U.S. patent application number 12/578155 was filed with the patent office on 2010-03-18 for method of selectively introducing amino substituent.
This patent application is currently assigned to DAIICHI PHARMACEUTICAL CO., LTD.. Invention is credited to YUTAKA KITAGAWA, MAKOTO MUTO.
Application Number | 20100069435 12/578155 |
Document ID | / |
Family ID | 33534813 |
Filed Date | 2010-03-18 |
United States Patent
Application |
20100069435 |
Kind Code |
A1 |
MUTO; MAKOTO ; et
al. |
March 18, 2010 |
METHOD OF SELECTIVELY INTRODUCING AMINO SUBSTITUENT
Abstract
Provided are a technique for position-selectively introducing an
amino group into a difluorobenzoic acid, a novel process for
producing a quinolonecarboxylic acid derivative serving as an
antibacterial agent, and intermediates in the production. The
process comprises treating a compound represented by formula (6):
##STR00001## with a base in a water-containing solvent. The
intermediates are represented by formula (6): ##STR00002## formula
(5): ##STR00003## formula (4): ##STR00004## or formula (3):
##STR00005##
Inventors: |
MUTO; MAKOTO; (TOKYO,
JP) ; KITAGAWA; YUTAKA; (TOKYO, JP) |
Correspondence
Address: |
OBLON, SPIVAK, MCCLELLAND MAIER & NEUSTADT, L.L.P.
1940 DUKE STREET
ALEXANDRIA
VA
22314
US
|
Assignee: |
DAIICHI PHARMACEUTICAL CO.,
LTD.
TOKYO
JP
|
Family ID: |
33534813 |
Appl. No.: |
12/578155 |
Filed: |
October 13, 2009 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10560823 |
Dec 15, 2005 |
7626029 |
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PCT/JP04/08607 |
Jun 18, 2004 |
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12578155 |
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Current U.S.
Class: |
514/312 ;
546/156; 548/566 |
Current CPC
Class: |
A61P 31/04 20180101;
C07D 401/04 20130101 |
Class at
Publication: |
514/312 ;
548/566; 546/156 |
International
Class: |
A61K 31/4709 20060101
A61K031/4709; C07D 207/04 20060101 C07D207/04; C07D 401/02 20060101
C07D401/02 |
Foreign Application Data
Date |
Code |
Application Number |
Jun 19, 2003 |
JP |
2003-175212 |
Claims
1-12. (canceled)
13. A compound represented by formula (3), (4), (5) or (6), wherein
formula (3) is: ##STR00018## wherein R and R.sup.1 represent each
independently a lower alkyl group and A represents an
amino-protecting group; wherein formula (4) is: ##STR00019##
wherein R.sup.1 represents a lower alkyl group and A represents an
amino-protecting group; wherein formula (5) is: ##STR00020##
wherein R.sup.1 and R.sup.2 represent each independently a lower
alkyl group and A represents an amino-protecting group; and wherein
formula (6) is: ##STR00021## wherein R.sup.1 and R.sup.2 represent
each independently a lower alkyl group and A represents an
amino-protecting group.
14. The compound of claim 13, which is the compound of formula
(3).
15. The compound of claim 13, which is the compound of formula
(4).
16. The compound of claim 13, which is the compound of formula
(5).
17. The compound of claim 13, which is the compound of formula
(6).
18. A method for making the compound of formula (3) of claim 13
comprising: reacting a compound represented by formula (2) in a
solvent which dissolves the compound of formula (2): ##STR00022##
wherein R represents a lower alkyl group and R.sup.1 represents a
lower alkyl group; with a compound represented by the following
formula: ##STR00023## wherein A represents an amino-protecting
group.
19. A method for making the compound of formula (4) of claim 13
comprising: hydrolyzing a compound of formula (3), where formula
(3) is: ##STR00024## wherein R represents a lower alkyl group,
R.sup.1 represents a lower alkyl group, and A represents an
amino-protecting group.
20. A method for making the compound of formula (5) from a compound
of formula (4) of claim 13 comprising: converting the compound of
formula (4) to an acid halide with a halogenating agent or to an
acylimidazole with a carbonyldiimidazole condensing agent, and then
reacting the resultant product with a magnesium salt of a lower
alkyl monoester of malonic acid; or, converting the compound of
formula (4) to an acid halide in the presence of a base, with a
lower alkyl monoester of malonic acid; where formula (4) is:
##STR00025## wherein R.sup.1 represents a lower alkyl group, and A
represents an amino-protecting group.
