U.S. patent application number 12/567618 was filed with the patent office on 2010-02-11 for method and system of array imaging.
This patent application is currently assigned to Tufts University. Invention is credited to Sandra Bencic-Nagale, David R. Walt.
Application Number | 20100032547 12/567618 |
Document ID | / |
Family ID | 37766599 |
Filed Date | 2010-02-11 |
United States Patent
Application |
20100032547 |
Kind Code |
A1 |
Walt; David R. ; et
al. |
February 11, 2010 |
METHOD AND SYSTEM OF ARRAY IMAGING
Abstract
The present invention relates to a method and system of array
imaging that extends or maximizes the longevity of the sensor array
by minimizing the effects of photobleaching. The imaging system has
a light source, a variable exposure aperture, and a variable filter
system. The system extends the longevity of sensors by (1) using
the variable exposure aperture to selectively expose sections of
the sensor array containing representative numbers of each type of
sensor, and/or (2) using the variable filter system to control the
intensity of the excitation light, providing only the intensity
required to induce the appropriate excitation and increasing that
intensity over time as necessary to counteract the effects of
photobleaching.
Inventors: |
Walt; David R.; (Boston,
MA) ; Bencic-Nagale; Sandra; (Lowell, MA) |
Correspondence
Address: |
WOLF GREENFIELD & SACKS, P.C.
600 ATLANTIC AVENUE
BOSTON
MA
02210-2206
US
|
Assignee: |
Tufts University
Boston
MA
|
Family ID: |
37766599 |
Appl. No.: |
12/567618 |
Filed: |
September 25, 2009 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11209432 |
Aug 22, 2005 |
7595473 |
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12567618 |
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Current U.S.
Class: |
250/205 ;
250/226 |
Current CPC
Class: |
G01J 2001/0276 20130101;
G01J 3/2803 20130101; G01N 2021/6484 20130101; G01J 3/02 20130101;
G01N 2201/08 20130101; G01N 21/6428 20130101; G01N 21/6458
20130101; G01J 3/10 20130101; G01J 3/4406 20130101; G01N 2201/088
20130101; G01N 2021/6441 20130101; G01J 3/0213 20130101; G01J
3/0229 20130101 |
Class at
Publication: |
250/205 ;
250/226 |
International
Class: |
G01N 21/00 20060101
G01N021/00; H01L 31/0232 20060101 H01L031/0232 |
Goverment Interests
STATEMENT OF GOVERNMENT SPONSORED RESEARCH
[0001] The United States government may have certain rights in this
invention pursuant to Contract No. F49620-01-1-0395, awarded by the
United States Air Force, Office of Scientific Research.
Claims
1. An array imaging system comprising: (a) a light source; (b) an
array receiving component; (b) a variable exposure aperture
disposed between the light source and the array receiving
component; and (c) a variable filter system disposed between the
light source and the array receiving component.
2. The array imaging system of claim 1, further comprising a
magnification lens disposed between the light source and the array
receiving component.
3. The array imaging system of claim 2, further comprising an array
detector associated with the magnification lens.
4. The array imaging system of claim 1, wherein the variable filter
system is programmable to decrease in optical density over
time.
5. The array imaging system of claim 1, wherein the variable filter
system comprises a set of filters disposed between the light source
and the array receiving component, the set of filters being
programmed to provide decreasing optical density.
6. The array imaging system of claim 5, wherein the set of filters
comprises filters of varying optical densities.
7. The array imaging system of claim 5, wherein the set of filters
comprises filters having optical densities ranging from about 0.2
to about 1.0.
8. The array imaging system of claim 5, wherein the set of filters
provides optical density values ranging from about 1.0 to about
2.0.
9. The array imaging system of claim 1, wherein the variable
exposure aperture is a positionable aperture configured to operate
with the light source to control an area of exposure.
10. The array imaging system of claim 1, wherein the variable
exposure aperture comprises a slit configured to allow light to
pass through from the light source to the sensor array.
11. The array imaging system of claim 1, wherein the variable
exposure aperture comprises a rectangular slit.
12. A method of determining a property of a target analyte in a
sample comprising: contacting said sample with a sensor array
comprising a plurality of sensor element types and a plurality of
sensor elements of each sensor element type; illuminating a first
section of said sensor array with a first intensity and collecting
a first set of data; illuminating said first section of said sensor
array with a second intensity, wherein said second intensity
exceeds said first intensity by an amount that compensates for the
effect of photobleaching on said sensor elements, and collecting a
second set of data; and during at least one point during
performance of the method, determining at least one property of
said target analyte.
13. A method of imaging a sensor array that extends or maximizes
the longevity of the sensor array by minimizing the effects of
photobleaching, comprising: A) providing a sensor array comprising
a plurality of sensor elements of a plurality of sensor element
types; B) illuminating a first section of said sensor array with a
first intensity; C) illuminating said first section with a second
intensity, wherein said second intensity exceeds said first
intensity by an amount that compensates for the effects of
photobleaching on said sensor elements from said section; D)
illuminating a second section of said sensor array with a third
intensity, wherein said first section and said second section are
different; and E) illuminating said second section with a fourth
intensity, wherein said fourth intensity exceeds said third
intensity by an amount that compensates for the effects of
photobleaching on said sensor elements from said section.
14. The method of claim 13, wherein said sensor array comprises a
plurality of subsections collectively encompassing substantially
the entire array area, and wherein steps B-E are repeated until
each of said plurality of subsections has been illuminated at least
once.
15. The method of claim 13, wherein step C is repeated a plurality
of times, prior to performing step D.
16. The method of claim 13, wherein steps C and E are repeated a
plurality of times.
17. The method of claim 14, wherein steps C and E are repeated a
plurality of times until a plurality of said sensor elements of
each of said plurality of subsections of said array has been
substantially photobleached.
18. The method of claim 13, wherein the steps are completed in the
order recited.
19. The method of claim 13, wherein steps B and D are performed
prior to performing step C.
20. The method of claim 13, further comprising collecting a set of
data in at least one of steps B, C, D, or E.
21. The method of claim 13, further comprising the step of
determining at least one property of at least one analyte in a
sample contacted with said sensor array.
22. The method of claim 13, wherein said first section and said
second section are different.
23. The method of claim 13, wherein said first intensity and said
third intensity are approximately equal.
24. A method of imaging a sensor array that extends or maximizes
the longevity of the sensor array by minimizing the effects of
photobleaching, comprising: providing a sensor array comprising a
plurality of sensor elements of a plurality of sensor element
types; defining a plurality of subsections of the array, wherein
each subsection contains a distribution of each of the sensor
elements of each sensor element type that is substantially the same
as the overall distribution of each of the sensor elements of each
sensor element type for the entire sensor array; illuminating at
least a portion of the subsections of the array with a first
intensity; and illuminating a previously illuminated subsection of
the array with a second intensity, wherein said second intensity
exceeds said first intensity by an amount that compensates for the
effects of photobleaching on said sensor elements from said
section.
25. The method of claim 22, wherein in the first illuminating step,
each subsection is illuminated a plurality of times until a
plurality of said sensor elements of each subsection of said array
is substantially photobleached.
26. The method of claim 22, wherein the plurality of subsections
defined in the defining step collectively encompass essentially the
entirety of said sensor array and wherein each of the plurality of
subsections is illuminated at least once.
Description
CROSS-REFERENCE TO RELATED APPLICATION(S)
[0002] None.
FIELD OF THE INVENTION
[0003] The present invention relates to a method and system of
array imaging. More specifically, the present invention relates to
a method and system of array imaging that extends or maximizes the
longevity of the sensor array by minimizing the effects of
photobleaching.
BACKGROUND OF THE INVENTION
[0004] Fluorescence-based sensor longevity--the period during which
sensors exhibit reproducible responses when exposed to an
analyte--is limited. As the sensors are repeatedly exposed to light
over time, they become less responsive, typically due to
photobleaching. Certain sensor arrays retain responsiveness for an
average of approximately one hundred responses or a total exposure
time of less than 8 minutes.
[0005] The limited sensor life can be a limiting factor in the use
of sensor arrays. In some cases, replacement of non-responsive
sensors means having to replace the entire array or some portion
thereof.
[0006] Sensor arrays for analyzing vapors (also referred to as
"electronic noses"), for example, are limited by sensor life.
Electronic noses detect changes in the response patterns of
non-specific sensors exposed to vapors and categorize the vapors
with the use of pattern recognition software. Ideally, electronic
noses contain a diverse array of cross-reactive sensors. These
arrays are typically composed of conducting polymer sensors or
fluorescent sensors that produce patterns resulting from changes in
the properties of the sensors over time. Such arrays use pattern
recognition, storing the response patterns in a large database such
that a wide range of vapors can subsequently be identified using
the database. That is, during the training process, responses are
collected from known vapors and those responses are then used to
classify unknown vapors. A training database can be collected on
one array and applied to multiple arrays over time, which
eliminates the need for repetitive training. Ideally, a database
should contain many replicate array responses from many vapors. One
of the most important requirements for building large databases of
vapor patterns is array longevity. In fact, for many such
applications, only sensor arrays with long-term use capability and
with less frequent replacement and servicing needs will be
acceptable. It is understood that the more analytes that can be
recognized, the more effective the array, thereby underscoring the
need for an increased number of training replicates and inclusion
of additional vapors in the training database. If an array degrades
too fast, it will have to be replaced and the training will have to
be repeated. Thus, the longevity of most fluorescent sensor arrays
has typically been limited by the photobleaching rates of the
fluorescent dyes attached to the individual beads.
[0007] Several approaches to overcome or compensate for
photobleaching of fluorescent dyes and fluorescent sensors have
focused on the dyes or sensing substrates. For example, certain
ratiometric sensors have been introduced that are insensitive to
photobleaching effects. See Kermis, H. R.; Kostov, Y.; Harms, P.;
Rao, G. Biotechnol. Progress 2002, 18, 1047-1053; Song, A.; Parus,
S., Kopelman, R. Anal. Chem. 1997, 69, 863-867; and Xu, Z.;
Rollins, A.; Alcala, R.; Marchant, R. E. J. Biomed. Mater. Res.
1998, 39, 9-15. Further, anti-fading agents have been added to dyes
to chemically decrease the photobleaching rates of the dyes. See
Berrios, M.; Conlon, K. A.; Colflesh, D. E. Methods in Enzymology
1999, 307, 55-79; Krenik, K. D.; Kephart, G. M.; Offord, K. P.;
Dunnette, S. L.; Gleich, G. J. Journal of Immunological Methods
1989, 117, 91-97. Another approach involves increasing dye
photostability by engineering fluorescent polymer molecules with
structural properties that prevent .pi.-stacking, thereby
circumventing the fluorescence quenching caused by such
.pi.-stacking. See Yang, J.-S.; Swager, T. M. J. Am. Chem. Soc.
1998, 120, 5321-5322. Although somewhat successful, each of the
above methods requires chemical modification of the indicator dyes
or sensing substrates, or, in the case of ratiometric measurements,
the use of different indicators altogether.
[0008] Other approaches have taken advantage of the physical
properties of sensors to increase sensor longevity. One such
approach involves incorporating fluorescent dye into a polymer
matrix, resulting in a polymer layer containing freely diffusible
dye molecules. See Shortreed, M., Monson, E., and R. Kopelman, Anal
Chem. 1996, 68, 4015-4019; Barnard, S. M. and D. R. Walt, "Chemical
Sensors Based on Controlled-Release Polymer Systems," Science,
1991, 251 (4996): 927-9; Agayn, V. and D. R. Walt, "Fiber-Optic
Immunosensors Based on Continuous Reagent Delivery," Immunomethods,
1993, 3(2): 112-21; and Uttamial, M. and D. R. Walt, "A Fiber-Optic
Carbon Dioxide Sensor for Fermentation Monitoring," Bio/Technology,
1995, 13 (6): 597-601. In use, a small portion of the layer is
selectively illuminated and as the dye molecules are photobleached,
they are replaced with new dye molecules that diffuse from a
reservoir into the illuminated polymer film area, thereby
increasing the number of sensor measurements possible. Another
approach involving an IR detector focused on sectioning the array
with an optical slit. See Takayama, R.; Tomita, Y.; Asayama, J.;
Nomura, K., and H. Ogawa, Sens. Actuators A: Physical 1990, A22,
508-512. That is, the optical slit served to selectively illuminate
individual IR detectors in a microarray and the response of each
detector was monitored during and after the illumination.
