt gcctgtccag 2027420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 274ggacaggcac atcgatttgg 2027520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 275aaatcgatgt gaagcacgag 2027620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 276cgtgcttcac atcgatttgg 2027720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 277aaatcgatgt gcctgtcaag 2027820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 278tgacaggcac atcgatttgg 2027920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 279aaatcgatgt gaagcaccag 2028020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 280ggtgcttcac atcgatttgg 2028120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 281aaatcgatgt gccttcgtag 2028220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 282acgaaggcac atcgatttgg 2028320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 283aaatcgatgt gtcgtccgag 2028420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 284cggacgacac atcgatttgg 2028520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 285aaatcgatgt ggagtctgag 2028620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 286cagactccac atcgatttgg 2028720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 287aaatcgatgt gtgatccgag 2028820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 288cggatcacac atcgatttgg 2028920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 289aaatcgatgt gtcaggcgag 2029020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 290cgcctgacac atcgatttgg 2029120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 291aaatcgatgt gtcgtccaag 2029220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 292tggacgacac atcgatttgg 2029320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 293aaatcgatgt ggacggagag 2029420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 294ctccgtccac atcgatttgg 2029520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 295aaatcgatgt ggtagcagag 2029620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 296ctgctaccac atcgatttgg 2029720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 297aaatcgatgt ggctgtgtag 2029820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 298acacagccac atcgatttgg 2029920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 299aaatcgatgt ggacggacag 2030020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 300gtccgtccac atcgatttgg 2030120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 301aaatcgatgt gtcaggcaag 2030220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 302tgcctgacac atcgatttgg 2030320DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 303aaatcgatgt ggctcgaaag 2030420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 304ttcgagccac atcgatttgg 2030520DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 305aaatcgatgt gccttcggag 2030620DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 306ccgaaggcac atcgatttgg 2030720DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 307aaatcgatgt ggtagcacag 2030820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 308gtgctaccac atcgatttgg 2030920DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 309aaatcgatgt ggaaggtcag 2031020DNAArtificial SequenceDescription of Artificial Sequence Synthetic

oligonucleotide 310gaccttccac atcgatttgg 2031120DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 311aaatcgatgt ggtgctgtag 2031220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 312acagcaccac atcgatttgg 203139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 313gttgcctgt 93149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 314aggcaacct 93159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 315caggacggt 93169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 316cgtcctgct 93179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 317agacgtggt 93189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 318cacgtctct 93199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 319caggaccgt 93209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 320ggtcctgct 93219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 321caggacagt 93229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 322tgtcctgct 93239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 323cactctggt 93249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 324cagagtgct 93259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 325gacggctgt 93269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 326agccgtcct 93279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 327cactctcgt 93289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 328gagagtgct 93299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 329gtagcctgt 93309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 330aggctacct 93319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 331gccacttgt 93329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 332aagtggcct 93339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 333catcgctgt 93349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 334agcgatgct 93359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 335cactggtgt 93369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 336accagtgct 93379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 337gccactggt 93389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 338cagtggcct 93399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 339tctggctgt 93409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 340agccagact 93419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 341gccactcgt 93429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 342gagtggcct 93439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 343tgcctctgt 93449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 344agaggcact 93459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 345catcgcagt 93469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 346tgcgatgct 93479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 347caggaaggt 93489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 348cttcctgct 93499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 349ggcatctgt 93509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 350agatgccct 93519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 351cggtgctgt 93529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 352agcaccgct 93539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 353cactggcgt 93549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 354gccagtgct 93559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 355tctcctcgt 93569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 356gaggagact 93579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 357cctgtctgt 93589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 358agacaggct 93599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 359caacgctgt 93609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 360agcgttgct 93619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 361tgcctcggt 93629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 362cgaggcact 93639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 363acactgcgt 93649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 364gcagtgtct 93659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 365tcgtcctgt 93669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 366aggacgact 93679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 367gctgccagt 93689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 368tggcagcct 93699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 369tcaggctgt 93709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 370agcctgact 93719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 371gccaggtgt 93729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 372acctggcct 93739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 373cggacctgt 93749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 374aggtccgct 93759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 375caacgcagt 93769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 376tgcgttgct 93779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 377cacacgagt 93789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 378tcgtgtgct 93799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 379atggcctgt 93809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 380aggccatct 93819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 381ccagtctgt 93829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 382agactggct 93839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 383gccaggagt 93849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 384tcctggcct 93859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 385cggaccagt 93869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 386tggtccgct 93879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 387ccttcgcgt 93889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 388gcgaaggct 93899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 389gcagccagt 93909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 390tggctgcct 93919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 391ccagtcggt 93929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 392cgactggct 93939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 393actgagcgt 93949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 394gctcagtct 93959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 395ccagtccgt 93969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 396ggactggct 93979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 397ccagtcagt 93989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 398tgactggct 93999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 399catcgaggt 94009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 400ctcgatgct 94019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 401ccatcgtgt 94029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 402acgatggct 94039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 403gtgctgcgt 94049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 404gcagcacct 94059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 405gactacggt 94069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 406cgtagtcct 94079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 407gtgctgagt 94089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 408tcagcacct 94099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 409gctgcatgt 94109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 410atgcagcct 94119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 411gagtggtgt 94129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 412accactcct 94139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 413gactaccgt 94149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 414ggtagtcct 94159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 415cggtgatgt 94169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 416atcaccgct 94179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 417tgcgactgt