21. A method for making the compound of formula (6) from a compound
of formula (5) of claim 13 comprising: reacting the compound of
formula (5) with an ortho ester and then with
(1R,2S)-2-fluorocyclopropylamine or a salt thereof; where the
compound of formula (5) is: ##STR00026## wherein R.sup.1 represents
a lower alkyl group, A represents an amino-protecting group, and
R.sup.2 represents a lower alkyl group.
22. A compound of formula (I): ##STR00027## wherein R.sup.1
represents a lower alkyl group and A represents an amino-protecting
group.
23. A composition comprising the compound of claim 22 in an amount
sufficient to exert an antibacterial effect.
24. An agricultural chemical or veterinary drug comprising the
compound of claim 22.
25. A method for treating a bacterial infection comprising
administering the compound of claim 22 to a subject in need
thereof.
26. The method of claim 25, wherein said bacterial infection is
caused by gram-positive bacteria.
Description
TECHNICAL FIELD
[0001] The present invention relates to a method for producing a
quinolonecarboxylic acid which is a synthetic antibacterial agent
and is highly promising as an excellent medicament, an agricultural
chemical or a veterinary drug, as well as novel intermediates in
the production thereof.
BACKGROUND ART
[0002] Quinolonecarboxylic acid derivatives are widely used for
medical purpose as a synthetic antibacterial agent. However,
emergence of resistant bacteria represented by MRSA has become a
major obstacle in such treatment. The quinolonecarboxylic acid
derivative represented by formula (1'):
##STR00006##
not only exhibits excellent effects on MRSA but also exhibits an
antibacterial activity against resistant gram-positive bacteria,
and thus is a compound which can solve problems associated with
various resistant bacteria. As a process for producing the compound
is known a process shown by the following reaction scheme (for
example, see Patent Document 1):
##STR00007## ##STR00008##
Patent Document 1: International Publication WO 02/040478
gazette
DISCLOSURE OF THE INVENTION
Problems to be Solved by the Invention
[0003] Hitherto, there has not been a technique for
position-selectively introducing an amino group into
difluorobenzoic acids. Thus it has been conducted to introduce an
amino group into a quinolone mother nucleus at 7-position after
producing the mother nucleus. According to a conventional process,
however, expensive (1R,2S)-2-fluorocyclopropylamine tosylate is
used at an earlier step in the production of the mother nucleus,
thereby providing a problem of high production cost.
[0004] Accordingly, an object of the present invention is to
develop a technique for position-selectively introducing an amino
group into difluorobenzoic acids to provide a novel process for
producing a quinolone and intermediates produced in the
process.
Means to Solve the Problem
[0005] The present inventors have made extensive research and have
found that it is possible to position-selectively control the
reaction of introducing an amino substituent, particularly a cyclic
amino substituent, into a 2,4-difluoro-3-alkoxybenzoate by
selecting a reaction solvent. For the production of a compound
which is necessary for producing a quinolone compound, in which an
amino substituent is introduced at 4-position, such as a compound
of formula (3) shown below, use of dimethylsulfoxide as a reaction
solvent is most effective. By producing selectively a compound in
which an amino substituent is introduced at 4-position and using
the compound as a key intermediate, a novel process for producing a
quinolone has been completed. An example of the process of the
present invention is shown by way of the following reaction
scheme:
##STR00009## ##STR00010##
wherein, R, R.sup.1 and R.sup.2 represent each independently a
lower alkyl group, and A represents an amino-protecting group.
[0006] Thus, according to the present invention, there is provided
a process for producing a compound represented by formula (1):
##STR00011##
wherein R.sup.1 represents a lower alkyl group and A represents an
amino-protecting group which comprises treating a compound
represented by formula (6):
##STR00012##
wherein R.sup.1 and A are as defined above and R.sup.2 represents a
lower alkyl group with a base in a water-containing solvent.
[0007] The compound represented by formula (6) can be prepared by
reacting a compound represented by formula (5):
##STR00013##
wherein R.sup.1, R.sup.2 and A are as defined above with an ortho
ester and then with (1R,2S)-2-fluorocyclopropylamine or a salt
thereof.
