U.S. patent application number 12/350814 was filed with the patent office on 2009-11-19 for novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of breast cancer.
This patent application is currently assigned to Compugen LTD. Invention is credited to Pinchas Akiva, Michal Ayalon-Soffer, Dvir Dahary, Alexander Diber, Naomi Keren, Zurit Levine, Amit Novik, Sarah Pollock, Shirley Sameah-Greenwald, Osnat Sella-Tavor, Ronen Shemesh, Maxim Shklar, Rotem Sorek, Amir Toporik, Shira Walach.
Application Number | 20090286957 12/350814 |
Document ID | / |
Family ID | 36816095 |
Filed Date | 2009-11-19 |
United States Patent
Application |
20090286957 |
Kind Code |
A1 |
Toporik; Amir ; et
al. |
November 19, 2009 |
NOVEL NUCLEOTIDE AND AMINO ACID SEQUENCES, AND ASSAYS AND METHODS
OF USE THEREOF FOR DIAGNOSIS OF BREAST CANCER
Abstract
Novel markers for breast cancer that are both sensitive and
accurate. These markers are overexpressed in breast cancer
specifically, as opposed to normal breast tissue. The measurement
of these markers, alone or in combination, in patient samples
provides information that the diagnostician can correlate with a
probable diagnosis of breast cancer. The markers of the present
invention, alone or in combination, show a high degree of
differential detection between breast cancer and non-cancerous
states.
Inventors: |
Toporik; Amir; (Azur,
IL) ; Dahary; Dvir; (Tel-Aviv, IL) ; Sorek;
Rotem; (Rechovot, IL) ; Pollock; Sarah;
(Tel-Aviv, IL) ; Levine; Zurit; (Herzlia, IL)
; Akiva; Pinchas; (Ramat-Gan, IL) ; Diber;
Alexander; (Rochon-LeZion, IL) ; Novik; Amit;
(Beit-HaSharon, IL) ; Sella-Tavor; Osnat; (Kfar
Kish, IL) ; Ayalon-Soffer; Michal; (Ramat-HaSharon,
IL) ; Walach; Shira; (Hod-HaSharon, IL) ;
Sameah-Greenwald; Shirley; (Kfar-Saba, IL) ; Shemesh;
Ronen; (Modiln, IL) ; Keren; Naomi; (Givat
Shmuel, IL) ; Shklar; Maxim; (Tel-Aviv, IL) |
Correspondence
Address: |
MINTZ, LEVIN, COHN, FERRIS, GLOVSKY AND POPEO, P.C
ONE FINANCIAL CENTER
BOSTON
MA
02111
US
|
Assignee: |
Compugen LTD
Tel Aviv
IL
|
Family ID: |
36816095 |
Appl. No.: |
12/350814 |
Filed: |
January 8, 2009 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
11711827 |
Feb 28, 2007 |
7528243 |
|
|
12350814 |
|
|
|
|
11043842 |
Jan 27, 2005 |
|
|
|
11711827 |
|
|
|
|
60620916 |
Oct 22, 2004 |
|
|
|
60628123 |
Nov 17, 2004 |
|
|
|
60621131 |
Oct 25, 2004 |
|
|
|
60620917 |
Oct 22, 2004 |
|
|
|
60628101 |
Nov 17, 2004 |
|
|
|
60620874 |
Oct 22, 2004 |
|
|
|
60628134 |
Nov 17, 2004 |
|
|
|
60620924 |
Oct 22, 2004 |
|
|
|
60628111 |
Nov 17, 2004 |
|
|
|
60620853 |
Oct 22, 2004 |
|
|
|
60628112 |
Nov 17, 2004 |
|
|
|
60620974 |
Oct 22, 2004 |
|
|
|
60628145 |
Nov 17, 2004 |
|
|
|
60620656 |
Oct 22, 2004 |
|
|
|
60628251 |
Nov 17, 2004 |
|
|
|
60620975 |
Oct 22, 2004 |
|
|
|
60628178 |
Nov 17, 2004 |
|
|
|
60628231 |
Nov 17, 2004 |
|
|
|
60620918 |
Oct 22, 2004 |
|
|
|
60628156 |
Nov 17, 2004 |
|
|
|
60628167 |
Nov 17, 2004 |
|
|
|
60621004 |
Oct 22, 2004 |
|
|
|
60539129 |
Jan 27, 2004 |
|
|
|
60539128 |
Jan 27, 2004 |
|
|
|
Current U.S.
Class: |
530/350 |
Current CPC
Class: |
C12Q 1/6886 20130101;
C12Q 2600/112 20130101; C07K 14/47 20130101; C12Q 2600/158
20130101; G01N 33/57415 20130101 |
Class at
Publication: |
530/350 |
International
Class: |
C07K 14/435 20060101
C07K014/435 |
Claims
1. An isolated polypeptide comprising an amino acid sequence at
least about 90% homologous to the amino acid sequence of
T94936_PEA.sub.--1_P3 (SEQ ID NO:207).
2. The polypeptide of claim 1, comprising a first portion having an
amino acid sequence being at least about 90% homologous to amino
acids 1-122 of T94936_PEA.sub.--1_P3 SEQ ID NO:207, and a second
portion having amino acid sequence being at least about 80%, 85%,
90%, 95% or 100% homologous to SEQ ID NO:1019, wherein the first
amino acid sequence and the second amino acid sequence are
contiguous.
3. The polypeptide of claim 1, wherein said polypeptide comprises
an amino acid sequence at least 95% homologous to SEQ ID
NO:207.
4. The polypeptide of claim 1, wherein said polypeptide comprises
the amino acid sequence of SEQ ID NO:207.
5. The polypeptide of claim 2, wherein said first portion comprises
an amino acid sequence at least 95% homologous to amino acids 1-122
of SEQ ID NO:207.
6. The polypeptide of claim 2, wherein said first portion comprises
the amino acid sequence of amino acids 1-122 of SEQ ID NO:207.
7. The polypeptide of claim 2, wherein said second portion
comprises an amino acid sequence at least 95% homologous to SEQ ID
NO:1019.
8. The polypeptide of claim 2, wherein said second portion
comprises the amino acid sequence of SEQ ID NO:1019.
9. The isolated polypeptide of claim 1, wherein said polypeptide is
attached to a label.
10. The isolated polypeptide of claim 1, wherein said polypeptide
is isolated from a cancer cell.
11. The isolated polypeptide of claim 1, wherein said polypeptide
is recombinantly expressed.
12. The isolated polypeptide of claim 11, wherein said polypeptide
is isolated from an E. coli cell.
13. An antigenic fragment of the polypeptide of claim 1.
14. An isolated polypeptide comprising an amino acid sequence at
least about 80%, 85%, 90%, or 95% homologous to the amino acid
sequence as set forth in SEQ ID NO: 1019.
15. The polypeptide of claim 14, comprising the sequence as set
forth in SEQ ID NO: 1019.
16. The polypeptide of claim 14, wherein said polypeptide is
attached to a label.
Description
CROSS-REFERENCE TO RELATED APPLICATION(S)
[0001] This application is related to novel nucleotide and amino
acid sequences, and assays and methods of use thereof for diagnosis
of breast cancer, and claims priority to and incorporates herein by
reference (in their entirety) each of the corresponding non-U.S.
provisional applications and their corresponding U.S. PROVISIONAL
APPLICATIONS noted below:
[0002] Non-Provisional Application Ser. No. 11/043,842 filed on
JAN. 27, 2005 and 60/620,916 filed OCT. 22, 2004--Differential
Expression of Markers in Colon Cancer
[0003] Application Ser. No. 60/628,123 filed NOV. 17,
2004--Differential Expression of Markers in Colon Cancer II
[0004] Application No. 60/621,131 filed OCT. 25, 2004--Diagnostic
Markers for Colon Cancer, and Assays and Methods of Use Thereof
[0005] Application No. 60/620,917 filed OCT. 22, 2004--Differential
Expression of Markers in Breast Cancer
[0006] Application No. 60/628,101 filed NOV. 17, 2004--Differential
Expression of Markers in Breast Cancer II
[0007] Application No. 60/620,874 filed OCT. 22, 2004--Differential
Expression of Markers in Ovarian Cancer
[0008] Application No. 60/628,134 filed NOV. 17, 2004--Differential
Expression of Markers in Ovarian Cancer II
[0009] Application No. 60/620,924 filed OCT. 22, 2004--Differential
Expression of Markers in Stomach Cancer
[0010] Application No. 60/628,111 filed NOV. 17, 2004--Differential
Expression of Markers in Stomach Cancer II
[0011] Application No. 60/620,853 filed OCT. 22,
2004-28814-Differential Expression of Markers in Lung Cancer
[0012] Application No. 60/628,112 filed NOV. 17, 2004--Differential
Expression of Markers in Lung Cancer II
[0013] Application No. 60/620,974 filed OCT. 22, 2004--Differential
Expression of Markers in Pancreatic Cancer
[0014] Application No. 60/628,145 filed NOV. 17, 2004--Differential
Expression of Markers in Pancreatic Cancer II
[0015] Application No. 60/620,656 filed OCT. 22, 2004--Differential
Expression of Markers in Prostate Cancer
[0016] Application No. 60/628,251 filed NOV. 17, 2004--Differential
Expression of Markers in Prostate Cancer II
[0017] Application No. 60/620,975 filed OCT. 22, 2004--Differential
Expression of Markers in Brain Cancer
[0018] Application No. 60/628,178 filed NOV. 17, 2004--Differential
Expression of Markers in Brain Cancer II
[0019] Application No. 60/628,231 filed NOV. 17, 2004--Novel
Diagnostic Serum Markers, and Assays and Methods of Use Thereof
[0020] Application No. 60/620,918 filed OCT. 22, 2004--Diagnostic
Markers for Renal Cancer, and Assays and Methods of Use Thereof
[0021] Application No. 60/628,156 filed NOV. 17, 2004--Diagnostic
Markers for Renal Cancer, and Assays and Methods of Use Thereof
II
[0022] Application No. 60/628,167 filed NOV. 17, 2004--Differential
Expression of Markers in Bladder Cancer II
[0023] Application No. 60/621,004 filed OCT. 22, 2004--Differential
Expression of Markers in Skin and Epithelial Cancer II
[0024] Application Ser. No. ----NA----- filed NOV. 17, 2004--Novel
Diagnostic Markers, And Assays and Methods of Use Thereof
[0025] Application No. 60/539,129 filed JAN. 27, 2004--Methods and
Systems for Annotating Biomolecular Sequences
[0026] Application No. 60/539,128 filed JAN. 27, 2004--Evolutionary
Conserved Spliced Sequences and Methods and Systems for Identifying
Thereof and Application Ser. No. 11/043,842 filed JAN. 27, 2005
FIELD OF THE INVENTION
[0027] The present invention is related to novel nucleotide and
protein sequences that are diagnostic markers for breast cancer,
and assays and methods of use thereof. The "Sequence Listing"
recited on the computer readable form (CFR) CD filed herewith is
incorporated herein by reference in its entirety.
BACKGROUND OF THE INVENTION
[0028] Breast cancer is the most commonly occurring cancer in
women, comprising almost a third of all malignancies in females. It
is the leading cause of death for women between the ages 40-55 in
the United States and one out of 8 females in the United States
will develop breast cancer at some point in her life.
[0029] The death rate from breast cancer has been slowly declining
over the past decade, partially due do the usage of molecular
markers that facilitate the discovery, tumor typing (and therefore
choice of treatment), response to treatment and recurrence.
[0030] The most widely used serum markers for breast cancers are
Mucin 1 (measured as CA 15-3) and CEA (CarcinoEmbryonic Antigen).
Mucin 1 (MUC1) is present on the apical surface of normal
epithelial cells. Its extracellular domain consists of a heavily
O-linked glycosylated peptide core made up of variable number of
multiple repeats of 20 amino acid sequence referred to as VNTR
(Variable Number Tandem Repeat). This variability results in
natural polymorphism of MUC1. Each VNTR has five potential
O-linkage sites. The breast cancer disease state alters the enzymes
which glycosylate Mucin 1 and therefore the polysaccharide side
chains of tumor associated MUC1 are generally shorter than those on
the normally expressed molecule. Both aberrant and up-regulated
expression of MUC1 are features of malignancy and MUC1 related
markers are based on it. Though CA 15-3 is a broadly used marker
for breast cancer, a combination of CA 15-3 and CEA is more
sensitive than using a single marker.
[0031] For the purpose of monitoring therapeutic response, CA 15-3,
CEA and ESR (Erythrocyte Sedimentation Rate) are used as a panel,
leading to over 90% of patients biochemically assessable. Serum
markers used to monitor therapeutic response in patients with
metastatic breast cancer are associated with the "spike
phenomenon". It is an initial transient rise of tumor marker levels
which can be seen in up to 30% of responders in the first 3 months
of commencing a therapy. It is important not to interpret this as a
sign of disease progression leading to premature change of an
effective therapy.
[0032] CA 27.29 is a new monoclonal antibody directed against a
different part of MUC1 and it is a newer marker than CA 15-3. It
detects a different glycosylation pattern of MUC1, as compared with
CA 15-3. CA 27.29 is the first FDA-approved blood test for breast
cancer recurrence. Because of superior sensitivity and specificity,
CA 27.29 has supplanted CA 15-3 as the preferred tumor marker in
breast cancer. The CA 27.29 level is elevated in approximately one
third of women with early-stage breast cancer (stage I or II) and
in two thirds of women with late-stage disease (stage III or IV).
CA 27.29 lacks predictive value in the earliest stages of breast
cancer and thus has no role in screening for or diagnosing the
malignancy. CA 27.29 also can be found in patients with benign
disorders of the breast, liver, and kidney, and in patients with
ovarian cysts. However, CA 27.29 levels higher than 100 units per
mL are rare in benign conditions.
[0033] Recently Estrogen 2 (beta) was shown to have a diagnostic
role in breast cancer. It has been shown that the expression of the
`cx` variant of Estrogen 2 is correlated with response to Hormone
adjuvant therapy. In addition it has been shown it may assist in
better characterization of ER-1 positive breast cancers (together
with progesterone receptor).
[0034] HER-2 (also known as c-erbB2) is a membrane proto-oncogene
with intrinsic tyrosine kinase activity. Tumor expressing HER-2 are
associated with shorter survival, shorter time-to-relapse and an
overall worse prognosis. Tumors expressing HER-2 can be targeted
with Trastuzumab--a biological adjuvant therapy which blocks the
growth promoting action of HER-2. The ImmunoHistoChemistry (IHC)
and Fluorescence In Situ Hybridization (FISH) tests are used to
detect HER2:1.IHC: The most common test used to check HER2 status
is an ImmunoHistoChemistry (IHC) test. The IHC test measures the
protein made by the HER2 gene. 2.FISH: This test measures the
number of copies of the HER2 gene present in the tumor cell.
[0035] Measurement of the extracellular domain of HER-2 has been
reported to show a better assessment of response to chemotherapy
than a biochemical index score based on measurement of CA 15.3, CEA
and ESR in a small series of patient. That finding is yet to be
confirmed in a larger group of patient with HER-2 expressing
tumors.
[0036] Other molecular markers, mainly used for the diagnosis for
cancers other than breast cancer were shown to have a diagnostic
potential in breast cancer. For example, CA125 which is a major
marker for ovarian cancer is also associated with breast cancer.
High levels of CA 19-9, a major marker for colorectal and
pancreatic cancers, can be found in breast cancer. Overall, these
markers are not frequently used for the detection of breast cancer
to due their inferiority compared with other markers already
described.
[0037] Panels of markers for the diagnosis and typing of breast
cancer are being used by pathologists, including both markers
described above and additional markers, such as
immunohistochemistry markers that have been shown to have a
beneficial value for the diagnosis of breast cancer, including PCNA
and Ki-67 are maybe the most important and highly used
immunohistochemistry markers for breast cancer. Other markers as
E-Cadherin, Cathepsin D and TFF1 are also used for that
purpose.
[0038] Despite relevant research efforts and the identification of
many putative good prognosticators, few of them are proving
clinically useful for identifying patients at minimal risk of
relapse, patients with a worse prognosis, or patients likely to
benefit from specific treatments. Most of them, such as epidermal
growth factor receptor, cyclin E, p53 (this mutation is present in
approximately 40% of human breast cancers as an acquired defect),
bcl-2, vascular endothelial growth factor, urokinase-type
plasminogen activator-1 and the anti-apoptosis protein survivin,
are suggested for possible inclusion in the category of biomarkers
with a high level of clinico-laboratory effectiveness. However, no
single biomarker was able to identify those patients with the best
(or worst) prognosis or those patients who would be responsive to a
given therapy. High level cyclin E expression has been associated
with the initiation or progression of different human cancers, in
particular breast cancer but also leukemia, lymphoma and others.
Cyclin-E expression level in the breast cancer was found to be a
very strong indicator for prognosis, stronger than any other
biological marker.
[0039] There are some non-cancerous pathological conditions which
represent an increased risk factor for development breast cancer.
Non-limiting examples of these conditions include:
[0040] Ductal hyperplasia without atypia. It is the most frequently
encountered breast biopsy result that is associated with increased
risk of future development of breast cancer (2 fold increased
risk). In particular, the loss of expression of transforming growth
factor beta receptor II in the affected epithelial cells is
associated with an increased risk of invasive breast cancer.
[0041] Atypical hyperplasia. Women having atypical hyperplasia with
over-expression of HER-2 have a greater than 7-fold increased risk
of developing invasive breast carcinoma, as compared with women
with non-proliferative benign breast lesions and no evidence of
HER-2 amplification.
[0042] These pathological conditions should be effectively
diagnosed and monitored in order to facilitate early detection of
breast cancer.
SUMMARY OF THE INVENTION
[0043] The background art does not teach or suggest markers for
breast cancer that are sufficiently sensitive and/or accurate,
alone or in combination.
[0044] The present invention overcomes these deficiencies of the
background art by providing novel markers for breast cancer that
are both sensitive and accurate. These markers are overexpressed in
breast cancer specifically, as opposed to normal breast tissue. The
measurement of these markers, alone or in combination, in patient
(biological) samples provides information that the diagnostician
can correlate with a probable diagnosis of breast cancer. The
markers of the present invention, alone or in combination, show a
high degree of differential detection between breast cancer and
non-cancerous states.
[0045] According to preferred embodiments of the present invention,
examples of suitable biological samples which may optionally be
used with preferred embodiments of the present invention include
but are not limited to blood, serum, plasma, blood cells, urine,
sputum, saliva, stool, spinal fluid or CSF, lymph fluid, the
external secretions of the skin, respiratory, intestinal, and
genitourinary tracts, tears, milk, neuronal tissue, breast tissue,
any human organ or tissue, including any tumor or normal tissue,
any sample obtained by lavage (for example of the bronchial system
or of the breast ductal system), and also samples of in vivo cell
culture constituents. In a preferred embodiment, the biological
sample comprises breast tissue and/or a serum sample and/or a urine
sample and/or a milk sample and/or any other tissue or liquid
sample. The sample can optionally be diluted with a suitable eluant
before contacting the sample to an antibody and/or performing any
other diagnostic assay.
[0046] Information given in the text with regard to cellular
localization was determined according to four different software
programs: (i) tmhmm (from Center for Biological Sequence Analysis,
Technical University of Denmark DTU,
cbs.dtu.dk/services/TMHMM/TMHMM2.0b.guide.php) or (ii) tmpred (from
EMBnet, maintained by the ISREC Bionformatics group and the LICR
Information Technology Office, Ludwig Institute for Cancer
Research, Swiss Institute of Bioinformatics,
ch.embnet.org/software/TMPRED_form.html) for transmembrane region
prediction; (iii) signalp_hmm or (iv) signalp_nn (both from Center
for Biological Sequence Analysis, Technical University of Denmark
DTU, cbs.dtu.dk/services/SignalP/background/prediction.php) for
signal peptide prediction. The terms "signalp_hmm" and "signalp_nn"
refer to two modes of operation for the program SignalP: hmm refers
to Hidden Markov Model, while nn refers to neural networks.
Localization was also determined through manual inspection of known
protein localization and/or gene structure, and the use of
heuristics by the individual inventor. In some cases for the manual
inspection of cellular localization prediction inventors used the
ProLoc computational platform [Einat Hazkani-Covo, Erez Levanon,
Galit Rotman, Dan Graur and Amit Novik; (2004) "Evolution of
multicellularity in metazoa: comparative analysis of the
subcellular localization of proteins in Saccharomyces, Drosophila
and Caenorhabditis." Cell Biology International 2004;
28(3):171-8.], which predicts protein localization based on various
parameters including, protein domains (e.g., prediction of
trans-membranous regions and localization thereof within the
protein), pI, protein length, amino acid composition, homology to
pre-annotated proteins, recognition of sequence patterns which
direct the protein to a certain organelle (such as, nuclear
localization signal, NLS, mitochondria localization signal), signal
peptide and anchor modeling and using unique domains from Pfam that
are specific to a single compartment.
[0047] Information is given in the text with regard to SNPs (single
nucleotide polymorphisms). A description of the abbreviations is as
follows. "T.fwdarw.C", for example, means that the SNP results in a
change at the position given in the table from T to C. Similarly,
"M.fwdarw.Q", for example, means that the SNP has caused a change
in the corresponding amino acid sequence, from methionine (M) to
glutamine (Q). If, in place of a letter at the right hand side for
the nucleotide sequence SNP, there is a space, it indicates that a
frameshift has occurred. A frameshift may also be indicated with a
hyphen (-). A stop codon is indicated with an asterisk at the right
hand side (*). As part of the description of an SNP, a comment may
be found in parentheses after the above description of the SNP
itself. This comment may include an FTId, which is an identifier to
a SwissProt entry that was created with the indicated SNP. An FTId
is a unique and stable feature identifier, which allows
construction of links directly from position-specific annotation in
the feature table to specialized protein-related databases. The
FTId is always the last component of a feature in the description
field, as follows: FTId=XXX_number, in which XXX is the 3-letter
code for the specific feature key, separated by an underscore from
a 6-digit number. In the table of the amino acid mutations of the
wild type proteins of the selected splice variants of the
invention, the header of the first column is "SNP position(s) on
amino acid sequence", representing a position of a known mutation
on amino acid sequence. SNPs may optionally be used as diagnostic
markers according to the present invention, alone or in combination
with one or more other SNPs and/or any other diagnostic marker.
Preferred embodiments of the present invention comprise such SNPs,
including but not limited to novel SNPs on the known (WT or wild
type) protein sequences given below, as well as novel nucleic acid
and/or amino acid sequences formed through such SNPs, and/or any
SNP on a variant amino acid and/or nucleic acid sequence described
herein.
[0048] Information given in the text with regard to the Homology to
the known proteins was determined by Smith-Waterman version 5.1.2
using special (non default) parameters as follows: TABLE-US-00001
model=sw.model GAPEXT=0 GAPOP=100.0 MATRIX=blosum100
[0049] Information is given with regard to overexpression of a
cluster in cancer based on ESTs. A key to the p values with regard
to the analysis of such overexpression is as follows:
library-based statistics: P-value without including the level of
expression in cell-lines (P1) library based statistics: P-value
including the level of expression in cell-lines (P2) EST clone
statistics: P-value without including the level of expression in
cell-lines (SP1) EST clone statistics: predicted overexpression
ratio without including the level of expression in cell-lines (R3)
EST clone statistics: P-value including the level of expression in
cell-lines (SP2) EST clone statistics: predicted overexpression
ratio including the level of expression in cell-lines (R4)
[0050] Library-based statistics refer to statistics over an entire
library, while EST clone statistics refer to expression only for
ESTs from a particular tissue or cancer.
[0051] Information is given with regard to overexpression of a
cluster in cancer based on microarrays. As a microarray reference,
in the specific segment paragraphs, the unabbreviated tissue name
was used as the reference to the type of chip for which expression
was measured. There are two types of microarray results: those from
microarrays prepared according to a design by the present
inventors, for which the microarray fabrication procedure is
described in detail in Materials and Experimental Procedures
section herein; and those results from microarrays using Affymetrix
technology. As a microarray reference, in the specific segment
paragraphs, the unabbreviated tissue name was used as the reference
to the type of chip for which expression was measured. For
microarrays prepared according to a design by the present
inventors, the probe name begins with the name of the cluster
(gene), followed by an identifying number. Oligonucleotide
microarray results taken from Affymetrix data were from chips
available from Affymetrix Inc, Santa Clara, Calif., USA (see for
example data regarding the Human Genome U1133 (HG-U133) Set at
affymetrix.com/products/arrays/specific/hgu133.affx; GeneChip Human
Genome U133A 2.0 Array at
affymetrix.com/products/arrays/specific/hgu-133av2.affx; and Human
Genome U133 Plus 2.0 Array at
affymetrix.com/products/arrays/specific/hgu133 plus.affx). The
probe names follow the Affymetrix naming convention. The data is
available from NCBI Gene Expression Omnibus (see
ncbi.nlm.nih.gov/projects/geo/ and Edgar et al, Nucleic Acids
Research, 2002, Vol. 30, No. 1207-210). The dataset (including
results) is available from
ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE1133 for the Series
GSE1133 database (published on March 2004); a reference to these
results is as follows: Su et al (Proc Natl Acad Sci USA. 2004 Apr.
20; 101(16):6062-7. Epub 2004 Apr. 9). The probes designed
according to the present inventors are listed below.
TABLE-US-00002>Z21368.sub.--0.sub.--0.sub.--61857
AGTTCATCCTTCTTCAGTGTGACCAGTAAATTCTTCCCATACTCTTGAAG (SEQ ID
NO:895)>HUMGRP5E.sub.--0.sub.--0.sub.--16630
GCTGATATGGAAGTTGGGGAATCTGAATTGCCAGAGAATCTTGGGAAGAG (SEQ ID
NO:896)>HUMGRP5E.sub.--0.sub.--2.sub.--0
TCTCATAGAAGCAAAGGAGAACAGAAACCACCAGCCACCTCAACCCAAGG (SEQ ID
NO:897)>HSENA78.sub.--0.sub.--1.sub.--0
TGAAGAGTGTGAGGAAAACCTATGTTTGCCGCTTAAGCTTTCAGCTCAGC (SEQ ID
NO:898)>M85491.sub.--0.sub.--0.sub.--25999
GACATCTTTGCATATCATGTCAGAGCTATAACATCATTGTGGAGAAGCTC (SEQ ID
NO:899)>M85491.sub.--0.sub.--14.sub.--0
GTCATGAAAATCAACACCGAGGTGCGGAGCTTCGGACCTGTGTCCCGCAG (SEQ ID
NO:900)>HSSTROL3.sub.--0.sub.--0.sub.--12518
ATGAGAGTAACCTCACCCGTGCACTAGTTTACAGAGCATTCACTGCCCCA (SEQ ID
NO:901)>HSSTROL3.sub.--0.sub.--0.sub.--12517
CAGAGATGAGAGCCTGGAGCATTGCAGATGCCAGGGACTTCACAAATGAA (SEQ ID
NO:902)>HUMCA1XIA.sub.--0.sub.--0.sub.--14909
GCTGCAATCTAAGTTTCGGAATACTTATACCACTCCAGAAATAATCCTCG (SEQ ID
NO:903)>HUMCA1XIA.sub.--0.sub.--18.sub.--0
TTCAGAACTGTTAACATCGCTGACGGGAAGTGGCATCGGGTAGCAATCAG (SEQ ID
NO:904)>R20779.sub.--0.sub.--0.sub.--30670
CCGCGTTGCTTCTAGAGGCTGAATGCCTTTCAAATGGAGAAGGCTTCCAT (SEQ ID
NO:905)>HSS100PCB.sub.--0.sub.--0.sub.--12280
CTCAAAATGAAACTCCCTCTCGCAGAGCACAATTCCAATTCGCTCTAAAA (SEQ ID
NO:906)>HSCOC4.sub.--0.sub.--0.sub.--9892
AAGGACCAGAGTCCATGCCAAGACCACCCTTCAGCTTCCAAGGCCCTCCA (SEQ ID
NO:907)>HSCOC4.sub.--0.sub.--39.sub.--0
ATCCTCCAGCCATGAGGCTGCTCTGGGGGCTGATCTGGGCATCCAGCTTC (SEQ ID
NO:908)>HSCOC4.sub.--0.sub.--0.sub.--9883
CCTGTTTGCTCTGACACCAACTTCCTACCCTCTCAGCCTCAAAGTAACTC (SEQ ID
NO:909)>HSCOC4.sub.--0.sub.--0.sub.--9885
GCTGAGGTGTGGCCGAGGACCTGACCATCTGGAAGTGTGAAAATCCCCTT (SEQ ID
NO:910)>T11628.sub.--0.sub.--9.sub.--0
ACAAGATCCCCGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAG (SEQ ID
NO:911)>T11628.sub.--0.sub.--0.sub.--45174
TAAACAATCAAAGAGCATGTTGGCCTGGTCCTTTGCTAGGTACTGTAGAG (SEQ ID
NO:912)>T11628.sub.--0.sub.--0.sub.--45161
TGCCTCGCCACAATGGCACCTGCCCTAAAATAGCTTCCCATGTGAGGGCT (SEQ ID
NO:913)>M78076.sub.--0.sub.--7.sub.--0
GAGAAGATGAACCCGCTGGAACAGTATGAGCGAAAGGTGAATGCGTCTGT (SEQ ID
NO:914)>HSMUC1A.sub.--0.sub.--37.sub.--0
AAAAGGAGACTTCGGCTACCCAGAGAAGTTCAGTGCCCAGCTCTACTGAG (SEQ ID
NO:915)>HSMUC1A.sub.--0.sub.--0.sub.--11364
AAAGGCTGGCATAGGGGGAGGTTTCCCAGGTAGAAGAAGAAGTGTCAGCA (SEQ ID
NO:916)>HSMUC1A.sub.--0.sub.--0.sub.--11365
AATTAACCCTTTGAGAGCTGGCCAGGACTCTGGACTGATTACCCCAGCCT (SEQ ID
NO:917)
[0052] The following list of abbreviations for tissues was used in
the TAA histograms. The term "TAA" stands for "Tumor Associated
Antigen", and the TAA histograms, given in the text, represent the
cancerous tissue expression pattern as predicted by the biomarkers
selection engine, as described in detail in examples 1-5 below.
TABLE-US-00003 "BONE" for "bone"; "COL" for "colon"; "EPI" for
"epithelial"; "GEN" for "general"; "LIVER" for "liver"; "LUN" for
"lung"; "LYMPH" for "lymph nodes"; "MARROW" for "bone marrow";
"OVA" for "ovary"; "PANCREAS" for "pancreas"; "PRO" for "prostate";
"STOMACH" for "stomach"; "TCELL" for "T cells"; "THYROID" for
"Thyroid"; "MAM" for "breast"; "BRAIN" for "brain"; "UTERUS" for
"uterus"; "SKIN" for "skin"; "KIDNEY" for "kidney"; "MUSCLE" for
"muscle"; "ADREN" for "adrenal"; "HEAD" for "head and neck";
"BLADDER" for "bladder";
[0053] It should be noted that the terms "segment", "seg" and
"node" are used interchangeably in reference to nucleic acid
sequences of the present invention, they refer to portions of
nucleic acid sequences that were shown to have one or more
properties as described below. They are also the building blocks
that were used to construct complete nucleic acid sequences as
described in greater detail below. Optionally and preferably, they
are examples of oligonucleotides which are embodiments of the
present invention, for example as amplicons, hybridization units
and/or from which primers and/or complementary oligonucleotides may
optionally be derived, and/or for any other use.
[0054] As used herein the phrase "breast cancer" refers to cancers
of the breast or surrounding tissue, including but not limited to
ductal carcinoma (in-situ or invasive), lobular carcinoma (in-situ
or invasive), inflammatory breast cancer, mucinous carcinoma,
tubular carcinoma, or Paget's disease of the nipple, as well as
conditions that are indicative of a higher risk factor for later
development of breast cancer, including but not limited to ductal
hyperplasia without atypia and atypical hyperplasia, referred to
herein collectively as "indicative conditions".
[0055] The term "marker" in the context of the present invention
refers to a nucleic acid fragment, a peptide, or a polypeptide,
which is differentially present in a sample taken from subjects
(patients) having breast cancer (or one of the above indicative
conditions) as compared to a comparable sample taken from subjects
who do not have breast cancer (or one of the above indicative
conditions).
[0056] The phrase "differentially present" refers to differences in
the quantity of a marker present in a sample taken from patients
having breast cancer (or one of the above indicative conditions) as
compared to a comparable sample taken from patients who do not have
breast cancer (or one of the above indicative conditions). For
example, a nucleic acid fragment may optionally be differentially
present between the two samples if the amount of the nucleic acid
fragment in one sample is significantly different from the amount
of the nucleic acid fragment in the other sample, for example as
measured by hybridization and/or NAT-based assays. A polypeptide is
differentially present between the two samples if the amount of the
polypeptide in one sample is significantly different from the
amount of the polypeptide in the other sample. It should be noted
that if the marker is detectable in one sample and not detectable
in the other, then such a marker can be considered to be
differentially present.
[0057] As used herein the phrase "diagnostic" means identifying the
presence or nature of a pathologic condition. Diagnostic methods
differ in their sensitivity and specificity. The "sensitivity" of a
diagnostic assay is the percentage of diseased individuals who test
positive (percent of "true positives"). Diseased individuals not
detected by the assay are "false negatives." Subjects who are not
diseased and who test negative in the assay are termed "true
negatives." The "specificity" of a diagnostic assay is 1 minus the
false positive rate, where the "false positive" rate is defined as
the proportion of those without the disease who test positive.
While a particular diagnostic method may not provide a definitive
diagnosis of a condition, it suffices if the method provides a
positive indication that aids in diagnosis.
[0058] As used herein the phrase "diagnosing" refers to classifying
a disease or a symptom, determining a severity of the disease,
monitoring disease progression, forecasting an outcome of a disease
and/or prospects of recovery. The term "detecting" may also
optionally encompass any of the above.
[0059] Diagnosis of a disease according to the present invention
can be effected by determining a level of a polynucleotide or a
polypeptide of the present invention in a biological sample
obtained from the subject, wherein the level determined can be
correlated with predisposition to, or presence or absence of the
disease. It should be noted that a "biological sample obtained from
the subject" may also optionally comprise a sample that has not
been physically removed from the subject, as described in greater
detail below.
[0060] As used herein, the term "level" refers to expression levels
of RNA and/or protein or to DNA copy number of a marker of the
present invention.
[0061] Typically the level of the marker in a biological sample
obtained from the subject is different (i.e., increased or
decreased) from the level of the same variant in a similar sample
obtained from a healthy individual (examples of biological samples
are described herein).
[0062] Numerous well known tissue or fluid collection methods can
be utilized to collect the biological sample from the subject in
order to determine the level of DNA, RNA and/or polypeptide of the
variant of interest in the subject.
[0063] Examples include, but are not limited to, fine needle
biopsy, needle biopsy, core needle biopsy and surgical biopsy
(e.g., brain biopsy), and lavage. Regardless of the procedure
employed, once a biopsy/sample is obtained the level of the variant
can be determined and a diagnosis can thus be made.
[0064] Determining the level of the same variant in normal tissues
of the same origin is preferably effected along-side to detect an
elevated expression and/or amplification and/or a decreased
expression, of the variant as opposed to the normal tissues.
[0065] A "test amount" of a marker refers to an amount of a marker
in a subject's sample that is consistent with a diagnosis of breast
cancer (or one of the above indicative conditions). A test amount
can be either in absolute amount (e.g., microgram/ml) or a relative
amount (e.g., relative intensity of signals).
[0066] A "control amount" of a marker can be any amount or a range
of amounts to be compared against a test amount of a marker. For
example, a control amount of a marker can be the amount of a marker
in a patient with breast cancer (or one of the above indicative
conditions) or a person without breast cancer (or one of the above
indicative conditions). A control amount can be either in absolute
amount (e.g., microgram/ml) or a relative amount (e.g., relative
intensity of signals). "Detect" refers to identifying the presence,
absence or amount of the object to be detected.
[0067] A "label" includes any moiety or item detectable by
spectroscopic, photo chemical, biochemical, immunochemical, or
chemical means. For example, useful labels include .sup.32P,
.sup.35S, fluorescent dyes, electron-dense reagents, enzymes (e.g.,
as commonly used in an ELISA), biotin-streptavadin, dioxigenin,
haptens and proteins for which antisera or monoclonal antibodies
are available, or nucleic acid molecules with a sequence
complementary to a target. The label often generates a measurable
signal, such as a radioactive, chromogenic, or fluorescent signal,
that can be used to quantify the amount of bound label in a sample.
The label can be incorporated in or attached to a primer or probe
either covalently, or through ionic, van der Waals or hydrogen
bonds, e.g., incorporation of radioactive nucleotides, or
biotinylated nucleotides that are recognized by streptavadin. The
label may be directly or indirectly detectable. Indirect detection
can involve the binding of a second label to the first label,
directly or indirectly. For example, the label can be the ligand of
a binding partner, such as biotin, which is a binding partner for
streptavadin, or a nucleotide sequence, which is the binding
partner for a complementary sequence, to which it can specifically
hybridize. The binding partner may itself be directly detectable,
for example, an antibody may be itself labeled with a fluorescent
molecule. The binding partner also may be indirectly detectable,
for example, a nucleic acid having a complementary nucleotide
sequence can be a part of a branched DNA molecule that is in turn
detectable through hybridization with other labeled nucleic acid
molecules (see, e.g., P. D. Fahrlander and A. Klausner,
Bio/Technology 6:1165 (1988)). Quantitation of the signal is
achieved by, e.g., scintillation counting, densitometry, or flow
cytometry.
[0068] Exemplary detectable labels, optionally and preferably for
use with immunoassays, include but are not limited to magnetic
beads, fluorescent dyes, radiolabels, enzymes (e.g., horse radish
peroxide, alkaline phosphatase and others commonly used in an
ELISA), and calorimetric labels such as colloidal gold or colored
glass or plastic beads. Alternatively, the marker in the sample can
be detected using an indirect assay, wherein, for example, a
second, labeled antibody is used to detect bound marker-specific
antibody, and/or in a competition or inhibition assay wherein, for
example, a monoclonal antibody which binds to a distinct epitope of
the marker are incubated simultaneously with the mixture.
[0069] "Immunoassay" is an assay that uses an antibody to
specifically bind an antigen. The immunoassay is characterized by
the use of specific binding properties of a particular antibody to
isolate, target, and/or quantify the antigen.
[0070] The phrase "specifically (or selectively) binds" to an
antibody or "specifically (or selectively) immunoreactive with,"
when referring to a protein or peptide (or other epitope), refers
to a binding reaction that is determinative of the presence of the
protein in a heterogeneous population of proteins and other
biologics. Thus, under designated immunoassay conditions, the
specified antibodies bind to a particular protein at least two
times greater than the background (non-specific signal) and do not
substantially bind in a significant amount to other proteins
present in the sample. Specific binding to an antibody under such
conditions may require an antibody that is selected for its
specificity for a particular protein. For example, polyclonal
antibodies raised to seminal basic protein from specific species
such as rat, mouse, or human can be selected to obtain only those
polyclonal antibodies that are specifically immunoreactive with
seminal basic protein and not with other proteins, except for
polymorphic variants and alleles of seminal basic protein. This
selection may be achieved by subtracting out antibodies that
cross-react with seminal basic protein molecules from other
species. A variety of immunoassay formats may be used to select
antibodies specifically immunoreactive with a particular protein.
For example, solid-phase ELISA immunoassays are routinely used to
select antibodies specifically immunoreactive with a protein (see,
e.g., Harlow & Lane, Antibodies, A Laboratory Manual (1988),
for a description of immunoassay formats and conditions that can be
used to determine specific immunoreactivity). Typically a specific
or selective reaction will be at least twice background signal or
noise and more typically more than 10 to 100 times background.
[0071] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00004 Transcript
Name T10888_PEA.sub.--1_T1 (SEQ ID NO: 1) T10888_PEA.sub.--1_T4
(SEQ ID NO: 2) T10888_PEA.sub.--1_T5 (SEQ ID NO: 3)
T10888_PEA.sub.--1_T6 (SEQ ID NO: 4)
[0072] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00005 Segment Name T10888_PEA.sub.--1_node.sub.--11
(SEQ ID NO: 5) T10888_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 6)
T10888_PEA.sub.--1_node.sub.--17 (SEQ ID NO: 7)
T10888_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 8)
T10888_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 9)
T10888_PEA.sub.--1_node.sub.--7 (SEQ ID NO: 10)
T10888_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 11)
T10888_PEA.sub.--1_node.sub.--15 (SEQ ID NO: 12)
[0073] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below amino acid sequence comprising a
sequence in the table below: TABLE-US-00006 Protein Name
T10888_PEA.sub.--1_P2 (SEQ ID NO: 14) T10888_PEA.sub.--1_P4 (SEQ ID
NO: 15) T10888_PEA.sub.--1_P5 (SEQ ID NO: 16) T10888_PEA.sub.--1_P6
(SEQ ID NO: 17)
[0074] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00007 Transcript
Name T39971_T10 (SEQ ID NO: 18) T39971_T12 (SEQ ID NO: 19)
T39971_T16 (SEQ ID NO: 20) T39971_T5 (SEQ ID NO: 21)
[0075] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00008 Segment Name T39971_node.sub.--0 (SEQ ID NO:
22) T39971_node.sub.--18 (SEQ ID NO: 23) T39971_node.sub.--21 (SEQ
ID NO: 24) T39971_node.sub.--22 (SEQ ID NO: 25)
T39971_node.sub.--23 (SEQ ID NO: 26) T39971_node.sub.--31 (SEQ ID
NO: 27) T39971_node.sub.--33 (SEQ ID NO: 28) T39971_node.sub.--7
(SEQ ID NO: 29) T39971_node.sub.--1 (SEQ ID NO: 30)
T39971_node.sub.--10 (SEQ ID NO: 31) T39971_node.sub.--11 (SEQ ID
NO: 32) T39971_node.sub.--12 (SEQ ID NO: 33) T39971_node.sub.--15
(SEQ ID NO: 34) T39971_node.sub.--16 (SEQ ID NO: 35)
T39971_node.sub.--17 (SEQ ID NO: 36) T39971_node.sub.--26 (SEQ ID
NO: 37) T39971_node.sub.--27 (SEQ ID NO: 38) T39971_node.sub.--28
(SEQ ID NO: 39) T39971_node.sub.--29 (SEQ ID NO: 40)
T39971_node.sub.--3 (SEQ ID NO: 41) T39971_node.sub.--30 (SEQ ID
NO: 42) T39971_node.sub.--34 (SEQ ID NO: 43) T39971_node.sub.--35
(SEQ ID NO: 44) T39971_node.sub.--36 (SEQ ID NO: 45)
T39971_node.sub.--4 (SEQ ID NO: 46) T39971_node.sub.--5 (SEQ ID NO:
47) T39971_node.sub.--8 (SEQ ID NO: 48) T39971_node.sub.--9 (SEQ ID
NO: 49)
[0076] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below: TABLE-US-00009 Protein Name T39971_P6
(SEQ ID NO: 51) T39971_P9 (SEQ ID NO: 52) T39971_P11 (SEQ ID NO:
53) T39971_P12 (SEQ ID NO: 54)
[0077] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00010 Transcript
Name Z21368_PEA.sub.--1_T10 (SEQ ID NO: 55) Z21368_PEA.sub.--1_T11
(SEQ ID NO: 56) Z21368_PEA.sub.--1_T23 (SEQ ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ ID NO: 58) Z21368_PEA.sub.--1_T5 (SEQ
ID NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID NO: 61)
[0078] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00011 Segment Name Z21368_PEA.sub.--1_node.sub.--0
(SEQ ID NO: 62) Z21368_PEA.sub.--1_node.sub.--15 (SEQ ID NO: 63)
Z21368_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 64)
Z21368_PEA.sub.--1_node.sub.--2 (SEQ ID NO: 65)
Z21368_PEA.sub.--1_node.sub.--21 (SEQ ID NO: 66)
Z21368_PEA.sub.--1_node.sub.--33 (SEQ ID NO: 67)
Z21368_PEA.sub.--1_node.sub.--36 (SEQ ID NO: 68)
Z21368_PEA.sub.--1_node.sub.--37 (SEQ ID NO: 69)
Z21368_PEA.sub.--1_node.sub.--39 (SEQ ID NO: 70)
Z21368_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 71)
Z21368_PEA.sub.--1_node.sub.--41 (SEQ ID NO: 72)
Z21368_PEA.sub.--1_node.sub.--43 (SEQ ID NO: 73)
Z21368_PEA.sub.--1_node.sub.--45 (SEQ ID NO: 74)
Z21368_PEA.sub.--1_node.sub.--53 (SEQ ID NO: 75)
Z21368_PEA.sub.--1_node.sub.--56 (SEQ ID NO: 76)
Z21368_PEA.sub.--1_node.sub.--58 (SEQ ID NO: 77)
Z21368_PEA.sub.--1_node.sub.--66 (SEQ ID NO: 78)
Z21368_PEA.sub.--1_node.sub.--67 (SEQ ID NO: 79)
Z21368_PEA.sub.--1_node 69 (SEQ ID NO: 80)
Z21368_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 81)
Z21368_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 82)
Z21368_PEA.sub.--1_node.sub.--16 (SEQ ID NO: 83)
Z21368_PEA.sub.--1_node.sub.--17 (SEQ ID NO: 84)
Z21368_PEA.sub.--1_node.sub.--23 (SEQ ID NO: 85)
Z21368_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 86)
Z21368_PEA.sub.--1_node.sub.--30 (SEQ ID NO: 87)
Z21368_PEA.sub.--1_node.sub.--31 (SEQ ID NO: 88)
Z21368_PEA.sub.--1_node.sub.--38 (SEQ ID NO: 89)
Z21368_PEA.sub.--1_node 47 (SEQ ID NO: 90)
Z21368_PEA.sub.--1_node.sub.--49 (SEQ ID NO: 91)
Z21368_PEA.sub.--1_node.sub.--51 (SEQ ID NO: 92)
Z21368_PEA.sub.--1_node.sub.--61 (SEQ ID NO: 93)
Z21368_PEA.sub.--1_node.sub.--68 (SEQ ID NO: 94)
Z21368_PEA.sub.--1_node.sub.--7 (SEQ ID NO: 95)
[0079] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00012 Protein Name
Z21368_PEA.sub.--1_P2 (SEQ ID NO: 97) Z21368_PEA.sub.--1_P5 (SEQ ID
NO: 98) Z21368_PEA.sub.--1_P15 (SEQ ID NO: 99)
Z21368_PEA.sub.--1_P16 (SEQ ID NO: 100) Z21368_PEA.sub.--1_P22 (SEQ
ID NO: 101) Z21368_PEA.sub.--1_P23 (SEQ ID NO: 102)
[0080] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00013 Transcript
Name T59832_T11 (SEQ ID NO: 103) T59832_T15 (SEQ ID NO: 104)
T59832_T22 (SEQ ID NO: 105) T59832_T28 (SEQ ID NO: 106) T59832_T6
(SEQ ID NO: 107) T59832_T8 (SEQ ID NO: 108)
[0081] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00014 Segment Name T59832_node.sub.--1 (SEQ ID NO:
109) T59832_node.sub.--22 (SEQ ID NO: 110) T59832_node.sub.--23
(SEQ ID NO: 111) T59832_node.sub.--24 (SEQ ID NO: 112)
T59832_node.sub.--29 (SEQ ID NO: 113) T59832_node.sub.--39 (SEQ ID
NO: 114) T59832_node.sub.--7 (SEQ ID NO: 115) T59832_node.sub.--10
(SEQ ID NO: 116) T59832_node.sub.--11 (SEQ ID NO: 117)
T59832_node.sub.--12 (SEQ ID NO: 118) T59832_node.sub.--14 (SEQ ID
NO: 119) T59832_node.sub.--16 (SEQ ID NO: 120) T59832_node.sub.--19
(SEQ ID NO: 121) T59832_node.sub.--2 (SEQ ID NO: 122)
T59832_node.sub.--20 (SEQ ID NO: 123) T59832_node.sub.--25 (SEQ ID
NO: 124) T59832_node.sub.--26 (SEQ ID NO: 125) T59832_node.sub.--27
(SEQ ID NO: 126) T59832_node.sub.--28 (SEQ ID NO: 127)
T59832_node.sub.--3 (SEQ ID NO: 128) T59832_node.sub.--30 (SEQ ID
NO: 129) T59832_node.sub.--31 (SEQ ID NO: 130) T59832_node.sub.--32
(SEQ ID NO: 131) T59832_node.sub.--34 (SEQ ID NO: 132)
T59832_node.sub.--35 (SEQ ID NO: 133) T59832_node.sub.--36 (SEQ ID
NO: 134) T59832_node.sub.--37 (SEQ ID NO: 135) T59832_node.sub.--38
(SEQ ID NO: 136) T59832_node.sub.--4 (SEQ ID NO: 137)
T59832_node.sub.--5 (SEQ ID NO: 138) T59832_node.sub.--6 (SEQ ID
NO: 139) T59832_node.sub.--8 (SEQ ID NO: 140) T59832_node.sub.--9
(SEQ ID NO: 141)
[0082] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00015 Protein Name T59832_P5
(SEQ ID NO: 143) T59832_P7 (SEQ ID NO: 144) T59832_P9 (SEQ ID NO:
145) T59832_P12 (SEQ ID NO: 146) T59832_P18 (SEQ ID NO: 147)
[0083] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00016 Transcript
Name Z41644_PEA.sub.--1_T5 (SEQ ID NO: 208)
[0084] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00017 Segment Name Z41644_PEA.sub.--1_node.sub.--0
(SEQ ID NO: 209) Z41644_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 210)
Z41644_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 211)
Z41644_PEA.sub.--1_node.sub.--15 (SEQ ID NO: 212)
Z41644_PEA.sub.--1_node.sub.--20 (SEQ ID NO: 213)
Z41644_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 214)
Z41644_PEA.sub.--1_node.sub.--1 (SEQ ID NO: 215)
Z41644_PEA.sub.--1_node.sub.--10 (SEQ ID NO: 216)
Z41644_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 217)
Z41644_PEA.sub.--1_node.sub.--16 (SEQ ID NO: 218)
Z41644_PEA.sub.--1_node.sub.--17 (SEQ ID NO: 219)
Z41644_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 220)
Z41644_PEA.sub.--1_node.sub.--2 (SEQ ID NO: 221)
Z41644_PEA.sub.--1_node.sub.--21 (SEQ ID NO: 222)
Z41644_PEA.sub.--1_node.sub.--22 (SEQ ID NO: 223)
Z41644_PEA.sub.--1_node.sub.--23 (SEQ ID NO: 224)
Z41644_PEA.sub.--1_node.sub.--25 (SEQ ID NO: 225)
Z41644_PEA.sub.--1_node.sub.--3 (SEQ ID NO: 226)
Z41644_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 227)
Z41644_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 228)
Z41644_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 229)
[0085] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00018 Protein Name
Z41644_PEA.sub.--1_P10 (SEQ ID NO: 231)
[0086] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00019 Transcript
Name HUMGRP5E_T4 (SEQ ID NO:148) HUMGRP5E_T5 (SEQ ID NO:149)
[0087] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00020 Segment Name HUMGRP5E_node.sub.--0 (SEQ ID
NO:150) HUMGRP5E_node.sub.--2 (SEQ ID NO:151) HUMGRP5E_node.sub.--8
(SEQ ID NO:152) HUMGRP5E_node.sub.--3 (SEQ ID NO:153)
HUMGRP5E_node.sub.--7 (SEQ ID NO:154)
[0088] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00021 Protein Name HUMGRP5E_P4
(SEQ ID NO:156) HUMGRP5E_P5 (SEQ ID NO:157)
[0089] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00022 Transcript
Name AA155578_PEA.sub.--1_T10 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 (SEQ ID NO: 159) AA155578_PEA.sub.--1_T13
(SEQ ID NO: 160) AA155578_PEA.sub.--1_T8 (SEQ ID NO: 161)
[0090] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00023 Segment Name
AA155578_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 162)
AA155578_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 163)
AA155578_PEA.sub.--1_node.sub.--14 (SEQ ID NO: 164)
AA155578_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 165)
AA155578_PEA.sub.--1_node.sub.--21 (SEQ ID NO: 166)
AA155578_PEA.sub.--1_node.sub.--23 (SEQ ID NO: 167)
AA155578_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 168)
AA155578_PEA.sub.--1_node.sub.--25 (SEQ ID NO: 169)
AA155578_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 170)
AA155578_PEA.sub.--1_node.sub.--7 (SEQ ID NO: 171)
AA155578_PEA.sub.--1_node.sub.--15 (SEQ ID NO: 172)
AA155578_PEA.sub.--1_node.sub.--18 (SEQ ID NO: 173)
AA155578_PEA.sub.--1_node.sub.--22 (SEQ ID NO: 174)
AA155578_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 175)
AA155578_PEA.sub.--1_node.sub.--8 (SEQ ID NO: 176)
[0091] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00024 Protein Name
AA155578_PEA.sub.--1_P4 (SEQ ID NO: 178) AA155578_PEA.sub.--1_P6
(SEQ ID NO: 179) AA155578_PEA.sub.--1_P8 (SEQ ID NO: 180)
AA155578_PEA.sub.--1_P9 (SEQ ID NO: 181)
[0092] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00025 Transcript
Name HSENA78_T5 (SEQ ID NO:182)
[0093] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00026 Segment Name HSENA78_node.sub.--0 (SEQ ID
NO:183) HSENA78_node.sub.--2 (SEQ ID NO:184) HSENA78_node.sub.--6
(SEQ ID NO:185) HSENA78_node.sub.--9 (SEQ ID NO:186)
HSENA78_node.sub.--3 (SEQ ID NO:187) HSENA78_node.sub.--4 (SEQ ID
NO:188) HSENA78_node.sub.--8 (SEQ ID NO:189)
[0094] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below: TABLE-US-00027 Protein Name HSENA78_P2
(SEQ ID NO:191)
[0095] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00028 Transcript
Name T94936_PEA.sub.--1_T1 (SEQ ID NO: 192) T94936_PEA.sub.--1_T2
(SEQ ID NO: 193)
[0096] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00029 Segment Name T94936_PEA.sub.--1_node.sub.--14
(SEQ ID NO: 194) T94936_PEA.sub.--1_node.sub.--16 (SEQ ID NO: 195)
T94936_PEA.sub.--1_node.sub.--2 (SEQ ID NO: 196)
T94936_PEA.sub.--1_node.sub.--20 (SEQ ID NO: 197)
T94936_PEA.sub.--1_node.sub.--23 (SEQ ID NO: 198)
T94936_PEA.sub.--1_node.sub.--0 (SEQ ID NO: 199)
T94936_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 200)
T94936_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 201)
T94936_PEA.sub.--1_node.sub.--17 (SEQ ID NO: 202)
T94936_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 203)
T94936_PEA.sub.--1_node.sub.--8 (SEQ ID NO: 204)
T94936_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 205)
[0097] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00030 Protein Name
T94936_PEA.sub.--1_P2 (SEQ ID NO: 206) T94936_PEA.sub.--1_P3 (SEQ
ID NO: 207)
[0098] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00031 Transcript
Name M85491_PEA.sub.--1_T16 (SEQ ID NO: 232) M85491_PEA.sub.--1_T20
(SEQ ID NO: 233)
[0099] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00032 Segment Name M85491_PEA.sub.--1_node.sub.--0
(SEQ ID NO: 234) M85491_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 235)
M85491_PEA.sub.--1_node.sub.--21 (SEQ ID NO: 236)
M85491_PEA.sub.--1_node.sub.--23 (SEQ ID NO: 237)
M85491_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 238)
M85491_PEA.sub.--1_node.sub.--8 (SEQ ID NO: 239)
M85491_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 240)
M85491_PEA.sub.--1_node.sub.--10 (SEQ ID NO: 241)
M85491_PEA.sub.--1_node.sub.--18 (SEQ ID NO: 242)
M85491_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 243)
M85491_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 244)
[0100] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00033 Protein Name
M85491_PEA.sub.--1_P13 (SEQ ID NO: 246) M85491_PEA.sub.--1_P14 (SEQ
ID NO: 247)
[0101] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00034 Transcript
Name HSSTROL3_T5 (SEQ ID NO:248) HSSTROL3_T8 (SEQ ID NO:249)
HSSTROL3_T9 (SEQ ID NO:250) HSSTROL3_T10 (SEQ ID NO:251)
HSSTROL3_T11 (SEQ ID NO:252) HSSTROL3_T12 (SEQ ID NO:253)
[0102] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00035 Segment Name HSSTROL3_node.sub.--6 (SEQ ID
NO:254) HSSTROL3_node.sub.--10 (SEQ ID NO:255)
HSSTROL3_node.sub.--13 (SEQ ID NO:256) HSSTROL3_node.sub.--15 (SEQ
ID NO:257) HSSTROL3_node.sub.--19 (SEQ ID NO:258)
HSSTROL3_node.sub.--21 (SEQ ID NO:259) HSSTROL3_node.sub.--24 (SEQ
ID NO:260) HSSTROL3_node.sub.--25 (SEQ ID NO:261)
HSSTROL3_node.sub.--26 (SEQ ID NO:262) HSSTROL3_node.sub.--28 (SEQ
ID NO:263) HSSTROL3_node.sub.--29 (SEQ ID NO:264)
HSSTROL3_node.sub.--11 (SEQ ID NO:265) HSSTROL3_node.sub.--17 (SEQ
ID NO:266) HSSTROL3_node.sub.--18 (SEQ ID NO:267)
HSSTROL3_node.sub.--20 (SEQ ID NO:268) HSSTROL3_node.sub.--27 (SEQ
ID NO:269)
[0103] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00036 Protein Name HSSTROL3_P4
(SEQ ID NO:271) HSSTROL3_P5 (SEQ ID NO:272) HSSTROL3_P7 (SEQ ID
NO:273) HSSTROL3_P8 (SEQ ID NO:274) HSSTROL3_P9 (SEQ ID NO:275)
[0104] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00037 Transcript
Name AY180924_PEA.sub.--1_T1 (SEQ ID NO: 276)
[0105] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00038 Segment Name
AY180924_PEA.sub.--1_node.sub.--3 (SEQ ID NO: 277)
AY180924_PEA.sub.--1_node.sub.--0 (SEQ ID NO: 278)
AY180924_PEA.sub.--1_node.sub.--2 (SEQ ID NO: 279)
[0106] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00039 Protein Name
AY180924_PEA.sub.--1_P3 (SEQ ID NO: 281)
[0107] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00040 Transcript
Name R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) R75793_PEA.sub.--1_T3
(SEQ ID NO: 283) R75793_PEA.sub.--1_T5 (SEQ ID NO: 284)
[0108] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00041 R75793_PEA.sub.--1_node.sub.--0 (SEQ ID NO:
285) R75793_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 286)
R75793_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 287)
R75793_PEA.sub.--1_node.sub.--14 (SEQ ID NO: 288)
R75793_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 289)
R75793_PEA.sub.--1_node.sub.--5 (SEQ ID NO: 290)
R75793_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 291)
R75793_PEA.sub.--1_node.sub.--8 (SEQ ID NO: 292)
R75793_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 293)
[0109] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00042 Protein Name
R75793_PEA.sub.--1_P2 (SEQ ID NO: 295) R75793_PEA.sub.--1_P5 (SEQ
ID NO: 296) R75793_PEA.sub.--1_P6 (SEQ ID NO: 297)
[0110] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00043 Transcript
Name HUMCA1XIA_T16 (SEQ ID NO:298) HUMCA1XIA_T17 (SEQ ID NO:299)
HUMCA1XIA_T19 (SEQ ID NO:300) HUMCA1XIA_T20 (SEQ ID NO:301)
[0111] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00044 Segment Name HUMCA1XIA_node.sub.--0 (SEQ ID
NO:302) HUMCA1XIA_node.sub.--2 (SEQ ID NO:303)
HUMCA1XIA_node.sub.--4 (SEQ ID NO:304) HUMCA1XIA_node.sub.--6 (SEQ
ID NO:305) HUMCA1XIA_node.sub.--8 (SEQ ID NO:306)
HUMCA1XIA_node.sub.--9 (SEQ ID NO:307) HUMCA1XIA_node.sub.--18 (SEQ
ID NO:308) HUMCA1XIA_node.sub.--54 (SEQ ID NO:309)
HUMCA1XIA_node.sub.--55 (SEQ ID NO:310) HUMCA1XIA_node.sub.--92
(SEQ ID NO:311) HUMCA1XIA_node.sub.--11 (SEQ ID NO:312)
HUMCA1XIA_node.sub.--15 (SEQ ID NO:313) HUMCA1XIA_node.sub.--19
(SEQ ID NO:314) HUMCA1XIA_node.sub.--21 (SEQ ID NO:315)
HUMCA1XIA_node.sub.--23 (SEQ ID NO:316) HUMCA1XIA_node.sub.--25
(SEQ ID NO:317) HUMCA1XIA_node.sub.--27 (SEQ ID NO:318)
HUMCA1XIA_node.sub.--29 (SEQ ID NO:319) HUMCA1XIA_node.sub.--31
(SEQ ID NO:320) HUMCA1XIA_node.sub.--33 (SEQ ID NO:321)
HUMCA1XIA_node.sub.--35 (SEQ ID NO:322) HUMCA1XIA_node.sub.--37
(SEQ ID NO:323) HUMCA1XIA_node.sub.--39 (SEQ ID NO:324)
HUMCA1XIA_node.sub.--41 (SEQ ID NO:325) HUMCA1XIA_node.sub.--43
(SEQ ID NO:326) HUMCA1XIA_node.sub.--45 (SEQ ID NO:327)
HUMCA1XIA_node.sub.--47 (SEQ ID NO:328) HUMCA1XIA_node.sub.--49
(SEQ ID NO:329) HUMCA1XIA_node.sub.--51 (SEQ ID NO:330)
HUMCA1XIA_node.sub.--57 (SEQ ID NO:331) HUMCA1XIA_node.sub.--59
(SEQ ID NO:332) HUMCA1XIA_node.sub.--62 (SEQ ID NO:333)
HUMCA1XIA_node.sub.--64 (SEQ ID NO:334) HUMCA1XIA_node.sub.--66
(SEQ ID NO:335) HUMCA1XIA_node.sub.--68 (SEQ ID NO:336)
HUMCA1XIA_node.sub.--70 (SEQ ID NO:337) HUMCA1XIA_node.sub.--72
(SEQ ID NO:338) HUMCA1XIA_node.sub.--74 (SEQ ID NO:339)
HUMCA1XIA_node.sub.--76 (SEQ ID NO:340) HUMCA1XIA_node.sub.--78
(SEQ ID NO:341) HUMCA1XIA_node.sub.--81 (SEQ ID NO:342)
HUMCA1XIA_node.sub.--83 (SEQ ID NO:343) HUMCA1XIA_node.sub.--85
(SEQ ID NO:344) HUMCA1XIA_node.sub.--87 (SEQ ID NO:345)
HUMCA1XIA_node.sub.--89 (SEQ ID NO:346) HUMCA1XIA_node.sub.--91
(SEQ ID NO:347)
[0112] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00045 Protein Name
HUMCA1XIA_P14 (SEQ ID NO:350) HUMCA1XIA_P15 (SEQ ID NO:351)
HUMCA1XIA_P16 (SEQ ID NO:352) HUMCA1XIA_P17 (SEQ ID NO:353)
[0113] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00046 Transcript
Name R20779_T7 (SEQ ID NO: 354)
[0114] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00047 Segment Name R20779_node.sub.--0 (SEQ ID NO:
355) R20779_node.sub.--2 (SEQ ID NO: 356) R20779_node.sub.--7 (SEQ
ID NO: 357) R20779_node.sub.--9 (SEQ ID NO: 358)
R20779_node.sub.--18 (SEQ ID NO: 359) R20779_node.sub.--21 (SEQ ID
NO: 360) R20779_node.sub.--24 (SEQ ID NO: 361) R20779_node.sub.--27
(SEQ ID NO: 362) R20779_node.sub.--28 (SEQ ID NO: 363)
R20779_node.sub.--30 (SEQ ID NO: 364) R20779_node.sub.--31 (SEQ ID
NO: 365) R20779_node.sub.--32 (SEQ ID NO: 366) R20779_node.sub.--1
(SEQ ID NO: 367) R20779_node.sub.--3 (SEQ ID NO: 368)
R20779_node.sub.--10 (SEQ ID NO: 369) R20779_node.sub.--11 (SEQ ID
NO: 370) R20779_node.sub.--14 (SEQ ID NO: 371) R20779_node.sub.--17
(SEQ ID NO: 372) R20779_node.sub.--19 (SEQ ID NO: 373)
R20779_node.sub.--20 (SEQ ID NO: 374) R20779_node.sub.--22 (SEQ ID
NO: 375) R20779_node 23 (SEQ ID NO: 376) R20779_node.sub.--25 (SEQ
ID NO: 377) R20779_node.sub.--29 (SEQ ID NO: 378)
[0115] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence according to R20779_P2 (SEQ ID NO:380).