94189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 418agtcgcact 94199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 419tctggaggt 94209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 420ctccagact 94219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 421agcactggt 94229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 422cagtgctct 94239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 423tcgcttggt 94249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 424caagcgact 94259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 425agcactcgt 94269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 426gagtgctct 94279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 427gcgattggt 94289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 428caatcgcct 94299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 429ccatcgcgt 94309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 430gcgatggct 94319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 431tcgcttcgt 94329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 432gaagcgact 94339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 433agtgcctgt 94349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 434aggcactct 94359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 435ggcataggt 94369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 436ctatgccct 94379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 437gcgattcgt 94389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 438gaatcgcct 94399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 439tgcgacggt 94409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 440cgtcgcact 94419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 441gagtggcgt 94429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 442gccactcct 94439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 443cggtgaggt 94449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 444ctcaccgct 94459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 445gctgcaagt 94469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 446ttgcagcct 94479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 447ttccgctgt 94489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 448agcggaact 94499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 449gagtggagt 94509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 450tccactcct 94519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 451acagagcgt 94529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 452gctctgtct 94539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 453tgcgaccgt 94549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 454ggtcgcact 94559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 455cctgtaggt 94569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 456ctacaggct 94579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 457tagccgtgt 94589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 458acggctact 94599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 459tgcgacagt 94609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 460tgtcgcact 94619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 461ggtctgtgt 94629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 462acagaccct 94639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 463cggtgaagt 94649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 464ttcaccgct 94659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 465caacgaggt 94669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 466ctcgttgct 94679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 467gcagcatgt 94689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 468atgctgcct 94699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 469tcgtcaggt 94709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 470ctgacgact 94719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 471agtgccagt 94729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 472tggcactct 94739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 473tagaggcgt 94749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 474gcctctact 94759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 475gtcagcggt 94769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 476cgctgacct 94779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 477tcaggaggt 94789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 478ctcctgact 94799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 479agcaggtgt 94809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 480acctgctct 94819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 481ttccgcagt 94829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 482tgcggaact 94839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 483gtcagccgt 94849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 484ggctgacct 94859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 485ggtctgcgt 94869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 486gcagaccct 94879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 487tagccgagt 94889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 488tcggctact 94899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 489gtcagcagt 94909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 490tgctgacct 94919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 491ggtctgagt 94929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 492tcagaccct 94939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 493cggacaggt 94949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 494ctgtccgct 94959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 495ttagccggt 94969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 496cggctaact 94979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 497gagacgagt 94989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 498tcgtctcct 94999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 499cgtaaccgt 95009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 500ggttacgct 95019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 501ttggcgtgt 95029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 502acgccaact 95039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 503atggcaggt 95049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 504ctgccatct 95059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 505cagctacga 95069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 506gtagctgac 95079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 507ctcctgcga 95089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 508gcaggagac 95099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 509gctgcctga 95109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 510aggcagcac 95119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 511caggaacga 95129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 512gttcctgac 95139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 513cacacgcga 95149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 514gcgtgtgac 95159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 515gcagcctga 95169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 516aggctgcac 95179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 517ctgaacgga 95189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 518cgttcagac 95199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 519ctgaaccga 95209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 520ggttcagac 95219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 521tctggacga 95229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 522gtccagaac 95239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 523tgcctacga 95249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 524gtaggcaac 95259DNAArtificial SequenceDescription of Artificial Sequence Synthetic