[0008] The compound represented by formula (5) can be prepared by
converting a compound represented by formula (4):
##STR00014##
wherein R.sup.1 and A are as defined above to an acid halide with a
halogenating agent or to an acylimidazole with a
carbonyldiimidazole condensing agent, and then reacting the
resultant product with a magnesium salt of a lower alkyl monoester
of malonic acid, or, in the presence of a base, with a lower alkyl
monoester of malonic acid.
[0009] The compound represented by formula (4) can be prepared by
hydrolyzing a compound represented by formula (3):
##STR00015##
wherein R represents a lower alkyl group and R.sup.1 and A are as
defined above.
[0010] The compound represented by formula (3) can be prepared by
reacting a compound represented by formula (2):
##STR00016##
wherein R and R.sup.1 are as defined above with a
(3R)-3-(1-aminocyclopropyl)-pyrrolidine represented by the
following formula:
##STR00017##
wherein A is as defined above in a solvent which dissolves the
compound of formula (2).
[0011] The compound represented by formula (2) may be prepared by
converting a 2,4-difluoro-3-alkoxybenzoic acid to an acid halide
thereof, particularly an acid chloride thereof, by using a
halogenating agent such as thionyl chloride or oxalyl chloride, and
then adding thereto an alkyl alcohol. In addition, it is possible
to obtain the compound by a method usually conducted in which an
acid catalyst is used in an alcohol.
EFFECTS OF THE INVENTION
[0012] According to the process of the invention, an amino group is
position-selectively introduced into a difluorobenzoic acid,
whereby expensive (1R,2S)-2-fluorocyclopropylamine tosylate is not
necessary at the earlier step of the process for the production of
the mother nucleus and thus the production cost is reduced.
MODE FOR CARRYING OUT THE INVENTION
[0013] R.sup.1 in formulae (1) through (6) is preferably a straight
or branched lower alkyl group having 1 to 3 carbon atoms such as
methyl, ethyl, n-propyl or isopropyl, and is particularly
preferably methyl.
[0014] R.sup.2 in formulae (5) and (6) is preferably a straight or
branched lower alkyl group having 1 to 6 carbon atoms such as
methyl, ethyl, n-propyl, isopropyl, isopentyl or neopentyl, more
preferably methyl or ethyl, and particularly preferably ethyl.
[0015] R in formulae (2) and (3) is preferably a straight or
branched lower alkyl group having 1 to 6 carbon atoms such as
methyl, ethyl, n-propyl, isopropyl, isopentyl or neopentyl, and
more preferably methyl or ethyl.
[0016] Examples of the amino-protecting group represented by A in
formulae (1), (3), (4), (5) and (6) include alkoxycarbonyl groups,
aralkyloxycarbonyl groups, acyl groups, aralkyl groups, alkyl
groups and substituted silyl groups. Preferred amino-protecting
groups include alkoxycarbonyl groups having 2 to 5 carbon atoms and
aralkyloxycarbonyl groups, and particularly preferred
amino-protecting groups are tert-butoxycarbonyl(Boc) group and
benzyloxycarbonyl(Cbz) group.
[0017] The compounds of formula (6) are novel compounds.
Particularly preferred compounds of formula (6) include one wherein
R.sup.1 is methyl, R.sup.2 is ethyl and A is
tert-butoxycarbonyl(Boc).
[0018] The compounds of formula (5) are novel compounds.
Particularly preferred compounds of formula (5) include one wherein
R.sup.1 is methyl, R.sup.2 is ethyl and A is
tert-butoxycarbonyl(Boc).
[0019] The compounds of formula (4) are novel compounds.
Particularly preferred compounds of formula (4) include one wherein
R.sup.1 is methyl and A is tert-butoxycarbonyl(Boc).
[0020] The compounds of formula (3) are novel compounds.
Particularly preferred compounds of formula (3) include those
wherein R.sup.1 is methyl, R is methyl or ethyl and A is
tert-butoxycarbonyl(Boc).
[0021] Reaction steps involved in the production of compound (1)
which is a precursor of the quinolonecarboxylic acid antibacterial
agent starting from a substituted benzoic acid is described in
detail.