[0116] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00048 Transcript
Name HSS100PCB_T1 (SEQ ID NO:381)
[0117] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00049 Segment Name HSS100PCB_node.sub.--3 (SEQ ID
NO:382) HSS100PCB_node.sub.--4 (SEQ ID NO:383)
HSS100PCB_node.sub.--5 (SEQ ID NO:384)
[0118] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence according to HSS100PCB_P3 (SEQ ID NO:386).
[0119] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00050 Transcript
Name HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387) HSCOC4_PEA.sub.--1_T2
(SEQ ID NO:388) HSCOC4_PEA.sub.--1_T3 (SEQ ID NO:389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390) HSCOC4_PEA.sub.--1_T5 (SEQ ID
NO:391) HSCOC4_PEA.sub.--1_T7 (SEQ ID NO:392) HSCOC4_PEA.sub.--1_T8
(SEQ ID NO:393) HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395) HSCOC4_PEA.sub.--1_T14 (SEQ
ID NO:396) HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398) HSCOC4_PEA.sub.--1_T21 (SEQ
ID NO:399) HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401) HSCOC4_PEA.sub.--1_T30 (SEQ
ID NO:402) HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404) HSCOC4_PEA.sub.--1_T40 (SEQ
ID NO:405)
[0120] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00051 Segment Name HSCOC4_PEA.sub.--1_node.sub.--1
(SEQ ID NO:406) HSCOC4_PEA.sub.--1_node.sub.--5 (SEQ ID NO:407)
HSCOC4_PEA.sub.--1_node.sub.--7 (SEQ ID NO:408)
HSCOC4_PEA.sub.--1_node.sub.--30 (SEQ ID NO:409)
HSCOC4_PEA.sub.--1_node.sub.--33 (SEQ ID NO:410)
HSCOC4_PEA.sub.--1_node.sub.--35 (SEQ ID NO:411)
HSCOC4_PEA.sub.--1_node.sub.--37 (SEQ ID NO:412)
HSCOC4_PEA.sub.--1_node.sub.--39 (SEQ ID NO:413)
HSCOC4_PEA.sub.--1_node.sub.--43 (SEQ ID NO:414)
HSCOC4_PEA.sub.--1_node.sub.--48 (SEQ ID NO:415)
HSCOC4_PEA.sub.--1_node.sub.--49 (SEQ ID NO:416)
HSCOC4_PEA.sub.--1_node.sub.--51 (SEQ ID NO:417)
HSCOC4_PEA.sub.--1_node.sub.--58 (SEQ ID NO:418)
HSCOC4_PEA.sub.--1_node.sub.--59 (SEQ ID NO:419)
HSCOC4_PEA.sub.--1_node.sub.--62 (SEQ ID NO:420)
HSCOC4_PEA.sub.--1_node.sub.--66 (SEQ ID NO:421)
HSCOC4_PEA.sub.--1_node.sub.--72 (SEQ ID NO:422)
HSCOC4_PEA.sub.--1_node.sub.--77 (SEQ ID NO:423)
HSCOC4_PEA.sub.--1_node.sub.--79 (SEQ ID NO:424)
HSCOC4_PEA.sub.--1_node.sub.--93 (SEQ ID NO:425)
HSCOC4_PEA.sub.--1_node.sub.--100 (SEQ ID NO:426)
HSCOC4_PEA.sub.--1_node.sub.--105 (SEQ ID NO:427)
HSCOC4_PEA.sub.--1_node.sub.--107 (SEQ ID NO:428)
HSCOC4_PEA.sub.--1_node.sub.--108 (SEQ ID NO:429)
HSCOC4_PEA.sub.--1_node.sub.--109 (SEQ ID NO:430)
HSCOC4_PEA.sub.--1_node.sub.--110 (SEQ ID NO:431)
HSCOC4_PEA.sub.--1_node.sub.--112 (SEQ ID NO:432)
HSCOC4_PEA.sub.--1_node.sub.--113 (SEQ ID NO:433)
HSCOC4_PEA.sub.--1_node.sub.--2 (SEQ ID NO:434)
HSCOC4_PEA.sub.--1_node.sub.--8 (SEQ ID NO:435)
HSCOC4_PEA.sub.--1_node.sub.--10 (SEQ ID NO:436)
HSCOC4_PEA.sub.--1_node.sub.--12 (SEQ ID NO:437)
HSCOC4_PEA.sub.--1_node.sub.--14 (SEQ ID NO:438)
HSCOC4_PEA.sub.--1_node.sub.--17 (SEQ ID NO:439)
HSCOC4_PEA.sub.--1_node.sub.--19 (SEQ ID NO:440)
HSCOC4_PEA.sub.--1_node.sub.--21 (SEQ ID NO:441)
HSCOC4_PEA.sub.--1_node.sub.--22 (SEQ ID NO:442)
HSCOC4_PEA.sub.--1_node.sub.--28 (SEQ ID NO:443)
HSCOC4_PEA.sub.--1_node.sub.--29 (SEQ ID NO:444)
HSCOC4_PEA.sub.--1_node.sub.--41 (SEQ ID NO:445)
HSCOC4_PEA.sub.--1_node.sub.--45 (SEQ ID NO:446)
HSCOC4_PEA.sub.--1_node.sub.--47 (SEQ ID NO:447)
HSCOC4_PEA.sub.--1_node.sub.--50 (SEQ ID NO:448)
HSCOC4_PEA.sub.--1_node.sub.--53 (SEQ ID NO:449)
HSCOC4_PEA.sub.--1_node.sub.--55 (SEQ ID NO:450)
HSCOC4_PEA.sub.--1_node.sub.--57 (SEQ ID NO:451)
HSCOC4_PEA.sub.--1_node.sub.--60 (SEQ ID NO:452)
HSCOC4_PEA.sub.--1_node.sub.--64 (SEQ ID NO:453)
HSCOC4_PEA.sub.--1_node.sub.--69 (SEQ ID NO:454)
HSCOC4_PEA.sub.--1_node.sub.--70 (SEQ ID NO:455)
HSCOC4_PEA.sub.--1_node.sub.--71 (SEQ ID NO:456)
HSCOC4_PEA.sub.--1_node.sub.--73 (SEQ ID NO:457)
HSCOC4_PEA.sub.--1_node.sub.--74 (SEQ ID NO:458)
HSCOC4_PEA.sub.--1_node.sub.--75 (SEQ ID NO:459)
HSCOC4_PEA.sub.--1_node.sub.--76 (SEQ ID NO:460)
HSCOC4_PEA.sub.--1_node.sub.--78 (SEQ ID NO:461)
HSCOC4_PEA.sub.--1_node.sub.--80 (SEQ ID NO:462)
HSCOC4_PEA.sub.--1_node.sub.--82 (SEQ ID NO:463)
HSCOC4_PEA.sub.--1_node.sub.--83 (SEQ ID NO:464)
HSCOC4_PEA.sub.--1_node.sub.--84 (SEQ ID NO:465)
HSCOC4_PEA.sub.--1_node.sub.--85 (SEQ ID NO:466)
HSCOC4_PEA.sub.--1_node.sub.--86 (SEQ ID NO:467)
HSCOC4_PEA.sub.--1_node.sub.--87 (SEQ ID NO:468)
HSCOC4_PEA.sub.--1_node.sub.--88 (SEQ ID NO:469)
HSCOC4_PEA.sub.--1_node.sub.--89 (SEQ ID NO:470)
HSCOC4_PEA.sub.--1_node.sub.--90 (SEQ ID NO:471)
HSCOC4_PEA.sub.--1_node.sub.--91 (SEQ ID NO:472)
HSCOC4_PEA.sub.--1_node.sub.--92 (SEQ ID NO:473)
HSCOC4_PEA.sub.--1_node.sub.--94 (SEQ ID NO:474)
HSCOC4_PEA.sub.--1_node.sub.--96 (SEQ ID NO:475)
HSCOC4_PEA.sub.--1_node.sub.--97 (SEQ ID NO:476)
HSCOC4_PEA.sub.--1_node.sub.--98 (SEQ ID NO:477)
HSCOC4_PEA.sub.--1_node.sub.--99 (SEQ ID NO:478)
HSCOC4_PEA.sub.--1_node.sub.--101 (SEQ ID NO:479)
HSCOC4_PEA.sub.--1_node.sub.--102 (SEQ ID NO:480)
HSCOC4_PEA.sub.--1_node.sub.--103 (SEQ ID NO:481)
HSCOC4_PEA.sub.--1_node.sub.--104 (SEQ ID NO:482)
HSCOC4_PEA.sub.--1_node.sub.--106 (SEQ ID NO:483)
HSCOC4_PEA.sub.--1_node.sub.--111 (SEQ ID NO:484)
[0121] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00052 Protein Name
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488) HSCOC4_PEA.sub.--1_P5 (SEQ ID
NO:489) HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490)
HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491) HSCOC4_PEA.sub.--1_P15 (SEQ
ID NO:492) HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493)
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494) HSCOC4_PEA.sub.--1_P9 (SEQ
ID NO:495) HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496)
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497) HSCOC4_PEA.sub.--1_P24 (SEQ
ID NO:498) HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499)
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500) HSCOC4_PEA.sub.--1_P30 (SEQ
ID NO:501) HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502)
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503) HSCOC4_PEA.sub.--1_P40 (SEQ
ID NO:504) HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505)
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506)
[0122] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00053 Transcript
Name HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508)
[0123] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00054 Segment Name
HUMTREFAC_PEA.sub.--2_node.sub.--0 (SEQ ID NO:509)
HUMTREFAC_PEA.sub.--2_node.sub.--9 (SEQ ID NO:510)
HUMTREFAC_PEA.sub.--2_node.sub.--2 (SEQ ID NO:511)
HUMTREFAC_PEA.sub.--2_node.sub.--3 (SEQ ID NO:512)
HUMTREFAC_PEA.sub.--2_node.sub.--4 (SEQ ID NO:513)
HUMTREFAC_PEA.sub.--2_node.sub.--5 (SEQ ID NO:514)
HUMTREFAC_PEA.sub.--2_node.sub.--8 (SEQ ID NO:515)
[0124] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00055 Protein Name
HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:517) HUMTREFAC_PEA.sub.--2_P8
(SEQ ID NO:518)
[0125] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00056 Transcript
Name HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521)
[0126] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00057 Segment Name
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--0 (SEQ ID NO:522)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--10 (SEQ ID NO:523)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--16 (SEQ ID NO:524)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--23 (SEQ ID NO:525)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--31 (SEQ ID NO:526)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--43 (SEQ ID NO:527)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--3 (SEQ ID NO:528)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--5 (SEQ ID NO:529)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--7 (SEQ ID NO:530)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--8 (SEQ ID NO:531)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--15 (SEQ ID NO:532)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--17 (SEQ ID NO:533)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--20 (SEQ ID NO:534)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--21 (SEQ ID NO:535)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--22 (SEQ ID NO:536)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--24 (SEQ ID NO:537)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--26 (SEQ ID NO:538)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--27 (SEQ ID NO:539)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--28 (SEQ ID NO:540)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--29 (SEQ ID NO:541)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--30 (SEQ ID NO:542)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--32 (SEQ ID NO:543)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--34 (SEQ ID NO:544)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--36 (SEQ ID NO:545)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--37 (SEQ ID NO:546)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--38 (SEQ ID NO:547)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--39 (SEQ ID NO:548)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--40 (SEQ ID NO:549)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--41 (SEQ ID NO:550)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--42 (SEQ ID NO:551)
[0127] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00058 Protein Name
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555)
[0128] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a
polynucleotide having a sequence selected from the group consisting
of: R11723_PEA.sub.--1_T15 (SEQ ID NO:556), R11723_PEA.sub.--1_T17
(SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ ID NO:558),
R11723_PEA.sub.--1_T20 (SEQ ID NO:559), R11723_PEA.sub.--1_T5 (SEQ
ID NO:560), or R11723_PEA.sub.--1_T6 (SEQ ID NO:561).
[0129] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a node
having a sequence selected from the group consisting of:
R11723_PEA.sub.--1_node.sub.--13 (SEQ ID NO:562),
R11723_PEA.sub.--1_node.sub.--16 (SEQ ID NO:563),
R11723_PEA.sub.--1_node.sub.--19 (SEQ ID NO:564),
R11723_PEA.sub.--1_node.sub.--2 (SEQ ID NO:565),
R11723_PEA.sub.--1_node.sub.--22 (SEQ ID NO:566),
R11723_PEA.sub.--1_node.sub.--31 (SEQ ID NO:567),
R11723_PEA.sub.--1_node.sub.--10 (SEQ ID NO:568),
R11723_PEA.sub.--1_node.sub.--11 (SEQ ID NO:569),
R11723_PEA.sub.--1_node.sub.--15 (SEQ ID NO:570),
R11723_PEA.sub.--1_node.sub.--18 (SEQ ID NO:571),
R11723_PEA.sub.--1_node.sub.--20 (SEQ ID NO:572),
R11723_PEA.sub.--1_node.sub.--21 (SEQ ID NO:573),
R11723_PEA.sub.--1_node.sub.--23 (SEQ ID NO:574),
R11723_PEA.sub.--1_node.sub.--24 (SEQ ID NO:575),
R11723_PEA.sub.--1_node.sub.--25 (SEQ ID NO:576),
R11723_PEA.sub.--1_node.sub.--26 (SEQ ID NO:577),
R11723_PEA.sub.--1_node.sub.--27 (SEQ ID NO:578),
R11723_PEA.sub.--1_node.sub.--28 (SEQ ID NO:579),
R11723_PEA.sub.--1_node.sub.--29 (SEQ ID NO:580),
R11723_PEA.sub.--1_node.sub.--3 (SEQ ID NO:581),
R11723_PEA.sub.--1_node.sub.--30 (SEQ ID NO:582),
R11723_PEA.sub.--1_node.sub.--4 (SEQ ID NO:583),
R11723_PEA.sub.--1_node.sub.--5 (SEQ ID NO:584),
R11723_PEA.sub.--1_node.sub.--6 (SEQ ID NO:585),
R11723_PEA.sub.--1_node.sub.--7 (SEQ ID NO:586) or
R11723_PEA.sub.--1_node.sub.--8 (SEQ ID NO:587).
[0130] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising a polypeptide
having a sequence selected from the group consisting of:
R11723_PEA.sub.--1.sub.13 P2 (SEQ ID NO:588), R11723_PEA.sub.--1_P6
(SEQ ID NO:589), R11723_PEA.sub.--1_P7 (SEQ ID NO:590),
R11723_PEA.sub.--1_P13 (SEQ ID NO:591), or R11723_PEA.sub.--1_P10
(SEQ ID NO:592).
[0131] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00059 Transcript
Name T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) T46984_PEA.sub.--1_T3
(SEQ ID NO: 594) T46984_PEA.sub.--1.sub.--1_T2 (SEQ ID NO: 595)
T46984_PEA.sub.--1_T3 (SEQ ID NO: 596) T46984_PEA.sub.--1_T4 (SEQ
ID NO: 597) T46984_PEA.sub.--1_T15 (SEQ ID NO: 598)
T46984_PEA.sub.--1_T9 (SEQ ID NO: 599) T46984_PEA.sub.--1_T23 (SEQ
ID NO: 600) T46984_PEA.sub.--1_T27 (SEQ ID NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID NO: 602) T46984_PEA.sub.--1_T34 (SEQ
ID NO: 603) T46984_PEA.sub.--1_T35 (SEQ ID NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID NO: 605) T46984_PEA.sub.--1_T42 (SEQ
ID NO: 606) T46984_PEA.sub.--1_T43 (SEQ ID NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID NO: 608) T46984_PEA.sub.--1_T47 (SEQ
ID NO: 609) T46984_PEA.sub.--1_T48 (SEQ ID NO: 610)
T46984_PEA.sub.--1_T51 (SEQ ID NO: 611) T46984_PEA.sub.--1_T52 (SEQ
ID NO: 612) T46984_PEA.sub.--1_T54 (SEQ ID NO: 613)
[0132] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00060 Segment Name T46984_PEA.sub.--1_node.sub.--2
(SEQ ID NO: 614) T46984_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 615)
T46984_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 616)
T46984_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 617)
T46984_PEA.sub.--1_node.sub.--14 (SEQ ID NO: 618)
T46984_PEA.sub.--1_node.sub.--25 (SEQ ID NO: 619)
T46984_PEA.sub.--1_node.sub.--29 (SEQ ID NO: 620)
T46984_PEA.sub.--1_node.sub.--34 (SEQ ID NO: 621)
T46984_PEA.sub.--1_node.sub.--46 (SEQ ID NO: 622)
T46984_PEA.sub.--1_node.sub.--47 (SEQ ID NO: 623)
T46984_PEA.sub.--1_node.sub.--52 (SEQ ID NO: 624)
T46984_PEA.sub.--1_node.sub.--65 (SEQ ID NO: 625)
T46984_PEA.sub.--1_node.sub.--69 (SEQ ID NO: 626)
T46984_PEA.sub.--1_node.sub.--75 (SEQ ID NO: 627)
T46984_PEA.sub.--1_node.sub.--86 (SEQ ID NO: 628)
T46984_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 629)
T46984_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 630)
T46984_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 631)
T46984_PEA.sub.--1_node.sub.--21 (SEQ ID NO: 632)
T46984_PEA.sub.--1_node.sub.--22 (SEQ ID NO: 633)
T46984_PEA.sub.--1_node.sub.--26 (SEQ ID NO: 634)
T46984_PEA.sub.--1_node.sub.--28 (SEQ ID NO: 635)
T46984_PEA.sub.--1_node.sub.--31 (SEQ ID NO: 636)
T46984_PEA.sub.--1_node.sub.--32 (SEQ ID NO: 637)
T46984_PEA.sub.--1_node.sub.--38 (SEQ ID NO: 638)
T46984_PEA.sub.--1_node.sub.--39 (SEQ ID NO: 639)
T46984_PEA.sub.--1_node.sub.--40 (SEQ ID NO: 640)
T46984_PEA.sub.--1_node.sub.--42 (SEQ ID NO: 641)
T46984_PEA.sub.--1_node.sub.--43 (SEQ ID NO: 642)
T46984_PEA.sub.--1_node.sub.--48 (SEQ ID NO: 643)
T46984_PEA.sub.--1_node.sub.--49 (SEQ ID NO: 644)
T46984_PEA.sub.--1_node.sub.--50 (SEQ ID NO: 645)
T46984_PEA.sub.--1_node.sub.--51 (SEQ ID NO: 646)
T46984_PEA.sub.--1_node.sub.--53 (SEQ ID NO: 647)
T46984_PEA.sub.--1_node.sub.--54 (SEQ ID NO: 648)
T46984_PEA.sub.--1_node.sub.--55 (SEQ ID NO: 649)
T46984_PEA.sub.--1_node.sub.--57 (SEQ ID NO: 650) T46984
PEA.sub.--1_node 60 (SEQ ID NO: 651)
T46984_PEA.sub.--1_node.sub.--62 (SEQ ID NO: 652)
T46984_PEA.sub.--1_node.sub.--66 (SEQ ID NO: 653)
T46984_PEA.sub.--1_node.sub.--67 (SEQ ID NO: 654)
T46984_PEA.sub.--1_node.sub.--70 (SEQ ID NO: 655)
T46984_PEA.sub.--1_node.sub.--71 (SEQ ID NO: 656)
T46984_PEA.sub.--1_node.sub.--72 (SEQ ID NO: 657)
T46984_PEA.sub.--1_node.sub.--73 (SEQ ID NO: 658)
T46984_PEA.sub.--1_node.sub.--74 (SEQ ID NO: 659)
T46984_PEA.sub.--1_node.sub.--83 (SEQ ID NO: 660)
T46984_PEA.sub.--1_node.sub.--84 (SEQ ID NO: 661)
T46984_PEA.sub.--1_node.sub.--85 (SEQ ID NO: 662)
[0133] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00061 Protein Name
T46984_PEA.sub.--1_P2 (SEQ ID NO: 664) T46984_PEA.sub.--1_P3 (SEQ
ID NO: 665) T46984_PEA.sub.--1_P10 (SEQ ID NO: 666) T46984_PEA
1_P11 (SEQ ID NO: 667) T46984_PEA.sub.--1_P12 (SEQ ID NO: 668)
T46984_PEA.sub.--1_P21 (SEQ ID NO: 669) T46984_PEA.sub.--1_P27 (SEQ
ID NO: 670) T46984_PEA.sub.--1_P32 (SEQ ID NO: 671)
T46984_PEA.sub.--1_P34 (SEQ ID NO: 672) T46984_PEA.sub.--1_P35 (SEQ
ID NO: 673) T46984_PEA.sub.--1_P38 (SEQ ID NO: 674)
T46984_PEA.sub.--1_P39 (SEQ ID NO: 675) T46984_PEA.sub.--1_P45 (SEQ
ID NO: 676) T46984_PEA.sub.--1_P46 (SEQ ID NO: 677)
[0134] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00062 Transcript
Name T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) T11628_PEA.sub.--1_T4
(SEQ ID NO: 679) T11628_PEA.sub.--1_T5 (SEQ ID NO: 680)
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) T11628_PEA.sub.--1_T9 (SEQ
ID NO: 682) T11628_PEA.sub.--1_T11 (SEQ ID NO: 683)
[0135] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00063 Segment Name T11628_PEA.sub.--1_node.sub.--7
(SEQ ID NO: 684) T11628_PEA.sub.--1_node.sub.--11 (SEQ ID NO: 685)
T11628_PEA.sub.--1_node.sub.--16 (SEQ ID NO: 686)
T11628_PEA.sub.--1_node.sub.--22 (SEQ ID NO: 687)
T11628_PEA.sub.--1_node.sub.--25 (SEQ ID NO: 688)
T11628_PEA.sub.--1_node.sub.--31 (SEQ ID NO: 689)
T11628_PEA.sub.--1_node.sub.--37 (SEQ ID NO: 690)
T11628_PEA.sub.--1_node.sub.--0 (SEQ ID NO: 691)
T11628_PEA.sub.--1_node.sub.--4 (SEQ ID NO: 692)
T11628_PEA.sub.--1_node.sub.--9 (SEQ ID NO: 693)
T11628_PEA.sub.--1_node.sub.--13 (SEQ ID NO: 694)
T11628_PEA.sub.--1_node.sub.--14 (SEQ ID NO: 695)
T11628_PEA.sub.--1_node.sub.--17 (SEQ ID NO: 696)
T11628_PEA.sub.--1_node.sub.--18 (SEQ ID NO: 697)
T11628_PEA.sub.--1_node.sub.--19 (SEQ ID NO: 698)
T11628_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 699)
T11628_PEA.sub.--1_node.sub.--27 (SEQ ID NO: 700)
T11628_PEA.sub.--1_node.sub.--28 (SEQ ID NO: 701)
T11628_PEA.sub.--1_node.sub.--29 (SEQ ID NO: 702)
T11628_PEA.sub.--1_node.sub.--30 (SEQ ID NO: 703)
T11628_PEA.sub.--1_node.sub.--32 (SEQ ID NO: 704)
T11628_PEA.sub.--1_node.sub.--33 (SEQ ID NO: 705)
T11628_PEA.sub.--1_node.sub.--34 (SEQ ID NO: 706)
T11628_PEA.sub.--1_node.sub.--35 (SEQ ID NO: 707)
T11628_PEA.sub.--1_node.sub.--36 (SEQ ID NO: 708)
[0136] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00064 Protein Name
T11628_PEA.sub.--1_P2 (SEQ ID NO: 712) T11628_PEA.sub.--1_P5 (SEQ
ID NO: 713) T11628_PEA.sub.--1_P7 (SEQ ID NO: 714)
T11628_PEA.sub.--1_P10 (SEQ ID NO: 715)
[0137] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00065 Transcript
Name M78076_PEA.sub.--1_T2 (SEQ ID NO: 716) M78076_PEA.sub.--1_T3
(SEQ ID NO: 717) M78076_PEA.sub.--1_T5 (SEQ ID NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID NO: 719) M78076_PEA.sub.--1_T15 (SEQ
ID NO: 720) M78076_PEA.sub.--1_T23 (SEQ ID NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID NO: 722) M78076_PEA.sub.--1_T27 (SEQ
ID NO: 723) M78076_PEA.sub.--1_T28 (SEQ ID NO: 724)
[0138] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00066 Segment Name M78076_PEA.sub.--1_node.sub.--0
(SEQ ID NO: 725) M78076_PEA.sub.--1_node.sub.--10 (SEQ ID NO: 726)
M78076_PEA.sub.--1_node.sub.--15 (SEQ ID NO: 727)
M78076_PEA.sub.--1_node.sub.--18 (SEQ ID NO: 728)
M78076_PEA.sub.--1_node.sub.--20 (SEQ ID NO: 729)
M78076_PEA.sub.--1_node.sub.--24 (SEQ ID NO: 730)
M78076_PEA.sub.--1_node.sub.--26 (SEQ ID NO: 731)
M78076_PEA.sub.--1_node.sub.--29 (SEQ ID NO: 732)
M78076_PEA.sub.--1_node.sub.--32 (SEQ ID NO: 733)
M78076_PEA.sub.--1_node.sub.--35 (SEQ ID NO: 734)
M78076_PEA.sub.--1_node.sub.--37 (SEQ ID NO: 735)
M78076_PEA.sub.--1_node.sub.--46 (SEQ ID NO: 736)
M78076_PEA.sub.--1_node.sub.--47 (SEQ ID NO: 737)
M78076_PEA.sub.--1_node.sub.--54 (SEQ ID NO: 738)
M78076_PEA.sub.--1_node.sub.--1 (SEQ ID NO: 739)
M78076_PEA.sub.--1_node.sub.--2 (SEQ ID NO: 740)
M78076_PEA.sub.--1_node.sub.--3 (SEQ ID NO: 741)
M78076_PEA.sub.--1_node.sub.--6 (SEQ ID NO: 742)
M78076_PEA.sub.--1_node.sub.--7 (SEQ ID NO: 743)
M78076_PEA.sub.--1_node.sub.--12 (SEQ ID NO: 744)
M78076_PEA.sub.--1_node.sub.--22 (SEQ ID NO: 745)
M78076_PEA.sub.--1_node.sub.--27 (SEQ ID NO: 746)
M78076_PEA.sub.--1_node.sub.--30 (SEQ ID NO: 747)
M78076_PEA.sub.--1_node.sub.--31 (SEQ ID NO: 748)
M78076_PEA.sub.--1_node.sub.--34 (SEQ ID NO: 749)
M78076_PEA.sub.--1_node.sub.--36 (SEQ ID NO: 750)
M78076_PEA.sub.--1_node.sub.--41 (SEQ ID NO: 751)
M78076_PEA.sub.--1_node.sub.--42 (SEQ ID NO: 752)
M78076_PEA.sub.--1_node.sub.--43 (SEQ ID NO: 753)
M78076_PEA.sub.--1_node.sub.--45 (SEQ ID NO: 754)
M78076_PEA.sub.--1_node.sub.--49 (SEQ ID NO: 755)
M78076_PEA.sub.--1_node.sub.--50 (SEQ ID NO: 756)
M78076_PEA.sub.--1_node.sub.--51 (SEQ ID NO: 757)
M78076_PEA.sub.--1_node.sub.--52 (SEQ ID NO: 758)
M78076_PEA.sub.--1_node.sub.--53 (SEQ ID NO: 759)
[0139] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00067 Protein Name
M78076_PEA.sub.--1_P3 (SEQ ID NO: 761) M78076_PEA.sub.--1_P4 (SEQ
ID NO: 762) M78076_PEA.sub.--1_P12 (SEQ ID NO: 763)
M78076_PEA.sub.--1_P14 (SEQ ID NO: 764) M78076_PEA.sub.--1_P21 (SEQ
ID NO: 765) M78076_PEA.sub.--1_P24 (SEQ ID NO: 766)
M78076_PEA.sub.--1_P2 (SEQ ID NO: 767) M78076_PEA.sub.--1_P25 (SEQ
ID NO: 768)
[0140] According to preferred embodiments of the present invention,
there is provided an isolated polynucleotide comprising a nucleic
acid sequence in the table below and/or: TABLE-US-00068 Transcript
Name HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769)
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770) HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771) HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773) HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774) HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776) HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777) HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779) HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780) HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781)
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782)
[0141] a nucleic acid sequence comprising a sequence in the table
below: TABLE-US-00069 Segment Name HSMUC1A_PEA.sub.--1_node.sub.--0
(SEQ ID NO:783) HSMUC1A_PEA.sub.--1_node.sub.--14 (SEQ ID NO:784)
HSMUC1A_PEA.sub.--1_node.sub.--24 (SEQ ID NO:785)
HSMUC1A_PEA.sub.--1_node.sub.--29 (SEQ ID NO:786)
HSMUC1A_PEA.sub.--1_node.sub.--35 (SEQ ID NO:787)
HSMUC1A_PEA.sub.--1_node.sub.--38 (SEQ ID NO:788)
HSMUC1A_PEA.sub.--1_node.sub.--3 (SEQ ID NO:789)
HSMUC1A_PEA.sub.--1_node.sub.--4 (SEQ ID NO:790)
HSMUC1A_PEA.sub.--1_node.sub.--5 (SEQ ID NO:791)
HSMUC1A_PEA.sub.--1_node.sub.--6 (SEQ ID NO:792)
HSMUC1A_PEA.sub.--1_node.sub.--7 (SEQ ID NO:793)
HSMUC1A_PEA.sub.--1_node.sub.--17 (SEQ ID NO:794)
HSMUC1A_PEA.sub.--1_node.sub.--18 (SEQ ID NO:795)
HSMUC1A_PEA.sub.--1_node.sub.--20 (SEQ ID NO:796)
HSMUC1A_PEA.sub.--1_node.sub.--21 (SEQ ID NO:797)
HSMUC1A_PEA.sub.--1_node.sub.--23 (SEQ ID NO:798)
HSMUC1A_PEA.sub.--1_node.sub.--26 (SEQ ID NO:799)
HSMUC1A_PEA.sub.--1_node.sub.--27 (SEQ ID NO:800)
HSMUC1A_PEA.sub.--1_node.sub.--31 (SEQ ID NO:801)
HSMUC1A_PEA.sub.--1_node.sub.--34 (SEQ ID NO:802)
HSMUC1A_PEA.sub.--1_node.sub.--36 (SEQ ID NO:803)
HSMUC1A_PEA.sub.--1_node.sub.--37 (SEQ ID NO:804)
[0142] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide comprising an amino acid
sequence in the table below TABLE-US-00070 Protein Name
HSMUC1A_PEA.sub.--1_P25 (SEQ ID NO:806) HSMUC1A_PEA.sub.--1_P29
(SEQ ID NO:807) HSMUC1A_PEA.sub.--1_P30 (SEQ ID NO:808)
HSMUC1A_PEA.sub.--1_P32 (SEQ ID NO:809) HSMUC1A_PEA.sub.--1_P36
(SEQ ID NO:810) HSMUC1A_PEA.sub.--1_P39 (SEQ ID NO:811)
HSMUC1A_PEA.sub.--1_P45 (SEQ ID NO:812) HSMUC1A_PEA.sub.--1_P49
(SEQ ID NO:813) HSMUC1A_PEA.sub.--1_P52 (SEQ ID NO:814)
HSMUC1A_PEA.sub.--1_P53 (SEQ ID NO:815) HSMUC1A_PEA.sub.--1_P56
(SEQ ID NO:816) HSMUC1A_PEA.sub.--1_P58 (SEQ ID NO:817)
HSMUC1A_PEA.sub.--1_P59 (SEQ ID NO:818) HSMUC1A_PEA.sub.--1_P63
(SEQ ID NO:819)
[0143] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819), comprising a first amino
acid sequence being at least 90% homologous to
MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSV corresponding to
amino acids 1-45 of MUC1_HUMAN (SEQ ID NO:805), which also
corresponds to amino acids 1-45 of HSMUC1A_PEA.sub.--1_P63 (SEQ ID
NO:819), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO:946)
corresponding to amino acids 46-85 of HSMUC1A_PEA.sub.--1_P63 (SEQ
ID NO:819), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[0144] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO:946) in
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819).
[0145] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P2 (SEQ ID NO:664), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLMVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-498 of T46984_PEA.sub.--1_P2 (SEQ ID NO:664), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VCA corresponding to amino acids 499-501 of
T46984_PEA.sub.--1_P2 (SEQ ID NO:664), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0146] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQ corresponding to amino acids 1-433 of RIB2_HUMAN
(SEQ ID NO:663), which also corresponds to amino acids 1-433 of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ICHIWKLIFLP (SEQ ID NO:947) corresponding to amino acids 434-444 of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0147] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ICHIWKLIFLP (SEQ ID NO:947) in T46984_PEA.sub.--1_P3 (SEQ ID
NO:665).
[0148] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P10 (SEQ ID NO:666), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-498 of T46984_PEA.sub.--1_P10 (SEQ ID NO:666), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence LMDQK (SEQ ID NO:948) corresponding to amino acids 499-503
of T46984_PEA.sub.--1_P10 (SEQ ID NO:666), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0149] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P10 (SEQ ID NO:666), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence LMDQK (SEQ
ID NO:948) in T46984_PEA.sub.--1_P10 (SEQ ID NO:666).
[0150] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P11 (SEQ ID NO:667), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNVADWIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEK
RPPTWSNTFTALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWT
QLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR corresponding to amino acids
1-628 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds to
amino acids 1-628 of T46984_PEA.sub.--1_P11 (SEQ ID NO:667).
[0151] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P12 (SEQ ID NO:668), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMN corresponding to amino
acids 1-338 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds
to amino acids 1-338 of T46984_PEA.sub.--1_P12 (SEQ ID NO:668), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence SQDLH (SEQ ID NO:949) corresponding to amino acids
339-343 of T46984_PEA.sub.--1_P12 (SEQ ID NO:668), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0152] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P12 (SEQ ID NO:668), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence SQDLH (SEQ
ID NO:949) in T46984_PEA.sub.--1_P12 (SEQ ID NO:668).
[0153] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P21 (SEQ ID NO:669), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence M corresponding to amino acids 1-1 of
T46984_PEA.sub.--1_P21 (SEQ ID NO:669), and a second amino acid
sequence being at least 90% homologous to
KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAV
AALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA
RLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFES
LSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKL
EHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVEL
RVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNT
GAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLII
GDATLKNPILWNVADWIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTF
TALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKY
LAILGSVTFLAGNRMLAQQAVKRTAH corresponding to amino acids 70-631 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
2-563 of T46984_PEA.sub.--1_P21 (SEQ ID NO:669), wherein said first
amino acid sequence and second amino acid sequence are contiguous
and in a sequential order.
[0154] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P27 (SEQ ID NO:670), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFA
corresponding to amino acids 1-415 of RIB2_HUMAN (SEQ ID NO:663),
which also corresponds to amino acids 1-415 of
T46984_PEA.sub.--1.sub.P27 (SEQ ID NO:670), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO:950)
corresponding to amino acids 416-459 of T46984_PEA.sub.--1_P27 (SEQ
ID NO:670), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[0155] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P27 (SEQ ID NO:670), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO:950) in
T46984_PEA.sub.--1_P27 (SEQ ID NO:670).
[0156] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVE
corresponding to amino acids 1-364 of RIB2_HUMAN (SEQ ID NO:663),
which also corresponds to amino acids 1-364 of
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO:951) corresponding to
amino acids 365-397 of T46984_PEA.sub.--1_P32 (SEQ ID NO:671),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0157] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO:951) in
T46984_PEA.sub.--1_P32 (SEQ ID NO:671).
[0158] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P34 (SEQ ID NO:672), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPWWPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVG corresponding to amino acids 1-329
of RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino
acids 1-329 of T46984_PEA.sub.--1.sub.13 P34 (SEQ ID NO:672).
[0159] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P35 (SEQ ID NO:673), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLMVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI corresponding to
amino acids 1-287 of RIB2_HUMAN (SEQ ID NO:663), which also
corresponds to amino acids 1-287 of T46984_PEA.sub.--1_P35 (SEQ ID
NO:673), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO:952)
corresponding to amino acids 288-334 of T46984_PEA.sub.--1_P35 (SEQ
ID NO:673), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[0160] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P35 (SEQ ID NO:673), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO:952) in
T46984_PEA.sub.--1_P35 (SEQ ID NO:673).
[0161] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P38 (SEQ ID NO:674), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEAL corresponding to amino acids 1-145 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-145 of T46984_PEA.sub.--1_P38 (SEQ ID NO:674), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MDPDWCQCLQLHFCS (SEQ ID NO:953) corresponding to amino
acids 146-160 of T46984_PEA.sub.--1_P38 (SEQ ID NO:674), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0162] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P38 (SEQ ID NO:674), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
MDPDWCQCLQLHFCS (SEQ ID NO:953) in T46984_PEA.sub.--1_P38 (SEQ ID
NO:674)
[0163] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P39 (SEQ ID NO:675), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLA corresponding to amino acids
1-160 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds to
amino acids 1-160 of T46984_PEA.sub.--1_P39 (SEQ ID NO:675).
[0164] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P45 (SEQ ID NO:676), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYMQASQALSGCE corresponding to amino
acids 1-101 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds
to amino acids 1-101 of T46984_PEA.sub.--1_P45 (SEQ ID NO:676), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence NSPGSADSIPPVPAG (SEQ ID NO:954) corresponding to amino
acids 102-116 of T46984_PEA.sub.--1_P45 (SEQ ID NO:676), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0165] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P45 (SEQ ID NO:676), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) in T46984_PEA.sub.--1_P45 (SEQ ID
NO:676).
[0166] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAK corresponding to amino acids 1-69 of RIB2_HUMAN (SEQ ID
NO:663), which also corresponds to amino acids 1-69 of
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) corresponding to amino acids 70-84
of T46984_PEA.sub.--1_P46 (SEQ ID NO:677), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0167] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) in T46984_PEA.sub.--1_P46 (SEQ ID
NO:677).
[0168] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P2 (SEQ ID NO:712), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE
(SEQ ID NO:956) corresponding to amino acids 1-55 of
T11628_PEA.sub.--1_P2 (SEQ ID NO:712), and a second amino acid
sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 1-99 of Q8WVH6 (SEQ ID NO:711), which also corresponds to
amino acids 56-154 of T11628_PEA.sub.--1_P2 (SEQ ID NO:712),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0169] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
T11628_PEA.sub.--1_P2 (SEQ ID NO:712), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00071
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE (SEQ ID
NO:956) of T11628_PEA.sub.--1_P2 (SEQ ID NO:712)
[0170] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P5 (SEQ ID NO:713), comprising a first amino
acid sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 56-154 of MYG_HUMAN_V1 (SEQ ID NO:710), which also
corresponds to amino acids 1-99 of T11628_PEA.sub.--1_P5 (SEQ ID
NO:713).
[0171] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P7 (SEQ ID NO:714), comprising a first amino
acid sequence being at least 90% homologous to
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMK
ASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQ
SKHPGDFGADAQGAMNK corresponding to amino acids 1-134 of
MYG_HUMAN_V1 (SEQ ID NO:710), which also corresponds to amino acids
1-134 of T11628_PEA.sub.--1_P7 (SEQ ID NO:714) and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence G corresponding to amino acids 135-135 of
T11628_PEA.sub.--1_P7 (SEQ ID NO:714), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0172] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P10 (SEQ ID NO:715), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE
(SEQ ID NO:956) corresponding to amino acids 1-55 of
T111628_PEA.sub.--1_P10 (SEQ ID NO:715), and a second amino acid
sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 1-99 of Q8WVH6 (SEQ ID NO:711), which also corresponds to
amino acids 56-154 of T11628_PEA.sub.--1_P10 (SEQ ID NO:715),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0173] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
T11628_PEA.sub.--1_P10 (SEQ ID NO:715), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE (SEQ ID
NO:956) of T11628_PEA.sub.--1_P10 (SEQ ID NO:715).
[0174] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P3 (SEQ ID NO:761), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLMLQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKD corresponding to
amino acids 1-517 of APP1_HUMAN (SEQ ID NO:760), which also
corresponds to amino acids 1-517 of M78076_PEA.sub.--1_P3 (SEQ ID
NO:761), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence GE corresponding to amino acids
518-519 of M78076_PEA.sub.--1_P3 (SEQ ID NO:761), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0175] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGMEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKG
corresponding to amino acids 1-526 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-526 of
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ECLTVNPSLQIPLNP (SEQ ID NO:958) corresponding to amino acids
527-541 of M78076_PEA.sub.--1_P4 (SEQ ID NO:762), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0176] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ECLTVNPSLQIPLNP (SEQ ID NO:958) in M78076_PEA.sub.--1_P4 (SEQ ID
NO:762).
[0177] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGMEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKG
corresponding to amino acids 1-526 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-526 of
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ECVCSKGFPFPLIGDSEG (SEQ ID NO:959) corresponding to amino acids
527-544 of M78076_PEA.sub.--1_P12 (SEQ ID NO:763), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0178] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ECVCSKGFPFPLIGDSEG (SEQ ID NO:959) in M78076_PEA.sub.--1_P12 (SEQ
ID NO:763).
[0179] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGMEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGST
EQDMSPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDEL corresponding to amino
acids 1-570 of APP1_HUMAN (SEQ ID NO:760), which also corresponds
to amino acids 1-570 of M78076_PEA.sub.--1_P14 (SEQ ID NO:764), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence VRGGTAGYLGEETRGQRPGCDSQSHTGPSKKPSAPSPLPAGTSWDRGVP (SEQ
ID NO:960) corresponding to amino acids 571-619 of
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0180] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VRGGTAGYLGEETRGQRPGCDSQSHTGPSKKPSAPSPLPAGTSWDRGVP (SEQ ID NO:960)
in M78076_PEA.sub.--1_P14 (SEQ ID NO:764).
[0181] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN E
corresponding to amino acids 1-352 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-352 of
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), and a second amino acid
sequence being at least 90% homologous to
AERVLLALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQ
SLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMT
LPKGSTEQDMSPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREA
VSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGWEVDPMLTLEEQQLRELQRHGYE
NPTYRFLEERP corresponding to amino acids 406-650 of APP1_HUMAN (SEQ
ID NO:760), which also corresponds to amino acids 353-597 of
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0182] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of M78076_PEA.sub.--1_P21 (SEQ ID NO:765), comprising
a polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EA, having a structure as follows: a
sequence starting from any of amino acid numbers 352-x to 352; and
ending at any of amino acid numbers 353+((n-2)-x), in which x
varies from 0 to n-2.
[0183] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQI corresponding to amino acids 1-481 of APP1_HUMAN (SEQ
ID NO:760), which also corresponds to amino acids 1-481 of
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
RECLLPWLPLQISEGRS (SEQ ID NO:961) corresponding to amino acids
482-498 of M78076_PEA.sub.--1_P24 (SEQ ID NO:766), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0184] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RECLLPWLPLQISEGRS (SEQ ID NO:961) in M78076_PEA.sub.--1_P24 (SEQ ID
NO:766).
[0185] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P2 (SEQ ID NO:767), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGMEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLMLQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQV corresponding to amino acids
1-449 of APP1_HUMAN (SEQ ID NO:760), which also corresponds to
amino acids 1-449 of M78076_PEA.sub.--1_P2 (SEQ ID NO:767), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence
LTSFQLPNAPLFLRRPRLRLFSCPLDPLSVSWTPSYPLNTASLPLPSLSAQLPDPETWTLT
CCVFDPCFLALGFLLPPPSILCSVPWIFTAFPRIVFFFFFFLRQVLALSPRQESSVRSWLIAT
STSWVQAILLPQPLE (SEQ ID NO:962) corresponding to amino acids
450-588 of M78076_PEA.sub.--1_P2 (SEQ ID NO:767), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0186] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P2 (SEQ ID NO:767), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00072
LTSFQLPNAPLFLRRPRLRLFSCPLDPLSVSWTPSYPLNTASLPLPSLSAQLPDPETWTLT (SEQ
ID NO:962)
CCVFDPCFLALGFLLPPPSILCSVPWIFTAFPRIVFFFFFFLRQVLALSPRQESSVRSWLIAT
STSWVQAILLPQPLE in M78076_PEA.sub.--1_P2 (SEQ ID NO:767)
[0187] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQ corresponding to amino acids
1-448 of APP1_HUMAN (SEQ ID NO:760), which also corresponds to
amino acids 1-448 of M78076_PEA.sub.--1_P25 (SEQ ID NO:768), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence PQNPNSQPRAAGSLEVIISHPFVRRLEILISPFQFQNSIPKNSQIVPAASPRGTSSP
(SEQ ID NO:963) corresponding to amino acids 449-505 of
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0188] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
PQNPNSQPRAAGSLEVIISHPFVRRLEILISPFQFQNSIPKNSQIVPAASPRGTSSP (SEQ ID
NO:963) in M78076_PEA.sub.--1_P25 (SEQ ID NO:768).
[0189] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M85491_PEA.sub.--1_P13 (SEQ ID NO:246), comprising a first amino
acid sequence being at least 90% homologous to
MALRRLGMLLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIR
TYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYY
EADFDSATKTFPNWMENPWVKVDTIMDESFSQVDLGGRVMKINTEVRSFGPVSRSGF
YLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVMRGSCIANAEEVD
VPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPIN
SRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSG
GREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAV
NGVTDQSPFSPQFASVNITTNQMPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYEL QYYEK
corresponding to amino acids 1-476 of EPB2_HUMAN (SEQ ID NO:245),
which also corresponds to amino acids 1-476 of
M85491_PEA.sub.--1_P13 (SEQ ID NO:246), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VPIGWVLSPSPTSLRAPLPG (SEQ ID NO:964) corresponding to amino acids
477-496 of M85491_PEA.sub.--1_P13 (SEQ ID NO:246), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0190] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M85491_PEA.sub.--1_P13 (SEQ ID NO:246), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VPIGWVLSPSPTSLRAPLPG (SEQ ID NO:964) in M85491_PEA.sub.--1_P13 (SEQ
ID NO:246).
[0191] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
M85491_PEA.sub.--1_P14 (SEQ ID NO:247), comprising a first amino
acid sequence being at least 90% homologous to
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIR
TYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYY
EADFDSATKTFPNWMENPWVKVDTIMDESFSQVDLGGRVMKINTEVRSFGPVSRSGF
YLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVMRGSCIANAEEVD
VPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR corresponding to amino acids
1-270 of EPB2_HUMAN (SEQ ID NO:245), which also corresponds to
amino acids 1-270 of M85491_PEA.sub.--1_P14 (SEQ ID NO:247), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence ERQDLTMLSRLVLNSWPQMILPPQPPKVLEL (SEQ ID NO:965)
corresponding to amino acids 271-301 of M85491_PEA.sub.--1_P14 (SEQ
ID NO:247), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0192] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M85491_PEA.sub.--1_P14 (SEQ ID NO:247), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ERQDLTMLSRLVLNSWPQMILPPQPPKVLEL (SEQ ID NO:965) in
M85491_PEA.sub.--1_P14 (SEQ ID NO:247).
[0193] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSSTROL3_P4 (SEQ ID NO:271), comprising a first amino acid sequence
being at least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHMLPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P4 (SEQ ID NO:271), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P4 (SEQ ID NO:271), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVMHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDMFEDAQGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPE
KNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDMFQDADG corresponding to
amino acids 165-445 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 165-445 of HSSTROL3_P4 (SEQ ID NO:271),
and a third amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence ALGVRQLVGGGHSSRFSHLWAGLPHACHRKSGSSSQVLCPEPSALLSVAG
(SEQ ID NO:966) corresponding to amino acids 446-496 of HSSTROL3_P4
(SEQ ID NO:271), wherein said first amino acid sequence, bridging
amino acid, second amino acid sequence and third amino acid
sequence are contiguous and in a sequential order.
[0194] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSSTROL3_P4 (SEQ ID NO:271), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence
ALGVRQLVGGGHSSRFSHLWAGLPHACHRKSGSSSQVLCPEPSALLSVAG (SEQ ID NO:966)
in HSSTROL3_P4 (SEQ ID NO:271).
[0195] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSSTROL.sup.3_P5 (SEQ ID NO:272), comprising a first amino acid
sequence being at least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHMLPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P5 (SEQ ID NO:272), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P5 (SEQ ID NO:272), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVMHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDMFEDAQGHIWFFQ corresponding to amino acids 165-358 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
165-358 of HSSTROL3_P5 (SEQ ID NO:272), and a third amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ELGFPSSTGRDESLEHCRCQGLHK (SEQ ID NO:967) corresponding to amino
acids 359-382 of HSSTROL3_P5 (SEQ ID NO:272), wherein said first
amino acid sequence, bridging amino acid, second amino acid
sequence and third amino acid sequence are contiguous and in a
sequential order.
[0196] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSSTROL3_P5 (SEQ ID NO:272), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence ELGFPSSTGRDESLEHCRCQGLHK
(SEQ ID NO:967) in HSSTROL3_P5 (SEQ ID NO:272).
[0197] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSSTROL3_P7 (SEQ ID NO:273), comprising a first amino acid sequence
being at least 90% homologous to
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHMLPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P7 (SEQ ID NO:273), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P7 (SEQ ID NO:273), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDAAFEDAQGHIWFFQG corresponding to amino acids 165-359 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
165-359 of HSSTROL3_P7 (SEQ ID NO:273), and a third amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TTGVSTPAPGV (SEQ ID NO:968) corresponding to amino acids 360-370 of
HSSTROL3_P7 (SEQ ID NO:273), wherein said first amino acid
sequence, bridging amino acid, second amino acid sequence and third
amino acid sequence are contiguous and in a sequential order.
[0198] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSSTROL3_P7 (SEQ ID NO:273), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence TTGVSTPAPGV (SEQ ID
NO:968) in HSSTROL3_P7 (SEQ ID NO:273).
[0199] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSSTROL3_P8 (SEQ ID NO:274), comprising a first amino acid sequence
being at least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P8 (SEQ ID NO:274), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P8 (SEQ ID NO:274), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVMHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN EIAPLE
corresponding to amino acids 165-286 of MM11_HUMAN (SEQ ID NO:270),
which also corresponds to amino acids 165-286 of HSSTROL3_P8 (SEQ
ID NO:274), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence VRPCLPVPLLLCWPL (SEQ ID NO:969)
corresponding to amino acids 287-301 of HSSTROL3_P8 (SEQ ID
NO:274), wherein said first amino acid sequence, bridging amino
acid, second amino acid sequence and third amino acid sequence are
contiguous and in a sequential order.
[0200] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSSTROL3_P8 (SEQ ID NO:274), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence VRPCLPVPLLLCWPL (SEQ ID
NO:969) in HSSTROL3_P8 (SEQ ID NO:274).
[0201] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSSTROL3_P9 (SEQ ID NO:275), comprising a first amino acid sequence
being at least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQK corresponding to amino acids
1-96 of MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino
acids 1-96 of HSSTROL3_P9 (SEQ ID NO:275), a second amino acid
sequence being at least 90% homologous to
RILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding
to amino acids 113-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 97-147 of HSSTROL3_P9 (SEQ ID NO:275), a
bridging amino acid H corresponding to amino acid 148 of
HSSTROL3_P9 (SEQ ID NO:275), a third amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDMFEDAQGHIWFFQG corresponding to amino acids 165-359 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
149-343 of HSSTROL3_P9 (SEQ ID NO:275), and a fourth amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TTGVSTPAPGV (SEQ ID NO:968) corresponding to amino acids 344-354 of
HSSTROL3_P9 (SEQ ID NO:275), wherein said first amino acid
sequence, second amino acid sequence, bridging amino acid, third
amino acid sequence and fourth amino acid sequence are contiguous
and in a sequential order.
[0202] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of HSSTROL3_P9 (SEQ ID NO:275), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise KR, having a structure as follows: a
sequence starting from any of amino acid numbers 96-x to 96; and
ending at any of amino acid numbers 97+((n-2)-x), in which x varies
from 0 to n-2.
[0203] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSSTROL3_P9 (SEQ ID NO:275), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence TTGVSTPAPGV (SEQ ID
NO:968) in HSSTROL3_P9 (SEQ ID NO:275).
[0204] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
AY180924_PEA.sub.--1_P3 (SEQ ID NO:281), comprising a first amino
acid sequence being at least 90% homologous to
MLNVSGLFVLLCGLLVSSSAQEVLAGVSSQLLN corresponding to amino acids 1-33
of LATH_HUMAN (SEQ ID NO:280), which also corresponds to amino
acids 1-33 of AY180924_PEA.sub.--1_P3 (SEQ ID NO:281), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence GETVLLWVMQNPEPMPVKFSLAKYLGHNEHY (SEQ ID NO:971)
corresponding to amino acids 34-64 of AY180924_PEA.sub.--1_P3 (SEQ
ID NO:281), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0205] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
AY180924_PEA.sub.--1_P3 (SEQ ID NO:281), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GETVLLWVMQNPEPMPVKFSLAKYLGHNEHY (SEQ ID NO:971) in
AY180924_PEA.sub.--1_P3 (SEQ ID NO:281).
[0206] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
R75793_PEA.sub.--1_P2 (SEQ ID NO:295), comprising a first amino
acid sequence being at least 90% homologous to
MKFLAVLVLLGVSIFLVSAQNPTTAAPADTYPATGPADDEAPDAETTAAATTATTAAPT
TATTAASTTARKDIP corresponding to amino acids 1-74 of Q96DR8 (SEQ ID
NO:294), which also corresponds to amino acids 1-74 of
R75793_PEA.sub.--1_P2 (SEQ ID NO:295), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence AP
corresponding to amino acids 75-76 of R75793_PEA.sub.--1_P2 (SEQ ID
NO:295), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[0207] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMCA1XIA_P14 (SEQ ID NO:350), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGMPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAG
PRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQG
PIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVK
GADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPT
GDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPR
GQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGP
PGKDGLPGHPGQRGETGFQGKTGPPGPGGWGPQGPTGETGPIGERGHPGPPGPPGEQG
LPGMGKEGAKGDPGPQGISGKDGPAGLRGFPGERGLPGAQGAPGLKGGEGPQGPPGPV
corresponding to amino acids 1-1056 of CA1B_HUMAN_V5 (SEQ ID
NO:349), which also corresponds to amino acids 1-1056 of
HUMCA1XIA_P14 (SEQ ID NO:350), and a second amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence
VSMMIINSQTIMWNYSSSFITLML (SEQ ID NO:972) corresponding to amino
acids 1057-1081 of HUMCA1XIA_P14 (SEQ ID NO:350), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0208] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMCA1XIA_P14 (SEQ ID NO:350), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence VSMMIINSQTIMWNYSSSFITLML
(SEQ ID NO:972) in HUMCA1XIA_P14 (SEQ ID NO:350).
[0209] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMCA1XIA_P15 (SEQ ID NO:351), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGMPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAG
PRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQG PIGPPGEK
corresponding to amino acids 1-714 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 1-714 of HUMCA1XIA_P15 (SEQ
ID NO:351), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence MCCNLSFGILIPLQK (SEQ ID NO:973)
corresponding to amino acids 715-729 of HUMCA1XIA_P15 (SEQ ID
NO:351), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[0210] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMCA1XIA_P15 (SEQ ID NO:351), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence MCCNLSFGILIPLQK (SEQ ID
NO:973) in HUMCA1XIA_P15 (SEQ ID NO:351).
[0211] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMCA1XIA_P16 (SEQ ID NO:352), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEA
corresponding to amino acids 1-648 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 1-648 of HUMCA1XIA_P16 (SEQ
ID NO:352), a second amino acid sequence being at least 90%
homologous to GMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEK
corresponding to amino acids 667-714 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 649-696 of HUMCA1XIA_P16 (SEQ
ID NO:352), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
VSFSFSLFYKKVIKFACDKRFVGRHDERKVVKLSLPLYLIYE (SEQ ID NO:974)
corresponding to amino acids 697-738 of HUMCA1XIA_P16 (SEQ ID
NO:352), wherein said first amino acid sequence, second amino acid
sequence and third amino acid sequence are contiguous and in a
sequential order.
[0212] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of HUMCA1XIA_P16 (SEQ ID NO:352), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise AG, having a structure as follows: a
sequence starting from any of amino acid numbers 648-x to 648; and
ending at any of amino acid numbers 649+((n-2)-x), in which x
varies from 0 to n-2.
[0213] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMCA1XIA_P16 (SEQ ID NO:352), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence
VSFSFSLFYKKVIKFACDKRFVGRHDERKVVKLSLPLYLIYE (SEQ ID NO:974) in
HUMCA1XIA_P16 (SEQ ID NO:352).
[0214] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMCA1XIA_P17 (SEQ ID NO:353), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDE corresponding to amino acids 1-260 of
CA1B_HUMAN (SEQ ID NO:348), which also corresponds to amino acids
1-260 of HUMCA1XIA_P17 (SEQ ID NO:353), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRSTRPEKVFVFQ (SEQ ID NO:975) corresponding to amino acids 261-273
of HUMCA1XIA_P17 (SEQ ID NO:353), wherein said first amino acid
sequence and second amino acid sequence are contiguous and in a
sequential order.
[0215] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMCA1XIA_P17 (SEQ ID NO:353), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence VRSTRPEKVFVFQ (SEQ ID
NO:975) in HUMCA1XIA_P17 (SEQ ID NO:353).
[0216] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
R20779_P2 (SEQ ID NO:380), comprising a first amino acid sequence
being at least 90% homologous to
MCAERLGQFMTLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLV
NAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRH
RFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHE corresponding
to amino acids 1-169 of STC2_HUMAN (SEQ ID NO:379), which also
corresponds to amino acids 1-169 of R20779_P2 (SEQ ID NO:380), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence CYKIEITMPKRRKVKLRD (SEQ ID NO:976) corresponding to
amino acids 170-187 of R20779_P2 (SEQ ID NO:380), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0217] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
R20779_P2 (SEQ ID NO:380), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence CYKIEITMPKRRKVKLRD (SEQ ID
NO:976) in R20779_P2 (SEQ ID NO:380).
[0218] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
DDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTV corresponding to amino acids
1-865 of CO4_HUMAN, which also corresponds to amino acids 1-865 of
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
RPHRSLSIQELGEPGPSEGWGG (SEQ ID NO:977) corresponding to amino acids
866-887 of HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0219] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RPHRSLSIQELGEPGPSEGWGG (SEQ ID NO:977) in HSCOC4_PEA.sub.--1_P3
(SEQ ID NO:488).
[0220] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKG corresponding to
amino acids 1-818 of CO4_HUMAN, which also corresponds to amino
acids 1-818 of HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) corresponding to
amino acids 819-843 of HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0221] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) in HSCOC4_PEA.sub.--1_P5
(SEQ ID NO:489).
[0222] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKG corresponding to
amino acids 1-1052 of CO4_HUMAN, which also corresponds to amino
acids 1-1052 of HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence SGCKGKQEGGQERTVTGRWTAQEATEGKKGGP (SEQ ID NO:979)
corresponding to amino acids 1053-1084 of HSCOC4_PEA.sub.--1_P6
(SEQ ID NO:490), wherein said first amino acid sequence and second
amino acid sequence are contiguous and in a sequential order.
[0223] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SGCKGKQEGGQERTVTGRWTAQEATEGKKGGP (SEQ ID NO:979) in
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490).
[0224] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKV corresponding to amino acids 1-1380
of CO4_HUMAN_V1 (SEQ ID NO:486), which also corresponds to amino
acids 1-1380 of HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence RAREGVGPGTGGGEGVE (SEQ ID NO:980) corresponding to amino
acids 1381-1397 of HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0225] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RAREGVGPGTGGGEGVE (SEQ ID NO:980) in HSCOC4_PEA.sub.--1_P12 (SEQ ID
NO:491).
[0226] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKWARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAMITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQ corresponding to amino acids 1-1359 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1359 of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VNHSLVNHSLAWVARTPGPRGQARSRPQPPTRGIPAALLPGVFGGRLTSWLRDLEL (SEQ ID
NO:981) corresponding to amino acids 1360-1415 of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0227] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VNHSLVNHSLAWVARTPGPRGQARSRPQPPTRGIPAALLPGVFGGRLTSWLRDLEL (SEQ ID
NO:981) in HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492).
[0228] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPK corresponding to
amino acids 1-1457 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1-1457 of HSCOC4_PEA.sub.--1_P16 (SEQ ID
NO:493), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence AERQGGAVWHGHRGRHPPEWIPRPAC (SEQ ID
NO:982) corresponding to amino acids 1458-1483 of
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0229] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
AERQGGAVWHGHRGRHPPEWIPRPAC (SEQ ID NO:982) in
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493).