[0009] However, the Shortreed et al and Walt et al methods
described above are limited to devices using the polymer layer
containing diffusible molecules. Thus, the methods cannot be
implemented using any known non-diffusible sensors. Further, the
Takayama et al. optical slit described above is used in conjunction
with an array comprised of only one type of sensor.
[0010] There is a need in the art for a method and system of array
imaging that extends or maximizes the longevity of the sensor array
by minimizing the effects of photobleaching.
BRIEF SUMMARY OF THE INVENTION
[0011] The present invention, in one embodiment, is a method of
detecting the presence of at least one target analyte in at least a
first and a second sample. The method includes contacting the first
sample with a sensor array comprising a plurality of sensor element
types and a plurality of sensor elements of each sensor element
type. The method further includes illuminating at least a first
section of the sensor array with a first intensity and collecting a
first set of data. In addition, the method includes contacting the
second sample with the sensor array and illuminating at least a
second section of the sensor array with a second intensity and
collecting a second set of data. Further, the method includes
determining the presence or absence of the target analytes. In one
aspect of this embodiment, the first and second sections are the
same, or alternatively they are different. The second intensity,
according to one embodiment, is greater than the first intensity,
or, alternatively, it is substantially similar to the first
intensity. Illuminating the sensor array can include illuminating
the sensor array through a variable exposure aperture, according to
one embodiment. The variable exposure aperture can be positionable.
It can also be a slit, including a rectangular-shaped slit.
[0012] The present invention, in another embodiment, is array
imaging system having a light source, an array receiving component,
a variable exposure aperture, and a variable filter system. The
variable exposure aperture is disposed between the light source and
the array receiving component, and the variable filter system is
disposed between the light source and the array receiving
component. The system can also, in one embodiment, have a
magnification lens disposed between the light source and the array
receiving component. In addition, the system can also have an array
detector associated with the magnification lens. The variable
filter system, according to one aspect of the invention, is
programmable to decrease in optical density over time. In one
embodiment, the variable filter system includes a set of filters
disposed between the light source and the array receiving
component, the set of filters being programmed to provide
decreasing optical density. The set of filters can have filters of
varying optical densities. In one aspect of the invention, the
variable exposure aperture is a positionable aperture configured to
operate with the light source to control an area of exposure. In a
further embodiment, the variable exposure aperture has a slit
configured to allow light to pass through from the light source to
the sensor array.
[0013] While multiple embodiments are disclosed, still other
embodiments of the present invention will become apparent to those
skilled in the art from the following detailed description, which
shows and describes illustrative embodiments of the invention. As
will be realized, the invention is capable of modifications in
various obvious aspects, all without departing from the spirit and
scope of the present invention. Accordingly, the drawings and
detailed description are to be regarded as illustrative in nature
and not restrictive.
BRIEF DESCRIPTION OF THE DRAWINGS
[0014] FIG. 1 is a schematic drawing showing an array imaging
system, according to one embodiment of the present invention.
[0015] FIG. 2 is a schematic drawing depicting selective
illumination of an array, according to one embodiment of the
present invention. As shown in the figure, moving a rectangular
field stop results in revealing a narrow section of the field of
view. Excitation light is masked through the rectangular field stop
to create a rectangle that is then reflected off the dichroic
mirror. The illuminated rectangle travels through the fiber to the
fluorescent bead sensors and passes back through a dichroic mirror
onto a CCD camera to create an image of the selectively illuminated
field of view. In this schematic representation, the components are
not drawn to scale and the mechanism of the rectangular motion is
simplified.
[0016] FIG. 3 is a CCD image of a field of view, according to one
embodiment of the present invention. The field of view was captured
at time=0 in (A), then subdivided into three sections where
segments are marked with different colors and clearly visible in
the insert (B). The sections were inspected starting from the first
illuminated rectangle at the top left and moving down to the right.
The small regions of photobleached areas at the end of the
longevity study are shown on the entire fiber, as well as in the
insert (C).
[0017] FIG. 4a depicts a graphical representation of a fluorescence
response corresponding to the vapor response of Alltech sensors
when illuminated using a system of the present invention according
to one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
[0018] FIG. 4b is a graphical representation depicting an SVD plot
corresponding to the vapor response of Alltech sensors when
illuminated using a system of the present invention according to
one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
[0019] FIG. 4c is a graphical representation showing a fluorescence
response corresponding to the vapor response of Chirex sensors when
illuminated using a system of the present invention according to
one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
[0020] FIG. 4d is a graphical representation depicting an SVD plot
corresponding to the vapor response of Chirex sensors when
illuminated using a system of the present invention according to
one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
[0021] FIG. 4e is a graphical representation showing a fluorescence
response corresponding to the vapor response of Snafl sensors when
illuminated using a system of the present invention according to
one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
[0022] FIG. 4f is a graphical representation depicting an SVD plot
corresponding to the vapor response of Snafl sensors when
illuminated using a system of the present invention according to
one embodiment. Each averaged sensor response was obtained by
averaging the normalized responses of the individual sensors
located on each subsection. The responses were processed using SVD
and their first three principal components were graphed. Legend:
DMMP--magenta, heptane--green, isopropenyl acetate--red,
p-cymene--purple, water--black.
DETAILED DESCRIPTION
[0023] The present invention relates to a method and system of
array imaging that extends or maximizes the longevity of the sensor
array by minimizing the effects of photobleaching. The imaging
system has a light source, a variable exposure aperture, and a
variable filter system. In use, the system extends the longevity of
sensors through an adaptive light exposure system by (1) using the
variable exposure aperture to selectively expose sections of the
sensor array containing representative numbers of each type of
sensor, and/or (2) using the variable filter system to control the
intensity of the excitation light, providing only the intensity
required to induce the appropriate excitation and increasing that
intensity over time as necessary to counteract the effects of
photobleaching. The reduction in light intensity and selective
illumination of sections of the array--rather than the entire
array--can result in increased sensor longevity and shorter
analysis time (because the limited exposure translates into fewer
sensors being used for data analysis).
[0024] FIG. 1 depicts an array imaging system 10 according to one
embodiment of the present invention. The system 10 has an
excitation light source 12, a variable exposure aperture 14, a
variable excitation filter system 16, and an array receiving
component 18, which is configured to receive the sensor array 20.
The exposure aperture 14 and the filter system 16 are positioned
between the light source 12 and the sensor array 20. The system
also includes a dichroic housing 22 containing a dichroic mirror
24, an emission filter system 26, an array detector 28, lens 30A,
lens 30B, and lens 30C. The dichroic housing 22, the dichroic
mirror 24, and the emission filter system 26 are positioned between
the sensor array 20 and the array detector 28. The lens 30A is
positioned between the sensor array 20 and the dichroic housing 22,
while the lenses 30B and 30C are positioned between the dichroic
mirror 24 and the array detector 28. More specifically, according
to one embodiment, lens 30B is positioned between the dichroic
housing 22 and the emission filter wheel 26, and lens 30C is
positioned between the emission filter wheel 26 and the array
detector 28. Alternatively, the lenses are positioned in any known
fashion.
[0025] In one aspect of the invention, the system 10 is any known
fluorescence microscope to which is added a variable exposure
aperture 14 and a variable excitation filter system 16. For
example, the fluorescence microscope can be the BX Olympus
horizontal microscope, available from Olympus. In a further
embodiment, the system 10 includes imaging software that can
control certain components of the system 10 automatically. For
example, the imaging software in one aspect of the invention can
control at least portions of the light source 12, the variable
exposure aperture 14 the filter systems 16, the emission filter
system 26, and the array detector 28. In one example, the imaging
software is the IPLab imaging software available from Scanalytics
in Fairfax, Va. Alternatively, the imaging software is any known
software for controlling components such as those listed above.
[0026] The excitation light source 12 can be any known light source
for use in a fluorescence microscope. In one example, the
excitation light source is a 75 Watt Xenon excitation source,
available from Ludl in Hawthorne, N.Y.
[0027] The variable exposure aperture 14 (also referred to herein
as a "slit") controls the size and shape of the field of excitation
light, thereby controlling the size and shape of the area of the
sensor array that is exposed by the light source 12. In one aspect
of the present invention, the slit 14 is an adjustable rectangular
field stop 14, which is a rectangular diaphragm having adjustable
portions that can be variably positioned to create a rectangular or
square excitation light field of any size to an accuracy of within
tenths of microns, limited only by the total size of the field. In
one alternative, the slit 14 is a diaphragm that can be variably
positioned to create a circular excitation light field of any size
to an accuracy of within tenths of microns. Thus, the adjustable
rectangular field stop 14 provides for illuminating any section of
the sensor array 20 with an illumination field of any predetermined
size and any shape that can be created by any known rectangular
field stop or diaphragm. According to one embodiment, the field
stop 14 forms an optical slit that creates an exposed area on the
array with dimensions of about 60 .mu.m by about 300 .mu.m.
Alternatively, the size of the slit can vary from about 100
.mu.m.times.100 .mu.m (which, in one embodiment, creates an exposed
area on the array of about 5 .mu.m.times.5 .mu.m at 20.times.
magnification) to about 7 mm.times.7 mm.
[0028] In one example, the variable exposure aperture 14 is a
rectangular field stop available from Olympus. Alternatively, the
variable exposure aperture 14 can be any known diaphragm, field
stop, or similar device for controlling the size and shape of the
field of excitation light, and thus the field can have any one of a
variety of geometries, such as square, rectangular, parallelogram,
and circular.
[0029] While the variable exposure aperture 14 as depicted in FIG.
1 is positioned between the excitation light source 12 and the
dichroic mirror 24, the aperture 14 can alternatively be positioned
in any focal point between the light source 12 and the sensor array
20.
[0030] As shown in FIG. 1, the variable excitation filter system 16
according to one embodiment has two components: (1) a single
neutral density ("ND") filter 16A, and (2) a variable filter
component 16B. In one aspect of the invention, the filter system 16
is an automated dual excitation filter wheel 16 that accommodates
both variable filters 16A and 16B. Such a wheel 16 allows for
providing any combination of the filters 16A and 16B. The single ND
filter 16A in one aspect of the invention has an optical density of
1.0. Alternatively, the ND filter 16A can have any known optical
density. In a further alternative, the system 16 has only a
variable filter component 16B.
[0031] The variable filter component 16B, according to one
embodiment, is a filter wheel 16B that provides 10 filters having
different optical densities, any of which can be used to provide
the desired reduction in illumination intensity. The wheel 16B can
also be set such that no filter is placed in the path of the
illumination. Alternatively, the filter wheel 16B can have any
known number of filters. According to one embodiment, the wheel 16B
has five filters with optical densities of 0.2, 0.4, 0.6, 0.8, and
1.0. Using the wheel 16B with those filters in combination with the
single ND filter 16A results in six different ND settings with the
following OD values: 2.0, 1.8, 1.6, 1.4, 1.2, and 1.0,
corresponding to 1%, 1.6%, 2.5%, 4%, 6.3%, and 10% transmittance,
respectively.
[0032] In one aspect of the present invention, the variable filter
system 16 provides filters providing a range of optical densities
from about 0 to about 4. Alternatively, the system 16 provides
filters providing a range of optical densities from about 1 to
about 2.
[0033] In one aspect of the invention, the filters of the variable
filter system 16 differ in optical density in increments of 0.2
(that is, each filter differs in optical density by 0.2 from each
of the filters that has a greater and a lesser optical density).
Alternatively, the variable filter system 16 has filters that
differ in optical density in increments of 0.1. In a further
alternative, the filters of the system 16 are provided in
increments of less than 0.1.