Substituted Benzoic Acid.fwdarw.Compound (2)
[0022] Compound (2) may be prepared by converting a
2,4-difluoro-3-alkoxybenzoic acid to an acid halide, particularly
an acid chloride, by using a halogenating agent such as thionyl
chloride or oxalyl chloride, and then adding thereto an alkyl
alcohol (ROH). Exemplary solvents which may be used in the
conversion to the acid halide include ether compounds such as
tetrahydrofuran, diethyl ether, dioxane and dimethoxyethane;
aromatic compounds such as benzene, toluene and xylene; chlorinated
compounds such as methylene chloride and chloroform; ester
compounds such as methyl acetate and ethyl acetate; and nitrile
compounds such as acetonitrile. The reaction is accomplished at a
temperature in the range of -30 to 170.degree. C., preferably 0 to
110.degree. C. The alkyl alcohol to be added may be a primary or
secondary alcohol and is particularly preferably ethanol. After
completion of the reaction, the product may be obtained according
to a commonly used method by adding the reaction mixture which has
been optionally concentrated to water, removing the resulting
salts, and extracting the filtrate with a water-insoluble organic
solvent. The target product can be obtained in a high purity by
removing the solvent from the extract. However, when further
purification is required, a pure product may be isolated by using
column chromatography. The other method for preparing compound (2)
includes a usual method which is conducted in the presence of an
acid catalyst in an alcohol.
Compound (2).fwdarw.Compound (3)
[0023] Compound (3) may be prepared by reacting compound (2) with 1
to 2 times moles of an amino-protected
(3R)-3-(1-aminocyclopropyl)-pyrrolidine, particularly at a
stoichiometric ratio (molar ratio) of about 1:1. In this reaction
with the pyrrolidine, substitution reaction occurs at
ortho-position (2-position) or para-position (4-position) relative
to the carboxyl group. Surprisingly, it was found by the present
inventors that the position of compound (2) at which the
pyrrolidine reacts varies depending on the solvent used and that
different compounds having different reaction sites can be
selectively obtained by choosing the solvent.
[0024] The solvent which may be used in this substitution reaction
includes those which dissolve compound (2), such as, for example,
ether compounds such as tetrahydrofuran, diethyl ether, dioxane and
dimethoxyethan; aromatic compounds such as benzene, toluene, and
xylene; chlorinated compounds such as methylene chloride and
chloroform; ester compounds such as methyl acetate and ethyl
acetate; nitrile compounds such as acetonitrile; N,N-dimethyl
acetoformamide, dimethylsulfoxide and ionic liquids. In this
process, the polarity of the solvent used is important. In order to
effectively obtain compound (3) which is substituted at 4-position,
it was revealed that use of a highly polar solvent such as
dimethylsulfoxide, N,N-dimethylformamide or an ionic liquid is
preferred. Among these highly polar solvents, dimethylsulfoxide is
particularly preferred.
[0025] The reaction temperature may be in the range of 0 to
170.degree. C., preferably 0 to 100.degree. C.
[0026] The reaction is preferably conducted in the presence of a
base. The base to be added may be either organic or inorganic, and
is preferably a tertiary amine. The amine may be either aromatic or
non-aromatic, and is preferably a trialkylamine. Triethylamine may
be used. The base is suitably used in an amount of 1 to 5
equivalents, preferably 1 to 2 equivalents based on the amount of
compound (2).
[0027] After completion of the reaction, the product may be
obtained according to a commonly used method by adding the reaction
mixture which has been optionally concentrated to water, removing
the resulting inorganic salts, and extracting the filtrate with a
water-insoluble organic solvent. The target product can be obtained
in a high purity by removing the solvent from the extract. However,
when further purification is required, a pure product may be
isolated by using column chromatography or recrystallization.
