U.S. patent application number 12/699979 was filed with the patent office on 2011-02-24 for protein variants having modified immunogenicity.
This patent application is currently assigned to NOVOZYMES A/S. Invention is credited to Steffen Ernst, Esben Peter Friis, Erwin Ludo Roggen, Allan Svendsen, Claus Von Der Osten.
Application Number | 20110045572 12/699979 |
Document ID | / |
Family ID | 26068820 |
Filed Date | 2011-02-24 |
United States Patent
Application |
20110045572 |
Kind Code |
A1 |
Roggen; Erwin Ludo ; et
al. |
February 24, 2011 |
PROTEIN VARIANTS HAVING MODIFIED IMMUNOGENICITY
Abstract
The present invention relates to a method of selecting a protein
variant having modified immunogenicity as compared to the parent
protein comprising the steps obtaining antibody binding peptide
sequences, using the sequences to localise epitope sequences on the
3-dimensional structure of parent protein, defining an epitope area
including amino acids situated within 5 .ANG. from the epitope
amino acids constituting the epitope sequence, changing one or more
of the amino acids defining the epitope area of the parent protein
by genetical engineering mutations of a DNA sequence encoding the
parent protein, introducing the mutated DNA sequence into a
suitable host, culturing said host and expressing the protein
variant, and evaluating the immunogenicity of the protein variant
using the parent protein as reference. The invention further
relates to the protein variant and use thereof, as well as to a
method for producing said protein variant.
Inventors: |
Roggen; Erwin Ludo; (Lyngby,
DK) ; Ernst; Steffen; (Bronshoj, DK) ;
Svendsen; Allan; (Horsholm, DK) ; Friis; Esben
Peter; (Valby, DK) ; Von Der Osten; Claus;
(Lyngby, DK) |
Correspondence
Address: |
NOVOZYMES NORTH AMERICA, INC.
500 FIFTH AVENUE, SUITE 1600
NEW YORK
NY
10110
US
|
Assignee: |
NOVOZYMES A/S
BAGSVAERD
DK
|
Family ID: |
26068820 |
Appl. No.: |
12/699979 |
Filed: |
February 4, 2010 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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09957806 |
Sep 21, 2001 |
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12699979 |
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PCT/DK01/00293 |
Apr 30, 2001 |
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09957806 |
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60203345 |
May 10, 2000 |
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60277817 |
Mar 21, 2001 |
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Current U.S.
Class: |
435/222 ;
435/212; 435/254.11; 435/320.1; 435/325; 435/348; 435/419 |
Current CPC
Class: |
C07K 5/1021 20130101;
C07K 5/1008 20130101; C07K 5/1019 20130101; C07K 5/1013 20130101;
C12Y 110/03002 20130101; C12N 9/20 20130101; A21D 2/267 20130101;
C12Y 304/21062 20130101; A21D 8/042 20130101; C07K 16/40 20130101;
C11D 3/38645 20130101; C07K 16/4283 20130101; Y10S 435/911
20130101; C12N 9/0061 20130101; C12N 9/54 20130101; A61K 39/00
20130101; C07K 5/101 20130101; C07K 1/047 20130101; C07K 14/001
20130101; C11D 3/38627 20130101 |
Class at
Publication: |
435/222 ;
435/212; 435/320.1; 435/325; 435/348; 435/419; 435/254.11 |
International
Class: |
C12N 9/56 20060101
C12N009/56; C12N 9/48 20060101 C12N009/48; C12N 15/63 20060101
C12N015/63; C12N 5/10 20060101 C12N005/10; C12N 1/15 20060101
C12N001/15 |
Foreign Application Data
Date |
Code |
Application Number |
Apr 28, 2000 |
DK |
PA 2000 00707 |
Feb 28, 2001 |
DK |
PA 2001 00327 |
Claims
1-134. (canceled)
135. A protease variant having modified immunogenicity as compared
to a parent protease, obtainable by a method comprising the steps
of: (a) obtaining antibody binding peptide sequences, (b) using the
sequences to localise epitope sequences on the 3-dimensional
structure of the parent protein, (c) defining an epitope area
including amino acids situated within 5 .ANG. from the epitope
amino acids constituting the epitope sequence, (d) changing one or
more of the amino acids defining the epitope area of the parent
protein by genetic engineering mutations of a DNA sequence encoding
the parent protein, (e) introducing the mutated DNA sequence into a
suitable host, culturing said host and expressing the protein
variant, and (f) evaluating the immunogenicity of the protein
variant using the parent protein as reference.
136. The protease variant of claim 135, wherein the protease is a
subtilisin comprising one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position -6
to A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position -5 to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion; Position -4 to
A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position -2 to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion; Position 3a to
A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position 28a to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion; Position 44a to
A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position 44b to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion; Position 139 to
A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position 148 to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion; Position 149 to
A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion; Position 264a to A, C, D, E, F, G, H, I, K, L,
M, N, P, Q, R, S, T, V, W, Y, insertion, deletion.
137. The protease variant of claim 135, wherein the protease is a
subtilisin comprising one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position -1
to G, V, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, H; Position 1
to V, L, I, W, M, F, Y, S, T, R; Position 2 to G, V, I, M, F, N, Q,
Y, S, T, H; Position 3 to W, M, F, N, Q, Y, S, D, E, R, H; Position
4 to V, L, W, M, F, Y, R; Position 5 to V, L, I, W, M, F, N, Q, Y,
T, R, H; Position 6 to G, V, L, I, W, P, M, N, Q, T, D, E, R, H;
Position 9 to G, V, L, I, W, P, M, F, Q, Y, S, T, R, H; Position 10
to G, A, V, I, W, P, M, N, Q, Y, S, T, D, E, R; Position 12 to G,
A, V, L, I, W, M, F, N, Q, Y, S, T, D, E; Position 14 to V, L, I,
W, P, M, F, N, Q, Y, T, R, H; Position 15 to G, A, V, L, I, W, P,
M, F, N, Q, Y, S, T, E, H; Position 17 to G, A, V, I, W, P, M, F,
Y, H; Position 18 to G, A, L, I, W, P, M, F, N, Q, Y, T, D, E, H;
Position 19 to A, V, I, W, M, F, N, Y, S, T, D, R, H; Position 20
to G, V, L, I, W, M, F, N, Q, Y, S, T, D, E; Position 21 to G, V,
I, W, N, Q, Y, S, T, D, E, R, H; Position 22 to G, V, L, I, W, M,
F, Y, S, T; Position 24 to G, V, L, I, W, M, F, N, Q, Y, S, D, E,
R; Position 25 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R,
H; Position 27 to G, L, I, W, P, M, F, Y, T, H; Position 38 to V,
L, I, W, M, F, N, Q, Y, T, H; Position 39 to G, A, V, L, I, W, M,
F, N, Q, Y, T, D, E, R, H; Position 40 to V, L, I, W, M, F, N, Q,
Y, T, R, H; Position 42 to G, A, L, W, C, M, F, N, Q, Y, S, T, D,
E, R, H; Position 43 to G, L, H; Position 44 to G, V, L, I, W, P,
M, F, Y, S, T; Position 45 to G, V, L, I, W, P, M, F, N, Q, Y, S,
T, D, E, R, H; Position 46 to G, A, L, I, W, P, M, F, Y, H;
Position 47 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R,
H; Position 48 to A, L, I, P, M, F, N, Y, D, H; Position 49 to G,
A, V, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H; Position 50 to G,
A, W, M, N, Q, Y, S, T, D, E, H; Position 51 to V, L, I, W, M, F,
N, Y, R; Position 52 to V, L, I, W, M, F, Y, S, T, R; Position 53
to A, V, L, I, W, M, F, N, Q, Y, S, D, E, H; Position 54 to V, L,
I, W, M, F, S, R; Position 55 to G, A, V, L, I, W, C, M, F, N, Q,
Y, T, D, E, R, K, H; Position 56 to G, V, L, I, W, M, F, N, Q, Y,
S, T, H; Position 57 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D,
E, R, K, H; Position 58 to L, W, M, F, N, Y, R; Position 59 to A,
V, L, I, C, T, H; Position 61 to V, L, I, W, M, F, Y; Position 62
to G, A, L, W, M, F, N, Y, R; Position 64 to G, V, L, I, W, P, C,
M, F, N, Q, Y, S, T, D, E, R, K, H; Position 75 to L; Position 79
to I; Position 80 to G; Position 87 to A, V, L, I, W, M, F, Q, Y,
S, T, D, E, H; Position 89 to G, V, L, I, W, P, F, N, Y, T, E;
Position 91 to G, A, V, L, I, W, P, M, N, Y, S, T, D, E, R, H;
Position 98 to A; Position 99 to V, L, I, W, M, F, Q, Y, H;
Position 100 to G, V, L, I, W, M, F, Y, R, H; Position 101 to V, I,
W, M, F, N, Q, Y, H; Position 102 to V, L, I, W, M, F, Y, R, H, G;
Position 108 to I; Position 109 to N, Position 112 to E; Position
113 to W; Position 115 to I; Position 117 to N; Position 118 to N;
Position 126 to L; Position 127 to G, A, V, I, W, M, F, Y, R, H, L;
Position 128 to I, W; Position 129 to W; Position 130 to W, F, Y,
R; Position 131 to W, Y, R; Position 132 to L, W, M, F, Y, S, H;
Position 133 to A, L, I, W, M, F, Y, R; Position 134 to L, I, W, F,
N, Q, Y, R, H; Position 136 to G, A, W, P, N, Y, S, T, D, E, H;
Position 137 to G, A, V, I, W, P, M, N, Y, H; Position 140 to G, A,
V, L, I, W, P, M, F, N, Q, Y, S, T, H; Position 141 to G, V, L, I,
W, P, M, F, Q, S, D, E, H; Position 143 to V, L, I, P, M, F, N, Y,
R; Position 144 to L, W, P, M, F, N, Q, Y, S, D, E, R, H; Position
145 to G, V, L, I, W, M, F, Q, Y, D, E, R, H; Position 146 to G, A,
W, L, I, W, M, F, N, Q, Y, T, D, E, R, H; Position 155 to V, L, I,
W, M, F, Y, R; Position 156 to V, I, W, F, R; Position 157 to G, A,
V, L, I, W, M, F, Y, T, R, H; Position 158 to V, L, I, W, M, F, Y;
Position 159 to A, W, M, Y, T, R, H; Position 160 to W, M, F, Y, R,
H; Position 161 to I, W, M, F, Y, H; Position 167 to R, K; Position
171 to D; Position 172 to G, A, V, L, I, S, T, H; Position 173 to
G, A, V, L, I, W, M, F, N, Q, Y, S, T, E, H; Position 181 to G, A,
V, L, I, W, C, M, F, Q, Y, T, D, R, K, H; Position 182 to A, V, L,
I, W, C, M, F, N, Q, Y, S, T, D, E, H; Position 183 to G, A, V, L,
W, C, M, F, N, Q, Y, S, T, E, R, H; Position 184 to A, V, L, I, W,
C, M, F, N, Q, Y, T, E, H; Position 185 to G, A, V, L, I, W, C, M,
F, N, Q, Y, T, E, H; Position 186 to G, A, V, L, W, M, F, N, Q, Y,
S, T, D, E, R, H; Position 188 to G, A, V, L, W, F, S, R, K;
Position 189 to W, F; Position 191 to A, V, L, I, W, M, F, Y, T, R,
H; Position 192 to G, L, I, W, M, N, Q, Y, S, T, D, R, H; Position
194 to W, N, Q, Y, D, H; Position 195 to W, P, Y; Position 196 to
G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H; Position 203
to V, F, Y, R, H; Position 204 to I, W, M, Y, H; Position 206 to F;
Position 209 to Y, R; Position 210 to W, F, Y; Position 211 to L,
W, M, F, Y, H; Position 212 to V, L, I, W, M, F, Y, T, R, H;
Position 214 to W, Y, R; Position 215 to A, L, I, W, M, F, Y;
Position 216 to A, L, I, W, M, F, Y, R; Position 217 to W, R;
Position 218 to G, A, L, W, P, M, F, Y, R, H; Position 221 to S;
Position 236 to S; Position 240 to N; Position 241 to W; Position
243 to N; Position 245 to Q; Position 247 to G, V, I, W, P, F, Y,
S, T, R; Position 248 to W, P, F, Y, E, R, H; Position 249 to L, W,
P, F, S, D, E, H; Position 251 to G, L, I, W, P, M, F, Y, H;
Position 252 to G, A, W, P, N, Q, Y, T, E, R, H; Position 254 to G,
V, L, I, W, M, F, N, Q, Y, S, D, E, R, H; Position 255 to G, L, W,
M, F, N, Y, T, D, H; Position 256 to G, A, V, L, I, W, M, F, Q, Y,
S, T, D, H; Position 257 to G, A, L, I, W, C, M, F, N, Q, Y, S, T,
D, E, K, H; Position 258 to G, A, V, L, I, W, C, M, F, N, Q, Y, S,
T, E, K, H; Position 259 to A, V, I, W, M, F, N, Q, Y, S, T, E, R;
Position 260 to L, I, W, M, F, Y, T, H; Position 261 to L, N, S, H;
Position 262 to G, A, V, L, I, W, P, F, N, Q, Y, T, D, E, R, H;
Position 263 to G, A, V, L, I, P, C, M, N, Q, Y, S, T, R, K;
Position 265 to V, L, I, W, M, F, Y; Position 269 to G, A, V, L, I,
W, M, F, N, Q, Y, S, T, E, R, H; Position 271 to A, L, I, W, P, M,
F, N, Y, S, T, R, H; Position 272 to G, A, V, L, I, W, P, M, F, N,
Q, Y, T, D, E, H; Position 275 to G, A, V, L, I, W, M, F, N, Y, T,
D.
138. The protease variant of claim 135, wherein the protease is a
subtilisin comprising one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position -1
to Deletion; Position 9 to Insertion, deletion; Position 10 to
Insertion, deletion; Position 12 to Insertion, deletion; Position
14 to Insertion, deletion; Position 15 to Insertion, deletion;
Position 17 to Insertion, deletion; Position 18 to Insertion,
deletion; Position 19 to Insertion, deletion; Position 20 to
Insertion, deletion; Position 21 to Insertion, deletion; Position
22 to Insertion, deletion; Position 24 to Insertion, deletion;
Position 25 to Insertion, deletion; Position 46 to Insertion,
deletion; Position 47 to Insertion, deletion; Position 48 to
Insertion, deletion; Position 49 to Insertion, deletion; Position
50 to Insertion, deletion; Position 51 to Insertion, deletion;
Position 52 to Insertion, deletion; Position 53 to Insertion,
deletion; Position 54 to Insertion, deletion; Position 55 to
Insertion, deletion; Position 58 to Insertion, deletion; Position
59 to Insertion, deletion; Position 61 to Insertion, deletion;
Position 64 to Insertion, deletion; Position 78 to Insertion:
Position 80 to Insertion; Position 91 to Insertion, deletion;
Position 98 to Deletion; Position 99 to Deletion; Position 102 to
Deletion; Position 105 to Insertion; Position 108 to Insertion;
Position 109 to Insertion; Position 112 to Insertion; Position 113
to Insertion; Position 115 to Insertion; Position 116 to Insertion;
Position 117 to Insertion; Position 118 to Insertion; Position 131
to Deletion; Position 134 to Insertion, deletion; Position 136 to
Insertion, deletion; Position 137 to Insertion, deletion; Position
140 to Insertion, deletion; Position 141 to Insertion, deletion;
Position 143 to Insertion, deletion; Position 144 to Insertion,
deletion; Position 145 to Insertion, deletion; Position 146 to
Insertion, deletion; Position 171 to Deletion; Position 172 to
Deletion; Position 173 to Deletion; Position 181 to Deletion;
Position 182 to Deletion; Position 183 to Deletion; Position 184 to
Deletion; Position 185 to Deletion; Position 186 to Deletion;
Position 188 to Deletion; Position 189 to Deletion; Position 191 to
Deletion; Position 192 to Deletion; Position 195 to Deletion;
Position 196 to Insertion, deletion; Position 221 to Insertion;
Position 236 to Insertion; Position 237 to Insertion; Position 238
to Insertion; Position 239 to Insertion; Position 240 to Insertion;
Position 241 to Insertion; Position 242 to Insertion; Position 243
to Insertion; Position 244 to Insertion; Position 245 to Insertion;
Position 247 to Insertion, deletion; Position 248 to Insertion,
deletion; Position 249 to Insertion, deletion; Position 251 to
Insertion, deletion; Position 252 to Insertion, deletion; Position
254 to Insertion, deletion; Position 255 to Insertion, deletion;
Position 256 to Insertion, deletion; Position 257 to Insertion,
deletion; Position 258 to Insertion, deletion; Position 259 to
Insertion, deletion; Position 260 to Insertion, deletion; Position
261 to Insertion, deletion; Position 262 to Insertion, deletion;
Position 263 to Insertion, deletion; Position 265 to Insertion,
deletion; Position 269 to Insertion, deletion; Position 271 to
Insertion, deletion; Position 272 to Insertion, deletion; Position
275 to Insertion, deletion.
139. The protease variant of claim 135, wherein the protease is a
subtilisin comprising one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position 7
to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H; Position 8
to G, A, L, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H; Position
13 to G, L, I, W, P, M, F, N, Q, Y, S, D, E, H; Position 16 to G,
A, V, L, I, W, P, M, F, N, Q, Y, S, D, E, R, H; Position 23 to G,
A, V, L, I, W, M, F, Y, E, R, H; Position 26 to G, A, V, L, I, W,
M, F, N, Q, Y, S, T, D, E, R, H; Position 28 to G, A, V, L, I, W,
P, M, F, N, Q, Y, S, T, D, E, R, K, H; Position 29 to G, A, V, L,
I, W, P, M, F, N, Q, Y, S, T, D, E, R, K, H; Position 33 to V, L,
I, W, C, M, F, N, Q, Y, R, H; Position 35 to G, A, V, L, I, W, M,
F, N, Q, Y, S, T, D, E, R, K, H; Position 36 to V, L, I, W, P, M,
F, N, Y, S, T, R, H; Position 37 to L, I, W, M, F, N, Q, Y, S, R,
H; Position 41 to G, V, L, I, W, M, F, N, Q, Y, S, T, R, H;
Position 60 to G, A, V, L, I, W, C, M, F, Q, Y, T, D, R, K, H;
Position 63 to G, A, V, L, I, W, M, F, Y, T, R, H; Position 73 to
A; Position 74 to A; Position 81 to V; Position 82 to L; Position
86 to G, A, V, L, I, W, M, F, N, Q, Y, T, D, E, R, H; Position 88
to A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, H; Position 92 to
G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, K, H; Position
93 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, K, H;
Position 94 to G, V, L, I, W, P, M, F, N, Y, T, D, E, K, H;
Position 96 to L, W, F, Y, R, K; Position 97 to V, L, W, C, M, F,
Y, H; Position 111 to I; Position 114 to A; Position 119 to M;
Position 124 to M; Position 135 to G, L, P, C, N, Q, T, R, H;
Position 138 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R,
H; Position 142 to G, A, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E,
R, K, H; Position 147 to G, A, V, L, W, M, F, N, Q, Y, S, T, D, E,
R, K, H; Position 151 to G, V, L, I, W, P, C, M, F, N, Q, Y, S, T,
D, E, R, K, H; Position 162 to I, W, F, Y, R; Position 163 to V, W,
M, F, H; Position 168 to G, V, L, I, W, C, M, F, N, Q, Y, S, T, D,
E, R, K, H; Position 169 to C, E, F, G, H, I, K, L, M, N, Q, R, T,
V, W, Y; Position 174 to G, A, L, I, W, P, C, M, F, N, Q, Y, S, T,
D, E, R, K, H; Position 176 to G, A, V, L, I, W, P, C, M, F, N, Q,
Y, S, T, D, E, R, K, H; Position 179 to G, A, V, L, I, W, P, M, F,
N, Q, Y, S, T, D, E, R, K, H; Position 187 to A, V, L, I, W, M, F,
Y, R; Position 190 to G, A, V, L, I, W, C, M, F, N, Q, Y, S, T, R,
K, H; Position 193 to G, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R,
H; Position 197 to G, V, L, I, W, P, M, F, Q, Y, S, T, H; Position
198 to G, A, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H;
Position 205 to W, F, Y, R, K; Position 208 to A, V, L, I, W, C, M,
F, Y, T, R, K, H; Position 219 to G, A, V, L, I, W, F, Y, R, H;
Position 222 to M; Position 232 to A; Position 233 to L; Position
234 to I; Position 250 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T,
D, E, R, H; Position 267 to G, A, V, L, I, W, M, F, N, Q, Y, S, T,
D, E, R, H; Position 268 to G, V, L, I, W, C, M, N, Q, Y, S, T, D,
E, R, K, H; Position 270 to G, L, I, W, P, M, F, N, Q, Y, S, T, D,
E, R, K, H; Position 273 to G, A, V, L, I, W, P, M, F, N, Q, Y, S,
T, D, E, R, K, H; Position 274 to W, P, M, F, N, Q, Y, T, D, E, R,
H.
140. The protease variant of claim 135, wherein the protease is a
subtilisin comprising one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position 13
to Insertion, deletion; Position 16 to Insertion, deletion;
Position 23 to Insertion, deletion; Position 26 to Insertion,
deletion; Position 28 to Insertion, deletion; Position 29 to
Insertion, deletion; Position 35 to Deletion; Position 60 to
Insertion, deletion; Position 63 to Insertion; Position 81 to
Insertion; Position 82 to Insertion; Position 92 to Insertion,
deletion; Position 93 to Insertion, deletion; Position 94 to
Insertion, deletion; Position 96 to Deletion, Position 106 to
Insertion, Position 111 to Insertion, Position 114 to Insertion,
Position 119 to Insertion, Position 124 to Insertion, Position 138
to Insertion, deletion; Position 142 to Insertion, deletion;
Position 147 to Insertion, deletion; Position 151 to Insertion,
deletion; Position 174 to Insertion, deletion; Position 176 to
Insertion, deletion; Position 179 to Insertion, deletion; Position
187 to Deletion; Position 190 to Deletion; Position 193 to
Deletion; Position 197 to Insertion, deletion; Position 198 to
Insertion, deletion; Position 232 to Insertion, Position 233 to
Insertion, Position 234 to Insertion, Position 246 to Insertion,
Position 250 to Insertion, deletion; Position 267 to Insertion,
deletion; Position 268 to Insertion, deletion; Position 270 to
Insertion, deletion; Position 273 to Insertion, deletion.
141. The protease variant of claim 135, wherein the protease is a
savinase-like subtilisin comprising one or more of the following
substitutions corresponding to any of the following in SEQ ID NO:
10: Position 2 to G, V, I, M, F, N, Q, Y, S, T, H, Position 3 to W,
M, F, N, Q, Y, S, D, E, R, H, Position 4 to V, L, W, M, F, Y, R,
Position 6 to G, V, L, I, W, P, M, N, Q, T, D, E, R, H, Position 9
to G, V, L, I, W, P, M, F, Q, Y, S, T, R, H, insertion, deletion,
Position 10 to G, A, V, I, W, P, M, N, Q, Y, S, T, D, E, R,
insertion, deletion, Position 12 to G, A, V, L, I, W, M, F, N, Q,
Y, S, T, D, E, insertion, deletion, Position 14 to V, L, I, W, P,
M, F, N, Q, Y, T, R, H, insertion, deletion, Position 15 to G, A,
V, L, I, W, P, M, F, N, Q, Y, S, T, E, H, insertion, deletion,
Position 17 to G, A, V, I, W, P, M, F, Y, H, insertion, deletion,
Position 18 to G, A, L, I, W, P, M, F, N, Q, Y, T, D, E, H,
insertion, deletion, Position 19 to A, V, I, W, M, F, N, Y, S, T,
D, R, H, insertion, deletion, Position 20 to G, V, L, I, W, M, F,
N, Q, Y, S, T, D, E, insertion, deletion, Position 21 to G, V, I,
W, N, Q, Y, S, T, D, E, R, H, insertion, deletion, Position 22 to
G, V, L, I, W, M, F, Y, S, T, insertion, deletion, Position 24 to
G, V, L, I, W, M, F, N, Q, Y, S, D, E, R, insertion, deletion,
Position 25 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 27 to G, L, I, W, P, M, F, Y, T, H,
Position 37 to L, I, W, M, F, N, Q, Y, S, R, H, Position 40 to V,
L, I, W, M, F, N, Q, Y, T, R, H, Position 42 to G, A, L, W, C, M,
F, N, Q, Y, S, T, D, E, R, H, Position 43 to G, L, H, Position 44
to G, V, L, I, W, P, M, F, Y, S, T, Position 45 to G, V, L, I, W,
P, M, F, N, Q, Y, S, T, D, E, R, H, Position 46 to G, A, L, I, W,
P, M, F, Y, H, insertion, deletion, Position 47 to G, A, V, L, I,
W, P, M, F, N, Q, Y, S, T, D, E, R, H, insertion, deletion,
Position 48 to A, L, I, P, M, F, N, Y, D, H, insertion, deletion,
Position 50 to G, A, W, M, N, Q, Y, S, T, D, E, H, insertion,
deletion, Position 51 to V, L, I, W, M, F, N, Y, R, deletion,
insertion, Position 54 to V, L, I, W, M, F, S, R, deletion,
insertion, Position 55 to G, A, V, L, I, W, C, M, F, N, Q, Y, T, D,
E, R, K, H, deletion, insertion, Position 57 to G, A, V, L, I, W,
M, F, N, Q, Y, S, T, D, E, R, K, H, Position 58 to L, W, M, F, N,
Y, R, insertion, deletion, Position 59 to A, V, L, I, C, T, H,
insertion, deletion, Position 61 to V, L, I, W, M, F, Y, insertion,
deletion, Position 64 to G, V, L, I, W, P, C, M, F, N, Q, Y, S, T,
D, E, R, K, H, insertion, deletion, Position 75 to L, Position 78
to insertion, Position 79 to I, Position 87 to A, V, L, I, W, M, F,
Q, Y, S, T, D, E, H, Position 89 to G, V, L, I, W, P, F, N, Y, T,
E, Position 91 to G, A, V, L, I, W, P, M, N, Y, S, T, D, E, R, H,
insertion, deletion, Position 98 to A, deletion, Position 100 to G,
V, L, I, W, M, F, Y, R, H, Position 101 to V, I, W, M, F, N, Q, Y,
H, Position 102 to V, L, I, W, M, F, Y, R, H, G, deletion, Position
109 to N, insertion, Position 112 to E, insertion, Position 113 to
W, insertion, Position 116 to insertion, Position 117 to N,
insertion, Position 126 to L, Position 127 to G, A, V, I, W, M, F,
Y, R, H, L, Position 128 to I, W, Position 129 to W, Position 130
to W, F, Y, R, Position 131 to W, Y, R, deletion, Position 132 to
L, W, M, F, Y, S, H, Position 133 to A, L, I, W, M, F, Y, R,
Position 134 to L, I, W, F, N, Q, Y, R, H, insertion, deletion,
Position 136 to G, A, W, P, N, Y, S, T, D, E, H, insertion,
deletion, Position 137 to G, A, V, I, W, P, M, N, Y, H, insertion,
deletion, Position 140 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T,
H, insertion, deletion, Position 141 to G, V, L, I, W, P, M, F, Q,
S, D, E, H, insertion, deletion, Position 143 to V, L, I, P, M, F,
N, Y, R, insertion, deletion, Position 144 to L, W, P, M, F, N, Q,
Y, S, D, E, R, H, insertion, deletion, Position 145 to G, V, L, I,
W, M, F, Q, Y, D, E, R, H, insertion, deletion, Position 146 to G,
A, W, L, I, W, M, F, N, Q, Y, T, D, E, R, H, insertion, deletion,
Position 155 to V, L, I, W, M, F, Y, R, Position 156 to V, I, W, F,
R, Position 157 to G, A, V, L, I, W, M, F, Y, T, R, H, Position 158
to V, L, I, W, M, F, Y, Position 160 to W, M, F, Y, R, H, Position
161 to I, W, M, F, Y, H, Position 167 to R, K, Position 170 to A,
C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; Position
171 to D, deletion, Position 172 to G, A, V, L, I, S, T, H,
deletion, Position 173 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, E,
H, deletion, Position 181 to G, A, V, L, I, W, C, M, F, Q, Y, T, D,
R, K, H, deletion, Position 183 to G, A, V, L, W, C, M, F, N, Q, Y,
S, T, E, R, H, deletion, Position 184 to A, V, L, I, W, C, M, F, N,
Q, Y, T, E, H, deletion, Position 185 to G, A, V, L, I, W, C, M, F,
N, Q, Y, T, E, H, deletion, Position 186 to G, A, V, L, W, M, F, N,
Q, Y, S, T, D, E, R, H, deletion, Position 188 to G, A, V, L, W, F,
S, R, K, deletion, Position 189 to W, F, deletion, Position 191 to
A, V, L, I, W, M, F, Y, T, R, H, deletion, Position 192 to G, L, I,
W, M, N, Q, Y, S, T, D, R, H, deletion, Position 194 to W, N, Q, Y,
D, H, Position 195 to W, P, Y, deletion, Position 197 to G, V, L,
I, W, P, M, F, Q, Y, S, T, H, insertion, deletion, Position 203 to
V, F, Y, R, H, Position 206 to F, Position 209 to Y, R, Position
210 to W, F, Y, Position 212 to V, L, I, W, M, F, Y, T, R, H,
Position 214 to W, Y, R, Position 216 to A, L, I, W, M, F, Y, R,
Position 217 to W, R, Position 218 to G, A, L, W, P, M, F, Y, R, H,
Position 221 to S, insertion, Position 236 to S, insertion,
Position 237 to insertion, Position 239 to insertion, Position 240
to N, insertion, Position 241 to W, insertion, Position 242 to
insertion, Position 244 to insertion, Position 245 to Q, insertion,
Position 247 to G, V, I, W, P, F, Y, S, T, R, insertion, deletion,
Position 248 to W, P, F, Y, E, R, H, insertion, deletion, Position
251 to G, L, I, W, P, M, F, Y, H, insertion, deletion, Position 252
to G, A, W, P, N, Q, Y, T, E, R, H, insertion, deletion, Position
255 to G, L, W, M, F, N, Y, T, D, H, insertion, deletion, Position
256 to G, A, V, L, I, W, M, F, Q, Y, S, T, D, H, insertion,
deletion, Position 257 to G, A, L, I, W, C, M, F, N, Q, Y, S, T, D,
E, K, H, insertion, deletion, Position 258 to G, A, V, L, I, W, C,
M, F, N, Q, Y, S, T, E, K, H, insertion, deletion, Position 259 to
A, V, I, W, M, F, N, Q, Y, S, T, E, R, insertion, deletion,
Position 260 to L, I, W, M, F, Y, T, H, insertion, deletion,
Position 261 to L, N, S, H, insertion, deletion, Position 262 to G,
A, V, L, I, W, P, F, N, Q, Y, T, D, E, R, H, insertion, deletion,
Position 263 to G, A, V, L, I, P, C, M, N, Q, Y, S, T, R, K,
insertion, deletion, Position 265 to V, L, I, W, M, F, Y,
insertion, deletion, Position 271 to A, L, I, W, P, M, F, N, Y, S,
T, R, H, insertion, deletion, Position 272 to G, A, V, L, I, W, P,
M, F, N, Q, Y, T, D, E, H, insertion, deletion, Position 275 to G,
A, V, L, I, W, M, F, N, Y, T, D, insertion, deletion.
142. The protease variant of claim 141, wherein the savinase-like
subtilisin comprises one or more of the following substitutions
corresponding to any of the following in SEQ ID NO: 10: Position 6
to G, V, L, I, W, P, M, N, Q, T, D, E, R, H, Position 9 to G, V, L,
I, W, P, M, F, Q, Y, S, T, R, H, insertion, deletion, Position 10
to G, A, V, I, W, P, M, N, Q, Y, S, T, D, E, R, insertion,
deletion, Position 14 to V, L, I, W, P, M, F, N, Q, Y, T, R, H,
insertion, deletion, Position 15 to G, A, V, L, I, W, P, M, F, N,
Q, Y, S, T, E, H, insertion, deletion, Position 17 to G, A, V, I,
W, P, M, F, Y, H, insertion, deletion, Position 18 to G, A, L, I,
W, P, M, F, N, Q, Y, T, D, E, H, insertion, deletion, Position 19
to A, V, I, W, M, F, N, Y, S, T, D, R, H, insertion, deletion,
Position 20 to G, V, L, I, W, M, F, N, Q, Y, S, T, D, E, insertion,
deletion, Position 21 to G, V, I, W, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 37 to L, I, W, M, F, N, Q, Y, S, R,
H, Position 43 to G, L, H, Position 45 to G, V, L, I, W, P, M, F,
N, Q, Y, S, T, D, E, R, H, Position 47 to G, A, V, L, I, W, P, M,
F, N, Q, Y, S, T, D, E, R, H, insertion, deletion, Position 50 to
G, A, W, M, N, Q, Y, S, T, D, E, H, insertion, deletion, Position
51 to V, L, I, W, M, F, N, Y, R, deletion, insertion, Position 54
to V, L, I, W, M, F, S, R, deletion, insertion, Position 59 to A,
V, L, I, C, T, H, insertion, deletion, Position 89 to G, V, L, I,
W, P, F, N, Y, T, E, Position 91 to G, A, V, L, I, W, P, M, N, Y,
S, T, D, E, R, H, insertion, deletion, Position 101 to V, I, W, M,
F, N, Q, Y, H, Position 109 to N, insertion, Position 112 to E,
insertion, Position 113 to W, insertion, Position 127 to G, A, V,
I, W, M, F, Y, R, H, L, Position 128 to I, W, Position 129 to W,
Position 130 to W, F, Y, R, Position 131 to W, Y, R, deletion,
Position 133 to A, L, I, W, M, F, Y, R, Position 136 to G, A, W, P,
N, Y, S, T, D, E, H, insertion, deletion, Position 137 to G, A, V,
I, W, P, M, N, Y, H, insertion, deletion, Position 140 to G, A, V,
L, I, W, P, M, F, N, Q, Y, S, T, H, insertion, deletion, Position
141 to G, V, L, I, W, P, M, F, Q, S, D, E, H, insertion, deletion,
Position 143 to V, L, I, P, M, F, N, Y, R, insertion, deletion,
Position 144 to L, W, P, M, F, N, Q, Y, S, D, E, R, H, insertion,
deletion, Position 145 to G, V, L, I, W, M, F, Q, Y, D, E, R, H,
insertion, deletion, Position 146 to G, A, W, L, I, W, M, F, N, Q,
Y, T, D, E, R, H, insertion, deletion, Position 155 to V, L, I, W,
M, F, Y, R, Position 157 to G, A, V, L, I, W, M, F, Y, T, R, H,
Position 158 to V, L, I, W, M, F, Y, Position 160 to W, M, F, Y, R,
H, Position 161 to I, W, M, F, Y, H, Position 167 to R, K, Position
170 to A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y;
Position 171 to D, deletion, Position 172 to G, A, V, L, I, S, T,
H, deletion, Position 173 to G, A, V, L, I, W, M, F, N, Q, Y, S, T,
E, H, deletion, Position 181 to G, A, V, L, I, W, C, M, F, Q, Y, T,
D, R, K, H, deletion, Position 184 to A, V, L, I, W, C, M, F, N, Q,
Y, T, E, H, deletion, Position 185 to G, A, V, L, I, W, C, M, F, N,
Q, Y, T, E, H, deletion, Position 186 to G, A, V, L, W, M, F, N, Q,
Y, S, T, D, E, R, H, deletion, Position 188 to G, A, V, L, W, F, S,
R, K, deletion, Position 189 to W, F, deletion, Position 192 to G,
L, I, W, M, N, Q, Y, S, T, D, R, H, deletion, Position 194 to W, N,
Q, Y, D, H, Position 195 to W, P, Y, deletion, Position 197 to G,
V, L, I, W, P, M, F, Q, Y, S, T, H, insertion, deletion, Position
203 to V, F, Y, R, H, Position 210 to W, F, Y, Position 218 to G,
A, L, W, P, M, F, Y, R, H, Position 236 to S, insertion, Position
237 to insertion, Position 239 to insertion, Position 240 to N,
insertion, Position 241 to W, insertion, Position 242 to insertion,
Position 244 to insertion, Position 245 to Q, insertion, Position
247 to G, V, I, W, P, F, Y, S, T, R, insertion, deletion, Position
251 to G, L, I, W, P, M, F, Y, H, insertion, deletion, Position 255
to G, L, W, M, F, N, Y, T, D, H, insertion, deletion, Position 256
to G, A, V, L, I, W, M, F, Q, Y, S, T, D, H, insertion, deletion,
Position 257 to G, A, L, I, W, C, M, F, N, Q, Y, S, T, D, E, K, H,
insertion, deletion, Position 258 to G, A, V, L, I, W, C, M, F, N,
Q, Y, S, T, E, K, H, insertion, deletion, Position 260 to L, I, W,
M, F, Y, T, H, insertion, deletion, Position 262 to G, A, V, L, I,
W, P, F, N, Q, Y, T, D, E, R, H, insertion, deletion, Position 265
to V, L, I, W, M, F, Y, insertion, deletion, Position 271 to A, L,
I, W, P, M, F, N, Y, S, T, R, H, insertion, deletion, Position 272
to G, A, V, L, I, W, P, M, F, N, Q, Y, T, D, E, H, insertion,
deletion, Position 275 to G, A, V, L, I, W, M, F, N, Y, T, D,
insertion, deletion.
143. The savinase-like subtilisin of claim 141, wherein the
subtilisin has at least 81% homology to SEQ ID NO: 24.
144. The savinase-like subtilisin of claim 141, wherein the
subtilisin has any of the amino acid sequence of SEQ ID NO: 24, 26,
27, 28, 29, 30, 31, 32, 34, 35.
145. The protein variant of claim 135, wherein the protease is a
savinase-like subtilisin comprising one or more of the following
substitutions corresponding to any of the following in SEQ ID NO:
10: Position 8 to G, A, L, W, P, C, M, F, N, Q, Y, S, T, D, E, R,
K, H, Position 16 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, D, E,
R, H, insertion, deletion, Position 23 to G, A, V, L, I, W, M, F,
Y, E, R, H, insertion, deletion, Position 26 to G, A, V, L, I, W,
M, F, N, Q, Y, S, T, D, E, R, H, insertion, deletion, Position 35
to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, K, H, deletion,
Position 38 to V, L, I, W, M, F, N, Q, Y, T, H, Position 39 to G,
A, V, L, I, W, M, F, N, Q, Y, T, D, E, R, H, Position 41 to G, V,
L, I, W, M, F, N, Q, Y, S, T, R, H, Position 60 to G, A, V, L, I,
W, C, M, F, Q, Y, T, D, R, K, H, insertion, deletion, Position 73
to A, Position 74 to A, Position 80 to G, insertion, Position 81 to
V, insertion, Position 86 to G, A, V, L, I, W, M, F, N, Q, Y, T, D,
E, R, H, Position 88 to A, V, L, I, W, M, F, N, Q, Y, S, T, D, E,
R, H, Position 90 to A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R,
S, T, V, W, Y, insertion, deletion, Position 93 to G, A, V, L, I,
W, P, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion, deletion,
Position 108 to I, insertion, Position 111 to I, insertion,
Position 124 to M, insertion, Position 135 to G, L, P, C, N, Q, T,
R, H, Position 142 to G, A, L, I, W, P, C, M, F, N, Q, Y, S, T, D,
E, R, K, H, insertion, deletion, Position 147 to G, A, V, L, W, M,
F, N, Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position 148
to G, A, V, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H,
insertion, deletion, Position 149 to G, A, V, L, I, W, P, C, M, F,
N, Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position 151 to
G, V, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 163 to V, W, M, F, H, Position 168 to G, V, L,
I, W, C, M, F, N, Q, Y, S, T, D, E, R, K, H, Position 169 to C, E,
F, G, H, I, K, L, M, N, Q, R, T, V, W, Y, Position 174 to G, A, L,
I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 179 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T,
D, E, R, K, H, insertion, deletion, Position 190 to G, A, V, L, I,
W, C, M, F, N, Q, Y, S, T, R, K, H, deletion, Position 193 to G, V,
L, I, W, M, F, N, Q, Y, S, T, D, E, R, H, deletion, Position 196 to
G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H, insertion,
deletion, Position 208 to A, V, L, I, W, C, M, F, Y, T, R, K, H,
Position 213 to N, oN, E, Position 215 to A, L, I, W, M, F, Y,
Position 232 to A, insertion, Position 233 to L, insertion,
Position 234 to I, insertion, Position 246 to insertion, Position
250 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 254 to G, V, L, I, W, M, F, N, Q, Y,
S, D, E, R, H, insertion, deletion, Position 267 to G, A, V, L, I,
W, M, F, N, Q, Y, S, T, D, E, R, H, insertion, deletion, Position
268 to G, V, L, I, W, C, M, N, Q, Y, S, T, D, E, R, K, H,
insertion, deletion, Position 269 to G, A, V, L, I, W, M, F, N, Q,
Y, S, T, E, R, H, insertion, deletion, Position 273 to G, A, V, L,
I, W, P, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion.
146. The savinase-like subtilisin of claim 145, wherein the
subtilisin has at least 81% homology to SEQ ID NO: 24.
147. The savinase-like subtilisin of claim 146, wherein the
subtilisin has any of the amino acid sequence of SEQ ID NO: 24, 26,
27, 28, 29, 30, 31, 32, 34, 35.
148. The protease variant of claim 135 having modified
immunogenicity as compared to its parent protein having at least
81% homology to SEQ ID NO: 25 comprising one or more of the
following substitutions corresponding to any of the following in
SEQ ID NO: 25: Position 21 to G, V, I, W, N, Q, Y, S, T, D, E, R,
H, insertion, deletion, Position 27 to G, L, I, W, P, M, F, Y, T,
H, Position 50 to G, A, W, M, N, Q, Y, S, T, D, E, H, insertion,
deletion, Position 52 to V, L, I, W, M, F, Y, S, T, R, deletion,
insertion, Position 55 to G, A, V, L, I, W, C, M, F, N, Q, Y, T, D,
E, R, K, H, deletion, insertion, Position 129 to W, Position 133 to
A, L, I, W, M, F, Y, R, Position 172 to G, A, V, L, I, S, T, H,
deletion, Position 186 to G, A, V, L, W, M, F, N, Q, Y, S, T, D, E,
R, H, deletion, Position 194 to W, N, Q, Y, D, H, Position 195 to
W, P, Y, deletion, Position 197 to G, V, L, I, W, P, M, F, Q, Y, S,
T, H, insertion, deletion, Position 242 to insertion, Position 249
to L, W, P, F, S, D, E, H, insertion, deletion, Position 252 to G,
A, W, P, N, Q, Y, T, E, R, H, insertion, deletion, Position 254 to
G, V, L, I, W, M, F, N, Q, Y, S, D, E, R, H, insertion, deletion,
Position 257 to G, A, L, I, W, C, M, F, N, Q, Y, S, T, D, E, K, H,
insertion, deletion, Position 260 to L, I, W, M, F, Y, T, H,
insertion, deletion, Position 265 to V, L, I, W, M, F, Y,
insertion, deletion, with the proviso that the amino acids of the
parent enzyme are substituted to another amino acid.
149. The protein variant of claim 135 having modified
immunogenicity as compared to its parent protein having at least
81% homology to SEQ ID NO: 10 comprising one or more of the
following substitutions corresponding to any of the following in
SEQ ID NO: 10: Position 4 to V, L, W, M, F, Y, R, Position 38 to V,
L, I, W, M, F, N, Q, Y, T, H, Position 40 to V, L, I, W, M, F, N,
Q, Y, T, R, H, Position 43 to G, L, H, Position 47 to G, A, V, L,
I, W, P, M, F, N, Q, Y, S, T, D, E, R, H, insertion, deletion,
Position 49 to G, A, V, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 54 to V, L, I, W, M, F, S, R,
deletion, insertion, Position 96 to L, W, F, Y, R, K, deletion,
Position 99 to V, L, I, W, M, F, Q, Y, H, deletion, Position 113 to
W, insertion, Position 131 to W, Y, R, deletion, Position 133 to A,
L, I, W, M, F, Y, R, Position 137 to G, A, V, I, W, P, M, N, Y, H,
insertion, deletion, Position 141 to G, V, L, I, W, P, M, F, Q, S,
D, E, H, insertion, deletion, Position 144 to L, W, P, M, F, N, Q,
Y, S, D, E, R, H, insertion, deletion, Position 170 to A, C, D, E,
F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; Position 173 to G,
A, V, L, I, W, M, F, N, Q, Y, S, T, E, H, deletion, Position 181 to
G, A, V, L, I, W, C, M, F, Q, Y, T, D, R, K, H, deletion, Position
185 to G, A, V, L, I, W, C, M, F, N, Q, Y, T, E, H, deletion,
Position 186 to G, A, V, L, W, M, F, N, Q, Y, S, T, D, E, R, H,
deletion, Position 188 to G, A, V, L, W, F, S, R, K, deletion,
Position 194 to W, N, Q, Y, D, H, Position 203 to V, F, Y, R, H,
Position 210 to W, F, Y, Position 211 to L, W, M, F, Y, H, Position
257 to G, A, L, I, W, C, M, F, N, Q, Y, S, T, D, E, K, H,
insertion, deletion, Position 261 to L, N, S, H, insertion,
deletion, Position 262 to G, A, V, L, I, W, P, F, N, Q, Y, T, D, E,
R, H, insertion, deletion, Position 265 to V, L, I, W, M, F, Y,
insertion, deletion. with the proviso that the amino acids of the
parent enzyme are substituted to another amino acid.
150. The protein variant of claim 135 having modified
immunogenicity as compared to its parent protein having at least
81% homology to SEQ ID NO: 11 comprising one or more of the
following substitutions corresponding to any of the following in
SEQ ID NO: 11: Position 38 to V, L, I, W, M, F, N, Q, Y, T, H,
Position 40 to V, L, I, W, M, F, N, Q, Y, T, R, H, Position 45 to
G, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H, Position 47 to
G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R, H, insertion,
deletion, Position 49 to G, A, V, I, W, P, M, F, N, Q, Y, S, T, D,
E, R, H, insertion, deletion, Position 50 to G, A, W, M, N, Q, Y,
S, T, D, E, H, insertion, deletion, Position 52 to V, L, I, W, M,
F, Y, S, T, R, deletion, insertion, Position 53 to A, V, L, I, W,
M, F, N, Q, Y, S, D, E, H, deletion, insertion, Position 56 to G,
V, L, I, W, M, F, N, Q, Y, S, T, H, Position 58 to L, W, M, F, N,
Y, R, insertion, deletion, Position 96 to L, W, F, Y, R, K,
deletion, Position 97 to V, L, W, C, M, F, Y, H, Position 98 to A,
deletion, Position 105 to insertion, Position 109 to N, insertion,
Position 113 to W, insertion, Position 115 to I, insertion,
Position 133 to A, L, I, W, M, F, Y, R, Position 136 to G, A, W, P,
N, Y, S, T, D, E, H, insertion, deletion, Position 137 to G, A, V,
I, W, P, M, N, Y, H, insertion, deletion, Position 141 to G, V, L,
I, W, P, M, F, Q, S, D, E, H, insertion, deletion, Position 158 to
V, L, I, W, M, F, Y, Position 159 to A, W, M, Y, T, R, H, Position
172 to G, A, V, L, I, S, T, H, deletion, Position 186 to G, A, V,
L, W, M, F, N, Q, Y, S, T, D, E, R, H, deletion, Position 189 to W,
F, deletion, Position 192 to G, L, I, W, M, N, Q, Y, S, T, D, R, H,
deletion, Position 195 to W, P, Y, deletion, Position 197 to G, V,
L, I, W, P, M, F, Q, Y, S, T, H, insertion, deletion, Position 257
to G, A, L, I, W, C, M, F, N, Q, Y, S, T, D, E, K, H, insertion,
deletion, Position 261 to L, N, S, H, insertion, deletion, Position
265 to V, L, I, W, M, F, Y, insertion, deletion, with the proviso
that the amino acids of the parent enzyme are substituted to
another amino acid.
151. The protein variant of claim 135 having modified
immunogenicity as compared to its parent protein having at least
81% homology to SEQ ID NO: 33 comprising one or more of the
following substitutions corresponding to any of the following in
SEQ ID NO: 33: Position -6 to A, C, D, E, F, G, H, I, K, L, M, N,
P, Q, R, S, T, V, Y, insertion, deletion, Position -5 to A, C, D,
E, F, G, H, I, K, L, M, N, P, Q, R, T, V, W, Y, insertion,
deletion, Position -4 to A, C, D, E, F, G, H, I, K, L, M, N, Q, R,
S, T, V, W, Y, insertion, deletion, Position -2 to A, C, E, F, G,
H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion, deletion,
Position -1 to G, V, L, I, W, C, M, F, N, Q, Y, S, T, D, E, R, H,
deletion, Position 1 to V, L, I, W, M, F, Y, S, T, R, Position 2 to
G, V, I, M, F, N, Q, Y, S, T, H, Position 3a to A, C, D, E, F, G,
H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion, deletion,
Position 5 to V, L, I, W, M, F, N, Q, Y, T, R, H, Position 6 to G,
V, L, I, W, P, M, N, Q, T, D, E, R, H, Position 7 to G, A, V, L, I,
W, P, M, F, N, Q, Y, S, T, D, E, R, H, Position 8 to G, A, L, W, P,
C, M, F, N, Q, Y, S, T, D, E, R, K, H, Position 10 to G, A, V, I,
W, P, M, N, Q, Y, S, T, D, E, R, insertion, deletion, Position 12
to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, insertion,
deletion, Position 13 to G, L, I, W, P, M, F, N, Q, Y, S, D, E, H,
insertion, deletion, Position 14 to V, L, I, W, P, M, F, N, Q, Y,
T, R, H, insertion, deletion, Position 15 to G, A, V, L, I, W, P,
M, F, N, Q, Y, S, T, E, H, insertion, deletion, Position 16 to G,
A, V, L, I, W, P, M, F, N, Q, Y, S, D, E, R, H, insertion,
deletion, Position 17 to G, A, V, I, W, P, M, F, Y, H, insertion,
deletion, Position 18 to G, A, L, I, W, P, M, F, N, Q, Y, T, D, E,
H, insertion, deletion, Position 19 to A, V, I, W, M, F, N, Y, S,
T, D, R, H, insertion, deletion, Position 21 to G, V, I, W, N, Q,
Y, S, T, D, E, R, H, insertion, deletion, Position 22 to G, V, L,
I, W, M, F, Y, S, T, insertion, deletion, Position 23 to G, A, V,
L, I, W, M, F, Y, E, R, H, insertion, deletion, Position 24 to G,
V, L, I, W, M, F, N, Q, Y, S, D, E, R, insertion, deletion,
Position 25 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 26 to G, A, V, L, I, W, M, F, N, Q,
Y, S, T, D, E, R, H, insertion, deletion, Position 27 to G, L, I,
W, P, M, F, Y, T, H, Position 28 to G, A, V, L, I, W, P, M, F, N,
Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position 28a to A,
C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion,
deletion, Position 29 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T,
D, E, R, K, H, insertion, deletion, Position 33 to V, L, I, W, C,
M, F, N, Q, Y, R, H, Position 35 to G, A, V, L, I, W, M, F, N, Q,
Y, S, T, D, E, R, K, H, deletion, Position 37 to L, I, W, M, F, N,
Q, Y, S, R, H, Position 40 to V, L, I, W, M, F, N, Q, Y, T, R, H,
Position 42 to G, A, L, W, C, M, F, N, Q, Y, S, T, D, E, R, H,
Position 43 to G, L, H, Position 44 to G, V, L, I, W, P, M, F, Y,
S, T, Position 44a to A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R,
S, T, V, W, Y, insertion, deletion, Position 44b to A, C, D, E, F,
G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion, deletion,
Position 46 to G, A, L, I, W, P, M, F, Y, H, insertion, deletion,
Position 48 to A, L, I, P, M, F, N, Y, D, H, insertion, deletion,
Position 51 to V, L, I, W, M, F, N, Y, R, deletion, insertion,
Position 52 to V, L, I, W, M, F, Y, S, T, R, deletion, insertion,
Position 53 to A, V, L, I, W, M, F, N, Q, Y, S, D, E, H, deletion,
insertion, Position 55 to G, A, V, L, I, W, C, M, F, N, Q, Y, T, D,
E, R, K, H, deletion, insertion, Position 56 to G, V, L, I, W, M,
F, N, Q, Y, S, T, H, Position 57 to G, A, V, L, I, W, M, F, N, Q,
Y, S, T, D, E, R, K, H, Position 58 to L, W, M, F, N, Y, R,
insertion, deletion, Position 61 to V, L, I, W, M, F, Y, insertion,
deletion, Position 64 to G, V, L, I, W, P, C, M, F, N, Q, Y, S, T,
D, E, R, K, H, insertion, deletion, Position 75 to L, Position 81
to insertion, Position 86 to G, A, V, L, I, W, M, F, N, Q, Y, T, D,
E, R, H, Position 87 to A, V, L, I, W, M, F, Q, Y, S, T, D, E, H,
Position 88 to A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, H,
Position 89 to G, V, L, I, W, P, F, N, Y, T, E, Position 91 to G,
A, V, L, I, W, P, M, N, Y, S, T, D, E, R, H, insertion, deletion,
Position 92 to G, A, V, L, I, W, P, M, F, N, Q, Y, S, T, D, E, R,
K, H, insertion, deletion, Position 94 to G, V, L, I, W, P, M, F,
N, Y, T, D, E, K, H, insertion, deletion, Position 96 to L, W, F,
Y, R, K, deletion, Position 97 to V, L, W, C, M, F, Y, H, Position
98 to deletion, Position 101 to V, I, W, M, F, N, Q, Y, H, Position
102 to V, L, I, W, M, F, Y, R, H, G, deletion, Position 108 to I,
insertion, Position 109 to N, insertion, Position 111 to insertion,
Position 112 to E, insertion, Position 113 to W, insertion,
Position 114 to insertion, Position 115 to I, insertion, Position
117 to N, insertion, Position 118 to N, insertion, Position 119 to
M, insertion, Position 127 to G, A, V, I, W, M, F, Y, R, H, L,
Position 133 to A, L, I, W, M, F, Y, R, Position 134 to L, I, W, F,
N, Q, Y, R, H, insertion, deletion, Position 135 to G, L, P, C, N,
Q, T, R, H, Position 136 to G, A, W, P, N, Y, S, T, D, E, H,
insertion, deletion, Position 137 to G, A, V, I, W, P, M, N, Y, H,
insertion, deletion, Position 138 to G, A, V, L, I, W, P, M, F, N,
Q, Y, S, T, D, E, R, H, insertion, deletion, Position 139 to G, A,
V, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 141 to G, V, L, I, W, P, M, F, Q, S, D, E, H,
insertion, deletion, Position 142 to G, A, L, I, W, P, C, M, F, N,
Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position 144 to L,
W, P, M, F, N, Q, Y, S, D, E, R, H, insertion, deletion, Position
145 to G, V, L, I, W, M, F, Q, Y, D, E, R, H, insertion, deletion,
Position 146 to G, A, W, L, I, W, M, F, N, Q, Y, T, D, E, R, H,
insertion, deletion, Position 147 to G, A, V, L, W, M, F, N, Q, Y,
S, T, D, E, R, K, H, insertion, deletion, Position 148 to G, A, V,
L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 156 to V, I, W, F, R, Position 158 to V, L, I,
W, M, F, Y, Position 160 to W, M, F, Y, R, H, Position 161 to I, W,
M, F, Y, H, Position 162 to I, W, F, Y, R, Position 163 to V, W, M,
F, H, Position 167 to R, K, Position 169 to C, E, F, G, H, I, K, L,
M, N, Q, R, T, V, W, Y, Position 170 to A, C, D, E, F, G, H, I, K,
L, M, N, P, Q, R, S, T, V, W, Y, insertion, deletion, Position 171
to D, deletion, Position 174 to G, A, L, I, W, P, C, M, F, N, Q, Y,
S, T, D, E, R, K, H, insertion, deletion, Position 176 to G, A, V,
L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 182 to A, V, L, I, W, C, M, F, N, Q, Y, S, T, D,
E, H, deletion, Position 186 to G, A, V, L, W, M, F, N, Q, Y, S, T,
D, E, R, H, deletion, Position 188 to G, A, V, L, W, F, S, R, K,
deletion, Position 191 to A, V, L, I, W, M, F, Y, T, R, H,
deletion, Position 192 to G, L, I, W, M, N, Q, Y, S, T, D, R, H,
deletion, Position 193 to G, V, L, I, W, M, F, N, Q, Y, S, T, D, E,
R, H, deletion, Position 194 to W, N, Q, Y, D, H, Position 195 to
W, P, Y, deletion, Position 196 to G, A, V, L, I, W, P, M, F, N, Q,
Y, S, T, D, E, R, H, insertion, deletion, Position 197 to G, V, L,
I, W, P, M, F, Q, Y, S, T, H, insertion, deletion, Position 198 to
G, A, L, I, W, P, C, M, F, N, Q, Y, S, T, D, E, R, K, H, insertion,
deletion, Position 203 to V, F, Y, R, H, Position 205 to W, F, Y,
R, K, Position 215 to A, L, I, W, M, F, Y, Position 216 to A, L, I,
W, M, F, Y, R, Position 217 to W, R, Position 219 to G, A, V, L, I,
W, F, Y, R, H, Position 233 to insertion, Position 234 to I,
insertion, Position 236 to insertion, Position 237 to insertion,
Position 238 to insertion, Position 239 to insertion, Position 240
to insertion, Position 243 to insertion, Position 246 to insertion,
Position 247 to G, V, I, W, P, F, Y, S, T, R, insertion, deletion,
Position 249 to L, W, P, F, S, D, E, H, insertion, deletion,
Position 252 to G, A, W, P, N, Q, Y, T, E, R, H, insertion,
deletion, Position 254 to G, V, L, I, W, M, F, N, Q, Y, S, D, E, R,
H, insertion, deletion, Position 262 to G, A, V, L, I, W, P, F, N,
Q, Y, T, D, E, R, H, insertion, deletion, Position 264a to A, C, D,
E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion,
deletion, Position 270 to G, L, I, W, P, M, F, N, Q, Y, S, T, D, E,
R, K, H, insertion, deletion, Position 273 to G, A, V, L, I, W, P,
M, F, N, Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position
274 to W, P, M, F, N, Q, Y, T, D, E, R, H, Position 275 to G, A, V,
L, I, W, M, F, N, Y, T, D, insertion, deletion, Position 276 to A,
C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, insertion,
deletion, with the proviso that the amino acids of the parent
enzyme are substituted to another amino acid.
152. The protein variant of claim 135 having modified
immunogenicity as compared to its parent protein having at least
81% homology to SEQ ID NO: 33 comprising one or more of the
following substitutions corresponding to any of the following in
SEQ ID NO: 33: Position 5 to V, L, I, W, M, F, N, Q, Y, T, R, H,
Position 22 to G, V, L, I, W, M, F, Y, S, T, insertion, deletion,
Position 26 to G, A, V, L, I, W, M, F, N, Q, Y, S, T, D, E, R, H,
insertion, deletion, Position 28 to G, A, V, L, I, W, P, M, F, N,
Q, Y, S, T, D, E, R, K, H, insertion, deletion, Position 37 to L,
I, W, M, F, N, Q, Y, S, R, H, Position 40 to V, L, I, W, M, F, N,
Q, Y, T, R, H, Position 44 to G, V, L, I, W, P, M, F, Y, S, T,
Position 51 to V, L, I, W, M, F, N, Y, R, deletion, insertion,
Position 52 to V, L, I, W, M, F, Y, S, T, R, deletion, insertion,
Position 55 to G, A, V, L, I, W, C, M, F, N, Q, Y, T, D, E, R, K,
H, deletion, insertion, Position 58 to L, W, M, F, N, Y, R,
insertion, deletion, Position 61 to V, L, I, W, M, F, Y, insertion,
deletion, Position 64 to G, V, L, I, W, P, C, M, F, N, Q, Y, S, T,
D, E, R, K, H, insertion, deletion, Position 87 to A, V, L, I, W,
M, F, Q, Y, S, T, D, E, H, Position 97 to V, L, W, C, M, F, Y, H,
Position 98 to deletion, Position 101 to V, I, W, M, F, N, Q, Y, H,
Position 102 to V, L, I, W, M, F, Y, R, H, G, deletion, Position
109 to N, insertion, Position 112 to E, insertion, Position 118 to
N, insertion, Position 127 to G, A, V, I, W, M, F, Y, R, H, L,
Position 137 to G, A, V, I, W, P, M, N, Y, H, insertion, deletion,
Position 146 to G, A, W, L, I, W, M, F, N, Q, Y, T, D, E, R, H,
insertion, deletion, Position 156 to V, I, W, F, R, Position 158 to
V, L, I, W, M, F, Y, Position 161 to I, W, M, F, Y, H, Position 188
to G, A, V, L, W, F, S, R, K, deletion, Position 192 to G, L, I, W,
M, N, Q, Y, S, T, D, R, H, deletion, Position 194 to W, N, Q, Y, D,
H, Position 195 to W, P, Y, deletion, Position 203 to V, F, Y, R,
H, Position 216 to A, L, I, W, M, F, Y, R, Position 236 to
insertion, Position 237 to insertion, Position 262 to G, A, V, L,
I, W, P, F, N, Q, Y, T, D, E, R, H, insertion, deletion, Position
264a to A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y,
insertion, deletion, with the proviso that the amino acids of the
parent enzyme are substituted to another amino acid.
153. A subtilisin variant comprising one or more of the insertions,
substitutions and/or deletions in any of the positions of claim
136.
154. The variant of claim 153, wherein the subtilisin has at least
80% homology to SEQ ID NO; 10.
155. A composition comprising the protein variant of claim 135.
156. A DNA construct comprising a DNA sequence encoding a protein
variant of claim 135.
157. An expression vector comprising a DNA construct of claim
156.
158. A host cell which is capable of expressing a polypeptide and
comprising a DNA construct of claim 156.
159. A host cell which is capable of expressing a polypeptide and
which is transformed by an expression vector of claim 157.
160. The host cell of claim 159, which is a fungal cell, an insect
cell, a mammalian cell, or a plant cell.
161. A method of producing a protein variant having reduced
immunogenicity as compared to the parent protein, comprising: (a)
culturing the host of claim 159 in a suitable culture medium to
obtain expression and secretion of the protein into the medium,
followed by (b) isolation of the protein from the culture medium.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. application Ser.
No. 09/957,806 filed on Sep. 21, 2001, which is a continuation of
PCT/DK01/00293 filed Apr. 30, 2001 and claims, under 35 U.S.C. 119,
priority or the benefit of Danish application nos. PA 2000 00707
and PA 2001 00327 filed Apr. 28, 2000 and Feb. 28, 2001,
respectively, and U.S. application Nos. 60/203,345 and 60/277,817
filed May 10, 2000 and Mar. 21, 2001, respectively, the contents of
which are fully incorporated herein by reference.
FIELD OF INVENTION
[0002] The present invention relates to a method of selecting a
protein variant having modified immunogenicity as compared to the
parent protein, to the protein variant and use thereof, as well as
to a method for producing said protein variant.
BACKGROUND OF THE INVENTION
[0003] An increasing number of proteins, including enzymes, are
being produced industrially, for use in various industries,
housekeeping and medicine. Being proteins they are likely to
stimulate an immunological response in man and animals, including
an allergic response.
[0004] Depending on the application, individuals get sensitised to
the respective allergens by inhalation, direct contact with skin
and eyes, or injection. The general mechanism behind an allergic
response is divided in a sensitisation phase and a symptomatic
phase. The sensitisation phase involves a first exposure of an
individual to an allergen. This event activates specific T- and
B-lymphocytes, and leads to the production of allergen specific IgE
antibodies (in the present context the antibodies are denoted as
usual, i.e. immunoglobulin E IgE etc.). These IgE antibodies
eventually facilitate allergen capturing and presentation to
T-lymphocytes at the onset of the symptomatic phase. This phase is
initiated by a second exposure to the same or a resembling antigen.
The specific IgE antibodies bind to the specific IgE receptors on
mast cells and basophils, among others, and capture at the same
time the allergen. The polyclonal nature of this process results in
bridging and clustering of the IgE receptors, and subsequently in
the activation of mast cells and basophils. This activation
triggers the release of various chemical mediators involved in the
early as well as late phase reactions of the symptomatic phase of
allergy. Prevention of allergy in susceptible individuals is
therefore a research area of great importance.
[0005] For certain forms of IgE-mediated allergies, a therapy
exists, which comprises repeated administration of allergen
preparations called `allergen vaccines` (Int. Arch. Allergy
Immunol., 1999, vol. 119, pp 1-5). This leads to reduction of the
allergic symptoms, possibly due to a redirection of the immune
response away from the allergic (Th2) pathway and towards the
immunoprotective (Th1) pathway (Int. Arch. Allergy Immunol., 1999,
vol. 119, pp 1-5).
[0006] Various attempts to reduce the immunogenicity of
polypeptides and proteins have been conducted. It has been found
that small changes in an epitope may affect the binding to an
antibody. This may result in a reduced importance of such an
epitope, maybe converting it from a high affinity to a low affinity
epitope, or maybe even result in epitope loss, i.e. that the
epitope cannot sufficiently bind an antibody to elicit an
immunogenic response.
[0007] There is a need for methods to identify epitopes on proteins
and alter these epitopes in order to modify the immunogenicity of
proteins in a targeted manner. Such methods and kits for their
execution can have at least four useful purposes:
[0008] 1) reduce the allergenicity of a commercial protein using
protein engineering.
[0009] 2) reduce the potential of commercial proteins to
cross-react with environmental allergens and hence cause allergic
reactions in people sensitized to the environmental allergens (or
vice versa).
[0010] 3) improve the immunotherapeutic effect of allergen
vaccines.
[0011] 4) assist characterization of clinical allergies in order to
select the appropriate treatment, including allergen
vaccination.
[0012] In WO 99/53038 (Genencor Int.) as well as in prior
references (Kammerer et al, Clin. Exp. Allergy, 1997, vol. 27, pp
1016-1026; Sakakibara et al, J. Vet. Med. Sci., 1998; vol. 60, pp.
599-605), methods are described, which identify linear T-cell
epitopes among a library of known peptide sequences, each
representing part of the primary sequence of the protein of
interest. Further, several similar techniques for localization of
B-cell epitopes are disclosed by Walshet et al, J. Immunol.
Methods, vol. 121, 1275-280, (1989), and by Schoofs et al. J.
Immunol. vol. 140, 611-616, (1987). All of these methods, however,
only leads to identification of linear epitopes, not to
identification of `structural` or `discontinuous` epitopes, which
are found on the 3-dimensional surface of protein molecules and
which comprise amino acids from several discrete sites of the
primary sequence of the protein. For several allergens, it has been
realized that the dominant epitopes are of such discontinuous
nature (Collins et al., Clin. Exp. All. 1996, vol. 26, pp.
36-42).
[0013] Slootstra et al; Molecular Diversity, 2, pp. 156-164, 1996
disclose the screening of a semi-random library of synthetic
peptides for their binding properties to three monoclonal
antibodies by immobilizing the peptides on polyethylene pins and
binding a dilution series of each antibody to the pins. This
reference does not disclose any indication of how the antibody
binding peptide sequences relate to any full protein antigens or
allergens.
[0014] In WO 92/10755 a method for modifying proteins to obtain
less immunogenic variants is described. Randomly constructed
protein variants, revealing a reduced binding of antibodies to the
parent enzyme as compared to the parent enzyme itself, are selected
for the measurement in animal models in terms of allergenicity.
Finally, it is assessed whether reduction in immunogenicity is due
to true elimination of an epitope or a reduction in affinity for
antibodies. This method targets the identification of amino acids
that may be part of structural epitopes by using a complete protein
for assessing antigen binding. The major drawbacks of this approach
are the `trial and error` character, which makes it a lengthy and
expensive process, and the lack of general information on the
epitope patterns. Without this information, the results obtained
for one protein can not be applied on another protein.
[0015] WO 99/47680 (ALK-ABELLO) discloses the identification and
modification of B-cell epitopes by protein engineering. However,
the method is based on crystal structures of Fab-antigen complexes,
and B-cell epitopes are defined as "a section of the surface of the
antigen comprising 15-25 amino acid residues, which are within a
distance from the atoms of the antibody enabling direct
interaction" (p. 3). This publication does not show how one selects
which Fab fragment to use (e.g. to target the most dominant allergy
epitopes) or how one selects the substitutions to be made. Further,
their method cannot be used in the absence of such crystallographic
data for antigen-antibody complexes, which are very cumbersome,
sometimes impossible, to obtain--especially since one would need a
separate crystal structure for each epitope to be changed.
[0016] Hence, it is of interest to establish a general and
efficient method to identify structural epitopes on the
3-dimensional surface of commercial and environmental
allergens.
SUMMARY OF THE INVENTION
[0017] The present invention relates to a method of selecting a
protein variant having modified immunogenicity as compared to a
parent protein, comprising the steps of:
[0018] a) obtaining antibody binding peptide sequences,
[0019] b) using the sequences to localise epitope sequences on the
3-dimensional structure of parent protein,
[0020] c) defining an epitope area including amino acids situated
within 5 .ANG. from the epitope amino acids constituting the
epitope sequence,
[0021] d) changing one or more of the amino acids defining the
epitope area of the parent protein by genetic engineering mutations
of a DNA sequence encoding the parent protein,
[0022] e) introducing the mutated DNA sequence into a suitable
host, culturing said host and expressing the protein variant,
and
[0023] f) evaluating the immunogenicity of the protein variant
using the parent protein as reference.
[0024] A second aspect of the present invention is a protein
variant having modified immunogenicity as compared to its parent
protein. The amino acid sequence of the protein variant differs
from the amino acid sequence of the parent protein with respect to
at least one epitope pattern of the parent protein, such that the
immunogenicity of the protein variant is modified as compared with
the immunogenicity of the parent protein.
[0025] A further aspect of the present invention is a composition
comprising a protein variant as defined above, as well as the use
of the composition for industrial application, such as the
production of a formulation for personal care products (for example
shampoo; soap; skin, hand and face lotions; skin, hand and face
cremes; hair dyes; toothpaste), food (for example in the baking
industry), detergents and for the production of pharmaceuticals,
e.g. vaccines.
[0026] Yet another aspect is a DNA molecule encoding a protein
variant as defined above.
[0027] Further aspects are a vector comprising a DNA molecule as
described above as well a host cell comprising said DNA
molecule.
[0028] Another aspect is a method of producing a protein variant
having modified immunogenicity as compared to the parent protein as
defined above.
DEFINITIONS
[0029] Prior to a discussion of the detailed embodiments of the
invention, a definition of specific terms related to the main
aspects of the invention is provided.
[0030] In accordance with the present invention there may be
employed conventional molecular biology, microbiology, and
recombinant DNA techniques within the skill of the art. Such
techniques are explained fully in the literature. See, e.g.,
Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory
Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, N.Y. (herein "Sambrook et al., 1989") DNA
Cloning: A Practical Approach, Volumes I and II/D. N. Glover ed.
1985); Oligonucleotide Synthesis (M. J. Gait ed. 1984); Nucleic
Acid Hybridization (B. D. Hames & S. J. Higgins eds (1985));
Transcription And Translation (B. D. Hames & S. J. Higgins,
eds. (1984)); Animal Cell Culture (R. I. Freshney, ed. (1986));
Immobilized Cells And Enzymes (IRL Press, (1986)); B. Perbal, A
Practical Guide To Molecular Cloning (1984).
[0031] When applied to a protein, the term "isolated" indicates
that the protein is found in a condition other than its native
environment, such as apart from blood and animal tissue. In a
preferred form, the isolated protein is substantially free of other
proteins, particularly other proteins of animal origin. It is
preferred to provide the proteins in a highly purified form, i.e.,
greater than 95% pure, more preferably greater than 99% pure. When
applied to a polynucleotide molecule, the term "isolated" indicates
that the molecule is removed from its natural genetic milieu, and
is thus free of other extraneous or unwanted coding sequences, and
is in a form suitable for use within genetically engineered protein
production systems. Such isolated molecules are those that are
separated from their natural environment and include cDNA and
genomic clones. Isolated DNA molecules of the present invention are
free of other genes with which they are ordinarily associated, and
may include naturally occurring 5' and 3' untranslated regions such
as promoters and terminators. The identification of associated
regions will be evident to one of ordinary skill in the art (see
for example, Dynan and Tijan, Nature 316: 774-78, 1985).
[0032] A "polynucleotide" is a single- or double-stranded polymer
of deoxyribonucleotide or ribonucleotide bases read from the 5' to
the 3' end. Polynucleotides include RNA and DNA, and may be
isolated from natural sources, synthesized in vitro, or prepared
from a combination of natural and synthetic molecules.
[0033] A "nucleic acid molecule" refers to the phosphate ester
polymeric form of ribonucleosides (adenosine, guanosine, uridine or
cytidine; "RNA molecules") or deoxyribonucleosides (deoxyadenosine,
deoxyguanosine, deoxythymidine, or deoxycytidine; "DNA molecules")
in either single stranded form, or a double-stranded helix. Double
stranded DNA-DNA, DNA-RNA and RNA-RNA helices are possible. The
term nucleic acid molecule, and in particular DNA or RNA molecule,
refers only to the primary and secondary structure of the molecule,
and does not limit it to any particular tertiary or quaternary
forms. Thus, this term includes double-stranded DNA found, inter
alia, in linear or circular DNA molecules (e.g., restriction
fragments), plasmids, and chromosomes. In discussing the structure
of particular double-stranded DNA molecules, sequences may be
described herein according to the normal convention of giving only
the sequence in the 5' to 3' direction along the nontranscribed
strand of DNA (i.e., the strand having a sequence homologous to the
mRNA). A "recombinant DNA molecule" is a DNA molecule that has
undergone a molecular biological manipulation.
[0034] A DNA "coding sequence" is a double-stranded DNA sequence,
which is transcribed and translated into a polypeptide in a cell in
vitro or in vivo when placed under the control of appropriate
regulatory sequences. The boundaries of the coding sequence are
determined by a start codon at the 5' (amino) terminus and a
translation stop codon at the 3' (carboxyl) terminus. A coding
sequence can include, but is not limited to, prokaryotic sequences,
cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic
(e.g., mammalian) DNA, and even synthetic DNA sequences. If the
coding sequence is intended for expression in a eukaryotic cell, a
polyadenylation signal and transcription termination sequence will
usually be located 3' to the coding sequence.
[0035] An "Expression vector" is a DNA molecule, linear or
circular, that comprises a segment encoding a polypeptide of
interest operably linked to additional segments that provide for
its transcription. Such additional segments may include promoter
and terminator sequences, and optionally one or more origins of
replication, one or more selectable markers, an enhancer, a
polyadenylation signal, and the like. Expression vectors are
generally derived from plasmid or viral DNA, or may contain
elements of both.
[0036] Transcriptional and translational control sequences are DNA
regulatory sequences, such as promoters, enhancers, terminators,
and the like, that provide for the expression of a coding sequence
in a host cell. In eukaryotic cells, polyadenylation signals are
control sequences.
[0037] A "secretory signal sequence" is a DNA sequence that encodes
a polypeptide (a "secretory peptide" that, as a component of a
larger polypeptide, directs the larger polypeptide through a
secretory pathway of a cell in which it is synthesized. The larger
polypeptide is commonly cleaved to remove the secretory peptide
during transit through the secretory pathway.
[0038] The term "promoter" is used herein for its art-recognized
meaning to denote a portion of a gene containing DNA sequences that
provide for the binding of RNA polymerase and initiation of
transcription. Promoter sequences are commonly, but not always,
found in the 5' non-coding regions of genes.
[0039] "Operably linked", when referring to DNA segments, indicates
that the segments are arranged so that they function in concert for
their intended purposes, e.g. transcription initiates in the
promoter and proceeds through the coding segment to the
terminator.
[0040] A coding sequence is "under the control" of transcriptional
and translational control sequences in a cell when RNA polymerase
transcribes the coding sequence into mRNA, which is then trans-RNA
spliced and translated into the protein encoded by the coding
sequence.
[0041] "Isolated polypeptide" is a polypeptide which is essentially
free of other non-[enzyme]polypeptides, e.g., at least about 20%
pure, preferably at least about 40% pure, more preferably about 60%
pure, even more preferably about 80% pure, most preferably about
90% pure, and even most preferably about 95% pure, as determined by
SDS-PAGE.
[0042] "Heterologous" DNA refers to DNA not naturally located in
the cell, or in a chromosomal site of the cell. Preferably, the
heterologous DNA includes a gene foreign to the cell.
[0043] A cell has been "transfected" by exogenous or heterologous
DNA when such DNA has been introduced inside the cell. A cell has
been "transformed" by exogenous or heterologous DNA when the
transfected DNA effects a phenotypic change. Preferably, the
transforming DNA should be integrated (covalently linked) into
chromosomal DNA making up the genome of the cell.
[0044] A "clone" is a population of cells derived from a single
cell or common ancestor by mitosis.
[0045] "Homologous recombination" refers to the insertion of a
foreign DNA sequence of a vector in a chromosome. Preferably, the
vector targets a specific chromosomal site for homologous
recombination. For specific homologous recombination, the vector
will contain sufficiently long regions of homology to sequences of
the chromosome to allow complementary binding and incorporation of
the vector into the chromosome. Longer regions of homology, and
greater degrees of sequence similarity, may increase the efficiency
of homologous recombination.
Nucleic Acid Sequence
[0046] The techniques used to isolate or clone a nucleic acid
sequence encoding a polypeptide are known in the art and include
isolation from genomic DNA, preparation from cDNA, or a combination
thereof. The cloning of the nucleic acid sequences of the present
invention from such genomic DNA can be effected, e.g., by using the
well known polymerase chain reaction (PCR) or antibody screening of
expression libraries to detect cloned DNA fragments with shared
structural features. See, e.g., Innis et al., 1990, A Guide to
Methods and Application, Academic Press, New York. Other nucleic
acid amplification procedures such as ligase chain reaction (LCR),
ligated activated transcription (LAT) and nucleic acid
sequence-based amplification (NASBA) may be used. The nucleic acid
sequence may be cloned from a strain producing the polypeptide, or
from another related organism and thus, for example, may be an
allelic or species variant of the polypeptide encoding region of
the nucleic acid sequence.
[0047] The term "isolated" nucleic acid sequence as used herein
refers to a nucleic acid sequence which is essentially free of
other nucleic acid sequences, e.g., at least about 20% pure,
preferably at least about 40% pure, more preferably about 60% pure,
even more preferably about 80% pure, most preferably about 90%
pure, and even most preferably about 95% pure, as determined by
agarose gel electorphoresis. For example, an isolated nucleic acid
sequence can be obtained by standard cloning procedures used in
genetic engineering to relocate the nucleic acid sequence from its
natural location to a different site where it will be reproduced.
The cloning procedures may involve excision and isolation of a
desired nucleic acid fragment comprising the nucleic acid sequence
encoding the polypeptide, insertion of the fragment into a vector
molecule, and incorporation of the recombinant vector into a host
cell where multiple copies or clones of the nucleic acid sequence
will be replicated. The nucleic acid sequence may be of genomic,
cDNA, RNA, semisynthetic, synthetic origin, or any combinations
thereof.
Nucleic Acid Construct
[0048] As used herein the term "nucleic acid construct" is intended
to indicate any nucleic acid molecule of cDNA, genomic DNA,
synthetic DNA or RNA origin. The term "construct" is intended to
indicate a nucleic acid segment which may be single- or
double-stranded, and which may be based on a complete or partial
naturally occurring nucleotide sequence encoding a polypeptide of
interest. The construct may optionally contain other nucleic acid
segments.
[0049] The DNA of interest may suitably be of genomic or cDNA
origin, for instance obtained by preparing a genomic or cDNA
library and screening for DNA sequences coding for all or part of
the polypeptide by hybridization using synthetic oligonucleotide
probes in accordance with standard techniques (cf. Sambrook et al.,
supra).
[0050] The nucleic acid construct may also be prepared
synthetically by established standard methods, e.g. the
phosphoamidite method described by Beaucage and Caruthers,
Tetrahedron Letters 22 (1981), 1859-1869, or the method described
by Matthes et al., EMBO Journal 3 (1984), 801-805. According to the
phosphoamidite method, oligonucleotides are synthesized, e.g. in an
automatic DNA synthesizer, purified, annealed, ligated and cloned
in suitable vectors.
