U.S. patent application number 12/585029 was filed with the patent office on 2009-12-31 for new oxabispidine compunds useful in the treatment of cardiac arrhythmias.
This patent application is currently assigned to AstraZeneca AB. Invention is credited to Annika Bjore, Magnus Bjorsne, David Cladingboel, Kurt-Jurgen Hoffman, John Pavey, Fritiof Ponten, Gert Strandlund, Peder Svensson, Colin Thomson, Michael Wilsterman.
Application Number | 20090326221 12/585029 |
Document ID | / |
Family ID | 20417401 |
Filed Date | 2009-12-31 |
United States Patent
Application |
20090326221 |
Kind Code |
A1 |
Bjore; Annika ; et
al. |
December 31, 2009 |
New oxabispidine compunds useful in the treatment of cardiac
arrhythmias
Abstract
There is provided compounds of formula I, ##STR00001## wherein
R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.41 to R.sup.46, A, B and
G have meanings given in the description, which are useful in the
prophylaxis and in the treatment of arrhythmias, in particular
atrial and ventricular arrhythmias.
Inventors: |
Bjore; Annika; (Stenungsund,
SE) ; Bjorsne; Magnus; (Vastra Frolunda, SE) ;
Cladingboel; David; (Mountsorrel, GB) ; Hoffman;
Kurt-Jurgen; (Kullavik, SE) ; Pavey; John;
(Loughborough, GB) ; Ponten; Fritiof; (Askim,
SE) ; Strandlund; Gert; (Lindome, SE) ;
Svensson; Peder; (Goteborg, SE) ; Thomson; Colin;
(Loughborough Leics, GB) ; Wilsterman; Michael;
(Molndal, SE) |
Correspondence
Address: |
NIXON & VANDERHYE, PC
901 NORTH GLEBE ROAD, 11TH FLOOR
ARLINGTON
VA
22203
US
|
Assignee: |
AstraZeneca AB,
Sodertalje
SE
|
Family ID: |
20417401 |
Appl. No.: |
12/585029 |
Filed: |
September 1, 2009 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11385833 |
Mar 22, 2006 |
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12585029 |
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10317195 |
Dec 12, 2002 |
7164017 |
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11385833 |
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09688251 |
Oct 16, 2000 |
6559143 |
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10317195 |
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Current U.S.
Class: |
544/74 |
Current CPC
Class: |
C07D 498/08 20130101;
A61P 9/00 20180101; A61P 9/06 20180101 |
Class at
Publication: |
544/74 |
International
Class: |
C07D 498/08 20060101
C07D498/08 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 18, 1999 |
SE |
9903759-0 |
Claims
1-45. (canceled)
46. A compound of formula II, ##STR00046## wherein: R.sup.2
represents H, halo, C.sub.1-6 alkyl, --OR.sup.13,
-E-N(R.sup.14)R.sup.15 or, together with R.sup.3, represents
.dbd.O; R.sup.3 represents H, C.sub.1-6 alkyl or, together with
R.sup.2, represents .dbd.O; R.sup.13 represents H, C.sub.1-6 alkyl,
-E-aryl, -E-Het.sup.6, --C(O)R.sup.16a, --C(O)OR.sup.16b or
--C(O)N(R.sup.17a)R.sup.17b; R.sup.14 represents H, C.sub.1-6
alkyl, -E-aryl, -E-Het.sup.6, --C(O)R.sup.16a, --C(O)OR.sup.16b,
--S(O).sub.2R.sup.16c, --[C(O)].sub.pN(R.sup.17a)R.sup.17b or
--C(NH)NH.sub.2; R.sup.15 represents H, C.sub.1-6 alkyl -E-aryl or
--C(O)R.sup.16d; R.sup.16a to R.sup.16d independently represent, at
each occurrence when used herein, C.sub.1-6 alkyl (optionally
substituted and/or terminated by one or more substituents selected
from halo, aryl and Het.sup.7), aryl, Het.sup.8, or R.sup.16a and
R.sup.16d independently represent H; R.sup.17a and R.sup.17b
independently represent, at each occurrence when used herein, H or
C.sub.1-6 alkyl (optionally substituted and/or terminated by one or
more substituents selected from halo, aryl and Het.sup.9), aryl,
Het.sup.10, or together represent C.sub.3-6 alkylene, optionally
interrupted by an O atom; E represents, at each occurrence when
used herein, a direct bond or C.sub.1-4 alkylene; p represents 1 or
2; Het.sup.6 to Het.sup.10 independently represent five- to
twelve-membered heterocyclic groups containing one or more
heteroatoms selected from oxygen, nitrogen and/or sulfur, which
groups are optionally substituted by one or more substituents
selected from --OH, oxo, halo, cyano, nitro, C.sub.1-6 alkyl,
C.sub.1-6 alkoxy, aryl aryloxy, N(R.sup.18a)R.sup.18b,
--C(O)R.sup.18c, --C(O)OR.sup.18d, --C(O)N(R.sup.18e)R.sup.18f,
--N(R.sup.18g)C(O)R.sup.18h and --N(R.sup.18i)S(O).sub.2R.sup.18j;
R.sup.18a to R.sup.18j independently represent C.sub.1-6 alkyl,
aryl or R.sup.18a to R.sup.18j independently represent H; A
represents a direct bond, -J-, -J-N(R.sup.19)-- or -J-O-- (in which
latter two groups, N(R.sup.19)-- or O-- is attached to the carbon
atom bearing R.sup.2 and R.sup.3); B represents -Z-,
-Z-N(R.sup.20)--, --N(R.sup.20)-Z-, -Z-S(O).sub.n--, -Z-O-- (in
which latter two groups, Z is attached to the carbon atom bearing
R.sup.2 and R.sup.3), --N(R.sup.20)C(O)O-Z-, (in which latter
group, --N(R.sup.20) is attached to the carbon atom bearing R.sup.2
and R.sup.3) or --C(O)N(R.sup.20)-- (in which latter group, --C(O)
is attached to the carbon atom bearing R.sup.2 and R.sup.3); J
represents C.sub.1-6 alkylene optionally substituted by one or more
substituents selected from --OH, halo and amino; Z represents a
direct bond or C.sub.1-4 alkylene; n represents 0, 1 or 2; R.sup.19
and R.sup.20 independently represent H or C.sub.1-6 alkyl G
represents CH or N; R.sup.4 represents one or more optional
substituents selected from --OH, cyano, halo, nitro, C.sub.1-6
alkyl (optionally terminated by --N(H)C(O)OR.sup.21a), C.sub.1-6
alkoxy, --N(R.sup.22a)R.sup.22b, --C(O)R.sup.22c, --C(O)OR.sup.22d,
C(O)N(R.sup.22e)R.sup.22f, --N(R.sup.22g)C(O)R.sup.22h,
--N(R.sup.22i)C(O)N(R.sup.22j)R.sup.22k,
--N(R.sup.22m)S(O.sub.2)R.sup.21b, --S(O).sub.2R.sup.21c, and/or
--OS(O).sub.2R.sup.21d; R.sup.21a to R.sup.21d independently
represent C.sub.1-6 alkyl; R.sup.22a and R.sup.22b independently
represent H, C.sub.1-6 alkyl or together represent C.sub.3-6
alkylene, resulting in a four- to seven-membered
nitrogen-containing ring; R.sup.22c or R.sup.22m independently
represent H or C.sub.1-6 alkyl; and wherein each aryl and aryloxy
group, unless otherwise specified, is optionally substituted;
R.sup.41 to R.sup.46 independently represent H or C.sub.1-3 alkyl;
or a protected derivative thereof, optionally in the form of a salt
and/or a solvate.
47. A compound as claimed in claim 46, wherein R.sup.41 to R.sup.46
all represent H; G represents CH; A represents a direct bond; B
represents a direct bond; R.sup.2 represents H or C.sub.1-6 alkyl;
R.sup.3 represents H or C.sub.1-6 alkyl; and/or R.sup.4 is absent
or represents one to three halo, methyl, methoxy or nitro
groups.
48. A compound as claimed in claim 47, wherein R.sup.2 and R.sup.3
both represent H, and R.sup.4 is absent.
49. A compound as claimed in claim 46, wherein the salt is a
hydrochloride, sulfate, or hemisulfate salt.
50. A compound as claimed in claim 49, wherein the salt is a
dihydrochloride.
51. A compound as claimed in claim 50 wherein the salt is a
dihydrochloride hydrate.
52. A compound as claimed in claim 51 wherein the hydrate is a
hemihydrate.
53-75. (canceled)
Description
FIELD OF THE INVENTION
[0001] This invention relates to novel pharmaceutically useful
compounds, in particular compounds which are useful in the
treatment of cardiac arrhythmias.
BACKGROUND AND PRIOR ART
[0002] Cardiac arrhythmias may be defined as abnormalities in the
rate, regularity, or site of origin of the cardiac impulse or as
disturbances in conduction which causes an abnormal sequence of
activation. Arrhythmias may be classified clinically by means of
the presumed site of origin (i.e. as supraventricular, including
atrial and atrioventricular, arrhythmias and ventricular
arrhythmias) and/or by means of rate (i.e. bradyarrhythmias (slow)
and tachyarrhythmias (fast)).
[0003] In the treatment of cardiac arrhythmias, the negative
outcome in clinical trials (see, for example, the outcome of the
Cardiac Arrhythmia Suppression Trial (CAST) reported in New
England. Journal of Medicine, 321, 406 (1989)) with "traditional"
antiarrhythmic drugs, which act primarily by slowing the conduction
velocity (class I antiarrhythmic drugs), has prompted drug
development towards compounds which selectively delay cardiac
repolarization, thus prolonging the QT interval. Class III
antiarrhythmic drugs may be defined as drugs which prolong the
trans-membrane action potential duration (which can be caused by a
block of outward K.sup.+ currents or from an increase of inward ion
currents) and refractoriness, without affecting cardiac
conduction.
[0004] One of the key disadvantages of hitherto known drugs which
act by delaying repolarization (class III or otherwise) is that
they all are known to exhibit a unique form of proarrhythmia known
as torsades de pointes (turning of points), which may, on occasion
be fatal. From the point of view of safety, the minimisation of
this phenomenon (which has also been shown to be exhibited as a
result of administration of non-cardiac drugs such as
phenothiazines, tricyclic antidepressants, antihistamines and
antibiotics) is a key problem to be solved in the provision of
effective antiarrhythmic drugs.
[0005] Antiarrhythmic drugs based on bispidines
(3,7-diazabicyclo[3.3.1]nonanes), are known from inter alia
international patent applications WO 91/07405 and WO 99/31100,
European patent applications 306 871, 308 843 and 655 228 and U.S.
Pat. Nos. 3,962,449, 4,556,662, 4,550,112, 4,459,301 and 5,468,858,
as well as journal articles including inter alia J. Med. Chem. 39,
2559, (1996), Pharmacol. Res., 24, 149 (1991), Circulation, 90,
2032 (1994) and Anal. Sci. 9, 429, (1993). Oxabispidine compounds
are neither disclosed nor suggested in any of these documents.
[0006] Certain oxabispidine compounds are disclosed as chemical
curiosities in Chem. Ber., 96, 2872 (1963). That these compounds
may be used in the treatment of arrhythmias is neither mentioned
nor suggested.
[0007] We have surprisingly found that a novel group of
oxabispidine-based compounds exhibit electrophysiolocical activity,
preferably class III electrophysiological activity, and are
therefore expected to be useful in the treatment of cardiac
arrhythmias.
DISCLOSURE OF THE INVENTION
[0008] According to the invention there is provided compounds of
formula I,
##STR00002##
wherein R.sup.1 represents C.sub.1-12 alkyl (which alkyl group is
optionally substituted and/or terminated by one or more groups
selected from halo, cyano, nitro, aryl, Het.sup.1, C(O)R.sup.5a,
OR.sup.5b, --N(R.sup.6)R.sup.5c, --C(O)XR.sup.7,
--C(O)N(R.sup.8)R.sup.5d, and --S(O).sub.2R.sup.9), or R.sup.1
represents --C(O)XR.sup.7, --C(O)N(R.sup.8)R.sup.5d or
--S(O).sub.2R.sup.9; R.sup.5a to R.sup.5d independently represent,
at each occurrence, H, C.sub.1-6 alkyl (which latter group is
optionally substituted and/or terminated by one or more
substituents selected from --OH, halo, cyano, nitro, aryl and
Het.sup.2), aryl or Het.sup.3, or R.sup.5d, together with R.sup.8,
represents C.sub.3-6 alkylene (which alkylene group is optionally
interrupted by an O atom and/or is optionally substituted by one or
more C.sub.1-3 alkyl groups); R.sup.6 represents H, C.sub.1-6 alkyl
(optionally substituted and/or terminated by one or more
substituents selected from --OH, halo, cyano, nitro and aryl), aryl
--C(O)R.sup.10a, --C(O)OR.sup.10b or --C(O)N(H)R.sup.10c;
R.sup.10a, R.sup.10b and R.sup.10c independently represent
C.sub.1-6 alkyl (optionally substituted and/or terminated by one or
more substituents selected from --OH, halo, cyano, nitro and aryl),
aryl, or R.sup.10a represents H; R.sup.7 represents C.sub.1-12
alkyl (optionally substituted and/or terminated by one or more
substituents selected from --OH, halo, cyano, nitro, aryl,
C.sub.1-6 alkoxy and Het.sup.4); R.sup.8 represents H, C.sub.1-12
alkyl, C.sub.1-6 alkoxy (which latter two groups are optionally
substituted and/or terminated by one or more substituents selected
from --OH, halo, cyano, nitro, C.sub.1-4 alkyl and C.sub.1-4
alkoxy), -D-aryl, -D-aryloxy, -D-Het.sup.5, -D-N(H)C(O)R.sup.11a,
-D-S(O).sub.2R.sup.12a, -D-C(O)R.sup.11b, -D-C(O)OR.sup.12b, -D
C(O)N(R.sup.11c)R.sup.11d, or R.sup.8, together with R.sup.5d,
represents C.sub.3-6 alkylene (which alkylene group is optionally
interrupted by an O atom and/or is optionally substituted by one or
more C.sub.1-3 alkyl groups); R.sup.11a to R.sup.11d independently
represent H, C.sub.1-6 alkyl (optionally substituted and/or
terminated by one or more substituents selected from --OH, halo,
cyano, nitro and aryl), aryl, or R.sup.11c and R.sup.11d together
represent C.sub.3-6 alkylene; R.sup.9, R.sup.12a and R.sup.12b
independently represent C.sub.1-6 alkyl (optionally substituted
and/or terminated by one or more substituents selected from --OH,
halo, cyano, nitro and aryl) or aryl; D represents a direct bond or
C.sub.1-6 alkylene; X represents O or S; R.sup.2 represents H,
halo, C.sub.1-6 alkyl, --OR.sup.13, -E-N(R.sup.14)R.sup.15 or,
together with R.sup.3, represents .dbd.O; R.sup.3 represents H,
C.sub.1-6 alkyl or, together with R.sup.2, represents .dbd.O;
R.sup.13 represents H, C.sub.1-6 alkyl, -E-aryl, -E-Het.sup.6,
--C(O)R.sup.16a, C(O)OR.sup.16b or --C(O)N(R.sup.17a)R.sup.17b;
R.sup.14 represents H, C.sub.1-6 alkyl, -E-aryl, -E-Het.sup.6,
--C(O)R.sup.16a, --C(O)OR.sup.16b, --S(O).sub.2R.sup.16c,
[C(O)].sub.pN(R.sup.17a)R.sup.17b or --C(NH)NH.sub.2; R.sup.15
represents H, C.sub.1-6 alkyl, -E-aryl or --C(O)R.sup.16d;
R.sup.16a to R.sup.16d independently represent, at each occurrence
when used herein, C.sub.1-6 alkyl (optionally substituted and/or
terminated by one or more substituents selected from halo, aryl and
Het.sup.7), aryl, Het.sup.8, or R.sup.16a and R.sup.16d
independently represent H; R.sup.17a and R.sup.17b independently
represent, at each occurrence when used herein, H or C.sub.1-6
alkyl (optionally substituted and/or terminated by one or more
substituents selected from halo, aryl and Het.sup.9), aryl,
Het.sup.10, or together represent C.sub.3-6 alkylene, optionally
interrupted by an O atom; E represents, at each occurrence when
used herein, a direct bond or C.sub.1-4 alkylene; p represents 1 or
2; Het.sup.1 to Het.sup.10 independently represent five- to
twelve-membered heterocyclic groups containing one or more
heteroatoms selected from oxygen, nitrogen and/or sulfur, which
groups are optionally substituted by one or more substituents
selected from --OH, oxo, halo, cyano, nitro, C.sub.1-6alkyl,
C.sub.1-6 alkoxy, aryl, aryloxy, --N(R.sup.18a)R.sup.18b,
--C(O)R.sup.18c, --C(O)OR.sup.18d, --C(O)N(R.sup.18e)R.sup.18f,
--N(R.sup.18g)C(O)R.sup.18h and --N(R.sup.18i)S(O).sub.2R.sup.18j;
R.sup.18a to R.sup.18j independently represent C.sub.1-6 alkyl,
aryl or R.sup.18a to R.sup.18i independently represent H; A
represents a direct bond, -J-, -J-N(R.sup.19)-- or -J-O-- (in which
latter two groups, N(R.sup.19)-- or O-- is attached to the carbon
atom bearing R.sup.2 and R.sup.3); B represents -Z-,
-Z-N(R.sup.20)--, --N(R.sup.20)-Z-, -Z-S(O).sub.n--, -Z-O-- (in
which latter two groups, Z is attached to the carbon atom bearing
R.sup.2 and R.sup.3), --N(R.sup.20)C(O)O-Z-, (in which latter
group, --N(R.sup.20) is attached to the carbon atom bearing R.sup.2
and R.sup.3) or --C(O)N(R.sup.20)-- (in which latter group, --C(O)
is attached to the carbon atom bearing R.sup.2 and R.sup.3); J
represents C.sub.1-6 alkylene optionally substituted by one or more
substituents selected from --OH, halo and amino; Z represents a
direct bond or C.sub.1-4 alkylene; n represents 0, 1 or 2; R.sup.19
and R.sup.20 independently represent H or C.sub.1-6 alkyl; G
represents CH or N; R.sup.4 represents one or more optional
substituents selected from --OH, cyano, halo, nitro, C.sub.1-6
alkyl (optionally terminated by --N(H)C(O)OR.sup.21a), C.sub.1-6
alkoxy, --N(R.sup.22a)R.sup.22b, --C(O)R.sup.22c, --C(O)OR.sup.22d,
--C(O)N(R.sup.22e)R.sup.22f, --N(R.sup.22g)C(O)R.sup.22h,
--N(R.sup.22i)C(O)N(R.sup.22j)R.sup.22k,
--N(R.sup.22m)S(O).sub.2R.sup.21b, --S(O).sub.2R.sup.21c, and/or
--OS(O).sub.2R.sup.21d; R.sup.21a to R.sup.21d independently
represent C.sub.1-6 alkyl; R.sup.22a and R.sup.22b independently
represent H, C.sub.1-6 alkyl or together represent C.sub.3-6
alkylene, resulting in a four- to seven-membered
nitrogen-containing ring; R.sup.22c to R.sup.22m independently
represent H or C.sub.1-6 alkyl; and R.sup.41 to R.sup.46
independently represent H or C.sub.1-3 alkyl; wherein each aryl and
aryloxy group, unless otherwise specified, is optionally
substituted; provided that (a) the compound is not: [0009]
3,7-dibenzoyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane; (b) when A
represents -J-N(R.sup.19)-- or -J-O--, then: [0010] (i) J does not
represent C.sub.1 alkylene; and [0011] (ii) B does not represent
--N(R.sup.20)--, --N(R.sup.20)-Z- (in which latter group
N(R.sup.20) is attached to the carbon atom bearing R.sup.2 and
R.sup.3), --S(O).sub.n--, --O-- or --N(R.sup.20)C(O)O-Z- when
R.sup.2 and R.sup.3 do not together represent .dbd.O; and (c) when
R.sup.2 represents --OR.sup.13 or --N(R.sup.14)(R.sup.15), then:
[0012] (i) A does not represent -J-N(R.sup.19)-- or -J-O--; and
[0013] (ii) B does not represent --N(R.sup.20)--, --N(R.sup.20)-Z-
(in which latter group N(R.sup.20) is attached to the carbon atom
bearing R.sup.2 and R.sup.3), --S(O).sub.n--, --O-- or
--N(R.sup.20)C(O)O-Z-; or a pharmaceutically acceptable derivative
thereof; which compounds are referred to hereinafter as "the
compounds of the invention".
[0014] Unless otherwise specified, alkyl groups and alkoxy groups
as defined herein may be straight-chain or, when there is a
sufficient number (i.e. a minimum of three) of carbon atoms be
branched-chain, and/or cyclic. Further, when there is a sufficient
number (i.e. a minimum of four) of carbon atoms, such alkyl and
alkoxy groups may also be part cyclic/acyclic. Such alkyl and
alkoxy groups may also be saturated or, when there is a sufficient
number (i.e. a minimum of two) of carbon atoms, be unsaturated
and/or interrupted by one or more oxygen and/or sulfur atoms.
Unless otherwise specified, alkyl and alkoxy groups may also be
substituted by one or more halo, and especially fluoro, atoms.
[0015] Unless otherwise specified, alkylene groups as defined
herein may be straight-chain or, when there is a sufficient number
(i.e. a minimum of two) of carbon atoms, be branched-chain. Such
alkylene chains may also be saturated or, when there is a
sufficient number (i.e. a minimum of two) of carbon atoms, be
unsaturated and/or interrupted by one or more oxygen and/or sulfur
atoms. Unless otherwise specified, alkylene groups may also be
substituted by one or more halo atoms.
[0016] The term "aryl", when used herein, includes C.sub.6-10 aryl
groups such as phenyl, naphthyl and the like. The term "aryloxy",
when used herein includes C.sub.6-10 aryloxy groups such as
phenoxy, naphthoxy and the like. For the avoidance of doubt,
aryloxy groups referred to herein are attached to the rest of the
molecule via the O-atom of the oxy-group. Unless otherwise
specified, aryl and aryloxy groups may be substituted by one or
more substituents including --OH, halo, cyano, nitro, C.sub.1--
alkyl, C.sub.1-6 alkoxy, N(R.sup.22a)R.sup.22b, --C(O)R.sup.22c,
--C(O)OR.sup.22d, --C(O)N(R.sup.22e)R.sup.22f,
--N(R.sup.22g)C(O)R.sup.22h, --N(R.sup.22m)S(O).sub.2R.sup.21b,
--S(O).sub.2R.sup.21c, and/or --OS(O).sub.2R.sup.21d (wherein
R.sup.21b to R.sup.21d and R.sup.22a to R.sup.22m are as
hereinbefore defined). When substituted, aryl and aryloxy groups
are preferably substituted by between one and three
substitutents.
[0017] The term "halo", when used herein, includes fluoro, chloro,
bromo and iodo.
[0018] Het (Het.sup.1, Het.sup.2, Het.sup.3, Het.sup.4, Het.sup.5,
Het.sup.6, Het.sup.7, Het.sup.8, Het.sup.9 and Het.sup.10) groups
that may be mentioned include those containing 1 to 4 heteroatoms
(selected from the group oxygen, nitrogen and/or sulfur) and in
which the total number of atoms in the ring system are between five
and twelve. Het (Het.sup.1, Het.sup.2, Het.sup.3, Het.sup.4,
Het.sup.5, Het.sup.6, Het.sup.7, Het.sup.8, Het.sup.9 and
Het.sup.10) groups may be fully saturated, wholly aromatic, partly
aromatic and/or bicyclic in character. Heterocyclic groups that may
be mentioned include benzodioxanyl, benzodioxepanyl, benzodioxolyl,
benzofuranyl, benzimidazolyl, benzomorpholinyl, benzoxazinonyl,
benzothiophenyl, chromanyl, cinnolinyl, dioxanyl, furanyl,
imidazolyl, imidazo[1,2-a]pyridinyl, indolyl, isoquinolinyl,
isoxazolyl, morpholinyl, oxazolyl, phthalazinyl, piperazinyl,
piperidinyl, purinyl, pyranyl, pyrazinyl, pyrazolyl, pyridinyl,
pyrimindinyl, pyrrolidinonyl, pyrrolidinyl, pyrrolinyl, pyrrolyl,
quinazolinyl, quinolinyl, tetrahydropyranyl, tetrahydrofuranyl,
thiazolyl, thienyl, thiochromanyl, triazolyl and the like. Values
of Het.sup.1 that may be mentioned include pyridinyl,
benzodioxanyl, imidazolyl, imidazo[1,2-a]pyridinyl, piperazinyl,
pyrazolyl, pyrrolyl, pyrrolidinyl, tetrahydropyranyl and thiazolyl.
Values of Het.sup.3 that may be mentioned include benzodioxanyl and
benzomorpholinyl. Values of Het.sup.4 that may be mentioned include
piperazinyl. Substituents on Het (Het.sup.1, Het.sup.2, Het.sup.3,
Het.sup.4, Het.sup.5, Het.sup.6, Het.sup.7, Het.sup.8, Het.sup.9
and Het.sup.10) groups may, where appropriate, be located on any
atom in the ring system including a heteroatom. The point of
attachment of Het (Het.sup.1, Het.sup.2, Het.sup.3, Het.sup.4,
Het.sup.5, Het.sup.6, Het.sup.7, Het.sup.8, Het.sup.9 and
Het.sup.10) groups may be via any atom in the ring system including
(where appropriate) a heteroatom, or an atom on any fused
carbocyclic ring that may be present as part of the ring system.
Het (Het.sup.1, Het.sup.2, Het.sup.3, Het.sup.4, Het.sup.5,
Het.sup.6, Het.sup.7, Het.sup.8, Het.sup.9 and Het.sup.10) groups
may also be in the N- or S-oxidised form.
[0019] Pharmaceutically acceptable derivatives include salts and
solvates. Salts which may be mentioned include acid addition salts.
Specific salts that may be mentioned include arylsulfonate salts,
such as toluenesulfonate and, especially, benzenesulfonate salts.
Solvates that may be mentioned include hydrates, such as
monohydrates of the compounds of the invention.
[0020] Pharmaceutically acceptable derivatives also include, at the
oxabispidine or (when G represents N) pyridyl nitrogens, C.sub.1-4
alkyl quaternary ammonium salts and N-oxides, provided that when a
N-oxide is present: [0021] (a) no Het (Het.sup.1, Het.sup.2,
Het.sup.3, Het.sup.4, Het.sup.5, Het.sup.6, Het.sup.7, Het.sup.8,
Het.sup.9 and Het.sup.10) group contains an unoxidised S-atom;
and/or [0022] (b) n does not represent 0 when B represents
-Z-S(O).sub.n--.
[0023] The compounds of the invention may exhibit tautomerism. All
tautomeric forms and mixtures thereof are included within the scope
of the invention.
[0024] The compounds of the invention may also contain one or more
asymmetric carbon atoms and may therefore exhibit optical and/or
diastereoisomerism. Diastereoisomers may be separated using
conventional techniques, e.g. chromatography or fractional
crystallisation. The various stereoisomers may be isolated by
separation of a racemic or other mixture of the compounds using
conventional, e.g. fractional crystallisation or HPLC, techniques.
Alternatively the desired optical isomers may be made by reaction
of the appropriate optically active starting materials under
conditions which will not cause racemisation or epimerisation, or
by derivatisation, for example with a homochiral acid followed by
separation of the diastereomeric esters by conventional means (e.g.
HPLC, chromatography over silica). All stereoisomers are included
within the scope of the invention.
[0025] Abbreviations are listed at the end of this
specification.
[0026] Compounds of formula I that may be mentioned include those
in which, when R.sup.2 and R.sup.3 together represent .dbd.O, then
A and B do not simultaneously represent direct bonds.