oligonucleotide 525ggcatacga 95269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 526gtatgccac 95279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 527cggtgacga 95289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 528gtcaccgac 95299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 529caacgacga 95309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 530gtcgttgac 95319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 531ctcctctga 95329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 532agaggagac 95339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 533tcaggacga 95349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 534gtcctgaac 95359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 535aaaggcgga 95369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 536cgcctttac 95379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 537ctcctcgga 95389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 538cgaggagac 95399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 539cagatgcga 95409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 540gcatctgac 95419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 541gcagcaaga 95429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 542ttgctgcac 95439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 543gtggagtga 95449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 544actccacac 95459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 545ccagtagga 95469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 546ctactggac 95479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 547atggcacga 95489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 548gtgccatac 95499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 549ggactgtga 95509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 550acagtccac 95519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 551ccgaactga 95529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 552agttcggac 95539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 553ctcctcaga 95549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 554tgaggagac 95559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 555cactgctga 95569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 556agcagtgac 95579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 557agcaggcga 95589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 558gcctgctac 95599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 559agcaggaga 95609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 560tcctgctac 95619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 561agagccaga 95629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 562tggctctac 95639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 563gtcgttgga 95649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 564caacgacac 95659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 565ccgaacgga 95669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 566cgttcggac 95679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 567cactgcgga 95689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 568cgcagtgac 95699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 569gtggagcga 95709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 570gctccacac 95719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 571gtggagaga 95729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 572tctccacac 95739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 573ggactgcga 95749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 574gcagtccac 95759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 575ccgaaccga 95769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 576ggttcggac 95779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 577cactgccga 95789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 578ggcagtgac 95799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 579cgaaacgga 95809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 580cgtttcgac 95819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 581ggactgaga 95829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 582tcagtccac 95839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 583ccgaacaga 95849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 584tgttcggac 95859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 585cgaaaccga 95869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 586ggtttcgac 95879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 587ctggcttga 95889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 588aagccagac 95899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 589cacacctga 95909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 590aggtgtgac 95919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 591aacgaccga 95929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 592ggtcgttac 95939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 593atccagcga 95949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 594gctggatac 95959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 595tgcgaagga 95969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 596cttcgcaac 95979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 597tgcgaacga 95989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 598gttcgcaac 95999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 599ctggctgga 96009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 600cagccagac 96019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 601cacaccgga 96029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 602cggtgtgac 96039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 603agtgcagga 96049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 604ctgcactac 96059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 605gaccgttga 96069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 606aacggtcac 96079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 607ggtgagtga 96089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 608actcaccac 96099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 609ccttcctga 96109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 610aggaaggac 96119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 611ctggctaga 96129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 612tagccagac 96139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 613cacaccaga 96149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 614tggtgtgac 96159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 615agcggtaga 96169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 616taccgctac 96179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 617gtcagagga 96189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 618ctctgacac 96199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 619ttccgacga 96209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 620gtcggaaac 96219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 621aggcgtaga 96229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 622tacgcctac 96239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 623ctcgactga 96249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 624agtcgagac 96259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 625tacgctgga 96269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 626cagcgtaac 96279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 627gttcggtga 96289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 628accgaacac 96299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 629gccagcaga 96309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 630tgctggcac 96319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 631gaccgtaga 96329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 632tacggtcac