[0230] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQ corresponding to amino acids 1-1303 of CO4_HUMAN_V1
(SEQ ID NO:486), which also corresponds to amino acids 1-1303 of
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VGAVPGLWRGWWLRPRACLSPGSTSLGHGDCPGCPVCLLDCLPHH (SEQ ID NO:983)
corresponding to amino acids 1304-1349 of HSCOC4_PEA.sub.--1_P20
(SEQ ID NO:494), wherein said first amino acid sequence and second
amino acid sequence are contiguous and in a sequential order.
[0231] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00073 (SEQ ID NO: 983)
VGAVPGLWRGWWLRPRACLSPGSTSLGHGDCPGCPVCLLDCLPHH (SEQ ID NO: 494) in
HSCOC4_PEA.sub.--1_P20.
[0232] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDSV
corresponding to amino acids 1-1529 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1529 of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence SGER (SEQ
ID NO:984) corresponding to amino acids 1530-1533 of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0233] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence SGER (SEQ
ID NO:984) in HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495).
[0234] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAF
RLFETKITQVLHF corresponding to amino acids 1-1653 of CO4_HUMAN_V1
(SEQ ID NO:486), which also corresponds to amino acids 1-1653 of
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SMKQTGEAGRAGGRQGG (SEQ ID NO:985) corresponding to amino acids
1654-1670 of HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0235] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SMKQTGEAGRAGGRQGG (SEQ ID NO:985) in HSCOC4_PEA.sub.--1_P22 (SEQ ID
NO:496).
[0236] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYG corresponding to amino
acids 1-1626 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1-1626 of HSCOC4_PEA.sub.--1_P23 (SEQ ID
NO:497), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
QSSHRGPGLTLPRGPAVLVSLGVACSSYRSCTQPVCSDTNFLPSQPQSNSPFPLLLTPS (SEQ ID
NO:986) corresponding to amino acids 1627-1685 of
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0237] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
QSSHRGPGLTLPRGPAVLVSLGVACSSYRSCTQPVCSDTNFLPSQPQSNSPFPLLLTPS (SEQ ID
NO:986) in HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497).
[0238] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKWARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDS
corresponding to amino acids 1-1528 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1528 of
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SADVLCFTGHQVRADSWPPCVLLKSASVLRGSALASVAPWSGVCRTRMATG (SEQ ID NO:987)
corresponding to amino acids 1529-1579 of HSCOC4_PEA.sub.--1_P24
(SEQ ID NO:498), wherein said first amino acid sequence and second
amino acid sequence are contiguous and in a sequential order.
[0239] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00074 SADVLCFTGHQVRADSWPPCVLLKSASVLRGSALASVAPWSGVCRTRMATG
(SEQ ID NO: 987) in HSCOC4_PEA.sub.--1_P24. (SEQ ID NO: 498)
[0240] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEG corresponding to amino acids 1-1593 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1593 of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDEVCLLLPPC GVRLPG
(SEQ ID NO:988) corresponding to amino acids 1594-1657 of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0241] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00075
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDEVCLLLPPC (SEQ ID
NO: 988) GVRLPG in HSCOC4_PEA.sub.--1_P25. (SEQ ID NO: 499)
[0242] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKWARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEG corresponding to amino acids 1-1593 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1593 of
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TABLE-US-00076
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDEVCLLLPPC (SEQ ID
NO: 989) GVRSVFPPRPWPDPPSGTGCFGLSGCSLLLLQVMHAACLL corresponding to
amino acids 1594-1691 of HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0243] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00077
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDEVCLLLPPC (SEQ ID
NO: 989) GVRSVFPPRPWPDPPSGTGCFGLSGCSLLLLQVMHAACLL in
HSCOC4_PEA.sub.--1_P26. (SEQ ID NO: 500)
[0244] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGS
corresponding to amino acids 1-1232 of CO4_HUMAN_V3 (SEQ ID
NO:487), which also corresponds to amino acids 1-1232 of
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
RNPVRLLQPRAQMFCVLRGTK (SEQ ID NO:990) corresponding to amino acids
1233-1253 of HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0245] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RNPVRLLQPRAQMFCVLRGTK (SEQ ID NO:990) in HSCOC4_PEA.sub.--1_P30
(SEQ ID NO:501).
[0246] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKG corresponding to
amino acids 1-818 of CO4_HUMAN, which also corresponds to amino
acids 1-818 of HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) corresponding to
amino acids 819-843 of HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0247] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) in HSCOC4_PEA.sub.--1_P38
(SEQ ID NO:502).
[0248] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQ corresponding to amino acids 1-387
of CO4_HUMAN, which also corresponds to amino acids 1-387 of
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence VSSRGEG
(SEQ ID NO:992) corresponding to amino acids 388-394 of
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0249] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence VSSRGEG
(SEQ ID NO:992) in HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503).
[0250] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKY
corresponding to amino acids 1-236 of CO4_HUMAN, which also
corresponds to amino acids 1-236 of HSCOC4_PEA.sub.--1_P40 (SEQ ID
NO:504), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence AGEWTEPHFPLKGRVPGRPGEAEYGHY (SEQ ID
NO:993) corresponding to amino acids 237-263 of
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0251] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
AGEWTEPHFPLKGRVPGRPGEAEYGHY (SEQ ID NO:993) in
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504).
[0252] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDSV
corresponding to amino acids 1-1529 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1529 of
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence SGER (SEQ
ID NO:984) corresponding to amino acids 1530-1533 of
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[0253] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1.sub.-1_P41 (SEQ ID NO:505), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
SGER (SEQ ID NO:984) in HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505).
[0254] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV HYTVCIW
corresponding to amino acids 1-1473 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1473 of
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), a second amino acid
sequence being at least 70%, optionally at least-80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
WAPGAALGQGREGRTQAGAGLLEPAQAEPGRQLTRLHR (SEQ ID NO:1021)
corresponding to amino acids 1474-1511 of HSCOC4_PEA.sub.--1_P42
(SEQ ID NO:506), a third amino acid sequence being at least 90%
homologous to RNGKVGLSGMAIADVTLLSGFHALRADLEK corresponding to amino
acids 1474-1503 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1512-1541 of HSCOC4_PEA.sub.--1_P42 (SEQ
ID NO:506), and a fourth amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence VWSATQGNPLCPRY (SEQ ID NO:995)
corresponding to amino acids 1542-1555 of HSCOC4_PEA.sub.--1_P42
(SEQ ID NO:506), wherein said first amino acid sequence, second
amino acid sequence, third amino acid sequence and fourth amino
acid sequence are contiguous and in a sequential order.
[0255] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for an edge
portion of HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), comprising an
amino acid sequence being at least 70%, optionally at least about
80%, preferably at least about 85%, more preferably at least about
90% and most preferably at least about 95% homologous to the
sequence encoding for WAPGAALGQGREGRTQAGAGLLEPAQAEPGRQLTRLHR (SEQ
ID NO: 1021), corresponding to HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506).
[0256] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HSCOC4 PEA.sub.--1_P42 (SEQ ID NO:506), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VWSATQGNPLCPRY (SEQ ID NO:995) in HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506).
[0257] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), comprising a first amino
acid sequence being at least 90% homologous to
MAARALCMLGLVLALLSSSSAEEYVGL corresponding to amino acids 1-27 of
TFF3_HUMAN (SEQ ID NO:516), which also corresponds to amino acids
1-27 of HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence WKVHLPKGEGFSSG (SEQ ID NO:996) corresponding to amino
acids 28-41 of HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0258] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
WKVHLPKGEGFSSG (SEQ ID NO:996) in HUMTREFAC_PEA.sub.--2_P8 (SEQ ID
NO:518).
[0259] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQ
corresponding to amino acids 1-58 of OSTP_HUMAN (SEQ ID NO:552),
which also corresponds to amino acids 1-58 of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VFLNFS (SEQ ID NO:997) corresponding to amino acids 59-64
of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0260] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
VFLNFS (SEQ ID NO:997) in HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ
ID NO:553).
[0261] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31
of OSTP_HUMAN (SEQ ID NO:552), which also corresponds to amino
acids 1-31 of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554),
and a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence H corresponding to amino acids 32-32 of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[0262] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31
of OSTP_HUMAN (SEQ ID NO:552), which also corresponds to amino
acids 1-31 of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555),
and a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence VSIFYVFI (SEQ ID NO:998) corresponding to amino acids
32-39 of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[0263] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
VSIFYVFI (SEQ ID NO:998) in HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30
(SEQ ID NO:555).
[0264] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P2 (SEQ ID NO:14), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY
GPDVPTISPSKANYRPGENLNLSCHMSNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS
YMCQAHNSATGLNRTTVTMITVS corresponding to amino acids 1-319 of
CEA6_HUMAN (SEQ ID NO:13), which also corresponds to amino acids
1-319 of T10888_PEA.sub.--1_P2 (SEQ ID NO:14), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence DWTRP (SEQ ID NO:999) corresponding to amino acids 320-324
of T10888_PEA.sub.--1_P2 (SEQ ID NO:14), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0265] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P2 (SEQ ID NO:14), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence DWTRP (SEQ
ID NO:999) in T10888_PEA.sub.--1_P2 (SEQ ID NO:14).
[0266] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL
corresponding to amino acids 1-234 of CEA6_HUMAN (SEQ ID NO:13),
which also corresponds to amino acids 1-234 of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) corresponding to amino
acids 235-256 of T10888_PEA.sub.--1_P4 (SEQ ID NO:15), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0267] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) in T10888_PEA.sub.--1_P4
(SEQ ID NO:15).
[0268] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL
corresponding to amino acids 1-234 of Q13774, which also
corresponds to amino acids 1-234 of T10888_PEA.sub.--1_P4 (SEQ ID
NO:15), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID
NO:1000) corresponding to amino acids 235-256 of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0269] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) in T10888_PEA.sub.--1_P4
(SEQ ID NO:15).
[0270] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY
GPDVPTISPSKANYRPGENLNLSCHMSNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS
YMCQAHNSATGLNRTTVTMITVSG corresponding to amino acids 1-320 of
CEA6_HUMAN (SEQ ID NO:13), which also corresponds to amino acids
1-320 of T10888_PEA.sub.--1_P5 (SEQ ID NO:16), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence
KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF
VVFCFLISHV (SEQ ID NO:1001) corresponding to amino acids 321-390 of
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0271] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00078
KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF (SEQ
ID NO: 1001) VVFCFLISHV in T10888_PEA.sub.--1_P5. (SEQ ID NO:
16)
[0272] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), comprising a first amino acid
sequence being at least 90% homologous to TABLE-US-00079
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLA
HNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQ
NDTGFYTLQVIKSDLVNEEATGQFHVY
[0273] corresponding to amino acids 1-141 of CEA6_HUMAN (SEQ ID
NO:13), which also corresponds to amino acids 1-141 of
T10888_PEA.sub.131_P6 (SEQ ID NO:17), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TABLE-US-00080 (SEQ ID NO: 1002)
REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI
[0274] corresponding to amino acids 142-183 of
T10888_PEA.sub.--1_P6 (SEQ ID NO: 17), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0275] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO:1002) in
T10888_PEA.sub.--1_P6 (SEQ ID NO:17.
[0276] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P6 (SEQ ID NO:51), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKG corresponding to amino
acids 1-276 of VTNC_HUMAN, which also corresponds to amino acids
1-276 of T39971_P6 (SEQ ID NO:51), and a second amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence TQGVVGD (SEQ ID
NO:1003) corresponding to amino acids 277-283 of T39971_P6 (SEQ ID
NO:51), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0277] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T39971_P6 (SEQ ID NO:51), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence TQGVVGD (SEQ ID NO:1003) in
T39971_P6 (SEQ ID NO:51).
[0278] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P9 (SEQ ID NO:52), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDMLALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRT corresponding to amino acids
1-325 of VTNC_HUMAN, which also corresponds to amino acids 1-325 of
T39971_P9 (SEQ ID NO:52), and a second amino acid sequence being at
least 90% homologous to
SGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGA
NNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGC PAPGHL
corresponding to amino acids 357-478 of VTNC_HUMAN, which also
corresponds to amino acids 326-447 of T39971_P9 (SEQ ID NO:52),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0279] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T39971_P9 (SEQ ID NO:52) comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise TS, having a structure as follows: a sequence
starting from any of amino acid numbers 325-x to 325; and ending at
any of amino acid numbers 326+((n-2)-x), in which x varies from 0
to n-2.
[0280] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P11 (SEQ ID NO:53), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids
1-326 of VTNC_HUMAN, which also corresponds to amino acids 1-326 of
T39971_P11 (SEQ ID NO:53), and a second amino acid sequence being
at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
corresponding to amino acids 442-478 of VTNC_HUMAN, which also
corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO:53),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0281] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T39971_P11 (SEQ ID NO:53), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise SD, having a structure as follows: a sequence
starting from any of amino acid numbers 326-x to 326; and ending at
any of amino acid numbers 327+((n-2)-x), in which x varies from 0
to n-2.
[0282] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P11 (SEQ ID NO:53), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDMLALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids
1-326 of Q9BSH7, which also corresponds to amino acids 1-326 of
T39971_P11 (SEQ ID NO:53), and a second amino acid sequence being
at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
corresponding to amino acids 442-478 of Q9BSH7, which also
corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO:53),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0283] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T39971_P11 (SEQ ID NO:53), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise SD, having a structure as follows: a sequence
starting from any of amino acid numbers 326-x to 326; and ending at
any of amino acid numbers 327+((n-2)-x), in which x varies from 0
to n-2.
[0284] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P12 (SEQ ID NO:54), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to
amino acids 1-223 of VTNC_HUMAN, which also corresponds to amino
acids 1-223 of T39971_P12 (SEQ ID NO:54), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VPGAVGQGRKHLGRV (SEQ ID NO:1004) corresponding to amino acids
224-238 of T39971_P12 (SEQ ID NO:54, wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0285] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T39971_P12 (SEQ ID NO:54), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID
NO:1004) in T39971_P12 (SEQ ID NO:54)
[0286] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T39971_P12 (SEQ ID NO:54), comprising a first amino acid sequence
being at least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to
amino acids 1-223 of Q9BSH7, which also corresponds to amino acids
1-223 of T39971_P12 (SEQ ID NO:54), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VPGAVGQGRKHLGRV (SEQ ID NO:1004) corresponding to amino acids
224-238 of T39971_P12 (SEQ ID NO:54), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0287] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T39971_P12 (SEQ ID NO:54), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID
NO:1004) in T39971_P12 (SEQ ID NO:54).
[0288] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFL
VERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGK
LRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQ
GTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQ
ASSGGNRGRMLADSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYI
DKEIEALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKE
AAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWN
corresponding to amino acids 1-761 of SUL1_HUMAN, which also
corresponds to amino acids 1-761 of Z21368_PEA.sub.--1_P2 (SEQ ID
NO:97), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence PHKYSAHGRTRHFESATRTTNGAQKLSRI (SEQ
ID NO:1005) corresponding to amino acids 762-790 of
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0289] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
PHKYSAHGRTRHFESATRTTNGAQKLSRI (SEQ ID NO:1005) in
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97).
[0290] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVEL
corresponding to amino acids 1-57 of Q7Z2W2 (SEQ ID NO:840), which
also corresponds to amino acids 1-57 of Z21368_PEA.sub.--1_P5 (SEQ
ID NO:98), second bridging amino acid sequence comprising A, and a
third amino acid sequence being at least 90% homologous to
FFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITN
ESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNM
DKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYT
ADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDT
PPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHL
PKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLY
ARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFE
GEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPT
TVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKR
RKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEMQEVDSKLQLFKENNRRRKKER
KEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNE
THNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCN
PRPKNLDVGNKDGGSYDLHRGQLWDGWEG corresponding to amino acids 139-871
of Q7Z2W2 (SEQ ID NO:840), which also corresponds to amino acids
59-791 of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein said first,
second and third amino acid sequences are contiguous and in a
sequential order.
[0291] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for an edge
portion of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least three amino acids comprise LAF having a structure as follows
(numbering according to Z21368_PEA.sub.--1_P5 (SEQ ID NO:98)): a
sequence starting from any of amino acid numbers 57-x to 57; and
ending at any of amino acid numbers 59+((n-2)-x), in which x varies
from 0 to n-2.
[0292] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELAFF
GKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNES
INYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDK
HWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTAD
HGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPP
DVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLP
KYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYA
RGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGE
IYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTV
RVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRK
PEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRKKERKE
KRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETH
NFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLME (SEQ ID NO:1006)
corresponding to amino acids 1-751 of Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98), and a second amino acid sequence being at least 90%
homologous to LRSCQGYKQCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG
corresponding to amino acids 1-40 of AAH12997 (SEQ ID NO:841),
which also corresponds to amino acids 752-791 of
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0293] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00081
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELAFF (SEQ
ID NO: 1006)
GKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNES
INYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDK
HWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTAD
HGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPP
DVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLP
KYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYA
RGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGE
IYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTV
RVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRK
PEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRKKERKE
KRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETH
NFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLME of
Z21368_PEA.sub.--1_P5. (SEQ ID NO: 98)
[0294] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVEL
corresponding to amino acids 1-57 of SUL1_HUMAN, which also
corresponds to amino acids 1-57 of Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98), and a second amino acid sequence being at least 90%
homologous to
AFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLIT
NESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQFSKLYPNASQHITPSYNYAPN
MDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYII
YTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGL
DTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSN
HLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRN
LYARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVE
FEGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGP
PTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHL
KRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEMQEVDSKLQLFKENNRRRK
KERKEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRT
VNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYK
QCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG corresponding to amino acids
138-871 of SUL1_HUMAN, which also corresponds to amino acids 58-791
of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0295] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise LA, having a structure as follows: a
sequence starting from any of amino acid numbers 57-x to 57; and
ending at any of amino acid numbers 58+((n-2)-x), in which x varies
from 0 to n-2.
[0296] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P15 (SEQ ID NO:99), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERG
corresponding to amino acids 1-416 of SUL1_HUMAN, which also
corresponds to amino acids 1-416 of Z21368_PEA.sub.--1_P15 (SEQ ID
NO:99).
[0297] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P16 (SEQ ID NO:100), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNR corresponding to
amino acids 1-397 of SUL1_HUMAN, which also corresponds to amino
acids 1-397 of Z21368_PEA.sub.--1_P16 (SEQ ID NO:100), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence CVIVPPLSQPQIH (SEQ ID NO:1007) corresponding to amino
acids 398-410 of Z21368_PEA.sub.--1_P16 (SEQ ID NO:100), wherein
said first and second amino acid sequences are contiguous and in a
sequential order.
[0298] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P16 (SEQ ID NO:100), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
CVIVPPLSQPQIH (SEQ ID NO:1007) in Z21368_PEA.sub.--1-P16 (SEQ ID
NO:100).
[0299] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAK corresponding to amino acids 1-188 of SUL1_HUMAN,
which also corresponds to amino acids 1-188 of
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ARYDGDQPRCAPRPRGLSPTVF (SEQ ID NO:1008) corresponding to amino
acids 189-210 of Z21368_PEA.sub.--1_P22 (SEQ ID NO: 101), wherein
said first and second amino acid sequences are contiguous and in a
sequential order.
[0300] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably, at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ARYDGDQPRCAPRPRGLSPTVF (SEQ ID NO:1008) in Z21368_PEA.sub.--1_P22
(SEQ ID NO:101).
[0301] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRT corresponding to amino acids 1-137 of Q7Z2W2
(SEQ ID NO:840), which also corresponds to amino acids 1-137 of
Z21368_PEA.sub.--1_P23 (SEQ ID NO: 102), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence GLLHRLNH
(SEQ ID NO:1009) corresponding to amino acids 138-145 of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0302] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GLLHRLNH
(SEQ ID NO:1009) in Z21368_PEA.sub.--1_P23 (SEQ ID NO:102).
[0303] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P23 (SEB ID NO:102), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRT corresponding to amino acids 1-137 of
SUL1_HUMAN, which also corresponds to amino acids 1-137 of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence GLLHRLNH
(SEQ ID NO:1009) corresponding to amino acids 138-145 of Z21368
PEA.sub.--1_P23 (SEQ ID NO:102), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0304] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GLLHRLNH
(SEQ ID NO:1009) in Z21368_PEA.sub.--1_P23 (SEQ ID NO:102).
[0305] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P5 (SEQ ID NO:143), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 12-55 of GILT_HUMAN (SEQ ID NO:142), which also corresponds
to amino acids 1-44 of T59832_P5 (SEQ ID NO:143), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP
SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSMGQGPPACIL
AATLDAFIPARAGLACLWDLLGRCPRG (SEQ ID NO:1010) corresponding to amino
acids 45-189 of T59832_P5 (SEQ ID NO:143), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0306] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P5 (SEQ ID NO: 143), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence TABLE-US-00082
VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP (SEQ ID
NO: 1010)
SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSAAGQGPPACIL
AATLDAFIPARAGLACLWDLLGRCPRG in T59832_P5. (SEQ ID NO: 143)
[0307] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P7 (SEQ ID NO:144), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
12-223 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding
to amino acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0308] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P7 (SEQ ID NO:144), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR
(SEQ ID NO:1011) in T59832_P7 (SEQ ID NO:144).
[0309] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P7 (SEQ ID NO:144), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
1-212 of BAC98466 (SEQ ID NO:848), which also corresponds to amino
acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding to amino
acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0310] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P7 (SEQ ID NO:144), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR
(SEQ ID NO:1011) in T59832_P7 (SEQ ID NO:144).
[0311] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P7 (SEQ ID NO:144), comprising a first amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P7 (SEQ ID NO:144), and a second amino
acid sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC
MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYV
PWVTVNGVRIFLALSLTLIVPWSQGWTRQRDQR corresponding to amino acids
1-148 of BAC85622 (SEQ ID NO:849), which also corresponds to amino
acids 91-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0312] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
T59832_P7 (SEQ ID NO:144), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence TABLE-US-00083
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832_P7. (SEQ ID NO:
144)
[0313] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P7 (SEQ ID NO:144), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
1-212 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding to amino
acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0314] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P7 (SEQ ID NO:144), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR
(SEQ ID NO:1011) in T59832_P7 (SEQ ID NO:144).
[0315] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P9 (SEQ ID NO:145), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 12-214 of
GILT_HUMAN (SEQ ID NO:142), which also corresponds to amino acids
1-203 of T59832_P9 (SEQ ID NO:145), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013)
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0316] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P9 (SEQ ID NO:145), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[0317] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P9 (SEQ ID NO:145), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of
BAC98466 (SEQ ID NO:848), which also corresponds to amino acids
1-203 of T59832_P9 (SEQ ID NO:145), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013)
corresponding to amino acids 204-244 of T59832 P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0318] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832 P9 (SEQ ID NO: 145), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[0319] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P9 (SEQ ID NO:145), comprising a first amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P9 (SEQ ID NO:145), second amino acid
sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC
MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHE
corresponding to amino acids 1-113 of BAC85622 (SEQ ID NO:849),
which also corresponds to amino acids 91-203 of T59832_P9 (SEQ ID
NO:145), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013)
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first, second and third amino acid sequences are
contiguous and in a sequential order.
[0320] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
T59832_P9 (SEQ ID NO:145), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence TABLE-US-00084
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832 P9. (SEQ ID NO:
145)
[0321] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P9 (SEQ ID NO:145), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[0322] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P9 (SEQ ID NO:145), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of
Q8WU77 (SEQ ID NO:850), which also corresponds to amino acids 1-203
of T59832_P9 (SEQ ID NO:145), and a second amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence TABLE-US-00085 (SEQ
ID NO: 1013) NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0323] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P9 (SEQ ID NO:145), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[0324] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P12 (SEQ ID NO:146), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVE corresponding to amino acids 12-141 of GILT_HUMAN
(SEQ ID NO:142), which also corresponds to amino acids 1-130 of
T59832_P12 (SEQ ID NO:146), and a second amino acid sequence being
at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
173-261 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 131-219 of T59832_P12 (SEQ ID NO:146), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0325] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_P12 (SEQ ID NO:146), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EC, having a structure as follows: a
sequence starting from any of amino acid numbers 130-x to 130; and
ending at any of amino acid numbers 131+((n-2)-x), in which x
varies from 0 to n-2.
[0326] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P12 (SEQ ID NO:146), comprising a first amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P12 (SEQ ID NO:146), second amino acid
sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVE corresponding to amino
acids 1-40 of BAC85622 (SEQ ID NO:849), which also corresponds to
amino acids 91-130 of T59832_P12 (SEQ ID NO:146), third amino acid
sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding
to amino acids 72-122 of BAC85622 (SEQ ID NO:849), which also
corresponds to amino acids 131-181 of T59832_P 12 (SEQ ID NO:146),
and a fourth amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID
NO:1016) corresponding to amino acids 182-219 of T59832_P12 (SEQ ID
NO:146), wherein said first, second, third and fourth amino acid
sequences are contiguous and in a sequential order.
[0327] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a head of
T59832_P12 (SEQ ID NO:146), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence TABLE-US-00086
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832_P12. (SEQ ID
NO: 146)
[0328] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_P12 (SEQ ID NO:146), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EC, having a structure as follows: a
sequence starting from any of amino acid numbers 130-x to 130; and
ending at any of amino acid numbers 131+((n-2)-x), in which x
varies from 0 to n-2.
[0329] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T59832_P 12 (SEQ ID NO:146), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence
KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID NO:1016) in
T59832_P12 (SEQ ID NO:146).
[0330] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P12 (SEQ ID NO:146), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVE corresponding to amino acids 1-130 of Q8WU77 (SEQ ID
NO:850), which also corresponds to amino acids 1-130 of T59832_P12
(SEQ ID NO:146), and a second amino acid sequence being at least
90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 131-219 of T59832_P12 (SEQ ID NO:146), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0331] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_P12 (SEQ ID NO:146), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EC, having a structure as follows: a
sequence starting from any of amino acid numbers 130-x to 130; and
ending at any of amino acid numbers 131+((n-2)-x), in which x
varies from 0 to n-2.
[0332] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P18 (SEQ ID NO:147), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 12-55 of GILT_HUMAN (SEQ ID NO:142), which also corresponds
to amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second
amino acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
173-261 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0333] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_PI18 (SEQ ID NO:147), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise KC, having a structure as follows: a
sequence starting from any of amino acid numbers 44-x to 44; and
ending at any of amino acid numbers 45+((n-2)-x), in which x varies
from 0 to n-2.
[0334] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P18 (SEQ ID NO:147), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 1-44 of Q8WU77 (SEQ ID NO:850), which also corresponds to
amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second amino
acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0335] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_P18 (SEQ ID NO:147), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise KC, having a structure as follows: a
sequence starting from any of amino acid numbers 44-x to 44; and
ending at any of amino acid numbers 45+((n-2)-x), in which x varies
from 0 to n-2.
[0336] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T59832_P18 (SEQ ID NO:147), comprising a first amino acid sequence
being at least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 1-44 of Q8NE14 (SEQ ID NO:851), which also corresponds to
amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second amino
acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8NE14 (SEQ ID NO:851), which also corresponds to amino
acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0337] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of T59832_P18 (SEQ ID NO:147), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise KC, having a structure as follows: a
sequence starting from any of amino acid numbers 44-x to 44; and
ending at any of amino acid numbers 45+((n-2)-x), in which x varies
from 0 to n-2.
[0338] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMGRP5E-P4 (SEQ ID NO:1566), comprising a first amino acid
sequence being at least 90% homologous to
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG
ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED
SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN, which
also corresponds to amino acids 1-127 of HUMGRP5E_P4 (SEQ ID
NO:156), and a second amino acid sequence being at least 90%
homologous to GSQREGRNPQLNQQ corresponding to amino acids 135-148
of GRP_HUMAN, which also corresponds to amino acids 128-141 of
HUMGRP5E_P4 (SEQ ID NO:156), wherein said first and second amino
acid sequences are contiguous and in a sequential order.
[0339] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of HUMGRP5E_P4 (SEQ ID NO:156), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise KG, having a structure as follows: a
sequence starting from any of amino acid numbers 127-x to 127; and
ending at any of amino acid numbers 128+((n-2)-x), in which x
varies from 0 to n-2.
[0340] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HUMGRP5E_P5 (SEQ ID NO:157), comprising a first amino acid sequence
being at least 90% homologous to
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG
ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED
SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN, which
also corresponds to amino acids 1-127 of HUMGRP5E_P5 (SEQ ID
NO:157), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DSLLQVLNVKEGTPS (SEQ ID NO:1017)
corresponding to amino acids 128-142 of HUMGRP5E_P5 (SEQ ID
NO:157), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0341] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
HUMGRP5E_P5 (SEQ ID NO:157), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence DSLLQVLNVKEGTPS (SEQ ID
NO:1017) in HUMGRP5E_P5 (SEQ ID NO:157).
[0342] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEMLLPQNDTRLDPEAYGAPCARGSQ
PWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDHLLLLQGEQLRRTT
RSWHPKYHQGSGPILPRRTDEHDLMLLKLARP corresponding to amino acids 1-146
of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino
acids 1-146 of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), and a
second amino acid sequence being at least 90% homologous to
YNKGLTCSSITILSPKECEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGIL
SWGVYPCGSAQHPAVYTQICKYMSWINKVIRSN corresponding to amino acids
184-276 of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to
amino acids 147-239 of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0343] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), comprising
a polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise PY, having a structure as follows: a
sequence starting from any of amino acid numbers 146-x to 146; and
ending at any of amino acid numbers 147+((n-2)-x), in which x
varies from 0 to n-2.
[0344] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P6 (SEQ ID NO:179), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSAASGARALAKLLPLLMAQLW corresponding to amino acids 1-29 of
KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino acids
1-29 of AA155578_PEA.sub.--1 P6 (SEQ ID NO:179), and a second amino
acid sequence being at least 90% homologous to
VKYNKGLTCSSITILSPKECEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQ
GILSWGVYPCGSAQHPAVYTQICKYMSWINKVIRSN corresponding to amino acids
182-276 of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to
amino acids 30-124 of AA155578_PEA.sub.--1_P6 (SEQ ID NO:179),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0345] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for an
edge portion of AA155578_PEA.sub.--1_P6 (SEQ ID NO: 179),
comprising a polypeptide having a length "n", wherein n is at least
about 10 amino acids in length, optionally at least about 20 amino
acids in length, preferably at least about 30 amino acids in
length, more preferably at least about 40 amino acids in length and
most preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise WV, having a structure as follows: a
sequence starting from any of amino acid numbers 29-x to 29; and
ending at any of amino acid numbers 30+((n-2)-x), in which x varies
from 0 to n-2.
[0346] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSMSGARALAKLLPLLMAQLW corresponding to amino acids 1-29 of
KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino acids
1-29 of AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence GHCGLE (SEQ ID NO:1018) corresponding to amino acids 30-35
of AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0347] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
M155578-PEA.sub.--1_P8 (SEQ ID NO:180), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GHCGLE
(SEQ ID NO:1018) in AA155578_PEA.sub.--1_P8 (SEQ ID NO:180).
[0348] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P9 (SEQ ID NO:181), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSMSGARALAKLLPLLMAQLWMEMLLPQNDTRLDPEAYGAPCARGSQ
PWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNK corresponding to amino acids 1-90
of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino
acids 1-90 of AA155578_PEA.sub.--1.sub.--P9 (SEQ ID NO:181).
[0349] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
HSENA78_P2 (SEQ ID NO:919), comprising a first amino acid sequence
being at least 90% homologous to
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCVCLQTTQGVHP
KMISNLQVFAIGPQCSKVEW corresponding to amino acids 1-81 of
SZ05_HUMAN (SEQ ID NO:190), which also corresponds to amino acids
1-81 of HSENA78_P2 (SEQ ID NO:191) According to preferred
embodiments of the present invention, there is provided an isolated
chimeric polypeptide encoding for T94936_PEA.sub.--1_P2 (SEQ ID
NO:206), comprising a first amino acid sequence being at least 90%
homologous to
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKS
KKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQY
VPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPL corresponding to amino acids
1-150 of Q8TD06 (SEQ ID NO:858), which also corresponds to amino
acids 1-150 of T94936_PEA.sub.--1_P2 (SEQ ID NO:206).
[0350] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
T94936_PEA.sub.--1_P3 (SEQ ID NO:207), comprising a first amino
acid sequence being at least 90% homologous to
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKS
KKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQY VPRIMFV
corresponding to amino acids 1-122 of Q8TD06 (SEQ ID NO:858), which
also corresponds to amino acids 1-122 of T94936_PEA.sub.--1_P3 (SEQ
ID NO:207), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence GMYVISFHQIYKISRNQHSCFYF (SEQ ID
NO:1019) corresponding to amino acids 123-145 of
T94936_PEA.sub.--1_P3 (SEQ ID NO:207), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0351] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
T94936_PEA.sub.--1_P3 (SEQ ID NO:207), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GMYVISFHQIYKISRNQHSCFYF (SEQ ID NO:1019) in T94936_PEA.sub.--1_P3
(SEQ ID NO:207).
[0352] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
1-95 of SZ14_HUMAN (SEQ ID NO:230), which also corresponds to amino
acids 1-95 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020)
corresponding to amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ
ID NO:231), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0353] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1-P10 (SEQ ID NO:231).
[0354] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
13-107 of Q9NS21 (SEQ ID NO:862), which also corresponds to amino
acids 1-95 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020)
corresponding to amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ
ID NO:231), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0355] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231).
[0356] According to preferred embodiments of the present invention,
there is provided an isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
13-107 of AAQ89265, which also corresponds to amino acids 1-95 of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) corresponding to
amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0357] According to preferred embodiments of the present invention,
there is provided an isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1.sub.--P10(SEQ ID NO:231).
[0358] According to preferred embodiments of the present invention,
there is provided an isolated oligonucleotide, comprising an
amplicon selected from the group consisting of SEQ ID NOs: 891 or
894.
[0359] According to preferred embodiments of the present invention,
there is provided a primer pair, comprising a pair of isolated
oligonucleotides capable of amplifying the above. Optionally, the
pair of isolated oligonucleotides is selected from the group
consisting of: SEQ NOs 889 and 890; or 892 and 893.
[0360] According to preferred embodiments of the present invention,
there is provided an antibody capable of specifically binding to an
epitope of an amino acid sequence as described herein. Optionally,
the epitope may comprise a tail, head, or edge portion as described
herein.
[0361] According to preferred embodiments of the present invention,
the antibody is capable of differentiating between a splice variant
having said epitope and a corresponding known protein.
[0362] According to preferred embodiments of the present invention,
there is provided an kit for detecting breast cancer, comprising a
kit detecting overexpression of a splice variant as described
herein. Optionally, the kit comprises a NAT-based technology.
Preferably, the kit further comprises at least one primer pair
capable of selectively hybridizing to a nucleic acid sequence as
described herein. Optionally, the kit further comprises at least
one oligonucleotide capable of selectively hybridizing to a nucleic
acid sequence as described herein.
[0363] Optionally, the kit comprises an antibody as described
herein. Preferably, the kit further comprises at least one reagent
for performing an ELISA or a Western blot.
[0364] According to preferred embodiments of the present invention,
there is provided a method for detecting breast cancer, comprising
detecting overexpression of a splice variant as described
herein.
[0365] Optionally detecting overexpression is performed with a
NAT-based technology. Preferably, detecting overexpression is
performed with an immunoassay. More preferably, the immunoassay
comprises an antibody as described herein.
[0366] According to preferred embodiments of the present invention,
there is provided a biomarker capable of detecting breast cancer,
comprising any of the above nucleic acid sequences or a fragment
thereof, or any of the above amino acid sequences or a fragment
thereof.
[0367] According to preferred embodiments of the present invention,
preferably any of the above nucleic acid and/or amino acid
sequences further comprises any sequence having at least about 70%,
preferably at least about 80%, more preferably at least about 90%,
most preferably at least about 95% homology thereto.
[0368] Unless otherwise noted, all experimental data relates to
variants of the present invention, named according to the segment
being tested (as expression was tested through RT-PCR as
described).
[0369] All nucleic acid sequences and/or amino acid sequences shown
herein as embodiments of the present invention relate to their
isolated form, as isolated polynucleotides (including for all
transcripts), oligonucleotides (including for all segments,
amplicons and primers), peptides (including for all tails, bridges,
insertions or heads, optionally including other antibody epitopes
as described herein) and/or polypeptides (including for all
proteins). It should be noted that oligonucleotide and
polynucleotide, or peptide and polypeptide, may optionally be used
interchangeably.
[0370] Unless defined otherwise, all technical and scientific terms
used herein have the meaning commonly understood by a person
skilled in the art to which this invention belongs. The following
references provide one of skill with a general definition of many
of the terms used in this invention: Singleton et al., Dictionary
of Microbiology and Molecular Biology (2nd ed. 1994); The Cambridge
Dictionary of Science and Technology (Walker ed., 1988); The
Glossary of Genetics, 5th Ed., R. Rieger et al. (eds.), Springer
Verlag (1991); and Hale & Marham, The Harper Collins Dictionary
of Biology (1991). All of these are hereby incorporated by
reference as if fully set forth herein. As used herein, the
following terms have the meanings ascribed to them unless specified
otherwise.
BRIEF DESCRIPTION OF DRAWINGS
[0371] FIG. 1 is schematic summary of cancer biomarkers selection
engine and the wet validation stages.
[0372] FIG. 2. Schematic illustration, depicting grouping of
transcripts of a given cluster based on presence or absence of
unique sequence regions.
[0373] FIG. 3 is schematic summary of quantitative real-time PCR
analysis.
[0374] FIG. 4 is schematic presentation of the oligonucleotide
based microarray fabrication.
[0375] FIG. 5 is schematic summary of the oligonucleotide based
microarray experimental flow.
[0376] FIG. 6 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster T10888, demonstrating
overexpression in colorectal cancer, a mixture of malignant tumors
from different tissues, pancreas carcinoma and gastric
carcinoma.
[0377] FIG. 7 is a histogram showing expression of the CEA6_HUMAN
(SEQ ID NO:13) Carcinoembryonic antigen-related cell adhesion
molecule 6 (T10888) transcripts, which are detectable by amplicon
as depicted in sequence name T10888 junc11-17, in normal and
cancerous breast tissues.
[0378] FIG. 8 is a histogram showing the expression of CEA6_HUMAN
(SEQ ID NO:13) Carcinoembryonic antigen-related cell adhesion
molecule 6 T10888 transcripts which are detectable by amplicon as
depicted in sequence name T10888junc11-17 (SEQ ID NO:832) in
different normal tissues.
[0379] FIG. 9 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster T39971, demonstrating
overexpression in liver cancer, lung malignant tumors and pancreas
carcinoma.
[0380] FIG. 10 is a histogram showing the expression of VTNC_HUMAN
vitronectin (serum spreading factor, somatomedin B, complement
S-protein) T39971 transcripts, which are detectable by amplicon as
depicted in sequence name T39971 junc23-33 (SEQ ID NO:836) in
normal and cancerous breast tissues.
[0381] FIG. 11 is a histogram showing the expression of VTNC_HUMAN
vitronectin (serum spreading factor, somatomedin B, complement
S-protein), antisense to SARM1 (T23434), T39971 transcripts, which
are detectable by amplicon as depicted in sequence name
T39971junc23-33, in different normal tissues.
[0382] FIG. 12 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster Z21368, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues and pancreas carcinoma.
[0383] FIG. 13 is a histogram showing the expression of
SULL_HUMAN--Extracellular sulfatase Sulf-1Z21368 transcripts, which
are detectable by amplicon as depicted in sequence name Z21368seg39
(SEQ ID NO:844), in normal and cancerous breast tissues.
[0384] FIG. 14 is a histogram showing the expression of
SUL1_HUMAN--Extracellular sulfatase Sulf-1Z21368 transcripts, which
are detectable by amplicon as depicted in sequence name Z21368seg39
(SEQ ID NO:844), in different normal tissues.
[0385] FIG. 15 is a histogram showing the expression of SUL
1_HUMAN--Extracellular sulfatase Sulf-1Z21368 transcripts which are
detectable by amplicon as depicted in sequence name Z21368junc17-21
(SEQ ID NO:847) in normal and cancerous breast tissues.
[0386] FIG. 16 is a histogram showing the expression of SUL
1_HUMAN--Extracellular sulfatase Sulf-1Z21368 transcripts, which
are detectable by amplicon as depicted in sequence name
Z21368junc17-21, in different normal tissues.
[0387] FIG. 17 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster T59832, demonstrating
overexpression in brain malignant tumors, breast malignant tumors,
ovarian carcinoma and pancreas carcinoma.
[0388] FIG. 18 is a histogram showing low over expression observed
for cluster T59832, amplicon name: T59832 junc6-25-26 (SEQ ID
NO:854), in one experiment carried out with breast cancer samples
panel.
[0389] FIG. 19 is a histogram showing the expression of
GRP_HUMAN--gastrin-releasing peptide (HUMGRP5E) transcripts, which
are detectable by amplicon, as depicted insequence name
HUMGRP5Ejunc3-7 (SEQ ID NO:857) in normal and cancerous breast
tissues.
[0390] FIG. 20 is a histogram showing the expression of
GRP_HUMAN--gastrin-releasing peptide (HUMGRP5E) transcripts, which
are detectable by amplicon, as depicted in sequence name
HUMGRP5Ejunc3-7 (SEQ ID NO:857), in different normal tissues.
[0391] FIG. 21 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster AA155578, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues and pancreas carcinoma.
[0392] FIG. 22 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSENA78, demonstrating
overexpression in epithelial malignant tumors and lung malignant
tumors.
[0393] FIG. 23 is a histogram showing the expression of Homo
sapiens breast cancer membrane protein 11 (BCMP11) T94936
transcripts which are detectable by amplicon as depicted in
sequence name T94936 seg14 (SEQ ID NO:861) in normal and cancerous
Breast tissues.
[0394] FIG. 24 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster Z41644, demonstrating
overexpression in lung malignant tumors, breast malignant tumors
and pancreas carcinoma.
[0395] FIG. 25 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster M85491, demonstrating
overexpression in epithelial malignant tumors and a mixture of
malignant tumors from different tissues.
[0396] FIG. 26 is a histogram showing the expression of Ephrin
type-B receptor 2 precursor (EC 2.7.1.112) (Tyrosine-protein kinase
receptor EPH-3) M85491 transcripts which are detectable by amplicon
as depicted in sequence name M85491seg24 (SEQ ID NO:866) in normal
and cancerous breast tissues.
[0397] FIG. 27 is a histogram showing the expression of Ephrin
type-B receptor 2 precursor M85491 transcripts, which are
detectable by amplicon as depicted in sequence name M85491 seg24,
in different normal tissues.
[0398] FIG. 28 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSSTROL3, demonstrating
overexpression in transitional cell carcinoma, epithelial malignant
tumors, a mixture of malignant tumors from different tissues and
pancreas carcinoma.
[0399] FIG. 29A is a histogram showing the expression of Expression
of Stromelysin-3 precursor (SEQ ID NO:270) (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) SL-3 HSSTROL3 transcripts
which are detectable by amplicon as depicted in sequence name
HSSTROL3 seg24 (SEQ ID NO:869) in normal and cancerous breast
tissues.
[0400] FIG. 29B is a histogram showing the expression of
Stromelysin-3 precursor (SEQ ID NO:270) (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) (SL-3) HSSTROL3 transcripts,
which are detectable by amplicon as depicted in sequence name
HSSTROL3 seg24 (SEQ ID NO:869), in different normal tissues.
[0401] FIGS. 30A-30C shows histograms showing over expression of
various Stromelysin-3 precursor (SEQ ID NO:270) transcripts in
cancerous breast samples relative to the normal samples.
[0402] FIG. 31 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster R75793, demonstrating
overexpression in epithelial malignant tumors and a mixture of
malignant tumors from different tissues.
[0403] FIG. 32 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HUMCA1XIA, demonstrating
overexpression in bone malignant tumors, epithelial malignant
tumors, a mixture of malignant tumors from different tissues and
lung malignant tumors.
[0404] FIG. 33 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster R20779, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues and lung malignant
tumors.
[0405] FIG. 34 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSS100PCB, demonstrating
overexpression in a mixture of malignant tumors from different
tissues.
[0406] FIG. 35 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSCOC4, demonstrating
overexpression in brain malignant tumors, a mixture of malignant
tumors from different-tissues, breast malignant tumors, pancreas
carcinoma and prostate cancer.
[0407] FIG. 36 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HUMTREFAC, demonstrating
overexpression in a mixture of malignant tumors from different
tissues, breast malignant tumors, pancreas carcinoma and prostate
cancer.
[0408] FIG. 37 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HUMOSTRO, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues, lung malignant tumors,
breast malignant tumors, ovarian carcinoma and skin
malignancies.
[0409] FIG. 38 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster R11723, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues and kidney malignant
tumors.
[0410] FIG. 39 is a histogram showing the expression of R11723
transcripts which are detectable by amplicon as depicted in
sequence name R11723 seg13 (SEQ ID NO:891) in normal and cancerous
breast tissues.
[0411] FIG. 40 is a histogram showing the expression of R11723
transcripts, which are detectable by amplicon as depicted in
sequence name R11723seg13 (SEQ ID NO:891), in different normal
tissues.
[0412] FIGS. 41A and B are histograms showing the expression of
R11723 transcripts, which are detectable by amplicon as depicted in
sequence name R11723 junc1-18 (SEQ ID NO:894) in normal and
cancerous breast tissues (FIG. 41A) or on a panel of normal tissues
(FIG. 41B).
[0413] FIG. 42 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster T46984, demonstrating
overexpression in epithelial malignant tumors, a mixture of
malignant tumors from different tissues, breast malignant tumors,
ovarian carcinoma and pancreas carcinoma.
[0414] FIG. 43 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSMUC 1A, demonstrating
overexpression in a mixture of malignant tumors from different
tissues, breast malignant tumors, pancreas carcinoma and prostate
cancer.
[0415] FIGS. 44-47 are histograms showing the combined expression
of 8 sequences (T10888seg11-17, HUMGR5Ejunc3-7, HSSTROL3seg24,
T94936 Seg 14, Z21368 seg39, Z21368junc17-21 T59832jun6-25-26 and
M85491seg24 (SEQ ID NO:866)) in normal and cancerous breast
tissues.
[0416] FIG. 48 is a histogram showing Cancer and cell-line vs.
normal tissue expression for Cluster HSU33147, demonstrating
overexpression in a mixture of malignant tumors from different
tissues.
DESCRIPTION OF PREFERRED EMBODIMENTS
[0417] The present invention is of novel markers for breast cancer
that are both sensitive and accurate. Furthermore, at least certain
of these markers are able to distinguish between different stages
of breast cancer, such as 1. Ductal carcinoma (in-situ, invasive)
2. Lobular carcinoma (is-situ, invasive) 3. inflammatory breast
cancer 4. Mucinous carcinoma 5. Tubular carcinoma 6. Paget's
disease of nipple, alone or in combination; or one of the
indicative conditions described above.
[0418] The markers of the present invention, alone or in
combination, can be used for prognosis, prediction, screening,
early diagnosis, staging, therapy selection and treatment
monitoring of breast cancer. For example, optionally and
preferably, these markers may be used for staging breast cancer
and/or monitoring the progression of the disease. Furthermore, the
markers of the present invention, alone or in combination, can be
used for detection of the source of metastasis found in anatomical
places other then breast. Also, one or more of the markers may
optionally be used in combination with one or more other breast
cancer markers (other than those described herein).
[0419] Biomolecular sequences (amino acid and/or nucleic acid
sequences) uncovered using the methodology of the present invention
and described herein can be efficiently utilized as tissue or
pathological markers and/or as drugs or drug targets for treating
or preventing a disease.
[0420] These markers are specifically released to the bloodstream
under conditions of breast cancer (or one of the above indicative
conditions), and/or are otherwise expressed at a much higher level
and/or specifically expressed in breast cancer tissue or cells,
and/or tissue or cells under one of the above indicative
conditions. The measurement of these markers, alone or in
combination, in patient samples provides information that the
diagnostician can correlate with a probable diagnosis of breast
cancer and/or a condition that it is indicative of a higher risk
for breast cancer.
[0421] The present invention therefore also relates to diagnostic
assays for breast cancer and/or an indicative condition, and
methods of use of such markers for detection of breast cancer
and/or an indicative condition, optionally and preferably in a
sample taken from a subject (patient), which is more preferably
some type of blood sample.
[0422] According to a preferred embodiment of the present
invention, use of the marker optionally and preferably permits a
non-cancerous breast disease state to be distinguished from breast
cancer and/or an indicative condition. A non limiting example of a
non-cancerous breast disease state includes breast fibrosis and/or
cysts. According to another preferred embodiment of the present
invention, use of the marker optionally and preferably permits an
indicative condition to be distinguished from breast cancer.
[0423] In another embodiment, the present invention relates to
bridges, tails, heads and/or insertions, and/or analogs, homologs
and derivatives of such peptides. Such bridges, tails, heads and/or
insertions are described in greater detail below with regard to the
Examples.
[0424] As used herein a "tail" refers to a peptide sequence at the
end of an amino acid sequence that is unique to a splice variant
according to the present invention. Therefore, a splice variant
having such a tail may optionally be considered as a chimera, in
that at least a first portion of the splice variant is typically
highly homologous (often 100% identical) to a portion of the
corresponding known protein, while at least a second portion of the
variant comprises the tail.
[0425] As used herein a "head" refers to a peptide sequence at the
beginning of an amino acid sequence that is unique to a splice
variant according to the present invention. Therefore, a splice
variant having such a head may optionally be considered as a
chimera, in that at least a first portion of the splice variant
comprises the head, while at least a second portion is typically
highly homologous (often 100% identical) to a portion of the
corresponding known protein.
[0426] As used herein "an edge portion" refers to a connection
between two portions of a splice variant according to the present
invention that were not joined in the wild type or known protein.
An edge may optionally arise due to a join between the above "known
protein" portion of a variant and the tail, for example, and/or may
occur if an internal portion of the wild type sequence is no longer
present, such that two portions of the sequence are now contiguous
in the splice variant that were not contiguous in the known
protein. A "bridge" may optionally be an edge portion as described
above, but may also include a join between a head and a "known
protein" portion of a variant, or a join between a tail and a
"known protein" portion of a variant, or a join between an
insertion and a "known protein" portion of a variant.
[0427] Optionally and preferably, a bridge between a tail or a head
or a unique insertion, and a "known protein" portion of a variant,
comprises at least about 10 amino acids, more preferably at least
about 20 amino acids, most preferably at least about 30 amino
acids, and even more preferably at least about 40 amino acids, in
which at least one amino acid is from the tail/head/insertion and
at least one amino acid is from the "known protein" portion of a
variant. Also optionally, the bridge may comprise any number of
amino acids from about 10 to about 40 amino acids (for example, 10,
11, 12, 13 . . . 37, 38, 39, 40 amino acids in length, or any
number in between).
[0428] It should be noted that a bridge cannot be extended beyond
the length of the sequence in either direction, and it should be
assumed that every bridge description is to be read in such manner
that the bridge length does not extend beyond the sequence
itself.
[0429] Furthermore, bridges are described with regard to a sliding
window in certain contexts below. For example, certain descriptions
of the bridges feature the following format: a bridge between two
edges (in which a portion of the known protein is not present in
the variant) may optionally be described as follows: a bridge
portion of CONTIG-NAME_P1 (representing the name of the protein),
comprising a polypeptide having a length "n", wherein n is at least
about 10 amino acids in length, optionally at least about 20 amino
acids in length, preferably at least about 30 amino acids in
length, more preferably at least about 40 amino acids in length and
most preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise XX (2 amino acids in the center of
the bridge, one from each end of the edge), having a structure as
follows (numbering according to the sequence of CONTIG-NAME_P1): a
sequence starting from any of amino acid numbers 49-x to 49 (for
example); and ending at any of amino acid numbers 50+((n-2)-x) (for
example), in which x varies from 0 to n-2. In this example, it
should also be read as including bridges in which n is any number
of amino acids between 10-50 amino acids in length. Furthermore,
the bridge polypeptide cannot extend beyond the sequence, so it
should be read such that 49-x (for example) is not less than 1, nor
50+((n-2)-x) (for example) greater than the total sequence
length.
[0430] In another embodiment, this invention provides antibodies
specifically recognizing the splice variants and polypeptide
fragments thereof of this invention. Preferably such antibodies
differentially recognize splice variants of the present invention
but do not recognize a corresponding known protein (such known
proteins are discussed with regard to their splice variants in the
Examples below).
[0431] In another embodiment, this invention provides an isolated
nucleic acid molecule encoding for a splice variant according to
the present invention, having a nucleotide sequence as set forth in
any one of the sequences listed herein, or a sequence complementary
thereto. In another embodiment, this invention provides an isolated
nucleic acid molecule, having a nucleotide sequence as set forth in
any one of the sequences listed herein, or a sequence complementary
thereto. In another embodiment, this invention provides an
oligonucleotide of at least about 12 nucleotides, specifically
hybridizable with the nucleic acid molecules of this invention. In
another embodiment, this invention provides vectors, cells,
liposomes and compositions comprising the isolated nucleic acids of
this invention.
[0432] In another embodiment, this invention provides a method for
detecting a splice variant according to the present invention in a
biological sample, comprising: contacting a biological sample with
an antibody specifically recognizing a splice variant according to
the present invention under conditions whereby the antibody
specifically interacts with the splice variant in the biological
sample but do not recognize known corresponding proteins (wherein
the known protein is discussed with regard to its splice variant(s)
in the Examples below), and detecting said interaction; wherein the
presence of an interaction correlates with the presence of a splice
variant in the biological sample.
[0433] In another embodiment, this invention provides a method for
detecting a splice variant nucleic acid sequences in a biological
sample, comprising: hybridizing the isolated nucleic acid molecules
or oligonucleotide fragments of at least about a minimum length to
a nucleic acid material of a biological sample and detecting a
hybridization complex; wherein the presence of a hybridization
complex correlates with the presence of a splice variant nucleic
acid sequence in the biological sample.
[0434] According to the present invention, the splice variants
described herein are non-limiting examples of markers for
diagnosing breast cancer and/or an indicative condition. Each
splice variant marker of the present invention can be used alone or
in combination, for various uses, including but not limited to,
prognosis, prediction, screening, early diagnosis, determination of
progression, therapy selection and treatment monitoring of breast
cancer and/or an indicative condition, including a transition from
an indicative condition to breast cancer.
[0435] According to optional but preferred embodiments of the
present invention, any marker according to the present invention
may optionally be used alone or combination. Such a combination may
optionally comprise a plurality of markers described herein,
optionally including any subcombination of markers, and/or a
combination featuring at least one other marker, for example a
known marker. Furthermore, such a combination may optionally and
preferably be used as described above with regard to determining a
ratio between a quantitative or semi-quantitative measurement of
any marker described herein to any other marker described herein,
and/or any other known marker, and/or any other marker. With regard
to such a ratio between any marker described herein (or a
combination thereof) and a known marker, more preferably the known
marker comprises the "known protein" as described in greater detail
below with regard to each cluster or gene.
[0436] According to other preferred embodiments of the present
invention, a splice variant protein or a fragment thereof, or a
splice variant nucleic acid sequence or a fragment thereof, may be
featured as a biomarker for detecting breast cancer and/or an
indicative condition, such that a biomarker may optionally comprise
any of the above.
[0437] According to still other preferred embodiments, the present
invention optionally and preferably encompasses any amino acid
sequence or fragment thereof encoded by a nucleic acid sequence
corresponding to a splice variant protein as described herein. Any
oligopeptide or peptide relating to such an amino acid sequence or
fragment thereof may optionally also (additionally or
alternatively) be used as a biomarker, including but not limited to
the unique amino acid sequences of these proteins that are depicted
as tails, heads, insertions, edges or bridges. The present
invention also optionally encompasses antibodies capable of
recognizing, and/or being elicited by, such oligopeptides or
peptides.
[0438] The present invention also optionally and preferably
encompasses any nucleic acid sequence or fragment thereof, or amino
acid sequence or fragment thereof, corresponding to a splice
variant of the present invention as described above, optionally for
any application.
[0439] Non-limiting examples of methods or assays are described
below.
[0440] The present invention also relates to kits based upon such
diagnostic methods or assays.
Nucleic Acid Sequences and Oligonucleotides
[0441] Various embodiments of the present invention encompass
nucleic acid sequences described hereinabove; fragments thereof,
sequences hybridizable therewith, sequences homologous thereto,
sequences encoding similar polypeptides with different codon usage,
altered sequences characterized by mutations, such as deletion,
insertion or substitution of one or more nucleotides, either
naturally occurring or artificially induced, either randomly or in
a targeted fashion.
[0442] The present invention encompasses nucleic acid sequences
described herein; fragments thereof, sequences hybridizable
therewith, sequences homologous thereto [e.g., at least 50%, at
least 55%, at least 60%, at least 65%, at least 70%, at least 75%,
at least 80%, at least 85%, least 95% or more say 100% identical to
the nucleic acid sequences set forth below], sequences encoding
similar polypeptides with different codon usage, altered sequences
characterized by mutations, such as deletion, insertion or
substitution of one or more nucleotides, either naturally occurring
or man induced, either randomly or in a targeted fashion. The
present invention also encompasses homologous nucleic acid
sequences (i.e., which form a part of a polynucleotide sequence of
the present invention) which include sequence regions unique to the
polynucleotides of the present invention.
[0443] In cases where the polynucleotide sequences of the present
invention encode previously unidentified polypeptides, the present
invention also encompasses novel polypeptides or portions thereof,
which are encoded by the isolated polynucleotide and respective
nucleic acid fragments thereof described hereinabove.
[0444] A "nucleic acid fragment" or an "oligonucleotide" or a
"polynucleotide" are used herein interchangeably to refer to a
polymer of nucleic acids. A polynucleotide sequence of the present
invention refers to a single or double stranded nucleic acid
sequences which is isolated and provided in the form of an RNA
sequence, a complementary polynucleotide sequence (cDNA), a genomic
polynucleotide sequence and/or a composite polynucleotide sequences
(e.g., a combination of the above).
[0445] As used herein the phrase "complementary polynucleotide
sequence" refers to a sequence, which results from reverse
transcription of messenger RNA using a reverse transcriptase or any
other RNA dependent DNA polymerase. Such a sequence can be
subsequently amplified in vivo or in vitro using a DNA dependent
DNA polymerase.
[0446] As used herein the phrase "genomic polynucleotide sequence"
refers to a sequence derived (isolated) from a chromosome and thus
it represents a contiguous portion of a chromosome.
[0447] As used herein the phrase "composite polynucleotide
sequence" refers to a sequence, which is composed of genomic and
cDNA sequences. A composite sequence can include some exonal
sequences required to encode the polypeptide of the present
invention, as well as some intronic sequences interposing
therebetween. The intronic sequences can be of any source,
including of other genes, and typically will include conserved
splicing signal sequences. Such intronic sequences may further
include cis acting expression regulatory elements.
[0448] Preferred embodiments of the present invention encompass
oligonucleotide probes.
[0449] An example of an oligonucleotide probe which can be utilized
by the present invention is a single stranded polynucleotide which
includes a sequence complementary to the unique sequence region of
any variant according to the present invention, including but not
limited to a nucleotide sequence coding for an amino sequence of a
bridge, tail, head and/or insertion according to the present
invention, and/or the equivalent portions of any nucleotide
sequence given herein (including but not limited to a nucleotide
sequence of a node, segment or amplicon described herein).
[0450] Alternatively, an oligonucleotide probe of the present
invention can be designed to hybridize with a nucleic acid sequence
encompassed by any of the above nucleic acid sequences,
particularly the portions specified above, including but not
limited to a nucleotide sequence coding for an amino sequence of a
bridge, tail, head and/or insertion according to the present
invention, and/or the equivalent portions of any nucleotide
sequence given herein (including but not limited to a nucleotide
sequence of a node, segment or amplicon described herein).
[0451] Oligonucleotides designed according to the teachings of the
present invention can be generated according to any oligonucleotide
synthesis method known in the art such as enzymatic synthesis or
solid phase synthesis. Equipment and reagents for executing
solid-phase synthesis are commercially available from, for example,
Applied Biosystems. Any other means for such synthesis may also be
employed; the actual synthesis of the oligonucleotides is well
within the capabilities of one skilled in the art and can be
accomplished via established methodologies as detailed in, for
example, "Molecular Cloning: A laboratory Manual" Sambrook et al.,
(1989); "Current Protocols in Molecular Biology" Volumes 1-111
Ausubel, R. M., ed. (1994); Ausubel et al., "Current Protocols in
Molecular Biology", John Wiley and Sons, Baltimore, Md. (1989);
Perbal, "A Practical Guide to Molecular Cloning", John Wiley &
Sons, New York (1988) and "Oligonucleotide Synthesis" Gait, M. J.,
ed. (1984) utilizing solid phase chemistry, e.g. cyanoethyl
phosphoramidite followed by deprotection, desalting and
purification by for example, an automated trityl-on method or
HPLC.
[0452] Oligonucleotides used according to this aspect of the
present invention are those having a length selected from a range
of about 10 to about 200 bases preferably about 15 to about 150
bases, more preferably about 20 to about 100 bases, most preferably
about 20 to about 50 bases. Preferably, the oligonucleotide of the
present invention features at least 17, at least 18, at least 19,
at least 20, at least 22, at least 25, at least 30 or at least 40,
bases specifically hybridizable with the biomarkers of the present
invention.
[0453] The oligonucleotides of the present invention may comprise
heterocylic nucleosides consisting of purines and the pyrimidines
bases, bonded in a 3' to 5' phosphodiester linkage.
[0454] Preferably used oligonucleotides are those modified at one
or more of the backbone, internucleoside linkages or bases, as is
broadly described hereinunder.
[0455] Specific examples of preferred oligonucleotides useful
according to this aspect of the present invention include
oligonucleotides containing modified backbones or non-natural
internucleoside linkages. Oligonucleotides having modified
backbones include those that retain a phosphorus atom in the
backbone, as disclosed in U.S. Pat. Nos. 4,469,863; 4,476,301;
5,023,243; 5,177,196; 5,188,897; 5,264,423; 5,276,019; 5,278,302;
5,286,717; 5,321,131; 5,399,676; 5,405,939; 5,453,496; 5,455,233;
5,466,677; 5,476,925; 5,519,126; 5,536,821; 5,541,306; 5,550,111;
5,563,253; 5,571,799; 5,587,361; and 5,625,050.