[0027] Preferred compounds of the invention include those in
which:
R.sup.1 represents C.sub.1-8 alkyl (which alkyl group is optionally
substituted and/or terminated by one or more groups selected from
halo, optionally substituted aryl, optionally substituted
Het.sup.1, --C(O)R.sup.5a, --OR.sup.5b, --N(R.sup.6)R.sup.5c,
--C(O)N(R.sup.8)R.sup.5d, and --S(O).sub.2R.sup.9), or R.sup.1
represents --C(O)OR.sup.7, --C(O)N(R.sup.8)R.sup.5d or
--S(O).sub.2R.sup.9; R.sup.5a to R.sup.5d independently represent,
at each occurrence, H, Clot alkyl (which latter group is optionally
substituted and/or terminated by one or more substituents selected
from halo, cyano, nitro and aryl), aryl (which latter group is
optionally substituted by one or more substituents selected from
halo, hydroxy, cyano, nitro, N(R.sup.22a)R.sup.22b (in which latter
group R.sup.22a and R.sup.22b together represent C.sub.3-6
alkylene), C.sub.1-4 alkyl and C.sub.1-4 alkoxy (which latter two
groups are optionally substituted by one or more halo atoms)),
Het.sup.3, or R.sup.5d, together with R.sup.8, represents C.sub.4-5
alkylene (which alkylene group is optionally interrupted by an O
atom); R.sup.6 represents H, C.sub.1-6 alkyl, aryl (which latter
group is optionally substituted by one or more substituents
selected from halo, cyano, nitro, C.sub.1-4 alkyl and C.sub.1-4
alkoxy), --C(O)R.sup.10a, --C(O)OR.sup.10b or C(O)N(H)R.sup.10c;
R.sup.10a and R.sup.10b independently represent C.sub.1-4 alkyl
(optionally substituted by one or more substituents selected from
halo and aryl) or aryl (which latter group is optionally
substituted by or more substituents selected from halo, cyano,
nitro, C.sub.1-4 alkyl and C.sub.1-4 alkoxy); R.sup.10c represents
C.sub.1-4 alkyl; R.sup.7 represents C.sub.1-6 alkyl optionally
substituted and/or terminated by one or more substituents selected
from halo, aryl, C.sub.1-4 alkoxy and Het.sup.4; R.sup.8 represents
H, C.sub.1-6 alkyl (which latter group is optionally substituted
and/or terminated by one or more substituents selected from halo,
cyano and nitro), -D-aryl, -D-aryloxy, -D-Het.sup.5,
-D-N(H)C(O)R.sup.11a, -D-C(O)R.sup.11b, or R.sup.8, together with
R.sup.5d, represents C.sub.4-5 alkylene (which alkylene group is
optionally interrupted by an O atom); R.sup.11a and R.sup.11b
independently represent C.sub.1-4 alkyl (optionally substituted
and/or terminated by one or more substituents selected from halo,
cyano, nitro and aryl) or aryl; D represents a direct bond or
C.sub.1-3 alkylene; R.sup.9 represents C.sub.1-6 alkyl (optionally
substituted by one or more halo groups) or aryl (which latter group
is optionally substituted by one or more substituents selected from
C.sub.1-4 alkyl, C.sub.1-4 alkoxy, halo, nitro and cyano); R.sup.2
represents H, halo, C.sub.1-3 alkyl, --OR.sup.13, --N(H)R.sup.14
or, together with R.sup.3, represents .dbd.O; R.sup.3 represents H,
C.sub.1-3 alkyl or, together with R.sup.2, represents .dbd.O;
R.sup.13 represents H, C.sub.1-4 alkyl, -E-aryl (optionally
substituted by one or more substituents selected from cyano, halo,
nitro, C.sub.1-4 alkyl and C.sub.1-4 alkoxy), or -E-Het.sup.6;
R.sup.14 represents H, C.sub.1-6 alkyl, -E-aryl (which aryl group
is optionally substituted by one or more substituents selected from
cyano, halo, nitro, C.sub.1-4 alkyl and C.sub.1-4 alkoxy),
--C(O)R.sup.16a, --C(O)OR.sup.16b, --S(O).sub.2R.sup.16c,
--C(O)N(R.sup.17a)R.sup.17b or --C(NH)NH.sub.2; R.sup.16a to
R.sup.16c independently represent C.sub.1-6 alkyl, or R.sup.16a
represents H; R.sup.17a and R.sup.17b independently represent H or
C.sub.1-4 alkyl; E represents a direct bond or C.sub.1-2 alkylene;
Het.sup.1 to Het.sup.6 are optionally substituted by one or more
substituents selected from oxo, halo, cyano, nitro, C.sub.1-4
alkyl, C.sub.1-4 alkoxy, --N(R.sup.18a)R.sup.18b, --C(O)R.sup.18c
or --C(O)OR.sup.18d; R.sup.18a to R.sup.18d independently represent
H, C.sub.1-4 alkyl or aryl; A represents -J-, -J-N(R.sup.19)-- or
-J-O--; B represents -Z-, -Z-N(R.sup.20)--, --N(R.sup.20)-Z-,
-Z-S(O).sub.n--, -Z-O-- or --N(R.sup.20)C(O)O-Z-; J represents
C.sub.1-4 alkylene; Z represents a direct bond or C.sub.1-3
alkylene; n represents 0 or 2; R.sup.19 and R.sup.20 independently
represent H or C.sub.1-4 alkyl; when G represents N, R.sup.4 is in
the ortho- or, in particular, the para-position relative to the
point of attachment of B; when G represents N, R.sup.4 is absent or
represents a single cyano group; R.sup.4 is selected from --OH,
cyano, halo, nitro, C.sub.1-6 alkyl, C.sub.1-6 alkoxy,
--C(O)N(R.sup.22e)R.sup.22f, --N(R.sup.22g)C--(O)R.sup.22h, and/or
N(R.sup.22m)S(O).sub.2--C.sub.1-4 alkyl; R.sup.22e to R.sup.22m
independently represent H or C.sub.1-4 alkyl; R.sup.41 to R.sup.46
independently represent H.
[0028] More preferred compounds of the invention include those in
which:
R.sup.1 represents straight-chain or branched-chain or part
cyclic/acyclic C.sub.1-6 alkyl optionally interrupted by oxygen
and/or optionally substituted and/or terminated by: (i) one or more
halo or --OR.sup.5b groups; and/or (ii) one group selected from
phenyl (which latter group is optionally substituted by one or more
substituents selected from halo, cyano and C.sub.1-4 alkoxy (which
latter group is optionally substituted by one or more halo atoms)),
Het.sup.1, --C(O)R.sup.5a, --N(H)R.sup.6, --C(O)N(R.sup.8)R.sup.5d,
and --S(O).sub.2--C.sub.1-4 alkyl, or R.sup.1 represents
--C(O)OR.sup.7, --C(O)N(R.sup.8)R.sup.5d or --S(O).sub.2--C.sub.1-5
alkyl; Het.sup.1 represents a four- (e.g. five-) to ten-membered
heterocyclic group containing one or two heteroatoms selected from
oxygen, nitrogen and/or sulfur, which group is optionally
substituted by one or more substituents selected from C.sub.1-2
alkyl and --C(O)--C.sub.1-4 alkyl; R.sup.5a, R.sup.5b and R.sup.5d
independently represent H, C.sub.1-5 alkyl, phenyl (which latter
group is optionally substituted by one or more substituents
selected from halo, hydroxy, cyano, pyrrolidinyl, C.sub.1-4 alkyl
and C.sub.1-5 alkoxy (which latter group is optionally substituted
by one or more halo atoms)) or Het.sup.3; Het.sup.3 represents a
five- to ten-membered heterocyclic group containing one or two
heteroatoms selected from oxygen and nitrogen, which group is
optionally substituted by one or more substituents selected from
oxo, C.sub.1-2 alkyl and --C(O)--C.sub.1-4 alkyl; R.sup.6
represents H, C.sub.1-4 alkyl, phenyl (which latter group is
optionally substituted by one or more cyano groups) or
--C(O)O--C.sub.1-5 alkyl; R.sup.7 represents C.sub.1-5 alkyl
optionally substituted or terminated by Het.sup.4; Het.sup.4
represents a five- to ten-membered heterocyclic group containing
one or two heteroatoms selected from oxygen and nitrogen, which
group is optionally substituted by one or more substituents
selected from C.sub.1-2 alkyl and --C(O)--C.sub.1-4 alkyl; R.sup.8
represents H or C.sub.1-4 alkyl; R.sup.2 represents H, --OR.sup.13
or --N(H)R.sup.14; R.sup.3 represents H; R.sup.13 represents H or
phenyl (optionally substituted by one or more substituents selected
from cyano and C.sub.1-2 alkoxy); R.sup.14 represents H, phenyl
(optionally substituted by one or more cyano groups) or
--C(O)O--C.sub.1-5 alkyl; A represents C.sub.1-3 alkylene; B
represents -Z-, -Z-N(H)--, -Z-S(O).sub.2--, or -Z-O-- (in which
latter three groups, Z is attached to the carbon atom bearing
R.sup.2 and R.sup.3); Z represents a direct bond or C.sub.1-2
alkylene; G represents CH; R.sup.4 represents one or two cyano
groups in the ortho- and/or, in particular, the para-position
relative to B.
[0029] Particularly preferred compounds of the invention include
those in which:
R.sup.1 represents straight-chain or branched-chain or part
cyclic/acyclic C.sub.1-6 alkyl optionally interrupted by oxygen
and/or optionally substituted and/or terminated by: (i) one or more
halo or --OR.sup.5b groups; and/or (ii) one group selected from
phenyl (which latter group is optionally substituted by one or more
substituents selected from halo, cyano and C.sub.1-4 alkoxy (which
latter group is optionally substituted by one or more halo atoms)),
Het.sup.1, --C(O)R.sup.5a, --N(H)R.sup.6, --C(O)N(R.sup.8)R.sup.5d,
and --S(O).sub.2--C.sub.1-4 alkyl.
[0030] Especially preferred compounds of the invention include
those in which:
R.sup.1 represents straight- or branched-chain C.sub.1-4 alkyl
(e.g. C.sub.1-3 alkyl) terminated by --C(O)R.sup.5a or
--N(H)C(O)OR.sup.10b: R.sup.5a and R.sup.10b independently
represent straight- or branched-chain C.sub.2-6 alkyl (e.g.
C.sub.3-5 alkyl, such butyl (e.g. t-butyl)); R.sup.2 represents H
or OH; A represents C.sub.1-2 alkylene; B represents -Z-, -Z-N(H)--
or -Z-O-- (in which latter two groups, Z is attached to the carbon
atom bearing R.sup.2 and R.sup.3, and represents C.sub.1-2
alkylene); R.sup.4 is a single cyano group in the para-position
relative to B.
[0031] Preferred compounds of the invention include the compounds
of the Examples disclosed hereinafter.
[0032] Preferred compounds of the invention also include those in
which:
R.sup.6 does not represent --C(O)N(H)R.sup.10c; R.sup.22a and
R.sup.22b do not together represent C.sub.3-6alkylene.
[0033] Preferred compounds of the invention also include those
which are not: [0034] tert-butyl
7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate; or
[0035] ethyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[-
3.3.1]nonane-3-carboxylate;
Preparation
[0036] According to the invention there is also provided a process
for the preparation of compounds of formula I which comprises:
(a) reaction of a compound of formula II,
##STR00003##
wherein R.sup.2, R.sup.3R.sup.4, R.sup.41 to R.sup.46, A, B and G
are as hereinbefore defined, with a compound of formula III,
R.sup.1-L.sup.1 III
wherein L.sup.1 represents a leaving group such as halo,
alkanesulfonate, perfluoroalkanesulfonate, arenesulfonate,
--OC(O)XR.sup.7, imidazole or R.sup.23O-- (wherein R.sup.23
represents, for example, C.sub.1-10 alkyl or aryl, which groups are
optionally substituted by one or more halo or nitro groups) and X,
R.sup.1 and R.sup.7 are as hereinbefore defined, for example at
between room and reflux temperature in the presence of a suitable
base (e.g. triethylamine, potassium carbonate or a bicarbonate,
such as sodium bicarbonate) and an appropriate solvent (e.g.
dichloromethane, chloroform, acetonitrile, N,N-dimethylformamide,
THF, toluene, water, a lower alkyl alcohol (e.g. ethanol) or
mixtures thereof); (b) for compounds of formula I in which R.sup.1
represents --C(O)XR.sup.7 or --C(O)N(R.sup.8)R.sup.5d, reaction of
a compound of formula IV,
##STR00004##
wherein R.sup.2, R.sup.3, R.sup.4, R.sup.41 to R.sup.46, A, B, G
and L.sup.1 are as hereinbefore defined, with a compound of formula
V,
R.sup.24--H V
wherein R.sup.24 represents --XR.sup.7 or --N(R.sup.8)R.sup.5d and
R.sup.5d, R.sup.7, R.sup.8 and X are as hereinbefore defined, for
example under similar conditions to those described hereinbefore
(process step (a)); (c) for compounds in which R.sup.1 represents
--C(O)N(H)R.sup.8, reaction of a compound of formula II, as
hereinbefore defined, with a compound of formula VI,
R.sup.3--N.dbd.C.dbd.O VI
wherein R.sup.3 is as hereinbefore defined, for example at between
0.degree. C. and reflux temperature in the presence of an
appropriate organic solvent (e.g. dichloromethane), or via solid
phase synthesis under conditions known to those skilled in the art;
(d) reaction of a compound of formula VII,
##STR00005##
wherein R.sup.1 and R.sup.41 to R.sup.46 are as hereinbefore
defined, with a compound of formula VIII,
##STR00006##
wherein L.sup.2 represents a leaving group such as halo,
alkanesulfonate (e.g. to mesylate), perfluoroalkanesulfonate or
arenesulfonate (e.g. 2- or 4-nitrobenzenesulfonate,
toluenesulfonate or benzenesulfonate) and R.sup.2, R.sup.3,
R.sup.4, A, B and G are as hereinbefore defined, for example at
elevated temperature (e.g. between 35.degree. C. and reflux
temperature) in the presence of a suitable base (e.g. triethylamine
or potassium carbonate) and an appropriate organic solvent (e.g.
acetonitrile, dichloromethane, chloroform, dimethylsulfoxide,
N,N-dimethylformamide, a lower alkyl alcohol (e.g. ethanol),
isopropyl acetate or mixtures thereof); (e) for compounds of
formula I in which A represents CH.sub.2 and R.sup.2 represents
--OH or --N(H)R.sup.14, reaction of a compound of formula VII, as
hereinbefore defined, with a compound of formula IX,
##STR00007##
wherein Y represents O or N(R.sup.14) and R.sup.3, R.sup.4,
R.sup.14, B and G are as hereinbefore defined, for example at
elevated temperature (e.g. 60.degree. C. to reflux) in the presence
of a suitable solvent (e.g. a lower alkyl alcohol (e.g. IPA),
acetonitrile, or a mixture of a lower alkyl alcohol and water); (f)
for compounds of formula I in which B represents -Z-O--, reaction
of a compound of formula X,
##STR00008##
wherein R.sup.1, R.sup.2, R.sup.3, R.sup.41 to R.sup.46, A and Z
are as hereinbefore defined, with a compound of formula XI,
##STR00009##
wherein R.sup.4 and G are as hereinbefore defined, for example
under Mitsunobu-type conditions e.g. at between ambient (e.g.
25.degree. C.) and reflux temperature in the presence of a tertiary
phosphine (e.g. tributylphosphine or triphenylphosphine), an
azodicarboxylate derivative (e.g. diethylazodicarboxylate or
1,1'-(azodicarbonyl)dipiperidine) and an appropriate organic
solvent (e.g. dichloromethane or toluene); (g) for compounds of
formula I in which G represents N and B represents -Z-O--, reaction
of a compound of formula X, as hereinbefore defined, with a
compound of formula XII,
##STR00010##
wherein R.sup.4 and L.sup.2 are as hereinbefore defined, for
example at between 10.degree. C. and reflux temperature in the
presence of a suitable base (e.g. sodium hydride) and an
appropriate solvent (e.g. N,N-dimethylformamide); (h) for compounds
of formula I in which R.sup.2 represents --OR.sup.13, in which
R.sup.13 represents C.sub.1-6 alkyl, -E-aryl or -E-Het.sup.6,
reaction of a compound of formula I in which R.sup.2 represents OH
with a compound of formula XIII,
R.sup.13aOH XIII
wherein R.sup.13a represents C.sub.1-6 alkyl, -E-aryl or
-E-Het.sup.6 and E and Het.sup.6 are as hereinbefore defined, for
example under Mitsunobu-type conditions (e.g. as described
hereinbefore in process step (f)); (i) for compounds of formula I
in which R.sup.2 represents --OR.sup.13, in which R.sup.13
represents C.sub.1-6 alkyl, -E-aryl or -E-Het.sup.6, reaction of a
compound of formula XIV,
##STR00011##
wherein R.sup.1, R.sup.3, R.sup.4, R.sup.41 to R.sup.46, A, B, G
and L.sup.2 are as hereinbefore defined, with a compound of formula
XIII, as hereinbefore defined, for example at between ambient (e.g.
25.degree. C.) and reflux temperature, under Williamson-type
conditions (i.e. in the presence of an appropriate base (e.g. KOH
or NaH) and a suitable organic solvent (e.g. dimethylsulfoxide or
N,N-dimethylformamide)) (the skilled person will appreciate that
certain compounds of formula XIV (e.g. those in which L.sup.2
represents halo) may also be regarded as compounds of formula I as
hereinbefore defined); (j) for compounds of formula I in which
R.sup.2 represents -E-NH.sub.2, reduction of a compound of formula
XV,
##STR00012##
wherein R.sup.1, R.sup.3, R.sup.4, R.sup.41 to R.sup.46, A, B, E
and G are as hereinbefore defined, for example by hydrogenation at
a suitable pressure in the presence of a suitable catalyst (e.g.
palladium on carbon) and an appropriate solvent (e.g. a
water-ethanol mixture); (k) for compounds of formula I in which
R.sup.2 represents -E-N(R.sup.14)R.sup.15, wherein R.sup.14
represents C.sub.1-6 alkyl, -E-aryl -E-Het.sup.6, --C(O)R.sup.16a,
--C(O)OR.sup.16b, --S(O).sub.2R.sup.16c or
--C(O)N(R.sup.17a)R.sup.17b, reaction of a compound of formula I in
which R.sup.2 represents -E-N(H)R.sup.15 with a compound of formula
XVI,
R.sup.14a-L.sup.1 XVI
wherein R.sup.14a represents C.sub.1-6 alkyl, -E-aryl -E-Het.sup.6,
--C(O)R.sup.16a, --C(O)OR.sup.16b, --S(O).sub.2R.sup.16c or
C(O)N(R.sup.17a)R.sup.17b, and R.sup.16a, R.sup.16b, R.sup.16c,
R.sup.17a, R.sup.17b, Het.sup.6, E and L.sup.1 are as hereinbefore
deemed, for example under conditions described hereinbefore
(process step (a)); (l) for compounds of formula I in which R.sup.2
represents -E-N(R.sup.15)C(O)N(H)R.sup.17a, reaction of a compound
of formula I in which R.sup.2 represents -E-N(H)R.sup.15 with a
compound of formula XVII,
R.sup.17a--N.dbd.C.dbd.O XVII
wherein R.sup.17a is as hereinbefore defined, for example under
conditions described hereinbefore (process step (c)); (m) for
compounds of formula I in which R.sup.2 represents
-E-N(H)[C(O)].sub.2NH.sub.2, reaction of a compound of formula I in
which R.sup.2 represents -E-NH, with oxalic acid diamide, for
example at between -10 and 25.degree. C. in the presence of a
suitable coupling agent (e.g.
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide), an appropriate
activating agent (e.g. 1-hydroxybenzotriazole), a suitable base
(e.g. triethylamine) and a reaction-inert solvent (e.g.
N,N-dimethylformamide); (n) for compounds of formula I in which
R.sup.2 represents -E-N(H)C(NH)NH.sub.2, reaction of a compound of
formula I in which R.sup.2 represents -E-NH.sub.2 with a compound
of formula XVIII,
R.sup.23O--C(.dbd.NH)NH.sub.2 XVIII
or an N-protected derivative thereof, wherein R.sup.23 is as
hereinbefore defined, for example at between room and reflux
temperature, optionally in the presence of a suitable solvent (e.g.
toluene) and/or an appropriate acidic catalyst (e.g. acetic acid
at, for example, 10 mol %); (o) for compounds of formula I in which
R.sup.2 represents --OR.sup.13, in which R.sup.13 represents
--C(O)R.sup.16a, --C(O)OR.sup.16b or --C(O)N(R.sup.17a)R.sup.17b,
reaction of a compound of formula I in which R.sup.2 represents
--OH with a compound of formula XIX,
R.sup.13b-L.sup.3 XIX
wherein R.sup.13b represents --C(O)R.sup.16a, --C(O)OR.sup.16b or
--C(O)N(R.sup.17a)R.sup.17b, L.sup.3 represents a leaving group
such as halo, p-nitrophenoxy, --OC(O)R.sup.16a, --OC(O)OR.sup.16b,
--OH or imidazole and R.sup.16a, R.sup.16b, R.sup.17a and R.sup.17b
are as hereinbefore defined, for example at between -10.degree. C.
and reflux temperature in the presence of a suitable base (e.g.
triethylamine, pyridine or potassium carbonate), an appropriate
organic solvent (e.g. THF, dichloromethane or acetonitrile) and
(where appropriate) a suitable coupling agent (e.g.
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide); (p) for compounds
of formula I in which R.sup.2 represents H or --OH and R.sup.3
represents H, reduction of a compound of formula I in which R.sup.2
and R.sup.3 together represent .dbd.O, in the presence of a
suitable reducing agent and under appropriate reaction conditions;
for example, for formation of compounds of formula I in which
R.sup.2 represents OH, reduction may be performed under mild
reaction conditions in the presence of e.g. sodium borohydride and
an appropriate organic solvent (e.g. THF); for formation of
compounds of formula I in which R.sup.2 represents OH, wherein the
compound is enantiomerically enriched (or is a single enantiomer)
at the chiral centre to which R.sup.2 is attached, reduction may be
performed enzymatically (for example under conditions known to
those skilled in the art, such as in the presence of horse liver
alcohol dehydrogenase and NADPH) or by hydrogenation in the
presence of a suitable solution-phase (homogeneous) catalyst under
conditions known to those skilled in the art; and for formation of
compounds of formula I in which R.sup.2 represents H, reduction may
be performed either under Wolff-Kischner conditions known to those
skilled in the art or by activating the relevant C.dbd.O group
using an appropriate agent (such as tosylhydrazine) in the presence
of a suitable reducing agent (e.g. sodium borohydride or sodium
cyanoborohydride) and an appropriate organic solvent (e.g. a lower
(e.g. C.sub.1-6) alkyl alcohol); (q) for compounds of formula I in
which R.sup.2 represents halo, substitution of a corresponding
compound of formula I in which R.sup.2 represents --OH, using an
appropriate halogenating agent (e.g. for compounds in which R.sup.2
represents fluoro, reaction with (diethylamino)sulfur trifluoride);
(r) for compounds of formula I in which R.sup.2 and R.sup.3
represent H, A represents -J- and B represents --N(R.sup.20)-Z-
(wherein --N(R.sup.20) is attached to the carbon atom bearing
R.sup.2 and R.sup.3), reaction of a compound of formula XX,
##STR00013##
wherein R.sup.1, R.sup.2, R.sup.3, R.sup.20, R.sup.41 to R.sup.46
and J are as hereinbefore defined, with a compound of formula
XXI,
##STR00014##
wherein R.sup.4, G, Z and L.sup.2 are as hereinbefore defined, for
example at elevated temperature (e.g. 40.degree. C. to reflux) in
the presence of a suitable organic solvent (e.g. acetonitrile); (s)
for compounds of formula I in which A represents Co alkylene and
R.sup.1 and R.sup.3 together represent .dbd.O, reaction of a
compound of formula VII, as hereinbefore defined, with a compound
of formula XXII,
##STR00015##
wherein B, G and R.sup.4 are as hereinbefore defined, for example
at between room and reflux temperature in the presence of a
suitable base (e.g. triethylamine, potassium carbonate or
tetrabutylammonium hydroxide) and an appropriate organic solvent
(e.g. a lower alkyl (e.g. C.sub.1-6) alcohol); (t) for compounds of
formula I in which R.sup.1 represents --C(O)XR.sup.7,
--C(O)N(R.sup.8)R.sup.5d or --S(O).sub.2R.sup.9, reaction of a
compound of formula XXIII,
##STR00016##
wherein R.sup.1a represents --C(O)XR.sup.7, C(O)N(R.sup.8)R.sup.5d
or --S(O).sub.2R.sup.9 and R.sup.5d, R.sup.7, R.sup.8, R.sup.9,
R.sup.41 to R.sup.46 and L.sup.2 are as hereinbefore defined, with
a compound of formula XXIV,
##STR00017##
wherein R.sup.2, R.sup.3, R.sup.4, A, B and G are as hereinbefore
defined, for example at between room and reflux temperature in the
presence of a suitable base (e.g. sodium hydrogencarbonate or
potassium carbonate) and an appropriate organic solvent (e.g.
acetonitrile); (u) for compounds of formula I which are
oxabispidine-nitrogen N-oxide derivatives, oxidation of the
corresponding oxabispidine nitrogen of a corresponding compound of
formula I, in the presence of a suitable oxidising agent (e.g.
mCPBA), for example at 0.degree. C. in the presence of a suitable
organic solvent (e.g. dichloromethane); (v) for compounds of
formula I which are C.sub.1-4 alkyl quaternary ammonium salt
derivatives, in which the alkyl group is attached to a oxabispidine
nitrogen, reaction, at the oxabispidine nitrogen, of a
corresponding compound of formula I with a compound of formula
XXV,
R.sup.25-L.sup.4 XXV
wherein R.sup.25 represents C.sub.1-4 alkyl and L.sup.4 is a
leaving group such as halo, alkanesulfonate or arenesulfonate, for
example at room temperature in the presence of an appropriate
organic solvent (e.g. N,N-dimethylformamide), followed by
purification (using e.g. HPLC) in the presence of a suitable
counter-ion provider (e.g. NH.sub.4OAc); (w) conversion of one
R.sup.4 substituent to another using techniques well known to those
skilled in the art; or (x) introduction of one or more (further)
R.sup.4 substituents to the aromatic ring using techniques well
known to those skilled in the art (e.g. chlorination).
[0037] Compounds of formula II may be prepared by reaction of a
compound of formula XXVI,
##STR00018##
wherein R.sup.41 to R.sup.46 are as hereinbefore defined, with a
compound of formula VIII as hereinbefore defined, for example as
described hereinbefore for the synthesis of compounds of formula I
(process step (d)), or, in the case of compounds of formula II
wherein A represents CH.sub.2 and R.sup.2 represents --OH or
N(H)R.sup.14, wherein R.sup.14 is as hereinbefore defined, with a
compound of formula IX as hereinbefore defined, for example as
described hereinbefore for the synthesis of compounds of formula I
(process step (e)).
[0038] Compounds of formula IV may be prepared by reaction of a
compound of formula II, as hereinbefore defined, with a compound of
formula XXVII,
L.sup.1-C(O)-L.sup.1 XXVII
wherein L.sup.1 is as hereinbefore defined, and in which the two
L.sup.1 groups may be the same or different, for example at between
0.degree. C. and reflux temperature in the presence of a suitable
base (e.g. triethylamine or potassium carbonate) and an appropriate
organic solvent (e.g. toluene or dichloromethane).