[0034] According to one embodiment, the filter wheel is an
automated filter wheel. One example of an automated filter wheel
that can be used in the present invention is the LEP filter wheel
with a MAC 5000 controller system, available from Ludl Electronic
Products Ltd. in Hawthorne, N.Y. In an alternative embodiment, the
variable excitation filter system 16 can include any known
component configured to allow for adjustable filter settings, such
as, for example, a filter slider or any similar device, thereby
providing for filters having any known optical density. In one
aspect of the invention, the filters positioned in the filter wheel
16B are absorptive neutral density ("ND") filters available from
Newport Optics in Irvine, Calif. Alternatively, the filters are any
known filters for use in a fluorescence microscope.
[0035] The array receiving component 18 as depicted in FIG. 1 is
positionable with respect to the rest of the system 10. In one
embodiment, the component 18 is positionable in the x-y direction.
Alternatively, the component 18 is positionable in the z direction
or in both the x-y direction and the z direction.
[0036] The sensor array 20 of the present invention is an array
having at least a first substrate with a surface comprising
individual sites. By "array" herein is meant a plurality of
candidate agents in an array format; the size of the array
depending on the composition and end use of the array. Arrays
containing from about 2 different active agents to many millions
can be made, with very large arrays, including very large fiber
optic arrays, being possible. Generally, the array will comprise
from two to as many as a billion or more, depending on the size of
the beads and the substrate, as well as the end use of the array,
thus very high density, high density, moderate density, low density
and very low density arrays may be made. Ranges for very high
density arrays, according to one embodiment, are from about
10,000,000 to about 2,000,000,000 (all numbers are per square cm),
while alternatively the ranges are from about 100,000,000 to about
1,000,000,000. High density arrays range about 100,000 to about
10,000,000, while alternatively, they range from about 1,000,000 to
about 5,000,000. Moderate density arrays range from about 10,000 to
about 100,000, and alternatively from about 20,000 to about 50,000.
Low density arrays are generally less than 10,000. In one
embodiment, the low density arrays range from about 1,000 to about
5,000. Very low density arrays are less than 1,000. According to
one embodiment, the very low density arrays range from about 10 to
about 1000, and alternatively range from about 100 to about 500. In
some embodiments, the compositions of the invention may not be in
array format; that is, for some embodiments, compositions
comprising a single active agent may be made as well. In addition,
in some arrays, multiple substrates may be used, either of
different or identical compositions. Thus for example, large arrays
may comprise a plurality of smaller substrates.
[0037] In addition, one advantage of the present compositions is
that particularly through the use of fiber optic technology,
extremely high density arrays can be made. Thus for example,
because beads of 200 .mu.m or less (with beads of 200 nm possible)
can be used, and very small fibers are known, it is possible to
have as many as 40,000 or more (in some instances, 1 million)
different fibers and beads in a 1 mm.sup.2 fiber optic bundle, with
densities of greater than 15,000,000 individual beads and fibers
(again, in some instances as many as 25-50 million) per 0.5
cm.sup.2 obtainable.
[0038] Suitable arrays include, but are not limited to, U.S. Pat.
Nos. 6,023,540, 6,266,459, 6,327,410, 6,429,027, 6,858,394, and
6,859,570, U.S. Published Applications 2005/0130188 and
2003/0027126, U.S. application Ser. No. 10/005,201, filed on Dec.
4, 2001, and Published PCT Application WO 00/60332, all of which
are incorporated herein by reference in their entirety.
[0039] By "nucleic acid" or "oligonucleotide" or grammatical
equivalents herein means at least two nucleotides covalently linked
together. A nucleic acid of the present invention will generally
contain phosphodiester bonds, although in some cases, as outlined
below, nucleic acid analogs are included that may have alternate
backbones, comprising, for example, phosphoramide (Beaucage, et
al., Tetrahedron, 49(10):1925 (1993) and references therein;
Letsinger, J. Org. Chem., 35:3800 (1970); Sprinzl, et al., Eur. J.
Biochem., 81:579 (1977); Letsinger, et al., Nucl. Acids Res.,
14:3487 (1986); Sawai, et al., Chem. Lett., 805 (1984), Letsinger,
et al., J. Am. Chem. Soc., 110:4470 (1988); and Pauwels, et al.,
Chemica Scripta, 26:141 (1986)), phosphorothioate (Mag, et al.,
Nucleic Acids Res., 19:1437 (1991); and U.S. Pat. No. 5,644,048),
phosphorodithioate (Briu, et al., J. Am. Chem. Soc., 111:2321
(1989)), O-methylphophoroamidite linkages (see Eckstein,
Oligonucleotides and Analogues: A Practical Approach, Oxford
University Press), and peptide nucleic acid backbones and linkages
(see Egholm, J. Am. Chem. Soc., 114:1895 (1992); Meier, et al.,
Chem. Int. Ed. Enql., 31:1008 (1992); Nielsen, Nature, 365:566
(1993); Carlsson, et al., Nature, 380:207 (1996), all of which are
incorporated by reference)). Other analog nucleic acids include
those with positive backbones (Denpcy, et al., Proc. Natl. Acad.
Sci. USA, 92:6097 (1995)); non-ionic backbones (U.S. Pat. Nos.
5,386,023; 5,637,684; 5,602,240; 5,216,141; and 4,469,863;
Kiedrowshi, et al., Angew. Chem. Intl. Ed. English, 30:423 (1991);
Letsinger, et al., J. Am. Chem. Soc., 110:4470 (1988); Letsinger,
et al., Nucleosides & Nucleotides, 13:1597 (1994); Chapters 2
and 3, ASC Symposium Series 580, "Carbohydrate Modifications in
Antisense Research", Ed. Y. S. Sanghui and P. Dan Cook; Mesmaeker,
et al., Bioorganic & Medicinal Chem. Lett., 4:395 (1994);
Jeffs, et al., J. Biomolecular NMR, 34:17 (1994); Tetrahedron
Lett., 37:743 (1996)) and non-ribose backbones, including those
described in U.S. Pat. Nos. 5,235,033 and 5,034,506, and Chapters 6
and 7, ASC Symposium Series 580, "Carbohydrate Modifications in
Antisense Research", Ed. Y. S. Sanghui and P. Dan Cook. Nucleic
acids containing one or more carbocyclic sugars are also included
within the definition of nucleic acids (see Jenkins, et al., Chem.
Soc. Rev., (1995) pp. 169-176). Several nucleic acid analogs are
described in Rawls, C & E News, June 2, 1997, page 35. All of
these references are hereby expressly incorporated by reference.
These modifications of the ribose-phosphate backbone may be done to
facilitate the addition of additional moieties such as labels, or
to increase the stability and half-life of such molecules in
physiological environments. According to one exemplary embodiment,
an analog nucleic acid useful in the present invention is PNA. In
addition, mixtures of naturally occurring nucleic acids and analogs
can be made. Alternatively, mixtures of different nucleic acid
analogs, and mixtures of naturally occurring nucleic acids and
analogs may be made. The nucleic acids may be single stranded or
double stranded, as specified, or contain portions of both double
stranded or single stranded sequence. The nucleic acid may be DNA,
both genomic and cDNA, RNA or a hybrid, where the nucleic acid
contains any combination of deoxyribo- and ribo-nucleotides, and
any combination of bases, including uracil, adenine, thymine,
cytosine, guanine, inosine, xanthanine, hypoxanthanine,
isocytosine, isoguanine, and base analogs such as nitropyrrole and
nitroindole, etc.
[0040] By "substrate" or "solid support" or other grammatical
equivalents herein is meant any material that can be modified to
contain discrete individual sites appropriate for the attachment or
association of beads and is amenable to at least one detection
method. As will be appreciated by those in the art, the number of
possible substrates is very large. Possible substrates include, but
are not limited to, glass and modified or functionalized glass,
plastics (including acrylics, polystyrene and copolymers of styrene
and other materials, polypropylene, polyethylene, polybutylene,
polyurethanes, Teflon, etc.), polysaccharides, nylon or
nitrocellulose, resins, silica or silica-based materials including
silicon and modified silicon, carbon, metals, inorganic glasses,
plastics, optical fiber bundles, and a variety of other polymers.
In general, the substrates allow optical detection and do not
themselves appreciably fluoresce.
[0041] Generally the substrate is flat (planar), although as will
be appreciated by those in the art, other configurations of
substrates may be used as well; for example, three dimensional
configurations can be used, for example by embedding the beads in a
porous block of plastic that allows sample access to the beads and
using a confocal microscope for detection. Similarly, the beads may
be placed on the inside surface of a tube, for flow-through sample
analysis to minimize sample volume. According to one embodiment,
substrates used with the present invention are optical fiber
bundles as discussed below, or, alternatively, flat planar
substrates such as glass, polystyrene and other plastics and
acrylics.
[0042] In one embodiment, the substrate is an optical fiber bundle
or array, as is generally described in U.S. Ser. Nos. 08/944,850
and 08/519,062, PCT US98/05025, and PCT US98/09163, all of which
are expressly incorporated herein by reference. Certain embodiments
utilize preformed unitary fiber optic arrays. By "preformed unitary
fiber optic array" herein is meant an array of discrete individual
fiber optic strands that are co-axially disposed and joined along
their lengths. The fiber strands are generally individually clad.
However, one thing that distinguished a preformed unitary array
from other fiber optic formats is that the fibers are not
individually physically manipulatable; that is, one strand
generally cannot be physically separated at any point along its
length from another fiber strand.
[0043] Generally, the array of array compositions of the invention
can be configured in several ways; see for example U.S. Ser. No.
09/473,904, hereby expressly incorporated by reference. In one
aspect of the invention, a first substrate comprising a plurality
of assay locations (sometimes also referred to herein as "assay
wells"), such as a microtiter plate, is configured such that each
assay location contains an individual array. That is, the assay
location and the array location are the same. For example, the
plastic material of the microtiter plate can be formed to contain a
plurality of "bead wells" in the bottom of each of the assay wells.
Beads containing the active agent of the invention can then be
loaded into the bead wells in each assay location as is more fully
described below.
[0044] In one embodiment in which the substrate has a plurality of
assay wells with a plurality of bead wells in the bottom of each
assay well, the bead wells are discrete individual sites
appropriate for the attachment or association of beads formed on
the inner surface of each assay well. Alternatively, the bead wells
are discrete individual sites formed on individual fibers of a
fiber optic bundle embedded in each assay well. For example, fiber
optic bundles can be embedded in the microtiter wells. In a further
alternative, the bead wells are formed on the flat surface of the
substrate, either for example by etching or drilling wells, or by
embedding fiber optic bundles in the slide (for example this can be
done by embedding fiber optic bundles in a block of glass or
plastic (or other materials) and then "slicing" planar sections. In
these embodiments, an additional substrate layer is placed on top
of the substrate (on the same side as the bead wells), wherein the
additional substrate layer has holes that correspond to the
locations of the bead wells. As such, the holes in the additional
substrate layer create assay wells in which bead wells formed in
the flat surface of the substrate are located within each of the
assay wells. For example, a silicon or rubber layer or gasket can
be applied, or a second sheet of plastic or glass. In a further
embodiment, the wells are contained on a flat surface and the assay
locations are created not by the use of a second physical layer,
but rather by the use of chemical functionality. For example, the
addition of hydrophobic solvent in a pattern allows for the
separation of hydrophilic samples; thus, for example, a "grid" of
hydrophobic material can be applied to fluidically separate the
array locations. Alternatively, other physical methodologies may be
used, such as etching.
[0045] In one aspect of the invention, the substrate is a
microtiter plate. Alternatively, the substrate is a microscope
slide. In a further alternative, the substrate is any known
substantially planar substrate.
[0046] At least one surface of the substrate is modified to contain
discrete, individual sites for later association of microspheres.
These sites may comprise physically altered sites, i.e. physical
configurations such as wells or small depressions in the substrate
that can retain the beads, such that a microsphere can rest in the
well, or the use of other forces (magnetic or compressive), or
chemically altered or active sites, such as chemically
functionalized sites, electrostatically altered sites,
hydrophobically/hydrophilically functionalized sites, spots of
adhesive, etc.