[0456] Preferred modified oligonucleotide backbones include, for
example, phosphorothioates, chiral phosphorothioates,
phosphorodithioates, phosphotriesters, aminoalkyl phosphotriesters,
methyl and other alkyl phosphonates including 3'-alkylene
phosphonates and chiral phosphonates, phosphinates,
phosphoramidates including 3'-amino phosphoramidate and
aminoalkylphosphoramidates, thionophosphoramidates,
thionoalkylphosphonates, thionoalkylphosphotriesters, and
boranophosphates having normal 3'-5' linkages, 2'-5' linked analogs
of these, and those having inverted polarity wherein the adjacent
pairs of nucleoside units are linked 3'-5' to 5'-3' or 2'-5' to
5'-2'. Various salts, mixed salts and free acid for also be
used.
[0457] Alternatively, modified oligonucleotide backbones that do
not include a phosphorus atom therein have backbones that are
formed by short chain alkyl or cycloalkyl internucleoside linkages,
mixed heteroatom and alkyl or cycloalkyl internucleoside linkages,
or one or more short chain heteroatomic or heterocyclic
internucleoside linkages. These include those having morpholino
linkages (formed in part from the sugar portion of a nucleoside);
siloxane backbones; sulfide, sulfoxide and sulfone backbones;
formacetyl and thioformacetyl backbones; methylene formacetyl and
thioformacetyl backbones; alkene containing backbones; sulfamate
backbones; methyleneimino and methylenehydrazino backbones;
sulfonate and sulfonamide backbones; amide backbones; and others
having mixed N, O, S and CH.sub.2 component parts, as disclosed in
U.S. Pat. Nos. 5,034,506; 5,166,315; 5,185,444; 5,214,134;
5,216,141; 5,235,033; 5,264,562; 5,264,564; 5,405,938; 5,434,257;
5,466,677; 5,470,967; 5,489,677; 5,541,307; 5,561,225; 5,596,086;
5,602,240; 5,610,289; 5,602,240; 5,608,046; 5,610,289; 5,618,704;
5,623,070; 5,663,312; 5,633,360; 5,677,437; and 5,677,439.
[0458] Other oligonucleotides which can be used according to the
present invention, are those modified in both sugar and the
internucleoside linkage, i.e., the backbone, of the nucleotide
units are replaced with novel groups. The base units are maintained
for complementation with the appropriate polynucleotide target. An
example for such an oligonucleotide mimetic, includes peptide
nucleic acid (PNA). U.S. patents that teach the preparation of PNA
compounds include, but are not limited to, U.S. Pat. Nos.
5,539,082; 5,714,331; and 5,719,262, each of which is herein
incorporated by reference. Other backbone modifications, which can
be used in the present invention are disclosed in U.S. Pat. No.
6,303,374.
[0459] Oligonucleotides of the present invention may also include
base modifications or substitutions. As used herein, "unmodified"
or "natural" bases include the purine bases adenine (A) and guanine
(G), and the pyrimidine bases thymine (T), cytosine (C) and uracil
(U). Modified bases include but are not limited to other synthetic
and natural bases such as 5-methylcytosine (5-me-C),
5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine,
6-methyl and other alkyl derivatives of adenine and guanine,
2-propyl and other alkyl derivatives of adenine and guanine,
2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and
cytosine, 5-propynyl uracil and cytosine, 6-azo uracil, cytosine
and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo,
8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted
adenines and guanines, 5-halo particularly 5-bromo,
5-trifluoromethyl and other 5-substituted uracils and cytosines,
7-methylguanine and 7-methyladenine, 8-azaguanine and 8-azaadenine,
7-deazaguanine and 7-deazaadenine and 3-deazaguanine and
3-deazaadenine. Further bases particularly useful for increasing
the binding affinity of the oligomeric compounds of the invention
include 5-substituted pyrimidines, 6-azapyrimidines and N-2, N-6
and O-6 substituted purines, including 2-aminopropyladenine,
5-propynyluracil and 5-propynylcytosine. 5-methylcytosine
substitutions have been shown to increase nucleic acid duplex
stability by 0.6-1.2.degree. C. and are presently preferred base
substitutions, even more particularly when combined with
2'-O-methoxyethyl sugar modifications.
[0460] Another modification of the oligonucleotides of the
invention involves chemically linking to the oligonucleotide one or
more moieties or conjugates, which enhance the activity, cellular
distribution or cellular uptake of the oligonucleotide. Such
moieties include but are not limited to lipid moieties such as a
cholesterol moiety, cholic acid, a thioether, e.g.,
hexyl-S-tritylthiol, a thiocholesterol, an aliphatic chain, e.g.,
dodecandiol or undecyl residues, a phospholipid, e.g.,
di-hexadecyl-rac-glycerol or triethylammonium
1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate, a polyamine or a
polyethylene glycol chain, or adamantane acetic acid, a palmityl
moiety, or an octadecylamine or hexylamino-carbonyl-oxycholesterol
moiety, as disclosed in U.S. Pat. No. 6,303,374.
[0461] It is not necessary for all positions in a given
oligonucleotide molecule to be uniformly modified, and in fact more
than one of the aforementioned modifications may be incorporated in
a single compound or even at a single nucleoside within an
oligonucleotide.
[0462] It will be appreciated that oligonucleotides of the present
invention may include further modifications for more efficient use
as diagnostic agents and/or to increase bioavailability,
therapeutic efficacy and reduce cytotoxicity.
[0463] To enable cellular expression of the polynucleotides of the
present invention, a nucleic acid construct according to the
present invention may be used, which includes at least a coding
region of one of the above nucleic acid sequences, and further
includes at least one cis acting regulatory element. As used
herein, the phrase "cis acting regulatory element" refers to a
polynucleotide sequence, preferably a promoter, which binds a trans
acting regulator and regulates the transcription of a coding
sequence located downstream thereto.
[0464] Any suitable promoter sequence can be used by the nucleic
acid construct of the present invention.
[0465] Preferably, the promoter utilized by the nucleic acid
construct of the present invention is active in the specific cell
population transformed. Examples of cell type-specific and/or
tissue-specific promoters include promoters such as albumin that is
liver specific, lymphoid specific promoters [Calame et al., (1988)
Adv. Immunol. 43:235-275]; in particular promoters of T-cell
receptors [Winoto et al., (1989) EMBO J. 8:729-733] and
immunoglobulins; [Banerji et al. (1983) Cell 33729-740],
neuron-specific promoters such as the neurofilament promoter [Byrne
et al. (1989) Proc. Natl. Acad. Sci. USA 86:5473-5477],
pancreas-specific promoters [Edlunch et al. (1985) Science
230:912-916] or mammary gland-specific promoters such as the milk
whey promoter (U.S. Pat. No. 4,873,316 and European Application
Publication No. 264,166). The nucleic acid construct of the present
invention can further include an enhancer, which can be adjacent or
distant to the promoter sequence and can function in up regulating
the transcription therefrom.
[0466] The nucleic acid construct of the present invention
preferably further includes an appropriate selectable marker and/or
an origin of replication. Preferably, the nucleic acid construct
utilized is a shuttle vector, which can propagate both in E. coli
(wherein the construct comprises an appropriate selectable marker
and origin of replication) and be compatible for propagation in
cells, or integration in a gene and a tissue of choice. The
construct according to the present invention can be, for example, a
plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an
artificial chromosome.
[0467] Examples of suitable constructs include, but are not limited
to, pcDNA3, pcDNA3.1 (+/), pGL3, PzeoSV2 (+/-), pDisplay,
pEF/myc/cyto, pCMV/myc/cyto each of which is commercially available
from Invitrogen Co. (invitrogen.com). Examples of retroviral vector
and packaging systems are those sold by Clontech, San Diego,
Calif., including Retro-X vectors pLNCX and pLXSN, which permit
cloning into multiple cloning sites and the transgene is
transcribed from CMV promoter. Vectors derived from Mo-MuLV are
also included such as pBabe, where the transgene will be
transcribed from the 5'LTR promoter.
[0468] Currently preferred in vivo nucleic acid transfer techniques
include transfection with viral or non-viral constructs, such as
adenovirus, lentivirus, Herpes simplex I virus, or adeno-associated
virus (AAV) and lipid-based systems. Useful lipids for
lipid-mediated transfer of the gene are, for example, DOTMA, DOPE,
and DC-Chol [Tonkinson et al., Cancer Investigation, 14(1): 54-65
(1996)]. The most preferred constructs for use in gene therapy are
viruses, most preferably adenoviruses, AAV, lentiviruses, or
retroviruses. A viral construct such as a retroviral construct
includes at least one transcriptional promoter/enhancer or
locus-defining element(s), or other elements that control gene
expression by other means such as alternate splicing, nuclear RNA
export, or post-translational modification of messenger. Such
vector constructs also include a packaging signal, long terminal
repeats (LTRs) or portions thereof, and positive and negative
strand primer binding sites appropriate to the virus used, unless
it is already present in the viral construct. In addition, such a
construct typically includes a signal sequence for secretion of the
peptide from a host cell in which it is placed. Preferably the
signal sequence for this purpose is a mammalian signal sequence or
the signal sequence of the polypeptide variants of the present
invention. Optionally, the construct may also include a signal that
directs polyadenylation, as well as one or more restriction sites
and a translation termination sequence. By way of example, such
constructs will typically include a 5' LTR, a tRNA binding site, a
packaging signal, an origin of second-strand DNA synthesis, and a
3' LTR or a portion thereof. Other vectors can be used that are
non-viral, such as cationic lipids, polylysine, and dendrimers.
Hybridization Assays
[0469] Detection of a nucleic acid of interest in a biological
sample may optionally be effected by hybridization-based assays
using an oligonucleotide probe (non-limiting examples of probes
according to the present invention were previously described).
[0470] Traditional hybridization assays include PCR, RT-PCR,
Real-time PCR, RNase protection, in-situ hybridization, primer
extension, Southern blots (DNA detection), dot or slot blots (DNA,
RNA), and Northern blots (RNA detection) (NAT type assays are
described in greater detail below). More recently, PNAs have been
described (Nielsen et al. 1999, Current Opin. Biotechnol.
10:71-75). Other detection methods include kits containing probes
on a dipstick setup and the like.
[0471] Hybridization based assays which allow the detection of a
variant of interest (i.e., DNA or RNA) in a biological sample rely
on the use of oligonucleotides which can be 10,15, 20, or 30 to 100
nucleotides long preferably from 10 to 50, more preferably from 40
to 50 nucleotides long.
[0472] Thus, the isolated polynucleotides (oligonucleotides) of the
present invention are preferably hybridizable with any of the
herein described nucleic acid sequences under moderate to stringent
hybridization conditions.
[0473] Moderate to stringent hybridization conditions are
characterized by a hybridization solution such as containing 10%
dextrane sulfate, 1 M NaCl, 1% SDS and 5.times.10.sup.6 cpm
.sup.32P labeled probe, at 65.degree. C., with a final wash
solution of 0.2.times.SSC and 0.1% SDS and final wash at 65.degree.
C and whereas moderate hybridization is effected using a
hybridization solution containing 10% dextrane sulfate, 1 M NaCl,
1% SDS and 5.times.10.sup.6 cpm .sup.32p labeled probe, at
65.degree. C, with a final wash solution of 1.times.SSC and 0.1%
SDS and final wash at 50.degree. C.
[0474] More generally, hybridization of short nucleic acids (below
200 bp in length, e.g. 17-40 bp in length) can be effected using
the following exemplary hybridization protocols which can be
modified according to the desired stringency; (i) hybridization
solution of 6.times.SSC and 1% SDS or 3 M TMACl, 0.01 M sodium
phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS, 100 .mu.g/ml
denatured salmon sperm DNA and 0.1% nonfat dried milk,
hybridization temperature of 1-1.5.degree. C. below the T.sub.m,
final wash solution of 3 M TMACl, 0.01 M sodium phosphate (pH 6.8),
1 mM EDTA (pH 7.6), 0.5% SDS at 1-1.5.degree. C. below the T.sub.m;
(ii) hybridization solution of 6.times.SSC and 0.1% SDS or 3 M
TMACl, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH 7.6), 0.5%
SDS, 100.mu.g/ml denatured salmon sperm DNA and 0.1% nonfat dried
milk, hybridization temperature of 2-2.5.degree. C. below the
T.sub.m, final wash solution of 3 M TMACl, 0.01 M sodium phosphate
(pH 6.8), 1 mM EDTA (pH 7.6), 0.5% SDS at 1-1.5.degree. C. below
the T.sub.m, final wash solution of 6.times.SSC, and final wash at
22.degree. C.; (iii) hybridization solution of 6.times.SSC and 1%
SDS or 3 M TMACl, 0.01 M sodium phosphate (pH 6.8), 1 mM EDTA (pH
7.6), 0.5% SDS, 100 mu.g/ml denatured salmon sperm DNA and 0.1%
nonfat dried milk, hybridization temperature.
[0475] The detection of hybrid duplexes can be carried out by a
number of methods. Typically, hybridization duplexes are separated
from unhybridized nucleic acids and the labels bound to the
duplexes are then detected. Such labels refer to radioactive,
fluorescent, biological or enzymatic tags or labels of standard use
in the art. A label can be conjugated to either the oligonucleotide
probes or the nucleic acids derived from the biological sample.
[0476] Probes can be labeled according to numerous well known
methods. Non-limiting examples of radioactive labels include 3H,
14C, 32P, and 35S. Non-limiting examples of detectable markers
include ligands, fluorophores, chemiluminescent agents, enzymes,
and antibodies. Other detectable markers for use with probes, which
can enable an increase in sensitivity of the method of the
invention, include biotin and radio-nucleotides. It will become
evident to the person of ordinary skill that the choice of a
particular label dictates the manner in which it is bound to the
probe.
[0477] For example, oligonucleotides of the present invention can
be labeled subsequent to synthesis, by incorporating biotinylated
dNTPs or rNTP, or some similar means (e.g., photo-cross-linking a
psoralen derivative of biotin to RNAs), followed by addition of
labeled streptavidin (e.g., phycoerythrin-conjugated streptavidin)
or the equivalent. Alternatively, when fluorescently-labeled
oligonucleotide probes are used, fluorescein, lissamine,
phycoerythrin, rhodamine (Perkin Elmer Cetus), Cy2, Cy3, Cy3.5,
Cy5, Cy5.5, Cy7, Fluor X (Amersham) and others [e.g., Kricka et al.
(1992), Academic Press San Diego, Calif.] can be attached to the
oligonucleotides.
[0478] Those skilled in the art will appreciate that wash steps may
be employed to wash away excess target DNA or probe as well as
unbound conjugate. Further, standard heterogeneous assay formats
are suitable for detecting the hybrids using the labels present on
the oligonucleotide primers and probes.
[0479] It will be appreciated that a variety of controls may be
usefully employed to improve accuracy of hybridization assays. For
instance, samples may be hybridized to an irrelevant probe and
treated with RNAse A prior to hybridization, to assess false
hybridization.
[0480] Although the present invention is not specifically dependent
on the use of a label for the detection of a particular nucleic
acid sequence, such a label might be beneficial, by increasing the
sensitivity of the detection. Furthermore, it enables automation.
Probes can be labeled according to numerous well known methods.
[0481] As commonly known, radioactive nucleotides can be
incorporated into probes of the invention by several methods.
Non-limiting examples of radioactive labels include .sup.3H,
sup.14C, .sup.32P, and .sup.35S.
[0482] Those skilled in the art will appreciate that wash steps may
be employed to wash away excess target DNA or probe as well as
unbound conjugate. Further, standard heterogeneous assay formats
are suitable for detecting the hybrids using the labels present on
the oligonucleotide primers and probes.
[0483] It will be appreciated that a variety of controls may be
usefully employed to improve accuracy of hybridization assays.
[0484] Probes of the invention can be utilized with naturally
occurring sugar-phosphate backbones as well as modified backbones
including phosphorothioates, dithionates, alkyl phosphonates and
a-nucleotides and the like. Probes of the invention can be
constructed of either ribonucleic acid (RNA) or deoxyribonucleic
acid (DNA), and preferably of DNA.
NAT Assays
[0485] Detection of a nucleic acid of interest in a biological
sample may also optionally be effected by NAT-based assays, which
involve nucleic acid amplification technology, such as PCR for
example (or variations thereof such as real-time PCR for
example).
[0486] As used herein, a "primer" defines an oligonucleotide which
is capable of annealing to (hybridizing with) a target sequence,
thereby creating a double stranded region which can serve as an
initiation point for DNA synthesis under suitable conditions.
[0487] Amplification of a selected, or target, nucleic acid
sequence may be carried out by a number of suitable methods. See
generally Kwoh et al., 1990, Am. Biotechnol. Lab. 8:14 Numerous
amplification techniques have been described and can be readily
adapted to suit particular needs of a person of ordinary skill.
Non-limiting examples of amplification techniques include
polymerase chain reaction (PCR), ligase chain reaction (LCR),
strand displacement amplification (SDA), transcription-based
amplification, the q3 replicase system and NASBA (Kwoh et al.,
1989, Proc. Natl. Acad. Sci. USA 86, 1173-1177; Lizardi et al.,
1988, BioTechnology 6:1197-1202; Malek et al., 1994, Methods Mol.
Biol., 28:253-260; and Sambrook et al., 1989, supra).
[0488] The terminology "amplification pair" (or "primer pair")
refers herein to a pair of oligonucleotides (oligos) of the present
invention, which are selected to be used together in amplifying a
selected nucleic acid sequence by one of a number of types of
amplification processes, preferably a polymerase chain reaction.
Other types of amplification processes include ligase chain
reaction, strand displacement amplification, or nucleic acid
sequence-based amplification, as explained in greater detail below.
As commonly known in the art, the oligos are designed to bind to a
complementary sequence under selected conditions.
[0489] In one particular embodiment, amplification of a nucleic
acid sample from a patient is amplified under conditions which
favor the amplification of the most abundant differentially
expressed nucleic acid. In one preferred embodiment, RT-PCR is
carried out on an mRNA sample from a patient under conditions which
favor the amplification of the most abundant mRNA. In another
preferred embodiment, the amplification of the differentially
expressed nucleic acids is carried out simultaneously. It will be
realized by a person skilled in the art that such methods could be
adapted for the detection of differentially expressed proteins
instead of differentially expressed nucleic acid sequences.
[0490] The nucleic acid (i.e. DNA or RNA) for practicing the
present invention may be obtained according to well known
methods.
[0491] Oligonucleotide primers of the present invention may be of
any suitable length, depending on the particular assay format and
the particular needs and targeted genomes employed. Optionally, the
oligonucleotide primers are at least 12 nucleotides in length,
preferably between 15 and 24 molecules, and they may be adapted to
be especially suited to a chosen nucleic acid amplification system.
As commonly known in the art, the oligonucleotide primers can be
designed by taking into consideration the melting point of
hybridization thereof with its targeted sequence (Sambrook et al.,
1989, Molecular Cloning--A Laboratory Manual, 2nd Edition, CSH
Laboratories; Ausubel et al., 1989, in Current Protocols in
Molecular Biology, John Wiley & Sons Inc., N.Y.).
[0492] It will be appreciated that antisense oligonucleotides may
be employed to quantify expression of a splice isoform of interest.
Such detection is effected at the pre-mRNA level. Essentially the
ability to quantitate transcription from a splice site of interest
can be effected based on splice site accessibility.
Oligonucleotides may compete with splicing factors for the splice
site sequences. Thus, low activity of the antisense oligonucleotide
is indicative of splicing activity.
[0493] The polymerase chain reaction and other nucleic acid
amplification reactions are well known in the art (various
non-limiting examples of these reactions are described in greater
detail below). The pair of oligonucleotides according to this
aspect of the present invention are preferably selected to have
compatible melting temperatures (Tm), e.g., melting temperatures
which differ by less than that 7.degree. C., preferably less than
5.degree. C., more preferably less than 4.degree. C., most
preferably less than 3.degree. C., ideally between 3.degree. C. and
0.degree. C.
[0494] Polymerase Chain Reaction (PCR): The polymerase chain
reaction (PCR), as described in U.S. Pat. Nos. 4,683,195 and
4,683,202 to Mullis and Mullis et al., is a method of increasing
the concentration of a segment of target sequence in a mixture of
genomic DNA without cloning or purification. This technology
provides one approach to the problems of low target sequence
concentration. PCR can be used to directly increase the
concentration of the target to an easily detectable level. This
process for amplifying the target sequence involves the
introduction of a molar excess of two oligonucleotide primers which
are complementary to their respective strands of the
double-stranded target sequence to the DNA mixture containing the
desired target sequence. The mixture is denatured and then allowed
to hybridize. Following hybridization, the primers are extended
with polymerase so as to form complementary strands. The steps of
denaturation, hybridization (annealing), and polymerase extension
(elongation) can be repeated as often as needed, in order to obtain
relatively high concentrations of a segment of the desired target
sequence.
[0495] The length of the segment of the desired target sequence is
determined by the relative positions of the primers with respect to
each other, and, therefore, this length is a controllable
parameter. Because the desired segments of the target sequence
become the dominant sequences (in terms of concentration) in the
mixture, they are said to be "PCR-amplified."
[0496] Ligase Chain Reaction (LCR or LAR): The ligase chain
reaction [LCR; sometimes referred to as "Ligase Amplification
Reaction" (LAR)] has developed into a well-recognized alternative
method of amplifying nucleic acids. In LCR, four oligonucleotides,
two adjacent oligonucleotides which uniquely hybridize to one
strand of target DNA, and a complementary set of adjacent
oligonucleotides, which hybridize to the opposite strand are mixed
and DNA ligase is added to the mixture. Provided that there is
complete complementarity at the junction, ligase will covalently
link each set of hybridized molecules. Importantly, in LCR, two
probes are ligated together only when they base-pair with sequences
in the target sample, without gaps or mismatches. Repeated cycles
of denaturation, and ligation amplify a short segment of DNA. LCR
has also been used in combination with PCR to achieve enhanced
detection of single-base changes: see for example Segev, PCT
Publication No. WO9001069 A1 (1990). However, because the four
oligonucleotides used in this assay can pair to form two short
ligatable fragments, there is the potential for the generation of
target-independent background signal. The use of LCR for mutant
screening is limited to the examination of specific nucleic acid
positions.
[0497] Self-Sustained Synthetic Reaction (3SR/NASBA): The
self-sustained sequence replication reaction (3SR) is a
transcription-based in vitro amplification system that can
exponentially amplify RNA sequences at a uniform temperature. The
amplified RNA can then be utilized for mutation detection. In this
method, an oligonucleotide primer is used to add a phage RNA
polymerase promoter to the 5' end of the sequence of interest. In a
cocktail of enzymes and substrates that includes a second primer,
reverse transcriptase, RNase H, RNA polymerase and ribo- and
deoxyribonucleoside triphosphates, the target sequence undergoes
repeated rounds of transcription, cDNA synthesis and second-strand
synthesis to amplify the area of interest. The use of 3SR to detect
mutations is kinetically limited to screening small segments of DNA
(e.g., 200-300 base pairs).
[0498] Q-Beta (Q.beta.) Replicase: In this method, a probe which
recognizes the sequence of interest is attached to the replicatable
RNA template for Q.beta. replicase. A previously identified major
problem with false positives resulting from the replication of
unhybridized probes has been addressed through use of a
sequence-specific ligation step. However, available thermostable
DNA ligases are not effective on this RNA substrate, so the
ligation must be performed by T4 DNA ligase at low temperatures (37
degrees C.). This prevents the use of high temperature as a means
of achieving specificity as in the LCR, the ligation event can be
used to detect a mutation at the junction site, but not
elsewhere.
[0499] A successful diagnostic method must be very specific. A
straight-forward method of controlling the specificity of nucleic
acid hybridization is by controlling the temperature of the
reaction. While the 3SR/NASBA, and Q.beta. systems are all able to
generate a large quantity of signal, one or more of the enzymes
involved in each cannot be used at high temperature (i.e., >55
degrees C.). Therefore the reaction temperatures cannot be raised
to prevent non-specific hybridization of the probes. If probes are
shortened in order to make them melt more easily at low
temperatures, the likelihood of having more than one perfect match
in a complex genome increases. For these reasons, PCR and LCR
currently dominate the research field in detection
technologies.
[0500] The basis of the amplification procedure in the PCR and LCR
is the fact that the products of one cycle become usable templates
in all subsequent cycles, consequently doubling the population with
each cycle. The final yield of any such doubling system can be
expressed as: (1+X).sup.n=y, where "X" is the mean efficiency
(percent copied in each cycle), "n" is the number of cycles, and
"y" is the overall efficiency, or yield of the reaction. If every
copy of a target DNA is utilized as a template in every cycle of a
polymerase chain reaction, then the mean efficiency is 100%. If 20
cycles of PCR are performed, then the yield will be 2.sup.20, or
1,048,576 copies of the starting material. If the reaction
conditions reduce the mean efficiency to 85%, then the yield in
those 20 cycles will be only 1.85.sup.20, or 220,513 copies of the
starting material. In other words, a PCR running at 85% efficiency
will yield only 21% as much final product, compared to a reaction
running at 100% efficiency. A reaction that is reduced to 50% mean
efficiency will yield less than 1% of the possible product.
[0501] In practice, routine polymerase chain reactions rarely
achieve the theoretical maximum yield, and PCRs are usually run for
more than 20 cycles to compensate for the lower yield. At 50% mean
efficiency, it would take 34 cycles to achieve the million-fold
amplification theoretically possible in 20, and at lower
efficiencies, the number of cycles required becomes prohibitive. In
addition, any background products that amplify with a better mean
efficiency than the intended target will become the dominant
products.
[0502] Also, many variables can influence the mean efficiency of
PCR, including target DNA length and secondary structure, primer
length and design, primer and dNTP concentrations, and buffer
composition, to name but a few. Contamination of the reaction with
exogenous DNA (e.g., DNA spilled onto lab surfaces) or
cross-contamination is also a major consideration. Reaction
conditions must be carefully optimized for each different primer
pair and target sequence, and the process can take days, even for
an experienced investigator. The laboriousness of this process,
including numerous technical considerations and other factors,
presents a significant drawback to using PCR in the clinical
setting. Indeed, PCR has yet to penetrate the clinical market in a
significant way. The same concerns arise with LCR, as LCR must also
be optimized to use different oligonucleotide sequences for each
target sequence. In addition, both methods require expensive
equipment, capable of precise temperature cycling.
[0503] Many applications of nucleic acid detection technologies,
such as in studies of allelic variation, involve not only detection
of a specific sequence in a complex background, but also the
discrimination between sequences with few, or single, nucleotide
differences. One method of the detection of allele-specific
variants by PCR is based upon the fact that it is difficult for Taq
polymerase to synthesize a DNA strand when there is a mismatch
between the template strand and the 3' end of the primer. An
allele-specific variant may be detected by the use of a primer that
is perfectly matched with only one of the possible alleles; the
mismatch to the other allele acts to prevent the extension of the
primer, thereby preventing the amplification of that sequence. This
method has a substantial limitation in that the base composition of
the mismatch influences the ability to prevent extension across the
mismatch, and certain mismatches do not prevent extension or have
only a minimal effect.
[0504] A similar 3'-mismatch strategy is used with greater effect
to prevent ligation in the LCR. Any mismatch effectively blocks the
action of the thermostable ligase, but LCR still has the drawback
of target-independent background ligation products initiating the
amplification. Moreover, the combination of PCR with subsequent LCR
to identify the nucleotides at individual positions is also a
clearly cumbersome proposition for the clinical laboratory.
[0505] The direct detection method according to various preferred
embodiments of the present invention may be, for example a cycling
probe reaction (CPR) or a branched DNA analysis.
[0506] When a sufficient amount of a nucleic acid to be detected is
available, there are advantages to detecting that sequence
directly, instead of making more copies of that target, (e.g., as
in PCR and LCR). Most notably, a method that does not amplify the
signal exponentially is more amenable to quantitative analysis.
Even if the signal is enhanced by attaching multiple dyes to a
single oligonucleotide, the correlation between the final signal
intensity and amount of target is direct. Such a system has an
additional advantage that the products of the reaction will not
themselves promote further reaction, so contamination of lab
surfaces by the products is not as much of a concern. Recently
devised techniques have sought to eliminate the use of
radioactivity and/or improve the sensitivity in automatable
formats. Two examples are the "Cycling Probe Reaction" (CPR), and
"Branched DNA" (bDNA).
[0507] Cycling probe reaction (CPR): The cycling probe reaction
(CPR), uses a long chimeric oligonucleotide in which a central
portion is made of RNA while the two termini are made of DNA.
Hybridization of the probe to a target DNA and exposure to a
thermostable RNase H causes the RNA portion to be digested. This
destabilizes the remaining DNA portions of the duplex, releasing
the remainder of the probe from the target DNA and allowing another
probe molecule to repeat the process. The signal, in the form of
cleaved probe molecules, accumulates at a linear rate. While the
repeating process increases the signal, the RNA portion of the
oligonucleotide is vulnerable to RNases that may carried through
sample preparation.
[0508] Branched DNA: Branched DNA (bDNA), involves oligonucleotides
with branched structures that allow each individual oligonucleotide
to carry 35 to 40 labels (e.g., alkaline phosphatase enzymes).
While this enhances the signal from a hybridization event, signal
from non-specific binding is similarly increased.
[0509] The detection of at least one sequence change according to
various preferred embodiments of the present invention may be
accomplished by, for example restriction fragment length
polymorphism (RFLP analysis), allele specific oligonucleotide (ASO)
analysis, Denaturing/Temperature Gradient Gel Electrophoresis
(DGGE/TGGE), Single-Strand Conformation Polymorphism (SSCP)
analysis or Dideoxy fingerprinting (ddF).
[0510] The demand for tests which allow the detection of specific
nucleic acid sequences and sequence changes is growing rapidly in
clinical diagnostics. As nucleic acid sequence data for genes from
humans and pathogenic organisms accumulates, the demand for fast,
cost-effective, and easy-to-use tests for as yet mutations within
specific sequences is rapidly increasing.
[0511] A handful of methods have been devised to scan nucleic acid
segments for mutations. One option is to determine the entire gene
sequence of each test sample (e.g., a bacterial isolate). For
sequences under approximately 600 nucleotides, this may be
accomplished using amplified material (e.g., PCR reaction
products). This avoids the time and expense associated with cloning
the segment of interest. However, specialized equipment and highly
trained personnel are required, and the method is too labor-intense
and expensive to be practical and effective in the clinical
setting.
[0512] In view of the difficulties associated with sequencing, a
given segment of nucleic acid may be characterized on several other
levels. At the lowest resolution, the size of the molecule can be
determined by electrophoresis by comparison to a known standard run
on the same gel. A more detailed picture of the molecule may be
achieved by cleavage with combinations of restriction enzymes prior
to electrophoresis, to allow construction of an ordered map. The
presence of specific sequences within the fragment can be detected
by hybridization of a labeled probe, or the precise nucleotide
sequence can be determined by partial chemical degradation or by
primer extension in the presence of chain-terminating nucleotide
analogs.
[0513] Restriction fragment length polymorphism (RFLP): For
detection of single-base differences between like sequences, the
requirements of the analysis are often at the highest level of
resolution. For cases in which the position of the nucleotide in
question is known in advance, several methods have been developed
for examining single base changes without direct sequencing. For
example, if a mutation of interest happens to fall within a
restriction recognition sequence, a change in the pattern of
digestion can be used as a diagnostic tool (e.g., restriction
fragment length polymorphism [RFLP] analysis).
[0514] Single point mutations have been also detected by the
creation or destruction of RFLPs. Mutations are detected and
localized by the presence and size of the RNA fragments generated
by cleavage at the mismatches. Single nucleotide mismatches in DNA
heteroduplexes are also recognized and cleaved by some chemicals,
providing an alternative strategy to detect single base
substitutions, generically named the "Mismatch Chemical Cleavage"
(MCC). However, this method requires the use of osmium tetroxide
and piperidine, two highly noxious chemicals which are not suited
for use in a clinical laboratory.
[0515] RFLP analysis suffers from low sensitivity and requires a
large amount of sample. When RFLP analysis is used for the
detection of point mutations, it is, by its nature, limited to the
detection of only those single base changes which fall within a
restriction sequence of a known restriction endonuclease. Moreover,
the majority of the available enzymes have 4 to 6 base-pair
recognition sequences, and cleave too frequently for many
large-scale DNA manipulations. Thus, it is applicable only in a
small fraction of cases, as most mutations do not fall within such
sites.
[0516] A handful of rare-cutting restriction enzymes with 8
base-pair specificities have been isolated and these are widely
used in genetic mapping, but these enzymes are few in number, are
limited to the recognition of G+C-rich sequences, and cleave at
sites that tend to be highly clustered. Recently, endonucleases
encoded by group I introns have been discovered that might have
greater than 12 base-pair specificity, but again, these are few in
number.
[0517] Allele specific oligonucleotide (ASO): If the change is not
in a recognition sequence, then allele-specific oligonucleotides
(ASOs), can be designed to hybridize in proximity to the mutated
nucleotide, such that a primer extension or ligation event can
bused as the indicator of a match or a mis-match. Hybridization
with radioactively labeled allelic specific oligonucleotides (ASO)
also has been applied to the detection of specific point mutations.
The method is based on the differences in the melting temperature
of short DNA fragments differing by a single nucleotide. Stringent
hybridization and washing conditions can differentiate between
mutant and wild-type alleles. The ASO approach applied to PCR
products also has been extensively utilized by various researchers
to detect and characterize point mutations in ras genes and gsp/gip
oncogenes. Because of the presence of various nucleotide changes in
multiple positions, the ASO method requires the use of many
oligonucleotides to cover all possible oncogenic mutations.
[0518] With either of the techniques described above (i.e., RFLP
and ASO), the precise location of the suspected mutation must be
known in advance of the test. That is to say, they are inapplicable
when one needs to detect the presence of a mutation within a gene
or sequence of interest.
[0519] Denaturing/Temperature Gradient Gel Electrophoresis
(DGGE/TGGE): Two other methods rely on detecting changes in
electrophoretic mobility in response to minor sequence changes. One
of these methods, termed "Denaturing Gradient Gel Electrophoresis"
(DGGE) is based on the observation that slightly different
sequences will display different patterns of local melting when
electrophoretically resolved on a gradient gel. In this manner,
variants can be distinguished, as differences in melting properties
of homoduplexes versus heteroduplexes differing in a single
nucleotide can detect the presence of mutations in the target
sequences because of the corresponding changes in their
electrophoretic mobilities. The fragments to be analyzed, usually
PCR products, are "clamped" at one end by a long stretch of G-C
base pairs (30-80) to allow complete denaturation of the sequence
of interest without complete dissociation of the strands. The
attachment of a GC "clamp" to the DNA fragments increases the
fraction of mutations that can be recognized by DGGE. Attaching a
GC clamp to one primer is critical to ensure that the amplified
sequence has a low dissociation temperature. Modifications of the
technique have been developed, using temperature gradients, and the
method can be also applied to RNA:RNA duplexes.
[0520] Limitations on the utility of DGGE include the requirement
that the denaturing conditions must be optimized for each type of
DNA to be tested. Furthermore, the method requires specialized
equipment to prepare the gels and maintain the needed high
temperatures during electrophoresis. The expense associated with
the synthesis of the clamping tail on one oligonucleotide for each
sequence to be tested is also a major consideration. In addition,
long running times are required for DGGE. The long running time of
DGGE was shortened in a modification of DGGE called constant
denaturant gel electrophoresis (CDGE). CDGE requires that gels be
performed under different denaturant conditions in order to reach
high efficiency for the detection of mutations.
[0521] A technique analogous to DGGE, termed temperature gradient
gel electrophoresis (TGGE), uses a thermal gradient rather than a
chemical denaturant gradient. TGGE requires the use of specialized
equipment which can generate a temperature gradient perpendicularly
oriented relative to the electrical field. TGGE can detect
mutations in relatively small fragments of DNA therefore scanning
of large gene segments requires the use of multiple PCR products
prior to running the gel.
[0522] Single-Strand Conformation Polymorphism (SSCP): Another
common method, called "Single-Strand Conformation Polymorphism"
(SSCP) was developed by Hayashi, Sekya and colleagues and is based
on the observation that single strands of nucleic acid can take on
characteristic conformations in non-denaturing conditions, and
these conformations influence electrophoretic mobility. The
complementary strands assume sufficiently different structures that
one strand may be resolved from the other. Changes in sequences
within the fragment will also change the conformation, consequently
altering the mobility and allowing this to be used as an assay for
sequence variations.
[0523] The SSCP process involves denaturing a DNA segment (e.g., a
PCR product) that is labeled on both strands, followed by slow
electrophoretic separation on a non-denaturing polyacrylamide gel,
so that intra-molecular interactions can form and not be disturbed
during the run. This technique is extremely sensitive to variations
in gel composition and temperature. A serious limitation of this
method is the relative difficulty encountered in comparing data
generated in different laboratories, under apparently similar
conditions.
[0524] Dideoxy fingerprinting (ddF): The dideoxy fingerprinting
(ddF) is another technique developed to scan genes for the presence
of mutations. The ddF technique combines components of Sanger
dideoxy sequencing with SSCP. A dideoxy sequencing reaction is
performed using one dideoxy terminator and then the reaction
products are electrophoresed on nondenaturing polyacrylamide gels
to detect alterations in mobility of the termination segments as in
SSCP analysis. While ddF is an improvement over SSCP in terms of
increased sensitivity, ddF requires the use of expensive
dideoxynucleotides and this technique is still limited to the
analysis of fragments of the size suitable for SSCP (i.e.,
fragments of 200-300 bases for optimal detection of mutations).
[0525] In addition to the above limitations, all of these methods
are limited as to the size of the nucleic acid fragment that can be
analyzed. For the direct sequencing approach, sequences of greater
than 600 base pairs require cloning, with the consequent delays and
expense of either deletion sub-cloning or primer walking, in order
to cover the entire fragment. SSCP and DGGE have even more severe
size limitations. Because of reduced sensitivity to sequence
changes, these methods are not considered suitable for larger
fragments. Although SSCP is reportedly able to detect 90% of
single-base substitutions within a 200 base-pair fragment, the
detection drops to less than 50% for 400 base pair fragments.
Similarly, the sensitivity of DGGE decreases as the length of the
fragment reaches 500 base-pairs. The ddF technique, as a
combination of direct sequencing and SSCP, is also limited by the
relatively small size of the DNA that can be screened.
[0526] According to a presently preferred embodiment of the present
invention the step of searching for any of the nucleic acid
sequences described here, in tumor cells or in cells derived from a
cancer patient is effected by any suitable technique, including,
but not limited to, nucleic acid sequencing, polymerase chain
reaction, ligase chain reaction, self-sustained synthetic reaction,
Q.beta.-Replicase, cycling probe reaction, branched DNA,
restriction fragment length polymorphism analysis, mismatch
chemical cleavage, heteroduplex analysis, allele-specific
oligonucleotides, denaturing gradient gel electrophoresis, constant
denaturant gel electrophoresis, temperature gradient gel
electrophoresis and dideoxy fingerprinting.
[0527] Detection may also optionally be performed with a chip or
other such device. The nucleic acid sample which includes the
candidate region to be analyzed is preferably isolated, amplified
and labeled with a reporter group. This reporter group can be a
fluorescent group such as phycoerythrin. The labeled nucleic acid
is then incubated with the probes immobilized on the chip using a
fluidics station. describe the fabrication of fluidics devices and
particularly microcapillary devices, in silicon and glass
substrates.
[0528] Once the reaction is completed, the chip is inserted into a
scanner and patterns of hybridization are detected. The
hybridization data is collected, as a signal emitted from the
reporter groups already incorporated into the nucleic acid, which
is now bound to the probes attached to the chip. Since the sequence
and position of each probe immobilized on the chip is known, the
identity of the nucleic acid hybridized to a given probe can be
determined.
[0529] It will be appreciated that when utilized along with
automated equipment, the above described detection methods can be
used to screen multiple samples for a disease and/or pathological
condition both rapidly and easily.
Amino Acid Sequences and Peptides
[0530] The terms "polypeptide," "peptide" and "protein" are used
interchangeably herein to refer to a polymer of amino acid
residues. The terms apply to amino acid polymers in which one or
more amino acid residue is an analog or mimetic of a corresponding
naturally occurring amino acid, as well as to naturally occurring
amino acid polymers. Polypeptides can be modified, e.g., by the
addition of carbohydrate residues to form glycoproteins. The terms
"polypeptide," "peptide" and "protein" include glycoproteins, as
well as non-glycoproteins.
[0531] Polypeptide products can be biochemically synthesized such
as by employing standard solid phase techniques. Such methods
include but are not limited to exclusive solid phase synthesis,
partial solid phase synthesis methods, fragment condensation,
classical solution synthesis. These methods are preferably used
when the peptide is relatively short (i.e., 10 kDa) and/or when it
cannot be produced by recombinant techniques (i.e., not encoded by
a nucleic acid sequence) and therefore involves different
chemistry.
[0532] Solid phase polypeptide synthesis procedures are well known
in the art and further described by John Morrow Stewart and Janis
Dillaha Young, Solid Phase Peptide Syntheses (2nd Ed., Pierce
Chemical Company, 1984).
[0533] Synthetic polypeptides can optionally be purified by
preparative high performance liquid chromatography [Creighton T.
(1983) Proteins, structures and molecular principles. WH Freeman
and Co. N.Y], after which their composition can be confirmed via
amino acid sequencing.
[0534] In cases where large amounts of a polypeptide are desired,
it can be generated using recombinant techniques such as described
by Bitter et al., (1987) Methods in Enzymol. 153:516-544, Studier
et al. (1990) Methods in Enzymol. 185:60-89, Brisson et al. (1984)
Nature 310:511-514, Takamatsu et al. (1987) EMBO J. 6:307-311,
Coruzzi et al. (1984) EMBO J. 3:1671-1680 and Brogli et al., (1984)
Science 224:838-843, Gurley et al. (1986) Mol. Cell. Biol.
6:559-565 and Weissbach & Weissbach, 1988, Methods for Plant
Molecular Biology, Academic Press, NY, Section VIII, pp
421-463.
[0535] The present invention also encompasses polypeptides encoded
by the polynucleotide sequences of the present invention, as well
as polypeptides according to the amino acid sequences described
herein. The present invention also encompasses homologues of these
polypeptides, such homologues can be at least 50%, at least 55%, at
least 60%, at least 65%, at least 70%, at least 75%, at least 80%,
at least 85%, at least 95% or more say 100% homologous to the amino
acid sequences set forth below, as can be determined using BlastP
software of the National Center of Biotechnology Information (NCBI)
using default parameters, optionally and preferably including the
following: filtering on (this option filters repetitive or
low-complexity sequences from the query using the Seg (protein)
program), scoring matrix is BLOSUM62 for proteins, word size is 3,
E value is 10, gap costs are 11, 1 (initialization and extension),
and number of alignments shown is 50. Finally, the present
invention also encompasses fragments of the above described
polypeptides and polypeptides having mutations, such as deletions,
insertions or substitutions of one or more amino acids, either
naturally occurring or artificially induced, either randomly or in
a targeted fashion. Homology/identity of nucleic acid sequences is
preferably determined by using BlastN software of the National
Center of Biotechnology Information (NCBI) using default
parameters, which preferably include using the DUST filter program,
and also preferably include having an E value of 10, filtering low
complexity sequences and a word size of 11.
[0536] It will be appreciated that peptides identified according
the present invention may be degradation products, synthetic
peptides or recombinant peptides as well as peptidomimetics,
typically, synthetic peptides and peptoids and semipeptoids which
are peptide analogs, which may have, for example, modifications
rendering the peptides more stable while in a body or more capable
of penetrating into cells. Such modifications include, but are not
limited to N terminus modification, C terminus modification,
peptide bond modification, including, but not limited to, CH2-NH,
CH2-S, CH2-S.dbd.O, O.dbd.C--NH, CH2-O, CH2-CH2, S.dbd.C--NH,
CH.dbd.CH or CF.dbd.CH, backbone modifications, and residue
modification. Methods for preparing peptidomimetic compounds are
well known in the art and are specified. Further details in this
respect are provided hereinunder.
[0537] Peptide bonds (--CO--NH--) within the peptide may be
substituted, for example, by N-methylated bonds (--N(CH3)-CO--),
ester bonds (--C(R)H--C--O--O--C(R)--N--), ketomethylen bonds
(--CO--CH2-), .alpha.-aza bonds (--NH--N(R)--CO--), wherein R is
any alkyl, e.g., methyl, carba bonds (--CH2-NH--), hydroxyethylene
bonds (--CH(OH)--CH2-), thioamide bonds (--CS--NH--), olefinic
double bonds (--CH.dbd.CH--), retro amide bonds (--NH--CO--),
peptide derivatives (--N(R)--CH2-CO--), wherein R is the "normal"
side chain, naturally presented on the carbon atom.
[0538] These modifications can occur at any of the bonds along the
peptide chain and even at several (2-3) at the same time.
[0539] Natural aromatic amino acids, Trp, Tyr and Phe, may be
substituted for synthetic non-natural acid such as Phenylglycine,
TIC, naphthylelanine (Nol), ring-methylated derivatives of Phe,
halogenated derivatives of Phe or o-methyl-Tyr.
[0540] In addition to the above, the peptides of the present
invention may also include one or more modified amino acids or one
or more non-amino acid monomers (e.g. fatty acids, complex
carbohydrates etc).
[0541] As used herein in the specification and in the claims
section below the term "amino acid" or "amino acids" is understood
to include the 20 naturally occurring amino acids; those amino
acids often modified post-translationally in vivo, including, for
example, hydroxyproline, phosphoserine and phosphothreonine; and
other unusual amino acids including, but not limited to,
2-aminoadipic acid, hydroxylysine, isodesmosine, nor-valine,
nor-leucine and ornithine. Furthermore, the term "amino acid"
includes both D- and L-amino acids.
[0542] Table 1 non-conventional or modified amino acids which can
be used with the present invention. TABLE-US-00087 TABLE 1
Non-conventional amino acid Code Non-conventional amino acid Code
.alpha.-aminobutyric acid Abu L-N-methylalanine Nmala
.alpha.-amino-.alpha.-methylbutyrate Mgabu L-N-methylarginine Nmarg
aminocyclopropane-Cpro L-N-methylasparagine Nmasn Carboxylate
L-N-methylaspartic acid Nmasp aminoisobutyric acid Aib
L-N-methylcysteine Nmcys aminonorbornyl-Norb L-N-methylglutamine
Nmgin Carboxylate L-N-methylglutamic acid Nmglu Cyclohexylalanine
Chexa L-N-methylhistidine Nmhis Cyclopentylalanine Cpen
L-N-methylisolleucine Nmile D-alanine Dal L-N-methylleucine Nmleu
D-arginine Darg L-N-methyllysine Nmlys D-aspartic acid Dasp
L-N-methylmethionine Nmmet D-cysteine Dcys L-N-methylnorleucine
Nmnle D-glutamine Dgln L-N-methylnorvaline Nmnva D-glutamic acid
Dglu L-N-methylornithine Nmorn D-histidine Dhis
L-N-methylphenylalanine Nmphe D-isoleucine Dile L-N-methylproline
Nmpro D-leucine Dleu L-N-methylserine Nmser D-lysine Dlys
L-N-methylthreonine Nmthr D-methionine Dmet L-N-methyltryptophan
Nmtrp D-ornithine Dorn L-N-methyltyrosine Nmtyr D-phenylalanine
Dphe L-N-methylvaline Nmval D-proline Dpro L-N-methylethylglycine
Nmetg D-serine Dser L-N-methyl-t-butylglycine Nmtbug D-threonine
Dthr L-norleucine Nle D-tryptophan Dtrp L-norvaline Nva D-tyrosine
Dtyr .alpha.-methyl-aminoisobutyrate Maib D-valine Dval
.alpha.-methyl-.gamma.-aminobutyrate Mgabu D-.alpha.-methylalanine
Dmala .alpha.-methylcyclohexylalanine Mchexa
D-.alpha.-methylarginine Dmarg .alpha.-methylcyclopentylalanine
Mcpen D-.alpha.-methylasparagine Dmasn
alpha.-methyl-.alpha.-napthylalanine Manap
D-.alpha.-methylaspartate Dmasp alpha.-methylpenicillamine Mpen
D-.alpha.-methylcysteine Dmcys N-(4-aminobutyl)glycine Nglu
D-.alpha.-methylglutamine Dmgln N-(2-aminoethyl)glycine Naeg
D-.alpha.-methylhistidine Dmhis N-(3-aminopropyl)glycine Norn
D-.alpha.-methylisoleucine Dmile N-amino-.alpha.-methylbutyrate
Nmaabu D-.alpha.-methylleucine Dmleu .alpha.-napthylalanine Anap
D-.alpha.-methyllysine Dmlys N-benzylglycine Nphe
D-.alpha.-methylmethionine Dmmet N-(2-carbamylethyl)glycine Ngln
D-.alpha.-methylornithine Dmorn N-(carbamylmethyl)glycine Nasn
D-.alpha.-methylphenylalanine Dmphe N-(2-carboxyethyl)glycine Nglu
D-.alpha.-methylproline Dmpro N-(carboxymethyl)glycine Nasp
D-.alpha.-methylserine Dmser N-cyclobutylglycine Ncbut
D-.alpha.-methylthreonine Dmthr N-cycloheptylglycine Nchep
D-.alpha.-methyltryptophan Dmtrp N-cyclohexylglycine Nchex
D-.alpha.-methyltyrosine Dmty N-cyclodecylglycine Ncdec
D-.alpha.-methylvaline Dmval N-cyclododeciglycine Ncdod
D-.alpha.-methylalnine Dnmala N-cyclooctylglycine Ncoct
D-.alpha.-methylarginine Dnmarg N-cyclopropylglycine Ncpro
D-.alpha.-methylasparagine Dnmasn N-cycloundecylglycine Ncund
D-.alpha.-methylasparatate Dnmasp N-(2,2-diphenylethyl)glycine Nbhm
D-.alpha.-methylcysteine Dnmcys N-(3,3-Nbhe diphenylpropyl)glycine
D-N-methylleucine Dnmleu N-(3-indolylyethyl)glycine Nhtrp
D-N-methyllysine Dnmlys N-methyl-.gamma.-aminobutyrate Nmgabu
N-Nmchexa D-N-methylmethionine Dnmmet methylcyclohexylalanine
D-N-methylornithine Dnmorn N-methylcyclopentylalanine Nmcpen
N-methylglycine Nala D-N-methylphenylalanine Dnmphe
N-methylaminoisobutyrate Nmaib D-N-methylproline Dnmpro
N-(1-methylpropyl)glycine Nile D-N-methylserine Dnmser
N-(2-methylpropyl)glycine Nile D-N-methylserine Dnmser
N-(2-methylpropyl)glycine Nleu D-N-methylthreonine Dnmthr
D-N-methyltryptophan Dnmtrp N-(1-methylethyl)glycine Nva
D-N-methyltyrosine Dnmtyr N-methyla-napthylalanine Nmanap
D-N-methylvaline Dnmval N-methylpenicillamine Nmpen
.gamma.-aminobutyric acid Gabu N-(p-hydroxyphenyl)glycine Nhtyr
L-t-butylglycine Tbug N-(thiomethyl)glycine Ncys L-ethylglycine Etg
penicillamine Pen L-homophenylalanine Hphe L-.alpha.-methylalanine
Mala L-.alpha.-methylarginine Marg L-.alpha.-methylasparagine Masn
L-.alpha.-methylaspartate Masp L-.alpha.-methyl-t-butylglycine
Mtbug L-.alpha.-methylcysteine Mcys L-methylethylglycine Metg
L-.alpha.-methylglutamine Mgln L-.alpha.-methylglutamate Mglu
L-.alpha.-methylhistidine Mhis L-.alpha.-methylhomo Mhphe
phenylalanine L-.alpha.-methylisoleucine Mile
N-(2-methylthioethyl)glycine Nmet D-N-methylglutamine Dnmgln
N-(3-Narg guanidinopropyl)glycine D-N-methylglutamate Dnmglu
N-(1-hydroxyethyl)glycine Nthr D-N-methylhistidine Dnmhis
N-(hydroxyethyl)glycine Nser D-N-methylisoleucine Dnmile
N-(imidazolylethyl)glycine Nhis D-N-methylleucine Dnmleu
N-(3-indolylyethyl)glycine Nhtrp D-N-methyllysine Dnmlys
N-methyl-.gamma.-aminobutyrate Nmgabu N-Nmchexa
D-N-methylmethionine Dnmmet methylcyclohexylalanine
D-N-methylornithine Dnmorn N-methylcyclopentylalanine Nmcpen
N-methylglycine Nala D-N-methylphenylalanine Dnmphe
N-methylaminoisobutyrate Nmaib D-N-methylproline Dnmpro
N-(1-methylpropyl)glycine Nile D-N-methylserine Dnmser
N-(2-methylpropyl)glycine Nleu D-N-methylthreonine Dnmthr
D-N-methyltryptophan Dnmtrp N-(1-methylethyl)glycine Nval
D-N-methyltyrosine Dnmtyr N-methyla-napthylalanine Nmanap
D-N-methylvaline Dnmval N-methylpenicillamine Nmpen
.gamma.-aminobutyric acid Gabu N-(p-hydroxyphenyl) glycine Nhtyr
L-t-butylglycine Tbug N-(thiomethyl)glycine Ncys L-ethylglycine Etg
penicillamine Pen L-homophenylalanine Hphe L-.alpha.-methylalanine
Mala L-.alpha.-methylarginine Marg L-.alpha.-methylasparagine Masn
L-.alpha.-methylaspartate Masp L-.alpha.-methyl-t-butylglycine
Mtbug L-.alpha.-methylcysteine Mcys L-methylethylglycine Metg
L-.alpha.-methylglutamine Mgln L-.alpha.-methylglutamate Mglu
L-.alpha.-methylhistidine Mhis L-.alpha.-Mhphe
methylhomophenylalanine L-.alpha.-methylisoleucine Mile
N-(2-methylthioethyl)glycine Nmet L-.alpha.-methylleucine Mleu
L-.alpha.-methyllysine Mlys L-.alpha.-methylmethionine Mmet
L-.alpha.-methylnorleucine Mnle L-.alpha.-methylnorvaline Mnva
L-.alpha.-methylornithine Morn L-.alpha.-methylphenylalanine Mphe
L-.alpha.-methylproline Mpro L-.alpha.-methylserine mser
L-.alpha.-methylthreonine Mthr L-.alpha.-methylvaline Mtrp
L-.alpha.-methyltyrosine Mtyr L-.alpha.-methylleucine Mval
L-N-Nmhphe Nnbhm methylhomophenylalanine N--(N-(2,2-diphenylethyl)
N--(N-(3,3-diphenylpropyl) carbamylmethyl-glycine Nnbhm
carbamylmethyl(1)glycine Nnbhe 1-carboxy-1-(2,2-diphenylethylamino)
Nmbc cyclopropane
[0543] Since the peptides of the present invention are preferably
utilized in diagnostics which require the peptides to be in soluble
form, the peptides of the present invention preferably include one
or more non-natural or natural polar amino acids, including but not
limited to serine and threonine which are capable of increasing
peptide solubility due to their hydroxyl-containing side chain.
[0544] The peptides of the present invention are preferably
utilized in a linear form, although it will be appreciated that in
cases where cyclicization does not severely interfere with peptide
characteristics, cyclic forms of the peptide can also be
utilized.
[0545] The peptides of present invention can be biochemically
synthesized such as by using standard solid phase techniques. These
methods include exclusive solid phase synthesis well known in the
art, partial solid phase synthesis methods, fragment condensation,
classical solution synthesis. These methods are preferably used
when the peptide is relatively short (i.e., 10 kDa) and/or when it
cannot be produced by recombinant techniques (i.e., not encoded by
a nucleic acid sequence) and therefore involves different
chemistry.
[0546] Synthetic peptides can be purified by preparative high
performance liquid chromatography and the composition of which can
be confirmed via amino acid sequencing.
[0547] In cases where large amounts of the peptides of the present
invention are desired, the peptides of the present invention can be
generated using recombinant techniques such as described by Bitter
et al., (1987) Methods in Enzymol. 153:516-544, Studier et al.
(1990) Methods in Enzymol. 185:60-89, Brisson et al. (1984) Nature
310:511-514, Takamatsu et al. (1987) EMBO J. 6:307-311, Coruzzi et
al. (1984) EMBO J. 3:1671-1680 and Brogli et al., (1984) Science
224:838-843, Gurley et al. (1986) Mol. Cell. Biol. 6:559-565 and
Weissbach & Weissbach, 1988, Methods for Plant Molecular
Biology, Academic Press, NY, Section VIII, pp 421-463 and also as
described above.
Antibodies
[0548] "Antibody" refers to a polypeptide ligand that is preferably
substantially encoded by an immunoglobulin gene or immunoglobulin
genes, or fragments thereof, which specifically binds and
recognizes an epitope (e.g., an antigen). The recognized
immunoglobulin genes include the kappa and lambda light chain
constant region genes, the alpha, gamma, delta, epsilon and mu
heavy chain constant region genes, and the myriad-immunoglobulin
variable region genes. Antibodies exist, e.g., as intact
immunoglobulins or as a number of well characterized fragments
produced by digestion with various peptidases. This includes, e.g.,
Fab' and F(ab)'.sub.2 fragments. The term "antibody," as used
herein, also includes antibody fragments either produced by the
modification of whole antibodies or those synthesized de novo using
recombinant DNA methodologies. It also includes polyclonal
antibodies, monoclonal antibodies, chimeric antibodies, humanized
antibodies, or single chain antibodies. "Fc" portion of an antibody
refers to that portion of an immunoglobulin heavy chain that
comprises one or more heavy chain constant region domains, CH1, CH2
and CH3, but does not include the heavy chain variable region.
[0549] The functional fragments of antibodies, such as Fab,
F(ab')2, and Fv that are capable of binding to macrophages, are
described as follows: (1) Fab, the fragment which contains a
monovalent antigen-binding fragment of an antibody molecule, can be
produced by digestion of whole antibody with the enzyme papain to
yield an intact light chain and a portion of one heavy chain; (2)
Fab', the fragment of an antibody molecule that can be obtained by
treating whole antibody with pepsin, followed by reduction, to
yield an intact light chain and a portion of the heavy chain; two
Fab' fragments are obtained per antibody molecule; (3)
(Fab').sub.2, the fragment of the antibody that can be obtained by
treating whole antibody with the enzyme pepsin without subsequent
reduction; F(ab')2 is a dimer of two Fab' fragments held together
by two disulfide bonds; (4) Fv, defined as a genetically engineered
fragment containing the variable region of the light chain and the
variable region of the heavy chain expressed as two chains; and (5)
Single chain antibody ("SCA"), a genetically engineered molecule
containing the variable region of the light chain and the variable
region of the heavy chain, linked by a suitable polypeptide linker
as a genetically fused single chain molecule.
[0550] Methods of producing polyclonal and monoclonal antibodies as
well as fragments thereof are well known in the art (See for
example, Harlow and Lane, Antibodies: A Laboratory Manual, Cold
Spring Harbor Laboratory, New York, 1988, incorporated herein by
reference).
[0551] Antibody fragments according to the present invention can be
prepared by proteolytic hydrolysis of the antibody or by expression
in E. coli or mammalian cells (e.g. Chinese hamster ovary cell
culture or other protein expression systems) of DNA encoding the
fragment. Antibody fragments can be obtained by pepsin or papain
digestion of whole antibodies by conventional methods. For example,
antibody fragments can be produced by enzymatic cleavage of
antibodies with pepsin to provide a 5S fragment denoted F(ab')2.
This fragment can be further cleaved using a thiol reducing agent,
and optionally a blocking group for the sulfhydryl groups resulting
from cleavage of disulfide linkages, to produce 3.5S Fab'
monovalent fragments. Alternatively, an enzymatic cleavage using
pepsin produces two monovalent Fab' fragments and an Fc fragment
directly. These methods are described, for example, by Goldenberg,
U.S. Pat. Nos. 4,036,945 and 4,331,647, and references contained
therein, which patents are hereby incorporated by reference in
their entirety. See also Porter, R. R. [Biochem. J. 73: 119-126
(1959)]. Other methods of cleaving antibodies, such as separation
of heavy chains to form monovalent light-heavy chain fragments,
further cleavage of fragments, or other enzymatic, chemical, or
genetic techniques may also be used, so long as the fragments bind
to the antigen that is recognized by the intact antibody.
[0552] Fv fragments comprise an association of VH and VL chains.
This association may be noncovalent, as described in Inbar et al.
[Proc. Nat'l Acad. Sci. USA 69:2659-62 (19720]. Alternatively, the
variable chains can be linked by an intermolecular disulfide bond
or cross-linked by chemicals such as glutaraldehyde. Preferably,
the Fv fragments comprise VH and VL chains connected by a peptide
linker. These single-chain antigen binding proteins (sFv) are
prepared by constructing a structural gene comprising DNA sequences
encoding the VH and VL domains connected by an oligonucleotide. The
structural gene is inserted into an expression vector, which is
subsequently introduced into a host cell such as E. coli. The
recombinant host cells synthesize a single polypeptide chain with a
linker peptide bridging the two V domains. Methods for producing
sFvs are described, for example, by [Whitlow and Filpula, Methods
2: 97-105 (1991); Bird et al., Science 242:423-426 (1988); Pack et
al., Bio/Technology 11:1271-77 (1993); and U.S. Pat. No. 4,946,778,
which is hereby incorporated by reference in its entirety.
[0553] Another form of an antibody fragment is a peptide coding for
a single complementarity-determining region (CDR). CDR peptides
("minimal recognition units") can be obtained by constructing genes
encoding the CDR of an antibody of interest. Such genes are
prepared, for example, by using the polymerase chain reaction to
synthesize the variable region from RNA of antibody-producing
cells. See, for example, Larrick and Fry [Methods, 2: 106-10
(1991)].
[0554] Humanized forms of non-human (e.g., murine) antibodies are
chimeric molecules of immunoglobulins, immunoglobulin chains or
fragments thereof (such as Fv, Fab, Fab', F(ab') or other
antigen-binding subsequences of antibodies) which contain minimal
sequence derived from non-human immunoglobulin. Humanized
antibodies include human immunoglobulins (recipient antibody) in
which residues from a complementary determining region (CDR) of the
recipient are replaced by residues from a CDR of a non-human
species (donor antibody) such as mouse, rat or rabbit having the
desired specificity, affinity and capacity. In some instances, Fv
framework residues of the human immunoglobulin are replaced by
corresponding non-human residues. Humanized antibodies may also
comprise residues which are found neither in the recipient antibody
nor in the imported CDR or framework sequences. In general, the
humanized antibody will comprise substantially all of at least one,
and typically two, variable domains, in which all or substantially
all of the CDR regions correspond to those of a non-human
immunoglobulin and all or substantially all of the FR regions are
those of a human immunoglobulin consensus sequence. The humanized
antibody optimally also will comprise at least a portion of an
immunoglobulin constant region (Fc), typically that of a human
immunoglobulin [Jones et al., Nature, 321:522-525 (1986); Riechmann
et al., Nature, 332:323-329 (1988); and Presta, Curr. Op. Struct.