[0039] Compounds of formula VII may be prepared by reaction of a
compound of formula XXVI, as hereinbefore defined, with a compound
of formula III, as hereinbefore defined, for example as described
hereinbefore for the synthesis of compounds of formula I (process
step (a)), or, in the case of compounds of formula VII wherein
R.sup.1 represents --C(O)N(H)R.sup.8, with a compound of formula
VI, as hereinbefore defined, for example as described hereinbefore
for the synthesis of compounds of formula I (process step (c)).
[0040] Compounds of formula VII wherein R.sup.1 represents
--C(O)XR.sup.7 or --C(O)N(R.sup.8)R.sup.5d may alternatively be
prepared by reaction of a compound of formula XXVI, as hereinbefore
defined, with a compound of formula XXVII, as hereinbefore defined,
for example as described hereinbefore for the synthesis of
compounds of formula IV, followed by reaction of the resultant
intermediate with a compound of formula V, as hereinbefore defined,
for example as described hereinbefore for the synthesis of
compounds of formula I (process step (b)).
[0041] Compounds of formula VIII may be prepared by standard
techniques. For example, compounds of formula VIII in which: [0042]
(1) B represents -Z-O-- may be prepared by coupling a compound of
formula XI, as hereinbefore defined, to a compound of formula
XXVIII,
[0042] L-Z-C(R.sup.2)(R.sup.3)-A-L.sup.2 XXVIII [0043] wherein
R.sup.2, R.sup.3, A, Z and L.sup.2 are as hereinbefore defined, and
the two L.sup.2 groups may be the same or different; or [0044] (2)
B represents --C(O)N(R.sup.20)-- may be prepared by coupling a
compound of formula XXIX,
[0044] ##STR00019## [0045] wherein G, R.sup.4 and R.sup.20 are as
hereinbefore defined, to a compound of formula XXX,
[0045] L.sup.5-C(O)--C(R.sup.2)(R.sup.3)-A-L.sup.2 XXX [0046]
wherein L.sup.5 represents a suitable leaving group (e.g. --OH or
halo) and R.sup.2, R.sup.3, A and L.sup.2 are as hereinbefore
defined; in both cases, under conditions which are well known to
those skilled in the art.
[0047] Compounds of formula VIII in which A represents
--(CH.sub.2).sub.2--, R.sup.2 and R.sup.3 both represent H, B
represents --CH.sub.2-- and G represents CH may be prepared by
reaction of a compound of formula XXXA,
##STR00020##
wherein R.sup.4 as is hereinbefore defined, but preferably
comprises a single ortho- or para-directing substitutable group,
such as halo, with succinic anhydride under standard Friedel-Crafts
acylation conditions, followed by: [0048] (i) reduction of the
resultant intermediate (which may be a two-step process); [0049]
(ii) conversion of the terminal hydroxy group to an appropriate
L.sup.2 group; and, if necessary, [0050] (iii) conversion of one
R.sup.4 group to another, all of which steps may be carried out
under conditions that are well known to those skilled in the
art.
[0051] Compounds of formula VIII in which A represents C.sub.1-6
alkylene, B represents a direct bond or C.sub.1-4 alkylene, R.sup.2
and R.sup.3 independently represent H or C.sub.1-6 alkyl, provided
that when A represents C.sub.1 alkylene and B represents a single
bond, R.sup.2 and R.sup.3 both represent H, and G represents CH,
may be prepared by coupling a compound of formula XXXB,
##STR00021##
wherein Hal represents fluoro, chloro, bromo or iodo and R.sup.4 is
as hereinbefore defined, to a compound of formula XXXC,
##STR00022##
wherein R.sup.2a and R.sup.3a represent H or C.sub.1-6 alkyl as
appropriate, A.sup.a represents a direct bond or C.sub.1-4
alkylene, B.sup.b represents a direct bond or C.sub.1-4 alkylene,
and Hal, R.sup.2 and R.sup.3 are as hereinbefore defined, or with a
vinyl magnesium halide, for example at between -25.degree. C. and
room temperature in the presence of a suitable zinc(II) salt (e.g.
anhydrous ZnBr.sub.2), an appropriate catalyst (e.g.
Pd(PPh.sub.3).sub.4 or Ni(PPh.sub.3).sub.4) and a reaction-inert
organic solvent (e.g. THF, toluene or diethyl ether), followed by:
[0052] (i) reduction of the resultant intermediate, in the presence
of a suitable borane or borane-Lewis base complex (e.g.
borane-dimethyl sulfide), an appropriate solvent (e.g. diethyl
ether, THF, or a mixture thereof); [0053] (ii) oxidation of the
resulting borane adduct with a suitable oxidising agent (e.g.
sodium perborate); and [0054] (iii) conversion of the resulting OH
group to an L.sup.2 group under conditions known to those skilled
in the art.
[0055] Compounds of formula VIII in which A represents a direct
bond or C.sub.1-6 alkylene, B represents C.sub.2-4 alkylene,
R.sup.2 and R.sup.3 independently represent H or C.sub.1-6 alkyl
and G represents CH may be prepared by coupling a compound of
formula XXXD,
##STR00023##
wherein A.sup.b represents a direct bond or C.sub.1-6 alkylene,
B.sup.c represents a direct bond or C.sub.1-2 alkylene, and
R.sup.2a and R.sup.3a are as hereinbefore defined, or a terminal
alkyne equivalent thereof, with a compound of formula XXXB as
hereinbefore defined, for example under standard metal-catalysed
vinylation conditions, such as Heck conditions (for example in the
presence of suitable palladium catalyst system (e.g. Pd(OAc), and
o-tolylphosphine), for example at between room and reflux
temperature in the presence of a suitable solvent (e.g. THF, DMF,
dimethyl ether, toluene, water, ethanol or mixtures thereof) and
optionally in the presence of an appropriate base (e.g.
triethylamine)), or, where the reaction is carried out using a
terminal alkyne, under coupling conditions that will be known to
those skilled in the art (for example at between room and reflux
temperature in the presence of a suitable solvent (e.g. THF, DMF,
dimethyl ether, toluene, water, ethanol or mixtures thereof), an
appropriate base (e.g. diethylamine) and optionally in the presence
of a suitable catalyst (e.g. a copper salt such as copper(I)
iodide)), followed by: [0056] (i) hydrogenation of the resultant
alkene (or alkyne) intermediate, for example in the presence of a
suitable supported palladium catalyst (e.g. Pd on CaCO.sub.3 or
Pd/C), for example at room temperature in the presence of a
suitable solvent (e.g. a lower alkyl alcohol, such as methanol);
and [0057] (ii) conversion of the OH group to an L.sup.2 group,
under conditions known to those skilled in the art.
[0058] Compounds of formula VIII in which the group
-A-C(R.sup.2)(R.sup.3)--B-- represents --(CH.sub.1).sub.3-11-- may
be prepared by reaction of a corresponding compound of formula VIII
in which the group -A-C(R.sup.2)(R.sup.3)--B-- represents
--(CH.sub.2).sub.1-9-- with diethylmalonate using standard malonic
ester synthesis, followed by: [0059] (i) reduction of the resultant
intermediate; and [0060] (ii) conversion of the terminal hydroxy
group to an appropriate L.sup.2 group, both of which steps may be
carried out under conditions that are well known to those skilled
in the art.
[0061] Compounds of formula VIII in which A represents C.sub.1-6
alkylene, B represents -Z-N(R.sup.20)-- (in which latter case, Z is
attached to the carbon atom bearing R.sup.2 and R.sup.3), G
represents CH and Z and R.sup.20 are as hereinbefore defined, may
be prepared by coupling a compound of formula XXXB as hereinbefore
defined, to a compound of formula XXXE,
##STR00024##
wherein A.sup.c represents C.sub.1-6 alkylene and Z, R.sup.20,
R.sup.2 and R.sup.3 are as hereinbefore defined, for example at
elevated temperature under conditions well known to those skilled
in the art, followed by conversion of the hydroxy group to an
L.sup.2 group under conditions known to those skilled in the art
(for example, where the L.sup.2 group is p-toluenesulfonato, the
conversion may be carried out by reaction between the intermediate
hydroxy compound and p-toluenesulfonyl chloride in the presence of
a suitable base (e.g. triethylamine) and an appropriate solvent
(e.g. dichloromethane), and optionally in the presence of a
suitable catalyst (e.g. DMAP, for example at between 0.1 and 10%
(w/w) (e.g. 1% (w/w)) relative to mass of the intermediate hydroxy
compound).
[0062] Compounds of formula VIII in which A represents a direct
bond or C.sub.1-6 alkylene, B represents C.sub.1-4 alkylene and G
represents N may be prepared by coupling a compound of formula
XXXF
##STR00025##
wherein R.sup.4 is as hereinbefore defined, to a compound of
formula XXXG,
##STR00026##
wherein B.sup.c represents a direct bond or C.sub.1-3 alkylene and
A.sup.c, L.sup.2, L.sup.5, R.sup.2 and R.sup.3 are as hereinbefore
defined, for example by reacting the compound of formula XXXF with
a strong base such as butyl lithium or phenyl lithium (e.g. at
-60.degree. C., in the presence of a polar solvent, such as THF),
followed by addition of the deprotonated intermediate to a compound
of formula XXXG (e.g. at -65.degree. C.) in the presence of a
suitable solvent (such as THF).
[0063] Compounds of formula VIII in which A represents C.sub.2
alkylene and R.sup.2 represents --OR.sup.13, in which R.sup.13
represents C.sub.1-6 alkyl, -E-aryl or -E-Het.sup.6 may
alternatively be prepared by reaction of a compound of formula
XIII, as hereinbefore defined, with a compound of formula XXXI,
##STR00027##
wherein R.sup.3, R.sup.4, R.sup.25, B and G are as hereinbefore
defined, for example at between ambient temperature (e.g.
25.degree. C.) and reflux temperature in the presence of a suitable
base (e.g. potassium carbonate) and an appropriate organic solvent
(e.g. acetonitrile), followed by conversion of the ester
functionality to an L.sup.2 group (in which L.sup.2 is as
hereinbefore defined), under conditions that are well known to
those skilled in the art.
[0064] Compounds of formula IX may be prepared in accordance with
techniques which are known to those skilled in the art. For
example, compounds of formula IX in which: [0065] (1) B represents
--CH.sub.2O-- and Y represents O may be prepared by reaction of a
compound of formula XI, as hereinbefore defined, with a compound of
formula XXXII
[0065] ##STR00028## [0066] wherein R.sup.3 and L.sup.2 are as
hereinbefore defined, for example at elevated temperature (e.g.
between 60.degree. C. and reflux temperature) in the presence of a
suitable base (e.g. potassium carbonate or NaOH) and an appropriate
organic solvent (e.g. acetonitrile or toluene/water), or as
otherwise described in the prior art; [0067] (2) R.sup.3 represents
H, B represents a direct bond, C.sub.1-4 alkylene,
-Z-N(R.sup.20)--, -Z-S(O).sub.n-- or -Z-O-- (in which, in each
case, the group -Z represents C.sub.1-4 alkylene attached to the
carbon atom bearing R.sup.3) and Y represents O may be prepared by
reduction of a compound of formula XXXIIIA or XXXIIIB,
[0067] ##STR00029## [0068] wherein B.sup.a represents
-Z.sup.a-N(R.sup.20), -Z.sup.a-S(O)-- or -Z.sup.a-O-- (in which
groups Z.sup.a represents a direct bond or C.sub.1-3 alkylene
attached to the carbon atom bearing R.sup.3), and B.sup.b, R.sup.4,
R.sup.20, G and n are as hereinbefore defined, for example at
between -15.degree. C. and room temperature in the presence of a
suitable reducing agent (e.g. NaBH.sub.4) and an appropriate
organic solvent (e.g. THF), followed by an internal displacement
reaction in the resultant intermediate, for example at room
temperature in the presence of a suitable base (e.g. potassium
carbonate) and an appropriate organic solvent (e.g. acetonitrile);
[0069] (3) B represents a direct bond, C.sub.1-4 alkylene,
-Z-N(R.sup.20)--, -Z-S(O).sub.2-- or -Z-O-- (in which, in each
case, the group Z represents C.sub.1-4 alkylene attached to the
carbon atom bearing R.sup.3) and Y represents O may be prepared by
oxidation of a compound of formula XXXIVA or XXXIVB,
[0069] ##STR00030## [0070] wherein R.sup.3, R.sup.4, B.sup.a,
B.sup.b and G are as hereinbefore defined, in the presence of a
suitable oxidising agent (e.g. mCPBA), for example by refluxing in
the presence of a suitable organic solvent (e.g. dichloromethane);
or [0071] (4) B represents -Z-O--, in which group Z represents
C.sub.1-4 alkylene attached to the carbon atom bearing R.sup.3, and
Y represents --N(R.sup.14), wherein R.sup.14 represents
--C(O)OR.sup.16b or --S(O).sub.2R.sup.16c, may be prepared by
cyclisation of a compound of formula XXXV,
[0071] ##STR00031## [0072] wherein R.sup.14b represents
--C(O)OR.sup.16b or --S(O).sub.2R.sup.16c, Z.sup.b represents
C.sub.1-4 alkylene attached to the carbon atom bearing R.sup.3 and
R.sup.3, R.sup.4, R.sup.16b, R.sup.16c, G and L are as hereinbefore
defined, for example at between 0.degree. C. and reflux temperature
in the presence of a suitable base (e.g. sodium hydroxide), an
appropriate solvent (e.g. dichloromethane, water, or a mixture
thereof) and, if necessary, a phase transfer catalyst (such as
tetrabutylammonium hydrogensulfate).
[0073] Compounds of formula X may be prepared in a similar fashion
to compounds of formula I (see, for example process steps (a) to
(e)).
[0074] Compounds of formula XIV may be prepared by replacement of
the --OH group of a compound of formula I in which R.sup.2
represents --OH with an L.sup.2 group under conditions that are
known to those skilled in the art.
[0075] Compounds of formula XV in which E represents a direct bond
may be prepared by reaction of a compound of formula I in which
R.sup.2 represents --OH with a compound of formula XXXVI,
R.sup.26S(O).sub.2Cl XXXVI
wherein R.sup.26 represents C.sub.1-4 alkyl or aryl (which two
groups are optionally substituted by one or more substituents
selected from C.sub.1-4 alkyl, halo and nitro), for example at
between -10 and 25.degree. C. in the presence of a suitable is
solvent (e.g. dichloromethane), followed by reaction with a
suitable source of the azide ion (e.g. sodium azide), for example
at between ambient and reflux temperature in the presence of an
appropriate solvent (e.g. N,N-dimethylformamide) and a suitable
base (e.g. sodium hydrogencarbonate).
[0076] Compounds of formula XV may alternatively be prepared by
reaction of a compound of formula VII, as hereinbefore defined,
with a compound of formula XXXVII,
##STR00032##
wherein R.sup.3, R.sup.4, A, B, E, G and L.sup.2 are as
hereinbefore defined, for example under analogous conditions to
those described hereinbefore for the synthesis of compounds of
formula I (process step (d)).
[0077] Compounds of formula XX may be prepared by removing an
optionally substituted benzyloxycarbonyl unit from (i.e.
deprotecting) a corresponding compound of formula I in which B
represents --N(R.sup.20)C(O)OCH.sub.2-- and A represents J, wherein
R.sup.20 and J are as hereinbefore defined, for example under
conditions which are known to those skilled in the art.
[0078] Compounds of formula XXIII may be prepared by reaction of a
compound of formula XXXVIII,
##STR00033##
wherein the wavy bonds indicate optional E-, Z- or mixed E- and
Z-geometry about the double bonds, and R.sup.1a and R.sup.41 to
R.sup.46 are as hereinbefore defined, with water and a suitable
source of the mercury(II) ion (e.g. mercury(II) acetate), for
example at between 0 and 30.degree. C., optionally in the presence
of an appropriate organic solvent (e.g. THF), followed by the
conversion of the two resulting mercurialkyl functions to L.sup.2
groups, wherein L.sup.2 is as hereinbefore defined, under
conditions known to those skilled in the art (for example, in the
case where L.sup.2 represents iodo, reaction with iodine at between
room and reflux temperature in the presence of a suitable solvent
(e.g. chloroform, water or a mixture thereof).
[0079] Compounds of formula XXIII may alternatively be prepared by
reaction of a compound of formula XXXIX,
##STR00034##
wherein R.sup.1a and R.sup.41 to R.sup.46 are as hereinbefore
defined, with a reagent that will convert the two --OH
functionalities to L.sup.2 groups under conditions known to those
skilled in the art. For example, this conversion may be achieved,
in the case of compounds of formula XXIII wherein L.sup.2
represents chloro, bromo or iodo, by reaction of a compound of
formula XXXIX with a suitable halogenating agent (for example:
triphenylphosphine or bis(diphenylphosphino)ethane combined with
the halogen (e.g. bromine or iodine)) in the presence of a suitable
base (e.g. imidazole) and a suitable solvent (e.g. dichloromethane,
ether and/or acetonitrile), for example as described in Synth.
Commun. 1990, 20(10), 1473. Suitable halogenating agents also
include: triphenylphosphine combined with carbon tetrachloride,
carbon tetrabromide, hexachloroethane or hexachloroacetone;
triphenylphosphine dibromide; or triphenylphosphine combined with
diethylazodicarboxylate and methyl iodide. In the case of compounds
of formula XXIII wherein L.sup.2 represents an arenesulfonate or
alkanesulfonate (e.g. p-toluenesulfonate, 2- or
4-nitrobenzenesulfonate, methanesulfonate or
trifluoromethanesulfonate), the conversion may alternatively be
achieved by reaction of a compound of formula XXXIX with an
appropriate arenesulfonyl or alkanesulfonyl derivative (e.g.
p-toluenesulfonyl chloride, 4-nitrobenzenesulfonyl chloride or
trifluoromethanesulfonic anhydride), in the presence of a suitable
base (e.g. triethylamine, pyridine or N,N-diisopropylethylamine)
and an appropriate organic solvent (e.g. dichloromethane or
acetonitrile).
[0080] Compounds of formula XXVI (or an N-protected derivative
thereof may be prepared from a corresponding compound of formula
XL,
##STR00035##
or an N-protected derivative thereof (e.g. where the protecting
group is an R.sup.1a group, wherein R.sup.1a is as hereinbefore
defined), wherein R.sup.41 to R.sup.46 and L.sup.2 are as
hereinbefore defined, with ammonia (or a protected derivative
thereof (e.g. benzylamine)), for example under conditions described
hereinbefore for the synthesis of compounds of formula I (process
step (t)).
[0081] Compounds of formula XXXVII may be prepared in analogous
fashion to compounds of formula XV (i.e. from the corresponding
alcohol).
[0082] Compounds of formula XXXVIII may be prepared by reaction of
a compound of formula XLI,
##STR00036##
wherein the wavy bonds indicate optional E-, Z- or mixed E- and
Z-geometry about the double bonds, and R.sup.41 to R.sup.46 are as
hereinbefore defined, with a compound of formula III in which
R.sup.1 represents --C(O)XR.sup.7, --C(O)N(R.sup.8)R.sup.5d or
--S(O).sub.2R.sup.9, wherein R.sup.5d, R.sup.7, R.sup.8 and R.sup.9
are as hereinbefore defined, for example at between -10 and
25.degree. C. in the presence of a suitable base (e.g. NaOH,
triethylamine, pyridine or potassium carbonate) and an appropriate
solvent (e.g. ether, water, dichloromethane, THF, or mixtures
thereof).
[0083] Compounds of formula XXXIX may be prepared by reaction of a
compound of formula XLII,
##STR00037##
wherein the wavy bonds indicate optional R-, S- or mixed R- and
S-stereochemistry at the asymmetric carbon atoms, and R.sup.1a and
R.sup.41 to R.sup.46 are as hereinbefore defined, with water, for
example at between room and reflux temperature in the presence of a
suitable catalyst (e.g. a protic acid such as sulfuric,
methanesulfonic or trifluoroacetic acid, an acidic ion-exchange
resin such as Amberlyst.RTM. 15 or Nafion.RTM., a Lewis acid such
as ZnSO.sub.4 or Yb(III) trifluoromethanesulfonate or a base such
as sodium hydroxide or tetrabutylammonium hydroxide), an
appropriate solvent (e.g. THF, water, 1,4-dioxane or
1-methyl-2-pyrrolidinone, or mixtures thereof (e.g. THF/water)),
and optionally (when a basic catalyst is used) in the presence of a
suitable phase transfer catalyst (e.g. Triton.RTM. B).
[0084] The reaction may be advantageously be performed using
compounds of formula XLII having enantiomeric (or diastereomeric)
enrichment at the chiral centres identified above. The use of such
enantiomerically- (or diastereomerically-) enriched compounds of
formula XLII in the formation of compounds of formula XXXIX may
have the advantage that a greater proportion of the product diol is
obtained as the cis-isomer (i.e. the conformation of compounds of
formula XXXIX depicted above). Those skilled in the art will
appreciate that such an increased proportion of cis-isomer may be
retained in the conversion of compounds of formula XXXIX to
compounds of formula XXIII, and thus may eventually lead to a
higher yield of compounds of formula I (via process step (t)).
[0085] The reaction may also be advantageously performed using
compounds of formula XLII wherein R.sup.1a represents
--S(O).sub.2R.sup.9 (e.g. wherein R.sup.9 represents optionally
substituted phenyl, such as 2- or 4-fluorophenyl, 2- or
4-chlorophenyl, 4-bromophenyl, 4-methylphenyl, 4-methoxyphenyl, 2-
or 4-nitrophenyl, 2,4,6-trimethylphenyl). The use of such
ring-substituted benzenesulfonyl derivatives may have the advantage
that purification of the resulting compound of formula XXXIX may be
made more straightforward (e.g. requiring only a simple
recrystallisation step) and/or that, in a compound of formula I
(synthesised via compounds of formulae XXXIX and XXIII), removal of
the --S(O).sub.2R.sup.9 group (allowing its replacement with
another R.sup.1 group) may be made more straightforward (e.g.
enabling the use of milder reaction conditions).
[0086] Compounds of formula XL may be prepared in an analogous
fashion to compounds of formula XXIII, as hereinbefore defined
(i.e. from e.g. the corresponding diallylamine).
[0087] Compounds of formula XLII in which the substituent R.sup.42
has the same identity as R.sup.41, R.sup.44 has the same identity
as R.sup.43 and R.sup.46 has the same identity as R.sup.45 may be
prepared by reaction of two or more equivalents of a compound of
formula XLIII,
##STR00038##
wherein the wavy bond indicates optional R-, S- or mixed R- and
S-stereochemistry at the asymmetric carbon atom, and R.sup.41,
R.sup.43, R.sup.45 and L.sup.2 are as hereinbefore defined, with
one equivalent of a compound of formula XLIV,
R.sup.1aNH.sub.2 XLIV
wherein R.sup.1a is as hereinbefore defined, for example at between
room and reflux temperature in the presence of a suitable base
(e.g. an alkali metal carbonate such as cesium carbonate, sodium
hydroxide, sodium hydride or lithium diisopropylamide), an
appropriate solvent (e.g. acetonitrile, N,N-dimethylformamide, THF,
toluene, water or mixtures thereof), and optionally in the presence
of a phase transfer catalyst (e.g. tricaprylyl-methylammonium
chloride). Preferred bases include sodium hydroxide and preferred
solvents include water. The reaction is advantageously performed
with compounds of formula XLIV wherein R.sup.1a represents
--S(O).sub.2R.sup.9 (e.g. wherein R.sup.9 represents optionally
substituted phenyl, such as 2- or 4-fluorophenyl, 2- or
4-chlorophenyl, 4-bromophenyl, 4-methylphenyl, 4-methoxyphenyl, 2-
or 4-nitrophenyl, 2,4,6-trimethylphenyl). The use of such
ring-substituted benzenesulfonyl derivatives may have the advantage
that purification of the resulting compound of formula XLII may be
made more straightforward (e.g. requiring only a simple
recrystallisation step).
[0088] Compounds of formulae XLIII and XLIV may also be reacted
together in this way in the presence of water in order to give a
direct, "one-pot" process providing a compound of formula XXXIX.
Such "one-pot" reactions may be carried out, for example, by using
a biphasic reaction mixture comprising a solution of XLIII and XLIV
in an organic solvent (e.g. toluene), and an aqueous solution of a
base (e.g. sodium hydroxide). Alternatively, the aqueous solution
of base may, after the formation of the intermediate compound of
formula XLII is complete, be exchanged for an aqueous solution of
an acid (e.g. either a protic or a Lewis acid). Any of the reaction
steps in such biphasic mixtures may be carried out in the presence
of a suitable phase transfer catalyst.
[0089] Compounds of formula XLII having enantiomeric (or
diastereomeric) enrichment at the chiral centres identified above
may be prepared by reaction of a compound of formula XLIV, as
hereinbefore defined, with a compound of formula XLIII, as
hereinbefore defined, having enantiomeric enrichment at the carbon
atom to which R.sup.43 is attached. Those skilled in the art will
realise that this will lead to more of the isomer(s) required for
further elaboration to compounds of formula I.
[0090] Compounds of formula XLII may alternatively be prepared by
reaction of a compound of formula XXXVIII, as hereinbefore defined,
with a suitable oxidising agent. Suitable conditions for this
oxidation include, for example, reaction at between -25.degree. C.
and reflux temperature with a suitable peroxide or peracid (e.g.
hydrogen peroxide, tert-butyl hydroperoxide or mCPBA), optionally
in the presence of an appropriate solvent (e.g. dichloromethane,
t-butanol, nitromethane, toluene, water, or mixtures thereof), a
suitable catalyst (for example a protic acid, a Lewis acid, or a
metal complex capable of forming a peroxide adduct, such as
methyltrioxorhenium(VII) or a combination of sodium tungstate and
(aminomethyl)phosphonic acid), and/or further appropriate additives
(for example: in the case of oxidations carried out with
methyltrioxorhenium(VII) and hydrogen peroxide, a basic additive
such as pyridine or pyrazole; and in the case of oxidations with
sodium tungstate and hydrogen peroxide, a phase transfer catalyst
such as methyltri-n-octylammonium hydrogensulfate). Particular
embodiments of this oxidation are described in patent applications
EP A1 0 380 085 and WO 98/33786 A1, the disclosures in which
documents are hereby incorporated by reference. When the oxidation
is carried out in the presence of both a catalyst and water, one
embodiment of the reaction involves a "one-pot" conversion of the
compound of formula XXXVIII to a compound of formula XXXIX, as
hereinbefore defined. This reaction proceeds via the catalysed
hydrolysis of the intermediate compound of formula XLII.
[0091] Compounds of formula XLII in which the two epoxide chains
are not identical (e.g. where R.sup.41 and R.sup.42 are not
identical) may be prepared by reaction of a compound of formula
XLIII, as hereinbefore defined, with a compound of formula XLV,
##STR00039##
wherein the wavy bond indicates optional R-, S- or mixed R- and
S-stereochemistry at the asymmetric carbon atom, and R.sup.1a,
R.sup.42, R.sup.44, and R.sup.46 are as hereinbefore defined, for
example under conditions described hereinbefore for the synthesis
of symmetrical compounds of formula XLII.
[0092] Compounds of formula XLV may be prepared by reaction of one
or more equivalents of a compound of formula XLIV, as hereinbefore
defined, with one equivalent of a compound of formula XLIII, for
example under conditions as described hereinbefore for reaction
between these two compounds.
[0093] Compounds of formula XLV may alternatively be prepared by
oxidation of a corresponding compound of formula XLVI,
##STR00040##
wherein the wavy bond indicates optional E-, Z- or mixed E- and
Z-geometry about the double bond, and R.sup.1a, R.sup.42, R.sup.44
and R.sup.46 are as hereinbefore defined, for example under
conditions as hereinbefore described for the synthesis of compounds
of formula XLII.