[0047] The sites may be a pattern, i.e. a regular design or
configuration, or randomly distributed. One embodiment utilizes a
regular pattern of sites such that the sites may be addressed in
the X-Y coordinate plane. "Pattern" in this sense includes a
repeating unit cell, preferably one that allows a high density of
beads on the substrate. However, it should be noted that these
sites may not be discrete sites. That is, it is possible to use a
uniform surface of adhesive or chemical functionalities, for
example, that allows the association of beads at any position. That
is, the surface of the substrate is modified to allow association
of the microspheres at individual sites, whether or not those sites
are contiguous or non-contiguous with other sites. Thus, the
surface of the substrate may be modified such that discrete sites
are formed that can only have a single associated bead, or
alternatively, the surface of the substrate is modified and beads
may go down anywhere, but they end up at discrete sites.
[0048] In one aspect of the present invention, the surface of the
substrate is modified to contain wells, i.e. depressions in the
surface of the substrate. This may be done as is generally known in
the art using a variety of techniques, including, but not limited
to, photolithography, stamping techniques, molding techniques and
microetching techniques. As will be appreciated by those in the
art, the technique used will depend on the composition and shape of
the substrate.
[0049] In one embodiment, physical alterations are made in a
surface of the substrate to produce the sites. According to one
aspect of the present invention, the substrate is a fiber optic
bundle and the surface of the substrate is a terminal end of the
fiber bundle, as is generally described in Ser. No. 08/818,199 and
09/151,877, both of which are hereby expressly incorporated by
reference. In this embodiment, wells are made in a terminal or
distal end of a fiber optic bundle comprising individual fibers. In
this embodiment, the cores of the individual fibers are etched,
with respect to the cladding, such that small wells or depressions
are formed at one end of the fibers. The required depth of the
wells will depend on the size of the beads to be added to the
wells.
[0050] Generally in this embodiment, the microspheres are
non-covalently associated in the wells, although the wells may
additionally be chemically functionalized as is generally described
below, cross-linking agents may be used, or a physical barrier may
be used, i.e. a film or membrane over the beads.
[0051] In accordance with one embodiment, the surface of the
substrate is modified to contain chemically modified sites, that
can be used to associate, either covalently or non-covalently, the
microspheres of the invention to the discrete sites or locations on
the substrate. "Chemically modified sites" in this context
includes, but is not limited to, the addition of a pattern of
chemical functional groups including amino groups, carboxy groups,
oxo groups and thiol groups, that can be used to covalently attach
microspheres, which generally also contain corresponding reactive
functional groups; the addition of a pattern of adhesive that can
be used to bind the microspheres (either by prior chemical
functionalization for the addition of the adhesive or direct
addition of the adhesive); the addition of a pattern of charged
groups (similar to the chemical functionalities) for the
electrostatic association of the microspheres, i.e. when the
microspheres comprise charged groups opposite to the sites; the
addition of a pattern of chemical functional groups that renders
the sites differentially hydrophobic or hydrophilic, such that the
addition of similarly hydrophobic or hydrophilic microspheres under
suitable experimental conditions will result in association of the
microspheres to the sites on the basis of hydroaffinity. For
example, the use of hydrophobic sites with hydrophobic beads, in an
aqueous system, drives the association of the beads preferentially
onto the sites. As outlined above, "pattern" in this sense includes
the use of a uniform treatment of the surface to allow association
of the beads at discrete sites, as well as treatment of the surface
resulting in discrete sites. As will be appreciated by those in the
art, this may be accomplished in a variety of ways.
[0052] By "microspheres" or "beads" or "particles" or grammatical
equivalents herein is meant small discrete particles. The
composition of the beads will vary, depending on the class of
active agent and the method of synthesis. Suitable bead
compositions include those used in peptide, nucleic acid and
organic moiety synthesis, including, but not limited to, plastics,
ceramics, glass, polystyrene, methylstyrene, acrylic polymers,
paramagnetic materials, thoria sol, carbon graphite, titanium
dioxide, latex or cross-linked dextrans such as Sepharose,
cellulose, nylon, cross-linked micelles and Teflon may all be used.
"Microsphere Detection Guide" from Bangs Laboratories, Fishers Ind.
is a helpful guide.
[0053] The beads need not be spherical; irregular particles may be
used. In addition, the beads may be porous, thus increasing the
surface area of the bead available for either active agent
attachment or IBL attachment. The bead sizes range from nanometers,
i.e. 100 nm, to millimeters, i.e. 1 mm, with beads from about 0.2
micron to about 200 microns, and alternatively from about 0.5 to
about 5 micron, although in some embodiments smaller beads may be
used.
[0054] It should be noted that a key component of the invention is
the use of a substrate/bead pairing that allows the association or
attachment of the beads at discrete sites on the surface of the
substrate, such that the beads do not move during the course of the
assay.
[0055] Each microsphere comprises an active agent, although as will
be appreciated by those in the art, there may be some microspheres
which do not contain an active agent, depending on the synthetic
methods. By "candidate active agent" or "active agent" or "chemical
functionality" or "binding ligand" as used herein describes any
molecule which can be attached to the microspheres of the invention
and is generally used for the detection of target analytes,
including both biological and chemical target analytes. According
to one embodiment, the active agent is a bioactive agent such as a
protein, oligopeptide, small organic molecule, coordination
complex, polysaccharide, polynucleotide, etc. Alternatively, the
active agent is a chemically active agent. It should be understood
that the compositions of the invention have two primary uses. In
one embodiment, as is more fully outlined below, the compositions
are used to detect the presence of a particular target analyte; for
example, the presence or absence of a particular nucleotide
sequence or a particular protein, such as an enzyme, an antibody or
an antigen. For example, the compositions can be used to detect
vapor phase analytes (e.g. volatile organic compounds, explosives,
chemical warfare agents, bacteria headspace, food aromas, etc.).
Alternatively, the compositions are used to screen active agents,
i.e. drug candidates, for binding to a particular target
analyte.
[0056] Bioactive agents encompass numerous chemical classes, though
typically they are organic molecules, preferably small organic
compounds having a molecular weight of more than 100 and less than
about 2,500 Daltons. Bioactive agents comprise functional groups
necessary for structural interaction with proteins, particularly
hydrogen bonding, and typically include at least an amine,
carbonyl, hydroxyl or carboxyl group, preferably at least two of
the functional chemical groups. The bioactive agents often comprise
cyclical carbon or heterocyclic structures and/or aromatic or
polyaromatic structures substituted with one or more of the above
functional groups. Bioactive agents are also found among
biomolecules including peptides, nucleic acids, saccharides, fatty
acids, steroids, purines, pyrimidines, derivatives, structural
analogs or combinations thereof. In one embodiment, the bioactive
agents are nucleic acids and proteins.
[0057] Active agents can be obtained from a wide variety of sources
including libraries of synthetic or natural compounds. For example,
numerous means are available for random and directed synthesis of a
wide variety of organic compounds and biomolecules, including
expression of randomized oligonucleotides. Alternatively, libraries
of natural compounds in the form of bacterial, fungal, plant and
animal extracts are available or readily produced. Additionally,
natural or synthetically produced libraries and compounds are
readily modified through conventional chemical, physical and
biochemical means. Known pharmacological agents may be subjected to
directed or random chemical modifications, such as acylation,
alkylation, esterification and/or amidification to produce
structural analogs.
[0058] In one aspect of the invention, the bioactive agents are
proteins. By "protein" herein is meant at least two covalently
attached amino acids, which includes proteins, polypeptides,
oligopeptides and peptides. The protein may be made up of naturally
occurring amino acids and peptide bonds, or synthetic
peptidomimetic structures. Thus "amino acid", or "peptide residue",
as used herein means both naturally occurring and synthetic amino
acids. For example, homo-phenylalanine, citrulline and norleucine
are considered amino acids for the purposes of the invention. The
side chains may be in either the (R) or the (S) configuration. In
this embodiment, the amino acids are in the (S) or L-configuration.
If non-naturally occurring side chains are used, non-amino acid
substituents may be used, for example to prevent or retard in vivo
degradations.
[0059] In one embodiment, the bioactive agents are naturally
occurring proteins or fragments of naturally occurring proteins.
Thus, for example, cellular extracts containing proteins, or random
or directed digests of proteinaceous cellular extracts, may be
used. In this way libraries of procaryotic and eukaryotic proteins
may be made for screening in the systems described herein. In one
embodiment, the bioactive agents are libraries of bacterial,
fungal, viral, and mammalian proteins, including, according to one
embodiment, human proteins.
[0060] The bioactive agents, in one aspect of the present
invention, are peptides of from about 5 to about 30 amino acids, or
alternatively are about 5 to about 20 amino acids, and in a further
alternative are about 7 to about 15 amino acids. The peptides may
be digests of naturally occurring proteins as is outlined above,
random peptides, or "biased" random peptides. By "randomized" or
grammatical equivalents herein is meant that each nucleic acid and
peptide consists of essentially random nucleotides and amino acids,
respectively. Since generally these random peptides (or nucleic
acids, discussed below) are chemically synthesized, they may
incorporate any nucleotide or amino acid at any position. The
synthetic process can be designed to generate randomized proteins
or nucleic acids, to allow the formation of all or most of the
possible combinations over the length of the sequence, thus forming
a library of randomized bioactive proteinaceous agents.
[0061] A library of active agents are used, according to one
embodiment. The library should provide a sufficiently structurally
diverse population of active agents to effect a probabilistically
sufficient range of binding to target analytes. Accordingly, an
interaction library must be large enough so that at least one of
its members will have a structure that gives it affinity for the
target analyte. Although it is difficult to gauge the required
absolute size of an interaction library, nature provides a hint
with the immune response: a diversity of 10.sup.7-10.sup.8
different antibodies provides at least one combination with
sufficient affinity to interact with most potential antigens faced
by an organism. Published in vitro selection techniques have also
shown that a library size of 10.sup.7 to 10.sup.8 is sufficient to
find structures with affinity for the target. Thus, in one aspect
of the invention, at least 10.sup.6, alternatively at least
10.sup.7, in a further alternative at least 10.sup.8, and in yet
another alternative at least 10.sup.9 different active agents are
simultaneously analyzed in the subject methods. According to one
embodiment, the methods maximize library size and diversity.
[0062] In one embodiment, the library is fully randomized, with no
sequence preferences or constants at any position. Alternatively,
the library is biased. That is, some positions within the sequence
are either held constant, or are selected from a limited number of
possibilities. For example, in one aspect of the invention, the
nucleotides or amino acid residues are randomized within a defined
class, for example, of hydrophobic amino acids, hydrophilic
residues, sterically biased (either small or large) residues,
towards the creation of cysteines, for cross-linking, prolines for
SH-3 domains, serines, threonines, tyrosines or histidines for
phosphorylation sites, etc., or to purines, etc.
[0063] In one aspect of the present invention, the bioactive agents
are nucleic acids (generally called "probe nucleic acids" or
"candidate probes" herein). By "nucleic acid" or "oligonucleotide"
or grammatical equivalents herein means at least two nucleotides
covalently linked together. A nucleic acid of the present invention
will generally contain phosphodiester bonds, although in some
cases, as outlined below, nucleic acid analogs are included that
may have alternate backbones, comprising, for example,
phosphoramide (Beaucage, et al., Tetrahedron, 49(10):1925 (1993)
and references therein; Letsinger, J. Org. Chem., 35:3800 (1970);
Sprinzl, et al., Eur. J. Biochem., 81:579 (1977); Letsinger, et
al., Nucl. Acids Res., 14:3487 (1986); Sawai, et al., Chem. Lett.,
805 (1984), Letsinger, et al., J. Am. Chem. Soc., 110:4470 (1988);
and Pauwels, et al., Chemica Scripta, 26:141 (1986)),
phosphorothioate (Mag, et al., Nucleic Acids Res., 19:1437 (1991);
and U.S. Pat. No. 5,644,048), phosphorodithioate (Briu, et al., J.
Am. Chem. Soc., 111:2321 (1989)), O-methylphophoroamidite linkages
(see Eckstein, Oligonucleotides and Analogues: A Practical
Approach, Oxford University Press), and peptide nucleic acid
backbones and linkages (see Egholm, J. Am. Chem. Soc., 114:1895
(1992); Meier, et al., Chem. Int. Ed. Engl., 31:1008 (1992);
Nielsen, Nature, 365:566 (1993); Carlsson, et al., Nature, 380:207
(1996), all of which are incorporated by reference)). Other analog
nucleic acids include those with positive backbones (Denpcy, et
al., Proc. Natl. Acad. Sci. USA, 92:6097 (1995)); non-ionic
backbones (U.S. Pat. Nos. 5,386,023; 5,637,684; 5,602,240;
5,216,141; and 4,469,863; Kiedrowshi, et al., Anqew. Chem. Intl.