[0028] This selective amino-substituent introduction reaction found
by the present inventors is believed to proceed similarly in the
reaction using such cyclic amine compound used in the constitution
(introduction) of an amino-substituent into known quinolone
compounds. Such cyclic amine compounds include, for example,
5-membered or 6-membered ring cyclic amino compounds and bicyclic
amino compounds. Specifically, the cyclic amine compounds include
pyrrolidine, 3-aminopyrrolidine, 2-methyl-4-aminopyrrolidine,
3-amino-4-methylpyrrolidine, 7-amino-5-azaspiro[2.4]heptane,
1-amino-5-azaspiro[2.4]heptane, 1-amino-3-azabicyclo[3.1.0]hexane,
3-hydroxypyrrolidine, piperazine, 3-methylpiperazine,
4-methylpiperazine, 3,5-dimethylpiperazine,
2,8-diazabicyclo[4.3.0]nonane and
2-oxa-5,8-azabicyclo[4.3.0]nonane, all of which may be in an
optically active form. When amino group or hydroxyl group is
additionally included as a substituent on the ring, these groups
may be protected.
Compound (3).fwdarw.Compound (4)
[0029] In order to obtain compound (4) from compound (3), the ester
of compound (3) may be hydrolyzed. The hydrolysis of this ester may
be conducted under conditions usually used and the conditions are
not particularly restricted as long as they do not remove the
protecting group of the amino group. The hydrolysis of this process
is preferably conducted under basic conditions. The base which may
be used is preferably an inorganic base and includes sodium
hydroxide and potassium hydroxide. The base is suitably used in an
amount of 1 to 50 equivalents, preferably 1 to equivalents, based
on the amount of compound (3). The reaction may be conducted at a
temperature in the range of from room temperature to 300.degree.
C., preferably room temperature to about 100.degree. C. The
reaction time varies depending on the reaction temperature, and may
be approximately from 1 to 48 hours. Usually the reaction completes
in 1 to 24 hours. After completion of the reaction, the reaction
mixture is acidified and then extracted with a water-insoluble
organic solvent or a solid matter is separated from the reaction
mixture to obtain compound (4). The acid used for the acidification
is not particularly restricted as long as it does not remove
amino-protecting group and is preferably citric acid.
Compound (4).fwdarw.Compound (5)
[0030] As a method for producing compound (5), compound (4) is
reacted with a halogenating agent such as thionyl chloride
preferably at a stoichiometric ratio (molar ratio) of about 1:1 to
convert compound (4) to an acid halide, particularly an acid
chloride or, alternatively, with a condensing agent such as
1,1'-carbonyldiimidazole preferably in an amount of 1 to 2 moles
per mole of compound (4) to convert compound (4) to an
acylimidazole, and then magnesium salt of a lower alkyl monoester
of malonic acid is added to the product in an amount of 1 to 3
moles per mole of compound (4) to effect reaction. The other method
includes a method as disclosed in JP-A-1-100166 wherein a lower
alkyl monoester of malonic acid is reacted in the presence of a
base such as, for example, an alkyl lithium or sodium hydride in
place of the magnesium salt. The term "lower alkyl" herein refers
to an alkyl having 1 to 6, preferably 1 to 3 carbon atoms. The
solvent which may be used in these reactions includes ether
compounds such as tetrahydrofuran, diethyl ether, dioxane and
dimethoxyethan; aromatic compounds such as benzene, toluene, and
xylene; chlorinated compounds such as methylene chloride and
chloroform; ester compounds such as methyl acetate and ethyl
acetate; nitrile compounds such as acetonitrile;
N,N-dimethylacetoformamide, dimethylsulfoxide and ionic liquids.
Among them, ether compounds are particularly preferred. The
reaction temperature is in the range of 0 to 170.degree. C.,
preferably room temperature. After completion of the reaction, the
product may be obtained according to a commonly used method by
adding the reaction mixture which has been optionally concentrated
to water, and extracting the resulting mixture with a
water-insoluble organic solvent. The target product can be obtained
in a high purity by removing the solvent from the extract. However,
when further purification is required, a pure product may be
isolated by using column chromatography.
Compound (5).fwdarw.Compound (6)
[0031] Compound (6) may be prepared by reacting compound (5) with
an ortho ester such as methyl orthoformate or ethyl orthoformate in
acetic anhydride, concentrating the reaction mixture, dissolving
the residue and acting thereon (1R,2S)-2-fluorocyclopropylamine or
a salt thereof, particularly tosylate thereof. The amount of
(1R,2S)-2-fluorocyclopropylamine or the salt thereof used therein
per mole of compound (5) is preferably 1 to 1.5 moles, particularly
at a stoichiometric ratio (molar ratio) of about 1:1. The solvent
which is used therein may be any solvent as long as it does not
interfere with the reaction. The reaction temperature is in the
range of 0 to 170.degree. C., preferably room temperature. After
completion of the reaction, the product may be obtained according
to a commonly used method by adding the reaction mixture which has
been optionally concentrated to water, and extracting the resulting
mixture with a water-insoluble organic solvent. The target product
can be obtained in a high purity by removing the solvent from the
extract. However, when further purification is required, a pure
product may be isolated by using column chromatography.