Biol., 2:593-596 (1992)].
[0555] Methods for humanizing non-human antibodies are well known
in the art. Generally, a humanized antibody has one or more amino
acid residues introduced into it from a source which is non-human.
These non-human amino acid residues are often referred to as import
residues, which are typically taken from an import variable domain.
Humanization can be essentially performed following the method of
Winter and co-workers [Jones et al., Nature, 321:522-525 (1986);
Riechmann et al., Nature 332:323-327 (1988); Verhoeyen et al.,
Science, 239:1534-1536 (1988)], by substituting rodent CDRs or CDR
sequences for the corresponding sequences of a human antibody.
Accordingly, such humanized antibodies are chimeric antibodies
(U.S. Pat. No. 4,816,567), wherein substantially less than an
intact human variable domain has been substituted by the
corresponding sequence from a non-human species. In practice,
humanized antibodies are typically human antibodies in which some
CDR residues and possibly some FR residues are substituted by
residues from analogous sites in rodent antibodies.
[0556] Human antibodies can also be produced using various
techniques known in the art, including phage display libraries
[Hoogenboom and Winter, J. Mol. Biol., 227:381 (1991); Marks et
al., J. Mol. Biol., 222:581 (1991)]. The techniques of Cole et al.
and Boerner et al. are also available for the preparation of human
monoclonal antibodies (Cole et al., Monoclonal Antibodies and
Cancer Therapy, Alan R. Liss, p. 77 (1985) and Boerner et al., J.
Immunol., 147(1):86-95 (1991)]. Similarly, human antibodies can be
made by introduction of human immunoglobulin loci into transgenic
animals, e.g., mice in which the endogenous immunoglobulin genes
have been partially or completely inactivated. Upon challenge,
human antibody production is observed, which closely resembles that
seen in humans in all respects, including gene rearrangement,
assembly, and antibody repertoire. This approach is described, for
example, in U.S. Pat. Nos. 5,545,807; 5,545,806; 5,569,825;
5,625,126; 5,633,425; 5,661,016, and in the following scientific
publications: Marks et al., Bio/Technology 10,: 779-783 (1992);
Lonberg et al., Nature 368: 856-859 (1994); Morrison, Nature 368
812-13 (1994); Fishwild et al., Nature Biotechnology 14, 845-51
(1996); Neuberger, Nature Biotechnology 14: 826 (1996); and-
Lonberg and Huszar, Intern. Rev. Immunol. 13, 65-93 (1995).
[0557] Preferably, the antibody of this aspect of the present
invention specifically binds at least one epitope of the
polypeptide variants of the present invention. As used herein, the
term "epitope" refers to any antigenic determinant on an antigen to
which the paratope of an antibody binds.
[0558] Epitopic determinants usually consist of chemically active
surface groupings of molecules such as amino acids or carbohydrate
side chains and usually have specific three dimensional structural
characteristics, as well as specific charge characteristics.
[0559] Optionally, a unique epitope may be created in a variant due
to a change in one or more post-translational modifications,
including but not limited to glycosylation and/or phosphorylation,
as described below. Such a change may also cause a new epitope to
be created, for example through removal of glycosylation at a
particular site.
[0560] An epitope according to the present invention may also
optionally comprise part or all of a unique sequence portion of a
variant according to the present invention in combination with at
least one other portion of the variant which is not contiguous to
the unique sequence portion in the linear polypeptide itself, yet
which are able to form an epitope in combination. One or more
unique sequence portions may optionally combine with one or more
other non-contiguous portions of the variant (including a portion
which may have high homology to a portion of the known protein) to
form an epitope.
Immunoassays
[0561] In another embodiment of the present invention, an
immunoassay can be used to qualitatively or quantitatively detect
and analyze markers in a sample. This method comprises: providing
an antibody that specifically binds to a marker; contacting a
sample with the antibody; and detecting the presence of a complex
of the antibody bound to the marker in the sample.
[0562] To prepare an antibody that specifically binds to a marker,
purified protein markers can be used. Antibodies that specifically
bind to a protein marker can be prepared using any suitable methods
known in the art.
[0563] After the antibody is provided, a marker can be detected
and/or quantified using any of a number of well recognized
immunological binding assays. Useful assays include, for example,
an enzyme immune assay (EIA) such as enzyme-linked immunosorbent
assay (ELISA), a radioimmune assay (RIA), a Western blot assay, or
a slot blot assay see, e.g., U.S. Pat. Nos. 4,366,241; 4,376,110;
4,517,288; and 4,837,168). Generally, a sample obtained from a
subject can be contacted with the antibody that specifically binds
the marker.
[0564] Optionally, the antibody can be fixed to a solid support to
facilitate washing and subsequent isolation of the complex, prior
to contacting the antibody with a sample. Examples of solid
supports include but are not limited to glass or plastic in the
form of, e.g., a microtiter plate, a stick, a bead, or a microbead.
Antibodies can also be attached to a solid support.
[0565] After incubating the sample with antibodies, the mixture is
washed and the antibody-marker complex formed can be detected. This
can be accomplished by incubating the washed mixture with a
detection reagent. Alternatively, the marker in the sample can be
detected using an indirect assay, wherein, for example, a second,
labeled antibody is used to detect bound marker-specific antibody,
and/or in a competition or inhibition assay wherein, for example, a
monoclonal antibody which binds to a distinct epitope of the marker
are incubated simultaneously with the mixture.
[0566] Throughout the assays, incubation and/or washing steps may
be required after each combination of reagents. Incubation steps
can vary from about 5 seconds to several hours, preferably from
about 5 minutes to about 24 hours. However, the incubation time
will depend upon the assay format, marker, volume of solution,
concentrations and the like. Usually the assays will be carried out
at ambient temperature, although they can be conducted over a range
of temperatures, such as 10.degree. C. to 40.degree. C.
[0567] The immunoassay can be used to determine a test amount of a
marker in a sample from a subject. First, a test amount of a marker
in a sample can be detected using the immunoassay methods described
above. If a marker is present in the sample, it will form an
antibody-marker complex with an antibody that specifically binds
the marker under suitable incubation conditions described above.
The amount of an antibody-marker complex can optionally be
determined by comparing to a standard. As noted above, the test
amount of marker need not be measured in absolute units, as long as
the unit of measurement can be compared to a control amount and/or
signal.
[0568] Preferably used are antibodies which specifically interact
with the polypeptides of the present invention and not with wild
type proteins or other isoforms thereof, for example. Such
antibodies are directed, for example, to the unique sequence
portions of the polypeptide variants of the present invention,
including but not limited to bridges, heads, tails and insertions
described in greater detail below. Preferred embodiments of
antibodies according to the present invention are described in
greater detail with regard to the section entitled
"Antibodies".
[0569] Radio-immunoassay (RIA): In one version, this method
involves precipitation of the desired substrate and in the methods
detailed hereinbelow, with a specific antibody and radiolabelled
antibody binding protein (e.g., protein A labeled with I.sup.125)
immobilized on a precipitable carrier such as agarose beads. The
number of counts in the precipitated pellet is proportional to the
amount of substrate.
[0570] In an alternate version of the RIA, a labeled substrate and
an unlabelled antibody binding protein are employed. A sample
containing an unknown amount of substrate is added in varying
amounts. The decrease in precipitated counts from the labeled
substrate is proportional to the amount of substrate in the added
sample.
[0571] Enzyme linked immunosorbent assay (ELISA): This method
involves fixation of a sample (e.g., fixed cells or a proteinaceous
solution) containing a protein substrate to a surface such as a
well of a microtiter plate. A substrate specific antibody coupled
to an enzyme is applied and allowed to bind to the substrate.
Presence of the antibody is then detected and quantitated by a
calorimetric reaction employing the enzyme coupled to the antibody.
Enzymes commonly employed in this method include horseradish
peroxidase and alkaline phosphatase. If well calibrated and within
the linear range of response, the amount of substrate present in
the sample is proportional to the amount of color produced. A
substrate standard is generally employed to improve quantitative
accuracy.
[0572] Western blot: This method involves separation of a substrate
from other protein by means of an acrylamide gel followed by
transfer of the substrate to a membrane (e.g., nylon or PVDF).
Presence of the substrate is then detected by antibodies specific
to the substrate, which are in turn detected by antibody binding
reagents. Antibody binding reagents may be, for example, protein A,
or other antibodies. Antibody binding reagents may be radiolabelled
or enzyme linked as described hereinabove. Detection may be by
autoradiography, calorimetric reaction or chemiluminescence. This
method allows both quantitation of an amount of substrate and
determination of its identity by a relative position on the
membrane which is indicative of a migration distance in the
acrylamide gel during electrophoresis.
[0573] Immunohistochemical analysis: This method involves detection
of a substrate in situ in fixed cells by substrate specific
antibodies. The substrate specific antibodies may be enzyme linked
or linked to fluorophores. Detection is by microscopy and
subjective evaluation. If enzyme linked antibodies are employed, a
calorimetric reaction may be required.
[0574] Fluorescence activated cell sorting (FACS): This method
involves detection of a substrate in situ in cells by substrate
specific antibodies. The substrate specific antibodies are linked
to fluorophores. Detection is by means of a cell sorting machine
which reads the wavelength of light emitted from each cell as it
passes through a light beam. This method may employ two or more
antibodies simultaneously.
Radio-Imaging Methods
[0575] These methods include but are not limited to, positron
emission tomography (PET) single photon emission computed
tomography (SPECT). Both of these techniques are non-invasive, and
can be used to detect and/or measure a wide variety of tissue
events and/or functions, such as detecting cancerous cells for
example. Unlike PET, SPECT can optionally be used with two labels
simultaneously. SPECT has some other advantages as well, for
example with regard to cost and the types of labels that can be
used. For example, U.S. Pat. No. 6,696,686 describes the use of
SPECT for detection of breast cancer, and is hereby incorporated by
reference as if fully set forth herein.
Display Libraries
[0576] According to still another aspect of the present invention
there is provided a display library comprising a plurality of
display vehicles (such as phages, viruses or bacteria) each
displaying at least 6, at least 7, at least 8, at least 9, at least
10,10-15, 12-17, 15-20, 15-30 or 20-50 consecutive amino acids
derived from the polypeptide sequences of the present
invention.
[0577] Methods of constructing such display libraries are well
known in the art. Such methods are described in, for example, Young
A C, et al., "The three-dimensional structures of a polysaccharide
binding antibody to Cryptococcus neoformans and its complex with a
peptide from a phage display library: implications for the
identification of peptide mimotopes" J Mol Biol Dec. 12, 1997;
274(4):622-34; Giebel L B et al. "Screening of cyclic peptide phage
libraries identifies ligands that bind streptavidin with high
affinities" Biochemistry Nov. 28, 1995; 34(47):15430-5; Davies E L
et al., "Selection of specific phage-display antibodies using
libraries derived from chicken immunoglobulin genes" J Immunol
Methods Oct. 12, 1995; 186(1):125-35; Jones C R T al. "Current
trends in molecular recognition and bioseparation" J ChromatogrA
Jul. 14, 1995; 707(1):3-22; Deng S J et al. "Basis for selection of
improved carbohydrate-binding single-chain antibodies from
synthetic gene libraries" Proc Natl Acad Sci USA May 23, 1995;
92(11):4992-6; and Deng S J et al. "Selection of antibody
single-chain variable fragments with improved carbohydrate binding
by phage display" J Biol Chem. Apr. 1, 1994; 269(13):9533-8, which
are incorporated herein by reference.
[0578] The following sections relate to Candidate Marker Examples
(first section) and to Experimental Data for these Marker Examples
(second section).
Candidate Marker Examples Section
[0579] This Section relates to Examples of sequences according to
the present invention, including illustrative methods of selection
thereof.
Description of the Methodology Undertaken to Uncover the
Biomolecular Sequences of the Present Invention
[0580] Human ESTs and cDNAs were obtained from GenBank versions 136
(Jun. 15, 2003
ncbi.nih.gov/genbank/release.notes/gb136.release.notes); NCBI
genome assembly of April 2003; RefSeq sequences from June 2003;
Genbank version 139 (December 2003); Human Genome from NCBI (Build
34) (from October 2003); and RefSeq sequences from December 2003;
and from the LifeSeq library of Incyte Corporation (ESTs only;
Wilmington, Del., USA). With regard to GenBank sequences, the human
EST sequences from the EST (GBEST) section and the human mRNA
sequences from the primate (GBPRI) section were used; also the
human nucleotide RefSeq mRNA sequences were used (see for example
ncbi.nim.nih.gov/Genbank/GenbankOverview.html and for a reference
to the EST section, see ncbi.nlm.nih.gov/dbEST/; a general
reference to dbEST, the EST database in GenBank, may be found in
Boguski et al, Nat. Genet. 1993 August; 4(4):332-3; all of which
are hereby incorporated by reference as if fully set forth
herein).
[0581] Novel splice variants were predicted using the LEADS
clustering and assembly system as described in Sorek, R., Ast, G.
& Graur, D. Alu-containing exons are alternatively spliced.
Genome Res 12, 1060-7 (2002); U.S. Pat. No. 6,625,545; and U.S.
patent application Ser. No. 10/426,002, published as US20040101876
on May 27, 2004; all of which are hereby incorporated by reference
as if fully set forth herein. Briefly, the software cleans the
expressed sequences from repeats, vectors and immunoglobulins. It
then aligns the expressed sequences to the genome taking
alternatively splicing into account and clusters overlapping
expressed sequences into "clusters" that represent genes or partial
genes.
[0582] These were annotated using the GeneCarta (Compugen,
Tel-Aviv, Israel) platform. The GeneCarta platform includes a rich
pool of annotations, sequence information (particularly of spliced
sequences), chromosomal information, alignments, and additional
information such as SNPs, gene ontology terms, expression profiles,
functional analyses, detailed domain structures, known and
predicted proteins and detailed homology reports.
[0583] A brief explanation is provided with regard to the method of
selecting the candidates. However, it should noted that this
explanation is provided for descriptive purposes only, and is not
intended to be limiting in any way. The potential markers were
identified by a computational process that was designed to find
genes and/or their splice variants that are over-expressed in tumor
tissues, by using databases of expressed sequences. Various
parameters related to the information in the EST libraries,
determined according to a manual classification process, were used
to assist in locating genes and/or splice variants thereof that are
over-expressed in cancerous tissues. The detailed description of
the selection method is presented in Example 1 below. The cancer
biomarkers selection engine and the following wet validation stages
are schematically summarized in FIG. 1.
Example 1
Identification of Differentially Expressed Gene Products
Algorithm
[0584] In order to distinguish between differentially expressed
gene products and constitutively expressed genes (i.e., house
keeping genes) an algorithm based on an analysis of frequencies was
configured. A specific algorithm for identification of transcripts
over expressed in cancer is described hereinbelow.
[0585] Dry Analysis
[0586] Library annotation--EST libraries are manually classified
according to:
[0587] (i) Tissue origin
[0588] (ii) Biological source--Examples of frequently used
biological sources for construction of EST libraries include cancer
cell-lines; normal tissues; cancer tissues; fetal tissues; and
others such as normal cell lines and pools of normal cell-lines,
cancer cell-lines and combinations thereof. A specific description
of abbreviations used below with regard to these tissues/cell lines
etc is given above.
[0589] (iii) Protocol of library construction--various methods are
known in the art for library construction including normalized
library construction; non-normalized library construction;
subtracted libraries; ORESTES and others. It will be appreciated
that at times the protocol of library construction is not
indicated.
[0590] The following rules were followed:
[0591] EST libraries originating from identical biological samples
are considered as a single library.
[0592] EST libraries which included above-average levels of
contamination, such as DNA contamination for example, were
eliminated. The presence of such contamination was determined as
follows. For each library, the number of unspliced ESTs that are
not fully contained within other spliced sequences was counted. If
the percentage of such sequences (as compared to all other
sequences) was at least 4 standard deviations above the average for
all libraries being analyzed, this library was tagged as being
contaminated and was eliminated from further consideration in the
below analysis (see also Sorek, R. & Safer, H. M. A novel
algorithm for computational identification of contaminated EST
libraries. Nucleic Acids Res 31, 1067-74 (2003) for further
details).
[0593] Clusters (genes) having at least five sequences including at
least two sequences from the tissue of interest were analyzed.
Splice variants were identified by using the LEADS software package
as described above.
Example 2
Identification of Genes Over Expressed in Cancer
[0594] Two different scoring algorithms were developed.
[0595] Libraries score--candidate sequences which are supported by
a number of cancer libraries, are more likely to serve as specific
and effective diagnostic markers.
[0596] The basic algorithm--for each cluster the number of cancer
and normal libraries contributing sequences to the cluster was
counted. Fisher exact test was used to check if cancer libraries
are significantly over-represented in the cluster as compared to
the total number of cancer and normal libraries.
[0597] Library counting: Small libraries (e.g., less than 1000
sequences) were excluded from consideration unless they participate
in the cluster. For this reason, the total number of libraries is
actually adjusted for each cluster.
[0598] Clones no. score--Generally, when the number of ESTs is much
higher in the cancer libraries relative to the normal libraries it
might indicate actual over-expression.
[0599] The Algorithm--
[0600] Clone counting: For counting EST clones each library
protocol class was given a weight based on our belief of how much
the protocol reflects actual expression levels:
[0601] (i) non-normalized: 1
[0602] (ii) normalized: 0.2
[0603] (iii) all other classes: 0.1
[0604] Clones number score--The total weighted number of EST clones
from cancer libraries was compared to the EST clones from normal
libraries. To avoid cases where one library contributes to the
majority of the score, the contribution of the library that gives
most clones for a given cluster was limited to 2 clones.
[0605] The score was computed as c+1 C/n+1 N where:
[0606] c--weighted number of "cancer" clones in the cluster.
[0607] C--weighted number of clones in all "cancer" libraries.
[0608] n--weighted number of "normal" clones in the cluster.
[0609] N--weighted number of clones in all "normal" libraries.
[0610] Clones number score significance--Fisher exact test was used
to check if EST clones from cancer libraries are significantly
over-represented in the cluster as compared to the total number of
EST clones from cancer and normal libraries.
[0611] Two search approaches were used to find either general
cancer-specific candidates or tumor specific candidates.
[0612] Libraries/sequences originating from tumor tissues are
counted as well as libraries originating from cancer cell-lines
("normal" cell-lines were ignored).
[0613] Only libraries/sequences originating from tumor tissues are
counted
Example 3
Identification of Tissue Specific Genes
[0614] For detection of tissue specific clusters, tissue
libraries/sequences were compared to the total number of
libraries/sequences in cluster. Similar statistical tools to those
described in above were employed to identify tissue specific genes.
Tissue abbreviations are the same as for cancerous tissues, but are
indicated with the header "normal tissue".
[0615] The algorithm--for each tested tissue T and for each tested
cluster the following were examined:
[0616] 1. Each cluster includes at least 2 libraries from the
tissue T. At least 3 clones (weighed--as described above) from
tissue T in the cluster; and
[0617] 2. Clones from the tissue T are at least 40% from all the
clones participating in the tested cluster
[0618] Fisher exact test P-values were computed both for library
and weighted clone counts to check that the counts are
statistically significant.
Example 4
Identification of Splice Variants Expressed in Cancer of Clusters
which are not Over Expressed in Cancer
[0619] Cancer-Specific Splice Variants Containing a Unique Region
were Identified.
[0620] Identification of unique sequence regions in splice variants
A Region is defined as a group of adjacent exons that always appear
or do not appear together in each splice variant.
[0621] A "segment" (sometimes referred also as "seg" or "node") is
defined as the shortest contiguous transcribed region without known
splicing inside.
[0622] Only reliable ESTs were considered for region and segment
analysis. An EST was defined as unreliable if:
[0623] (i) Unspliced;
[0624] (ii) Not covered by RNA;
[0625] (iii) Not covered by spliced ESTs; and
[0626] (iv) Alignment to the genome ends in proximity of long
poly-A stretch or starts in proximity of long poly-T stretch.
[0627] Only reliable regions were selected for further scoring.
Unique sequence regions were considered reliable if:
[0628] (i) Aligned to the genome; and
[0629] (ii) Regions supported by more than 2 ESTs.
[0630] The algorithm
[0631] Each unique sequence region divides the set of transcripts
into 2 groups:
[0632] (i) Transcripts containing this region (group TA).
[0633] (ii) Transcripts not containing this region (group TB).
[0634] The set of EST clones of every cluster is divided into 3
groups:
[0635] (i) Supporting (originating from) transcripts of group TA
(S1).
[0636] (ii) Supporting transcripts of group TB (S2).
[0637] (iii) Supporting transcripts from both groups (S3).
[0638] Library and clones number scores described above were given
to S1 group.
[0639] Fisher Exact Test P-values were used to check if:
[0640] S1 is significantly enriched by cancer EST clones compared
to S2; and
[0641] S1 is significantly enriched by cancer EST clones compared
to cluster background (S1+S2+S3).
[0642] Identification of unique sequence regions and division of
the group of transcripts accordingly is illustrated in FIG. 2. Each
of these unique sequence regions corresponds to a segment, also
termed herein a "node".
[0643] Region 1: common to all transcripts, thus it is preferably
not considered for determining differential expression between
variants; Region 2: specific to Transcript 1; Region 3: specific to
Transcripts 2+3; Region 4: specific to Transcript 3; Region 5:
specific to Transcripts 1 and 2; Region 6: specific to Transcript
1.
Example 5
Identification of Cancer Specific Splice Variants of Genes Over
Expressed in Cancer
[0644] A search for EST supported (no mRNA) regions for genes
of:
[0645] (i) known cancer markers
[0646] (ii) Genes shown to be over-expressed in cancer in published
micro-array experiments.
[0647] Reliable EST supported-regions were defined as supported by
minimum of one of the following:
[0648] (i) 3 spliced ESTs; or
[0649] (ii) 2 spliced ESTs from 2 libraries;
[0650] (iii) 10 unspliced ESTs from 2 libraries, or
[0651] (iv) 3 libraries.
Actual Marker Examples
[0652] The following examples relate to specific actual marker
examples.
Experimental Examples Section
[0653] This Section relates to Examples describing experiments
involving these sequences, and illustrative, non-limiting examples
of methods, assays and uses thereof. The materials and experimental
procedures are explained first, as all experiments used them as a
basis for the work that was performed.
[0654] The markers of the present invention were tested with regard
to their expression in various cancerous and non-cancerous tissue
samples. A description of the samples used in the panel is provided
in Table 1 below. A description of the samples used in the normal
tissue panel is provided in Table 2 below. Tests were then
performed as described in the "Materials and Experimental
Procedures" section below. TABLE-US-00088 TABLE 1 Tissue samples in
testing panel sample sex/rename Lot no source pathology grade age
TNM stage 52-B-ILC G1 A605360 Biochain Invasive 1 F/60 Lobular
Carcinoma 51-B-IDC G1 A605361 Biochain IDC 1 F/79 6-A-IDC G1 7238T
ABS IDC 1 F/60 T2N0M0 stage 2A 7-A-IDC G2 7263T ABS IDC 2 F/43
T1N0M0 stage 1 12-A-IDC G2 1432T ABS IDC 2 F/46 T2N0M0 stage 2A
13-A-IDC G2A0133T ABS IDC 2 F/63 T2N1a Mx 14-A-IDC G2 A01 35T ABS
IDC 2 F/37 T2N2Mx 15-A-IDC G2 7259T ABS IDC 2 F/59 T3N1M0 stage 3A
16-A-IDC G2 490-4020032T ABS IDC 2 NA T3N1 Mx 17-A-IDC G2
490-4020036T ABS IDC 2-3 NAT3N1Mx 43-B-IDC G2 A609183 Biochain IDC
2 F/40 44-B-IDC G2 A609198 Biochain IDC 2 F/77 45-B-IDC G2 A609181
Biochain IDC 2 F/58 48-B-IDC G2 A609222 Biochain IDC 2 F/44
49-B-IDC G2 A609223 Biochain IDC 2 F/54 50-B-IDC G2 A609224
Biochain IDC 2 F/69 53-B-IDC G2 A605151 Biochain IDC 2 F/44
54-B-IDC G2 A605353 Biochain IDC 2 F/41 55-B-IDC G2 A609179
Biochain IDC 2 F/42 61-B-IDC G2 A610029 Biochain IDC 2 F/46
62-B-IDC G2A609194 Biochain IDC 2 F/51 47-B-IDC G2 A609221 Biochain
IDC 2 46-B-Carci G2 A609177 Biochain Carcinoma 2 F/48 26-A-IDC G3
7249T ABS IDC 3 F/60 T2N0M0 stage 2A 27-A-IDC G3 4907020072T ABS
IDC 3 NA T2N0Mx 42-A-IDC G3 6005020031T ABS IDC 3 NA T1cN0Mx
31-CG-IDC CG-154 Ichilov IDC NA 32-A-Muc 7116T ABS Mucinous F/54
T2N0M0 stage Carci carcinoma 2A 35-A-N M6 7238N ABS Normal F/60
matched to 6T 36-A-N M7 7263N ABS Normal F/43 matched to 7T 39-A-N
M15 7259N ABS Normal F/59 matched to 15T 40-A-N M12 1432N ABS
Normal F/46 matched to 12T 41-A-N M26 7249N ABS Normal F/60 matched
to 26T 56-B-N A609235 Biochain Normal F/59 PM 57-B-N A609233
Biochain Normal F/34 PM 58-B-N A609232 Biochain Normal F/65 PM
59-B-N A607155 Biochain Normal F/35 PM 60-B-N A609234 Biochain
Normal F/36 PM 63-Am-N 26486 Ambion Normal PS F/43 64-Am-N 23036
Ambion Normal F/57 PM 65-Am-N 31410 Ambion Normal F/63 PM 66-Am-N
36678 Ambion Normal F/45 PM 67-Am-N 073P01 0602086A Ambion Normal
F/64 PM
[0655] TABLE-US-00089 TABLE 2 Tissue samples in normal panel: Lot
no. Source Tissue Pathology Sex/Age 1-Am-Colon (C71) 071P10B Ambion
Colon PM F/43 2-B-Colon (C69) A411078 Biochain Colon PM-Pool of 10
M&F 3-Cl-Colon (C70) 1110101 Clontech Colon PM-Pool of 3
M&F 4-Am-Small Intestine 091 P0201A Ambion Small Intestine PM
M/75 5-B-Small Intestine A501158 Biochain Small Intestine PM M/63
6-B-Rectum A605138 Biochain Rectum PM M/25 7-B-Rectum A610297
Biochain Rectum PM M/24 8-B-Rectum A610298 Biochain Rectum PM M/27
9-Am-Stomach 110P04A Ambion Stomach PM M/16 10-B-Stomach A501159
Biochain Stomach PM M/24 11-B-Esophagus A603814 Biochain Esophagus
PM M/26 12-B-Esophagus A603813 Biochain Esophagus PM M/41
13-Am-Pancreas 071P25C Ambion Pancreas PM M/25 14-CG-Pancreas
CG-255-2 Ichilov Pancreas PM M/75 15-B-Lung A409363 Biochain Lung
PM F/26 16-Am-Lung (L93) 111P0103A Ambion Lung PM F/61 17-B-Lung
(L92) A503204 Biochain Lung PM M/28 18-Am-Ovary (O47) 061P43A
Ambion Ovary PM F/16 19-B-Ovary (O48) A504087 Biochain Ovary PM
F/51 20-B-Ovary (O46) A504086 Biochain Ovary PM F/41 21-Am-Cervix
101P0101A Ambion Cervix PM F/40 22-B-Cervix A408211 Biochain Cervix
PM F/36 23-B-Cervix A504089 Biochain Cervix PM-Pool of 5 M&F
24-B-Uterus A411074 Biochain Uterus PM-Pool of 10 M&F
25-B-Uterus A409248 Biochain Uterus PM F/43 26-B-Uterus A504090
Biochain Uterus PM-Pool of 5 M&F 27-B-Bladder A501157 Biochain
Bladder PM M/29 28-Am-Bladder 071 P02C Ambion Bladder PM M/20
29-B-Bladder A504088 Biochain Bladder PM-Pool of 5 M&F
30-Am-Placenta 021P33A Ambion Placenta PB F/33 31-B-Placenta
A410165 Biochain Placenta PB F/26 32-B-Placenta A411073 Biochain
Placenta PB-Pool of 5 M&F 33-B-Breast (B59) A607155 Biochain
Breast PM F/36 34-Am-Breast (B63) 26486 Ambion Breast PM F/43
35-Am-Breast (B64) 23036 Ambion Breast PM F/57 36-Cl-Prostate (P53)
1070317 Clontech Prostate PB-Pool of 47 M&F 37-Am-Prostate
(P42) 061P04A Ambion Prostate PM M/47 38-Am-Prostate (P59) 25955
Ambion Prostate PM M/62 39-Am-Testis 111P0104AAmbion Testis PM M/25
40-B-Testis A411147 Biochain Testis PM M/74 41-Cl-Testis 1110320
Clontech Testis PB-Pool of 45 M&F 42-CG-Adrenal CG-1 84-10
Ichilov Adrenal PM F/81 43-B-Adrenal A610374 Biochain Adrenal PM
F/83 44-B-Heart A411077 Biochain Heart PB-Pool of 5 M&F
45-CG-Heart CG-255-9 Ichilov Heart PM M/75 46-CG-Heart CG-227-1
Ichilov Heart PM F/36 47-Am-Liver 081P0101A Ambion Liver PM M/64
48-CG-Liver CG-93-3 Ichilov Liver PM F/19 49-CG-Liver CG-124-4
Ichilov Liver PM F/34 50-Cl-BM 1110932 Clontech Bone Marrow PM-Pool
of 8 M&F 51-CGEN-Blood WBC#5 CGEN Blood M 52-CGEN-Blood WBC#4
CGEN Blood M 53-CGEN-Blood WBC#3 CGEN Blood M 54-CG-Spleen CG-267
Ichilov Spleen PM F/25 55-CG-Spleen 111P01 06B Ambion Spleen PM
M/25 56-CG-Spleen A409246 Biochain Spleen PM F/12 56-CG-Thymus
CG-98-7 Ichilov Thymus PM F/28 58-Am-Thymus 101P0101A Ambion Thymus
PM M/1 4 59-B-Thymus A409278 Biochain Thymus PM M/28 60-B-Thyroid
A610287 Biochain Thyroid PM M/27 61-B-Thyroid A610286 Biochain
Thyroid PM M/24 62-CG-Thyroid CG-119-2 Ichilov Thyroid PM F/66
63-Cl-Salivary Gland 1070319 Clontech Salivary Gland PM-Pool of 24
M&F 64-Am-Kidney 111P0101B Ambion Kidney PM-Pool of 14 M&F
65-Cl-Kidney 1110970 Clontech Kidney PM-Pool of 14 M&F
66-B-Kidney A411080 Biochain Kidney PM-Pool of 5 M&F
67-CG-Cerebellum CG-1 83-5 Ichilov Cerebellum PM M/74
68-CG-Cerebellum CG-212-5 Ichilov Cerebellum PM M/54 69-B-Brain
A411322 Biochain Brain PM M/28 70-Cl-Brain 1120022 Clontech Brain
PM-Pool of 2 M&F 71-B-Brain A411079 Biochain Brain PM-Pool of 2
M&F 72-CG-Brain CG-151-1 Ichilov Brain PM F/86 73-Am-Skeletal
Muscle 101P013A Ambion Skeletal Muscle PM F/28 74-Cl-Skeletal
Muscle 1061038 Clontech Skeletal Muscle PM-Pool of 2 M&F
Materials and Experimental Procedures
[0656] RNApreparation--RNAwas obtained from Clontech (Franklin
Lakes, N.J. USA 07417, clontech.com), BioChain Inst. Inc. (Hayward,
Calif. 94545 USA biochain.com), ABS (Wilmington, Del. 19801, USA,
absbioreagents.com) or Ambion (Austin, Tex. 78744 USA, ambion.com).
Alternatively, RNA was generated from tissue samples using
TRI-Reagent (Molecular Research Center), according to
Manufacturer's instructions. Tissue and RNA samples were obtained
from patients or from postmortem. Total RNA samples were treated
with DNasel (Ambion) and purified using RNeasy columns
(Qiagen).
[0657] RT PCR--Purified RNA (1.mu.g) was mixed with 150 ng Random
Hexamer primers (Invitrogen) and 500.mu.M dNTP in a total volume of
15.6.mu.l. The mixture was incubated for 5 min at 65.degree. C. and
then quickly chilled on ice. Thereafter, 5.mu.l of 5.times.
SuperscriptII first strand buffer (Invitrogen), 2.4.mu.I 0.1M DTT
and 40 units RNasin (Promega) were added, and the mixture was
incubated for 10 min at 25.degree. C., followed by further
incubation at 42.degree. C. for 2 min. Then, 1.mu.l (200units) of
SuperscriptII (Invitrogen) was added and the reaction (final volume
of 25.mu.1) was incubated for 50 min at 42.degree. C. and then
inactivated at 70.degree. C. for 15 min. The resulting cDNA was
diluted 1:20 in TE buffer (10 mM Tris pH=8, 1 mM EDTA pH=8).
[0658] Real-Time RT-PCR analysis--cDNA (5.mu.l), prepared as
described above, was used as a template in Real-Time PCR reactions
using the SYBR Green I assay (PE Applied Biosystem) with specific
primers and UNG Enzyme (Eurogentech or ABI or Roche). The
amplification was effected as follows: 50.degree. C. for 2 min,
95.degree. C. for 10 min, and then 40 cycles of 95.degree. C. for
15 sec, followed by 60.degree. C. for 1 min. Detection was
performed by using the PE Applied Biosystem SDS 7000. The cycle in
which the reactions achieved a threshold level (Ct) of fluorescence
was registered and was used to calculate the relative transcript
quantity in the RT reactions. The relative quantity was calculated
using the equation Q=efficiencyl .sup.-Ct. The efficiency of the
PCR reaction was calculated from a standard curve, created by using
serial dilutions of several reverse transcription (RT) reactions.
To minimize inherent differences in the RT reaction, the resulting
relative quantities were normalized to the geometric mean of the
relative quantities of several housekeeping (HSKP) genes. Schematic
summary of quantitative real-time PCR analysis is presented in FIG.
3. As shown, the x-axis shows the cycle number. The
C.sub.T=Threshold Cycle point, which is the cycle that the
amplification curve crosses the fluorescence threshold that was set
in the experiment. This point is a calculated cycle number in which
PCR product signal is above the background level (passive dye ROX)
and still in the Geometric/Exponential phase (as shown, once the
level of fluorescence crosses the measurement threshold, it has a
geometrically increasing phase, during which measurements are most
accurate, followed by a linear phase and a plateau phase; for
quantitative measurements, the latter two phases do not provide
accurate measurements). The y-axis shows the normalized reporter
fluorescence. It should be noted that this type of analysis
provides relative quantification.
[0659] The sequences of the housekeeping genes measured in all the
examples on breast cancer panel were as follows: TABLE-US-00090
G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID NO: 918)) G6PD
Forward primer: (SEQ ID NO: 919) gaggccgtcaccaagaacat G6PD Reverse
primer: (SEQ ID NO: 920) ggacagccggtcagagctc G6PD-amplicon: (SEQ ID
NO: 921)
gaggccgtcaccaagaacattcacgagtcctgcatgagccagataggctggaaccgcatcatcgtggagaagc-
c-cttcgggagggacct gcagagctctgaccggctgtcc SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO: 922)) SDHA Forward primer: (SEQ ID
NO: 923) TGGGAACAAGAGGGCATCTG SDHA reverse primer: (SEQ ID NO: 924)
CCACCACTGCATCAAATTCATG SDHA-amplicon: (SEQ ID NO: 925)
TGGGAACAAGAGGGCATCTGCTAAAGTTTCAGATTCCATTTCTGCTCAGTATCCAGT
AGTGGATCATGAATTTGATGCAGTGGTGG PBGD (GenBank Accession No. BC019323,
(SEQ ID NO: 926)) PBGD Forward primer: (SEQ ID NO: 927)
TGAGAGTGATTCGCGTGGG PBGD Reverse primer: (SEQ ID NO: 928)
CCAGGGTACGAGGCTTTCAAT PBGD-amplicon: (SEQ ID NO: 929)
TGAGAGTGATTCGCGTGGGTACCCGCAAGAGCCAGCTTGCTCGCATACAGACGGAC
AGTGTGGTGGCAACATTGAAAGCCTCGTACCCTGG HPRT1 (GenBank Accession No.
NM.sub.--000194, (SEQ ID NO: 930)) HPRT1 Forward primer: (SEQ ID
NO: 931) TGACACTGGCAAAACAATGCA HPRT1 reverse primer: (SEQ ID NO:
932) GGTCCTTTTCACCAGCAAGCT HPRT1-amplicon: (SEQ ID NO: 933)
TGACACTGGCAAAACAATGCAGACTTTGCTTTCCTTGGTCAGGCAGTATAATCCAA
AGATGGTCAAGGTCGCAAGCTTGCTGGTGAAAAGGACC
[0660] The sequences of the housekeeping genes measured in all the
examples on normal tissue samples panel were as follows:
TABLE-US-00091 RPL19 (GenBank Accession No. NM.sub.--000981, (SEQ
ID NO: 934)) RPL19 Forward primer: (SEQ ID NO: 935)
TGGCAAGAAGAAGGTCTGGTTAG RPL19 reverse primer: (SEQ ID NO: 936)
TGATCAGCCCATCTTTGATGAG RPL19-amplicon: (SEQ ID NO: 937)
TGGCAAGAAGAAGGTCTGGTTAGACCCCAATGAGACCAATGAAATCGCCAATGCCA
ACTCCCGTCAGCAGATCCGGAAGCTCATCAAAGATGGGCTGATCA TATA box (GenBank
Accession No. NM.sub.--003194, (SEQ ID NO: 938)) TATA box Forward
primer: (SEQ ID NO: 939) CGGTTTGCTGCGGTAATCAT TATA box Reverse
primer: (SEQ ID NO: 940) TTTCTTGCTGCCAGTCTGGAC TATA box-amplicon:
(SEQ ID NO: 941)
CGGTTTGCTGCGGTAATCATGAGGATAAGAGAGCCACGAACCACGGCACTGATTTT
CAGTTCTGGGAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAACAGTCCAGACTG GCAGCAAGAAA
UBC (GenBank Accession No. BC000449 (SEQ ID NO: 942)) UBC Forward
primer: (SEQ ID NO: 943) ATTTGGGTCGCGGTTCTTG UBC reverse primer:
(SEQ ID NO: 944) TGCCTTGACATTCTCGATGGT UBC-amplicon: (SEQ ID NO:
945) ATTTGGGTCGCGGTTCTTGTTTGTGGATCGCTGTGATCGTCACTTGACAATGCAGAT
CTTCGTGAAGACTCTGACTGGTAAGACCATCACCCTCGAGG
TTGAGCCCAGTGACACCATCGAGAATGTCAAGGCA SDHA (GenBank Accession No.
NM.sub.--004168 (SEQ ID NO: 922)) SDHA Forward primer: (SEQ ID NO:
923) TGGGAACAAGAGGGCATCTG SDHA reverse primer: (SEQ ID NO: 924)
CCACCACTGCATCAAATTCATG SDHA-amplicon: (SEQ ID NO: 925)
TGGGAACAAGAGGGCATCTGCTAAAGTTTCAGATTCCATTTCTGCTCAGTATCCAGT
AGTGGATCATGAATTTGATGCAGTGGTGG
[0661] Oligonucleotide-Based Micro-Array Experiment Protocol--
Microarray Fabrication
[0662] Microarrays (chips) were printed by pin deposition using the
MicroGrid II MGII 600 robot from BioRobotics Limited (Cambridge,
UK). 50-mer oligonucleotides target sequences were designed by
Compugen Ltd (Tel-Aviv, IL) as described by A. Shoshan et al,
"Optical technologies and informatics", Proceedings of SPIE. Vol
4266, pp. 86-95 (2001). The designed oligonucleotides were
synthesized and purified by desalting with the Sigma-Genosys system
(The Woodlands, Tex., US) and all of the oligonucleotides were
joined to a C6 amino-modified linker at the 5' end, or being
attached directly to CodeLink slides (Cat #25-6700-01. Amersham
Bioscience, Piscataway, N.J., US). The 50-mer oligonucleotides,
forming the target sequences, were first suspended in Ultra-pure
DDW (Cat # 01-866-1A Kibbutz Beit-Haemek, Israel) to a
concentration of 50.mu.M. Before printing the slides, the
oligonucleotides were resuspended in 300 mM sodium phosphate (pH
8.5) to final concentration of 150 mM and printed at 35-40%
relative humidity at 21.degree. C.
[0663] Each slide contained a total of 9792 features in 32
subarrays. Of these features, 4224 features were sequences of
interest according to the present invention and negative controls
that were printed in duplicate. An additional 288 features (96
target sequences printed in triplicate) contained housekeeping
genes from Human Evaluation Library2, Compugen Ltd, Israel. Another
384 features are E. coli spikes 1-6, which are oligos to E-Coli
genes which are commercially available in the Array Control product
(Array control-sense oligo spots, Ambion Inc. Austin, Tex. Cat #
1781, Lot # 112K06).
Post-Coupling Processing of Printed Slides
[0664] After the spotting of the oligonucleotides to the glass
(CodeLink) slides, the slides were incubated for 24 hours in a
sealed saturated NaCl humidification chamber (relative humidity
70-75%).
[0665] Slides were treated for blocking of the residual reactive
groups by incubating them in blocking solution at 50.degree. C for
15 minutes (10 ml/slide of buffer containing 0.1M Tris, 50 mM
ethanolamine, 0.1% SDS). The slides were then rinsed twice with
Ultra-pure DDW (double distilled water). The slides were then
washed with wash solution (10 ml/slide. 4.times.SSC, 0.1% SDS)) at
50.degree. C. for 30 minutes on the shaker. The slides were then
rinsed twice with Ultra-pure DDW, followed by drying by
centrifugation for 3 minutes at 800 rpm.
[0666] Next, in order to assist in automatic operation of the
hybridization protocol, the slides were treated with Ventana
Discovery hybridization station barcode adhesives. The printed
slides were loaded on a Bio-Optica (Milan, Italy) hematology
staining device and were incubated for 10 minutes in 50 ml of
3-Aminopropyl Triethoxysilane (Sigma A3648 lot #122K589). Excess
fluid was dried and slides were then incubated for three hours in
20 mm/Hg in a dark vacuum desiccator (Pelco 2251, Ted Pella, Inc.
Redding Calif.).
[0667] The following protocol was then followed with the Genisphere
900-RP (random primer), with mini elute columns on the Ventana
Discovery HybStation.TM., to perform the microarray experiments.
Briefly, the protocol was performed as described with regard to the
instructions and information provided with the device itself. The
protocol included cDNA synthesis and labeling. cDNA concentration
was measured with the TBS-380 (Turner Biosystems. Sunnyvale,
Calif.) PicoFlour, which is used with the OliGreen ssDNA
Quantitation reagent and kit. Hybridization was performed with the
Ventana Hybridization device, according to the provided protocols
(Discovery Hybridization Station Tuscon Ariz.).
[0668] The slides were then scanned with GenePix 4000B dual laser
scanner from Axon Instruments Inc, and analyzed by GenePix Pro 5.0
software.
[0669] Schematic summary of the oligonucleotide based microarray
fabrication and the experimental flow is presented in FIGS. 4 and
5.
[0670] Briefly, as shown in FIG. 4, DNA oligonucleotides at 25 uM
were deposited (printed) onto Amersham `CodeLink` glass slides
generating a well defined `spot`. These slides are covered with a
long-chain, hydrophilic polymer chemistry that creates an active
3-D surface that covalently binds the DNA oligonucleotides 5'-end
via the C6-amine modification. This binding ensures that the full
length of the DNA oligonucleotides is available for hybridization
to the cDNA and also allows lower background, high sensitivity and
reproducibility.
[0671] FIG. 5 shows a schematic method for performing the
microarray experiments. It should be noted that stages on the
left-hand or right-hand side may optionally be performed in any
order, including in parallel, until stage 4 (hybridization).
Briefly, on the left-hand side, the target oligonucleotides are
being spotted on a glass microscope slide (although optionally
other materials could be used) to form a spotted slide (stage 1).
On the right hand side, control sample RNA and cancer sample RNA
are Cy3 and Cy5 labeled, respectively (stage 2), to form labeled
probes. It should be noted that the control and cancer samples come
from corresponding tissues (for example, normal prostate tissue and
cancerous prostate tissue). Furthermore, the tissue from which the
RNA was taken is indicated below in the specific examples of data
for particular clusters, with regard to overexpression of an
oligonucleotide from a "chip" (microarray), as for example
"prostate" for chips in which prostate cancerous tissue and normal
tissue were tested as described above. In stage 3, the probes are
mixed. In stage 4, hybridization is performed to form a processed
slide. In stage 5, the slide is washed and scanned to form an image
file, followed by data analysis in stage 6.
Description for Cluster T10888
[0672] Cluster T10888 features 4 transcript(s) and 8 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00092 TABLE I Transcripts of interest Transcript Name
Sequence ID No. T10888_PEA.sub.--1_T11 T10888_PEA.sub.--1_T4 2
T10888_PEA.sub.--1_T5 3 T10888_PEA.sub.--1_T6 4
[0673] TABLE-US-00093 TABLE 2 Segments of interest Segment Name
Sequence ID No. T10888_PEA.sub.--1_node.sub.--11 5
T10888_PEA.sub.--1_node.sub.--12 6 T10888_PEA.sub.--1_node.sub.--17
7 T10888_PEA.sub.--1_node.sub.--4 8 T10888_PEA.sub.--1_node.sub.--6
9 T10888_PEA.sub.--1_node.sub.--7 10
T10888_PEA.sub.--1_node.sub.--9 11 T10888_PEA.sub.--1_node.sub.--15
12
[0674] TABLE-US-00094 TABLE 3 Proteins of interest Protein Name
Sequence ID No. T10888_PEA.sub.--1_P2 14 T10888_PEA.sub.--1_P4 15
T10888_PEA.sub.--1_P5 16 T10888_PEA.sub.--1_P6 17
[0675] These sequences are variants of the known protein
Carcinoembryonic antigen-related cell adhesion molecule 6 precursor
(SEQ ID NO:13) (SwissProt accession identifier CEA6_HUMAN; known
also according to the synonyms Normal cross-reacting antigen;
Nonspecific crossreacting antigen; CD66c antigen), SEQ ID NO:13,
referred to herein as the previously known protein.
[0676] The sequence for protein Carcinoembryonic antigen-related
cell adhesion molecule 6 precursor (SEQ ID NO:13) is given at the
end of the application, as "Carcinoembryonic antigen-related cell
adhesion molecule 6 precursor (SEQ ID NO:13) amino acid sequence".
Known polymorphisms for this sequence are as shown in Table 4.
TABLE-US-00095 TABLE 4 Amino acid mutations for Known Protein SNP
position(s) on amino acid sequence Comment 138 F->L 239
V->G
[0677] Protein Carcinoembryonic antigen-related cell adhesion
molecule 6 precursor (SEQ ID NO:13) localization is believed to be
Attached to the membrane by a GPI-anchor.
[0678] The previously known protein also has the following
indication(s) and/or potential therapeutic use(s): Cancer. It has
been investigated for clinical/therapeutic use in humans, for
example as a target for an antibody or small molecule, and/or as a
direct therapeutic; available information related to these
investigations is as follows. Potential pharmaceutically related or
therapeutically related activity or activities of the previously
known protein are as follows: Immunostimulant. A therapeutic role
for a protein represented by the cluster has been predicted. The
cluster was assigned this field because there was information in
the drug database or the public databases (e.g., described herein
above) that this protein, or part thereof, is used or can be used
for a potential therapeutic indication: Imaging agent; Anticancer;
Immunostimulant; Immunoconjugate; Monoclonal antibody, murine;
Antisense therapy; antibody.
[0679] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: signal
transduction; cell-cell signaling, which are annotation(s) related
to Biological Process; and integral plasma membrane protein, which
are annotation(s) related to Cellular Component.
[0680] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[0681] Cluster T10888 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the right hand column of the table and the numbers on the y-axis of
FIG. 6 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[0682] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 6 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: colorectal cancer, a mixture of malignant
tumors from different tissues, pancreas carcinoma and gastric
carcinoma. TABLE-US-00096 TABLE 5 Normal tissue distribution Name
of Tissue Number Bladder 0 Colon 107 Epithelial 52 General 22 head
and neck 40 Lung 237 Breast 0 pancreas 32 Prostate 12 Stomach 0
[0683] TABLE-US-00097 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4Bladder 5.4e-01
3.4e-01 5.6e-01 1.8 4.6e-01 1.9 Colon 1.2e-01 1.7e-01 2.8e-05 3.7
7.9e-04 2.8 epithelial 3.3e-02 2.1e-01 2.8e-20 2.8 4.8e-10 1.9
General 3.3e-05 2.2e-03 1.9e-44 4.9 4.6e-27 3.3 head and neck
4.6e-01 4.3e-01 1 0.8 7.5e-01 1.0 Lung 7.6e-01 8.2e-01 8.9e-01 0.6
1 0.3 Breast 3.7e-02 4.1e-02 1.5e-01 3.3 3.1e-01 2.4 pancreas
2.6e-01 2.4e-01 8.6e-23 2.8 1.5e-19 4.5 Prostate 9.1e-01 9.3e-01
4.1e-02 1.2 1.0e-01 1.0 Stomach 4.5e-02 5.6e-02 5.1e-04 4.1 4.7e-04
6.3
[0684] As noted above, cluster T10888 features 4 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Carcinoembryonic
antigen-related cell adhesion molecule 6 precursor (SEQ ID NO:13).
A description of each variant protein according to the present
invention is now provided.
[0685] Variant protein T10888_PEA.sub.--1_P2 (SEQ ID NO:14)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T10888_PEA.sub.--1_T1 (SEQ ID NO:1). An alignment is given to the
known protein (Carcinoembryonic antigen-related cell adhesion
molecule 6 precursor (SEQ ID NO:13)) at the end of the application.
One or more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0686] Comparison report between T10888_PEA.sub.--1_P2 (SEQ ID
NO:14) and CEA6_HUMAN (SEQ ID NO:13):
[0687] 1. An isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P2 (SEQ ID NO:14), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY
GPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS
YMCQAHNSATGLNRTTVTMITVS corresponding to amino acids 1-319 of
CEA6_HUMAN (SEQ ID NO:13), which also corresponds to amino acids
1-319 of T10888_PEA.sub.--1_P2 (SEQ ID NO:14), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence DWTRP (SEQ ID NO:999) corresponding to amino acids 320-324
of T10888_PEA.sub.--1_P2 (SEQ ID NO:14), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0688] 2. An isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P2 (SEQ ID NO:14), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence DWTRP (SEQ
ID NO:999) in T10888_PEA.sub.--1_P2 (SEQ ID NO:14).
[0689] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0690] Variant protein T10888_PEA.sub.--1_P2 (SEQ ID NO:14) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T10888_PEA.sub.--1_P2 (SEQ ID
NO:14) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00098
TABLE 7 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 13
V->No 232 N->D No 324 P->No 63 I->No 92 G->No
[0691] Variant protein T10888_PEA.sub.--1_P2 (SEQ ID NO:14) is
encoded by the following transcript(s): T10888_PEA.sub.--1_T1 (SEQ
ID NO:1), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript T10888_PEA.sub.--1_T1
(SEQ ID NO:1) is shown in bold; this coding portion starts at
position 151 and ends at position 1122. The transcript also has the
following SNPs as listed in Table 8 (given according to their
position on the nucleotide sequence, with the alternative nucleic
acid listed; the last column indicates whether the SNP is known or
not; the presence of known SNPs in variant protein
T10888_PEA.sub.--1.sub.--P2 (SEQ ID NO:14) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00099 TABLE 8 Nucleic acid SNPs
SNP position on Alternative Previously nucleotide sequence nucleic
acid known SNP? 119 C->T No 120A->T No 1062A->G Yes 1120
C->No 1297G->T Yes 1501 A->G Yes 1824 G->A No 2036
A->C No 2036 A->G No 2095 A->C No 2242 A->C No 2245
A->C No 189 C->No 2250 A->T Yes 2339 C->A Yes 276
G->A Yes 338 T->No 424 G->No 546 A->G No 702 C->T No
844 A->G No 930 C->T Yes
[0692] Variant protein T10888_PEA.sub.--1_P4 (SEQ ID NO:15
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T10888_PEA.sub.--1_T4 (SEQ ID NO:2). An alignment is given to the
known protein (Carcinoembryonic antigen-related cell adhesion
molecule 6 precursor (SEQ ID NO:13)) at the end of the application.
One or more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0693] Comparison report between T10888_PEA.sub.--1_P4 (SEQ ID
NO:15) and CEA6_HUMAN SEQ ID NO:13):
[0694] 1. An isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a first amino acid
sequence being at least 90% homologous to TABLE-US-00100
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL
corresponding to amino acids 1-234 of CEA6_HUMAN (SEQ ID NO:13),
which also corresponds to amino acids 1-234 of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) corresponding to amino
acids 235-256 of T10888_PEA.sub.--1_P4 (SEQ ID NO:15), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0695] 2. An isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) in T10888_PEA.sub.--1_P4
(SEQ ID NO:15).
[0696] Comparison report between T10888_PEA.sub.--1_P4 (SEQ ID
NO:15) and Q13774 (SEQ ID NO:829):
[0697] 1. An isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL
corresponding to amino acids 1-234 of Q13774, which also
corresponds to amino acids 1-234 of T10888_PEA.sub.--1_P4 (SEQ ID
NO:15), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence LLLSSQLWPPSASRLECWPGWL (SEQ ID
NO:1000) corresponding to amino acids 235-256 of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0698] 2. An isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P4 (SEQ ID NO:15), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
LLLSSQLWPPSASRLECWPGWL (SEQ ID NO:1000) in T10888_PEA.sub.--1_P4
(SEQ ID NO:15).
[0699] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0700] Variant protein T10888_PEA.sub.--1_P4 (SEQ ID NO:15 also has
the following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 9, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T10888_PEA.sub.--1_P4 (SEQ ID
NO:15) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00101
TABLE 9 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 13
V->No 232 N->D No 63 I->No 92 G->No
[0701] Variant protein T10888_PEA.sub.--1_P4 (SEQ ID NO:15) is
encoded by the following transcript(s): T10888_PEA.sub.--1_T4 (SEQ
ID NO:2), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript T10888_PEA.sub.--1_T4
(SEQ ID NO:2) is shown in bold; this coding portion starts at
position 151 and ends at position 918. The transcript also the
following SNPs as listed in Table 10 (given according to their
position on the nucleotide sequence, with the alternative nucleic
acid listed; the last column indicates whether the SNP is known or
not; the presence of known SNPs in variant protein
T10888_PEA.sub.--1_P4 SEQ ID NO: 15) sequence provides support for
the deduced sequence of this variant protein according to the
present invention). TABLE-US-00102 TABLE 10 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 119 C->T No 120A->T No 978 C->No 1155 G->T
Yes 1359A->G Yes 1682 G->A No 1894A->C No 1894 A->G No
1953 A->C No 2100 A->C No 2103 A->C No 2108 A->T Yes
189 C->No 2197 C->A Yes 276 G->A Yes 338 T->No 424
G->No 546 A->G No 702 C->T No 844 A->G No 958
G->No
[0702] Variant protein T10888_PEA.sub.--1_P5 (SEQ ID NO:16)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T10888_PEA.sub.--1_T5 (SEQ ID NO:3). An alignment is given to the
known protein (Carcinoembryonic antigen-related cell adhesion
molecule 6 precursor (SEQ ID NO:13)) at the end of the application.
One or more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0703] Comparison report between T10888_PEA.sub.--1_P5 (SEQ ID
NO:16) and CEA6_HUMAN (SEQ ID NO:13):
[0704] 1. An isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), comprising a first amino acid
sequence being at least 90% homologous to
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLP
QNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTG
FYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYL
WWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLY
GPDVPTISPSKANYRPGENLNLSCHMSNPPAQYSWFINGTFQQSTQELFIPNITVNNSGS
YMCQAHNSATGLNRTTVTMITVSG corresponding to amino acids 1-320 of
CEA6_HUMAN (SEQ ID NO:13), which also corresponds to amino acids
1-320 of T10888_PEA.sub.--1_P5 (SEQ ID NO:16), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence
KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF
VVFCFLISHV (SEQ ID NO:1001) corresponding to amino acids 321-390 of
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0705] 2. An isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P5 (SEQ ID NO:16), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00103
KWIHEALASHFQVESGSQRRARKKFSFPTCVQGAHANPKFSPEPSQFTSADSFPLVFLFF (SEQ
ID NO: 1001) VVFCFLISHV in T10888_PEA.sub.--1_P5. (SEQ ID NO:
16)
[0706] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: membrane. The protein localization is
believed to be membrane because although both signal-peptide
prediction programs agree that this protein has a signal peptide,
both trans-membrane region prediction programs predict that this
protein has a trans-membrane region downstream of this signal
peptide.
[0707] Variant protein T10888_PEA.sub.--1_P5 (SEQ ID NO:16) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T10888_PEA.sub.--1_P5 (SEQ ID
NO:16) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00104
TABLE 11 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 13
V->No 232 N->D No 63 I->No 92 G->No
[0708] Variant protein T10888_PEA.sub.--1_P5 (SEQ ID NO 16) is
encoded by the following transcript(s): T10888_PEA.sub.--1_T5 (SEQ
ID NO:3), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript T10888_PEA.sub.--1_T5
(SEQ ID NO:3) is shown in bold; this coding portion starts at
position 151 and ends at position 1320. The transcript also has the
following SNPs as listed in Table 12 (given according to their
position on the nucleotide sequence, with the alternative nucleic
acid listed; the last column indicates whether the SNP is known or
not; the presence of known SNPs in variant protein
T10888_PEA.sub.--1_P5 (SEQ ID NO:16) sequence provides support for
the deduced sequence of variant protein according to the present
invention). TABLE-US-00105 TABLE 12 Nucleic acid SNPs SNP position
on Alternative Previously nucleotide sequence nucleic acid known
SNP? 119 C->T No 120 A->T No 1062 A->G Yes 1943 C->A
Yes 2609 C->T Yes 2647 C->G No 2701 C->T Yes 2841 T->C
Yes 189 C->No 276 G->A Yes 338 T->No 424 G->No 546
A->G No 702 C->T No 844 A->G No 930 C->T Yes
[0709] Variant protein T10888_PEA.sub.--1_P6 (SEQ ID NO:17)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T10888_PEA.sub.--1_T6 (SEQ ID NO:4). An alignment is given to the
known protein (Carcinoembryonic antigen-related cell adhesion
molecule 6 precursor (SEQ ID NO:13)) at the end of the application.
One or more alignments to one or more previously published protein
sequences are given at the end of the application.
[0710] Comparison report between T10888_PEA.sub.--1_P6 (SEQ ID
NO:17) and CEA6_HUMAN (SEQ ID NO:13):
[0711] 1. An isolated chimeric polypeptide encoding for
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), comprising a first amino acid
sequence being at least 90% homologous to TABLE-US-00106
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLA
HNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQ
NDTGFYTLQVIKSDLVNEEATGQFHVY
[0712] corresponding to amino acids 1-141 of CEA6_HUMAN (SEQ ID
NO:13), which also corresponds to amino acids 1-141 of
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TABLE-US-00107 (SEQ ID NO: 1002)
REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI
[0713] corresponding to amino acids 142-183 of
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0714] 2. An isolated polypeptide encoding for a tail of
T10888_PEA.sub.--1_P6 (SEQ ID NO:17), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00108 (SEQ ID NO: 1002)
REYFHMTSGCWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI (SEQ ID NO: 17) in
T10888_PEA.sub.--1_P6.
[0715] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0716] Variant protein T10888_PEA.sub.--1_P6 (SEQ ID NO:17) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T10888_PEA.sub.--1_P6 (SEQ ID
NO:17) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00109
TABLE 13 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 13
V->No 63 I->No 92 G->No
[0717] Variant protein T10888_PEA.sub.--1_P6 (SEQ ID NO:17) is
encoded by the following transcript(s): T10888_PEA.sub.--1_T6 (SEQ
ID NO:4), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript T10888_PEA.sub.--1_T6
(SEQ ID NO:4) is shown in bold; this coding portion starts at
position 151 and ends at position 699. The transcript also as the
following SNPs as listed in Table 14 (given according to their
position on the nucleotide sequence, with the alternative nucleic
acid listed; the last column indicates whether the SNP is known or
not; the presence of known SNPs in variant protein
T10888_PEA.sub.--1_P6 (SEQ ID NO:17) sequence provides support for
the deduced sequence of this variant protein according to the
present invention). TABLE-US-00110 TABLE 14 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 119 C->T No 120 A->T No 189 C->No 276 G->A
Yes 338 T->No 424 G->No 546 A->G No
[0718] As noted above, cluster T10888 features 8 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[0719] Segment cluster T10888_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:5) according to the present invention is supported by 57
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T1 (SEQ ID NO:1) and
T10888_PEA.sub.--1_T5 (SEQ ID NO:3). Table 15 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00111 TABLE 15 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T1 (SEQ ID 854 1108 NO: 1) T10888_PEA.sub.--1_T5
(SEQ ID 854 1108 NO: 3)
[0720] Segment cluster T10888_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:6) according to the present invention is supported by 9
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T5 (SEQ ID NO:3). Table 16 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00112 TABLE 16 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T5 (SEQ ID 1109 3004 NO: 3)
[0721] Segment cluster T10888_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:7) according to the present invention is supported by 160
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T1 (SEQ ID NO:1) and
T10888_PEA.sub.--1_T4 (SEQ ID NO:2). Table 17 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00113 TABLE 17 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T1 (SEQ ID 1109 2518 NO: 1)
T10888_PEA.sub.--1_T4 (SEQ ID 967 2376 NO: 2)
[0722] Segment cluster T10888_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:8) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T1 (SEQ ID NO:1),
T10888_PEA.sub.--1_T4 (SEQ ID NO:2), T10888_PEA.sub.--1_T5 (SEQ ID
NO:3) and T10888_PEA.sub.--1_T6 (SEQ ID NO:4). Table 18 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00114 TABLE 18 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T1 (SEQ ID 1 214 NO: 1) T10888_PEA.sub.--1_T4
(SEQ ID 1 214 NO: 2) T10888_PEA.sub.--1_T5 (SEQ ID 1 214 NO: 3)
T10888_PEA.sub.--1_T6 (SEQ ID 1 214 NO: 4)
[0723] Segment cluster T10888_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:9) according to the present invention is supported by 81
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T1 (SEQ ID NO:1),
T10888_PEA.sub.--1_T4 (SEQ ID NO:2), T10888_PEA.sub.--1_T5 (SEQ ID
NO:3) and T10888_PEA.sub.--1_T6 (SEQ ID NO:4). Table 19 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00115 TABLE 19 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T1 (SEQ ID 215 574 NO: 1) T10888_PEA.sub.--1_T4
(SEQ ID 215 574 NO: 2) T10888_PEA.sub.--1_T5 (SEQ ID 215 574 NO: 3)
T10888_PEA.sub.--1_T6 (SEQ ID 215 574 NO: 4)
[0724] Segment cluster T10888_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:10) according to the present invention is supported by 4
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T6 (SEQ ID NO:4). Table 20 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00116 TABLE 20 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T10888_PEA.sub.--1_T6 (SEQ ID 575 1410 NO: 4)
[0725] Segment cluster T10888_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:11) according to the present invention is supported by 72
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T1 (SEQ ID NO:1),
T10888_PEA.sub.--1_T4 (SEQ ID NO:2) and T10888_PEA.sub.--1_T5 (SEQ
ID NO:3). Table 21 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00117 TABLE 21 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T10888_PEA.sub.--1_T1 (SEQ ID 575 853 NO:
1) T10888_PEA.sub.--1_T4 (SEQ ID 575 853 NO: 2)
T10888_PEA.sub.--1_T5 (SEQ ID 575 853 NO: 3)
[0726] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[0727] Segment cluster T10888_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:12) according to the present invention is supported by 39
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T10888_PEA.sub.--1_T4 (SEQ ID NO:2). Table 22 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-001 18 TABLE 22 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T10888_PEA.sub.--1_T4 (SEQ ID 854 966 NO: 2)
[0728] Variant protein alignment to the previously known protein:
Sequence name: /tmp/tM4EgaoKvm/vuztUrlRc7:CEA6_HUMAN (SEQ ID
NO:13).