Ed. English, 30:423 (1991); Letsinger, et al., J. Am. Chem. Soc.,
110:4470 (1988); Letsinger, et al., Nucleosides & Nucleotides,
13:1597 (1994); Chapters 2 and 3, ASC Symposium Series 580,
"Carbohydrate Modifications in Antisense Research", Ed. Y. S.
Sanghui and P. Dan Cook; Mesmaeker, et al., Bioorqanic &
Medicinal Chem. Lett., 4:395 (1994); Jeffs, et al., J. Biomolecular
NMR, 34:17 (1994); Tetrahedron Lett., 37:743 (1996)) and non-ribose
backbones, including those described in U.S. Pat. Nos. 5,235,033
and 5,034,506, and Chapters 6 and 7, ASC Symposium Series 580,
"Carbohydrate Modifications in Antisense Research", Ed. Y. S.
Sanghui and P. Dan Cook. Nucleic acids containing one or more
carbocyclic sugars are also included within the definition of
nucleic acids (see Jenkins, et al., Chem. Soc. Rev., (1995) pp.
169-176). Several nucleic acid analogs are described in Rawls, C
& E News, Jun. 2, 1997, page 35. All of these references are
hereby expressly incorporated by reference. These modifications of
the ribose-phosphate backbone may be done to facilitate the
addition of additional moieties such as labels, or to increase the
stability and half-life of such molecules in physiological
environments. For example, PNA is a nucleic acid analog in one
embodiment of the present invention. In addition, mixtures of
naturally occurring nucleic acids and analogs can be made.
Alternatively, mixtures of different nucleic acid analogs, and
mixtures of naturally occurring nucleic acids and analogs may be
made. The nucleic acids may be single stranded or double stranded,
as specified, or contain portions of both double stranded or single
stranded sequence. The nucleic acid may be DNA, both genomic and
cDNA, RNA or a hybrid, where the nucleic acid contains any
combination of deoxyribo- and ribo-nucleotides, and any combination
of bases, including uracil, adenine, thymine, cytosine, guanine,
inosine, xanthanine, hypoxanthanine, isocytosine, isoguanine, and
base analogs such as nitropyrrole and nitroindole, etc.
[0064] In one embodiment, the bioactive agents are libraries of
clonal nucleic acids, including DNA and RNA. In this embodiment,
individual nucleic acids are prepared, generally using conventional
methods (including, but not limited to, propagation in plasmid or
phage vectors, amplification techniques including PCR, etc.). The
nucleic acids are preferably arrayed in some format, such as a
microtiter plate format, and beads added for attachment of the
libraries.
[0065] Attachment of the clonal libraries (or any of the nucleic
acids outlined herein) may be done in a variety of ways, as will be
appreciated by those in the art, including, but not limited to,
chemical or affinity capture (for example, including the
incorporation of derivatized nucleotides such as AminoLink or
biotinylated nucleotides that can then be used to attach the
nucleic acid to a surface, as well as affinity capture by
hybridization), cross-linking, and electrostatic attachment,
etc.
[0066] By "labeled" herein is meant that a compound has at least
one element, isotope or chemical compound attached to enable the
detection of the compound. In general, labels fall into three
classes: a) isotopic labels, which may be radioactive or heavy
isotopes; b) magnetic, electrical, thermal; and c) colored or
luminescent dyes; although labels include enzymes and particles
such as magnetic particles as well. According to one embodiment,
labels used with the present invention include luminescent labels.
Suitable labels include, but are not limited to, fluorescent
lanthanide complexes, including those of Europium and Terbium,
fluorescein, rhodamine, tetramethylrhodamine, eosin, erythrosin,
coumarin, methyl-coumarins, pyrene, Malacite green, stilbene,
Lucifer Yellow, Cascade Blue.TM., Texas Red, FITC, PE, cy3, cy5 and
others described in the 6th Edition of the Molecular Probes
Handbook by Richard P. Haugland, hereby expressly incorporated by
reference.
[0067] The sensor array 20, in accordance with one aspect of the
present invention, is a microbead sensor array 20. In one
embodiment, the use of a substrate/bead pairing allows the
association or attachment of the beads at discrete sites on the
surface of the substrate, such that the beads do not move during
the course of the assay.
[0068] In certain embodiments in which the array 20 is a bead
sensor array 20, the present invention includes a population of
microspheres. By "population" herein is meant a plurality of
sensors as outlined above for arrays. Where the array 20 is a bead
sensor array, the population is a plurality of microspheres.
[0069] Within the population are separate subpopulations (also
referred to herein as "types," "groups," or "subsets"), which can
be a single sensor or multiple identical sensors. That is, in some
embodiments, as is more fully outlined below, the array may contain
only a single sensor for each active agent; while other embodiments
utilize a plurality of sensors of each type.
[0070] In embodiments in which the sensor array is a microsphere
sensor array, the subpopulations which are characterized by the
active agent attached to the microsphere and can be a single
microsphere or multiple identical microspheres. According to one
aspect of the invention, the use of more than one subpopulation,
wherein each subpopulation comprises a plurality of microspheres
containing the same active agent, provides a redundancy that is
built into the system. That is, providing subpopulations or
subgroups of microspheres having multiple microspheres containing
the same active agent ensures that numerous microsphere replicates
exist within any array of the present invention.
[0071] The array 20 according to one embodiment has a population of
24,000 microbead sensors randomly distributed across the array in
wells etched on one end of optical imaging fibers. Each sensor
falls into one of four sensor subpopulations or types (also
referred to as "groups" or "subsets") such that there are
statistically approximately 6,000 replicates of each sensor type.
In the present invention, a substantial number of replicates
ensures that exposure of selected portions of an array results in
exposure of a statistically significant number of each type of
sensor. Alternatively, the array 20 has from about 20 to about
50,000 microbead sensors and from about 4 to about 15 sensor types.
In a further alternative, the array 20 has any known number of
microbead sensors and any known number of sensor types for
identifying target analytes in an array imaging system. The
microbead sensors that can be used in the present invention
include, but are not limited to, Alltech, Chirex, Sel, or Snafl
bead sensors.
[0072] As is understood in the art, the microbead/optical imaging
fiber array 20 is typically positioned such that the excitation
light from the light source 12 strikes the proximal faces of the
fibers in the array 20 and passes through the fibers to the
microbead sensors positioned on the distal face of the fibers.
According to certain embodiments of the present invention, the
variable exposure aperture 14 creates an excitation light field
that strikes only a small portion of the proximal fiber faces such
that when the light passes through those particular fibers, only a
corresponding small portion of the microbead sensors positioned on
the distal face of those fibers are struck by the excitation light.
As a result, only a section of the microbead sensors in the sensor
array 20 are illuminated. Alternatively, the fiber array 20 can be
used simply as a substrate for holding beads and is not used to
convey excitation light to the distal face.
[0073] The sensor array 20, according to an alternative embodiment,
has any known type of sensors, including, but not limited to, any
known non-bead sensors. In another alternative, the substrate does
not comprise an optical fiber bundle or optical fiber array.
[0074] The dichroic housing 22 illustrated in FIG. 1 according to
one embodiment of the invention contains a dichroic mirror 24,
which can be any known dichroic mirror for use in a fluorescence
microscope. The housing 22, in one alternative aspect, can contain
an additional excitation filter and/or emission filter, often
configured as a filter "cube," which is known in the art.
[0075] In one aspect of the invention, the lens 30A is either a
10.times. (0-30 NA) objective lens or a 20.times. (0.50 NA)
objective lens. Alternatively, the lens 30A can have any known
magnification. The lenses 30B and 30C, according to one embodiment,
are a 1.6.times. magnification changer projection lens 30B and a
0.5.times. camera adapter projection lens 30C, both of which, in
one example, can be purchased from Olympus. Alternatively, the
lenses 30B, 30C can have any known magnification. Each of the
lenses 30A, 30B, 30C can be any known lens for use in a
fluorescence microscope.
[0076] The emission filter system 26 can be any known emission
filter system for use in a fluorescence microscope. In one example,
the emission filter system is an automated filter wheel.
[0077] The array detector 28, according to one embodiment, is a CCD
camera. Alternatively, the array detector 28 can be any known array
detector, including any known camera, for use in a fluorescence
microscope. In one example, the array detector is a 1376.times.1040
pixel Sensicam QE CCD Camera, available from Cooke Corp. in
Romulus, Mich. In one aspect of the invention, pixel binning can be
employed to enhance the sensitivity of the CCD camera. Pixel
binning is a known method for improving the camera's
signal-to-noise ratio, increasing the frame rate, and compressing
data collected by the camera by combining the pixel charges of
square groups of pixels into one "super pixel." In one embodiment,
4.times.4 binning was employed in the present invention. 4.times.4
binning involves summing the charge values of 16 adjacent
pixels.
[0078] According to one embodiment as shown in FIG. 1, the array
imaging system 10 is used to analyze vapors. In such embodiments,
the system 10 has a vapor delivery component 32 (also referred to
as a "sparge line") attached to a positioning component 34. The
vapor delivery component 32 receives the vapor to be tested from a
vapor delivery system 36 and delivers it to the sensor array 20.
According to one embodiment, the vapor delivery system 36 has
airtight bubblers placed in a temperature-controlled oven set at
25.degree. C. to maintain constant vapor pressures and
concentrations of analytes. Alternatively, the vapor delivery
system 36 can be any known device for delivering vapor samples to a
microscope. In one example, the vapor delivery system 36 is an
eight-channel automated vapor delivery system similar to the GDS
6000 Gas Delivery System, which is a six-channel system available
from Sensor Research and Development in Orono, Me.
(www.srdcorp.com). Alternatively, the array imaging system 10 is
used to analyze liquids or any substance known to be tested by
array imaging systems.
[0079] In one aspect of the present invention, the vapor delivery
system 36 is configured to deliver either pulses of vapor to be
tested or ultra-pure nitrogen. The system 36 in one embodiment has
a trigger mechanism (not shown) to allow the system 36 to alternate
between delivery of vapor pulses and nitrogen. In one example, the
trigger mechanism is a solenoid trigger. The system 36 delivers the
nitrogen at a constant 200 mL/min flow rate. Alternatively, any
effective flow rate can be used.
[0080] The vapor delivery system 36 can be, in one aspect of the
invention, a programmed, automated delivery system 36. The
automated vapor delivery system, in one embodiment, has vapor
delivery software that controls the system 36. In one example, the
vapor delivery software is known vapor delivery software with a
custom-developed software user interface by Sensor Research and
Development. Alternatively, the vapor delivery software can be any
known software capable of controlling the delivery of vapor to a
sensor array.
[0081] In use, the present invention according to one embodiment is
a method of detecting the presence of at least one target analyte
in a sample. The method includes contacting a sample with a sensor
array, illuminating a first section of the sensor array with a
first intensity and collecting data. In one embodiment, the method
also includes contacting a second sample with the sensor array,
illuminating a second section of the sensor array with a second
intensity and collecting data, and determining the presence or
absence of the target analyte.
[0082] In one aspect of the invention, the first section and second
section can be the same section or different sections. That is, the
method can include contacting a first sample with an array,
illuminating a section (also referred to herein as a "portion" or
"subsection") of the array, collecting data relating to the first
sample, then contacting a second sample with the array,
illuminating the same section of the array, and collecting data
relating to the second sample. Alternatively, the method can
include contacting a first sample with an array, illuminating a
first section of the array, collecting data relating to the first
sample, contacting a second sample with the array, illuminating a
second, different section of the array, and collecting data
relating to the second sample
[0083] In one embodiment of the invention, the first intensity and
second intensity can be the same or different. That is, the method
can include contacting a first sample with an array, illuminating a
section with a first intensity, collecting data relating to the
first sample, then contacting a second sample with the array,
illuminating the same section or a different section with a second
intensity that is the same as the first intensity, and collecting
data relating to the second sample. Alternatively, the method
includes contacting a first sample with an array, illuminating a
section with a first intensity, collecting data relating to the
first sample, then contacting a second sample with the array,
illuminating the same section or a different section with a second
intensity that is different than the first intensity, and
collecting data relating to the second sample. According to one
embodiment, the second intensity is greater than the first
intensity. In one aspect of the invention, the second intensity is
greater than the first intensity by an amount that compensates for
the effect of photobleaching on the sensors being illuminated. The
first intensity, according to one embodiment, is limited to the
minimum intensity sufficient to excite the sensors and create an
observable response.