Compound (6).fwdarw.Compound (1)
[0032] Compound (1) may be obtained by dissolving compound (6) in a
water-miscible solvent and treating the solution with a base in the
presence of water. Under such condition, a ring-closure reaction to
give a quinoline ring and hydrolysis of the ester simultaneously
proceed to provide compound (1). The water-miscible solvent is not
particularly restricted as long as it does not interfere with the
reaction and includes alcohol solvents such as methanol, ethanol
and n-propanol; and ether compounds such as dioxane and
dimethoxyethane. The base which may be used includes inorganic
bases such as sodium hydroxide, potassium hydroxide and lithium
hydroxide. The amount of the solvent used is in the range 1 to 100
times by weight, preferably 3 to 10 times by weight the amount of
compound (6). The amount of the base may be not less than 2 moles
per mole of compound (6). The base is dissolved in water and used
in the form of an aqueous solution. The amount of water is adjusted
so that the base dissolved in water becomes approximately 0.5 to
10N, preferably approximately 2 to 5N. The reaction may be
conducted at a temperature in the range of from room temperature to
300.degree. C., preferably room temperature to 100.degree. C. The
reaction time varies depending on the reaction temperature, and is
approximately from 1 to 48 hours. Usually the reaction completes in
1 to 5 hours. After completion of the reaction, the reaction
mixture is acidified and then extracted with a water-insoluble
organic solvent or a solid matter is separated from the reaction
mixture to obtain compound (1). The acid used for the acidification
is not particularly restricted as long as it does not remove
amino-protecting group and is preferably citric acid.
[0033] Incidentally, the reaction in this process can be carried
out similarly under conditions for a so-called two layer-reaction
using a water-immiscible organic solvent, an aqueous base and a
phase-transfer catalyst.
[0034] By removing the protecting group of the amino group in the
resulting compound (1), the quinolonecarboxylic acid derivative
represented by formula (1') which is useful as an antibacterial
agent is obtained. Deprotection may be carried out under conditions
suitable for the protecting group used, and may be effected, for
example, by hydrolysis using hydrochloric acid or the like.
Incidentally, compound (1') may be isolated as an acid addition
salt or hydrate thereof.
EXAMPLES
[0035] Processes for production of the compounds of the invention
and a quinolonecarboxylic acid derivative using these compounds as
an intermediate are specifically described below.
Reference Example 1
Ethyl 2,4-difluoro-3-methoxybenzoate
[0036] 2,4-Difluoro-3-methoxybenzoic acid (18.8 g) was placed in a
reaction vessel and was suspended in 100 ml of toluene added
thereto at room temperature. After dropwise addition of 0.5 mL of
N,N-dimethylformamide, 14.6 mL of thionyl chloride was added
dropwise, and the resulting mixture was heated to 60.degree. C.
with stirring. After 7 hours, 50 mL of ethanol was added and an
insoluble matter was filtered out. The filtrate was washed with
water and saturated brine, and dried with anhydrous sodium sulfate.
The solvent was distilled away to give the title compound (21.4 g)
as a pale yellow oil.
Example 1
[0037] (3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidine
(64 mg, 85% content) was dissolved in a solvent (0.45 mL, see Table
1 below). After adding thereto ethyl 2,4-difluoro-3-methoxybenzoate
(45 mg) and triethylamine (0.04 mL), the mixture was stirred at
75.degree. C. After 18 hours, the mixture was allowed to cool,
subjected to liquid separation with 10% aqueous citric acid
solution, and extracted with AcOEt. An organic layer was dried with
anhydrous sodium sulfate. After filtration, the solvent was
distilled away. The residue was measured by .sup.1H-NMR to
determine position-selectivity of amino group to an aromatic
compound.