[0729] Sequence Documentation:
[0730] Alignment of: T10888_PEA.sub.--1_P2 (SEQ ID
NO:14).times.CEA6_HUMAN (SEQ ID NO:13)
[0731] Alignment segment 1/1: TABLE-US-00119 Quality: 3163.00
Escore: 0 Matching length: 319 Total length: 319 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00001 Alignment: TABLE-US-00120 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150 . . . 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200 . . . 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDVPTISPSKANYR 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDVPTISPSKANYR 250 . . . 251
PGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQ 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
PGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQ 300 . . . 301
AHNSATGLNRTTVTMITVS 319 ||||||||||||||||||| 301 AHNSATGLNRTTVTMITVS
319
[0732] Sequence name: /tmp/YjI1gj7TCe/PgdufzLOIW:CEA6_HUMAN (SEQ ID
NO:13)
[0733] Sequence Documentation:
[0734] Alignment of: T10888_PEA.sub.--1_P4 (SEQ ID
NO:15).times.CEA6_HUMAN (SEQ ID NO: 13).
[0735] Alignment segment 1/1: TABLE-US-00121 Quality: 2310.00
Escore: 0 Matching length: 234 Total length: 234 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00002 Alignment: TABLE-US-00122 . . . 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATCQFHVYPELPKPSIS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150 . . . 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200 . . . 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL 234
|||||||||||||||||||||||||||||||||| 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL 234
[0736] Sequence name: /tmp/YjI1gj7TCe/PgdufzLOIW:Q13774
[0737] Sequence Documentation:
[0738] Alignment of: T10888_PEA.sub.--1_P4 (SEQ ID
NO:15).times.Q13774.
[0739] Alignment segment 1/1: TABLE-US-00123 Quality: 2310.00
Escore: 0 Matching length: 234 Total length: 234 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00003 Alignment: TABLE-US-00124 . . . 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150 . . . 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200 . . . 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL 234
|||||||||||||||||||||||||||||||||| 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVL 234
[0740] Sequence name: /tmp/x5xDBacdpj/rTXRGepv3y:CEA6_HUMAN (SEQ ID
NO:13)
[0741] Sequence Documentation:
[0742] Alignment of: T10888_PEA.sub.--1_P5 (SEQ ID
NO:16).times.CEA6_HUMAN (SEQ ID NO: 13).
[0743] Alignment segment 1/1: TABLE-US-00125 Quality: 3172.00
Escore: 0 Matching length: 320 Total length: 320 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00004 Alignment: TABLE-US-00126 . . . 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSIS 150 . . . 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
SNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTL 200 . . . 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDVPTISPSKANYR 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
TLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDVPTISPSKANYR 250 . . . 251
PGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQ 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
PGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQ 300 . . . 301
AHNSATGLNRTTVTMITVSG 320 |||||||||||||||||||| 301
AHNSATGLNRTTVTMITVSG 320
[0744] Sequence name: /tmp/VAhvYFeatq/QNEM573uCo:CEA6_HUMAN (SEQ ID
NO:13)
[0745] Sequence Documentation:
[0746] Alignment of: T10888_PEA.sub.--1_P6 (SEQ ID
NO:17).times.CEA6_HUMAN (SEQ ID NO: 13).
[0747] Alignment segment 1/1: TABLE-US-00127 Quality: 1393.00
Escore: 0 Matching length: 143 Total length: 143 Matching Percent
99.30 Matching Percent Identity: 99.30 Similarity: Total Percent
Similarity: 99.30 Total Percent Identity: 99.30 Gaps: 0
TABLE-US-00005 Alignment: TABLE-US-00128 . . . 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYRE 143
||||||||||||||||||||||||||||||||||||||||| |101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPE 143
[0748] Alignment of: T10888_PEA.sub.--1_P6 (SEQ ID
NO:17).times.CEA6_HUMAN (SEQ ID NO: 13).
[0749] Alignment segment 1/1: TABLE-US-00129 Quality: 101.00
Escore: 0 Matching length: 141 Total length: 183 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 77.05 Total Percent Identity: 77.05 Gaps: 1
TABLE-US-00006 Alignment: TABLE-US-00130 . . . 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKE 50 . . . 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRET 100 . . . 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYREYFHMTSG 150
||||||||||||||||||||||||||||||||||||||||| 101
IYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVY.........141 . . . 151
CWGSVLLPTYGIVRPGLCLWPSLHYILYQGLDI 183 141 .......................
141
Expression of CEA6_HUMAN Carcinoembryonic Antigen-Related Cell
Adhesion Molecule 6 (T10888) Transcripts Which are Detectable by
Amplicon as Depicted in Sequence Name T10888 junc11-17 in Normal
and Cancerous Breast Tissues
[0750] Expression of CEA6_HUMAN Carcinoembryonic antigen-related
cell adhesion molecule 6 transcripts detectable by or according to
junc11-17, T10888junc11-17 (SEQ ID NO:832) amplicon(s) and
T10888junc1-17F (SEQ ID NO:830) and T10888junc11-17R primers was
measured by real time PCR. In parallel the expression of four
housekeeping genes--PBGD (GenBank Accession No. BC019323 (SEQ ID
NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank
Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922Q;
amplicon--SDHA-amplicon (SEQ ID NO:925)), G6PD (GenBank Accession
No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ ID NO:921))
was measured similarly. For each RT sample, the expression of the
above amplicon was normalized to the geometric mean of the
quantities of the housekeeping genes. The normalized quantity of
each RT sample was then divided by the median of the quantities of
the normal post-mortem (PM) samples (Sample Nos. 56-60, 63-67 Table
1, "Tissue samples in testing panel", above), to obtain a value of
fold up-regulation for each sample relative to median of the normal
PM samples.
[0751] FIG. 7 is a histogram showing over expression of the
above-indicated CEA6_HUMAN Carcinoembryonic antigen-related cell
adhesion molecule 6 transcripts in cancerous breast samples
relative to the normal samples. Values represent the average of
duplicate experiments. Error bars indicate the minimal and maximal
values obtained. The number and percentage of samples that exhibit
at least 5 fold over-expression, out of the total number of samples
tested, is indicated in the bottom.
[0752] As is evident from FIG. 7, the expression of CEA6_HUMAN
Carcinoembryonic antigen-related cell adhesion molecule 6
transcripts detectable by the above amplicon(s) in cancer samples
was significantly higher than in the non-cancerous samples (Sample
Nos. 56-60, 63-67 Table 1, "Tissue samples in testing panel").
Notably an over-expression of at least 5 fold was found in 19 out
of 28 adenocarcinoma samples.
[0753] Statistical analysis was applied to verify the significance
of these results, as described below.
[0754] The P value for the difference in the expression levels of
CEA6_HUMAN Carcinoembryonic antigen-related cell adhesion molecule
6 transcripts detectable by the above amplicon(s) in breast cancer
samples versus the normal tissue samples was determined by T test
as 2.00E-03.
[0755] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
8.44E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[0756] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair:
T10888junc11-17F (SEQ ID NO:830) forward primer; and
T10888junc11-17R reverse primer.
[0757] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: T10888junc11-17. TABLE-US-00131 T10888junc11-17F (SEQ ID
NO: 830) CCAGCAATCCACACAAGAGCT T10888junc11-17R (SEQ ID NO: 831)
CAGGGTCTGGTCCAATCAGAG T10888junc11-17 (SEQ ID NO: 832)
CCAGCAATCCACACAAGAGCTCTTTATCCCCAACATCACTGTGAATAATAGC
GGATCCTATATGTGCCAAGCCCATAACTCAGCCACTGGCCTCAATAGGACCACAGT
CACGATGATCACAGTCTCTGATTGGACCAGACCCTG
Expression of CEA6_HUMAN Carcinoembryonic Antigen-Related Cell
Adhesion Molecule 6T10888 Transcripts Which are Detectable by
Amplicon as Depicted in Sequence Name T10888junc1-17 (SEQ ID
NO:832) in Different Normal Tissues
[0758] Expression of CEA6_HUMAN Carcinoembryonic antigen-related
cell adhesion molecule 6 transcripts detectable by or according to
T10888 junc11-17 amplicon(s) (SEQ ID NO:832) and T10888 junc11-17F
(SEQ ID NO:830) and T10888 junc11-17R (SEQ ID NO:831) was measured
by real time PCR. In parallel the expression of four housekeeping
genes--RPL19 (GenBank Accession No. NM.sub.--000981 (SEQ ID
NO:934); RPL19 amplicon (SEQ ID NO:937)), TATA box (GenBank
Accession No. NM.sub.--003194 (SEQ ID NO:938); TATA amplicon (SEQ
ID NO:941)), UBC (GenBank Accession No. BC000449 (SEQ ID NO:942);
amplicon--Ubiquitin-amplicon (SEQ ID NO:945) and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the ovary samples
(Sample Nos. 18-20, Table 2 "Tissue samples in normal panel"
above), to obtain a value of relative expression of each sample
relative to median of the ovary samples. Primers and amplicon are
as above.
[0759] The results are presented in FIG. 8, demonstrating the
expression of CEA6_HUMAN Carcinoembryonic antigen-related cell
adhesion molecule 6 T10888 transcripts, which are detectable by
amplicon as depicted in sequence name T10888junc11-17 (SEQ ID
NO:832), in different normal tissues.
Description for Cluster T39971
[0760] Cluster T39971 features 4 transcript(s) and 28 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-001 32 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. T39971_T10 18 T39971_T12 19 T39971_T16 20 T39971_T5
21
[0761] TABLE-US-00133 TABLE 2 Segments of interest Segment Name
Sequence ID No. T39971_node.sub.--0 22 T39971_node.sub.--18 23
T39971_node.sub.--21 24 T39971_node.sub.--22 25
T39971_node.sub.--23 26 T39971_node.sub.--31 27
T39971_node.sub.--33 28 T39971_node.sub.--7 29 T39971_node.sub.--1
30 T39971_node.sub.--10 31 T39971_node.sub.--11 32
T39971_node.sub.--12 33 T39971_node.sub.--15 34
T39971_node.sub.--16 35 T39971_node.sub.--17 36
T39971_node.sub.--26 37 T39971_node.sub.--27 38
T39971_node.sub.--28 39 T39971_node.sub.--29 40 T39971_node.sub.--3
41 T39971_node.sub.--30 42 T39971_node.sub.--34 43
T39971_node.sub.--35 44 T39971_node.sub.--36 45 T39971_node.sub.--4
46 T39971_node.sub.--5 47 T39971_node.sub.--8 48
T39971_node.sub.--9 49
[0762] TABLE-US-00134 TABLE 3 Proteins of interest Protein Name
Sequence ID No. T39971_P6 51 T39971_P9 52 T39971_P11 53 T39971_P12
54
[0763] These sequences are variants of the known protein
Vitronectin precursor (SwissProt accession identifier VTNC_HUMAN;
known also according to the synonyms Serum spreading factor;
S-protein; V75), SEQ ID NO:50, referred to herein as the previously
known protein.
[0764] Protein Vitronectin precursor (SEQ ID NO:50) is known or
believed to have the following function(s): Vitronectin is a cell
adhesion and spreading factor found in serum and tissues.
Vitronectin interacts with glycosaminoglycans and proteoglycans. Is
recognized by certain members of the integrin family and serves as
a cell-to-substrate adhesion molecule. Inhibitor of the
membrane-damaging effect of the terminal cytolytic complement
pathway. The sequence for protein Vitronectin precursor is given at
the end of the application, as "Vitronectin precursor amino acid
sequence" (SEQ ID NO:50). Known polymorphisms for this sequence are
as shown in Table 4. TABLE-US-001 35 TABLE 4 Amino acid mutations
for Known Protein SNP position(s) on amino acid sequence Comment
122 A->S./FTId=VAR.sub.--012983. 268
R->Q./FTId=VAR.sub.--012984. 400 T->M./FTId=VAR.sub.--012985.
50 C->N 225 S->N 366 A->T
[0765] Protein Vitronectin precursor (SEQ ID NO:50) localization is
believed to be Extracellular.
[0766] The previously known protein also has the following
indication(s) and/or potential therapeutic use(s): Cancer,
melanoma. It has been investigated for clinical/therapeutic use in
humans, for example as a target for an antibody or small molecule,
and/or as a direct therapeutic; available information related to
these investigations is as follows. Potential pharmaceutically
related or therapeutically related activity or activities of the
previously known protein are as follows: Alphavbeta3 integrin
antagonist; Apoptosis agonist. A therapeutic role for a protein
represented by the cluster has been predicted. The cluster was
assigned this field because there was information in the drug
database or the public databases (e.g., described herein above)
that this protein, or part thereof, is used or can be used for a
potential therapeutic indication: Anticancer.
[0767] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: immune response;
cell adhesion, which are annotation(s) related to Biological
Process; protein binding; heparin binding, which are annotation(s)
related to Molecular Function; and extracellular space, which are
annotation(s) related to Cellular Component.
[0768] The GO assignment relies on information from one or more of
the SwissPro/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[0769] Cluster T39971 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the right hand column of the table and the numbers on the y-axis of
FIG. 9 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[0770] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 9 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: liver cancer, lung malignant tumors and
pancreas carcinoma. TABLE-US-00136 TABLE 5 Normal tissue
distribution Name of Tissue Number adrenal 60 bladder 0 Bone 0
Brain 9 Colon 0 epithelial 79 general 29 Liver 2164 Lung 0 lymph
nodes 0 breast 0 pancreas 0 prostate 0 Skin 0 uterus 0
[0771] TABLE-US-00137 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 6.9e-01
7.4e-01 2.0e-02 2.3 5.3e-02 1.8 bladder 5.4e-01 6.0e-01 5.6e-01 1.8
6.8e-01 1.5 Bone 1 6.7e-01 1 1.0 7.0e-01 1.4 Brain 8.0e-01 8.6e-01
3.0e-01 1.9 5.3e-01 1.2 Colon 4.2e-01 4.8e-01 7.0e-01 1.6 7.7e-01
1.4 epithelial 6.6e-01 5.7e-01 1.0e-01 0.8 8.7e-01 0.6 general
5.1e-01 3.8e-01 9.2e-08 1.6 8.3e-04 1.3 Liver 1 6.7e-01 2.3e-03 0.3
1 0.2 Lung 2.4e-01 9.1e-02 1.7e-01 4.3 8.1e-03 5.0 lymph nodes 1
5.7e-01 1 1.0 5.8e-01 2.3 breast 1 6.7e-01 1 1.0 8.2e-01 1.2
pancreas 9.5e-02 1.8e-01 1.5e-11 6.5 8.2e-09 4.6 prostate 7.3e-01
6.0e-01 6.7e-01 1.5 5.6e-01 1.7 Skin 1 4.4e-01 1 1.0 6.4e-01 1.6
uterus 5.0e-01 2.6e-01 1 1.1 8.0e-01 1.4
[0772] As noted above, cluster T39971 features 4 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Vitronectin precursor
(SEQ ID NO:50). A description of each variant protein according to
the present invention is now provided.
[0773] Variant protein T39971_P6 (SEQ ID NO:51) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T39971_T5 (SEQ ID
NO:21). An alignment is given to the known protein (Vitronectin
precursor (SEQ ID NO:50)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0774] Comparison report between T39971_P6 (SEQ ID NO:51) and
VTNC_HUMAN (SEQ ID NO:50):
[0775] 1. An isolated chimeric polypeptide encoding for T39971_P6
(SEQ ID NO:51), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDMLALPAHSYSGRERVYFFKG corresponding to amino
acids 1-276 of VTNC_HUMAN (SEQ ID NO:50), which also corresponds to
amino acids 1-276 of T39971_P6 (SEQ ID NO:51), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence TQGVVGD (SEQ ID NO:1003) corresponding to amino acids
277-283 of T39971_P6 (SEQ ID NO:51), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0776] 2. An isolated polypeptide encoding for a tail of T39971_P6
(SEQ ID NO:51), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence TQGVVGD (SEQ ID NO:1003) in
T39971_P6 (SEQ ID NO:51).
[0777] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0778] Variant protein T39971_P6 (SEQ ID NO:51) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T39971_P6 (SEQ ID NO:51) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-001 38 TABLE 7 Amino
acid mutations SNP position(s) on amino acid Alternative sequence
amino acid(s) Previously known SNP? 122 A->S Yes 145 G->No
268 R->Q Yes 280 V->A Yes 180 C->No 180 C->W No 192
Y->No 209 A->No 211 T->No 267 G->No 267 G->A No 268
R->No
[0779] Variant protein T39971_P6 (SEQ ID NO:51) is encoded by the
following transcript(s): T39971_T5 (SEQ ID NO:21), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T39971_T5 (SEQ ID NO:21) is shown in bold;
this coding portion starts at position 756 and ends at position
1604. The transcript also has the following SNPs as listed in Table
8 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T39971_P6 (SEQ ID NO:51) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00139 TABLE 8 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 417 G->C Yes 459 T->C Yes 1387 C->No 1406->A
No 1406->G No 1555 G->No 1555 G->C No 1558 G->No 1558
G->A Yes 1594 T->C Yes 1642 T->C Yes 1770 C->T Yes 529
G->T Yes 1982 A->G No 2007 G->No 2029 T->C No 2094
T->C No 2117 C->G No 2123 C->T Yes 2152 C->T Yes 2182 G
->T No 2185 A->C No 2297 T->C Yes 1119 G->T Yes 2411
G->No 2411 G->T No 2487 T->C Yes 1188 G->No 1295
C->No 1295 C->G No 1324->T No 1331 C->No 1381
C->No
[0780] Variant protein T39971_P9 (SEQ ID NO:52) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T39971_T10 (SEQ ID
NO:18). An alignment is given to the known protein (Vitronectin
precursor (SEQ ID NO:50)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0781] Comparison report between T39971_P9 (SEQ ID NO:52) and
VTNC_HUMAN (SEQ ID NO:50):
[0782] 1. An isolated chimeric polypeptide encoding for T39971_P9
(SEQ ID NO:52), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRT corresponding to amino acids
1-325 of VTNC_HUMAN (SEQ ID NO:50), which also corresponds to amino
acids 1-325 of T39971_P9 (SEQ ID NO:52), and a second amino acid
sequence being at least 90% homologous to
SGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNONSRRPSRATWLSLFSSEESNLGA
NNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGC PAPGHL
corresponding to amino acids 357-478 of VTNC_HUMAN (SEQ ID NO:50),
which also corresponds to amino acids 326-447 of T39971_P9 (SEQ ID
NO:52), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0783] 2. An isolated chimeric polypeptide encoding for an edge
portion of T39971_P9 (SEQ ID NO:52), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise TS, having a structure as follows: a sequence
starting from any of amino acid numbers 325-x to 325; and ending at
any of amino acid numbers 326+((n-2)-x), in which x varies from 0
to n-2.
[0784] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0785] Variant protein T39971_P9 (SEQ ID NO:52) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 9, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T39971_P9 (SEQ ID NO:52) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00140 TABLE 9 Amino
acid mutations SNP position(s) on amino acid Alternative sequence
amino acid(s) Previously known SNP? 122 A->S Yes 145 G->No
268 R->Q Yes 328 M->T No 350 S->P No 369 T->M Yes 379
S->I No 380 N->T No 180 C->No 180 C->W No 192 Y->No
209A->No 211 T->No 267 G->No 267 G->A No 268
R->No
[0786] Variant protein T39971_P9 (SEQ ID NO:52) is encoded by the
following transcript(s): T39971_T10 (SEQ ID NO:18), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T39971_T10 (SEQ ID NO:18) is shown in bold;
this coding portion starts at position 756 and ends at position
2096. The transcript also has the following SNPs as listed in Table
10 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T39971_P9 (SEQ ID NO:52) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00141 TABLE 10 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 417 G->C Yes 459 T->C Yes 1387 C->No 1406->A
No 1406->G No 1555 G->No 1555 G->C No 1558 G->No 1558
G->A Yes 1738 T->C No 1803 T->C No 1826 C->G No 529
G->T Yes 1832 C->T Yes 1861 C->T Yes 1891 G->T No 1894
A->C No 2006 T->C Yes 2120 G->No 2120 G->T No 2196
T->C Yes 1119 G->T Yes 1188 G->No 1295 C->No 1295
C->G No 1324->T No 1331 C->No 1381 C->No
[0787] Variant protein T39971_P11 (SEQ ID NO:53) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T39971_T12 (SEQ ID
NO:19). An alignment is given to the known protein (Vitronectin
precursor (SEQ ID NO:50)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0788] Comparison report between T39971_P11 (SEQ ID NO:53) and
VTNC_HUMAN (SEQ ID NO:50):
[0789] 1. An isolated chimeric polypeptide encoding for T39971_P11
(SEQ ID NO:53), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDMFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDMLALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids
1-326 of VTNC_HUMAN (SEQ ID NO:50), which also corresponds to amino
acids 1-326 of T39971_P11 (SEQ ID NO:53), and a second amino acid
sequence being at least 90% homologous to
DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL corresponding to amino acids
442-478 of VTNC_HUMAN (SEQ ID NO:50), which also corresponds to
amino acids 327-363 of T39971_P11 (SEQ ID NO:53), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0790] 2. An isolated chimeric polypeptide encoding for an edge
portion of T39971_P11 (SEQ ID NO:53), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise SD, having a structure as follows: a sequence
starting from any of amino acid numbers 326-x to 326; and ending at
any of amino acid numbers 327+((n-2)-x), in which x varies from 0
to n-2.
[0791] Comparison report between T39971_P11 (SEQ ID NO:53) and
Q9BSH7 (SEQ ID NO:833):
[0792] 1. An isolated chimeric polypeptide encoding for T39971_P11
(SEQ ID NO:53), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDDMFTRINCQGKTYLFKGSQYWRFEDGV
LDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEE
CEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTS corresponding to amino acids
1-326 of Q9BSH7, which also corresponds to amino acids 1-326 of
T39971_P11 (SEQ ID NO:53), and a second amino acid sequence being
at least 90% homologous to DKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
corresponding to amino acids 442-478 of Q9BSH7, which also
corresponds to amino acids 327-363 of T39971_P11 (SEQ ID NO:53),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0793] 2. An isolated chimeric polypeptide encoding for an edge
portion of T39971_P11 (SEQ ID NO:53), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise SD, having a structure as follows: a sequence
starting from any of amino acid numbers 326-x to 326; and ending at
any of amino acid numbers 327+((n-2)-x), in which x varies from 0
to n-2.
[0794] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0795] Variant protein T39971_P11 (SEQ ID NO:53) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T39971_P11 (SEQ ID NO:53) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00142 TABLE 11 Amino
acid mutations SNP position(s) on amino acid Alternative sequence
amino acid(s) Previously known SNP? 122 A->S Yes 145 G->No
268 R->Q Yes 180 C->No 180 C->W No 192 Y->No 209
A->No 211 T->No 267 G->No 267 G->A No 268 R->No
[0796] Variant protein T39971_P11 (SEQ ID NO:53) is encoded by the
following transcript(s): T39971_T12 (SEQ ID NO:19), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T39971_T12 (SEQ ID NO:19) is shown in bold;
this coding portion starts at position 756 and ends at position
1844. The transcript also has the following SNPs as listed in Table
12 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T39971_P11 (SEQ ID NO:53) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00143 TABLE 12 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 417 G->C Yes 459 T->C Yes 1387 C->No 1406->A
No 1406->G No 1555 G->No 1555 G->C No 1558 G->No 1558
G->A Yes 1754 T->C Yes 1868 G->No 1868 G->T No 529
G->T Yes 1944 T->C Yes 1119 G->T Yes 1188 G->No 1295
C->No 1295 C->G No 1324->T No 1331 C->No 1381
C->No
[0797] Variant protein T39971_P12 (SEQ ID NO:54) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T39971_T16 (SEQ ID
NO:20). An alignment is given to the known protein (Vitronectin
precursor (SEQ ID NO:50)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[0798] Comparison report between T39971_P12 (SEQ ID NO:54) and
VTNC_HUMAN (SEQ ID NO:50):
[0799] 1. An isolated chimeric polypeptide encoding for T39971_P12
(SEQ ID NO:54), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to
amino acids 1-223 of VTNC_HUMAN (SEQ ID NO:50), which also
corresponds to amino acids 1-223 of T39971_P12 (SEQ ID NO:54), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence VPGAVGQGRKHLGRV (SEQ ID NO:1004) corresponding to
amino acids 224-238 of T39971_P12 (SEQ ID NO:54), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0800] 2. An isolated polypeptide encoding for a tail of T39971_P12
(SEQ ID NO:54), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO:1004) in
T39971_P12 (SEQ ID NO:54).
[0801] Comparison report between T39971_P12 (SEQ ID NO:54) and
Q9BSH7:
[0802] 1. An isolated chimeric polypeptide encoding for T39971_P12
(SEQ ID NO:54), comprising a first amino acid sequence being at
least 90% homologous to
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAEC
KPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPV
LKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFR
GQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFK corresponding to
amino acids 1-223 of Q9BSH7, which also corresponds to amino acids
1-223 of T39971_P12 (SEQ ID NO:54), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VPGAVGQGRKHLGRV (SEQ ID NO:1004) corresponding to amino acids
224-238 of T39971_P12 (SEQ ID NO:54), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0803] 2. An isolated polypeptide encoding for a tail of T39971_P12
(SEQ ID NO:54), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence VPGAVGQGRKHLGRV (SEQ ID NO:1004) in
T39971_P12 (SEQ ID NO:54).
[0804] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0805] Variant protein T39971_P12 (SEQ ID NO:54) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T39971_P 12 (SEQ ID NO:54)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-001 44 TABLE
13 Amino acid mutations SNP position(s) on amino acid Alternative
sequence amino acid(s) Previously known SNP? 122 A->S Yes 145
G->No 180 C->No 180 C->W No 192 Y->No 209A->No 211
T->No
[0806] Variant protein T39971_P12 (SEQ ID NO:54) is encoded by the
following transcript(s): T39971_T16 (SEQ ID NO:20), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T39971_T16 (SEQ ID NO:20) is shown in bold;
this coding portion starts at position 756 and ends at position
1469. The transcript also has the following SNPs as listed in Table
14 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T39971_P12 (SEQ ID NO:54) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00145 TABLE 14 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 417 G->C Yes 459 T->C Yes 1387 C->No 1406->A
No 1406->G No 529 G->T Yes 1119 G->T Yes 1188 G->No
1295 C->No 1295 C->G No 1324->T No 1331 C->No 1381
C->No
[0807] As noted above, cluster T39971 features 28 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[0808] Segment cluster T39971_node.sub.--0 (SEQ ID NO:22) according
to the present invention is supported by 76 libraries. The number
of libraries was determined as previously described. This segment
can be found in the following transcript(s): T39971_T1 (SEQ ID
NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16 (SEQ ID NO:20) and
T39971_T5 (SEQ ID NO:21). Table 15 below describes the starting and
ending position of this segment on each transcript. TABLE-US-00146
TABLE 15 Segment location on transcripts Segment Segment Transcript
name starting position ending position T39971_T10 (SEQ ID NO: 18) 1
810 T39971_T12 (SEQ ID NO: 19)1 810T39971_T16 (SEQ ID NO: 20)1
810T39971_T5 (SEQ ID NO: 21) 1 810
[0809] Segment cluster T39971_node.sub.--18 (SEQ ID NO:23)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T39971_T16
(SEQ ID NO:20). Table 16 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00147 TABLE
16 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T16 (SEQ ID NO: 20) 1425
1592
[0810] Segment cluster T39971_node.sub.--21 (SEQ ID NO:24)
according to the present invention is supported by 99 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19) and T39971_T5
(SEQ ID NO:21). Table 17 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00148 TABLE
17 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T10 (SEQ ID NO: 18) 1425
1581 T39971_T12 (SEQ ID NO: 19) 1425 1581 T39971_T5 (SEQ ID NO: 21)
1425 1581
[0811] Segment cluster T39971_node.sub.--22 (SEQ ID NO:25)
according to the present invention is supported by 7 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T39971_T5 (SEQ
ID NO:21). Table 18 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00149 TABLE
18 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T5 (SEQ ID NO: 21) 1582
1779
[0812] Segment cluster T39971_node.sub.--23 (SEQ ID NO:26)
according to the present invention is supported by 101 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19) and T39971_T5
(SEQ ID NO:21). Table 19 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00150 TABLE
19 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T10 (SEQ ID NO: 18) 1582
1734T39971_T12 (SEQ ID NO: 19) 1582 1734 T39971_T5 (SEQ ID NO: 21)
1780 1932
[0813] Segment cluster T39971_node.sub.--31 (SEQ ID NO:27)
according to the present invention is supported by 94 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18) and T39971_T5 (SEQ ID NO:21). Table 20
below describes the starting and ending position of this segment on
each transcript. TABLE-US-001 51 TABLE 20 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T39971_T10 (SEQ ID NO: 18) 1847 1986 T39971_T5 (SEQ
ID NO: 21) 2138 2277
[0814] Segment cluster T39971_node.sub.--33 (SEQ ID NO:28)
according to the present invention is supported by 77 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19) and T39971_T5
(SEQ ID NO:21). Table 21 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00152 TABLE
21 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T10 (SEQ ID NO: 18) 1987
2113 T39971_T12 (SEQ ID NO: 19)1735 1861 T39971_T5 (SEQ ID NO: 21)
2278 2404
[0815] Segment cluster T39971_node.sub.--7 (SEQ ID NO:29) according
to the present invention is supported by 87 libraries. The number
of libraries was determined as previously described. This segment
can be found in the following transcript(s): T39971_T11 (SEQ ID
NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16 (SEQ ID NO:20) and
T39971_T5 (SEQ ID NO:21). Table 22 below describes the starting and
ending position of this segment on each transcript. TABLE-US-00153
TABLE 22 Segment location on transcripts Segment Segment Transcript
name starting position ending position T39971_T10 (SEQ ID NO: 18)
940 1162 T39971_T12 (SEQ ID NO: 19) 940 1162 T39971_T16 (SEQ ID NO:
20) 940 1162 T39971_T5 (SEQ ID NO: 21) 940 1162
[0816] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[0817] Segment cluster T39971_node.sub.--1 (SEQ ID NO:30) according
to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 23 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00154 TABLE 23 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 811 819
T39971_T12 (SEQ ID NO: 19) 811 819 T39971_T16 (SEQ ID NO: 20) 811
819 T39971_T5 (SEQ ID NO: 21) 811 819
[0818] Segment cluster T39971_node.sub.--10 (SEQ ID NO:31)
according to the present invention is supported by 77 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16
(SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21). Table 24 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00155 TABLE 24 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 1189 1232 T39971_T12 (SEQ ID NO: 19)
1189 1232 T39971_T16 (SEQ ID NO: 20)1189 1232 T39971_T5 (SEQ ID NO:
21) 1189 1232
[0819] Segment cluster T39971_node.sub.--11 (SEQ ID NO:32)
according to the present invention is supported by 79 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16
(SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21). Table 25 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00156 TABLE 25 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 1233 1270 T39971_T12 (SEQ ID NO: 19)
1233 1270T39971_T16 (SEQ ID NO: 20) 1233 1270T39971_T5 (SEQ ID NO:
21) 1233 1270
[0820] Segment cluster T39971_node.sub.--12 (SEQ ID NO:33)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 26 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00157 TABLE 26 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 1271 1284
T39971_T12 (SEQ ID NO: 19) 1271 1284 T39971_T16 (SEQ ID NO: 20)
1271 1284 T39971_T5 (SEQ ID NO: 21) 1271 1284
[0821] Segment cluster T39971_node.sub.--15 (SEQ ID NO:34)
according to the present invention is supported by 79 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16
(SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21). Table 27 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00158 TABLE 27 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 1285 1316 T39971_T12 (SEQ ID NO: 19)
1285 1316 T39971_T16 (SEQ ID NO: 20)1285 1316 T39971_T5 (SEQ ID NO:
21) 1285 1316
[0822] Segment cluster T39971_node.sub.--16 (SEQ ID NO:35)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 28 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00159 TABLE 28 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 1317 1340
T39971_T12 (SEQ ID NO: 19) 1317 1340 T39971_T16 (SEQ ID NO: 20)
1317 1340 T39971_T5 (SEQ ID NO: 21) 1317 1340
[0823] Segment cluster T39971_node.sub.--17 (SEQ ID NO:36)
according to the present invention is supported by 86 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16
(SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21). Table 29 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00160 TABLE 29 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 1341 1424 T39971_T12 (SEQ ID NO: 19)
1341 1424T39971_T16 (SEQ ID NO: 20) 1341 1424T39971_T5 (SEQ ID NO:
21) 1341 1424
[0824] Segment cluster T39971_node.sub.--26 (SEQ ID NO:37)
according to the present invention is supported by 85 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): T39971_T5
(SEQ ID NO:21). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00161 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T5 (SEQ ID NO: 21) 1933
1974
[0825] Segment cluster T39971_node.sub.--27 (SEQ ID NO:38)
according to the present invention is supported by 90 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): T39971_T5
(SEQ ID NO:21). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00162 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T5 (SEQ ID NO: 21) 1975
2025
[0826] Segment cluster T39971_node.sub.--28 (SEQ ID NO:39)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18) and T39971_T5 (SEQ ID
NO:21). Table 32 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00163 TABLE 32 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 1735 1743
T39971_T5 (SEQ ID NO: 21) 2026 2034
[0827] Segment cluster T39971_node.sub.--29 (SEQ ID NO:40)
according to the present invention is supported by 99 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO 18) and T39971_T5 (SEQ ID NO:21). Table 33
below describes the starting and ending position of this segment on
each transcript. TABLE-US-001 64 TABLE 33 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T39971_T10 (SEQ ID NO: 18) 1744 1838 T39971_T5 (SEQ
ID NO: 21) 2035 2129
[0828] Segment cluster T39971_node.sub.--3 (SEQ ID NO:41) according
to the present invention is supported by 78 libraries. The number
of libraries was determined as previously described. This segment
can be found in the following transcript(s): T39971_T10 (SEQ ID
NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16 (SEQ ID NO:20) and
T39971_T5 (SEQ ID NO:21). Table 34 below describes the starting and
ending position of this segment on each transcript. TABLE-US-00165
TABLE 34 Segment location on transcripts Segment Segment Transcript
name starting position ending position T39971_T10 (SEQ ID NO: 18)
820 861 T39971_T12 (SEQ ID NO: 19) 820 861 T39971_T16 (SEQ ID NO:
20) 820 861 T39971_T5 (SEQ ID NO: 21) 820 861
[0829] Segment cluster T39971_node.sub.--30 (SEQ ID NO:42)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18) and T39971_T5 (SEQ ID
NO:21). Table 35 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00166 TABLE 35 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 1839 1846
T39971_T5 (SEQ ID NO: 21) 2130 2137
[0830] Segment cluster T39971_node.sub.--34 (SEQ ID NO:43)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19)
and T39971_T5 (SEQ ID NO:21). Table 36 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00167 TABLE 36 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 2114 2120 T39971_T12 (SEQ ID NO: 19)
1862 1868 T39971_T5 (SEQ ID NO: 21) 2405 2411
[0831] Segment cluster T39971_node.sub.--35 (SEQ ID NO:44)
according to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19)
and T39971_T5 (SEQ ID NO:21). Table 37 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00168 TABLE 37 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T39971_T10 (SEQ ID NO: 18) 2121 2137 T39971_T12 (SEQ ID NO: 19)
1869 1885 T39971_T5 (SEQ ID NO: 21) 2412 2428
[0832] Segment cluster T39971_node.sub.--36 (SEQ ID NO:45)
according to the present invention is supported by 51 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID NO:19) and T39971_T5
(SEQ ID NO:21). Table 38 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00169 TABLE
38 Segment location on transcripts Segment Segment Transcript name
starting position ending position T39971_T10 (SEQ ID NO: 18) 2138
2199 T39971_T12 (SEQ ID NO: 19) 1886 1947 T39971_T5 (SEQ ID NO: 21)
2429 2490
[0833] Segment cluster T39971_node.sub.--4 (SEQ ID NO:46) according
to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 39 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00170 TABLE 39 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T39971_T10 (SEQ ID NO: 18) 862 881
T39971_T12 (SEQ ID NO: 19) 862 881 T39971_T16 (SEQ ID NO: 20) 862
881 T39971_T5 (SEQ ID NO: 21) 862 881
[0834] Segment cluster T39971_node.sub.--5 (SEQ ID NO:47) according
to the present invention is supported by 80 libraries. The number
of libraries was determined as previously described. This segment
can be found in the following transcript(s): T39971_T10 (SEQ ID
NO:18), T39971_T12 (SEQ ID NO:19), T39971_T16 (SEQ ID NO:20) and
T39971_T5 (SEQ ID NO:21). Table 40 below describes the starting and
ending position of this segment on each transcript. TABLE-US-00171
TABLE 40 Segment location on transcripts Segment Segment ending
Transcript name starting position position T39971_T10 (SEQ ID NO:
18) 882 939 T39971_T12 (SEQ ID NO: 19) 882 939 T39971_T16 (SEQ ID
NO: 20) 882 939 T39971_T5 (SEQ ID NO: 21) 882 939
[0835] Segment cluster T39971_node.sub.--8 (SEQ ID NO:48) according
to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 41 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00172 TABLE 41 Segment
location on transcripts Segment Segment ending Transcript name
starting position T39971_T10 (SEQ ID NO: 18) 1163 1168 T39971_T12
(SEQ ID NO: 19) 1163 1168 T39971_T16 (SEQ ID NO: 20) 1163 1168
T39971_T5 (SEQ ID NO: 21) 1163 1168
[0836] Segment cluster T39971_node.sub.--9 (SEQ ID NO:49) according
to the present invention can be found in the following
transcript(s): T39971_T10 (SEQ ID NO:18), T39971_T12 (SEQ ID
NO:19), T39971_T16 (SEQ ID NO:20) and T39971_T5 (SEQ ID NO:21).
Table 42 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00173 TABLE 42 Segment
location on transcripts Segment Segment ending Transcript name
starting position T39971_T10 (SEQ ID NO: 18) 1169 1188 T39971_T12
(SEQ ID NO: 19) 1169 1188T39971_T16 (SEQ ID NO: 20) 1169
1188T39971_T5 (SEQ ID NO: 21) 1169 1188
[0837] Variant protein alignment to the previously known
protein:
[0838] Sequence name: /tmp/xkraCL20cZ/43L7YcPH7x:VTNC_HUMAN (SEQ ID
NO:50)
[0839] Sequence Documentation:
[0840] Alignment of: T39971_P6 (SEQ ID NO:51).times.VTNC_HUMAN (SEQ
ID NO:50).
[0841] Alignment segment 1/1: TABLE-US-00174 Quality: 2774.00
Escore: 0 Matching length: 278 Total length: 278 Matching Percent
99.64 Matching Percent Identity: 99.64 Similarity: Total Percent
Similarity: 99.64 Total Percent Identity: 99.64 Gaps: 0
TABLE-US-00007 Alignment: TABLE-US-00175 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250 . . . 251
PDNVDAALALPAHSYSGRERVYFFKGTQ 278 |||||||||||||||||||||||||| | 251
PDNVDAALALPAHSYSGRERVYFFKGKQ 278
[0842] Sequence name: /tmp/X4DeeuSIB4/yMubSR5FPs:VTNC_HUMAN (SEQ ID
NO:50)
[0843] Sequence Documentation:
[0844] Alignment of: T39971_P9 (SEQ ID NO:52).times.VTNC_HUMAN (SEQ
ID NO:50).
[0845] Alignment segment 1/1: TABLE-US-001 76 Quality: 4430.00
Escore: 0 Matching length: 447 Total length: 478 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 93.51 Total Percent Identity: 93.51 Gaps: 1
TABLE-US-00008 Alignment: TABLE-US-00177 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250 . . . 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300 . . . 301
VFEHFAMMQRDSWEDIFELLFWGRT......................... 325
||||||||||||||||||||||||| 301
VFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAM 350 . . . 326
......SGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAT 369
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
AGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAT 400 . . . 370
WLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLR 419
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
WLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLR 450 . . . 420
TRRVDTVDPPYPRSIAQYWLGCPAPGHL 447 |||||||||||||||||||||||||||| 451
TRRVDTVDPPYPRSIAQYWLGCPAPGHL 478
[0846] Sequence name: /tmp/jvp1VtnxNy/wxNSeFVZZw:VTNC_HUMAN (SEQ ID
NO:50)
[0847] Sequence Documentation:
[0848] Alignment of: T39971_P11 (SEQ ID NO:53).times.VTNC_HUMAN
(SEQ ID NO:50).
[0849] Alignment segment 1/1: TABLE-US-00178 Quality: 3576.00
Escore: 0 Matching length: 363 Total length: 478 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 75.94 Total Percent Identity: 75.94 Gaps: 1
TABLE-US-00009 Alignment: TABLE-US-00179 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
GIEGPIDAAFTRTNCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250 . . . 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300 . . . 301
VFEHFAMMQRDSWEDIFELLFWGRTS ........................ 326
|||||||||||||||||||||||||| 301
VFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAM 350 . . . 326
.................................................. 326 351
AGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAT 400 . . . 327
..........................................DKYYRVNLR 335 |||||||||
401 WLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLR 450 . . .
336 TRRVDTVDPPYPRSIAQYWLGCPAPGHL 363 ||||||||||||||||||||||||||||
451 TRRVDTVDPPYPRSIAQYWLGCPAPGHL 478
[0850] Sequence name: /tmp/jvp1VtnxNy/wxNSeFVZZw:Q9BSH7
[0851] Sequence Documentation:
[0852] Alignment of: T39971_P11 (SEQ ID NO:53).times.Q9BSH7
[0853] Alignment segment 1/1: TABLE-US-00180 Quality: 3576.00
Escore: 0 Matching length: 363 Total length: 478 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 75.94 Total Percent Identity: 75.94 Gaps: 1
TABLE-US-00010 Alignment: TABLE-US-00181 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
GIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGI 250 . . . 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
PDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSA 300 . . . 301
VFEHFAMMQRDSWEDIFELLFWGRTS ........................ 326
||||||||||||||||||||||||||| 301
VFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAM 350 . . . 326
.................................................... 326 351
AGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAM 400 . . . 327
.........................................DKYYRVNLR 335 |||||||||
401 WLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLR 450 . . .
336 TRRVDTVDPPYPRSIAQYWLGCPAPGHL 363 ||||||||||||||||||||||||||||
451 TRRVDTVDPPYPRSIAQYWLGCPAPGHL 478
[0854] Sequence name: /tmp/fgebv7ir4i/48bTBMziJ0:VTNC_HUMAN (SEQ ID
NO:50)
[0855] Sequence Documentation:
[0856] Alignment of: T39971_P12 (SEQ ID NO:54).times.VTNC_HUMAN
(SEQ ID NO:50).
[0857] Alignment segment 1/1: TABLE-US-00182 Quality: 2237.00
Escore: 0 Matching length: 223 Total length: 223 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00011 Alignment: TABLE-US-00183 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFK 223 ||||||||||||||||||||||| 201
GIEGPIDAAFTRINCQGKTYLFK 223
[0858] Sequence name: /tmp/fgebv7ir4i/48bTBMziJ0:Q9BSH7
[0859] Sequence Documentation:
[0860] Alignment of: T39971_P12 (SEQ ID NO:54).times.Q9BSH7
[0861] Alignment segment 1/1: TABLE-US-00184 Quality: 2237.00
Escore: 0 Matching length: 223 Total length: 223 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00012 Alignment: TABLE-US-00185 . . . 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSC 50 . . . 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
CTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTS 100 . . . 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
DLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPP 150 . . . 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
AEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVW 200 . . . 201
GIEGPIDAAFTRINCQGKTYLFK 223 ||||||||||||||||||||||| 201
GIEGPIDAAFTRINCQGKTYLFK 223
Expression of VTNC_HUMAN Vitronectin (Serum Spreading Factor,
Somatomedin B, Complement S-Protein) T39971 Transcripts, Which are
Detectable by Amplicon as Depicted in Sequence Name T39971
junc23-33 (SEQ ID NO:836) in Normal and Cancerous Breast
Tissues
[0862] Expression of VTNC_HUMAN vitronectin (serum spreading
factor, somatomedin B, complement S-protein) transcripts detectable
by or according to junc23-33, T39971 junc23-33 amplicon (SEQ ID
NO:836) and T39971 junc23-33F (SEQ ID NO:834) and T39971 junc23-33R
(SEQ ID NO:835) primers was measured by real time PCR. In parallel
the expression of four housekeeping genes--PBGD (GenBank Accession
No. BC019323 (SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID
NO:929), HPRT1 (GenBank Accession No. NM.sub.--000194 (SEQ ID
NO:930); amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)), and G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)), was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67, Table 1, above, "Tissue samples in testing panel"),
to obtain a value of fold differential expression for each sample
relative to median of the normal PM samples.
[0863] FIG. 10 is a histogram showing down regulation of the
above-indicated VTNC_HUMAN vitronectin (serum spreading factor,
somatomedin B, complement S-protein) transcripts in cancerous
breast samples relative to the normal samples.
[0864] As is evident from FIG. 10, the expression of VTNC_HUMAN
vitronectin (serum spreading factor, somatomedin B, complement
S-protein) transcripts detectable by the above amplicon in cancer
samples was significantly lower than in the non-cancerous samples
(Sample Nos. 56-60, 63-67 Table 1, "Tissue samples in testing
panel").
[0865] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: T39971
junc23-33F (SEQ ID NO:834) forward primer; and T39971 junc23-33R
(SEQ ID NO:835) reverse primer.
[0866] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: T39971 junc23-33 (SEQ ID NO:836). TABLE-US-00186
T39971junc22-33F (SEQ ID NO: 834): GGGGCAGAACCTCTGACAAG
T39971junc22-33R (SEQ ID NO: 835): GGGCAGCCCAGCCAGTA
T39971junc22-33 amplicon (SEQ ID NO: 836):
GGGGCAGAACCTCTGACAAGTACTACCGAGTCAATCTTCGCACACGGCGAGTGGAC
ACTGTGGACCCTCCCTACCCACGCTCCATCGCTCAGTACTGGCTGGGCTGCCC
Expression of VTNC_HUMAN Vitronectin (Serum Spreading Factor,
Somatomedin B, Complement S-Protein), Antisense to SARM1 (T23434),
T39971 Transcripts Which are Detectable by Amplicon as Depicted in
Sequence Name T39971junc23-33 (SEQ ID NO:836) in Different Normal
Tissues
[0867] Expression of VTNC_HUMAN vitronectin (serum spreading
factor, somatomedin B, complement S-protein), transcripts
detectable by or according to T39971junc23-33 amplicon (SEQ ID
NO:836) and T39971junc23-33F (SEQ ID NO:834) and T39971junc23-33R
(SEQ ID NO:835) was measured by real time PCR. In parallel the
expression of four housekeeping genes-RPL19 (GenBank Accession No.
NM.sub.--000981 (SEQ ID NO:934); RPL19 amplicon (SEQ ID NO:937)),
TATA box (GenBank Accession No. NM.sub.--003194 (SEQ ID NO:938);
TATA amplicon (SEQ ID NO:941)), UBC (GenBank Accession No. BC000449
(SEQ ID NO:942); amplicon--Ubiquitin-amplicon (SEQ ID NO:945)) and
SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the breast samples
(Sample Nos. 33-35, Table 2, "Tissue samples in normal panel"
above), to obtain a value of relative expression of each sample
relative to median of the breast samples. Primers and amplicon are
as above.
[0868] The results are presented in FIG. 11, demonstrating the
expression of VTNC_HUMAN vitronectin (serum spreading factor,
somatomedin B, complement S-protein), antisense to SARM1 (T23434),
T39971 transcripts, which are detectable by amplicon as depicted in
sequence name T39971junc23-33 (SEQ ID NO:836), in different normal
tissues.
Expression of VTNC_HUMAN Vitronectin (Serum Spreading Factor,
Somatomedin B, Complement S-Protein) T39971 Transcripts Which are
Detectable by Amplicon as Depicted in Sequence Name T39971 Seg22
(SEQ ID NO:839) in Normal and Cancerous Breast Tissues
[0869] Expression of VTNC_HUMAN vitronectin (serum spreading
factor, somatomedin B, complement S-protein) transcripts detectable
by or according to seg22, T39971 seg22 (SEQ ID NO:839) amplicon(s)
and primers T39971 seg22F (SEQ ID NO:837) and T39971 seg22R (SEQ ID
NO:838) was measured by real time PCR. In parallel the expression
of four housekeeping genes-PBGD (GenBank Accession No. BC019323
(SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1
(GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925)); G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID
NO:918); G6PD-amplicon (SEQ ID NO:921)), was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the normal
post-mortem (PM) samples (Sample Nos. 56-60, 63-67, Table 1: Tissue
samples in testing panel, above), to obtain a value of fold
differential expression for each sample relative to median of the
normal PM samples.
[0870] In one experiment that was carried out no differential
expression in the cancerous samples relative to the normal PM
samples was observed. However, this may be due to a problem that is
specific to this particular experiment.
[0871] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: T39971 seg22F
(SEQ ID NO:837) forward primer; and T39971 seg22R (SEQ ID NO:838)
reverse primer.
[0872] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: T39971 seg22 (SEQ ID NO:839). TABLE-US-00187 Forward
primer T39971 seg22F: (SEQ ID NO: 837) GCAGTCTTGGATTCCTTTCACATT
Reverse primer T39971 seg22R: (SEQ ID NO: 838)
GAGGCTGTTGAAGTTAGGATCTCC Amplicon T39971 seg22: (SEQ ID NO: 839)
GCAGTCTTGGATTCCTTTCACATTTCACTGGGGACAGGCCTCAGCATGTGCCCACCC
CTGACCCCCACCTCATGCTGGGAGATCCTAACTTCAACAGCCTC
Description for Cluster Z21368
[0873] Cluster Z21368 features 7 transcript(s) and 34 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00188 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. Z21368_PEA.sub.--1_T10 55 Z21368_PEA.sub.--1_T11 56
Z21368_PEA.sub.--1_T23 57 Z21368_PEA.sub.--1_T24 58
Z21368_PEA.sub.--1_T5 59 Z21368_PEA.sub.--1_T6 60
Z21368_PEA.sub.--1_T9 61
[0874] TABLE-US-00189 TABLE 2 Segments of interest Segment Name
Sequence ID No. Z21368_PEA.sub.--1_node.sub.--0 62
Z21368_PEA.sub.--1_node.sub.--15 63
Z21368_PEA.sub.--1_node.sub.--19 64 Z21368_PEA.sub.--1_node.sub.--2
65 Z21368_PEA.sub.--1_node.sub.--21 66
Z21368_PEA.sub.--1_node.sub.--33 67
Z21368_PEA.sub.--1_node.sub.--36 68
Z21368_PEA.sub.--1_node.sub.--37 69
Z21368_PEA.sub.--1_node.sub.--39 70 Z21368_PEA.sub.--1_node.sub.--4
71 Z21368_PEA.sub.--1_node.sub.--41 72
Z21368_PEA.sub.--1_node.sub.--43 73
Z21368_PEA.sub.--1_node.sub.--45 74
Z21368_PEA.sub.--1_node.sub.--53 75
Z21368_PEA.sub.--1_node.sub.--56 76
Z21368_PEA.sub.--1_node.sub.--58 77
Z21368_PEA.sub.--1_node.sub.--66 78
Z21368_PEA.sub.--1_node.sub.--67 79
Z21368_PEA.sub.--1_node.sub.--69 80
Z21368_PEA.sub.--1_node.sub.--11 81
Z21368_PEA.sub.--1_node.sub.--12 82
Z21368_PEA.sub.--1_node.sub.--16 83
Z21368_PEA.sub.--1_node.sub.--17 84
Z21368_PEA.sub.--1_node.sub.--23 85
Z21368_PEA.sub.--1_node.sub.--24 86
Z21368_PEA.sub.--1_node.sub.--30 87
Z21368_PEA.sub.--1_node.sub.--31 88
Z21368_PEA.sub.--1_node.sub.--38 89
Z21368_PEA.sub.--1_node.sub.--47 90
Z21368_PEA.sub.--1_node.sub.--49 91
Z21368_PEA.sub.--1_node.sub.--51 92
Z21368_PEA.sub.--1_node.sub.--61 93
Z21368_PEA.sub.--1_node.sub.--68 94
Z21368_PEA.sub.--1_node.sub.--795
[0875] TABLE-US-00190 TABLE 3 Proteins of interest Protein Name
Sequence ID No. Z21368_PEA.sub.--1_P2 97 Z21368_PEA.sub.--1_P5 98
Z21368_PEA.sub.--1_P15 99 Z21368_PEA.sub.--1_P16 100
Z21368_PEA.sub.--1_P22 101 Z21368_PEA.sub.--1_P23 102
[0876] These sequences are variants of the known protein
Extracellular sulfatase Sulf-1 precursor (SwissProt accession
identifier SUL1_HUMAN; known also according to the synonyms EC
3.1.6.-; HSulf-1), SEQ ID NO:96, referred to herein as the
previously known protein.
[0877] Protein Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96) is known or believed to have the following function(s):
Exhibits arylsulfatase activity and highly specific
endoglucosamine-6-sulfatase activity. It can remove sulfate from
the C-6 position of glucosamine within specific subregions of
intact heparin. Diminishes HSPG (heparan sulfate proteoglycans)
sulfation, inhibits signaling by heparin-dependent growth factors,
diminishes proliferation, and facilitates apoptosis in response to
exogenous stimulation. The sequence for protein Extracellular
sulfatase Sulf-1 precursor is given at the end of the application,
as "Extracellular sulfatase Sulf-1 precursor amino acid sequence"
(SEQ ID NO:96). Known polymorphisms for this sequence are as shown
in Table 4. TABLE-US-001 91 TABLE 4 Amino acid mutations for Known
Protein SNP position(s) on amino acid sequence Comment 87-88
CC->AA: LOSS OF ARYLSULFATASE ACTIVITY AND LOSS OF ABILITY TO
MODULATE APOPTOSIS. 49 L->P 728 K->R
[0878] Protein Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96) localization is believed to be Endoplasmic reticulum and
Golgi stack; also localized on the cell surface (By
similarity).
[0879] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: apoptosis;
metabolism; heparan sulfate proteoglycan metabolism, which are
annotation(s) related to Biological Process; arylsulfatase;
hydrolase, which are annotation(s) related to Molecular Function;
and extracellular space; endoplasmic reticulum; Golgi apparatus,
which are annotation(s) related to Cellular Component.
[0880] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[0881] Cluster Z21368 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the right hand column of the table and the numbers on the y-axis of
FIG. 12 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[0882] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 12 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues and pancreas carcinoma.
TABLE-US-00192 TABLE 5 Normal tissue distribution Name of Tissue
Number bladder 123 Bone 557 Brain 34 Colon 94 epithelial 56 general
68 head and neck 0 kidney 35 Lung 22 lymph nodes 0 breast 52 muscle
31 ovary 0 pancreas 0 prostate 44 skin 67 stomach 109 T cells 0
Thyroid 0 uterus 140
[0883] TABLE-US-001 93 TABLE 6 P values and ratios for expression
in cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 bladder
5.4e-01 6.6e-01 6.4e-01 1.0 8.5e-01 0.7 bone 4.5e-01 8.2e-01
9.1e-01 0.4 1 0.3 brain 5.5e-01 7.3e-01 1.5e-01 1.5 5.0e-01 0.9
colon 1.4e-01 2.8e-01 1.0e-01 2.0 3.0e-01 1.4 epithelial 1.1e-03
1.5e-01 1.2e-07 2.1 1.0e-01 1.1 general 1.4e-05 5.3e-02 1.9e-06 1.6
6.7e-01 0.8 head and neck 2.4e-02 7.1e-02 4.6e-01 2.5 7.5e-01 1.4
kidney 8.9e-01 9.0e-01 1 0.4 1 0.4 lung 3.5e-01 4.1e-01 7.2e-03 2.6
1.0e-01 1.6 lymph nodes 7.7e-02 3.1e-01 2.3e-02 8.5 1.9e-01 3.2
breast 4.0e-01 6.1 e-01 5.4e-02 2.3 3.0e-01 1.3 muscle 7.5e-02
3.5e-02 1 1.0 1.7e-01 1.7 ovary 3.8e-01 4.2e-01 2.2e-01 2.9 3.4e-01
2.2 pancreas 2.2e-02 6.9e-02 1.4e-08 6.5 1.4e-06 4.6 prostate
8.3e-01 8.9e-01 3.1e-01 1.4 5.2e-01 1.1 skin 6.1e-01 8.1e-01
6.0e-01 1.2 1 0.3 stomach 4.4e-02 5.0e-01 5.0e-01 0.8 9.7e-01 0.4 T
cells 5.0e-01 6.7e-01 3.3e-01 3.1 7.2e-01 1.4 Thyroid 3.6e-01
3.6e-01 1 1.1 1 1.1 uterus 3.5e-01 7.8e-01 4.6e-01 0.9 9.1e-01
0.5
[0884] As noted above, cluster Z21368 features 7 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Extracellular sulfatase
Sulf-1 precursor (SEQ ID NO:96). A description of each variant
protein according to the present invention is now provided.
[0885] Variant protein Z21368_PEA.sub.--1_P2 (SEQ ID NO:97)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59). An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96) at the end of the application. One or more alignments to one
or more previously published protein sequences are given at the end
of the application. A brief description of the relationship of the
variant protein according to the present invention to each such
aligned protein is as follows:
[0886] Comparison report between Z21368_PEA.sub.--1_P2 (SEQ ID
NO:97) and SUL1_HUMAN (SEQ ID NO:96):
[0887] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFL
VERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGK
LRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNO
GTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQ
ASSGGNRGRMLADSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYI
DKEIEALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKE
AAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWN
corresponding to amino acids 1-761 of SUL1_HUMAN (SEQ ID NO:96),
which also corresponds to amino acids 1-761 of
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
PHKYSAHGRTRHFESATRTTNGAQKLSRI (SEQ ID NO:1005) corresponding to
amino acids 762-790 of Z21368_PEA.sub.--1_P2 (SEQ ID NO:97),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[0888] 2. An isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
PHKYSAHGRTRHFESATRTTNGAQKLSRI (SEQ ID NO:1005) in
Z21368_PEA.sub.--1_P2 (SEQ ID NO:97).
[0889] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0890] Variant protein Z21368_PEA.sub.--1_P2 (SEQ ID NO:97) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript Z21368_PEA.sub.--1_T5
(SEQ ID NO:59) is shown in bold; this coding portion starts at
position 529 and ends at position 2898.
[0891] Variant protein Z21368_PEA.sub.--1_P5 (SEQ ID NO:98)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[0892] Comparison report between Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98) and Q7Z2W2 (SEQ ID NO:840) (SEQ ID NO:840):
[0893] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVEL
corresponding to amino acids 1-57 of Q7Z2W2 (SEQ ID NO:840), which
also corresponds to amino acids 1-57 of Z21368_PEA.sub.--1_P5 (SEQ
ID NO:98), second bridging amino acid sequence comprising A, and a
third amino acid sequence being at least 90% homologous to
FFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITN
ESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNM
DKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYT
ADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDT
PPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHL
PKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLY
ARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNOGTPKYKPRFVHTRQTRSLSVEFE
GEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPT
TVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKR
RKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRKKER
KEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNE
THNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNOLHVQLMELRSCQGYKQCN
PRPKNLDVGNKDGGSYDLHRGQLWDGWEG corresponding to amino acids 139-871
of Q7Z2W2 (SEQ ID NO:840), which also corresponds to amino acids
59-791 of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein said first,
second and third amino acid sequences are contiguous and in a
sequential order.
[0894] 2. An isolated polypeptide encoding for an edge portion of
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least three amino
acids comprise LAF having a structure as follows (numbering
according to Z21368_PEA.sub.--1_P5 (SEQ ID NO:98)): a sequence
starting from any of amino acid numbers 57-x to 57; and ending at
any of amino acid numbers 59+((n-2)-x), in which x varies from 0 to
n-2.
[0895] Comparison report between Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98) and AAH12997 (SEQ ID NO:841) (SEQ ID NO:841):
[0896] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELAFF
GKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNES
INYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDK
HWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTAD
HGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPP
DVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLP
KYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYA
RGFHDKDKECSCRESGYRASRSQRKSQRQFLRNOGTPKYKPRFVHTRQTRSLSVEFEGE
IYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTV
RVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRK
PEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEMQEVDSKLQLFKENNRRRKKERKE
KRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETH
NFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNOLHVQLME (SEQ ID NO:1006)
corresponding to amino acids 1-751 of Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98), and a second amino acid sequence being at least 90%
homologous to LRSCQGYKQCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG
corresponding to amino acids 1-40 of AAH12997 (SEQ ID NO:841),
which also corresponds to amino acids 752-791 of
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein said first and second
amino acid sequences are contiguous and in a sequential order.