[0084] According to one embodiment, the step of contacting the
sample with the sensor array includes adding the sample to the
array under conditions suitable for binding of the target analyte
to at least one of the active agents, i.e., generally physiological
conditions. Alternatively, the sensor array can be caused to come
into contact with the sample by any known method. According to one
embodiment in which the sample is a vapor sample, the vapor
delivery component 32 receives the vapor to be tested from a vapor
delivery system 36 and delivers it to the sensor array 20. The
vapor can comprise 50% saturated in air and saturated water vapor.
Alternatively, the vapor can comprise any known mixture of the
target analyte and water vapor. In one embodiment, the vapor is
delivered to the sensor array at 200 mL/min for a 1.6 second vapor
pulse. Alternatively, the vapor is delivered to the sensor array at
an amount and rate that ranges from about 10 mL/min to about 1900
mL/min and a vapor pulse time that ranges from about 0.02 seconds
to about 100 seconds. In a further alternative, the vapor is
delivered at an amount and rate that ranges from about 10 mL/min to
about 20 mL/min and a vapor pulse time of from about 0.2 seconds to
about 3.0 seconds. In yet another alternative, the vapor is
delivered at any known amount and rate for any known pulse
time.
[0085] According to one embodiment, each vapor pulse is followed by
a purge, in which air that does not contain the target analyte is
pumped through the system of the present invention. In one aspect
of the invention, the purge lasts for 60 seconds at a flow rate of
200 mL/min, with the air being directed to waste for the first 45
seconds and being directed to the sensors for the following 15
seconds. Alternatively, the purge can last for any known length of
time and can be performed in any known fashion.
[0086] In one aspect of the present invention, the array is
"decoded" in a process that is also referred to as "array
registration" prior contacting the array with a target analyte.
Because the array according to certain embodiments contains
randomly distributed sensors, the array registration or decoding
process identifies the type of sensor at each location in the
array. According to one embodiment, the registration process
involves contacting the array with a test composition for which
each of the sensor subtypes have a known response. That is, the
array is contacted with the test composition and the response is
recorded or "registered." Based on the response and a comparison
with the expected responses of each of the sensor subpopulations,
each bead in each location on the array is identified as belonging
to a particular subpopulation. According to one embodiment, the
test composition is dimethyl methylphosphonate ("DMMP").
Alternatively, the test composition is any known substance for
which the resulting response of each bead subpopulation is
known.
[0087] The step of illuminating a section of the sensor array,
according to one embodiment, involves activating an excitation
light source (such as the light source 12 in FIG. 1) such that
light is emitted by the light source. In one aspect of the
invention, the light strikes the desired sensors of the array such
that the sensors are excited and emit a fluorescent light in
response to the excitation light. According to one embodiment of
the invention, if a target analyte has bound to the active agent on
any given sensor which is struck by the excitation light, the color
and/or intensity of the fluorescent light emitted by the sensor
differs from the color and/or intensity that would be emitted in
the absence of the target analyte. The illumination (also referred
to herein as "excitation illumination" or "exposure" or "excitation
exposure") can last for 2.5 seconds. Alternatively, the exposure
period can range from about 0.02 seconds to about 100 seconds. In a
further alternative, the exposure period can be of any known
length.
[0088] According to one embodiment, the method further includes
controlling the field of light such that light strikes only a
desired subsection of the array. According to one embodiment, the
control of the light field is accomplished by passing the light
through a variable exposure aperture (such as the variable exposure
aperture 14 in FIG. 1), which operates to restrict the field of
light to a desired size and location such that when it strikes the
array, it strikes only the desired subsection. Alternatively, the
field of light can be controlled by any known method or apparatus
such that it strikes only the desired subsection of the array. This
restriction of the excitation light field to strike only a
subsection of the array sensors can provide statistically
significant results because of the distribution of the sensor
subpopulations across the array. That is, because there are many
replicates of each sensor type or subpopulation in every subsection
of the array, there are always a representative number of sensors
of each type present in the subsection to provide meaningful
response data. In one example, the array comprises a population of
24,000 total microbead sensors, each sensor being characterized as
one of four subpopulations. Thus, there are around 6,000 sensors in
each subpopulation, with the sensors distributed across the array
in such a fashion that any subsection of the array has a
representative sample of each subpopulation. Alternatively, the
array can have any number of sensors and any number of
subpopulations so long as the number of beads from each
subpopulation in any subsection to be illuminated consists of a
representative number of beads from each subpopulation.
[0089] In addition, the method according to one embodiment can also
include collecting data relating to the excitation of sensors
caused by the illumination. In one aspect of the invention, the
data collection involves recording fluorescence color and intensity
caused by excitation of the desired sensors. The data collection
can include recording the sensor response with an array detector
that is a camera (such as, for example, the camera 28 in FIG.
1).
[0090] According to one embodiment, the steps of contacting the
sample with the sensor array, illuminating the array, and
collecting data are entirely automated. For example, the automation
of the steps can be accomplished by integrating the imaging
software in the system 10 with the vapor delivery software in the
vapor delivery system 36.
[0091] The step of contacting the sample with the sensor array in
an automated fashion, according to one embodiment, includes the
sensor array imaging software triggering the vapor delivery
software to deliver the desired composition and flow of the desired
vapor mixture to the component 32 and then to the array 20. In one
example, the main imaging software script, which contains all the
commands necessary to collect response data, triggers a vapor
delivery software command line. In one embodiment, each vapor
delivery software command line contains information about
composition and flow of the desired vapor mixture and all such
command lines are programmed and stored in a command list. As
triggered by the appropriate command line in the vapor delivery
software, the vapor is directed to component 32 and delivered to
the sensor array 20. According to one embodiment, there is a time
delay prior to directing the vapor to component 32 in order to
purge the vapor lines of the system 36 with the new vapor.
[0092] The steps of illuminating the array and collecting data in
an automated fashion are controlled, in one aspect of the
invention, by the imaging software. During delivery of the vapor to
the sensor array 20, the imaging software controls certain
components of the system 10 such as, for example, the shutters of
the filters 16A, 16B, 26, the camera 28, and the trigger mechanism
of the vapor delivery system 36, such that the response data is
collected by the camera 28 and automatically saved to a CPU or
similar device.
[0093] According to one embodiment, the steps of automatically
contacting the sample with the sensor array, illuminating the
array, and collecting data are repeated in cycles of contacting,
illuminating, and collecting until all desired response data is
collected. For example, after the first cycle of contacting,
illuminating, and collecting is completed and the response is
saved, the imaging software starts the next cycle by triggering the
vapor delivery software to generate the next vapor. According to
one embodiment, this process continues until the list with a
predetermined number of command lines in the vapor delivery
software ends and the respective vapor responses have been saved.
This integration of the imaging and vapor delivery software enables
the control of the preparation, delivery, and response acquisition
of a list that contains an infinite number of vapor mixtures.
[0094] According to one embodiment of the present invention, the
process of contacting a second sample with the sensor array is
similar to contacting the first sample with the array. As described
above, the sample can be contacted with the sensor array by any
known method.
[0095] Illuminating a second section of the sensor array, according
to one embodiment of the present invention, involves activating the
excitation light source (such as the light source 12 in FIG. 1)
such that light is emitted by the light source, as described above.
The illuminating step can also include controlling or
"repositioning" the field of excitation light such that it strikes
a desired section of the array that is different from the first
section that was exposed. The repositioning according to one aspect
of the invention can be accomplished by passing the light through
the variable exposure aperture (such as the variable exposure
aperture 14 in FIG. 1), which operates to restrict the field of
light to a desired size and location. Typically, the repositioning
moves the field of excitation light to a predetermined position
such that the light does not strike any portion of the array
illuminated in the previous illumination step. In one aspect of the
invention, the subsections of the array are predetermined such that
each subsection constitutes a distinct portion of the array and at
least two repositioning steps occur in a successive fashion such
that each illumination strikes another subsection until each
subsection has been illuminated prior to repeating the cycle of
illumination. Alternatively, the field of light need not be
repositioned. That is, the illuminated section of the array remains
the same section illuminated previously.
[0096] Illuminating a second section of the sensor array, according
to one embodiment of the present invention, can also involve
illuminating the section with excitation light of a second
intensity. According to one embodiment, the second intensity is
greater than the first intensity. In one particular aspect of the
invention, the first intensity is of a predetermined intensity that
is a minimum intensity required to provide an observable,
collectable response from the sensors and the second intensity is
of an intensity greater than the first intensity only by that
amount that is required to compensate for any decrease in the
responsiveness of the sensors as a result of photobleaching (or for
any other reason). In one alternative of the invention, the second
intensity is predetermined by software.
[0097] The collection of a second set of data relating to the
illumination of the second section is similar to the collection of
the first set of data as described above.
[0098] In one aspect of the invention, the data are subsequently
analyzed. The data can be analyzed using data processing
software.
[0099] In one example, the data are pre-processed prior to analysis
using custom-designed data processing software to normalize and
average the responses of the selected sensors to each analyte by
sensor type. Normalization involves taking the intensities of each
vapor response, subtracting the initial background level from all
the intensity values and dividing the intensities by the highest
signal for that particular response. In this way, all vapor
responses have normalized intensities between one and minus one.
The responses of all the identical sensors in a particular
subsection, according to one embodiment, are combined and averaged
to provide an averaged response for a vapor pulse. Each vapor
exposure in this embodiment consists of four averaged responses for
the four sensor types represented per array subsection.
[0100] The data analysis, according to one embodiment, is performed
using known methods of pattern recognition, including pattern
recognition algorithms.
[0101] According to one embodiment, the method of the present
invention can be repeated multiple times. The attenuation of the
excitation light combined with the selective exposure of
subsections of the array can minimize the photobleaching rates of
the sensors such that the sensors last longer than under ordinary
use. In addition to the increased longevity, an additional benefit
to this approach is the shorter analysis time, since fewer sensors
are used for data analysis.
Example
[0102] The following examples are presented by way of
demonstration, and not limitation, of the invention. Unless
indicated otherwise, the following testing procedures and equipment
were employed.
[0103] Methods and Materials
[0104] Sensor and Array Fabrication. The Alitech, Chirex, Sel, and
Snafl bead sensors were similar to those reported previously (See
Stitzel, S. E.; Cowen, L. J.; Albert, K. J.; Walt, D. R. Anal.
Chem., 2001; Vol. 73, pp 5266-5271 ("Stitzel")).
[0105] With respect to materials, Nile Red dye was purchased from
Aldrich (Milwaukee, Wis.). SNAFL dye with a succinimidyl ester
functional group was purchased from Molecular Probes (Eugene,
Oreg.). Platinum EPS C4 and IBSil (NH.sub.2) beads were purchased
as dry stocks from Alltech (Deerfield, Ill.) and Phenomenex
(Torrance, Calif.), respectively. Chirex 3012 and Selectosil (SCX)
beads were retrieved from Phenomenex liquid chromatography columns,
Polished and etched optical fiber bundles with approximately 24,000
4.5 .mu.m-diameter wells were obtained from Illumina (San Diego,
Calif.).
[0106] With respect to sensor fabrication, Silica beads were
removed from chromatography columns, washed with toluene, and
allowed to dry overnight at 60.degree. C. The preparation of
dye-coated microbead sensors required either adsorption of Nile Red
or covalent attachment of SNAFL dye molecules onto the surface of
the beads. Dye solutions were combined with bead stocks (0.1 mL dye
solution per mg of beads) in 4-mL glass vials and mixed for 1 hour.