96339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 633gtgctctga 96349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 634agagcacac 96359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 635ggtgagcga 96369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 636gctcaccac 96379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 637ggtgagaga 96389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 638tctcaccac 96399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 639ccttccaga 96409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 640tggaaggac 96419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 641ctcctacga 96429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 642gtaggagac 96439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 643ctcgacgga 96449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 644cgtcgagac 96459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 645gccgtttga 96469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 646aaacggcac 96479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 647gcggagtga 96489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 648actccgcac 96499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 649cgtgcttga 96509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 650aagcacgac 96519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 651ctcgaccga 96529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 652ggtcgagac 96539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 653agagcagga 96549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 654ctgctctac 96559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 655gtgctcgga 96569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 656cgagcacac 96579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 657ctcgacaga 96589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 658tgtcgagac 96599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 659ggagagtga 96609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 660actctccac 96619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 661aggctgtga 96629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 662acagcctac 96639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 663agagcacga 96649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 664gtgctctac 96659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 665ccatcctga 96669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 666aggatggac 96679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 667gttcggaga 96689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 668tccgaacac 96699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 669tggtagcga 96709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 670gctaccaac 96719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 671gtgctccga 96729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 672ggagcacac 96739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 673gtgctcaga 96749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 674tgagcacac 96759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 675gccgttgga 96769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 676caacggcac 96779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 677gagtgctga 96789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 678agcactcac 96799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 679gctccttga 96809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 680aaggagcac 96819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 681ccgaaagga 96829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 682ctttcggac 96839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 683cactgagga 96849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 684ctcagtgac 96859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 685cgtgctgga 96869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 686cagcacgac 96879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 687ccgaaacga 96889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 688gtttcggac 96899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 689gcggagaga 96909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 690tctccgcac 96919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 691gccgttaga 96929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 692taacggcac 96939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 693tctcgtgga 96949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 694cacgagaac 96959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 695cgtgctaga 96969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 696tagcacgac 96979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 697gcctgtctt 96989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 698gacaggctc 96999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 699ctcctggtt 97009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 700ccaggagtc 97019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 701actctgctt 97029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 702gcagagttc 97039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 703catcgcctt 97049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 704ggcgatgtc 97059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 705gccactatt 97069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 706tagtggctc 97079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 707cacacggtt 97089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 708ccgtgtgtc 97099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 709caacgcctt 97109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 710ggcgttgtc 97119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 711actgaggtt 97129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 712cctcagttc 97139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 713gtgctggtt 97149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 714ccagcactc 97159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 715catcgactt 97169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 716gtcgatgtc 97179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 717ccatcggtt 97189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 718ccgatggtc 97199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 719gctgcactt 97209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 720gtgcagctc 97219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 721acagaggtt 97229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 722cctctgttc 97239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 723agtgccgtt 97249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 724cggcacttc 97259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 725cggacattt 97269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 726atgtccgtc 97279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 727ggtctggtt 97289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 728ccagacctc 97299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 729gagacggtt 97309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 730ccgtctctc 97319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 731ctttccgtt 97329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 732cggaaagtc 97339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 733cagatggtt 97349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 734ccatctgtc 97359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 735cggacactt 97369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 736gtgtccgtc 97379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 737actctcgtt 97389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 738cgagagttc 97399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 739gcagcactt 97409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide

740gtgctgctc 97419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 741actctcctt 97429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 742ggagagttc 97439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 743accttggtt 97449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 744ccaaggttc 97459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 745agagccgtt 97469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 746cggctcttc 97479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 747accttgctt 97489DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 748gcaaggttc 97499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 749aagtccgtt 97509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 750cggactttc 97519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 751ggactggtt 97529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 752ccagtcctc 97539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 753gtcgttctt 97549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 754gaacgactc 97559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 755cagcatctt 97569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 756gatgctgtc 97579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 757ctatccgtt 97589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 758cggatagtc 97599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 759acactcgtt 97609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 760cgagtgttc 97619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 761atccaggtt 97629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 762cctggattc 97639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 763gttcctgtt 97649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 764caggaactc 97659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 765acactcctt 97669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 766ggagtgttc 97679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 767gttcctctt 97689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 768gaggaactc 97699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 769ctggctctt 97709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 770gagccagtc 97719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 771acggcattt 97729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 772atgccgttc 97739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 773ggtgaggtt 97749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 774cctcacctc 97759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 775ccttccgtt 97769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 776cggaaggtc 97779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 777tacgctctt 97789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 778gagcgtatc 97799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 779acggcagtt 97809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 780ctgccgttc 97819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 781actgacgtt 97829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 782cgtcagttc 97839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 783acggcactt 97849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 784gtgccgttc 97859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 785actgacctt 97869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 786ggtcagttc 97879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 787tttgcggtt 97889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 788ccgcaaatc 97899DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 789tggtaggtt 97909DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 790cctaccatc 97919DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 791gttcggctt 97929DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 792gccgaactc 97939DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 793gccgttctt 97949DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 794gaacggctc 97959DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 795ggagaggtt 97969DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 796cctctcctc 97979DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 797cactgactt 97989DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 798gtcagtgtc 97999DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 799cgtgctctt 98009DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 800gagcacgtc 98019DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 801aatccgctt 98029DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 802gcggatttc 98039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 803aggctggtt 98049DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 804ccagccttc 98059DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 805gctagtgtt 98069DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 806cactagctc 98079DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 807ggagagctt 98089DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 808gctctcctc 98099DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 809ggagagatt 98109DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 810tctctcctc 98119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 811aggctgctt 98129DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 812gcagccttc 98139DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 813gagtgcgtt 98149DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 814cgcactctc 98159DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 815ccatccatt 98169DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 816tggatggtc 98179DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 817gctagtctt 98189DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 818gactagctc 98199DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 819aggctgatt 98209DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 820tcagccttc 98219DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 821acagacgtt 98229DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 822cgtctgttc 98239DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 823gagtgcctt 98249DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 824ggcactctc 98259DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 825acagacctt 98269DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 826ggtctgttc 98279DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 827cgagctttt 98289DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 828aagctcgtc 98299DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 829ttagcggtt 98309DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 830ccgctaatc 98319DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 831cctcttgtt 98329DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 832caagaggtc 98339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 833ggtctcttt 98349DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 834agagacctc 98359DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 835gccagattt 98369DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 836atctggctc 98379DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 837gagaccttt 98389DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 838aggtctctc 98399DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 839cacacagtt 98409DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 840ctgtgtgtc 98419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 841cctcttctt 98429DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 842gaagaggtc 98439DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 843tagagcgtt 98449DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 844cgctctatc 98459DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 845gcacctttt 98469DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 846aaggtgctc 98479DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 847ggcttgttt 98489DNAArtificial

SequenceDescription of Artificial Sequence Synthetic oligonucleotide 848acaagcctc 98499DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 849gacgcgatt 98509DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 850tcgcgtctc 98519DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 851cgagctgtt 98529DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 852cagctcgtc 98539DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 853tagagcctt 98549DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 854ggctctatc 98559DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 855catccgttt 98569DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 856acggatgtc 98579DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 857ggtctcgtt 98589DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 858cgagacctc 98599DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 859gccagagtt 98609DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 860ctctggctc 98619DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 861gagaccgtt 98629DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 862cggtctctc 98639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 863cgagctatt 98649DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 864tagctcgtc 98659DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 865gcaagtgtt 98669DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 866cacttgctc 98679DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 867ggtctcctt 98689DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 868ggagacctc 98699DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 869gccagactt 98709DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 870gtctggctc 98719DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 871ggtctcatt 98729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 872tgagacctc 98739DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 873gagaccatt 98749DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 874tggtctctc 98759DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 875ccttcagtt 98769DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 876ctgaaggtc 98779DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 877gcacctgtt 98789DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 878caggtgctc 98799DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 879aaaggcgtt 98809DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 880cgccttttc 98819DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 881cagatcgtt 98829DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 882cgatctgtc 98839DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 883cataggctt 98849DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 884gcctatgtc 98859DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 885ccttcactt 98869DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 886gtgaaggtc 98879DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 887gcacctctt 98889DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 888gaggtgctc 988925DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 889cagaagacag acaagcttca cctgc 2589027DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 890gcaggtgaag cttgtctgtc ttctgaa 2789119DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 891tgactcccaa atcaatgtg 1989217DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 892cattgatttg ggagtca 1789321DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 893ggcacattga tttgggagtc a 2189419DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 894tgactcccaa atcaatgtg 198955PRTArtificial SequenceDescription of Artificial Sequence Synthetic peptide 895Gly Pro Phe Xaa Gly1 589625DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 896agtctggtac agggtgttct tttta 2589729DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 897accgtaaaaa gaacaccctg taccagact 2989812DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 898cggtggctgg ag 1289912DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 899aaggctccag cc 1290037DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 900agtctggtac agggtgttct ttttacggtg gctggag 3790141DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 901aaggctccag ccaccgtaaa aagaacaccc tgtaccagac t 4190231DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 902cggaaacggg taccctaaaa agaacaccct g 3190339DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 903agtctggtac aggtgttctt tttagggtac ccgtttccg 3990440DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 904cggaaacggg taccctaaaa agaacaccct gtaccagact 40