[0051] Furthermore, the nucleic acid construct may be of mixed
synthetic and genomic, mixed synthetic and cDNA or mixed genomic
and cDNA origin prepared by ligating fragments of synthetic,
genomic or cDNA origin (as appropriate), the fragments
corresponding to various parts of the entire nucleic acid
construct, in accordance with standard techniques.
[0052] The nucleic acid construct may also be prepared by
polymerase chain reaction using specific primers, for instance as
described in U.S. Pat. No. 4,683,202 or Saiki et al., Science 239
(1988), 487-491.
[0053] The term nucleic acid construct may be synonymous with the
term expression cassette when the nucleic acid construct contains
all the control sequences required for expression of a coding
sequence of the present invention. The term "coding sequence" as
defined herein is a sequence which is transcribed into mRNA and
translated into a polypeptide of the present invention when placed
under the control of the above mentioned control sequences. The
boundaries of the coding sequence are generally determined by a
translation start codon ATG at the 5'-terminus and a translation
stop codon at the 3'-terminus. A coding sequence can include, but
is not limited to, DNA, cDNA, and recombinant nucleic acid
sequences.
[0054] The term "control sequences" is defined herein to include
all components which are necessary or advantageous for expression
of the coding sequence of the nucleic acid sequence. Each control
sequence may be native or foreign to the nucleic acid sequence
encoding the polypeptide. Such control sequences include, but are
not limited to, a leader, a polyadenylation sequence, a propeptide
sequence, a promoter, a signal sequence, and a transcription
terminator. At a minimum, the control sequences include a promoter,
and transcriptional and translational stop signals. The control
sequences may be provided with linkers for the purpose of
introducing specific restriction sites facilitating ligation of the
control sequences with the coding region of the nucleic acid
sequence encoding a polypeptide.
[0055] The control sequence may be an appropriate promoter
sequence, a nucleic acid sequence which is recognized by a host
cell for expression of the nucleic acid sequence. The promoter
sequence contains transcription and translation control sequences
which mediate the expression of the polypeptide. The promoter may
be any nucleic acid sequence which shows transcriptional activity
in the host cell of choice and may be obtained from genes encoding
extracellular or intracellular polypeptides either homologous or
heterologous to the host cell.
[0056] The control sequence may also be a suitable transcription
terminator sequence, a sequence recognized by a host cell to
terminate transcription. The terminator sequence is operably linked
to the 3' terminus of the nucleic acid sequence encoding the
polypeptide. Any terminator which is functional in the host cell of
choice may be used in the present invention.
[0057] The control sequence may also be a polyadenylation sequence,
a sequence which is operably linked to the 3' terminus of the
nucleic acid sequence and which, when transcribed, is recognized by
the host cell as a signal to add polyadenosine residues to
transcribed mRNA. Any polyadenylation sequence which is functional
in the host cell of choice may be used in the present
invention.
[0058] The control sequence may also be a signal peptide coding
region, which codes for an amino acid sequence linked to the amino
terminus of the polypeptide which can direct the expressed
polypeptide into the cell's secretory pathway of the host cell. The
5' end of the coding sequence of the nucleic acid sequence may
inherently contain a signal peptide coding region naturally linked
in translation reading frame with the segment of the coding region
which encodes the secreted polypeptide.
[0059] Alternatively, the 5' end of the coding sequence may contain
a signal peptide coding region which is foreign to that portion of
the coding sequence which encodes the secreted polypeptide. A
foreign signal peptide coding region may be required where the
coding sequence does not normally contain a signal peptide coding
region. Alternatively, the foreign signal peptide coding region may
simply replace the natural signal peptide coding region in order to
obtain enhanced secretion relative to the natural signal peptide
coding region normally associated with the coding sequence. The
signal peptide coding region may be obtained from a glucoamylase or
an amylase gene from an Aspergillus species, a lipase or proteinase
gene from a Rhizomucor species, the gene for the alpha-factor from
Saccharomyces cerevisiae, an amylase or a protease gene from a
Bacillus species, or the calf preprochymosin gene. However, any
signal peptide coding region capable of directing the expressed
polypeptide into the secretory pathway of a host cell of choice may
be used in the present invention.
[0060] The control sequence may also be a propeptide coding region,
which codes for an amino acid sequence positioned at the amino
terminus of a polypeptide. The resultant polypeptide is known as a
proenzyme or propolypeptide (or a zymogen in some cases). A
propolypeptide is generally inactive and can be converted to mature
active polypeptide by catalytic or autocatalytic cleavage of the
propeptide from the propolypeptide. The propeptide coding region
may be obtained from the Bacillus subtilis alkaline protease gene
(aprE), the Bacillus subtilis neutral protease gene (nprT), the
Saccharomyces cerevisiae alpha-factor gene, or the Myceliophthora
thermophilum laccase gene (WO 95/33836).
[0061] The nucleic acid constructs of the present invention may
also comprise one or more nucleic acid sequences which encode one
or more factors that are advantageous in the expression of the
polypeptide, e.g., an activator (e.g., a trans-acting factor), a
chaperone, and a processing protease. Any factor that is functional
in the host cell of choice may be used in the present invention.
The nucleic acids encoding one or more of these factors are not
necessarily in tandem with the nucleic acid sequence encoding the
polypeptide.
[0062] An activator is a protein which activates transcription of a
nucleic acid sequence encoding a polypeptide (Kudla et al., 1990,
EMBO Journal 9:1355-1364; Jarai and Buxton, 1994, Current Genetics
26:2238-244; Verdier, 1990, Yeast 6:271-297). The nucleic acid
sequence encoding an activator may be obtained from the genes
encoding Bacillus stearothermophilus NprA (nprA), Saccharomyces
cerevisiae heme activator protein 1 (hap1), Saccharomyces
cerevisiae galactose metabolizing protein 4 (gal4), and Aspergillus
nidulans ammonia regulation protein (areA). For further examples,
see Verdier, 1990, supra and MacKenzie et al., 1993, Journal of
General Microbiology 139:2295-2307.
[0063] A chaperone is a protein which assists another polypeptide
in folding properly (Hartl et al., 1994, TIBS 19:20-25; Bergeron et
al., 1994, TIBS 19:124-128; Demolder et al., 1994, Journal of
Biotechnology 32:179-189; Craig, 1993, Science 260:1902-1903;
Gething and Sambrook, 1992, Nature 355:33-45; Puig and Gilbert,
1994, Journal of Biological Chemistry 269:7764-7771; Wang and Tsou,
1993, The FASEB Journal 7:1515-11157; Robinson et al., 1994,
Bio/Technology 1:381-384). The nucleic acid sequence encoding a
chaperone may be obtained from the genes encoding Bacillus subtilis
GroE proteins, Aspergillus oryzae protein disulphide isomerase,
Saccharomyces cerevisiae calnexin, Saccharomyces cerevisiae
BiP/GRP78, and Saccharomyces cerevisiae Hsp70. For further
examples, see Gething and Sambrook, 1992, supra, and Hartl et al.,
1994, supra.
[0064] A processing protease is a protease that cleaves a
propeptide to generate a mature biochemically active polypeptide
(Enderlin and Ogrydziak, 1994, Yeast 10:67-79; Fuller et al., 1989,
Proceedings of the National Academy of Sciences USA 86:1434-1438;
Julius et al., 1984, Cell 37:1075-1089; Julius et al., 1983, Cell
32:839-852). The nucleic acid sequence encoding a processing
protease may be obtained from the genes encoding Aspergillus niger
Kex2, Saccharomyces cerevisiae dipeptidylaminopeptidase,
Saccharomyces cerevisiae Kex2, and Yarrowia lipolytica dibasic
processing endoprotease (xpr6).
[0065] It may also be desirable to add regulatory sequences which
allow the regulation of the expression of the polypeptide relative
to the growth of the host cell. Examples of regulatory systems are
those which cause the expression of the gene to be turned on or off
in response to a chemical or physical stimulus, including the
presence of a regulatory compound. Regulatory systems in
prokaryotic systems would include the lac, tac, and trp operator
systems. In yeast, the ADH2 system or GAL1 system may be used. In
filamentous fungi, the TAKA alpha-amylase promoter, Aspergillus
niger glucoamylase promoter, and the Aspergillus oryzae
glucoamylase promoter may be used as regulatory sequences. Other
examples of regulatory sequences are those which allow for gene
amplification. In eukaryotic systems, these include the
dihydrofolate reductase gene which is amplified in the presence of
methotrexate, and the metallothionein genes which are amplified
with heavy metals. In these cases, the nucleic acid sequence
encoding the polypeptide would be placed in tandem with the
regulatory sequence.
Promoters
[0066] Examples of suitable promoters for directing the
transcription of the nucleic acid constructs of the present
invention, especially in a bacterial host cell, are the promoters
obtained from the E. coli lac operon, the Streptomyces coelicolor
agarase gene (dagA), the Bacillus subtilis levansucrase gene
(sacB), the Bacillus subtilis alkaline protease gene, the Bacillus
licheniformis alpha-amylase gene (amyL), the Bacillus
stearothermophilus maltogenic amylase gene (amyM), the Bacillus
amyloliquefaciens alpha-amylase gene (amyQ), the Bacillus
amyloliquefaciens BAN amylase gene, the Bacillus licheniformis
penicillinase gene (penP), the Bacillus subtilis xylA and xylB
genes, and the prokaryotic beta-lactamase gene (Villa-Kamaroff et
al., 1978, Proceedings of the National Academy of Sciences USA
75:3727-3731), as well as the tac promoter (DeBoer et al., 1983,
Proceedings of the National Academy of Sciences USA 80:21-25), or
the Bacillus pumilus xylosidase gene, or by the phage Lambda PR or
PL promoters or the E. coli lac, trp or tac promoters. Further
promoters are described in "Useful proteins from recombinant
bacteria" in Scientific American, 1980, 242:74-94; and in Sambrook
et al., 1989, supra.
[0067] Examples of suitable promoters for directing the
transcription of the nucleic acid constructs of the present
invention in a filamentous fungal host cell are promoters obtained
from the genes encoding Aspergillus oryzae TAKA amylase, Rhizomucor
miehei aspartic proteinase, Aspergillus niger neutral
alpha-amylase, Aspergillus niger acid stable alpha-amylase,
Aspergillus niger or Aspergillus awamori glucoamylase (glaA),
Rhizomucor miehei lipase, Aspergillus oryzae alkaline protease,
Aspergillus oryzae triose phosphate isomerase, Aspergillus nidulans
acetamidase, Fusarium oxysporum trypsin-like protease (as described
in U.S. Pat. No. 4,288,627, which is incorporated herein by
reference), and hybrids thereof. Particularly preferred promoters
for use in filamentous fungal host cells are the TAKA amylase,
NA2-tpi (a hybrid of the promoters from the genes encoding
Aspergillus niger neutral alpha-amylase and Aspergillus oryzae
triose phosphate isomerase), and glaA promoters. Further suitable
promoters for use in filamentous fungus host cells are the ADH3
promoter (McKnight et al., The EMBO J. 4 (1985), 2093-2099) or the
tpiA promoter.
[0068] Examples of suitable promoters for use in yeast host cells
include promoters from yeast glycolytic genes (Hitzeman et al., J.
Biol. Chem. 255 (1980), 12073-12080; Alber and Kawasaki, J. Mol.
Appl. Gen. 1 (1982), 419-434) or alcohol dehydrogenase genes (Young
et al., in Genetic Engineering of Microorganisms for Chemicals
(Hollaender et al, eds.), Plenum Press, New York, 1982), or the
TPI1 (U.S. Pat. No. 4,599,311) or ADH2-4-c (Russell et al., Nature
304 (1983), 652-654) promoters.
[0069] Further useful promoters are obtained from the Saccharomyces
cerevisiae enolase (ENO-1) gene, the Saccharomyces cerevisiae
galactokinase gene (GAL1), the Saccharomyces cerevisiae alcohol
dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase genes
(ADH2/GAP), and the Saccharomyces cerevisiae 3-phosphoglycerate
kinase gene. Other useful promoters for yeast host cells are
described by Romanos et al., 1992, Yeast 8:423-488. In a mammalian
host cell, useful promoters include viral promoters such as those
from Simian Virus 40 (SV40), Rous sarcoma virus (RSV), adenovirus,
and bovine papilloma virus (BPV).
[0070] Examples of suitable promoters for directing the
transcription of the DNA encoding the polypeptide of the invention
in mammalian cells are the SV40 promoter (Subramani et al., Mol.
Cell. Biol. 1 (1981), 854-864), the MT-1 (metallothionein gene)
promoter (Palmiter et al., Science 222 (1983), 809-814) or the
adenovirus 2 major late promoter.
[0071] An example of a suitable promoter for use in insect cells is
the polyhedrin promoter (U.S. Pat. No. 4,745,051; Vasuvedan et al.,
FEBS Lett. 311, (1992) 7-11), the P10 promoter (J. M. Vlak et al.,
J. Gen. Virology 69, 1988, pp. 765-776), the Autographa californica
polyhedrosis virus basic protein promoter (EP 397 485), the
baculovirus immediate early gene 1 promoter (U.S. Pat. No.
5,155,037; U.S. Pat. No. 5,162,222), or the baculovirus 39K
delayed-early gene promoter (U.S. Pat. No. 5,155,037; U.S. Pat. No.
5,162,222).
Terminators
[0072] Preferred terminators for filamentous fungal host cells are
obtained from the genes encoding Aspergillus oryzae TAKA amylase,
Aspergillus niger glucoamylase, Aspergillus nidulans anthranilate
synthase, Aspergillus niger alpha-glucosidase, and Fusarium
oxysporum trypsin-like protease. for fungal hosts) the TPI1 (Alber
and Kawasaki, op. cit.) or ADH3 (McKnight et al., op. cit.)
terminators.
[0073] Preferred terminators for yeast host cells are obtained from
the genes encoding Saccharomyces cerevisiae enolase, Saccharomyces
cerevisiae cytochrome C (CYC1), or Saccharomyces cerevisiae
glyceraldehyde-3-phosphate dehydrogenase. Other useful terminators
for yeast host cells are described by Romanos et al., 1992,
supra.
Polyadenylation Signals
[0074] Preferred polyadenylation sequences for filamentous fungal
host cells are obtained from the genes encoding Aspergillus oryzae
TAKA amylase, Aspergillus niger glucoamylase, Aspergillus nidulans
anthranilate synthase, and Aspergillus niger alpha-glucosidase.
[0075] Useful polyadenylation sequences for yeast host cells are
described by Guo and Sherman, 1995, Molecular Cellular Biology
15:5983-5990.
[0076] Polyadenylation sequences are well known in the art for
mammalian host cells such as SV40 or the adenovirus 5 Elb
region.
Signal Sequences
[0077] An effective signal peptide coding region for bacterial host
cells is the signal peptide coding region obtained from the
maltogenic amylase gene from Bacillus NCIB 11837, the Bacillus
stearothermophilus alpha-amylase gene, the Bacillus licheniformis
subtilisin gene, the Bacillus licheniformis beta-lactamase gene,
the Bacillus stearothermophilus neutral proteases genes (nprT,
nprS, nprM), and the Bacillus subtilis PrsA gene. Further signal
peptides are described by Simonen and Palva, 1993, Microbiological
Reviews 57:109-137.
[0078] An effective signal peptide coding region for filamentous
fungal host cells is the signal peptide coding region obtained from
Aspergillus oryzae TAKA amylase gene, Aspergillus niger neutral
amylase gene, the Rhizomucor miehei aspartic proteinase gene, the
Humicola lanuginosa cellulase or lipase gene, or the Rhizomucor
miehei lipase or protease gene, Aspergillus sp. amylase or
glucoamylase, a gene encoding a Rhizomucor miehei lipase or
protease. The signal peptide is preferably derived from a gene
encoding A. oryzae TAKA amylase, A. niger neutral alpha-amylase, A.
niger acid-stable amylase, or A. niger glucoamylase.
[0079] Useful signal peptides for yeast host cells are obtained
from the genes for Saccharomyces cerevisiae alpha-factor and
Saccharomyces cerevisiae invertase. Other useful signal peptide
coding regions are described by Romanos et al., 1992, supra.
[0080] For secretion from yeast cells, the secretory signal
sequence may encode any signal peptide which ensures efficient
direction of the expressed polypeptide into the secretory pathway
of the cell. The signal peptide may be naturally occurring signal
peptide, or a functional part thereof, or it may be a synthetic
peptide. Suitable signal peptides have been found to be the
a-factor signal peptide (cf. U.S. Pat. No. 4,870,008), the signal
peptide of mouse salivary amylase (cf. O. Hagenbuchle et al.,
Nature 289, 1981, pp. 643-646), a modified carboxypeptidase signal
peptide (cf. L. A. Valls et al., Cell 48, 1987, pp. 887-897), the
yeast BAR1 signal peptide (cf. WO 87/02670), or the yeast aspartic
protease 3 (YAP3) signal peptide (cf. M. Egel-Mitani et al., Yeast
6, 1990, pp. 127-137).
[0081] For efficient secretion in yeast, a sequence encoding a
leader peptide may also be inserted downstream of the signal
sequence and upstream of the DNA sequence encoding the polypeptide.
The function of the leader peptide is to allow the expressed
polypeptide to be directed from the endoplasmic reticulum to the
Golgi apparatus and further to a secretory vesicle for secretion
into the culture medium (i.e. exportation of the polypeptide across
the cell wall or at least through the cellular membrane into the
periplasmic space of the yeast cell). The leader peptide may be the
yeast a-factor leader (the use of which is described in e.g. U.S.
Pat. No. 4,546,082, EP 16 201, EP 123 294, EP 123 544 and EP 163
529). Alternatively, the leader peptide may be a synthetic leader
peptide, which is to say a leader peptide not found in nature.
Synthetic leader peptides may, for instance, be constructed as
described in WO 89/02463 or WO 92/11378.
[0082] For use in insect cells, the signal peptide may conveniently
be derived from an insect gene (cf. WO 90/05783), such as the
lepidopteran Manduca sexta adipokinetic hormone precursor signal
peptide (cf. U.S. Pat. No. 5,023,328).
Expression Vectors
[0083] The present invention also relates to recombinant expression
vectors comprising a nucleic acid sequence of the present
invention, a promoter, and transcriptional and translational stop
signals. The various nucleic acid and control sequences described
above may be joined together to produce a recombinant expression
vector which may include one or more convenient restriction sites
to allow for insertion or substitution of the nucleic acid sequence
encoding the polypeptide at such sites. Alternatively, the nucleic
acid sequence of the present invention may be expressed by
inserting the nucleic acid sequence or a nucleic acid construct
comprising the sequence into an appropriate vector for expression.
In creating the expression vector, the coding sequence is located
in the vector so that the coding sequence is operably linked with
the appropriate control sequences for expression, and possibly
secretion.
[0084] The recombinant expression vector may be any vector (e.g., a
plasmid or virus) which can be conveniently subjected to
recombinant DNA procedures and can bring about the expression of
the nucleic acid sequence. The choice of the vector will typically
depend on the compatibility of the vector with the host cell into
which the vector is to be introduced. The vectors may be linear or
closed circular plasmids. The vector may be an autonomously
replicating vector, i.e., a vector which exists as an
extrachromosomal entity, the replication of which is independent of
chromosomal replication, e.g., a plasmid, an extrachromosomal
element, a minichromosome, or an artificial chromosome. The vector
may contain any means for assuring self-replication. Alternatively,
the vector may be one which, when introduced into the host cell, is
integrated into the genome and replicated together with the
chromosome(s) into which it has been integrated. The vector system
may be a single vector or plasmid or two or more vectors or
plasmids which together contain the total DNA to be introduced into
the genome of the host cell, or a transposon.
[0085] The vectors of the present invention preferably contain one
or more selectable markers which permit easy selection of
transformed cells. A selectable marker is a gene the product of
which provides for biocide or viral resistance, resistance to heavy
metals, prototrophy to auxotrophs, and the like. Examples of
bacterial selectable markers are the dal genes from Bacillus
subtilis or Bacillus licheniformis, or markers which confer
antibiotic resistance such as ampicillin, kanamycin,
chloramphenicol, tetracycline, neomycin, hygromycin or methotrexate
resistance. A frequently used mammalian marker is the dihydrofolate
reductase gene (DHFR). Suitable markers for yeast host cells are
ADE2, HIS3, LEU2, LYS2, MET3, TRP1, and URA3. A selectable marker
for use in a filamentous fungal host cell may be selected from the
group including, but not limited to, amdS (acetamidase), argB
(ornithine carbamoyltransferase), bar (phosphinothricin
acetyltransferase), hygB (hygromycin phosphotransferase), niaD
(nitrate reductase), pyrG (orotidine-5'-phosphate decarboxylase),
sC (sulfate adenyltransferase), trpC (anthranilate synthase), and
glufosinate resistance markers, as well as equivalents from other
species. Preferred for use in an Aspergillus cell are the amdS and
pyrG markers of Aspergillus nidulans or Aspergillus oryzae and the
bar marker of Streptomyces hygroscopicus. Furthermore, selection
may be accomplished by co-transformation, e.g., as described in WO
91/17243, where the selectable marker is on a separate vector.
[0086] The vectors of the present invention preferably contain an
element(s) that permits stable integration of the vector into the
host cell genome or autonomous replication of the vector in the
cell independent of the genome of the cell.
[0087] The vectors of the present invention may be integrated into
the host cell genome when introduced into a host cell. For
integration, the vector may rely on the nucleic acid sequence
encoding the polypeptide or any other element of the vector for
stable integration of the vector into the genome by homologous or
nonhomologous recombination. Alternatively, the vector may contain
additional nucleic acid sequences for directing integration by
homologous recombination into the genome of the host cell. The
additional nucleic acid sequences enable the vector to be
integrated into the host cell genome at a precise location(s) in
the chromosome(s). To increase the likelihood of integration at a
precise location, the integrational elements should preferably
contain a sufficient number of nucleic acids, such as 100 to 1,500
base pairs, preferably 400 to 1,500 base pairs, and most preferably
800 to 1,500 base pairs, which are highly homologous with the
corresponding target sequence to enhance the probability of
homologous recombination. The integrational elements may be any
sequence that is homologous with the target sequence in the genome
of the host cell. Furthermore, the integrational elements may be
non-encoding or encoding nucleic acid sequences. On the other hand,
the vector may be integrated into the genome of the host cell by
non-homologous recombination. These nucleic acid sequences may be
any sequence that is homologous with a target sequence in the
genome of the host cell, and, furthermore, may be non-encoding or
encoding sequences.
[0088] For autonomous replication, the vector may further comprise
an origin of replication enabling the vector to replicate
autonomously in the host cell in question. Examples of bacterial
origins of replication are the origins of replication of plasmids
pBR322, pUC19, pACYC177, pACYC184, pUB110, pE194, pTA1060, and
pAM.beta.1. Examples of origin of replications for use in a yeast
host cell are the 2 micron origin of replication, the combination
of CEN6 and ARS4, and the combination of CEN3 and ARS1. The origin
of replication may be one having a mutation which makes its
functioning temperature-sensitive in the host cell (see, e.g.,
Ehrlich, 1978, Proceedings of the National Academy of Sciences USA
75:1433).
[0089] More than one copy of a nucleic acid sequence encoding a
polypeptide of the present invention may be inserted into the host
cell to amplify expression of the nucleic acid sequence. Stable
amplification of the nucleic acid sequence can be obtained by
integrating at least one additional copy of the sequence into the
host cell genome using methods well known in the art and selecting
for transformants.
[0090] The procedures used to ligate the elements described above
to construct the recombinant expression vectors of the present
invention are well known to one skilled in the art (see, e.g.,
Sambrook et al., 1989, supra).
Host Cells
[0091] The present invention also relates to recombinant host
cells, comprising a nucleic acid sequence of the invention, which
are advantageously used in the recombinant production of the
polypeptides. The term "host cell" encompasses any progeny of a
parent cell which is not identical to the parent cell due to
mutations that occur during replication.
[0092] The cell is preferably transformed with a vector comprising
a nucleic acid sequence of the invention followed by integration of
the vector into the host chromosome. "Transformation" means
introducing a vector comprising a nucleic acid sequence of the
present invention into a host cell so that the vector is maintained
as a chromosomal integrant or as a self-replicating
extra-chromosomal vector. Integration is generally considered to be
an advantage as the nucleic acid sequence is more likely to be
stably maintained in the cell. Integration of the vector into the
host chromosome may occur by homologous or non-homologous
recombination as described above.
[0093] The choice of a host cell will to a large extent depend upon
the gene encoding the polypeptide and its source. The host cell may
be a unicellular microorganism, e.g., a prokaryote, or a
non-unicellular microorganism, e.g., a eukaryote. Useful
unicellular cells are bacterial cells such as gram positive
bacteria including, but not limited to, a Bacillus cell, e.g.,
Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis,
Bacillus circulans, Bacillus coagulans, Bacillus lautus, Bacillus
lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus
stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis;
or a Streptomyces cell, e.g., Streptomyces lividans or Streptomyces
murinus, or gram negative bacteria such as E. coli and Pseudomonas
sp. In a preferred embodiment, the bacterial host cell is a
Bacillus lentus, Bacillus licheniformis, Bacillus
stearothermophilus or Bacillus subtilis cell. The transformation of
a bacterial host cell may, for instance, be effected by protoplast
transformation (see, e.g., Chang and Cohen, 1979, Molecular General
Genetics 168:111-115), by using competent cells (see, e.g., Young
and Spizizin, 1961, Journal of Bacteriology 81:823-829, or Dubnar
and Davidoff-Abelson, 1971, Journal of Molecular Biology
56:209-221), by electroporation (see, e.g., Shigekawa and Dower,
1988, Biotechniques 6:742-751), or by conjugation (see, e.g.,
Koehler and Thorne, 1987, Journal of Bacteriology
169:5771-5278).
[0094] The host cell may be a eukaryote, such as a mammalian cell,
an insect cell, a plant cell or a fungal cell.
[0095] Useful mammalian cells include Chinese hamster ovary (CHO)
cells, HeLa cells, baby hamster kidney (BHK) cells, COS cells, or
any number of other immortalized cell lines available, e.g., from
the American Type Culture Collection.
[0096] Examples of suitable mammalian cell lines are the COS (ATCC
CRL 1650 and 1651), BHK (ATCC CRL 1632, 10314 and 1573, ATCC CCL
10), CHL (ATCC CCL39) or CHO (ATCC CCL 61) cell lines. Methods of
transfecting mammalian cells and expressing DNA sequences
introduced in the cells are described in e.g. Kaufman and Sharp, J.
Mol. Biol. 159 (1982), 601-621; Southern and Berg, J. Mol. Appl.
Genet. 1 (1982), 327-341; Loyter et al., Proc. Natl. Acad. Sci. USA
79 (1982), 422-426; Wigler et al., Cell 14 (1978), 725; Corsaro and
Pearson, Somatic Cell Genetics 7 (1981), 603, Ausubel et al.,
Current Protocols in Molecular Biology, John Wiley and Sons, Inc.,
N.Y., 1987, Hawley-Nelson et al., Focus 15 (1993), 73; Ciccarone et
al., Focus 15 (1993), 80; Graham and van der Eb, Virology 52
(1973), 456; and Neumann et al., EMBO J. 1 (1982), 841-845.
[0097] In a preferred embodiment, the host cell is a fungal cell.
"Fungi" as used herein includes the phyla Ascomycota,
Basidiomycota, Chytridiomycota, and Zygomycota (as defined by
Hawksworth et al., In, Ainsworth and Bisby's Dictionary of The
Fungi, 8th edition, 1995, CAB International, University Press,
Cambridge, UK) as well as the Oomycota (as cited in Hawksworth et
al., 1995, supra, page 171) and all mitosporic fungi (Hawksworth et
al., 1995, supra). Representative groups of Ascomycota include,
e.g., Neurospora, Eupenicillium (=Penicillium), Emericella
(=Aspergillus), Eurotium (=Aspergillus), and the true yeasts listed
above. Examples of Basidiomycota include mushrooms, rusts, and
smuts. Representative groups of Chytridiomycota include, e.g.,
Allomyces, Blastocladiella, Coelomomyces, and aquatic fungi.
Representative groups of Oomycota include, e.g.,
Saprolegniomycetous aquatic fungi (water molds) such as Achlya.
Examples of mitosporic fungi include Aspergillus, Penicillium,
Candida, and Alternaria. Representative groups of Zygomycota
include, e.g., Rhizopus and Mucor.
[0098] In a preferred embodiment, the fungal host cell is a yeast
cell. "Yeast" as used herein includes ascosporogenous yeast
(Endomycetales), basidiosporogenous yeast, and yeast belonging to
the Fungi Imperfecti (Blastomycetes). The ascosporogenous yeasts
are divided into the families Spermophthoraceae and
Saccharomycetaceae. The latter is comprised of four subfamilies,
Schizosaccharomycoideae (e.g., genus Schizosaccharomyces),
Nadsonioideae, Lipomycoideae, and Saccharomycoideae (e.g., genera
Pichia, Kluyveromyces and Saccharomyces). The basidiosporogenous
yeasts include the genera Leucosporidim, Rhodosporidium,
Sporidiobolus, Filobasidium, and Filobasidiella. Yeast belonging to
the Fungi Imperfecti are divided into two families,
Sporobolomycetaceae (e.g., genera Sorobolomyces and Bullera) and
Cryptococcaceae (e.g., genus Candida). Since the classification of
yeast may change in the future, for the purposes of this invention,
yeast shall be defined as described in Biology and Activities of
Yeast (Skinner, F. A., Passmore, S. M., and Davenport, R. R., eds,
Soc. App. Bacteriol. Symposium Series No. 9, 1980. The biology of
yeast and manipulation of yeast genetics are well known in the art
(see, e.g., Biochemistry and Genetics of Yeast, Bacil, M.,
Horecker, B. J., and Stopani, A. O. M., editors, 2nd edition, 1987;
The Yeasts, Rose, A. H., and Harrison, J. S., editors, 2nd edition,
1987; and The Molecular Biology of the Yeast Saccharomyces,
Strathern et al., editors, 1981).
[0099] The yeast host cell may be selected from a cell of a species
of Candida, Kluyveromyces, Saccharomyces, Schizosaccharomyces,
Candida, Pichia, Hansenula, or Yarrowia. In a preferred embodiment,
the yeast host cell is a Saccharomyces carlsbergensis,
Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomyces
douglasii, Saccharomyces kluyveri, Saccharomyces norbensis or
Saccharomyces oviformis cell. Other useful yeast host cells are a
Kluyveromyces lactis, Kluyveromyces fragilis, Hansenula polymorpha,
Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe,
Ustilgo maylis, Candida maltose, Pichia guillermondii and Pichia
methanolio cell (cf. Gleeson et al., J. Gen. Microbiol. 132, 1986,
pp. 3459-3465; U.S. Pat. No. 4,882,279 and U.S. Pat. No.
4,879,231).
[0100] In a preferred embodiment, the fungal host cell is a
filamentous fungal cell. "Filamentous fungi" include all
filamentous forms of the subdivision Eumycota and Oomycota (as
defined by Hawksworth et al., 1995, supra). The filamentous fungi
are characterized by a vegetative mycelium composed of chitin,
cellulose, glucan, chitosan, mannan, and other complex
polysaccharides. Vegetative growth is by hyphal elongation and
carbon catabolism is obligately aerobic. In contrast, vegetative
growth by yeasts such as Saccharomyces cerevisiae is by budding of
a unicellular thallus and carbon catabolism may be fermentative. In
a more preferred embodiment, the filamentous fungal host cell is a
cell of a species of, but not limited to, Acremonium, Aspergillus,
Fusarium, Humicola, Mucor, Myceliophthora, Neurospora, Penicillium,
Thielavia, Tolypocladium, and Trichoderma or a teleomorph or
synonym thereof. In an even more preferred embodiment, the
filamentous fungal host cell is an Aspergillus cell. In another
even more preferred embodiment, the filamentous fungal host cell is
an Acremonium cell. In another even more preferred embodiment, the
filamentous fungal host cell is a Fusarium cell. In another even
more preferred embodiment, the filamentous fungal host cell is a
Humicola cell. In another even more preferred embodiment, the
filamentous fungal host cell is a Mucor cell. In another even more
preferred embodiment, the filamentous fungal host cell is a
Myceliophthora cell. In another even more preferred embodiment, the
filamentous fungal host cell is a Neurospora cell. In another even
more preferred embodiment, the filamentous fungal host cell is a
Penicillium cell. In another even more preferred embodiment, the
filamentous fungal host cell is a Thielavia cell. In another even
more preferred embodiment, the filamentous fungal host cell is a
Tolypocladium cell. In another even more preferred embodiment, the
filamentous fungal host cell is a Trichoderma cell. In a most
preferred embodiment, the filamentous fungal host cell is an
Aspergillus awamori, Aspergillus foetidus, Aspergillus japonicus,
Aspergillus niger, Aspergillus nidulans or Aspergillus oryzae cell.
In another most preferred embodiment, the filamentous fungal host
cell is a Fusarium cell of the section Discolor (also known as the
section Fusarium). For example, the filamentous fungal parent cell
may be a Fusarium bactridioides, Fusarium cerealis, Fusarium
crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium
graminum, Fusarium heterosporum, Fusarium negundi, Fusarium
reticulaturn, Fusarium roseum, Fusarium sambucinum, Fusarium
sarcochroum, Fusarium sulphureum, or Fusarium trichothecioides
cell. In another preferred embodiment, the filamentous fungal
parent cell is a Fusarium strain of the section Elegans, e.g.,
Fusarium oxysporum. In another most preferred embodiment, the
filamentous fungal host cell is a Humicola insolens or Humicola
lanuginosa cell. In another most preferred embodiment, the
filamentous fungal host cell is a Mucor miehei cell. In another
most preferred embodiment, the filamentous fungal host cell is a
Myceliophthora thermophilum cell. In another most preferred
embodiment, the filamentous fungal host cell is a Neurospora crassa
cell. In another most preferred embodiment, the filamentous fungal
host cell is a Penicillium purpurogenum cell. In another most
preferred embodiment, the filamentous fungal host cell is a
Thielavia terrestris cell or an Acremonium chtysogenum cell. In
another most preferred embodiment, the Trichoderma cell is a
Trichoderma harzianum, Trichoderma koningii, Trichoderma
longibrachiatum, Trichoderma reesei or Trichoderma viride cell. The
use of Aspergillus spp. for the expression of proteins is described
in, e.g., EP 272 277, EP 230 023.
Transformation
[0101] Fungal cells may be transformed by a process involving
protoplast formation, transformation of the protoplasts, and
regeneration of the cell wall in a manner known per se. Suitable
procedures for transformation of Aspergillus host cells are
described in EP 238 023 and Yelton et al., 1984, Proceedings of the
National Academy of Sciences USA 81:1470-1474. A suitable method of
transforming Fusarium species is described by Malardier et al.,
1989, Gene 78:147-156 or in copending U.S. Ser. No. 08/269,449.
Examples of other fungal cells are cells of filamentous fungi, e.g.
Aspergillus spp., Neurospora spp., Fusarium spp. or Trichoderma
spp., in particular strains of A. oryzae, A. nidulans or A. niger.
The use of Aspergillus spp. for the expression of proteins is
described in, e.g., EP 272 277, EP 230 023. The transformation of
F. oxysporum may, for instance, be carried out as described by
Malardier et al., 1989, Gene 78: 147-156.
[0102] Yeast may be transformed using the procedures described by
Becker and Guarente, In Abelson, J. N. and Simon, M. I., editors,
Guide to Yeast Genetics and Molecular Biology, Methods in
Enzymology, Volume 194, pp 182-187, Academic Press, Inc., New York;
Ito et al., 1983, Journal of Bacteriology 153:163; and Hinnen et
al., 1978, Proceedings of the National Academy of Sciences USA
75:1920. Mammalian cells may be transformed by direct uptake using
the calcium phosphate precipitation method of Graham and Van der Eb
(1978, Virology 52:546).
[0103] Transformation of insect cells and production of
heterologous polypeptides therein may be performed as described in
U.S. Pat. No. 4,745,051; U.S. Pat. No. 4,775,624; U.S. Pat. No.
4,879,236; U.S. Pat. No. 5,155,037; U.S. Pat. No. 5,162,222; EP
397,485) all of which are incorporated herein by reference. The
insect cell line used as the host may suitably be a Lepidoptera
cell line, such as Spodoptera frugiperda cells or Trichoplusia ni
cells (cf. U.S. Pat. No. 5,077,214). Culture conditions may
suitably be as described in, for instance, WO 89/01029 or WO
89/01028, or any of the aforementioned references.
Methods of Production
[0104] The transformed or transfected host cells described above
are cultured in a suitable nutrient medium under conditions
permitting the production of the desired molecules, after which
these are recovered from the cells, or the culture broth.
[0105] The medium used to culture the cells may be any conventional
medium suitable for growing the host cells, such as minimal or
complex media containing appropriate supplements. Suitable media
are available from commercial suppliers or may be prepared
according to published recipes (e.g. in catalogues of the American
Type Culture Collection). The media are prepared using procedures
known in the art (see, e.g., references for bacteria and yeast;
Bennett, J. W. and LaSure, L., editors, More Gene Manipulations in
Fungi, Academic Press, CA, 1991).
[0106] If the molecules are secreted into the nutrient medium, they
can be recovered directly from the medium. If they are not
secreted, they can be recovered from cell lysates. The molecules
are recovered from the culture medium by conventional procedures
including separating the host cells from the medium by
centrifugation or filtration, precipitating the proteinaceous
components of the supernatant or filtrate by means of a salt, e.g.
ammonium sulphate, purification by a variety of chromatographic
procedures, e.g. ion exchange chromatography, gelfiltration
chromatography, affinity chromatography, or the like, dependent on
the type of molecule in question.
[0107] The molecules of interest may be detected using methods
known in the art that are specific for the molecules. These
detection methods may include use of specific antibodies, formation
of a product, or disappearance of a substrate. For example, an
enzyme assay may be used to determine the activity of the molecule.
Procedures for determining various kinds of activity are known in
the art.
[0108] The molecules of the present invention may be purified by a
variety of procedures known in the art including, but not limited
to, chromatography (e.g., ion exchange, affinity, hydrophobic,
chromatofocusing, and size exclusion), electrophoretic procedures
(e.g., preparative isoelectric focusing (IEF), differential
solubility (e.g., ammonium sulfate precipitation), or extraction
(see, e.g., Protein Purification, J-C Janson and Lars Ryden,
editors, VCH Publishers, New York, 1989).
[0109] The term "immunological response", used in connection with
the present invention, is the response of an organism to a
compound, which involves the immune system according to any of the
four standard reactions (Type I, II, III and IV according to Coombs
& Gell).
[0110] Correspondingly, the "immunogenicity" of a compound used in
connection with the present invention refers to the ability of this
compound to induce an `immunological response` in animals including
man.
[0111] The term "allergic response", used in connection with the
present invention, is the response of an organism to a compound,
which involves IgE mediated responses (Type I reaction according to
Coombs & Gell). It is to be understood that sensibilization
(i.e. development of compound-specific IgE antibodies) upon
exposure to the compound is included in the definition of "allergic
response".
[0112] Correspondingly, the "allergenicity" of a compound used in
connection with the present invention refers to the ability of this
compound to induce an `allergic response` in animals including
man.
[0113] The term "parent protein" refer to the polypeptide to be
modified by creating a library of diversified mutants. The "parent
protein" may be a naturally occurring (or wild-type) polypeptide or
it may be a variant thereof prepared by any suitable means. For
instance, the "parent protein" may be a variant of a naturally
occurring polypeptide which has been modified by substitution,
deletion or truncation of one or more amino acid residues or by
addition or insertion of one or more amino acid residues to the
amino acid sequence of a naturally-occurring polypeptide.
[0114] The term "enzyme variants" or "protein variants" refer to a
polypeptide of the invention comprising one or more substitutions
of the specified amino acid residues. The total number of such
substitutions is typically not more than 10, e.g. one, two, three,
four, five or six of said substitutions. In addition, the enzyme
variant or protein variant of the invention may optionally include
other modifications of the parent enzyme, typically not more than
10, e.g. not more than 5 such modifications. The variant generally
has a homology with the parent enzyme of at least 80%, e.g. at
least 85%, typically at least 90% or at least 95%.
[0115] The term "randomized library" of protein variants refers to
a library with at least partially randomized composition of the
members, e.g. protein variants.
[0116] An "epitope" is a set of amino acids on a protein that are
involved in an immunological response, such as antibody binding or
T-cell activation. One particularly useful method of identifying
epitopes involved in antibody binding is to screen a library of
peptide-phage membrane protein fusions and selecting those that
bind to relevant antigen-specific antibodies, sequencing the
randomized part of the fusion gene, aligning the sequences involved
in binding, defining consensus sequences based on these alignments,
and mapping these consensus sequences on the surface or the
sequence and/or structure of the antigen, to identify epitopes
involved in antibody binding.
[0117] By the term "epitope pattern" is meant such a consensus
sequence of antibody binding peptides. An example is the epitope
pattern A R R<R. The sign "<" in this notation indicates that
the aligned antibody binding peptides included a non-consensus
amino acid between the second and the third arginine.
[0118] An "epitope area" is defined as the amino acids situated
close to the epitope sequence amino acids. Preferably, the amino
acids of an epitope area are located <5 .ANG. from the epitope
sequence. Hence, an epitope area also includes the corresponding
epitope sequence itself. Modifications of amino acids of the
`epitope area` can possibly affect the immunogenic function of the
corresponding epitope.
[0119] By the term "epitope sequence" is meant the amino acid
residues of a parent protein, which have been identified to belong
to an epitope by the methods of the present invention (an example
of an epitope sequence is E271 Q12 I8 in Savinase).
[0120] The term `antibody binding peptide` denotes a peptide that
bind with sufficiently high affinity to antibodies. Identification
of `antibody binding peptides` and their sequences constitute the
first step of the method of this invention.
[0121] "Anchor amino acids" are the individual amino acids of an
epitope pattern.
[0122] "Hot spot amino acids" are amino acids of parent protein,
which are particularly likely to result in modified immunogenecity
if they are mutated. Amino acids, which appear in three or more
epitope sequences or which correspond to anchor amino acids are hot
spot amino acids.
[0123] "Environmental allergens" are protein allergens that are
present naturally. They include pollen, dust mite allergens, pet
allergens, food allergens, venoms, etc.
[0124] "Commercial allergens" are protein allergens that are being
brought to the market commercially. They include enzymes,
pharmaceutical proteins, antimicrobial peptides, as well as
allergens of transgenic plants.
[0125] The "donor protein" is the protein that was used to raise
antibodies used to identify antibody binding sequences, hence the
donor protein provides the information that leads to the epitope
patterns.
[0126] The "acceptor protein" is the protein, whose structure is
used to fit the identified epitope patterns and/or to fit the
antibody binding sequences. Hence the acceptor protein is also the
parent protein.
[0127] An "autoepitope" is one that has been identified using
antibodies raised against the parent protein, i.e. the acceptor and
the donor proteins are identical.
[0128] A "heteroepitope" is one that has been identified with
distinct donor and acceptor proteins.
[0129] The term "functionality" of protein variants refers to e.g.
enzymatic activity; binding to a ligand or receptor; stimulation of
a cellular response (e.g. .sup.3H-thymidine incorporation as
response to a mitogenic factor); or anti-microbial activity.
[0130] By the term "specific polyclonal antibodies" is meant
polyclonal antibodies isolated according to their specificity for a
certain antigen, e.g. the protein backbone.
[0131] By the term "monospecific antibodies" is meant polyclonal
antibodies isolated according to their specificity for a certain
epitope. Such monospecific antibodies will bind to the same
epitope, but with different affinity, as they are produced by a
number of antibody producing cells recognizing overlapping but not
necessarily identical epitopes.
[0132] The term "randomized library" of protein variants refers to
a library with at least partially randomized composition of the
members, e.g. protein variants.
[0133] `Spiked mutagenesis` is a form of site-directed mutagenesis,
in which the primers used have been synthesized using mixtures of
oligonucleotides at one or more positions.
[0134] By the term "a protein variant having modified
immunogenicity as compared to the parent protein" is meant a
protein variant which differs from the parent protein in one or
more amino acids whereby the immunogenicity of the variant is
modified. The modification of immunogenicity may be confirmed by
testing the ability of the protein variant to elicit an IgE/IgG
response.
[0135] In the present context the term "protein" is intended to
cover oligopeptides, polypeptides as well as proteins as such.
DETAILED DESCRIPTION OF THE INVENTION
[0136] The present invention relates to a method of selecting a
protein variant having modified immunogenicity as compared to a
parent protein, comprising the steps of:
[0137] a) obtaining antibody binding peptide sequences,
[0138] b) using the sequences to localise epitope sequences on the
3-dimensional structure of parent protein,
[0139] c) defining an epitope area including amino acids situated
within 5 .ANG. from the epitope amino acids constituting the
epitope sequence,
[0140] d) changing one or more of the amino acids defining the
epitope area of the parent protein by genetic engineering mutations
of a DNA sequence encoding the parent protein,
[0141] e) introducing the mutated DNA sequence into a suitable
host, culturing said host and expressing the protein variant,
and
[0142] f) evaluating the immunogenicity of the protein variant
using the parent protein as reference.
A) How to Find Antibody Binding Peptide Sequences and Epitope
Patterns
[0143] A first step of the method is to identify peptide sequences,
which bind specifically to antibodies.
[0144] Antibody binding peptide sequences can be found by testing a
set of known peptide sequences for binding to antibodies raised
against the donor protein. These sequences are typically selected,
such that each represents a segment of the donor protein sequence
(Mol. Immunol., 1992, vol. 29, pp. 1383-1389; Am. J. Resp. Cell.
Mol. Biol. 2000, vol. 22, pp. 344-351). Also, randomized synthetic
peptide libraries can be used to find antibody binding sequences
(Slootstra et al; Molecular Diversity, 1996, vol. 2, pp.
156-164).
[0145] In a preferred method, the identification of antibody
binding sequences may be achieved by screening of a display package
library, preferably a phage display library. The principle behind
phage display is that a heterologous DNA sequence can be inserted
in the gene coding for a coat protein of the phage (WO 92/15679).
The phage will make and display the hybrid protein on its surface
where it can interact with specific target agents. Such target
agent may be antigen-specific antibodies. It is therefore possible
to select specific phages that display antibody-binding peptide
sequences. The displayed peptides can be of predetermined lengths,
for example 9 amino acids long, with randomized sequences,
resulting in a random peptide display package library. Thus, by
screening for antibody binding, one can isolate the peptide
sequences that have sufficiently high affinity for the particular
antibody used. The peptides of the hybrid proteins of the specific
phages which bind protein-specific antibodies characterize epitopes
that are recognized by the immune system.
[0146] The antibodies used for reacting with the display package
are preferably IgE antibodies to ensure that the epitopes
identified are IgE epitopes, i.e. epitopes inducing and binding
IgE. In a preferred embodiment the antibodies are polyclonal
antibodies, optionally monospecific antibodies.
[0147] For the purpose of the present invention polyclonal
antibodies are preferred in order to obtain a broader knowledge
about the epitopes of a protein.
[0148] It is of great importance that the amino acid sequence of
the peptides presented by the display packages is long enough to
represent a significant part of the epitope to be identified. In a
preferred embodiment of the invention the peptides of the peptide
display package library are oligopeptides having from 5 to 25 amino
acids, preferably at least 8 amino acids, such as 9 amino acids.
For a given length of peptide sequences (n), the theoretical number
of different possible sequences can be calculated as 20.sup.n. The
diversity of the package library used must be large enough to
provide a suitable representation of the theoretical number of
different sequences. In a phage-display library, each phage has one
specific sequence of a determined length. Hence an average phage
display library can express 10.sup.8-10.sup.12 different random
sequences, and is therefore well-suited to represent the
theoretical number of different sequences.
[0149] The antibody binding peptide sequences can be further
analysed by consensus alignment e.g. by the methods described by
Feng and Doolittle, Meth. Enzymol., 1996, vol. 266, pp. 368-382;
Feng and Doolittle, J. Mol. Evol., 1987, vol. 25, pp. 351-360; and
Taylor, Meth. Enzymol., 1996, vol. 266, pp. 343-367.
[0150] This leads to identification of epitope patterns, which can
assist the comparison of the linear information obtained from the
antibody binding peptide sequences to the 3-o dimensional structure
of the acceptor protein in order to identify epitope sequences at
the surface of the acceptor protein.
B) How to Identify Epitope Sequences and Epitope Areas.
[0151] Given a number of antibody binding peptide sequences and
possibly the corresponding epitope patterns, one need the
3-dimensional structure coordinates of an acceptor protein to find
the epitope sequences on its surface.
[0152] These coordinates can be found in databases (NCBI:
http://www.ncbi.nlm.nih.gov/), determined experimentally using
conventional methods (Ducruix and Giege: Crystallization of Nucleic
Acids and Proteins, IRL PRess, Oxford, 1992, ISBN 0-19-963245-6),
or they can be deduced from the coordinates of a homologous
protein. Typical actions required for the construction of a model
structure are: alignment of homologous sequences for which
3-dimensional structures exist, definition of Structurally
Conserved Regions (SCRs), assignment of coordinates to SCRs, search
for structural fragments/loops in structure databases to replace
Variable Regions, assignment of coordinates to these regions, and
structural refinement by energy minimization. Regions containing
large inserts (>3 residues) relative to the known 3-dimensional
structures are known to be quite difficult to model, and structural
predictions must be considered with care.
[0153] Using the coordinates and the several methods of mapping the
linear information on the 3-dimensional surface are possible, as
described in the examples below.
[0154] One can match each amino acid residue of the antibody
binding peptide to an identical or homologous amino acid on the 3-D
surface of the acceptor protein, such that amino acids that are
adjacent in the primary sequence are close on the surface of the
acceptor protein, with close being <5 .ANG., preferably <3
.ANG. between any two atoms of the two amino acids.
[0155] Alternatively, one can define a geometric body (e.g. an
ellipsoid, a sphere, or a box) of a size that matches a possible
binding interface between antibody and antigen and look for a
positioning of this body where it will contain most of or all the
anchor amino acids.
[0156] Also, one can use the epitope patterns to facilitate
identification of epitope sequences. This can be done, by first
matching the anchor amino acids on the 3-D structure and
subsequently looking for other elements of the antibody binding
peptide sequences, which provide additional matches. If there are
many residues to be matched, it is only necessary that a suitable
number can be found on the 3-D structure. For example if an epitope
pattern comprises 4, 5, 6, or 7 amino acids, it is only necessary
that 3 matches surface elements of the acceptor protein.
[0157] In all cases, it is desirable that amino acids of the
epitope sequence are surface exposed (as described below in
Examples).
[0158] It is known, that amino acids that surround binding
sequences can affect binding of a ligand without participating
actively in the binding process. Based on this knowledge, areas
covered by amino acids with potential steric effects on the
epitope-antibody interaction, were defined around the identified
epitope sequences. These areas are called `epitope areas`.
Practically, all amino acids situated within 5 .ANG. from the amino
acids defining the epitope sequence were included. Preferably, the
epitope area equals the epitope sequence. The accessibility
criterium was not used as hidden amino acids of an epitope area
also can have an effect on the adjacent amino acids of the epitope
sequence.
C) How to Use the Epitope Information.
[0159] There are at least four ways to utilize the information
about epitope sequences, which has been derived by the methods of
this invention:
[0160] 1) reduce the allergenicity of a commercial protein using
protein engineering.
[0161] 2) reduce the potential of commercial proteins to
cross-react with environmental allergens and hence cause allergic
reactions in people sensitized to the environmental allergens (or
vice versa).
[0162] 3) improve the immunotherapeutic effect of allergen
vaccines.
[0163] 4) assist characterization of clinical allergies in order to
select the appropriate allergen vaccine.
Protein Engineering to Reduce the Allergenicity, Cross-Reactivity
and/or Immunotherapeutic Effect of Proteins.
[0164] The methods described thus far have led to identification of
epitope areas on an acceptor protein, each containing epitope
sequences. These subsets of amino acids, are preferred for
introducing mutations that are meant to modify the immunogenecity
of the acceptor protein. An even more preferred subset of amino
acids to target by mutagenesis are `hot spot amino acids`, which
appear in several different epitope sequences, or which corresponds
to anchor amino acids of the epitope patterns.
[0165] Thus, genetic engineering mutations should be designed in
the epitope areas, preferably in epitope sequences, and more
preferably in the `hot spot amino acids`.
Substitution, Deletion, Insertion
[0166] When the epitope area(s) have been identified, a protein
variant exhibiting a modified immunogenicity may be produced by
changing the identified epitope area of the parent protein by
genetic engineering mutation of a DNA sequence encoding the parent
protein.
[0167] The epitope identified may be changed by substituting at
least one amino acid of the epitope area. In a preferred embodiment
at least one anchor amino acid or hot spot amino acid is changed.
The change will often be substituting to an amino acid of different
size, hydrophilicity, and/or polarity, such as a small amino acid
versus a large amino acid, a hydrophilic amino acid versus a
hydrophobic amino acid, a polar amino acid versus a non-polar amino
acid and a basic versus an acidic amino acid.
[0168] Other changes may be the addition/insertion or deletion of
at least one amino acid of the epitope sequence, preferably
deleting an anchor amino acid or a hot spot amino acid.
Furthermore, an epitope pattern may be changed by substituting some
amino acids, and deleting/adding other.
[0169] In the claims a position to be changed by substitution,
insertion, deletion will be indicated by: "Position xx to aaa, bbb,
ccc, insertion, deletion", meaning that position xx can be
substituted by the amino acid aaa, bbb, ccc or that any amino acid
can be inserted after position xx or that position xx can be
deleted, e.g. "Position 27 to A, D, E, insertion, deletion" means
that in position 27 the amino acid can be substituted by A, D or E,
or that any amino acid can be inserted after position 27, or that
the amino acid in position 27 can be deleted.
[0170] When one uses protein engineering to eliminate epitopes, it
is indeed possible that new epitopes are created, or existing
epitopes are duplicated. To reduce this risk, one can map the
planned mutations at a given position on the 3-dimensional
structure of the protein of interest, and control the emerging
amino acid constellation against a database of known epitope
patterns, to rule out those possible replacement amino acids, which
are predicted to result in creation or duplication of epitopes.
Thus, risk mutations can be identified and eliminated by this
procedure, thereby reducing the risk of making mutations that lead
to increased rather than decreased allergenicity.
Introduction of Residues for Chemical Derivatization in Epitope
Areas
[0171] In yet another embodiment, one can design the mutation, such
that amino acids suitable for chemical modification are substituted
for existing ones in the epitope areas. The protein variant can
then be conjugated to activated polymers. Which amino acids to
substitute and/or insert, depends in principle on the coupling
chemistry to be applied. The chemistry for preparation of covalent
bioconjugates can be found in "Bioconjugate Techniques", Hermanson,
G. T. (1996), Academic Press Inc., which is hereby incorporated as
reference (see below). It is preferred to make conservative
substitutions in the polypeptide when the polypeptide has to be
conjugated, as conservative substitutions secure that the impact of
the substitution on the polypeptide structure is limited. In the
case of providing additional amino groups this may be done by
substitution of arginine to lysine, both residues being positively
charged, but only the lysine having a free amino group suitable as
an attachment groups. In the case of providing additional
carboxylic acid groups the conservative substitution may for
instance be an asparagine to aspartic acid or glutamine to glutamic
acid substitution. These residues resemble each other in size and
shape, except from the carboxylic groups being present on the
acidic residues. In the case of providing SH-groups the
conservative substitution may be done by changing threonine or
serine to cysteine.
Chemical Conjugation
[0172] For chemical conjugation, the protein variant needs to be
incubate with an active or activated polymer and subsequently
separated from the unreacted polymer. This can be done in solution
followed by purification or it can conveniently be done using the
immobilized protein variants, which can easily be exposed to
different reaction environments and washes.
[0173] In the case were polymeric molecules are to be conjugated
with the polypeptide in question and the polymeric molecules are
not active they must be activated by the use of a suitable
technique. It is also contemplated according to the invention to
couple the polymeric molecules to the polypeptide through a linker.
Suitable linkers are well-known to the skilled person. Methods and
chemistry for activation of polymeric molecules as well as for
conjugation of polypeptides are intensively described in the
literature. Commonly used methods for activation of insoluble
polymers include activation of functional groups with cyanogen
bromide, periodate, glutaraldehyde, biepoxides, epichlorohydrin,
divinylsulfone, carbodiimide, sulfonyl halides, trichlorotriazine
etc. (see R. F. Taylor, (1991), "Protein immobilisation.
Fundamental and applications", Marcel Dekker, N.Y.; S. S. Wong,
(1992), "Chemistry of Protein Conjugation and Crosslinking", CRC
Press, Boca Raton; G. T. Hermanson et al., (1993), "Immobilized
Affinity Ligand Techniques", Academic Press, N.Y.). Some of the
methods concern activation of insoluble polymers but are also
applicable to activation of soluble polymers e.g. periodate,
trichlorotriazine, sulfonylhalides, divinylsulfone, carbodiimide
etc. The functional groups being amino, hydroxyl, thiol, carboxyl,
aldehyde or sulfydryl on the polymer and the chosen attachment
group on the protein must be considered in choosing the activation
and conjugation chemistry which normally consist of i) activation
of polymer, ii) conjugation, and iii) blocking of residual active
groups.
[0174] In the following a number of suitable polymer activation
methods will be described shortly. However, it is to be understood
that also other methods may be used.
[0175] Coupling polymeric molecules to the free acid groups of
polypeptides may be performed with the aid of diimide and for
example amino-PEG or hydrazino-PEG (Pollak et al., (1976), J. Am.
Chem. Soc., 98, 289-291) or diazoacetate/amide (Wong et al.,
(1992), "Chemistry of Protein Conjugation and Crosslinking", CRC
Press).
[0176] Coupling polymeric molecules to hydroxy groups is generally
very difficult as it must be performed in water. Usually hydrolysis
predominates over reaction with hydroxyl groups.
[0177] Coupling polymeric molecules to free sulfhydryl groups can
be achieved with special groups like maleimido or the ortho-pyridyl
disulfide. Also vinylsulfone (U.S. Pat. No. 5,414,135, (1995), Snow
et al.) has a preference for sulfhydryl groups but is not as
selective as the other mentioned.
[0178] Accessible arginine residues in the polypeptide chain may be
targeted by groups comprising two vicinal carbonyl groups.
[0179] Techniques involving coupling of electrophilically activated
PEGs to the amino groups of Lysines may also be useful. Many of the
usual leaving groups for alcohols give rise to an amine linkage.
For instance, alkyl sulfonates, such as tresylates (Nilsson et al.,
(1984), Methods in Enzymology vol. 104, Jacoby, W. B., Ed.,
Academic Press: Orlando, p. 56-66; Nilsson et al., (1987), Methods
in Enzymology vol. 135; Mosbach, K., Ed.; Academic Press: Orlando,
pp. 65-79; Scouten et al., (1987), Methods in Enzymology vol. 135,
Mosbach, K., Ed., Academic Press: Orlando, 1987; pp 79-84;
Crossland et al., (1971), J. Amr. Chem. Soc. 1971, 93, pp.
4217-4219), mesylates (Harris, (1985), supra; Harris et al.,
(1984), J. Polym. Sci. Polym. Chem. Ed. 22, pp 341-352), aryl
sulfonates like tosylates, and para-nitrobenzene sulfonates can be
used.
[0180] Organic sulfonyl chlorides, e.g. Tresyl chloride,
effectively converts hydroxy groups in a number of polymers, e.g.
PEG, into good leaving groups (sulfonates) that, when reacted with
nucleophiles like amino groups in polypeptides allow stable
linkages to be formed between polymer and polypeptide. In addition
to high conjugation yields, the reaction conditions are in general
mild (neutral or slightly alkaline pH, to avoid denaturation and
little or no disruption of activity), and satisfy the
non-destructive requirements to the polypeptide.
[0181] Tosylate is more reactive than the mesylate but also less
stable decomposing into PEG, dioxane, and sulfonic acid (Zalipsky,
(1995), Bioconjugate Chem., 6, 150-165). Epoxides may also been
used for creating amine bonds but are much less reactive than the
abovementioned groups.
[0182] Converting PEG into a chloroformate with phosgene gives rise
to carbamate linkages to Lysines. Essentially the same reaction can
be carried out in many variants substituting the chlorine with
N-hydroxy succinimide (U.S. Pat. No. 5,122,614, (1992); Zalipsky et
al., (1992), Biotechnol. Appl. Biochem., 15, p. 100-114; Monfardini
et al., (1995), Bioconjugate Chem., 6, 62-69, with imidazole (Allen
et al., (1991), Carbohydr. Res., 213, pp 309-319), with
para-nitrophenol, DMAP (EP 632 082 A1, (1993), Looze, Y.) etc. The
derivatives are usually made by reacting the chloroformate with the
desired leaving group. All these groups give rise to carbamate
linkages to the peptide.
[0183] Furthermore, isocyanates and isothiocyanates may be
employed, yielding ureas and thioureas, respectively.
[0184] Amides may be obtained from PEG acids using the same leaving
groups as mentioned above and cyclic imid thrones (U.S. Pat. No.
5,349,001, (1994), Greenwald et al.). The reactivity of these
compounds are very high but may make the hydrolysis to fast.
[0185] PEG succinate made from reaction with succinic anhydride can
also be used. The hereby comprised ester group make the conjugate
much more susceptible to hydrolysis (U.S. Pat. No. 5,122,614,
(1992), Zalipsky). This group may be activated with N-hydroxy
succinimide.
[0186] Furthermore, a special linker can be introduced. The most
well studied being cyanuric chloride (Abuchowski et al., (1977), J.
Biol. Chem., 252, 3578-3581; U.S. Pat. No. 4,179,337, (1979), Davis
et al.; Shafer et al., (1986), J. Polym. Sci. Polym. Chem. Ed., 24,
375-378.
[0187] Coupling of PEG to an aromatic amine followed by diazotation
yields a very reactive diazonium salt, which can be reacted with a
peptide in situ. An amide linkage may also be obtained by reacting
an azlactone derivative of PEG (U.S. Pat. No. 5,321,095, (1994),
Greenwald, R. B.) thus introducing an additional amide linkage.
[0188] As some peptides do not comprise many Lysines it may be
advantageous to attach more than one PEG to the same Lysine. This
can be done e.g. by the use of 1,3-diamino-2-propanol.
[0189] PEGs may also be attached to the amino-groups of the enzyme
with carbamate linkages (WO 95/11924, Greenwald et al.). Lysine
residues may also be used as the backbone.
[0190] The coupling technique used in the examples is the
N-succinimidyl carbonate conjugation technique descried in WO
90/13590 (Enzon).
[0191] In a preferred embodiment, the activated polymer is
methyl-PEG which has been activated by N-succinimidyl carbonate as
described WO 90/13590. The coupling can be carried out at alkaline
conditions in high yields.
[0192] For coupling of polymers to the protein variants, it is
preferred to use conditions similar to those described in WO
96/17929 and WO 99/00489 (Novo Nordisk A/S) e.g. mono or bis
activated PEG's of molecular weight ranging from 100 to 5000 Da.
For instance, a methyl-PEG 350 could be activated with
N-succinimidyl carbonate and incubated with protein variant at a
molar ratio of more than 5 calculated as equivalents of activated
PEG divided by moles of lysines in the protein of interest. For
coupling to immobilized protein variant, the PEG:protein ratio
should be optimized such that the PEG concentration is low enough
for the buffer capacity to maintain alkaline pH throughout the
reaction; while the PEG concentration is still high enough to
ensure sufficient degree of modification of the protein. Further,
it is important that the activated PEG is kept at conditions that
prevent hydrolysis (i.e. dissolved in acid or solvents) and diluted
directly into the alkaline reaction buffer. It is essential that
primary amines are not present other than those occurring in the
lysine residues of the protein. This can be secured by washing
thoroughly in borate buffer. The reaction is stopped by separating
the fluid phase containing unreacted PEG from the solid phase
containing protein and derivatized protein. Optionally, the solid
phase can then be washed with tris buffer, to block any unreacted
sites on PEG chains that might still be present.
Introduction of Consensus Sequences for Post-Translational
Modifications in the Epitope Areas
[0193] In another embodiment, the mutations are designed, such that
recognition sites for post-translational modifications are
introduced in the epitope areas, and the protein variant is
expressed in a suitable host organism capable of the corresponding
post-translational modification. These post-translational
modifications may serve to shield the epitope and hence lower the
immunogenicity of the protein variant relative to the protein
backbone. Post-translational modifications include glycosylation,
phosphorylation, N-terminal processing, acylation, ribosylation and
sulfatation. A good example is N-glycosylation. N-glycosylation is
found at sites of the sequence Asn-Xaa-Ser, Asn-Xaa-Thr, or
Asn-Xaa-Cys, in which neither the Xaa residue nor the amino acid
following the tri-peptide consensus sequence is a proline (T. E.
Creighton, `Proteins--Structures and Molecular Properties, 2nd
edition, W.H. Freeman and Co., New York, 1993, pp. 91-93). It is
thus desirable to introduce such recognition sites in the sequence
of the backbone protein. The specific nature of the glycosyl chain
of the glycosylated protein variant may be linear or branched
depending on the protein and the host cells. Another example is
phosphorylation: The protein sequence can be modified so as to
introduce serine phosphorylation sites with the recognition
sequence arg-arg-(xaa).sub.n-ser (where n=0, 1, or 2) (SEQ ID NOS:
38 and 39), which can be phosphorylated by the cAMP-dependent
kinase or tyrosine phosphorylation sites with the recognition
sequence -lys/arg-(xaa).sub.3-asp/glu-(xaa).sub.3-tyr (SEC) ID NO:
40), which can usually be phosphorylated by tyrosine-specific
kinases (T. E. Creighton, "Proteins--Structures and molecular
properties", 2nd ed., Freeman, N.Y., 1993).
Randomized Approaches to Introduce Modifications in Epitope
Areas.
[0194] In order to generate protein variants, more than one amino
acid residue may be substituted, added or deleted, these amino
acids preferably being located in different epitope areas. In that
case, it may be difficult to assess a priori how well the
functionality of the protein is maintained while antigenicity is
reduced, especially since the possible number of
mutation-combinations becomes very large, even for a small number
of mutations. In that case, it will be an advantage, to establish a
library of diversified mutants each having one or more changed
amino acids introduced and selecting those variants, which show
good retention of function and at the same time a significant
reduction in antigenicity.
[0195] A diversified library can be established by a range of
techniques known to the person skilled in the art (Reetz M T;
Jaeger K E, in `Biocatalysis--from Discovery to Application` edited
by Fessner W D, Vol. 200, pp. 31-57 (1999); Stemmer, Nature, vol.
370, p. 389-391, 1994; Zhao and Arnold, Proc. Natl. Acad. Sci.,
USA, vol. 94, pp. 7997-8000, 1997; or Yano et al., Proc. Natl.
Acad. Sci., USA, vol. 95, pp 5511-5515, 1998). These include, but
are not limited to, `spiked mutagenesis`, in which certain
positions of the protein sequence are randomized by carring out PCR
mutagenesis using one or more oligonucleotide primers which are
synthesized using a mixture of nucleotides for certain positions
(Lanio T, Jeltsch A, Biotechniques, Vol. 25(6), 958,962,964-965
(1998)). The mixtures of oligonucleotides used within each triplet
can be designed such that the corresponding amino acid of the
mutated gene product is randomized within some predetermined
distribution function. Algorithms have been disclosed, which
facilitate this design (Jensen L J et al., Nucleic Acids Research,
Vol. 26(3), 697-702 (1998)).
[0196] In an embodiment substitutions are found by a method
comprising the following steps: 1) a range of substitutions,
additions, and/or deletions are listed encompassing several epitope
areas (preferably in the corresponding epitope sequences, anchor
amino aids, and/or hot spots), 2) a library is designed which
introduces a randomized subset of these changes in the amino acid
sequence into the target gene, e.g. by spiked mutagenesis, 3) the
library is expressed, and preferred variants are selected. In
another embodiment, this method is supplemented with additional
rounds of screening and/or family shuffling of hits from the first
round of screening (J. E. Ness, et al, Nature Biotechnology, vol.
17, pp. 893-896, 1999) and/or combination with other methods of
reducing immunogenicity by genetic means (such as that disclosed in
WO 92/10755).
[0197] The library may be designed, such that at least one amino
acid of the epitope area is substituted. In a preferred embodiment
at least one amino acid of the epitope sequence itself is changed,
and in an even more preferred embodiment, one or more hot spot
amino acids are changed. The library may be biased such that
towards introducing an amino acid of different size,
hydrophilicity, and/or polarity relative to the original one of the
`protein backbone`. For example changing a small amino acid to a
large amino acid, a hydrophilic amino acid to a hydrophobic amino
acid, a polar amino acid to a non-polar amino acid or a basic to an
acidic amino acid. Other changes may be the addition or deletion of
at least one amino acid of the epitope area, preferably deleting an
anchor amino acid. Furthermore, substituting some amino acids and
deleting or adding others may change an epitope.
[0198] Diversity in the protein variant library can be generated at
the DNA triplet level, such that individual codons are variegated
e.g. by using primers of partially randomized sequence for a PCR
reaction. Further, several techniques have been described, by which
one can create a library with such diversity at several locations
in the gene, which are too far apart to be covered by a single
(spiked) oligonucleotide primer. These techniques include the use
of in vivo recombination of the individually diversified gene
segments as described in WO 97/07205 on page 3, line 8 to 29 or by
using DNA shuffling techniques to create a library of full length
genes that combine several gene segments each of which are
diversified e.g. by spiked mutagenesis (Stemmer, Nature 370, pp.
389-391, 1994 and U.S. Pat. Nos. 5,605,793 and 5,830,721). In the
latter case, one can use the gene encoding the "protein backbone"
as a template double-stranded polynucleotide and combining this
with one or more single or double-stranded oligonucleotides as
described in claim 1 of U.S. Pat. No. 5,830,721. The
single-stranded oligonucleotides could be partially randomized
during synthesis. The double-stranded oligonucleotides could be PCR
products incorporating diversity in a specific region. In both
cases, one can dilute the diversity with corresponding segments
containing the sequence of the backbone protein in order to limit
the number of changes that are on average introduced. As mentioned
above, methods have been established for designing the ratios of
nucleotides (A; C; T; G) used at a particular codon during primer
synthesis, so as to approximate a desired frequency distribution
among a set of desired amino acids at that particular codon. This
allows one to bias the partially randomized mutagenesis towards
e.g. introduction of post-translational modification sites,
chemical modification sites, or simply amino acids that are
different from those that define the epitope or the epitope area.
One could also approximate a sequence in a given location or
epitope area to the corresponding location on a homologous, human
protein.
[0199] Occasionally, one would be interested in testing a library
that combines a number of known mutations in different locations in
the primary sequence of the `protein backbone`. These could be
introduced post-translational or chemical modification sites, or
they could be mutations, which by themselves had proven beneficial
for one reason or another (e.g. decreasing antigenicity, or
improving specific activity, performance, stability, or other
characteristics). In such cases, it may be desirable to create a
library of diverse combinations of known sequences. For example if
12 individual mutations are known, one could combine (at least) 12
segments of the `protein backbone` gene in which each segment is
present in two forms: one with and one without the desired
mutation. By varying the relative amounts of those segments, one
could design a library (of size 2.sup.12) for which the average
number of mutations per gene can be predicted. This can be a useful
way of combining elements that by themselves give some, but not
sufficient effect, without resorting to very large libraries, as is
often the case when using `spiked mutagenesis`. Another way to
combine these `known mutations` could be by using family shuffling
of oligomeric DNA encoding the known changes with fragments of the
full length wild type sequence.
Assays for Reduced Allergenicity
[0200] When protein variants have been constructed based on the
methods described in this invention, it is desirable to confirm
their antibody binding capacity, functionality, immunogenicity
and/or allergenicity using a purified preparation. For that use,
the protein variant of interest can be expressed in larger scale,
purified by conventional techniques, and the antibody binding and
functionality should be examined in detail using dose-response
curves and e.g. direct or competitive ELISA (C-ELISA).
[0201] The potentially reduced allergenicity (which is likely, but
not necessarily true for a variant w. low antibody binding) should
be tested in in vivo or in vitro model systems: e.g. an in vitro
assays for immunogenicity such as assays based on cytokine
expression profiles or other proliferation or differentiation
responses of epithelial and other cells incl. B-cells and T-cells.
Further, animal models for testing allergenicity should be set up
to test a limited number of protein variants that show desired
characteristics in vitro. Useful animal models include the guinea
pig intratracheal model (GPIT) (Ritz, et al. Fund. Appl. Toxicol.,
21, pp. 31-37, 1993), mouse subcutaneous (mouse-SC) (WO 98/30682,
Novo Nordisk), the rat intratracheal (rat-IT) (WO 96/17929, Novo
Nordisk), and the mouse intranasal (MINT) (Robinson et al., Fund.
Appl. Toxicol. 34, pp. 15-24, 1996) models.
[0202] The immunogenicity of the protein variant is measured in
animal tests, wherein the animals are immunised with the protein
variant and the immune response is measured. Specifically, it is of
interest to determine the allergenicity of the protein variants by
repeatedly exposing the animals to the protein variant by the
intratracheal route and following the specific IgG and IgE titers.
Alternatively, the mouse intranasal (MINT) test can be used to
assess the allergenicity of protein variants. By the present
invention the allergenicity is reduced at least 3 times as compared
to the allergenicity of the parent protein, preferably 10 times
reduced, more preferably 50 times.
[0203] However, the present inventors have demonstrated that the
performance in ELISA correlates closely to the immunogenic
responses measured in animal tests. To obtain a useful reduction of
the allergenicity of a protein, the IgE binding capacity of the
protein variant must be reduced to at least below 75%, preferably
below 50%, more preferably below 25% of the IgE binding capacity of
the parent protein as measured by the performance in IgE ELISA,
given the value for the IgE binding capacity of the parent protein
is set to 100%.
[0204] Thus a first assessment of the immunogenicity and/or
allergenicity of a protein can be made by measuring the antibody
binding capacity or antigenicity of the protein variant using
appropriate antibodies. This approach has also been used in the
literature (WO 99/47680).
Assays for Altered Immunotherapeutic Effect
[0205] The immunotherapeutic effect of allergen vaccines can be
assessed a number of different ways. One is to measure the specific
IgE binding, the reduction of which indicates a better allergen
vaccine potential (WO 99/47680, ALK-ABELLO). Also, several cellular
assays could be employed to show the modified immuneresponse
indicative of good allergen vaccine potential as shown in several
publications, all of which are hereby incorporated by reference
(van Neerven et al, "T lymphocyte responses to allergens:
Epitope-specificity and clinical relevance", Immunol Today, 1996,
vol. 17, pp. 526-532; Hoffmann et al., Allergy, 1999, vol. 54, pp.
446-454, WO 99/07880).
[0206] Eventually, clinical trials with allergic patients could be
employed using cellular or clinical end-point measurements. (Ebner
et al., Clin. Exp. All., 1997, vol. 27, pp. 107-1015; Int. Arch.
Allergy Immunol., 1999, vol. 119, pp 1-5).
Determining Functionality
[0207] A wide variety of protein functionality assays are available
in the literature. Especially, those suitable for automated
analysis are useful for this invention. Several have been published
in the literature such as protease assays (WO 99/34011, Genencor
International; J. E. Ness, et al, Nature Biotechn., 17, pp.
893-896, 1999), oxidoreductase assays (Chemy et al., Nature
Biotechn., 17, pp. 379-384, 1999, and assays for several other
enzymes (WO 99/45143, Novo Nordisk). Those assays that employ
soluble substrates can be employed for direct analysis of
functionality of immobilized protein variants.
Cross-Reactivity
[0208] A related objective is to reduce cross-reactivity between
`commercial allergens` and `environmental allergens`.
Cross-reactivities between food allergens of different origin are
well-known (Akkerdaas et al, Allergy 50, pp 215-220, 1995).
Similarly, cross-reactivities between other environmental allergens
(like pollen, dust mites etc.) and commercial allergens (like
enzyme proteins) have been established in the literature (J. All.
Clin. Immunol., 1998, vol. 102, pp. 679-686 and by the present
inventors. The molecular reason for this cross-reactivity can be
explored using epitope mapping. By finding epitope patterns using
antibodies raised against environmental allergen (donor protein)
and mapping this information on a commercial allergen (the acceptor
protein), one may find the epitopes that are common to both
proteins, and hence responsible for the cross-reactivity.
Obviously, one can also use the commercial allergen as donor and
the environmental allergen as acceptor. By modifying the commercial
allergen using protein engineering in the epitope areas identified
as described above, one can reduce the cross-reactivity of the
commercial allergen variant towards the environmental allergens
(and vice versa). Hence, the use of the modified commercial
allergens would be safer than using the unmodified commercial
allergen.
[0209] Testing of this approach would be done using an
antibody-binding assay with the protein variant (and its parent
protein as control) and antibodies raised against the protein that
cross-reacts with the parent protein. The method is otherwise
identical to those described in the Methods section for
characterization of allergencitiy and antigenicity.
Wash Performance etc.
[0210] The modifications of the enzymes in the epitope areas as
disclosed the present application may cause other effects to the
enzyme than modified immunogenicity. A modification may also change
the performance of the enzyme, such as the wash performance, thermo
stability, storage stability and increased catalytical activity of
the enzyme.
[0211] The ability of an enzyme to catalyze the degradation of
various naturally occurring substrates present on the objects to be
cleaned during e.g. wash is often referred to as its washing
ability, wash-ability, detergency, or wash performance. Throughout
this application the term wash performance will be used to
encompass this property.
Commercial Enzyme Applications
Industrial Applications
[0212] Another aspect of the invention is a composition comprising
at least one protein (polypeptide) or enzyme of the invention. The
composition may comprise other polypeptides, proteins or enzymes
and/or ingredients normally used in personal care products, such as
shampoo, soap bars, skin lotion, skin creme, hair dye, toothpaste,
household articles, agro chemicals, personal care products, such as
cleaning preparations e.g. for contact lenses, cosmetics,
toiletries, oral and dermal pharmaceuticals, compositions used for
treating textiles, compositions used for manufacturing food, e.g.
baking, and feed etc.
[0213] Examples of said proteins(polypeptides)/enzymes include
enzymes exhibiting protease, lipolytic enzyme, oxidoreductase,
carbohydrase, transferase, such as transglutaminase, phytase and/or
anti-microbial polypeptide activity. These enzymes may be present
as conjugates with reduced activity.
[0214] The protein of the invention may furthermore typically be
used in detergent composition. It may be included in the detergent
composition in the form of a non-dusting granulate, a stabilized
liquid, or a protected enzyme. Non-dusting granulates may be
produced, e.g., as disclosed in U.S. Pat. Nos. 4,106,991 and
4,661,452 (both to Novo Industri NS) and may optionally be coated
by methods known in the art. Examples of waxy coating materials are
poly(ethylene oxide) products (polyethylene glycol, PEG) with mean
molecular weights of 1000 to 20000; ethoxylated nonylphenols having
from 16 to 50 ethylene oxide units; ethoxylated fatty alcohols in
which the alcohol contains from 12 to 20 carbon atoms and in which
there are 15 to 80 ethylene oxide units; fatty alcohols; fatty
acids; and mono- and di- and triglycerides of fatty acids. Examples
of film-forming coating materials suitable for application by fluid
bed techniques are given in patent GB 1483591. Liquid enzyme
preparations may, for instance, be stabilized by adding a polyol
such as propylene glycol, a sugar or sugar alcohol, lactic acid or
boric acid according to established methods. Other enzyme
stabilizers are well known in the art. Protected enzymes may be
prepared according to the method disclosed in EP 238,216.
[0215] The detergent composition may be in any convenient form,
e.g. as powder, granules, paste or liquid. A liquid detergent may
be aqueous, typically containing up to 70% water and 0-30% organic
solvent, or non-aqueous.
[0216] The detergent composition comprises one or more surfactants,
each of which may be anionic, nonionic, cationic, or zwitterionic.
The detergent will usually contain 0-50% of anionic surfactant such
as linear alkylbenzenesulfonate (LAS), alpha-olefinsulfonate (AOS),
alkyl sulfate (fatty alcohol sulfate) (AS), alcohol ethoxysulfate
(AEOS or AES), secondary alkanesulfonates (SAS), alpha-sulfo fatty
acid methyl esters, alkyl- or alkenylsuccinic acid, or soap. It may
also contain 0-40% of nonionic surfactant such as alcohol
ethoxylate (AEO or AE), carboxylated alcohol ethoxylates,
nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine
oxide, ethoxylated fatty acid monoethanolamide, fatty acid
monoethanolamide, or polyhydroxy alkyl fatty acid amide (e.g. as
described in WO 92/06154).