Table 1 lists the bead material specifications and the types and
concentrations of dyes used. After mixing, bead suspensions were
filtered and rinsed with either DMF or toluene. The Alitech,
Chirex, and Snafl sensor stocks were dried at 60.degree. C. for 16
hours, and Sel beads were dried for 3 hours. Dry sensor stocks were
stored in a dessicator until use. A mixture of equal weights of
these sensor stocks was then prepared for the fabrication of
randomized bead sensor arrays. Arrays with high sensor packing
density and uniform distribution across the etched face of the
fiber were prepared by gently tapping the etched fiber ends with
the dry bead mixture. Excess beads were then wiped off the surface
of the fiber bundle with a dust-free Texwipe clean room swab
(Fisher Scientific).
[0107] After acquisition of responses used in array registration,
the sensors were preconditioned with a 10-min saturated water vapor
purge, followed by a 50-min air purge. Water vapor and air were
delivered to the sensors at 200 mL/min. The preconditioning
protocol was developed because irreproducibility of Snafl sensor
responses was observed in the beginning of array usage when new
arrays, prepared from the dry sensor stock, were positioned on the
imaging setup. Our control experiments indicated that Snafl sensors
responded reproducibly after they were humidified and then
immediately dried in air. The emission bands of the SNAFL-coated
microbead sensors shifted and their intensities changed after the
preconditioning step, indicating that the surface pH might have
affected the dye's fluorescence properties. The first set of vapor
responses acquired with sensors on the first sub-section at 2.0 ND
was also discarded because some of the Snafl responses to vapors
were still inconsistent. The Snafl responses acquired during the
remainder of the longevity experiment were reproducible and were
therefore included in the data analysis.
TABLE-US-00001 TABLE 1 Sensor materials and fabrication conditions
Sensor Name Bead Type, Diameter Surface Functionality Dye (Conc.)
Alltech Alltech EPS C4, 5 mm --C.sub.4H.sub.10 Nile Red (0.5 mg/mL
toluene) Chirex Chirex 3012, 5 mm (R)-phenylglycine and Nile Red
(0.5 mg/mL toluene) 3,5-dinitroaniline urea linkage Sel Selectosil
(SCX), 5 mm cation exchange Nile Red (0.01 mg/mL toluene) Snafl
IBSil (NH.sub.2), 3 mm --NH.sub.2 SNAFL (0.2 mg/mL DMF)
[0108] Imaging System. The imaging system consisted of a BX Olympus
horizontal microscope, equipped with automated excitation and
emission filter wheels, a 75W Xenon excitation source (Ludl,
Hawthorne, N.Y.), and a 1376.times.1040 pixel Sensicam QE CCD
Camera (Cooke Corp., Auburn Hills, Mich.). 4.times.4 binning was
employed. Either a 10.times. (0.30 NA) or a 20.times. objective
(0.50 NA) was used for magnification in combination with 1.6.times.
and 0.5.times. lenses positioned between the microbead array and
the CCD camera. The variable exposure aperture used with the
microscope was a rectangular field stop (Olympus), a rectangular
version of a diaphragm that contracted and expanded the field of
view in the shape of a rectangle. The variable filter system used
with the microscope comprised absorptive neutral density (ND)
filters (Newport Optics, Irvine, Calif.) positioned in a filter
wheel. Combinations of filters with 1.0, 0.8, 0.6, 0.4, and 0.2
optical densities (OD) were changed sequentially in order of
decreasing OD. Six different ND settings with the following OD
values were used: 2.0, 1.8, 1.6, 1.4, 1.2, and 1.0, corresponding
to 1%, 1.6%, 2.5%, 4%, 6.3%, and 10% transmittance,
respectively.
[0109] Selective Exposure of Array Subsections. The rectangular
boundaries of a subsection of the array were confined and moved
across the entire field of view with adjustment screws that
controlled horizontal and vertical movement. In brief, the first
rectangular subsection on the top left side of the field of view
was selected, as shown in FIG. 2. When data collection on the first
subsection was completed, the boundaries were moved to close the
rectangle and to ensure it did not overlap the next selected
subsection. After closing the first subsection, the next subsection
was illuminated, of equal size to the first. The field stop was
used to alternate the position of the rectangle between three
subsections that were sequentially interrogated throughout the
experiment.
[0110] Vapor Generation and Delivery. The fiber was positioned in
the optical path with its face perpendicular to a sparge line, with
a slight modification to the vacuum-controlled sparge setup
described previously in White, J.; Kauer, J. S.; Dickinson, T. A.;
Walt, D. R. Anal. Chem. 1996, 68, 2191-2202 ("White"). Unlike the
previous setup, in which sensors were exposed to their current
environment before and after the vapor pulse, the newly designed
setup continuously delivered ultra pure nitrogen to the sensors
between the vapor pulses. The ultra high purity nitrogen stream
(set to constant 200 mL/min flow rate) and the line dedicated to
the vapor delivery were directed into the common sparge line. A
solenoid trigger was used to alternate between the delivery of
nitrogen and the vapor pulses. Vapors were prepared using an
eight-channel automated vapor delivery system GDS8 (Sensor Research
and Development (SRD), Orono, Me.). The system was comprised of
airtight bubblers, placed in a temperature-controlled oven set at
25.degree. C. to ensure vapor pressures and concentrations of
analytes remained constant throughout the experiment. The hardware,
including solenoid valve switches, mass flow adjustment and
readout, as well as programmed automated delivery of a series of
vapors, were controlled by software custom-designed by SRD.
[0111] Data Collection. Vapor response patterns were collected by
recording fluorescence intensity of the sensors before, during, and
after the vapor pulse, as described previously in White, which is
discussed above. Each response to a vapor, termed vapor exposure,
was recorded in a 10-frame movie (each frame was acquired at 100-ms
exposure time): 2 baseline frames, followed by 5 frames recorded
during the vapor pulse, and 3 frames that recorded the fluorescence
intensity after the vapor pulse. All responses collected in this
study were 2.5 seconds long and included a 1.3-second vapor pulse.
Vapors (50% saturated in air and saturated water vapor) were
delivered to the sensor array at 200 mL/min. The vapor generation
and response collection were fully automated by integrating the
iPLab imaging software (Scanalytics, Fairfax, Va.) with the
custom-designed vapor delivery software from SRD.
[0112] Array Exposure on Subsections. Two different types of
exposures were collected in the study of array longevity: a 2.5-s
vapor exposure and a 2.5-s blank exposure. After every vapor
exposure, the gas delivery lines were purged with an air stream at
200 mL/min flow for 60 s; air was directed to waste during the
first 45 s and to the sensors during the following 15 s. A blank
exposure consisted of the same 2.5-s light exposure but did not
involve a vapor pulse. Every blank exposure was followed by a 10-s
pause to allow partial recovery of dye intensities from
photobleaching. The data from blank exposures were not saved, since
they only served to simulate exposure of the array to excitation
light for studying photobleaching effects on sensors.
[0113] Array Registration. Since arrays contain
randomly-distributed sensors, each array must be decoded in a
process called array registration (See Dickinson, T. A.; Michael,
K. L.; Kauer, J. S.; Walt, D, R. Anal. Chem., 1999; Vol. 71, pp
2192-2198, and Stitzel (discussed above)). Each new randomized
array was masked by a 2.0 ND filter and the sensors were exposed to
three vapors (dimethyl methylphosphonate (DMMP), ethanol, and
heptane) in triplicate. The vapors were pulsed to the array in a
random sequence that had been entered into the vapor delivery
software, and their patterns were stored for registration purposes.
The 30-frame movies acquired for the registration consisted of 4
baseline frames, 8 frames collected during the vapor pulse, and 18
additional frames collected after the pulse. 50% saturated DMMP
vapor responses were sufficiently diverse to register the locations
of the sensor beads. The DMMP vapor patterns (acquired with a
randomized array) were compared with the DMMP patterns stored in a
database. A normalized DMMP pattern response of each of the four
sensor types in the database was obtained by averaging the three
replicates of normalized and averaged single responses of 50 beads.
The beads in a randomized array were decoded by comparing each
normalized DMMP response of an unknown bead to the authentic DMMP
responses of the individual bead types stored in the database. The
registration process was carried out as described previously in
Stitzel using custom-designed software. Although only DMMP
responses were used in the registration process, the remaining
vapor responses contained additional information that was used to
validate the registration accuracy. After the bead decoding was
complete, the ethanol and heptane responses of the registered beads
were averaged by sensor type and compared to the responses of the
individual sensor types in the database. A visual assessment of
similarities between the responses ensured that the registration
decoded the beads correctly.
[0114] Data Analysis. After the sensor positions were registered,
custom-designed data processing software (Department of Computer
Science, Tufts University) was used to normalize and average the
responses of the selected sensors to each vapor by sensor type.
Normalization involves taking the intensities of each vapor
response, subtracting the initial background level from all the
intensity values and dividing the intensities by the highest signal
for that particular response In this way, all vapor responses have
normalized intensities between one and minus one. This procedure is
employed to avoid the use of absolute intensities and to provide
vapor response profiles that are virtually identical in shape for a
given sensor bead type. The responses of all the identical sensors
in a particular subsection were combined and averaged to provide an
averaged response for a vapor pulse. Each vapor exposure therefore
consisted of four averaged responses for the four sensor types
represented per array subsection. The averaged response patterns
were visualized in principal component (PC) space using MATLAB
software that compressed response descriptors by singular value
decomposition (SVD) as described in Albert, K. J.; Walt, D. R.
Anal. Chem. 2003, 75, 4161-4167 ("Albert 1"). Classification scores
were calculated using a k-nearest neighbor (KNN) classifier (k=1)
as described in Cover, T. M.; Hart, P. E. IEEE Transactions on
Information Theory 1967, 13, 21-27 ("Cover"), comprised in Weka 3
Data Mining Software (University of Waikato, New Zealand).
[0115] Composition of Sensor Array. The optical sensor array was
fabricated by randomly distributing thousands of microbead sensors
into etched wells on the end of an optical imaging fiber. The array
contained around 24,000 sensor beads randomly distributed into
etched wells on the end of an optical fiber bundle, as described in
Dickinson, T. A.; Michael, K. L.; Kauer, J. S.; Walt, D. R. Anal.
Chem., 1999; Vol. 71, pp 2192-2198. In this example, four different
microbead sensor types were included giving an average of 6,000
replicates of each sensor type in the array.
[0116] Array Subdivision. An adjustable rectangular field stop
enabled the interrogation of subsections of the sensor array. The
array was subdivided similarly to the method used in our
preliminary report (see Bencic, S.; Walt, D. R. Proc. SPIE-Intl.
Soc. Opt. Eng. 2004, 5269, 83-88 ("Bencic"), where the illumination
was restricted to equal-sized subsections by moving a 50-.mu.m
optical slit across the proximal fiber face. In the present
approach, a rectangular subsection selected by the field stop
restricts the incoming light to a rectangle as shown in FIG. 2. The
rectangle illuminated on the proximal fiber face is then
transmitted across the arrayed fibers to a subsection of microbead
sensors on the distal face. The random nature of the array ensures
that every sensor type is represented by many replicates in each
subsection of the array. When a new array is first connected to the
imaging system, the entire array is viewed for sensor registration.
This "map" of the sensor locations is stored so that when the slit
is moved, the new sensors in the array's field of view are already
registered. The CCD image of the region of illuminated sensors is
shown on the right hand side in FIG. 2. FIG. 3 shows CCD images of
an array with three subsections, each containing a statistical
distribution of microbead sensors, at various times during the
longevity experiment. The entire fiber is observed at the beginning
of the longevity experiment (A), then the three subsections of
sensors are selected (marked with different colors in B). After
repeated exposure to excitation light, the subsections appear
photobleached (C).
[0117] The example presented here was designed to extend array
longevity and assess the performance of the sensor array for vapor
recognition and identification over an extended period of time. The
array was tested for up to 1,800 exposures each day for 14 days.