Position-Selectivity of Amino Group
TABLE-US-00001 [0038] Solvent 2-position 4-position
Dimethylsulfoxide 18 82 1-Ethyl-3-methylimidazolium 20 80
trifluorosulfonate N,N-Dimethylformamide 29 71 Acetonitrile 50 50
Tetrahydrofuran 72 28 Ethyl acetate 80 20 Toluene 90 10
Ethyl
2-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-4-
-fluoro-3-methoxybenzoate (a compound introduced at 2-position)
[0039] colorless oil; .sup.1H-NMR (400 MHz, CDCl.sub.3)
.delta.0.73-0.85 (m, 4H), 1.36 (t, J=6.8 Hz, 3H), 1.42 (s, 9H),
1.66-1.72 (m, 1H), 1.92-2.05 (m, 1H), 2.28-2.43 (m, 1H), 3.26-3.34
(m, 3H), 3.47-3.53 (m, 1H), 3.82 (s, 3H), 4.10 (q, J=6.8 Hz, 2H),
4.99 (br s, 1H), 6.63 (t, J=9.0 Hz, 1H), 7.19 (dd, J=9.0, 6.4 Hz,
1H).
Ethyl
4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-
-fluoro-3-methoxybenzoate (a compound introduced at 4-position)
[0040] white crystal; .sup.1H-NMR (400 MHz, CDCl.sub.3)
.delta.0.76-0.88 (m, 4H), 1.37 (t, J=7.3 Hz, 3H), 1.41 (s, 9H),
1.70-1.79 (m, 1H), 1.95-2.05 (m, 1H), 2.28-2.38 (m, 1H), 3.30-3.38
(m, 1H), 3.45-3.60 (m, 3H), 3.78 (s, 3H), 4.33 (q, J=7.3 Hz, 2H),
4.90 (br s, 1H), 6.31 (d, J=9.0 Hz, 1H), 7.19 (t, J=9.0, 1H).
Example 2
Ethyl
4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-
-fluoro-3-methoxybenzoate
[0041] (3R)-3-(1-Tert-butoxycarbonylaminocyclopropyl)-pyrrolidine
(4.75 g, 85% content) was dissolved in dimethylsulfoxide (30 mL).
After adding thereto ethyl 2,4-difluoro-3-methoxybenzoate (3.2 g)
and triethylamine (3.1 mL), the mixture was stirred at 75.degree.
C. After 18 hours, the mixture was allowed to cool, incorporated
with 10% aqueous citric acid solution, and extracted with AcOEt. An
organic layer was dried with anhydrous sodium sulfate and filtered,
and then the solvent was distilled away. The resulting residual
crystals (6.22 g) (2-position:4-position=17:83) were subjected to
chromatography on silica gel (hexane:ethyl acetate=4:1) to give the
title compound as white crystals (4.75 g).
Example 3
4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-fluor-
o-3-methoxybenzoic acid
[0042] Ethyl
4-[(3R)-3-(1-tert.-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-flu-
oro-3-methoxybenzoic acid (3.61 g) was dissolved in 28 mL of
ethanol, 1N aqueous sodium hydroxide solution (34.5 mL) was added
to the solution, and the mixture was heated to 50.degree. C. with
stirring. After completion of the reaction, 10% aqueous citric acid
solution was added to the mixture followed by extraction with
chloroform. An organic layer was dried with anhydrous sodium
sulfate and filtered, and then the solvent was distilled away. The
title compound was obtained as residual crystals (4.45 g). The
crystals were used in the next step without purification.
Example 4
Ethyl
{4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]--
2-fluoro-3-methoxybenzoyl}acetate
[0043] A crude product of
4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-fluo-
ro-3-methoxybenzoic acid (2.0 g) was dissolved in 10 mL of
tetrahydrofuran at room temperature, and 1,1'-carbonyldiimidazole
(0.97 g) was added thereto. The reaction mixture was stirred for 2
hours. In the meantime, 245 mL of tetrahydrofuran was added to
monoethyl malonate (1.59 g) while cooling in a water bath. After
completion of the dropwise addition, magnesium ethoxide (0.69 g)
was added and the reaction mixture was stirred for two hours. After
2 hours, this reaction mixture was added to the former reaction
mixture and the resulting reaction mixture was stirred for
additional two hours. The reaction mixture was extracted with ethyl
acetate after addition of 102 mL of water. An organic layer was
washed with an aqueous sodium hydrogen carbonate solution and a
saturated brine, dried with anhydrous sodium sulfate and filtered.