[0217] The detergent composition may additionally comprise one or
more other enzymes, such as e.g. proteases, amylases, lipolytic
enzymes, cutinases, cellulases, peroxidases, oxidases, and further
anti-microbial polypeptides.
[0218] The detergent may contain 1-65% of a detergent builder or
complexing agent such as zeolite, diphosphate, triphosphate,
phosphonate, citrate, nitrilotriacetic acid (NTA),
ethylenediaminetetraacetic acid (EDTA),
diethylenetriaminepentaacetic acid (DTMPA), alkyl- or
alkenylsuccinic acid, soluble silicates or layered silicates (e.g.
SKS-6 from Hoechst). The detergent may also be unbuilt, i.e.
essentially free of detergent builder.
[0219] The detergent may comprise one or more polymers. Examples
are carboxymethylcellulose (CMC), poly(vinylpyrrolidone) (PVP),
polyethyleneglycol (PEG), poly(vinyl alcohol) (PVA),
polycarboxylates such as polyacrylates, maleic/acrylic acid
copolymers and lauryl methacrylate/acrylic acid copolymers.
[0220] The detergent may contain a bleaching system which may
comprise a H.sub.2O.sub.2 source such as perborate or percarbonate
which may be combined with a peracid-forming bleach activator such
as tetraacetylethylenediamine (TAED) or
nonanoyloxybenzenesulfon-ate (NOBS). Alternatively, the bleaching
system may comprise peroxyacids of, e.g., the amide, imide, or
sulfone type.
[0221] The detergent composition of the invention comprising the
polypeptide of the invention may be stabilized using conventional
stabilizing agents, e.g. a polyol such as propylene glycol or
glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a
boric acid derivative such as, e.g., an aromatic borate ester, and
the composition may be formulated as described in, e.g., WO
92/19709 and WO 92/19708.
[0222] The detergent may also contain other conventional detergent
ingredients such as, e.g., fabric conditioners including clays,
foam boosters, suds suppressors, anti-corrosion agents,
soil-suspending agents, anti-soil-redeposition agents, dyes,
bactericides, optical brighteners, or perfume.
[0223] The pH (measured in aqueous solution at use concentration)
will usually be neutral or alkaline, e.g. in the range of 7-11.
Dishwashing Composition
[0224] Further, a modified enzyme according to the invention may
also be used in dishwashing detergents.
[0225] Dishwashing detergent compositions comprise a surfactant
which may be anionic, non-ionic, cationic, amphoteric or a mixture
of these types. The detergent will contain 0-90% of non-ionic
surfactant such as low- to non-foaming ethoxylated propoxylated
straight-chain alcohols.
[0226] The detergent composition may contain detergent builder
salts of inorganic and/or organic types. The detergent builders may
be subdivided into phosphorus-containing and
non-phosphorus-containing types. The detergent composition usually
contains 1-90% of detergent builders.
[0227] Examples of phosphorus-containing inorganic alkaline
detergent builders, when present, include the water-soluble salts
especially alkali metal pyrophosphates, orthophosphates, and
polyphosphates. An example of phosphorus-containing organic
alkaline detergent builder, when present, includes the
water-soluble salts of phosphonates. Examples of
non-phosphorus-containing inorganic builders, when present, include
water-soluble alkali metal carbonates, borates and silicates as
well as the various types of water-insoluble crystalline or
amorphous alumino silicates of which zeolites are the best-known
representatives.
[0228] Examples of suitable organic builders include the alkali
metal, ammonium and substituted ammonium, citrates, succinates,
malonates, fatty acid sulphonates, carboxymetoxy succinates,
ammonium polyacetates, carboxylates, polycarboxylates,
aminopolycarboxylates, polyacetyl carboxylates and
polyhydroxsulphonates.
[0229] Other suitable organic builders include the higher molecular
weight polymers and co-polymers known to have builder properties,
for example appropriate polyacrylic acid, polymaleic and
polyacrylic/polymaleic acid copolymers and their salts.
[0230] The dishwashing detergent composition may contain bleaching
agents of the chlorine/bromine-type or the oxygen-type. Examples of
inorganic chlorine/bromine-type bleaches are lithium, sodium or
calcium hypochlorite and hypobromite as well as chlorinated
trisodium phosphate. Examples of organic chlorine/bromine-type
bleaches are heterocyclic N-bromo and N-chloro imides such as
trichloroisocyanuric, tribromoisocyanuric, dibromoisocyanuric and
dichloroisocyanuric acids, and salts thereof with
water-solubilizing cations such as potassium and sodium. Hydantoin
compounds are also suitable.
[0231] The oxygen bleaches are preferred, for example in the form
of an inorganic persalt, preferably with a bleach precursor or as a
peroxy acid compound. Typical examples of suitable peroxy bleach
compounds are alkali metal perborates, both tetrahydrates and
monohydrates, alkali metal percarbonates, persilicates and
perphosphates. Preferred activator materials are TAED and glycerol
triacetate.
[0232] The dishwashing detergent composition of the invention may
be stabilized using conventional stabilizing agents for the
enzyme(s), e.g. a polyol such as e.g. propylene glycol, a sugar or
a sugar alcohol, lactic acid, boric acid, or a boric acid
derivative, e.g. an aromatic borate ester.
[0233] The dishwashing detergent composition of the invention may
also contain other conventional detergent ingredients, e.g.
deflocculant material, filler material, foam depressors,
anti-corrosion agents, soil-suspending agents, sequestering agents,
anti-soil redeposition agents, dehydrating agents, dyes,
bactericides, fluorescers, thickeners and perfumes.
[0234] Finally, the enzyme of the invention may be used in
conventional dishwashing-detergents, e.g. in any of the detergents
described in any of the following patent publications: EP 518719,
EP 518720, EP 518721, EP 516553, EP 516554, EP 516555, GB 2200132,
DE 3741617, DE 3727911, DE 4212166, DE 4137470, DE 3833047, WO
93/17089, DE 4205071, WO 52/09680, WO 93/18129, WO 93/04153, WO
92/06157, WO 92/08777, EP 429124, WO 93/21299, U.S. Pat. No.
5,141,664, EP 561452, EP 561446, GB 2234980, WO 93/03129, EP
481547, EP 530870, EP 533239, EP 554943, EP 346137, U.S. Pat. No.
5,112,518, EP 318204, EP 318279, EP 271155, EP 271156, EP 346136,
GB 2228945, CA 2006687, WO 93/25651, EP 530635, EP 414197, and U.S.
Pat. No. 5,240,632.
Personal Care Applications
[0235] A particularly useful application area for low allergenic
proteins or of proteins with low cross-reactivity to environmental
allergens would be in personal care products where the end-user is
in close contact with the protein, and where certain problems with
allergenicity has been encountered in experimental set-ups (Kelling
et al., J. All. Clin. 1 mm., 1998, Vol. 101, pp. 179-187 and
Johnston et al., Hum. Exp. Toxicol., 1999, Vol. 18, p. 527).
[0236] First of all the conjugate or compositions of the invention
can advantageously be used for personal care products, such as hair
care and hair treatment products. This include products such as
shampoo, balsam, hair conditioners, hair waving compositions, hair
dyeing compositions, hair tonic, hair liquid, hair cream, shampoo,
hair rinse, hair spray.
[0237] Further contemplated are oral care products such as
dentifrice, oral washes, chewing gum.
[0238] Also contemplated are skin care products and cosmetics, such
as skin cream, skin milk, cleansing cream, cleansing lotion,
cleansing milk, cold cream, cream soap, nourishing essence, skin
lotion, milky lotion, calamine lotion, hand cream, powder soap,
transparent soap, sun oil, sun screen, shaving foam, shaving cream,
baby oil lipstick, lip cream, creamy foundation, face powder,
powder eye-shadow, powder, foundation, make-up base, essence
powder, whitening powder.
[0239] Also for contact lenses hygiene products the conjugate of
the invention can be used advantageously. Such products include
cleaning and disinfection products for contact lenses.
Proteases
[0240] Proteases are well-known active ingredients for cleaning of
contact lenses. They hydrolyse the proteinaceous soil on the lens
and thereby makes it soluble. Removal of the protein soil is
essential for the wearing comfort.
[0241] Proteases are also effective ingredients in skin cleaning
products, where they remove the upper layer of dead keratinaseous
skin cells and thereby make the skin look brighter and fresher.
[0242] Proteases are also used in oral care products, especially
for cleaning of dentures, but also in dentifrices.
[0243] Further, proteases are used in toiletries, bath and shower
products, including shampoos, conditioners, lotions, creams, soap
bars, toilet soaps, and liquid soaps.
Lipolytic Enzymes
[0244] Lipolytic enzymes can be applied for cosmetic use as active
ingredients in skin cleaning products and anti-acne products for
removal of excessive skin lipids, and in bath and shower products
such as creams and lotions as active ingredients for skin care.
[0245] Lipolytic enzymes can also be used in hair cleaning products
(e.g. shampoos) for effective removal of sebum and other fatty
material from the surface of hair.
[0246] Lipolytic enzymes are also effective ingredients in products
for cleaning of contact lenses, where they remove lipid deposits
from the lens surface.
Oxidoreductases
[0247] The most common oxidoreductase for personal care purposes is
an oxidase (usually glucose oxidase) with substrate (e.g. glucose)
that ensures production of H.sub.2O.sub.2, which then will initiate
the oxidation of for instance SON.sup.- or I.sup.- into
antimicrobial reagents (SCNO.sup.- or I.sub.2) by a peroxidase
(usually lactoperoxidase). This enzymatic complex is known in
nature from e.g. milk and saliva.
[0248] It is being utilised commercially as anti-microbial system
in oral care products (mouth rinse, dentifrice, chewing gum) where
it also can be combined with an amyloglucosidase to produce the
glucose. These systems are also known in cosmetic products for
preservation.
[0249] Anti-microbial systems comprising the combination of an
oxidase and a peroxidase are know in the cleaning of contact
lenses.
[0250] Another application of oxidoreductases is oxidative hair
dyeing using oxidases, peroxidases and laccases.
[0251] Free radicals formed on the surface of the skin (and hair)
known to be associated with the ageing process of the skin
(spoilage of the hair). The free radicals activate chain reactions
that lead to destruction of fatty membranes, collagen, and cells.
The application of free radical scavengers such as Superoxide
dismutase into cosmetics is well known (R. L. Goldemberg, DCI, Nov.
93, p. 48-52).
[0252] Protein disulfide isomerase (PDI) is also an oxidoreductase.
It can be utilised for waving of hair (reduction and reoxidation of
disulfide bonds in hair) and repair of spoiled hair (where the
damage is mainly reduction of existing disulfide bonds).
Carbohydrases
[0253] Plaque formed on the surface of teeth is composed mainly of
polysaccharides. They stick to the surface of the teeth and the
microorganisms. The polysaccharides are mainly .alpha.-1,6 bound
glucose (dextran) and .alpha.-1,3 bound glucose (mutan). The
application of different types of glucanases such as mutanase and
dextranase helps hydrolysing the sticky matrix of plaque, making it
easier to remove by mechanical action.
[0254] Also other kinds of biofilm for instance the biofilm formed
in lens cases can be removed by the action of glucanases.
Food and Feed
[0255] Further conjugated enzymes or polypeptides with reduced
immunogenicity according to the invention may advantageously be
used in the manufacturing of food and feed.
Proteases
[0256] The gluten in wheat flour is the essential ingredient
responsible for the ability of flour to be used in baked
foodstuffs. Proteolytic enzymes are sometimes needed to modify the
gluten phase of the dough, e.g. a hard wheat flour can be softened
with a protease.
[0257] Neutrase.RTM. is a commercially available neutral metallo
protease that can be used to ensure a uniform dough quality and
bread texture, and to improve flavour. The gluten proteins are
degraded either moderately or more extensively to peptides, whereby
close control is necessary in order to avoid excessive softening of
the dough.
[0258] Proteases are also used for modifying milk protein.
[0259] To coagulate casein in milk when producing cheese proteases
such as rennet or chymosin may be used.
[0260] In the brewery industry proteases are used for brewing with
unmalted cereals and for controlling the nitrogen content.
[0261] In animal feed products proteases are used so to speak to
expand the animals digestion system.
Lipolytic Enzymes
[0262] Addition of lipolytic enzyme results in improved dough
properties and an improved breadmaking quality in terms of larger
volume, improved crumb structure and whiter crumb colour. The
observed effect can be explained by a mechanism where the lipolytic
enzyme changes the interaction between gluten and some lipids
fragment during dough mixing. This results in an improved gluten
network.
[0263] The flavour development of blue roan cheese (e.g. Danablue),
certain Italian type cheese, and other dairy products containing
butter-fat, are dependent on the degradation of milk fat into free
fatty acids. Lipolytic enzymes may be used for developing flavour
in such products.
[0264] In the oil- and fat producing industry lipases are used e.g.
to minimize the amount of undesirable side-products, to modify fats
by interesterification, and to synthesis of esters.
Oxidoreductases
[0265] Further oxidoreductases with reduced immunogenicity
according to the invention may advantageously be used in the
manufacturing of food and feed.
[0266] Several oxidoreductases are used for baking, glucose
oxidase, lipoxygenase, peroxidase, catalase and combinations
hereof. Traditionally, bakers strengthen gluten by adding ascorbic
acid and potassium bromate. Some oxidoreductases can be used to
replace bromate in dough systems by oxidation of free sulfydryl
units in gluten proteins. Hereby disulphide linkages are formed
resulting in stronger, more elastic doughs with greater
resistance.
[0267] Gluzyme.TM. (Novozymes A/S) is a glucose oxidase preparation
with catalase activity that can be used to replace bromate. The
dough strengthen is measured as greater resistance to mechanical
shock, better oven spring and larger loaf volume.
Carbohydrases
[0268] Flour has varying content of amylases leading to differences
in the baking quality. Addition of amylases can be necessary in
order to standardize the flour. Amylases and pentosanases generally
provide sugar for the yeast fermentation, improve the bread volume,
retard retrogradation, and decrease the staling rate and stickiness
that results from pentosan gums. Examples of carbohydrases are
given below.
[0269] Certain maltogenic amylases can be used for prolonging the
shelf life of bread for two or more days without causing gumminess
in the product. Selectively modifies the gelatinized starch by
cleaving from the non-reducing end of the starch molecules, low
molecular wight sugars and dextrins. The starch is modified in such
a way that retrogradation is less likely to occur. The produced
low-molecular-weight sugars improve the baked goods water retention
capacity without creating the intermediate-length dextrins that
result in gumminess in the finished product. The enzyme is
inactivated during bread baking, so it can be considered a
processing aid that does not have to be declared on the label.
Overdosing of Novamyl can almost be excluded.
[0270] The bread volume can be improved by fungal .alpha.-amylases
which further provide good and uniform structure of the bread
crumb. Said .alpha.-amylases are endoenzymes that produce maltose,
dextrins and glucose. Cereal and some bacterial .alpha.-amylases
are inactivated at temperatures above the gelatinization
temperature of starch, therefore when added to wheat dough it
results in a low bread volume and a sticky bread interior. Fungamyl
has the advantage of being thermolabile and is inactivated just
below the gelatinization temperature.
[0271] Enzyme preparations containing a number of pentosanase and
hemi-cellulase activities can improve the handling and stability of
the dough, and improves the freshness, the crumb structure and the
volume of the bread.
[0272] By hydrolysing the pentosans fraction in flour, it will lose
a great deal of its water-binding capacity, and the water will then
be available for starch and gluten. The gluten becomes more pliable
and extensible, and the starch gelatinizes more easily.
Pentosanases can be used in combination with or as an alternative
to emulsifiers.
[0273] Further carbohydrases are user for producing syrups from
starch, which are widely used in soft drinks, sweets, meat
products, dairy products, bread products, ice cream, baby food, jam
etc.
[0274] The conversion of starch is normally carried out three
steps. First the starch is liquefied, by the use of alpha-amylases.
Maltodextrins, primary consisting of oligosaccharides and dextrins,
are obtained.
[0275] The mixture is then treated with an amyloglucosidase for
hydrolysing the oligosaccharides and dextrins into glucose. This
way a sweeter product is obtained. If high maltose syrups are
desired beta-amylases alone or in combination with a pullulanase
(de-branching enzyme) may be used.
[0276] The glucose mixture can be made even sweeter by
isomerization to fructose. For this an immobilized glucose
isomerase can be used.
[0277] In the sugar industry, it is common practice to speed up the
break down of present starch in cane juices. Thereby the starch
content in the raw sugar is reduced and filtration at the refinery
facilitated.
[0278] Furthermore dextranases are used to break down dextran in
raw sugar juices and syrups.
[0279] In the alcohol industry alpha-amylases is advantageously
being used for thinning of starch in distilling mashes.
[0280] In the brewing industry alpha-amylases is used for adjunct
liquefaction.
[0281] In the dairy industry beta-galactosidases (lactase) is used
when producing low lactose milk for persons suffering from lactose
malabsorption.
[0282] When flavoured milk drinks are produced from lactase-treated
milk, the addition of sugar can be reduced without reducing the
sweetness of the product.
[0283] In the production of condensed milk, lactose crystallization
can be avoided by lactase treatment, and the risk of thickening
caused by casein coagulation in lactose crystals is thus
reduced.
[0284] When producing ice cream made from lactase-treated milk (or
whey) no lactose crystals will be formed and the defect, sandiness,
will not occur.
[0285] Further, xylanases are known to be used within a number of
food/feed industrial applications as described in WO 94/21785 (Novo
Nordisk A/S).
[0286] Alpha-amylases are used in the animal feed industry to be
added to cereal-containing feed to improve the digestibility of
starch.
Anti-Microbial Polypeptides
[0287] Certain bacteriolytic enzymes may be used e.g. to wash
carcasses in the meat packing industry (see U.S. Pat. No. 5,354,681
from Novo Industri A/S)
Transferases
[0288] Transglutaminases with reduced immunogenicity according to
the invention may advantageously be used in the manufacturing of
food and feed.
[0289] Transglutaminases has the ability to crosslinking
protein.
[0290] This property can be used for gelling of aqueous phases
containing proteins. This may be used for when producing of spreads
(DK patent application no. 1071/84 from Novo Nordisk A/S).
[0291] Transglutaminases are being used for improvement of baking
quality of flour e.g. by modifying wheat flour to be used in the
preparation of cakes with improved properties, such as improved
taste, dent, mouth-feel and a higher volume (see JP 1-110147).
[0292] Further producing paste type food material e.g. used as fat
substitution in foods as ice cream, toppings, frozen desserts,
mayonnaises and low fat spreads (see WO 93/22930 from Novo Nordisk
A/S).
[0293] Furthermore for preparation of gels for yoghurt, mousses,
cheese, puddings, orange juice, from milk and milk-like products,
and binding of chopped meat product, improvement of taste and
texture of food proteins (see WO 94/21120 and WO 94/21129 from Novo
Nordisk A/S).
Phytases
[0294] Phytases of the invention may advantageously be used in the
manufacturing of food, such as breakfast cereal, cake, sweets,
drinks, bread or soup etc., and animal feed.
[0295] Phytases may be used either for exploiting the phosphorus
bound in the phytate/phytic acid present in vegetable protein
sources or for exploiting the nutritionally important minerals
bound in phytic acid complexes.
[0296] Microbial phytase may be added to feedstuff of monogastric
animals in order to avoid supplementing the feed with inorganic
phosphorus (see U.S. Pat. No. 3,297,548).
[0297] Further phytases may be used in soy processing. Soyabean
meal may contain high levels of the anti-nutritional factor phytate
which renders this protein source unsuitable for application in
baby food and feed for fish, calves and other non-ruminants, since
the phytate chelates essential minerals present therein (see EP 0
420 358).
[0298] Also for baking purposes phytases may be used. Bread with
better quality can be prepared by baking divided pieces of a dough
containing wheat flour etc. and phytase (see JP-0-3076529-A).
[0299] A high phytase activity as in koji mold are known to be used
for producing refined sake (see JP-0-6070749-A).
Textile Applications
Proteases
[0300] Proteases are used for degumming and sand washing of
silk.
Lipolytic Enzymes
[0301] Lipolytic enzymes are used for removing fatty matter
containing hydrophobic esters (e.g. triglycerides) during the
finishing of textiles (see e.g. WO 93/13256 from Novo Nordisk
A/S).
Oxidoreductases
[0302] In bleach clean up of textiles catalases may serve to remove
excess hydrogen peroxide.
Carbohydrases
[0303] Cellulolytic enzymes are widely used in the finishing of
denim garments in order to provide a localized variation in the
colour density of the fabric (Enzyme facilitated "stone wash").
[0304] Also cellulolytic enzymes find use in the bio-polishing
process. Bio-Polishing is a specific treatment of the yarn surface
which improves fabric quality with respect to handle and appearance
without loss of fabric wettability. Bio-polishing may be obtained
by applying the method described e.g. in WO 93/20278.
[0305] During the weaving of textiles, the threads are exposed to
considerable mechanical strain. In order to prevent breaking, the
threads are usually reinforced by the coating (sizing) with a
gelatinous substance (size). The most common sizing agent is starch
in native or modified form. A uniform and durable finish can thus
be obtained only after removal of the size from the fabric, the
so-called desizing. Desizing of fabrics sized with a size
containing starch or modified starch is preferably facilitated by
use of amylolytic enzymes.
Oral and Dermal Pharmaceuticals
Proteases
[0306] Different combinations of highly purified proteases (e.g.
Trypsin and Chymotrypsin) are used in pharmaceuticals to be taken
orally, and dermal pharmaceuticals for combating e.g.
inflammations, edemata and injuries.
Leather Production
Transferase
[0307] Transglutaminase is known to be used to casein-finishing
leather by acting as a hardening agent (see WO 94/13839 from Novo
Nordisk).
Hard Surface Cleaning
[0308] Cleaning of hard surfaces e.g. in the food industry is often
difficult, as equipment used for producing dairies, meat, sea food
products, beverages etc. often have a complicated shape. The use of
surfactant compositions in the form gels and foams comprising
enzymes have shown to facilitate and improve hard surface cleaning.
Enzymes, which advantageously may be added in such surfactant
compositions, are in particular proteases, lipolytic enzymes,
amylases and cellulases.
[0309] Such hard surface cleaning compositions comprising enzymes
may also advantageously be used in the transport sector, for
instance for washing cars and for general vessel wash.
[0310] Furthermore this invention relates to the method by which
the protein variants are being synthesised and expressed in host
cells. This is achieved by culturing host cells capable of
expressing a polypeptide in a suitable culture medium to obtain
expression and secretion of the polypeptide into the medium,
followed by isolation of the polypeptide from the culture medium.
The host cell may be any cell suitable for the large-scale
production of proteins, capable of expressing a protein and being
transformed by an expression vector.
[0311] The host cell comprises a DNA construct as defined above,
optionally the cells may be transformed with an expression vector
comprising a DNA construct as defined above. The host cell is
selected from any suitable cell, such as a bacterial cell, a fungal
cell, an animal cell, such as an insect cell or a mammalian cell,
or a plant cell.
Immunotherapy
[0312] A number of vaccination approaches have been described to
for infective diseases as well as for non-infective diseases (such
as cancers). In a number of cases, the antigen provided is an
isolated protein or protein-adjuvant mixture and more and more
often, the protein is recombinant (e.g. the hepatitits B vaccine
from Merck & Co). In these cases, it could be desirable to
modify the immunogenicity of the antigen vaccine, such that it
offers a stronger or more specific protection. This can be achieved
by protein engineering of the amino acid sequence of the antigen,
and would be greatly facilitated by the use of the methods of this
invention for identification of epitopes on the antigen vaccine to
be the favored sites for modification.
[0313] There are several examples of vaccine molecules that have
been engineered to achieve a specific immune protection against
virus, parasites or cancer (Ryu and Nam, Biotechnol. Prog., 2000,
vol. 16 pp. 2-16; and references cited therein). "The goal is often
to vaccinate with a minimal strucutre consisting of a well-defined
antigen, to stimulate an effective specific immune response, while
avoiding potentially hazardous risks" (Ryu and Nam, Biotechnol.
Prog., 2000, vol. 16 pp. 2-16). Thus, the methods of this invention
can be used to identify such minimal structures that define an
antigen (or epitope thereof) whether in the form of the parent
protein scaffold with a number of mutations introduced in it, or
whether it is in the form of the antibody binding peptides
themselves.
Allergen Vaccines
[0314] Today, a patient suffering allergic disease may be subjected
to allergy vaccine therapy using allergens selected on the basis of
testing the specificity of the patient's serum IgE against a bank
of allergen extracts (or similar specificity tests of the patient's
sensibilization such as skin prick test.
[0315] One could improve the quality of characterization by using
antibody binding peptides corresponding to various epitope
sequences on the protein allergens of interest. This would require
a kit comprising reagents for such specificity characterization,
e.g. the antibody binding peptides of desired specificity. It would
be preferred to use antibody binding sequences in the kit, which
correspond to defined epitope sequences known to be specific for
the allergen under investigation (i.e. not identified on other
allergens and/or not cross-reacting with sera raised against other
allergens). This kit would be useful to specifying which allergy
the patient is suffering from. This kit will lead to a more
specific answer than those kits used today, and hence to a better
selection of allergen vaccine therapy for the individual
patient.
[0316] Further, the knowledge about cross-reacting epitopes may
improve vaccine development.
[0317] In an extension of this approach, one could also
characterize the patient's serum by identifying the corresponding
antibody binding peptides among a random display library using the
aforementioned methods. This again may lead to a better selection
of allergen vaccine therapy.
[0318] Further, one could use the individual antibody binding
sequences as allergen vaccines leading to more specific allergen
vaccine. These antibody binding sequences could be administered in
an isolated form or fused to a membrane protein of the phage
display system, or to another protein, which may have beneficial
effect for the immunoprotective effect of the antibody binding
peptide (Dalum et al., Nature Biotechnology, 1999, Vol. 17, pp.
666-669).
D) Variations Possible
Parent Protein
[0319] The "parent protein" can in principle be any protein
molecule of biological origin, non-limiting examples of which are
peptides, polypeptides, proteins, enzymes, post-translationally
modified polypeptides such as lipopeptides or glycosylated
peptides, anti-microbial peptides or molecules, and proteins having
pharmaceutical properties etc.
[0320] Accordingly the invention relates to a method, wherein the
"parent protein" is chosen from the group consisting of
polypeptides, small peptides, lipopeptides, antimicrobials, and
pharmaceutical polypeptides.
[0321] The term "pharmaceutical polypeptides" is defined as
polypeptides, including peptides, such as peptide hormones,
proteins and/or enzymes, being physiologically active when
introduced into the circulatory system of the body of humans and/or
animals.
[0322] Pharmaceutical polypeptides are potentially immunogenic as
they are introduced into the circulatory system.
[0323] Examples of "pharmaceutical polypeptides" contemplated
according to the invention include insulin, ACTH, glucagon,
somatostatin, somatotropin, thymosin, parathyroid hormone,
pigmentary hormones, somatomedin, erythropoietin, luteinizing
hormone, chorionic gonadotropin, hypothalmic releasing factors,
antidiuretic hormones, thyroid stimulating hormone, relaxin,
interferon, thrombopoietin (TPO) and prolactin.
[0324] However, the proteins are preferably to be used in industry,
housekeeping and/or medicine, such as proteins used in personal
care products (for example shampoo; soap; skin, hand and face
lotions; skin, hand and face cremes; hair dyes; toothpaste), food
(for example in the baking industry), detergents and
pharmaceuticals.
Antimicrobial Peptides.
[0325] The antimicrobial peptide (AMP) may be, e.g., a
membrane-active antimicrobial peptide, or an antimicrobial peptide
affecting/interacting with intracellular targets, e.g. binding to
cell DNA. The AMP is generally a relatively short peptide,
consisting of less than 100 amino acid residues, typically 20-80
residues. The antimicrobial peptide has bactericidal and/or
fungicidal effect, and it may also have antiviral or antitumour
effects. It generally has low cytotoxicity against normal mammalian
cells.
[0326] The antimicrobial peptide is generally highly cationic and
hydrophobic. It typically contains several arginine and lysine
residues, and it may not contain a single glutamate or asparatate.
It usually contains a large proportion of hydrophobic residues. The
peptide generally has an amphiphilic structure, with one surface
being highly positive and the other hydrophobic.
[0327] The bioactive peptide and the encoding nucleotide sequence
may be derived from plants, invertebrates, insects, amphibians and
mammals, or from microorganisms such as bacteria and fungi.
[0328] The antimicrobial peptide may act on cell membranes of
target microorganisms, e.g. through nonspecific binding to the
membrane, usually in a membrane-parallel orientation, interacting
only with one face of the bilayer.
[0329] The antimicrobial peptide typically has a structure
belonging to one of five major classes: a helical, cystine-rich
(defensin-like), b-sheet, peptides with an unusual composition of
regular amino acids, and peptides containing uncommon modified
amino acids.
[0330] Examples of alpha-helical peptides are Magainin 1 and 2;
Cecropin A, B and P1; CAP18; Andropin; Clavanin A or AK; Styelin D
and C; and Buforin II. Examples of cystine-rich peptides are
a-Defensin HNP-1 (human neutrophil peptide) HNP-2 and HNP-3;
b-Defensin-12, Drosomycin, g1-purothionin, and Insect defensin A.
Examples of b-sheet peptides are Lactoferricin B, Tachyplesin I,
and Protegrin PG1-5. Examples of peptides with an unusual
composition are Indolicidin; PR-39; Bactenicin Bac5 and Bac7; and
Histatin 5. Examples of peptides with unusual amino acids are
Nisin, Gramicidin A, and Alamethicin.
[0331] Another example is the antifungal peptide (AFP) from
Aspergillus giganteus. As explained in detail in WO 94/01459, which
is hereby incorporated by reference, the antifungal polypeptide
having the amino acid sequence shown in FIG. 1 has been found in
several strains of the fungal species A. giganteus, an example of
which is the A. giganteus strain deposited with the Centraallbureau
voor Schimmelcultures (CBS) under the deposition number CBS
526.65.
[0332] However, the antifungal polypeptide, or variants thereof,
suitable for the use according to the invention are expected to be
derivable from other fungal species, especially other Aspergillus
species such as A. pallidus, A. clavatus, A. longivesica, A.
rhizopodus and A. clavatonanicus, because of the close relationship
which exists between these species and A. giganteus.
[0333] In one embodiment of the invention the protein is an enzyme,
such as glycosyl hydrolases, carbohydrases, peroxidases, proteases,
lipolytic enzymes, phytases, polysaccharide lyases,
oxidoreductases, transglutaminases and glycoseisomerases, in
particular the following.
Parent Proteases
[0334] Parent proteases (i.e. enzymes classified under the Enzyme
Classification number E.C. 3.4 in accordance with the
Recommendations (1992) of the International Union of Biochemistry
and Molecular Biology (IUBMB)) include proteases within this
group.
[0335] Examples include proteases selected from those classified
under the Enzyme Classification (E.C.) numbers:
[0336] 3.4.11 (i.e. so-called aminopeptidases), including 3.4.11.5
(Prolyl aminopeptidase), 3.4.11.9 (X-pro aminopeptidase), 3.4.11.10
(Bacterial leucyl aminopeptidase), 3.4.11.12 (Thermophilic
aminopeptidase), 3.4.11.15 (Lysyl aminopeptidase), 3.4.11.17
(Tryptophanyl aminopeptidase), 3.4.11.18 (Methionyl
aminopeptidase).
[0337] 3.4.21 (i.e. so-called serine endopeptidases), including
3.4.21.1 (Chymotrypsin), 3.4.21.4 (Trypsin), 3.4.21.25 (Cucumisin),
3.4.21.32 (Brachyurin), 3.4.21.48 (Cerevisin) and 3.4.21.62
(Subtilisin);
[0338] 3.4.22 (i.e. so-called cysteine endopeptidases), including
3.4.22.2 (Papain), 3.4.22.3 (Ficain), 3.4.22.6 (Chymopapain),
3.4.22.7 (Asclepain), 3.4.22.14 (Actimidain), 3.4.22.30 (Caricain)
and 3.4.22.31 (Ananain);
[0339] 3.4.23 (i.e. so-called aspartic endopeptidases), including
3.4.23.1 (Pepsin A), 3.4.23.18 (Aspergillopepsin I), 3.4.23.20
(Penicillopepsin) and 3.4.23.25 (Saccharopepsin); and
[0340] 3.4.24 (i.e. so-called metalloendopeptidases), including
3.4.24.28 (Bacillolysin).
Serine Proteases
[0341] A serine protease is an enzyme which catalyzes the
hydrolysis of peptide bonds, and in which there is an essential
serine residue at the active site (White, Handler and Smith, 1973
"Principles of Biochemistry," Fifth Edition, McGraw-Hill Book
Company, NY, pp. 271-272).
[0342] The bacterial serine proteases have molecular weights in the
20,000 to 45,000 Dalton range. They are inhibited by
diisopropylfluorophosphate. They hydrolyze simple terminal esters
and are similar in activity to eukaryotic chymotrypsin, also a
serine protease. A more narrow term, alkaline protease, covering a
sub-group, reflects the high pH optimum of some of the serine
proteases, from pH 9.0 to 11.0 (for review, see Priest (1977)
Bacteriological Rev. 41 711-753).
Subtilases
[0343] A sub-group of the serine proteases tentatively designated
subtilases has been proposed by Siezen et al., Protein Engng. 4
(1991) 719-737 and Siezen et al. Protein Science 6 (1997) 501-523.
They are defined by homology analysis of more than 170 amino acid
sequences of serine proteases previously referred to as
subtilisin-like proteases. A subtilisin was previously often
defined as a serine protease produced by Gram-positive bacteria or
fungi, and according to Siezen et al. now is a subgroup of the
subtilases. A wide variety of subtilases have been identified, and
the amino acid sequence of a number of subtilases has been
determined. For a more detailed description of such subtilases and
their amino acid sequences reference is made to Siezen et al.,
(1997).
Savinase-Like Subtilisin
[0344] One subgroup of the subtilases may be classified as
savinase-like subtilisins, having at least 81% homology to
Savinase, preferably at least 85% homology, more preferably at
least 90% homology, even more preferably at least 96% homology,
most preferably at least 98% homology to Savinase.
Parent Subtilase
[0345] The term "parent subtilase" describes a subtilase defined
according to Siezen et al. (1991 and 1997). For further details see
description of "SUBTILASES" immediately above. A parent subtilase
may also be a subtilase isolated from a natural source, wherein
subsequent modifications have been made while retaining the
characteristic of a subtilase. Furthermore, a parent subtilase may
also be a subtilase which has been prepared by the DNA shuffling
technique, such as described by J. E. Ness et al., Nature
Biotechnology, 17, 893-896 (1999).
[0346] Alternatively the term "parent subtilase" may be termed
"wild type subtilase".
Modification(s) of a Subtilase Variant
[0347] The term "modification(s)" used herein is defined to include
chemical modification of a subtilase as well as genetic
manipulation of the DNA encoding a subtilase. The modification(s)
can be replacement(s) of the amino acid side chain(s),
substitution(s), deletion(s) and/or insertions in or at the amino
acid(s) of interest.
Subtilase Variant
[0348] In the context of this invention, the term subtilase variant
or mutated subtilase means a subtilase that has been produced by an
organism which is expressing a mutant gene derived from a parent
microorganism which possessed an original or parent gene and which
produced a corresponding parent enzyme, the parent gene having been
mutated in order to produce the mutant gene from which said mutated
subtilase protease is produced when expressed in a suitable
host.
[0349] Examples of relevant subtilisins comprise subtilisin BPN',
subtilisin amylosacchariticus, subtilisin 168, subtilisin
mesentericopeptidase, subtilisin Carlsberg, subtilisin DY,
subtilisin 309, subtilisin 147, PD498 (WO 93/24623), thermitase,
aqualysin, Bacillus PB92 protease, proteinase K, Protease TW7, and
Protease TW3.
[0350] Preferred commercially available protease enzymes include
Alcalase.TM., Savinase.TM. Primase.TM., Duralase.TM.,
Neutrase.RTM., Dyrazym.RTM., Esperase.TM., Pyrase.RTM., Pancreatic
Trypsin NOVO (PTN), Bio-Feed.TM. Pro, Clear-Lens Pro, and
Relase.RTM. (Novozymes A/S), Maxatase.TM. Maxacal.TM., Maxapem.TM.,
Properase.TM., Purafect.TM., Purafect OxP.TM. (Genencor
International Inc.).
[0351] It is to be understood that also protease variants are
contemplated as the parent protease. Examples of such protease
variants are disclosed in EP 130.756 (Genentech), EP 214.435
(Henkel), WO 87/04461 (Amgen), WO 87/05050 (Genex), EP 251.446
(Genencor), EP 260.105 (Genencor), Thomas et al., (1985), Nature.
318, p. 375-376, Thomas et al., (1987), J. Mol. Biol., 193, pp.
803-813, Russel et al., (1987), Nature, 328, p. 496-500, WO
88/08028 (Genex), WO 88/08033 (Amgen), WO 89/06279 (Novo Nordisk
A/S), WO 91/00345 (Novo Nordisk A/S), EP 525 610 (Solvay) and WO
94/02618 (Gist-Brocades N.V.).
[0352] The activity of proteases can be determined as described in
"Methods of Enzymatic Analysis", third edition, 1984, Verlag
Chemie, Weinheim, vol. 5.
Parent Lipolytic Enzymes
[0353] Lipolytic enzymes are classified in EC 3.1.1 Carboxylic
Ester Hydrolases according to Enzyme Nomenclature (available at
http://www.chem.qmw.ac.uk/iubmb/enzyme). The lipolytic enzyme may
have a substrate specificity with an activity such as EC 3.1.1.3
triacylglycerol lipase, EC 3.1.1.4 phospholipase A2, EC 3.1.1.5
lysophospholipase, EC 3.1.1.26 galactolipase, EC 3.1.1.32
phospholipase A1, EC 3.1.1.73 feruloyl esterase or EC 3.1.1.74
cutinase.
[0354] The parent lipolytic enzyme may be prokaryotic, particularly
a bacterial enzyme, e.g. from Pseudomonas. Examples are Pseudomonas
lipases, e.g. from P. cepacia (U.S. Pat. No. 5,290,694, pdb file
1OIL), P. glumae (N Frenken et al. (1992), Appl. Envir. Microbiol.
58 3787-3791, pdb files 1TAH and 1QGE), P. pseudoalcaligenes (EP
334 462) and Pseudomonas sp. strain SD 705 (FERM BP-4772) (WO
95/06720, EP 721 981, WO 96/27002, EP 812 910). The P. glumae
lipase sequence is identical to the amino acid sequence of
Chromobacterium viscosum (DE 3908131 A1). Other examples are
bacterial cutinases, e.g. from Pseudomonas such as P. mendocina
(U.S. Pat. No. 5,389,536) or P. putida (WO 88/09367).
[0355] Alternatively, the parent lipolytic enzyme may be
eukaryotic, e.g. a fungal lipolytic enzyme such as lipolytic
enzymes of the Humicola family and the Zygomycetes family and
fungal cutinases.
[0356] Examples of fungal cutinases are the cutinases of Fusarium
solani pisi (S. Longhi et al., Journal of Molecular Biology, 268
(4), 779-799 (1997)) and Humicola insolens (U.S. Pat. No.
5,827,719).
[0357] The parent lipolytic enzyme may be fungal and may have an
amino acid sequence that can be aligned with SEQ ID NO: 1 which is
the amino acid sequence shown in positions 1-269 of SEQ ID NO: 2 of
U.S. Pat. No. 5,869,438 for the lipase from Thermomyces lanuginosus
(synonym Humicola lanuginosa), described in EP 258 068 and EP 305
216 (trade name LIPOLASE). The parent lipolytic enzyme may
particularly have an amino acid sequence with at least 50% homology
with SEQ ID NO: 1. In addition to the lipase from T. lanuginosus,
other examples are a lipase from Penicillium camembertii (P25234),
a lipase from Fusasrium, lipase/phospholipase from Fusarium
oxysporum (EP 130064, WO 98/26057), lipase from F. heterosporum
(R87979), lysophospholipase from Aspergillus foetidus (W33009),
phospholipase A1 from A. oryzae (JP-A 10-155493), lipase from A.
oryzae (D85895), lipase/ferulic acid esterase from A. niger
(Y09330), lipase/ferulic acid esterase from A. tubingensis
(Y09331), lipase from A. tubingensis (WO 98/45453),
lysophospholipase from A. niger (WO 98/31790), lipase from F.
solanii having an isoelectric point of 6.9 and an apparent
molecular weight of 30 kDa (WO 96/18729).
[0358] Other examples are the Zygomycetes family of lipases
comprising lipases having at least 50% homology with the lipase of
Rhizomucor miehei (P19515. This family also includes the lipases
from Absidia reflexa, A. sporophora, A. corymbifera, A.
blakesleeana, A. griseola (all described in WO 96/13578 and WO
97/27276) and Rhizopus oryzae (P21811). Numbers in parentheses
indicate publication or accession to the EMBL, GenBank, GeneSeqp or
Swiss-Prot databases.
[0359] Examples of lipases include lipases derived from the
following microorganisms. The indicated patent publications are
incorporated herein by reference:
[0360] Humicola, e.g. H. brevispora, H. brevis var. thermoidea.
[0361] Pseudomonas, e.g. Ps. fragi, Ps. stutzeri, Ps. cepacia and
Ps. fluorescens (WO 89/04361), or Ps. plantarii or Ps. gladioli
(U.S. Pat. No. 4,950,417 (Solvay enzymes)) or Ps. alcaligenes and
Ps. pseudoalcaligenes (EP 218 272) or.
[0362] Candida, e.g. C. cylindracea (also called C. rugosa) or C.
antarctica (WO 88/02775) or C. antarctica lipase A or B (WO
94/01541 and WO 89/02916).
[0363] Geotricum, e.g. G. candidum (Schimada et al., (1989), J.
Biochem., 106, 383-388).
[0364] Rhizopus, e.g. R. delemar (Hass et al., (1991), Gene 109,
107-113) or R. niveus (Kugimiya et al., (1992) Biosci. Biotech.
Biochem 56, 716-719) or R. oryzae.
[0365] Bacillus, e.g. B. subtilis (Dartois et al., (1993)
Biochemica et Biophysica acta 1131, 253-260) or B.
stearothermophilus (JP 64/7744992) or B. pumilus (WO 91/16422).
[0366] Specific examples of readily available commercial lipases
include Lipolase.RTM. (WO 98/35026) Lipolase.TM. Ultra,
Lipozyme.RTM., Palatase.RTM., Novozym.RTM. 435, Lecitase.RTM. (all
available from Novozymes A/S).
[0367] Examples of other lipases are Lumafast.TM., Ps. mendocian
lipase from Genencor Int. Inc.; Lipomax.TM., Ps. pseudoalcaligenes
lipase from Gist Brocades/Genencor Int. Inc.; Fusarium solani
lipase (cutinase) from Unilever; Bacillus sp. lipase from Solvay
enzymes. Other lipases are available from other companies.
[0368] It is to be understood that also lipase variants are
contemplated as the parent enzyme. Examples of such are described
in e.g. WO 93/01285 and WO 95/22615.
[0369] The activity of the lipase can be determined as described in
"Methods of Enzymatic Analysis", Third Edition, 1984, Verlag
Chemie, Weinhein, vol. 4, or as described in AF 95/5 GB (available
on request from Novozymes A/S).
Parent Oxidoreductases
[0370] Parent oxidoreductases (i.e. enzymes classified under the
Enzyme Classification number E.C. 1 (Oxidoreductases) in accordance
with the Recommendations (1992) of the International Union of
Biochemistry and Molecular Biology (IUBMB)) include oxidoreductases
within this group.
[0371] Examples include oxidoreductases selected from those
classified under the Enzyme Classification (E.C.) numbers:
[0372] Glycerol-3-phosphate dehydrogenase (NAD) (1.1.1.8),
Glycerol-3-phosphate dehydrogenase [NAD(P)] (1.1.1.94),
Glycerol-3-phosphate 1-dehydrogenase [NADP] (1.1.1.94), Glucose
oxidase (1.1.3.4), Hexose oxidase (1.1.3.5), Catechol oxidase
(1.1.3.14), Bilirubin oxidase (1.3.3.5), Alanine dehydrogenase
(1.4.1.1), Glutamate dehydrogenase (1.4.1.2), Glutamate
dehydrogenase [NAD(P)] (1.4.1.3), Glutamate dehydrogenase (NADP)
(1.4.1.4), L-Amino acid dehydrogenase (1.4.1.5), Serine
dehydrogenase (1.4.1.7), Valine dehydrogenase (NADP) (1.4.1.8),
Leucine dehydrogenase (1.4.1.9), Glycine dehydrogenase (1.4.1.10),
L-Amino-acid oxidase (1.4.3.2.), D-Amino-acid oxidase(1.4.3.3),
L-Glutamate oxidase (1.4.3.11), Protein-lysine 6-oxidase
(1.4.3.13), L-lysine oxidase (1.4.3.14), L-Aspartate oxidase
(1.4.3.16), D-amino-acid dehydrogenase (1.4.99.1), Protein
disulfide reductase (1.6.4.4), Thioredoxin reductase (1.6.4.5),
Protein disulfide reductase (glutathione) (1.8.4.2), Laccase
(1.10.3.2), Catalase (1.11.1.6), Peroxidase (1.11.1.7),
Lipoxygenase (1.13.11.12), Superoxide dismutase (1.15.1.1).
[0373] Said glucose oxidases may be derived from Aspergillus
niger.
[0374] Said laccases may be derived from Polyporus pinsitus,
Myceliophthora thermophila, Coprinus cinereus, Rhizoctonia solani,
Rhizoctonia praticola, Scytalidium thermophilum and Rhus
vernicifera. Because of the homology found between the above
mentioned laccases (see WO 98/38287), they are considered to belong
to the same class of laccases, namely the class of "Coprinus-like
laccases". Accordingly, in the present context, the term
"Coprinus-like laccase" is intended to indicate a laccase which, on
the amino acid level, displays a homology of at least 50% and less
than 100% to the Coprinus cinereus laccase SEQ ID NO: 3, or at
least 55% and less than 100% to the Coprinus cinereus laccase SEQ
ID NO: 3, or at least 60% and less than 100% to the Coprinus
cinereus laccase SEQ ID NO: 3, or at least 65% and less than 100%
to the Coprinus cinereus laccase SEQ ID NO: 3, or at least 70% and
less than 100% to the Coprinus cinereus laccase SEQ ID NO: 3, or at
least 75% and less than 100% to the Coprinus cinereus laccase SEQ
ID NO: 3, or at least 80% and less than 100% to the Coprinus
cinereus laccase SEQ ID NO: 3, or at least 85% and less than 100%
to the Coprinus cinereus laccase SEQ ID NO: 3, or at least 90% and
less than 100% to the Coprinus cinereus laccase SEQ ID NO: 3, at
least 95% and less than 100% or at least 98% and less than 100% to
the Coprinus cinereus laccase SEQ ID NO: 3.
[0375] Bilirubin oxidases may be derived from Myrothechecium
verrucaria.
[0376] The peroxidase may be derived from e.g. Soy bean,
Horseradish or Coprinus cinereus.
[0377] The protein disulfide reductase may be any of the mentioned
in Danish application nos. 768/93, 265/94 and 264/94 (Novo Nordisk
A/S), which are hereby incorporated as references, including
Protein Disulfide reductases of bovine origin, Protein Disulfide
reductases derived from Aspergillus oryzae or Aspergillus niger,
and DsbA or DsbC derived from Escherichia coli.
[0378] Specific examples of readily available commercial
oxidoreductases include Gluzyme.TM. (enzyme available from
Novozymes A/S). However, other oxidoreductases are available from
others.
[0379] It is to be understood that also variants of oxidoreductases
are contemplated as the parent enzyme.
[0380] The activity of oxidoreductases can be determined as
described in "Methods of Enzymatic Analysis", third edition, 1984,
Verlag Chemie, Weinheim, vol. 3.
Parent Carbohydrases
[0381] Parent carbohydrases may be defined as all enzymes capable
of breaking down carbohydrate chains (e.g. starches) of especially
five and six member ring structures (i.e. enzymes classified under
the Enzyme Classification number E.C. 3.2 (glycosidases) in
accordance with the Recommendations (1992) of the International
Union of Biochemistry and Molecular Biology (IUBMB)). Also included
in the group of carbohydrases according to the invention are
enzymes capable of isomerizing carbohydrates e.g. six member ring
structures, such as D-glucose to e.g. five member ring structures
like D-fructose.
[0382] Examples include carbohydrases selected from those
classified under the Enzyme Classification (E.C.) numbers:
alpha-amylase (3.2.1.1), beta-amylase (3.2.1.2), glucan
1,4-alpha-glucosidase (3.2.1.3), cellulase (3.2.1.4),
endo-1,3(4)-beta-glucanase (3.2.1.6), endo-1,4-beta-xylanase
(3.2.1.8), dextranase (3.2.1.11), chitinase (3.2.1.14),
polygalacturonase (3.2.1.15), lysozyme (3.2.1.17), beta-glucosidase
(3.2.1.21), alpha-galactosidase (3.2.1.22), beta-galactosidase
(3.2.1.23), amylo-1,6-glucosidase (3.2.1.33), xylan
1,4-beta-xylosidase (3.2.1.37), glucan endo-1,3-beta-D-glucosidase
(3.2.1.39), alpha-dextrin endo-1,6-glucosidase (3.2.1.41), sucrose
alpha-glucosidase (3.2.1.48), glucan endo-1,3-alpha-glucosidase
(3.2.1.59), glucan 1,4-beta-glucosidase (3.2.1.74), glucan
endo-1,6-beta-glucosidase (3.2.1.75), arabinan
endo-1,5-alpha-arabinosidase (3.2.1.99), lactase (3.2.1.108),
chitonanase (3.2.1.132) and xylose isomerase (5.3.1.5).
[0383] Examples of relevant carbohydrases include
alpha-1,3-glucanases derived from Trichoderma harzianum;
alpha-1,6-glucanases derived from a strain of Paecilomyces;
beta-glucanases derived from Bacillus subtilis; beta-glucanases
derived from Humicola insolens; beta-glucanases derived from
Aspergillus niger, beta-glucanases derived from a strain of
Trichoderma; beta-glucanases derived from a strain of Oerskovia
xanthineolytica; exo-1,4-alpha-D-glucosidases (glucoamylases)
derived from Aspergillus niger; alpha-amylases derived from
Bacillus subtilis; alpha-amylases derived from Bacillus
amyloliquefaciens; alpha-amylases derived from Bacillus
stearothermophilus; alpha-amylases derived from Aspergillus oryzae;
alpha-amylases derived from non-pathogenic microorganisms;
alpha-galactosidases derived from Aspergillus niger, Pentosanases,
xylanases, cellobiases, cellulases, hemi-cellulases derived from
Humicola insolens; cellulases derived from Trichoderma reesei;
cellulases derived from non-pathogenic mold; pectinases,
cellulases, arabinases, hemi-celluloses derived from Aspergillus
niger, dextranases derived from Penicillium lilacinum;
endo-glucanase derived from non-pathogenic mold; pullulanases
derived from Bacillus acidopullyticus; beta-galactosidases derived
from Kluyveromyces fragilis; xylanases derived from Trichoderma
reesei.
[0384] Specific examples of readily available commercial
carbohydrases include Alpha-Gal.TM. Bio-Feed.TM. Alpha,
Bio-Feed.TM. Beta, Bio-Feed.TM. Plus, Bio-Feed.TM. Plus,
Novozyme.RTM. 188, Carezyme.RTM. (SEQ ID NO: 5), Celluclast.RTM.,
Cellusoft.RTM., Ceremyl.RTM., Citrozym.TM., Denimax.TM. Dezyme.TM.,
Dextrozyme.TM., Finizym.RTM., Fungamyl.TM., Gamanase.TM.,
Glucanex.RTM., Lactozym.RTM., Maltogenase.TM., Pentopan.TM.,
Pectinex.TM., Promozyme.RTM., Pulpzyme.TM., Novamyl.TM.,
Termamyl.RTM., AMG (Amyloglucosidase Novo), Maltogenase.RTM.,
Sweetzyme.RTM., Aquazym.RTM., Natalase.RTM. (SEQ ID NO: 4), SP722,
AA560 (all enzymes available from Novozymes A/S). Other
carbohydrases are available from other companies.
[0385] The parent cellulase is preferably a microbial cellulase. As
such, the cellulase may be selected from bacterial cellulases, e.g.
Pseudomonas cellulases or Bacillus, such as the Bacillus strains
described in U.S. Pat. No. 4,822,516, U.S. Pat. No. 5,045,464 or EP
468 464, or B. lautus (cf. WO 91/10732), cellulases. More
preferably, the parent cellulases may be a fungal cellulase, in
particular Humicola, Trichoderma, Irpex, Aspergillus, Penicillium,
Myceliophthora or Fusarium cellulases. Examples of suitable parent
cellulases are described in, e.g. WO 91/17244. Examples of suitable
Trichoderma cellulases are those described in T. T. Teeri, Gene 51,
1987, pp. 43-52. Preferably, the parent cellulase is selected from
the cellulases classified in family 45, e.g. the enzymes EG B
(Pseudomonas fluorescens) and EG V (Humicola insolens), as
described in Henrissat, B. et al.: Biochem. J. (1993), 293, p.
781-788.
The Termamyl-Like Alpha-Amylase
[0386] It is well known that a number of alpha-amylases produced by
Bacillus spp. are highly homologous on the amino acid level. For
instance, the B. licheniformis alpha-amylase comprising the amino
acid sequence shown in SEQ ID NO: 4 of WO 00/29560 (commercially
available as Termamyl.RTM.) has been found to be about 89%
homologous with the B. amyloliquefaciens alpha-amylase comprising
the amino acid sequence shown in SEQ ID NO: 5 of WO 00/29560 and
about 79% homologous with the B. stearothermophilus alpha-amylase
comprising the amino acid sequence shown in SEQ ID NO: 3 of WO
00/29560. Further homologous alpha-amylases include an
alpha-amylase derived from a strain of the Bacillus sp. NCIB 12289,
NCIB 12512, NCIB 12513 or DSM 9375, all of which are described in
detail in WO 95/26397, and the alpha-amylase described by Tsukamoto
et al., Biochemical and Biophysical Research Communications, 151
(1988), pp. 25-31.
[0387] Still further homologous alpha-amylases include the
alpha-amylase produced by the B. licheniformis strain described in
EP 0252666 (ATCC 27811), and the alpha-amylases identified in WO
91/00353 and WO 94/18314. Other commercial Termamyl-like B.
licheniformis alpha-amylases are Optitherm.RTM. and Takatherm.RTM.
(available from Solvay), Maxamyl.RTM. (available from
Gist-brocades/Genencor), Spezym AA.RTM. and Spezyme Delta AA.TM.
(available from Genencor), and Keistase.RTM. (available from
Daiwa).
[0388] Because of the substantial homology found between these
alpha-amylases, they are considered to belong to the same class of
alpha-amylases, namely the class of "Termamyl-like
alpha-amylases".
[0389] Accordingly, in the present context, the term "Termamyl-like
alpha-amylase" is intended to indicate an alpha-amylase which, at
the amino acid level, exhibits a substantial homology to
Termamyl.RTM., i.e., the B. licheniformis alpha-amylase having the
amino acid sequence shown in SEQ ID NO: 4 (WO 00/29560). In other
words, a Termamyl-like alpha-amylase is an alpha-amylase which has
the amino acid sequence shown in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7 or
8 of WO 00/29560, and the amino acid sequence shown in SEQ ID NO: 1
of WO 95/26397 (the same as the amino acid sequence shown as SEQ ID
NO: 7 of WO 00/29560) or in SEQ ID NO: 2 of WO 95/26397 (the same
as the amino acid sequence shown as SEQ ID NO: 8 of WO 00/29560) or
in Tsukamoto et al., 1988, (which amino acid sequence is shown in
SEQ ID NO: 6 of WO 00/29560) or i) which displays at least 60%
homology (identity), preferred at least 70%, more preferred at
least 75%, even more preferred at least 80%, especially at least
85%, especially preferred at least 90%, especially at least 95%,
even especially more preferred at least 97%, especially at least
99% homology with at least one of said amino acid sequences shown
in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7 or 8 of WO 00/29560 and/or ii)
displays immunological cross-reactivity with an antibody raised
against one or more of said alpha-amylases, and/or iii) is encoded
by a DNA sequence which hybridizes, under the low to very high
stringency conditions (said conditions described below) to the DNA
sequences encoding the above-specified alpha-amylases which are
apparent from SEQ ID NOS: 9, 10, 11, 12, and 32, respectively, of
the present application (which encodes the amino acid sequences
shown in SEQ ID NOS: 1, 2, 3, 4, and 5 herein, respectively), from
SEQ ID NO: 4 of WO 95/26397 (which DNA sequence, together with the
stop codon TAA, is shown in SEQ ID NO: 13 herein and encodes the
amino acid sequence shown in SEQ ID NO: 8 herein) and from SEQ ID
NO: 5 of WO 95/26397 (shown in SEQ ID NO: 14 herein),
respectively.
[0390] In connection with property i), the "homology" (identity)
may be determined by use of any conventional algorithm, preferably
by use of the gap progamme from the GCG package version 8 (August
1994) using default values for gap penalties, i.e., a gap creation
penalty of 3.0 and gap extension penalty of 0.1 (Genetic Computer
Group (1991) Programme Manual for the GCG Package, version 8, 575
Science Drive, Madison, Wis., USA 53711).
[0391] The parent Termamyl-like alpha-amylase backbone may in an
embodiment have an amino acid sequence which has a degree of
identity to SEQ ID NO: 4 (WO 00/29560) of at least 65%, preferably
at least 70%, preferably at least 75%, more preferably at least
80%, more preferably at least 85%, even more preferably at least
about 90%, even more preferably at least 95%, even more preferably
at least 97%, and even more preferably at least 99% identity
determined as described above.
[0392] A structural alignment between Termamyl.RTM. (SEQ ID NO: 4)
and a Termamyl-like alpha-amylase may be used to identify
equivalent/corresponding positions in other Termamyl-like
alpha-amylases. One method of obtaining said structural alignment
is to use the Pile Up programme from the GCG package using default
values of gap penelties, i.e., a gap creation penalty of 3.0 and
gap extension penalty of 0.1. Other structural alignment methods
include the hydrophobic cluster analysis (Gaboriaud et al., (1987),
FEBS LETTERS 224, pp. 149-155) and reverse threading (Huber, T;
Torda, A E, PROTEIN SCIENCE Vol. 7, No. 1 pp. 142-149 (1998).
Parent Glucoamylases
[0393] Parent glucoamylase contemplated according to the present
invention include fungal glucoamylases, in particular fungal
glucoamylases obtainable from an Aspergillus strain, such as an
Aspergillus niger or Aspergillus awamori glucoamylases and variants
or mutants thereof, homologous glucoamylases, and further
glucoamylases being structurally and/or functionally similar to SEQ
ID NO: 2 (WO 00/04136). Specifically contemplated are the
Aspergillus niger glucoamylases G1 and G2 disclosed in Boel et al.
(1984), "Glucoamylases G1 and G2 from Aspergillus niger are
synthesized from two different but closely related mRNAs", EMBO J.
3 (5), p. 1097-1102. The G2 glucoamylase is disclosed in SEQ ID NO:
2 (WO 00/04136). The G1 glucoamylase is disclosed in SEQ ID NO: 13
(WO 00/04136). Another AMG backbone contemplated is Talaromyces
emersonii, especially Talaromyces emersonii DSM disclosed in WO
99/28448 (Novo Nordisk).
[0394] The homology referred to above of the parent glucoamylase is
determined as the degree of identity between two protein sequences
indicating a derivation of the first sequence from the second. The
homology may suitably be determined by means of computer programs
known in the art such as GAP provided in the GCG program package
(Program Manual for the Wisconsin Package, Version 8, August 1994,
Genetics Computer Group, 575 Science Drive, Madison, Wis., USA
53711) (Needleman, S. B. and Wunsch, C. D., (1970), Journal of
Molecular Biology, 48, p. 443-453). Using Gap with the following
settings for polypeptide sequence comparison: Gap creation penalty
of 3.0 and Gap extension penalty of 0.1, the mature part of a
polypeptide encoded by an analogous DNA sequence of the invention
exhibits a degree of identity preferably of at least 60%, such as
70%, at least 80%, at least 90%, more preferably at least 95%, more
preferably at least 97%, and most preferably at least 99% with the
mature part of the amino acid sequence shown in SEQ ID NO: 2 (WO
00/04136).
[0395] Preferably, the parent glucoamylase comprise the amino acid
sequences of SEQ ID NO: 2 (WO 00/04136); or allelic variants
thereof; or fragments thereof that has glucoamylase activity.
[0396] A fragment of SEQ ID NO: 2 is a polypeptide which have one
or more amino acids deleted from the amino and/or carboxyl terminus
of this amino acid sequence. For instance, the AMG G2 (SEQ ID NO:
2) is a fragment of the Aspergillus niger G1 glucoamylase (Boel et
al. (1984), EMBO J. 3 (5), p. 1097-1102) having glucoamylase
activity. An allelic variant denotes any of two or more alternative
forms of a gene occupying the same chromosomal locus. Allelic
variation arises naturally through mutation, and may result in
polymorphism within populations. Gene mutations can be silent (no
change in the encoded polypeptide) or may encode polypeptides
having altered amino acid sequences. An allelic variant of a
polypeptide is a polypeptide encoded by an allelic variant of a
gene.
[0397] It is to be understood that also carbohydrase variants are
contemplated as the parent enzyme.
[0398] The activity of carbohydrases can be determined as described
in "Methods of Enzymatic Analysis", third edition, 1984, Verlag
Chemie, Weinheim, vol. 4.
Parent Transferases
[0399] Parent transferases (i.e. enzymes classified under the
Enzyme Classification number E.C. 2 in accordance with the
Recommendations (1992) of the International Union of Biochemistry
and Molecular Biology (IUBMB)) include transferases within this
group.
[0400] The parent transferases may be any transferase in the
subgroups of transferases: transferases transferring one-carbon
groups (E.C. 2.1); transferases transferring aldehyde or residues
(E.0 2.2); acyltransferases (E.C. 2.3); glucosyltransferases (E.C.
2.4); transferases transferring alkyl or aryl groups, other that
methyl groups (E.C. 2.5); transferases transferring nitrogeneous
groups (2.6).
[0401] In a preferred embodiment the parent transferase is a
transglutaminase E.C 2.3.2.13 (Protein-glutamine
.mu.-glutamyltransferase).
[0402] Transglutaminases are enzymes capable of catalyzing an acyl
transfer reaction in which a gamma-carboxyamide group of a
peptide-bound glutamine residue is the acyl donor. Primary amino
groups in a variety of compounds may function as acyl acceptors
with the subsequent formation of monosubstituted gamma-amides of
peptide-bound glutamic acid. When the epsilon-amino group of a
lysine residue in a peptide-chain serves as the acyl acceptor, the
transferases form intramolecular or intermolecular
gamma-glutamyl-epsilon-lysyl crosslinks.
[0403] Examples of transglutaminases are described in the pending
DK patent application no. 990/94 (Novo Nordisk A/S).
[0404] The parent transglutaminase may be of human, animal (e.g.
bovine) or microbial origin.
[0405] Examples of such parent transglutaminases are animal derived
Transglutaminase, FXIIIa; microbial transglutaminases derived from
Physarum polycephalum (Klein et al., Journal of Bacteriology, Vol.
174, p. 2599-2605); transglutaminases derived from Streptomyces
sp., including Streptomyces lavendulae, Streptomyces lydicus
(former Streptomyces libani) and Streptoverticillium sp., including
Streptoverticillium mobaraense, Streptoverticillium cinnamoneum,
and Streptoverticillium griseocarneum (Motoki et al., U.S. Pat. No.
5,156,956; Andou et al., U.S. Pat. No. 5,252,469; Kaempfer et al.,
Journal of General Microbiology, Vol. 137, p. 1831-1892; Ochi et
al., International Journal of Sytematic Bacteriology, Vol. 44, p.
285-292; Andou et al., U.S. Pat. No. 5,252,469; Williams et al.,
Journal of General Microbiology, Vol. 129, p. 1743-1813).
[0406] It is to be understood that also transferase variants are
contemplated as the parent enzyme.
[0407] The activity of transglutaminases can be determined as
described in "Methods of Enzymatic Analysis", third edition, 1984,
Verlag Chemie, Weinheim, vol. 1-10.
Parent Phytases
[0408] Parent phytases are included in the group of enzymes
classified under the Enzyme Classification number E.C. 3.1.3
(Phosphoric Monoester Hydrolases) in accordance with the
Recommendations (1992) of the International Union of Biochemistry
and Molecular Biology (IUBMB)).
[0409] Phytases are enzymes produced by microorganisms which
catalyse the conversion of phytate to inositol and inorganic
phosphorus
[0410] Phytase producing microorganisms comprise bacteria such as
Bacillus subtilis, Bacillus natto and Pseudomonas; yeasts such as
Saccharomyces cerevisiae; and fungi such as Aspergillus niger,
Aspergillus ficuum, Aspergillus awamori, Aspergillus oryzae,
Aspergillus terreus or Aspergillus nidulans, and various other
Aspergillus species).
[0411] Examples of parent phytases include phytases selected from
those classified under the Enzyme Classification (E.C.) numbers:
3-phytase (3.1.3.8) and 6-phytase (3.1.3.26).
[0412] The activity of phytases can be determined as described in
"Methods of Enzymatic Analysis", third edition, 1984, Verlag
Chemie, Weinheim, vol. 1-10, or may be measured according to the
method described in EP-A1-0 420 358, Example 2A.
Lyases
[0413] Suitable lyases include Polysaccharide lyases: Pectate
lyases (4.2.2.2) and pectin lyases (4.2.2.10), such as those from
Bacillus licheniformis disclosed in WO 99/27083.
Isomerases
Protein Disulfide Isomerase
[0414] Without being limited thereto suitable protein disulfide
isomerases include PDIs described in WO 95/01425 (Novo Nordisk A/S)
and suitable glucose isomerases include those described in
Biotechnology Letter, Vol. 20, No 6, June 1998, pp. 553-56.
[0415] Contemplated isomerases include xylose/glucose Isomerase
(5.3.1.5) including Sweetzyme.RTM..
Environmental Allergens
[0416] The environmental allergens that are of interest for epitope
mapping include allergens from pollen, dust mites, mammals, venoms,
fungi, food items, and other plants.
[0417] Pollen, allergens include but are not limited to those of
the order Fagales, Oleales, Pinales, Poales, Asterales, and
Urticales; including those from Betula, Alnus, Corylus, Carpinus,
Olea, Phleum pratense and Artemisia vulgaris, such as Aln g1, Cor
a1, Car b1, Cry j1, Amb a1 and a2, Art v1, Par j1, Ole e1, Ave v1,
and Bet v1 (WO 99/47680).
[0418] Mite allergens include but are not limited to those from
Derm. farinae and Derm. pteronys., such as Der f1 and f2, and Der
p1 and p2.
[0419] From mammals, relevant environmental allergens include but
are not limited to those from cat, dog, and horse as well as from
dandruff from the hair of those animals, such as Fel d1; Can f1;
Equ c1; Equ c2; Equ c3.
[0420] Venum allergens include but are not limited to PLA2 from bee
venom as well as Apis m1 and m2, Ves g1, g2 and g5, Ves v5 and to
Pol and Sol allergens.
[0421] Fungal allergens include those from Alternaria alt. and
Cladospo. herb. such as Alt a1 and Cla h1.
[0422] Food allergens include but are not limited to those from
milk (lactoglobulin), egg (ovalbumin), peanuts, hazelnuts, wheat
(alpha-amylase inhibitor),
[0423] Other plant allergens include latex (hevea
brasiliensis).
[0424] In addition, a number of proteins of interest for expression
in transgenic plants could be useful objects for epitope
engineering. If for instance a heterologous enzyme is introduced
into a transgenic plant e.g. to increase the nutritional value of
food or feed derived from that plant, that enzyme may lead to
allergenicity problems in humans or animals ingesting the
plant-derived material. Epitope mapping and engineering of such
heterologous enzymes or other proteins of transgenic plants may
lead to reduction or elimination of this problem. Hence, the
methods of this patent are also useful for potentially modifying
proteins for heterologous expression in plants and plant cells.
Materials and Methods
Materials
ELISA Reagents:
[0425] Horse Radish Peroxidase labelled pig anti-rabbit-Ig (Dako,
DK, P217, dilution 1:1000) Rat anti-mouse IgE (Serotec MCA419;
dilution 1:100) Mouse anti-rat IgE (Serotec MCA193; dilution 1:200)
Biotin-labelled mouse anti-rat IgG1 monoclonal antibody (Zymed
03-9140; dilution 1:1000) Biotin-labelled rat anti-mouse IgG1
monoclonal antibody (Serotec MCA336B; dilution 1:2000)
Streptavidin-horse radish peroxidase (Kirkegard & Perry
14-30-00; dilution 1:1000).
Buffers and Solutions:
[0426] PBS (pH 7.2 (1 liter))
TABLE-US-00001 NaCl 8.00 g KCl 0.20 g K.sub.2HPO.sub.4 1.04 g
KH.sub.2PO.sub.4 0.32 g
[0427] Washing buffer PBS, 0.05% (v/v) Tween 20
[0428] Blocking buffer PBS, 2% (wt/v) Skim Milk powder
[0429] Dilution buffer PBS, 0.05% (v/v) Tween 20, 0.5% (wt/v) Skim
Milk powder
[0430] Citrate buffer 0.1M, pH 5.0-5.2
[0431] Stop-solution (DMG-buffer)
[0432] Sodium Borate, borax (Sigma)
[0433] 3,3-Dimethyl glutaric acid (Sigma)
[0434] Tween 20: Poly oxyethylene sorbitan mono laurate (Merck cat
no. 822184)
[0435] PMSF (phenyl methyl sulfonyl flouride) from Sigma
[0436]
Succinyl-Alanine-Alanine-Proline-Phenylalanine-paranitro-anilide
(Suc-AAPF-pNP) Sigma no. S-7388, Mw 624.6 g/mol.
[0437] mPEG (Fluka)
Coloring Substrate:
[0438] OPD: o-phenylene-diamine, (Kementec cat no. 4260)
Methods
Automatic Epitope Mapping
Implementation
[0439] The implementation consists of 3 pieces of code:
1. The core program (see above), written in C (see Appendix A). 2.
A "wrapping" cgi-script run by the web server, written in Python
(see Appendix B). 3. A HTML page defining the input/submission form
(see Appendix C).
[0440] The wrapper receives the input and calls the core program
and several other utilities.
Apart from the standard Unix utility programs (my, rm, awk, etc.)
the following must be installed:
[0441] A web server capable of running cgi-scripts, e.g. Apache
[0442] Python 1.5 or later
[0443] Gnuplot 3.7 or later
[0444] DSSP, version July 1995
The Core Program
Inputs
[0445] 1. A Brookhaven PDB file with the structure of the protein
2. The output of DSSP called with the above PDB file. 3. Maximum
distance between adjacent residues 4. Minimum solvent accessible
surface area for each residue 5. Maximum epitope size (max distance
between any two residues in epitope) 6. Maximum number of
non-redundant epitopes to include (0=all) 7. The shortest
acceptable epitope (as a fraction of the length of the epitope
consensus sequence). 8. Epitope consensus sequence describing which
residues are possible at the different positions. An example is
shown below: KR (Lys or Arg allowed) AILV- (Ala, Ile, Leu, Val or
missing residue allowed) * (All Residues Allowed, but there Must be
a Residue)
? (All or Missing Residue Allowed)
[0446] DE (Asp or Glu allowed) (*, ? or - in first or last position
is allowed but obsolete. (- in first position is ignored.))
[0447] Examples of matching epitopes:
KAAKD (SEQ ID NO: 41), KLASD (SEQ ID NO: 42), KLYSD (SEQ ID NO:
43), KLY-D (SEQ ID NO: 44), R-M-D.
The Epitope Searching Algorithm
[0448] The "core" of the program is the algorithm that scans the
protein surface for the epitope patterns. The principle is that
several "trees" are built, where each of their branches describes
one epitope:
1. All residues in the protein are checked according to: a) Does
the residue type match the first residue of the epitope consensus
sequence. b) Is the surface accessibility greater than or equal to
the given threshold. If both requirements are fulfilled, the
protein residue is considered as one root in the epitope tree.
Remark that there are usually many roots. 2. For each of the
residues defined as roots, all residues within the given threshold
distance between adjacent residues (e.g. 7 Angstroms) are checked
for the same as above: a) Does the residue type match the second
residue of the epitope consensus sequence. b) Is the surface
accessibility greater than or equal to the given threshold. If yes,
the protein residue is considered as a "child" of the root. The
spatial position of a residue is defined as the coordinates of its
C-alpha atom. 3. The procedure from step 2 is repeated for the next
residue in the epitope consensus sequence, where each of the
"childs" found in step 2 are now "roots" of new childs. If a gap is
defined in the epitope consensus sequence, a "missing" residue is
allowed, and the coordinates of the root (also called "parent") is
used. 4. This procedure is repeated for all residues in the epitope
consensus sequence. 5. In this way a number of trees (corresponding
to the number of roots found in step 1) are found. Notice that the
same protein residue can be present many places in the trees. 6. If
no epitopes that matches the length of the epitope consensus
sequence are found, the longest shorter epitopes that matches the
first n residues of the epitope consensus sequence are used, where
n is an integer smaller than the length of the epitope consensus
sequence. If n is smaller than the length of the epitope consensus
sequence multiplied by the fraction value defining the shortest
acceptable epitope length, no epitopes are written to the output,
and steps 7, 8 and 9 are skipped. 7. The epitopes are extracted
from the trees by traversing down from each of the "childs" in the
last level. The algorithm also finds epitopes which have the same
protein residue present more than once. This is, of course, an
artifact and such epitopes are discarded. Every epitope is then
checked for its size, that is, the maximum distance between any two
residues which are members of the epitope. If this exceeds the
threshold, the epitope is discarded. 8. Redundant epitopes are
removed. Epitopes containing one or more gaps are redundant if they
are subsets of other epitopes without or with fewer gaps. For
example: A82-gap-F45-G44-K43 is a subset of A82-L46-F45-G44-K43,
and is therefore discarded. 9. For every epitope, the total solvent
accessible surface area is calculated (by adding the contributions
from each residue as found by the DSSP program). The epitopes are
sorted according to this area in descending order. If a maximum
number of n non-redundant epitopes has been specified, the n
epitopes with largest solvent accessible surface area are selected.
10. The output consists of a list of the found epitopes, along with
information of the epitope consensus sequence used and other
internal parameters. A separate file containing the number of
epitopes that each of the protein residues is a member of is also
written. The wrapper
Inputs
[0449] 1. One PDB file, describing one structure, or one ZIP file,
containing a number of PDB files, each describing one structure.
The ZIP file must not contain subfolders. 2. An epitope consensus
sequence or which part of the current epitope library to use (full
library or IgE part or IgG part). 3. Maximum distance between
adjacent residues 4. Minimum solvent accessible surface area for
each residue 5. Maximum epitope size (max distance between any two
residues in epitope) 6. Maximum number of non-redundant epitopes to
include (0=all) 7. Whether to use sequential numbering (1,2,3,4,
etc) or PDB-file numbering.
DESCRIPTION
[0450] The core program accepts only one structure and one epitope
consensus sequence. It is usually desirable to use a library of
epitope consensus sequences and sometimes several protein
structures. The wrapper reads the user input and calls the utility
programs and the core program the necessary number of times. The
output is collected and presented on the web page returned to the
user.
[0451] Depending on the type of input, the wrapper works in
different modes:
[0452] Epitope consensus can be given directly or taken from a
library
[0453] Input type can be a single PDB file or a collection of PDB
file given as a ZIP-file.
[0454] Any of the four possible combinations are allowed.
[0455] The epitope library consists of a number of text files, each
containing one epitope consensus sequence as specified above.
[0456] The layout of the wrapper is like this:
1. Check if the program is already in use from somewhere else (this
is done by checking for a lock file when the wrapper starts. If it
does not exist, it is created and removed again when the program is
finished). 2. If the epitope consensus sequences are to be read
from the library, make an internal list of the desired library
entries. 3. If the input type is a ZIP file, unzip the file and
create one new directory for each of the contained PDB files. Move
each PDB file to its corresponding directory. 4. Do a loop over the
structures and/or epitope consensus sequences. For each
structure/epitope consensus sequence pair, DSSP and the core
program is called with the required parameters. If the input type
is a ZIP file, the outputs are put in the appropriate directories.
5. If the epitope library is used, a sum file containing the total
number of epitopes each residue is a member of. (Such a file is
generated by the core program for each epitope consensus
sequence--here a sum of these files is calculated). If input type
is a ZIP file, a sum file is generated for each structure and put
in the appropriate directory. 6. If the epitope library is used, a
file containing the total number of epitopes found from each entry
in the epitope library. If the input type is a PDB file, the file
contains only one line (with a number of data corresponding to the
library size). If the input type is a ZIP file, there is one line
for each structure. 7. Depending on the combination of input type
(ZIP or single PDB) and epitope consensus sequence source (typed-in
or epitope library), different information is returned to the user:
Single PDB+typed in epitope: Graph of numbers of epitopes that each
residue is a member of. List of found epitopes. ZIP file+typed in
epitope: Graphs (one for each structure) of numbers of epitopes
that each residue is a member of. Lists (one for each structure) of
found epitopes. Single PDB+epitope library: Graph of numbers of
epitopes that each residue is a member of (total for the complete
library). ZIP file+epitope library: Graphs (one for each structure)
of numbers of epitopes that each residue is a member of (total for
the complete library). Data flow sheets for the four different are
shown in the FIG. 8. For all modes except Single PDB+typed in
epitope, a ZIP file containing all output files is created and
returned to the user.
Immunisation of Brown Norway Rats:
[0457] Twenty intratracheal (IT) immunisations were performed
weekly with 0,100 ml 0.9% (wt/vol) NaCl (control group), or 0,100
ml of a protein dilution (.about.0, 1-1 mg/ml). Each group
contained 10 rats. Blood samples (2 ml) were collected from the eye
one week after every second immunisation. Serum was obtained by
blood clothing and centrifugation and analysed as indicated
below.
Immunisation of Balb/C Mice:
[0458] Twenty subcutaneous (SC) immunisations were performed weekly
with 0.05 ml 0.9% (wt/vol) NaCl (control group), or 0.050 ml of a
protein dilution (.about.0.01-0.1 mg/ml). Each group contained 10
female Balb/C mice (about 20 grams) purchased from Bomholdtgaard,
Ry, Denmark. Blood samples (0.100 ml) were collected from the eye
one week after every second immunisation. Serum was obtained by
blood clothing and centrifugation and analysed as indicated
below.
ELISA Procedure for Detecting Serum Levels of IgE and IgG:
[0459] Specific IgG1 and IgE levels were determined using the ELISA
specific for mouse or rat IgG1 or IgE. Differences between data
sets were analysed by using appropriate statistical methods.
Activation of CovaLink Plates:
[0460] A fresh stock solution of cyanuric chloride in acetone (10
mg/ml) is diluted into PBS, while stirring, to a final
concentration of 1 mg/ml and immediately aliquoted into CovaLink
NH2 plates (100 microliter per well) and incubated for 5 minutes at
room temperature. After three washes with PBS, the plates are dryed
at 50.degree. C. for 30 minutes, sealed with sealing tape, and
stored in plastic bags at room temperature for up to 3 weeks.
[0461] Mouse anti-Rat IgE was diluted 200.times. in PBS (5
microgram/ml). 100 microliter was added to each well. The plates
were coated overnight at 4.degree. C.
[0462] Unspecific adsorption was blocked by incubating each well
for 1 hour at room temperature with 200 microliter blocking buffer.
The plates were washed 3.times. with 300 microliter washing
buffer.
[0463] Unknown rat sera and a known rat IgE solution were diluted
in dilution buffer: Typically 10.times., 20.times. and 40.times.
for the unknown sera, and 1/2 dilutions for the standard IgE
starting from 1 .mu.g/ml. 100 microliter was added to each well.
Incubation was for 1 hour at room temperature.
[0464] Unbound material was removed by washing 3.times. with
washing buffer. The anti-rat IgE (biotin) was diluted 2000.times.
in dilution buffer. 100 microliter was added to each well.
Incubation was for 1 hour at room temperature. Unbound material was
removed by washing 3.times. with washing buffer.