[0094] Compounds of formula I may also be prepared, advantageously,
by dehydrative cyclisation of compound of formula XLVII,
##STR00041##
wherein A, B, G, R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.41 to
R.sup.46 are as hereinbefore defined, for example in the presence
of a suitable dehydrating agent (such as: a strong acid (e.g.
sulfuric acid (e.g. concentrated sulfuric acid), methanesulfonic
acid (e.g. anhydrous methanesulfonic acid) and the like; an acid
anhydride such as acetic anhydride or trifluoromethane-sulfonic
anhydride; P.sub.2O.sub.5 in methanesulfonic acid; a
phosphorous-based halogenating agent such as P(O)Cl.sub.3,
PCl.sub.3 or PCl.sub.5; and thionyl chloride).
[0095] This cyclisation process may be carried out in the presence
of a suitable organic solvent system, which solvent system should
not significantly react chemically with, or significantly give rise
to stereochemical changes in, the reactants or product once formed,
or significantly give rise to other side reactions. Preferred
solvent systems include aromatic hydrocarbons (e.g. toluene or
xylene).
[0096] This cyclisation process may be carried out at elevated
temperature (e.g. up to the reflux temperature of the relevant
solvent system, or higher if a pressurised system is employed).
Clearly, appropriate reaction times and reaction temperatures
depend upon the solvent system that is employed, as well as the
reactants that are used and the compound that is to be formed, but
these may be determined routinely by the skilled person.
[0097] Compounds of formula XLVII may advantageously be prepared by
reaction of a compound of formula XLVIII,
##STR00042##
wherein the wavy bonds indicate optional R-, S- or mixed R- and
S-stereochemistry at the asymmetric carbon atoms, and R.sup.1 and
R.sup.41 to R.sup.46 are as hereinbefore defined, with a compound
of formula XXIV as hereinbefore defined. This reaction may be
carried out at between room temperature and the reflux temperature
of any solvent that is employed. Suitable solvent systems that may
be employed include organic solvent systems, which system should
not significantly react chemically with, or significantly give rise
to stereochemical changes in, the reactants or product once formed,
or significantly give rise to other side reactions. Preferred
solvent systems include lower alkyl alcohols (particularly primary
alcohols (e.g. ethanol)) optionally in the presence of water, IMS,
aromatic hydrocarbons (e.g. toluene) or mixtures thereof.
[0098] Compounds of formula XXIV may be prepared as described
herein. Compounds of formula XLVIII may be prepared according to or
by analogy with the procedures described herein in relation to the
preparation of compounds of formula XLII.
[0099] The formation of compounds of formula XLVII may be also be
performed using compounds of formula XLVI having enantiomeric (or
diastereomeric) enrichment at the chiral centres identified
hereinbefore. The use of such enantiomerically- (or
diastereomerically-) enriched compounds of formula XLVIII in the
formation of compounds of formula XLVII may have the advantage that
a greater proportion of the product diol is obtained in a form
(e.g. the trans-form) which facilitates the subsequent cyclisation,
leading to a higher yield of compounds of formula I.
[0100] The formation of compounds of formula XLVII is preferably
carried out using compounds of formula XLVIII in which R.sup.1
represents R.sup.1a, wherein R.sup.1a is as hereinbefore defined.
The formation of compounds of formula XLVII is more preferably
carried out using compounds of formula XLVIII in which R.sup.1
represents --S(O).sub.2R.sup.9 (e.g. wherein R.sup.9 represents
optionally substituted phenyl, such as 2- or 4-fluorophenyl, 2- or
4-chlorophenyl, 4-bromophenyl, 4-methylphenyl, 4-methoxyphenyl, 2-
or 4-nitrophenyl, 2,4,6-trimethylphenyl and, especially,
unsubstituted phenyl).
[0101] Preferred compounds of formula XXIV include those in
which:
G represents CH; A represents a direct bond; B represents a direct
bond; R.sup.2 represents H or C.sub.1-6 alkyl; R.sup.3 represents H
or C.sub.1-6 alkyl; R.sup.4 is absent or represents one to three
halo, methyl, methoxy or nitro groups, especially 2- or 4-fluoro,
2- or 4-chloro, 4-bromo, 4-methyl, 2,4,6-trimethyl, 4-methoxy, or
2- or 4-nitro.
[0102] We have found, surprisingly, that, when compounds of formula
I are formed using this process (i.e. via compounds of formula
XLVII), the employment of derivatives of formula XLVIII in which
R.sup.1 represents R.sup.1a (e.g. wherein R.sup.1a represents
optionally-substituted benzenesulfonyl, such as described above),
and benzylamine-type derivatives of formula XXIV (such as those
described above), may have the advantage that, in the resultant
compound of formula I, the presence of the R.sup.1a (e.g.
--S(O).sub.2R.sup.9) group and/or the benzylamine-type group allows
for direct and facile replacement of that/those group(s) with other
R.sup.1 groups, and/or
##STR00043##
fragments, as appropriate, for example by employing reactions that
are akin to "deprotection" reactions (see below), and subsequently
performing coupling reactions (see, for example process steps (a),
(c), (d) and (e)). We have found, if that benzenesulfonyl
derivatives of formula XLVIII, and benzylamine-type derivatives of
formula XXIV are employed, subsequent replacement steps may be made
more straightforward (e.g. enabling the use of milder reaction
conditions).
[0103] In this respect, certain compounds of the invention may
further be employed as intermediates, useful in the manufacture of
other compounds of the invention. Such compounds include, but are
not limited to compounds of formula I in which:
R.sup.1 represents --S(O).sub.2R.sup.9, wherein R.sup.9 represents
optionally substituted phenyl, such as 2- or 4-fluorophenyl, 2- or
4-chlorophenyl. 4-bromophenyl, 4-methylphenyl, 4-methoxyphenyl, 2-
or 4-nitrophenyl, 2,4,6-trimethylphenyl and, especially,
unsubstituted phenyl; R.sup.41 to R.sup.46 all represent H; G
represents CH; A represents a direct-bond; B represents a direct
bond; R.sup.2 represents H or C.sub.1-6 alkyl; R.sup.3 represents H
or C.sub.1-6 alkyl; R.sup.4 is absent or represents one to three
halo, methyl, methoxy or nitro groups, especially 2- or 4-fluoro,
2- or 4-chloro, 4-bromo, 4-methyl, 2,4,6-trimethyl, 4-methoxy, or
2- or 4-nitro.
[0104] Compounds of formula I that may, in particular, be employed
as intermediates include, but are not limited to those in
which:
R.sup.2 and R.sup.3 both represent H; R.sup.4 is absent; and/or
R.sup.9 represents unsubstituted phenyl.
[0105] Further, compounds of formula I in which:
R.sup.41 to R.sup.46 all represent H; R.sup.1 represents straight-
or branched-chain C.sub.1-4 alkyl (e.g. C.sub.1-3 alkyl, such as
methyl) terminated by C(O)R.sup.5a or --N(H)C(O)OR.sup.10b;
R.sup.5a and R.sup.10b independently represent straight- or
branched-chain C.sub.2-6 alkyl (e.g. C.sub.3-5 alkyl, such butyl
(e.g. t-butyl)); R.sup.2 represents H or OH; R.sup.3 represents H;
A represents C.sub.1 alkylene or linear C.sub.2 alkylene; B
represents -Z-, -Z-N(H)-- or -Z-O-- (in which latter two groups, Z
is attached to the carbon atom bearing R.sup.2 and R.sup.3, and
represents C.sub.1 alkylene or linear C.sub.2 alkylene); G
represents CH; and R.sup.4 is a single cyano group in the
para-position relative to B, may be prepared by a process which
comprises the steps of: [0106] (i) removal of the --SO.sub.2R.sup.9
group from a compound of formula I in which R.sup.1 represents
--S(O).sub.2R.sup.9, wherein R.sup.9 represents optionally
substituted phenyl, R.sup.41 to R.sup.46 all represent H, G
represents CH, A and B both represent direct bonds, R.sup.2 and
R.sup.3 independently represent H or C.sub.1-6 alkyl and R.sup.4 is
absent or represents one to three halo, methyl, methoxy or nitro
groups, especially 2- or 4-fluoro, 2- or 4-chloro, 4-bromo,
4-methyl, 2,4,6-trimethyl, 4-methoxy, or 2- or 4-nitro, to provide
a compound of formula II as hereinbefore defined, in which R.sup.41
to R.sup.1 all represent H, G represents CH, A and B both represent
direct bonds, R.sup.2 and R.sup.3 independently represent H or
C.sub.1-6 alkyl and R.sup.4 is absent or represents one to three
halo, methyl, methoxy or nitro groups, especially 2- or 4-fluoro,
2- or 4-chloro, 4-bromo, 4-methyl, 2,4,6-trimethyl, 4-methoxy, or
2- or 4-nitro, for example using standard deprotection conditions
(e.g. in the presence of a standard deprotecting agent (such a
hydrohalic acid (e.g. HBr, especially concentrated aqueous HBr) or
a reducing agent such as LiAlH.sub.4), at or above room temperature
(e.g. at reflux) with or without the presence of a solvent; [0107]
(ii) reaction of the resultant compound of formula II with a
compound of formula III, as hereinbefore defined, in which R.sup.1
represents straight- or branched-chain C.sub.1-4 alkyl (e.g.
C.sub.1-3 alkyl, such as methyl) terminated by C(O)R.sup.5a or
--N(H)C(O)OR.sup.10b, in which R.sup.5a and R.sup.10b independently
represent straight- or branched-chain C.sub.2-6 alkyl (e.g.
C.sub.3-5 alkyl, such butyl (e.g. t-butyl)), to form a compound of
formula I in which R.sup.1 represents straight- or branched-chain
C.sub.1-4 alkyl (e.g. C.sub.1-3 alkyl, such as methyl) terminated
by C(O)R.sup.5a or --N(H)C(O)OR.sup.10b, R.sup.5a and R.sup.10b
independently represent straight- or branched-chain C.sub.2-6 alkyl
(e.g. C.sub.3-5 alkyl, such butyl (e.g. t-butyl)), R.sup.41 to
R.sup.46 all represent H, G represents CH, A and B both represent a
direct bond, R.sup.2 and R.sup.3 independently represent H or
C.sub.1-6 alkyl and R.sup.4 is absent or represents one to three
halo, methyl, methoxy or nitro groups, especially 2- or 4-fluoro,
2- or 4-chloro, 4-bromo, 4-methyl, 2,4,6-trimethyl, 4-methoxy, or
2- or 4-nitro, for example under conditions described hereinbefore
(see e.g. process (a)), e.g. in the presence of a suitable solvent
(e.g. water, a lower alkyl alcohol, acetonitrile, or mixtures
thereof) and an appropriate base (e.g. sodium bicarbonate or
potassium carbonate); [0108] (iii) removal of the
[0108] ##STR00044## [0109] fragment from the resultant compound of
formula I to provide a compound of formula VII in which R.sup.1
represents straight- or branched-chain C.sub.1-4 alkyl (e.g.
C.sub.1-3 alkyl, such as methyl) terminated by C(O)R.sup.5a or
--N(H)C(O)OR.sup.10b, R.sup.5a and R.sup.10b independently
represent straight- or branched-chain C.sub.2-6 alkyl (e.g.
C.sub.3-5 alkyl, such butyl (e.g. t-butyl)), and R.sup.41 to
R.sup.46 all represent H, for example under appropriate
deprotection conditions, such as hydrogenation in the presence of a
supported palladium catalyst (e.g. Pd/C), for example at room
temperature in the presence of a suitable solvent (e.g. a lower
alkyl alcohol, such as ethanol)); and [0110] (iv) reaction of the
resultant compound of formula VII with a compound of formula VIII
as hereinbefore defined, in which R.sup.1 represents H or OH,
R.sup.3 represents H, A represents C.sub.1 alkylene or linear
C.sub.2 alkylene, B represents -Z-, -Z-N(H)-- or -Z-O-- (in which
latter two groups, Z is attached to the carbon atom bearing R.sup.2
and R.sup.3, and represents C.sub.1 alkylene or linear C.sub.2
alkylene), G represents CH and R.sup.4 is a single cyano group in
the para-position relative to B, and L.sup.2 represents, for
example, arenesulfonate (e.g. toluenesulfonate), for example under
conditions described hereinbefore (see e.g. process (d)), such as
at between room and reflux temperature, in the presence of a
suitable base (e.g. potassium carbonate) and an appropriate organic
solvent (e.g. a lower alkyl alcohol, such as ethanol).
[0111] The skilled person will appreciate that, if desired, the
above steps may be performed in a different order to those that
stated above, to provide the relevant compounds of formula I. For
example, steps (iii) and (iv) may be carried out prior to steps (i)
and (ii). Alternatively, steps (i) and (iii) (in either order) may
be completed before steps (ii) and (iv) (in either order) are
carried out. However, we prefer that the steps are performed in the
above-stated order.
[0112] The process of making the compounds of formula I from
compounds of formulae XLVIII and XXIV (i.e. via compounds of
formula XLVII) may have the advantage that oxabispidine ring
systems may be formed using fewer steps than methods described in
the prior art, and, particularly, avoids the use of
mercury-containing compounds (thereby eliminating the production of
toxic, mercury-containing waste). This process offers a convenient
synthetic route to key oxabispidine compounds, and allows
differential protection at the nitrogen atoms.
[0113] Further, this process may have the advantage that compounds
comprising the oxabispidine ring may be prepared in less time, more
conveniently, and/or at a lower cost, than when prepared in
processes described in the prior art.
[0114] Compounds of formulae III, V, VI, XI, XII, XIII, XVI, XVII,
XVIII, XIX, XXI, XXII, XXIV, XXV, XXVII, XXVIII, XXIX, XXX, XXXA,
XXXB, XXXC, XXXD, XXXE, XXXF, XXXG, XXXI, XXXII, XXXIIIA, XXXIIIB,
XXXIVA, XXXIVB, XXXV, XXXVI, XLI, XLIII, XLIV and XLVI and
derivatives thereof, are either commercially available, are known
in the literature, or may be obtained either by analogy with the
processes described herein, or by conventional synthetic
procedures, in accordance with standard techniques, from readily
available starting materials using appropriate reagents and
reaction conditions.
[0115] Substituents on the aryl (e.g. phenyl), and (if appropriate)
heterocyclic, group(s) in compounds defined herein may be converted
to other claimed substituents using techniques well known to those
skilled in the art. For example, hydroxy may be converted to
alkoxy, phenyl may be halogenated to give halophenyl, nitro may be
reduced to give amino, halo may be displaced by cyano, etc.
[0116] The skilled person will also appreciate that various
standard substituent or functional group interconversions and
transformations within certain compounds of formula I will provide
other compounds of formulae I. For example, carbonyl may be reduced
to hydroxy or alkylene, and hydroxy may be converted to halo.
[0117] The compounds of the invention may be isolated from their
reaction mixtures using conventional techniques.
[0118] It will be appreciated by those skilled in the art that, in
the process described above, the functional groups of intermediate
compounds may be, or may need to be, protected by protecting
groups.
[0119] Functional groups which it is desirable to protect include
hydroxy, amino and carboxylic acid. Suitable protecting groups for
hydroxy include trialkylsilyl and diarylalkylsilyl groups (e.g.
tert-butyldimethylsilyl, tert-butyldiphenylsilyl or
trimethylsilyl), tetrahydropyranyl and alkylcarbonyl groups (e.g.
methyl- and ethylcarbonyl groups). Suitable protecting groups for
amino include benzyl, sulfonamido (e.g. benzenesulfonamido),
tert-butyloxycarbonyl, 9-fluorenyl-methoxycarbonyl or
benzyloxycarbonyl. Suitable protecting groups for amidino and
guanidino include benzyloxycarbonyl. Suitable protecting groups for
carboxylic acid include C.sub.1-6 alkyl or benzyl esters.
[0120] The protection and deprotection of functional groups may
take place before or after any of the reaction steps described
hereinbefore.
[0121] Protecting groups may be removed in accordance with
techniques which are well known to those skilled in the art and as
described hereinafter. For example, we have found that removal of
an --SO.sub.2R.sup.9 group from an oxabispidine ring may take place
conveniently by employment of an appropriate strong acid, such as a
hydrohalic acid (especially HBr) e.g. as described
hereinbefore.
[0122] The use of protecting groups is fully described in
"Protective Groups in Organic Chemistry", edited by J. W. F.
McOmie, Plenum Press (1973), and "Protective Groups in Organic
Synthesis", 3.sup.rd edition, T. W. Greene & P. G. M. Wutz,
Wiley-Interscience (1999).
[0123] Persons skilled in the art will appreciate that, in order to
obtain compounds of the invention in an alternative, and, on some
occasions, more convenient, manner, the individual process steps
mentioned herein may be performed in a different order, and/or the
individual reactions may be performed at a different stage in the
overall route (i.e. substituents may be added to and/or chemical
transformations performed upon, different intermediates to those
associated hereinbefore with a particular reaction). This will
depend inter alia on factors such as the nature of other functional
groups present in a particular substrate, the availability of key
intermediates and the protecting group strategy (if any) to be
adopted. Clearly, the type of chemistry involved will influence the
choice of reagent that is used in the said synthetic steps, the
need, and type, of protecting groups that are employed, and the
sequence for accomplishing the synthesis.
[0124] It will also be appreciated by those skilled in the art
that, although certain protected derivatives of compounds of
formula I, which may be made prior to a final deprotection stage,
may not possess pharmacological activity as such, they may be
administered parenterally or orally and thereafter metabolised in
the body to form compounds of the invention which are
pharmacologically active. Such derivatives may therefore be
described as "prodrugs". Moreover, certain compounds of formula I
may act as prodrugs of other compounds of formula I.
[0125] All prodrugs of compounds of formula I are included within
the scope of the invention.
[0126] Some of the intermediates referred to hereinbefore are
novel. According to a further aspect of the invention there is thus
provided: (a) a compound of is formula II, as hereinbefore defined,
or a protected derivative thereof, optionally in the form of a salt
and/or a solvate; (b) a compound of formula IV, as hereinbefore
defined, or a protected derivative thereof; (c) a compound of
formula VII, as hereinbefore defined, or a protected derivative
thereof (provided that R.sup.1 does not represent
--S(O).sub.2R.sup.9, wherein R.sup.9 represents unsubstituted
phenyl). Preferred compounds of formula VII include those in which
R.sup.1 does not represent C(O)OR.sup.7, in which R.sup.7 is
tert-butyl; (d) a compound of formula X, as hereinbefore defined,
or a protected derivative thereof; (e) a compound of formula XIV,
as hereinbefore defined, or a protected derivative thereof; (f) a
compound of formula XV, as hereinbefore defined, or a protected
derivative thereof; (g) a compound of formula XX, as hereinbefore
defined, or a protected derivative thereof; (h) a compound of
formula XXIII, as hereinbefore defined, or a protected derivative
thereof, provided that L.sup.2 does not represent iodo; (i) a
compound of formula XXXIX, or a protected derivative thereof; and
(j) a compound of formula XLII, or a protected derivative
thereof.
[0127] Preferred compounds of formula II include those in
which:
R.sup.41 to R.sup.46 all represent H; G represents CH; A represents
a direct bond; B represents a direct bond; R.sup.2 represents H or
Clot alkyl; R.sup.3 represents H or C.sub.1-6 alkyl; and/or R.sup.4
is absent or represents one to three halo, methyl, methoxy or nitro
groups.
[0128] Particularly preferred compounds of formula II include those
in which: R.sup.2 and R.sup.3 both represent H, and R.sup.4 is
absent, optionally in the form of a sulfate, hemisulfate or,
especially, a hydrochloride (such as a dihydrochloride) salt, which
salt is optionally a hydrate (e.g. a hemihydrate).
[0129] Preferred compounds of formula VII include those which are
not: [0130] tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate.
[0131] Preferred compounds of formula XLVII include those in which
the group R.sup.1 and the group
##STR00045##
are different, and as such include those compounds of formula XLVII
in which: R.sup.1 represents --C(O)XR.sup.7,
--C(O)N(R.sup.8)R.sup.5d or --S(O).sub.2R.sup.9 (wherein X,
R.sup.5d, R.sup.7, R.sup.8 and R.sup.9 are as hereinbefore
defined); R.sup.2 and R.sup.3 do not together represent .dbd.O when
A represents a direct bond.
[0132] Particularly preferred compounds of formula XLVII include
those in which
R.sup.1 represents --S(O).sub.2R.sup.9, wherein R.sup.9 represents
aryl (such as phenyl, particularly unsubstituted phenyl); G
represents CH; A represents a direct bond; B represents a direct
bond; R.sup.2 represents H or C.sub.1-6 alkyl; R.sup.3 represents H
or C.sub.1-6 alkyl;
[0133] R.sup.4 is absent or represents one to three halo, methyl,
methoxy or nitro groups, especially 2- or 4-fluoro, 2- or 4-chloro,
4-bromo, 4-methyl, 2,4,6-trimethyl, 4-methoxy, or 2- or
4-nitro.
Medical and Pharmaceutical Use
[0134] Compounds of the invention are useful because they possess
pharmacological activity. They are therefore indicated as
pharmaceuticals.
[0135] Thus, according to a further aspect of the invention there
is provided the compounds of the invention for use as
pharmaceuticals.
[0136] In particular, the compounds of the invention exhibit
myocardial electrophysiological activity, for example as
demonstrated in the test described below.
[0137] The compounds of the invention are thus expected to be
useful in both the prophylaxis and the treatment of arrhythmias,
and in particular atrial and ventricular arrhythmias.
[0138] The compounds of the invention are thus indicated in the
treatment or prophylaxis of cardiac diseases, or in indications
related to cardiac diseases, in which arrhythmias are believed to
play a major role, including ischaemic heart disease, sudden heart
attack, myocardial infarction, heart failure, cardiac surgery and
thromboembolic events.
[0139] In the treatment of arrhythmias, compounds of the invention
have been found to selectively delay cardiac repolarization, thus
prolonging the QT interval, and, in particular, to exhibit class
III activity. Although compounds of the invention have been found
to exhibit class III activity in particular, in the treatment of
arrhythmias, their mode(s) of activity is/are not necessarily
restricted to this class.
[0140] According to a further aspect of the invention, there is
provided a method of treatment of an arrhythmia which method
comprises administration of a therapeutically effective amount of a
compound of the invention to a person suffering from, or
susceptible to, such a condition.
Pharmaceutical Preparations
[0141] The compounds of the invention will normally be administered
orally, subcutaneously, intravenously, intraarterially,
transdermally, intranasally, by inhalation, or by any other
parenteral route, in the form of pharmaceutical preparations
comprising the active ingredient either as a free base or a
non-toxic organic or inorganic acid addition salt, in a
pharmaceutically acceptable dosage form. Depending upon the
disorder and patient to be treated, as well as the route of
administration, the compositions may be administered at varying
doses.
[0142] The compounds of the invention may also be combined with any
other drugs useful in the treatment of arrhythmias and/or other
cardiovascular disorders.
[0143] According to a further aspect of the invention there is thus
provided a pharmaceutical formulation including a compound of the
invention in admixture with a pharmaceutically acceptable adjuvant,
diluent or carrier.
[0144] Suitable daily doses of the compounds of the invention in
therapeutic treatment of humans are about 0.005 to 25.0 mg/kg body
weight at oral administration and about 0.005 to 10.0 mg/kg body
weight at parenteral administration. Preferable ranges of daily
doses of the compounds of the invention in therapeutic treatment of
humans are about 0.005 to 10.0 mg/kg body weight at oral
administration and about 0.005 to 5.0 mg/kg body weight at
parenteral administration.
[0145] The compounds of the invention have the advantage that they
are effective against cardiac arrhythmias.
[0146] Compounds of the invention may also have the advantage that
they may be more efficacious than, be less toxic than, have a
broader range of activity (including exhibiting any combination of
class I, class II, class III and/or class IV activity (especially
class I and/or class IV activity in addition to class III
activity)) than, be more potent than, be longer acting than,
produce fewer side effects (including a lower incidence of
proarrhythmias such as torsades de pointes) than, be more easily
absorbed than, or that they may have other useful pharmacological
properties over, compounds known in the prior art.
Biological Tests
Test A
Primary Electrophysiological Effects In Anaesthetised Guinea
Pigs
[0147] Guinea pigs weighing between 660 and 1100 g were used. The
animals were housed for at least one week before the experiment and
had free access to food and tap water during that period.
[0148] Anaesthesia was induced by an intraperitoneal injection of
pentobarbital (40 to 50 mg/kg) and catheters were introduced into
one carotid artery (for blood pressure recording and blood
sampling) and into one jugular vein (for is drug infusions). Needle
electrodes were placed on the limbs for recording of ECGs (lead
II). A thermistor was placed in the rectum and the animal was
placed on a heating pad, set to a rectal temperature of between
37.5 and 38.5.degree. C.
[0149] A tracheotomy was performed and the animal was artificially
ventilated with room air by use of a small animal ventilator, set
to keep blood gases within the normal range for the species. In
order to reduce autonomic influences both vagi were cut in the
neck, and 0.5 mg/kg of propranolol was given intravenously, 15
minutes before the start of the experiment.
[0150] The left ventricular epicardium was exposed by a left-sided
thoracotomy, and a custom-designed suction electrode for recording
of the monophasic action potential (MAP) was applied to the left
ventricular free wall. The electrode was kept in position as long
as an acceptable signal could be recorded, otherwise it was moved
to a new position. A bipolar electrode for pacing was clipped to
the left atrium. Pacing (2 ms duration, twice the diastolic
threshold) was performed with a custom-made constant current
stimulator. The heart was paced at a frequency just above the
normal sinus rate during 1 minute every fifth minute throughout the
study.
[0151] The blood pressure, the MAP signal and the lead II ECG were
recorded on a Mingograph ink-jet recorder (Siemens-Elema, Sweden).
All signals were collected (sampling frequency 1000 Hz) on a PC
during the last 10 seconds of each pacing sequence and the last 10
seconds of the following minute of sinus rhythm. The signals were
processed using a custom-made program developed for acquisition and
analysis of physiological signals measured in experimental animals
(see Axenborg and Hirsch, Comput. Methods Programs Biomed. 41, 55
(1993)).
[0152] The test procedure consisted of taking two basal control
recordings, 5 minutes apart, during both pacing and sinus rhythm.
After the second control recording, the first dose of the test
substance was infused in a volume of 0.2 mL into the jugular vein
catheter for 30 seconds. Three minutes later, pacing was started
and a new recording was made. Five minutes after the previous dose,
the next dose of test substance was administered. Six to ten
consecutive doses were given during each experiment.
Data Analysis
[0153] Of the numerous variables measured in this analysis, three
were selected as the most important for comparison and selection of
active compounds. The three variables selected were the MAP
duration at 75 percent repolarization during pacing, the
atrio-ventricular (AV) conduction time (defined as the interval
between the atrial pace pulse and the start of the ventricular MAP)
during pacing, and the heart rate (defined as the RR interval
during sinus rhythm). Systolic and diastolic blood pressure were
measured in order to judge the haemodynamic status of the
anaesthetised animal. Further, the ECG was checked for arrhythmias
and/or morphological changes.
[0154] The mean of the two control recordings was set to zero and
the effects recorded after consecutive doses of test substance were
expressed as percentage changes from this value. By plotting these
percentage values against the cumulative dose administered before
each recording, it was possible to construct dose-response curves.
In this way, each experiment generated three dose-response curves,
one for MAP duration, one for AV-conduction time and one for the
sinus frequency (RR interval). A mean curve of all experiments
performed with a test substance was calculated, and potency values
were derived from the mean curve. All dose-response curves in these
experiments were constructed by linear connection of the data
points obtained. The cumulative dose prolonging the MAP duration by
10% from the baseline was used as an index to assess the class III
electrophysiological potency of the agent under investigation
(D.sub.10).