The filtrate was concentrated under reduced pressure to give 2.6 g
of a residual oil. The resulting residual oil was subjected to
chromatography on silica gel (hexane:ethyl acetate=2:1) to give the
title compound as a pale yellow oil (1.24 g).
[0044] pale yellow oil; .sup.1H-NMR (400 MHz, CDCl.sub.3)
.delta.0.73-0.88 (m, 4H), 1.37 (t, J=6.8 Hz, 3H), 1.41 (s, 9H),
1.66-1.80 (m, 1H), 1.95-2.05 (m, 1H), 2.20-2.44 (m, 1H), 3.25-3.40
(m, 1H), 3.50-3.65 (m, 3H), 3.77 (s, 3H), 3.90 (d, J=3.6 Hz, keto),
4.23 (q, J=6.8 Hz, 2H), 4.92 (br s, 1H), 5.73 (s, enol), 6.31 (d,
J=9.0 Hz, 1H), 7.58 (t, J=9.0, 1H), 12.77 (br s, enol).
Example 5
Ethyl (E and
Z)-2-{4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]--
2-fluoro-3-methoxybenzoyl}-3-[(1R,2S)-2-fluoro-1-cyclopropylamino]acetate
[0045] Ethyl
{4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-2-flu-
oro-3-methoxybenzoyl}acetate (0.79 g) was dissolved in acetic
anhydride (0.48 mL) and ethyl ortoformate (0.71 mL) at room
temperature. The reaction liquid was heated (internal temperature:
120.degree. C.), stirred for 3 hours and then concentrated under
reduced pressure. The concentrated residue was dissolved in toluene
and toluene was distilled away under reduced pressure. The residue
was added eith ethyl acetate (8.0 mL) and was dissolved therein,
followed by addition of (1R,2S)-2-fluorocyclopropylamine tosylate
(0.46 g) and triethylamine (0.36 mL) in a water bath and stirring
for two hours. An insoluble matter in the reaction mixture was
distilled out, and the filtrate was washed with a saturated brine
and water, and dried with anhydrous sodium sulfate. After
filtration, the filtrate was concentrated under reduced pressure to
give a crude product (1.11 g) of the title compound which is a
mixture of E compound and Z compound as a red oil.
Example 6
7-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]-1-[(1R,-
2S)-2-fluoro-1-cyclopropylamino]-1,4-dihydro-8-methoxy-4-oxoquinoline-3-ca-
rboxylic acid
[0046] A crude product of ethyl (E and
Z)-2-{4-[(3R)-3-(1-tert-butoxycarbonylaminocyclopropyl)-pyrrolidin-1-yl]--
2-fluoro-3-methoxybenzoyl}-3-[(1R,2S)-2-fluoro-1-cyclopropylamino]acetate
(1.08 g) was dissolved in 5 mL of ethanol, added with 10 mL of 3N
aqueous potassium hydroxide solution and heated to 50.degree. with
stirring. After 2 hours, the reaction mixture was ice-cooled,
acidified with 10% aqueous citric acid solution and extracted with
chloroform. An organic layer was dried with anhydrous sodium
sulfate. After filtration, the solvent was distilled away. The
resulting residue (0.89 g) was subjected to column chromatography
on silica gel to give the title compound as pale brown
crystals.
[0047] pale brown crystal; .sup.1H-NMR (270 MHz, CDCl.sub.3)
.delta.0.68-0.95 (m, 4H), 1.30-1.60 (m, 2H), 1.43 (s, 9H),
1.72-1.90 (m, 1H), 2.05-2.15 (m, 1H), 2.23-2.40 (m, 1H), 3.37-3.71
(m, 4H), 3.53 (s, 3H), 3.80-3.90 (m, 1H), 4.75-5.05 (md, J=51.9 Hz,
1H), 4.98 (brs, 1H), 6.94 (d, J=9.2 Hz, 1H), 8.07 (d, J=9.2 Hz,
1H), 8.66 (d, J=3.1 Hz, 1H), 15.20 (br s, 1H).
* * * * *