[0897] 2. An isolated polypeptide encoding for a head of
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00194
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELAFF (SEQ
ID NO: 1006)
GKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNES
INYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDK
HWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTAD
HGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPP
DVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLP
KYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYA
RGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGE
IYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTV
RVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRK
PEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEMQEVDSKLQLFKENNRRRKKERKE
KRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETH
NFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLME of
Z21368_PEA.sub.--1_P5. (SEQ ID NO: 98)
[0898] Comparison report between Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98) and SUL1_HUMAN (SEQ ID NO:96):
[0899] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a first amino acid
sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVEL
corresponding to amino acids 1-57 of SUL1_HUMAN (SEQ ID NO:96),
which also corresponds to amino acids 1-57 of Z21368_PEA.sub.--1_P5
(SEQ ID NO:98), and a second amino acid sequence being at least 90%
homologous to
AFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLIT
NESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQFSKLYPNASQHITPSYNYAPN
MDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYII
YTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGL
DTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSN
HLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRN
LYARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNOGTPKYKPRFVHTRQTRSLSVE
FEGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGP
PTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHL
KRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRK
KERKEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRT
VNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNOLHVQLMELRSCQGYK
QCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG corresponding to amino acids
138-871 of SUL1_HUMAN (SEQ ID NO:96), which also corresponds to
amino acids 58-791 of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), wherein
said first and second amino acid sequences are contiguous and in a
sequential order.
[0900] 2. An isolated chimeric polypeptide encoding for an edge
portion of Z21368_PEA.sub.--1_P5 (SEQ ID NO:98), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise LA, having a structure as follows: a
sequence starting from any of amino acid numbers 57-x to 57; and
ending at any of amino acid numbers 58+((n-2)-x), in which x varies
from 0 to n-2.
[0901] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0902] Variant protein Z21368_PEA.sub.--1_P5 (SEQ ID NO:98) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T9 (SEQ
ID NO:61), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript Z21368_PEA.sub.--1_T9
(SEQ ID NO:61) is shown in bold; this coding portion starts at
position 556 and ends at position 2928.
[0903] Variant protein Z21368_PEA.sub.--1_P15 (SEQ ID NO:99)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T23 (SEQ ID NO:57). An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[0904] Comparison report between Z21368_PEA.sub.--1_P15 (SEQ ID
NO:99) and SUL1_HUMAN (SEQ ID NO:96):
[0905] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P15 (SEQ ID NO. 99), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFL VERG
corresponding to amino acids 1-416 of SUL1_HUMAN (SEQ ID NO:96),
which also corresponds to amino acids 1-416 of
Z21368_PEA.sub.--1_P15 (SEQ ID NO:99).
[0906] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0907] Variant protein Z21368_PEA.sub.--1_P15 (SEQ ID NO:99) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript
Z21368_PEA.sub.--1_T23 (SEQ ID NO:57) is shown in bold; this coding
portion starts at position 691 and ends at position 1938.
[0908] Variant protein Z21368_PEA.sub.--1_P16 (SEQ ID NO:100)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T24 (SEQ ID NO:58). An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[0909] Comparison report between Z21368_PEA.sub.--1_P16 (SEQ ID
NO:100) and SUL1_HUMAN (SEQ ID NO:96):
[0910] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P16 (SEQ ID NO:100), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHMPHGPEDSAPQ
FSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDD
SVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEP
GSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNR corresponding to
amino acids 1-397 of SUL1_HUMAN (SEQ ID NO:96), which also
corresponds to amino acids 1-397 of Z21368_PEA.sub.--1_P 16 (SEQ ID
NO:100), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence CVIVPPLSQPQIH (SEQ ID NO:1007)
corresponding to amino acids 398-410 of Z21368_PEA.sub.--1_P16 (SEQ
ID NO:100), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[0911] 2. An isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P 16 (SEQ ID NO:100), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
CVIVPPLSQPQIH (SEQ ID NO:1007) in Z21368_PEA.sub.--1_P16 (SEQ ID
NO:100).
[0912] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0913] Variant protein Z21368_PEA.sub.--1_P16 (SEQ ID NO:100) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T24 (SEQ
ID NO:58), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript
Z21368_PEA.sub.--1_T24 (SEQ ID NO:58) is shown in bold; this coding
portion starts at position 691 and ends at position 1920.
[0914] Variant protein Z21368_PEA.sub.--1_P22 (SEQ ID NO:101)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T10 (SEQ ID NO:55) An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96) at the end of the application. One or more alignments to one
or more previously published protein sequences are given at the end
of the application. A brief description of the relationship of the
variant protein according to the present invention to each such
aligned protein is as follows:
[0915] Comparison report between Z21368_PEA.sub.--1_P22 (SEQ ID
NO:101) and SUL1_HUMAN (SEQ ID NO:96):
[0916] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCR
NGIKEKHGFDYAK corresponding to amino acids 1-188 of SUL1_HUMAN (SEQ
ID NO:96), which also corresponds to amino acids 1-188 of
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ARYDGDQPRCAPRPRGLSPTVF (SEQ ID NO:1008) corresponding to amino
acids 189-210 of Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), wherein
said first and second amino acid sequences are contiguous and in a
sequential order.
[0917] 2. An isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P22 (SEQ ID NO:101), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ARYDGDQPRCAPRPRGLSPTVF (SEQ ID NO:1008) in Z21368_PEA.sub.--1_P22
(SEQ ID NO:101).
[0918] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0919] Variant protein Z21368_PEA.sub.--1_P22 (SEQ ID NO:101) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T10 (SEQ
ID NO:55), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript
Z21368_PEA.sub.--1_T10 (SEQ ID NO:55) is shown in bold; this coding
portion starts at position 691 and ends at position 1320.
[0920] Variant protein Z21368_PEA.sub.--1_P23 (SEQ ID NO:102)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z21368_PEA.sub.--1_T10 (SEQ ID NO:56). An alignment is given to the
known protein (Extracellular sulfatase Sulf-1 precursor (SEQ ID
NO:96)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[0921] Comparison report between Z21368_PEA.sub.--1_P23 (SEQ ID
NO:102) and Q7Z2W2 (SEQ ID NO:840):
[0922] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRT corresponding to amino acids 1-137 of Q7Z2W2
(SEQ ID NO:840), which also corresponds to amino acids 1-137 of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence GLLHRLNH
(SEQ ID NO:1009) corresponding to amino acids 138-145 of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[0923] 2. An isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GLLHRLNH
(SEQ ID NO:1009) in Z21368_PEA.sub.--1_P23 (SEQ ID NO:102).
[0924] Comparison report between Z21368_PEA.sub.--1_P23 (SEQ ID
NO:102) and SUL1_HUMAN (SEQ ID NO:96):
[0925] 1. An isolated chimeric polypeptide encoding for
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a first amino
acid sequence being at least 90% homologous to
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSL
QVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSW
QAMHEPRTFAVYLNNTGYRT corresponding to amino acids 1-137 of
SUL1_HUMAN (SEQ ID NO:96), which also corresponds to amino acids
1-137 of Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence GLLHRLNH (SEQ ID NO:1009) corresponding to amino acids
138-145 of Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[0926] 2. An isolated polypeptide encoding for a tail of
Z21368_PEA.sub.--1_P23 (SEQ ID NO:102), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GLLHRLNH
(SEQ ID NO:1009) in Z21368_PEA.sub.--1_P23 (SEQ ID NO:102).
[0927] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[0928] Variant protein Z21368_PEA.sub.--1_P23 (SEQ ID NO: 102) is
encoded by the following transcript(s): Z21368_PEA.sub.--1_T10 (SEQ
ID NO:56), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript
Z21368_PEA.sub.---1_T11 (SEQ ID NO:56) is shown in bold; this
coding portion starts at position 691 and ends at position
1125.
[0929] As noted above, cluster Z21368 features 34 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[0930] Segment cluster Z21368_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:62) according to the present invention is supported by 8
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 7 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00195 TABLE 7 Segment location on transcripts
Segment Segment ending Transcript name starting position position
Z21368_PEA.sub.--1_T9 (SEQ ID 1 327 NO: 61)
[0931] Segment cluster Z21368_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:63) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 8 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00196 TABLE 8 Segment location on transcripts
Segment Segment ending Transcript name starting position position
Z21368_PEA.sub.--1_T10 (SEQ 631 807 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 631 807 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 631 807 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 631 807 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 469 645 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 469 645 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 496 672 NO:
61)
[0932] Segment cluster Z21368_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:64) according to the present invention is supported by 24
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59) and Z21368_PEA.sub.--1_T6 (SEQ
ID NO:60). Table 9 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00197 TABLE 9 Segment
location on transcripts Segment Segment ending Transcript name
starting position Z21368_PEA.sub.--1_T10 (SEQ 863 1102 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 863 1102 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 863 1102 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 863 1102 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 701 940 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 701 940 NO: 60)
[0933] Segment cluster Z21368_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:65) according to the present invention is supported by 15
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59) and Z21368_PEA.sub.--1_T6 (SEQ
ID NO:60). Table 10 below describes the starting and ending
position of this segment on each transcript. TABLE-US-001 98 TABLE
10 Segment location on transcripts Segment Segment ending
Transcript name starting position Z21368_PEA.sub.--1_T10 (SEQ 1 300
ID NO: 55) Z21368_PEA.sub.--1_T11 (SEQ 1 300 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 1 300 ID NO: 57) Z21368_PEA.sub.--1_T24
(SEQ 1 300 ID NO: 58) Z21368_PEA.sub.--1_T5 (SEQ ID 1 300 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1 300 NO: 60)
[0934] Segment cluster Z21368_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:66) according to the present invention is supported by 37
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T23 (SEQ ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ
ID NO:58), Z21368_PEA.sub.--1_T5 (SEQ ID NO:59),
Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and Z21368_PEA.sub.--1_T9 (SEQ
ID NO:61). Table 11 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00199 TABLE
11 Segment location on transcripts Segment Segment ending
Transcript name starting position Z21368_PEA.sub.--1_T10 (SEQ 1103
1254 ID NO: 55) Z21368_PEA.sub.--1_T23 (SEQ 1103 1254 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 1103 1254 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 941 1092 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 941 1092 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 728 879 NO: 61)
[0935] Segment cluster Z21368_PEA.sub.--1_node.sub.--33 (SEQ ID
NO:67) according to the present invention is supported by 45
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 12 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00200 TABLE 12 Segment location on transcripts
Segment Segment ending Transcript name starting position position
Z21368_PEA.sub.--1_T10 (SEQ 1502 1677 ID NO: 55) Z21368_PEA.sub.--1
T11 (SEQ 1424 1599 ID NO: 56) Z21368_PEA.sub.--1_T23 (SEQ 1576 1751
ID NO: 57) Z21368_PEA.sub.--1_T24 (SEQ 1576 1751 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1414 1589 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1414 1589 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1201 1376 NO: 61)
[0936] Segment cluster Z21368_PEA.sub.--1_node.sub.--36 (SEQ ID
NO:68) according to the present invention is supported by 44
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 13 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00201 TABLE 13 Segment location on transcripts
Segment Segment ending Transcript name starting position position
Z21368_PEA.sub.--1_T10 (SEQ 1678 1806 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 1600 1728 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 1752 1880 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 1752 1880 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1590 1718 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1590 1718 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1377 1505 NO: 61)
[0937] Segment cluster Z21368_PEA.sub.--1_node.sub.--37 (SEQ ID
NO:69) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T24 (SEQ ID NO:58). Table 14
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00202 TABLE 14 Segment location on
transcripts Segment Transcript name starting position Segment
ending position Z21368_PEA.sub.--1_T24 1881 2159 (SEQ ID NO:
58)
[0938] Segment cluster Z21368_PEA.sub.--1_node.sub.--39 (SEQ ID
NO:70) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T23 (SEQ ID NO:57) and
Z21368_PEA.sub.--1 T24 (SEQ ID NO:58). Table 15 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00203 TABLE 15 Segment location on transcripts Segment
Transcript name Segment starting position ending position
Z21368_PEA.sub.--1_T23 (SEQ 1938 2790 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 2217 3069 ID NO: 58)
[0939] Segment cluster Z21368_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:71) according to the present invention is supported by 13
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57) and Z21368_PEA.sub.--1_T24 (SEQ ID NO:58). Table 16 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00204 TABLE 16 Segment location on transcripts
Segment Transcript name Segment starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 301 462 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 301 462 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 301 462 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 301 462 ID NO: 58)
[0940] Segment cluster Z21368_PEA.sub.--1_node.sub.--41 (SEQ ID
NO:72) according to the present invention is supported by 49
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 17 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00205 TABLE 17 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1864 1993 ID NO: 55) Z21368_PEA 1_T11
(SEQ 1786 1915 ID NO: 56) Z21368_PEA.sub.--1_T5 (SEQ ID 1776 1905
NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID 1776 1905 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1563 1692 NO: 61)
[0941] Segment cluster Z21368_PEA.sub.--1_node.sub.--43 (SEQ ID
NO:73) according to the present invention is supported by 52
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 18 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00206 TABLE 18 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1994 2210 ID NO: 55) Z21368_PEA 1_T11
(SEQ 1916 2132 ID NO: 56) Z21368_PEA.sub.--1_T5 (SEQ ID 1906 2122
NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID 1906 2122 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1693 1909 NO: 61)
[0942] Segment cluster Z21368_PEA.sub.--1_node.sub.--45 (SEQ ID
NO:74) according to the present invention is supported by 64
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 19 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00207 TABLE 19 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 2211 2466 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 2133 2388 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 2123 2378 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 2123 2378 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1910 2165 NO: 61)
[0943] Segment cluster Z21368_PEA.sub.--1_node.sub.--53 (SEQ ID
NO:75) according to the present invention is supported by 60
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 20 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00208 TABLE 20 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 2725 2900 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 2647 2822 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 2637 2812 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 2637 2812 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2424 2599 NO: 61)
[0944] Segment cluster Z21368_PEA.sub.--1_node.sub.--56 (SEQ ID
NO:76) according to the present invention is supported by 50
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56) and Z21368_PEA.sub.--1_T9
(SEQ ID NO:61). Table 21 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00209 TABLE
21 Segment location on transcripts Segment Segment Transcript name
starting position ending position Z21368_PEA.sub.--1_T10 (SEQ 2901
3043 ID NO: 55) Z21368_PEA.sub.--1_T11 (SEQ 2823 2965 ID NO: 56)
Z21368_PEA.sub.--1_T9 (SEQ 2600 2742 ID NO: 61)
[0945] Segment cluster Z21368_PEA.sub.--1_node.sub.--58 (SEQ ID
NO:77) according to the present invention is supported by 71
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 22 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-0021 0 TABLE 22 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 3044 3167 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 2966 3089 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 2813 2936 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 2813 2936 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2743 2866 NO: 61)
[0946] Segment cluster Z21368_PEA.sub.--1_node.sub.--66 (SEQ ID
NO:78) according to the present invention is supported by 142
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56, Z21368_PEA.sub.--1_T5 (SEQ ID
NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 23 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00211 TABLE 23 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 3202 3789 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 3124 3711 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 2971 3558 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 2971 3558 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2901 3488 NO: 61)
[0947] Segment cluster Z21368_PEA.sub.--1_node.sub.--67 (SEQ ID
NO:79) according to the present invention is supported by 181
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 24 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00212 TABLE 24 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 3790 4374 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 3712 4296 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 3559 4143 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 3559 4143 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 3489 4073 NO: 61)
[0948] Segment cluster Z21368_PEA.sub.--1_node.sub.--69 (SEQ ID
NO:80) according to the present invention is supported by 150
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 25 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00213 TABLE 25 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 4428 4755 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 4350 4677 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 4197 5384 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 4197 4524 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 4127 4454 NO: 61)
[0949] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[0950] Segment cluster Z21368_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:81) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 26 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00214 TABLE 26 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 558 602 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 558 602 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 558 602 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 558 602 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 396 440 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 396 440 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 423 467 NO:
61)
[0951] Segment cluster Z21368_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:82) according to the present invention is supported by 23
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56, Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1T9 (SEQ ID NO:61). Table 27 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00215 TABLE 27 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 603 630 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 603 630 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 603 630 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 603 630 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 441 468 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 441 468 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 468 495 NO:
61)
[0952] Segment cluster Z21368_PEA.sub.--1_node.sub.--16 (SEQ ID
NO:83) according to the present invention can be found in the
following transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 28 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00216 TABLE 28 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 808 822 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 808 822 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 808 822 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 808 822 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 646 660 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 646 660 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 673 687 NO:
61)
[0953] Segment cluster Z21368_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:84) according to the present invention is supported by 19
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 29 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00217 TABLE 29 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 823 862 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 823 862 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 823 862 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 823 862 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 661 700 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 661 700 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 688 727 NO:
61)
[0954] Segment cluster Z21368_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:85) according to the present invention is supported by 36
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T11 (SEQ ID NO:56),
Z21368_PEA.sub.--1_T23 (SEQ ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ
ID NO:58), Z21368_PEA.sub.--1_T5 (SEQ ID NO:59),
Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and Z21368_PEA.sub.--1_T9 (SEQ
ID NO:61). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00218 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position Z21368_PEA.sub.--1_T11 (SEQ 1103
1176 ID NO: 56) Z21368_PEA.sub.--1_T23 (SEQ 1255 1328 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 1255 1328 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1093 1166 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1093 1166 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 880 953 NO: 61)
[0955] Segment cluster Z21368_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:86) according to the present invention is supported by 36
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 31 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00219 TABLE 31 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1255 1350 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 1177 1272 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 1329 1424 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 1329 1424 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1167 1262 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1167 1262 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 954 1049 NO: 61)
[0956] Segment cluster Z21368_PEA.sub.--1_node.sub.--30 (SEQ ID
NO:87) according to the present invention is supported by 39
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 32 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00220 TABLE 32 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1351 1409 ID NO: 55) Z21368_PEA 1 T11
(SEQ 1273 1331 ID NO: 56) Z21368_PEA.sub.--1_T23 (SEQ 1425 1483 ID
NO: 57) Z21368_PEA.sub.--1_T24 (SEQ 1425 1483 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1263 1321 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1263 1321 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1050 1108 NO: 61)
[0957] Segment cluster Z21368_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:88) according to the present invention is supported by 40
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 33 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00221 TABLE 33 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1410 1501 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 1332 1423 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 1484 1575 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 1484 1575 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1322 1413 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1322 1413 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1109 1200 NO: 61)
[0958] Segment cluster Z21368_PEA.sub.--1_node.sub.--38 (SEQ ID
NO:89) according to the present invention is supported by 45
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 34 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00222 TABLE 34 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 1807 1863 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 1729 1785 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 1881 1937 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 2160 2216 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 1719 1775 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 1719 1775 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 1506 1562 NO: 61)
[0959] Segment cluster Z21368_PEA.sub.--1_node.sub.--47 (SEQ ID
NO:90) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 35 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00223 TABLE 35 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 2467 2563 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 2389 2485 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 2379 2475 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 2379 2475 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2166 2262 NO: 61)
[0960] Segment cluster Z21368_PEA.sub.--1_node.sub.--49 (SEQ ID NO
91) according to the present invention is supported by 57
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 36 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00224 TABLE 36 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 2564 2658 ID NO: 55) Z21368_PEA 1_T11
(SEQ 2486 2580 ID NO: 56) Z21368_PEA.sub.--1_T5 (SEQ ID 2476 2570
NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID 2476 2570 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2263 2357 NO: 61)
[0961] Segment cluster Z21368_PEA.sub.--1_node.sub.--51 (SEQ ID
NO:92) according to the present invention is supported by 46
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 37 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00225 TABLE 37 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 2659 2724 ID NO: 55) Z21368_PEA 1_T11
(SEQ 2581 2646 ID NO: 56) Z21368_PEA.sub.--1_T5 (SEQ ID 2571 2636
NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID 2571 2636 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2358 2423 NO: 61)
[0962] Segment cluster Z21368_PEA.sub.--1_node.sub.--61 (SEQ ID
NO:93) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 38 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00226 TABLE 38 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 3168 3201 ID NO: 55) Z21368_PEA 1_T11
(SEQ 3090 3123 ID NO: 56) Z21368_PEA.sub.--1_T5 (SEQ ID 2937 2970
NO: 59) Z21368_PEA.sub.--1_T6 (SEQ ID 2937 2970 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 2867 2900 NO: 61)
[0963] Segment cluster Z21368_PEA.sub.--1_node.sub.--68 (SEQ ID NO
94) according to the present invention is supported by 87
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T5 (SEQ
ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID NO:60) and
Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 39 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00227 TABLE 39 Segment location on transcripts Segment
Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 4375 4427 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 4297 4349 ID NO: 56)
Z21368_PEA.sub.--1_T5 (SEQ ID 4144 4196 NO: 59)
Z21368_PEA.sub.--1_T6 (SEQ ID 4144 4196 NO: 60)
Z21368_PEA.sub.--1_T9 (SEQ ID 4074 4126 NO: 61)
[0964] Segment cluster Z21368_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:95) according to the present invention is supported by 29
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z21368_PEA.sub.--1_T10 (SEQ ID NO:55),
Z21368_PEA.sub.--1_T11 (SEQ ID NO:56), Z21368_PEA.sub.--1_T23 (SEQ
ID NO:57), Z21368_PEA.sub.--1_T24 (SEQ ID NO:58),
Z21368_PEA.sub.--1_T5 (SEQ ID NO:59), Z21368_PEA.sub.--1_T6 (SEQ ID
NO:60) and Z21368_PEA.sub.--1_T9 (SEQ ID NO:61). Table 40 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00228 TABLE 40 Segment location on transcripts
Segment Segment Transcript name starting position ending position
Z21368_PEA.sub.--1_T10 (SEQ 463 557 ID NO: 55)
Z21368_PEA.sub.--1_T11 (SEQ 463 557 ID NO: 56)
Z21368_PEA.sub.--1_T23 (SEQ 463 557 ID NO: 57)
Z21368_PEA.sub.--1_T24 (SEQ 463 557 ID NO: 58)
Z21368_PEA.sub.--1_T5 (SEQ ID 301 395 NO: 59) Z21368_PEA.sub.--1_T6
(SEQ ID 301 395 NO: 60) Z21368_PEA.sub.--1_T9 (SEQ ID 328 422 NO:
61)
[0965] Overexpression of at least a portion of this cluster was
determined according to oligonucleotides and one or more chips. The
results were as follows: Oligonucleotide
Z21368.sub.--0.sub.--0.sub.--61857 was on the TM chip and was found
to be overexpressed in breast cancer.
[0966] Variant protein alignment to the previously known
protein:
[0967] Sequence name: /tmp/5ER3vIMKE2/9L0Y7IDITQ:SUL1_HUMAN (SEQ ID
NO:96)
[0968] Sequence Documentation:
[0969] Alignment of: Z21368_PEA.sub.--1_P2 (SEQ ID
NO:97).times.SUL1_HUMAN (SEQ ID NO:96).
[0970] Alignment segment 1/1: TABLE-US-00229 Quality: 7664.00
Score: 0 Matching length: 761 Total length: 761 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00013 Alignment: TABLE-US-00230 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150 . . . 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200 . . . 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250 . . . 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300 . . . 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350 . . . 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400 . . . 401
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 450 . . . 451
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 500 . . . 501
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEF 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEF 550 . . . 551
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 600 . . . 601
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 650 . . . 651
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 700 . . . 701
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 750 . . . 751
NNHWQTAPFWN 761 ||||||||||| 751 NNHWQTAPFWN 761
[0971] Sequence name: /tmp/tt3yfXIUKV/YxSTFWr66h:Q7Z2W2 (SEQ ID
NO:840)
[0972] Sequence Documentation:
[0973] Alignment of: Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98).times.Q7Z2W2 (SEQ ID NO:840).
[0974] Alignment segment 1/1: TABLE-US-00231 Quality: 7869.00
Score: 0 Matching length: 791 Total length: 871 Matching Percent
99.87 Matching Percent Identity: 99.87 Similarity: Total Percent
Similarity: 90.70 Total Percent Identity: 90.70 Gaps: 1
TABLE-US-00014 Alignment: TABLE-US-00232 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELA.......................................... 58 ||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYV 100 . . . 59
......................................FFGKYLNEYNGS 70 ||||||||||||
101 HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTVFFGKYLNEYNGS 150 . . . 71
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 120
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200 . . . 121
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 170
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NYFKMSKRMYPHRPVMMVISHAAPRGPEDSAPQFSKLYPNASQHITPSYN 250 . . . 171
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 220
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300 . . . 221
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 270
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350 . . . 271
PGSIVPQIVLNIDLAPTILDTAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 320
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400 . . . 321
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 370
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 450 . . . 371
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 420
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 500 . . . 421
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEF 470
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQRRSLSVEF 550 . . . 471
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 520
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 600 . . . 521
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 570
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 650 . . . 571
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 620
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 700 . . . 621
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 670
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 750 . . . 671
NNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEY 720
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
NNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEY 800 . . . 721
FDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDV 770
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
FDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDV 850 . . . 771
GNKDGGSYDLHRGQLWDGWEG 791 ||||||||||||||||||||| 851
GNKDGGSYDLHRGQLWDGWEG 871
[0975] Sequence name: /tmp/tt3yfIUKV/YxSTFWr66h:AAH12997 (SEQ ID
NO:841)
[0976] Sequence Documentation:
[0977] Alignment of: Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98).times.AAH12997 (SEQ ID NO:841).
[0978] Alignment segment 1/1: TABLE-US-00233 Quality: 420.00 Score:
0 Matching length: 40 Total length: 40 Matching Percent 100.00
Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00015 Alignment: TABLE-US-00234 . . . 752
LRSCQGYKQCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG 791
|||||||||||||||||||||||||||||||||||||||| 1
LRSCQGYKQCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG 40
[0979] Sequence name: /tmp/tt3yfXIUKV/YxSTFWr66h:SUL1_HUMAN (SEQ ID
NO:96)
[0980] Sequence Documentation:
[0981] Alignment of: Z21368_PEA.sub.--1_P5 (SEQ ID
NO:98).times.SUL1_HUMAN (SEQ ID NO:96).
[0982] Alignment segment 1/1: TABLE-US-00235 Quality: 7878.00
Score: 0 Matching length: 791 Total length: 871 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 90.82 Total Percent Identity: 90.82 Gaps: 1
TABLE-US-00016 Alignment: TABLE-US-00236 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVEL........................................... 57 ||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 58
......................................AFFGKYLNEYNGS 70
||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150 . . . 71
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 120
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200 . . . 121
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 170
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250 . . . 171
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 220
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300 . . . 221
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 270
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350 . . . 271
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 320
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400 . . . 321
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 370
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
NKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARY 450 . . . 371
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 420
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
QTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDK 500 . . . 421
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEF 470
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
DKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEF 550 . . . 471
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 520
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
EGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLA 600 . . . 521
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 570
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
DSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEI 650 . . . 571
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 620
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
EALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLH 700 . . . 621
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 670
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
PFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHD 750 . . . 671
NNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEY 720
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
NNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEY 800 . . . 721
FDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDV 770
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
FDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDV 850 . . . 771
GNKDGGSYDLHRGQLWDGWEG 791 ||||||||||||||||||||| 851
GNKDGGSYDLHRGQLWDGWEG 871
[0983] Sequence name: /tmp/AVAZGWHuF0/RzHFOnHIsT:SUL1_HUMAN (SEQ ID
NO:96)
[0984] Sequence Documentation:
[0985] Alignment of: Z21368_PEA.sub.--1_P15 (SEQ ID
NO:99).times.SUL1_HUMAN (SEQ ID NO:96).
[0986] Alignment segment 1/1: TABLE-US-00237 Quality: 4174.00
Escore: 0 Matching length: 416 Total length: 416 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00017 Alignment: TABLE-US-00238 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150 . . . 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200 . . . 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250 . . . 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300 . . . 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350 . . . 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRT 400 . . . 401
NKKAKIWRDTFLVERG 416 |||||||||||||||| 401 NKKAKIWRDTFLVERG 416
[0987] Sequence name: /tmp/JhwgRdKqmt/kqSmjxkWWk:SUL1_HUMAN (SEQ ID
NO:96)
[0988] Sequence Documentation:
[0989] Alignment of: Z21368_PEA.sub.--1_P16 (SEQ ID
NO:100).times.SUL1_HUMAN (SEQ ID NO:96).
[0990] Alignment segment 1/1: TABLE-US-00239 Quality: 3985.00
Escore: 0 Matching length: 397 Total length: 397 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00018 Alignment: TABLE-US-00240 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150 . . . 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESI 200 . . . 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYN 250 . . . 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNML 300 . . . 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVE 350 . . . 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNR 397
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
PGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNR 397
[0991] Sequence name: /tmp/GPInIw3BOg/zXFdxqG4ow:SUL1_HUMAN (SEQ ID
NO:96)
[0992] Sequence Documentation:
[0993] Alignment of: Z21368_PEA.sub.--1_P22 (SEQ ID
NO:101).times.SUL1_HUMAN (SEQ ID NO:96).
[0994] Alignment segment 1/1: TABLE-US-00241 Quality: 1897.00
Escore: 0 Matching length: 188 Total length: 188 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00019 Alignment: TABLE-US-00242 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGS 150 . . . 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAK 188
|||||||||||||||||||||||||||||||||||||| 151
YIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAK 188
[0995] Sequence name: /tmp/oji5Fs74fB/8xeB9KrGjp:Q7Z2W2 (SEQ ID
NO:840)
[0996] Sequence Documentation:
[0997] Alignment of: Z21368_PEA.sub.--1_P23 (SEQ ID
NO:102).times.Q7Z2W2 (SEQ ID NO:840).
[0998] Alignment segment 1/1: TABLE-US-00243 Quality: 1368.00
Escore: 0.000511 Matching length: 137 Total length: 137 Matching
Percent 100.00 Matching Percent 100.00 Similarity: Identity: Total
Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps:
0
TABLE-US-00020 Alignment: TABLE-US-00244 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRT 137
||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRT 137
[0999] Sequence name: /tmp/oji5Fs74fB/8xeB9KrGjp:SUL1_HUMAN (SEQ ID
NO:96)
[1000] Sequence Documentation:
[1001] Alignment of: Z21368_PEA.sub.--1_P23 (SEQ ID
NO:102).times.SUL1_HUMAN (SEQ ID NO:96).
[1002] Alignment segment 1/1: TABLE-US-00245 Quality: 1368.00
Escore: 0.000511 Matching length: 137 Total length: 137 Matching
Percent 100.00 Matching Percent 100.00 Similarity: Identity: Total
Percent Similarity: 100.00 Total Percent Identity: 100.00 Gaps:
0
TABLE-US-00021 Alignment: TABLE-US-00246 . . . 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLT 50 . . . 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
DDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYV 100 . . . 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRT 137
||||||||||||||||||||||||||||||||||||| 101
HNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRT 137
Expression of SUL1_HUMAN--Extracellular Sulfatase Sulf-1Z21368
Transcripts Which are Detectable by Amplicon as Depicted in
Sequence Name Z21368seg39 (SEQ ID NO:844) in Normal and Cancerous
Breast Tissues
[1003] Expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1
transcripts detectable by or according to seg39, Z21368seg39 (SEQ
ID NO:844) amplicon and Z21368seg39F (SEQ ID NO:842) and
Z21368seg39R (SEQ ID NO:843) primers was measured by real time PCR.
In parallel the expression of four housekeeping genes--PBGD
(GenBank Accession No. BC01 9323 (SEQ ID NO:926),
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)), and G6PD
(GenBank Accession No. NM.sub.--000402 (SEQ ID NO:918);
G6PD-amplicon (SEQ ID NO:921)) was measured similarly. For each RT
sample, the expression of the above amplicon was normalized to the
geometric mean of the quantities of the housekeeping genes. The
normalized quantity of each RT sample was then divided by the
median of the quantities of the normal post-mortem (PM) samples
(Sample Nos. 56-60, 63-67, Table 1 above, Tissue samples in testing
panel), to obtain a value of fold up-regulation for each sample
relative to median of the normal PM samples.
[1004] FIG. 13 is a histogram showing over expression of the
above-indicated SUL1_HUMAN--Extracellular sulfatase Sulf-1
transcripts in cancerous breast samples relative to the normal
samples. Values represent the average of duplicate experiments.
Error bars indicate the minimal and maximal values obtained. The
number and percentage of samples that exhibit at least 5-fold
over-expression, out of the total number of samples tested is
indicated in the bottom.
[1005] As is evident from FIG. 13, the expression of
SUL1_HUMAN--Extracellular sulfatase Sulf-1 transcripts detectable
by the above amplicon(s) in cancer samples was significantly higher
than in the non-cancerous samples (Sample Nos 56-60, 63-67, Table 1
above, Tissue samples in testing panel). Notably an over-expression
of at least 5 fold was found in 13 out of 28 adenocarcinoma
samples.
[1006] Statistical analysis was applied to verify the significance
of these results, as described below.
[1007] The P value for the difference in the expression levels of
SUL1_HUMAN--Extracellular sulfatase Sulf-1 transcripts detectable
by the above amplicon(s) in breast cancer samples versus the normal
tissue samples was determined by T test as 2.14E-03.
[1008] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
6.91E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1009] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: Z21368seg39F
forward primer (SEQ ID NO:842); Z21368seg39R reverse primer (SEQ ID
NO:843).
[1010] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: Z21368seg39 (SEQ ID NO:844). TABLE-US-00247 Z21368seg39F
(SEQ ID NO: 842)-GTTGCATTTCTCAGTGCTGGTTT Z21368seg39R (SEQ ID NO:
843)-AGGGTGCCGGGTGAGG Z21368seg39 (SEQ ID NO:
844)-GTTGCATTTCTCAGTGCTGGTTTCTAATCAGACCAGTGGATTGAGTTTCTCTACCATC
CTCCCCACGTTCTTCTCTAAGCTGCCTCCAAGCCTCACCCGGCACCCT
Expression of SUL1_HUMAN--Extracellular Sulfatase Sulf-1Z21368
Transcripts Which are Detectable by Amplicon as Depicted in
Sequence Name Z21368seg39 (SEQ ID NO:844) in Different Normal
Tissues
[1011] Expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1
transcripts detectable by or according to Z21368seg39 (SEQ ID
NO:844) amplicon and Z21368seg39F (SEQ ID NO:842) Z21368seg39R (SEQ
ID NO:843) was measured by real time PCR. In parallel the
expression of four housekeeping genes--[RPL19 (GenBank Accession
No. NM.sub.--000981 (SEQ ID NO:934); RPL19 amplicon (SEQ ID
NO:937)), TATA box (GenBank Accession No. NM.sub.--003194 (SEQ ID
NO:938); TATA amplicon (SEQ ID NO:941)), UBC (GenBank Accession No.
BC000449 (SEQ ID NO:942); amplicon--Ubiquitin-amplicon (SEQ ID
NO:945)) and SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured
similarly. For each RT sample, the expression of the above amplicon
was normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the breast samples
(sample nos. 33-35 in table 2 "Tissue samples in normal panel") to
obtain a value of relative expression of each sample relative to
median of the Normal samples. Primers and amplicon are as
above.
[1012] The results are presented in FIG. 14, demonstrating the
expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1Z21368
transcripts, which are detectable by amplicon as depicted in
sequence name Z21368seg39 (SEQ ID NO:844), in different normal
tissues.
Expression of SUL1_HUMAN--Extracellular Sulfatase Sulf-1Z21368
Transcripts Which are Detectable by Amplicon as Depicted in
Sequence Name Z21368junc17-21 (SEQ ID NO:847) in Normal and
Cancerous Breast Tissues
[1013] Expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1
transcripts detectable by or according to Z21368junc17-21 (SEQ ID
NO:847) amplicon and Z21368junc17-21F (SEQ ID NO:845) and
Z21368junc17-21R (SEQ ID NO:846) primers was measured by real time
PCR. In parallel the expression of four housekeeping genes--PBGD
(GenBank Accession No. BC019323 (SEQ ID NO:926);
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), and SDHA (GenBank Accession No. NM.sub.--004168 (SEQ
ID NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)), G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos
56-60, 63-67 Table 1 above, "Tissue samples in testing panel"), to
obtain a value of fold up-regulation for each sample relative to
median of the normal PM samples.
[1014] FIG. 15 is a histogram showing over expression of the
above-indicated SUL1_HUMAN--Extracellular sulfatase Sulf-1
transcripts in cancerous breast samples relative to the normal
samples. Values represent the average of duplicate experiments.
Error bars indicate the minimal and maximal values obtained. The
number and percentage of samples that exhibit at least 5 fold
over-expression, out of the total number of samples tested is
indicated in the bottom.
[1015] As is evident from FIG. 15, the expression of
SUL1_HUMAN--Extracellular sulfatase Sulf-1 transcripts detectable
by the above amplicon(s) in cancer samples was significantly higher
than in the non-cancerous samples (Sample Nos 56-60, 63-67, Table 1
above, Tissue samples in testing panel). Notably an over-expression
of at least 5 fold was found in 11 out of 28 adenocarcinoma
samples.
[1016] Statistical analysis was applied to verify the significance
of these results, as described below.
[1017] The P value for the difference in the expression levels of
SUL1_HUMAN--Extracellular sulfatase Sulf-1 transcripts detectable
by the above amplicon(s) in breast cancer samples versus the normal
tissue samples was determined by T test as 4.6E-03.
[1018] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
1.78E-02 as checked by exact fisher test. The above values
demonstrate statistical significance of the results. Primer pairs
are also optionally and preferably encompassed within the present
invention; for example, for the above experiment, the following
primer pair was used as a non-limiting illustrative example only of
a suitable primer pair: Z21368junc17-21F forward primer (SEQ ID
NO:845); Z21368junc17-21R reverse primer (SEQ ID NO:846).
[1019] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: Z21368junc17-21 (SEQ ID NO:847) TABLE-US-00248
Z21368junc17-21F (SEQ ID NO: 845)-GGACGGATACAGCAGGAACG
Z21368junc17-21R (SEQ ID NO: 846)-TATTTTCCAAAAAAGGCCAGCTC
Z21368junc17-21 (SEQ ID NO:
847)-GGACGGATACAGCAGGAACGAAAAAACATCCGACCCAACATTATTCTTGTGCTTAC
CGATGATCAAGATGTGGAGCTGGCCTTTTTTGGAAAATA
Expression of SUL1_HUMAN--Extracellular Sulfatase Sulf-1 Z21368
Transcripts Which are Detectable by Amplicon as Depicted in
Sequence Name Z21368junc17-21 (SEQ ID NO:847) in Different Normal
Tissues
[1020] Expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1
Z21368 transcripts detectable by or according to amplicon
Z21368junc17-21 (SEQ ID NO:847) was measured by real time PCR. In
parallel the expression of four housekeeping genes--RPL19 (GenBank
Accession No. NM.sub.--000981 (SEQ ID NO:934); RPL19 amplicon (SEQ
ID NO:937)), TATA box (GenBank Accession No. NM.sub.--003194 (SEQ
ID NO:938); TATA amplicon (SEQ ID NO:941)), UBC (GenBank Accession
No. BC000449 (SEQ ID NO:942); amplicon--Ubiquitin-amplicon (SEQ ID
NO:945)) and SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured
similarly. For each RT sample, the expression of the above amplicon
was normalized to the geometric mean of the quantities of the
housekeeping genes, as above. The normalized quantity of each RT
sample was then divided by the median of the quantities of the
breast samples (Sample Nos. --33-35 Table 2 above, "Tissue samples
on normal panel"), to obtain a value of relative expression of each
sample relative to median of the breast samples. Primers and
amplicon are as above.
[1021] The results are presented in FIG. 16, demonstrating the
expression of SUL1_HUMAN--Extracellular sulfatase Sulf-1 Z21368
transcripts, which are detectable by amplicon as depicted in
sequence name Z21368junc17-21 (SEQ ID NO:847), in different normal
tissues.
Description for Cluster T59832
[1022] Cluster T59832 features 6 transcript(s) and 33 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
5 application. The selected protein variants are given in table 3.
TABLE-US-00249 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No T59832_T11 103 T59832_T15 104 T59832_T22 105
T59832_T28 106 T59832_T6 107 T59832_T8 108
[1023] TABLE-US-00250 TABLE 2 Segments of interest Segment Name
Sequence ID No T59832_node.sub.--1 109 T59832_node.sub.--22 110
T59832_node.sub.--23 111 T59832_node.sub.--24 112
T59832_node.sub.--29 113 T59832_node.sub.--39 114
T59832_node.sub.--7 115 T59832_node.sub.--10 116
T59832_node.sub.--11 117 T59832_node.sub.--12 118
T59832_node.sub.--14 119 T59832_node.sub.--16 120
T59832_node.sub.--19 121 T59832_node.sub.--2 122
T59832_node.sub.--20 123 T59832_node.sub.--25 124
T59832_node.sub.--26 125 T59832_node.sub.--27 126
T59832_node.sub.--28 127 T59832_node.sub.--3 128
T59832_node.sub.--30 129T59832_node.sub.--31
130T59832_node.sub.--32 131 T59832_node.sub.--34 132
T59832_node.sub.--35 133 T59832_node.sub.--36 134
T59832_node.sub.--37 135 T59832_node.sub.--38 136
T59832_node.sub.--4 137T59832_node.sub.--5 138T59832_node.sub.--6
139T59832_node.sub.--8 140 T59832_node.sub.--9 141
[1024] TABLE-US-00251 TABLE 3 Proteins of interest Protein Name
Sequence ID No T59832_P5 143 T59832_P7 144 T59832_P9 145 T59832_P12
146 T59832_P18 147
[1025] These sequences are variants of the known protein
Gamma-interferon inducible lysosomal thiol reductase precursor
(SwissProt accession identifier GILT_HUMAN; known also according to
the synonyms Gamma-interferon-inducible protein IP-30), SEQ ID NO:
142, referred to herein as the previously known protein.
[1026] Protein Gamma-interferon inducible lysosomal thiol reductase
precursor (SEQ ID NO:142) is known or believed to have the
following function(s): Cleaves disulfide bonds in proteins by
reduction. May facilitate the complete unfolding of proteins
destined for lysosomal degradation. May be involved in MHC class
II-restricted antigen processing. The sequence for protein
Gamma-interferon inducible lysosomal thiol reductase precursor (SEQ
ID NO:142) is given at the end of the application, as
"Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142) amino acid sequence". Known polymorphisms for this
sequence are as shown in Table 4. TABLE-US-00252 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 109 L->S 130H->L
[1027] Protein Gamma-interferon inducible lysosomal thiol reductase
precursor (SEQ ID NO:142) localization is believed to be
Lysosomal.
[1028] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: extracellular;
lysosome, which are annotation(s) related to Cellular
Component.
[1029] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1030] Cluster T59832 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 17 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1031] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 17 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: brain malignant tumors, breast malignant
tumors, ovarian carcinoma and pancreas carcinoma. TABLE-US-00253
TABLE 5 Normal tissue distribution Name of Tissue Number Adrenal
208 Bladder 205 Bone 200 Brain 18 Colon 236 Epithelial 143 General
280 head and neck 192 Kidney 71 Liver 53 Lung 459 lymph nodes 248
Breast 0 bone marrow 94 Ovary 0 Pancreas 20 Prostate 86 Skin 29
Stomach 109 T cells 557 Thyroid 0 Uterus 63
[1032] TABLE-US-00254 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 4.9e-01
5.9e-01 4.7e-03 1.1 2.9e-02 0.8 bladder 3.7e-01 5.6e-01 3.7e-02 1.3
2.5e-01 0.9 Bone 6.6e-01 6.7e-01 3.4e-01 0.6 9.1e-01 0.4 Brain
1.8e-01 2.9e-01 4.3e-03 3.8 2.8e-02 2.5 colon 4.4e-01 5.2e-01 6.1
e-01 0.9 8.1e-01 0.7 epithelial 2.5e-02 1.6e-01 1.2e-05 1.6 9.8e-02
1.1 general 1.3e-02 1.6e-01 1 0.8 1 0.6 Head and neck 3.4e-01
3.3e-01 1 0.4 9.4e-01 0.5 kidney 7.7e-01 8.5e-01 1.4e-01 1.3
4.2e-01 0.9 Liver 8.3e-01 7.6e-01 1 0.5 1 0.6 Lung 5.7e-01 8.3e-01
3.5e-01 0.8 9.8e-01 0.5 lymph nodes 5.7e-01 6.6e-01 7.6e-01 0.8
3.6e-02 1.1 breast 5.0e-02 1.3e-01 2.5e-03 6.5 4.4e-02 3.6 Bone
marrow 6.2e-01 7.8e-01 1 0.3 9.5e-01 0.5 ovary 2.2e-01 9.4e-02
3.2e-03 6.1 8.3e-03 5.3 pancreas 9.0e-02 1.6e-02 1.1e-03 4.0
7.9e-04 4.2 prostate 8.1e-01 8.0e-01 5.7e-01 0.9 4.1e-01 0.9 skin
1.6e-01 1.2e-01 2.3e-02 6.0 1.0e-02 2.2 stomach 5.5e-01 7.4e-01
9.4e-01 0.6 4.9e-01 1.0 T cells 1 6.7e-01 6.9e-01 1.0 9.8e-01 0.5
Thyroid 2.3e-01 2.3e-01 5.9e-02 2.5 5.9e-02 2.5 uterus 7.4e-02
4.7e-02 2.2e-02 2.0 6.2e-02 1.7
[1033] As noted above, cluster T59832 features 6 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Gamma-interferon
inducible lysosomal thiol reductase precursor (SEQ ID NO:142). A
description of each variant protein according to the present
invention is now provided.
[1034] Variant protein T59832_P5 (SEQ ID NO:143) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T59832_T6 (SEQ ID
NO:107). An alignment is given to the known protein
(Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application.
[1035] Comparison report between T59832_P5 (SEQ ID NO:143) and
GILT_HUMAN (SEQ ID NO: 142):
[1036] 1. An isolated chimeric polypeptide encoding for T59832_P5
(SEQ ID NO:143), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 12-55 of GILT_HUMAN (SEQ ID NO:142), which also corresponds
to amino acids 1-44 of T59832_P5 (SEQ ID NO:143), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP
SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSMGQGPPACIL
AATLDAFIPARAGLACLWDLLGRCPRG (SEQ ID NO:1010) corresponding to amino
acids 45-189 of T59832_P5 (SEQ ID NO:143), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1037] 2. An isolated polypeptide encoding for a tail of T59832_P5
(SEQ ID NO: 143), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence TABLE-US-00255
VGTATGRAGWREQAPCRGTRLLLSPQTSQGKTRAPRGRCPCRVPGKTLFSSRRCGHTP (SEQ ID
NO: 1010)
SVPFRFRIPHLRGAAASTRLVPPKGSMSAYCVLLGQELGSPFVAQGTSSAAGQGPPACIL
AATLDAFIPARAGLACLWDLLGRCPRG in T59832_P5. (SEQ ID NO: 143)
[1038] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1039] Variant protein T59832_P5 (SEQ ID NO:143) is encoded by the
following transcript(s): T59832_T6 (SEQ ID NO:107), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T59832_T6 (SEQ ID NO:107) is shown in bold;
this coding portion starts at position 149 and ends at position
715. The transcript also has the following SNPs as listed in Table
7 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T59832_P5 (SEQ ID NO:143) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00256 TABLE 7 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 61 C->T Yes 148 G->T Yes 1505 G->C Yes 1651
T->No 1652 T->G Yes 1717 C->A No 1722 C->No 1722
C->G No 1752A->G Yes 1817 A->G Yes 1854 C->No 1854
C->A No 212->A No 1871 C->T Yes 1886 T->G No 1906
G->A No 1906 G->C No 1942 C->No 1942 C->T No 1971
C->No 1986 G->A No 2001 G->T Yes 2008 A->No 241 G->T
No 2030->T No 2031 C->T No 2050 C->No 2056 A->G Yes
2068 G->A Yes 2111 A->C Yes 2136 A->C Yes 2144 T->C Yes
244 A->G Yes 962 C->T Yes 1074 G->A Yes 1248 G->C Yes
1441 G->A Yes 1443 G->A No
[1040] Variant protein T59832_P7 (SEQ ID NO:144) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T59832_T8 (SEQ ID
NO:108). An alignment is given to the known protein
(Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1041] Comparison report between T59832_P7 (SEQ ID NO:144) and
GILT_HUMAN (SEQ ID NO:142):
[1042] 1. An isolated chimeric polypeptide encoding for T59832_P7
(SEQ ID NO: 144), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
12-223 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding
to amino acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1043] 2. An isolated polypeptide encoding for a tail of T59832_P7
(SEQ ID NO:144), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID
NO:1011) in T59832_P7 (SEQ ID NO:144).
[1044] Comparison report between T59832_P7 (SEQ ID NO:144) and
BAC98466 (SEQ ID NO:848):
[1045] 1. An isolated chimeric polypeptide encoding for T59832_P7
(SEQ ID NO:144), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
1-212 of BAC98466 (SEQ ID NO:848), which also corresponds to amino
acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding to amino
acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1046] 2. An isolated polypeptide encoding for a tail of T59832_P7
(SEQ ID NO:144), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID
NO:1011) in T59832_P7 (SEQ ID NO:144).
[1047] Comparison report between T59832_P7 (SEQ ID NO:144) and
BAC85622 (SEQ ID NO:849):
[1048] 1. An isolated chimeric polypeptide encoding for T59832_P7
(SEQ ID NO:144), comprising a first amino acid sequence being at
least 70%, optionally at least 80%, preferably at least 85%, more
preferably at least 90% and most preferably at least 95% homologous
to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P7 (SEQ ID NO:144), and a second amino
acid sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC
MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYV
PWVTVNGVRIFLALSLTLIVPWSQGWTRQRDQR corresponding to amino acids
1-148 of BAC85622 (SEQ ID NO:849), which also corresponds to amino
acids 91-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1049] 2. An isolated polypeptide encoding for a head of T59832_P7
(SEQ ID NO:144), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence TABLE-US-00257
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832_P7. (SEQ ID NO:
144)
[1050] Comparison report between T59832_P7 (SEQ ID NO:144) and
Q8WU77 (SEQ ID NO:850):
[1051] 1. An isolated chimeric polypeptide encoding for T59832_P7
(SEQ ID NO:144), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding to amino acids
1-212 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 1-212 of T59832_P7 (SEQ ID NO:144), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID NO:1011) corresponding to amino
acids 213-238 of T59832_P7 (SEQ ID NO:144), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1052] 2. An isolated polypeptide encoding for a tail of T59832_P7
(SEQ ID NO:144), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence VRIFLALSLTLIVPWSQGWTRQRDQR (SEQ ID
NO:1011) in T59832_P7 (SEQ ID NO:144).
[1053] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide.
[1054] Variant protein T59832_P7 (SEQ ID NO:144) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T59832_P7 (SEQ ID NO:144) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00258 TABLE 8 Amino
acid mutations SNP position(s) on Alternative Previously amino acid
sequence amino acid(s) known SNP? 146 I->No 146 I->M Yes 168
P->Q No 170 L->No 170 L->V No 180 M->V Yes 76 R->Q
Yes 77 A->T No
[1055] Variant protein T59832_P7 (SEQ ID NO:144) is encoded by the
following transcript(s): T59832_T8 (SEQ ID NO:108), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T59832_T8 (SEQ ID NO:108) is shown in bold;
this coding portion starts at position 149 and ends at position
862. The transcript also has the following SNPs as listed in Table
9 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T59832_P7 (SEQ ID NO:144) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00259 TABLE 9 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 61 C->T Yes 148 G->T Yes 651 C->A No 656
C->No 656 C->G No 686 A->G Yes 751 A->G Yes 1004
T->G Yes 1206 C->No 1206 C->A No 1223 C->T Yes 1238
T->G No 212->A No 1258 G->A No 1258 G->C No 1294
C->No 1294 C->T No 1323 C->No 1338 G->A No 1353 G->T
Yes 1360A->No 1382->T No 1383 C->T No 241 G->T No 1402
C->No 1408 A->G Yes 1420 G->A Yes 1463 A->C Yes 1488
A->C Yes 1496 T->C Yes 244 A->G Yes 375 G->A Yes 377
G->A No 439 G->C Yes 585 T->No 586 T->G Yes
[1056] Variant protein T59832_P9 (SEQ ID NO:145) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T59832_T11 (SEQ ID
NO:103). An alignment is given to the known protein
(Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1057] Comparison report between T59832_P9 (SEQ ID NO:145) and
GILT_HUMAN (SEQ ID NO:2):
[1058] 1. An isolated chimeric polypeptide encoding for T59832_P9
(SEQ ID NO:145), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 12-214 of
GILT_HUMAN (SEQ ID NO:142), which also corresponds to amino acids
1-203 of T59832_P9 (SEQ ID NO:145), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1013)
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1059] 2. An isolated polypeptide encoding for a tail of T59832_P9
(SEQ ID NO:145), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[1060] Comparison report between-T59832_P9 (SEQ ID NO:145) and
BAC98466 (SEQ ID NO:848):
[1061] 1. An isolated chimeric polypeptide encoding for T59832_P9
(SEQ ID NO:145), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of
BAC98466 (SEQ ID NO:848), which also corresponds to amino acids
1-203 of T59832_P9 (SEQ ID NO:145), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO: 1013)
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1062] 2. An isolated polypeptide encoding for a tail of T59832_P9
(SEQ ID NO: 145), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[1063] Comparison report between T59832_P9 (SEQ ID NO:145) and
BAC85622 (SEQ ID NO:849):
[1064] 1. An isolated chimeric polypeptide encoding forT59832_P9
(SEQ ID NO:145), comprising a first amino acid sequence being at
least 70%, optionally at least 80%, preferably at least 85%, more
preferably at least 90% and most preferably at least 95% homologous
to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P9 (SEQ ID NO:145), second amino acid
sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVC
MEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHE
corresponding to amino acids 1-113 of BAC85622 (SEQ ID NO:849),
which also corresponds to amino acids 91-203 of T59832_P9 (SEQ ID
NO:145), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013)
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first, second and third amino acid sequences are
contiguous and in a sequential order.
[1065] 2. An isolated polypeptide encoding for a head of T59832_P9
(SEQ ID NO:145), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence TABLE-US-00260
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832_P9. (SEQ ID NO:
145)
[1066] 3. An isolated polypeptide encoding for a tail of T59832_P9
(SEQ ID NO:145), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[1067] Comparison report between T59832_P9 (SEQ ID NO:145) and
Q8WU77 (SEQ ID NO:850):
[1068] 1. An isolated chimeric polypeptide encoding for T59832_P9
(SEQ ID NO:145), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIM
ECAMGDRGMQLMHANAQRTDALQPPHE corresponding to amino acids 1-203 of
Q8WU77 (SEQ ID NO:850), which also corresponds to amino acids 1-203
of T59832 P9 (SEQ ID NO:145), and a second amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence TABLE-US-00261 (SEQ
ID NO: 1013) NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR
corresponding to amino acids 204-244 of T59832_P9 (SEQ ID NO:145),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1069] 2. An isolated polypeptide encoding for a tail of T59832_P9
(SEQ ID NO:145), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence
NPWKIRPSSLPLSASCTRARSRMSALPQPAPSGVFASSDGR (SEQ ID NO:1013) in
T59832_P9 (SEQ ID NO:145).
[1070] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1071] Variant protein T59832_P9 (SEQ ID NO:145) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 10, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T59832_P9 SEQ ID NO:145) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00262 TABLE 10 Amino
acid mutations SNP position(s) on Alternative Previously amino acid
sequence amino acid(s) known SNP? 146 I->No 146 I->M Yes 222
A->P No 222 A->T No 234 P->No 234 P->S No 243 G->No
76 R->Q Yes 77 A->T No 168 P->Q No 170 L->No 170
L->V No 180 M->V Yes 204 N->No 204 N->K No 210 P->L
Yes 215 L->W No
[1072] Variant protein T59832_P9 (SEQ ID NO:145) is encoded by the
following transcript(s): T59832_T11 (SEQ ID NO:103), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T59832_T11 (SEQ ID NO:103) is shown in bold;
this coding portion starts at position 149 and ends at position
880. The transcript also has the following SNPs as listed in Table
11 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T59832_P9 (SEQ ID NO:145) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00263 TABLE 11 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 61 C->T Yes 148 G->T Yes 651 C->A No 656
C->No 656 C->G No 686 A->G Yes 751 A->G Yes 760
C->No 760 C->A No 777 C->T Yes 792 T->G No 812 G->A
No 212->A No 812 G->C No 848 C->No 848 C->T No 877
C->No 892 G->A No 907 G->T Yes 914 A->No 936->T No
937 C->T No 956 C->No 241 G->T No 962 A->G Yes 974
G->A Yes 1017A->C Yes 1042 A->C Yes 1050 T->C Yes 244
A->G Yes 375 G->A Yes 377 G->A No 439 G->C Yes 585
T->No 586 T->G Yes
[1073] Variant protein T59832_P12 (SEQ ID NO:146) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T59832_T15 (SEQ ID
NO:104). An alignment is given to the known protein
(Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1074] Comparison report between T59832_P12 (SEQ ID NO:146) and
GILT_HUMAN (SEQ ID NO: 142):
[1075] 1. An isolated chimeric polypeptide encoding for T59832_P12
(SEQ ID NO:146), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVE corresponding to amino acids 12-141 of GILT_HUMAN
(SEQ ID NO:142), which also corresponds to amino acids 1-130 of
T59832_P12 (SEQ ID NO:146), and a second amino acid sequence being
at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
173-261 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 131-219 of T59832_P12 (SEQ ID NO:146), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1076] 2. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P12 (SEQ ID NO:146), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise EC, having a structure as follows: a sequence
starting from any of amino acid numbers 130-x to 130; and ending at
any of amino acid numbers 131+((n-2)-x), in which x varies from 0
to n-2.
[1077] Comparison report between T59832_P12 (SEQ ID NO:146) and
BAC85622 (SEQ ID NO:849):
[1078] 1. An isolated chimeric polypeptide encoding for T59832_P12
(SEQ ID NO:146), comprising a first amino acid sequence being at
least 70%, optionally at least 80%, preferably at least 85%, more
preferably at least 90% and most preferably at least 95% homologous
to a polypeptide having the sequence
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLV (SEQ ID NO:1012) corresponding to
amino acids 1-90 of T59832_P12 (SEQ ID NO:146), second amino acid
sequence being at least 90% homologous to
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVE corresponding to amino
acids 1-40 of BAC85622 (SEQ ID NO:849), which also corresponds to
amino acids 91-130 of T59832_P12 (SEQ ID NO:146), third amino acid
sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNG corresponding
to amino acids 72-122 of BAC85622 (SEQ ID NO:849), which also
corresponds to amino acids 131-181 of T59832_P12 (SEQ ID NO:146),
and a fourth amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID
NO:1016) corresponding to amino acids 182-219 of T59832_P12 (SEQ ID
NO:146), wherein said first, second, third and fourth amino acid
sequences are contiguous and in a sequential order.
[1079] 2. An isolated polypeptide encoding for a head of T59832_P12
(SEQ ID NO:146), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence TABLE-US-00264
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA (SEQ ID
NO: 1012) PLVNVTLYYEALCGGCRAFLIRELFPTWLLV of T59832_P12. (SEQ ID
NO: 146)
[1080] 3. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P12 (SEQ ID NO:146), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise EC, having a structure as follows: a sequence
starting from any of amino acid numbers 130-x to 130; and ending at
any of amino acid numbers 131+((n-2)-x), in which x varies from 0
to n-2.
[1081] 4. An isolated polypeptide encoding for a tail of T59832_P12
(SEQ ID NO: 146), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence
KPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK (SEQ ID NO:1016) in
T59832_P12 (SEQ ID NO:146).
[1082] Comparison report between T59832_P12 (SEQ ID NO:146) and
Q8WU77 (SEQ ID NO:850):
[1083] 1. An isolated chimeric polypeptide encoding for T59832_P12
(SEQ ID NO:146), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNA
PLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKC
QHGEEECKFNKVE corresponding to amino acids 1-130 of Q8WU77 (SEQ ID
NO:850), which also corresponds to amino acids 1-130 of T59832_P12
(SEQ ID NO:146), and a second amino acid sequence being at least
90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 131-219 of T59832_P12 (SEQ ID NO:146), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1084] 2. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P12 (SEQ ID NO:146), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise EC, having a structure as follows: a sequence
starting from any of amino acid numbers 130-x to 130; and ending at
any of amino acid numbers 131+((n-2)-x), in which x varies from 0
to n-2.
[1085] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1086] Variant protein T59832_P12 (SEQ ID NO:146) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 12, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T59832_P12 (SEQ ID NO:146)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00265 TABLE
12 Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 137 P->Q No 139
L->No 76 R->Q Yes 77 A->T No 139 L->V No 149 M->V
Yes 183 P->No 183 P->T No 200 G->A No 200 G->D No 212
S->No 212 S->F No
[1087] Variant protein T59832_P12 (SEQ ID NO:146) is encoded by the
following transcript(s): T59832_T15 (SEQ ID NO:104), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T59832_T15 (SEQ ID NO: 104) is shown in bold;
this coding portion starts at position 149 and ends at position
805. The transcript also has the following SNPs as listed in Table
13 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T59832_P12 (SEQ ID NO:146) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00266 TABLE 13 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 61 C->T Yes 148 G->T Yes 563 C->G
No 593 A->G Yes 658 A->G Yes 695 C->No 695 C->A No 712
C->T Yes 727 T->G No 747 G->A No 747 G->C No 783
C->No 212->A No 783 C->T No 812 C->No 827 G->A No
842 G->T Yes 849 A->No 871->T No 872 C->T No 891
C->No 897 A->G Yes 909 G->A Yes 241 G->T No 952 A->C
Yes 977 A->C Yes 985 T->C Yes 244 A->G Yes 375 G->A Yes
377 G->A No 439 G->C Yes 558 C->A No 563 C->No
[1088] Variant protein T59832_P18 (SEQ ID NO:147) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) T59832_T22 (SEQ ID
NO:105). An alignment is given to the known protein
(Gamma-interferon inducible lysosomal thiol reductase precursor
(SEQ ID NO:142)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1089] Comparison report between T59832_P18 (SEQ ID NO:147) and
GILT_HUMAN (SEQ ID NO: 142):
[1090] 1. An isolated chimeric polypeptide encoding for T59832_P18
(SEQ ID NO:147), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 12-55 of GILT_HUMAN (SEQ ID NO:142), which also corresponds
to amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second
amino acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
173-261 of GILT_HUMAN (SEQ ID NO:142), which also corresponds to
amino acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1091] 2. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P18 (SEQ ID NO:147), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise KC, having a structure as follows: a sequence
starting from any of amino acid numbers 44-x to 44; and ending at
any of amino acid numbers 45+((n-2)-x), in which x varies from 0 to
n-2.
[1092] Comparison report between T59832_P18 (SEQ ID NO:147) and
Q8WU77 (SEQ ID NO:850):
[1093] 1. An isolated chimeric polypeptide encoding for T59832_P18
(SEQ ID NO:147), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 1-44 of Q8WU77 (SEQ ID NO:850), which also corresponds to
amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second amino
acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8WU77 (SEQ ID NO:850), which also corresponds to amino
acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1094] 2. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P18 (SEQ ID NO:147), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise KC, having a structure as follows: a sequence
starting from any of amino acid numbers 44-x to 44; and ending at
any of amino acid numbers 45+((n-2)-x), in which x varies from 0 to
n-2.