Test B
Glucocorticoid-Treated Mouse Fibroblasts as a Model to Detect
Blockers of the Delayed Rectifier K Current
[0155] IC.sub.50 for K channel blockade was determined using a
microtitre plate based screen method, based on membrane potential
chances of glucocorticoid-treated mouse fibroblasts. The membrane
potential of glucocorticoid-treated mouse fibroblasts was measured
using fluorescence of the bisoxonol dye DiBac.sub.4(3), which could
be reliably detected using a fluorescence laser imaging plate
reader (FLIPR). Expression of a delayed rectifier potassium channel
was induced in mouse fibroblasts by 24 hours exposure to the
glucocorticoide dexamehasone (5 .mu.M). Blockade of these potassium
channels depolarised the fibroblasts, resulting in increased
fluorescence of DiBac.sub.4(3).
[0156] Mouse Itk fibroblasts (L-cells) were purchased from American
Type Culture Collection (ATCC, Manassa, Va.), and were cultured in
Dulbeccos modified eagle medium supplemented with fetal calf serum
(5% vol/vol), penicillin (500 units/mL), streptomycin (500
.mu.g/mL) and L-alanine-L-glutamine (0.862 mg/mL). The cells were
passaged every 3-4 days using trypsin (0.5 mg/mL in calcium-free
phosphate buffered saline, Gibco BRL). Three days prior to
experiments, cell-suspension was pipetted out into clear-bottom,
black plastic, 96-well plates (Costar) at 25 000 cells/well.
[0157] The fluorescence probe DiBac.sub.4(3) (DiBac Molecular
probes) was used to measure membrane potential. DiBac.sub.4(3)
maximally absorbs at 488 nM and emits at 513 in M. DiBac.sub.4(3)
is a bisoxonol, and thus is negatively charged at pH 7. Due to its
negative charge, the distribution of DiBac.sub.4(3) across the
membrane is dependent upon the transmembrane potential: if the cell
depolarizes (i.e. the cell interior becomes less negative relative
to cell exterior), the DiBac.sub.4(3) concentration inside the cell
increases, due to electrostatic forces. Once inside the cell,
DiBac.sub.4(3) molecules can bind to lipids and proteins, which
causes an increase in fluorescence emission. Thus, a depolarization
will be reflected by an increase in DiBac.sub.4(3) fluorescence.
The change in DiBac.sub.4(3) fluorescence was detected by a
FLIPR.
[0158] Prior to each experiment, the cells were washed 4 times in
phosphate-buffered saline (PBS) to remove all culture media. The
cells were then treated with 5 .mu.M DiBac.sub.4(3) (in 180 .mu.L
of PBS) at 35.degree. C. Once a stable fluorescence was reached
(usually after 10 min), 20 .mu.L of the test substance was added,
using FLIPR's internal 96 well pipetting system. Fluorescence
measurements were then taken every 20 sec for a further 10 min. All
experiments were carried out at 35.degree. C., due to the high
temperature sensitivity of both delayed rectifier potassium channel
conductance and DiBac.sub.4(3) fluorescence. Test substances were
prepared in a second 96 well plate, in PBS containing 5 .mu.M
DiBac.sub.4(3). The concentration of substance prepared was 10
times that of the desired concentration in the experiment as an
additional 1:10 dilution occurred during addition of substance
during the experiment. Dofetilide (10 .mu.M) was used as a positive
control, i.e. to determine the maximum increase in
fluorescence.
[0159] Curve-fitting, used to determine the IC50 values, was
performed with the Graphpad Prism program (Graphpad Software Inc.,
San Diego, Calif.).
Test C
Metabolic Stability of Test Compounds
[0160] An in vitro screen was set up to determine the metabolic
stability of the compounds of the invention.
[0161] The hepatic S-9 fraction from dog, man, rabbit and rat with
NADPH as co-factor was used. The assay conditions were as follows:
S-9 (3 mg/r/L), NADPH (0.83 mM), Tris-HCl buffer (50 mM) at pH 7.4
and 10 .mu.M of test compound.
[0162] The reaction was started by addition of test compound and
terminated after 0, 1, 5, 15 and 30 minutes by raising the pH in
the sample to above 10 (NaOH; 1 mM). After solvent extraction, the
concentration of test compound was measured against an internal
standard by LC (fluorescence/UV detection).
[0163] The percentage of test compound remaining after 30 minutes
(and thus t.sub.1/2) was calculated and used as a measure for
metabolic stability.
[0164] The invention is illustrated by way of the following
examples.
EXAMPLES
General Experimental Procedures
[0165] Mass spectra were recorded on one of the following
instruments: a Perkin-Elmer SciX API 150 ex spectrometer; a VG
Quattro II triple quadrupole; a VG Platform II single quadrupole;
or a Micromass Platform LCZ single quadrupole mass spectrometer
(the latter three instruments were equipped with a pneumatically
assisted electrospray interface (LC-MS)). .sup.1H NMR and .sup.13C
NMR measurements were performed on a BRUKER ACP 300 and Varian 300,
400 and 500 spectrometers, operating at .sup.1H frequencies of 300,
400 and 500 MHz respectively, and at .sup.13C frequencies of 75.5,
100.6 and 125.7 MHz respectively. Alternatively, .sup.13C NMR
measurements were performed on a BRUKER ACE 200 spectrometer at a
frequency of 50.3 MHz.
[0166] Rotamers may or may not be denoted in spectra depending upon
ease of interpretation of spectra. Unless otherwise stated,
chemical shifts are given in ppm with the solvent as internal
standard.
Synthesis of Intermediates
[0167] The following intermediates were not commercially available,
and were therefore prepared by the methods described below.
Preparation a
tert-Butyl 9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
hydrochloride
(i) 2,6-Bis(iodomethyl)-4-(phenylsulfonyl)morpholine
[0168] The sub-title compound was prepared according to both of the
following two methods:
[0169] (a) To a stirred mixture of water (835 mL), chloroform (1.25
L) and iodine (418.7 g, 1.65 mol) under an inert atmosphere
(N.sub.2) was added, portion-wise over a period of 30 min,
2,6-bis[(acetoxymercuri)methyl]-4-(phenylsulfonyl)morpholine
(prepared as described in Chem. Ber. 96, 2827 (1963); 421.3 g, 0.55
mol), during which time the reaction mixture was warmed to reflux.
After addition was complete, reflux was continued overnight before
the mixture was allowed to cool to room temperature. The mixture
was filtered and the chloroform layer separated. A saturated
aqueous solution of Na.sub.2S.sub.2O.sub.3 was added to the organic
solution until the iodine colour disappeared. The organic layer was
again separated, then dried (Na.sub.2SO.sub.4), filtered and
concentrated in vacuo to yield 279.9 g (100%) of the sub-title
compound as a light yellow crystalline solid. HPLC analysis
indicated this product to be composed of 46% cis-isomer and 54%
trans-isomer.
[0170] (b) Acetonitrile (50 mL) and then ether (150 mL) were added
to triphenylphosphine (20.1 g, 77 mmol). Imidazole (5.24 g, 77
mmol) was added and the solution cooled to 5.degree. C. Iodine
(19.5 g, 77 mmol) was added, causing the temperature to rise to
17.degree. C. A solution of
2,6-bis-(hydroxymethyl)-4-(phenylsulfonyl)morpholine (Preparation
L; 10.65 g, 37 mmol) in acetonitrile (50 mL) was added and the
reaction stirred at room temperature for 22 hours. Aqueous sodium
thiosulfate (5%, 100 mL) was added and the layers separated. The
organic phase was washed with dilute sulfuric acid (100 mL) and
then was concentrated under reduced pressure. The residue was
purified by chromatography over silica (200 g), eluting with
dichloromethane (1.5 L), to give a yellow oil. This was triturated
with ether to give the title compound as a yellow solid (a 1:1
mixture of cis- and trans-isomers; 6.1 g, 37%). The ether wash
contained impure product (1.90 g).
[0171] API MS: m/z=508
[C.sub.12H.sub.15I.sub.2NO.sub.3S+H].sup.+.
(ii)
3-Benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane
[0172] A mixture of benzylamine (173.3 g, 1.62 mol), cis- and
trans-2,6-bis(iodomethyl)-4-(phenylsulfonyl)morpholine (from step
(i)(a) above; 275 g, 0.54 mol), and sodium hydrogencarbonate (182.2
g, 2.17 mol) in acetonitrile (13.5 L) was refluxed for 24 h. After
this time, an aliquot was removed and diluted with ethyl acetate.
HPLC analysis of this sample indicated that approximately 9% of the
cis-isomer of the starting material remained unreacted. Reflux was
continued for a further 6 h, but this gave no change in the
percentage of unreacted starting material (as indicated by HPLC).
The reaction was then allowed to cool to rt before the mixture was
filtered and the filtrate concentrated in vacuo. The resulting
crude product was partitioned between dichloromethane and 0.5 N
NaOH solution. The organic layer was separated, washed with brine,
dried (Na.sub.2SO.sub.4), filtered and then concentrated in vacuo
to afford a mixture of oil and crystals. This mixture was slurried
in toluene (300 mL) and the crystalline product collected by
filtration. The filter cake of crystals was rinsed with cold
toluene (100 mL), and then dried in a vacuum oven overnight
(40.degree. C., 13.3 Pa (0.1 mmHg)) to give 61.7 g (31.9% yield,
72.8% conversion of the cis-isomer) of the sub-title compound.
[0173] API MS: m/z=359
[C.sub.19H.sub.22.sup.-N.sub.2O.sub.3S+H].sup.+.
(iii) 3-Benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane
dihydrochloride
[0174] To a mixture of anhydrous THF (1.1 L) and pellets of
LiAlH.sub.4 (48.5 g, 1.2 mol) under an inert atmosphere (N.sub.2)
was added
3-benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane
(from step (ii) above; 61.7 g, 0.17 mol) in portions over a 30 min
period. The mixture was refluxed for 48 h before being cooled to
-10.degree. C. The cooled mixture was then treated sequentially
(and cautiously) with water (45.8 mL), 15% is NaOH solution (45.8
mL) and then water (137.4 mL) again. The resulting mixture was
filtered through Celite.RTM. and the filtrate set aside. The
inorganic salts from the filter cake were transferred to a beaker
and stirred with ethyl acetate (1 L) for 30 min. This slurry was
then filtered through Celite.RTM. again. The two filtrates were
combined and then concentrated in vacuo to afford an oil (32.2 g).
This oil was dissolved in methanol (120 mL) and treated with a
solution of HCl in IPA (100 mL), after which the solution pH was
checked for acidity. After standing for 24 h, a crop of crystals
was collected by filtration and dried to a constant weight of 26.8
g. A second crop of crystals (7 g) was later obtained by
crystallisation of the remaining crude product from IPA, giving a
total yield of 33.8 g (68%) of the sub-title compound.
[0175] .sup.1H NMR (CD.sub.3OD+4 drops D.sub.2O): .delta. 2.70 (br
d, 2H), 3.09 (d, 2H), 3.47 (br s, 4H), 3.60 (s, 2H), 4.12 (br s,
2H), 7.30-7.45 (m, 5H).
[0176] API MS: m/z=219 [C.sub.13H.sub.18N.sub.2O+H].sup.+.
(iv) tert-Butyl
7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
[0177] A mixture of water (400 mL), dichloromethane (400 mL),
sodium hydrogencarbonate (40.3 g, 0.48 mol) and
3-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane dihydrochloride (from
step (iii) above; 33.7 g, 0.12 mol) was stirred rapidly for 10 min
before di-tert-butyl dicarbonate (27.8 g, 0.13 mol) was added in
portions. After addition was complete, the reaction was stirred for
a further 2 h. The organic layer was separated, dried
(Na.sub.2SO.sub.4), filtered and concentrated in vacuo to afford
39.6 g of an off-white crystalline solid. This material was used
directly in the next step without any further purification.
[0178] .sup.1H NMR (CD.sub.3OD): .delta. 1.5 (s, 9H), 2.42 (br t,
2H), 2.88 (d, 2H), 3.18-3.28 (m, 2H), 3.38 (d, 2H), 3.80 (br d,
2H), 4.00 (d, 2H), 7.16-7.38 (m, 5H).
(v) tert-Butyl
7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
hydrochloride
[0179] A solution of tert-butyl
7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate (39.5 g,
0.12 mol) in ethyl acetate (200 mL) was cooled to -10.degree. C.
under an inert atmosphere (N.sub.2). A solution of HCl in diethyl
ether (1 M) was added over the course of 1 h, during which time a
precipitate formed. After addition was complete, the resulting
mixture was stirred for a further 1 h before the crystalline
precipitate was collected by filtration and dried in a vacuum oven
(40.degree. C., 13.3 Pa (0.1 mmHg)). This gave 42.6 g (100% from
the compound of step (iii) above) of the sub-title compound as an
off-white crystalline material.
[0180] API MS: m/z=219
[C.sub.18H.sub.26N.sub.2O.sub.3--C.sub.5H.sub.9O.sub.2].sup.+.
(vi) tert-Butyl 9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
hydrochloride
[0181] A mixture of tert-butyl
7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
hydrochloride (from step (vi) above; 42.6 g, 0.12 mol), 10%
palladium on carbon (2.5 g) and methanol (450 mL) was subjected to
hydrogenation at atmospheric pressure. Once the reaction was
complete (as indicated by tlc), the mixture was filtered and the
filtrate concentrated in vacuo to yield 31.6 g of an off-white
crystalline product. This crude product was dissolved in hot
acetonitrile (450 mL), filtered and the filtrate diluted with ethyl
acetate (450 mL). After being allowed to stand at rt for 6 h, the
mixture was filtered to remove the first crop of crystallised
product (19.8 g). The mother liquor was then concentrated to near
dryness to give a residue that was dissolved in hot acetonitrile
(150 mL). Ethyl acetate (150 mL) was added to this solution and the
mixture allowed to stand at room temperature overnight. A second
crop of crystalline product (8.7 g) was then collected by
filtration, and was found to have an identical .sup.1H NMR spectrum
and melting point to the first crop. The combined yield of title
compound was therefore 28.5 g (89%).
[0182] m.p.=207-208.degree. C.
Preparation B
4-{[(2S)-2-Hydroxy-3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]-oxy}be-
nzonitrile
(i) 4-[(2S)-Oxiranylmethoxy]benzonitrile
[0183] Potassium carbonate (414 g) and (R)-(-)-epichlorohydrin (800
mL) were added to a stirred solution of p-cyanophenol (238 g) in
2.0 L MeCN and the reaction mixture was refluxed under an inert
atmosphere for 2 h. The hot solution was filtered and the filtrate
concentrated, giving a clear oil which was crystallised from
di-iso-propyl ether giving the product in 90% yield.
(ii) tert-Butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate
[0184] A mixture of tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate (prepared in an
analogous fashion to the compound of Preparation A(vi) above; 0.72
g, 3.2 mmol) and 4-[(2S)-oxiranylmethoxy]benzonitrile (from step
(i) above; 0.56 g, 3.2 mmol) in IPA/water (11 mL of 10:1) was
stirred at 60.degree. C. for 18 h. The solvent was then evaporated
to give 1.3 g (100%) of the sub-title compound, which was used in
the next step without further purification.
(iii)
4-{[(2S)-2-Hydroxy-3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]--
oxy}benzonitrile
[0185] A solution of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.11-
]nonane-3-carboxylate (from step (ii) above; 1.0 g, 2.47 mmol) in
ethyl acetate (13 mL) was cooled to 0.degree. C. Ethyl acetate (26
mL) saturated with gaseous HCl was added, and the mixture stirred
for 4 h at rt. The solvent was removed in vacuo before MeCN (25
mL), water (1.3 mL) and K.sub.2CO.sub.3 (2.0 g) were added. The
resulting mixture was stirred overnight before CHCl.sub.3 was
added, and the mixture filtered through Celite.RTM.. The filtrate
was concentrated in vacuo to give 682 mg (91%) of the title
compound.
Preparation C
4-{[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]amino}benzonitrile
(i) 4-[(3-Hydroxypropyl)amino]benzonitrile
[0186] A mixture of 4-fluorobenzonitrile (12.0 g, 99.1 mmol) and
3-amino-1-propanol (59.6 g, 793 mmol) was stirred at 80.degree. C.
under an inert atmosphere for 3 hours before water (150 mL) was
added. The mixture was allowed to cool to rt, and was then
extracted with diethyl ether. The organic layer was separated,
dried (Na.sub.2SO.sub.4), filtered and concentrated in vacuo to
yield 17 g (97%) of the title compound as an oil that crystallised
upon standing.
(ii) 3-(4-Cyanoanilino)propyl 4-methylbenzenesulfonate
[0187] A cooled (0.degree. C.) solution of
4-[(3-hydroxypropyl)amino]benzonitrile (from step (i) above; 17 g,
96.5 mmol) in dry MeCN (195 mL) was treated with triethylamine (9.8
g, 96.5 mmol), and then p-toluenesulfonyl chloride (20.2 g, 106
mmol). The mixture was stirred at 0.degree. C. for 90 minutes
before being concentrated in vacuo. Water (200 mL) was added to the
residue, and the aqueous solution was extracted with DCM. The
organic phase was dried (Na.sub.2SO.sub.4), filtered and
concentrated in vacuo. The resulting residue was purified by
crystallisation from iso-propanol to yield 24.6 g (77%) of the
sub-title compound.
(iii) tert-Butyl
7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-3-carbo-
xylate
[0188] The hydrochloride salt of tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate (from Preparation
A(vi) above; 1.1 g, 4.15 mmol) was mixed with MeCN (46 mL), water
(2.5 mL) and K.sub.2CO.sub.3 (3.5 g, 25 mmol). The mixture was
stirred for 4 h before CHCl.sub.3 was added and the mixture was
filtered through Celite.RTM.. The filtrate was concentrated in
vacuo to give 0.933 g of the free base. This was then mixed with
3-(4-cyanoanilino)propyl 4-methylbenzenesulfonate (from step (ii)
above; 2.1 g, 6.2 mmol) and K.sub.2CO.sub.3 (0.86 g, 6.2 mmol) in
MeCN (18 mL). The resulting mixture was stirred overnight at
60.degree. C. before being concentrated in, vacuo. The residue was
treated with DCM (250 mL) and 1 M NaOH (50 mL). The layers were
separated and the DCM layer washed twice with aqueous NaHCO.sub.3,
before being dried (Na.sub.2SO.sub.4) and concentrated in vacuo.
The product was purified by flash chromatography, eluting with a
gradient of toluene:ethyl acetate:triethylamine (2:1:0 to
1000:1000:1), to give 1.47 g (91%) of the sub-title compound.
(iv)
4-{[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]amino}-benzonitri-
le
[0189] The title compound was obtained in 96% yield according to
the procedure described in Preparation B(iii) above, using
tert-butyl
7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carbox-
ylate (from step (iii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation D
4-[2-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]benzonitrile
(i) 4-(2-Bromoethoxy)benzonitrile
[0190] A mixture of 4-cyanophenol (35.7 g, 0.3 mol),
K.sub.2CO.sub.3 (41.4 g, 0.3 mol) and 1,2-dibromoethane (561 g, 3.0
mol) in MeCN (450 mL) was stirred under reflux overnight. The
mixture was filtered and evaporated to give 30.2 g (45%) of the
sub-title compound, which was used without further
purification.
(ii) tert-Butyl
7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-3-carbox-
ylate
[0191] The sub-title compound was prepared in 85% yield according
to the procedure described in Preparation C(iii) above, using
4-(2-bromoethoxy)-benzonitrile (0.8 g, 3.5 mmol, 1.03 eq.) and
triethylamine (1.5 eq.) in place of 3-(4-cyanoanilino)propyl
4-methylbenzenesulfonate, and K.sub.2CO.sub.3, respectively.
(iii)
4-[2-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]benzonitrile
[0192] The title compound was obtained in 95% yield according to
the procedure described in Preparation B(iii) above, using
tert-butyl
7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxy-
late (from step (ii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation E
4-[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propoxy]benzonitrile
(i) 4-(3-Bromopropoxy)benzonitrile
[0193] 1,3-Dibromopropane (1.02 L, 10 mol) was added to a stirred
suspension of p-cyanophenol (238 g, 2 mol), K.sub.2CO.sub.3 (276.4
g, 2 mol) in MeCN (2.7 L). The reaction mixture was refluxed for 4
h, filtered and concentrated. The residue was recrystallised from
iso-propyl ether to give the sub-title compound in a 69% yield.
(ii) tert-Butyl
7-[3-(4-cyanophenoxy)propyl]-9-oxa-3,7-diazabicyclo-[3.3.1]nonane-3-carbo-
xylate
[0194] The sub-title compound was prepared in 97% yield according
to the procedure described in Preparation C(iii) above, using
4-(3-bromo-propoxy)benzonitrile (from step (i) above) in place of
3-(4-cyanoanilino)-propyl 4-methylbenzenesulfonate.
(iii)
4-[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propoxy]benzonitrile
[0195] The title compound was obtained in 90% yield according to
the procedure described in Preparation B(iii) above, using
tert-butyl
7-[3-(4-cyanophenoxy)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carbox-
ylate (from step (ii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation F
4-[2-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]isophthalonitrile
(i) 4-(2-Bromoethoxy)isophthalonitrile
[0196] The sub-title compound was prepared in 64% yield according
to the procedure described in Preparation D(i) above, using
4-hydroxy-isophthalonitrile in place of 4-cyanophenol.
(ii) tert-Butyl
7-[2-(2,4-dicyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo-[3.3.1]nonane-3-ca-
rboxylate
[0197] The sub-title compound was prepared in 62.7% yield according
to the procedure described in Preparation C(iii) above, using
4-(2-bromoethoxy)-isophthalonitrile (from step (i) above) in place
of 3-(4-cyanoanilino)propyl 4-methylbenzenesulfonate.
(iii)
4-[2-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]isophthalonitrile
[0198] The title compound was obtained according to the procedure
described in Preparation B(iii) above, using tert-butyl
7-[2-(2,4-dicyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-car-
boxylate (from step (ii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation G
4-[4-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)butyl]benzonitrile
(i) 4-(3-Butenyl)benzonitrile
[0199] Magnesium (4.2 g, 173 mmol) was activated by washing with
dilute HCl, water and acetone and was then dried in vacuo.
ZnBr.sub.2 (37 g, 165 mmol) was sublimed under reduced pressure
(approx. 5-20 mmHg) by gentle heating in a glovebox. The glovebox
was used as it was extremely humid in the lab. Mg and ZnBr.sub.2
were mixed in a dry 3-necked flask under N.sub.2 and dry THF (30
mL) was added. 4-Bromo-1-butene (25.1 g, 186=mol) dissolved in dry
THF (175 mL) was added dropwise to the Mg/ZnBr.sub.2 slurry and,
during the addition, the reaction mixture turned greyish and then
black. Some heat was also evolved (>40.degree. C.). After
complete addition, the mixture was heated to 50.degree. C.
overnight. 4-Bromobenzonitrile (30.5 g, 167 mmol) was co-evaporated
with toluene twice and was then dissolved in dry THF (250 ml)
together with Pd(PPh.sub.3).sub.4 (5 g, 4.3 mmol, 2.5 mole). The
slurry was added to the Grignard reagent and the reaction mixture
was stirred at room temperature overnight. HCl (500 mL, 3 M) was
added dropwise to the reaction mixture, and the resulting solution
was extracted with ether (1000+3.times.500 mL), the combined ether
solutions were washed with NaHCO.sub.3 (satd. 3.times.250 mL),
dried, filtered and evaporated. The crude product (29.7 g) was
subjected to Dry-Flash chromatography (diameter 12 cm, height 5 cm,
heptane:EtOAc (99:1 to 90:10)) to give 21.2 g of the sub-title
compound contaminated with 4-bromobenzonitrile (about 20%). This
material was used in the next step.
(ii) 4-(4-Hydroxybutyl)benzonitrile
[0200] 4-(3-Butenyl)benzonitrile (from step (i) above, 10.8 g, 69
mmol) was dissolved in dry THF (140 mL) and was cooled to 0.degree.
C. BH.sub.3-Me.sub.2S complex (20 mL, 2 M) was added dropwise over
45 minutes at 0.degree. C. and, after 7 hours, water (70 mL) and
NaBO.sub.3-4H.sub.2O (25 g) were added and the mixture was stirred
overnight before dilution with ether (700 mL) and brine (satd., 250
mL). After separation, the aqueous phase was extracted with ether
(2.times.200 mL) and the combined extracts were dried, filtered and
evaporated to give crude sub-title compound. Purification by flash
chromatography on SiO.sub.2 (300 g) with heptane:EtOAc (3:1 to 1:1)
gave the sub-title compound (6.99 g).
(iii) 4-(4-Cyanophenyl)butyl methanesulfonate
[0201] Methanesulfonyl chloride (2.32 mL, 30 mmol) was added to a
cooled (0.degree. C.), stirred solution of
4-(4-hydroxybutyl)benzonitrile (from step (ii) above, 5.2 g, 29.7
mmol) and triethylamine (4.35 mL) in dichloromethane (50 mL). The
resulting mixture was stirred for 4 h before water (150 mL) was
added, and the organic layer separated, dried and concentrated to
give the sub-title compound. This product was used directly in the
next step without further purification.
(iv) tert-Butyl
7-[4-(4-Cyanophenyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-3-carboxy-
late
[0202] The sub-title compound was prepared in 69.3% yield according
to the procedure described in Preparation C(iii) above, using
4-(4-cyanophenyl)-butyl methanesulfonate (from step (iii) above) in
place of 3-(4-cyanoanilino)propyl 4-methylbenzenesulfonate.
(v)
4-[4-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)butyl]benzonitrile
[0203] The title compound was obtained in 88% yield according to
the procedure described in Preparation B(iii) above, using
tert-butyl
7-[4-(4-cyanophenyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxyl-
ate (from step (iv) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation H
4-[1-(3,4-Dimethoxyphenoxy)-4-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)butyl-
]benzonitrile
(i) 4-[1-(3,4-Dimethoxyphenoxy)-3-butenyl]benzonitrile
[0204] A cooled (0.degree. C.) mixture of
4-(1-hydroxy-3-butenyl)benzonitrile (14.6 g, 84.3 mmol) and
3,4-dimethoxyphenol (19.5 g, 125.4 mmol) in toluene is (500 mL) was
treated with tributylphosphine (32.14 mL of 97% purity, 25.6 g,
126.4 mmol), followed by 1,1'-(azodicarbonyl)dipiperidine (31.8 g,
126.4 mmol). After addition was complete, the reaction mixture
thickened and the temperature rose to 15.degree. C. Additional
toluene was added (500 mL), and the mixture stirred at rt
overnight. The precipitate of tributylphosphine oxide was then
removed by filtration and the filtrate concentrated in vacuo to
give 65.8 g of crude product. This was purified by chromatography
on silica gel, eluting with toluene:methanol (98:2), to yield 17.9
g of the sub-title compound.
(ii) 4-[1-(3,4-Dimethoxyphenoxy)-4-hydroxybutyl]benzonitrile
[0205] Borane-methyl sulfide complex (2 M in ether, 11 mL, 22 mmol)
was added dropwise to a cooled (-5.degree. C.) solution of
4-[1-(3,4-dimethoxyphenoxy)-3-butenyl]benzonitrile (from step (i)
above; 17.6 g, 56.8 mmol) in dry THF (15 mL) over a period of 15
minutes (during which time the reaction temperature rose to
0.degree. C.). The resulting mixture was stirred at between 0 and
10.degree. C. for 1.5 h, before being allowed to warm to rt.
Stirring was continued for a further 3.5 h at this temperature
before water (22 mL) and sodium perborate tetrahydrate (11 g, 66
mmol) were added. The biphasic mixture was stirred for 2 h at rt
before the water layer was separated and extracted with ether. The
combined organic layers were washed with brine, dried and
concentrated in vacuo. The resulting residue was purified by
chromatography on silica gel, eluting with IPA:ethyl
acetate:heptane (5:25:70) to yield 14.5 g (77%) of the sub-title
compound.