Claims

1. A method of synthesizing a library of compounds, wherein the compounds comprise a functional moiety comprising two or more building blocks which is operatively linked to an initial oligonucleotide which identifies the structure of the functional moiety, said method comprising the steps of (a) providing a solution comprising m initiator compounds, wherein m is an integer of 1 or greater, where the initiator compounds consist of a functional moiety comprising n building blocks, where n is an integer of 1 or greater, which is operatively linked to an initial oligonucleotide which identifies the n building blocks; (b) dividing the solution of step (a) into r reaction vessels, wherein r is an integer of 2 or greater, thereby producing r aliquots of the solution; (c) reacting the initiator compounds in each reaction vessel with one of r building blocks, thereby producing r aliquots comprising compounds consisting of a functional moiety comprising n+1 building blocks operatively linked to the initial oligonucleotide; and (d) reacting the initial oligonucleotide in each aliquot with one of a set of r distinct incoming oligonucleotides in the presence of an enzyme which catalyzes the ligation of the incoming oligonucleotide and the initial oligonucleotide, under conditions suitable for enzymatic ligation of the incoming oligonucleotide and the initial oligonucleotide; thereby producing r aliquots comprising molecules consisting of a functional moiety comprising n+1 building blocks operatively linked to an elongated oligonucleotide which encodes the n+1 building blocks.

2. The method of claim 1, further comprising the step of (e) combining two or more of the r aliquots, thereby producing a solution comprising molecules consisting of a functional moiety comprising n+1 building blocks, which is operatively linked to an elongated oligonucleotide which encodes the n+1 building blocks.

3. The method of claim 2 wherein r aliquots are combined.

4. The method of claim 2 wherein the steps (a) to (e) are conducted one or more times to yield cycles 1 to i, where i is an integer of 2 or greater, wherein in cycle s+1, where s is an integer of i-1 or less, the solution comprising m initiator compounds of step (a) is the solution of step (e) of cycle s.

5. The method of either claim 1 or claim 2 wherein in at least one of cycles 1 to step (d) precedes step (c).

6. The method of claim 2 wherein at least one of building blocks is an amino acid.

7. The method of claim 1, wherein the enzyme is DNA ligase, RNA ligase, DNA polymerase, RNA polymerase or topoisomerase.

8. The method of claim 2 wherein the initial oligonucleotide is a double-stranded oligonucleotide.

9. The method of claim 8 wherein the incoming oligonucleotide is a double-stranded oligonucleotide.

10. The method of claim 2 wherein the initiator compounds comprise a linker moiety comprising a first functional group adapted to bond with a building block, a second functional group adapted to bond to the 5' end of an oligonucleotide, and a third functional group adapted to bond to the 3'-end of an oligonucleotide.