[0465] Streptavidin was diluted 1000.times. in dilution buffer. 100
microliter was added to each well. Incubation was for 1 hour at
room temperature. Unbound material was removed by washing 3.times.
with 300 microliter washing buffer. OPD (0.6 mg/ml) and
H.sub.2O.sub.2 (0.4 microliter/ml) were dissolved in citrate
buffer. 100 microliter was added to each well. Incubation was for
30 minutes at room temperature. The reaction was stopped by
addition of 100 microliter H.sub.2SO.sub.4. The plates were read at
492 nm with 620 nm as reference.
[0466] Similar determination of IgG can be performed using anti
Rat-IgG and standard rat IgG reagents.
[0467] Similar determinations of IgG and IgE in mouse serum can be
performed using the corresponding species-specific reagents.
Direct IgE Assay:
[0468] To determine the IgE binding capacity of protein variants
one can use an assay, essentially as described above, but using
sequential addition of the following reagents:
1) Mouse anti-rat IgE antibodies coated in wells; 2) Known amounts
of rat antiserum containing IgE against the parent protein; 3)
Dilution series of the protein variant in question (or parent
protein as positive control); 4) Rabbit anti-parent antibodies 5)
HRPO-labelled anti-rabbit Ig antibodies for detection using OPD as
described.
[0469] The relative IgE binding capacity (end-point and/or
affinity) of the protein variants relative to that of the parent
protein are determined from the dilution-response curves. The
IgE-positive serum can be of other animals (including humans that
inadvertently have been sensitized to the parent protein) provided
that the species-specific anti-IgE capture antibodies are changed
accordingly.
Competitive ELISA (C-ELISA):
[0470] C-ELISA was performed according to established procedures.
In short, a 96 well ELISA plate was coated with the parent protein.
After proper blocking and washing, the coated antigen was incubated
with rabbit anti-enzyme polyclonal antiserum in the presence of
various amounts of modified protein (the competitior). The residual
amount of rabbit antiserum was detected by horseraddish
peroxidase-labelled pig anti-rabbit immunoglobulin.
Protein Sequences and Alignments:
[0471] For purposes of the present invention, the degree of
homology may be suitably determined by means of computer programs
known in the art, such as GAP provided in the GCG program package
(Program Manual for the Wisconsin Package, Version 8, August 1994,
Genetics Computer Group, 575 Science Drive, Madison, Wis., USA
53711) (Needleman, S. B. and Wunsch, C. D., (1970), Journal of
Molecular Biology, 48, 443-45).
Subtilisin Proteases:
[0472] In the present invention, corresponding (or homologous)
positions in subtilisin protease sequences are defined by alignment
with Subtilisin Novo (BPN') from B. amyloliquefaciens, as shown in
Table 1A for Alcalase, Protease B, Esperase, Protease C, Protease
D, Protease E, Protease A, PD498, Properase, Relase, Savinase.
TABLE-US-00002 TABLE 1A Alignment of different proteases to the
sequence of BPN' Alcalase: 69.5% identity in 275 residues overlap;
Score: 953.0; Gap frequency: 0.4% Alcalase, 1
AQTVPYGIPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYN-TD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD **
**** *** ** * ****** * ** ***** * **** * * * * Alcalase, 60
GNGHGTHVAGTVAALDNTTGVLGVAPSVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMD BPN',
61 DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
************ * ******** ******* *** ** * ***** * ** Alcalase, 120
VINMSLGGASGSTAMKQAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAV BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV
******** *** * * *** * * ********** ** * * *** ** ********
Alcalase, 180
DSNSNRASFSSVGAELEVMAPGAGVYSTYPTNTYATLNGTSMASPHVAGAAALILSKHPN BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN **
******** ** ***** ** * * * *********************** Alcalase, 240
LSASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ (SEQ ID NO: 45) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) *** * * * **
*********** **** Protease B: 59.6% identity in 275 residues
overlap; Score: 820.0; Gap frequency: 2.2% PROTEASE B, 1
AQTIPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD ** *
* * *** * * *** ***** * ** * **** **** ** * * ** PROTEASE B, 59
GNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMH BPN',
61 DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *************** ******** *** * * * *** * * PROTEASE B, 119
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** PROTEASE B, 175
DQNNNRASFSQYGAGLDIMAPGVNIQSTYPGSTYASDNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** ***** **** ** * ****** ********* * * PROTEASE B, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 47) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * ** Esperase: 54.7% identity in 274 residues overlap;
Score: 745.0; Gap frequency: 2.2% Esperase, 1
QTVPWGISFINTQQAHNRGIFGNGARVAVLDTGI-ASHPDLRIAGGASFISSE-PSYHDN BPN',
2 QSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQDD * **
* * * * * * *** * ** ***** ***** ** * * Esperase, 59
NGHGTHVAGTIAALNNSIGVLGVAPSADLYAVKVLDRNGSGSLASVAQGIEWAINNNMHI BPN',
62 NSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMDV *
******** *************** ******* *** ****** *** Esperase, 119
INMSLGSTSGSSTLELAVNRANNAGILLVGAAGNTGRQG----VNYPARYSGVMAVAAVD BPN',
122 INMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAVD
****** *** * ** * * * **** * * * ** * * ** *** Esperase, 175
QNGQRASFSTYGPEIEISAPGVNVNSTYTGNRYVSLSGTSMATPHVAGVAALVKSRYPSY BPN',
182 SSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNW
****** *** **** ** ** * ***** ***** *** * * Esperase, 235
TNNQIRQRINQTATYLGSPSLYGNGLVHAGRATQ (SEQ ID NO: 48) BPN', 242
TNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) ** * * * * ** **
** * * Protease C: 59.6% identity in 275 residues overlap; Score:
825.0; Gap frequency: 2.2% ProteaseC, 1
AQSVPWGISRVQAPAAHNRGLTGSGVRVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** * *** * ** * **** **** ** * * ** ProteaseC,
59 GNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSYSSIAQGLEWAGNNGMH
BPN', 61
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *************** ******** *** ** * * *** * * ProteaseC, 119
VASLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
*** ** ** * ** * ** **** ** * ** * **** ProteaseC, 175
DQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** **** *** ** * ****** ********* * * ProteaseC, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAAAR (SEQ ID NO: 49) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * *** Protease D: 59.3% identity in 275 residues overlap;
Score: 815.0; Gap frequency: 2.2% ProteaseD, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * ** * **** **** ** * * ** ProteaseD,
59 GNGHGTHVAGTIAALDNSIGVLGVAPSAELYAVKVLGASGSGAISSIAQGLEWAGNNGMH
BPN', 61
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *** *********** ******** *** * * * *** * * ProteaseD, 119
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** ProteaseD, 175
DQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** **** *** ** * ****** ********* * * ProteaseD, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 50) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * ** Protease E: 58.2% identity in 275 residues overlap;
Score: 800.0; Gap frequency: 2.2% ProteaseE, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * ** * **** **** ** * * ** ProteaseE,
59 GNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGGGAISSIAQGLEWAGNNGMH
BPN', 61
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *************** ******** * * * * * *** * * ProteaseE, 119
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----DSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** ProteaseE, 175
DQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAVLVKHKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** **** *** ** * ****** ******* * * * ProteaseE, 235
WSNVRIRDHLKKTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 51) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * * **
** ** * ** Protease A: 58.9% identity in 275 residues overlap;
Score: 812.0; Gap frequency: 2.2% Protease A, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * ** * **** **** ** * * ** Protease A,
59 GNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMH
BPN', 61
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *************** ******** *** * * * *** * * Protease A, 119
VANLSLGSPSAGGTLEQAVNSATSRGVLVVAASGNSGA----GSISAPASYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** * ** * ** **** ** * * * **** Protease A, 175
DQNNNRASFSQYGPGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** ** ** **** *** ** * ****** ********* * * Protease A, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 52) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * ** PD498: 47.7% identity in 266 residues overlap; Score:
487.0; Gap frequency: 4.9% PD498, 13
YGPQNTSTPAAWDVTRGSSTQTVAVLDSGVDYNHPDLARKVIKGYDFIDRDN-NPMDLNG BPN',
6 YGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDL--KVAGGASMVPSETPNFQDDNS **
** * *** *** * **** ** * * * * PD498, 72
HGTHVAGTVAADTNNGIGVAGMAPDTKILAVRVLDANGSGSLDSIASGIRYAADQGAKVL BPN',
64 HGTHVAGTVAA-LNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMDVI
*********** ** *** * ** ** ** *** * ** * * PD498, 132
NLSLGCECNSTTLKSAVDYAWNKGAVVVAAAGND----NVSRTFQPASYPNAIAVGAIDS BPN',
123 NMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAVDS *
*** * ** *** * * ******** * * ** ***** ** PD498, 188
NDRKASFSNYGTWVDVTAPGVNIASTVPNNGYSYMSGTSMASPHVAGLAALLASQGKN-- BPN',
183 SNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWT
**** * ** **** * ** * * * *********** *** * * PD498, 246
NVQIRQAIEQTADKISGTGTNFKYGK (SEQ ID NO: 53) BPN', 243
NTQVRSSLQNTTTKL---GDSFYYGK (SEQ ID NO: 46) * * * * * * * ***
Properase: 58.9% identity in 275 residues overlap; Score: 813.0;
Gap frequency: 2.2% Properase, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * ** * **** **** ** * * ** Properase,
59 GNGHGTHVAGTIAALNNSIGVLGVAPNAELYAVKVLGASGGGSNSSIAQGLEWAGNNGMH
BPN', 61
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** ************** ******** * * * * * *** * * Properase, 119
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** Properase, 175
DQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** **** *** ** * ****** ********* * * Properase, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 54) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * ** Relase: 60.7% identity in 275 residues overlap;
Score: 858.0; Gap frequency: 1.8% Relase, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGIDSTHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * **** **** **** ** * * ** Relase, 60
GNGHGTHVAGTIAALDNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMD BPN',
61 DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *** *********** ******** *** * * * *** * ** Relase, 120
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** Relase, 176
DQNNNRASFSQYGAELDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVLQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * *** **** *** ** * ****** ********* * * * Relase, 236
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 55) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * **
Savinase: 59.6% identity in 275 residues overlap; Score: 821.0; Gap
frequency: 2.2% Savinase, 1
AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGI-STHPDLNIRGGASFVPGE-PSTQD BPN',
1 AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
***** * * *** * * *** ***** * ** * **** **** ** * * ** Savinase, 59
GNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMH BPN',
61 DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD *
******** *************** ******** *** * * * *** * * Savinase, 119
VANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGA----GSISYPARYANAMAVGAT BPN',
121 VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
* *** ** ** * ** * ** **** ** * ** * **** Savinase, 175
DQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPS BPN',
181 DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN *
* ***** * ** **** *** ** * ****** ********* * * Savinase, 235
WSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR (SEQ ID NO: 56) BPN', 241
WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) * * * * * ** *
** ** ** * **
[0473] To find the homologous positions in subtilisin protease
sequences not shown in the alignment of Table 1A, the sequence of
interest is aligned to the sequence of BPN' as shown in Table 1B
for YaB protease and Subtilisin sendai. The new sequence is aligned
to the BPN' sequence by using the GAP alignment to the most
homologous sequence found by the GAP program. GAP is provided in
the GCG program package (Program Manual for the Wisconsin Package,
Version 8, August 1994, Genetics Computer Group, 575 Science Drive,
Madison, Wis., USA 53711) (Needleman, S. B. and Wunsch, C. D.,
(1970), Journal of Molecular Biology, 48, 443-45).
[0474] The sequence of the YaB protease is disclosed by Kaneko, R.;
Koyama, N.; Tsai, Y.-C.; Juang, R.-Y.; Yoda, K.; Yamasaki, M.;
Molecular cloning of the structural gene for alkaline elastase YaB,
a new subtilisin produced by an alkalophilic Bacillus strain. J.
Bacteriol. 171:5232 (1989), it has Swissprot number P20724, and is
shown in SEQ ID NO: 35.
[0475] The sequence of the Subtilisin sendai is disclosed by
Yamagata, Y.; Isshiki, K.; Ichishima, E.; Subtilisin Sendai from
alkalophilic Bacillus sp.: molecular and enzymatic properties of
the enzyme and molecular cloning and characterization of the gene,
aprS. Enzyme Microb. Technol. 17:653 (1995), it has SPTREMBL
accession number Q45522, and is shown in SEQ ID NO: 34.
Identity to savinase: 81.7% identity to savinase: 82.09%
Swissprot: P20724
TABLE-US-00003 [0476] TABLE 1B Alignment of YAB protease to BPN':
55.3% identity CLUSTAL W (1.7) multiple sequence alignment YAB
-QTVPWGINRVQAPIAQSRGFTGTGVRVAVLDTGISN-HADLRIRGGASFVPGE-PNISD
BPN{acute over ( )}
AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD
*:**:*:.:::** :*:*:**:.*:***:*:**.. *.**:: ****:**.* **:.* YAB
GNGHGTQVAGTIAALNNSIGVLGVAPNVDLYGVKVLGASGSGSISGIAQGLQWAANNGMH
BPN{acute over ( )}
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD
.*.***:****:**************. **.***** :***. * * :*::** *.*. YAB
IANMSLGSSAGSATMEQAVNQATASGVLVVAASGNSG----AGNVGFPARYANAMAVGAT
BPN{acute over ( )}
VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV :
*****..:***::: **::*.****:****:**.* :..**:*.:*...:****. YAB
DQNNNRATFSQYGAGLDIVAPGVGVQSTVPGNGYASFNGTSMATPHVAGVAALVKQKNPS
BPN{acute over ( )}
DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN
*..*:**:**. *. **::****.:***:*** *.::******:*****.***: .*:*. YAB
WSNVQIRNHLKNTATNLGNTTQFGSGLVNAEAATR (SEQ ID NO: 57) BPN{acute over
( )} WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) *:*.*:*.
*:**:*:**:: :*.**:*.:**:: Alignment of Subtilisin sendai to BPN':
55.6% identity. CLUSTAL W (1.7) multiple sequence alignment sendai
NQVTPWGITRVQAPTAWTRGYTGTGVRVAVLDTGIS-THPDLNIRGGVSFVPGE-PSYQD
BPN{acute over ( )}
AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETPNFQD *
.*:*:::::**: ::****:.*:***:*:**. :****:: **.*:**.* *.:** sendai
GNGHGTHVAGTIAALNNSIGVVGVAPNAELYAVKVLGANGSGSVSSIAQGLQWTAQNNIH
BPN{acute over ( )}
DNSHGTHVAGTVAALNNSIGVLGVAPSSALYAVKVLGDAGSGQYSWIINGIEWAIANNMD
.*.********:*********:****.: ******** ***. * * :*::*: **:. sendai
VANLSLGSPVGSQTLELAVNQATNAGVLVVAATGNNG----SGTVSYPARYANALAVGAT
BPN{acute over ( )}
VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGSTGSSSTVGYPGKYPSVIAVGAV *
*:***.* ** :*: **::*. :**:****:**:* *.**.**.:*...:****. sendai
DQNNNRASFSQYGTGLNIVAPGVGIQSTYPGNRYASLSGTSMATPHVAGVAALVKQKNPS
BPN{acute over ( )}
DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN
*..*:*****. *. *:::****.**** ***:*.: .*****:*****.***: .*:*. sendai
WSNTQIRQHLTSTATSLGNSNQFGSGLVNAEAATR (SEQ ID NO: 58) BPN{acute over
( )} WTNTQVRSSLQNTTTKLGDSFYYGKGLINVQAAAQ (SEQ ID NO: 46) *:***:*. *
.*:*.**:* :*.**:*.:**::
[0477] These alignments reveal that that homology between various
subtilisin proteases ranges between 100% and 40%.
[0478] Unless specified, subtilisin sequences and positions
mentioned in the present invention, are given in the BPN'
numeration, and can be converted by alignment as 50 described above
(Tables 1A and 1B).
[0479] Sequence identities between different pairs of proteases are
given below:
Sequence identity to BPN':
TABLE-US-00004 Savinase 60.4% Alcalase 69.5% BLAPR 60.4% ProteaseC
0.4% ProteaseD 0.0% ProteaseE 8.2% Protease A 0.0% Properase 9.6%
Relase 61.5% PD498 44.8% sendai 55.6% YAB 55.3%
Sequence identity to Savinase:
TABLE-US-00005 Alcalase 60.9% BLAPR 98.1% ProteaseC 8.5% ProteaseD
8.9% ProteaseE 6.7% Protease A 7.8% Properase 8.9% Relase 98.1%
PD498 44.3% sendai 81.4% YAB 81.8%
Structures
[0480] The protein structure of PD498 is disclosed in WO 98/35026
(Novo Nordisk). The structure of Savinase can be found in BETZEL et
al, J.MOL.BIOL., Vol. 223, p. 427, 1992 (1 svn.pdb).
Homology Modelling
[0481] Three dimensional structural models of the subtilisins
properase, release, ProteaseC, ProteaseD, ProteaseE, and PROTEASE B
were constructed based on three dimensional structure of Savinase
(Protein Data Bank entry 1SVN; Betzel, C., Klupsch, S., Papendorf,
G., Hastrup, S., Branner, S., Wilson, K. S.: Crystal structure of
the alkaline proteinase Savinase from Bacillus lentus at 1.4 .ANG.
resolution. J Mol Biol 223 pp. 427 (1992)) using the Modeller 5o (
Sali, A.; T. L. Blundell, "Definition of general topological
equivalence in protein structures: A procedure involving comparison
of properties and relationships through simulated annealing and
dynamic programming," J. Mol. Biol., 212 403-428 (1990)) module of
the Insight 2000 molecular modelling package (Biosym inc.). Default
parameters were used with the alignments shown in FIG. 1A as input,
e.g. alignment between the columns labelled Savinase and PROTEASE B
served as input alignment in construction of a PROTEASE B
structural model. The Modeller module by default output ten
structural models, of these the model with lowest `modeller
objective function` score was chosen as representing PROTEASE B
structure.
Lipase:
[0482] The sequence of the T. lanuginosus lipase (trade name
Lipolase) is provided in SEQ ID NO: 1 and the structure is
disclosed in WO 98/35026 and as "1tib", available in Structural
Classification of Proteins (SCOP) on the Internet.
Amylase:
[0483] The amylase used in the examples is the alpha-amylase of
Bacillus halmapalus (WO 96/23873), which is called amylase SP722
(the wild-type). Its sequence is shown in SEQ ID NO: 2 and the
corresponding protein structure was built from the BA2 structure,
as described in WO 96/23874. The first four amino acids of the
structural model are not defined, hence the sequence used for
numeration of amino acid residues in the examples of this invention
is four amino acids shorter than the one of the full length protein
SP722.
[0484] Several variants of this amylase are available (WO
96/23873). One particularly useful variant has deleted two amino
acid residues at D-G at positions 183 and 184 of the SEQ ID NO: 2
(corresponding to residues 179 and 180 of the modelled structure).
This variant is called JE-1 or Natalase.
[0485] Another amylase that is particularly useful is the amylase
AA560: This alkaline alpha-amylase may be derived from a strain of
Bacillus sp. DSM 12649. The strain was deposited on 25 Jan. 1999 by
the assignee under the terms of the Budapest Treaty on the
International Recognition of the Deposit of Microorganisms for the
Purposes of Patent Procedure at Deutshe Sammmlung von
Microorganismen and Zellkulturen GmbH (DSMZ), Mascheroder Weg 1b,
D-38124 Braunschweig DE.
Laccase:
[0486] The laccase used in this invention is that from Coprinus
cinereus (WO 98/38287), the sequence of which is shown as SEQ ID
NO: 3. The structure of the Myceliophthora thermophila laccase can
be built by homology modeling to the Coprinus cinereus laccase as
shown in WO 98/38287.
Cellulase:
[0487] The cellulase sequence and structure used in the present
invention is that of the core fragment of endoglucanase V from
Humicola insolens (aka Cel45 or Carezyme). The core fragment
structure is available as 3eng.pdb (G. J. DAVIES et al. ACTA
CRYSTALLOGR., SECT.D, Vol. 52, p. 7 1996; G. J. DAVIES et al.
BIOCHEMISTRY, V. 34, p. 16210, 1995); SwissProt accession number
P43316, and the sequences shown in SEQ ID 4. The corresponding
full-length sequence is disclosed in WO 91/17243 and shown here in
SEQ ID NO: 5. The numeration of all description and claims of this
invention pertain to the core fragment, however, it is contemplated
that all claims are also valid for the corresponding positions in
the full-length protein.
TABLE-US-00006 TABLE 1 Alignment and numeration scheme for
subtilisins (SEQ ID NOS: 46, 45, 47, 48, 49, 50, 51, 52, 54, 55,
56, 53, respectively) Pro- Pro- Pro- Pro- Pro- BPN' Alcalase teaseB
Esperase teaseC teaseD teaseE teaseA Properase Relase Savinase
PD498 -6 W -5 S -4 P -3 N -2 D -1 P 1 A A A A A A A A A A Y 2 Q Q Q
Q Q Q Q Q Q Q Q Y 3 S T T T S S S S S S S S 3a A 4 V V I V V V V V
V V V Y 5 P P P P P P P P P P P Q 6 Y Y W W W W W W W W W Y 7 G G G
G G G G G G G G G 8 V I I I I I I I I I I P 9 S P S S S S S S S S S
Q 10 Q L R F R R R R R R R N 11 I I V I V V V V V V V T 12 K K Q N
Q Q Q Q Q Q Q S 13 A A A T A A A A A A A T 14 P D P Q P P P P P P P
P 15 A K A Q A A A A A A A A 16 L V A A A A A A A A A A 17 H Q H H
H H H H H H H W 18 S A N N N N N N N N N D 19 Q Q R R R R R R R R R
V 20 G G G G G G G G G G G 21 Y F L I L L L L L L L T 22 T K T F T
T T T T T T R 23 G G G G G G G G G G G G 24 S A S N S S S S S S S S
25 N N G G G G G G G G G S 26 V V V A V V V V V V V T 27 K K K R R
K K K K K K Q 28 V V V V V V V V V V V T 28a V 29 A A A A A A A A A
A A A 30 V V V V V V V V V V V V 31 I L L L L L L L L L L L 32 D D
D D D D D D D D D D 33 S T T T T T T T T T T S 34 G G G G G G G G G
G G G 35 I I I I I I I I I I I V 36 D Q D D 37 S A S A S S S S S S
S Y 38 S S T S T T T T T T T N 39 H H H H H H H H H H H H 40 P P P
P P P P P P P P P 41 D D D D D D D D D D D D 42 L L L L L L L L L L
L L 43 K N N R N N N N N N N A 44 V V I I I I I I I I I R 44a K 44b
V 45 A V R A R R R R R R R I 46 G G G G G G G G G G G K 47 G G G G
G G G G G G G G 48 A A A A A A A A A A A Y 49 S S S S S S S S S S S
D 50 M F F F F F F F F F F F 51 V V V I V V V V V V V I 52 P A P S
P P P P P P P D 53 S G G S G G G G G G G R 54 E E E E E E E E E E E
D 55 T A P P P P P P P P N 56 P Y P N 57 N N S S S S S S S S S P 58
F T Y T T T T T T T M 59 Q T Q H Q Q Q Q Q Q Q 60 D D D D D D D D D
D D D 61 D G G N G G G G G G G L 62 N N N N N N N N N N N N 63 S G
G G G G G G G G G G 64 H H H H H H H H H H H H 65 G G G G G G G G G
G G G 66 T T T T T T T T T T T T 67 H H H H H H H H H H H H 68 V V
V V V V V V V V V V 69 A A A A A A A A A A A A 70 G G G G G G G G G
G G G 71 T T T T T T T T T T T T 72 V V I I I I I I I I I V 73 A A
A A A A A A A A A A 74 A A A A A A A A A A A A 75 L L L L L L L L L
L L D 75a T 76 N D N N N D N N N D N N 77 N N N N N N N N N N N N
78 S T S S S S S S S S S G 79 I T I I I I I I I I I I 80 G G G G G
G G G G G G G 81 V V V V V V V V V V V V 82 L L L L L L L L L L L A
83 G G G G G G G G G G G G 84 V V V V V V V V V V V M 85 A A A A A
A A A A A A A 86 P P P P P P P P P P P P 87 S S S S S S S S N S S D
88 S V A A A A A A A A A T 89 A S E D E E E E E E E K 90 L L L L L
L L L L L L I 91 Y Y Y Y Y Y Y Y Y Y Y L 92 A A A A A A A A A A A A
93 V V V V V V V V V V V V 94 K K K K K K K K K K K R 95 V V V V V
V V V V V V V 96 L L L L L L L L L L L L 97 G N G D G G G G G G G D
98 D S A R A A A A A A A A 99 A S S N S S S S S S S N 100 G G G G G
G G G G G G G 101 S S S S S S G S G S S S 102 G G G G G G G G G G G
G 103 Q S S S S A A S S S S S 104 Y Y V L Y I I V N V V L 105 S S S
A S S S S S S S D 106 W G S S S S S S S S S S 107 I I I V I I I I I
I I I 108 I V A A A A A A A A A A 109 N S Q Q Q Q Q Q Q Q Q S 110 G
G G G G G G G G G G G
111 I I L I L L L L L L L I 112 E E E E E E E E E E E R 113 W W W W
W W W W W W W Y 114 A A A A A A A A A A A A 115 I T G I G G G G G G
G A 116 A T N N N N N N N N N D 117 N N N N N N N N N N N Q 118 N G
G N G G G G G G G G 119 M M M M M M M M M M M A 120 D D H H H H H H
H D H K 121 V V V I V V V V V V V V 122 I I A I A A A A A A A L 123
N N N N S N N N N N N N 124 M M L M L L L L L L L L 125 S S S S S S
S S S S S S 126 L L L L L L L L L L L L 127 G G G G G G G G G G G G
128 G G S S S S S S S S S C 129 P A P T P P P P P P P E 130 S S S S
S S S S S S S C 131 G G P G P P P A P P P N 132 S S S S S S S G S S
S S 133 A T A S A A A G A A A T 134 A A T T T T T T T T T T 135 L M
L L L L L L L L L L 136 K K E E E E E E E E E K 137 A Q Q L Q Q Q Q
Q Q Q S 138 A A A A A A A A A A A A 139 V V V V V V V V V V V V 140
D D N N N N N N N N N D 141 K N S R S S S S S S S Y 142 A A A A A A
A A A A A A 143 V Y T N T T T T T T T W 144 A A S N S S S S S S S N
145 S R R A R R R R R R R K 146 G G G G G G G G G G G G 147 V V V I
V V V V V V V A 148 V V L L L L L L L L L V 149 V V V L V V V V V V
V V 150 V V V V V V V V V V V V 151 A A A G A A A A A A A A 152 A A
A A A A A A A A A A 153 A A S A S S S S S S S A 154 G G G G G G G G
G G G G 155 N N N N N N N N N N N N 156 E S S T S S S S S S S D 157
G G G G G G G G G G G N 158 S S A R A A A A A A A V 159 T S Q 160 G
G G G G G D G G G G S 161 S N S S S S S S S S R 162 S T I I I I I I
I I T 163 S N S S S S S S S S F 164 T T 165 V I V 166 G G N 167 Y Y
Y Y Y Y Y A Y Y Y Q 168 P P P P P P P P P P P P 169 G A A A A A A A
A A A A 170 K K R R R R R S R R R S 171 Y Y Y Y Y Y Y Y Y Y Y Y 172
P D A S A A A A A A A P 173 S S N G N N N N N N N N 174 V V A V A A
A A A A A A 175 I I M M M M M M M M M I 176 A A A A A A A A A A A A
177 V V V V V V V V V V V V 178 G G G A G G G G G G G G 179 A A A A
A A A A A A A A 180 V V T V T T T T T T T I 181 D D D D D D D D D D
D D 182 S S Q Q Q Q Q Q Q Q Q S 183 S N N N N N N N N N N N 184 N S
N G N N N N N N N D 185 Q N N Q N N N N N N N R 186 R R R R R R R R
R R R K 187 A A A A A A A A A A A A 188 S S S S S S S S S S S S 189
F F F F F F F F F F F F 190 S S S S S S S S S S S S 191 S S Q T Q Q
Q Q Q Q Q N 192 V V Y Y Y Y Y Y Y Y Y Y 193 G G G G G G G G G G G G
194 P A A P A A A P A A A T 195 E E G E G G G G G E G W 196 L L L I
L L L L L L L V 197 D E D E D D D D D D D D 198 V V I I I I I I I I
I V 199 M M M S V V V V V V V T 200 A A A A A A A A A A A A 201 P P
P P P P P P P P P P 202 G G G G G G G G G G G G 203 V A V V V V V V
V V V V 204 S G N N N N N N N N N N 205 I V I V V V V V V V V I 206
Q Y Q N Q Q Q Q Q Q Q A 207 S S S S S S S S S S S S 208 T T T T T T
T T T T T T 209 L Y Y Y Y Y Y Y Y Y Y V 210 P P P T P P P P P P P P
211 G T G G G G G G G G G N 212 N N S N S S S S S S S N 213 K T T R
T T T T T T T G 214 Y Y Y Y Y Y Y Y Y Y Y Y 215 G A A V A A A A A A
A S 216 A T S S S S S S S S S Y 217 Y L D L L L L L L L L M 218 N N
N S N N N N N N N S 219 G G G G G G G G G G G G 220 T T T T T T T T
T T T T 221 S S S S S S S S S S S S 222 M M M M M M M M M M M M 223
A A A A A A A A A A A A 224 S S T T T T T T T T T S 225 P P P P P P
P P P P P P 226 H H H H H H H H H H H H 227 V V V V V V V V V V V V
228 A A A A A A A A A A A A 229 G G G G G G G G G G G G 230 A A A V
A A A A A A A L 231 A A A A A A A A A A A A 232 A A A A A A V A A A
A A 233 L L L L L L L L L L L L 234 I I V V V V V V V V V L 235 L L
K K K K K K K L K A
236 S S Q S Q Q H Q Q Q Q S 237 K K K R K K K K K K K Q 238 H H N Y
N N N N N N N G 239 P P P P P P P P P P P K 240 N N S S S S S S S S
S N 241 W L W Y W W W W W W W 242 T S S T S S S S S S S 243 N A N N
N N N N N N N N 244 T S V N V V V V V V V V 245 Q Q Q Q Q Q R Q Q Q
Q Q 246 V V I I I I I I I I I I 247 R R R R R R R R R R R R 248 S N
N Q N N D N N N N Q 249 S R H R H H H H H H H A 250 L L L I L L L L
L L L I 251 Q S K N K K K K K K K E 252 N S N Q N N K N N N N Q 253
T T T T T T T T T T T T 254 T A A A A A A A A A A A 255 T T T T T T
T T T T T D 256 K Y S Y S S S S S S S K 257 L L L L L L L L L L L I
258 G G G G G G G G G G G S 259 D S S S S S S S S S S G 260 S S T P
T T T T T T T T 261 F F N S N N N N N N N G 262 Y Y L L L L L L L L
L T 263 Y Y Y Y Y Y Y Y Y Y Y N 264 G G G G G G G G G G G F 264a K
265 K K S N S S S S S S S Y 266 G G G G G G G G G G G G 267 L L L L
L L L L L L L K 268 I I V V V V V V V V V I 269 N N N H N N N N N N
N N 270 V V A A A A A A A A A S 271 Q E E G E E E E E E E N 272 A A
A R A A A A A A A K 273 A A A A A A A A A A A A 274 A A T T A T T T
T T T V 275 Q Q R Q R R R R R R R R 276 Y
EXAMPLES
Example 1
Identification of Epitope Sequences and Epitope Patterns
[0488] High diversity libraries (10.sup.12) of phages expressing
random hexa-, nona- or dodecapetides as part of their membrane
proteins, were screened for their capacity to bind purified
specific rabbit IgG, and purified rat and mouse IgG1 and IgE
antibodies. The phage libraries were obtained according to prior
art (se WO 9215679 hereby incorporated by reference).
[0489] The antibodies were raised in the respective animals by
subcutaneous, intradermal, or intratracheal injection of relevant
proteins (e.g. proteases, lipolytic enzymes, amylases,
oxidoreductases) dissolved in phosphate buffered saline (PBS). The
respective antibodies were purified from the serum of immunised
animals by affinity chromatography using paramagnetic immunobeads
(Dynal AS) loaded with pig anti-rabbit IgG, mouse anti-rat IgG1 or
IgE, or rat anti-mouse IgG1 or IgE antibodies.
[0490] The respective phage libraries were incubated with the IgG,
IgG1 and IgE antibody coated beads. Phages, which express
oligopeptides with affinity for rabbit IgG, or rat or mouse IgG1 or
IgE antibodies, were collected by exposing these paramagnetic beads
to a magnetic field. The collected phages were eluted from the
immobilised antibodies by mild acid treatment, or by elution with
intact enzyme. The isolated phages were amplified as know to the
specialist. Alternatively, immobilised phages were directly
incubated with E. coli for infection. In short, F-factor positive
E. coli (e.g. XL-1 Blue, JM101, TG1) were infected with M13-derived
vector in the presence of a helper-phage (e.g. M13K07), and
incubated, typically in 2xYT containing glucose or IPTG, and
appropriate antibiotics for selection. Finally, cells were removed
by centrifugation. This cycle of events was repeated 2-5 times on
the respective cell supernatants. After selection round 2, 3, 4,
and 5, a fraction of the infected E. coli was incubated on
selective 2xYT agar plates, and the specificity of the emerging
phages was assessed immunologically. Thus, phages were transferred
to a nitrocellulase (NC) membrane. For each plate, 2 NC-replicas
were made. One replica was incubated with the selection antibodies,
the other replica was incubated with the selection antibodies and
the immunogen used to obtain the antibodies as competitor. Those
plaques that were absent in the presence of immunogen, were
considered specific, and were amplified according to the procedure
described above.
[0491] The specific phage-clones were isolated from the cell
supernatant by centrifugation in the presence of polyethylenglycol.
DNA was isolated, the DNA sequence coding for the oligopeptide was
amplified by PCR, and the DNA sequence was determined, all
according to standard procedures. The amino acid sequence of the
corresponding oligopeptide was deduced from the DNA sequence.
[0492] Thus, a number of peptide sequences with specificity for the
protein specific antibodies, described above, were obtained. These
sequences were collected in a database, and analysed by sequence
alignment to identify epitope patterns. For this sequence
alignment, conservative substitutions (e.g. aspartate for
glutamate, lysine for arginine, serine for threonine) were
considered as one. This showed that most sequences were specific
for the protein the antibodies were raised against. However,
several cross-reacting sequences were obtained from phages that
went through 2 selection rounds only. In the first round 22 epitope
patterns were identified.
[0493] In further rounds of phage display, more antibody binding
sequences were obtained leading to more epitope patterns. Further,
the literature was searched for peptide sequences that have been
found to bind environmental allergen-specific antibodies (J All
Clin Immunol 93 (1994) pp. 34-43; Int Arch Appl Immunol 103 (1994)
pp. 357-364; Clin Exp Allergy 24 (1994) pp. 250-256; Mol Immunol 29
(1992) pp. 1383-1389; J Immunol 121 (1989) pp. 275-280; J. Immunol
147 (1991) pp. 205-211; Mol Immunol 29 (1992) pp. 739-749; Mol
Immunol 30 (1993) pp. 1511-1518; Mol Immunol 28 (1991) pp.
1225-1232; J. Immunol 151 (1993) pp. 7206-7213). These antibody
binding peptide sequences were included in the database.
[0494] A first generation database of antibody binding peptides
identified and their corresponding epitope patterns are shown in
Table 2-7 below.
Tables 2-7: Overview of the antibody binding peptide sequences,
epitope patterns and epitope sequences. The type of antibody used
for identifying the antibody binding sequences is indicated as IgG
or IgE and the species from which the antibodies were derived are
indicated as mo (mouse), ra (rat) and hu (human).
TABLE-US-00007 TABLE 2 Savinase antibody binding peptide sequences,
epitope patterns and epitope sequences. Antibody binding Method of
Epitope peptide identification pattern Donor Acceptor Epitope
Sequence (BPN') VQVYGDTSA (SEQ Phage Q > Y > D > Savinase
savinase Q206 V81 Y214 G80 D41 ID NO: 59) display T208 LQCVGS (SEQ
ID Protein a-amylase savinase L21 Q236 V26 G25 S24 NO: 60)
fragments inhibitor KRFANTELA (SEQ Phage R/K R F > N Savinase
savinase K251 R247 A174 N173 ID NO: 61) display LDQIFFTRW (SEQ
Phage D/E Q I F F T Savinase savinase L42/L75 D41 Q2 I79 ID NO: 62)
display FNDAFFVKM (SEQ Phage Savinase savinase N185 D181 A187 F189
ID NO: 63) display V203 ANIPIWSRSA (SEQ Phage > R S A Savinase
savinase R145 S144 A142 ID NO: 64) display ANIPIWSRSA (SEQ Phage
> R S A Savinase savinase S188 R186 S190 A179 ID NO: 64) display
RQSTDFGTT (SEQ Phage R Q > > Savinase savinase R186 Q191 S156
ID NO: 65) display D/E VQVYGDTSA (SEQ Phage Q > Y > D >
Savinase savinase Q191 Y192 ID NO: 66) display G193/A194/G195 D197
S265 RRFSNATRA (SEQ Phage R/K R F > N Savinase savinase K251
R247 A174 N173 ID NO: 67) display CTARLRAGNACG Phage A R > A
Savinase savinase A172/A169 R170 A194 (SEQ ID NO: 68) display G193
N261 LDQIFFTRW (SEQ Phage D/E Q I F F T Savinase savinase D60 Q59
I44/I35 ID NO: 69) display LDQIFFTRW (SEQ Phage D/E Q I F F T
Savinase savinase L42/L75 D41 Q2 I79 ID NO: 69) display EQIFFTSGL
(SEQ ID Phage D/E Q I F F T Savinase savinase E112 Q109 I79 NO: 70)
display GRFSNSKFK (SEQ Phage L > G R S Savinase savinase L196
G195 R170 S163 ID NO: 71) display AVLRDC (SEQ ID Protein a-amylase
savinase A254 V268 L267 R10 D181 NO: 72) fragments inhibitor LQCVGS
(SEQ ID Protein a-amylase savinase L217 Q206 V81 G80 S3 NO: 73)
fragments inhibitor LRQCNERCV (SEQ Phage R Q > > Savinase
savinase L267 R10 Q12 N269 E271 ID NO: 74) display D/E R275
SPVTKRASLKIDSKK Protein Der p II savinase A88 S87/T22 L233 K235
(SEQ ID NO: 75) fragments I246 RQSTDFGTT (SEQ Phage R Q > >
Savinase savinase R247 Q245 S240/S242 ID NO: 76) display D/E
FCTNNCELS (SEQ Phage N > > E L Savinase savinase T143 N173
N140 E136 L135 ID NO: 77) display FCTNNCELS (SEQ Phage N > >
E L Savinase savinase N117 N116 E112 L111 ID NO: 77) display
DFHVKYAAQ (SEQ Phage Savinase savinase ID NO: 78) display
VAQYKALPVVLENA Protein Fel d I savinase L135 P168 V139 L111 E112
(SEQ ID NO: 79) fragments N116 AAYPDV (SEQ ID Protein A > >
> > Y a-amylase savinase A215 Y214 P40 D41 V81 NO: 80)
fragments P > inhibitor EQIFFTSGL (SEQ ID Phage D/E Q I F F T
Savinase savinase E271 Q12 I8 NO: 81) display VDAAF (SEQ ID NO:
Protein Poa p IX savinase V203 D181 A179 A187 F189 82) fragments
AVLRDC (SEQ ID Protein a-amylase savinase A232 V234 L250 R247 D197
NO: 83) fragments inhibitor RAFRRNANW (SEQ Phage A R > A
Savinase savinase A272/A273 R275 R19 N18 ID NO: 84) display A15/A16
CTARLRAGNACG Phage A R > A Savinase savinase A15/A16 R19 L21
R275 (SEQ ID NO: 85) display A272 A273 N269 TFHDAPALQ (SEQ Phage
Savinase savinase H39 D41 A74/A73 P86 A88 ID NO: 86) display L90
CTARVVALGVCG Phage A R > A Savinase savinase R145 V147 V149 A151
(SEQ ID NO: 87) display L124/L126 G127 GRFSNSKFK (SEQ Phage L >
G R S Savinase savinase L148 G146 R145 ID NO: 88) display S144/S141
N140 RRFANDHTR (SEQ Phage R/K R F > N savinase savinase K27 R45
N43 D41 H39 ID NO: 89) display T38/T213 KRFANTEPA (SEQ Phage R/K R
F > N savinase savinase K251 R247 A174 N173 ID NO: 90) display
YKVSAL (SEQ ID Protein a-amylase savinase Y91 K27 V26 S24 G23 L21
NO: 91) fragments inhibitor TGKYVS (SEQ ID Protein a-amylase
savinase S24 G25 K27 Y91 V93 NO: 92) fragments inhibitor Antibody
binding peptide Epitope # IgG IgE VQVYGDTSA (SEQ sav1.1 Ra ID NO:
59) LQCVGS (SEQ ID sav19.1 Hu NO: 60) KRFANTELA (SEQ sav6.1 Ra- Mo
ID NO: 61) Mo LDQIFFTRW (SEQ sav5.1 Ra ID NO: 62) FNDAFFVKM (SEQ
sav11.0 Ra ID NO: 63) ANIPIWSRSA (SEQ sav3.2-lac1.0- Ra ID NO: 64)
lip4.0-pd5.0 ANIPIWSRSA (SEQ sav3.1-lac1.0- Ra ID NO: 64)
lip4.0-pd5.0 RQSTDFGTT (SEQ sav2.2 Ra ID NO: 65) VQVYGDTSA (SEQ
sav1.2 Ra ID NO: 66) RRFSNATRA (SEQ sav6.1 Ra- Mo ID NO: 67) Mo
CTARLRAGNACG sav10.4 Ra (SEQ ID NO: 68) LDQIFFTRW (SEQ sav5.2 Ra ID
NO: 69) LDQIFFTRW (SEQ sav5.1 Ra ID NO: 69) EQIFFTSGL (SEQ ID
sav5.4 Ra NO: 70) GRFSNSKFK (SEQ sav9.2-je4.0-lip5.1-5.2 Ra ID NO:
71) AVLRDC (SEQ ID sav18.1-pd18.1-18.2 Hu NO: 72) LQCVGS (SEQ ID
sav19.2 Hu NO: 73) LRQCNERCV (SEQ sav2.1 Ra ID NO: 74)
SPVTKRASLKIDSKK sav16.0-pd7.0 Hu (SEQ ID NO: 75) RQSTDFGTT (SEQ
sav2.3 Ra ID NO: 76) FCTNNCELS (SEQ sav7.2 Ra ID NO: 77) FCTNNCELS
(SEQ sav7.1 Ra ID NO: 77) DFHVKYAAQ (SEQ sav8.0 Ra ID NO: 78)
VAQYKALPVVLENA sav12.0-pd8.0 Hu (SEQ ID NO: 79) AAYPDV (SEQ ID
sav13.0-pd13.1-13.2 Hu NO: 80) EQIFFTSGL (SEQ ID sav5.3 Ra NO: 81)
VDAAF (SEQ ID NO: sav15.0-pd12.0 Hu 82) AVLRDC (SEQ ID
sav18.2-pd18.1-18.2 Hu NO: 83) RAFRRNANW (SEQ sav10.1 Ra ID NO: 84)
CTARLRAGNACG sav10.2 Ra (SEQ ID NO: 85) TFHDAPALQ (SEQ sav4.0 Ra ID
NO: 86) CTARVVALGVCG sav10.3 Ra (SEQ ID NO: 87) GRFSNSKFK (SEQ
sav9.1-je4.0-lip5.1-5.2 Ra ID NO: 88) RRFANDHTR (SEQ sav6.2 Ra ID
NO: 89) KRFANTEPA (SEQ sav6.1 Ra- Mo ID NO: 90) Mo YKVSAL (SEQ ID
sav14.0-pd14.0 Hu NO: 91) TGKYVS (SEQ ID sav17.0-pd17.1-17.2 Hu NO:
92)
TABLE-US-00008 TABLE 3 PD498 antibody binding peptide sequences,
epitope patterns and epitope sequences. Epitope pattern Donor
Acceptor Epitope Sequence (BPN') Epitope # IgG IgE Fel d I pd498
V198 A254 Q252 Y276 K239 A235 L233 P86 pd8.0 Hu A > > >
a-amylase pd498 *3aA Y1/Y2 P-4/P-1 D-2 V81 pd13.2 Hu > Y P >
inhibitor Poa p IX pd498 S182 Y6 G7 P8 T13 P14 A15 A16 pd11.0 Hu
> K L > > Poa p IX pd498 Y171 K136 L135 A108 Y113 pd4.4 Hu
a-amylase pd498 Y48/Y37 K46 *44aaV A43 L42 pd14.0 Hu inhibitor Poa
p IX pd498 V196/V198 D197 A174/A176 A169 F163 pd12.0 Hu K Q S Poa p
IX pd498 A142 A147 V148 K120 Q27 S24/S25 pd2.3 Hu K Q S pd498 pd498
R44 K89 Q27 S236 K120 G146 pd2.2 Ra Der p II pd498 *28aV T88 *44a K
R44 A43 L42 pd7.0 Hu > K L > > pd498 pd498 N56/N55 K46 L91
A29/A119 T28 pd4.3 Ra > K L > > pd498 pd498 N240/N243 K239
L233/L234 A16 T21 R22 pd4.1 Ra > K L > > Poa p IX pd498
Y37 K46 L91 A114 Y113 pd4.5 Hu > K L > > pd498 pd498
N240/N243 K239 L233/L234 A16 T21 R22 pd4.1 Ra Y I > K L pd498
pd498 Y113 I111 A108/A138 K136 L135 pd3.1 Ra K Q S pd498 pd498 A115
K145 N243 N240 K239 Q237 S236 pd2.1 Ra > R Y > K/R pd498
pd498 R94 R53 Y48 Q117 R112 S109/S137 pd1.5-lac2.0 Ra Phl p V pd498
A169 Q167 F163 T162 S160 G193 pd10.0 Hu Y I > K L pd498 pd498
Y276 I246 K239 L234 S236 pd3.2 Ra > K L > > pd498 pd498
N240/N243 K239 L233/L234 R22 P86 pd4.2 Ra A > > >
a-amylase pd498 *3aA Y2 P14 D18 V19 pd13.1 Hu > Y P >
inhibitor K Q S Poa p IX pd498 A15 A16 V274 K239 Q237 S236 pd2.4 Hu
a-amylase pd498 G146 K145 Y141 V139 S137 pd17.2 Hu inhibitor
a-amylase pd498 A273 V274 L233 R22 D87 pd18.1 Hu inhibitor A R >
A Par j 1 + pd498 N10 S12 A15/A16 R275 A273/A249 R247 pd9.0 Hu + Ra
Hu Par o 1 A174 D197 S170 pd498 pd498 R22 G23 L233 S236 pd6.2 Ra
> R Y > K/R pd498 pd498 R94 R53 Y48 P57 K46 L91 pd1.4-lac2.0
Ra > R Y > K/R pd498 pd498 R94 R53 Y48 P57 K46 L91
pd1.4-lac2.0 Ra a-amylase pd498 L96 R94 S33 V35 Y37 pd15.0 Hu
inhibitor > R Y > K/R pd498 pd498 S109/S137 R112 Y141 N144
K145 pd1.3-lac2.0 Ra > R Y > K/R pd498 pd498 T162 R161 Y192
N191 K186 pd1.2-lac2.0 Ra > R Y > K/R pd498 pd498 T133/T134
R112 Y141 N144 K145 pd1.1-lac2.0 Ra a-amylase pd498 A92 *44aaV L42
R44 D75 pd18.2 Hu inhibitor a-amylase pd498 S236 G238 K239 Y276
V274 S270 pd17.1 Hu inhibitor a-amylase pd498 S12 P14 W17 S-5 W-6
pd16.0 Hu inhibitor > R S A pd498 pd498 S137 R112 S109 A108
pd5.0-lac1.0-lip4.0- Ra sav3.1-3.2 pd498 pd498 S215 M217 I205 M222
G219 pd6.1 Ra
TABLE-US-00009 TABLE 4 Antibody binding peptide sequences, epitope
patterns and epitope sequences for the T. lanuginosus lipase
(Lipolase). Antibody binding Method of Epitope peptide
identification pattern Donor Acceptor Epitope Sequence Epitope #
IgG IgE QRPPRYELE (SEQ Phage R P P R lipolase lipolase lip1.0 Ra ID
NO: 93) display ELEYRPPRQ (SEQ Phage > E Y lipolase lipolase
L124 E129 Y164 lip2.1 Ra ID NO: 94) display HEYDMRVAW (SEQ Phage
> E Y lipolase lipolase H215 E219 Y220 lip2.2 Ra ID NO: 95)
display HEYPMDFHL (SEQ Phage > E Y lipolase lipolase H215 E219
Y220 lip2.2 Ra ID NO: 96) display SEYSMSITP (SEQ Phage > E Y
lipolase lipolase S217 E219 Y220 lip2.3 Ra ID NO: 97) display
CVWPAHAPLSCG Phage > P > > P lipolase lipolase P253 P250
A243 lip3.0 Ra (SEQ ID NO: 98) display A P > S P208/P207
S214/S216/S217 CSWPSPAPLSCG Phage > P > > P lipolase
lipolase P253 P250 A243 lip3.0 Ra (SEQ ID NO: 99) display A P >
S P208/P207 S214/S216/S217 CDFPLHAPLSCG Phage > P > > P
lipolase lipolase P253 P250 A243 lip3.0 Ra (SEQ ID NO: 100) display
A P > S P208/P207 S214/S216/S217 CLFPSPAPRSCG Phage > P >
> P lipolase lipolase P253 P250 A243 lip3.0 Ra (SEQ ID NO: 101)
display A P > S P208/P207 S214/S216/S217 CDGPAPAPWSCG Phage >
P > > P lipolase lipolase P253 P250 A243 lip3.0 Ra (SEQ ID
NO: 102) display A P > S P208/P207 S214/S216/S217 CSFPLPAPRSCG
Phage > P > > P lipolase lipolase P253 P250 A243 lip3.0 Ra
(SEQ ID NO: 103) display A P > S P208/P207 S214/S216/S217
CVYPSPAPWSCG Phage > P > > P lipolase lipolase P253 P250
A243 lip3.0 Ra (EQ ID NO: 104) display A P > S P208/P207
S214/S216/S217 PEYTMNALS (SEQ Phage > E Y lipolase lipolase P218
E219 Y220 lip2.4 Ra ID NO: 105) display CSRSAKGARLCG Phage > R S
A lipolase lipolase R209 S214 A182 lip4.0-lac1.0- Ra (EQ ID NO:
106) display pd5.0- sav3.1-3.2 LEYPMSASQ (SEQ Phage > E Y
lipolase lipolase L124 E129 Y164 lip2.1 Ra ID NO: 107) display
RKLTLSGRS (SEQ Phage L > G R S lipolase lipolase L67 G65 R81
S83/S85 lip5.1-je4.0- Ra ID NO: 108) display sav9.0 RKLTLSGRS (SEQ
Phage L > G R S lipolase lipolase L96/L97 G212 R209/
lip5.2-je4.0- Ra ID NO: 109) display R179 S214 sav9.0 SYGAPATPAA
(SEQ Protein Poa p IX lipolase S170 Y171 G172 A173 lip6.0 Hu ID NO:
110) fragments P174 A150 T153 PAAGYTPAAP (SEQ Protein Poa p IX
lipolase A18/A19/A20 G65 Y53 lip7.0 Hu Hu ID NO: 111) fragments
T123 YKLAY (SEQ ID NO: Protein Poa p IX lipolase Y138 K74 L75 A68
Y16 lip8.1 Hu 112) fragments YKLAY (SEQ ID NO: Protein Poa p IX
lipolase Y53 K127 L67 A68 Y16 lip8.2 Hu 112) fragments KYDDYVATLS
(SEQ Protein Poa p IX lipolase Y194 D167 D165 Y164 lip9.0 Hu ID NO:
113) fragments V132 A131 L52 S54 EVKATPAGEL (SEQ Protein Poa p IX
lipolase E43 V44 K46 A47 T72 lip10.0 Hu ID NO: 114) fragments
CGYSNAQGVDYWI Protein Der p I lipolase Y53 S54 N25/N26 lip15.0 Hu
Hu (SEQ ID NO: 115) fragments A18/A19/A20 Q15 V44 VPGIDPNACHYMKC
Protein Der p II lipolase P256 I255 D254 P253 lip16.0 Hu (SEQ ID
NO: 116) fragments N200 H198 Y261 SPVTKRASLKIDSKK Protein Der p II
lipolase R179 A182 S216/S217 lip17.0 Hu (SEQ ID NO: 117) fragments
I238 K237 I235 D234 S224 K223 IMSALAMVYLGAK Protein Ovalbumin
lipolase V140 Y138 L69 A49 A47 lip18.0 Hu (SEQ ID NO: 118)
fragments K46 ELGVRE (SEQ ID Protein a-amylase lipolase E99 L97
G109/G177 lip11.0 Hu NO: 119) fragments inhibitor V176 R175 D242
GCRKEV (SEQ ID Protein a-amylase lipolase G106 C107 R108 K98
lip12.0 Hu NO: 120) fragments inhibitor E99 LRSVYQ (SEQ ID Protein
a-amylase lipolase L147 R81 S79 V77 Y16 lip13.0 Hu NO: 121)
fragments inhibitor Q15 SGPWSW (SEQ ID Protein a-amylase lipolase
S170 G172 P174 W89 lip14.0 Hu NO: 122) fragments inhibitor S83
TABLE-US-00010 TABLE 5 Amylase (Natalase) antibody binding peptide
sequences, epitope patterns and epitope sequences. Antibody binding
Method of peptide identification Epitope pattern Donor Acceptor
Epitope Sequence ARIDPRGPS (SEQ Phage A > I D P R/K amylase
amylase A380 K381 I382 D383 P384 ID NO: 123) display R389 ARIDPRHGS
(SEQ Phage A > I D P R/K amylase amylase A380 K381 1382 D383
P384 ID NO: 124) display R389 CSVAKIDPRTCG Phage A > I D P R/K
amylase amylase A109 K138 D140 P142 (SEQ ID NO: 125) display R144
CSVAKIDPRTCG Phage A > 1 D P R/K amylase amylase A380 K381 I382
D383 P384 (SEQ ID NO: 125) display R389 AKIDPKPDT (SEQ Phage A >
I D P R/K amylase amylase A109 K138 D140 P142 ID NO: 126) display
R144 AKIDPKPDT (SEQ Phage A > I D P R/K amylase amylase A380
K381 I382 D383 P384 ID NO: 126) display R389 ARIDPRHGS (SEQ Phage A
> I D P R/K amylase amylase A109 K138 D140 P142 ID NO: 127)
display R144 QIYNDTGPT (SEQ Phage Q > Y > D > amylase
amylase Q390 L386 Y368/Y367 D366 ID NO: 128) display QIYNDTGPT (SEQ
Phage Q > Y > D > amylase amylase Q170 I173 Y196 D195 ID
NO: 128) display QIYNDTGPT (SEQ Phage Q > Y > D > amylase
amylase Q357 I352 Y349 D366 ID NO: 128) display QIYNDTGPT (SEQ
Phage Q > Y > D > amylase amylase Q331 I370 Y368/Y367 D366
ID NO: 128) display CGSATIDPRQCG Phage A > I D P R/K amylase
amylase A109 K138 D140 P142 (SEQ ID NO: 129) display R144
CNADNQMYPQCG Phage A > > > > Y P > amylase amylase
N29 A27 D26/D25 Y8 (SEQ ID NO: 130) display P41/P42 ARIDPRGPS (SEQ
Phage A > I D P R/K amylase amylase A109 K138 D140 P142 ID NO:
131) display R144 CGSATIDPRQCG Phage A > I D P R/K amylase
amylase A380 K381 I382 D383 P384 (SEQ ID NO: 132) display R389
CDADSSGYPLCG Phage A > > > > Y P > amylase amylase
A107/A109 D108 Y57 (SEQ ID NO: 133) display P41/42 QLYGDEQLP (SEQ
Phage Q > Y > D > amylase amylase Q331 I370 Y368/Y367 D366
ID NO: 134) display QLYGDEQLP (SEQ Phage Q > Y > D >
amylase amylase Q357 I352 Y349 D366 ID NO: 134) display QLYGDEQLP
(SEQ Phage Q > Y > D > amylase amylase Q170 I173 Y196 D195
ID NO: 134) display QLYGDEQLP (SEQ Phage Q > Y > D >
amylase amylase Q390 L386 Y368/Y367 D366 ID NO: 134) display
RYAQIDPRW (SEQ Phage A > I D P R/K amylase amylase A380 K381
I382 D383 P384 ID NO: 135) display R389 RYAQIDPRW (SEQ Phage A >
I D P R/K amylase amylase A109 K138 D140 P142 ID NO: 135) display
R144 GEFNLGRSS (SEQ Phage L > G R S amylase amylase L88 G92 R31
S28 ID NO: 136) display CNADSWGYPRCG Phage A > > > > Y
P > amylase amylase N29 A27 D26/D25 Y8 (SEQ ID NO: 137) display
P41/P42 CNADNQMYPQCG Phage A > > > > Y P > amylase
amylase N102 A233 D232 Y54 (SEQ ID NO: 138) display P41/P42
CNADSWGYPRCG Phage A > > > > Y P > amylase amylase
N102 A233 D232 Y54 (SEQ ID NO: 137) display P41/P42 GEFNLGRSS (SEQ
Phage L > G R S amylase amylase L62 G63/G76 R78 S79 ID NO: 139)
display Antibody binding peptide Epitope # IgG IgE ARIDPRGPS (SEQ
je1.1 Ra ID NO: 123) ARIDPRHGS (SEQ je1.1 Ra ID NO: 124)
CSVAKIDPRTCG je1.2 Ra (SEQ ID NO: 125) CSVAKIDPRTCG je1.1 Ra (SEQ
ID NO: 125) AKIDPKPDT (SEQ je1.2 Ra ID NO: 126) AKIDPKPDT (SEQ
je1.1 Ra ID NO: 126) ARIDPRHGS (SEQ je1.2 Ra ID NO: 127) QIYNDTGPT
(SEQ je2.4 Ra ID NO: 128) QIYNDTGPT (SEQ je2.3 Ra ID NO: 128)
QIYNDTGPT (SEQ je2.2 Ra ID NO: 128) QIYNDTGPT (SEQ je2.1 Ra ID NO:
128) CGSATIDPRQCG je1.2 Ra (SEQ ID NO: 129) CNADNQMYPQCG je3.1 Ra
(SEQ ID NO: 130) ARIDPRGPS (SEQ je1.2 Ra ID NO: 131) CGSATIDPRQCG
je1.1 Ra (SEQ ID NO: 132) CDADSSGYPLCG e3.3 Ra (SEQ ID NO: 133)
QLYGDEQLP (SEQ je2.1 Ra ID NO: 134) QLYGDEQLP (SEQ je2.2 Ra ID NO:
134) QLYGDEQLP (SEQ je2.3 Ra ID NO: 134) QLYGDEQLP (SEQ je2.4 Ra ID
NO: 134) RYAQIDPRW (SEQ je1.1 Ra ID NO: 135) RYAQIDPRW (SEQ je1.2
Ra ID NO: 135) GEFNLGRSS (SEQ je4.1-sav9.0-lip5.1-5.2 Ra ID NO:
136) CNADSWGYPRCG je3.1 Ra (SEQ ID NO: 137) CNADNQMYPQCG je3.2 Ra
(SEQ ID NO: 138) CNADSWGYPRCG je3.2 Ra (SEQ ID NO: 137) GEFNLGRSS
(SEQ je4.2-sav9.0-lip5.1-5.2 Ra ID NO: 139)
TABLE-US-00011 TABLE 6 Cellulase (Carezyme; Cel45 from Humicola
insolens) antibody binding peptide sequences, epitope patterns and
epitope sequences. Antibody binding Method of peptide
identification Epitope pattern Donor Acceptor Epitope Sequence
Epitope # IgG IgE CVHAGPRAGTCG Phage display > G > > A G
carezyme carezyme P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 140)
CVHAGPRAGTCG Phage display V H > G > carezyme carezyme car2.0
Ra (SEQ ID NO: 140) CLSGPLAGRVCG Phage display > G > > A G
carezyme carezyme P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 141)
CRISPWYSVPCG Phage display carezyme carezyme car3.0 Ra (SEQ ID NO:
142) CLSGPAAGQSCG Phage display > G > > A G carezyme
carezyme P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 143) Phage display
A > I D P R/K je-1 carezyme R146 I131 D133 car11.2 Ra P137
CITRGTRAGWCG Phage display > G > > A G carezyme carezyme
P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 144) Phage display A R >
A savinase carezyme A191 R200 R201 car6.2 Ra A83 N81 CLSGPAAGQSCG
Phage display > G > > A G carezyme carezyme T95/S96 G27
P98 car1.2 Ra (SEQ ID NO: 143) A100 G101 Phage display A > I D P
R/K je-1 carezyme A195 R37 I38 D40 car11.1 Ra L44 Phage display Q
> Y > D > savinase, carezyme Q59 Y54 G134 D133 car10.0 Ra
T136 je-1 Phage display > P > > A P > S lipoprime
carezyme W62/W169 P61 P165 car9.0 Ra A162 P160 CITRGTRAGWCG Phage
display > G > > A G carezyme carezyme T95/S96 G27 P98
car1.2 Ra (SEQ ID NO: 144) A100 G101 Phage display R/K R F > N
savinase carezyme R7 R170 F174 car7.0 Ra A177 CLSGPLAGRVCG Phage
display > G > > A G carezyme carezyme T95/S96 G27 P98
car1.2 Ra (SEQ ID NO: 145) A100 G101 Phage display A R > A
savinase carezyme A1 R4 R7 A177 car6.1 Ra N176 Phage display > P
> R D T G laccase carezyme D178 P180 R4 D2 car5.0 Ra S183 Phage
display > R Y > K/R pd498 carezyme R170 R153 Y168 P165 car4.0
Ra K164 L163 Phage display D/E Q I F F T savinase carezyme Q36 I38
F41 F29 car8.0 Ra T197 CLTAGPSAGYCG Phage display > G > >
A G carezyme carezyme T95/S96 G27 P98 car1.2 Ra (SEQ ID NO: 146)
A100 G101 CYTTGRLAGLCG Phage display > G > > A G carezyme
carezyme P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 147) CYTTGRLAGLCG
Phage display > G > > A G carezyme carezyme T95/S96 G27
P98 car1.2 Ra (SEQ ID NO: 147) A100 G101 CVHSGPRAGYCG Phage display
> G > > A G carezyme carezyme P23 R201 A83 G84 car1.1 Ra
(SEQ ID NO: 148) CVHSGPRAGYCG Phage display V H > G >
carezyme carezyme car2.0 Ra (SEQ ID NO: 148) CVHAGPRAGTCG Phage
display > G > > A G carezyme carezyme T95/S96 G27 P98
car1.2 Ra (SEQ ID NO: 149) A100 G101 CVHSGPRAGYCG Phage display
> G > > A G carezyme carezyme T95/S96 G27 P98 car1.2 Ra
(SEQ ID NO: 148) A100 G101 CVHSGLSRRLLR Phage display V H > G
> carezyme carezyme car2.0 Ra (SEQ ID NO: 150) CVTRGPNAGSCG
Phage display > G > > A G carezyme carezyme T95/S96 G27
P98 car1.2 Ra (SEQ ID NO: 151) A100 G101 CLTAGPSAGYCG Phage display
> G > > A G carezyme carezyme P23 R201 A83 G84 car1.1 Ra
(SEQ ID NO: 152) CVTRGPNAGSCG Phage display > G > > A G
carezyme carezyme P23 R201 A83 G84 car1.1 Ra (SEQ ID NO: 151)
CITSGPRAGNCG Phage display > G > > A G carezyme carezyme
T95/S96 G27 P98 car1.2 Ra (SEQ ID NO: 153) A100 G101 CITSGPRAGNCG
Phage display > G > > A G carezyme carezyme P23 R201 A83
G84 car1.1 Ra (SEQ ID NO: 153)
TABLE-US-00012 TABLE 7 Laccase (Myceliophthora thermopila laccase)
antibody binding peptide sequences, epitope patterns and epitope
sequences. Antibody binding Method of Epitope peptide
identification pattern Donor Acceptor Epitope Sequence Epitope #
IgG IgE PQSD5PGESQ (SEQ Phage display P > S/T D P G laccase
laccase P180 R175 T168 D166 lac3.2 Ra ID NO: 154) P165 G265
WPKSDAGDS (SEQ ID Phage display P > > D A G laccase laccase
P241 R409 S410/S416 D434 lac4.1 Ra NO: 155) A389 G390 PQSDAGVVM
(SEQ ID Phage display P > > D A G laccase laccase P241 R409
S410/S416 D434 lac4.1 Ra NO: 156) A389 G390 DPVRDTGAG (SEQ ID Phage
display > P > R D T G laccase laccase P241 R409 D434 T432
lac5.1 Ra NO: 157) G430/G390 GPSRDAGLL (SEQ ID Phage display P >
> D A G laccase laccase P241 R409 S410/S416 D434 lac4.1 Ra NO:
158) A389 G390 PASDAGRGP (SEQ ID Phage display P > > D A G
laccase laccase P241 R409 S410/S416 D434 lac4.1 Ra NO: 159) A389
G390 PRDSTGLAL (SEQ ID Phage display P > S/T D P G laccase
laccase P378 R379 T442 D443 lac3.1 Ra NO: 160) P445 G446 PQSDPGESQ
(SEQ ID Phage display P > S/T D P G laccase laccase P378 R379
T442 D443 lac3.1 Ra NO: 161) P445 G446 RYPFLRATN (SEQ ID Phage
display > R Y > K/R laccase laccase lac2.0- Ra NO: 162)
pd1.1-1.4 GAARDARSA (SEQ ID Phage display > R S A laccase
laccase lac1.0- Ra NO: 163) lip4.0- pd5.0- sav3.1- 3.2 PRSDTGFGS
(SEQ ID Phage display > P > R D T G laccase laccase P241 R409
D434 T432 lac5.1 Ra NO: 164) G430/G390 LPRSDPGGR (SEQ ID Phage
display P > S/T D P G laccase laccase P180 R175 T168 D166 lac3.2
Ra NO: 165) P165 G265 DPARDTGDV (SEQ ID Phage display > P > R
D T G laccase laccase P241 R409 D434 T432 lac5.1 Ra NO: 166)
G430/G390 APKSDNGIT (SEQ ID Phage display P > > D A G laccase
laccase P241 R409 S410/S416 lac4.1 Ra NO: 167) D434 A389 G390
PKSDPGTNW (SEQ ID Phage display P > S/T D P G laccase laccase
P378 R379 T442 D443 lac3.1 Ra NO: 168) P445 G446 PRTDPGWLA (SEQ ID
Phage display P > S/T D P G laccase laccase P378 R379 T442 D443
lac3.1 Ra NO: 169) P445 G446 LPRSDPGGR (SEQ ID Phage display P >
S/T D P G laccase laccase P378 R379 T442 D443 lac3.1 Ra NO: 170)
P445 G446 PSSDPGARS (SEQ ID Phage display P > S/T D P G laccase
laccase P180 R175 T168 D166 lac3.2 Ra NO: 171) P165 G265 HVFDKNVTR
(SEQ ID Phage display laccase laccase lac6.0 NO: 172) PRSDPGTPT
(SEQ ID Phage display P > S/T D P G laccase laccase P378 R379
T442 D443 lac3.1 Ra NO: 173) P445 G446 PRSDPGTPT (SEQ ID Phage
display P > S/T D P G laccase laccase P180 R175 T168 D166 lac3.2
Ra NO: 173) P165 G265 PRDSTGLAL (SEQ ID Phage display P > S/T D
P G laccase laccase P180 R175 T168 D166 lac3.2 Ra NO: 174) P165
G265 PRTDPGWLA (SEQ ID Phage display P > S/T D P G laccase
laccase P180 R175 T168 D166 lac3.2 Ra NO: 175) P165 G265 PSSDPGARS
(SEQ ID Phage display P > S/T D P G laccase laccase P378 R379
T442 D443 lac3.1 Ra NO: 176) P445 G446 PKSDPGTNW (SEQ ID Phage
display P > S/T D P G laccase laccase P180 R175 T168 D166 lac3.2
Ra NO: 177) P165 G265 WPKSDAGDS (SEQ ID Phage display P > > D
A G laccase laccase P350 S349 D80 A79 G78 lac4.2 Ra NO: 178)
PQSDAGVVM (SEQ ID Phage display P > > D A G laccase laccase
P350 S349 D80 A79 G78 lac4.2 Ra NO: 179) GPSRDAGLL (SEQ ID Phage
display P > > D A G laccase laccase P350 S349 D80 A79 G78
lac4.2 Ra NO: 180) PASDAGRGP (SEQ ID Phage display P > > D A
G laccase laccase P350 S349 D80 A79 G78 lac4.2 Ra NO: 181)
APKSDNGIT (SEQ ID Phage display P > > D A G laccase laccase
P350 S349 D80 A79 G78 lac4.2 Ra NO: 182) WPKSDAGDS (SEQ ID Phage
display P > > D A G laccase laccase P300 R234 S211 D213 A296
lac4.3 Ra NO: 183) PQSDAGVVM (SEQ ID Phage display P > > D A
G laccase laccase P300 R234 S211 D213 A296 lac4.3 Ra NO: 184)
GPSRDAGLL (SEQ ID Phage display P > > D A G laccase laccase
P300 R234 S211 D213 A296 lac4.3 Ra NO: 185) PASDAGRGP (SEQ ID Phage
display P > > D A G laccase laccase P300 R234 S211 D213 A296
lac4.3 Ra NO: 186) APKSDNGIT (SEQ ID Phage display P > > D A
G laccase laccase P300 R234 S211 D213 A296 lac4.3 Ra NO: 187)
DPVRDTGAG (SEQ ID Phage display > P > R D T G laccase laccase
P378 R379 D469 T473 G446 lac5.2 Ra NO: 188) PRSDTGFGS (SEQ ID Phage
display > P > R D T G laccase laccase P378 R379 D469 T473
G446 lac5.2 Ra NO: 189) DPARDTGDV (SEQ ID Phage display > P >
R D T G laccase laccase P378 R379 D469 T473 G446 lac5.2 Ra NO: 190)
DPVRDTGAG (SEQ ID Phage display > P > R D T G laccase laccase
P60 R59 D51/D53 lac5.3 Ra NO: 191) T10/T12 G30 PRSDTGFGS (SEQ ID
Phage display > P > R D T G laccase laccase P60 R59 D51/D53
lac5.3 Ra NO: 192) T10/T12 G30 DPARDTGDV (SEQ ID Phage display >
P > R D T G laccase laccase P60 R59 D51/D53 lac5.3 Ra NO: 193)
T10/T12 G30 DPVRDTGAG (SEQ ID Phage display > P > R D T G
laccase laccase P157/P155 R23 lac5.4 Ra NO: 194) D118 T114 G113
PRSDTGFGS (SEQ ID Phage display > P > R D T G laccase laccase
P157/P155 R23 lac5.4 Ra NO: 195) D118 T114 G113 DPARDTGDV (SEQ ID
Phage display > P > R D T G laccase laccase P157/P155 R23
lac5.4 Ra NO: 196) D118 T114 G113
Example 2
Localisation of Epitope Sequences and Epitope Areas on the
3D-Structure of Acceptor Proteins
[0495] Epitope sequences were assessed manually on the screen on
the 3D-structure of the protein of interest, using appropriate
software (e.g. SwissProt Pdb Viewer, WebLite Viewer).
[0496] In a first step, the identified epitope patterns were fitted
with the 3D-structure of the enzymes. A sequence of at least 3
amino acids, defining a specific epitope pattern, was localised on
the 3D-structure of the acceptor protein. Conservative mutations
(e.g. aspartate for glutamate, lysine for arginine, serine for
threonine) were considered as one for those patterns for which
phage display had evidenced such exchanges to occur. Among the
possible sequences provided by the protein structure, only those
were retained where the sequence matched a primary sequence, or
where it matched a structural sequence of amino acids, where each
amino acid was situated within a distance of 5 .ANG. from the next
one. Occasionally, the mobility of the amino acid side chains, as
provided by the software programme, had to be taken in to
consideration for this criterium to be fulfilled.
[0497] Secondly, the remaining anchor amino acids as well as the
variable amino acids, i.e. amino acids that were not defining a
pattern but were present in the individual sequences identified by
phage library screening, were assessed in the area around the
various amino acid sequences localised in step 1. Only amino acids
situated within a distance of 5 .ANG. from the next one were
included.
[0498] Finally, an accessibility criterium was introduced. The
criterium was that at least half of the anchor amino acids had a
surface that was >30% accessible. Typically, 0-2 epitopes were
retained for each epitope pattern. In some cases, two different
amino acids could with equal probability be part of the epitope
(e.g. two leucines located close to each other in the protein
3D-structure). For example, in Savinase two epitopes actually fit
to the antibody binding peptide LDQIFFTRW (SEQ ID NO:62): L75 D41
Q2 179 and L42 D41 Q2 I79. A shorthand notation for such a
situation is: L42/L75 D41 Q2 I79.
[0499] Thus, a number of epitope sequences were identified and
localised on the surface of various proteins. As suggested by
sequence alignment of the antibody binding peptides, structural
analysis confirmed most of the epitopes to be enzyme specific, with
only few exceptions. Overall, most of the identified epitopes were
at least partially structural. However, some proteins (e.g.
amylase) expressed predominantly primary sequence epitopes.
Typically, the epitopes were localised in very discrete areas of
the enzymes, and different epitope sequences often shared some
amino acids (hot-spots).
[0500] The identified epitope sequences are shown in Tables
2-7.
Birch Allergen:
[0501] Bet v1 (WO 99/47680) was used as the parent protein for
identification of epitope sequences that may cross react with
enzyme epitopes. The structural coordinates from 1BV1.pdb (Gajhede
et al., NAT.STRUCT.BIOL., Vol. 3, p. 1040, 1996) were used as well
the corresponding sequence (Swissprot accession number P15494). The
epitope pattern P>PAP>S (which had been identified from
antibody binding peptides specific for anti-Lipolase antibodies)
was found to match three (overlapping) epitope sequences on the
surface of Bet v1:
Bet v1 1.1: P31 A34 P35 A37 P59 S39/S40;
Bet v1 1.2: P63 L62 P59 A37 P35 S39/S40; and
Bet v1 1.3: P59 S39/S40 P31 A34 P35 S39/S40.
Example 3
Epitope Areas
[0502] It is common knowledge that amino acids that surround
binding sequences can affect binding of a ligand without
participating actively in the binding process. Based on this
knowledge, areas covered by amino acids with potential steric
effects on the epitope-antibody interaction, were defined around
the identified epitopes. Practically, all amino acids situated
within 5 .ANG. from the amino acids defining the epitope were
included. The accessibility criterium was not included for defining
epitope areas, as hidden amino acids can have an effect on the
surrounding structures.