(iii) 4-(4-Cyanophenyl)-4-(3)-4-dimethoxyphenoxy)butyl
methanesulfonate
[0206] A solution of methanesulfonyl chloride (3.4 mL, 5.0 g, 44
mmol) in DCM (15 mL) was added slowly to a cooled (-5.degree. C.)
mixture of 4-[1-(3,4-dimethoxyphenoxy)-4-hydroxybutyl]benzonitrile
(from step (ii) above; 11 g, 34 mmol) and triethylamine (7 mL, 5.2
g, 50.6 mmol) in DCM (50 mL), during which addition the temperature
did not rise above 2.degree. C. Stirring was continued at between 0
and 5.degree. C. for a further 2 h before water was added. The
resulting organic layer was separated, and washed with water,
separated again and then dried to give the sub-title compound in
100% yield.
(iv) tert-Butyl
7-[4-(4-cyanophenyl)-4-(3,4-dimethoxyphenoxy)butyl]-9-oxa-3,7-diazabicycl-
o[3.3.1]nonane-3-carboxylate
[0207] The sub-title compound was prepared in 82% yield according
to the procedure described in Preparation C(iii) above, using
4-(4-cyanophenyl)-4-(3,4-dimethoxyphenoxy)butyl methanesulfonate
(from step (iii) above) in place of 3-(4-cyanoanilino)propyl
4-methylbenzenesulfonate.
(v)
4-[1-(3,4-Dimethoxyphenoxy)-4-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)b-
utyl]benzonitrile
[0208] The title compound was obtained in quantitative yield
according to the procedure described in Preparation B(iii) above,
using tert-butyl
7-[4-(4-cyanophenyl)-4-(3,4-dimethoxyphenoxy)butyl]-9-oxa-3,7-diazabicycl-
o-[3.3.1]nonane-3-carboxylate (from step (iv) above) in place of
tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo-[3.3.1-
]nonane-3-carboxylate.
Preparation I
4-{[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]sulfonyl}benzonitrile
(i) 4-[(3-Bromopropyl)sulfanyl]benzonitrile
[0209] A mixture of 4-cyanothiophenol (20.8 g, 154 mmol),
1,3-dibromopropane (155 g, 0.77 mol) and K.sub.2CO.sub.3 (21.3 g,
154 mmol) in MeCN (300 mL) was refluxed overnight. Filtration and
evaporation of the solvent gave a brown oil that crystallised when
treated with EtOH. The crystals were isolated by filtration to give
the sub-title compound (24.5 g, 62%).
(ii) 4-[(3-Bromopropyl)sulfonyl]benzonitrile
[0210] 3-Chloroperoxybenzoic acid (44.9 g of 70%, 182 mmol) was
added slowly to a cooled (0.degree. C.) solution of
4-[(3-bromopropyl)sulfanyl]benzonitrile (from step (i) above; 23.4
g, 91 mmol) in DCM (250 mL). The mixture was then stirred at rt
overnight-, and the resulting precipitate filtered off. The
filtrate was concentrated in vacuo to give a residue that was shown
(by NMR analysis) to contain 25% sulfoxide in addition to the
desired product. The residue was redissolved in DCM (250 mL),
additional 3-chloroperoxybenzoic acid (5.6 g of 70%, 23 mmol)
added, and the mixture stirred for 30 min. Dimethylsulfoxide (20
mmol) was added to destroy excess mCPBA before the DCM solution was
washed with aqueous NaHCO.sub.3, separated, dried and concentrated
in vacuo. This gave the sub-title compound in 76% yield.
(iii) tert-Butyl
7-{3-[(4-cyanophenyl)sulfonyl]propyl}-9-oxa-3,7-diazabicyclo[3.3.1]nonane-
-3-carboxylate
[0211] The sub-title compound was prepared in 64% yield according
to the procedure described in Preparation C(iii) above, using
4-[(3-bromopropyl)sulfonyl]benzonitrile (from step (ii) above) in
place of 3-(4-cyanoanilino)propyl 4-methylbenzenesulfonate.
(iv)
4-{[3-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]sulfonyl}-benzoni-
trile
[0212] The title compound was obtained in 84% yield according to
the procedure described in Preparation B(iii) above, using
tert-butyl
7-{3-[(4-cyanophenyl)sulfonyl]propyl}-9-oxa-3,7-diazabicyclo[3.3.1]nonane-
-3-carboxylate (from step (iii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
Preparation J
tert-Butyl
(1S)-2-(4-cyanophenoxy)-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-y-
lmethyl)ethylcarbamate
(i) 4-(2-Oxiranylmethoxy)benzonitrile
[0213] The sub-title compound was prepared in 75% yield according
to the procedure described in Preparation B(i) above, using
epichlorohydrin in place of (R)-(-)-epichlorohydrin.
(ii) 4-[(3-Amino-2-hydroxypropyl)oxy]benzonitrile
[0214] 4-(Oxiranylmethoxy)benzonitrile (from step (i) above; 100 g,
0.57 mol) was added to a mixture of concentrated aqueous ammonium
hydroxide (500 mL) and iso-propanol (300 mL). The resulting slurry
was stirred at room temperature for 3 days. The reaction mixture
was filtered to remove the insoluble by-product, and the filtrate
was concentrated in vacuo to give a crude product, which was
crystallised from acetonitrile to yield 50 g (46%) of the sub-title
compound.
(iii) tert-Butyl 3-(4-cyanophenoxy)-2-hydroxypropylcarbamate
[0215] A cooled (0.degree. C.) solution of
4-[(3-amino-2-hydroxypropyl)oxy]benzonitrile (from step (ii) above;
44.6 g, 0.23 mol) in THF:H.sub.2O (1.5 L of 1:1) was treated with
di-tert-butyl dicarbonate (53 g, 0.24 mol). The mixture was stirred
at rt overnight, after which NaCl was added and the resulting is
organic layer separated. The water layer was extracted with ether
and the combined organics were dried and concentrated in vacuo. The
resulting oil (70 g) was filtered through a plug of silica, and
then crystallised from diethyl ether: di-iso-propyl ether to yield
50 g of the sub-title compound.
(iv) 2-[(tert-Butoxycarbonyl)amino]-1-[(4-cyanophenoxy)methyl]ethyl
methanesulfonate
[0216] Methanesulfonyl chloride (22.3 g 0.195 mol) was added over
the course of 1.5 hours to a cooled (0.degree. C.) solution of
tert-butyl 3-(4-cyano-phenoxy)-2-hydroxypropylcarbamate (from step
(iii) above; 51.2 g, 0.177 mol) and 4-(dimethylamino)pyridine (1.3
g, 10.6 mmol) in pyridine (250 mL), kept under an inert atmosphere.
The reaction mixture was stirred for 2 h at rt before water and DCM
were added. The organic layer was separated, washed with water,
dried (MgSO.sub.4) and concentrated in vacuo to yield 68.1 g (100%)
of the sub-title compound.
(v) tert-Butyl
2-[(4-cyanophenoxy)methyl]-1-aziridinecarboxylate
[0217] A cooled (0.degree. C.) solution of
2-[(tert-butoxycarbonyl)amino]-1-[(4-cyanophenoxy)methyl]ethyl
methanesulfonate (from step (iv) above; 30.6 g, 82.6 mmol) and
tetrabutylammonium hydrogensulfate (3 g, 8.8 mmol) in DCM (100 mL)
was treated with 50 wt. % aqueous NaOH (60 mL) under an inert
atmosphere. The resulting mixture was stirred, and the temperature
was slowly allowed to rise to rt over for 4 h, and then extracted
with ether. The organic layer was washed with water and
concentrated in vacuo to give a residue that was purified by column
chromatography (dichloromethane eluent). Crystallisation from
diethyl ether: di-iso-propyl ether gave the sub-title compound in
quantitative yield.
(vi) tert-Butyl
(2S)-2-[(4-cyanophenoxy)methyl]-1-aziridinecarboxylate
[0218] The sub-title compound was prepared according to the
procedures described in steps (i) to (v) above for the synthesis of
tert-butyl 2-[(4-cyanophenoxy)methyl]-1-aziridinecarboxylate, but
using (S)-(+)-epichlorohydrin in place of epichlorohydrin in step
(i).
(vii) 3-Benzyl-7-(tert-butyl)
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3,7-dicarboxylate
[0219] The hydrochloride salt of tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate (from Preparation
A(vi) above; 2.17 g, 8.2 mmol) was dissolved in CHCl.sub.3 (25 mL)
and the mixture was cooled to 0.degree. C. Triethylamine (2.1 g,
20.6 mmol) was added, followed by
N-(benzyloxycarbonyloxy)succinimide (2.24 g. 9.0 mmol), and the
mixture stirred at rt for 24 h. The reaction mixture was washed
with water (4.times.15 mL), before the organic layer was separated,
dried (MgSO.sub.4) and concentrated in vacuo. This gave the
sub-title compound (4.4 g, containing some TEA) which was used in
the next step without further purification.
(viii) Benzyl 9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
[0220] The title compound was obtained in 55% yield according to
the procedure described in Preparation B(iii) above, using
3-benzyl-7-(tert-butyl)
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3,7-dicarboxylate (from step
(vii) above) in place of tert-butyl
7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
nonane-3-carboxylate.
(ix) Benzyl
7-[(2S)-2-[(tert-butoxycarbonyl)amino]-3-(4-cyanophenoxy)-propyl]-9-oxa-3-
,7-diazabicyclo[3.3.1]nonane-3-carboxylate
[0221] The sub-title compound was obtained in 71% yield according
to the procedure described in Preparation B(ii) above, using benzyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate (from step (viii)
above) and tert-butyl
(2S)-2-[(4-cyanophenoxy)methyl]-1-aziridinecarboxylate (from step
(vi) above) in place of tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate and
4-[(2S)-oxiranylmethoxy]benzonitrile, respectively.
(x) tert-Butyl
(1S)-2-(4-cyanophenoxy)-1-(9-oxa-3,7-diazabicyclo[3.3.1]-non-3-ylmethyl)e-
thylcarbamate
[0222] A solution of benzyl
7-[(2S)-2-[(tert-butoxycarbonyl)amino]-3-(4-cyanophenoxy)propyl]-9-oxa-3,-
7-diazabicyclo[3.3.1]nonane-3-carboxylate (from step (ix) above;
2.55 g, 4.7 mmol) in 95% ethanol (50 mL) was hydrogenated over 5%
Pd/C (0.8 g) at 30 kPa. When the quantity of hydrogen calculated
for complete reaction had been consumed, the reaction was stopped.
The mixture was filtered through Celite.RTM., and the filtrate
concentrated in vacuo. The resulting residue was purified by
chromatography on silica, eluting with CHCl.sub.3:ammoniacal
methanol (95:5), to yield the title compound 1.39 g (75%).
Preparation K
4-{[2-Hydroxy-3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]oxy}-benzoni-
trile
[0223] The title compound was prepared according to the method
described in preparation B above, using epichlorohydrin in place of
(R)-(-)-epichlorohydrin in step (i).
Preparation L
2,6-Bis(hydroxymethyl)-4-(phenylsulfonyl)morpholine
(i) N,N-Bis(2-oxiranylmethyl)benzenesulfonamide
[0224] This reaction is very exothermic so care must be taken if
the reaction is scaled up. Acetonitrile (400 mL) and
(.+-.)-epichlorohydrin (100 mL, 118.3 g, 0.78 mol) were added to
benzenesulfonamide (50.0 g, 0.32 mol), followed by cesium carbonate
(228 g, 0.70 mol). The mixture was heated at reflux for 15 hours
with mechanical stirring. After cooling to room temperature water
was added (250 mL) and the organic phase separated and concentrated
under reduced pressure. The residual oil was chromatographed over
silica (300 g), eluting with dichloromethane (1 L) and then
dichloromethane:ethyl acetate (3 L of 19:1), to give the sub-title
compound as an oil (39.4 g, 46%).
[0225] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 2.55-2.65 (2H,
m), 2.79 (2H, t, J 4.4), 3.10-3.22 (4H, m), 3.58-3.73 (2H, m),
7.50-7.56 (2H, m), 7.58-7.63 (1H, m), 7.83-7.87 (2H, m).
(ii) 2,6-Bis(hydroxymethyl)-4-(phenylsulfonyl)morpholine
[0226] Tetrahydrofuran (40 mL) was added to
N,N-bis(2-oxiranylmethyl)benzenesulfonamide (from step (i)
(alternative A) above; 10 g, 37.1 mmol), followed by dilute
sulfuric acid (10 mL of 1 M), and the mixture stirred for 6 days
(reaction is complete within 1 day). Solid sodium chloride (3 g)
and ethyl acetate (40 mL) were added, and the mixture stirred for 1
hour. The organic phase was separated and washed with aqueous
ammonium chloride (10 mL of 10%). The organic phase was
concentrated under reduced pressure before toluene was added (50
mL). The mixture was concentrated again to leave the title compound
as a crude oil (10.65 g). This material was employed directly in
subsequent reactions without any further purification.
Preparation M
cis-2,6-Bis(hydroxymethyl)-4-(phenylsulfonyl)morpholine
(i) Chirally enriched
N,N-bis(2-oxiranylmethyl)benzenesulfonamide
[0227] This reaction is very exothermic so care must be taken if
the reaction is scaled up. Acetonitrile (100 mL) and
(R)-(-)-epichlorohydrin (47 mL, 55.6 g, 0.60 mol) were added to
benzenesulfonamide (20.0 g, 0.127 mol), followed by cesium
carbonate (83 g, 0.255 mol). The mixture was heated at reflux for 6
hours with mechanical stirring and then was stirred overnight at
room temperature. Water was added (100 mL), the organic phase
separated and then concentrated under reduced pressure. The
residual oil was chromatographed over silica, eluting with
dichloromethane then dichloromethane:ethyl acetate (19:1), to give
the sub-title compound as an oil (14.8 g, 43%).
(ii) cis-2,6-Bis(hydroxymethyl)-4-(phenylsulfonyl)morpholine
[0228] Tetrahydrofuran (60 mL) was added to chirally-enriched
N,N-bis(2-oxiranylmethyl)benzenesulfonamide (from step (i) above;
14.8 g, 55 mmol), followed by dilute sulfuric acid (15 mL of 1 M),
and the mixture stirred for 3 days. Solid sodium chloride (11 g)
was added and the mixture stirred for 1 hour. The organic phase was
separated and concentrated under reduced pressure to give crude
product (22.4 g). The material was purified by column
chromatography over silica, eluting with dichloromethane:ethanol
(19:1), to give the title compound (4 g, 25%) and an impure
fraction that was a 2:1 mixture of cis- and trans-isomers (8 g,
75%).
[0229] The cis-isomer is also ultimately formed as the major
product if (S)-(+)-epichlorohydrin is used in step (i) in place of
(R)-(-)-epichlorohydrin.
Preparation N
3,3-Dimethyl-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-2-butanone
(i) N,N-Bis(2-oxiranylmethyl)benzenesulfonamide
[0230] The following is an alternative preparation to that
described in Preparation L(i) above.
[0231] Water (2.5 L, 10 vol.) followed by epichlorohydrin (500 mL,
4 eq.) were added to benzenesulfonamide (250 g, 1 eq.). The
reactants were heated to 40.degree. C. Aqueous sodium hydroxide
(130 g in 275 mL of water) was added such that the temperature of
the reaction remained between 40.degree. C. and 43.degree. C. This
took approximately 2 hours. (The rate of sodium hydroxide addition
needs to be slower at the start of the addition than at the end in
order to keep within the temperature range stated.) After the
addition of sodium hydroxide was complete, the reaction was stirred
at 40.degree. C. for 2 hours, then at ambient temperature
overnight. The excess epichlorohydrin was removed as a water
azeotrope by vacuum distillation (ca. 40 mbar, internal temp
30.degree. C.), until no more epichlorohydrin distilled.
Dichloromethane (1 L) was added and the mixture stirred rapidly for
15 minutes. The phases were allowed to separate (this took 10
minutes although totally clear phases are obtained after standing
overnight). The phases were separated and the dichloromethane
solution used in the subsequent step below.
[0232] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 2.55-2.65 (2H,
m), 2.79 (2H, t, J 4.4), 3.10-3.22 (4H, m), 3.58-3.73 (2H, m),
7.50-7.56 (2H, m), 7.58-7.63 (1H, m), 7.83-7.87 (2H, m).
(ii)
5-Benzyl-3,7-dihydroxy-1-phenylsulfonyl-1,5-diazacyclooctane
[0233] IMS (2.5 L, 10 vol) was added to the dichloromethane
solution from step (i) above. The solution was distilled until the
internal temperature reached 70.degree. C. Approximately 1250 mL of
solvent was collected. More IMS (2.5 L, 10 vol) was added followed
by benzylamine (120 mL, 0.7 eq.) in one portion (no exotherm seen),
and the reaction was heated at reflux for 6 hours (no change from 2
hour sampling point). More benzylamine was added (15 mL) and the
solution was heated for a further 2 hours. The IMS was distilled
off (ca. 3.25 L) and toluene was added (2.5 L). More solvent was
distilled (ca. 2.4 L) and then further toluene added (1 L). The
head temperature was now 110.degree. C. A further 250 mL of solvent
was collected at 110.degree. C. Theoretically, this left the
product in ca. 2.4 L of toluene at 110.degree. C. This solution was
used in the next step.
[0234] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 7.83-7.80 (4H, m,
ArH), 7.63-7.51 (6H, m, ArH), 7.30-7.21 (10H, ArH), 3.89-3.80 (4H,
m, CH(a)+CH(b)), 3.73 (2H, s, CH.sub.2Ph(a)), 3.70 (2H, s,
CH.sub.2Ph(b)), 3.59 (2H, dd, CHHNSO.sub.2Ar(a)), 3.54 (2H, dd,
CHHNSO.sub.2Ar(b)), 3.40 (2H, dd, CHHNSO.sub.2Ar(b)), 3.23 (2H, dd,
CHHNSO.sub.2Ar(a)), 3.09-2.97 (4H, m, CHHNBn(a)+CHHNBn(b)), 2.83
(2H, dd, CHHNBn(b)), 2.71 (2H, dd, CHHNBn(a))
[0235] (Data taken from purified material comprising a 1:1 mixture
of trans- (a), and cis-diol (b))
(iii)
3-Benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane
[0236] The following is an alternative preparation to that
described in Preparation A(ii) above.
[0237] The toluene solution from the previous step (ii) above was
cooled to 50.degree. C. Anhydrous methanesulfonic acid (0.2 L) was
added. This caused a temperature rise from 50.degree. C. to
64.degree. C. After 10 minutes, methanesulfonic acid was added (1
L) and the reaction heated to 110.degree. C. for 5 hours. Toluene
was then distilled from the reaction; 1.23 L was collected. (Note
that the internal temperature should not be allowed higher than
110.degree. C. at any stage otherwise the yield will be decreased.)
The reaction was then cooled to 50.degree. C. and a vacuum applied
to remove the rest of the toluene. Heating to 110.degree. C. and
650 mbar allowed a further 0.53 L to be removed. (If the toluene
can be removed at a lower temperature and pressure then that is
beneficial.) The reaction was then left to cool to 30.degree. C.
and deionised water (250 mL) was added. This caused the temperature
to rise from 30.degree. C. to 45.degree. C. More water (2.15 L) was
added over a total time of 30 minutes such that the temperature was
less than 54.degree. C. The solution was cooled to 30.degree. C.
and then dichloromethane (2 L) was added. With external cooling and
rapid stirring, the reaction mixture was basified by adding aqueous
sodium hydroxide (10 M, 2 L) at a rate that kept the internal
temperature below 38.degree. C. This took 80 minutes. The stirring
was stopped and the phases separated in 3 minutes. The layers were
partitioned. IMS (2 L) was added to the dichloromethane solution
and distillation started. Solvent (2.44 L) was collected until the
head temperature reached 70.degree. C. Theoretically, this left the
product in 1.56 L of IMS. The solution was then allowed to cool to
ambient temperature overnight with slow stirring. The solid product
that precipitated was filtered and washed with IMS (0.5 L) to give
a fawn-coloured product that, on drying at 50.degree. C., in
vacuum, gave 50.8 g (8.9% over 3 steps). 20.0 g of this product was
dissolved in acetonitrile (100 mL) at reflux to give a pale yellow
solution. After cooling to ambient temperature, the crystals that
formed were collected by filtration and washed with acetonitrile
(100 mL). The product was dried in vacuo at 40.degree. C. for 1
hour to give 17.5 g (87%) of sub-title compound.
[0238] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 7.18-7.23 (10H,
m), 3.86-3.84 (2H, m), 3.67 (2H, d), 3.46 (2H, s), 2.91 (2H, d),
2.85 (2H, dd), 2.56 (2H, dd)
(iv) 3-Benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane.times.2 HCl
[0239] This is an alternative preparation to that described in
Preparation A(iii) above.
[0240] Concentrated hydrobromic acid (1.2 L, 3 rel. vol.) was added
to solid
3-benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane
(400 g, see step (iii) above) and the mixture was heated to reflux
under a nitrogen atmosphere. The solid dissolved in the acid at
95.degree. C. After heating the reaction for 8 hours, HPLC analysis
showed that the reaction was complete. The contents were cooled to
room temperature. Toluene (1.2 L, 3 rel. vol.) was added and the
mixture stirred vigorously for 15 minutes. Stirring was stopped and
the phases were partitioned. The toluene phase was discarded along
with a small amount of interfacial material. The acidic phase was
returned to the original reaction vessel and sodium hydroxide (10
M, 1.4 L, 3.5 rel. vol.) was added in one portion. The internal
temperature rose from 30.degree. C. to 80.degree. C. The pH was
checked to ensure it was >14. Toluene (1.6 L, 4 rel. vol.) was
added and the temperature fell from 80.degree. C. to 60.degree. C.
After vigorous stirring for 30 minutes, the phases were
partitioned. The aqueous layer was discarded along with a small
amount of interfacial material. The toluene phase was returned to
the original reaction vessel, and 2-propanol (4 L, 10 rel. vol.)
was added. The temperature was adjusted to between 40.degree. C.
and 45.degree. C. Concentrated hydrochloric acid (200 mL) was added
over 45 minutes such that the temperature remained at between
40.degree. C. and 45.degree. C. A white precipitate formed. The
mixture was stirred for 30 minutes and then cooled to 7.degree. C.
The product was collected by filtration, washed with 2-propanol
(0.8 L, 2 rel vol.), dried by suction and then further dried in a
vacuum oven at 40.degree. C. Yield=297 g (91%).
[0241] .sup.1H NMR (CD.sub.3OD+4 drops D.sub.2O): .delta. 2.70 (br
d, 2H), 3.09 (d, 2H), 3.47 (br s, 4H), 3.60 (s, 2H), 4.12 (br s,
2H), 7.30-7.45 (m, 5H).
[0242] API MS: m/z=219 [C.sub.13H.sub.18N.sub.2O+H].sup.+.
(v)
3,3-Dimethyl-1-[9-oxa-7-(phenylmethyl)-3,7-diazabicyclo[3.3.1]non-3-yl-
]-2-butanone
[0243] Water (500 mL, 5 vol.) followed by 1-chloropinacolone (45.8
mL, 1 eq.) were added to sodium bicarbonate (114.2 g, 4 eq.). A
solution of 3-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane.times.2
HCl (100.0 g; see step (iv) above) in water (300 mL, 3 vol.) was
added slowly, so that the evolution of carbon dioxide was
controlled (20 mins.). The reaction mixture was heated at 65 to
70.degree. C. for 4 hours. After cooling to ambient temperature,
dichloromethane (400 mL, 4 vol.) was added and, after stirring for
15 minutes, the phases were separated. The aqueous phase was washed
with dichloromethane (400 mL, 4 vol.) and the organic extracts
combined. The solution was distilled and solvent collected (550
mL). Ethanol (1 L) was added and the distillation continued.
Further solvent was collected (600 mL). Ethanol (1 L) was added and
the distillation continued. Further solvent was collected (500 mL)
(the head temperature was now 77.degree. C.). This solution
(theoretically containing 1150 mL of ethanol) was used directly in
the next step.
[0244] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 1.21 (9H, s),
2.01-2.59 (2H, m), 2.61-2.65 (2H, m), 2.87-2.98 (4H, m), 3.30 (2H,
s), 3.52 (2H, s), 3.87 (2H, br s), 7.26 (2H, d, J 7.6), 7.33 (1H,
dd, J 7.6, 7.6), 7.47 (2H, d, J 7.6).
(vi)
3,3-Dimethyl-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-2-butanone
[0245] Palladium on charcoal (44 g, 0.4 wt. eq. of 61% wet
catalyst, Johnson Matthey Type 440L) was added to the ethanol
solution from the previous step (v) above. The mixture was
hydrogenated at 4 bar. The reaction was considered complete after 5
hours. The catalyst was removed by filtration and washed with
ethanol (200 mL). The combined ethanol filtrates were used in
Example 3 below. Solution assay gave 61.8 g of title product in is
ethanol (theoretically 1.3-5 L; measured 1.65 L). A portion of the
product was isolated and purified. Analysis was performed on the
purified product.
[0246] .sup.1H NMR (300 MHz, CDCl.sub.3): .delta. 1.17 (9H, s),
2.69 (2H, dt, J 11.4, 2.4), 2.93 (2H, d, J 10.8), 3.02 (2H, d, J
13.8), 3.26 (2H, s), 3.32 (2H, dt, J 14.1), 3.61 (2H, br s).
Preparation O
2-(4-Acetyl-1-piperazinyl)ethyl 1H-imidazole-1-carboxylate
(i) 1-[4-(2-Hydroxyethyl)-1-piperazinyl]-1-ethanone
[0247] A solution of 2-(1-piperazinyl)-1-ethanol (6.5 g, 0.05 mol)
in DCM (5 mL) was treated with acetic acid anhydride (5.1 g, 0.05
mol), added dropwise. During addition, the reaction temperature
rose from 22 to 60.degree. C. The reaction mixture was evaporated
several times with toluene to yield 5.6 g (65%) of the sub-title
compound.
(ii) 2-(4-Acetyl-1-piperazinyl)ethyl 1H-imidazole-1-carboxylate
[0248] A solution of 1,1'-carbonyldiimidazole (5 g, 31 mmol) in DCM
(200 mL) was treated with a solution of
1-[4-(2-hydroxyethyl)-1-piperazinyl]-1-ethanone (from step (i)
above; 5 g, 29 mmol) in DCM (50 mL). The reaction mixture was
stirred at rt overnight before water was added. The layers were
separated, and the organic layer was washed with water, dried and
concentrated in vacuo to yield-7.4 g (96%) of the title
compound.
Preparation P
1-[4-(3-Bromopropyl)-1-piperazinyl]-1-ethanone
[0249] A mixture of 1-(1-piperazinyl)-1-ethanone (6.7 g, 0.052
mol), dibromopropane (330 mL, excess) and K.sub.2CO.sub.3 (10.2 g,
0.079 mol) was stirred at rt for 4 h. The mixture was washed with
4.times.100 mL of water, and the organic phase (diluted with DCM)
was acidified with aqueous hydrobromic acid (7 mL of 62% HBr
dissolved in 150 mL of water). The organic layer was separated and
washed with water (2.times.50 mL). The combined water layers were
extracted with ether, neutralised (to pH 7) with 13 mL of 10 M
NaOH, and then extracted with DCM. The combined organic layers were
dried and concentrated in vacuo to give 4.1 g (32%) of the title
compound.
Preparation Q
3-(Ethylsulfonyl)propyl 4-methylbenzenesulfonate
(i) 3-(Ethylsulfonyl)-1-propanol
[0250] A solution of 3-(ethylthio)-1-propanol (13 g, 0.11 mol) in
acetic acid (40 mL) was treated with H.sub.2O.sub.2 (30% in water,
12.2 g, 0.11 mol), added dropwise. The mixture was stirred for 2 h
at rt, before being concentrated in vacuo. NMR analysis showed that
the resulting residue consisted of 40% of the desired product and
60% of the corresponding O-acetate. The acetate was hydrolysed by
dissolving the reaction mixture in 200 mL of methanol and adding 3
g of NaOH (dissolved in a small amount of water). This mixture was
stirred overnight at rt, then concentrated in vacuo. The resulting
crude product was dissolved in DCM, and insoluble material was
filtered off. The DCM was removed by evaporation to give 13.4 g
(88%) is of the sub-title compound.