11. The method of claim 10 wherein the linker moiety is of the structure ##STR00033## wherein A is a functional group adapted to bond to a building block; B is a functional group adapted to bond to the 5'-end of an oligonucleotide; C is a functional group adapted to bond to the 3'-end of an oligonucleotide; S is an atom or a scaffold; D is a chemical structure that connects A to S; E is a chemical structure that connects B to S; and F is a chemical structure that connects C to S.

12. The method of claim 11 wherein: A is an amino group; B is a phosphate group; and C is a phosphate group.

13. The method of claim 11 wherein D, E and F are each, independently, an alkylene group or an oligo(ethylene glycol) group.

14. The method of claim 11 wherein S is a carbon atom, a nitrogen atom, a phosphorus atom, a boron atom, a phosphate group, a cyclic group or a polycyclic group.

15. The method of claim 14 wherein the linker moiety is of the structure ##STR00034## wherein each of n, m and p is, independently, an integer from 1 to about 20.

16. The method of claim 15 wherein each of n, m and p is independently an integer from 2 to eight.

17. The method of claim 16 wherein each of n, m and p is independently an integer from 3 to 6.

18. The method of claim 15 wherein the linker moiety has the structure ##STR00035##

19. The method of claim 1, wherein each of said initiator compounds comprises a reactive group and wherein each of said r building blocks comprises a complementary reactive group which is complementary to said reactive group.

20. The method of claim 19 wherein the reactive group and the complementary reactive group are selected from the group consisting of an amino group; a carboxyl group; a sulfonyl group; a phosphonyl group; an epoxide group; an aziridine group; and an isocyanate group.

21. The method of claim 19 wherein reactive group and the complementary reactive group are selected from the group consisting of a hydroxyl group; a carboxyl group; a sulfonyl group; a phosphonyl group; an epoxide group; an aziridine group; and an isocyanate group.

22. The method of claim 19 wherein the reactive group and the complementary reactive group are selected from the group consisting of an amino group and an aldehyde or ketone group.

23. The method of claim 19 wherein the reaction between the reactive group and the complementary reactive group is conducted under reducing conditions.

24. The method of claim 19 wherein the reactive group and the complementary reactive group are selected from the group consisting of a phosphorous ylide group and an aldehyde or ketone group.

25. The method of claim 19 wherein the reactive group and the complementary reactive group react via cycloaddition to form a cyclic structure.

26. The method of claim 25 wherein the reactive group and the complementary reactive group are selected from the group consisting of an alkyne and an azide.

27. The method of claim 19 wherein the reactive group and the complementary functional group are selected from the group consisting of a halogenated heteroaromatic group and a nucleophile.

28. The method of claim 27 wherein the halogenated heteroaromatic group is selected from the group consisting of chlorinated pyrimidines, chlorinated triazines and chlorinated purines.

29. The method of claim 27 wherein the nucleophile is an amino group.

30. The method of claim 2, further comprising following cycle i, the step of: (f) cyclizing one or more of the functional moieties.

31. The method of claim 30 wherein a functional moiety of step (f) comprises an azido group and an alkynyl group.

32. The method of claim 31 wherein the functional moiety is maintained under conditions suitable for cycloaddition of the azido group and the alkynyl group to form a triazole group, thereby forming a cyclic functional moiety

33. The method of claim 32 wherein the cycloaddition reaction is conducted in the presence of a copper catalyst.

34. The method of claim 33 wherein at least one of the one or more functional moieties of step (f) comprises at least two sulfhydryl groups, and said functional moiety is maintained under conditions suitable for reaction of the two sulfhydryl groups to form a disulfide group, thereby cyclicizing the functional moiety.

35. The method of claim 1 wherein the initial oligonucleotide comprises a PCR primer sequence.

36. The method of claim 2, wherein the incoming oligonucleotide of cycle i comprises a PCR closing primer.

37. The method of claim 2, further comprising following cycle i, the step of (d) ligating an oligonucleotide comprising a closing PCR primer sequence to the encoding oligonucleotide.

38. The method of claim 37 wherein the oligonucleotide comprising a closing PCR primer sequence is ligated to the encoding oligonucleotide in the presence of an enzyme which catalyzes said ligation.