[0503] For Savinase, the following amino acid residues belong to
the epitope area that correspond to each epitope sequence indicated
in Table 2:
TABLE-US-00013 sav1.1 A1 Q2 S3 P5 H39 P40 D41 L42 N43 G63 T66 H67
A69 G70 T71 A73 A74 L75 N77 S78 I79 G80 V81 L82 G83 N204 V205 Q206
S207 T208 Y209 P210 S212 T213 Y214 A215 S216 L217 sav1.2 S153 G154
N155 S156 G157 A158 G160 S161 I162 S163 A169 R170 A174 M175 A176
V177 G178 R186 F189 S190 Q191 Y192 G193 A194 G195 L196 D197 I198
V199 T220 R247 K251 A254 T255 S256 T260 N261 L262 Y263 G264 S265
G266 L267 sav2.1 W6 G7 I8 R10 V11 Q12 A13 P14 A15 A16 R19 L21 V84
T180 D181 Q182 N183 N184 I198 V199 A200 P201 H226 V227 A230 L233
V234 K237 N238 H249 L250 T253 A254 T255 S256 L257 S265 G266 L267
V268 N269 A270 E271 A272 A273 T274 R275 sav2.2 S153 G154 N155 S156
G157 A158 S161 I162 S163 G178 A179 T180 D181 N184 N185 R186 A187
S188 F189 S190 Q191 Y192 G193 L196 T220 L262 Y263 sav2.3 A142 T143
G146 V147 L148 Y171 A172 N173 A174 M175 D197 A231 V234 K235 N238
P239 S240 W241 S242 N243 V244 Q245 I246 R247 N248 H249 L250 K251
sav3.1 S153 G154 N155 S156 G157 A158 V177 G178 A179 T180 D181 N184
N185 R186 A187 S188 F189 S190 Q191 Y192 V199 A200 P201 G202 V203
N218 G219 T220 A223 L262 Y263 sav3.2 L111 E112 G115 N116 M119 A138
V139 N140 S141 A142 S144 R145 G146 V147 V149 N173 N243 sav4.0 Q2
H17 T22 G23 S24 G25 V26 K27 V28 V30 I35 S37 T38 H39 P40 D41 L42 N43
I44 R45 G46 T66 A69 G70 T71 I72 A73 A74 L75 N76 N77 I79 G80 V81 L82
G83 V84 A85 P86 S87 A88 E89 L90 Y91 A92 T208 Y209 P210 S212 T213
Y214 sav5.1 A1 Q2 S3 V4 I35 S37 H39 P40 D41 L42 N43 I44 T66 A69 G70
A73 A74 L75 N76 N77 S78 I79 G80 V81 L82 G83 P86 L90 T208 Y214
sav5.2 V30 T33 G34 I35 S37 T38 L42 N43 I44 R45 G46 E54 S57 T58 Q59
D60 G61 N62 G63 H64 G65 T66 H67 A69 L90 Y91 A92 K94 P210 sav5.3 V4
P5 W6 G7 I8 S9 R10 V11 Q12 A13 P14 A15 A16 R19 N269 A270 E271 A272
A273 T274 R275 sav5.4 A1 Q2 P40 D41 F50 L75 N77 S78 I79 G80 V81
V104 S105 S106 I107 A108 Q109 G110 L111 E112 W113 A114 G115 N116
Q137 A138 S141 A142 Y214 sav6.1 V139 N140 T143 L148 V149 A151 P168
A169 Y171 A172 N173 A174 M175 A176 D197 I198 N243 V244 Q245 I246
R247 N248 H249 L250 K251 N252 T253 A254 S265 sav6.2 Q2 G25 V26 K27
V28 A29 I35 S37 T38 H39 P40 D41 L42 N43 I44 R45 G46 G47 Q59 T66 A69
G70 A73 A74 L75 N77 I79 G80 V81 L82 A88 E89 L90 Y91 N117 G118 M119
H120 V121 S207 T208 Y209 P210 G211 S212 T213 Y214 A215 sav7.1 K27
L31 I107 A108 Q109 G110 L111 E112 W113 A114 G115 N116 N117 G118
M119 A122 L124 L135 Q137 A138 V139 S141 A142 R145 V149 sav7.2 V104
I107 A108 L111 S132 A133 T134 L135 E136 Q137 A138 V139 N140 S141
A142 T143 S144 R145 G146 V147 V149 Y167 P168 Y171 A172 N173 A174
M175 N243 R247 sav9.1 L111 E112 A114 G115 N116 M119 H120 V121 A122
E136 Q137 A138 V139 N140 S141 A142 T143 S144 R145 G146 V147 L148
V149 V150 N173 M175 N243 I246 R247 L250 sav9.2 L126 G127 S128 P129
A152 S153 G154 S161 I162 S163 Y167 P168 A169 R170 Y171 A172 A176
V177 G178 Q191 Y192 G193 A194 G195 L196 D197 I198 V199 T260 N261
L262 Y263 G264 sav10.1 Q12 A13 P14 A15 A16 H17 N18 R19 G20 L21 T22
N76 L82 G83 V84 A85 P86 L233 V234 K237 N238 H249 L250 T253 N269
A270 E271 A272 A273 T274 R275 sav10.2 V11 Q12 A13 P14 A15 A16 H17
N18 R19 G20 L21 T22 G23 L233 V234 Q236 K237 N238 H249 L250 T253
A254 T255 L267 V268 N269 A270 E271 A272 A273 T274 R275 sav10.3 L31
D32 H64 V68 V95 L96 I107 L111 A114 G115 N116 M119 V121 A122 N123
L124 S125 L126 G127 S128 P129 V139 S141 A142 T143 S144 R145 G146
V147 L148 V149 V150 A151 A152 S153 S163 Y167 P168 A169 N173 A174
M175 A176 V177 T220 S221 M222 T224 P225 V227 A228 A231 N243 I246
R247 L250 sav10.4 P131 S132 A133 L135 E136 V139 A151 A152 S153 G160
S161 I162 S163 Y167 P168 A169 R170 Y171 A172 N173 A174 A176 Q191
Y192 G193 A194 G195 L196 R247 S259 T260 N261 L262 Y263 G264 sav11.0
W6 G154 N155 S156 G157 A179 T180 D181 Q182 N183 N184 N185 R186 A187
S188 F189 S190 Q191 Y192 P201 G202 V203 N204 V205 L217 N218 G219
T220 L262 Y263 sav12.0 L31 I107 A108 Q109 G110 L111 E112 W113 A114
G115 N116 N117 G118 A122 L124 S132 A133 T134 L135 Q137 A138 V139
N140 S141 T143 R145 V149 A151 S163 Y167 P168 A169 R170 Y171 N173
A174 sav13.0 Q2 S3 P5 T38 H39 P40 D41 L42 N43 H67 G70 A73 A74 L75
N77 I79 G80 V81 L82 G83 V205 Q206 S207 T208 Y209 S212 T213 Y214
A215 S216 L217 sav14.0 A16 H17 R19 G20 L21 T22 G23 S24 G25 V26 K27
V28 A29 V30 I35 I44 R45 G46 G47 V84 A85 P86 S87 A88 E89 L90 Y91 A92
V93 W113 N117 G118 M119 H120 V121 A232 L233 K235 Q236 K237 T274
sav15.0 W6 R10 G154 N155 S156 G157 V177 G178 A179 T180 D181 Q182
N183 N184 N185 R186 A187 S188 F189 S190 Q191 V199 A200 P201 G202
V203 N218 G219 T220 A223 L257 Y263 L267 sav16.0 A13 A16 H17 G20 L21
T22 G23 S24 G25 V26 V28 I72 A73 V84 A85 P86 S87 A88 E89 L90 H120
G229 A230 A231 A232 L233 V234 K235 Q236 K237 N238 P239 S240 W241
I246 H249 L250 A270 A273 T274 sav17.0 T22 G23 S24 G25 V26 K27 V28
A29 V30 L31 D32 I35 I44 R45 G46 G47 A48 F50 S87 A88 E89 L90 Y91 A92
V93 K94 V95 G110 W113 N117 G118 M119 H120 V121 A232 K235 Q236
sav18.1 W6 G7 I8 S9 R10 V11 Q12 A179 T180 D181 Q182 N183 N184 N185
R186 A187 I198 V199 A200 P201 V203 H226 V227 A230 H249 L250 K251
N252 T253 A254 T255 S256 L257 S265 G266 L267 V268 N269 A270 sav18.2
A13 A16 H17 L21 T22 G23 V26 V28 V84 A85 A88 V121 L148 Y171 A172
N173 V174 M175 A176 G195 L196 D197 I198 V199 V227 A228 G229 A230
A231 A232 L233 V234 K235 Q236 K237 N238 W241 N243 V244 Q245 I246
R247 N248 H249 L250 K251 N252 T253 A254 Y263 G264 S265 G266 V268
A270 A273 T274 sav19.1 A16 H17 R19 G20 L21 T22 G23 S24 G25 V26 K27
V28 S87 A88 E89 H120 V121 A232 L233 V234 K235 Q236 K237 N238 P239
T274 sav19.2 A1 Q2 S3 V4 P5 D41 H64 H67 G70 T71 A74 L75 N77 S78 I79
G80 V81 L82 G83 G202 V203 N204 V205 Q206 S207 T208 Y209 Y214 A215
S216 L217 N218 G219 M222
[0504] For PD498, the following amino acid residues belong to the
epitope area that correspond to each epitope sequence indicated in
Table 3:
TABLE-US-00014 pd1.1 D105 A108 S109 G110 I111 R112 Y113 A114 A115
D116 Q117 N131 S132 T133 T134 L135 K136 S137 A138 V139 D140 Y141
A142 W143 N144 K145 G146 A147 pd1.2 C128 E129 A153 G154 N155 D156
N157 V158 S160 R161 T162 F163 Q167 S170 G178 A179 I180 D181 D184
R185 K186 A187 S188 F189 S190 N191 Y192 G193 T194 W195 V196 T220
T262 N263 pd1.3 F50 L104 D105 S106 I107 A108 S109 G110 I111 R112
Y113 A114 A115 D116 Q117 T133 T134 L135 K136 S137 A138 V139 D140
Y141 A142 W143 N144 K145 G146 A147 pd1.4 T28 *28aV A29 V30 D32 S33
G34 V35 Y37 *44aaV I45 K46 G47 Y48 D49 F50 I51 R53 D54 N55 N56 P57
M58 D60 L61 K89 I90 L91 A92 V93 R94 V95 L96 D97 A98 Y113 A114 Q117
A119 pd1.5 D32 S33 G34 K46 G47 Y48 D49 F50 I51 D52 R53 D54 N55 P57
M58 L61 L91 A92 V93 R94 V95 L96 D97 A98 L104 D105 S106 I107 A108
S109 G110 I111 R112 Y113 A114 A115 D116 Q117 G118 A119 T133 T134
L135 K136 S137 A138 V139 D140 Y141 A142 pd2.1 V19 T21 I111 R112
Y113 A114 A1 D116 Q117 G118 A119 L122 D140 Y141 A142 W143 N144 K145
G146 A147 V148 L233 L234 A235 S236 Q237 G238 K239 N240 N243 V244
Q245 I246 R247 Q248 A249 A273 V274 R275 Y276 pd2.2 S24 S25 T26 Q27
T28 *28aV L42 A43 R44 *44aK *44aaV I45 D75 N77 D87 T88 K89 I90 L91
G118 A119 K120 V121 L122 G146 A147 V148 A232 A235 S236 pd2.3 R22
G23 S24 S25 T26 Q27 T28 *28aV D87 T88 K89 I111 A115 G118 A119 K120
V121 L122 S137 A138 V139 D140 Y141 A142 W143 N144 K145 G146 A147
V148 V149 V150 I175 A231 A232 A235 S236 N243 I246 R247 pd2.4 W-6
S12 T13 P14 A15 A16 V19 T21 R22 G23 S24 Q27 L230 A231 L233 L234
A235 S236 Q237 G238 K239 N240 N243 Q245 I246 S270 N271 K272 A273
V274 R275 Y276 pd3.1 L31 K46 G47 Y48 F50 L91 V93 S103 L104 D105
S106 I107 A108 S109 G110 I111 R112 Y113 A114 A115 D116 Q117 G118
L122 L124 C130 S132 T133 T134 L135 K136 S137 A138 V139 D140 Y141
A142 Q167 P168 Y171 P172 pd3.2 V19 T21 R22 G23 S24 Q27 K120 V121
V148 L230 A231 A232 L233 L234 A235 S236 Q237 G238 K239 N240 N243
Q245 I246 R247 Q248 A249 I250 Q252 T253 K272 A273 V274 R275 Y276
pd4.1 W-6 S12 T13 P14 A15 A16 W17 D18 V19 T21 R22 G23 S24 M84 A85
P86 D87 T88 A142 W143 G146 A147 V148 G229 L230 A231 A232 L233 L234
A235 S236 Q237 G238 K239 N240 N243 V244 Q245 I246 R247 Q248 A249
I250 S270 N271 A273 V274 R275 Y276 pd4.2 W-6 T13 A16 W17 V19 T21
R22 G23 S24 *44aK A73 A74 *75aT G83 M84 A85 P86 D87 T88 A142 G146
G146 A147 V148 G229 L230 A231 A232 L233 L234 A235 S236 Q237 G232
K239 N240 N243 V244 Q245 I246 R247 Q248 A249 I250 S270 A273 V274
R275 Y276 pd4.3 T26 Q27 T28 *28aV A29 V30 L31 Y37 *44aaV I45 K46
G47 Y48 D49 D52 R53 D54 N55 N56 P57 M58 V72 T88 K89 I90 L91 A92 V93
Y113 A114 A115 Q117 G118 A119 K120 V121 L122 N123 A147 A228 A232
pd4.4 K46 G47 F50 L91 V93 S103 L104 D105 S106 I107 A108 S109 G110
I111 R112 Y113 A114 A115 D116 Q117 G118 C130 S132 T133 T134 L135
K136 S137 A138 V139 D140 Y141 Q167 P168 A169 S170 Y171 P172 N173
A174 pd4.5 T28 *28aV A29 V30 L31 V35 D36 Y37 N38 H39 L42 A43 *44aaV
I45 K46 G47 Y48 F50 N55 N56 P57 M58 K89 I90 L91 A92 V93 A108 S109
G110 I111 R112 Y113 A114 A115 D116 Q117 G118 A119 L122 pd5.0 F50
S103 L104 D105 S106 I107 A108 S109 G110 I111 R112 Y113 A114 A115
D116 Q117 T133 T134 L135 K136 S137 A138 V139 D140 Y141 A142 pd6.1
Y4 Y6 G7 G63 H64 H67 V68 T71 N155 A179 F189 P201 G202 V203 N204
I205 A206 S207 V209 G213 Y214 S215 Y216 M217 S218 G219 T220 S221
M222 A223 S224 P225 H226 pd6.2 W-6 T13 A16 W17 V19 T21 R22 G23 S24
S25 Q27 M84 A85 P86 D87 T88 G229 L230 A231 A232 L233 L234 A235 S236
Q237 G238 S270 V274 pd7.0 R22 G23 S24 S25 Q27 T28 *28aV A29 V30 V35
D36 Y37 N38 H39 P40 D41 L42 A43 R44 *44aK *44aaV T66 A69 G70 V72
A73 A74 D75 N77 A85 P86 D87 T88 K89 I90 L91 A119 V121 L122 N123
T208 A228 A231 pd8.0 W-6 T13 A16 W17 T21 R22 G23 Q27 *44aK A73 A74
*75aT G83 M84 A85 P86 D87 T88 K120 V121 I175 A176 V177 G178 V196
D197 V198 T199 A200 V227 G229 L230 A231 A232 L233 L234 A235 S236
Q237 G238 K239 N240 N243 Q245 I246 Q248 A249 I250 Q252 T253 A254
F264 Y265 G266 I268 pd9.0 W-6 Y6 G7 P8 Q9 N10 T11 S12 T13 P14 A15
A16 W17 D18 V19 T21 M84 V139 W143 V148 V149 A151 P168 A169 Y171
P172 N173 A174 I175 A176 D181 S182 N183 D184 D197 P201 L230 L233
L234 K239 N240 N243 V244 Q245 I246 R247 Q248 A249 I250 E251 Q252
T253 A254 K267 I268 N269 S270 N271 K272 A273 V274 R275 Y276 pd10.0
L124 L126 G127 C128 E129 C130 N131 L135 V139 A151 A152 A153 G154
N155 D156 N157 V158 S160 R161 T162 F163 Q167 P168 A169 S170 Y171
A174 I175 A176 N191 Y192 G193 T194 W195 V196 T262 N263 F264 *264aK
pd11.0 W-6 S-5 Y2 Y4 Q5 Y6 G7 P8 Q9 N10 T11 S12 T13 P14 W17 D18 V19
T21 A82 M84 I180 D181 S182 N183 D184 P201 G202 V203 N204 I205 H226
L233 S270 N271 V274 R275 pd12.0 G127 C128 E129 V139 V148 V149 V150
A151 A152 A153 G154 N155 D156 V158 R161 T162 F163 Q167 P168 A169
S170 Y171 P172 N173 A174 I175 A176 V177 G178 N191 Y192 G193 T194
W195 V196 D197 V198 T199 A200 V227 R247 I250 E251 A254 N263 F264
*264aK Y265 G266 I268 pd13.1 W-6 S-5 P-4 D-2 P-1 Y1 Y2 S3 *3aA Y4
Q5 P8 Q9 S12 T13 P14 A15 A16 W17 D18 V19 T21 R22 G80 V81 A82 N271
V274 R275 pd13.2 W-6 S-5 P-4 N-3 D-2 P-1 Y1 Y2 S3 *3aA Y4 Q5 P8 Q9
P14 W17 D41 G70 A74 D75 *75aT N76 N77 G78 I79 G80 V81 A82 G83 A206
S207 T208 Y214 pd14.0 T28 V35 D36 Y37 N38 H39 P40 D41 L42 A43 R44
*44aK *44aaV I45 K46 G47 Y48 D49 F50 R53 D54 N55 N56 P57 M58 T66
A69 G70 A73 A74 D75 K89 I90 L91 A92 V93 R94 Y113 T208 pd15.0 V30
L31 D32 S33 G34 V35 D36 Y37 N38 H39 L42 A43 *44aaV K46 Y48 D49 F50
I51 N56 P57 M58 D60 L61 N62 G63 H64 G65 T66 A69 I90 A92 V93 R94 V95
L96 D97 A98 G100 S101 G102 S103 S106 I107 G110 S125 L126 V209 P210
N211 N212 pd16.0 W-6 S-5 P-4 N-3 Y2 G7 P8 Q9 N10 T11 S12 T13 P14
A15 A16 W17 D18 V19 T21 R22 *75aT N76 A82 G83 M84 A85 P86 L233 N269
S270 N271 pd17.1 T11 S12 A15 A16 D18 V19 T21 R22 G23 S24 Q27 L230
A232 L233 L234 A235 S236 Q237 G238 K239 N240 N243 Q245 I246 Q248
A249 Q252 T253 N269 S270 N271 K272 A273 V274 R275 Y276 pd17.2 A108
I111 R112 A115 D116 K120 L124 T133 T134 L135 K136 S137 A138 V139
D140 Y141 A142 W143 N144 K145 G146 A147 V148 V149 P168 Y171 N173
A174 N243 pd18.1 W-6 T13 A16 W17 V19 T21 R22 G23 S24 S25 *44aK M84
A85 P86 D87 T88 K89 G229 L230 A231 A232 L233 L234 A235 S236 Q237
K239 A249 I250 T253 N269 S270 N271 K272 A273 V274 R275 Y276 pd18.2
D-2 V30 V35 D36 Y37 N38 H39 P40 D41 L42 A43 R44 *44aK *44aaV I45
K46 G47 Y48 P57 T66 A69 G70 A73 A74 D75 *75aT N76 N77 I79 V81 A82
A85 P86 D87 T88 K89 I90 L91 A92 V93 R94 T208
[0505] For Lipolase, the following amino acid residues belong to
the epitope area that correspond to each epitope sequence indicated
in Table 4:
TABLE-US-00015 lip2.1 Y53 F55 V63 L78 F80 W117 V120 A121 D122 T123
L124 R125 Q126 K127 V128 E129 D130 A131 V132 R133 V140 L159 R160
G161 N162 G163 Y164 D165 I166 G190 lip2.2 V2 L6 F10 A173 P174 R175
A182 L193 Y194 R195 I196 T197 P204 R205 Y213 S214 H215 S216 S217
P218 E219 Y220 W221 I222 I235 V236 K237 I238 E239 I241 D242 A243
G246 N247 N248 lip2.3 V2 L6 F10 A182 L185 T186 L193 Y194 R195 I196
T197 H215 S216 S217 P218 E219 Y220 W221 I222 I235 V236 K237 I238
E239 G240 I241 A243 G246 N247 N248 lip2.4 V2 L6 F10 L193 Y194 R195
I196 T197 S216 S217 P218 E219 Y220 W221 I222 I235 V236 K237 I238
E239 G240 A243 G246 N247 N248 lip3.0 L93 K94 F95 H110 A173 P174
R175 V176 G177 N178 R179 A182 L185 T186 L193 R195 N200 D201 I202
P204 R205 L206 P207 P208 R209 E210 F211 G212 Y213 S214 H215 S216
S217 P218 E219 I238 E239 G240 I241 D242 A243 T244 G245 N248 ?R259?
P250 N251 I252 P253 D254 I255 lip4.0 R175 V176 G177 N178 R179 A180
F181 A182 E183 F184 L185 T186 R205 P207 P208 R209 E210 F211 G212
Y213 S214 H215 S216 S217 I241 D242 N248 lip5.1 A20 Y21 N25 N26 T50
F51 L52 Y53 S54 F55 E56 V63 T64 G65 F66 L67 A68 L69 I76 V77 L78 S79
F80 R81 G82 S83 R84 S85 I86 E87 N88 W89 K127 V128 A131 H145 S146
L147 G148 L151 G266 lip5.2 K94 F95 L96 L97 K98 E99 R108 G109 H110
D111 G112 R175 V176 G177 N178 R179 A180 F181 A182 E183 F184 R205
P207 P208 R209 E210 F211 G212 Y213 S214 H215 S216 I241 D242 N248
lip6.0 Q9 F10 N11 F13 A14 S17 V63 F80 R81 W89 L93 F113 S116 W117
F142 T143 G144 H145 S146 L147 G148 G149 A150 L151 A152 T153 V154
A155 G156 A157 V168 F169 S170 Y171 G172 A173 P174 R175 V176 F181
L185 L193 Y194 R195 I196 T197 D201 V203 P204 L206 P207 H215 H258
Y261 F262 I265 lip7.0 F13 A14 Q15 Y16 S17 A180 A19 A20 Y21 C22 G23
N25 N26 I34 C36 A40 C41 F51 L52 Y53 S54 F55 E56 V63 T64 G65 F66 L67
S79 F80 R81 V120 A121 D122 T123 L124 R125 Q126 K127 V128 L264 I265
lip8.1 L12 F13 A14 Q15 Y16 S17 A18 A19 A20 I34 V44 A49 T50 F51 L52
F66 L67 A68 L69 D70 N71 T72 N73 K74 L75 I76 V77 S79 H135 P136 D137
Y138 R139 V140 V141 T143 lip8.2 L12 F13 A14 Q15 Y16 S17 A18 A19 A20
I34 V44 A49 T50 F51 L52 Y53 S54 F55 G65 F66 L67 A68 L69 D70 N73 L75
I76 V77 L78 S79 T123 L124 R125 Q126 K127 V128 E129 D130 A131 T143
lip9.0 L6 F10 N25 N26 D27 A28 A30 G31 T50 F51 L52 Y53 S54 F55 E56
G65 F66 L67 A68 L69 I76 T123 L124 R125 Q126 K127 V128 E129 D130
A131 V132 R1333 E134 H135 P136 R139 V140 V141 F142 G156 L159 R160
G161 N162 G163 Y164 D165 I166 D167 V168 F169 S170 G190 G191 T192
L193 Y194 R195 I196 Y220 lip10.0 N11 L12 Q15 Y16 I34 T35 C36 C41
P42 E43 V44 E45 K46 A47 D48 A49 D70 N71 T72 N73 K74 lip11.0 F95 L96
L97 K98 E99 I100 N101 D102 C107 R108 G109 H110 D111 F113 T114 S115
A150 T153 V154 A173 P174 R175 V176 G177 N178 R179 F181 V203 P204
R205 L206 P207 P208 R209 F211 G212 Y213 S214 H215 G240 I241 D242
A243 T244 N248 lip12.0 L96 L97 K98 E99 I100 N101 D102 C104 S105
G106 C107 R108 G109 H110 T114 S115 V176 G177 N178 A180 F181 F184
lip13.0 N11 L12 F13 A14 Q15 Y16 S17 A182 A19 A20 Y21 N26 I34 C36
A40 C41 P42 E43 V44 A49 F55 E56 V63 T64 G65 F66 L67 A68 D70 N73 L75
I76 V77 L78 S79 F80 R81 G82 S83 R84 W89 W117 L124 V128 V141 F142
T143 G144 H145 S146 L147 G148 G149 A150 L151 A152 A155 lip14.0 Q9
F10 N11 F13 A14 S17 Y21 R81 G82 S83 R84 S85 I86 E87 N88 W89 I90 G91
N92 L93 F113 T143 G144 H145 S146 L147 G149 A150 T153 V168 F169 S170
Y171 A173 P174 R175 V176 L193 Y194 R195 I196 T197 D201 V203 P204
L206 P207 H215 H258 Y261 F262 I265 G266 lip15.0 N11 L12 F13 A14 Q15
Y16 S17 A18 A19 A20 Y21 C22 G23 K24 N25 N26 D27 A28 I34 T35 C36 A40
C41 P42 E43 V44 E45 K46 A47 A49 F51 L52 Y53 S54 F55 E56 T64 G65 F66
L67 S79 F80 R81 T123 L124 K127 L264 I265 lip16.0 A14 E87 I90 H145
G172 I196 T197 H198 T199 N200 D201 I202 P204 R205 W221 I222 K223
S224 G225 T226 G246 N247 N254 I252 P253 D254 I255 P256 A257 H258
L259 W260 Y261 F262 G263 I265 lip17.0 E1 V2 F7 F10 G177 N178 R179
A180 F181 A182 E183 F184 L185 T186 L193 R195 H198 T199 G212 S214
H215 S216 S217 P218 E219 Y220 W221 I222 K223 S224 G225 T226 V228
P229 V230 T231 R232 N233 D234 I235 V236 K237 I238 E239 G240 I241
D242 A243 T244 G245 G246 I262 lip18.0 Q9 F13 Y16 T32 N33 I34 C41
P42 E43 V44 E45 K46 A47 D48 A49 T50 F51 L52 L67 A68 L69 D70 N71 T72
N73 L75 I76 V128 V132 H135 P136 D137 Y138 R139 V140 V141 F142 Y164
D165 I166 D167 F169 Y194
[0506] For Amylase, the following amino acid residues belong to the
epitope area that correspond to each epitope sequence indicated in
Table 5:
TABLE-US-00016 je1.1 N2 G3 T4 R33 P346 Y349 I352 L353 T354 R355
P360 V362 D366 Y367 M378 K379 A380 K381 I382 D383 P384 I385 L386
E387 A388 R389 Q390 N391 F392 A393 Y394 I450 T451 je1.2 Y57 D58 Y60
D61 F65 N66 Q67 L104 G105 G106 A107 D108 A109 T110 E111 A135 W136
T137 K138 F139 D140 F141 P142 G143 R144 G145 N146 T147 Y148 S149
F151 K152 W153 R154 F158 je2.1 M6 Y8 E10 W11 H12 D26 L30 R33 V325
D326 N327 H328 D329 S330 Q331 P332 G333 E334 E337 F339 K345 Y349
V362 F363 Y364 G365 D366 Y367 Y368 G369 I370 P371 T372 H373 S374
V375 P376 A377 M378 K379 I382 D383 L386 je2.2 L289 L293 V314 P318
T323 F324 V325 D326 F339 K345 P346 L347 A348 Y349 A350 L351 I352
L353 T354 R355 F356 Q357 G358 Y359 P360 S361 V362 F363 Y364 G365
D366 Y367 Y368 G369 P376 A377 M378 K379 I382 I385 R389 Q397 je2.3
N102 V116 E117 V118 P120 R123 D159 G160 V161 D162 W163 Q168 F169
Q170 N171 R172 I173 Y174 K175 A182 W183 D184 V187 D188 N193 Y194
D195 Y196 L197 M198 Y199 A200 D201 V202 H236 je2.4 T1 N2 T4 M6 Y8
D26 L30 R31 N32 R33 G34 I35 V325 D326 F339 K345 Y349 L353 V362 F363
Y364 G365 D366 Y367 Y368 G369 I370 P376 A377 M378 K379 I382 D383
P384 I385 L386 E387 A388 R389 Q390 N391 F392 Y394 H417 je3.1 M6 Q7
Y8 F9 E10 L13 H19 W20 N21 R22 L23 R24 D25 D26 A27 S28 N29 L30 R31
N32 R33 I385 W39 I40 P41 P42 A43 W44 V52 G53 Y54 Y75 A87 L88 N91
V93 D98 V100 Y364 Y368 je3.2 Y8 F9 W11 H19 W20 W39 I40 P41 P42 A43
W44 D51 V52 G53 Y54 G55 A56 Y75 D98 V99 V100 M101 N102 H103 L104
D195 L197 M198 A200 D201 V202 R230 I231 D232 A233 V234 K235 H236
I237 E262 H328 je3.3 Y8 F9 H19 W20 W39 I40 P41 P42 A43 W44 K45 G46
T47 V52 G53 Y54 G55 A56 Y57 D58 L59 Q67 K68 Y75 D98 V100 L104 G105
G106 A107 D108 A109 T110 E111 A135 W136 T137 K138 F139 D140 F141
P142 je4.1 L23 D25 D26 A27 S28 N29 L30 R31 N32 R33 G34 I35 T36 I38
A84 I85 H86 A87 L88 K89 N90 N91 G92 V93 Q94 V95 Q390 je4.2 A43 W44
K45 L59 Y60 D61 L62 G63 E64 F65 V71 R72 T73 K74 Y75 G76 T77 R78 S79
Q80 L81 E82 S83 Y148 W219 Y220 T223 L224
Example 4
[0507] Having identified `antibody binding peptide` sequences and
by consensus analysis also "epitope patterns" (e.g.
>DF>>K>), one can identify potential epitope sequences
on the 3-dimensional surface of a parent protein (=acceptor
protein) in a semi-automated manner using the following method:
[0508] The anchor amino acid residues are transferred to a three
dimensional structure of the protein of interest, by colouring D
red, F white and K blue. Any surface area having all three residues
within a distance of 18 .ANG., preferably 15 .ANG., more preferably
12 .ANG., is then claimed to be an epitope. The relevant distance
can easily be measured using e.g. molecular graphics programs like
Insight!! from Molecular Simulations Inc.
[0509] The residues in question should be surface exposed, meaning
that the residue should be more than 20% surface exposed,
preferably more than 50% surface exposed, more preferably 70%
surface exposed. The percentage "surface accessible area" of an
amino acid residue of the parent protein is defined as the Connolly
surface (ACC value) measured using the DSSP program to the relevant
protein part of the structure, divided by the residue total surface
area and multiplied by 100. The DSSP program is disclosed in W.
Kabsch and C. Sander, BIOPOLYMERS 22 (1983) pp. 2577-2637. The
residue total surface areas of the 20 natural amino acids are
tabulated in Thomas E. Creighton, PROTEINS; Structure and Molecular
Principles, W.H. Freeman and Company, NY, ISBN: 0-7167-1566-X
(1984).
[0510] Substitutions of one or more residue (s) within 18 .ANG.,
preferably 15 .ANG., more preferably 12 .ANG., around the
geometrical center of the residues involved in the epitope, for a
bigger or smaller residues, may destroy the epitope, and make the
protein less antigenic.
[0511] Residues involved in epitope is 2, preferably 3 and more
preferably 4
Example 5
Production, Selection, and Evaluation of Enzyme Variants with
Reduced Antiqenicity or Immunogenicity
[0512] Epitope sequences and hot-spots amino acids were mutated
using standard techniques know to the person skilled in the field
(e.g. site-directed mutagenesis, error-prone PCR--see for example
Sambrook et al. (1989), Molecular Cloning. A Laboratory Manual,
Cold Spring Harbour, N Y).
[0513] In the examples shown below, variants were made by
site-directed mutagenesis. Amino acid exchanges giving new epitopes
or duplicating existing epitopes, according to the information
collected in the epitope-database (See Example 1), were avoided in
the mutagenesis process.
[0514] Enzyme variants were screened for reduced binding of
antibodies raised against the backbone enzyme. Antibody binding was
assessed by competitive ELISA as described in the Methods
section.
[0515] Variants with reduced antibody binding capacity were further
evaluated in the mouse SC animal model (See methods section).
[0516] The following variants showed reduced IgE and/or reduced IgG
levels in the mouse model:
TABLE-US-00017 Parent % IgG % IgE protein Mutations Target epitope
sequences response response Savinase D181N Sav11.0; Sav15.0 and
Sav18.1. 50 19 Hot spot amino acid. Savinase R170L; Q206E Sav9.4;
Sav10.4; Sav1.1; and 5 34 Sav19.2 Savinase R170L, S57P Sav9.4;
Sav10.4 45 12 Savinase R247E Sav2.3, Sav6.1, Sav18.2 75 30 Hot spot
amino acid. Savinase R247Q Sav2.3, Sav6.1, Sav18.2 17 20 Hot spot
amino acid. Savinase R247H Sav2.3, Sav6.1, Sav18.2 40 27 Hot spot
amino acid. Savinase R247K Sav2.3, Sav6.1, Sav18.2 74 34 Hot spot
amino acid.
Example 6
Production, Selection, and Evaluation of Enzyme Variants with
Reduced Antigenicity or Immunogenicity
[0517] Hot-spots or epitopes were mutated using techniques known to
the expert in the field (e.g. site-directed mutagenesis,
error-prone PCR).
[0518] In the examples showed below, variants were made by
site-directed mutagenesis. Amino acid exchanges giving new epitopes
or duplicating existing epitopes according to the information
collected in the epitope-database, were avoided in the mutagenesis
process.
[0519] Enzyme variants were screened for reduced binding of
antibodies raised against the backbone enzyme. This antibody
binding was assessed by established assays (e.g. competitive ELISA,
agglutination assay).
[0520] Variants with reduced antibody binding capacity were further
evaluated in animal studies.
[0521] Mice were immunised subcutanuous weekly, for a period of 20
weeks, with 50 microliters 0.9% (wt/vol) NaCl (control group), or
50 microliters 0.9% (wt/vol) NaCl containing 10 micrograms of
protein. Blood samples (100 microliters) were collected from the
eye one week after every second immunization. Serum was obtained by
blood clothing, and centrifugation.
[0522] Specific IgG1 and IgE levels were determined using the ELISA
specific for mouse or rat IgG1 or IgE. Differences between data
sets were analysed by using appropriate statistical methods.
A. Site-Directed Mutagenesis of Amino Acids Defining Epitopes, with
an Effect on IgG1 and/or IgE Responses in Mice.
Epitope: A172/A169 R170A194 G193 N261
Pattern: AR>R>A>N
Antibody: IgG1+IgE
Backbone: Savinase
[0523] The variant carried the mutation R170F.
[0524] In a competitive IgE ELISA, this variant was less effective
in competing for anti-savinase antibodies, giving a 15% lower
endpoint inhibition as compared to the savinase backbone.
[0525] Mouse studies revealed an 80% reduction of the specific IgE
levels, as compared to savinase backbone (p<0.01). The IgG1
levels were not significantly affected.
Epitope: S216 E219 Y220
Pattern: E Y>M
Antibody: IgG1
Backbone: Lipoprime
[0526] The variant carried the mutation S216W.
[0527] In a competitive IgG ELISA, the variant was less effective
in competing for Lipolase antibodies, giving a 38% decrease in
endpoint inhibition as compared to the enzyme backbone.
[0528] Mouse studies revealed a 69% decrease in specific IgG1
levels, compared to the lipolase backbone (p<0.05). The IgE
levels were not significantly affected.
B. Site-Directed Mutagenesis of Epitopes, with Examples of Epitope
Duplication, and New Epitope Formation, Respectively, Predicted by
the Epitope-Database.
Epitope: T143 N173 N140 E136 L135
Pattern: S/T NN>EL
Antibody: IgG1
Backbone: Savinase
[0529] The variant carried the mutation E136R.
[0530] In a competitive IgG ELISA, the variants were less effective
in competing for savinase antibodies, giving a 38% decrease in
endpoint inhibition as compared to the savinase backbone.
[0531] Mouse studies revealed a dramatic increase in specific IgG1
levels, compared to savinase backbone (p<0.01). The IgE levels
were not significantly affected.
[0532] Mutation E136R establishes an IgG1 epitope of the R Y P R/K
pattern, previously identified on PD498. Apparently, this new
epitope was more antigenic in mice than the existing epitope. The
introduction of a savinase unrelated epitope on the savinase
backbone could explain the observed discrepancy between competitive
ELISA and animal studies.
[0533] In this example, it was found that using information derived
exclusively from screening phage libraries with anti-PD498
antibodies (to identify the R Y P R/K epitope pattern of Table 2)
one could predict the outcome of a genetic engineering experiment
for Savinase in which the E136R mutation created the PD498-epitope
on the Savinase surface, leading to increased immunogenicity of
this Savinase variant. This demonstrates that the epitope patterns
identified may be used to predict the effect on immunogenicity of
substitutions in proteins that are different from the parent
protein(s) used to identify the epitope pattern.
C. Site-Directed Mutagenesis of Amino Acids Defining Epitope Areas,
with a Differential Effect on IgG1 and IgE Antibody Levels in Mice,
and an Inhibiting Effect on IgG Binding, Respectively.
Epitope: A172/A169 R170A194 G193 N261
Pattern: AR>R>A>N
Antibody: IgG1+IgE
Backbone: Savinase
[0534] Epitope area: P131, S132, A133, L135, E136, V139, A151,
A152, S153, G161, S162, I165, S166, Y167, P168, Y171, N173, A174,
A176, Q191, Y192, G195, L196, R247, S259, T260, L262, Y263,
G264.
[0535] The variant was different at position Y167 by the mutation
Y167I.
[0536] In a competitive IgE ELISA, the variant was less effective
in competing for anti-savinase antibodies, giving a 8% lower
endpoint inhibition as compared to the its backbone.
[0537] Mouse studies revealed a 75% reduction of the specific IgE
levels, as compared to the backbone (p<0.01). In contrast, the
IgG1 levels were dramatically increased (p<0.01).
Epitope: T143 N173 N140 E136 L135
Pattern: S/T NN>EL
Antibody: IgG1
Backbone: Savinase
[0538] Epitope area: V10A, 1107, A108, L111, E112, G115, S132,
A133, T134, Q137, A138, V139, S141, A142, S144, R145, G146, V147,
V149, Y167, P168, Y171, A172, A174, M175, N243, R247.
[0539] While variant no. 1 was mutated at the epitope position
(N140D), variant no. 2 was mutated at N140 (N140D), but also at the
epitope area position (A172D).
[0540] In a competitive IgG ELISA, variant no. 1 was less effective
in competing for anti-savinase antibodies, as compared to savinase.
This variant revealed a 21% lower endpoint inhibition as compared
to the its backbone.
[0541] Variant no. 2 resulted in an endpoint inhibition that was
60% lower as compared to savinase, and 40% as compared to variant
no. 1.
Example 7
Conjugation of Savinase Variant E136K with Activated
Bis-PEG-1000
[0542] 4.9 mg of the Savinase variant was incubated in 50 mM Sodium
Borate pH 9.5 with 12 mg of N-succinimidyl carbonate activated
bis-PEG 1000 in a reaction volume of approximately 2 ml. The
reaction was carried out at ambient temperature using magnetic
stirring while keeping the pH within the interval 9.0-9.5 by
addition of 0.5 M NaOH. The reaction time was 2 hours.
[0543] The derivatives was purified and reagent excess removed by
size exclusion chromatography on a Superdex-75 column (Pharmacia)
equilibrated in 50 mM Sodium Borate, 5 mM Succinic Acid, 150 mM
NaCl, 1 mM CaCl.sub.2 pH 6.0.
[0544] The conjugate was stored at -20.degree. C., in the above
described buffer.
[0545] Compared to the parent enzyme variant, the protease activity
of the conjugate was retained (97% using Dimethyl-casein as
substrate at pH 9).
Example 8
[0546] Competitive ELISA was performed according to established
procedures. In short, a 96 well ELISA plate was coated with the
parent protein. After proper blocking and washing, the coated
antigen was incubated with rabbit anti-enzyme polyclonal antiserum
in the presence of various amounts of modified protein (the
competitior).
[0547] The amount of residual rabbit antiserum was detected by pig
anti-rabbit immunoglobulin, horseraddish peroxidase labelled.
TABLE-US-00018 Epitope: T143 N173 N140 E136 L135 Pattern: S/T N N
> E L Antibody: IgG1 Backbone: Savinase Mutation: E136K
Modification: bis-NHS-PEG1000
[0548] The data show that the derivative (60% endpoint inhibition)
has reduced capacity to bind enzyme specific immunoglobulines, as
compared to the parent protein (100% endpoint inhibition).
Example 9
[0549] For this example the epitope sequences were determined in
four environmental allergens (Bet v1; Der f2; Der p2 and PhI p2),
based on their structures (1btv.pdb; 1ahm.pdb; a19v.pdb; and
1whp.pdb, respectively), sequences (SEQ ID NOS: 6, 7, 8 and 9,
respectively) and computer modelling of the epitope patterns that
had been assembled in our database (shown in Table 8). The
allergens arise from common sources of allergy: Birch (Bet v1 from
Betula pendula), House dust mites (Der f2 from Dermatophagoides
farinae and Der p2 from Dermatophagoides pteronyssinus), and
Timothy grass (PhI p2 from Phleum pratense).
[0550] The protein surface is scanned for epitope patterns matching
the given "consensus" sequence of about 6-12 residues. First,
residues on the protein surface that match the first residue of the
consensus sequence are identified. Within a specified distance from
each of these, residues on the protein surface that match the next
residue of the consensus sequence are identified. This procedure is
repeated for the remaining residues of the consensus sequence. The
method is further described under the paragraph "Methods" above and
the computer program can be found in the Appendixes.
[0551] The critical parameters used in this screening included:
[0552] i) a maximal distance between the alpha-carbon atoms of
subsequent amino acids, [0553] ii) a minimal accessibility of the
amino acid of 20 .ANG.2, [0554] iii) the largest maximal distance
between the most distinct amino acids should be less than 25 .ANG.,
[0555] iv) the 5 best epitopes were taken, [0556] v) the minimal
homology with the epitope pattern of interest was 80%
[0557] In this way a number of potential epitopes are identified.
The epitopes are sorted according to total surface accessible area,
and certain entries removed: [0558] 1) Epitopes that contain the
same protein surface residue more than once. These are artefacts
generated by the described algorithm. [0559] 2) Epitopes which are
"too big", i.e. where a distance between any two residues in the
epitope exceeds a given threshold.
[0560] The epitope sequences found by this second generation
mapping procedure were:
Bet v1:
Epi#02
A146, K32, Q36, F30, T142, R145, V12
A34, K32, Q36, F30, T142, R145, V12
Epi#03
L62, K65, - - - , 156, Y66
L24, K20, H76, I23, Y81
L24, K20, H76, 1104, Y81
Epi#04
K134, S136, Q132, K129, A130, A135
K134, S136, Q132, K129, V128, G1
Epi#05
G140, A146, R145, T10, G111, A106, T107, V12
G26, A146, R145, T10, G110, A106, T107, V12
G140, A146, R145, T10, G110, S11, S149, L152
G110, A106, S11, T9, G140, R145, T10, V12
G140, A146, R145, T10, G111, S11, S149, V12
Epi#06
G110, P108, D109, T107, A106, P14
G111, P108, D109, T107, A106, P14
A34, N28, D27, S40, K32, P35
G26, N28, D27, S39, K32, P35
A106, N78, D75, T77, A16, P14
G26, N28, D27, S39, Q36, P35
Epi#07
G46, T52, D69, S99, R70, V71, P50, D72
G49, T52, D69, S99, R70, V71, P50, D72
G48, T52, D69, S99, R70, V71, P50, D72
Epi#08
K123, E127, G1, V2, H121, F3
K65, E60, F64, V67, F58
K65, E60, F58, V67, F64
K129, E127, G1, V2, H121, F3
Epi#09
S149, L152, D156, N159, R17, L24, D75, K103, N78, A106, V12
L152, S149, D156, N159, R17, L24, D75, H76, N78, A106, V12
L152, D156, N159, R17, L24, D75, K80, N78, A106, V12
Epi#10
D109, A106, N78, T77, F79, R17, K20
E141, T10, R145, T142, F30, G26, K32
E8, T10, R145, T142, F30, G26, K32
Epi#11
F30, K32, I38, Q36, V33, E148
F22, F30, I38, Q36, V33, E148
F30, L143, 138, Q36, V33, E148
Epi#12
Y5, E6
Y83, E73
Y120, E127
Y5, E8
Y66, E87
Y81, E73
Epi#13
H76, A16, P14, T107, A106, P108, G110, G111
A16, R17, P14, T107, A106, P108, G110, G111
A157, R17, P14, T107, A106, P108, G111, G110
Epi#15
K65, P90, D93, I91, K97, G92
K32, P31, D27, I56, K65, G61
Epi#17
A153, S149, R145, S11
A106, S11, R145, S149
Epi#18
R145, S149, L152, A153, Y150, L151, H154, S155
R145, S149, L152, A153, S155, L151, A157, N159
Epi#22
D125, D93, P90, K65
D93, P90, P63, E60
Epi#23
K55, N43, E42, S57, L62, P63
K68, N43, E42, S40, F30, P35
K54, N43, E42, S57, F64, P63
K55, N43, E42, S40, F58, P35
Epi#24
E96, K97, E87, P90, F64, E60, K65
E127, K123, E96, P90, F64, P63, K65
E42, K68, E87, P90, F64, E60, K65
E42, K55, E87, P90, F64, E60, K65
D93, G92, E87, P90, F64, E60, K65
D125, K123, E96, P90, F64, P63, K65
Epi#25
R70, K55, I44, E45, E42
R70, K54, I44, E45, N47
R70, K68, I53, E45, N47
Epi#27
D93, E127, D125, K123
Epi#28
A146, Q36, F58, E60, L62, F64, P63, K65
I38, Q36, F58, E60, L62, F64, P63, K65
A34, Q36, F58, E60, L62, F64, P63, K65
L143, Q36, F58, E60, L62, F64, P63, K65
V33, Q36, F58, E60, L62, F64, G61, K65
Epi#29
G61, K65, L62, F58, E60
I56, K65, L62, F64, E60
G89, K65, L62, F64, E60
V67, K65, L62, F64, E60
Epi#30
G1, N4, S99, H121, K97, I91, P90
I113, I13, S149, H154, S155, L152, L151
I13, L152, A153, H154, S155, L151, V33
G110, I13, S149, H154, S155, L152, L151
G1, N4, S99, H121, K97, I98, V2
G1, N4, S99, H121, K97, I91, V85
Epi#33
K32, F30, P35, S39, S57, K65
Q36, F30, P35, S39, S40, K32
K32, F30, P35, S40, S57, K65
K65, F58, P35, S39, A34, R145
Epi#34
V105, P14, T107, V12, R145, Y150, S155
I113, P14, T107, V12, R145, Y150, S155
Epi#37
P50, V74, L24, R17, N159
P50, V74, L24, K20, N159
P14, R17, L24, K20, N159
Epi#38
L143, G140, E141, R145, V33, N28, P31, S39
L143, G140, E141, R145, V33, N28, P31, S40
L143, G140, E141, R145, V33, N28, P31, S57
Epi#39
A130, E127, H126, T94, P90, G89, L62
A130, E127, H121, T94, P90, G89, L62
Epi#40
A157, L152, A153, Y150, K32, S39
A153, L152, A157, Y150, K32, S40
R17, L151, A153, Y150, K32, S40
R145, L143, A34, Y150, A153, S155
R145, L143, G140, T9, K115, T10
Epi#41
P63, Y66, L62, S57
Epi#44
I23, R17, D156, Y150, S149, V12, T10
L24, R17, D156, Y150, S149, V12, P14
L24, R17, D156, Y158, A16, A106, P108
I13, R17, D156, Y150, S149, V12, T10
L151, R17, D156, Y150, S149, V12, T10
L24, R17, D156, Y150, S149, V12, T107
Epi#45
K32, P35, F30, Y150, R145, M139, G140
K32, P35, F30, Y150, R145, M139, L143
K32, P31, F30, Y150, R145, M139, G140
Epi#47
L152, S149, R145, L143, A34, F30, N28, P31, P35
A153, S149, R145, A146, A34, F30, N28, P31, P35
Epi#48
E60, K65, P90, P63, G61
E60, K65, P63, P90, G92
Epi#51
T94, H126, E127, D125, G124, K123, H121
D125, H126, E127, T94, K123, T122, H121
Der f2:
Epi#02
A98, K100, S101, P99, R128, R31
A98, K100, R128, P99, R31, V94
T91, N93, P95, P34, R31, R128
L61, N93, P95, P34, R31, R128
Epi#03
L40, K15, A39, I13, Y86
L40, K14, A39, I88, Y90
Epi#05
G32, A98, R31, P34, G20, T36, T91, Y90
G32, A98, R31, P34, G20, T36, T91, V94
G32, A98, R31, P34, G20, T36, T91, L37
G32, A98, R31, P34, G20, T36, T91, V18
Epi#06
A98, P99, D129, R31, K96, P95
G32, P99, D129, R128, R31, P95
A98, P99, D129, R31, K33, P95
A98, P99, D129, R31, K96, P34
A98, P99, D129, R128, K126, P26
Epi#07
T107, S57, D59, S101, R128, A98, P99, D129
T107, S57, D59, S101, R31, A98, P99, D129
Epi#08
K15, D87, V76, H74, F75
K14, D87, V76, H74, F75
K77, D87, V76, H74, F75
Epi#09
L61, D64, I68, H74, F75, T70, N71
N114, N46, D113, K48, N71, T70, T49
G83, N46, D113, K48, N71, T70, T49
Epi#10
L40, I13, D42, N44, V81, K48, N46, N114, G115
L40, I13, D42, N44, V81, K82, N46, N114, G115
L37, D19, G20, V18, V3, D4, K6, A120, T107, V105
Epi#11
F75, K51, I111, Q45, V116, D113
F75, K51, I111, Q45, V81, D113
Epi#12
Y90, E38
Epi#13
H30, R31, P95, A98, P99, S101, G60, L61
Epi#15
[0561] K96, P99, D129, 128, R128, A98
K96, P99, D129, I127, R128, A98
K96, P99, D129, I29, R128, A98
K55, P66, D64, I68, T70, G67
Epi#18
R31, R128, I28, G125, T123, H124, V105
R31, R128, I127, G125, T123, H124, V105
Epi#22
D1, M17, D4, V3, K6
D1, M17, D19, P34, K96
D1, M17, D4, V5, K6
Epi#23
K14, N11, E12, N44, Q85, P79
K14, N11, E12, N10, Q45, P79
K14, N11, E12, N44, Q84, P79
K14, N11, E12, L40, Q85, P79
Epi#24
D129, K100, E102, P99, R128, R31, K96
E62, G60, E102, P99, R128, R31, K96
D129, K126, E102, P99, R128, R31, K33
D129, K126, E102, P99, R31, P95, K96
Epi#25
R31, K96, I97, D59, E62
R128, R31, I97, D59, E102
R128, K126, I127, E102, N103
Epi#27
D64, E62, D59, K100
D59, E62, D64, K55
D87, E38, D19, K33
D19, E38, D87, K15
D19, E38, D87, K14
D19, E38, D87, K77
Epi#28
V16, D87, Q85, K14, E12, K15, Q2, D1
I13, D87, Q85, K14, E12, K15, Q2, D1
V3, D1, Q2, K15, E12, K14, Q85, D87
L40, D87, Q85, K14, E12, K15, Q2, D1
I88, D87, Q85, K14, E12, K15, Q2, D1
V76, D87, Q85, K14, E12, K15, Q2, D1
V18, D1, Q2, K15, E12, K14, Q85, D87
Epi#29
G32, N93, L61, E62
V94, N93, L61, E62
Epi#30
G60, I97, A98, H30, K96, P34, P95
I68, N71, H74, K77, P79, V81
G32, I97, A98, H30, K96, P95, P34
Epi#34
V105, P26, S24, G125, R128, S101, P99
W92, P34, T91, V94, R31, S101, P99
I28, P26, T123, G125, R128, S101, P99
Epi#37
A120, V16, L40, K14, N11
A39, V16, L40, K14, N11
Y90, A39, L40, K14, N11
Y86, A39, L40, K14, N11
Epi#39
A120, E38, T91, P34, G20, L37
A39, E38, T91, P34, G20, L37
Epi#40
G20, L37, A120, T123, K6, S24
A39, L37, A120, T123, K6, S24
G20, L37, A120, T107, K6, T123
Epi#41
P34, L37, V106, S57
Epi#42
P26, S24, G125, R128, R31
P99, S101, G125, R128, R31
Epi#44
V16, Q2, D19, P34, W92, Y90, A39, V18, T91
V16, Q2, D19, P34, W92, Y90, A39, V5, T123
V3, Q2, D19, P34, W92, Y90, A39, V18, T91
Epi#45
K77, H74, F75, N71, D69, G67
[0562] K77, H74, F75, N71, D69, V76
K77, H74, F75, N71, D69, V65
Epi#46
A98, R128, R31, P95, N93, G32
A98, R128, R31, P34, G20, Q2
Epi#48
Q2, D19, P34, P95, G32
H30, K96, P95, P34, G20
Epi#49
D87, D42, L40, Q85, Q84, C78, T47, Q45, K48
D87, D42, L40, Q85, Q84, C78, T47, Q45, K82
Epi#50
D19, W92, P34, T91
D19, W92, P34, P95
D19, W92, T91, T36
Epi#51
D129, H30, K33, R31, R128, K126, H124
R31, H30, D129, R128, K100, K126, H124
T123, H124, K126, R128, R31, K33, H30
Der p2:
Epi#03
L17, K89, A39, I13, Y86
L17, K89, A72, I88, Y90
L17, K89, A72, I52, Y90
Epi#04
K15, S1, Q2, K14, V16, L17
[0563] K15, S1, Q2, K14, A39, L17
K15, S1, Q2, K14, V40, I13
Epi#05
G60, A56, L61, P99, G32, R31, H30, I97
G60, A56, L61, P99, G32, R31, H30, I28
Epi#06
G60, A56, D64, S57, K55, P66
G83, N46, D114, T49, K48, P79
G60, N103, D59, S101, R31, P95
Epi#08
K55, D64, S57, V106, F35
K55, E62, S57, V106, F35
Epi#09
L61, G60, E102, R128, I28, K126, N103, T123, V105
L61, G60, E102, R128, I127, K100, N103, T123, V105
L61, G60, E102, R128, I127, H124, N103, T123, V105
Epi#10
SAS: 435, Size 24.47: D69, T91, N93, F35, G32, R31
SAS: 422, Size 20.74: E38, T91, N93, F35, G32, K96
Epi#11
K14, I13, Q85, V81, E42
K15, I13, Q85, V81, E42
K14, I13, Q85, V40, D87
Epi#12
Y86, E42
Y90, E53
Y90, E38
Epi#13
H30, A125, P26, T123, A122, P19, L37, P34, W92
H30, A125, P26, T123, A122, H124, S24, G23, G20
H30, A125, P26, T123, A122, P19, L17, G20, F35
Epi#15
K55, P66, D69, I68, K89, A72
K55, P66, D69, I68, K89, A39
K55, P66, D64, I54, K109, G115
K55, P66, D64, I54, K109, A9
Epi#18
R31, I29, A125, S101, E102, N103
R31, I29, A125, S101, E102, V104
R31, I29, A125, T123, A122, V105
Epi#22
D69, P66, D64, V65, K55
D64, P66, D69, T91, K89
D59, L61, D64, P66, W92
D59, L61, D64, V65, E62
D69, P66, D64, V65, E53
Epi#24
D64, K55, E62, P99, R31, P34, K96
E53, K55, E62, P99, R31, P95, K96
D64, K55, E62, P99, R31, A98, K96
Epi#25
R31, H30, I28, E102, N103
R128, K126, I127, E102, N103
R128, K126, I28, E102, V105
Epi#27
D64, E53, D69, K89
D69, E53, D64, K55
D59, E62, D64, K55
Epi#28
V40, D87, Q85, E42, Q84, G83, K82
G20, H22, Q2, L17, E38, L37, Q36, P34, K33
G20, H22, Q2, L17, E38, L37, F35, P34, K33
Epi#29
I97, K100, L61, E62
G60, N103, L61, E62
I127, N103, L61, E62
Epi#30
G60, N103, S101, H30, K96, I97, P95
G60, N103, A125, H30, K96, I97, P95
I28, I127, A125, H30, K96, I97, P95
Epi#33
Q36, F35, V106, S57, A56, K55
K33, F35, V106, S57, A56, K55
Epi#34
I28, P26, S24, G23, G20, T123, S57
I28, P26, S24, V3, G20, T123, T107
W92, P34, T91, V18, G20, T123, P26
Epi#37
P66, V63, L61, K100, N103
P95, A98, L61, K100, N103
P19, V18, L17, K89, D87
P19, V3, L17, K89, D87
T123, V104, L61, K100, N103
Epi#38
L61, G60, E102, A125, V105, N103, P99, S57
L61, G60, E62, A56, V105, N103, P99, S57
Epi#39
A125, E102, H124, T123, P26, G20, L17
Epi#40
G60, L61, A56, T107, K6, T123
A39, L17, G20, T123, P26, S24
G60, L61, A56, T107, K55, S57
G60, L61, A56, T123, K126, S101
Epi#41
P19, L17, V3, S1
P19, L17, V5, S24
Epi#44
V65, D64, P66, W92, Y90, A39, V18, P19
L61, D64, P66, W92, Y90, A39, V18, T91
Epi#45
R31, P34, F35, N93, V94
K96, P34, F35, N93, G32
Epi#47
I127, S101, R31, I97, A98, L61, N103, P99, P95
I28, S101, R31, I97, A98, L61, N103, P99, S57
Epi#48
H30, K96, P95, P99, G60
H30, K96, P34, P19, G20
H30, K96, P34, P19, V18
H30, K96, P34, P95, V94
H30, K96, P34, P19, V3
E38, K89, P70, P66, V65
H30, K96, P95, P34, G32
Q36, K89, P70, P66, V65
Epi#50
D69, Y90, W92, P66, P70
D69, Y90, W92, P34, P95
D69, Y90, W92, T91, P34
D69, Y90, W92, V94, P95
D69, Y90, W92, L37, P19
Epi#51
K126, H124, E102, R128, I28, R31, H30
T123, H124, K126, R128, I28, R31, H30
D4, H124, K126, R128, I28, R31, H30
PhI p2:
Epi#02
T87, K85, Q61, S38, R34, R67
T87, K85, Q61, P63, R34, V42
Epi#03
K10, A90, I88, Y86
K10, A18, I88, Y86
Epi#04
R34, S38, Q61, K85, T87, I88
R34, S38, Q61, K85, T87, A90
Epi#05
G47, A18, S12, T87, G89, T91, T5, V1
G73, A29, L69, T27, G50, T53, T45, V42
G11, A18, L20, T91, G89, A90, T87, I88
Epi#06
A93, P94, D79, R34, Q61, P59
A93, P94, D79, R34, Q61, P83
A93, P94, D80, R34, Q61, P59
A93, P94, D79, R34, Q61, P63
Epi#08
K10, E9, G11, A18, H16, F54
K46, E48, G47, A18, H16, F54
K10, E9, S12, A18, H16, F54
Epi#09
L69, T27, G73, N76, R67, V77, D79, R34, A43, T45, V42
L69, T27, A29, E30, R67, V77, D80, R34, A43, T45, V42
Epi#10
D55, A18, N13, S12, F54, G47, K46
T45, A18, N13, S56, F54, G47, K46
Epi#09
L60, S56, E57, D55, K15, N13, S12, G11
L60, S56, E57, D55, H16, F54, T45, T53
L60, S56, E57, D55, H16, F54, T45, G47
Epi#12
Y86, E84
Y23, E24
Epi#18
N76, R67, F78, V81, A93, Y92, T91, T5, P2, V1
Epi#19
D39, W41, S38, Q61, R34, G37
E40, W41, S38, Q61, R34, A43
Epi#22
D79, P94, D80, P83, K85
D79, P94, D80, P63, K85
Epi#23
K10, N13, E14, L60, Q61, P59
K10, N13, E14, L60, Q61, P83
K10, N13, E14, L60, Q61, P63
Epi#24
E58, K15, E57, P59, S56, E14, Q61
D55, K15, E57, P59, S56, E58, Q61
Epi#25
R34, R67, W41, D39, E40
Epi#26
S38, E40, W41, V42, E32, E30
S38, E40, W41, V42, A43, E32
Epi#27
E14, E57, E58, K15
D55, E14, E84, K85
Epi#28
G37, H36, Q61, K85, E84, L60, F54, A43, K46
G37, H36, Q61, K85, E84, L60, F54, S12, D55
G37, H36, Q61, K85, E84, L60, F54, S56, D55
G37, H36, Q61, K85, E84, L60, F54, A43, R67
G37, H36, Q61, K15, E57, L60, F54, A43, K46
G37, H36, Q61, K85, E84, L60, F54, S12, K15
G37, H36, Q61, K85, E84, L60, F54, S56, K15
G37, H36, Q61, K85, E84, L60, F54, A43, R34
G37, H36, Q61, K85, E84, L60, F54, A18, D55
Epi#29
G73, K72, L69, R67, E30
I88, N13, L60, F54, E57
G25, K72, L69, R67, E32
V77, K75, L69, R67, E30
G37, H36, L60, F54, E57
G37, Q61, L60, F54, E57
Epi#30
I88, N13, S12, H16, K15, P59, L60
I88, N13, S56, H16, K15, L60, P59
I88, N13, A18, H16, K15, P59, L60
Epi#33
K46, F54, V42, S56, K15
H16, F54, V42, S56, K15
Epi#34
V1, P2, T5, V4, P94, Y92, T87
V1, P2, T5, L20, G89, T91, T87
V81, P94, T5, V1, P2, Y92, T91
Epi#37
T27, A29, L69, K72, D26
A43, R67, L69, K75, N76
Epi#38
L20, G89, E9, A18, N13, P59, S56
Epi#40
G49, L20, G89, Y86, K85, T87
G49, L20, G89, T87, K10, S12
G49, L20, G89, T87, K10, T7
Epi#44
V77, R67, D79, P94, Y92, A93, V1, P2
L69, R67, D79, P94, Y92, A93, V1, T5
Epi#45
D79, P94, F78, N76, M74, L69
D80, P94, F78, R67, D79, V77
K3, P94, F78, N76, M74, G73
Epi#46
A43, R67, R34, P63, H36, Q61
V77, R67, R34, P63, H36, G37
L69, R67, R34, P63, G37, Q61
Epi#47
G37, E35, E40, A43, R34, L60, N13, P59, S56
V77, E32, E40, A43, R34, L60, N13, P59, S56
S38, G37, E40, A43, R34, L60, N13, P59, S56
Epi#48
E24, K3, P94, P2, V1
E84, D80, P94, P2, V1
Epi#50
D39, W41, A43, T45
D39, W41, V42, T45
Epi#51
D79, H36, E84, T87, K10, G11, H16
D39, H36, Q61, K85, P63, R34, W41
D79, H36, E40, D39, G37, R34, W41
Q61, H36, E84, T87, K10, G11, H16
TABLE-US-00019 [0564] TABLE 8 Each row indicates an epitope
pattern. At each position (from 1 to maximum of 12) the cells
indicate which amino acids (single letter coding) are allowed at
that position. The last column indicates the patterns identified
using IgE antibody binding. Epitope Pattern Position Number 1 2 3 4
5 6 7 8 9 10 11 12 1 TS RQ YS NHC KR KR P HNP L IgE 2 RV R Y- PST
FR- ALPQS- RKN ALT IgE (SEQ ID NO: 197) 3 Y I AH- K L 4 AGIL ANRTV-
KRY Q S Y- KR (SEQ ID (SEQ ID NO: 198) NO: 199) 5 GILVY STH ASTR- G
PT- RNAFLS A G IgE (SEQ ID (SEQ ID (SEQ ID NO: 200) NO: 201) NO:
202) 6 P KRQSA STRC D PAN GA IgE (SEQ ID (SEQ ID NO: 203) NO: 204)
7 D P AV- R S- D S- T G 8 F HI- VA- FSG- DE- KA IgE 9 NRGLTV- STAN
ANF RKH D- AILV- R- (ENRSV- AGI- DGNT- LIS- IgE (SEQ ID (SEQ ID
(SEQ ID SEQ ID (SEQ ID NO: 205) NO: 206) NO: 207) NO: 208) NO: 209)
10 KR RG F C- AST- RN NTA DECT IgE (SEQ ID NO: 210) 11 DE V- Q I
FLK F 12 E Y IgE 13 FWYGL PG ALS- PH A T- P LRWA SAH IgE (SEQ ID
(SEQ ID NO: 211) NO: 212) 14 GV Q ILV I- Y GNR DN TEH 15 AG RKQT- I
D P RKN IgE (SEQ ID NO: 213) 16 DN A DA SDN QRSW GMR Y P RQL (SEQ
ID NO: 214) 17 S- R S A 18 VLSFN AEHNPT- T- L- ST- Y- GAL LIV- CSF-
R FRN- SD IgE (SEQ ID (SEQ ID NO: 215) NO: 216) 19 AGLKM R Q QSC
NTW DEI IgE (SEQ ID NO: 217) 20 D G D KN L LF- P K V A IgE 21 P S
I- I LR- CI IgE 22 EDKW ACLPTV D- ASLPM- D- IgE (SEQ ID WY- (SEQ ID
NO: 218) (SEQ ID NO: 220) NO: 219) 23 AP LQF SYLN- E N RK IgE (SEQ
ID NO: 221) 24 KQ AELFPR- TSFR P EA GK DE IgE (SEQ ID (SEQ ID NO:
222) NO: 223) 25 ENV DE IW- RKH R IgE 26 DE AGE PHV W E- S W IgE 27
K DE E DE IgE 28 DKR APSG- QF- CFIKLW- E FIKLW- Q DH- AGILV IgE
(SEQ ID (SEQ ID (SEQ ID (SEQ ID NO: 224) NO: 225) NO: 226) NO: 227)
29 E RF- L KRQHNG GILV IgE P (SEQ (SEQ ID ID NO: NO: 229) 228) 30
LVP LIP IKLPQS- H AS- LIMN GI (SEQ ID (SEQ ID NO: 230) NO: 231) 31
D FI- MV- FW R N QR L 32 V f- DE A A F 33 KR SA- S VP YF KQH IgE 34
STP STY GPR- GLV STM WP IVW 35 I M S A- L AG 36 AW A PV- K- Q- ST
Y- G- V- A A TP IgE 37 NYD KR L ARV TYAP IgE (SEQ ID NO: 232) 38 S
P N LR- RV- AR- E G L 39 L G P RT- HL- E A 40 ST- APK- YT AG L- AGR
IgE 41 St V- L Yh- P- 42 RQ R P- H- NQG S P L 43 T- RI ML S HQ GL
YA WC I 44 PT AGV SA Y W- P- D- RQ- ILVS IgE (SEQ ID NO: 233) 45
LVG MD- RN Y- F PH KRD IgE 46 AGQ HNQGC P R R AVLCY IgE (SEQ ID
(SEQ ID NO: 234) NO: 235) 47 PS RP N LFQA- AR AILMNV RE AGSYLE
LIAGVS (SEQ ID (SEQ ID (SEQ ID (SEQ ID NO: 236) NO: 237) NO: 238)
NO: 239) 48 GV P P KHQD SHQE (SEQ ID (SEQ ID NO: 240) NO: 241) 49
KN Q- TMC WYC- Q Q FP- VP- L W- D 50 PST STAPLW W WY- RHD V (SEQ ID
NO: 242) 51 WH TSKHRQ LIRKGP DSRTQG DEKQHT H RKQDT G (SEQ (SEQ ID
KH- (SEQ (SEQ ID (SEQ ID ID NO: NO: 244) ID NO: NO: 246) NO: 247)
243) 245) 52 Q DNT- W R STRE- A FW (SEQ ID NO: 248)
Example 10
[0565] For this example the third-generation epitope sequences were
determined in further 11 environmental allergens (Bosd2, Equc1,
Gald4-mutant (with alanine substituted for glycine in position
102), Hevb8, Profillin1-AC, Profillin1-AT, Profillin2-AC,
Profillin-birch pollen, Rag weed pollen5 and Vesv5), based on their
structures sequences (SEQ ID NOS: 12, 13, 15, 16, 17, 18, 19, 20,
21 and 22, respectively), their structures (1bj7.pdb, 1ew3.pdb,
1flu.pdb, 1g5u.pdb, 1prq.pdb, 1a0k.pdb, 1f2k.pdb, 1cqa.pdb,
1bbg.pdb, and 1qnx.pdb, respectively), and computer modelling of
the epitope patterns that had been assembled in our database (shown
in Table 8). Further, the epitope sequences of the four
environmental allergens of example 9, Bet v1, Der f2, Der p2, and
PhI p2, were redetermined.
[0566] The additional allergens arise from common sources of
allergy: cows (Bos d2 which is a bovine member of the lipocalin
family of allergens), horses (Equ C1, a major horse allergen also
of the lipocalin family), Hen egg white (Lysozyme Gal D 4), Latex
(Hey b8, a profilin from Hevea brasiliensis), Acanthamoeba
castellani (Profilin1-AC, a profilin isoform IA and Profilin2-AC, a
profilin isoform II), Arabidosis thaliana (Profillin1-AT a
cytoskeleton profilin), Birch (Profilin-birch pollen (Birch pollen
profilin), Rag weed pollen5 (Ragweed pollen allergen V from
Ambrosia trifida) and whasp venom (Ves v5 allergen from Vespula
vulgaris venom).
[0567] The protein surface is scanned for epitope patterns matching
the given "consensus" sequence of about 6-12 residues. First,
residues on the protein surface that match the first residue of the
consensus sequence are identified. Within a specified distance from
each of these, residues on the protein surface that match the next
residue of the consensus sequence are identified. This procedure is
repeated for the remaining residues of the consensus sequence. The
method is further described under the paragraph "Methods" above and
the program can be found in Appendixes.
[0568] The critical parameters used in this screening included:
[0569] i) a maximal distance between the alpha-carbon atoms of
subsequent amino acids, [0570] ii) a minimal accessability of the
amino acid of 20 .ANG.2, [0571] iii) the largest maximal distance
between the most distinct amino acids should be less than 25 .ANG.,
[0572] iv) the best epitope were taken, [0573] v) the homology with
the epitope pattern of interest was 100%
[0574] In this way a number of potential epitopes are identified.
The epitopes are sorted according to total surface accessible area,
and certain entries removed: [0575] a. Epitopes that contain the
same protein surface residue more than once. These are artefacts
generated by the described algorithm. [0576] b. Epitopes which are
"too big", i.e. where a distance between any two residues in the
epitope exceeds a given threshold.