(ii) 3-(Ethylsulfonyl)propyl 4-methylbenzenesulfonate
[0251] A mixture of 3-(ethylsulfonyl)-1-propanol (from step (i)
above; 13.4 g, 88 mmol) and p-toluenesulfonyl chloride (16.8 g, 88
mmol) in DCM (150 mL) was treated with TEA (13.4 g, 132 mmol),
added dropwise. The resulting mixture was stirred at rt for 3 h
before being washed with aqueous ammonium chloride solution. The
organic layer was then separated, dried and concentrated in vacuo.
The product was crystallised from ether containing a small amount
of DCM to yield 17.9 g (66%) of the title compound.
Preparation R
tert-Butyl 2-bromoethylcarbamate
[0252] Sodium bicarbonate (6.15 g, 0.073 mol) and di-t-butyl
dicarbonate (11.18 g, 0.051 mol) were dissolved in a mixture of
H.sub.2O (50 mL) and dichloromethane (150 mL), then cooled to
0.degree. C. 2-Bromoethylamine hydrobromide (10.0 g, 0.049 mol) was
added slowly as a solid, and the reaction was stirred overnight at
25.degree. C. The dichloromethane layer was separated, washed with
H.sub.2O (200 mL) and washed with a solution of potassium
hydrogensulfate (150 mL, pH=3.5). The organic layer was dried
(Na.sub.2SO.sub.4) and concentrated in vacuo. The crude oil was
chromatographed on silica gel, eluting with dichloromethane to
afford 7.87 g (72%) of the sub-title compound as a clear, colorless
oil.
[0253] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 4.98 (bs, 1H),
3.45-3.57 (m, 4H), 1.47 (s, 9H)
[0254] API-MS: (M+1-C.sub.5H.sub.801) 126 m/z
Preparation S
2-(3,5-Dimethyl-1H-pyrazol-1-yl)ethyl 4-methylbenzenesulfonate
[0255] A cooled (0.degree. C.) mixture of
2-(3,5-dimethyl-1H-pyrazol-1-yl)-1-ethanol (0.48 g, 3.4 mmol) and
triethylamine (0.47 mL, 3.4 mmol) in MeCN (5 mL) was treated with
4-methylbenzenesulfonyl chloride (0.72 g, 3.8 mmol), after which
the mixture was kept cool in a refrigerator for 2 days. The mixture
was then concentrated in vacuo to give a residue which was purified
by chromatography on silica gel, eluting with ethyl acetate:hexane
(1:1), to give 0.46 g (46%) of the title compound.
Preparation T
4-(2-Bromoethoxy)phenyl tert-butyl ether
[0256] A solution of KOH (0.224 g, 4 mmol) in MeOH (3 mL) was
added, over the course of 30 min, to a warmed (70.degree. C.)
mixture of 1,2-dibromoethane (3 g, 0.016 mol) and
4-(tert-butoxy)phenol (0.66 g, 0.004 mol). The mixture was stirred
at 70.degree. C. for 15 h before water and CHCl.sub.3 were added.
The layers were separated, the organic layer washed with 10%
aqueous NaOH and then dried (Na.sub.2SO.sub.4) and concentrated in
vacuo to give 0.32 g (28%) of the sub-title compound.
Preparation U
3-Benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane via
Chirally-Enriched
5-Benzyl-3,7-dihydroxy-1-phenylsulfonyl-1,5-diazacyclooctane
(i) Chirally-Enriched
N,N-Bis(2-oxiranylmethyl)benzenesulfonamide
[0257] The following is an alternative procedure to that described
in Preparation M(i) above:
[0258] Water (100 mL, 10 vol) followed by (S)-epichlorohydrin (20
mL, 4 eq.) were added to benzenesulfonamide (10 g, 1 eq.). The
reactants were heated to 40.degree. C. Aqueous sodium hydroxide (10
M, 13 mL) was added over one hour, such that the temperature of the
reaction mixture remained between 37.degree. C. and 43.degree. C.
The reaction was then stirred at 40.degree. C. for 2 hours and at
ambient temperature overnight. The excess epichlorohydrin was
removed as a water azeotrope by vacuum distillation (ca. 30 mbar,
internal temp 30.degree. C.) until no more epichlorohydrin
distilled. Dichloromethane (200 mL) was added and the mixture was
stirred rapidly for 15 minutes. The mixture was then separated and
the dichloromethane layer was concentrated in vacuo to give a
colourless oil, which was used in the next step without further
purification.
[0259] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 7.51-7.87 (m,
5H), 3.65-3.54 (4H, m), 3.24-3.08 (4H, m), 2.82-2.77 (1H, m),
2.61-2.55 (1H, m)
(ii) Chirally-Enriched
5-Benzyl-3,7-dihydroxy-1-phenylsulfonyl-1,5-diazacyclooctane
[0260] The crude product from step (i) above was dissolved in
ethanol (200 mL) and treated at room temperature with benzylamine
(6.9 mL, 1 equiv.) in one portion (no exotherm was observed). The
mixture was heated to reflux for 4 hours, and was then stirred at
ambient temperature overnight. The solvent was removed in vacuo to
give a viscous, colourless oil which was used in the subsequent
step without further purification.
[0261] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 7.83-7.80 (2H, m,
ArH), 7.63-7.51 (3H, m, ArH), 7.30-7.21 (5H, ArH), 3.89-3.80 (2H,
m, CH), 3.73 (2H, s, CH.sub.2Ph), 3.59 (2H, dd, CHHNSO.sub.2Ar),
3.23 (2H, dd, CHHNSO.sub.2Ar), 3.09-2.97 (2H, m, CHHNBn), 2.71 (2H,
dd, CHHNBn).
(iii)
3-Benzyl-7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane
[0262] The crude product from step (ii) above was dissolved in warm
toluene (150 mL) and treated with anhydrous methanesulfonic acid
(50 mL). Toluene (105 mL) was removed from the mixture by
distillation at reduced pressure (28 mbar). The remaining mixture
was then heated to 110.degree. C. for 6.5 h. The mixture was
allowed to cool to 30.degree. C. and the remaining toluene removed
by distillation under reduced pressure (25 mbar). The mixture was
cooled in an ice/water bath to 40.degree. C. and then treated with
water (100 mL), which caused the internal temperature to rise to
70.degree. C. After cooling to 20-C dichloromethane (80 mL) was
added. The mixture was basified by the portionwise addition of
aqueous sodium hydroxide solution (10 M, 80 mL), such that the
internal temperature remained below 30.degree. C. This took 20
minutes. The dichloromethane layer was separated and evaporated
nearly to dryness in vacuo. Methanol (50 mL) was added and the
solvent was again removed in vacuo. The resulting solid was
suspended in MeOH (50 mL) and filtered. The filter cake was washed
with methanol (20 mL) and the resulting solid dried by air suction
to give the title compound as a white crystalline solid (3.46 g,
15% over 3 steps).
[0263] The following intermediates were either commercially
available or were prepared according to published methods: [0264]
ethyl isocyanate; [0265] 1-butanesulfonyl chloride; [0266]
1-chloropinacolone; [0267] 3,4-dimethoxyphenethyl methanesulfonate;
[0268] 1-(chloromethyl)cyclopropane; [0269]
2-bromo-1-(2,3-dihydro-1,4-benzodioxin-6-yl)-1-ethanone; [0270]
5-(2-chloroethyl)-4-methyl-1,3-thiazole; [0271]
2-chloro-N-isopropylacetamide; [0272]
1-bromo-2-(2-methoxyethoxy)ethane; [0273] 4-fluorobenzyl bromide;
[0274] 2-bromo-4'-methoxyacetophenone; [0275]
2-chloro-1-(4-fluorophenyl)-1-ethanone; [0276]
2-(bromomethyl)tetrahydro-2H-pyran; [0277]
1-bromo-3,3-dimethylbutane; [0278] chloroacetone; [0279]
N,N-diethylchloroacetamide; [0280]
4-chloro-1-(4-fluorophenyl)-1-butanone; [0281]
4-(bromomethyl)benzonitrile; [0282]
1-(bromomethyl)-2,4-difluorobenzene; [0283]
4-(difluoromethoxy)benzyl bromide; [0284] 1-(2-bromoethyl)pyrrole;
[0285] 1-(4-bromophenyl)-3-chloro-1-propanone; [0286]
2-bromo-1,1-difluoroethane; [0287] 1-(2-bromoethoxy)benzene; [0288]
2-(chloromethyl)imidazo[1,2-a]pyridine; [0289]
4-(2-chloroethyl)-1H-imidazole; [0290]
2-bromo-1-[4-(1-pyrrolidinyl)phenyl]-1-ethanone; [0291]
2-chloro-1-(4-hydroxyphenyl)-1-ethanone; [0292]
2-bromo-1-(4-methylphenyl)-1-ethanone; [0293]
2-bromo-1-(4-methoxyphenyl)-1-ethanone; [0294]
2-bromo-1-(2,3-dihydro-1,4-benzodioxin-6-yl)-1-ethanone; [0295]
6-(2-chloroacetyl)-2H-1,4-benzoxazin-3(4H)-one; [0296]
N-(tert-butyl)-N'-(2-chloroethyl)urea; [0297]
1-(chloromethyl)benzene; and [0298] tert-butyl
2-(bromomethyl)-1-pyrrolidinecarboxylate.
Synthesis of Compounds of Formula I
Example 1
4-{2-[7-(3,3-Dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]et-
hyl}benzonitrile
(i) tert-Butyl
7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-3-carbox-
ylate
[0299] A mixture of tert-butyl
9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate hydrochloride
(Preparation A; 0.26 g, 1.0 mmol) and K.sub.2CO.sub.3 (1.45 g, 10.5
mmol) in MeCN (8 mL) was treated with 1-chloropinacolone (0.216 g,
1.6 mmol), and the mixture stirred at 40.degree. C. overnight. The
following morning, the temperature was raised to 50.degree. C. for
4 h before the solids were filtered off from the mixture and the
filtrate concentrated in vacuo. The crude product was dissolved in
DCM and the solution was added to an ion-exchange solid phase
extraction plug (10 g CBA (carboxylic acid on silica support)).
After 1 h, the plug was washed with DCM (15 mL), after which the
product was finally eluted with dichloromethane:MeOH:TEA (90:5:5).
The solvents were evaporated to give 0.276 g (85.5%) of the
sub-title compound.
(ii)
3,3-Dimethyl-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-2-butanone
[0300] The following is an alternative preparation to that
described in Preparation N(v) above:
[0301] A solution of tert-butyl
7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxy-
late (from step (i) above; 0.265 g, 0.812 mmol) in ethyl acetate
(10 mL) was treated, at 0.degree. C., with ethyl acetate saturated
with gaseous hydrochloric acid. The mixture was stirred at
0.degree. C. for 5 h, and then evaporated. Acetonitrile (15 mL) and
K.sub.2CO.sub.3 (1 g, 7.2 mmol) were added, and the mixture was
stirred overnight before being filtered and evaporated to yield
0.159 g (86%) of the sub-title compound.
(iii) 4-Cyanophenethyl methanesulfonate
[0302] Methanesulfonyl chloride (18.6 g, 164 mmol) was added to a
stirred solution of 4-(2-hydroxyethyl)benzonitrile (20 g, 136 mmol)
and triethylamine (20.6 g, 204 mmol) in DCM (200 mL) at 0.degree.
C. The reaction mixture was stirred at rt until the reaction was
complete (as indicated by tlc). Water (200 mL) was added and the
organic layer was separated, dried and concentrated to give the
sub-title compound in a quantitative yield.
(iv)
4-{2-[7-(3,3-Dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3--
yl]ethyl}benzonitrile
[0303] A mixture of
3,3-dimethyl-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-2-butanone
(from step (ii) above; 56 mg, 0.25 mmol), TEA (0.35 mL, 2.5 mmol)
and MeCN (2 mL) was treated with 4-cyanophenethyl methanesulfonate
(see step (iii) above; 84 mg, 0.37 mmol). The resulting mixture was
stirred at 50.degree. C. for 24 h. The solvents were removed by
evaporation, the crude product was dissolved in DCM and then the
solution was added to an ion-exchange solid-phase extraction plug
(2 g, CBA (carboxylic acid on silica support)). After 1 h, the plug
was washed with DCM (15 mL), after which the product was finally
eluted with DCM:MeOH:TEA (90:5:5), to give 84 mg (95%) of the title
compound.
[0304] MS (ES): m/z=355.9 (M).sup.+.
Example 2
7-[4-(4-Cyanophenyl)-4-(3,4-dimethoxyphenoxy)butyl]-N-ethyl-9-oxa-3,7-diaz-
abicyclo[3.3.1]nonane-3-carboxamide
[0305] A solution of ethyl isocyanate (18.8 mg, 0.25 mmol) in MeCN
(2 mL) was added, together with K.sub.2CO.sub.3 (34.5 mg, 0.25
mmol), to a solution of
4-[1-(3,4-dimethoxyphenoxy)-4-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-but-
yl]benzonitrile (Preparation H, 109.4 mg, 0.25 mmol) in chloroform
(0.5 mL). The reaction mixture was stirred at rt for 4 days before
being added to a solid phase extraction plug (SiO.sub.2, 0.5 g).
The plug was washed with CHCl.sub.3:MeCN (2.5 mL of 80:20), and the
product was finally eluted with CHCl.sub.3:MeOH (3.times.2.5 mL of
95:5) to give the title compound.
[0306] MS (ES): m/z=508.3 (M).sup.+
Example 3
4-({3-[7-(3,3-Dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]p-
ropyl}amino)benzonitrile
Alternative A
[0307] A mixture of
4-{[3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]amino}-benzonitrile
(Preparation C; 5.73 g, 0.02 mol), K.sub.2CO.sub.3 (11.05 g, 0.08
mol) in MeCN (300 mL) was treated with 1-chloropinacolone (4.44 g,
0.032 mol). The mixture was stirred at 50.degree. C. overnight
before DCM was added and the mixture filtered. The filter cake was
then washed with a mixture of DCM and MeCN before the solvent was
evaporated from the filtrate. The resulting residue was purified by
chromatography on silica, eluting with a gradient of ethyl acetate
methanol:ammoniacal methanol (95:5:0 to 95:0:5), to give the title
compound (5.8 g, 73.9%).
Alternative B--Preparation via Benzenesulfonic Acid Salt
(i)
4-({3-[7-(3,3-Dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3--
yl]propyl}amino)benzonitrile, benzenesulfonic acid salt
monohydrate
[0308] Potassium carbonate (56.6 g, 1.5 equiv) and
3-(4-cyanoanilino)propyl-4-methylbenzenesulfonate (see Preparation
C(ii) above, 90.3 g, 1 equiv) were added to the ethanol solution of
3,3-dimethyl-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-2-butanone
(see Preparation N; 61.8 g from assay in 1.65 L). The reaction was
heated at 80.degree. C. for 4 hours. An assay showed some reactant
remained (8.3 g), so more
3-(4-cyanoanilino)propyl-4-methylbenzenesulfonate (12.2 g) was
added, and the resultant was heated at 80.degree. C. for 4 hours.
Solvent (1.35 L) was distilled, then isopropyl acetate (2.5 L)
added. Solvent (2.51 L) was removed. Isopropyl acetate (2.5 L) was
added. Solvent (0.725 L) was removed. The internal temperature was
now at 88.degree. C. Solvent (0.825 L) was removed, leaving the
product as an isopropylacetate solution (theoretically in 2.04 L).
After cooling to 34.degree. C., water (0.5 L) was added. There was
a black suspension, possibly of Pd, in the mixture. The pH of the
aqueous phase was 11. Sodium hydroxide (1 M, 0.31 L) was added, so
that the temperature was less than 25.degree. C., and the mixture
was stirred vigorously for 5 minutes. The pH of the aqueous phase
was 12. The phases were separated and the aqueous phase discarded.
More water (0.5 L) was added, and the phases were separated. The
aqueous phase was discarded. The remaining ester solution was
filtered to remove suspended particles, and the filtrate was then
made up to exactly 2 L. The solution was then split into 2.times.1
L portions.
[0309] (In order to avoid producing sub-title product comprising a
high palladium content, the following treatment may be performed:
Deloxan.RTM. resin (12.5 g, 25 wt %) was added to the solution of
the free base (1 L), and the mixture heated at reflux with vigorous
stirring for 5 hours. The solution was then cooled to room
temperature, and was stirred for 2 days. The resin was removed by
filtration.)
[0310] An assay was performed to calculate the required amount of
benzenesulfonic acid, to make the benzenesulfonate salt.
[0311] A solution of benzenesulfonic acid (20.04 g, 1 eq., assuming
acid was pure monohydrate) in isopropyl acetate (200 mL) was added
over 5 minutes (better to add slower if possible) with vigorous
stirring to the solution of the free base (1 L) and a pale yellow
precipitate formed. The temperature rose from 18.degree. C. to
22.degree. C. After 10 minutes, the mixture was cooled to
10.degree. C. and the product collected by filtration. The product
was washed with isopropyl acetate (250 mL), sucked dry on the
filter then dried under vacuum at 40.degree. C. for 2 days to give
59.0 g (61% from
3-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]nonane.times.2 HCl).
[0312] (The crude benzenesulfonate salt was alternatively prepared
by the addition of a 70% (w/w) aqueous solution of benzenesulfonic
acid to an ethanolic solution of the free base.)
[0313] The crude sub-title product is isolated as a
monohydrate.
[0314] Ethanol (500 mL) and water (250 mL) were added to crude
sub-title compound (50.0 g). The solution was heated to 75.degree.
C. Material was all dissolved at 55.degree. C. The solution was
held at 75.degree. C. for 5 minutes, then cooled to 5.degree. C.
over 1 hour. Precipitation started at 18.degree. C. The cold
solution was filtered and the filtrate washed with ethanol:water
(2:1; 150 mL), sucked dry on the filter, and then dried in vacuo at
40.degree. C. to give pure sub-title product (41.2 g, 82%).
[0315] (This recrystallisation may be carried out with greater
volumes of solvent if necessary to fit the reaction vessels
e.g.
EtOH:water 2:1, 45 vol. (gave 62% recovery) EtOH:water 6:1, 35 vol.
(gave 70% recovery).)
[0316] The sub-title product was isolated as the monohydrate
following the rescrystallisation (as determined by single crystal
X-ray diffraction).
(ii)
4-({3-[7-(3,3-Dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-
-yl]propyl}amino)benzonitrile
[0317] Crude benzenesulfonate salt (50.0 g, 1.0 equiv, from step
(i) above) was added to aqueous sodium hydroxide (1M, 500 mL)
washing in with dichloromethane (1.0 L, 20 vol). The combined
mixture was stirred for 15 minutes. The layers were then separated
and a small amount of interfacial material was left with the upper
aqueous layer. Ethanol (500 mL, 10 vol) was added to the
dichloromethane solution and then solvent was removed by
distillation (1.25 L). The still head temperature was now at
78.degree. C. The solution was allowed to cool to below reflux and
ethanol (250 mL, 5 vol.) was added. Solvent was removed (250 mL).
This warm solution was diluted with ethanol to 890 mL, 17.8 vol.
(25 vol. assuming 100% conversion to free base). After heating to
reflux the solution was cooled slowly. At 5.degree. C. a seed of
title compound was added. Crystallisation began and the mixture was
stirred at 5.degree. C. for 30 minutes. The product was collected
by filtration and washed with ethanol (2.times.50 mL, 2.times.1
vol.). The product was then dried in a vacuum oven at 40.degree. C.
for 60 hours to give an off-white powder (26.3 g; 74%).
[0318] .sup.1H NMR (400 MHz, CDCl.sub.3): .delta. 7.86-7.82 (2H,
m), 7.39-7.32 (3H, m), 7.30-7.26 (2H, m), 6.47 (2H, m), 4.11-4.07
(4H, m), 3.70 (2H, s), 3.36-3.33 (4H, m), 3.26 (2H, t), 3.12 (2H,
d), 2.90 (2H, d), 2.28-2.21 (2H, m), 1.06 (9H, s).
[0319] .sup.13C NMR (CDCl.sub.3): .delta. 24.07, 26.38, 41.52,
43.52, 56.17, 56.47, 63.17, 68.46, 96.61, 111.64, 121.03,
133.43.
[0320] MS (ES): m/z=385.1 (M+H).sup.+
Example 4
4-{3-[7-(4-Fluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-2-hydroxyp-
ropoxy}benzonitrile
[0321] A solution of 4-fluorobenzyl bromide (14.17 mg, 0.075 mmol)
in DCM (0.5 mL) of was added, together with TEA (20 mg, 0.2 mmol)
to a solution of
4-{[2-hydroxy-3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-propyl]oxy}ben-
zonitrile (Preparation K; 15.2 mg, 0.05 mmol) in MeCN (0.5 mL). The
mixture was stirred at 50.degree. C. for 4 days before being
concentrated in vacuo. The resulting residue was dissolved in
CHCl.sub.3 and added to a solid-phase extraction plug (CBA, 0.4 g).
The plug was washed with CHCl.sub.3 (4.times.0.3 mL), and the
product was finally eluted with CHCl.sub.3:MeOH:TEA (5.times.0.3 mL
of 8:1:1) to give the title compound.
[0322] MS (ES): m/z=412.5 (M+H).sup.+.
Example 5
4-(2-{7-[2-(4-Methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-
-3-yl}ethoxy)benzonitrile
[0323] A mixture of
4-[2-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]benzonitrile
(Preparation D; 68.3 mg, 0.25 mmol), 2-bromo-4'-methoxyacetophenone
(68.7 mg, 0.3 mmol) and TEA (37.94 mg, 0.37 mmol) in DMF (2.5 mL)
was stirred at rt for 2 days, and then (as reaction was found to be
incomplete) at 50.degree. C. for 24 h. The solvent was evaporated
and the residue dissolved in a mixture of MeCN (2.5 mL) and
H.sub.2O (0.13 mL). Potassium carbonate (100 mg, 0.72 mmol) was
added, and the mixture was stirred overnight at rt. The mixture was
then filtered and the filtrate concentrated in vacuo. The resulting
crude product was dissolved in DCM (2 mL), which solution was added
to an ion-exchange solid-phase extraction plug (CBA, 2 g). After 80
min, the product was eluted with DCM:MeCN (4:1) and then with
DCM:MeOH:TEA (8:1:1), to give an impure material. This material was
purified on a silica plug, eluting with CHCl.sub.3 (2 mL),
CHCl.sub.3:CH.sub.3CN (3.times.2.5 mL of 4:1), and then with
CHCl.sub.3:MeOH (10:1), to give 71.5 mg (67.9%) of the title
compound.
[0324] MS (ES): m/z=422.4 (M+H).sup.+.
[0325] .sup.13C NMR (CDCl.sub.3): .delta. 55.47, 55.89, 56.27,
57.17, 66.57, 66.81, 67.41, 102.99, 113.57, 115.73, 119.29, 131.56,
134.32, 162.09, 163.21, 196.25.
Example 6
4-[((2S)-2-Amino-3-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo-[3.-
3.1]non-3-yl}propyl)oxy]benzonitrile
[0326] A mixture of tert-butyl
(1S)-2-(4-cyanophenoxy)-1-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-ylmethyl)et-
hylcarbamate (Preparation J; 100.62 mg, 0.25 mmol),
1-(2-bromoethyl)pyrrole (52.21 mg, 0.30 mmol) and TEA (37.9 mg,
0.375 mmol) in DMF (2.5 mL) was stirred at rt for 2 days and then
at 50.degree. C. for 1 day. The solvent was evaporated and the
resulting residue dissolved in ethyl acetate (0.5 mL). Ethyl
acetate saturated with gaseous hydrochloric acid (2 mL) was added,
and the reaction mixture stirred for 1.5 h at rt. The solvent was
evaporated and the resulting residue dissolved in a mixture of MeCN
(2.5 mL) and H.sub.2O (0.13 mL). Potassium carbonate (100 mg, 0.72
mmol) was added, and the mixture stirred overnight at rt. The
mixture was filtered and the filtrate concentrated in vacuo. The
resulting crude product was dissolved in DCM (2 mL), which solution
was added to a ion-exchange solid-phase extraction plug (CBA, 2 g).
After 80 min, the product was eluted with DCM:MeCN (4.times.2 mL of
4:1), followed by DCM:MeOH:TEA (8:1:1), to give 89.7 mg (90.7%) of
the title compound.
[0327] MS (ES): m/z=396.0 (M).sup.+
Example 7
tert-Butyl
2-{7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]-n-
on-3-yl}ethylcarbamate
[0328] To a solution of tert-butyl 2-bromoethylcarbamate (4.21 g,
0.019 mol; see Preparation R above) in DMF (65 mL) was added
4-{[3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]amino}benzonitrile
(see Preparation C above, 4.48 g, 0.016 mol) and triethylamine
(3.27 mL, 0.024 mol). The mixture was stirred overnight at
35.degree. C. and then concentrated in vacuo. The residue was
dissolved in dichloromethane (80 mL) and washed with saturated
sodium chloride. The aqueous layer was extracted with
dichloromethane (1.times.150 mL). The combined organic extracts
were dried (Na.sub.2SO.sub.4) and concentrated in vacuo. The crude
red-brown oil was chromatographed (.times.2) on silica gel eluting
with chloroform:methanol:conc. NH.sub.4OH (9:1:0.02) to afford 3.75
g (56%) of the title compound.
[0329] .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.37-7.40 (d,
J=8.8 Hz, 2H), 6.64-6.67 (d, J=8.8 Hz, 2H), 3.94 (bs, 2H),
3.21-3.31 (m, 4H), 3.01 (bs, 4H), 2.47-2.59 (m, 8H), 1.90 (bs, 2H),
1.39 (s, 9H)
[0330] .sup.13C NMR (75 MHz, CD.sub.3OD) .delta. 158.5, 134.7,
121.9, 113.2, 97.7, 80.3, 69.2, 58.8, 58.1, 57.5, 57.3, 41.9, 38.3,
28.9, 26.2.
[0331] API-MS: (M+1)=430 m/z
Example 8
tert-Butyl
2-{7-[4-(4-cyanophenyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]non--
3-yl}ethylcarbamate
[0332] Triethylamine (2.2 mL, 0.016 mol) and tert-butyl
2-bromoethylcarbamate (see Preparation R above, 2.83 g, 0.013 mol)
were added to a solution of
4-[4-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)butyl]benzonitrile (see
Preparation G above, 3.0 g, 0.011 mol) in DMF (50 mL). The mixture
was stirred for 24 h at 54.degree. C., cooled to 25.degree. C., and
concentrated in vacuo. The residue was dissolved in chloroform and
washed with saturated sodium chloride. The aqueous layer was
separated and extracted with chloroform (2.times.150 mL). The
combined organic layers were dried (Na.sub.2SO.sub.4) and
concentrated in vacuo. The resulting material was chromatographed
on silica gel, eluting first with chloroform:acetonitrile:conc.
ammonium hydroxide (9:1:0.02) until the higher R.sub.f impurities
were removed. Then the eluent was switched to
chloroform:methanol:conc. ammonium hydroxide (9:1:0.02). This
afforded 2.76 g (61%) of the title compound.