The testing was interrupted at night, with the array kept in
complete darkness and with a continuous exposure to a stream of
ultra high purity nitrogen. The ambient temperature and relative
humidity ranged between 19.8.degree.-30.degree. C. and 14-21%,
respectively
[0118] Seven saturated vapors (DMMP, ethanol, heptane, isopropenyl
acetate, p-cymene, toluene, and water) were mixed with either 50%
air or 50% water vapor (except for the saturated water vapor) and
the resulting 13 vapor mixtures were delivered to the array at
various times throughout the experiment. The 7 vapor-air mixtures
were used to assess the reproducibility of responses and determine
array longevity, while the remaining 6 vapor-water mixtures were
used to address an additional vapor classification query and are
not discussed in this paper. The 13 vapors were pulsed to the array
in duplicate, which resulted in sets of 26 vapor exposures acquired
at a time. Because vapor preparation and purging are
time-consuming, a number of blank exposures were acquired in
addition to the vapor exposure sets to expose the array to
additional excitation light. Each subsection of the array was
exposed at least 300 times (26 2.5-s vapor exposures followed by
274 2.5-s blank exposures) at each ND setting.
[0119] The terms vapor exposure and blank exposure will be used
herein when referring to a vapor response and an array exposure
without a vapor pulse, respectively. Table 2 summarizes in detail
the number of exposures that were acquired at each ND setting.
TABLE-US-00002 TABLE 2 OD values with corresponding transmission
values (% T) for the ND settings used in the experiment with the
number of exposure sets applied at each ND setting on the three
array subsections. (v--vapor exposure, b--blank exposure,
S--subsection) Sub- Total/ Total/ OD % T sections # Sets #
Exposures Section Array 2 1.0 S2, S3* 1 26v + 274b 300 600* 1.8 1.6
S1-S3 1 26v + 274b 300 900 1.6 2.5 S1-S3 1 26v + 274b 300 900 1.4
4.0 S1-S3 1 26v + 274b 300 900 1.2 6.3 S1-S3 4 26v + 274b 1200 3600
1 300b 300 900 1 10.0 S1-S3 1 26v + 274b 300 900 9 300b 2700 8100 1
274b + 26v 300 900 Sum 20 260v + 5740b 6000 17700 *responses
acquired on S1 at ND 2.0 were part of sensor preconditioning and
were discarded
[0120] Exposure of the array at each ND setting started with the
first subsection at the top left corner of the field of view and
continued with the sections below. In preliminary studies, the same
types of sensors showed that if a fixed subsection was exposed to
increasing light power (a consequence of increasing transmittance
due to removal of the ND filters) significant photobleaching of the
surrounding areas occurred due to light leakage to fibers on
adjacent unexposed regions. To avoid such photobleaching, we
sequentially exposed all three subsections at a high ND setting
before acquiring data with the next lower ND setting. The
transmitted incoming light was only 1% at 2.0 ND and increased in
small increments at the initial ND settings. As the transmission
increased more drastically at the two lowest ND settings used (see
% T values in Table 2), more exposures were acquired at these lower
settings. A total of 754 vapor exposures (vapor-air and
vapor-water) and 16,946 blank exposures were acquired between 1-10%
transmitted light on the three subsections. The 406 vapor exposures
(vapor-air mixtures only) were used to evaluate response
reproducibility.
[0121] Results
[0122] The three subsections represented less than 8% of the total
array surface area and were subjected to 17,700 (2.5-s) exposures,
more than 90-fold (737.5 min vs. 8 min) total exposure time
increase over the arrays reported previously. If the same
experimental scheme were applied to 40 array subsections (as
determined by counting the approximate number of sections projected
onto the entire array surface area) and if the same six ND filter
settings were used, the resulting 236,000 exposures would represent
more than a 2,000-fold improvement over previous arrays (See
Stitzel; and Albert, K. J.; Walt, D. R.; Gill, D. S.; Pearce, T. C.
Anal. Chem. 2001, 73, 2501-2508 ("Albert 2")). The number of
exposures would increase further if higher intensity light was used
by increasing transmission (above 10%). The example described here
therefore represents only a fraction of the full array longevity
that may be achieved with adaptive exposure.
[0123] Unsupervised pattern recognition techniques, such as
singular value decomposition (SVD), classify and group vapor
responses based on a reduced number of response features, rather
than on entire responses. The response features, also referred to
as principal components (PCs), are obtained by compression of the
higher-dimensional response time series (See Otto, M. Chemometrics:
Statistics and Computer Application in Analytical Chemistry;
Wiley-VCH: Weinheim, Federal Republic of Germany, 1999). The first
three PCs obtained by SVD were used for a three-dimensional
visualization of response reproducibility that confirmed the
similarities between vapor patterns collected during the 14-day
experiment.
[0124] FIG. 4 contains plots of fluorescence patterns (a, c, and e)
and the respective SVD response plots (b, d, and f) obtained with
three of the four sensor types in the array (Alitech, Chirex, and
Snafl). These responses were acquired with the three subsections,
each containing a representative number of microbead replicates of
each sensor type. Each plot represents responses to three different
vapors (52 responses acquired with each vapor) obtained with each
sensor type throughout the entire experiment. Only three vapors per
sensor type are presented because smaller sets of response patterns
are easily visualized in a two-dimensional response plot. Although
the vapor responses were collected with sensors from different
array subsections, most of the response shapes were reproducible.
While some of the individual bead sensors gave somewhat spurious
responses, it is important to note that the signals from all bead
replicates in a given subsection are summed before being processed,
thereby providing highly reproducible signals. Moreover, groups of
responses corresponding to specific vapors shown in the
fluorescence response plots formed closely fitting clusters in PC
space, confirming the reproducibility of vapor patterns collected
during the 14 days.
[0125] The numeric assessment of reproducibility between individual
sections of the array and the array as a whole was performed by
calculating classification scores using KNN classification. Our
previous studies have shown that classifiers based on the nearest
neighbors approach are appropriate for the analysis of vapor
response patterns acquired with the optical nose platform (See
Stitzel, discussed above). KNN classification employs part of the
data to compute a model that is used to classify the remaining
data. The KNN classifier finds a nearest neighbor to an unknown
vapor pattern based on the smallest Euclidean distance (See Cover,
discussed above) and assigns the unknown to its nearest neighbor's
class. In this study, a 1-nearest neighbor classifier first built
models based on n-fold leave-one-out cross-validation (n=number of
responses in each data set). In brief, each of the n models
computed from the all except one response were used to classify the
response that was left out.
[0126] Since our goal was not to determine the classification
accuracy for large databases of vapors but to assess the
reproducibility of vapor patterns collected at different points in
time, we compared classification scores for 7 vapors with
concatenated sensor responses (See Stitzel). The scores calculated
for the 7-vapor classification are listed in Table 3.
TABLE-US-00003 TABLE 3 k-nearest neighbor (k = 1) scores calculated
using n-fold cross-validation (n = number of responses) for the
classification of 7 vapors. Subsection 1 2 3 1-3 % Correct 95.2
95.7 95.7 99.8
[0127] KNN computed models from data acquired with a single
subsection (S1, S2, or S3) and with all subsections of sensors
(S1-S3); the response reproducibility was thereby cross-evaluated
within larger groups of vapor responses. The first set of responses
acquired at the first ND setting with sensors from section S1 was
discarded as the sensors were not yet preconditioned (see
Supporting Information). The high classification accuracies
(95.2-99.8%) listed in Table 3 confirmed that array responses to 7
vapors acquired with the three subsections were extremely
reproducible. The accuracy calculated for S1 was a consequence of 6
incorrectly classified vapors out of 126 vapor responses.
Similarly, 6 mistakes out of 140 responses resulted in the
accuracies obtained with sensors on subsections S2 and S3. The
highest classification score (99.8% correct) was obtained when data
from all three sections (S1-S3) were used to build a KNN model.
[0128] Table 4 is a confusion matrix that was calculated for a
7-vapor classification using the model obtained from all data
(S1-S3).
TABLE-US-00004 TABLE 4 Confusion matrix obtained for the 7-vapor
classification with 406 concatenated sensor responses acquired
throughout the entire study with vapor-air mixtures. Classified As
tol DMMP etoh hept pcym ipea water Actual tol 58 0 0 0 0 0 0
Compound DMMP 0 58 0 0 0 0 0 etoh 0 0 57 0 0 1 0 hept 0 0 0 58 0 0
0 pcym 0 0 0 0 58 0 0 ipea 0 0 0 0 0 58 0 water 0 0 0 0 0 0 58
Legend: tol--toluene, DMMP--dimethyl methylphosphonate,
etoh--ethanol, hept--heptane, ipea--isopropenyl acetate,
pcym--p-cymene.
[0129] The values displayed on the diagonal of the confusion matrix
correspond to the number of occurrences when each vapor was
classified correctly. Only one classification mistake was made in
the entire longevity experiment. A response to ethanol was
classified as an isopropenyl acetate response; such classification
error was predictable as these two polar vapors have a similar
effect on the solvatochromism of the dyes attached to the microbead
sensors. Considering that the misclassified vapor response was
acquired on the 6.sup.th day and not at the end of the experiment,
the deviation in the ethanol response pattern was most likely not a
consequence of changes in performance due to the extended sensor
usage.
[0130] The KNN classification scores obtained for the individual
subsections were lower than the nearly 100% accuracy obtained using
data from all the subsections. A classifier trained with three
different subsections of sensors should in theory introduce
array-to-array variability and, as a consequence, the
classification accuracy would be expected to decrease. We surmise
that the accuracy improved because the KNN model, created with the
entire data set, contained a higher population (which can improve a
classifier) and higher variability of responses within each vapor
class. The responses were collected with three different groups of
sensors at various times throughout a two-week experiment. The high
response variability in each vapor class likely increased the
probability for a classified response and its nearest neighbor to
belong to the same vapor class. Training of future vapor
classifiers, especially ones that are intended for identification
of vapor responses acquired over months or years at variable
ambient conditions, should therefore require high representation of
vapor responses acquired with many subgroups of sensors (or with
many arrays) over longer periods of time.
[0131] Discussion
[0132] Longevity of fluorescent microbead sensors was extended by
subdividing the array, and by gradually increasing the illumination
intensity, thereby mitigating sensor photobleaching effects. These
two improvements resulted in a 90-fold increase in the time during
which a sensor array responded reproducibly to a diverse group of
vapors. The slight differences of the sensor responses acquired
over time did not prevent the four sensor types from solving a
seven analyte classification query during the entire array usage.
The vapor responses were reproducible even after the subdivided
array was subjected to 17,700 2.5-s light exposures. Extrapolating
these results to the entire array, 40 subsections could be
interrogated with continuous light exposure for over 160 hours,
representing a significant improvement in array longevity compared
to the previously reported 8 minute exposure time in Stitzel. While
we used 2.5-s exposures in this study, it has previously been
demonstrated that sub-second exposure is adequate for identifying
subgroups of vapors (See Albert, K. J.; Walt, D. R. Anal. Chem.
2000, 72, 1947-1955). If a 2.5-s exposure was collected every
minute, the projected longevity of a single array would be over 160
days and could be extended further if sub-second exposures were
used. Moreover, the longevity would be on the order of years if the
adaptive illumination utilized the entire transmission range.
[0133] Further, the high classification scores, which varied by
bead type, confirmed that the extension of array usage is feasible
by controlling excitation light and exposing smaller sections of
the array. The array longevity-extending approach presented here
introduced a 90-fold increase in the exposure time to which an
array of fluorescence-based sensors can be subjected. This approach
potentially provides a new technique for continuous monitoring not
only with vapor-sensitive sensors but also enzyme-, cell-based, and
other fluorescence-based sensors incorporated into high-density
optical array systems.
[0134] In addition, the experiment was conducted over several weeks
at variable humidities and temperatures, thereby suggesting that
despite the poor day-to-day reproducibility often observed with
sensors, the vapor responses remained comparable. To demonstrate
our method, we used a limited set of sensor types and analytes,
although the same approach may be applied to larger analyte data
sets containing more sensor classes. Our laboratory is developing
new approaches, focused towards diversifying and increasing
database information and enabling its use for more complicated
recognition problems. Both the collection of such large databases
and long-term testing will benefit from this study.
[0135] Although the present invention has been described with
reference to certain embodiments, persons skilled in the art will
recognize that changes may be made in form and detail without
departing from the spirit and scope of the invention.
* * * * *