[0577] The epitope sequences found were:
Bosd2:
Epi#01
L65, P155, P156, R17, R40, N37, Y39, R41, T67
L65, P155, P156, R17, R40, N37, Y39, R41, S52
L64, P155, P156, R17, R40, N37, Y39, R41, T54
Epi#02
T121, K150, S122, R17, P156, Y39, R41, R40
T121, K150, S122, R17, P156, Y56, R36, V30
Epi#03
L128, K130, H92,17, Y76
L134, K130, H92,17, Y76
L128, K130, H92,191, Y76
Epi#04
R72, Y76, S50, Q73, K71, V69, I45
K71, Y76, S50, Q73, R72, V69, L80
K71, Y76, S50, Q73, R72, V69, I42
Epi#06
G14, P13, D47, S10, K11, P9
G14, P13, D47, S10, S94, P9
G14, P13, D47, C44, S10, P9
Epi#08
K71, E49, S50, V69, F82
K71, E49, S50, V79, F82
Epi#09
I7, S10, D8, E95, K119, N96, S122, T121
S10, I7, D8, E95, K11, N96, S122, T124
Epi#10
E15, T54, R41, T67, F55, R17, K119
E43, T54, R41, T67, F55, R17, K119
E31, T151, N153, C63, F55, R40, R41
E31, T151, N153, C154, F55, R41, R17
Epi#11
K26, I145, Q132, E143
K26, I145, Q132, E137
K26, I145, Q132, E129
Epi#12
Y105, E108
Y83, E81
Epi#15
N153, P156, D152, I149, T121, G120
R17, P156, D152, I149, T121, G120
N153, P156, D152, I149, R17, G14
Epi#18
R109, 1110, G107, Y83, T85, E81, V69
R109, 1110, G107, Y105, T85, E81, V69
Epi#19
E43, N46, S50, Q73, R72, K71
D47, N46, S50, Q73, R72, G75
E49, N46, S50, Q73, R72, K71
I45, N46, S50, Q73, R72, K71
Epi#20
V30, K28, P34, L57, L65, K58, D59, G32, D27
V30, K28, P34, L57, L64, K58, D59, G33, D27
Epi#22
D8, S10, D47, P13, E15
D8, S10, D47, P13, E43
D47, S10, D8, V93, E95
D8, S10, D47, C48, K71
Epi#23
K119, N96, E127, S122, L128, P125
K150, N147, E146, Y20, F123, P125
K11, N96, E127, S122, L128, P125
Epi#24
E129, K130, E126, P125, S122, L128, Q133
E126, K130, E129, P125, S122, R17, K119
E126, K130, E129, P125, T124, L128, Q133
Epi#25
R72, K71, I45, D47, N46
R72, K71, I45, E43, N46
Epi#27
D47, E49, E74, K71
D24, E143, E146, K150
D47, E43, E15, K119
Epi#28
L134, Q133, L128, E126, K130, F123, S122, K150
Q132, K130, E126, L128, F123, S122, K150
L65, D59, Q60, K58, E31, L57, G32, D27
G61, D59, Q60, K58, E31, K28, G32, D27
Epi#29
V69, K71, L80, R72, E74
I45, K71, L80, R72, E74
G61, Q60, L64, F55, E68
Epi#30
G120, N96, S94, H92, K130, L128, P125
I91, I7, S94, H92, K130, L128, P125
Epi#33
K130, F123, P125, S122, K150
K71, Y76, P9, S10, S94, K119
Epi#34
I7, P9, S10, G14, R17, T121, S122
I45, P13, S10, G14, R41, Y39, P156
Epi#37
T67, V69, L80, K71, Y76
P156, R40, L65, K58, D59
P155, R40, L65, K58, N153
Epi#38
L80, G84, E108, R109, N25, P141, S136
Epi#39
E137, R138, P141, G139, L134
E31, L57, R36, P34, G84, L80
Epi#40
R17, G120, T121, K150, S122
R17, G120, T121, K150, T151
Epi#41
P34, Y83, L80, V69, S52
P34, Y83, L80, V79, S50
Epi#42
L128, P125, S122, G120, R17, R41
L128, P125, S122, G120, R17, R40
Epi#44
S10, D47, P9, Y76, S50, V69, T67
I45, D47, P9, Y76, S50, V69, T67
Epi#45
D27, P34, F82, Y105, R109, D106, G107
D59, P34, F82, Y105, R109, D106, G107
K58, P34, F82, Y105, R109, D106, G107
D27, P34, F82, Y105, R109, D106, G84
Epi#46
Y39, R41, R40, P155, C63, Q60
Y20, R17, R40, P155, C63, Q60
Epi#47
L128, E126, E129, L134, R138, Q133, N142, P141, S136
V69, E81, E68, I42, R41, F55, N37, R40, P156
V69, E43, E15, I42, R41, F55, N37, R40, P156
S122, E127, E129, L134, R138, Q133, N142, P141, S136
Epi#48
E43, D47, P13, P9, V93
S10, D47, P9, P13, G14
E43, D47, P13, P9, V90
E49, D47, P13, P9, V93
Equc1:
Epi#02
L66, N68, A65, F90, S69, Y72, R64, V89
A65, R64, S31, F28, S112, Y123, R110, V108
L179, R180, Q178, F177, P143, Y38, R141, V145
L66, R64, S31, F28, S112, Y123, R110, V125
L66, N68, A65, F90, S69, Y72, R64, V62
Epi#03
K32, A65, I63, Y72
Epi#05
G35, A65, S69, T93, G97, R26, S112, Y123
G35, A65, S69, T93, G97, R26, S112, I25
Epi#07
G97, T93, S70, D91, S100, R110, V125, P132, D128
Epi#08
K129, D130, F127, V108, F90
K129, D130, F127, V108, F109
K129, D130, F127, V125, F136
K129, D130, F127, V125, F133
Epi#10
E48, N53, N80, T77, C83, F177, R175, K172
E82, N80, N53, T77, C83, F177, G181, R180
E52, N53, N80, T77, C83, F177, R175, K172
Epi#11
F133, K47, I167, Q158, V163, E165
Epi#12
Y38, E142
Y38, E36
Y139, E142
Epi#13
K129, P132, D45, 1167, Q158, G161
R131, P132, D45, 1167, K164, G161
Epi#16
P87, Y72, R64, S70, S69, D67, A65, N68
Epi#17
A65, S31, R64, S34
Epi#18
R64, S31, I30, A65, S34, L66, N68, S69
Epi#19
E82, N80, C83, Q178, R175, K172
Epi#22
D130, P132, D128, Y106, K129
Epi#23
D144, K150, E148, P147, S146, E151, K155
Epi#25
R160, K159, I156, E151, E148
Epi#27
E118, E142, D144, K172
E36, E142, D144, K172
Epi#28
I173, D174, Q178, L179, E85, C83, F177, G181, R180
I173, D174, Q178, L179, E85, C83, F177, P143, D144
Epi#29
G181, Q178, L179, R180, E36
G181, Q178, L179, R180, E85
Epi#30
I30, N27, S112, H119, I121, I25, V23
Epi#31
L122, R110, N27, R26, F28, I30, D96
L124, R110, N27, R26, F28, I30, D96
Epi#33
H119, Y38, V62, S34, S31, R64
Epi#34
V62, P87, M88, V89, R64, S31, S34
Epi#37
P87, V89, L66, R64, D67
Epi#40
R64, L66, A65, Y72, S34
R64, L66, A65, Y72, S69
Epi#41
P132, Y106, L101, V89, S100
P132, Y106, L101, V89, S70
Epi#44
V46, R131, D128, P132, Y106, S100, V89, P87
Epi#45
K129, P132, F127, Y106, N102, D91, V89
K129, P132, F127, Y106, N102, D104, G105
Epi#47
S146, E148, E152, V23, R26, A24, N27, R110, S112
V23, E115, E118, N116, R26, F28, N27, R110, S112
Gald4:
Epi#01
L75, N65, P70, R73, R61, N59, Y53, R45, T47
L75, N65, P70, R68, R61, N59, Y53, R45, T47
Epi#02
A90, N77, L75, R73, P70, R61, R68
A122, R125, Q121, T118, R114, R112
Epi#04
R21, Y20, S24, Q121, R125, R128, L129
R21, Y20, S24, Q121, R125, R128, G126
Epi#05
G16, A10, R128, G126, A122, T118, G117
G4, A10, R128, G126, A122, T118, G117
Epi#06
G67, P79, D66, R61, R73, P70
G67, N65, D66, S72, R73, P70
G49, N46, D48, R61, R73, P70
Epi#07
G71, T69, D66, S72, R73, P70, D48
G67, T69, D66, S72, R73, P70, D48
Epi#08
K1, D87, S86, V2, F38
K1, D87, S86, V2, F3
Epi#09
Epi#10
E7, A11, R14, A10, C6, F3, R5, R125
D87, A11, R14, A10, C6, F3, R5, R125
T47, N46, N44, S36, F34, R114, R112
D18, A10, R14, A11, C6, F3, R5, R125
T118, N113, R112, A110, F34, R114, K116
Epi#11
L129, I124, Q121, V120, D119
Epi#12
Y53, E35
Epi#15
R73, P70, D66, I78, A82
R73, P70, D66, I78, A90
Epi#17
A102, S100, R21, S24
Epi#18
R112, N113, R114, F34, V109, A107, A102, N103
N113, R112, R114, F34, V109, A107, N103, S100
Epi#19
D18, N19, S24, Q121, R125, L129
D18, N19, S24, Q121, R125, G126
Epi#22
D48, P70, D66, W63, W62
D66, P70, D48, T69, W62
D48, P70, D66, W63, K97
Epi#23
R45, N44, E35, N39, Q41, A42
R45, N44, E35, Y53, Q41, A42
Epi#25
R128, R125, W123, D119, N27
R128, R125, W123, D119, V120
Epi#26
W62, S72, W63, P79, A82, D87
W62, S72, W63, P79, G67, D66
Epi#28
G117, D119, Q121, I124, E7, C6, F3, A11, R14
A122, D119, Q121, I124, E7, C6, F3, A11, R14
Epi#29
G126, R125, L129, R128, E7
G16, R14, L129, R128, E7
Epi#30
I124, L129, A10, H15, I88, L84
I124, L129, A11, H15,188, L84
Epi#31
L75, R73, N65, R61, W62, I98, D101
L75, R73, N74, R61, W62, I98, D101
Epi#33
Q41, F38, V2, S86, S85, K1
Q41, F38, V2, S36, A110, R114
Epi#34
W63, W62, T69, G71, R73, S72, P70
W62, W63, S72, L75, R73, T69, P70
Epi#36
A110, A107, A102, S100, K96, A90, A82
Epi#37
A10, R128, L129, R14, D18
A10, R128, L129, K13, N19
Epi#40
R128, L129, A11, T89, A90, S85
R14, L129, A11, T89, A90, S85
Epi#41
Y53, L84, S81
Y53, L84, S86
Epi#42
P79, S81, N65, P70, R61, R73
P79, S81, N65, P70, R61, R68
Epi#44
L129, R14, D18, Y20, S24, V120, T118
L129, R14, D18, Y23, S24, V120, T118
Epi#46
L75, R61, R73, P70, N65, G67
L75, R73, R61, P70, N65, A82
L75, R61, R68, P70, N65, G67
Epi#47
S72, G71, R68, N65, R61, L75, N77, R73, P70
G67, S72, R68, N65, R61, L75, N77, R73, P70
Epi#49
D87, L84, Q41, Q57, Y53, T43, N44
D87, L84, Q57, Q41, Y53, T43, N46
D87, L84, Q41, Q57, Y53, T43, N39
Epi#50
R73, W62, W63, P79, S81
R73, W63, W62, S72, P70
Epi#51
D18, H15, K13, R14, L129, R125, W123
Epi#52
F34, A110, R112, R114, W111, N27, Q121
F3, A11, E7, R5, W123, D119, Q121
W123, A122, T118, R114, W111, N27, Q121
Hevb8:
Epi#01
L20, P109, P112, K86, R84, N116, Y125, Q129, T111
L110, P109, P112, K86, R84, N116, Y125, Q129, T111
Epi#02
A48, K43, Q41, F42, T70, Y72, R84, V74
T21, R19, P109, P112, R84, V74
A49, K43, Q41, F42, T70, Y72, R84, V74
Epi#03
L65, K86, I75, Y72
Epi#05
G30, A48, L60, P62, G58, T63, H66, G69
G58, A61, R84, P112, G113, T111, S89, G88
G80, A81, F54, P79, G58, T63, H66, G69
G77, A81, F54, P79, G58, T63, H66, G69
Epi#06
G58, P79, D55, S59, K52, P57
G80, P79, D55, S59, K52, P57
G77, P79, D55, S59, K52, P57
Epi#07
G17, T5, S2, D16, R19, P109, D107
Epi#08
K52, D45, S44, A49, H66, F42
Epi#10
E78, A81, R96, F54, G58, K52
D55, A81, R96, F54, G80, K52
Epi#11
F54, L60, I83, Q76, V82, E78
Epi#12
Y106, E108
Epi#13
H66, L65, P62, T63, A61, P57, A81, P79, G58
H66, L65, P62, T63, A61, P57, A81, P79, G80
H66, L60, P62, T63, A61, P57, A81, P79, G77
Epi#15
R19, P109, D107, I105, K86, G88
Epi#18
R19, G17, P109, S89
Epi#22
D29, S44, D45, A48, K52
D29, M51, D55, P79, E56
D45, M51, D55, P79, E78
D29, S44, D45, A49, K52
D45, M51, D55, P79, E56
D29, M51, D55, P57, E78
D29, M51, D55, P57, E56
D45, M51, D55, P57, K52
D45, M51, D55, P57, E78
Epi#24
D55, K52, E56, P79, F54, E78, Q76
D45, K52, E56, P57, F54, E78, Q76
Epi#25
R84, K86, I105, D107, E108
R96, H28, I26, D29, V3
Epi#26
W33, S2, W3, V32, G30, D29
Epi#27
D53, E56, D55, K52
Epi#28
V32, Q41, K43, E46, K52, F54, P57, D55
G69, Q41, K43, E46, K52, F54, P57, D55
Epi#29
G130, Q99, L127, R96, E78
L127, Q99, L131, R96, E78
G98, Q99, L127, R96, E78
Epi#30
G69, L67, A49, H66, K71, L65, P62
G80, M51, A48, H28, Q99, L127, L131
Epi#33
Q41, F42, V32, S31, S44, K43
Q41, F42, V47, S44, A48, K52
Q41, F42, V47, S44, A49, K52
Epi#34
I105, P112, S89, L110, R19, T21, S37
I105, P112, T111, L20, R19, T21, S37
Epi#37
T63, A49, L60, K52, D55
P62, V74, L60, K52, D45
P62, A61, L60, K52, D55
Epi#38
G77, E78, R96, V82, R84, N116, P112, S89
Epi#39
A48, E46, H66, T63, P62, G58, L60
A49, E46, H66, T63, P62, G58, L60
Epi#40
R19, L110, G113, T111, P109, S89
R19, L110, G113, T111, P112, S89
Epi#41
P62, L65, V47, S44
P109, Y106, L110, S89
P112, Y106, L110, S8
Epi#44
L20, R19, D16, W3, Y6, S2, G17, P109
L110, R19, D16, W3, Y6, S2, G17, P109
Epi#45
K52, P57, F54, R96, D124, L127
D55, P79, F54, R96, D124, L131
Epi#47
I75, G77, E78, V82, R84, N116, P112, S89
I75, G77, E78, I83, R84, N116, P112, P109
Epi#48
E78, Q76, P79, P57, G58
E78, Q76, P79, P57, G80
E78, Q76, P79, P57, G77
Epi#50
D9, W3, W33, S2, T5
D16, W3, W33, S2, T5
Epi#51
R19, H18, E108, S89, K87, K71, H66
R19, H18, E108, D107, K87, K71, H66
Profillin1-AC:
Epi#01
L116, N111, P106, K80, K81, N101, S83, Q105, T108
L116, N111, P106, K80, K81, N101, Y100, Q105, S83
Epi#02
T44, N51, P54, R56, T69, Y78, R71, V68
L24, K93, S92, R75, S76, Y78, R71, R56
Epi#03
L24, K93, I121, Y119
L24, K90, I121, Y119
Epi#04
K80, Y100, S83, Q105, K103, N101, G82
K80, Y100, S83, Q105, K103, T17, G12
K80, Y100, S83, Q105, K103, T17, G14
Epi#05
G34, A33, A36, T38, G64, A63, H66, V68
G34, A33, S32, T17, G12, T4, S1, Y5
Epi#06
A46, N50, D53, R56, A57, P54
A52, N50, D53, R56, A57, P54
A72, N50, D53, R56, A57, P54
A57, P54, D53, S47, Q43, P39
Epi#07
G64, T38, D61, S58, R56, A57, P54, D53
G64, T38, D61, S58, R56, A52, P54, D53
Epi#08
K103, E102, G82, V68, H66, F60
K81, E102, G82, V68, H66, F60
Epi#09
L24, S47, D53, A57, V68, R71, L70, R56, N51, N50, R75
L24, S47, D53, A57, V68, R71, L70, R56, N51, T44, T38
Epi#10
D74, N50, N51, T44, F60, R56, R71
D53, N50, N51, T44, F60, R56, R71
Epi#11
F125, K93, I121, Q123, D118
F125, K90, I121, Q123, D118
F49, K90, I121, Q123, D118
Epi#12
Y119, E114
Y100, E102
Epi#13
A57, R56, P54, T44, A40, P39, A36, G64, Y67
S58, A57, P54, T44, A40, P39, A36, G64, Y67
Epi#15
N51, P54, D53, I55, R56, A57
R56, P54, D53, I55, T69, A57
R56, P54, D53, I55, T44, A40
Epi#16
Q105, P106, Y100, G14, Q18, S32, A36, A33, D7
Q105, P106, Y100, G14, Q18, S32, A36, A63, D61
Epi#17
A110, S76, R75, S92
A72, S76, R75, S92
Epi#18
N51, N50, R75, S92, L24, S47, T44, P39, N27
N51, N50, R75, S92, L24, T28, T38, P39, N27
Epi#22
D53, S47, D25, L24, K93
D53, S58, D61, V68, K81
Epi#23
K103, N101, E102, S83, Q105, P106
K103, N101, E102, S83, Q105, A84
Epi#24
E114, K115, A110, P106, S83, E102, K103
D53, G59, A57, P54, R56, L70, K80
E102, K103, A15, P106, S83, A84, Q105
Epi#25
R71, R56, 155, D53, N50
R71, R56, 155, D53, N51
Epi#28
I104, Q105, K103, E102, K81, S83, K80
G107, Q105, K103, E102, K81, G82, K80
A84, Q105, K103, E102, K81, S83, K80
A110, Q105, K103, E102, K81, S83, K80
Epi#29
I121, K115, L116, E114
V112, K115, L116, E114
Epi#30
G59, I55, S58, H66, K80, L70, V68
G59, I55, S58, H66, K80, P106, V99
Epi#33
K80, Y78, V68, S58, A57, R56
K81, Y67, V68, S58, A57, R56
Epi#34
I55, P54, S58, V68, R71, Y78, P106
W29, W2, T4, V11, G12, Y5, S1
Epi#36
A63, A36, A33, V11, G14, Y100, S83, Q105, K103, P106, A110, A15
A63, A36, A33, V11, G14, Y100, T108, Q105, K103, P106, A15,
A110
Epi#37
A57, R56, L70, R71, Y78
A57, V68, L70, R56, D53
Y78, R71, L70, R56, N51
P54, R56, L70, R71, D73
T69, R71, L70, R56, D53
Epi#38
G82, E102, A84, V99, N101, P106, S83
Epi#40
R71, L70, A72, Y78, K80, S83
R71, L70, G59, T69, K81, S83
R56, L70, A72, T69, K81, S83
Epi#41
P106, Y78, L70, V68, S58
Epi#42
P54, S47, N51, R56, R71
P54, S58, G59, R56, R71
Epi#44
S83, Q105, P106, Y78, A110, G107, T108
V68, R71, D73, Y78, A110, G107, T108
L70, R71, D73, Y78, A110, V112, T108
L70, R71, D73, Y78, A110, G107, P106
Epi#45
K81, H66, F60, R56, D53, G59
K80, H66, F60, R56, D53, G59
D61, H66, F60, R56, D53, G59
Epi#46
L70, R71, R56, P54, N51, A52
L70, R71, R56, P54, N51, A72
V68, R71, R56, P54, N51, A46
Y78, R71, R56, P54, G59, A57
Epi#47
V68, A57, R56, L70, R71, A52, N51, P54, S58
S58, A57, R56, L70, R71, A72, N51, P54, S47
Epi#49
D25, L24, Q43, Q41, T44, N51
D25, L24, Q43, Q41, T38, N27
Epi#50
D7, W2, W29, S1, T4
D7, Y5, W2, W29, S1
Epi#51
K80, H66, D61, T44, P39, T28, W29
K80, H66, D61, T38, P39, T28, W29
Profillin 1-AT:
Epi#01
P109, P89, K86, R84, N116, Y106, Q114, T111
Epi#02
L42, K43, Q45, F66, T63, Y72, R84, V74
L42, K43, Q45, F66, T63, Y72, R84, V82
Epi#03
K96, I127, Y125
K86, I75, Y72
Epi#05
G77, A81, F54, P57, G58, A61, T63, V74
G58, A61, F59, P57, G77, A81, T97, G80
G80, A81, F54, P57, G58, A61, T63, Y72
Epi#06
G17, P109, D107, T21, K38, P40
G112, P109, D107, T21, K38, P40
G88, P89, D107, T21, K38, P40
Epi#08
K52, E55, G58, V74, F66
K51, E55, G58, A61, F59
Epi#09
D29, D48, K52, F59, A61, T63
D29, D48, K51, F59, A61, T63
Epi#10
E108, T111, N18, T21, F39, G68, K71
E108, T111, N18, T21, F105, G112, K86
Epi#11
F105, K86, I75, Q76, V82, E78
F66, K43, I47, Q28, V32, D29
F59, K52, I47, Q28, V32, D29
Epi#12
Y125, E130
Y125, E128
Epi#15
K43, P44, D29, I47, K52, G58
K43, P44, D48, I47, Q45, G49
K43, P44, D29, I47, K51, G80
Epi#20
K38, P40, F39, L42, K43, D48, G30, D29
K51, P57, F59, L60, K52, D48, G30, D29
Epi#22
D48, P44, D29, V32, W33
D48, P44, D29, V32, W3
Epi#24
D29, K51, E56, P57, F59, E55, Q79
D48, K52, E55, P57, F59, E56, Q79
Epi#25
R121, K95, I83, D53, E55
R121, K95, I83, E78, V82
Epi#26
W33, S2, W3, V32, G30, D29
Epi#27
E128, E130, D124, K96
E130, E128, D124, K95
Epi#28
I75, Q76, E78, Q79, P57, K51
A61, Q76, E78, Q79, P57, K52
V32, D29, Q99, E130, I127, S129, D124
V32, D29, Q99, 1127, E128, S129, D124
Epi#29
V32, Q41, L42, F66, E70
G69, Q41, L42, F66, E70
G68, Q41, L42, F66, E70
Epi#30
G17, N18, H19, Q114, L117, V15
G17, M110, H19, Q114, L117, V15
G113, M110, H19, Q114, L117, V15
Epi#33
Q41, F39, P40, S36, A37, K38
Epi#34
V74, P62, M73, G88, P89, Y106, T111
Epi#37
T111, V15, L117, R121, Y125
T111, V15, L117, R121, D124
Epi#39
A81, E55, P57, G58, L60
A81, E78, P57, G58, L60
Epi#40
R121, L117, G112, Y106, P109, T111
R121, L117, G112, Y106, P89, T111
Epi#41
Y125, L131, S129
Epi#44
I75, R84, Y72, A61, G58, P62
I75, R84, Y72, A61, V74, T63
Epi#45
K38, P40, F105, Y106, N18, D14, G17
K38, P40, F105, Y106, N18, D107, G88
K38, P40, F105, Y106, N18, D14, V15
Epi#48
E16, H19, P109, P89, G88
E16, H19, P109, P89, G112
Epi#49
D124, L131, Q99, Q28, T97, N98
D124, L131, Q99, Q28, T97, K96
Epi#50
D9, Y6, W3, W33, S2
D9, W3, W33, S2, S5
D9, W3, W33, V32, S31
Epi#51
D14, H19, E108, T111, L117, R121, H10
D107, H19, E16, Q114, L117, R121, H10
D14, H19, D107, T21, K38, Q35, W33
Profillin2-AC:
Epi#01
L116, N111, P106, K80, K81, N101, S83, Q105, T108
L116, N111, P106, K80, K81, N101, S83, Q105, T108
Epi#02
T53, N58, S57, R56, T69, Y67, R66, V68
T53, K50, A52, R56, T69, Y67, R66, V68
T53, K50, A72, R56, T69, Y67, R66, V68
Epi#03
L116, K115, I121, Y119
Epi#04
K81, Y100, S83, Q105, K103, T17, G12
K80, Y100, S83, Q105, K103, A84, G82
K81, Y100, S83, Q105, K103, T17, G14
K80, Y100, S83, Q105, K103, N101, I104
K81, Y100, S83, Q105, K103, A15, G107
Epi#06
A54, N47, D25, T28, A36, P39
A40, N27, D25, T28, A36, P39
A44, N47, D25, T28, A36, P39
G34, A33, D7, T31, A36, P39
A43, N47, D25, T28, A36, P39
Epi#08
K103, E102, G82, V68, F60
K103, E102, G82, V68, F60
K81, E102, G82, V68, F60
Epi#10
T53, N58, R56, S57, F60, R66, K81
E61, N58, R56, S57, F60, R66, K80
Epi#11
F125, K93, I121, Q105, E102
F125, K93, I121, Q123, D118
Epi#12
Y100, E102
Y119, E114
Epi#13
A52, A44, P39, A43, H24, S92, G124, Y119
A46, A44, P39, A43, H24, S92, G124, Y119
Epi#15
K103, P106, D118, I121, K93, G124
K103, P106, D118, I121, Q105, G107
K103, P106, D118, I121, Q123, G122
Epi#16
Q105, P106, Y78, R71, S57, N58, A54, A44, D51
Q105, P106, Y78, R71, R56, D51, D74, A52, N47
Epi#18
R66, N58, R56, S57, V68, G82, S83, E102, N101
R66, N58, R56, S57, V68, G82, S83, P106, N101
Epi#22
D74, A52, D51, T53, K50
D25, A44, D51, T53, K50
D74, A46, D51, T53, K50
D74, A72, D51, T53, K50
Epi#23
K103, N101, E102, S83, Q105, P106
K103, N101, E102, S83, Q105, A84
Epi#24
D74, K81, A84, P106, S83, E102, K103
D74, K81, E102, P106, T108, A15, K103
Epi#25
R66, K81, E102, N101
Epi#28
I121, D118, Q105, K103, E102, K81, G82, D74
G107, D118, Q105, K103, E102, K81, G82, D74
G122, D118, Q105, K103, E102, K81, G82, D74
Epi#29
I121, K115, L116, E114
V112, K115, L116, E114
Epi#30
I55, N47, A44, H24, K93, I121, L116
I55, N47, A43, H24, K93, I121, L116
Epi#31
R56, N58, R66, F60, V68, I55, D51
R66, N58, R56, F60, V68, I55, D51
Epi#33
K115, Y119, P106, S83, A84, K103
Q123, Y119, P106, S83, A84, K103
K81, Y67, V68, S57, A54, R56
K80, Y78, V68, S57, A54, R56
Epi#34
W29, W2, T8, V11, G12, T4, S1
W29, W2, T4, G12, G14, T13, T8
Epi#37
T108, V112, L116, K115, Y119
T108, A110, L116, K115, N111
T13, V112, L116, K115, D118
P106, A110, L116, K115, N111
Epi#38
G64, E61, A40, V37, N27, P39, S38
G82, E102, A84, V99, N101, P106, S83
Epi#39
A110, E114, T108, P106, G122, L116
Epi#40
G14, G12, T17, K103, S83
R56, A52, T53, A54, S57
R66, A63, T65, K81, S83
R56, A72, T53, A54, S57
R56, G59, T53, A54, S57
R66, G64, Y67, K81, S83
Epi#42
P106, S83, G82, R75, R71
Epi#44
S1, Q3, D7, W2, Y5, S32, G12, T8
S1, Q3, D7, W2, Y5, A30, A36, P39
S1, Q3, D7, W2, Y5, S32, V11, T8
S1, Q3, D7, W2, Y5, S32, G12, T4
S1, Q3, D7, W2, Y5, A30, A33, T31
S1, Q3, D7, W2, Y5, A30, A36, T28
S1, Q3, D7, W2, Y5, S32, G12, T13
S1, Q3, D7, W2, Y5, S32, G34, T31
Epi#45
K93, H24, F49, R75, D74, G82
D25, H24, F49, R75, D74, G82
Epi#47
A36, G64, E61, A40, A44, A54, N58, R56, S57
Epi#50
D7, Y5, W2, T8, S1
D7, W2, W29, T28, P39
Epi#51
K90, H24, K93, D25, P39, T28, W29
T91, H24, K93, D25, P39, T28, W29
Profillin-Brich Pollen:
Epi#01
L124, N118, P114, K88, K73, H68, Y74, R86, T95
Epi#02
T113, N118, Q116, P114, R86, V76
T50, K54, L62, T65, Y74, R86, V84
Epi#03
L133, K98, I129, Y127
Epi#04
S40, Q43, K45, T50, G32
S40, Q43, K45, T50, G51
S40, Q43, K45, T50, I49
Epi#05
G82, A81, A83, P59, G60, A63, T65, V76
G82, A83, A81, P59, G60, A63, H61, V76
G79, A81, A83, P59, G60, A63, T65, V76
Epi#06
G70, P46, D31, T50, K54, P59
A81, P59, D55, T50, Q47, P46
G32, P46, D31, T50, K45, P42
G51, P46, D31, T50, K54, P59
Epi#08
A81, E57, G60, A63, H61, F56
A81, E57, G60, V76, H68, F44
K54, E57, G60, A63, H61, F56
Epi#11
F56, K98, I85, Q78, V84, E122
F56, K98, I27, Q37, V34, D31
F56, K97, I85, Q78, V84, E80
Epi#12
Y6, E9
Y127, E122
Epi#13
H68, L62, P64, T65, A63, P59, A81, G82, G79
H61, L62, P64, T65, A63, P59, A81, G79, F56
H68, L62, P64, T65, A63, P59, A83, G79, G60
Epi#15
K45, P46, D31, I49, Q47, G32
K45, P46, D31, I49, K54, G60
K45, P46, D31, I49, K54, G82
K45, P46, D31, I49, T50, G51
Epi#16
Q116, P114, Y108, M12, S39, S40, A23, A24, D8
Q116, P114, Y108, M12, Q37, S40, A23, A24, D8
R86, P114, Y108, M12, S39, S40, A23, A24, D8
Epi#22
D126, L133, D130, Y127, E122
D130, L124, D126, Y127, E122
D130, L128, D126, Y127, E122
Epi#23
R123, N118, E122, L124, L11, A23
R123, N118, E122, L124, L11, A36
R123, N118, E122, L124, L11, A24
Epi#24
E109, G90, E110, P114, R86, E80, Q78
E57, K54, E58, P59, F56, A81, Q78
E58, G60, E57, P59, F56, E80, Q78
Epi#25
R86, K88, I107, E109, E110
R86, K88, I77, E80, V84
R86, K88, I107, E109, V112
Epi#27
57, E58, D55, K54
D55, E57, E58, K54
Epi#28
V34, D31, Q101, K98, E122, L128, Q131, G132, D130
I129, D126, Q131, L128, E122, K98, Q101, G100, D130
I72, H68, Q47, F44, E48, K45, Q43, G70, K73
I72, H68, Q47, I49, E48, K45, Q43, G71, K73
Epi#29
I129, Q101, L128, R123, E122
G132, Q131, L128, R123, E122
Epi#30
I77, M75, A63, H61, P59, L62, P64
G90, M75, A63, H61, K54, L62, P64
Epi#33
Q116, Y108, P111, S91, K89
K88, Y108, P111, S91, K8
Epi#34
V76, P64, M75, L62, G51, T50, P46
I27, W35, S33, V34, G32, T50, P46
V76, P64, T65, L62, G51, T50, P46
Epi#35
A24, L22, A23, S39, M12, 1107
A23, L11, A36, S39, M12, I10
Epi#37
Y127, R123, L124, K97, N118
Y108, A23, L11, R123, Y127
A23, A24, L11, R123, Y127
Epi#39
A81, E57, H61, T65, P64, G60, L62
A81, E58, H61, T65, P64, G60, L62
Epi#40
R123, L11, A23, Y108, P111, S91
R123, L11, A24, Y108, P111, T113
Epi#41
P111, Y108, L22, V112, S91
P114, Y108, L22, V112, S91
Epi#43
I27, W35, A36, L11, Q37, S39, M12, 1107, T95
Epi#44
I77, R86, P114, Y108, S91, V112, P111
V120, Q116, P114, Y108, S91, V112, P111
L22, Q116, P114, Y108, S91, V112, T113
L22, Q116, P114, Y108, A23, V112, P111
Epi#47
I129, Y127, E122, M119, R123, L124, N118, R86, P114
L133, Y127, E122, M119, R123, L124, N118, R86, P114
Epi#48
E122, Q116, P114, P111, V112
S91, K88, P114, P111, V112
Epi#50
H10, Y6, W3, S2, T5
H10, Y6, W3, T5, S39
Epi#51
K73, H68, K45, Q47, P46, S33, W35
Q101, H30, D31, T50, K45, Q47, H68
Rag Weed Pollen5:
Epi#03
L4, K37, A33, I34, Y17
L4, K37, A33, I34, Y29
Epi#05
A33, N36, T40, G3, S20, L4
A33, N38, T40, G3, S20, Y25
A33, N36, G3, T40, S20, I22
Epi#06
A33, N36, D2, C19, K24, P21
A33, N38, D2, S20, K24, P21
Epi#09
I22, L4, D2, N38, D1, K37, A33, N36, T40
T9, G15, E7, V14, D30, K32, N36, T40, L4
T9, G15, E7, V14, D30, K32, N38, N36, L4
Epi#12
Y17, E7
Y6, E7
Epi#20
V27, K24, P21, L4, K37, D2, G3, D1
V27, K24, P21, L4, N36, D2, G3, D1
Epi#22
D1, D2, L4, K37
D1, D2, P21, K24
D2, L4, T40, D1
Epi#23
N10, E7, Y6, L4, P21
Epi#25
K32, I34, D30, V14
K37, I34, D30, V14
K16, I34, D30, V14
Epi#33
K32, Y17, V27, S20, K24
K16, Y6, P21, S20, K24
Epi#34
I22, P21, S20, V27, G12, Y17, T9
I22, P21, S20, V27, G12, Y29, S31
Epi#40
G12, G15, Y29, K37, T40
G15, G12, Y17, K16, T9
G12, G15, Y29, K32, S31
Epi#41
P21, Y6, L4, S20
Epi#44
L4, D2, P21, Y25, S20, V27, T40
L4, D2, P21, Y25, S20, G3, T40
Vesv5:
Epi#01
L59, P67, P65, K143, K144, N64, Y140, R62, T61
L59, P67, P70, R57, K204, N73, Y201, Q202, T203
L59, P67, P69, R57, K72, N73, Y201, Q202, T203
L152, N149, P142, K145, K143, N64, Y140, R62, T61
Epi#02
L9, K7, Q108, P191, Y107, R102, V13
L9, K7, Q108, S192, Y107, R102, V13
Epi#03
L9, K7, A105, I6, Y3
Epi#04
K106, Y107, S192, Q108, K7, A105, I6
K106, Y107, S192, Q108, K7, V13, G12
Epi#05
G58, A56, R57, P69, G66, R62, T61, L59
G58, A56, R57, P69, G63, R62, T61, L59
Epi#06
G66, N64, D139, R62, K138, P67
G66, N64, D139, R62, K138, P65
G63, N64, D139, R62, K138, P67
Epi#08
K145, E199, S147, F151
K196, E198, S147, F151
K144, E199, S147, F151
Epi#09
L152, D150, S147, K144, N64, T61, L59
L152, D150, D139, K153, F151, S147, N197
D139, N64, R62, D135, K153, F151, S147, N197
Epi#10
E199, N197, N194, S147, F151, G148, K143
E199, N197, N194, S147, F151, G148, K196
E199, N197, N194, S147, F151, G148, K145
Epi#11
K179, I176, Q177, V30, E178
K29, I176, Q177, V30, E178
Epi#12
Y201, E199
Epi#13
S147, L200, P142, T203, A56, P70, L59, P67, G66
S147, L200, P142, T203, A56, P69, L59, P67, G58
S147, L200, P142, T203, A56, P70, L59, P67, G63
S147, L200, P142, T203, A56, P69, L59, P67, Y140
Epi#15
K106, P191, D103, I6, K5, A105
K106, P191, D103, I6, K7, G12
Epi#16
R57, P70, Y201, M74, Q53, N76, D50, A56, N73
R57, P69, Y201, M74, Q53, N76, D50, A56, N73
Q108, P191, Y107, R102, Q111, S192, D103, A105, N2
Epi#18
R57, L59, T61, P67, N64
R57, L59, T61, P65, N64
Epi#19
E167, N164, S192, Q108, R102, K7
E198, N194, S192, Q108, R102, K7
D103, T100, C8, Q108, R102, K7
Epi#22
L9, D103, T100, K10
A105, D103, L9, K7
D50, L45, D43, T37, K38
S147, D150, L152, K153
Epi#23
K196, N197, E199, N164, Q202, P70
K145, N197, E199, N164, Q202, P69
Epi#24
E198, K196, E199, P142, T203, P69, K143
E198, K145, E199, P142, T203, P70, K204
E198, K196, E199, P142, T203, P70, K72
E198, K145, E199, P142, F146, F151, K196
Epi#25
R57, K54, D50, N76
R57, K54, D50, E47
Epi#27
D43, E40, D125, K122
D50, E47, D43, K38
Epi#28
Q202, E199, K196, F151, S147, K144
Q202, E199, K196, F195, S147, K145
Epi#29
G58, R57, L59, R62, E136
G148, K145, L200, F195, E199
G148, K145, L200, F195, E198
Epi#33
K23, Y19, P24, S21, A16, K18
K23, Y34, P24, S21, A16, R102
Epi#34
I176, W180, T116, L115, G117, T119, S118
V31, P24, S21, L22, G35, Y34, T37
Epi#37
P69, R57, L59, K54, D50
P70, R57, L59, R62, D135
A56, R57, L59, R62, N64
P69, R57, L59, R62, D139
Epi#39
E199, L200, T203, P70, G58, L59
E198, L200, T203, P69, G58, L59
Epi#40
R57, L59, G58, T203, P69, T61
R57, L59, A56, Y201, K204, T203
R57, L59, A56, Y201, K72, T203
Epi#41
P24, Y19, L22, S21
P24, Y34, L36, S33
Epi#42
P191, S192, Q111, H98, R102, Q108
Epi#44
L59, R57, P70, Y201, A56, G58, T61
L59, R57, P69, Y201, A56, G58, T203
L59, R57, P70, Y201, A56, G58, P67
Epi#45
K153, H156, F151, Y140, N149, D150, L152
D135, H156, F151, Y140, N141, D150, L152
K143, P142, F146, Y140, N149, D150, L152
Epi#47
G58, L59, R57, M74, A56, Q202, N73, P70, P69
G148, Y140, R62, L59, R57, A56, N73, P70, P67
G66, G63, R62, L59, R57, A56, N73, P70, P67
G155, E136, R62, L59, R57, A56, N73, P70, P67
Epi#48
Q202, K204, P69, P67, G58
Q202, K204, P70, P67, G63
Q202, K72, P70, P67, G66
Epi#49
D125, D43, L45, V78, Q42, Q39, T37, K38
D125, D43, L45, V78, Q42, Q39, T37, K41
Epi#50
H98, Y96, W90, L22, S21
H98, Y96, W90, P24, S33
Epi#52
F0, A16, R102, W90, N25, Q95
F0, A16, R102, W90, N25, Q93
Betv1:
Epi#03
SAS: 270, Size 11.07: L24, K20, H76, I23, Y81
SAS: 204, Size 11.96: L24, K20, A16, I23, Y81
Epi#05
SAS: 298, Size 14.01: G110, A106, A16, P14, G111, T10
SAS: 242, Size 14.01: G110, A106, A16, P14, G111, T107
Epi#08
SAS: 464, Size 11.12: K123, E127, G1, H121, F3
SAS: 455, Size 12.95: K129, E127, G1, H121, F3
SAS: 438, Size 13.31: K123, D125, G1, H121, F3
SAS: 428, Size 11.12: K123, E127, V2, H121, F3
SAS: 425, Size 11.65: K123, E127, G124, H121, F3
Epi#09
SAS: 466, Size 20.55: D109, A106, V105, K80, A16, T77
SAS: 444, Size 20.55: D109, G110, V105, K80, A16, T77
SAS: 427, Size 20.55: D109, G111, V105, K80, A16, T77
SAS: 398, Size 19.17: T10, G110, V105, K80, A16, T77
SAS: 381, Size 19.17: T10, G111, V105, K80, A16, T77
Epi#10
SAS: 558, Size 15.18: D75, T77, N78, A106, F79, R17, K20
SAS: 549, Size 21.96: E6, T7, N4, F3, G1, K123
SAS: 517, Size 13.31: D75, T77, N78, A16, F79, R17, K20
SAS: 497, Size 15.13: D75, T77, N78, A16, F22, R17, K20
Epi#12
SAS: 335, Size 9.08: T7, Y5, E6, N4
SAS: 331, Size 11.28: R145, Y150, E148, L152
SAS: 326, Size 10.37: R70, Y83, E73, P50
SAS: 311, Size 10.32: 1116, Y5, E6, N4
SAS: 308, Size 8.33: R145, Y150, E148, S149
Epi#18
SAS: 328, Size 24.67: S117, K103, F79, V105, A16, Y158, L24
Epi#22
SAS: 533, Size 9.96: D125, D93, K123, E127
SAS: 533, Size 9.96: D93, D125, K123, E127
SAS: 476, Size 11.40: D125, D93, K123, E96
SAS: 476, Size 11.40: D93, D125, K123, E96
SAS: 400, Size 17.99: D125, D93, P90, E87
Epi#23
SAS: 451, Size 22.02: K68, N43, E42, S57, F64, P63
SAS: 450, Size 22.02: K55, N43, E42, S57, F64, P63
SAS: 428, Size 22.02: K68, N43, E42, S57, L62, P63
SAS: 427, Size 22.02: K55, N43, E42, S57, L62, P63
SAS: 412, Size 18.85: K68, N43, E42, S40, F30, P35
Epi#24
SAS: 734, Size 18.92: E127, K123, E96, P90, S136, E131, K129
SAS: 729, Size 18.92: D93, K123, E96, P90, S136, E131, K129
SAS: 716, Size 19.57: E127, K123, E96, P90, S136, E131, K134
SAS: 711, Size 19.57: D93, K123, E96, P90, S136, E131, K134
SAS: 708, Size 20.49: D125, K123, E96, P90, S136, E131, K129
Epi#25
SAS: 467, Size 12.68: R70, K55, I44, E42, E45
SAS: 425, Size 12.68: R70, K54, I44, E42, E45
SAS: 420, Size 14.01: R70, K55, I44, D27, E42
Epi#27
SAS: 613, Size 14.25: D93, E127, A130, E131, K129
SAS: 595, Size 16.54: D93, E127, A130, E131, K134
SAS: 592, Size 16.70: D125, E127, A130, E131, K129
SAS: 574, Size 19.79: D125, E127, A130, E131, K134
SAS: 524, Size 18.78: D93, E127, A130, E131, K137
Epi#28
SAS: 869, Size 21.93: V33, Q36, F58, E60, L62, F64, P63, K65
SAS: 837, Size 21.83: V33, Q36, F58, E60, L62, F64, G61, K65
SAS: 808, Size 24.56: V33, Q36, F58, E60, L62, F64, P90, K65
SAS: 783, Size 21.83: V33, Q36, F58, E60, K65, F64, S57, K68
SAS: 782, Size 21.83: V33, Q36, F58, E60, L62, F64, S57, K65
Epi#29
SAS: 516, Size 9.52: G61, K65, L62, E60
SAS: 440, Size 8.70: G61, P63, L62, E60
SAS: 371, Size 6.78: G61, P59, L62, E60
Epi#32
SAS: 374, Size 17.88: F79, A16, A106, D109, V12
SAS: 354, Size 20.42: F22, A16, A106, D109, V12
Epi#33
SAS: 541, Size 18.79: K65, F64, P90, S136, A135, K134
SAS: 498, Size 9.15: Q36, F30, P35, S39, K32
SAS: 496, Size 11.27: Q36, F30, P35, S40, K32
SAS: 494, Size 12.19: Q36, F58, P35, S39, K32
SAS: 493, Size 18.79: K65, Y66, P90, S136, A135, K134
Epi#36
SAS: 447, Size 19.17: T77, A16, A106, V12, G110, T10
SAS: 430, Size 19.17: T77, A16, A106, V12, G111, T10
SAS: 392, Size 19.17: T77, A16, A106, V105, G110, T10
SAS: 391, Size 19.17: T77, A16, A106, V12, G110, T107
SAS: 375, Size 19.17: T77, A16, A106, V105, G111, T10
Epi#40
SAS: 246, Size 21.55: A106, A16, Y158, S155
SAS: 223, Size 13.25: A135, A130, Y5, T7
SAS: 196, Size 14.88: A135, A130, Y5, S117
SAS: 178, Size 10.62: A135, G140, T142, S136
Epi#44
SAS: 530, Size 19.04: L24, R17, D156, Y150, S149, V12, T10
SAS: 492, Size 19.04: 123, R17, D156, Y150, S149, V12, T10
SAS: 490, Size 17.39: L24, R17, D156, Y150, S149, V12, P14
SAS: 483, Size 23.09: L24, R17, D156, Y158, A16, A106, P108
SAS: 474, Size 20.83: L24, R17, D156, Y150, S149, V12, T107
Epi#45
SAS: 606, Size 21.41: K32, P35, F30, Y150, R145, V12
SAS: 546, Size 20.89: K32, P31, F30, Y150, R145, V12
SAS: 533, Size 15.19: K32, P35, F30, Y150, R145, G140
SAS: 533, Size 12.63: K32, P35, F30, Y150, R145, V33
SAS: 532, Size 19.60: K32, P35, F30, N28, D27, I44
Epi#47
SAS: 333, Size 21.03: R17, L24, N28, P31, P35
SAS: 300, Size 22.72: R17, L24, N28, P31, S39
SAS: 298, Size 21.80: R17, L24, N28, P31, S40
SAS: 269, Size 24.87: R17, L24, N28, P31, S57
Epi#48
SAS: 436, Size 14.26: S57, K65, P90, P63, G61
SAS: 414, Size 17.96: S39, K32, P35, P59, G61
SAS: 412, Size 17.96: S40, K32, P35, P59, G61
SAS: 389, Size 18.32: S57, K65, P63, P90, G92
SAS: 365, Size 21.15: S57, K65, P59, P35, V33
[0578] "SAS" is solvent accessible surface. "Size" is the total
surface area of the epitope in A2.
Derf2:
Epi#02
A98, K100, S101, P99, R128, R31
A98, K100, R128, P99, R31, V94
T91, N93, P95, P34, R31, R128
L61, N93, P95, P34, R31, R128
Epi#03
L40, K15, A39, I13, Y86
L40, K14, A39, I88, Y90
Epi#05
G32, A98, R31, P34, G20, T36, T91, Y90
G32, A98, R31, P34, G20, T36, T91, V94
G32, A98, R31, P34, G20, T36, T91, L37
G32, A98, R31, P34, G20, T36, T91, V18
Epi#06
A98, P99, D129, R31, K96, P95
G32, P99, D129, R128, R31, P95
A98, P99, D129, R31, K33, P95
A98, P99, D129, R31, K96, P34
A98, P99, D129, R128, K126, P26
Epi#07
T107, S57, D59, S101, R128, A98, P99, D129
T107, S57, D59, S101, R31, A98, P99, D129
Epi#08
K15, D87, V76, H74, F75
K14, D87, V76, H74, F75
K77, D87, V76, H74, F75
Epi#09
L61, D64, I68, H74, F75, T70, N71
N114, N46, D113, K48, N71, T70, T49
G83, N46, D113, K48, N71, T70, T49
Epi#10
L40, I13, D42, N44, V81, K48, N46, N114, G115
L40, I13, D42, N44, V81, K82, N46, N114, G115
L37, D19, G20, V18, V3, D4, K6, A120, T107, V105
Epi#11
F75, K51, I111, Q45, V116, D113
F75, K51, I111, Q45, V81, D113
Epi#12
Y90, E38
Epi#13
H30, R31, P95, A98, P99, S101, G60, L61
Epi#15
K96, P99, D129, 128, R128, A98
K96, P99, D129, I127, R128, A98
[0579] K96, P99, D129, 129, R128, A98
K55, P66, D64, I68, T70, G67
Epi#18
R31, R128, I28, G125, T123, H124, V105
R31, R128, I127, G125, T123, H124, V105
Epi#22
D1, M17, D4, V3, K6
D1, M17, D19, P34, K96
D1, M17, D4, V5, K6
Epi#23
K14, N11, E12, N44, Q85, P79
K14, N11, E12, N10, Q45, P79
K14, N11, E12, N44, Q84, P79
K14, N11, E12, L40, Q85, P79
Epi#24
D129, K100, E102, P99, R128, R31, K96
E62, G60, E102, P99, R128, R31, K96
D129, K126, E102, P99, R128, R31, K33
D129, K126, E102, P99, R31, P95, K96
Epi#25
R31, K96, I97, D59, E62
R128, R31, 197, D59, E102
R128, K126, I127, E102, N103
Epi#27
D64, E62, D59, K100
D59, E62, D64, K55
D87, E38, D19, K33
D19, E38, D87, K15
D19, E38, D87, K14
D19, E38, D87, K77
Epi#28
V16, D87, Q85, K14, E12, K15, Q2, D1
I13, D87, Q85, K14, E12, K15, Q2, D1
V3, D1, Q2, K15, E12, K14, Q85, D87
L40, D87, Q85, K14, E12, K15, Q2, D1
I88, D87, Q85, K14, E12, K15, Q2, D1
V76, D87, Q85, K14, E12, K15, Q2,D1
V18, D1, Q2, K15, E12, K14, Q85, D87
Epi#29
G32, N93, L61, E62
V94, N93, L61, E62
Epi#30
G60, I97, A98, H30, K96, P34, P95
I68, N71, H74, K77, P79, V81
G32, I97, A98, H30, K96, P95, P34
Epi#34
V105, P26, S24, G125, R128, S101, P99
W92, P34, T91, V94, R31, S101, P99
I28, P26, T123, G125, R128, S101, P99
Epi#37
A120, V16, L40, K14, N11
A39, V16, L40, K14, N11
Y90, A39, L40, K14, N11
Y86, A39, L40, K14, N11
Epi#39
A120, E38, T91, P34, G20, L37
A39, E38, T91, P34, G20, L37
Epi#40
G20, L37, A120, T123, K6, S24
A39, L37, A120, T123, K6, S24
G20, L37, A120, T107, K6, T123
Epi#41
P34, L37, V106, S57
Epi#42
P26, S24, G125, R128, R31
P99, S101, G125, R128, R31
Epi#44
V16, Q2, D19, P34, W92, Y90, A39, V18, T91
V16, Q2, D19, P34, W92, Y90, A39, V5, T123
V3, Q2, D19, P34, W92, Y90, A39, V18, T91
Epi#45
K77, H74, F75, N71, D69, G67
K77, H74, F75, N71, D69, V76
K77, H74, F75, N71, D69, V65
Epi#46
A98, R128, R31, P95, N93, G32
A98, R128, R31, P34, G20, Q2
Epi#48
Q2, D19, P34, P95, G32
H30, K96, P95, P34, G20
Epi#49
D87, D42, L40, Q85, Q84, C78, T47, Q45, K48
D87, D42, L40, Q85, Q84, C78, T47, Q45, K82
Epi#50
D19, W92, P34, T91
D19, W92, P34, P95
D19, W92, T91, T36
Epi#51
D129, H30, K33, R31, R128, K126, H124
R31, H30, D129, R128, K100, K126, H124
T123, H124, K126, R128, R31, K33, H30
Derp2:
Epi#03
L17, K89, A39, I13, Y86
L17, K89, A72, I88, Y90
L17, K89, A72, I52, Y90
Epi#04
K15, S1, Q2, K14, V16, L17
K15, S1, Q2, K14, A39, L17
K15, S1, Q2, K14, V40, I13
Epi#05
G60, A56, L61, P99, G32, R31, H30, I97
G60, A56, L61, P99, G32, R31, H30, I28
Epi#06
G60, A56, D64, S57, K55, P66
G83, N46, D114, T49, K48, P79
G60, N103, D59, S101, R31, P95
Epi#08
K55, D64, S57, V106, F35
K55, E62, S57, V106, F35
Epi#09
L61, G60, E102, R128, 128, K126, N103, T123, V105
L61, G60, E102, R128, 1127, K100, N103, T123, V105
L61, G60, E102, R128, 1127, H124, N103, T123, V105
Epi#10
SAS: 435, Size 24.47: D69, T91, N93, F35, G32, R31
SAS: 422, Size 20.74: E38, T91, N93, F35, G32, K96
Epi#11
K14, I13, Q85, V81, E42
K15, I13, Q85, V81, E42
K14, I13, Q85, V40, D87
Epi#12
Y86, E42
Y90, E53
Y90, E38
Epi#13
H30, A125, P26, T123, A122, P19, L37, P34, W92
H30, A125, P26, T123, A122, H124, S24, G23, G20
H30, A125, P26, T123, A122, P19, L17, G20, F35
Epi#15
K55, P66, D69, I68, K89, A72
K55, P66, D69, I68, K89, A39
K55, P66, D64, I54, K109, G115
K55, P66, D64, I54, K109, A9
Epi#18
R31, 129, A125, S101, E102, N103
R31, 129, A125, S101, E102, V104
R31, 129, A125, T123, A122, V105
Epi#22
D69, P66, D64, V65, K55
D64, P66, D69, T91, K89
D59, L61, D64, P66, W92
D59, L61, D64, V65, E62
D69, P66, D64, V65, E53
Epi#24
D64, K55, E62, P99, R31, P34, K96
E53, K55, E62, P99, R31, P95, K96
D64, K55, E62, P99, R31, A98, K96
Epi#25
R31, H30, I28, E102, N103
R128, K126, I127, E102, N103
R128, K126, I28, E102, V105
Epi#27
D64, E53, D69, K89
D69, E53, D64, K55
D59, E62, D64, K55
Epi#28
V40, D87, Q85, E42, Q84, G83, K82
G20, H22, Q2, L17, E38, L37, Q36, P34, K33
G20, H22, Q2, L17, E38, L37, F35, P34, K33
Epi#29
I97, K100, L61, E62
G60, N103, L61, E62
I127, N103, L61, E62
Epi#30
G60, N103, S101, H30, K96, I97, P95
G60, N103, A125, H30, K96, I97, P95
I28, I127, A125, H30, K96, I97, P95
Epi#33
Q36, F35, V106, S57, A56, K55
K33, F35, V106, S57, A56, K55
Epi#34
I28, P26, S24, G23, G20, T123, S57
I28, P26, S24, V3, G20, T123, T107
W92, P34, T91, V18, G20, T123, P26
Epi#37
P66, V63, L61, K100, N103
P95, A98, L61, K100, N103
P19, V18, L17, K89, D87
P19, V3, L17, K89, D87
T123, V104, L61, K100, N103
Epi#38
L61, G60, E102, A125, V105, N103, P99, S57
L61, G60, E62, A56, V105, N103, P99, S57
Epi#39
A125, E102, H124, T123, P26, G20, L17
Epi#40
G60, L61, A56, T107, K6, T123
A39, L17, G20, T123, P26, S24
G60, L61, A56, T107, K55, S57
G60, L61, A56, T123, K126, S101
Epi#41
P19, L17, V3, S1
P19, L17, V5, S24
Epi#44
V65, D64, P66, W92, Y90, A39, V18, P19
L61, D64, P66, W92, Y90, A39, V18, T91
Epi#45
R31, P34, F35, N93, V94
K96, P34, F35, N93, G32
Epi#47
I127, S101, R31, 197, A98, L61, N103, P99, P95
I28, S101, R31, 197, A98, L61, N103, P99, S57
Epi#48
H30, K96, P95, P99, G60
H30, K96, P34, P19, G20
H30, K96, P34, P19, V18
H30, K96, P34, P95, V94
H30, K96, P34, P19, V3
E38, K89, P70, P66, V65
H30, K96, P95, P34, G32
Q36, K89, P70, P66, V65
Epi#50
D69, Y90, W92, P66, P70
D69, Y90, W92, P34, P95
D69, Y90, W92, T91, P34
D69, Y90, W92, V94, P95
D69, Y90, W92, L37, P19
Epi#51
K126, H124, E102, R128, 128, R31, H30
T123, H124, K126, R128, 128, R31, H30
D4, H124, K126, R128, 128, R31, H30
[0580] PhIp2:
Epi#02
T87, K85, Q61, S38, R34, R67
T87, K85, Q61, P63, R34, V42
Epi#03
K10, A90, I88, Y86
K10, A18, I88, Y86
Epi#04
R34, S38, Q61, K85, T87, I88
R34, S38, Q61, K85, T87, A90
Epi#05
G47, A18, S12, T87, G89, T91, T5, V1
G73, A29, L69, T27, G50, T53, T45, V42
G11, A18, L20, T91, G89, A90, T87, I88
Epi#06
A93, P94, D79, R34, Q61, P59
A93, P94, D79, R34, Q61, P83
A93, P94, D80, R34, Q61, P59
A93, P94, D79, R34, Q61, P63
Epi#08
K10, E9, G11, A18, H16, F54
K46, E48, G47, A18, H16, F54
K10, E9, S12, A18, H16, F54
Epi#09
L69, T27, G73, N76, R67, V77, D79, R34, A43, T45, V42
L69, T27, A29, E30, R67, V77, D80, R34, A43, T45, V42
Epi#10
D55, A18, N13, S12, F54, G47, K46
T45, A18, N13, S56, F54, G47, K46
Epi#09
L60, S56, E57, D55, K15, N13, S12, G11
L60, S56, E57, D55, H16, F54, T45, T53
L60, S56, E57, D55, H16, F54, T45, G47
Epi#12
Y86, E84
Y23, E24
Epi#18
N76, R67, F78, V81, A93, Y92, T91, T5, P2, V1
Epi#19
D39, W41, S38, Q61, R34, G37
E40, W41, S38, Q61, R34, A43
Epi#22
D79, P94, D80, P83, K85
D79, P94, D80, P63, K85
Epi#23
K10, N13, E14, L60, Q61, P59
K10, N13, E14, L60, Q61, P83
K10, N13, E14, L60, Q61, P63
Epi#24
E58, K15, E57, P59, S56, E14, Q61
D55, K15, E57, P59, S56, E58, Q61
Epi#25
R34, R67, W41, D39, E40
Epi#26
S38, E40, W41, V42, E32, E30
S38, E40, W41, V42, A43, E32
Epi#27
E14, E57, E58, K15
D55, E14, E84, K85
Epi#28
G37, H36, Q61, K85, E84, L60, F54, A43, K46
G37, H36, Q61, K85, E84, L60, F54, S12, D55
G37, H36, Q61, K85, E84, L60, F54, S56, D55
G37, H36, Q61, K85, E84, L60, F54, A43, R67
G37, H36, Q61, K15, E57, L60, F54, A43, K46
G37, H36, Q61, K85, E84, L60, F54, S12, K15
G37, H36, Q61, K85, E84, L60, F54, S56, K15
G37, H36, Q61, K85, E84, L60, F54, A43, R34
G37, H36, Q61, K85, E84, L60, F54, A18, D55
Epi#29
G73, K72, L69, R67, E30
I88, N13, L60, F54, E57
G25, K72, L69, R67, E32
V77, K75, L69, R67, E30
G37, H36, L60, F54, E57
G37, Q61, L60, F54, E57
Epi#30
I88, N13, S12, H16, K15, P59, L60
I88, N13, S56, H16, K15, L60, P59
I88, N13, A18, H16, K15, P59, L60
Epi#33
K46, F54, V42, S56, K15
H16, F54, V42, S56, K15
Epi#34
V1, P2, T5, V4, P94, Y92, T87
V1, P2, T5, L20, G89, T91, T87
V81, P94, T5, V1, P2, Y92, T91
Epi#37
T27, A29, L69, K72, D26
A43, R67, L69, K75, N76
Epi#38
L20, G89, E9, A18, N13, P59, S56
Epi#40
G49, L20, G89, Y86, K85, T87
G49, L20, G89, T87, K10, S12
G49, L20, G89, T87, K10, T7
Epi#44
V77, R67, D79, P94, Y92, A93, V1, P2
L69, R67, D79, P94, Y92, A93, V1, T5
Epi#45
D79, P94, F78, N76, M74, L69
D80, P94, F78, R67, D79, V77
K3, P94, F78, N76, M74, G73
Epi#46
A43, R67, R34, P63, H36, Q61
V77, R67, R34, P63, H36, G37
L69, R67, R34, P63, G37, Q61
Epi#47
G37, E35, E40, A43, R34, L60, N13, P59, S56
V77, E32, E40, A43, R34, L60, N13, P59, S56
S38, G37, E40, A43, R34, L60, N13, P59, S56
Epi#48
E24, K3, P94, P2, V1
E84, D80, P94, P2, V1
Epi#50
D39, W41, A43, T45
D39, W41, V42, T45
Epi#51
D79, H36, E84, T87, K10, G11, H16
D39, H36, Q61, K85, P63, R34, W41
D79, H36, E40, D39, G37, R34, W41
Q61, H36, E84, T87, K10, G11, H16
Example 11
[0581] For this example a third-generation epitope sequences were
determined for some additional enzymes and redetermined for all of
the enzymes in example 1-3. New enzymes are AMG (AMG pdb), BPN''
(1sup.pdb), Esperase (structure see Appendix D), Natalase
(structure modelling based on SP722), Amylase-AA560 (Structure
modelling based on SP722), Protease A, Alcalase, Protease B,
ProteaseC, ProteaseD, ProteaseE, Properase and Relase based on
their sequences and structures. The structures of Protease B,
Properase, Relase, Protease A, Alcalase, ProteaseC, ProteaseD and
ProteaseE can be found by "Homology modelling" (see above) and
computer modelling of the epiope patterns that had been assembled
in our database (shown in Table 8). Furthermore, the epitope
sequences were redetermined for CAREZYME, Laccase, PD498, Savinase,
Amylase SP722, and Cellulase, according to the method.
[0582] The protein surface is scanned for epitope patterns matching
the given "consensus" sequence of about 6-12 residues. First,
residues on the protein surface that match the first residue of the
consensus sequence are identified. Within a specified distance from
each of these, residues on the protein surface that match the next
residue of the consensus sequence are identified. This procedure is
repeated for the remaining residues of the consensus sequence. The
method is further described under the paragraph "Methods" above and
the program can be found in Appendixes.
[0583] The critical parameters used in this screening included:
[0584] i) a maximal distance between the alpha-carbon atoms of
subsequent amino acids, [0585] ii) a minimal accessability of the
amino acid of 20 .ANG.2, [0586] iii) the largest maximal distance
between the most distinct amino acids should be less than 25 .ANG.
[0587] iv) the best epitope were taken, [0588] v) the homology with
the epitope pattern of interest was 100%
[0589] In this way a number of potential epitopes are identified.
The epitopes are sorted according to total surface accessible area,
and certain entries removed: [0590] 1) Epitopes that contain the
same protein surface residue more than once. These are artefacts
generated by the described algorithm. [0591] 2) Epitopes which are
"too big", i.e. where a distance between any two residues in the
epitope exceeds a given threshold.
[0592] The subtilisin sequences and positions mentioned in the
following are not given in the BPN' numeration but in the
subtilisins own numeration (see the alignment as described above in
Tables 1A and 1B).
[0593] The epitope sequences found were:
AMG:
Epi#01
L104, P123, P107, R125, R122, N182, S184, Q172, T173
L104, P107, P123, R125, R122, N182, S184, Q172, S453
L104, P107, P123, R125, R122, N182, S184, Q172, T452
Epi#02
L234, R241, S240, F237, T173, Y175, R122, R125
L234, R241, S240, F237, T173, Y169, R125, R122
L234, R241, S240, F237, T173, Y175, R125, R54
Epi#03
L291, K404, I288, Y289
L66, K61, H254, I253, Y329
Epi#04
R122, Y175, S184, Q172, Y169, A454, I455
R122, Y175, S184, Q172, Y169, N171, A451
R125, Y175, S184, Q172, Y169, T452, A451
Epi#06
G31, A24, D25, S30, A27, P41
G146, N145, D144, T148, S149, P467
A471, N145, D144, T148, S149, P467
Epi#07
G294, T290, S405, D293, S287, R286, P307, D283
G294, T290, S287, D293, S296, R286, P307, D283
G207, T204, S200, D214, S209, R160, P157, D153
G294, T290, S405, D293, S287, R286, P307, D309
Epi#08
A27, D25, S30, V111, F49
A24, D25, S30, V111, F49
Epi#09
S149, T148, G146, N145, A471, R68, N69, T72, V470
S73, S76, T72, N69, R68, A471, N145, T148
Epi#10
D238, N182, N236, S240, F237, R241, K244
D238, T173, N182, S239, F237, R241, K244
Epi#11
F49, F109,191, Q85, E113
Epi#12
Y363, E342
Y311, E308
Y175, E180
Epi#13
S119, W120, P123, A102, P94, S92, G90, L98
S119, W120, P123, A102, P94, S92, G96, G90
Epi#15
K244, P307, D283, I288, T290, G294
R160, P157, D153, I154, T462, G90
R286, P307, D283, I288, T290, G294
Epi#16
L410, P46, Y48, R413, S397, S394, A392, A393, N395
R160, P157, Y458, G456, S211, S209, A205, A201, D214
Epi#17
A201, S209, R160, S459
A205, S209, R160, S459
Epi#19
D44, N45, S411, Q409, R413, L410
D47, N45, S411, Q409, R413, L410
Epi#20
K61, P434, L66, L423, N427, D65, G70, D71
Epi#22
D357, S356, D349, V346, D345
D349, S356, D357, A359, D345
D357, S356, D349, L348, D345
Epi#23
K404, N292, E299, S298, L295, A300
K404, N292, E299, S296, L295, A300
Epi#24
D336, K337, E259, P258, S431, L332, K378
D336, K337, E259, P258, S431, R429, K378
D336, K337, A261, P258, S436, E259, Q338
Epi#25
R125, R122, W120, E180, N182
R241, K244, E308, N313
Epi#26
W212, S200, E198, W437, V197, G438, E259
W212, S200, E198, W437, V197, A201, D214
Epi#27
D283, E280, D349, K352
D403, E408, D406, K404
D349, E280, D283, K244
D349, E280, D283, K279
Epi#28
L332, D336, Q338, K337, E259, C262, P272, D345
V374, D336, Q338, K337, E259, C262, P272, D345
G339, D336, Q338, K337, E259, C262, P272, D345
Epi#29
L295, G294, L291, R286, E299
I288, K404, L291, R286, E299
L348, K352, L354, F380, E299
Epi#33
K352, Y355, V374, S371, S365, K337
K352, Y355, V374, S365, S340, K337
Epi#34
V463, W466, S468, V470, P467, T464, T462
I469, W466, S468, V470, P467, T464, T462
I154, W466, S468, V470, P467, T464, T462
V463, W466, S468, V470, P467, S465, T464
Epi#37
T362, A359, L348, K352, D357
T360, V346, L348, K352, D357
T362, A359, L348, K352, D349
Epi#38
G438, E259, A435, R68, L66, N69, P434, S431
Epi#39
A353, E299, R286, P307, G243, L234
A300, E299, R286, P307, G243, L234
Epi#40
A205, L143, G146, Y147, P467, T464
G146, L143, A205, T204, A201, S209
A451, A450, T448, P446, S444
Epi#41
P467, Y147, L143, V206, S149
Epi#42
L66, P434, S431, N430, R429, R428
L104, P123, S95, G101, P94, R122, R125
L104, P107, S95, G96, P123, R125, Q172
Epi#44
L143, Q140, D144, W141, Y147, S468, V470, T72
V206, Q140, D144, W141, Y147, S468, V470, P467
S211, Q216, D214, P218, Y223, A451, A450, T448
S211, Q216, D214, P218, Y223, A450, G447, T448
Epi#45
R413, P46, F49, Y50, N110, D112, G31
R413, P41, F49, Y50, N110, D33, G31
D44, P46, F49, Y50, N110, D112, G31
Epi#46
Y175, R125, R122, P123, G174, Q172
Y169, R125, R122, P123, G174, Q172
V432, R429, R428, P434, N69, G70
Y175, R125, R122, P94, N93, G90
Y175, R122, R125, P123, N182, G121
Y175, R125, R122, P94, G101, A102
Y175, R125, R122, P94, G118, A115
Y175, R125, R122, P94, G101, G96
Y175, R122, R125, P123, N182, G183
Epi#48
S211, D214, P218, P446, G447
E259, K337, P258, P434, V432
S215, D214, P218, P446, G447
S209, D214, P218, P446, V445
E259, K337, P258, P434, V433
Epi#50
R122, Y175, W120, T117, S119
R125, Y175, W120, S119, T117
Epi#51
T390, H391, E408, Q409, R413, S411, W317
T390, H391, E408, S405, I288, K404, W317
D406, H391, E408, Q409, R413, S411, W317
T390, H391, E408, D406, K404, Q409, W317
Epi#52
W437, A260, T266, R273, W228, D264, Q225
BPN':
Epi#02
T255, K256, S260, F261, P194, Y262, R186, V203
L257, K256, S260, F261, P194, Y262, R186, V203
T253, K256, S260, F261, P194, Y262, R186, V203
Epi#03
K141, A137, I108, Y104
K136, A137, I108, Y104
K136, A134, I108, Y104
Epi#04
K265, Y262, S188, Q185, R186, N184, L257
K265, Y262, S188, Q185, Y263, R186, L257
K265, Y262, S188, Q185, R186, N184, G258
K265, Y262, S188, Q185, Y263, R186, G258
Epi#05
G80, A1, N77, P40, G211, S38, S37, V44
G80, A1, N77, P40, G211, S38, S37, L42
G127, A152, N155, T164, G160, S158, S188, Y262
Epi#06
G211, N212, D36, S37, K43, P40
G80, N212, D36, S38, K43, P40
G211, N212, D36, S38, K43, P86
Epi#08
K256, D259, S260, F261
K43, D36, S38, V44, F58
Epi#09
S105, S132, A133, A137, D140, K141, A144, S145, N118
S248, T244, A144, S145, D120, K27, N118, A116, N117
Epi#10
E54, T55, N57, S37, F58, G46, K43
T55, A48, N57, S37, F58, G46, K43
E54, T55, N57, S49, F58, G46, K43
Epi#11
K136, I108, Q103, V51, D98
Epi#12
Y171, E195
Epi#13
S101, W106, P52, T55, A48, P56, S49, G47, F58
S105, W106, P52, T55, A48, P56, S49, G47, W113
Epi#15
N25, P239, D120, I115, K141, A144
N240, P239, D120, I115, K141, A144
Epi#16
Q271, P14, Y21, G20, Q19, S18, A15, A272, N252
Q59, P210, Y214, G211, S38, D36, D61, A99, D98
Epi#17
A187, S188, R186, S183
A187, S188, R186, S182
Epi#18
N184, R186, S188, G157, S158, T159, S161
N184, R186, S188, G157, S158, T159, S162
N184, R186, S188, G157, S158, E156, N155
N184, R186, S188, G157, S158, E156, F189
Epi19
E156, N155, S188, Q185, R186, L257
E156, N155, S188, Q185, R186, G258
E156, N155, S188, Q185, R186, A187
Epi#22
D197, S260, D259, L257, K256
D197, S260, D259, Y263, K256
Epi#23
N155, E156, S188, Q185, A187
Epi#24
E156, G166, E195, P194, S260, L257, K256
D259, G264, E195, P194, S260, L257, K256
D197, K170, E195, P194, S260, L257, K256
Epi#25
K141, I115, D120, N25
K141, I115, D120, N118
K141, I115, E112, N118
Epi#26
W113, S49, W106, P52, E54, D98
W113, S49, W106, P52, E54, D60
W113, S49, W106, V51, E54, D98
Epi#28
A99, D61, Q59, F58, E54, L96, Q103, G102, D98
A99, D98, Q59, F58, E54, L96, Q103, G100, D61
A99, D61, Q59, F58, E54, L96, Q103, S101, D98
Epi#29
G102, Q103, L96, E54
G100, Q103, L96, E54
Epi#30
I79, N76, S87, H17, S18, P14, V4
I79, N76, S87, H17, Q19, P14, V4
Epi#31
L257, Q185, N184, R186, F189, V203, I205, D181
L267, Q10, N184, R186, F189, V203, I205, D181
Epi#33
K213, Y214, P210, S38, S37, K43
Q59, F58, V44, S38, S37, K43
Epi#34
W106, P52, M50, G47, P56, T55, S53
W106, P52, S49, G47, P56, T55, S53
I115, W113, M50, V51, P52, T55, S53
I108, W106, S105, V51, P52, T55, S53
Epi#35
A99, L96, S49, M50, 1108
A99, L96, S49, M50, 1107
Epi#36
A137, A134, A133, G131, Y104, S105, Q103, V51, A48, W113
A134, A137, A133, G131, Y104, S101, Q103, V51, A48, W113
Epi#37
Y262, R186, L257, K256, D259
Y263, R186, L257, K256, N252
Epi#39
E156, T164, P129, G127, L126
E156, T164, P129, G128, L126
E156, T164, P129, G154, L126
E156, T164, P129, G166, L126
Epi#40
R247, L250, A272, T255, K256, S260
R186, L257, G258, Y263, K256, S260
G264, L257, G258, T255, K256, S260
Epi#41
P194, Y262, L257, S260
P194, Y263, L257, S260
Epi#42
P194, S260, G258, R186, Q185
Epi#44
S182, Q185, D181, Y6, S9, V4, P14
S183, Q185, D181, Y6, S3, V4, P5
S248, R247, D197, P194, Y262, S260, G258, T255
S53, P52, W106, Y104, S105, V51, T55
Epi#45
K170, P194, F261, Y262, R186, D181, V203
D197, P194, F261, Y262, R186, D181, V203
Epi#46
S162, S158, E156, N155, A187, Q185, N184, R186, S188
S188, S158, E156, N155, A187, Q185, N184, R186, S183
S158, S188, E156, N155, A187, Q185, N184, R186, S182
S161, S158, E156, N155, A187, Q185, N184, R186, S183
G160, S158, E156, N155, A187, Q185, N184, R186, S188
Epi#48
S38, K43, P40, P210, G211
S37, K43, P86, P14, V4
S38, K43, P40, P210, G215
Epi#50
H238, W241, T242, P239
H238, W241, T244, T242
H238, W241, T242, T244
Epi#51
T242, H238, Q275, Q271, P14, S18, H17
Q245, H238, Q275, K237, P239, T242, W241
Q275, H238, Q245, T242, R247, T244, W241
Q245, H238, Q275, Q271, P14, Q19, H17
CAREZYME Core:
Epi#01
P61, P165, K164, R158, N154, Y168, R153, S151
P137, P49, K44, K13, N32, Y54, Q36, T39
P61, P165, K164, R158, N154, S152, R153, S151
Epi#02
L115, N118, S117, R4, T6, Y147, R146, V129
L115, N118, S5, R4, T6, Y147, R146, V129
Epi#03
K44, A43, I38, Y54
K13, A43, I38, Y54
Epi#04
R153, S151, Q145, Y147, R146, 1131
R153, S151, Q145, Y147, R146, G144
R153, S151, Q145, Y147, R146, L142
Epi#05
G3, A1, S183, T95, G101, A100, S96, G97
G3, A1, F184, T93, G101, T95, S96, G97
G97, A100, S96, T95, G101, T93, S183, G3
Epi#06
G140, P160, D161, R158, K164, P165
G50, P137, D133, R146, Q145, P143
A162, P165, D161, R158, K164, P160
Epi#07
G148, T6, S181, D178, R170, P165, D58
G128, T6, S181, D178, R170, P165, D58
Epi#08
K44, D42, S45, A43, F41
Epi#09
A191, E192, R196, A195, R200, N25, N202, N.sub.2O.sub.6
D161, R158, D157, R153, N176, S151, N154
Epi#10
D161, A57, N34, A162, F159, R158, K164
D2, A1, R185, S183, F184, G3, R4
Epi#11
F41, F29, I38, Q36, D58
Epi#12
Y168, E155
Y90, E91
Epi#13
A63, W62, P165, T60, A162, P160, L142, G149, Y147
A63, W62, P165, T60, A162, P160, L142, G128, Y147
A63, W169, P165, T60, A162, P160, L142, G144, Y147
Epi#15
P137, D133, I131, R146, G144
P137, D133, I131, R146, G148
P137, D133, I131, R146, G130
P137, D133, I131, R146, G128
P137, D133, I131, R146, G149
Epi#16
Q138, P137, Y54, R37, Q36, N34, A162, A57, D161
R170, P165, Y168, R153, S151, N176, D172, A63, D67
R170, P165, Y168, R153, S151, N176, D172, A63, D66
Epi#17
A1, S183, R4, S117
A100, S181, R4, S183
A1, S183, R4, S5
Epi#18
N118, R4, S181, - - - , G3, - - - , S117, L115, - - - , A78,
S80
N34, N32, R37, F35, - - - , A33, Y54, S45, - - - , - - - , A43,
V52
Epi#19
D157, N154, S151, Q145, R146, L142
D178, N176, S151, Q145, R146, G144
Epi#22
D40, A43, D42, W18, K20
D40, A43, D42, A19, K20
Epi#23
R158, N154, E155, L142, Q145, P143
R153, N154, E155, S151, Q145, P143
Epi#24
D42, K44, E48, P137, F139, A33, Q36
D40, K44, E48, P137, F139, A33, Q36
D161, K164, A162, P160, R158, L142, Q145
D161, K164, E155, P143, R158, L142, Q145
Epi#25
R158, K164, W169, D172, N176
R4, H119, 177, E82, N81
Epi#26
W18, S15, E82, W85, P23, A19, D42
W18, S15, E82, W85, P23, G84, D203
Epi#28
I31, D133, Q138, L142, E155, K164, F159, P165, D161
I131, D133, Q138, L142, E155, K164, F159, P143, R158
I131, D133, Q138, L142, E155, K164, F159, P160, R158
Epi#29
I131, R146, L142, R158, E155
G144, Q145, L142, R158, E155
Epi#30
G79, N81, A78, H119, S117, I77, L115
G79, N81, A78, H119, S76, I77, L115
Epi#31
L142, R158, N154, R153, W169, F171, D172
Epi#33
Q36, F29, P27, S15, A19, K20
K44, F41, P27, S15, A19, K20
Epi#34
V129, P143, S151, G144, R146, Y147, T6
V129, P143, S151, G148, R146, Y147, T6
V129, P143, S151, G149, R146, Y147, T6
Epi#36
A83, A22, A19, S15, K13, V52, A43, W18
Epi#37
Y147, R146, L142, R158, D161
Y147, R146, L142, R158, N154
Y147, R146, L142, R158, D157
Epi#38
E155, R158, P160, G140, L142
E155, R158, P143, G144, L142
Epi#40
G79, L115, G113, T111, A74, T6
G79, L115, G113, T111, A74, S15
G79, L115, G113, T111, A74, S110
G116, L115, G113, T111, A74, T6
G79, L115, G113, T111, A74, S76
Epi#42
L142, P143, S151, G144, R146, Q145
L142, P143, S151, G148, R146, Q145
L142, P143, S151, G149, R146, Q145
Epi#44
L142, R158, D161, P165, W62, Y168, S152, G144, P143
I131, R146, D133, P137, Y54, A33, V52, P49
L142, R158, D161, P165, W62, Y168, S152, G149, P143
Epi#45
R185, P208, F207, N206, D203, V24
D67, P213, F68, N65, D66, V64
R185, P208, F207, N206, D204, G205
Epi#46
A195, R200, R201, P23, N202, G205
A191, R200, R201, P23, N202, G205
V24, R201, R200, P190, Q211, A209
Epi#47
A191, A195, E192, V194, R200, N202, R201, P23
A195, A191, E192, V194, R200, N25, R201, P23
A191, A195, R196, V194, R200, N202, R201, P23
Epi#48
E48, K44, P49, P137, V52
E48, K44, P49, P137, G50
E48, K44, P49, P137, G140
Epi#50
D172, Y168, W62, V64, P213
D42, W18, A43, T39
D67, W173, W62, V64, P213
D66, W173, W62, V64, P213
D42, W18, S45, P49
D172, W169, W62, V64, P213
Epi#51
R4, H119, D2, T95, P98, K175, W169
R4, H119, D2, R185, P208, Q186, W85
R4, H119, D2, T95, G97, K175, W173
Epi#52
W18, A22, R200, R201, W85, Q186
Esperase:
Epi#01
N24, P239, R237, K235, N243, S240, Q245, T242
N24, P239, K235, R27, N117, Y91, R43, S87
N24, P239, R237, K235, N243, Y241, Q245, S240
Epi#02
T3, N76, L75, R43, S38, Y209, R213, V215
T3, N76, S87, R43, S38, Y209, R213, V215
T129, N166, Q161, R160, T156, Y192, R186, V203
Epi#03
R186, Y192, S261, Q161, R160, N155, G127
R186, Y192, S261, Q161, R160, N155, G157
R186, Y192, S261, Q161, R160, N155, L126
R186, Y192, S261, Q161, R160, T156, G162
R186, Y192, S261, Q161, R160, N155, A187
Epi#05
G102, A105, S133, T134, G131, R170, T129, Y167
G102, A105, S133, T134, G131, R170, T129, G127
G211, A37, R43, P40, G80, T3, S78, I79
Epi#06
G211, N61, D97, R98, S53, P55
G102, N99, D97, R98, S53, P55
G100, N99, D97, R98, S53, P55
Epi#07
211, T210, D60, S38, R43, P86, D89
Epi#08
A108, E136, S133, A105, F50
A108, E136, S132, A105, F50
A187, D181, S188, V203, F189
Epi#09
N212, G211, S38, H59, N61, N99, R98
S52, S53, R98, N99, N61, G211
Epi#10
T129, T156, N155, S188, F189, G157, R160
D181, N183, R186, S188, F189, G157, R160
T129, N166, N155, S188, F189, G157, R160
T129, T156, N155, S218, F189, G157, R160
D97, N99, N61, S57, F50, G102, R98
Epi#12
Y167, E136
Y192, E195
Y171, E136
Epi#13
S38, R43, P40, A37, H59, S57, P55, Y58
S38, R43, P40, A37, H59, S57, P55, F50
S38, R43, P40, A37, H59, S49, P55, Y58
Epi#15
N24, P86, D89, I44, R43, A45
N24, P86, D89, I44, R43, G46
N76, P86, D89, I44, R43, A45
N24, P86, D89, I44, R43, A37
Epi#16
Q161, P194, Y192, G157, R160, S188, D181, A187, N183
Q161, P194, Y192, R186, Q185, S188, D181, A187, N183
Q161, P194, Y192, G162, R160, S188, D181, A187, N155
Epi#17
A37, S38, R43, S87
Epi#18
N144, N140, R141, L137, S133, T134, E136, S132
N140, N144, R141, L137, S133, T134, A105, S103
N143, N144, R141, L137, S133, T134, E136, N140
Epi#19
I21, N18, Q15, Q275, R19, G20
I21, N18, Q15, Q275, R237, G20
E197, N265, S261, Q161, R160, G162
E197, N265, S261, Q161, R160, G157
I21, N18, Q15, Q275, R237, G25
Epi#23
R98, N61, E54, S53, F50, P55
R98, N61, E54, Y58, F50, P55
R98, N61, E54, S57, F50, P55
R98, N61, E54, S52, F50, A105
Epi#24
E195, G264, E197, P260, S261, P194, Q161
D89, G46, A48, P55, S52, F50, Q109
E197, G264, E195, P194, S261, L262, Q161
Epi#25
R98, H59, E54, N61
R98, H59, D60, N61
R43, H39, I44, D89, N24
R27, H120,1115, E112, N116
Epi#28
L104, Q109, I115, E112, W113, F50, S53, R98
A105, Q109, I115, E112, W113, F50, G102, R98
A108, Q109, I115, E112, W113, F50, S53, R98
V107, Q109, I115, E112, W113, F50, S53, R98
Epi#29
I147, N140, L137, R141, E136
G146, N140, L137, R141, E112
I115, N143, L137, R141, E136
G102, N99, L96, R98, E54
Epi#30
G211, N212, S38, H59, S57, I51, P55
G211, N61, S57, H59, S38, P40, L75
G211, N212, S38, H59, S49, I51, P55
G211, N212, S38, H59, P55, I51, L96
Epi#31
L257, Q185, N183, R186, F189, V203, D181
L262, Q185, N183, R186, F189, V203, D181
Epi#33
H59, Y58, P55, S52, S53, R98
Q109, F50, P55, S57, S53, R98
Q109, F50, P55, S49, S53, R98
Epi#34
I79, P40, S38, G211, R213, Y209, S216
I79, P40, S38, G211, R213, Y214, T210
I51, P55, S49, L96, R98, S53, S52
Epi#37
T134, A108, L137, R141, N144
Y256, A254, L257, R186, N183
A105, A108, L137, R141, N144
Epi#38
L257, G264, E195, L262, N265, P260, S259