[0333] .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.62-7.64 (d,
J=8.1 Hz, 2H), 7.38-7.40 (d, J=8.1 Hz, 2H), 3.84 (s, 2H), 3.14-3.18
(t, J=6.0 Hz, 2H), 2.83-2.93 (m, 4H), 2.72-2.77 (t, J=6.9 Hz, 2H),
2.30-2.50 (m, 8H), 1.64-1.68 (m, 4H), 1.43 (s, 9H)
[0334] .sup.13C NMR (75 MHz, CD.sub.3OD) .delta. 158.4, 149.6,
133.4, 130.7, 120.1, 110.7, 79.9, 69.8, 61.1, 58.7, 57.7, 57.1,
38.0, 36.9, 30.2, 29.0, 26.7
Example 9
tert-Butyl
2-[7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazab-
icyclo[3.3.1]non-3-yl]ethylcarbamate
[0335] Triethylamine (8.56 mL, 0.061 mol) and tert-butyl
2-bromoethylcarbamate (see Preparation R above, 11.0 g, 0.049 mol)
were added to a solution of
4-{[(2S)-2-hydroxy-3-(9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)-propyl]oxy}b-
enzonitrile (see preparation B above, 12.41 g, 0.038 mol) in DMF
(100 mL). The mixture was stirred for 20 h at 40.degree. C., then
concentrated in vacuo. The residue was dissolved in chloroform (100
mL), and washed with saturated sodium chloride. The aqueous layer
was separated and extracted with chloroform (2.times.150 mL). The
combined organic layers were dried (Na.sub.2SO.sub.4) and
concentrated in vacuo. The crude brown oil was chromatographed
(.times.2) on silica gel, eluting first with chloroform:methanol
(9:1), then with chloroform:methanol:conc. ammonium hydroxide
(9:1:0.05) to afford 4.13 g (24%) of the title compound.
[0336] .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.62-7.69 (d,
J=8.0 Hz, 2H), 7.09-7.14 (d, J=8.0 Hz, 2H), 4.00-4.17 (m, 3H), 3.87
(s, 2H), 3.18-3.24 (m, 2H), 2.88-3.03 (m, 4H), 2.65-2.70 (m, 2H),
2.47-2.55 (m, 4H), 2.31-2.39 (m, 2H), 1.41 (s, 9H)
[0337] .sup.13C NMR (75 MHz, CD.sub.3OD) .delta. 163.9., 158.3,
135.3, 120.2, 116.7, 104.9, 80.0, 72.1, 70.1, 69.9, 67.0, 60.6,
60.2, 58.4, 57.8, 55.7, 38.31, 28.99.
[0338] API-MS: (M+1)=447 m/z
Example 10
4-(2-{7-[4-(4-Pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-etho-
xy)benzonitrile
(i) 4-(4-Chlorobutyl)pyridine
[0339] 4-Methylpyridine (8.4 g, 90 mmol) and THF (40 mL) were mixed
in dry glassware, flushed with nitrogen and cooled to -60.degree.
C. n-BuLi (1.6 M solution, 61.9 mL, 99 mmol) was added dropwise
over 1.5 h. The temperature was not allowed to exceed -50.degree.
C. The mixture was then allowed to reach rt, THF (20 mL) was added
and the mixture was then stirred at 45.degree. C. for 2 h.
Additional THF (20 mL) was added. This mixture was cooled to
0.degree. C. and added dropwise through a cooled dropping funnel to
a 65.degree. C. solution of 3-bromo-1-chloropropane (14.9 g, 94.5
mmol) in THF (15 mL). The reaction mixture was slowly allowed to
reach 0.degree. C. overnight. Water (90 mL) was added, and the
mixture was stirred for 10 min. The organic layer was separated and
dried (Na.sub.2SO.sub.4) to give the sub-title compound in 97.6%
yield.
(ii)
4-(2-{7-[4-(4-Pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
-ethoxy)benzonitrile
[0340]
4-[2-(9-Oxa-3,7-diazabicyclo[3.3.1]non-3-yl)ethoxy]benzonitrile
(0.80 g, 2.92 mmol, see preparation D above) was dissolved in MeCN
(30 mL) and mixed with 4-(4-chlorobutyl)pyridine (0.74 g, 4.39
mmol, from step (i) above) and K.sub.2CO.sub.3 (1.62 g, 11.71
mmol). 1 drop of Br.sub.2 was added and the mixture was refluxed
for 24 h. The mixture was filtered and evaporated. Purification by
chromatography on silica, eluting with DCM:4% MeOH (satd. with
ammonia), gave 0.68 g (57.2%) of the title compound.
[0341] .sup.13C NMR (CDCl.sub.3) .delta. 25.78, 27.82, 34.92,
56.28, 56.50, 57.63, 58.99, 66.57, 68.11, 103.94, 115.20, 119.07,
123.75, 133.88, 149.57, 151.35, 161.91
Example 11
tert-Butyl
2-{7-[4-(4-pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3--
yl}ethylcarbamate
(i) tert-Butyl
7-[4-(4-pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-3-carboxyla-
te
[0342] tert-Butyl 9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylate
(1.35 g, 5.9 mmol, see Preparation A and Preparation C(iii) above)
was mixed with 4-(4-chlorobutyl)pyridine (1.35 g, 7.37 mmol, see
Example 10(i) above), Br.sub.2 (0.094 g, 0.59 mmol) and
K.sub.2CO.sub.3 (3.26 g, 23.6 mmol). The mixture was refluxed under
argon for 3 days. The reaction mixture was filtered, evaporated and
purified by chromatography (DCM, 2-5% MeOH) giving 0.97 g (44%) of
the sub-title compound.
[0343] .sup.13C NMR (CDCl.sub.3) .delta. 25.83, 27.78, 28.48,
35.09, 45.80, 47.16, 56.57, 57.42, 58.99, 67.47, 67.72, 79.00,
123.88, 149.60, 151.26, 154.49
(ii)
3-[4-(4-Pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane
[0344] tert-Butyl
7-[4-(4-pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxylat-
e (0.9 g, 2.5 mmol, from step (i) above) was dissolved in
ethylacetate, and then treated with ethylacetate saturated with HCl
at 0.degree. C. The mixture was stirred for 1 h at 0.degree. C.,
and then at rt overnight. The solvent was evaporated. CH.sub.3CN
(100 mL) and water (2 mL) were added together with K.sub.2CO.sub.3
(3.22 g). The mixture was stirred overnight. Filtration and
evaporation gave the sub-title compound in 94% yield.
(iii) tert-Butyl
2-{7-[4-(4-pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-3-yl}ethylc-
arbamate
[0345] 3-[4-(4-Pyridinyl)butyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane
(0.25 g, 0.96 mmol, from step (ii) above), tert-butyl
2-bromoethylcarbamate (0.26 g, 0.98 mmol, see Preparation R above)
and K.sub.2CO.sub.3 (0.4 g, 2.9 mmol) were mixed in CH.sub.3CN (10
mL) and stirred at 50.degree. C. overnight. The reaction mixture
was filtered, evaporated and purified by chromatography on silica
(DCM:6% MeOH (satd. with NH.sub.3)). Additional purification by
extraction with ether:KHSO.sub.4, basification of the organic phase
and extraction with DCM gave the title compound in 51% yield.
[0346] .sup.13C NMR (CD.sub.3OD) .delta. 26.65, 28.86, 29.38,
36.02, 38.00, 56.93, 57.58, 58.47, 60.93, 69.85, 79.93, 125.65,
149.85, 154.43, 158.40
Example 12
[0347] The following compounds were prepared, from appropriate
intermediates (such as those described hereinbefore), according to
or by analogy with methods described herein and/or by standard
solid or solution phase combinatorial chemistry techniques (mass
spectra of the compounds, where recorded, are in brackets): [0348]
4-{3-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]--
2-hydroxypropoxy}benzonitrile (m/z=402.5); [0349]
4-{3-[7-(3,4-dimethoxyphenethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-2-
-hydroxypropoxy}benzonitrile (m/z=467.2); [0350]
4-{2-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]--
ethoxy}benzonitrile (m/z=371.2); [0351]
4-({3-[7-(butylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propyl}-am-
ino)benzonitrile (m/=406.2); [0352]
4-({3-[7-(3,4-dimethoxyphenethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]p-
ropyl}amino)benzonitrile (m/z=450.3); [0353]
4-[4-[7-(butylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-1-(3,4-dim-
ethoxyphenoxy)butyl]benzonitrile (m/z=557.3); [0354]
4-{1-(3,4-dimethoxyphenoxy)-4-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diaz-
abicyclo[3.3.1]non-3-yl]butyl}benzonitrile (m/z=535.3); [0355]
4-[4-[7-(3,4-dimethoxyphenethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-1-
-(3,4-dimethoxyphenoxy)butyl]benzonitrile (m/z=601.3); [0356]
2-(4-acetyl-1-piperazinyl)ethyl
7-[3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonan-
e-3-carboxylate (m/z=501.3); [0357]
7-[3-(4-cyanophenoxy)-2-hydroxypropyl]-N-ethyl-9-oxa-3,7-diazabicyclo-[3.-
3.1]nonane-3-carboxamide (m/z=374.2); [0358]
4-{3-[7-(butylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-2-hydroxyp-
ropoxy}benzonitrile (m/z=423.4); [0359]
2-(4-acetyl-1-piperazinyl)ethyl
7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carboxy-
late (m/z=471.2); [0360]
7-[2-(4-cyanophenoxy)ethyl]-N-ethyl-9-oxa-3,7-diazabicyclo[3.3.1]-nonane--
3-carboxamide (m/z=344.2); [0361]
4-{2-[7-(butylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]ethoxy}-ben-
zonitrile (m/z=393.2); [0362]
4-{2-[7-(3,4-dimethoxyphenethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]et-
hoxy}benzonitrile (m/z=437.2); [0363]
2-(4-acetyl-1-piperazinyl)ethyl
7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]nonane-3-carbox-
ylate (m/z=484.3); [0364]
7-[3-(4-cyanoanilino)propyl]-N-ethyl-9-oxa-3,7-diazabicyclo[3.3.1]-nonane-
-3-carboxamide (m/z=357.2); [0365] 2-(4-acetyl-1-piperazinyl)ethyl
7-[4-(4-cyanophenyl)-4-(3,4-dimethoxyphenoxy)butyl]-9-oxa-3,7-diazabicycl-
o[3.3.1]nonane-3-carboxylate (m/z=635.3); [0366]
4-{3-[7-(cyclopropylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-2-hydr-
oxypropoxy}benzonitrile (m/z=358.5); [0367]
4-(3-{7-[2-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-oxoethyl]-9-oxa-3,7-diaza-
bicyclo[3.3.1]non-3-yl}-2-hydroxypropoxy)benzonitrile (m/z=480.5);
[0368]
4-(3-{7-[3-(4-acetyl-1-piperazinyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]--
non-3-yl}-2-hydroxypropoxy)benzonitrile (m/z=472.5); [0369]
2-{7-[3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabicyclo-[3.3.1]n-
on-3-yl}-N-isopropylacetamide (m/z=403.5); [0370]
4-(3-{7-[3-(ethylsulfonyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
2-hydroxypropoxy)benzonitrile (m/z=438.5); [0371]
4-(2-hydroxy-3-{7-[2-(2-methoxyethoxy)ethyl]-9-oxa-3,7-diazabicyclo-[3.3.-
1]non-3-yl}propoxy)benzonitrile (m/z=406.5); [0372]
4-(2-hydroxy-3-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo-
[3.3.1]non-3-yl}propoxy)benzonitrile (m/z=452.5); [0373]
4-({3-[7-(cyclopropylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-propy-
l}amino)benzonitrile (m/z=341.5); [0374]
4-[(3-{7-[2-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-oxoethyl]-9-oxa-3,7-diaz-
abicyclo[3.3.1]non-3-yl}propyl)amino]benzonitrile (m/z=463.5);
[0375]
4-[(3-{7-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-9-oxa-3,7-diazabicyclo-[3.3-
.1]non-3-yl}propyl)amino]benzonitrile (m/z=412.5); [0376]
4-[(3-{7-[3-(4-acetyl-1-piperazinyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
-non-3-yl}propyl)amino]benzonitrile (m/z=455.6); [0377]
2-{7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-N--
isopropylacetamide (m/z=386.5); [0378]
4-[(3-{7-[3-(ethylsulfonyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
-propyl)amino]benzonitrile (m/z=421.5); [0379]
4-[(3-{7-[2-(2-methoxyethoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl-
}propyl)amino]benzonitrile (m/z=389.5); [0380]
4-({3-[7-(4-fluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propyl}-a-
mino)benzonitrile (m/z=395.5); [0381]
4-[(3-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-n-
on-3-yl}propyl)amino]benzonitrile (m/z=435.5); [0382]
4-{2-[7-(cyclopropylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-ethoxy-
}benzonitrile (m/z=328.4); [0383]
4-(2-{7-[2-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-oxoethyl]-9-oxa-3,7-diaza-
bicyclo[3.3.1]non-3-yl}ethoxy)benzonitrile (m/z=450.5); [0384]
4-(2-{7-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1-
]non-3-yl}ethoxy)benzonitrile (m/z=399.5); [0385]
4-(2-{7-[3-(4-acetyl-1-piperazinyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]--
non-3-yl}ethoxy)benzonitrile (m/z=442.5); [0386]
2-{7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-N-i-
sopropylacetamide (m/z=373.5); [0387]
4-(2-{7-[3-(ethylsulfonyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
ethoxy)benzonitrile (m/z=408.5); [0388]
4-(2-{7-[2-(2-methoxyethoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
ethoxy)benzonitrile (m/z=376.5); [0389]
4-{2-[7-(4-fluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]ethoxy}-be-
nzonitrile (m/z=382.5); [0390]
4-({3-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-
propyl}sulfonyl)benzonitrile (m/z=434.5); [0391]
4-({3-[7-(cyclopropylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-propy-
l}sulfonyl)benzonitrile (ml/=390.5); [0392]
4-[(3-{7-[2-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-oxoethyl]-9-oxa-3,7-diaz-
abicyclo[3.3.1]non-3-yl}propyl)sulfonyl]benzonitrile (m/z=512.4);
[0393]
4-[(3-{7-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-9-oxa-3,7-diazabicyclo-[3.3-
.1]non-3-yl}propyl)sulfonyl]benzonitrile (m/z=461.4); [0394]
4-[(3-{7-[3-(4-acetyl-1-piperazinyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]-
-non-3-yl}propyl)sulfonyl]benzonitrile (m/z=504.5); [0395]
2-(7-{3-[(4-cyanophenyl)sulfonyl]propyl}-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl)-N-isopropylacetamide (m/z=435.5); [0396]
4-[(3-{7-[3-(ethylsulfonyl)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
-propyl)sulfonyl]benzonitrile (m/z=470.4); [0397]
4-[(3-{7-[2-(2-methoxyethoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl-
}propyl)sulfonyl]benzonitrile (m/z=438.5); [0398]
4-({3-[7-(4-fluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propyl}-s-
ulfonyl)benzonitrile (m/z=444.4); [0399]
4-[(3-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-n-
on-3-yl}propyl)sulfonyl]benzonitrile (m/z=484.4); [0400]
4-[(3-{7-[2-(4-fluorophenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl}propyl)amino]benzonitrile (m/z=423.4); [0401]
4-(2-{7-[2-(4-fluorophenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non--
3-yl}ethoxy)benzonitrile (m/z=410.4); [0402]
4-{2-[7-(tetrahydro-2H-pyran-2-ylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl]ethoxy}benzonitrile (m/z=372.4); [0403]
4-(3-{7-[2-(4-fluorophenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non--
3-yl}-2-hydroxypropoxy)benzonitrile (m/z=440.4); [0404]
4-{2-hydroxy-3-[7-(tetrahydro-2H-pyran-2-ylmethyl)-9-oxa-3,7-diazabicyclo-
[3.3.1]non-3-yl]propoxy}benzonitrile (m/=402.4); [0405]
4-({3-[7-(2-fluoro-3,3-dimethylbutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3--
yl]propyl}amino)benzonitrile (m/z=389.3); [0406]
4-({3-[7-(2-hydroxy-3,3-dimethylbutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-
-yl]propyl}amino)benzonitrile (m/z=387.0); [0407]
4-({3-[7-(3,3-dimethylbutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-propy-
l}amino)benzonitrile (m/z=371.01); [0408]
4-({3-[7-(2-oxopropyl)-9-oxa-3,7-diazabicyclo[3).3.1]non-3-yl]propyl}-ami-
no)benzonitrile (m/z=342.92); [0409]
4-(2-{7-[--(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
ethoxy)benzonitrile (m/z=431.9); [0410]
4-(2-{7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}e-
thoxy)benzonitrile (m/z=418.9); [0411]
4-(2-{7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}e-
thyl)benzonitrile (m/z=402.9); [0412]
4-{4-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]--
butyl}benzonitrile (m/z=383.9); [0413]
4-{2-[7-(2-phenoxyethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]ethoxy}-be-
nzonitrile (m/z=393.9); [0414]
2-{7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-N,N-
-diethylacetamide (m/z=387.0); [0415]
4-[(3-{7-[4-(4-fluorophenyl)-4-oxobutyl]-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl}propyl)amino]benzonitrile (m/z=450.9); [0416]
4-({7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-m-
ethyl)benzonitrile (m/z=401.9); [0417]
4-{2-[7-(2,4-difluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-ethox-
y}benzonitrile (m/z=400.0); [0418]
4-[(3-{7-[4-(difluoromethoxy)benzyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-y-
l}propyl)amino]benzonitrile (m/z, =442.9); [0419]
4-[(3-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}-
-propyl)amino]benzonitrile (m/z=379.9); [0420]
4-[(3-{7-[3-(4-bromophenyl)-3-oxopropyl]-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl}propyl)amino]benzonitrile (m/z=496.8); [0421]
4-{2-[7-(2,2-difluoroethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]ethoxy}-
-benzonitrile (m/z=337.8); [0422]
4-({3-[7-(2-phenoxyethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propyl}-a-
mino)benzonitrile (m/z=407.4); [0423]
4-(2-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
ethoxy)benzonitrile (m/z=367.4); [0424]
4-[((2S)-3-{7-[(2S)-3-(4-cyanophenoxy)-2-hydroxypropyl]-9-oxa-3,7-diazabi-
cyclo[3.3.1]non-3-yl}-2-hydroxypropyl)oxy]benzonitrile; [0425]
4-[((2S)-2-hydroxy-3-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo--
[3.3.1]non-3-yl}propyl)oxy]benzonitrile (m/z=397.4); [0426]
4-{2-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]--
ethoxy}isophthalonitrile (m/z=397.4); [0427]
4-(2-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl}ethoxy)isophthalonitrile (m/z=447.4); [0428]
4-(2-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
ethoxy)isophthalonitrile (m/z=392.4); [0429] tert-butyl
2-{7-[2-(2,4-dicyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo-[3.3.1]non-3-yl-
}ethylcarbamate (m/z=442.4); [0430]
4-({(2S)-2-amino-3-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo-[3-
.3.1]non-3-yl]propyl}oxy)benzonitrile (m/z=401.0); [0431]
4-[((2S)-2-amino-3-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabic-
yclo[3.3.1]non-3-yl}propyl)oxy]benzonitrile (m/z=451.0); [0432]
4-{3-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]--
propoxy}benzonitrile (m/z=386.4); [0433]
4-(3-{7-[2-(4-fluorophenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non--
3-yl}propoxy)benzonitrile (m/z=424.4); [0434]
4-(3-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
propoxy)benzonitrile (m/z=381.4); [0435]
4-(4-{7-[2-(1H-pyrrol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}--
butyl)benzonitrile (m/z=379.4); [0436]
4-{[(2S)-3-(7-{2-[4-(tert-butoxy)phenoxy]ethyl}-9-oxa-3,7-diazabicyclo-[3-
.3.1]non-3-yl)-2-hydroxypropyl]oxy}benzonitrile (m/z=496.6); [0437]
4-[((2S)-3-{7-[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]-9-oxa-3,7-diazabicy-
clo[3.3.1]non-3-yl}-2-hydroxypropyl)oxy]benzonitrile (m/z=426.5);
4-{3-[7-(imidazo[1,2-a]pyridin-2-ylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]n-
on-3-yl]propoxy}benzonitrile (m/z=418.5); [0438]
4-{3-[7-(2-phenoxyethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propoxy}-b-
enzonitrile (m/z=408.5); [0439]
4-(3-{7-[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.-
3.1]non-3-yl}propoxy)benzonitrile (m/z=410.5); [0440]
4-({3-[7-(imidazo[1,2-a]pyridin-2-ylmethyl)-9-oxa-3,7-diazabicyclo-[3.3.1-
]non-3-yl]propyl}amino)benzonitrile (m/z=417.5); [0441]
4-({3-[7-(2,4-difluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-prop-
yl}amino)benzonitrile (m/z=413.5); [0442]
4-{[3-(7-{2-[4-(tert-butoxy)phenoxy]ethyl}-9-oxa-3,7-diazabicyclo[3.3.1]--
non-3-yl)propyl]amino}benzonitrile (m/z=479.6); [0443]
4-{2-[7-(imidazo[1,2-a]pyridin-2-ylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]--
non-3-yl]ethoxy}benzonitrile (m/z=404.5); [0444] tert-butyl
2-{7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-3-yl}eth-
ylcarbamate (m/z=417.5); [0445]
4-{[3-(7-{2-[4-(tert-butoxy)phenoxy]ethyl}-9-oxa-3,7-diazabicyclo[3.3.1]n-
on-3-yl)propyl]sulfonyl}benzonitrile (m/z=528.5); [0446]
4-[(3-{7-[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo-[-
3.3.1]non-3-yl}propyl)sulfonyl]benzonitrile (m/z=458.5); [0447]
4-({3-[7-(2,4-difluorobenzyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-prop-
yl}sulfonyl)benzonitrile (m/z=462.0); [0448]
4-{2-[7-(imidazo[1,2-a]pyridin-2-ylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]n-
on-3-yl]ethoxy}isophthalonitrile (m/z=429.0); [0449]
4-[2-(7-{2-[4-(tert-butoxy)phenoxy]ethyl}-9-oxa-3,7-diazabicyclo[3.3.1]no-
n-3-yl)ethoxy]isophthalonitrile (m/z=491.6); [0450]
4-(2-{7-[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo-[3-
.3.1]non-3-yl}ethoxy)isophthalonitrile (m/z=421.5); [0451]
4-(4-{7-[2-(1H-imidazol-4-yl)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl-
}butyl)benzonitrile (m/z=380.1); [0452]
4-{4-[7-(imidazo[1,2-a]pyridin-2-ylmethyl)-9-oxa-3,7-diazabicyclo[3.3.1]--
non-3-yl]butyl}benzonitrile (m/z=416.5); [0453]
4-{4-[7-(2-phenoxyethyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]butyl}-ben-
zonitrile (m/z=406.5); [0454]
4-(4-{7-[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]-9-oxa-3,7-diazabicyclo-[3-
.3.1]non-3-yl}butyl)benzonitrile (m/z=408.6); [0455]
4-[3-(7-{2-oxo-2-[4-(1-pyrrolidinyl)phenyl]ethyl}-9-oxa-3,7-diazabicyclo--
[3.3.1]non-3-yl)propoxy]benzonitrile (m/z=475);
4-(3-{7-[2-(4-hydroxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-
-3-yl}propoxy)benzonitrile (m/z=422); [0456]
4-(3-{7-[2-(4-methylphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-
-3-yl}propoxy)benzonitrile (m/=420); [0457]
4-(3-{7-[2-(4-methoxyphenyl)-2-oxoethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-no-
n-3-yl}propoxy)benzonitrile (m/z=436); [0458]
4-(3-{7-[2-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-oxoethyl]-9-oxa-3,7-diaza-
bicyclo[3.3.1]non-3-yl}propoxy)benzonitrile (m/z=464); [0459]
4-(2-{7-[2-(2,6-dimethylphenoxy)-1-methylethyl]-9-oxa-3,7-diazabicyclo[3.-
3.1]non-3-yl}ethoxy)benzonitrile (m/z=436); [0460]
4-(3-{7-[2-oxo-2-(3-oxo-3,4-dihydro-2H-1,4-benzoxazin-6-yl)ethyl]-9-oxa-3-
,7-diazabicyclo[3.3.1]non-3-yl}propoxy)benzonitrile (m/z=477);
[0461] tert-butyl
2-{7-[3-(4-cyanophenoxy)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}eth-
ylcarbamate (m/z=431); [0462]
N-(tert-butyl)-N'-(2-{7-[3-(4-cyanophenoxy)propyl]-9-oxa-3,7-diazabicyclo-
[3.3.1]non-3-yl}ethyl)urea (m/z=430); [0463] tert-butyl
2-({7-[2-(4-cyanophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl}met-
hyl)-1-pyrrolidinecarboxylate (m/z=457); [0464]
4-{[3-(7-benzyl-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl)propyl]amino}-benzo-
nitrile (m/z=377); [0465]
4-[(3-{7-[3-(4-cyanoanilino)propyl]-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl-
}propyl)amino]benzonitrile (m/z=445); [0466] tert-butyl
2-{7-[2-(4-nitrophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-3-yl}eth-
ylcarbamate (m/z=437);
[0467] tert-butyl
2-[7-(2-{4-[(methylsulfonyl)amino]phenoxy}ethyl)-9-oxa-3,7-diazabicyclo[3-
.3.1]non-3-yl]ethylcarbamate; [0468] tert-butyl
2-{7-[2-(4-aminophenoxy)ethyl]-9-oxa-3,7-diazabicyclo[3.3.1]-non-3-yl}eth-
ylcarbamate; [0469]
4-({3-[7-(phenylsulfonyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]propyl}-a-
mino)benzonitrile; and [0470]
4-({3-[7-(3,3-dimethyl-2-oxobutyl)-9-oxa-3,7-diazabicyclo[3.3.1]non-3-yl]-
propyl}amino)benzamide.
Example 13
[0471] Title compounds of the above Examples were tested in Test A
above and were found to exhibit D.sub.10 values of more than
6.0.
ABBREVIATIONS
[0472] Ac=acetyl [0473] API=atmospheric pressure ionisation (in
relation to MS) [0474] aq.=aqueous [0475] br=broad (in relation to
NMR) [0476] Bt=benzotriazole [0477] t-BuOH=tert-butanol [0478]
CI=chemical ionisation (in relation to MS) [0479]
mCPBA=meta-chloroperoxybenzoic acid [0480] d=doublet (in relation
to NMR) [0481] DBU=diazabicyclo[5.4.0]undec-7-ene [0482]
DCM=dichloromethane [0483] dd=doublet of doublets (in relation to
NMR) [0484] DMAP=4-dimethylaminopyridine [0485]
DMF=N,N-dimethylformamide [0486] DMSO=dimethylsulfoxide [0487]
EDC=1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide [0488] Et=ethyl
[0489] EtOAc=ethyl acetate [0490] eq.=equivalents [0491]
ES=electrospray (in relation to MS) [0492] FAB=fast atom
bombardment (in relation to MS) [0493] h=hour(s) [0494]
HCl=hydrochloric acid [0495]
HEPES=4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [0496]
HPLC=high performance liquid chromatography [0497] IMS=industrial
methylated spirits [0498] IPA=iso-propyl alcohol (propan-2-ol)
[0499] m=multiplet (in relation to NMR) [0500] Me=methyl [0501]
MeCN=acetonitrile [0502] MeOH=methanol [0503] min.=minute(s) [0504]
m.p.=melting point [0505] MS=mass spectroscopy [0506]
NADPH=nicotinamide adenine dinucleotide phosphate, reduced form
[0507] OAc=acetate [0508] Pd/C=palladium on carbon [0509] q=quartet
(in relation to NMR) [0510] rt=room temperature [0511] s=singlet
(in relation to NMR) [0512] t=triplet (in relation to NMR) [0513]
TEA=triethylamine [0514] THF=tetrahydrofuran [0515] tlc=thin layer
chromatography
[0516] Prefixes n-, s-, i-, t- and tert- have their usual meanings:
normal, secondary, iso, and tertiary.
* * * * *