L257, G264, E195, L262, N265, P260, S261
Epi#39
E195, R170, P194, G264, L257
E195, R170, P194, G264, L262
Epi#40
R141, L137, A108, T134, A105, S133
R43, L42, A37, Y58, P55, S52
R186, L257, A254, Y256, P260, S259
R186, L262, G258, Y256, P260, S259
R186, L257, G184, Y256, P260, S259
R141, L137, A108, T134, A105, S103
R186, L262, G264, Y256, P260, S259
R186, L257, A254, Y256, P260, S261
R186, L262, G258, Y256, P260, S261
R186, L257, G264, Y256, P260, S261
Epi#41
P260, Y256, L257, S259
Epi#42
L75, P86, S87, N24, P239, R237, Q275
L75, P86, S87, N24, P239, R237, R19
Epi#44
S53, R98, D97, Y58, S57, A48, P55
S53, R98, D97, Y58, S38, G211, T210
Epi#45
R19, H17, F22, N24, D89, G25
R43, P86, F22, N24, D89, G25
R272, H269, F10, N183, D181, V203
R272, H269, F10, N183, D181, G184
R43, P86, F22, N24, D89, G46
Epi#46
R19, R237, P239, N24, G20
R19, R237, P239, N24, G25
Epi#47
G162, Y192, R160, N155, A187, Q185, N183, R186, S188
G157, Y192, R160, N155, A187, Q185, N183, R186, S188
S261, Y192, R160, N155, A187, Q182, N183, R186, S188
L262, Y192, R160, N155, A187, Q182, N183, R186, S188
Epi#48
S261, Q161, P194, P260, G258
S261, Q161, P194, P260, G264
Epi#50
D181, W6, V4, T3
D181, W6, V203, S188
D181, W6, V4, S9
D181, W6, T3, P5
Epi#51
R98, H64, T210, R213, P40, S38, H59
R98, H64, T210, R213, G211, S38, H59
R19, H17, Q15, Q275, R272, Q252, H269
Laccase:
Epi#02
A14, N15, S17, F21, P180, Y176, R266, V177
T22, N15, P18, F21, P180, Y176, R266, V177
A274, N275, A181, R175, P180, Y176, R266, V177
A24, N15, S17, F21, P180, Y176, R266, V177
T272, N275, A181, R175, P180, Y176, R266, V177
Epi#03
L184, K173, I186, Y256
Epi#04
R234, S211, Q261, K264, N267, G271
R234, S211, Q261, K264, R266, G268,
R259, S211, Q302, R234, N299, A301
R259, S211, Q236, R234, N299, A301
Epi#05
G372, A371, L369, P350, G81, S349, S351, V352
G372, A371, L369, P350, G81, S351, S349, Y347
Epi#06
G286, N289, D291, T293, S295, P292
G214, P252, D254, T293, S295, P298
A288, N289, D291, T293, S295, P292
Epi#07
G214, T294, D291, R283, V253, P252, D254
G30, T12, D53, R59, A497, P89, D51
G30, T10, D51, R59, A497, P55, D53
Epi#08
A371, E348, S349, A346, F335
A14, D53, G90, A92, H91, F93
A181, E183, G20, V16, F21
A181, E183, G20, A182, F21
Epi#09
N41, A100, N43, V6, D42, R37, N4, T8, L94
N41, A100, N43, V6, D42, R37, N4, T8, N47
L369, N366, E376, R379, N472, A471, V474
Epi#10
E183, A181, N275, T272, F273, G268, R266
D129, N41, N43, A100, F69, G72, R71
E183, A181, N275, A274, F273, G271, K264
Epi#11
F93, L486, I489, Q485, V481, E482
Epi#12
Y490, E488
Y375, E376
Epi#13
N366, P370, D367, I358, Q363, A471
N366, P370, D367, I358, Q363, G361
R379, P378, D326, I319, T321, G323
R379, P378, D326, I319, T321, G318
R379, P378, D326, I319, T321, A324
Epi#15
N366, P370, D367, I358, Q363, A471
N366, P370, D367, I358, Q363, G361
R379, P378, D326, I319, T321, G323
R379, P378, D326, I319, T321, G318
R379, P378, D326, I319, T321, A324
Epi#16
R175, P180, Y176, R266, Q164, N267, D166, A163, D205
R283, P292, Y256, G214, Q251, D254, A285, A288, N289
R283, P292, Y256, G214, Q251, D254, D291, A290, N289
Epi#17
A306, S413, R409, S414
A411, S413, R409, S414
A306, S410, R409, S414
A411, S414, R409, S410
Epi#19
E216, N250, Q251, Q191, R283, G286
E190, N250, Q251, Q191, R283, A288
E216, N250, Q251, Q191, R283, A290
E190, N250, Q251, Q191, R283, A285
Epi#22
D491, P494, D492, P495, E496
D492, P494, D491, L493, E496
Epi#23
R339, N460, E348, S349, L369, A371
R339, N460, E348, S351, L369, P370
R339, N460, E348, S351, L369, A365
R339, N460, E348, S351, L369, P350
R283, N188, E190, N250, Q191, P252
Epi#24
D475, G72, A476, P445, R379, A471, Q363
D53, G90, A497, P495, T498, P55, Q501
D53, G90, A497, P495, S499, L58, Q501
Epi#25
R37, K40, D129, N130
R37, K40, D129, N41
Epi#27
E142, E139, D138, K194,
E142, E139, D138, K193
Epi#28
L58, Q501, I500, E496, L493, P495, D492
G286, D254, Q191, K194, E190, K193, G192, D138
A288, D254, Q191, K193, E190, K194, G192, D138
G192, D248, Q191, K194, E139, L136, A135, D138
V253, D254, Q191, K193, E190, K194, G192, D138
A285, D254, Q191, K193, E190, K194, G192, D138
Epi#29
G390, Q332, L329, R330, E435
V374, N366, L369, E348
I500, P495, L493, E496
G344, Q332, L333, R330, E435
Epi#30
G412, N304, A306, H309, I312, P314, V419
I312, L311, A315, H309, P229, L136, P132
Epi#31
L329, Q332, N343, R330, F331, V386, D434
L333, Q332, N343, R330, F331, V386, D434
L58, Q501, N54, R59, F112, M459, F456, D205
L58, Q501, N54, R59, F112, M459, I454, D205
Epi#33
Q485, Y490, P494, S499, A497, R59
Q251, Y256, P292, S295, A296, R234
H153, F21, V16, S17, A182, K173
H153, F21, P18, S17, A182, K173
Epi#34
V431, P395, T432, G433, G412, T415, S414
V431, P388, T432, G412, G433, S414, T415
V419, P320, T321, G323, P322, Y416, S414
V431, P395, T432, G390, G433, S414, T415
Epi#35
A371, L369, A362, S360, M359, I358
G372, L369, A362, S360, M359, I358
A365, L369, A362, S360, M359, I358
Epi#36
A362, A471, A476, V474, G361, S360, Q357, P350, A371, A365
A290, A288, A285, V253, Y256, S295, A296, W257
A288, A285, A287, V253, Y256, S295, A296, W257
Epi#37
P132, A135, L136, K194, N250
A135, A134, L136, K194, D138
P298, A301, L303, R234, N299
Epi#38
L356, G81, E348, A371, V374, L369, N366, P370, S351
L356, G81, E348, A371, V374, L369, N366, P370, S349
Epi#39
A411, E435, T432, P395, G393, L392
A1, E142, L35, R37, P34, G30, L27
A389, E435, T432, P395, G394, L392
Epi#40
R330, L333, G390, T432, A411, S414
G393, L392, G394, T432, A411, S414
R330, L333, G390, T432, A411, T415
Epi#41
P370, L369, V352, S351
P350, L369, V352, S351
Epi#42
L392, P395, S428, G430, P388, R330, Q332
Epi#44
S360, Q363, D367, P370, Y347, A371, G372, T345
V253, Q191, D254, P292, W257, Y256, S295, A296, P298
S360, Q363, D367, P370, Y347, S349, V352, P350
V253, Q191, D254, P292, W257, Y256, S295, G214, P252
Epi#45
R409, P322, F418, Y416, N420, D313, V419
K423, P314, F418, Y416, N420, D313, V419
R175, P180, F21, Y176, R266, D166, G268
Epi#46
A296, R259, R234, P300, N299, A301
Y256, R259, R234, P300, N299, Q302
Epi#47
I212, S211, R234, L303, A301, N299, P300, P298
I212, S211, R234, V232, A301, N299, P300, P298
Epi#48
S158, Q160, P157, P155, V504
S499, Q501, P55, P155, V504
E488, Q485, P480, P479, V481
Epi#49
D367, L369, V352, P350, Q357, Q363, M359, N478
D367, L369, P370, P350, Q357, Q363, M359, N478
Epi#50
D291, Y256, W257, S295, P298
D254, Y256, W257, T293, S295
Epi#51
D307, H309, E228, T218, P229, T231, H230
R234, H215, E216, T231, P229, H230, H309
D248, H215, E216, T231, P229, H230, H309
Epi#52
F69, A100, T98, R71, W75, T73, Q70
F97, A100, T98, R71, W75, T73, Q70
Natalase:
Epi#01
P344, P382, R387, R33, N32, S28, R31, T36
P344, P382, R387, R33, N29, S28, R31, T36
Epi#02
A87, N21, Q18, R24, S28, R31, R33
A87, K89, S83, R24, S28, R31, R33
Epi#03
L307, K305, H402, I404, Y398
L307, K305, H401, I404, Y398
L307, K305, A304, I404, Y398
Epi#04
R167, S166, Q168, R172, N171, I173
R177, Y131, S128, Q125, R123, N124, I127
Epi#05
G178, A180, N124, P120, G190, S187, H234, L195
G178, A180, N124, P120, G190, R123, S187, Y192
G178, A180, N124, P120, G190, S187, H234, Y192
Epi#06
A87, N21, D25, R24, Q18, P14
G145, N146, D150, T147, R144, P142
G143, N146, D150, T147, R144, P142
G450, N451, D447, T455, K452, P453
A87, N21, D25, R22, Q18, P14
G454, N451, D447, T455, K452, P453
A378, P382, D447, T455, K452, P453
Epi#07
G145, T147, D150, S149, R213, V208, P205, D201
Epi#08
K305, D400, A304, H402, F399
K305, D400, A304, H401, F399
Epi#09
S79, S83, D25, R22, R24, H86, N90, S28, R31
N439, A460, N459, V444, K478, N417, T413, T414
Epi#10
E254, N249, R248, T245, F239, R212, R213
E254, N249, R248, T245, F239, R241, K275
Epi#11
F169, I173, Q170, D162
L195, I173, Q170, D162
Epi#12
Y192, E188
Y357, E354
Epi#13
H12, L13, P369, A375, P374, S372, P330, W11
H12, L13, P369, A375, P374, S372, P330, L334
H12, L13, P369, A375, P374, S372, P330, G331
Epi#15
N451, P453, D447, I448, T449, A378
N451, P453, D447, I448, K452, G450
Epi#16
Q313, P316, Y357, R353, Q395, D397, D400, A304, N308
Q355, P316, Y357, G356, R353, D397, D400, A304, D302
Epi#17
A87, S83, R24, S28
A87, S28, R24, S83
Epi#18
R33, N32, R31, S28, G92, N90
Epi#19
D16, N50, S48, Q49, R72, G69
D25, N21, Q80, Q18, R24, A87
E82, T77, Q18, Q80, R72, G69
Epi#22
D461, A460, W463, W433
Epi#23
K478, N417, E410, N439, Q438, A460
K478, N417, E410, N439, Q438, A441
Epi#24
E332, G331, E335, P330, S372, A375, K379
D381, K379, A375, P369, S372, P374, K377
Epi#25
R154, K138, W136, D162, N171
R213, R212, W217, E216, N249
R154, K138, W136, E134, N112
R241, K236, W183, D203, E206
Epi#26
W163, S166, E134, W136, V161, E117, E126
W163, S166, E134, W136, V161, E117, D130
W163, S166, E134, W136, V161, E117, D162
Epi#27
D203, E206, D201, K236
E117, E126, D130, K175
D201, E206, D203, K179
E126, E117, D162, K175
Epi#28
L195, D162, Q168, W163, E134, W136, Q165, S166, R167
I173, D162, Q170, W163, E134, W136, Q165, S166, R167
V161, D162, Q170, W163, E134, W136, Q165, S166, R167
Epi#29
G331, P330, L334, F337, E335
G178, K175, L114, R177, E117
Epi#30
G450, N451, H446, K478, I448, P453
G454, N451, H446, K478, I448, P453
Epi#31
Q168, N171, R172, W163, M196, I173, D162
Q170, N171, R172, W163, V161, I173, D162
Epi#33
K377, Y366, P369, S372, A375, K379
K377, Y366, P374, S372, A375, K379
Epi#34
W433, W463, T457, V444, G454, T455, P453
W433, W463, T457, V456, G454, T455, P453
Epi#37
Y156, R177, L114, K175, D130
T132, R177, L114, K175, N124
Epi#38
G429, E431, N469, P428, S472
G430, E431, N469, P428, S472
Epi#39
E10, H12, T370, P330, G331, L334
E10, L13, T370, P330, G331, L334
Epi#40
A378, A375, Y366, P369, S372
R177, L114, G178, Y156, K138, T110
A375, A378, Y366, P369, T370
Epi#41
P369, L13, V52, S48
Epi#42
P316, S281, G356, R353, Q355
P316, S281, G356, R353, Q395
Epi#44
V208, R213, W217, Y148, S149, G145, P142
S28, R33, D381, Y365, A378, A375, P369
L13, D16, P14, W11, Y362, A375, V373, T370
S333, D327, P330, W11, Y362, A375, V373, P369
Epi#45
D108, P142, F65, Y60, N146, D150, G145
D140, P142, F65, Y60, N146, D150, G145
Epi#46
Y392, R387, R33, P382, G450, G454
Y392, R387, R33, P382, Q388, G3
Epi#47
S83, S79, E82, I85, R24, A87, N90, R31, S28
A250, G252, E254, N249, R248, F256, N279, R241, S238
Epi#48
S372, H371, P374, P369, V373
Epi#49
D51, W11, L13, V52, P14, Q18, Q80, T77, N21
D51, W11, L13, V52, P14, Q18, Q80, T77, K74
Epi#50
D461, Y435, W433, W463, T457
D400, Y398, W433, W463, T457
D397, Y435, W433, W463, T457
Epi#51
T394, H396, D397, D400, K305, H402, H401
T455, H446, K478, T457, G442, Q438, W463
Epi#52
W136, A109, E134, R167, W163, N171, Q170
W136, A109, E134, R167, W163, N171, Q168
PD498:
Epi#02
T262, K258, S260, F266, T198, Y196, R168, V166
T262, K258, S260, F266, T264, Y196, R168, V166
T141, N139, Q171, F170, S167, Y196, R168, V166
Epi#03
L99, K51, A49, I53, Y56
L99, K51, A49, I53, Y43
Epi#04
R28, S331, Q333, K97, R50, 153
R28, S331, Q333, K97, R50, A49
Epi#05
G108, A106, N107, G110, S109, S111, 159
G110, A106, N107, G108, S109, S111, L112
G108, A106, N107, G110, S111, S117, Y121
G108, A106, N107, G110, S111, S109, G135
G110, A106, L68, P214, G217, S219, Y220
G108, A106, N107, G110, S111, S109, L134
Epi#06
G135, N163, D164, R168, S174, P176
G162, N165, D164, R168, S174, P176
A22, N274, D25, S2, S9, P6
G154, N152, D148, T142, K144, P176
A22, P21, D25, S2, S9, P6
G154, N152, D148, S145, K144, P176
Epi#07
29, T332, S331, D95, S240, R28, V26, P21, D25
G29, T332, S330, D95, S331, R28, V26, P21, D25
Epi#08
K258, D257, S260, F266
K190, D185, S192, V207, F193
Epi#09
N215, N44, R50, 153, K54, N64, N63, R61
N44, A49, R50, 153, K54, N63, N64, R61
Epi#10
D188, N187, R189, S260, F266, G263, K258
D185, N187, R189, S260, F266, G263, K258
Epi#12
Y268, E253
Epi#15
R50, P46, D82, I87, T83, G86
N215, P46, D82, I87, T83, G86
Epi#18
N216, N44, R50, 153, A49, P46, N215
N215, N44, R50, 153, A49, P46, N216
Epi#19
D95, T332, S240, Q241, R28, G29
D95, T332, S330, Q241, R28, G29
Epi#22
D185, S192, D164, Y196, K267
D105, S111, D113, T141, K144
Epi#24
D95, K51, A49, P46, R50, K97
Epi#25
R120, K153, W151, D148, N152
R189, K190, D188, N187
R189, K190, D185, N208
Epi#27
D201, E253, D257, K258
D257, E253, D201, K267
Epi#28
I259, D257, Q254, E253, K267, F266, S260, R189
I259, D257, Q254, E253, K267, F266, S260, K258
Epi#29
L68, G108, L134, F170, E137
G135, N163, L134, F170, E137
Epi#30
G110, N107, A106, H71, L68, L104, L112
G108, N107, A106, H71, L68, P214, V213
G110, N107, A106, H71, P214, L68, L104
G110, N107, A106, H71, L68, L104, L134
Epi#33
Q12, Y220, V207, S222, S192, R189
190, F193, V207, S222, S192, R189
Q16, Y13, V207, S222, S192, R189
Epi#34
V26, W1, T27, G29, R28, S331, T332
W1, P21, T27, V26, R278, Y279, T255
Epi#35
G135, L134, S225, M221, I209
G110, L134, S225, M221, I209
G108, L134, S225, M221, I209
G162, L134, S225, M221, I209
Epi#37
A49, V52, L99, K54, N63
SAS: 309, Size 17.16: Y121, A127, L99, K54, N63
SAS: 307, Size 13.09: Y43, V52, L99, K54, N63
Epi#40
R189, G261, Y268, K258, S260
R189, G261, Y268, K258, T262
Epi#42
P3, S2, Q16, P21, R28, Q241
Epi#43
W199, Y196, G162, Q171, S140, L112, I115, T142
Epi#44
S145, D148, P176, W199, Y196, S167, G162, T169
S174, D201, P176, W199, Y196, S167, G197, T198
Epi#47
S330, S331, R28, V26, A22, Q16, N17, P21, S2
G242, S240, R28, V26, A22, Q16, N17, P21, S2
G29, S331, R28, V26, A22, Q16, N17, P21, S2
Epi#48
S2, D25, P21, P3, G86
S9, Q16, P21, P3, G86
Epi#50
R168, Y196, W199, T264, T198
D164, Y196, W199, T264, S260
Savinase:
L21, N18, P14, R19, K231, N232, S236, Q239, S234
L21, N18, P14, R19, K231, N232, S234, Q230, S24
L21, N18, P14, R19, K231, N232, S234, Q230, T22
Epi#02
T254, N255, A188, R164, S158, Y186, R180, V197
T249, N263, Q12, R10, P14, R19, R269
T249, N263, S9, R10, P14, R19, R269
[0594] Epi#03
K27, A86, I43, Y89
Epi#04
K229, S234, Q230, K231, R269, A266
K27, S24, Q230, K231, R269, A15
K231, S234, Q239, R241, N246, A248
Epi#05
G187, A188, N255, T254, G252, S250, T249, L251
G189, A188, N255, T254, G252, S250, T249, L261
Epi#06
G252, N179, D175, S182, S154, P127
A188, N255, D191, R164, S158, P127
A188, N255, D191, R164, S128, P127
Epi#08
A131, E134, S139, A106, F49
A166, E134, S139, A106, F49
Epi#09
S103, T132, A131, E134, A166, R164, N167, S142, R143
Epi#10
D175, N177, N179, S182, F183, G155, R180
D175, N212, N153, S182, F183, G155, R180
Epi#11
F49, L94, 1105, Q107, V102, E134
F49, K92, I105, Q107, V102, E134
Epi#12
Y161, E134
Y165, E134
Epi#13
S76, L73, P39, T207, A209, P204, S206, G205, Y208
S85, L73, P39, T207, A209, P204, S206, G205, Y203
Epi#16
R164, P127, Y161, G152, S158, N255, D191, A166, N167
R164, P129, Y161, G152, S158, N255, D191, A166, N138
Epi#17
A156, S158, R164, S128
A188, S158, R164, S126
Epi#18
N177, N179, R180, S182, G155, S154, A156, S158
N177, N178, R180, S182, G155, S154, N153, F183
Epi#19
D175, N179, S182, Q185, R180, L256
D175, N179, S182, Q185, R180, L251
I240, W235, S234, Q239, R241, K245
D175, N179, S182, Q185, R180, G252
Epi#23
R143, N114, E110, S139, Q135, A131
R143, N115, E110, N138, Q135, A131
Epi#24
D58, G59, E53, P51, F49, P54, Q57
D58, G59, E53, P51, S48, P54, Q57
D58, G59, E53, P54, S55, F49, Q107
Epi#25
R19, R269, E265, N18
R269, R19, E265, N18
Epi#28
V102, Q107, F49, E53, K92, Q57, G46, R44
A47, Q107, F49, E53, K92, Q57, G46, R44
V50, Q107, F49, E53, K92, Q57, G46, R44
Epi#29
I77, N74, L41, R44, E87
V4, N74, L41, R44, E87
G20, N18, L21, R19, E265
Epi#30
G59, N60, S97, H62, L94, P51, P54
G98, N60, S97, H62, L94, P51, P54
Epi#31
L256, R180, N178, R10, W6, V197, D175
L251, R180, N178, R10, W6, V197, D175
Epi#33
Q107, F49, P51, S48, S55, K92
Q107, F49, P54, S55, A47, K92
Epi#34
V102, P129, S128, G125, R164, Y161, P127
V102, P129, S126, G125, R164, S158, P127
[0595] Epi#37
T254, A188, L256, R180, N177
T254, A188, L256, R180, N179
Epi#38
L94, G59, E53, A96, N60, P204, S206
L94, G59, E53, A96, N60, P204, S36
Epi#39
A131, E134, L133, T132, P129, G125, L124
A166, E134, L133, T132, P129, G125, L124
Epi#40
R44, L41, G78, T207, P39, T37
R19, L21, G20, T22, K231, S234
R180, L256, G252, T254, A188, S158
Epi#41
P127, Y161, L133, V102, S99
P127, Y161, L133, V102, S103
P127, Y161, L133, V102, S101
P127, Y161, L133, V102, S126
Epi#42
L73, P84, S85, N74, H17, P14, R19, R269
L80, P5, S3, N74, H17, P14, R19, R269
L21, P84, S85, N74, H17, P14, R19, R269
Epi#43
105, W111, A47, G46, Q57, S36, L41, I43, T37
Epi#44
S126, R164, P127, Y161, S158, A188, T254
S128, R164, P129, Y161, S158, A188, T254
Epi#46
A15, R269, R19, P14, N18, G20
A266, R269, R19, P14, N18, A15
Epi#48
S55, Q57, P54, P51, G52
E53, Q57, P54, P51, G52
Epi#50
R10, W6, S3, S76
R241, W235, S234, P233
R10, W6, V4, S9
[0596] Epi#51
Q239, H243, T247, R269, R19, K231, W235
R19, H17, E265, R269, K231, S234, W235
Epi#52
A15, S9, R10, W6, N198, Q176
A15, S9, R10, W6, N198, Q200
Amylase SP722:
Epi#02
T419, N423, P422, F396, T5, Y398, R393, R37
T419, N418, P422, F396, T5, Y398, R393, R37
Epi#03
L313, K311, H408, I410, Y404
L313, K311, H407, I410, Y404
Epi#04
R171, S170, Q172, R176, N175, I177
R181, Y135, S132, Q129, R127, N128, I131
Epi#05
G184, A186, N128, P124, G196, S193, H240, L201
G184, A186, N128, P124, G196, R127, S193, Y198
Epi#06
G147, N150, D154, T151, R148, P146
G149, N150, D154, T151, R148, P146
Epi#07
G149, T151, D154, S153, R219, V214, P211, D207
Epi#08
K311, D406, A310, H407, F405
K311, D308, A310, H408, F405
Epi#09
T461, R485, K484, N423, T419, N418
R485, K484, N423, T420, T419
Epi#10
E260, N255, R254, T251, F245, R218, R219
T419, N423, N395, T5, F396, R393, R37
E260, T257, N255, T251, F245, R218, R219
Epi#11
F173, I177, Q174, D166
L201, I177, Q174, D166
Epi#12
Y363, E360
Y398, E360
Y198, E194
Epi#13
H16, L17, P375, A381, P380, S378, P336, W15
H16, L17, P375, A381, P380, S378, P336, G337
H16, L17, P375, A381, P380, S378, P336, L340
Epi#15
N457, P459, D453, I454, K458, G456
K458, P459, D453, I454, T455, A384
N457, P459, D453, I454, K458, G460
Epi#16
Q319, P322, Y363, R359, Q401, D403, D406, A310, N314
Q319, P322, Y363, G362, R359, D403, D406, A310, N314
Q319, P322, Y363, R359, R415, D403, D406, A310, N314
Epi#17
A91, S32, R28, S87
A91, S87, R82, S83
Epi#18
R485, V450, G448, T463, T461, H452, V462
N126, N128, R127, G196, Y198, S193, N195, N125
N25, R26, R28, S87, I89, A91, H90, N94
Epi#19
D20, N54, S52, Q53, R76, G73
D20, N19, Q22, Q84, R76, G73
D29, N25, Q22, Q84, R28, A91
Epi#20
K385, P350, L355, L313, K311, D308, G305, D432
Epi#22
D183, A186, D209, W189, K242
D183, A186, D209, W189, E190
D183, A186, D209, P211, E212
D209, A186, D183, Y160, W159
D183, A186, D209, W187, W189
Epi#23
R415, N418, E416, N445, Q444, A466
K446, N445, E416, Y441, Q444, A466
Epi#24
D387, K385, A381, P375, S378, P380, K383
E341, G337, E338, P336, S378, A381, K385
D333, G337, E341, P336, S378, A381, K385
Epi#25
R485, H452, I454, E391, N36
R485, K484, I454, E391, N395
Epi#26
W167, S170, E138, W140, V117, G182, D183
W167, S170, E138, W140, V165, E121, D134
W167, S170, E138, W140, V165, E121, E130
Epi#27
E212, E216, D154, K156
E216, E212, D209, K242
Epi#28
L201, D166, Q172, W167, E138, W140, Q169, S170, R171
L201, D166, Q169, W140, E138, W167, F173, S170, R171
L201, D166, Q174, W167, E138, W140, Q169, S170, R171
Epi#29
V214, N215, L217, R219, E222
G96, H90, L228, R82, E86
V214, R219, L217, R218, E212
Epi#30
G456, N457, H452, K484, I454, P459
G362, M323, S287, H324, K320, P322, V318
G362, M323, S287, H321, K320, P322, V318
G460, N457, H452, K484, I454, P459
Epi#31
L217, R219, N215, R218, F245, V214, D248
L217, R219, N215, R218, F245, M208, D209
Epi#33
K383, Y372, P375, S378, A381, K385
K383, Y372, P380, S378, A381, K38
Epi#34
W439, W469, T463, V450, R485, T461, P459
W439, W469, T463, V462, R485, T461, P459
Epi#37
T251, R218, L217, R219, N215
P211, V214, L217, R219, N215
A256, R218, L217, R219, N215
Epi#38
G435, E437, N475, P434, S478
G436, E437, N475, P434, S478
Epi#39
E338, H16, T376, P336, G337, L340
E14, H16, T376, P336, G337, L340
Epi#40
A384, A381, Y372, P375, S378
A384, A381, Y372, P375, T376
Epi#41
P375, L17, V56, S52
Epi#42
S378, P380, Y372, A381, A384, P375
S378, P375, Y372, A381, A384, P388
S378, P375, Y372, A381, A384, T455
Epi#45
K72, P146, F69, Y64, R148, D154, G149
K311, H408, F405, N409, D432, G304
D406, H408, F405, N409, D432, G304
Epi#46
Y398, R393, R37, P388, Q394, G7
Y398, R359, R393, P388, G456, G460
Y398, R393, R37, P388, Q394, G38
Epi#47
A256, G258, E260, N255, R254, F262, N285, R247, S244
S193, Y198, E194, N125, R127, Q129, N123, R176, P124
Epi#48
S378, H377, P380, P375, V379
H16, H377, P375, P380, V379
Epi#49
D55, W15, L17, P18, Q22, Q84, T81, N25
D55, W15, L17, P18, Q22, Q84, T81, K78
Epi#50
D467, Y441, W439, W469, T463
D406, Y404, W439, W469, T463
D183, Y160, W159, W140, T114
D403, Y441, W439, W469, T463
Epi#51
D406, H408, D308, K311, L313, Q319, H321
Epi#52
W140, A113, E138, R171, W167, N175, Q174
W140, A113, E138, R171, W167, D166, Q172
Amylase AA560:
Epi#01
L390, P388, P350, K383, K385, N457, S478, R458, T461
L390, P388, P350, K383, K385, N457, S478, R458, T452
L390, P388, P350, K383, K385, N457, S478, R458, T455
Epi#02
L390, K395, Q394, R393, T5, Y398, R359, R400
L173, K172, S170, T136, Y135, R118, R181
L173, R171, S170, T136, Y135, R118, R181
L390, K395, Q394, R393, T5, Y398, R400, R415
Epi#03
K438, H407, I410, Y404
Epi#04
K172, S170, Q169, R171, N174, L173
R171, S170, Q169, K172, N175, I177
Epi#05
G456, A459, R458, T461, G460, T452, T463, V450
G456, A459, R458, T452, G460, T461, T463, G448
Epi#06
A51, N54, D20, R76, Q71, P146
G73, A51, D55, S52, K72, P146
Epi#07
G456, T455, S384, D387, R393, P388, D453
Epi#08
K259, S255, V222, H252, F245
K259, G258, A256, H252, F245
Epi#09
N128, V131, R176, D166, K172, N175, N174, R171
Epi#10
467, N445, R444, F441, R415, R400
D467, A466, R444, F441, R415, R400
Epi#11
F69, K72, I75, Q53, V56, D55
Epi#12
Y16, E337
Y363, E360
Y198, E194
Epi#15
K385, P388, D453, I454, R458, A459
K385, P388, D387, I454, T452, A459
K385, P388, D387, I454, R458, G456
Epi#17
A87, S29, R28, S32
A91, S29, R28, S32
Epi#18
N445, R444, A466, T463, T461, N471, N437
N445, R444, A466, T463, T461, T452, V450
Epi#19
166, W167, S170, Q169, R171, K172
E138, W167, S170, Q169, R171, K172
E134, T136, S170, Q169, R171, K172
Epi#22
D209, P211, D207, Y160, D183
Epi#23
R400, N418, E416, N445, Q449, A466
R82, N83, E68, N70, F69, P146
Epi#24
E134, G133, E130, P124, R176, L173, K172
E134, K179, E130, P124, R176, L173, K172
Epi#25
R444, K446, W469, D467, N445
R171, K172, W167, D166, N175
R171, K172, W167, D166, N174
Epi#26
W167, S170, E138, W140, V165, E121, E130
W167, S170, E138, W140, V165, E121, E134
W167, S170, E138, W140, V165, E121, D166
Epi#27
E130, E121, D166, K172
D36, E391, D387, K385
E134, E121, D166, K172
Epi#28
L201, D166, Q169, W140, E138, K172, S170, R171
L173, D166, Q169, K172, E138, W167, S170, R171
Epi#29
V131, R176, L173, R171, E138
I177, N175, L173, R171, E138
I177, N174, L173, R171, E138
Epi#30
I39, N33, S29, H23, P18, L17, P375
G38, N33, S29, H23, L17, P375, P380
G362, M323, S287, H321, Q319, P322, V318
G417, N423, A420, H421, K395, L390, P388
G21, N25, S29, H23, P18, L17, P375
G399, N418, A420, H421, K395, L390, P388
Epi#31
L173, R171, N174, R176, W167, M202, I177, D166
L173, R171, N174, R176, W167, V165, I177, D166
Epi#33
K108, Y58, V56, S52, A51, K72
Epi#34
W439, W469, T463, V450, G460, T452, T461
W15, P18, T376, G378, P375, Y372, S384
W469, W439, S473, G460, R458, T461, T463
Epi#37
P124, R176, L173, K172, N175
P124, R176, L173, R171, N174
Epi#40
R400, G399, Y396, P422, T419
R400, G417, Y396, P422, T419
[0597] Epi#41
P375, Y16, L17, V56, S52
P18, Y16, L17, V56, S52
Epi#42
P350, S478, G433, H408, R310, Q311
P322, S287, N285, H324, R320, Q319
P322, S287, G362, H321, R320, Q319
Epi#44
L17, D20, P18, W15, Y368, A381, G378, T376
L340, D333, P336, W15, Y368, A381, G378, P375
Epi#45
K72, P146, F69, Y64, N150, D144, G147
D112, P146, F69, Y64, N150, D144, G149
Epi#46
Y398, R359, R393, P388, G456, A459
Y363, R359, R393, P388, Q394, G7
Y363, R359, R393, P388, Q394, G38
Epi#47
I75, E68, R76, N83, R82, Q84, N90, R28, S29
G133, E134, E130, V131, R176, L173, N174, R171, S170
Epi#48
S384, K383, P380, P375, G378
E337, H377, P380, P375, V379
Epi#50
R444, W469, W439, S473, T461
D183, Y160, W159, W140, T114
Epi#51
R320, H321, Q319, P322, H324, H286
Epi#52
W140, A113, E138, R171, W167, D166, Q169
W140, A113, E115, R118, W159, T114, Q169
Protease A:
Epi#01
L21, N18, P14, R19, K237, N238, S242, Q245, S240
L21, N18, P14, R19, K237, N238, S240, Q236, S24
Epi#02
T255, N269, Q12, R10, P14, R19, R275
T255, N269, S9, R10, P14, R19, R275
Epi#03
K27, A88, I44, Y91
Epi#04
K235, S240, Q236, K237, R275, A15
K27, S24, Q236, K237, R275, A15
K237, S240, Q245, R247, N252, A254
R145, S141, Q137, Y171, N173, A172
Epi#06
G61, N62, D60, T38, Q59, P55
G211, P210, D60, T38, Q59, P55
A98, N62, D60, T38, Q59, P55
G100, N62, D60, T38, Q59, P55
Epi#08
A131, E136, S141, A108, F50
A172, E136, S141, A108, F50
A98, E54, G53, V51, F50
Epi#09
S162, S170, A172, N173, V244, H249, N252, S256, T260
S259, S256, T260, N261, L262, R186, N185, S188, N155
S162, S170, A172, N173, V244, H249, N248, N252, T255
S156, S162, N261, S259, L262, R186, N185, S188, N155
Epi#10
D181, N183, N185, S188, F189, G157, R186
D181, N218, N155, S156, F189, G157, R186
Epi#12
Y171, E136
Y91, E89
Epi#13
S78, L75, P40, T213, A215, P210, S212, G211, Y209
S87, L75, P40, T213, A215, P210, S212, G211, Y214
Epi#16
L262, P194, Y192, G195, S162, N261, D197, A172, N140
L262, P194, Y192, G157, S162, N261, D197, A172, N173
L262, P194, Y192, G161, S162, S170, D197, A172, N173
Epi#17
A138, S141, R145, S144
A108, S141, R145, S144
Epi#18
N185, N183, R186, L262, S259, T260, P194, N261
N185, N183, R186, L262, Y192, T260, P194, S162
Epi#19
I246, W241, S240, Q245, R247, K251
D181, N185, S188, Q191, R186, L262
Epi#23
R145, N116, E112, S141, Q137, A138
R145, N117, E112, S141, Q137, A108
Epi#24
E136, G133, A131, P129, S103, F50, Q109
E136, G132, A131, P129, S103, A108, Q137
D60, G61, E54, P52, F50, P55, Q59
Epi#25
R275, R19, E271, N18
Epi#28
G20, H17, Q12, E271, L21, Q236, S240, K237
A15, H17, Q12, E271, L21, Q236, S240, K237
Epi#29
V244, Q245, L148, R145, E112
V244, N173, L148, R145, E112
Epi#30
G61, N62, A98, H64, L96, P52, P55
G20, N18, A15, H17, S87, L75, P40
I79, N76, S87, H17, Q12, P14, V4
G100, N62, A98, H64, L96, P52, P55
Epi#31
L262, R186, N184, R10, W6, V203, D181
L257, R186, N184, R10, W6, V203, D181
Epi#33
Q109, F50, P52, S49, S56, K94
Q109, F50, P55, S56, A48, K94
Epi#34
W241, P239, S242, G146, R145, S141, S144
I165, P194, T260, G258, R186, S188, S156
V104, P129, S130, G127, G102, S101, S99
V244, W241, S242, G146, R145, S141, S144
I165, P194, S170, G127, P129, S130, S103
Epi#37
P14, A15, L21, R19, N18
T143, R145, L148, R247, N252
T143, V244, L148, R145, N116
Epi#38
L96, G97, E54, A98, N62, P210, S212
L96, G97, E54, A98, N62, P210, S37
Epi#39
A15, E271, H17, R19, P14, G20, L21
A254, E271, H17, R19, P14, G20, L21
A272, E271, H17, R19, P14, G20, L21
Epi#40
R186, L257, G258, T260, P194, S162
R186, L262, G161, Y192, P194, T260
Epi#41
P194, Y192, L262, S259
P194, Y192, L196, S162
Epi#42
L82, P5, S3, N76, H17, P14, R19, R275
L82, P5, S9, Q12, H17, P14, R19, R275
Epi#43
W113, A48, G47, Q59, S37, L42, I44, T38
Epi#44
V244, R247, D197, P194, Y192, S162, G195, T260
V244, R247, D197, P194, Y192, S170, G195, T260
S56, Q59, D60, P210, Y214, S212, G211, T38
S56, Q59, D60, P210, Y209, S212, G211, T38
Epi#46
A15, R275, R19, P14, N18, G20
A272, R275, R19, P14, N18, G20
A272, R275, R19, P14, N18, A15
Epi#47
S130, A131, E136, N173, A172, N140, R145, S144
S105, A131, E136, N173, A172, N140, R145, S144
Epi#48
E54, Q59, P55, P52, G53
S56, Q59, P55, P52, G53
S49, Q59, P55, P52, G53
Epi#50
R10, W6, S3, S78
R10, W6, V4, S9
R10, W6, V203, S188
Epi#51
Q245, H249, T253, R275, K237, S240, W241
R19, H17, E271, R275, K237, S240, W241
R145, H120, K27, S24, K237, S240, W241
R145, H120, K235, K237, P239, S240, W241
Epi#52
A15, S9, R10, W6, N204, Q206
A15, S9, R10, W6, N204, Q182
Alcalase:
Epi#01
L10, P5, P9, K15, K12, N269, S251, R249, T253
L82, P5, P9, K15, K12, N269, S251, R249, T253
Epi#02
T115, N141, A144, R145, S242, R247, R249
A138, N141, A144, R145, S242, R247, R249
Epi#03
L196, K170, A129, 1165, Y167
L196, K170, A194, I165, Y171
Epi#04
R145, Y143, S173, Q137, K136, T133, A134
K170, Y167, S132, Q137, K136, N141, A144
Epi#05
G53, A52, F50, G102, S105, S103, Y104
G53, A52, F50, G102, S101, S103, Y104
Epi#06
A24, N25, D120, R145, S242, P239
A144, N141, D140, R145, S242, P239
Epi#08
K265, E197, S260, A194, F261
A56, E54, G53, A52, F50
Epi#10
T162, N161, N163, A194, F261, G264, K265
E195, N161, N163, S158, F261, G258, K265
Epi#12
Y57, E54
Y262, E197
Epi#13
S38, A37, P40, T213, A215, H64, L217, G204, Y206
S38, A37, P40, T213, A215, H64, S98, G100, G61
S87, L75, P40, T213, A215, H64, L217, G204, Y6
Epi#16
L10, P9, Y6, G204, S182, N183, D181, A187, N185
Q2, P5, Y206, G204, S182, N183, D181, A203, N218
L10, P9, Y6, G204, S182, N183, D181, A187, N155
Epi#17
A144, S244, R247, S252
A272, S252, R249, S244
A144, S244, R249, S251
A254, S252, R249, S244
Epi#18
N141, R145, A144, Y143, S244, N248, S252
Epi#19
N248, S244, Q245, R249, A272
N240, S242, Q245, R249, A254
N240, S242, Q245, R249, L241
Epi#22
D76, L82, D14, A18, K15
D181, L10, D14, A18, K15
Epi#23
K27, N117, E112, N141, Q137, A134
K27, N117, E112, N141, Q137, A138
K27, N117, E112, S109, F50, A52
Epi#24
D120, K27, A24, P86, F21, A18, K15
D14, K22, A24, P86, F21, A18, K15
D76, K22, A24, P86, S87, F21, K15
Epi#25
R249, R247, E197, E195
Epi#27
D172, E195, E197, K265
E197, E195, D172, K136
D172, E197, E195, K170
Epi#28
A18, D14, Q19, K15, E271, K12, Q17, S87, D76
V4, D14, Q17, K12, E271, K15, F21, A18, K22
Epi#29
L257, K265, L196, F261, E195
G53, N97, L96, F50, E54
Epi#30
G146, L241, S242, H238, K237, P239, L235
G146, L241, S236, H238, S242, P239, L235
Epi#33
K15, F21, P86, S87, A24, K27
K27, Y91, V45, S89, A24, K22
Epi#34
V4, P5, T3, G80, P40, S38, T211
V108, W113, T116, G118, R145, Y143, S244
V26, P239, S242, G146, R145, T115, T116
Epi#36
A52, A56, A48, V51, G102, Y104, S105, V108, A138, A134
A52, A56, A48, V51, G102, Y104, S103, V108, A134, A138
Epi#37
Y262, A194, L196, K265, Y256
Y263, R186, L257, K265, Y256
Y256, A254, L257, K265, Y262
Epi#40
R186, L257, A254, Y256, K265, S252
R186, L257, G258, Y256, K265, S260
Epi#41
Y256, L257, S260
Y256, L257, S259
Epi#42
L235, P239, S242, N248, R249, Q275
L241, P239, S242, Q245, R249, Q275
Epi#44
S132, Q137, D140, Y143, A144, A138, T133
V108, Q137, D140, Y143, A144, A138, T133
S173, Q137, D140, Y143, A144, A138, T133
Epi#48
Q19, K15, P9, P5, V4
E271, K15, P9, P5, V4
Protease B:
Epi#05
SAS: 454, Size 24.86: G189, A188, R164, P127, G125, S99
SAS: 452, Size 15.92: G189, A188, R164, P127, G125, S128
SAS: 451, Size 24.86: G157, A188, R164, P127, G125, S99
SAS: 449, Size 15.92: G157, A188, R164, P127, G125, S128
SAS: 445, Size 23.31: G189, A166, R164, P127, G125, S99
Epi#09
SAS: 446, Size 15.76: T254, G189, A166, R164, A188, S158
SAS: 312, Size 15.90: T22, G20, L21, R19, A15, S9
Epi#10
SAS: 460, Size 17.32: D175, N177, N179, S182, F183, G155, R180
SAS: 437, Size 16.70: D211, N212, N153, S182, F183, G155, R180
SAS: 424, Size 13.75: D175, N212, N153, S182, F183, G155, R180
SAS: 417, Size 16.70: D211, N212, N153, S154, F183, G155, R180
SAS: 404, Size 15.83: D175, N212, N153, S154, F183, G155, R180
Epi#12
SAS: 309, Size 13.46: P127, Y161, E134, P129
SAS: 292, Size 9.37: R164, Y161, E134, P129
SAS: 287, Size 18.66: P127, Y161, E134, N138
SAS: 284, Size 16.85: P127, Y161, E134, N167
SAS: 275, Size 11.53: S128, Y161, E134, P129
Epi#17
SAS: 275, Size 15.84: A188, S158, R164, S126
SAS: 225, Size 12.79: A156, S158, R164, S126
Epi#18
SAS: 444, Size 16.32: S250, K245, S259, L256, A188, T254, L251
SAS: 397, Size 14.14: S250, K245, S259, L256, G252, T254, L251
SAS: 397, Size 14.14: S250, K245, S259, L251, G252, T254, L256
SAS: 397, Size 14.14: S259, K245, S250, L251, G252, T254, L256
SAS: 396, Size 21.52: S158, R164, S126, V102, G100, S99, L124
Epi#19
SAS: 295, Size 15.06: D175, W6, S9, Q12, R10
SAS: 278, Size 21.23: E110, T141, S236, Q239, R241
Epi#23
SAS: 486, Size 19.88: R143, N114, E110, S139, Q135, A131
SAS: 473, Size 18.68: R19, N18, E265, L21, Q230, P233
SAS: 468, Size 15.74: R164, N167, E134, S139, Q135, A131
SAS: 463, Size 13.77: R164, N167, E134, S130, Q135, A131
SAS: 461, Size 21.98: R44, N42, E87, S24, Q230, P233
Epi#28
SAS: 520, Size 19.27: V102, Q107, W111, E110, Q135, S139, R143
SAS: 492, Size 24.70: V102, Q107, F49, E53, Q57, G46, R44
SAS: 480, Size 22.76: V50, Q107, W111, E110, Q135, S139, R143
SAS: 452, Size 19.08: V50, Q107, F49, E53, Q57, G46, R44
SAS: 441, Size 24.70: V102, Q107, E110, W111, F49, G46, R44
Epi#29
SAS: 239, Size 11.49: G20, N18, L21, E265
SAS: 224, Size 11.49: G20, R19, L21, E265
SAS: 179, Size 16.62: 14, P14, L21, E265
SAS: 175, Size 11.49: G20, K231, L21, E265
SAS: 153, Size 18.96: G25, Q230, L21, E265
Epi#30
SAS: 308, Size 24.27: G20, L21, A15, H17, S85, L73, P39
Epi#31
SAS: 363, Size 21.72: L256, R180, N178, R10, W6, V197, D211
SAS: 352, Size 22.95: L251, R180, N178, R10, W6, V197, D211
SAS: 350, Size 21.62: L256, R180, N178, R10, W6, V197, D175
SAS: 339, Size 17.75: L251, R180, N178, R10, W6, V197, D175
Epi#34
SAS: 430, Size 18.33: V238, W235, S236, G144, R143, S139, S142
SAS: 430, Size 18.33: V238, W235, S236, G144, R143, S142, S139
SAS: 420, Size 13.98: V238, W235, S236, G144, R143, S142, T141
SAS: 420, Size 13.98: V238, W235, S236, G144, R143, T141, S142
SAS: 352, Size 18.33: V238, W235, S236, G144, R143, S139, T141
Epi#37
SAS: 415, Size 23.06: T254, A188, L256, R180, N177
SAS: 374, Size 18.08: T254, A188, L256, R180, N179
SAS: 335, Size 19.96: T254, A188, L256, R180, N178
Epi#39
SAS: 425, Size 16.00: A166, E134, R164, P127, G125, L124
SAS: 421, Size 16.36: A131, E134, R164, P127, G125, L124
SAS: 400, Size 16.00: A166, E134, R164, P129, G125, L124
SAS: 396, Size 16.36: A131, E134, R164, P129, G125, L124
SAS: 359, Size 16.00: A166, E134, T132, P129, G125, L124
Epi#40
SAS: 358, Size 15.76: A166, G189, Y186, A188, T254
SAS: 352, Size 15.76: A166, G189, T254, A188, S158
SAS: 326, Size 11.62: A96, G59, T56, P54, S55
SAS: 322, Size 15.30: G98, G59, T56, P54, S55
SAS: 318, Size 17.81: A188, G189, Y186, A156, S182
Epi#42
SAS: 528, Size 16.22: L21, P14, S9, Q12, H17, R19, R269
Epi#44
SAS: 401, Size 15.10: L256, R180, Y186, S158, A188, T254
SAS: 393, Size 15.52: L256, R180, Y186, A188, G189, T254
SAS: 390, Size 18.46: L251, R180, Y186, S158, A188, T254
SAS: 382, Size 16.23: L251, R180, Y186, A188, G189, T254
SAS: 376, Size 22.23: V197, R180, Y186, S158, A188, T254
Epi#46
SAS: 559, Size 12.63: A15, R269, R19, P14, N18, G20
Epi#53
SAS: 298, Size 9.48: W235, S234, Q230, K231
SAS: 298, Size 18.05: W235, S234, Q239, K245
SAS: 289, Size 9.48: W235, P233, Q230, K231
SAS: 283, Size 9.61: W235, S234, Q239, K229
SAS: 255, Size 14.51: W235, S236, Q239, K245
ProteaseC:
Epi#05
SAS: 445, Size 23.34: G189, A166, R164, P127, G125, S99
SAS: 445, Size 24.90: G189, A188, R164, P127, G125, S99
SAS: 433, Size 24.90: G157, A188, R164, P127, G125, S99
SAS: 427, Size 15.89: G189, A188, R164, P127, G125, S128
SAS: 427, Size 15.50: G189, A166, R164, P127, G125, S128
Epi#09
SAS: 463, Size 15.74: T254, G189, A166, R164, A188, S158
SAS: 425, Size 15.74: D191, G189, A166, R164, A188, T254
SAS: 384, Size 13.57: D191, G189, A166, R164, A188, S158
Epi#10
SAS: 445, Size 17.28: D175, N177, N179, S182, F183, G155, R180
SAS: 431, Size 13.75: D175, N212, N153, S182, F183, G155, R180
SAS: 403, Size 15.83: D175, N212, N153, S154, F183, G155, R180
SAS: 387, Size 16.14: D175, N178, N179, S182, F183, G155, R180
SAS: 373, Size 16.76: D175, N212, N153, A156, F183, G155, R180
Epi#12
SAS: 292, Size 13.45: P127, Y161, E134, P129
SAS: 287, Size 9.30: R44, Y89, E87, N42
SAS: 284, Size 9.35: R164, Y161, E134, P129
SAS: 282, Size 9.35: R164, Y165, E134, P129
SAS: 272, Size 16.85: P127, Y161, E134, N167
Epi#16
SAS: 547, Size 20.59: R164, P129, Y165, G189, S158, N255, D191,
A166, N167
SAS: 543, Size 23.80: R164, P129, Y165, G189, S158, N255, D191,
A166, N138
Epi#17
SAS: 267, Size 15.84: A188, S158, R164, S126
SAS: 231, Size 12.82: A156, S158, R164, S126
Epi#18
SAS: 449, Size 16.85: S182, R180, L256, A188, T254, L251
SAS: 426, Size 21.97: S126, R164, S158, A188, T254, L256
SAS: 407, Size 15.92: S182, R180, L251, G252, T254, L256
SAS: 407, Size 15.92: S182, R180, L256, G252, T254, L251
SAS: 391, Size 18.26: S182, R180, L256, G252, S250, L251
Epi#19
SAS: 293, Size 15.04: D175, W6, S9, Q12, R10
SAS: 291, Size 17.13: D191, N242, S236, Q239, R241
SAS: 273, Size 21.24: E110, T141, S236, Q239, R241
Epi#23
SAS: 463, Size 19.84: R143, N114, E110, S139, Q135, A131
SAS: 451, Size 15.68: R164, N167, E134, S139, Q135, A131
SAS: 443, Size 21.95: R44, N42, E87, S24, Q230, P233
SAS: 440, Size 22.70: R143, N115, E110, S139, Q135, A131
SAS: 431, Size 15.11: R44, N42, E87, S85, L73, P39
Epi#28
SAS: 402, Size 18.79: G59, Q57, E53, F49, G46, R44
SAS: 384, Size 20.81: A96, Q57, E53, F49, G46, R44
SAS: 376, Size 18.79: A47, Q57, E53, F49, G46, R44
Epi#31
SAS: 348, Size 21.63: L256, R180, N178, R10, W6, V197, D175
SAS: 342, Size 17.75: L251, R180, N178, R10, W6, V197, D175
Epi#33
SAS: 399, Size 18.88: Q107, Y102, P129, S126, R164
SAS: 355, Size 15.95: Q135, Y165, P129, S126, R164
Epi#34
SAS: 424, Size 18.37: V238, W235, S236, G144, R143, S139, S142
SAS: 424, Size 18.37: V238, W235, S236, G144, R143, S142, S139
SAS: 408, Size 14.02: V238, W235, S236, G144, R143, S142, T141
SAS: 408, Size 14.02: V238, W235, S236, G144, R143, T141, S142
SAS: 346, Size 18.37: V238, W235, S236, G144, R143, T141, S139
Epi#37
SAS: 405, Size 23.05: T254, A188, L256, R180, N177
SAS: 364, Size 18.08: T254, A188, L256, R180, N179
SAS: 347, Size 19.96: T254, A188, L256, R180, N178
Epi#40
SAS: 368, Size 15.74: A166, G189, T254, A188, S158
SAS: 362, Size 15.74: A166, G189, Y186, A188, T254
SAS: 326, Size 17.80: A188, G189, Y186, A156, S182
SAS: 326, Size 23.72: A166, G189, Y186, A156, S182
SAS: 326, Size 17.80: G189, A188, Y186, A156, S182
Epi#41
SAS: 232, Size 19.49: P204, Y208, L211, V197, S210
Epi#44
SAS: 445, Size 22.71: V238, R241, D191, Y186, S158, A188, T254
SAS: 429, Size 21.14: V238, R241, D191, Y186, A188, G189, T254
SAS: 410, Size 22.71: V238, R241, D191, Y186, S158, G189, T254
SAS: 404, Size 23.33: V238, R241, D191, Y257, S250, G252, T254
SAS: 382, Size 23.33: V238, R241, D191, Y257, S253, G252, T254
Epi#46
SAS: 567, Size 12.67: A15, R269, R19, P14, N18, G20
Epi#53
SAS: 305, Size 9.43: W235, S234, Q230, K231
SAS: 303, Size 9.53: W235, S234, Q239, K229
SAS: 276, Size 9.43: W235, P233, Q230, K231
SAS: 259, Size 9.43: W235, S234, Q230, K229
SAS: 233, Size 9.53: W235, S236, Q239, K229
ProteaseD:
Epi#05
SAS: 453, Size 24.94: G189, A188, R164, P127, G125, S99
SAS: 449, Size 23.37: G189, A166, R164, P127, G125, S99
SAS: 442, Size 24.94: G157, A188, R164, P127, G125, S99
SAS: 439, Size 15.91: G189, A188, R164, P127, G125, S128
SAS: 435, Size 15.50: G189, A166, R164, P127, G125, S128
Epi#09
SAS: 448, Size 15.77: T254, G189, A166, R164, A188, S158
Epi#10
SAS: 460, Size 17.32: D175, N177, N179, S182, F183, G155, R180
SAS: 428, Size 13.76: D175, N212, N153, S182, F183, G155, R180
SAS: 403, Size 15.83: D175, N212, N153, S154, F183, G155, R180
SAS: 391, Size 16.15: D175, N178, N179, S182, F183, G155, R180
SAS: 372, Size 16.77: D175, N212, N153, A156, F183, G155, R180
Epi#12
SAS: 302, Size 13.47: P127, Y161, E134, P129
SAS: 290, Size 9.39: R164, Y161, E134, P129
SAS: 282, Size 18.68: P127, Y161, E134, N138
SAS: 280, Size 16.87: P127, Y161, E134, N167
SAS: 270, Size 13.10: R164, Y161, E134, N138
Epi#17
SAS: 286, Size 15.87: A188, S158, R164, S126
SAS: 250, Size 12.76: A156, S158, R164, S126
Epi#18
SAS: 446, Size 16.31: S250, K245, S259, L256, A188, T254, L251
SAS: 406, Size 14.13: S250, K245, S259, L256, G252, T254, L251
SAS: 406, Size 14.13: S250, K245, S259, L251, G252, T254, L256
SAS: 406, Size 14.13: S259, K245, S250, L251, G252, T254, L256
SAS: 388, Size 14.13: S250, K245, S259, L256, G252, T249, L251
Epi#19
SAS: 319, Size 15.07: D175, W6, S9, Q12, R10
SAS: 276, Size 21.28: E110, T141, S236, Q239, R241
Epi#23
SAS: 497, Size 19.86: R143, N114, E110, S139, Q135, A131
SAS: 487, Size 15.77: R164, N167, E134, S139, Q135, A131
SAS: 478, Size 13.78: R164, N167, E134, S130, Q135, A131
SAS: 477, Size 18.16: R143, N138, E134, S139, Q135, A131
SAS: 472, Size 22.70: R143, N115, E110, S139, Q135, A131
Epi#28
SAS: 554, Size 22.17: A101, Q107, I102, E134, Q135, S139, R143
SAS: 532, Size 19.36: 1102, Q107, W111, E110, Q135, S139, R143
SAS: 527, Size 22.79: V50, Q107, I102, E134, Q135, S139, R143
SAS: 509, Size 24.76: 1102, Q107, F49, E53, Q57, G46, R44
SAS: 508, Size 22.17: A101, Q107, W111, E110, Q135, S139, R143
Epi#31
SAS: 355, Size 21.56: L256, R180, N178, R10, W6, V197, D175
SAS: 352, Size 17.71: L251, R180, N178, R10, W6, V197, D175
Epi#34
SAS: 457, Size 18.37: V238, W235, S236, G144, R143, S139, S142
SAS: 457, Size 18.37: V238, W235, S236, G144, R143, S142, S139
SAS: 447, Size 14.02: V238, W235, S236, G144, R143, S142, T141
SAS: 447, Size 14.02: V238, W235, S236, G144, R143, T141, S142
SAS: 374, Size 18.37: V238, W235, S236, G144, R143, T141, S139
Epi#37
SAS: 397, Size 23.08: T254, A188, L256, R180, N177
SAS: 361, Size 18.08: T254, A188, L256, R180, N179
SAS: 328, Size 19.98: T254, A188, L256, R180, N178
Epi#39
SAS: 425, Size 16.36: A131, E134, R164, P127, G125, L124
SAS: 423, Size 16.02: A166, E134, R164, P127, G125, L124
SAS: 399, Size 16.36: A131, E134, R164, P129, G125, L124
SAS: 397, Size 16.02: A166, E134, R164, P129, G125, L124
SAS: 379, Size 16.36: A131, E134, T132, P129, G125, L124
Epi#40
SAS: 354, Size 15.77: A166, G189, T254, A188, S158
SAS: 351, Size 15.77: A166, G189, Y186, A188, T254
SAS: 334, Size 17.81: G189, A188, Y186, A156, S182
SAS: 334, Size 17.81: A188, G189, Y186, A156, S182
SAS: 330, Size 14.42: A166, G189, Y186, A188, S158
Epi#41
SAS: 217, Size 19.46: P204, Y208, L211, V197, S210
Epi#44
SAS: 407, Size 15.10: L256, R180, Y186, S158, A188, T254
SAS: 404, Size 18.45: L251, R180, Y186, S158, A188, T254
SAS: 387, Size 15.52: L256, R180, Y186, A188, G189, T254
SAS: 384, Size 16.23: L251, R180, Y186, A188, G189, T254
SAS: 373, Size 22.26: V197, R180, Y186, S158, A188, T254
Epi#46
SAS: 545, Size 12.69: A15, R269, R19, P14, N18, G20
Epi#53
SAS: 306, Size 18.06: W235, S234, Q239, K245
SAS: 277, Size 9.52: W235, S234, Q239, K229
SAS: 276, Size 9.46: W235, S234, Q230, K231
SAS: 268, Size 9.46: W235, P233, Q230, K231
SAS: 258, Size 14.50: W235, S236, Q239, K245
ProteaseE:
Epi#05
SAS: 461, Size 15.49: G189, A166, R164, P127, G125, S128
SAS: 459, Size 15.90: G189, A188, R164, P127, G125, S128
SAS: 435, Size 15.49: G189, A166, R164, P127, G125, S126
SAS: 433, Size 15.49: G189, A166, R164, P129, G125, S128
SAS: 433, Size 15.86: G189, A188, R164, P127, G125, S126
Epi#06
SAS: 518, Size 14.10: G189, A188, D157, S158, R164, P127
SAS: 490, Size 15.98: G189, A188, D157, S158, R164, P129
SAS: 460, Size 14.60: G155, A156, D157, S158, R164, P127
SAS: 432, Size 17.71: G155, A156, D157, S158, R164, P129
Epi#09
SAS: 482, Size 15.78: T254, G189, A166, R164, A188, S158
SAS: 311, Size 15.91: T22, G20, L21, R19, A15, S9
Epi#10
SAS: 455, Size 17.26: D175, N177, N179, S182, F183, G155, R180
SAS: 406, Size 13.76: D175, N212, N153, S182, F183, G155, R180
SAS: 383, Size 16.16: D175, N178, N179, S182, F183, G155, R180
SAS: 381, Size 15.82: D175, N212, N153, S154, F183, G155, R180
SAS: 347, Size 16.78: D175, N212, N153, A156, F183, G155, R180
Epi#12
SAS: 310, Size 13.48: P127, Y161, E134, P129
SAS: 306, Size 9.40: R164, Y161, E134, P129
SAS: 297, Size 9.40: R164, Y165, E134, P129
SAS: 285, Size 16.90: P127, Y161, E134, N167
SAS: 281, Size 18.68: P127, Y161, E134, N138
Epi#16
SAS: 673, Size 19.67: R164, P127, Y161, G125, S126, S154, D157,
A188, N255
SAS: 664, Size 20.60: R164, P129, Y165, G189, S158, S154, D157,
A188, N255
SAS: 645, Size 20.60: R164, P129, Y161, G125, S126, S154, D157,
A188, N255
SAS: 636, Size 14.89: R164, P127, Y161, G125, S126, S154, D157,
A156, N153
SAS: 627, Size 17.25: R164, P129, Y165, G189, S158, S154, D157,
A156, N153
Epi#17
SAS: 305, Size 15.86: A188, S158, R164, S126
SAS: 270, Size 12.73: A156, S158, R164, S126
Epi#18
SAS: 590, Size 17.32: S250, K246, S259, L256, A188, T254, L251
SAS: 551, Size 16.26: S259, K246, S250, L251, G252, T254, L256
SAS: 551, Size 16.26: S250, K246, S259, L251, G252, T254, L256
SAS: 551, Size 16.26: S250, K246, S259, L256, G252, T254, L251
SAS: 518, Size 16.26: S250, K246, S259, L251, G252, S253, L256
Epi#23
SAS: 471, Size 19.86: R143, N114, E110, S139, Q135, A131
SAS: 467, Size 13.75: R164, N167, E134, S130, Q135, A131
SAS: 467, Size 15.76: R164, N167, E134, S139, Q135, A131
SAS: 451, Size 22.69: R143, N115, E110, S139, Q135, A131
SAS: 446, Size 19.99: R143, N138, E134, S130, Q135, A131
Epi#28
SAS: 505, Size 19.43: 1102, Q107, W111, E110, Q135, S139, R143
SAS: 500, Size 22.22: A101, Q107, W111, E110, Q135, S139, R143
SAS: 499, Size 24.79: 1102, Q107, F49, E53, Q57, G46, R44
SAS: 494, Size 24.56: A101, Q107, F49, E53, Q57, G46, R44
SAS: 441, Size 24.79: 1102, Q107, E110, W111, F49, G46, R44
Epi#29
SAS: 216, Size 9.94: 143, R44, L41, E87
SAS: 209, Size 10.85: L73, N42, L41, E87
SAS: 200, Size 13.98: G46, R44, L41, E87
SAS: 199, Size 11.98: G45, R44, L41, E87
SAS: 197, Size 19.08: 177, N74, L41, E87
Epi#30
SAS: 318, Size 24.25: G20, L21, A15, H17, S85, L73, P39
SAS: 277, Size 24.25: G20, L21, A15, H17, S85, L41, P39
SAS: 258, Size 21.05: G20, L21, A15, H17, S85, L73, L41
Epi#31
SAS: 377, Size 21.62: L256, R180, N178, R10, W6, V197, D175
SAS: 370, Size 17.72: L251, R180, N178, R10, W6, V197, D175
Epi#33
SAS: 388, Size 15.92: Q135, Y165, P129, S126, R164
Epi#34
SAS: 420, Size 18.35: V238, W235, S236, G144, R143, S139, S142
SAS: 411, Size 13.98: V238, W235, S236, G144, R143, S142, T141
SAS: 341, Size 18.35: V238, W235, S236, G144, R143, S139, T141
Epi#37
SAS: 412, Size 23.05: T254, A188, L256, R180, N177
SAS: 378, Size 18.07: T254, A188, L256, R180, N179
SAS: 340, Size 20.00: T254, A188, L256, R180, N178
Epi#39
SAS: 445, Size 16.04: A166, E134, R164, P127, G125, L124
SAS: 432, Size 16.40: A131, E134, R164, P127, G125, L124
SAS: 417, Size 16.04: A166, E134, R164, P129, G125, L124
SAS: 404, Size 16.40: A131, E134, R164, P129, G125, L124
SAS: 376, Size 16.04: A166, E134, T132, P129, G125, L124
Epi#40
SAS: 374, Size 15.78: A166, G189, T254, A188, S158
SAS: 334, Size 15.78: A166, G189, Y186, A188, T254
SAS: 317, Size 11.62: A96, G59, T56, P54, S55
SAS: 312, Size 15.30: G98, G59, T56, P54, S55
SAS: 307, Size 15.49: G189, A166, Y165, P129, S128
Epi#41
SAS: 234, Size 19.50: P204, Y208, L211, V197, S210
SAS: 189, Size 19.50: P204, Y208, L211, V197, S215
Epi#42
SAS: 549, Size 16.42: L21, P14, S9, Q12, H17, R19, R269
Epi#44
SAS: 398, Size 15.10: L256, R180, Y186, S158, A188, T254
SAS: 391, Size 18.47: L251, R180, Y186, S158, A188, T254
SAS: 372, Size 15.51: L256, R180, Y186, A188, G189, T254
SAS: 371, Size 12.26: L256, R180, Y257, S250, G252, T254
SAS: 367, Size 15.51: L256, R180, Y186, S158, G189, T254
Epi#46
SAS: 575, Size 12.75: A15, R269, R19, P14, N18, G20
Epi#47
SAS: 491, Size 19.28: G45, E87, I43, R44, L41, N42, P39, S206
Epi#53
SAS: 202, Size 9.12: W235, P233, K231
SAS: 199, Size 9.12: W235, S234, K231
SAS: 182, Size 6.73: W235, P233, K229
SAS: 179, Size 7.76: W235, S234, K229
SAS: 131, Size 8.39: W235, S236, K229
Properase:
Epi#05
SAS: 456, Size 15.94: G189, A188, R164, P127, G125, S128
SAS: 453, Size 15.52: G189, A166, R164, P127, G125, S128
SAS: 451, Size 15.94: G157, A188, R164, P127, G125, S128
SAS: 427, Size 15.94: G189, A188, R164, P129, G125, S128
SAS: 424, Size 15.52: G189, A166, R164, P129, G125, S128
Epi#09
SAS: 480, Size 15.73: T254, G189, A166, R164, A188, S158
SAS: 302, Size 15.88: T22, G20, L21, R19, A15, S9
Epi#10
SAS: 470, Size 17.27: D175, N177, N179, S182, F183, G155, R180
SAS: 446, Size 13.75: D175, N212, N153, S182, F183, G155, R180
SAS: 420, Size 15.84: D175, N212, N153, S154, F183, G155, R180
SAS: 396, Size 16.09: D175, N178, N179, S182, F183, G155, R180
SAS: 380, Size 16.78: D175, N212, N153, A156, F183, G155, R180
Epi#12
SAS: 296, Size 9.36: R164, Y161, E134, P129
SAS: 295, Size 13.45: P127, Y161, E134, P129
SAS: 291, Size 9.36: R164, Y165, E134, P129
SAS: 271, Size 14.70: R164, Y161, E134, N102
SAS: 270, Size 13.45: P127, Y161, E134, N102
Epi#17
SAS: 283, Size 15.87: A188, S158, R164, S126
SAS: 241, Size 12.73: A156, S158, R164, S126
Epi#18
SAS: 474, Size 16.26: S250, K245, S259, L256, A188, T254, L251
SAS: 435, Size 14.14: S250, K245, S259, L256, G252, T254, L251
SAS: 398, Size 14.14: S259, K245, S250, L251, G252, S253, L256
Epi#19
SAS: 260, Size 21.26: E110, T141, S236, Q239, R241
Epi#23
SAS: 491, Size 19.86: R143, N114, E110, S139, Q135, A131
SAS: 482, Size 15.76: R164, N167, E134, S139, Q135, A131
SAS: 465, Size 22.69: R143, N115, E110, S139, Q135, A131
SAS: 462, Size 18.17: R143, N138, E134, S139, Q135, A131
SAS: 439, Size 18.17: R143, N138, E110, S139, Q135, A131
Epi#28
SAS: 445, Size 22.79: V50, Q107, W111, E110, Q135, S139, R143
SAS: 426, Size 19.06: V50, Q107, F49, E53, Q57, G46, R44
SAS: 370, Size 19.06: V50, Q107, E110, W111, F49, G46, R44
Epi#31
SAS: 347, Size 21.62: L256, R180, N178, R10, W6, V197, D175
SAS: 339, Size 17.74: L251, R180, N178, R10, W6, V197, D175
Epi#33
SAS: 368, Size 15.95: Q135, Y165, P129, S126, R164
Epi#34
SAS: 445, Size 18.39: V238, W235, S236, G144, R143, S139, S142
SAS: 436, Size 14.07: V238, W235, S236, G144, R143, S142, T141
SAS: 358, Size 18.39: V238, W235, S236, G144, R143, T141, S139
Epi#37
SAS: 415, Size 23.03: T254, A188, L256, R180, N177
SAS: 374, Size 18.04: T254, A188, L256, R180, N179
SAS: 341, Size 19.93: T254, A188, L256, R180, N178
Epi#39
SAS: 323, Size 11.55: A15, E265, H17, R19, P14, G20, L21
SAS: 238, Size 12.13: A15, E265, H17, T22, P14, G20, L21
Epi#40
SAS: 370, Size 15.73: A166, G189, T254, A188, S158
SAS: 360, Size 15.73: A166, G189, Y186, A188, T254
SAS: 324, Size 17.80: A188, G189, Y186, A156, S182
SAS: 321, Size 23.71: A166, G189, Y186, A156, S182
Epi#41
SAS: 228, Size 19.53: P204, Y208, L211, V197, S210
Epi#42
SAS: 554, Size 16.31: L21, P14, S9, Q12, H17, R19, R269
Epi#44
SAS: 406, Size 15.06: L256, R180, Y186, S158, A188, T254
SAS: 398, Size 18.38: L251, R180, Y186, S158, A188, T254
SAS: 395, Size 12.22: L256, R180, Y257, S250, G252, T254
SAS: 392, Size 15.49: L256, R180, Y186, A188, G189, T254
SAS: 387, Size 12.22: L251, R180, Y257, S250, G252, T254
Epi#46
SAS: 581, Size 12.65: A15, R269, R19, P14, N18, G20
Epi#53
SAS: 297, Size 18.06: W235, S234, Q239, K245
SAS: 283, Size 9.54: W235, S234, Q239, K229
SAS: 250, Size 9.46: W235, S234, Q230, K231
SAS: 249, Size 14.49: W235, S236, Q239, K245
SAS: 247, Size 9.46: W235, P233, Q230, K231
Release:
Epi#05
SAS: 461, Size 17.25: G158, A189, R165, P128, G126, S129
SAS: 439, Size 17.22: G158, A189, R165, P128, G126, S127
SAS: 436, Size 17.25: G158, A189, S159, P128, G126, S129
SAS: 420, Size 17.25: G158, A189, R165, P130, G126, S129
SAS: 414, Size 17.22: G158, A189, S159, P128, G126, S127
Epi#09
SAS: 510, Size 22.37: T22, G20, R19, A15, R270, A267, T250
SAS: 501, Size 22.37: L21, G20, R19, A15, R270, A267, T250
Epi#10
SAS: 458, Size 17.50: D176, N178, N180, S183, F184, G156, R181
SAS: 424, Size 13.68: D176, N213, N154, S183, F184, G156, R181
SAS: 407, Size 15.87: D176, N213, N154, S155, F184, G156, R181
SAS: 392, Size 16.18: D176, N179, N180, S183, F184, G156, R181
SAS: 362, Size 16.73: D176, N213, N154, A157, F184, G156, R181
Epi#12
SAS: 323, Size 9.38: R45, Y90, E88, N43
SAS: 312, Size 13.53: P128, Y162, E135, P130
SAS: 302, Size 9.46: R165, Y162, E135, P130
SAS: 296, Size 9.46: R165, Y166, E135, P130
SAS: 295, Size 13.19: T255, Y187, E190, S159
Epi#18
SAS: 431, Size 15.20: S251, K246, S260, L257, A189, T255, L252
SAS: 398, Size 14.35: S251, K246, S260, L252, G253, T255, L257
SAS: 378, Size 14.35: S251, K246, S260, L257, G253, T250, L252
Epi#19
SAS: 285, Size 21.53: E111, T142, S237, Q240, R242
SAS: 275, Size 12.58: D119, T142, S237, Q240, R242
Epi#23
SAS: 512, Size 22.29: R45, N43, E88, S24, Q231, P234
SAS: 476, Size 19.71: R144, N115, E111, S140, Q136, A132
SAS: 460, Size 13.83: R165, N168, E135, S131, Q136, A132
SAS: 455, Size 20.11: R144, N139, E135, S131, Q136, A132
SAS: 452, Size 15.83: R165, N168, E135, S140, Q136, A132
Epi#25
SAS: 293, Size 13.93: R45, K27, D119, E88
Epi#28
SAS: 502, Size 19.99: V103, Q108, W112, E111, Q136, S140, R144
SAS: 476, Size 21.74: V51, Q108, F50, E54, Q58, S37, R45
SAS: 472, Size 24.93: V103, Q108, F50, E54, Q58, G47, R45
SAS: 469, Size 23.18: V51, Q108, W112, E111, Q136, S140, R144
SAS: 439, Size 19.16: V51, Q108, F50, E54, Q58, G47, R45
Epi#31
SAS: 354, Size 21.73: L257, R181, N179, R10, W6, V198, D176
SAS: 348, Size 17.85: L252, R181, N179, R10, W6, V198, D176
Epi#33
SAS: 396, Size 22.75: Q201, Y204, P205, S37, R45
SAS: 379, Size 22.75: Q201, Y209, P205, S37, R45
SAS: 357, Size 18.39: H63, Y204, P205, S37, R45
Epi#34
SAS: 466, Size 13.97: V239, W236, S237, G145, R144, S143, T142
SAS: 463, Size 18.37: V239, W236, S237, G145, R144, S140, S143
SAS: 387, Size 18.37: V239, W236, S237, G145, R144, S140, T142
Epi#36
SAS: 206, Size 22.37: T250, A267, A15, G20, T22
Epi#37
SAS: 400, Size 22.59: T255, A189, L257, R181, N178
SAS: 359, Size 17.59: T255, A189, L257, R181, N180
SAS: 334, Size 19.35: T255, A189, L257, R181, N179
Epi#39
SAS: 464, Size 16.36: A167, E135, R165, P128, G126, L125
SAS: 444, Size 16.52: A132, E135, R165, P128, G126, L125
SAS: 441, Size 16.36: A167, E190, R165, P128, G126, L125
SAS: 441, Size 18.98: A189, E190, R165, P128, G126, L125
SAS: 423, Size 16.36: A167, E135, R165, P130, G126, L125
Epi#40
SAS: 324, Size 11.66: A97, G60, T57, P55, S56
SAS: 316, Size 17.09: G158, A189, Y187, A157, S183
SAS: 307, Size 14.92: G158, A157, Y187, A189, T255
SAS: 307, Size 15.34: G99, G60, T57, P55, S56
Epi#41
SAS: 222, Size 19.74: P205, Y209, L212, V198, S211
Epi#42
SAS: 544, Size 16.22: L21, P14, S9, Q12, H17, R19, R270
Epi#44
SAS: 421, Size 14.87: L257, R181, Y187, S159, A189, T255
SAS: 415, Size 18.81: L252, R181, Y187, S159, A189, T255
SAS: 389, Size 22.36: V198, R181, Y187, S159, A189, T255
SAS: 389, Size 21.81:144, R45, Y90, A48, V51, P52
SAS: 386, Size 19.16:144, R45, Y90, A48, V51, P55
Epi#46
SAS: 557, Size 14.54: A267, R270, R19, P14, N18, G20
SAS: 553, Size 12.63: A15, R270, R19, P14, N18, G20
SAS: 540, Size 13.10: A267, R270, R19, P14, N18, A15
SAS: 444, Size 14.54: A267, R270, R19, P14, G20, A15
Epi#47
SAS: 627, Size 16.22: A267, R270, A15, R19, L21, N18, P14, S9
SAS: 436, Size 15.11: A267, E266, A15, R19, L21, N18, P14, S9
Epi#51
SAS: 545, Size 21.66: L21, R19, H17, D75, S77, I78, S3, W6
SAS: 485, Size 21.66: L21, R19, H17, D75, Q2, I78, S3, W6
Epi#53
SAS: 328, Size 9.43: W236, S235, Q231, K232
SAS: 316, Size 9.43: W236, P234, Q231, K232
SAS: 301, Size 18.21: W236, S235, Q240, K246
SAS: 246, Size 14.68: W236, S237, Q240, K246
[0598] "SAS" is solvent accessible surface. "Size" is the total
surface area of the epitope in A2.
Example 12
[0599] The object of this example is to provide evidence showing
that subtilisins with an homology to BPN' of as low as 44.8% reveal
a similar epitope distribution as BPN'.
[0600] Alcalase, Protease B, Savinase, Esperase, and PD498 (which
range from 44.8% to 69.5% in sequence identity to BPN') were
epitope mapped as described in the above example, and compared with
epitope mapped BPN' (FIG. 1).
[0601] The data in FIG. 1 show a significant overlap between the
areas on the primary structure of the respective proteases.
Overall, 6 regions were identified: 1-20, 35-65, 95-115, 130-145,
170-220, and 260-270.
[0602] Even better overlap between the epitope sequences can be
found among proteins of higher sequence identity, such as within
the Savinase-like subtilisins with more than 81% identity,
preferably more than 85%, more preferably more than 90%, even more
preferably more than 96% or most preferably more than 98%
identity.
Example 13
Wash Performance
[0603] The following example provides results from a number of
washing tests that were conducted under the conditions
indicated
TABLE-US-00020 TABLE 9 Experimental conditions for evaluation of
Subtilisin variants I44V. Detergent OMO Acao Detergent dose 2.5 g/l
PH 10.5 Wash time 14 min. Temperature 25.degree. C. Water hardness
9.degree. dH Enzymes Subtilisin variant I44V Enzyme conc. 10 nM
Test system 150 ml glass beakers with a stirring rod Textile/volume
5 textile pieces (O 2.5 cm) in 50 ml detergent Test material
EMPA117 from Center for Test materials, Holland
TABLE-US-00021 TABLE 10 Experimental conditions for evaluation of
Subtilisin variants Q12D. Detergent Persil Powder Detergent dose 4
g/l PH 10.5 Wash time 20 min. Temperature 30.degree. C. Water
hardness 18.degree. dH Enzymes Subtilisin variant Q12D Enzyme conc.
10 nM Test system 150 ml glass beakers with a stirring rod
Textile/volume 5 textile pieces (O 2.5 cm) in 50 ml detergent Test
material EMPA116 from Center for Test materials, Holland
TABLE-US-00022 TABLE 11 Experimental conditions for evaluation of
Subtilisin variants Q12D. Detergent Tide Detergent dose 1 g/l PH
10.5 Wash time 10 min. Temperature 25.degree. C. Water hardness
6.degree. dH Enzymes Subtilisin variant Q12D Enzyme conc. 10 nM
Test system 150 ml glass beakers with a stirring rod Textile/volume
5 textile pieces (O 2.5 cm) in 50 ml detergent Test material
EMPA117 from Center for Test materials, Holland
[0604] pH is adjusted to 10.5 which is within the normal range for
a powder detergent.
[0605] Water hardness was adjusted by adding CaCl.sub.2 and
MgCl.sub.2 (Ca.sup.2+:Mg.sup.2+=2:1) to deionized water (see also
Surfactants in Consumer Products--Theory, Technology and
Application, Springer Verlag 1986). pH of the detergent solution
was adjusted to pH 10.5 by addition of HCl.
[0606] Measurement of reflectance (R) on the test material was done
at 460 nm using a Macbeth ColorEye 7000 photometer. The
measurements were done according to the manufacturers protocol.
[0607] The wash performance of the variants was evaluated by
calculating a performance factor:
P=(R.sub.Variant-R.sub.Blank)/(R.sub.Savinase-R.sub.Blank)
P: Performance factor R.sub.Variant: Reflectance of test material
washed with variant R.sub.Savinase: Reflectance of test material
washed with Savinase.RTM. R.sub.Blank: Reflectance of test material
washed with no enzyme
[0608] The variants all have improved wash performance compared to
Savinase.RTM.--i.e. P>1.
[0609] The variants can be divided into improvement classes
designated with capital letters:
Class A: 1<P.ltoreq.1.5
Class B: 1.5<P.ltoreq.2
Class C: P>2
TABLE-US-00023 [0610] TABLE 12 Subtilisin variants and improvement
classes. Improvement class Variants C I44V, Q12D
[0611] As it can be seen from Table 12 SAVINASE.RTM. variants of
the invention exhibits an improvement in wash performance.
Sequence CWU 1
1
2481269PRTT. lanuginosus 1Glu Val Ser Gln Asp Leu Phe Asn Gln Phe
Asn Leu Phe Ala Gln Tyr1 5 10 15Ser Ala Ala Ala Tyr Cys Gly Lys Asn
Asn Asp Ala Pro Ala Gly Thr 20 25 30Asn Ile Thr Cys Thr Gly Asn Ala
Cys Pro Glu Val Glu Lys Ala Asp 35 40 45Ala Thr Phe Leu Tyr Ser Phe
Glu Asp Ser Gly Val Gly Asp Val Thr 50 55 60Gly Phe Leu Ala Leu Asp
Asn Thr Asn Lys Leu Ile Val Leu Ser Phe65 70 75 80Arg Gly Ser Arg
Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp 85 90 95Leu Lys Glu
Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly 100 105 110Phe
Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 115 120
125Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly
130 135 140His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp
Leu Arg145 150 155 160Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr
Gly Ala Pro Arg Val 165 170 175Gly Asn Arg Ala Phe Ala Glu Phe Leu
Thr Val Gln Thr Gly Gly Thr 180 185 190Leu Tyr Arg Ile Thr His Thr
Asn Asp Ile Val Pro Arg Leu Pro Pro 195 200 205Arg Glu Phe Gly Tyr
Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 210 215 220Gly Thr Leu
Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly225 230 235
240Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro
245 250 255Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 260
2652481PRTBacillus Halmapalus 2Thr Asn Gly Thr Met Met Gln Tyr Phe
Glu Trp His Leu Pro Asn Asp1 5 10 15Gly Asn His Trp Asn Arg Leu Arg
Asp Asp Ala Ser Asn Leu Arg Asn 20 25 30Arg Gly Ile Thr Ala Ile Trp
Ile Pro Pro Ala Trp Lys Gly Thr Ser 35 40 45Gln Asn Asp Val Gly Tyr
Gly Ala Tyr Asp Leu Tyr Asp Leu Gly Glu 50 55 60Phe Asn Gln Lys Gly
Thr Val Arg Thr Lys Tyr Gly Thr Arg Ser Gln65 70 75 80Leu Glu Ser
Ala Ile His Ala Leu Lys Asn Asn Gly Val Gln Val Tyr 85 90 95Gly Asp
Val Val Met Asn His Lys Gly Gly Ala Asp Ala Thr Glu Asn 100 105
110Val Leu Ala Val Glu Val Asn Pro Asn Asn Arg Asn Gln Glu Ile Ser
115 120 125Gly Asp Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp Phe Pro
Gly Arg 130 135 140Gly Asn Thr Tyr Ser Asp Phe Lys Trp Arg Trp Tyr
His Phe Asp Gly145 150 155 160Val Asp Trp Asp Gln Ser Arg Gln Phe
Gln Asn Arg Ile Tyr Lys Phe 165 170 175Arg Gly Asp Gly Lys Ala Trp
Asp Trp Glu Val Asp Ser Glu Asn Gly 180 185 190Asn Tyr Asp Tyr Leu
Met Tyr Ala Asp Val Asp Met Asp His Pro Glu 195 200 205Val Val Asn
Glu Leu Arg Arg Trp Gly Glu Trp Tyr Thr Asn Thr Leu 210 215 220Asn
Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile Lys Tyr Ser225 230
235 240Phe Thr Arg Asp Trp Leu Thr His Val Arg Asn Ala Thr Gly Lys
Glu 245 250 255Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Gly
Ala Leu Glu 260 265 270Asn Tyr Leu Asn Lys Thr Asn Trp Asn His Ser
Val Phe Asp Val Pro 275 280 285Leu His Tyr Asn Leu Tyr Asn Ala Ser
Asn Ser Gly Gly Asn Tyr Asp 290 295 300Met Ala Lys Leu Leu Asn Gly
Thr Val Val Gln Lys His Pro Met His305 310 315 320Ala Val Thr Phe
Val Asp Asn His Asp Ser Gln Pro Gly Glu Ser Leu 325 330 335Glu Ser
Phe Val Gln Glu Trp Phe Lys Pro Leu Ala Tyr Ala Leu Ile 340 345
350Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp Tyr Tyr
355 360 365Gly Ile Pro Thr His Ser Val Pro Ala Met Lys Ala Lys Ile
Asp Pro 370 375 380Ile Leu Glu Ala Arg Gln Asn Phe Ala Tyr Gly Thr
Gln His Asp Tyr385 390 395 400Phe Asp His His Asn Ile Ile Gly Trp
Thr Arg Glu Gly Asn Thr Thr 405 410 415His Pro Asn Ser Gly Leu Ala
Thr Ile Met Ser Asp Gly Pro Gly Gly 420 425 430Glu Lys Trp Met Tyr
Val Gly Gln Asn Lys Ala Gly Gln Val Trp His 435 440 445Asp Ile Thr
Gly Asn Lys Pro Gly Thr Val Thr Ile Asn Ala Asp Gly 450 455 460Trp
Ala Asn Phe Ser Val Asn Gly Gly Ser Val Ser Ile Trp Val Lys465 470
475 480Arg3504PRTCoprinus cinereus 3Gln Ile Val Asn Ser Val Asp Thr
Met Thr Leu Thr Asn Ala Asn Val1 5 10 15Ser Pro Asp Gly Phe Thr Arg
Ala Gly Ile Leu Val Asn Gly Val His 20 25 30Gly Pro Leu Ile Arg Gly
Gly Lys Asn Asp Asn Phe Glu Leu Asn Val 35 40 45Val Asn Asp Leu Asp
Asn Pro Thr Met Leu Arg Pro Thr Ser Ile His 50 55 60Trp His Gly Leu
Phe Gln Arg Gly Thr Asn Trp Ala Asn Gly Ala Asp65 70 75 80Gly Val
Asn Gln Cys Pro Ile Ser Pro Gly His Ala Phe Leu Tyr Lys 85 90 95Phe
Thr Pro Ala Gly His Ala Gly Thr Phe Trp Tyr His Ser His Phe 100 105
110Gly Thr Gln Tyr Cys Asp Gly Leu Arg Gly Pro Met Val Ile Tyr Asp
115 120 125Asp Asn Asp Pro His Ala Ala Leu Tyr Asp Glu Asp Asp Glu
Asn Thr 130 135 140Ile Ile Thr Leu Ala Asp Trp Tyr His Ile Pro Ala
Pro Ser Ile Gln145 150 155 160Gly Ala Ala Gln Pro Asp Ala Thr Leu
Ile Asn Gly Lys Gly Arg Tyr 165 170 175Val Gly Gly Pro Ala Ala Glu
Leu Ser Ile Val Asn Val Glu Gln Gly 180 185 190Lys Lys Tyr Arg Met
Arg Leu Ile Ser Leu Ser Cys Asp Pro Asn Trp 195 200 205Gln Phe Ser
Ile Asp Gly His Glu Leu Thr Ile Ile Glu Val Asp Gly 210 215 220Asn
Leu Thr Glu Pro His Thr Val Asp Arg Leu Gln Ile Phe Thr Gly225 230
235 240Gln Arg Tyr Ser Phe Val Leu Asp Ala Asn Gln Pro Val Asp Asn
Tyr 245 250 255Trp Ile Arg Ala Gln Pro Asn Lys Gly Arg Asn Gly Leu
Ala Gly Thr 260 265 270Phe Ala Asn Gly Val Asn Ser Ala Ile Leu Arg
Tyr Ala Gly Ala Ala 275 280 285Asn Ala Asp Pro Thr Thr Ser Ala Asn
Pro Asn Pro Ala Gln Leu Asn 290 295 300Glu Ala Asp Leu His Ala Leu
Ile Asp Pro Ala Ala Pro Gly Ile Pro305 310 315 320Thr Pro Gly Ala
Ala Asn Val Asn Leu Arg Phe Gln Leu Gly Phe Ser 325 330 335Gly Gly
Arg Phe Thr Ile Asn Gly Thr Ala Tyr Glu Ser Pro Ser Val 340 345
350Pro Thr Leu Leu Gln Ile Met Ser Gly Ala Gln Ser Ala Asn Asp Leu
355 360 365Leu Pro Ala Gly Ser Val Tyr Glu Leu Pro Arg Asn Gln Val
Val Glu 370 375 380Leu Val Val Pro Ala Gly Val Leu Gly Gly Pro His
Pro Phe His Leu385 390 395 400His Gly His Ala Phe Ser Val Val Arg
Ser Ala Gly Ser Ser Thr Tyr 405 410 415Asn Phe Val Asn Pro Val Lys
Arg Asp Val Val Ser Leu Gly Val Thr 420 425 430Gly Asp Glu Val Thr
Ile Arg Phe Val Thr Asp Asn Pro Gly Pro Trp 435 440 445Phe Phe His
Cys His Ile Glu Phe His Leu Met Asn Gly Leu Ala Ile 450 455 460Val
Phe Ala Glu Asp Met Ala Asn Thr Val Asp Ala Asn Asn Pro Pro465 470
475 480Val Glu Trp Ala Gln Leu Cys Glu Ile Tyr Asp Asp Leu Pro Pro
Glu 485 490 495Ala Thr Ser Ile Gln Thr Val Val 5004213PRTHumicola
insolens 4Ala Asp Gly Arg Ser Thr Arg Tyr Trp Asp Cys Cys Lys Pro
Ser Cys1 5 10 15Gly Trp Ala Lys Lys Ala Pro Val Asn Gln Pro Val Phe
Ser Cys Asn 20 25 30Ala Asn Phe Gln Arg Ile Thr Asp Phe Asp Ala Lys
Ser Gly Cys Glu 35 40 45Pro Gly Gly Val Ala Tyr Ser Cys Ala Asp Gln
Thr Pro Trp Ala Val 50 55 60Asn Asp Asp Phe Ala Leu Gly Phe Ala Ala
Thr Ser Ile Ala Gly Ser65 70 75 80Asn Glu Ala Gly Trp Cys Cys Ala
Cys Tyr Glu Leu Thr Phe Thr Ser 85 90 95Gly Pro Val Ala Gly Lys Lys
Met Val Val Gln Ser Thr Ser Thr Gly 100 105 110Gly Asp Leu Gly Ser
Asn His Phe Asp Leu Asn Ile Pro Gly Gly Gly 115 120 125Val Gly Ile
Phe Asp Gly Cys Thr Pro Gln Phe Gly Gly Leu Pro Gly 130 135 140Gln
Arg Tyr Gly Gly Ile Ser Ser Arg Asn Glu Cys Asp Arg Phe Pro145 150
155 160Asp Ala Leu Lys Pro Gly Cys Tyr Trp Arg Phe Asp Trp Phe Lys
Asn 165 170 175Ala Asp Asn Pro Ser Phe Ser Phe Arg Gln Val Gln Cys
Pro Ala Glu 180 185 190Leu Val Ala Arg Thr Gly Cys Arg Arg Asn Asp
Asp Gly Asn Phe Pro 195 200 205Ala Val Gln Ile Pro
2105305PRTHumicola insolens 5Met Arg Ser Ser Pro Leu Leu Pro Ser
Ala Val Val Ala Ala Leu Pro1 5 10 15Val Leu Ala Leu Ala Ala Asp Gly
Arg Ser Thr Arg Tyr Trp Asp Cys 20 25 30Cys Lys Pro Ser Cys Gly Trp
Ala Lys Lys Ala Pro Val Asn Gln Pro 35 40 45Val Phe Ser Cys Asn Ala
Asn Phe Gln Arg Ile Thr Asp Phe Asp Ala 50 55 60Lys Ser Gly Cys Glu
Pro Gly Gly Val Ala Tyr Ser Cys Ala Asp Gln65 70 75 80Thr Pro Trp
Ala Val Asn Asp Asp Phe Ala Leu Gly Phe Ala Ala Thr 85 90 95Ser Ile
Ala Gly Ser Asn Glu Ala Gly Trp Cys Cys Ala Cys Tyr Glu 100 105
110Leu Thr Phe Thr Ser Gly Pro Val Ala Gly Lys Lys Met Val Val Gln
115 120 125Ser Thr Ser Thr Gly Gly Asp Leu Gly Ser Asn His Phe Asp
Leu Asn 130 135 140Ile Pro Gly Gly Gly Val Gly Ile Phe Asp Gly Cys
Thr Pro Gln Phe145 150 155 160Gly Gly Leu Pro Gly Gln Arg Tyr Gly
Gly Ile Ser Ser Arg Asn Glu 165 170 175Cys Asp Arg Phe Pro Asp Ala
Leu Lys Pro Gly Cys Tyr Trp Arg Phe 180 185 190Asp Trp Phe Lys Asn
Ala Asp Asn Pro Ser Phe Ser Phe Arg Gln Val 195 200 205Gln Cys Pro
Ala Glu Leu Val Ala Arg Thr Gly Cys Arg Arg Asn Asp 210 215 220Asp
Gly Asn Phe Pro Ala Val Gln Ile Pro Ser Ser Ser Thr Ser Ser225 230
235 240Pro Val Asn Gln Pro Thr Ser Thr Ser Thr Thr Ser Thr Ser Thr
Thr 245 250 255Ser Ser Pro Pro Val Gln Pro Thr Thr Pro Ser Gly Cys
Thr Ala Glu 260 265 270Arg Trp Ala Gln Cys Gly Gly Asn Gly Trp Ser
Gly Cys Thr Thr Cys 275 280 285Val Ala Gly Ser Thr Cys Thr Lys Ile
Asn Asp Trp Tyr His Gln Cys 290 295 300Leu3056159PRTBetula pendula
6Gly Val Phe Asn Tyr Glu Thr Glu Thr Thr Ser Val Ile Pro Ala Ala1 5
10 15Arg Leu Phe Lys Ala Phe Ile Leu Asp Gly Asp Asn Leu Phe Pro
Lys 20 25 30Val Ala Pro Gln Ala Ile Ser Ser Val Glu Asn Ile Glu Gly
Asn Gly 35 40 45Gly Pro Gly Thr Ile Lys Lys Ile Ser Phe Pro Glu Gly
Phe Pro Phe 50 55 60Lys Tyr Val Lys Asp Arg Val Asp Glu Val Asp His
Thr Asn Phe Lys65 70 75 80Tyr Asn Tyr Ser Val Ile Glu Gly Gly Pro
Ile Gly Asp Thr Leu Glu 85 90 95Lys Ile Ser Asn Glu Ile Lys Ile Val
Ala Thr Pro Asp Gly Gly Ser 100 105 110Ile Leu Lys Ile Ser Asn Lys
Tyr His Thr Lys Gly Asp His Glu Val 115 120 125Lys Ala Glu Gln Val
Lys Ala Ser Lys Glu Met Gly Glu Thr Leu Leu 130 135 140Arg Ala Val
Glu Ser Tyr Leu Leu Ala His Ser Asp Ala Tyr Asn145 150
1557129PRTDermatophagoides farinae 7Asp Gln Val Asp Val Lys Asp Cys
Ala Asn Asn Glu Ile Lys Lys Val1 5 10 15Met Val Asp Gly Cys His Gly
Ser Asp Pro Cys Ile Ile His Arg Gly 20 25 30Lys Pro Phe Thr Leu Glu
Ala Leu Phe Asp Ala Asn Gln Asn Thr Lys 35 40 45Thr Ala Lys Ile Glu
Ile Lys Ala Ser Leu Asp Gly Leu Glu Ile Asp 50 55 60Val Pro Gly Ile
Asp Thr Asn Ala Cys His Phe Val Lys Cys Pro Leu65 70 75 80Val Lys
Gly Gln Gln Tyr Asp Ile Lys Tyr Thr Trp Asn Val Pro Lys 85 90 95Ile
Ala Pro Lys Ser Glu Asn Val Val Val Thr Val Lys Leu Ile Gly 100 105
110Asp Asn Gly Val Leu Ala Cys Ala Ile Ala Thr His Gly Lys Ile Arg
115 120 125Asp8129PRTDermatophagoides pteronyssinus 8Ser Gln Val
Asp Val Lys Asp Cys Ala Asn His Glu Ile Lys Lys Val1 5 10 15Leu Val
Pro Gly Cys His Gly Ser Glu Pro Cys Ile Ile His Arg Gly 20 25 30Lys
Pro Phe Gln Leu Glu Ala Val Phe Glu Ala Asn Gln Asn Thr Lys 35 40
45Thr Ala Lys Ile Glu Ile Lys Ala Ser Ile Asp Gly Leu Glu Val Asp
50 55 60Val Pro Gly Ile Asp Pro Asn Ala Cys His Tyr Met Lys Cys Pro
Leu65 70 75 80Val Lys Gly Gln Gln Tyr Asp Ile Lys Tyr Thr Trp Asn
Val Pro Lys 85 90 95Ile Ala Pro Lys Ser Glu Asn Val Val Val Thr Val
Lys Val Met Gly 100 105 110Asp Asp Gly Val Leu Ala Cys Ala Ile Ala
Thr His Ala Lys Ile Arg 115 120 125Asp994PRTPhleum pratense 9Val
Pro Lys Val Thr Phe Thr Val Glu Lys Gly Ser Asn Glu Lys His1 5 10
15Leu Ala Val Leu Val Lys Tyr Glu Gly Asp Thr Met Ala Glu Val Glu
20 25 30Leu Arg Glu His Gly Ser Asp Glu Trp Val Ala Met Thr Lys Gly
Glu 35 40 45Gly Gly Val Trp Thr Phe Asp Ser Glu Glu Pro Leu Gln Gly
Pro Phe 50 55 60Asn Phe Arg Phe Leu Thr Glu Lys Gly Met Lys Asn Val
Phe Asp Asp65 70 75 80Val Val Pro Glu Lys Tyr Thr Ile Gly Ala Thr
Tyr Ala Pro 85 9010338PRTBacillus subtilis 10Ala Gln Ser Val Pro
Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu1 5 10 15His Ser Gln Gly
Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 20 25 30Ser Gly Ile
Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 35 40 45Ser Met
Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 50 55 60Gly
Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly65 70 75
80Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu
85 90 95Gly Ala Asp Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile
Glu 100 105 110Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Met Ser
Leu Gly Gly 115 120 125Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val
Asp Lys Ala Val Ala 130 135 140Ser Gly Val Val Val Val Ala Ala Ala
Gly Asn Glu Gly Thr Ser Gly145 150 155 160Ser Ser Ser Thr Val Gly
Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 165
170 175Val Gly Ala Val Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser
Val 180 185 190Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile
Gln Ser Thr 195 200 205Leu Pro Gly Asn Lys Tyr Gly Ala Tyr Asn Gly
Thr Ser Met Ala Ser 210 215 220Pro His Val Ala Gly Ala Ala Ala Leu
Ile Leu Ser Lys His Pro Asn225 230 235 240Trp Thr Asn Thr Gln Val
Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 245 250 255Leu Gly Asp Ser
Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 260 265 270Ala Ala
Gln Lys Ser Phe Pro Glu Val Val Gly Lys Thr Val Asp Gln 275 280
285Ala Arg Glu Tyr Phe Thr Leu His Tyr Pro Gln Tyr Asp Val Tyr Phe
290 295 300Leu Pro Glu Gly Ser Pro Val Thr Leu Asp Leu Arg Tyr Asn
Arg Val305 310 315 320Lys Val Phe Tyr Asn Pro Gly Thr Asn Val Val
Asn His Val Pro His 325 330 335Val Gly11268PRTBacillus lentus 11Gln
Thr Val Pro Trp Gly Ile Ser Phe Ile Asn Thr Gln Gln Ala His1 5 10
15Asn Arg Gly Ile Phe Gly Asn Gly Ala Arg Val Ala Val Leu Asp Thr
20 25 30Gly Ile Ala Ser His Pro Asp Leu Arg Ile Ala Gly Gly Ala Ser
Phe 35 40 45Ile Ser Ser Glu Pro Ser Tyr His Asp Asn Asn Gly His Gly
Thr His 50 55 60Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly
Val Leu Gly65 70 75 80Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys
Val Leu Asp Arg Asn 85 90 95Gly Ser Gly Ser Leu Ala Ser Val Ala Gln
Gly Ile Glu Trp Ala Ile 100 105 110Asn Asn Asn Met His Ile Ile Asn
Met Ser Leu Gly Ser Thr Ser Gly 115 120 125Ser Ser Thr Leu Glu Leu
Ala Val Asn Arg Ala Asn Asn Ala Gly Ile 130 135 140Leu Leu Val Gly
Ala Ala Gly Asn Thr Gly Arg Gln Gly Val Asn Tyr145 150 155 160Pro
Ala Arg Tyr Ser Gly Val Met Ala Val Ala Ala Val Asp Gln Asn 165 170
175Gly Gln Arg Ala Ser Phe Ser Thr Tyr Gly Pro Glu Ile Glu Ile Ser
180 185 190Ala Pro Gly Val Asn Val Asn Ser Thr Tyr Thr Gly Asn Arg
Tyr Val 195 200 205Ser Leu Ser Gly Thr Ser Met Ala Thr Pro His Val
Ala Gly Val Ala 210 215 220Ala Leu Val Lys Ser Arg Tyr Pro Ser Tyr
Thr Asn Asn Gln Ile Arg225 230 235 240Gln Arg Ile Asn Gln Thr Ala
Thr Tyr Leu Gly Ser Pro Ser Leu Tyr 245 250 255Gly Asn Gly Leu Val
His Ala Gly Arg Ala Thr Gln 260 26512150PRTBos taurus 12Ile Asp Pro
Ser Lys Ile Pro Gly Glu Trp Arg Ile Ile Tyr Ala Ala1 5 10 15Ala Asp
Asn Lys Asp Lys Ile Val Glu Gly Gly Pro Leu Arg Asn Tyr 20 25 30Tyr
Arg Arg Ile Glu Cys Ile Asn Asp Cys Glu Ser Leu Ser Ile Thr 35 40
45Phe Tyr Leu Lys Asp Gln Gly Thr Cys Leu Leu Leu Thr Glu Val Ala
50 55 60Lys Arg Gln Glu Gly Tyr Val Tyr Val Leu Glu Phe Tyr Gly Thr
Asn65 70 75 80Thr Leu Glu Val Ile His Val Ser Glu Asn Met Leu Val
Thr Tyr Val 85 90 95Glu Asn Tyr Asp Gly Glu Arg Ile Thr Lys Met Thr
Glu Gly Leu Ala 100 105 110Lys Gly Thr Ser Phe Thr Pro Glu Glu Leu
Glu Lys Tyr Gln Gln Leu 115 120 125Asn Ser Glu Arg Gly Val Pro Asn
Glu Asn Ile Glu Asn Leu Ile Lys 130 135 140Thr Asp Asn Cys Pro
Pro145 15013159PRTEquus caballus 13Val Ala Ile Arg Asn Phe Asp Ile
Ser Lys Ile Ser Gly Glu Trp Tyr1 5 10 15Ser Ile Phe Leu Ala Ser Asp
Val Lys Glu Lys Ile Glu Glu Asn Gly 20 25 30Ser Met Arg Val Phe Val
Asp Val Ile Arg Ala Leu Asp Asn Ser Ser 35 40 45Leu Tyr Ala Glu Tyr
Gln Thr Lys Val Asn Gly Glu Cys Thr Glu Phe 50 55 60Pro Met Val Phe
Asp Lys Thr Glu Glu Asp Gly Val Tyr Ser Leu Asn65 70 75 80Tyr Asp
Gly Tyr Asn Val Phe Arg Ile Ser Glu Phe Glu Asn Asp Glu 85 90 95His
Ile Ile Leu Tyr Leu Val Asn Phe Asp Lys Asp Arg Pro Phe Gln 100 105
110Leu Phe Glu Phe Tyr Ala Arg Glu Pro Asp Val Ser Pro Glu Ile Lys
115 120 125Glu Glu Phe Val Lys Ile Val Gln Lys Arg Gly Ile Val Lys
Glu Asn 130 135 140Ile Ile Asp Leu Thr Lys Ile Asp Arg Cys Phe Gln
Leu Arg Gly145 150 15514269PRTArtificial SequenceSynthetic
construct 14Ala Gln Thr Ile Pro Trp Gly Ile Ser Arg Val Gln Ala Pro
Ala Ala1 5 10 15His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala
Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg
Gly Gly Ala Ser 35 40 45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly
Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn
Asn Ser Ile Gly Val Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu
Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Ser Gly Ser Gly Ser Val Ser
Ser Ile Ala Gln Gly Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met
His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala
Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val
Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150
155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp
Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly
Leu Asp Ile 180 185 190Met Ala Pro Gly Val Asn Ile Gln Ser Thr Tyr
Pro Gly Ser Thr Tyr 195 200 205Ala Ser Asp Asn Gly Thr Ser Met Ala
Thr Pro His Val Ala Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys
Asn Pro Ser Trp Ser Asn Val Gln Ile225 230 235 240Arg Asn His Leu
Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly
Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 26515129PRTGallus
gallus 15Lys Val Phe Gly Arg Cys Glu Leu Ala Ala Ala Met Lys Arg
His Gly1 5 10 15Leu Asp Asn Tyr Arg Gly Tyr Ser Leu Gly Asn Trp Val
Cys Ala Ala 20 25 30Lys Phe Glu Ser Asn Phe Asn Thr Gln Ala Thr Asn
Arg Asn Thr Asp 35 40 45Gly Ser Thr Asp Tyr Gly Ile Leu Gln Ile Asn
Ser Arg Trp Trp Cys 50 55 60Asn Asp Gly Arg Thr Pro Gly Ser Arg Asn
Leu Cys Asn Ile Pro Cys65 70 75 80Ser Ala Leu Leu Ser Ser Asp Ile
Thr Ala Ser Val Asn Cys Ala Lys 85 90 95Lys Ile Val Ser Asp Ala Asn
Gly Met Asn Ala Trp Val Ala Trp Arg 100 105 110Asn Arg Cys Lys Gly
Thr Asp Val Gln Ala Trp Ile Arg Gly Cys Arg 115 120 125Leu
16260PRTHavea brasiliensis 16Ser Trp Gln Thr Tyr Val Asp Asp His
Leu Met Cys Asp Ile Asp Gly1 5 10 15His Arg Leu Thr Ala Ala Ala Ile
Ile Gly His Asp Gly Ser Val Trp 20 25 30Ala Gln Ser Ser Ser Phe Pro
Gln Phe Lys Ser Asp Glu Val Ala Ala 35 40 45Val Met Lys Asp Phe Asp
Glu Pro Gly Ser Leu Ala Pro Thr Gly Leu 50 55 60His Leu Gly Gly Thr
Lys Tyr Met Val Ile Gln Gly Glu Pro Gly Ala65 70 75 80Val Ile Arg
Gly Lys Lys Gly Ser Gly Gly Ile Thr Val Lys Arg Thr 85 90 95Gly Gln
Ala Leu Ile Ile Gly Ile Tyr Asp Glu Pro Leu Thr Pro Gly 100 105
110Gln Cys Asn Met Ile Val Glu Arg Leu Gly Asp Tyr Leu Leu Asp Gln
115 120 125Gly Leu Ser Trp Gln Thr Tyr Val Asp Asp His Leu Met Cys
Asp Ile 130 135 140Asp Gly His Arg Leu Thr Ala Ala Ala Ile Ile Gly
His Asp Gly Ser145 150 155 160Val Trp Ala Gln Ser Ser Ser Phe Pro
Gln Phe Lys Ser Asp Glu Val 165 170 175Ala Ala Val Met Lys Asp Phe
Asp Glu Pro Gly Ser Leu Ala Pro Thr 180 185 190Gly Leu His Leu Gly
Gly Thr Lys Tyr Met Val Ile Gln Gly Glu Pro 195 200 205Gly Ala Val
Ile Arg Gly Lys Lys Gly Ser Gly Gly Ile Thr Val Lys 210 215 220Arg
Thr Gly Gln Ala Leu Ile Ile Gly Ile Tyr Asp Glu Pro Leu Thr225 230
235 240Pro Gly Gln Cys Asn Met Ile Val Glu Arg Leu Gly Asp Tyr Leu
Leu 245 250 255Asp Gln Gly Leu 26017125PRTAcanthamoeba castellanii
17Ser Trp Gln Thr Tyr Val Asp Thr Asn Leu Val Gly Thr Gly Ala Val1
5 10 15Thr Gln Ala Ala Ile Leu Gly Leu Asp Gly Asn Thr Trp Ala Thr
Ser 20 25 30Ala Gly Phe Ala Val Thr Pro Ala Gln Gly Gln Thr Leu Ala
Ser Ala 35 40 45Phe Asn Asn Ala Asp Pro Ile Arg Ala Ser Gly Phe Asp
Leu Ala Gly 50 55 60Val His Tyr Val Thr Leu Arg Ala Asp Asp Arg Ser
Ile Tyr Gly Lys65 70 75 80Lys Gly Ser Ala Gly Val Ile Thr Val Lys
Thr Ser Lys Ser Ile Leu 85 90 95Val Gly Val Tyr Asn Glu Lys Ile Gln
Pro Gly Thr Ala Ala Asn Val 100 105 110Val Glu Lys Leu Ala Asp Tyr
Leu Ile Gly Gln Gly Phe 115 120 12518130PRTArabidosis thaliana
18Ser Trp Gln Ser Tyr Val Asp Asp His Leu Met Cys Asp Val Glu Gly1
5 10 15Asn His Leu Thr Ala Ala Ala Ile Leu Gly Gln Asp Gly Ser Val
Trp 20 25 30Ala Gln Ser Ala Lys Phe Pro Gln Leu Lys Pro Gln Glu Ile
Asp Gly 35 40 45Ile Lys Lys Asp Phe Glu Glu Pro Gly Phe Leu Ala Pro
Thr Gly Leu 50 55 60Phe Leu Gly Gly Glu Lys Tyr Met Val Ile Gln Gly
Glu Gln Gly Ala65 70 75 80Val Ile Arg Gly Lys Lys Gly Pro Gly Gly
Val Thr Ile Lys Lys Thr 85 90 95Asn Gln Ala Leu Val Phe Gly Phe Tyr
Asp Glu Pro Met Thr Gly Gly 100 105 110Gln Cys Asn Leu Val Val Glu
Arg Leu Gly Asp Tyr Leu Ile Glu Ser 115 120 125Glu Leu
13019250PRTAcanthamoeba castellanii 19Ser Trp Gln Thr Tyr Val Asp
Thr Asn Leu Val Gly Thr Gly Ala Val1 5 10 15Thr Gln Ala Ala Ile Ile
Gly His Asp Gly Asn Thr Trp Ala Thr Ser 20 25 30Ala Gly Phe Ala Val
Ser Pro Ala Asn Gly Ala Ala Leu Ala Asn Ala 35 40 45Phe Lys Asp Ala
Thr Ala Ile Arg Ser Asn Gly Phe Glu Leu Ala Gly 50 55 60Thr Arg Tyr
Val Thr Ile Arg Ala Asp Asp Arg Ser Val Tyr Gly Lys65 70 75 80Lys
Gly Ser Ala Gly Val Ile Thr Val Lys Thr Ser Lys Ala Ile Leu 85 90
95Ile Gly Val Tyr Asn Glu Lys Ile Gln Pro Gly Thr Ala Ala Asn Val
100 105 110Val Glu Lys Leu Ala Asp Tyr Leu Ile Gly Gln Gly Phe Ser
Trp Gln 115 120 125Thr Tyr Val Asp Thr Asn Leu Val Gly Thr Gly Ala
Val Thr Gln Ala 130 135 140Ala Ile Ile Gly His Asp Gly Asn Thr Trp
Ala Thr Ser Ala Gly Phe145 150 155 160Ala Val Ser Pro Ala Asn Gly
Ala Ala Leu Ala Asn Ala Phe Lys Asp 165 170 175Ala Thr Ala Ile Arg
Ser Asn Gly Phe Glu Leu Ala Gly Thr Arg Tyr 180 185 190Val Thr Ile
Arg Ala Asp Asp Arg Ser Val Tyr Gly Lys Lys Gly Ser 195 200 205Ala
Gly Val Ile Thr Val Lys Thr Ser Lys Ala Ile Leu Ile Gly Val 210 215
220Tyr Asn Glu Lys Ile Gln Pro Gly Thr Ala Ala Asn Val Val Glu
Lys225 230 235 240Leu Ala Asp Tyr Leu Ile Gly Gln Gly Phe 245
25020123PRTBetula pendula 20Ser Trp Gln Thr Tyr Val Asp Glu His Leu
Met Leu Ala Ala Ser Ala1 5 10 15Ile Val Gly His Asp Gly Ser Val Trp
Ala Gln Ser Ser Ser Phe Pro 20 25 30Gln Phe Lys Pro Gln Glu Ile Thr
Gly Ile Met Lys Asp Phe Glu Glu 35 40 45Pro Gly His Leu Ala Pro Thr
Gly Leu His Leu Gly Gly Ile Lys Tyr 50 55 60Met Val Ile Gln Gly Glu
Ala Gly Ala Val Ile Arg Gly Lys Lys Gly65 70 75 80Ser Gly Gly Ile
Thr Ile Lys Lys Thr Gly Gln Ala Leu Val Phe Gly 85 90 95Ile Tyr Glu
Glu Pro Val Thr Pro Gly Gln Cys Asn Met Val Val Glu 100 105 110Arg
Leu Gly Asp Tyr Leu Ile Asp Gln Gly Leu 115 1202140PRTAmbrosia
trifida 21Asp Asp Gly Leu Cys Tyr Glu Gly Thr Asn Cys Gly Lys Val
Gly Lys1 5 10 15Tyr Cys Cys Ser Pro Ile Gly Lys Tyr Cys Val Cys Tyr
Asp Ser Lys 20 25 30Ala Ile Cys Asn Lys Asn Cys Thr 35
4022209PRTVespula vulgaris 22Ala Glu Ala Glu Phe Asn Asn Tyr Cys
Lys Ile Lys Cys Leu Lys Gly1 5 10 15Gly Val His Thr Ala Cys Lys Tyr
Gly Ser Leu Lys Pro Asn Cys Gly 20 25 30Asn Lys Val Val Val Ser Tyr
Gly Leu Thr Lys Gln Glu Lys Gln Asp 35 40 45Ile Leu Lys Glu His Asn
Asp Phe Arg Gln Lys Ile Ala Arg Gly Leu 50 55 60Glu Thr Arg Gly Asn
Pro Gly Pro Gln Pro Pro Ala Lys Asn Met Lys65 70 75 80Asn Leu Val
Trp Asn Asp Glu Leu Ala Tyr Val Ala Gln Val Trp Ala 85 90 95Asn Gln
Cys Gln Tyr Gly His Asp Thr Cys Arg Asp Val Ala Lys Tyr 100 105
110Gln Val Gly Gln Asn Val Ala Leu Thr Gly Ser Thr Ala Ala Lys Tyr
115 120 125Asp Asp Pro Val Lys Leu Val Lys Met Trp Glu Asp Glu Val
Lys Asp 130 135 140Tyr Asn Pro Lys Lys Lys Phe Ser Gly Asn Asp Phe
Leu Lys Thr Gly145 150 155 160His Tyr Thr Gln Met Val Trp Ala Asn
Thr Lys Glu Val Gly Cys Gly 165 170 175Ser Ile Lys Tyr Ile Gln Glu
Lys Trp His Lys His Tyr Leu Val Cys 180 185 190Asn Tyr Gly Pro Ser
Gly Asn Phe Lys Asn Glu Glu Leu Tyr Gln Thr 195 200 205Lys
23269PRTArtificial SequenceSynthetic construct 23Ala Gln Thr Ile
Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg
Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly
Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe
Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55
60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65
70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly
Ala 85 90 95Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu
Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu
Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn
Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly
Asn Ser Gly Ala Gly Ser Ile Ser145 150
155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp
Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly
Leu Asp Ile 180 185 190Met Ala Pro Gly Val Asn Ile Gln Ser Thr Tyr
Pro Gly Ser Thr Tyr 195 200 205Ala Ser Asp Asn Gly Thr Ser Met Ala
Thr Pro His Val Ala Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys
Asn Pro Ser Trp Ser Asn Val Gln Ile225 230 235 240Arg Asn His Leu
Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly
Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 26524269PRTBacillus
lentus 24Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro
Ala Ala1 5 10 15His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala
Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg
Gly Gly Ala Ser 35 40 45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly
Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn
Asn Ser Ile Gly Val Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu
Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Ser Gly Ser Gly Ser Val Ser
Ser Ile Ala Gln Gly Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met
His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala
Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val
Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150
155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp
Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly
Leu Asp Ile 180 185 190Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr
Pro Gly Ser Thr Tyr 195 200 205Ala Ser Leu Asn Gly Thr Ser Met Ala
Thr Pro His Val Ala Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys
Asn Pro Ser Trp Ser Asn Val Gln Ile225 230 235 240Arg Asn His Leu
Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly
Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 26525274PRTBacillus
licheniformis 25Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala
Asp Lys Val1 5 10 15Gln Ala Gln Gly Phe Lys Gly Ala Asn Val Lys Val
Ala Val Leu Asp 20 25 30Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn
Val Val Gly Gly Ala 35 40 45Ser Phe Val Ala Gly Glu Ala Tyr Asn Thr
Asp Gly Asn Gly His Gly 50 55 60Thr His Val Ala Gly Thr Val Ala Ala
Leu Asp Asn Thr Thr Gly Val65 70 75 80Leu Gly Val Ala Pro Ser Val
Ser Leu Tyr Ala Val Lys Val Leu Asn 85 90 95Ser Ser Gly Ser Gly Ser
Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 100 105 110Ala Thr Thr Asn
Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Ala 115 120 125Ser Gly
Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 130 135
140Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly
Asn145 150 155 160Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser
Val Ile Ala Val 165 170 175Gly Ala Val Asp Ser Asn Ser Asn Arg Ala
Ser Phe Ser Ser Val Gly 180 185 190Ala Glu Leu Glu Val Met Ala Pro
Gly Ala Gly Val Tyr Ser Thr Tyr 195 200 205Pro Thr Asn Thr Tyr Ala
Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 210 215 220His Val Ala Gly
Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu225 230 235 240Ser
Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 245 250
255Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala
260 265 270Ala Gln26269PRTArtificial SequenceSynthetic construct
26Ala Gln Thr Ile Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1
5 10 15His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu
Asp 20 25 30Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly
Ala Ser 35 40 45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly
His Gly Thr 50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser
Ile Gly Val Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala
Val Lys Val Leu Gly Ala 85 90 95Ser Gly Ser Gly Ser Val Ser Ser Ile
Ala Gln Gly Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met His Val
Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu
Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val
Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150 155
160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln
165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu
Asp Ile 180 185 190Met Ala Pro Gly Val Asn Ile Gln Ser Thr Tyr Pro
Gly Ser Thr Tyr 195 200 205Ala Ser Asp Asn Gly Thr Ser Met Ala Thr
Pro His Val Ala Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys Asn
Pro Ser Trp Ser Asn Val Gln Ile225 230 235 240Arg Asn His Leu Lys
Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser
Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 26527269PRTArtificial
SequenceSynthetic construct 27Ala Gln Ser Val Pro Trp Gly Ile Ser
Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu Thr Gly Ser
Gly Val Arg Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro
Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe Val Pro Gly Glu Pro
Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr
Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65 70 75 80Gly Val Ala
Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Ser Gly
Ser Gly Ser Tyr Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 100 105
110Gly Asn Asn Gly Met His Val Ala Ser Leu Ser Leu Gly Ser Pro Ser
115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser
Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala
Gly Ser Ile Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met
Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe
Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185 190Val Ala Pro Gly Val
Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205Ala Ser Leu
Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215 220Ala
Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile225 230
235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn
Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Ala Arg
260 26528269PRTArtificial SequenceSynthetic construct 28Ala Gln Ser
Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn
Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr
Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40
45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr
50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Ser Ile Gly Val
Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val
Leu Gly Ala 85 90 95Ser Gly Ser Gly Ala Ile Ser Ser Ile Ala Gln Gly
Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu
Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala
Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala
Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala
Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn
Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185
190Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr
195 200 205Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala
Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser
Asn Val Gln Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr
Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn
Ala Glu Ala Ala Thr Arg 260 26529269PRTArtificial SequenceSynthetic
construct 29Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro
Ala Ala1 5 10 15His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala
Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg
Gly Gly Ala Ser 35 40 45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly
Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn
Asn Ser Ile Gly Val Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu
Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Ser Gly Gly Gly Ala Ile Ser
Ser Ile Ala Gln Gly Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met
His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala
Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val
Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Asp Ser Ile Ser145 150
155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp
Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly
Leu Asp Ile 180 185 190Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr
Pro Gly Ser Thr Tyr 195 200 205Ala Ser Leu Asn Gly Thr Ser Met Ala
Thr Pro His Val Ala Gly Ala 210 215 220Ala Val Leu Val Lys His Lys
Asn Pro Ser Trp Ser Asn Val Arg Ile225 230 235 240Arg Asp His Leu
Lys Lys Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly
Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260
26530269PRTArtificial SequenceSynthetic construct 30Ala Gln Ser Val
Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg
Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly
Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe
Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55
60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65
70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly
Ala 85 90 95Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu
Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu
Gly Ser Pro Ser 115 120 125Ala Gly Gly Thr Leu Glu Gln Ala Val Asn
Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly
Asn Ser Gly Ala Gly Ser Ile Ser145 150 155 160Ala Pro Ala Ser Tyr
Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn
Arg Ala Ser Phe Ser Gln Tyr Gly Pro Gly Leu Asp Ile 180 185 190Val
Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200
205Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala
210 215 220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val
Gln Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu
Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu
Ala Ala Thr Arg 260 26531269PRTArtificial SequenceSynthetic
construct 31Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro
Ala Ala1 5 10 15His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala
Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg
Gly Gly Ala Ser 35 40 45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly
Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn
Asn Ser Ile Gly Val Leu65 70 75 80Gly Val Ala Pro Asn Ala Glu Leu
Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Ser Gly Gly Gly Ser Asn Ser
Ser Ile Ala Gln Gly Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met
His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala
Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val
Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150
155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp
Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly
Leu Asp Ile 180 185 190Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr
Pro Gly Ser Thr Tyr 195 200 205Ala Ser Leu Asn Gly Thr Ser Met Ala
Thr Pro His Val Ala Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys
Asn Pro Ser Trp Ser Asn Val Gln Ile225 230 235 240Arg Asn His Leu
Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly
Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260
26532270PRTArtificial SequenceSynthetic construct 32Ala Gln Ser Val
Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg
Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly
Ile Asp Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala 35 40 45Ser
Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly 50 55
60Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Ser Ile Gly Val65
70 75 80Leu Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu
Gly 85 90 95Ala Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu
Glu Trp 100 105 110Ala Gly Asn Asn Gly Met Asp Val Ala Asn Leu Ser
Leu Gly Ser Pro 115 120 125Ser Pro Ser Ala Thr Leu Glu Gln Ala Val
Asn Ser Ala Thr Ser Arg 130 135 140Gly Val Leu Val Val Ala Ala Ser
Gly Asn Ser Gly Ala Gly Ser
Ile145 150 155 160Ser Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val
Gly Ala Thr Asp 165 170 175Gln Asn Asn Asn Arg Ala Ser Phe Ser Gln
Tyr Gly Ala Glu Leu Asp 180 185 190Ile Val Ala Pro Gly Val Asn Val
Gln Ser Thr Tyr Pro Gly Ser Thr 195 200 205Tyr Ala Ser Leu Asn Gly
Thr Ser Met Ala Thr Pro His Val Ala Gly 210 215 220Ala Ala Ala Leu
Val Leu Gln Lys Asn Pro Ser Trp Ser Asn Val Gln225 230 235 240Ile
Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn 245 250
255Leu Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 265
27033280PRTBacillus species. 33Trp Ser Pro Asn Asp Pro Tyr Tyr Ser
Ala Tyr Gln Tyr Gly Pro Gln1 5 10 15Asn Thr Ser Thr Pro Ala Ala Trp
Asp Val Thr Arg Gly Ser Ser Thr 20 25 30Gln Thr Val Ala Val Leu Asp
Ser Gly Val Asp Tyr Asn His Pro Asp 35 40 45Leu Ala Arg Lys Val Ile
Lys Gly Tyr Asp Phe Ile Asp Arg Asp Asn 50 55 60Asn Pro Met Asp Leu
Asn Gly His Gly Thr His Val Ala Gly Thr Val65 70 75 80Ala Ala Asp
Thr Asn Asn Gly Ile Gly Val Ala Gly Met Ala Pro Asp 85 90 95Thr Lys
Ile Leu Ala Val Arg Val Leu Asp Ala Asn Gly Ser Gly Ser 100 105
110Leu Asp Ser Ile Ala Ser Gly Ile Arg Tyr Ala Ala Asp Gln Gly Ala
115 120 125Lys Val Leu Asn Leu Ser Leu Gly Cys Glu Cys Asn Ser Thr
Thr Leu 130 135 140Lys Ser Ala Val Asp Tyr Ala Trp Asn Lys Gly Ala
Val Val Val Ala145 150 155 160Ala Ala Gly Asn Asp Asn Val Ser Arg
Thr Phe Gln Pro Ala Ser Tyr 165 170 175Pro Asn Ala Ile Ala Val Gly
Ala Ile Asp Ser Asn Asp Arg Lys Ala 180 185 190Ser Phe Ser Asn Tyr
Gly Thr Trp Val Asp Val Thr Ala Pro Gly Val 195 200 205Asn Ile Ala
Ser Thr Val Pro Asn Asn Gly Tyr Ser Tyr Met Ser Gly 210 215 220Thr
Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala Leu Leu Ala225 230
235 240Ser Gln Gly Lys Asn Asn Val Gln Ile Arg Gln Ala Ile Glu Gln
Thr 245 250 255Ala Asp Lys Ile Ser Gly Thr Gly Thr Asn Phe Lys Tyr
Gly Lys Ile 260 265 270Asn Ser Asn Lys Ala Val Arg Tyr 275
28034269PRTBacillus species. 34Asn Gln Val Thr Pro Trp Gly Ile Thr
Arg Val Gln Ala Pro Thr Ala1 5 10 15Trp Thr Arg Gly Tyr Thr Gly Thr
Gly Val Arg Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr His Pro
Asp Leu Asn Ile Arg Gly Gly Val Ser 35 40 45Phe Val Pro Gly Glu Pro
Ser Tyr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala Gly Thr
Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Val65 70 75 80Gly Val Ala
Pro Asn Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90 95Asn Gly
Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Gln Trp Thr 100 105
110Ala Gln Asn Asn Ile His Val Ala Asn Leu Ser Leu Gly Ser Pro Val
115 120 125Gly Ser Gln Thr Leu Glu Leu Ala Val Asn Gln Ala Thr Asn
Ala Gly 130 135 140Val Leu Val Val Ala Ala Thr Gly Asn Asn Gly Ser
Gly Thr Val Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn Ala Leu
Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala Ser Phe
Ser Gln Tyr Gly Thr Gly Leu Asn Ile 180 185 190Val Ala Pro Gly Val
Gly Ile Gln Ser Thr Tyr Pro Gly Asn Arg Tyr 195 200 205Ala Ser Leu
Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 210 215 220Ala
Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Thr Gln Ile225 230
235 240Arg Gln His Leu Thr Ser Thr Ala Thr Ser Leu Gly Asn Ser Asn
Gln 245 250 255Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg
260 26535268PRTBacillus species. 35Gln Thr Val Pro Trp Gly Ile Asn
Arg Val Gln Ala Pro Ile Ala Gln1 5 10 15Ser Arg Gly Phe Thr Gly Thr
Gly Val Arg Val Ala Val Leu Asp Thr 20 25 30Gly Ile Ser Asn His Ala
Asp Leu Arg Ile Arg Gly Gly Ala Ser Phe 35 40 45Val Pro Gly Glu Pro
Asn Ile Ser Asp Gly Asn Gly His Gly Thr Gln 50 55 60Val Ala Gly Thr
Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly65 70 75 80Val Ala
Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala Ser 85 90 95Gly
Ser Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala Ala 100 105
110Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala Gly
115 120 125Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser
Gly Val 130 135 140Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly
Asn Val Gly Phe145 150 155 160Pro Ala Arg Tyr Ala Asn Ala Met Ala
Val Gly Ala Thr Asp Gln Asn 165 170 175Asn Asn Arg Ala Thr Phe Ser
Gln Tyr Gly Ala Gly Leu Asp Ile Val 180 185 190Ala Pro Gly Val Gly
Val Gln Ser Thr Val Pro Gly Asn Gly Tyr Ala 195 200 205Ser Phe Asn
Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val Ala 210 215 220Ala
Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile Arg225 230
235 240Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln
Phe 245 250 255Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260
26536471PRTAsergillus niger 36Ala Thr Leu Asp Ser Trp Leu Ser Asn
Glu Ala Thr Val Ala Arg Thr1 5 10 15Ala Ile Leu Asn Asn Ile Gly Ala
Asp Gly Ala Trp Val Ser Gly Ala 20 25 30Asp Ser Gly Ile Val Val Ala
Ser Pro Ser Thr Asp Asn Pro Asp Tyr 35 40 45Phe Tyr Thr Trp Thr Arg
Asp Ser Gly Leu Val Leu Lys Thr Leu Val 50 55 60Asp Leu Phe Arg Asn
Gly Asp Thr Ser Leu Leu Ser Thr Ile Glu Asn65 70 75 80Tyr Ile Ser
Ala Gln Ala Ile Val Gln Gly Ile Ser Asn Pro Ser Gly 85 90 95Asp Leu
Ser Ser Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp 100 105
110Glu Thr Ala Tyr Thr Gly Ser Trp Gly Arg Pro Gln Arg Asp Gly Pro
115 120 125Ala Leu Arg Ala Thr Ala Met Ile Gly Phe Gly Gln Trp Leu
Leu Asp 130 135 140Asn Gly Tyr Thr Ser Thr Ala Thr Asp Ile Val Trp
Pro Leu Val Arg145 150 155 160Asn Asp Leu Ser Tyr Val Ala Gln Tyr
Trp Asn Gln Thr Gly Tyr Asp 165 170 175Leu Trp Glu Glu Val Asn Gly
Ser Ser Phe Phe Thr Ile Ala Val Gln 180 185 190His Arg Ala Leu Val
Glu Gly Ser Ala Phe Ala Thr Ala Val Gly Ser 195 200 205Ser Cys Ser
Trp Cys Asp Ser Gln Ala Pro Glu Ile Leu Cys Tyr Leu 210 215 220Gln
Ser Phe Trp Thr Gly Ser Phe Ile Leu Ala Asn Phe Asp Ser Ser225 230
235 240Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu Gly Ser Ile His Thr
Phe 245 250 255Asp Pro Glu Ala Ala Cys Asp Asp Ser Thr Phe Gln Pro
Cys Ser Pro 260 265 270Arg Ala Leu Ala Asn His Lys Glu Val Val Asp
Ser Phe Arg Ser Ile 275 280 285Tyr Thr Leu Asn Asp Gly Leu Ser Asp
Ser Glu Ala Val Ala Val Gly 290 295 300Arg Tyr Pro Glu Asp Thr Tyr
Tyr Asn Gly Asn Pro Trp Phe Leu Cys305 310 315 320Thr Leu Ala Ala
Ala Glu Gln Leu Tyr Asp Ala Leu Tyr Gln Trp Asp 325 330 335Lys Gln
Gly Ser Leu Glu Val Thr Asp Val Ser Leu Asp Phe Phe Lys 340 345
350Ala Leu Tyr Ser Asp Ala Ala Thr Gly Thr Tyr Ser Ser Ser Ser Ser
355 360 365Thr Tyr Ser Ser Ile Val Asp Ala Val Lys Thr Phe Ala Asp
Gly Phe 370 375 380Val Ser Ile Val Glu Thr His Ala Ala Ser Asn Gly
Ser Met Ser Glu385 390 395 400Gln Tyr Asp Lys Ser Asp Gly Glu Gln
Leu Ser Ala Arg Asp Leu Thr 405 410 415Trp Ser Tyr Ala Ala Leu Leu
Thr Ala Asn Asn Arg Arg Asn Ser Val 420 425 430Val Pro Ala Ser Trp
Gly Glu Thr Ser Ala Ser Ser Val Pro Gly Thr 435 440 445Cys Ala Ala
Thr Ser Ala Ile Gly Thr Tyr Ser Ser Val Thr Val Thr 450 455 460Ser
Trp Pro Ser Ile Val Ala465 47037480PRTBacillus species. 37Thr Asn
Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu Pro Asn Asp1 5 10 15Gly
Asn His Trp Asn Arg Leu Arg Ser Asp Ala Ser Asn Leu Lys Asp 20 25
30Lys Gly Ile Ser Ala Val Trp Ile Pro Pro Ala Trp Lys Gly Ala Ser
35 40 45Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu Gly
Glu 50 55 60Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly Thr Arg
Asn Gln65 70 75 80Leu Gln Ala Ala Val Asn Ala Leu Lys Ser Asn Gly
Ile Gln Val Tyr 85 90 95Gly Asp Val Val Met Asn His Lys Gly Gly Ala
Asp Ala Thr Glu Met 100 105 110Val Arg Ala Val Glu Val Asn Pro Asn
Asn Arg Asn Gln Glu Val Ser 115 120 125Gly Glu Tyr Thr Ile Glu Ala
Trp Thr Lys Phe Asp Phe Pro Gly Arg 130 135 140Gly Asn Thr His Ser
Asn Phe Lys Trp Arg Trp Tyr His Phe Asp Gly145 150 155 160Val Asp
Trp Asp Gln Ser Arg Lys Leu Asn Asn Arg Ile Tyr Lys Phe 165 170
175Arg Gly Asp Gly Lys Gly Trp Asp Trp Glu Val Asp Thr Glu Asn Gly
180 185 190Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met Asp His
Pro Glu 195 200 205Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr
Thr Asn Thr Leu 210 215 220Gly Leu Asp Gly Phe Arg Ile Asp Ala Val
Lys His Ile Lys Tyr Ser225 230 235 240Phe Thr Arg Asp Trp Ile Asn
His Val Arg Ser Ala Thr Gly Lys Asn 245 250 255Met Phe Ala Val Ala
Glu Phe Trp Lys Asn Asp Leu Gly Ala Ile Glu 260 265 270Asn Tyr Leu
Asn Lys Thr Asn Trp Asn His Ser Val Phe Asp Val Pro 275 280 285Leu
His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly Gly Asn Tyr Asp 290 295
300Met Arg Gln Ile Phe Asn Gly Thr Val Val Gln Arg His Pro Met
His305 310 315 320Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro
Glu Glu Ala Leu 325 330 335Glu Ser Phe Val Glu Glu Trp Phe Lys Pro
Leu Ala Tyr Ala Leu Thr 340 345 350Leu Thr Arg Glu Gln Gly Tyr Pro
Ser Val Phe Tyr Gly Asp Tyr Tyr 355 360 365Gly Ile Pro Thr His Gly
Val Pro Ala Met Lys Ser Lys Ile Asp Pro 370 375 380Ile Leu Glu Ala
Arg Gln Lys Tyr Ala Tyr Gly Arg Gln Asn Asp Tyr385 390 395 400Leu
Asp His His Asn Ile Ile Gly Trp Thr Arg Glu Gly Asn Thr Ala 405 410
415His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly Ala Gly Gly
420 425 430Asn Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly Gln Val
Trp Thr 435 440 445Asp Ile Thr Gly Asn Arg Ala Gly Thr Val Thr Ile
Asn Ala Asp Gly 450 455 460Trp Gly Asn Phe Ser Val Asn Gly Gly Ser
Val Ser Ile Trp Val Asn465 470 475 480384PRTArtificial
SequenceSynthetic construct 38Arg Arg Xaa Ser1395PRTArtificial
SequenceSynthetic construct 39Arg Arg Xaa Xaa Ser1
54011PRTArtificial SequenceSynthetic construct 40Lys Arg Xaa Xaa
Xaa Asp Glu Xaa Xaa Xaa Tyr1 5 10415PRTArtificial SequenceSynthetic
construct 41Lys Ala Ala Lys Asp1 5425PRTArtificial
SequenceSynthetic construct 42Lys Leu Ala Ser Asp1
5435PRTArtificial SequenceSynthetic construct 43Lys Leu Tyr Ser
Asp1 5444PRTArtificial SequenceSynthetic construct 44Lys Leu Tyr
Asp145274PRTBacillus 45Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile
Lys Ala Asp Lys Val1 5 10 15Gln Ala Gln Gly Phe Lys Gly Ala Asn Val
Lys Val Ala Val Leu Asp 20 25 30Thr Gly Ile Gln Ala Ser His Pro Asp
Leu Asn Val Val Gly Gly Ala 35 40 45Ser Phe Val Ala Gly Glu Ala Tyr
Asn Thr Asp Gly Asn Gly His Gly 50 55 60Thr His Val Ala Gly Thr Val
Ala Ala Leu Asp Asn Thr Thr Gly Val65 70 75 80Leu Gly Val Ala Pro
Ser Val Ser Leu Tyr Ala Val Lys Val Leu Asn 85 90 95Ser Ser Gly Ser
Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 100 105 110Ala Thr
Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Ala 115 120
125Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg
130 135 140Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser
Gly Asn145 150 155 160Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp
Ser Val Ile Ala Val 165 170 175Gly Ala Val Asp Ser Asn Ser Asn Arg
Ala Ser Phe Ser Ser Val Gly 180 185 190Ala Glu Leu Glu Val Met Ala
Pro Gly Ala Gly Val Tyr Ser Thr Tyr 195 200 205Pro Thr Asn Thr Tyr
Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 210 215 220His Val Ala
Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu225 230 235
240Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu
245 250 255Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu
Ala Ala 260 265 270Ala Gln46275PRTBacillus 46Ala Gln Ser Val Pro
Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu1 5 10 15His Ser Gln Gly
Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 20 25 30Ser Gly Ile
Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 35 40 45Ser Met
Val Pro Ser Glu Thr Pro Asn Phe Gln Asp Asp Asn Ser His 50 55 60Gly
Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly65 70 75
80Val Leu Gly Val Ala Pro Ser Ser Ala Leu Tyr Ala Val Lys Val Leu
85 90 95Gly Asp Ala Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile
Glu 100 105 110Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Met Ser
Leu Gly Gly 115 120 125Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val
Asp Lys Ala Val Ala 130 135 140Ser Gly Val Val Val Val Ala Ala Ala
Gly Asn Glu Gly Ser Thr Gly145 150 155 160Ser Ser Ser Thr Val Gly
Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 165 170 175Val Gly Ala Val
Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val 180 185 190Gly Pro
Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 195 200
205Leu Pro Gly Asn
Lys Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser 210 215 220Pro His
Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn225 230 235
240Trp Thr Asn Thr Gln Val Arg Ser Ser Leu Gln Asn Thr Thr Thr Lys
245 250 255Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val
Gln Ala 260 265 270Ala Ala Gln 27547269PRTBacillus 47Ala Gln Thr
Ile Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn
Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr
Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40
45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr
50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val
Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val
Leu Gly Ala 85 90 95Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly
Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu
Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala
Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala
Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala
Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn
Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185
190Met Ala Pro Gly Val Asn Ile Gln Ser Thr Tyr Pro Gly Ser Thr Tyr
195 200 205Ala Ser Asp Asn Gly Thr Ser Met Ala Thr Pro His Val Ala
Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser
Asn Val Gln Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr
Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn
Ala Glu Ala Ala Thr Arg 260 26548268PRTBacillus 48Gln Thr Val Pro
Trp Gly Ile Ser Phe Ile Asn Thr Gln Gln Ala His1 5 10 15Asn Arg Gly
Ile Phe Gly Asn Gly Ala Arg Val Ala Val Leu Asp Thr 20 25 30Gly Ile
Ala Ser His Pro Asp Leu Arg Ile Ala Gly Gly Ala Ser Phe 35 40 45Ile
Ser Ser Glu Pro Ser Tyr His Asp Asn Asn Gly His Gly Thr His 50 55
60Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly65
70 75 80Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Asp Arg
Asn 85 90 95Gly Ser Gly Ser Leu Ala Ser Val Ala Gln Gly Ile Glu Trp
Ala Ile 100 105 110Asn Asn Asn Met His Ile Ile Asn Met Ser Leu Gly
Ser Thr Ser Gly 115 120 125Ser Ser Thr Leu Glu Leu Ala Val Asn Arg
Ala Asn Asn Ala Gly Ile 130 135 140Leu Leu Val Gly Ala Ala Gly Asn
Thr Gly Arg Gln Gly Val Asn Tyr145 150 155 160Pro Ala Arg Tyr Ser
Gly Val Met Ala Val Ala Ala Val Asp Gln Asn 165 170 175Gly Gln Arg
Ala Ser Phe Ser Thr Tyr Gly Pro Glu Ile Glu Ile Ser 180 185 190Ala
Pro Gly Val Asn Val Asn Ser Thr Tyr Thr Gly Asn Arg Tyr Val 195 200
205Ser Leu Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val Ala
210 215 220Ala Leu Val Lys Ser Arg Tyr Pro Ser Tyr Thr Asn Asn Gln
Ile Arg225 230 235 240Gln Arg Ile Asn Gln Thr Ala Thr Tyr Leu Gly
Ser Pro Ser Leu Tyr 245 250 255Gly Asn Gly Leu Val His Ala Gly Arg
Ala Thr Gln 260 26549269PRTBacillus 49Ala Gln Ser Val Pro Trp Gly
Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu Thr
Gly Ser Gly Val Arg Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr
His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe Val Pro Gly
Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala
Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65 70 75 80Gly
Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90
95Ser Gly Ser Gly Ser Tyr Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala
100 105 110Gly Asn Asn Gly Met His Val Ala Ser Leu Ser Leu Gly Ser
Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala
Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly Asn Ser
Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn
Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala
Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185 190Val Ala Pro
Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205Ala
Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215
220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln
Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly
Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Ala Arg 260 26550269PRTBacillus 50Ala Gln Ser Val Pro Trp Gly
Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu Thr
Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr
His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe Val Pro Gly
Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala
Gly Thr Ile Ala Ala Leu Asp Asn Ser Ile Gly Val Leu65 70 75 80Gly
Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90
95Ser Gly Ser Gly Ala Ile Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala
100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser
Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala
Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly Asn Ser
Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn
Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala
Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185 190Val Ala Pro
Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205Ala
Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215
220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln
Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly
Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Thr Arg 260 26551269PRTBacillus 51Ala Gln Ser Val Pro Trp Gly
Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu Thr
Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr
His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe Val Pro Gly
Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala
Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65 70 75 80Gly
Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90
95Ser Gly Gly Gly Ala Ile Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala
100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser
Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala
Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly Asn Ser
Gly Ala Asp Ser Ile Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn
Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala
Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185 190Val Ala Pro
Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205Ala
Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215
220Ala Val Leu Val Lys His Lys Asn Pro Ser Trp Ser Asn Val Arg
Ile225 230 235 240Arg Asp His Leu Lys Lys Thr Ala Thr Ser Leu Gly
Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Thr Arg 260 26552269PRTBacillus 52Ala Gln Ser Val Pro Trp Gly
Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu Thr
Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr
His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe Val Pro Gly
Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala
Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65 70 75 80Gly
Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90
95Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala
100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser
Pro Ser 115 120 125Ala Gly Gly Thr Leu Glu Gln Ala Val Asn Ser Ala
Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly Asn Ser
Gly Ala Gly Ser Ile Ser145 150 155 160Ala Pro Ala Ser Tyr Ala Asn
Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala
Ser Phe Ser Gln Tyr Gly Pro Gly Leu Asp Ile 180 185 190Val Ala Pro
Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205Ala
Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215
220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln
Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly
Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Thr Arg 260 26553271PRTBacillus 53Trp Ser Pro Asn Asp Pro Tyr
Tyr Ser Ala Tyr Gln Tyr Gly Pro Gln1 5 10 15Asn Thr Ser Thr Pro Ala
Ala Trp Asp Val Thr Arg Gly Ser Ser Thr 20 25 30Gln Thr Val Ala Val
Leu Asp Ser Gly Val Asp Tyr Asn His Pro Asp 35 40 45Leu Ala Arg Lys
Val Ile Lys Gly Tyr Asp Phe Ile Asp Arg Asp Asn 50 55 60Asn Pro Met
Asp Leu Asn Gly His Gly Thr His Val Ala Gly Thr Val65 70 75 80Ala
Ala Asp Thr Asn Asn Gly Ile Gly Val Ala Gly Met Ala Pro Asp 85 90
95Thr Lys Ile Leu Ala Val Arg Val Leu Asp Ala Asn Gly Ser Gly Ser
100 105 110Leu Asp Ser Ile Ala Ser Gly Ile Arg Tyr Ala Ala Asp Gln
Gly Ala 115 120 125Lys Val Leu Asn Leu Ser Leu Gly Cys Glu Cys Asn
Ser Thr Thr Leu 130 135 140Lys Ser Ala Val Asp Tyr Ala Trp Asn Lys
Gly Ala Val Val Val Ala145 150 155 160Ala Ala Gly Asn Asp Asn Val
Ser Arg Thr Phe Gln Pro Ala Ser Tyr 165 170 175Pro Asn Ala Ile Ala
Val Gly Ala Ile Asp Ser Asn Asp Arg Lys Ala 180 185 190Ser Phe Ser
Asn Tyr Gly Thr Trp Val Asp Val Thr Ala Pro Gly Val 195 200 205Asn
Ile Ala Ser Thr Val Pro Asn Asn Gly Tyr Ser Tyr Met Ser Gly 210 215
220Thr Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala Leu Leu
Ala225 230 235 240Ser Gln Gly Lys Asn Asn Val Gln Ile Arg Gln Ala
Ile Glu Gln Thr 245 250 255Ala Asp Lys Ile Ser Gly Thr Gly Thr Asn
Phe Lys Tyr Gly Lys 260 265 27054269PRTBacillus 54Ala Gln Ser Val
Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg
Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly
Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45Phe
Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 50 55
60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu65
70 75 80Gly Val Ala Pro Asn Ala Glu Leu Tyr Ala Val Lys Val Leu Gly
Ala 85 90 95Ser Gly Gly Gly Ser Asn Ser Ser Ile Ala Gln Gly Leu Glu
Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu
Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala Val Asn
Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala Ser Gly
Asn Ser Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala Arg Tyr
Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn
Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185 190Val
Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200
205Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala
210 215 220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val
Gln Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu
Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn Ala Glu
Ala Ala Thr Arg 260 26555270PRTBacillus 55Ala Gln Ser Val Pro Trp
Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn Arg Gly Leu
Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr Gly Ile Asp
Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala 35 40 45Ser Phe Val
Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly 50 55 60Thr His
Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Ser Ile Gly Val65 70 75
80Leu Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly
85 90 95Ala Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu
Trp 100 105 110Ala Gly Asn Asn Gly Met Asp Val Ala Asn Leu Ser Leu
Gly Ser Pro 115 120 125Ser Pro Ser Ala Thr Leu Glu Gln Ala Val Asn
Ser Ala Thr Ser Arg 130 135 140Gly Val Leu Val Val Ala Ala Ser Gly
Asn Ser Gly Ala Gly Ser Ile145 150 155 160Ser Tyr Pro Ala Arg Tyr
Ala Asn Ala Met Ala Val Gly Ala Thr Asp 165 170 175Gln Asn Asn Asn
Arg Ala Ser Phe Ser Gln Tyr Gly Ala Glu Leu Asp 180 185 190Ile Val
Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr 195 200
205Tyr Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly
210 215 220Ala Ala Ala Leu Val Leu Gln Lys Asn Pro Ser Trp Ser Asn
Val Gln225 230 235 240Ile Arg Asn His Leu Lys Asn Thr Ala Thr
Ser Leu Gly Ser Thr Asn 245 250 255Leu Tyr Gly Ser Gly Leu Val Asn
Ala Glu Ala Ala Thr Arg 260 265 27056269PRTBacillus 56Ala Gln Ser
Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala1 5 10 15His Asn
Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30Thr
Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40
45Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr
50 55 60His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val
Leu65 70 75 80Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val
Leu Gly Ala 85 90 95Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly
Leu Glu Trp Ala 100 105 110Gly Asn Asn Gly Met His Val Ala Asn Leu
Ser Leu Gly Ser Pro Ser 115 120 125Pro Ser Ala Thr Leu Glu Gln Ala
Val Asn Ser Ala Thr Ser Arg Gly 130 135 140Val Leu Val Val Ala Ala
Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser145 150 155 160Tyr Pro Ala
Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 165 170 175Asn
Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 180 185
190Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr
195 200 205Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala
Gly Ala 210 215 220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser
Asn Val Gln Ile225 230 235 240Arg Asn His Leu Lys Asn Thr Ala Thr
Ser Leu Gly Ser Thr Asn Leu 245 250 255Tyr Gly Ser Gly Leu Val Asn
Ala Glu Ala Ala Thr Arg 260 26557268PRTBacillus 57Gln Thr Val Pro
Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala Gln1 5 10 15Ser Arg Gly
Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp Thr 20 25 30Gly Ile
Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser Phe 35 40 45Val
Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr Gln 50 55
60Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly65
70 75 80Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala
Ser 85 90 95Gly Ser Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp
Ala Ala 100 105 110Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly
Ser Ser Ala Gly 115 120 125Ser Ala Thr Met Glu Gln Ala Val Asn Gln
Ala Thr Ala Ser Gly Val 130 135 140Leu Val Val Ala Ala Ser Gly Asn
Ser Gly Ala Gly Asn Val Gly Phe145 150 155 160Pro Ala Arg Tyr Ala
Asn Ala Met Ala Val Gly Ala Thr Asp Gln Asn 165 170 175Asn Asn Arg
Ala Thr Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile Val 180 185 190Ala
Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr Ala 195 200
205Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val Ala
210 215 220Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln
Ile Arg225 230 235 240Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly
Asn Thr Thr Gln Phe 245 250 255Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Thr Arg 260 26558269PRTBacillus 58Asn Gln Val Thr Pro Trp Gly
Ile Thr Arg Val Gln Ala Pro Thr Ala1 5 10 15Trp Thr Arg Gly Tyr Thr
Gly Thr Gly Val Arg Val Ala Val Leu Asp 20 25 30Thr Gly Ile Ser Thr
His Pro Asp Leu Asn Ile Arg Gly Gly Val Ser 35 40 45Phe Val Pro Gly
Glu Pro Ser Tyr Gln Asp Gly Asn Gly His Gly Thr 50 55 60His Val Ala
Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Val65 70 75 80Gly
Val Ala Pro Asn Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90
95Asn Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Gln Trp Thr
100 105 110Ala Gln Asn Asn Ile His Val Ala Asn Leu Ser Leu Gly Ser
Pro Val 115 120 125Gly Ser Gln Thr Leu Glu Leu Ala Val Asn Gln Ala
Thr Asn Ala Gly 130 135 140Val Leu Val Val Ala Ala Thr Gly Asn Asn
Gly Ser Gly Thr Val Ser145 150 155 160Tyr Pro Ala Arg Tyr Ala Asn
Ala Leu Ala Val Gly Ala Thr Asp Gln 165 170 175Asn Asn Asn Arg Ala
Ser Phe Ser Gln Tyr Gly Thr Gly Leu Asn Ile 180 185 190Val Ala Pro
Gly Val Gly Ile Gln Ser Thr Tyr Pro Gly Asn Arg Tyr 195 200 205Ala
Ser Leu Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 210 215
220Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Thr Gln
Ile225 230 235 240Arg Gln His Leu Thr Ser Thr Ala Thr Ser Leu Gly
Asn Ser Asn Gln 245 250 255Phe Gly Ser Gly Leu Val Asn Ala Glu Ala
Ala Thr Arg 260 265599PRTBacillus 59Val Gly Val Tyr Gly Asp Thr Ser
Ala1 5606PRTBacillus 60Leu Gln Cys Val Gly Ser1 5619PRTBacillus
61Lys Arg Phe Ala Asn Thr Glu Leu Ala1 5629PRTBacillus 62Leu Asp
Gln Ile Phe Phe Thr Arg Trp1 5639PRTBacillus 63Phe Asn Asp Ala Phe
Phe Val Lys Met1 56410PRTBacillus 64Ala Asn Ile Pro Ile Trp Ser Arg
Ser Ala1 5 10659PRTBacillus 65Arg Gln Ser Thr Asp Phe Gly Thr Thr1
5669PRTBacillus 66Val Gln Val Tyr Gly Asp Thr Ser Ala1
5679PRTBacillus 67Arg Arg Phe Ser Asn Ala Thr Arg Ala1
56812PRTBacillus 68Cys Thr Ala Arg Leu Arg Ala Gly Asn Ala Cys Gly1
5 10699PRTBacillus 69Leu Asp Gln Ile Phe Phe Thr Arg Trp1
5709PRTBacillus 70Glu Gln Ile Phe Phe Thr Ser Gly Leu1
5719PRTBacillus 71Gly Arg Phe Ser Asn Ser Lys Phe Lys1
5726PRTBacillus 72Ala Val Leu Arg Asp Cys1 5736PRTBacillus 73Leu
Gln Cys Val Gly Ser1 5749PRTBacillus 74Leu Arg Gln Cys Asn Glu Arg
Cys Val1 57515PRTBacillus 75Ser Pro Val Thr Lys Arg Ala Ser Leu Lys
Ile Asp Ser Lys Lys1 5 10 15769PRTBacillus 76Arg Gln Ser Thr Asp
Phe Gly Thr Thr1 5779PRTBacillus 77Phe Cys Thr Asn Asn Cys Glu Leu
Ser1 5789PRTBacillus 78Asp Phe His Val Lys Tyr Ala Ala Gln1
57914PRTBacillus 79Val Ala Gln Tyr Lys Ala Leu Pro Val Val Leu Glu
Asn Ala1 5 10806PRTBacillus 80Ala Ala Tyr Pro Asp Val1
5819PRTBacillus 81Glu Gln Ile Phe Phe Thr Ser Gly Leu1
5825PRTBacillus 82Val Asp Ala Ala Phe1 5836PRTBacillus 83Ala Val
Leu Arg Asp Cys1 5849PRTBacillus 84Arg Ala Phe Arg Arg Asn Ala Asn
Trp1 58512PRTBacillus 85Cys Thr Ala Arg Leu Arg Ala Gly Asn Ala Cys
Gly1 5 10869PRTBacillus 86Thr Phe His Asp Ala Pro Ala Leu Gln1
58712PRTBacillus 87Cys Thr Ala Arg Val Val Ala Leu Gly Val Cys Gly1
5 10889PRTBacillus 88Gly Arg Phe Ser Asn Ser Lys Phe Lys1
5899PRTBacillus 89Arg Arg Phe Ala Asn Asp His Thr Arg1
5909PRTBacillus 90Lys Arg Phe Ala Asn Thr Glu Pro Ala1
5916PRTBacillus 91Tyr Lys Val Ser Ala Leu1 5926PRTBacillus 92Thr
Gly Lys Tyr Val Ser1 5939PRTThermomyces 93Gln Arg Pro Pro Arg Tyr
Glu Leu Glu1 5949PRTThermomyces 94Glu Leu Glu Tyr Arg Pro Pro Arg
Gln1 5959PRTThermomyces 95His Glu Tyr Asp Met Arg Val Ala Trp1
5969PRTThermomyces 96His Glu Tyr Pro Met Asp Phe His Leu1
5979PRTThermomyces 97Ser Glu Tyr Ser Met Ser Ile Thr Pro1
59812PRTThermomyces 98Cys Val Trp Pro Ala His Ala Pro Leu Ser Cys
Gly1 5 109912PRTThermomyces 99Cys Ser Trp Pro Ser Pro Ala Pro Leu
Ser Cys Gly1 5 1010012PRTThermomyces 100Cys Asp Phe Pro Leu His Ala
Pro Leu Ser Cys Gly1 5 1010112PRTThermomyces 101Cys Leu Phe Pro Ser
Pro Ala Pro Arg Ser Cys Gly1 5 1010212PRTThermomyces 102Cys Asp Gly
Pro Ala Pro Ala Pro Trp Ser Cys Gly1 5 1010312PRTThermomyces 103Cys
Ser Phe Pro Leu Pro Ala Pro Arg Ser Cys Gly1 5
1010412PRTThermomyces 104Cys Val Tyr Pro Ser Pro Ala Pro Trp Ser
Cys Gly1 5 101059PRTThermomyces 105Pro Glu Tyr Thr Met Asn Ala Leu
Ser1 510612PRTThermomyces 106Cys Ser Arg Ser Ala Lys Gly Ala Arg
Leu Cys Gly1 5 101079PRTThermomyces 107Leu Glu Tyr Pro Met Ser Ala
Ser Gln1 51089PRTThermomyces 108Arg Lys Leu Thr Leu Ser Gly Arg
Ser1 51099PRTThermomyces 109Arg Lys Leu Thr Leu Ser Gly Arg Ser1
511010PRTThermomyces 110Ser Tyr Gly Ala Pro Ala Thr Pro Ala Ala1 5
1011110PRTThermomyces 111Pro Ala Ala Gly Tyr Thr Pro Ala Ala Pro1 5
101125PRTThermomyces 112Tyr Lys Leu Ala Tyr1 511310PRTThermomyces
113Lys Tyr Asp Asp Tyr Val Ala Thr Leu Ser1 5 1011410PRTThermomyces
114Glu Val Lys Ala Thr Pro Ala Gly Glu Leu1 5 1011513PRTThermomyces
115Cys Gly Tyr Ser Asn Ala Gln Gly Val Asp Tyr Trp Ile1 5
1011614PRTThermomyces 116Val Pro Gly Ile Asp Pro Asn Ala Cys His
Tyr Met Lys Cys1 5 1011715PRTThermomyces 117Ser Pro Val Thr Lys Arg
Ala Ser Leu Lys Ile Asp Ser Lys Lys1 5 10 1511813PRTThermomyces
118Ile Met Ser Ala Leu Ala Met Val Tyr Leu Gly Ala Lys1 5
101196PRTThermomyces 119Glu Leu Gly Val Arg Glu1
51206PRTThermomyces 120Gly Cys Arg Lys Glu Val1 51216PRTThermomyces
121Leu Arg Ser Val Tyr Gln1 51226PRTThermomyces 122Ser Gly Pro Trp
Ser Trp1 51239PRTBacillus 123Ala Arg Ile Asp Pro Arg Gly Pro Ser1
51249PRTBacillus 124Ala Arg Ile Asp Pro Arg His Gly Ser1
512512PRTBacillus 125Cys Ser Val Ala Lys Ile Asp Pro Arg Thr Cys
Gly1 5 101269PRTBacillus 126Ala Lys Ile Asp Pro Lys Pro Asp Thr1
51279PRTBacillus 127Ala Arg Ile Asp Pro Arg His Gly Ser1
51289PRTBacillus 128Gln Ile Tyr Asn Asp Thr Gly Pro Thr1
512912PRTBacillus 129Cys Gly Ser Ala Thr Ile Asp Pro Arg Gln Cys
Gly1 5 1013012PRTBacillus 130Cys Asn Ala Asp Asn Gln Met Tyr Pro
Gln Cys Gly1 5 101319PRTBacillus 131Ala Arg Ile Asp Pro Arg Gly Pro
Ser1 513212PRTBacillus 132Cys Gly Ser Ala Thr Ile Asp Pro Arg Gln
Cys Gly1 5 1013312PRTBacillus 133Cys Asp Ala Asp Ser Ser Gly Tyr
Pro Leu Cys Gly1 5 101349PRTBacillus 134Gln Leu Tyr Gly Asp Glu Gln
Leu Pro1 51359PRTBacillus 135Arg Tyr Ala Gln Ile Asp Pro Arg Trp1
51369PRTBacillus 136Gly Glu Phe Asn Leu Gly Arg Ser Ser1
513712PRTBacillus 137Cys Asn Ala Asp Ser Trp Gly Tyr Pro Arg Cys
Gly1 5 1013812PRTBacillus 138Cys Asn Ala Asp Asn Gln Met Tyr Pro
Gln Cys Gly1 5 101399PRTBacillus 139Gly Glu Phe Asn Leu Gly Arg Ser
Ser1 514012PRTHumicola 140Cys Val His Ala Gly Pro Arg Ala Gly Thr
Cys Gly1 5 1014112PRTHumicola 141Cys Leu Ser Gly Pro Leu Ala Gly
Arg Val Cys Gly1 5 1014212PRTHumicola 142Cys Arg Ile Ser Pro Trp
Tyr Ser Val Pro Cys Gly1 5 1014312PRTHumicola 143Cys Leu Ser Gly
Pro Ala Ala Gly Gln Ser Cys Gly1 5 1014412PRTHumicola 144Cys Ile
Thr Arg Gly Thr Arg Ala Gly Trp Cys Gly1 5 1014512PRTHumicola
145Cys Leu Ser Gly Pro Leu Ala Gly Arg Val Cys Gly1 5
1014612PRTHumicola 146Cys Leu Thr Ala Gly Pro Ser Ala Gly Tyr Cys
Gly1 5 1014712PRTHumicola 147Cys Tyr Thr Thr Gly Arg Leu Ala Gly
Leu Cys Gly1 5 1014812PRTHumicola 148Cys Val His Ser Gly Pro Arg
Ala Gly Tyr Cys Gly1 5 1014912PRTHumicola 149Cys Val His Ala Gly
Pro Arg Ala Gly Thr Cys Gly1 5 1015012PRTHumicola 150Cys Val His
Ser Gly Leu Ser Arg Arg Leu Leu Arg1 5 1015112PRTHumicola 151Cys
Val Thr Arg Gly Pro Asn Ala Gly Ser Cys Gly1 5 1015212PRTHumicola
152Cys Leu Thr Ala Gly Pro Ser Ala Gly Tyr Cys Gly1 5
1015312PRTHumicola 153Cys Ile Thr Ser Gly Pro Arg Ala Gly Asn Cys
Gly1 5 101549PRTMyceliophthora 154Pro Gln Ser Asp Pro Gly Glu Ser
Gln1 51559PRTMyceliophthora 155Trp Pro Lys Ser Asp Ala Gly Asp Ser1
51569PRTMyceliophthora 156Pro Gln Ser Asp Ala Gly Val Val Met1
51579PRTMyceliophthora 157Asp Pro Val Arg Asp Thr Gly Ala Gly1
51589PRTMyceliophthora 158Gly Pro Ser Arg Asp Ala Gly Leu Leu1
51599PRTMyceliophthora 159Pro Ala Ser Asp Ala Gly Arg Gly Pro1
51609PRTMyceliophthora 160Pro Arg Asp Ser Thr Gly Leu Ala Leu1
51619PRTMyceliophthora 161Pro Gln Ser Asp Pro Gly Glu Ser Gln1
51629PRTMyceliophthora 162Arg Tyr Pro Phe Leu Arg Ala Thr Asn1
51639PRTMyceliophthora 163Gly Ala Ala Arg Asp Ala Arg Ser Ala1
51649PRTMyceliophthora 164Pro Arg Ser Asp Thr Gly Phe Gly Ser1
51659PRTMyceliophthora 165Leu Pro Arg Ser Asp Pro Gly Gly Arg1
51669PRTMyceliophthora 166Asp Pro Ala Arg Asp Thr Gly Asp Val1
51679PRTMyceliophthora 167Ala Pro Lys Ser Asp Asn Gly Ile Thr1
51689PRTMyceliophthora 168Pro Lys Ser Asp Pro Gly Thr Asn Trp1
51699PRTMyceliophthora 169Pro Arg Thr Asp Pro Gly Trp Leu Ala1
51709PRTMyceliophthora 170Leu Pro Arg Ser Asp Pro Gly Gly Arg1
51719PRTMyceliophthora 171Pro Ser Ser Asp Pro Gly Ala Arg Ser1
51729PRTMyceliophthora 172His Val Phe Asp Lys Asn Val Thr Arg1
51739PRTMyceliophthora 173Pro Arg Ser Asp Pro Gly Thr Pro Thr1
51749PRTMyceliophthora 174Pro Arg Asp Ser Thr Gly Leu Ala Leu1
51759PRTMyceliophthora 175Pro Arg Asp Ser Thr Gly Leu Ala Leu1
51769PRTMyceliophthora 176Pro Ser Ser Asp Pro Gly Ala Arg Ser1
51779PRTMyceliophthora 177Pro Lys Ser Asp Pro Gly Thr Asn Trp1
51789PRTMyceliophthora 178Trp Pro Lys Ser Asp Ala Gly Asp Ser1
51799PRTMyceliophthora 179Pro Gln Ser Asp Ala Gly Val Val Met1
51809PRTMyceliophthora 180Gly Pro Ser Arg Asp Ala Gly Leu Leu1
51819PRTMyceliophthora 181Pro Ala Ser Asp Ala Gly Arg Gly Pro1
51829PRTMyceliophthora 182Ala Pro Lys Ser Asp Asn Gly Ile Thr1
51839PRTMyceliophthora 183Trp Pro Lys Ser Asp Ala Gly Asp Ser1
51849PRTMyceliophthora 184Pro Gln Ser Asp Ala Gly Val Val Met1
51859PRTMyceliophthora 185Gly Pro Ser Arg Asp Ala Gly Leu Leu1
51869PRTMyceliophthora 186Pro Ala Ser Asp Ala Gly Arg Gly Pro1
51879PRTMyceliophthora 187Ala Pro Lys Ser Asp Asn Gly Ile Thr1
51889PRTMyceliophthora 188Asp Pro Val Arg Asp Thr Gly Ala Gly1
51899PRTMyceliophthora 189Pro Arg Ser Asp Thr Gly Phe Gly Ser1
51909PRTMyceliophthora 190Asp Pro Ala Arg Asp Thr Gly Asp Val1
51919PRTMyceliophthora 191Asp Pro Val Arg Asp Thr Gly Ala Gly1
51929PRTMyceliophthora 192Pro Arg Ser Asp Thr Gly Phe Gly Ser1
51939PRTMyceliophthora 193Asp Pro Ala Arg Asp Thr Gly Asp Val1
51949PRTMyceliophthora 194Asp Pro Val Arg Asp Thr Gly Ala Gly1
51959PRTMyceliophthora 195Pro Arg Ser Asp Thr Gly Phe Gly Ser1
51969PRTMyceliophthora 196Asp Pro Ala Arg Asp Thr Gly Asp Val1
51975PRTBetula 197Ala Leu Pro Gln Ser1 51984PRTBetula 198Ala Gly
Ile Leu11995PRTBetula 199Ala Asn Arg Thr Val1 52005PRTBetula 200Gly
Ile Leu Val Tyr1 52014PRTBetula 201Ala Ser Thr Arg12026PRTBetula
202Arg Asn Ala Phe Leu Ser1 52035PRTBetula 203Lys Arg Gln Ser Ala1
52044PRTBetula 204Ser Thr Arg Cys12056PRTBetula 205Asn Arg Gly Leu
Thr Val1
52064PRTBetula 206Ser Thr Ala Asn12074PRTBetula 207Ala Ile Leu
Val12085PRTBetula 208Glu Asn Arg Ser Val1 52094PRTBetula 209Asp Gly
Asn Thr12104PRTBetula 210Asp Glu Cys Thr12115PRTBetula 211Phe Trp
Tyr Gly Leu1 52124PRTBetula 212Leu Arg Trp Ala12134PRTBetula 213Arg
Lys Gln Thr12144PRTBetula 214Gln Arg Ser Trp12155PRTBetula 215Val
Leu Ser Phe Asn1 52166PRTBetula 216Ala Glu His Asn Pro Thr1
52175PRTBetula 217Ala Gly Leu Lys Met1 52184PRTBetula 218Glu Asp
Lys Trp12198PRTBetula 219Ala Cys Leu Pro Thr Val Trp Tyr1
52205PRTBetula 220Ala Ser Leu Pro Met1 52214PRTBetula 221Ser Tyr
Leu Asn12226PRTBetula 222Ala Glu Leu Phe Pro Arg1 52234PRTBetula
223Thr Ser Phe Arg12244PRTBetula 224Ala Pro Ser Gly12256PRTBetula
225Cys Phe Ile Lys Leu Trp1 52265PRTBetula 226Phe Ile Lys Leu Trp1
52275PRTBetula 227Ala Gly Ile Leu Val1 52287PRTBetula 228Lys Arg
Gln His Asn Gly Pro1 52294PRTBetula 229Gly Ile Leu
Val12306PRTBetula 230Ile Lys Leu Pro Gln Ser1 52314PRTBetula 231Leu
Ile Met Asn12324PRTBetula 232Thr Tyr Ala Pro12334PRTBetula 233Ile
Leu Val Ser12345PRTBetula 234His Asn Gln Gly Cys1 52355PRTBetula
235Ala Val Leu Cys Tyr1 52364PRTBetula 236Leu Phe Gln
Ala12376PRTBetula 237Ala Ile Leu Met Asn Val1 52386PRTBetula 238Ala
Gly Ser Tyr Leu Glu1 52396PRTBetula 239Leu Ile Ala Gly Val Ser1
52404PRTBetula 240Lys His Gln Asp12414PRTBetula 241Ser His Gln
Glu12427PRTBetula 242Ser Thr Ala Pro Leu Trp Val1 52437PRTBetula
243Thr Ser Lys His Arg Gln Gly1 52446PRTBetula 244Leu Ile Arg Lys
Gly Pro1 52458PRTBetula 245Asp Ser Arg Thr Gln Gly Lys His1
52466PRTBetula 246Asp Glu Lys Gln His Thr1 52475PRTBetula 247Arg
Lys Gln Asp Thr1 52484PRTBetula 248Ser Thr Arg Glu1
* * * * *
References