[1095] Comparison report between T59832_P18 (SEQ ID NO:147) and
Q8NE14 (SEQ ID NO:851):
[1096] 1. An isolated chimeric polypeptide encoding forT59832_P18
(SEQ ID NO:147), comprising a first amino acid sequence being at
least 90% homologous to
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK corresponding to amino
acids 1-44 of Q8NE14 (SEQ ID NO:851), which also corresponds to
amino acids 1-44 of T59832_P18 (SEQ ID NO:147), and a second amino
acid sequence being at least 90% homologous to
CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLED
QTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK corresponding to amino acids
162-250 of Q8NE14 (SEQ ID NO:851), which also corresponds to amino
acids 45-133 of T59832_P18 (SEQ ID NO:147), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[1097] 2. An isolated chimeric polypeptide encoding for an edge
portion of T59832_P18 (SEQ ID NO:147), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise KC, having a structure as follows: a sequence
starting from any of amino acid numbers 44-x to 44; and ending at
any of amino acid numbers 45+((n-2)-x), in which x varies from 0 to
n-2.
[1098] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1099] Variant protein T59832_P18 (SEQ ID NO:147) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 14, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T59832_P18 (SEQ ID NO:147)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00267 TABLE
14 Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 114 G->A No 114
G->D No 126 S->No 126 S->F No 51 P->Q No 53 L->No 53
L->V No63 M->V Yes 97 P->No 97 P->T No
[1100] Variant protein T59832_P18 (SEQ ID NO:147) is encoded by the
following transcript(s): T59832_T22 (SEQ ID NO:105), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript T59832_T22 (SEQ ID NO:105) is shown in bold;
this coding portion starts at position 149 and ends at position
547. The transcript also has the following SNPs as listed in Table
15 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T59832_P18 (SEQ ID NO:147) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00268 TABLE 15 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 61 C->T Yes 148 G->T Yes 437 C->No
437 C->A No 454 C->T Yes 469 T->G No 489 G->A No 489
G->C No 525 C->No 525 C->T No 554 C->No 569 G->A No
212->A No 584 G->T Yes 591 A->No 613->T No 614 C->T
No 633 C->No 639 A->G Yes 651 G->A Yes 694 A->C Yes 719
A->C Yes 727 T->C Yes 241 G->T No 244 A->G Yes 300
C->A No 305 C->No 305 C->G No 335 A->G Yes 400 A->G
Yes
[1101] As noted above, cluster T59832 features 33 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1102] Segment cluster T59832_node.sub.--1 (SEQ ID NO:109)
according to the present invention is supported by 62 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID
NO:108). Table 16 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00269 TABLE 16 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T11 (SEQ ID 1 123 NO: 103)
T59832_T15 (SEQ ID 1 123 NO: 104) T59832_T22 (SEQ ID 1 123 NO: 105)
T59832_T6 (SEQ ID NO: 107) 1 123T59832_T8 (SEQ ID NO: 108) 1
123
[1103] Segment cluster T59832_node.sub.--22 (SEQ ID NO:110)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T59832_T28
(SEQ ID NO: 106). Table 17 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00270 TABLE
17 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T28 (SEQ ID 1 523 NO:
106)
[1104] Segment cluster T59832_node.sub.--23 (SEQ ID NO:111)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T59832_T28
(SEQ ID NO:106). Table 18 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00271 TABLE
18 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T28 (SEQ ID 524 652 NO:
106)
[1105] Segment cluster T59832_node.sub.--24 (SEQ ID NO:112)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T59832_T28
(SEQ ID NO:106). Table 19 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00272 TABLE
19 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T28 (SEQ ID 653 901 NO:
106)
[1106] Segment cluster T59832_node.sub.--29 (SEQ ID NO:113)
according to the present invention is supported by 12 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T28 (SEQ ID NO:106) and T59832_T8 (SEQ ID NO:108). Table 20
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00273 TABLE 20 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T28 (SEQ ID 1055 1472 NO: 106) T59832_T8
(SEQ ID NO: 108) 785 1202
[1107] Segment cluster T59832_node.sub.--39 (SEQ ID NO:114)
according to the present invention is supported by 195 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832_T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 21 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00274 TABLE 21 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 1031 1084 NO: 103) T59832_T15 (SEQ ID 966 1019
NO: 104) T59832_T22 (SEQ ID 708 761 NO: 105) T59832_T28 (SEQ ID
1747 1800 NO: 106) T59832_T6 (SEQ ID NO: 107) 2125 2178 T59832_T8
(SEQ ID NO: 108) 1477 1530
[1108] Segment cluster T59832_node.sub.--7 (SEQ ID NO:115)
according to the present invention is supported by 8 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): T59832_T6 (SEQ
ID NO:107). Table 22 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00275 TABLE
22 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T6 (SEQ ID NO: 107) 281
1346
[1109] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1110] Segment cluster T59832_node.sub.--10 (SEQ ID NO: 116)
according to the present invention is supported by 332 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 23 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00276 TABLE 23 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 338 382 NO: 103) T59832_T15 (SEQ ID 338 382 NO:
104) T59832_T6 (SEQ ID NO: 107) 1404 1448 T59832_T8 (SEQ ID NO:
108) 338 382
[1111] Segment cluster T59832_node.sub.--11 (SEQ ID NO: 117)
according to the present invention is supported by 306 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 24 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00277 TABLE 24 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 383 417 NO: 103) T59832_T15 (SEQ ID 383 417 NO:
104) T59832_T6 (SEQ ID NO: 107) 1449 1483 T59832_T8 (SEQ ID NO:
108) 383 417
[1112] Segment cluster T59832_node.sub.--12 (SEQ ID NO:118)
according to the present invention is supported by 280 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 25 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00278 TABLE 25 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 418 463 NO: 103) T59832_T15 (SEQ ID 418 463 NO:
104) T59832_T6 (SEQ ID NO: 107) 1484 1529 T59832_T8 (SEQ ID NO:
108) 418 463
[1113] Segment cluster T59832_node.sub.--14 (SEQ ID NO: 119)
according to the present invention is supported by 280 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 26 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00279 TABLE 26 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 464 502 NO: 103) T59832_T15 (SEQ ID 464 502 NO:
104) T59832_T6 (SEQ ID NO: 107) 1530 1568 T59832_T8 (SEQ ID NO:
108) 464 502
[1114] Segment cluster T59832_node.sub.--16 (SEQ ID NO:120)
according to the present invention is supported by 287 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 27 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00280 TABLE 27 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 503 538 NO: 103) T59832_T15 (SEQ ID 503 538 NO:
104) T59832_T6 (SEQ ID NO: 107) 1569 1604 T59832_T8 (SEQ ID NO:
108) 503 538
[1115] Segment cluster T59832_node.sub.--19 (SEQ ID NO:121)
according to the present invention is supported by 300 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T6 (SEQ ID NO:107) and T59832_T8
(SEQ ID NO:108). Table 28 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00281 TABLE
28 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T11 (SEQ ID 539 577 NO:
103) T59832_T6 (SEQ ID NO: 107) 1605 1643 T59832_T8 (SEQ ID NO:
108) 539 577
[1116] Segment cluster T59832_node.sub.--2 (SEQ ID NO:122)
according to the present invention is supported by 258 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID
NO:108). Table 29 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00282 TABLE 29 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T11 (SEQ ID 124 154 NO: 103)
T59832_T15 (SEQ ID 124 154 NO: 104) T59832_T22 (SEQ ID 124 154 NO:
105) T59832_T6 (SEQ ID NO: 107) 124 154 T59832_T8 (SEQ ID NO: 108)
124 154
[1117] Segment cluster T59832_node.sub.--20 (SEQ ID NO:123)
according to the present invention is supported by 318 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T6 (SEQ ID NO:107) and T59832_T8
(SEQ ID NO:108). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00283 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position T59832_T11 (SEQ ID 578 631 NO:
103) T59832_T6 (SEQ ID NO: 107) 1644 1697 T59832_T8 (SEQ ID NO:
108) 578 631
[1118] Segment cluster T59832_node.sub.--25 (SEQ ID NO:124)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 31
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00284 TABLE 31 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 632 653 NO: 103) T59832_T15 (SEQ
ID 539 560 NO: 104) T59832_T22 (SEQ ID 281 302 NO: 105) T59832_T28
(SEQ ID 902 923 NO: 106) T59832_T6 (SEQ ID NO: 107) 1698 1719
T59832_T8 (SEQ ID NO: 108) 632 653
[1119] Segment cluster T59832_node.sub.--26 (SEQ ID NO:125)
according to the present invention is supported by 342 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832 T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 32 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00285 TABLE 32 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 654 717 NO: 103) T59832_T15 (SEQ ID 561 624 NO:
104) T59832_T22 (SEQ ID 303 366 NO: 105) T59832_T28 (SEQ ID 924 987
NO: 106) T59832_T6 (SEQ ID NO: 107) 1720 1783 T59832_T8 (SEQ ID NO:
108) 654 717
[1120] Segment cluster T59832_node.sub.--27 (SEQ ID NO: 126)
according to the present invention is supported by 314 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832_T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 33 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00286 TABLE 33 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 718 756 NO: 103) T59832_T15 (SEQ ID 625 663 NO:
104) T59832_T22 (SEQ ID 367 405 NO: 105) T59832_T28 (SEQ ID 988
1026 NO: 106) T59832_T6 (SEQ ID NO: 107) 1784 1822 T59832_T8 (SEQ
ID NO: 108) 718 756
[1121] Segment cluster T59832_node.sub.--28 (SEQ ID NO:127)
according to the present invention is supported by 284 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T15 (SEQ ID NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28
(SEQ ID NO:106), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID
NO:108). Table 34 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00287 TABLE 34 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T15 (SEQ ID 664 691 NO: 104)
T59832_T22 (SEQ ID 406 433 NO: 105) T59832_T28 (SEQ ID 1027 1054
NO: 106) T59832_T6 (SEQ ID NO: 107) 1823 1850 T59832_T8 (SEQ ID NO:
108) 757 784
[1122] Segment cluster T59832_node.sub.--3 (SEQ ID NO:128)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and
T59832_T8 (SEQ ID NO:108). Table 35 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00288 TABLE 35 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 155 172 NO: 103) T59832_T15 (SEQ ID 155 172 NO:
104) T59832_T22 (SEQ ID 155 172 NO: 105) T59832_T6 (SEQ ID NO: 107)
155 172 T59832_T8 (SEQ ID NO: 108) 155 172
[1123] Segment cluster T59832_node.sub.--30 (SEQ ID NO:129)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 36
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00289 TABLE 36 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 757 760 NO: 103) T59832_T15 (SEQ
ID 692 695 NO: 104) T59832_T22 (SEQ ID 434 437 NO: 105) T59832_T28
(SEQ ID 1473 1476 NO: 106) T59832_T6 (SEQ ID NO: 107) 1851 1854
T59832_T8 (SEQ ID NO: 108) 1203 1206
[1124] Segment cluster T59832_node.sub.--31 (SEQ ID NO:130)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 37
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00290 TABLE 37 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 761 780 NO: 103) T59832_T15 (SEQ
ID 696 715 NO: 104) T59832_T22 (SEQ ID 438 457 NO: 105) T59832_T28
(SEQ ID 1477 1496 NO: 106) T59832_T6 (SEQ ID NO: 107) 1855 1874
T59832_T8 (SEQ ID NO: 108) 1207 1226
[1125] Segment cluster T59832_node.sub.--32 (SEQ ID NO:131)
according to the present invention is supported by 287 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832_T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 38 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00291 TABLE 38 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 781 810 NO: 103) T59832_T15 (SEQ ID 716 745 NO:
104) T59832_T22 (SEQ ID 458 487 NO: 105) T59832_T28 (SEQ ID 1497
1526 NO: 106) T59832_T6 (SEQ ID NO: 107) 1875 1904 T59832_T8 (SEQ
ID NO: 108) 1227 1256
[1126] Segment cluster T59832_node.sub.--34 (SEQ ID NO: 132)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 39
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00292 TABLE 39 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 811 832 NO: 103) T59832_T15 (SEQ
ID 746 767 NO: 104) T59832_T22 (SEQ ID 488 509 NO: 105) T59832_T28
(SEQ ID 1527 1548 NO: 106) T59832_T6 (SEQ ID NO: 107) 1905 1926
T59832_T8 (SEQ ID NO: 108) 1257 1278
[1127] Segment cluster T59832_node.sub.--35 (SEQ ID NO:133)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 40
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00293 TABLE 40 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 833 836 NO: 103) T59832_T15 (SEQ
ID 768 771 NO: 104) T59832_T22 (SEQ ID 510 513 NO: 105) T59832_T28
(SEQ ID 1549 1552 NO: 106) T59832_T6 (SEQ ID NO: 107) 1927 1930
T59832_T8 (SEQ ID NO: 108) 1279 1282
[1128] Segment cluster T59832_node.sub.--36 (SEQ ID NO:134)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T28 (SEQ ID NO:106),
T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 41
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00294 TABLE 41 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T59832_T11 (SEQ ID 837 845 NO: 103) T59832_T15 (SEQ
ID 772 780 NO: 104) T59832_T22 (SEQ ID 514 522 NO: 105) T59832_T28
(SEQ ID 1553 1561 NO: 106) T59832_T6 (SEQ ID NO: 107) 1931 1939
T59832_T8 (SEQ ID NO: 108) 1283 1291
[1129] Segment cluster T59832_node.sub.--37 (SEQ ID NO:135)
according to the present invention is supported by 300 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832_T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 42 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00295 TABLE 42 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 846 945 NO: 103) T59832_T15 (SEQ ID 781 880 NO:
104) T59832_T22 (SEQ ID 523 622 NO: 105) T59832_T28 (SEQ ID 1562
1661 NO: 106) T59832_T6 (SEQ ID NO: 107) 1940 2039 T59832_T8 (SEQ
ID NO: 108) 1292 1391
[1130] Segment cluster T59832_node.sub.--38 (SEQ ID NO:136)
according to the present invention is supported by 247 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T28 (SEQ ID NO:106), T59832_T6 (SEQ ID
NO:107) and T59832_T8 (SEQ ID NO:108). Table 43 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00296 TABLE 43 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 946 1030 NO: 103) T59832_T15 (SEQ ID 881 965 NO:
104) T59832_T22 (SEQ ID 623 707 NO: 105) T59832_T28 (SEQ ID 1662
1746 NO: 106) T59832_T6 (SEQ ID NO: 107) 2040 2124 T59832_T8 (SEQ
ID NO: 108) 1392 1476
[1131] Segment cluster T59832_node.sub.--4 (SEQ ID NO:137)
according to the present invention is supported by 296 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID
NO:108). Table 44 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00297 TABLE 44 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T11 (SEQ ID 173 223 NO: 103)
T59832_T15 (SEQ ID 173 223 NO: 104) T59832_T22 (SEQ ID 173 223 NO:
105) T59832_T6 (SEQ ID NO: 107) 173 223 T59832_T8 (SEQ ID NO: 108)
173 223
[1132] Segment cluster T59832_node.sub.--5 (SEQ ID NO:138)
according to the present invention is supported by 305 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T22
(SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID
NO:108). Table 45 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00298 TABLE 45 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T11 (SEQ ID 224 259 NO: 103)
T59832_T15 (SEQ ID 224 259 NO: 104) T59832_T22 (SEQ ID 224 259 NO:
105) T59832_T6 (SEQ ID NO: 107) 224 259 T59832_T8 (SEQ ID NO: 108)
224 259
[1133] Segment cluster T59832 node.sub.--6 (SEQ ID NO:139)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T22 (SEQ ID NO:105), T59832_T6 (SEQ ID NO:107) and
T59832_T8 (SEQ ID NO:108). Table 46 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00299 TABLE 46 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T59832_T11 (SEQ ID 260 280 NO: 103) T59832_T15 (SEQ ID 260 280 NO:
104) T59832_T22 (SEQ ID 260 280 NO: 105) T59832_T6 (SEQ ID NO: 107)
260 280 T59832_T8 (SEQ ID NO: 108) 260 280
[1134] Segment cluster T59832_node.sub.--8 (SEQ ID NO:140)
according to the present invention can be found in the following
transcript(s): T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID
NO:104), T59832_T6 (SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108).
Table 47 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00300 TABLE 47 Segment
location on transcripts Segment Segment Transcript name starting
position ending position T59832_T11 (SEQ ID 281 301 NO: 103)
T59832_T15 (SEQ ID 281 301 NO: 104) T59832_T6 (SEQ ID NO: 107) 1347
1367 T59832_T8 (SEQ ID NO: 108) 281 301
[1135] Segment cluster T59832_node.sub.--9 (SEQ ID NO:141)
according to the present invention is supported by 330 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
T59832_T11 (SEQ ID NO:103), T59832_T15 (SEQ ID NO:104), T59832_T6
(SEQ ID NO:107) and T59832_T8 (SEQ ID NO:108). Table 48 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00301 TABLE 48 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T59832 T11 (SEQ ID 302 337 NO: 103) T59832_T15 (SEQ ID 302 337 NO:
104) T59832_T6 (SEQ ID NO: 107) 1368 1403 T59832_T8 (SEQ ID NO:
108) 302 337
[1136] Variant protein alignment to the previously known
protein:
[1137] Sequence name: /tmp/YQPBtaxsLQ/JxSZR3ZR2p:GILT_HUMAN (SEQ ID
NO:142)
[1138] Sequence Documentation:
[1139] Alignment of: T59832_P5 (SEQ ID NO:143).times.GILT_HUMAN
(SEQ ID NO:142).
[1140] Alignment segment 1/1: TABLE-US-00302 Quality: 429.00
Escore: 0 Matching length: 46 Total length: 46 Matching Percent
97.83 Matching Percent Identity: 97.83 Similarity: Total Percent
Similarity: 97.83 Total Percent Identity: 97.83 Gaps: 0
TABLE-US-00022 Alignment: TABLE-US-00303 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKVG 46
|||||||||||||||||||||||||||||||||||||||||||| | 12
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTG 57
[1141] Sequence name: /tmp/9 HrQ57oZG0/ugNVzp0I7X:GILT_HUMAN (SEQ
ID NO:142)
[1142] Sequence Documentation:
[1143] Alignment of: T59832_P7 (SEQ ID NO:144).times.GILT_HUMAN
(SEQ ID NO:142).
[1144] Alignment segment 1/1: TABLE-US-00304 Quality: 2110.00
Escore: 0 Matching length: 212 Total length: 212 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00023 Alignment: TABLE-US-00305 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 12
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 61 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 62
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 111 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 112
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 161 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 162
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 211.201
PHEYVPWVTVNG 212 |||||||||||| 212 PHEYVPWVTVNG 223
[1145] Sequence name: /tmp/9 HrQ57oZG0/ugNVzp0I7X:BAC98466 (SEQ ID
NO:848)
[1146] Sequence Documentation:
[1147] Alignment of: T59832_P7 (SEQ ID NO:144).times.BAC98466 (SEQ
ID NO:848).
[1148] Alignment segment 1/1: TABLE-US-00306 Quality: 2110.00
Escore: 0 Matching length: 212 Total length: 212 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00024 Alignment: TABLE-US-00307 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200.201
PHEYVPWVTVNG 212 |||||||||||| 201 PHEYVPWVTVNG 212
[1149] Sequence name: /tmp/9 HrQ57oZG0/ugNVzp0I7X:BAC85622 (SEQ ID
NO:849)
[1150] Sequence Documentation:
[1151] Alignment of: T59832_P7 (SEQ ID NO:144).times.BAC85622 (SEQ
ID NO:849).
[1152] Alignment segment 1/1: TABLE-US-00308 Quality: 1496.00
Escore: 0 Matching length: 148 Total length: 148 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00025 Alignment: TABLE-US-00309 . . . 91
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDME 140
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDME 50 . . . 141
LAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHA 190
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
LAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHA 100 . . . 191
NAQRTDALQPPHEYVPWVTVNGVRIFLALSLTLIVPWSQGWTRQRDQR 238
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
NAQRTDALQPPHEYVPWVTVNGVRIFLALSLTLIVPWSQGWTRQRDQR 148
[1153] Sequence name: /tmp/9 HrQ57oZG0/ugNVzp0I7X:Q8WU77 (SEQ ID
NO:850)
[1154] Sequence Documentation:
[1155] Alignment of: T59832_P7 (SEQ ID NO:144).times.Q8WU77 (SEQ ID
NO:850).
[1156] Alignment segment 1/1: TABLE-US-00310 Quality: 21-10.00
Escore: 0 Matching length: 212 Total length: 212 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00026 Alignment: TABLE-US-00311 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 . . . 201
PHEYVPWVTVNG 212 |||||||||||| 201 PHEYVPWVTVNG 212
[1157] Sequence name: /tmp/lttCiW30od/feIXLDs4rU:GILT_HUMAN (SEQ ID
NO:142)
[1158] Sequence Documentation:
[1159] Alignment of: T59832_P9 (SEQ ID NO:145).times.GILT_HUMAN
(SEQ ID NO:142).
[1160] Alignment segment 1/1: TABLE-US-00312 Quality: 2016.00
Escore: 0 Matching length: 203 Total length: 203 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00027 Alignment: TABLE-US-00313 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDEFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 12
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 61 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 62
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 111 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 112
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 161 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 162
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 211 201 PHE 203
||| 212 PHE 214
[1161] Sequence name: /tmp/lttCiW30od/feIXLDs4rU:BAC98466 (SEQ ID
NO:848)
[1162] Sequence Documentation:
[1163] Alignment of: T59832_P9 (SEQ ID NO:145).times.BAC98466 (SEQ
ID NO:848).
[1164] Alignment segment 1/1: TABLE-US-00314 Quality: 2016.00
Escore: 0 Matching length: 203 Total length: 203 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00028 Alignment: TABLE-US-00315 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 201 PHE 203
||| 201 PHE 203
[1165] Sequence name: /tmp/lttCiW30od/feIXLDs4rU:BAC85622 (SEQ ID
NO:849)
[1166] Sequence Documentation:
[1167] Alignment of: T59832_P9 (SEQ ID NO:145).times.BAC85622 (SEQ
ID NO:849).
[1168] Alignment segment 1/1: TABLE-US-00316 Quality: 1145.00
Escore: 0 Matching length: 113 Total length: 113 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00029 Alignment: TABLE-US-00317 . . . 91
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKENKVEACVLDELDME 140
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDME 50 . . . 141
LAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHA 190
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
LAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHA 100.191
NAQRTDALQPPHE 203 ||||||||||||| 101 NAQRTDALQPPHE 113
[1169] Sequence name: /tmp/lttCiW30od/felXLDs4rU:Q8WU77 (SEQ ID
NO:850)
[1170] Sequence Documentation:
[1171] Alignment of: T59832_P9 (SEQ ID NO:145).times.Q8WU77 (SEQ ID
NO:850).
[1172] Alignment segment 1/1: TABLE-US-00318 Quality: 2016.00
Escore: 0 Matching length: 203 Total length: 203 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00030 Alignment: TABLE-US-00319 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 201 PHE 203
||| 201 PHE 203
[1173] Sequence name: /tmp/sIHTwdduiK/ToMKmEJiZc:GILT_HUMAN (SEQ ID
NO:142)
[1174] Sequence Documentation:
[1175] Alignment of: T59832_P12 (SEQ ID NO:146).times.GILT_HUMAN
(SEQ ID NO:142).
[1176] Alignment segment 1/1: TABLE-US-00320 Quality: 2084.00
Escore: 0 Matching length: 219 Total length: 250 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 87.60 Total Percent Identity: 87.60 Gaps: 1
TABLE-US-00031 Alignment: TABLE-US-00321 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 12
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 61 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 62
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 111 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVE.................... 130
|||||||||||||||||||||||||||||| 112
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 161 . . . 131
...........CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 169
|||||||||||||||||||||||||||||||||||||||162
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 211 . . . 170
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 219
|||||||||||||||||||||||||||||||||||||||||||||||||| 212
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 261
[1177] Sequence name: /tmp/sIHTwdduiK/ToMKmEJiZc:BAC85622 (SEQ ID
NO:849)
[1178] Sequence Documentation:
[1179] Alignment of: T59832_P12 (SEQ ID NO:146).times.BAC85622 (SEQ
ID NO:849).
[1180] Alignment segment 1/1: TABLE-US-00322 Quality: 835.00
Escore: 0 Matching length: 91 Total length: 122 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 74.59 Total Percent Identity: 74.59 Gaps: 1
TABLE-US-00032 Alignment: TABLE-US-00323 . . . 91
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVE. . . . . . . . .. 130
|||||||||||||||||||||||||||||||||||||||| 1
MEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDME 50 . . . 131
.....................CLQLYAPGLSPDTIMECAMGDRGMQLMHA 159
||||||||||||||||||||||||||||| 51
LAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHA 100 . . . 160
NAQRTDALQPPHEYVPWVTVNG 181 |||||||||||||||||||||| 101
NAQRTDALQPPHEYVPWVTVNG 122
[1181] Sequence name: /tmp/sIHTwdduiK/ToMKmEJiZc:Q8WU77 (SEQ ID
NO:850)
[1182] Sequence Documentation:
[1183] Alignment of: T59832_P12 (SEQ ID NO:146).times.Q8WU77 (SEQ
ID NO:850).
[1184] Alignment segment 1/1: TABLE-US-00324 Quality: 2084.00
Escore: 0 Matching length: 219 Total length: 250 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 87.60 Total Percent Identity: 87.60 Gaps: 1
TABLE-US-00033 Alignment: TABLE-US-00325 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVE.................... 130
||||||||||||||||||||||||||||||| 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 131
...........CLQLYAPGLSPDTIMECAMGDRCMQLMHANAQRTDALQP 169
|||||||||||||||||||||||||||||||||||||||151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 . . . 170
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 219
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 250
[1185] Sequence name: /tmp/LH4xf8J65f/a95JQoTfNB:GILT_HUMAN (SEQ ID
NO:142)
[1186] Sequence Documentation:
[1187] Alignment of: T59832_P18 (SEQ ID NO:147).times.GILT_HUMAN
(SEQ ID NO:142).
[1188] Alignment segment 1/1: TABLE-US-00326 Quality: 1222.00
Escore: 0 Matching length: 133 Total length: 250 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 53.20 Total Percent Identity: 53.20 Gaps: 1
TABLE-US-00034 Alignment: TABLE-US-00327 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK . . . 44
|||||||||||||||||||||||||||||||||||||||||||| 12
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 61 . . . 44
.................................................. 44 62
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 111 . . . 44
.................................................. 44 112
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 161 . . . 45
...........CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 83
|||||||||||||||||||||||||||||||||||||||162
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 211 . . . 84
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 133
|||||||||||||||||||||||||||||||||||||||||||||||||| 212
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 261
[1189] Sequence name: /tmp/LH4xf8J65f/a95JQoTfNB:Q8WU77 (SEQ ID
NO:850)
[1190] Sequence Documentation:
[1191] Alignment of: T59832_P18 (SEQ ID NO:147).times.Q8WU77 (SEQ
ID NO:850).
[1192] Alignment segment 1/1: TABLE-US-00328 Quality: 1222.00
Escore: 0 Matching length: 133 Total length: 250 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 53.20 Total Percent Identity: 53.20 Gaps: 1
TABLE-US-00035 Alignment: TABLE-US-00329 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK . . . 44
|||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 44
.................................................. 44 51
RGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVP 100 . . . 44
.................................................. 44 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 45
...........CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 83
|||||||||||||||||||||||||||||||||||||||151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 . . . 84
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 133
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 250
[1193] Sequence name: /tmp/LH4xf8J65f/a95JQoTfNB:Q8NEI4 (SEQ ID
NO:851)
[1194] Sequence Documentation:
[1195] Alignment of: T59832_P18 (SEQ ID NO:147).times.Q8NEI4 (SEQ
ID NO:851).
[1196] Alignment segment 1/1: TABLE-US-00330 Quality: 1222.00
Escore: 0 Matching length: 133 Total length: 250 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 53.20 Total Percent Identity: 53.20 Gaps: 1
TABLE-US-00036 Alignment: TABLE-US-00331 . . . 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYK . . . 44
|||||||||||||||||||||||||||||||||||||||||||| 1
MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYL 50 . . . 44
.................................................. 44 51
RGPLKKSNAPLVNVTLYYEALCGGCQAFLIRELFPTWLLVMEILNVTLVP 100 . . . 44
.................................................. 44 101
YGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCME 150 . . . 45
...........CLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 83
|||||||||||||||||||||||||||||||||||||||151
EFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQP 200 . . . 84
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 133
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
PHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSSLRSVCFK 250
Expression of Gamma-Interferon Inducible Lysosomal Thiol Reductase
(GILT) T59832 Transcripts Which are Detectable by Amplicon as
Depicted in Sequence Name T59832junc6-25-26 (SEQ ID NO:854) in
Normal and Cancerous Breast Tissues
[1197] Expression of gamma-interferon inducible lysosomal thiol
reductase (GILT) transcripts detectable by or according to
junc6-25-26, T59832junc6-25-26 (SEQ ID NO:854) amplicon and primers
T59832junc6-25-26F (SEQ ID NO:852) T59832junc6-25-26R (SEQ ID
NO:853) was measured by real time PCR. In parallel the expression
of four housekeeping genes--PBGD (GenBank Accession No. BC019323
(SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1
(GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925)), G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID
NO:918); G6PD-amplicon (SEQ ID NO:921)), was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the normal
post-mortem (PM) samples (Sample Nos. 56-60, 63-67, Table 1 above,
"Tissue samples in testing panel", above), to obtain a value of
fold up-regulation for each sample relative to median of the normal
PM samples.
[1198] FIG. 18 is a histogram showing over expression of the
above-indicated gamma-interferon inducible lysosomal thiol
reductase (GILT) transcripts in cancerous breast samples relative
to the normal samples.
[1199] As is evident from FIG. 18, the expression of
gamma-interferon inducible lysosomal thiol reductase (GILT)
transcripts detectable by the above amplicon(s) in cancer samples
was higher in a few samples than in the non-cancerous samples
(Sample Nos. 56-60, 63-67, Table 1 above, "Tissue samples in
testing panel"). Notably an over-expression of at least 7 fold was
found in 3 out of 28 adenocarcinoma samples.
[1200] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair:
T59832junc6-25-26F forward primer (SEQ ID NO:852); and
T59832junc6-25-26R reverse primer (SEQ ID NO:853).
[1201] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: T59832junc6-25-26 (SEQ ID NO:854). TABLE-US-00332 Forward
primer T59832junc6-25-26F: (SEQ ID NO: 852)
CCACCAGTTAACTACAAGTGCCTG Reverse primer T59832junc6-25-26R: (SEQ ID
NO: 853) GCGTGCATGAGCTGCATG Amplicon T59832junc6-25-26: (SEQ ID NO:
854) CCACCAGTTAACTACAAGTGCCTGCAGCTCTACGCCCCAGGGCTGTCGCCAGACAC
TATCATGGAGTGTGCAATGGGGGACCGCGGCATGCAGCTCATGCACGC
Description for Cluster HUMGRP5E
[1202] Cluster HUMGRP5E features 2 transcript(s) and 5 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00333 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HUMGRP5E_T4 148 HUMGRP5E_T5 149
[1203] TABLE-US-00334 TABLE 2 Segments of interest Segment Name
Sequence ID No. HUMGRP5E_node.sub.--0 150 HUMGRP5E_node.sub.--2 151
HUMGRP5E_node.sub.--8 152 HUMGRP5E_node.sub.--3 153
HUMGRP5E_node.sub.--7 154
[1204] TABLE-US-00335 TABLE 3 Proteins of interest Protein Name
Sequence ID No. HUMGRP5E_P4 156 HUMGRP5E_P5 157
[1205] These sequences are variants of the known protein
Gastrin-releasing peptide precursor (SEQ ID NO: 155) (SwissProt
accession identifier GRP_HUMAN; known also according to the
synonyms GRP; GRP-10), SEQ ID NO: 155, referred to herein as the
previously known protein.
[1206] Gastrin-releasing peptide is known or believed to have the
following function(s): stimulates gastrin release as well as other
gastrointestinal hormones. The sequence for protein
Gastrin-releasing peptide precursor (SEQ ID NO:155) is given at the
end of the application, as "Gastrin-releasing peptide precursor
(SEQ ID NO:155) amino acid sequence". Known polymorphisms for this
sequence are as shown in Table 4. TABLE-US-00336 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 4 S->R
[1207] Protein Gastrin-releasing peptide localization is believed
to be Secreted.
[1208] The previously known protein also has the following
indication(s) and/or potential therapeutic use(s): Diabetes, Type
II. It has been investigated for clinical/therapeutic use in
humans, for example as a target for an antibody or small molecule,
and/or as a direct therapeutic; available information related to
these investigations is as follows. Potential pharmaceutically
related or therapeutically related activity or activities of the
previously known protein are as follows: Bombesin antagonist;
Insulinotropin agonist. A therapeutic role for a protein
represented by the cluster has been predicted. The cluster was
assigned this field because there was information in the drug
database or the public databases (e.g., described herein above)
that this protein, or part thereof, is used or can be used for a
potential therapeutic indication: Anorectic/Antiobesity; Releasing
hormone; Anticancer; Respiratory; Antidiabetic.
[1209] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: signal
transduction; neuropeptide signaling pathway, which are
annotation(s) related to Biological Process; growth factor, which
are annotation(s) related to Molecular Function; and secreted,
which are annotation(s) related to Cellular Component.
[1210] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from; or
Locuslink, available from ncbi.nim.nih.gov/projects/LocusLink/.
[1211] As noted above, cluster HUMGRP5E features 2 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Gastrin-releasing
peptide precursor (SEQ ID NO:155). A description of each variant
protein according to the present invention is now provided.
[1212] Variant protein HUMGRP5E_P4 (SEQ ID NO:156) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HUMGRP5E_T4 (SEQ ID
NO:148). An alignment is given to the known protein
(Gastrin-releasing peptide precursor (SEQ ID NO:155)) at the end of
the application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1213] Comparison report between HUMGRP5E_P4 (SEQ ID NO:156) and
GRP_HUMAN (SEQ ID NO:155):
[1214] 1.An isolated chimeric polypeptide encoding for HUMGRP5E_P4
(SEQ ID NO:156), comprising a first amino acid sequence being at
least 90% homologous to
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG
ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED
SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN (SEQ
ID NO:155), which also corresponds to amino acids 1-127 of
HUMGRP5E_P4 (SEQ ID NO:156), and a second amino acid sequence being
at least 90% homologous to GSQREGRNPQLNQQ corresponding to amino
acids 135-148 of GRP_HUMAN (SEQ ID NO:155), which also corresponds
to amino acids 128-141 of HUMGRP5E_P4 (SEQ ID NO:156), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1215] 2.An isolated chimeric polypeptide encoding for an edge
portion of HUMGRP5E_P4 (SEQ ID NO:156), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise KG, having a structure as follows: a sequence
starting from any of amino acid numbers 127-x to 127; and ending at
any of amino acid numbers 128+((n-2)-x), in which x varies from 0
to n-2.
[1216] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1217] Variant protein HUMGRP5E_P4 (SEQ ID NO:156) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 5, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMGRP5E_P4 (SEQ ID NO:156)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00337 TABLE 5
Amino acid mutations SNP position(s) on amino acid Alternative
sequence amino acid(s) Previously known SNP? 4 S->R Yes
[1218] Variant protein HUMGRP5E_P4 (SEQ ID NO:156) is encoded by
the following transcript(s): HUMGRP5E_T4 (SEQ ID NO:148), for which
the sequence(s) is/are given at the end of the application. The
coding portion of transcript HUMGRP5E_T4, (SEQ ID NO:148) is shown
in bold; this coding portion starts at position 622 and ends at
position 1044. The transcript also has the following SNPs as listed
in Table 6 (given according to their position on the nucleotide
sequence, with the alternative nucleic acid listed; the last column
indicates whether the SNP is known or not; the presence of known
SNPs in variant protein HUMGRP5E_P4 (SEQ ID NO:156) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00338 TABLE 6 Nucleic
acid SNPs SNP position on nucleotide Alternative sequence nucleic
acid Previously known SNP? 541->T No 542 G->T No 631 A->C
Yes 672 G->A Yes 1340 C->No 1340 C->A No 1341 A->No
1341 A->G No
[1219] Variant protein HUMGRP5E_P5 (SEQ ID NO:157) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HUMGRP5E_T5 (SEQ ID
NO:149). An alignment is given to the known protein
(Gastrin-releasing peptide precursor (SEQ ID NO:155)) at the end of
the application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1220] Comparison report between HUMGRP5E_P5 (SEQ ID NO:157) and
GRP_HUMAN (SEQ ID NO:155):
[1221] 1. An isolated chimeric polypeptide encoding for HUMGRP5E_P5
(SEQ ID NO:157), comprising a first amino acid sequence being at
least 90% homologous to
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLMGKKSTG
ESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSED
SSNFKDVGSKGK corresponding to amino acids 1-127 of GRP_HUMAN (SEQ
ID NO:155), which also corresponds to amino acids 1-127 of
HUMGRP5E_P5 (SEQ ID NO:157), and a second amino acid sequence being
at least 70%, optionally at least 80%, preferably at least 85%,
more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence DSLLQVLNVKEGTPS
(SEQ ID NO:1017) corresponding to amino acids 128-142 of
HUMGRP5E_P5 (SEQ ID NO:157), wherein said first and second amino
acid sequences are contiguous and in a sequential order.
[1222] 2. An isolated polypeptide encoding for a tail of
HUMGRP5E_P5 (SEQ ID NO:157), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence DSLLQVLNVKEGTPS (SEQ ID
NO:1017) in HUMGRP5E_P5 (SEQ ID NO:157).
[1223] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1224] Variant protein HUMGRP5E_P5 (SEQ ID NO:157) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMGRP5E_P5 (SEQ ID NO:157)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00339 TABLE 7
Amino acid mutations SNP position(s) on amino acid Alternative
sequence amino acid(s) Previously known SNP? 4 S->R Yes
[1225] Variant protein HUMGRP5E_P5 (SEQ ID NO:157) is encoded by
the following transcript(s): HUMGRP5E_T5 (SEQ ID NO:149), for which
the sequence(s) is/are given at the end of the application. The
coding portion of transcript HUMGRP5E_T5 (SEQ ID NO:149) is shown
in bold; this coding portion starts at position 622 and ends at
position 1047. The transcript also has the following SNPs as listed
in Table 8 (given according to their position on the nucleotide
sequence, with the alternative nucleic acid listed; the last column
indicates whether the SNP is known or not; the presence of known
SNPs in variant protein HUMGRP5E_P5 (SEQ ID NO:157) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00340 TABLE 8 Nucleic
acid SNPs SNP position on nucleotide Alternative sequence nucleic
acid Previously known SNP? 541->T No 542 G->T No 631 A->C
Yes 672 G->A Yes 1354 C->No 1354 C->A No 1355 A->No
1355 A->G No
[1226] As noted above, cluster HUMGRP5E features 5 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1227] Segment cluster HUMGRP5E_node.sub.--0 (SEQ ID NO:150)
according to the present invention is supported by 21 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMGRP5E_T4 (SEQ ID NO:148) and HUMGRP5E_T5 (SEQ ID NO:149). Table
9 below describes the starting and ending position of this segment
on each transcript. TABLE-US-00341 TABLE 9 Segment location on
transcripts Segment starting Segment ending Transcript name
position position HUMGRP5E_T4 1 760 (SEQ ID NO: 148) HUMGRP5E_T5 1
760 (SEQ ID NO: 149)
[1228] Segment cluster HUMGRP5E_node.sub.--2 (SEQ ID NO:151)
according to the present invention is supported by 27 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMGRP5E_T4 (SEQ ID NO:148) and HUMGRP5E_T5 (SEQ ID NO:149). Table
10 below describes the starting and ending position of this segment
on each transcript. TABLE-US-00342 TABLE 10 Segment location on
transcripts Segment starting Segment ending Transcript name
position position HUMGRP5E_T4 761 984 (SEQ ID NO: 148) HUMGRP5E_T5
761 984 (SEQ ID NO: 149)
[1229] Segment cluster HUMGRP5E_node.sub.--8 (SEQ ID NO:152)
according to the present invention is supported by 26 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMGRP5E_T4 (SEQ ID NO:148) and HUMGRP5E_T5 (SEQ ID NO:149). Table
11 below describes the starting and ending position of this segment
on each transcript. TABLE-US-00343 TABLE 11 Segment location on
transcripts Segment starting Segment ending Transcript name
position position HUMGRP5E_T4 1004 1362 (SEQ ID NO: 148)
HUMGRP5E_T5 1018 1376 (SEQ ID NO: 149)
[1230] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1231] Segment cluster HUMGRP5E_node.sub.--3 (SEQ ID NO:153)
according to the present invention can be found in the following
transcript(s): HUMGRP5E_T4 (SEQ ID NO:148) and HUMGRP5E_T5 (SEQ ID
NO:149). Table 12 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00344 TABLE 12 Segment
location on transcripts Segment starting Segment ending Transcript
name position position HUMGRP5E_T4 985 1003 (SEQ ID NO: 148)
HUMGRP5E_T5 985 1003 (SEQ ID NO: 149)
[1232] Segment cluster HUMGRP5E_node.sub.--7 (SEQ ID NO:154)
according to the present invention can be found in the following
transcript(s): HUMGRP5E_T5 (SEQ ID NO:149). Table 13 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00345 TABLE 13 Segment location on transcripts
Segement Segment Transcript name starting position ending position
HUMGRP5E_T5 1004 1017 (SEQ ID NO:149)
[1233] Variant protein alignment to the previously known
protein:
[1234] Sequence name: /tmp/412zs2 mwyT/B0wjOUAX0d:GRP_HUMAN (SEQ ID
NO:155)
[1235] Sequence Documentation:
[1236] Alignment of: HUMGRP5E_P4 (SEQ ID NO:156).times.GRP_HUMAN
(SEQ ID NO:155).
[1237] Alignment segment 1/1: TABLE-US-00346 Quality: 1291.00
Escore: 0 Matching length: 141 Total length: 148 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 95.27 Total Percent Identity: 95.27 Gaps: 1
TABLE-US-00037 Alignment: TABLE-US-00347 . . . 1
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLM 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLM 50 . . . 51
GKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQ 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
GKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQ 100 . . . 101
PKALGNQQPSWDSEDSSNFKDVGSKGK.......GSQREGRNPQLNQQ 141
||||||||||||||||||||||||||||||||||||||||| 101
PKALGNQQPSWDSEDSSNFKDVGSKGKVGRLSAPGSQREGRNPQLNQQ 148
[1238] Sequence name: /tmp/1me9Idnvfv/KbP5io8PtU:GRP_HUMAN (SEQ ID
NO:155)
[1239] Sequence Documentation:
[1240] Alignment of: HUMGRP5E_P5 (SEQ ID NO:157).times.GRP HUMAN
(SEQ ID NO:155).
[1241] Alignment segment 1/1: TABLE-US-00348 Quality: 1248.00
Escore: 0 Matching length: 127 Total length: 127 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00038 Alignment: TABLE-US-00349 . . . 1
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLM 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRGSELPLVLLALVLCLAPRGRAVPLPAGGGTVLTKMYPRGNHWAVGHLM 50 . . . 51
GKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQ 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
GKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQ 100 . . . 101
PKALGNQQPSWDSEDSSNFKDVGSKGK 127 ||||||||||||||||||||||||||| 101
4PKALGNQQPSWDSEDSSNFKDVGSKGK 127
Expression of GRP_HUMAN-Gastrin-Releasing Peptide (HUMGRP5E)
Transcripts, Which are Detectable by Amplicon, as Depicted in
Sequence Name HUMGRP5Ejunc3-7 (SEQ ID NO:857) in Normal and
Cancerous Breast Tissues
[1242] Expression of GRP_HUMAN-gastrin-releasing peptide
transcripts detectable by or according to junc3-7, HUMGRP5Ejunc3-7
(SEQ ID NO:857) amplicon(s) and HUMGRP5Ejunc3-7F (SEQ ID NO:855)
and HUMGRP5Ejunc3-7R (SEQ ID NO:856) primers was measured by real
time PCR. In parallel the expression of four housekeeping genes
PBGD (GenBank Accession No. BC019323 (SEQ ID NO:926);
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), and SDHA (GenBank Accession No. NM.sub.--004168. (SEQ
ID NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925), G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67 Table 1, "Tissue samples in testing panel"), to obtain
a value of fold up-regulation for each sample relative to median of
the normal PM samples.
[1243] FIG. 19 is a histogram showing over expression of the
above-indicated GRP_HUMAN-gastrin-releasing peptide transcripts in
cancerous breast samples relative to the normal samples. Values
represent the average of duplicate experiments. Error bars indicate
the minimal and maximal values obtained.
[1244] As is evident from FIG. 19, the expression of
GRP_HUMAN-gastrin-releasing peptide transcripts detectable by the
above amplicon(s) in cancer samples was significantly higher than
in the non-cancerous samples (Sample Nos. 56-60, 63-67, Table 1
"Tissue samples in testing panel"). Notably an over-expression of
at least 5 fold was found in 12 out of 28 adenocarcinoma
samples.
[1245] Statistical analysis was applied to verify the significance
of these results, as described below.
[1246] The P value for the difference in the expression levels of
GRP_HUMAN-gastrin-releasing peptide transcripts detectable by the
above amplicon(s) in breast cancer samples versus the normal tissue
samples was determined by T test as 7.22E-04.
[1247] Threshold of 5 fold over expression was found to
differentiate between cancer and normal samples with P value of
1.12E-02 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1248] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair:
HUMGRP5Ejunc3-7F forward primer (SEQ ID NO:855); and
HUMGRP5Ejunc3-7R reverse primer (SEQ ID NO:856).
[1249] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: TABLE-US-00350 HUMGRP5Ejunc3-7. (SEQ ID NO:857)
HUMGRP5Ejunc3-7F (SEQ ID NO:855) ACCAGCCACCTCAACCCA
HUMGRP5Ejunc3-7R (SEQ ID NO:856) CTGGAGCAGAGAGTCTTTGCCT
HUMGRP5Ejunc3-7 (SEQ ID NO:857)
ACCAGCCACCTCAACCCAAGGCCCTGGGCAATCAGCAGCCTTCGTGGGAT
TCAGAGGATAGCAGCAACTTCAAAGATGTAGGTTCAAAAGGCAAAGACTCTCTGCTCCAG
[1250] Expression of GRP_HUMAN-Gastrin-Releasing Peptide (HUMGRP5E)
Transcripts, Which are Detectable by Amplicon, as Depicted in
Sequence Name HUMGRP5Ejunc3-7 (SEQ ID NO:857) in Different Normal
Tissues
[1251] Expression of GRP_HUMAN-gastrin-releasing peptide
transcripts detectable by or according to HUMGRP5E junc3-7
amplicon(s) and HUMGRP5E junc3-7F and HUMGRP5E junc3-7R was
measured by real time PCR. In parallel the expression of four
housekeeping genes--RPL19 (GenBank Accession No. NM.sub.--000981
(SEQ ID NO:934); RPL19 amplicon (SEQ ID NO:937), TATA box (GenBank
Accession No. NM.sub.--003194 (SEQ ID NO:938); TATA amplicon (SEQ
ID NO:941)), UBC (GenBank Accession No. BC000449 (SEQ ID NO:942);
amplicon--Ubiquitin-amplicon (SEQ ID NO:945)) and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the breast samples
(Sample Nos. 33-35 above, Table 2, "Tissue samples in normal
panel"), to obtain a value of relative expression of each sample
relative to median of the breast samples. Primers and amplicon are
as above.
[1252] The results are presented in FIG. 20, demonstrating the
expression of GRP_HUMAN--gastrin-releasing peptide (HUMGRP5E)
transcripts, which are detectable by amplicon, as depicted in
sequence name HUMGRP5Ejunc3-7 (SEQ ID NO:857), in different normal
tissues.
Description for Cluster AA1 55578
[1253] Cluster AA1 55578 features 4 transcript(s) and 15 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00351 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. AA1 55578_PEA.sub.--1_T10 158
AA155578_PEA.sub.--1_T12 159AA155578_PEA.sub.--1_T13
160AA155578_PEA.sub.--1_T8 161
[1254] TABLE-US-00352 TABLE 2 Segments of interest Segment Name
Sequence ID No. AA155578_PEA.sub.--1_node.sub.--11
162AA155578_PEA.sub.--1_node.sub.--12 163
AA155578_PEA.sub.--1_node.sub.--14
164AA155578_PEA.sub.--1_node.sub.--19 165
AA155578_PEA.sub.--1_node.sub.--21 166
AA155578_PEA.sub.--1_node.sub.--23 167
AA155578_PEA.sub.--1_node.sub.--24 168
AA155578_PEA.sub.--1_node.sub.--25 169
AA155578_PEA.sub.--1_node.sub.--4 170
AA155578_PEA.sub.--1_node.sub.--7 171
AA155578_PEA.sub.--1_node.sub.--15 172
AA155578_PEA.sub.--1_node.sub.--18
173AA155578_PEA.sub.--1_node.sub.--22
174AA155578_PEA.sub.--1_node.sub.--6 175
AA155578_PEA.sub.--1_node.sub.--8 176
[1255] TABLE-US-00353 TABLE 3 Proteins of interest Protein Name
Sequence ID No. AA155578_PEA.sub.--1_P4 178 AA155578_PEA.sub.--1_P6
179 AA155578_PEA.sub.--1_P8 180 AA155578_PEA.sub.--1_P9 181
[1256] These sequences are variants of the known protein Kallikrein
10 precursor (SEQ ID NO:177) (SwissProt accession identifier
KLKA_HUMAN; known also according to the synonyms EC 3.4.21.-;
Protease serine-like 1; Normal epithelial cell-specific 1), SEQ ID
NO: 177, referred to herein as the previously known protein.
[1257] Protein Kallikrein 10 precursor (SEQ ID NO:177) is known or
believed to have the following function(s): Has a tumor-suppressor
role for NES1 in breast and prostate cancer. The sequence for
protein Kallikrein 10 precursor (SEQ ID NO:177) is given at the end
of the application, as "Kallikrein 10 precursor (SEQ ID NO:177)
amino acid sequence". Known polymorphisms for this sequence are as
shown in Table 4. TABLE-US-00354 TABLE 4 Amino acid mutations for
Known Protein SNP position(s) on amino acid sequence Comment 50
A->S 149 P->L
[1258] Protein Kallikrein 10 precursor (SEQ ID NO:177) localization
is believed to be Secreted (Probable).
[1259] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: proteolysis and
peptidolysis, which are annotation(s) related to Biological
Process; chymotrypsin; trypsin; serine-type peptidase; hydrolase,
which are annotation(s) related to Molecular Function; and
extracellular, which are annotation(s) related to Cellular
Component.
[1260] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1261] Cluster AA155578 can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 21 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[1262] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 21 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues and pancreas carcinoma.
TABLE-US-00355 TABLE 5 Normal tissue distribution Name of Tissue
Number Brain 0 Colon 0 epithelial 17 general 5 head and neck 0 Lung
14 Ovary 0 pancreas 0 prostate 4 Skin 80 stomach 0 Uterus 45
[1263] TABLE-US-00356 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4Brain 1 3.7e-01 1
1.0 3.5e-02 5.1 Colon 6.3e-02 2.9e-02 2.4e-01 2.9 1.6e-01 3.2
epithelial 4.9e-03 2.0e-02 7.5e-04 2.1 2.3e-03 1.9 general 7.8e-07
7.2e-06 3.8e-10 4.8 2.6e-10 4.4 head and neck 1.0e-02 3.5e-02
4.6e-01 2.5 7.5e-01 1.4 Lung 8.5e-01 9.2e-01 1 0.5 1 0.5 Ovary
2.2e-01 1.6e-01 1.0e-02 3.3 1.8e-02 3.4 pancreas 3.3e-01 6.9e-02
3.2e-02 3.7 2.4e-04 10.0 prostate 8.3e-01 8.7e-01 6.7e-01 1.2
7.5e-01 1.1 Skin 6.0e-01 8.1e-01 3.2e-01 1.9 1 0.3 stomach 3.0e-01
2.7e-01 2.5e-01 3.0 1.6e-01 2.3 Uterus 1.8e-01 3.2e-01 5.6e-01 0.8
6.8e-01 0.8
[1264] As noted above, cluster AA1 55578 features 4 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Kallikrein 10 precursor
(SEQ ID NO:177). A description of each variant protein according to
the present invention is now provided.
[1265] Variant protein AA155578_PEA.sub.--1_P4 (SEQ ID NO:178)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
AA155578_PEA.sub.--1_T10 (SEQ ID NO:158). An alignment is given to
the known protein (Kallikrein 10 precursor (SEQ ID NO:177)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1266] Comparison report between AA155578_PEA.sub.--1_P4 (SEQ ID
NO:178) and KLKA_HUMAN (SEQ ID NO:177):
[1267] 1. An isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGAPCARGSQ
PWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDHLLLLQGEQLRRTT
RSWHPKYHQGSGPILPRRTDEHDLMLLKLARP corresponding to amino acids 1-146
of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino
acids 1-146 of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), and a
second amino acid sequence being at least 90% homologous to
YNKGLTCSSITILSPKECEVFYPGWTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGIL
SWGVYPCGSAQHPAVYTQICKYMSWINKVIRSN corresponding to amino acids
184-276 of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to
amino acids 147-239 of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1268] 2. An isolated chimeric polypeptide encoding for an edge
portion of AA155578_PEA.sub.--1_P4 (SEQ ID NO:178), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise PY, having a structure as follows: a
sequence starting from any of amino acid numbers 146-x to 146; and
ending at any of amino acid numbers 147+((n-2)-x), in which x
varies from 0 to n-2.
[1269] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1270] Variant protein AA155578_PEA.sub.--1_P4 (SEQ ID NO:178) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein AA155578_PEA.sub.--1_P4 (SEQ ID
NO:178) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00357
TABLE 7 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 123
Q->R No 145 R->G No 145 R->M Yes 168 Y->No 19 K->No
194 G->No 43 L->S No 50 A->S Yes 60 Q->R No
[1271] Variant protein AA155578_PEA.sub.--1_P4 (SEQ ID NO:178) is
encoded by the following transcript(s): AA155578_PEA.sub.--1_T10
(SEQ ID NO:158), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
AA155578_PEA.sub.--1_T10 SEQ ID NO:158) is shown in bold; this
coding portion starts at position 148 and ends at position 864. The
transcript also has the following SNPs as listed in Table 8 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein AA155578_PEA.sub.--1_P4 (SEQ ID NO:178) sequence provides
support for the deduced sequence variant protein according to the
present invention). TABLE-US-00358 TABLE 8 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 19 C->G Yes 88 G->No 570 A->G Yes 580 A->G
No 581 G->T Yes 651 C->No 729 C->No 733 C->T No 875
G->A No 906 C->A No 907 C->A No 952 C->A No 204
G->No 953 C->A No 994 C->A Yes 1125 C->T Yes 1192
C->T Yes 1330 G->No 1330 G->T Yes 275 T->C No 295
G->T Yes 326 A->G No 444 C->T Yes 465 C->A Yes 483
G->C Yes 515 A->G No
[1272] Variant protein AA155578_PEA.sub.--1_P6 (SEQ ID NO:179)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
AA155578_PEA.sub.--1_T12 (SEQ ID NO: 159). An alignment is given to
the known protein (Kallikrein 10 precursor (SEQ ID NO:177)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1273] Comparison report between AA155578_PEA.sub.--1_P6 (SEQ ID
NO:179) and KLKA_HUMAN (SEQ ID NO:177):
[1274] 1. An isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P6 (SEQ ID NO:179), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSMSGARALAKLLPLLMAQLW corresponding to amino acids 1-29 of
KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino acids
1-29 of AA155578_PEA.sub.--1_P6 (SEQ ID NO:179), and a second amino
acid sequence being at least 90% homologous to
VKYNKGLTCSSITILSPKECEVFYPGWTNNMICAGLDRGQDPCQSDSGGPLVCDETLQ
GILSWGVYPCGSAQHPAVYTQICKYMSWINKVIRSN corresponding to amino acids
182-276 of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to
amino acids 30-124 of AA155578_PEA.sub.--1_P6 (SEQ ID NO:179),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1275] 2. An isolated chimeric polypeptide encoding for an edge
portion of AA155578_PEA.sub.--1_P6 (SEQ ID NO:179), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise WV, having a structure as follows: a
sequence starting from any of amino acid numbers 29-x to 29; and
ending at any of amino acid numbers 30+((n-2)-x), in which x varies
from 0 to n-2.
[1276] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1277] Variant protein AA155578_PEA.sub.--1_P6 (SEQ ID NO:179) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 9, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein AA155578_PEA.sub.--1_P6 (SEQ ID
NO:179) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00359
TABLE 9 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 19
K->No 53 Y->No 79 G->No
[1278] Variant protein AA155578_PEA.sub.--1_P6 (SEQ ID NO:179) is
encoded by the following transcript(s): AA155578_PEA.sub.--1_T12
(SEQ ID NO:159), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) is shown in bold; this
coding portion starts at position 148 and ends at position 519. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein AA155578_PEA.sub.--1_P6 (SEQ ID NO:179) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00360 TABLE 10 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 19 C->G Yes 88 G->No 608 C->A No 649
C->A Yes 780 C->T Yes 847 C->T Yes 985 G->No
985.G->T Yes 204 G->No 306 C->No 384 C->No 388 C->T
No 530 G->A No 561 C->A No 562 C->A No 607 C->A No
[1279] Variant protein AA155578_PEA.sub.--1_P8 (SEQ ID NO:180)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). An alignment is given to
the known protein (Kallikrein 10 precursor (SEQ ID NO:177)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1280] Comparison report between AA155578_PEA.sub.--1_P8 (SEQ ID
NO:180) and KLKA_HUMAN (SEQ ID NO:177):
[1281] 1. An isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSMSGARALAKLLPLLMAQLW corresponding to amino acids 1-29 of
KLKA_HUMAN (SEQ ID NO:177, which also corresponds to amino acids
1-29 of AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence GHCGLE (SEQ ID NO: 1018) corresponding to amino acids
30-35 of AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1282] 2. An isolated polypeptide encoding for a tail of
AA155578_PEA.sub.--1_P8 (SEQ ID NO:180), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence GHCGLE
(SEQ ID NO:1018) in AA155578_PEA.sub.--1_P8 (SEQ ID NO:180).
[1283] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1284] Variant protein AA155578_PEA.sub.--1_P8 (SEQ ID NO:180) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein AA155578_PEA.sub.--1_P8 (SEQ ID
NO:180) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00361
TABLE 11 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 19
K->No
[1285] Variant protein AA155578_PEA.sub.--1_P8 (SEQ ID NO:180) is
encoded by the following transcript(s): AA155578_PEA.sub.--1_T8
(SEQ ID NO:161), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161) is shown in bold; this
coding portion starts at position 285 and ends at position 389. The
transcript also has the following SNPs as listed in Table 12 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein AA155578_PEA.sub.--1_P8 (SEQ ID NO:180) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00362 TABLE 12 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 341 G->No 400 C->T Yes 718 C->No 796
C->No 800 C->T No 942 G->A No 973 C->A No 974 C->A
No 1019 C->A No 1020 C->A No 1061 C->A Yes 1192 C->T
Yes 421 C->A Yes 1259 C->T Yes 1397 G->No 1397 G->T Yes
439 G->C Yes 471 A->G No 526 A->G Yes 536 A->G No 537
G->T Yes 549 C->T Yes 587 T->C No
[1286] Variant protein AA155578_PEA.sub.--1_P9 (SEQ ID NO: 181)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
AA155578_PEA.sub.--1_T13 (SEQ ID NO:160). An alignment is given to
the known protein (Kallikrein 10 precursor (SEQ ID NO:177)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1287] Comparison report between AA155578_PEA.sub.--1_P9 (SEQ ID
NO:181) and KLKA_HUMAN (SEQ ID NO:177):
[1288] 1. An isolated chimeric polypeptide encoding for
AA155578_PEA.sub.--1_P9 (SEQ ID NO:181), comprising a first amino
acid sequence being at least 90% homologous to
MRAPHLHLSMSGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGAPCARGSQ
PWQVSLFNGLSFHCAGVLVDQSWVLTMHCGNK corresponding to amino acids 1-90
of KLKA_HUMAN (SEQ ID NO:177), which also corresponds to amino
acids 1-90 of AA155578_PEA.sub.--1_P9 (SEQ ID NO:181).
[1289] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1290] Variant protein AA155578_PEA.sub.--1_P9 (SEQ ID NO:181) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein AA155578_PEA.sub.--1_P9 (SEQ ID
NO:181) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00363
TABLE 13 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 19
K->No 43 L->S No 50 A->S Yes 60 Q->R No
[1291] Variant protein AA155578_PEA.sub.--1_P9 (SEQ ID NO:181) is
encoded by the following transcript(s): AA155578_PEA.sub.--1_T13
(SEQ ID NO:160), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
AA155578_PEA.sub.--1_T13 (SEQ ID NO:160) is shown in bold; this
coding portion starts at position 148 and ends at position 417. The
transcript also has the following SNPs as listed in Table 14 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein AA155578_PEA.sub.--1_P9 (SEQ ID NO: 181) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00364 TABLE 14 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 19 C->G Yes 88 G->No 204 G->No 275
T->C No 295 G->T Yes 326 A->G No 559 G->C Yes 560
C->G Yes 582 A->G Yes 919 T->A Yes
[1292] As noted above, cluster AA155578 features 15 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1293] Segment cluster AA155578_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:162) according to the present invention is supported by 34
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158) and
AA155578_PEA.sub.--1_T13 (SEQ ID NO: 160). Table 15 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00365 TABLE 15 Segment location on transcripts
Segment starting Segment ending Transcript name position position
AA155578_PEA.sub.--1_T10 236 416 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T13 236 416 (SEQ ID NO: 160)
[1294] Segment cluster AA155578_PEA.sub.--1_node.sub.--12 (SEQ ID
NO: 163) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T13 (SEQ ID NO:160). Table 16
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00366 TABLE 16 Segment location on
transcripts Segment starting Segment ending Transcript name
position position AA155578_PEA.sub.--1_T13 417 935 (SEQ ID NO:
160)
[1295] Segment cluster AA55578_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:164) according to the present invention is supported by 31
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 17 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00367 TABLE 17 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 417 585 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T8 373 541 (SEQ ID NO: 161)
[1296] Segment cluster AA155578_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:165) according to the present invention is supported by 45
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 18 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00368 TABLE 18 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 586 714 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 241 369 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 653 781 (SEQ ID NO: 161)
[1297] Segment cluster AA155578_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:166) according to the present invention is supported by 53
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 19 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00369 TABLE 19 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 715 863 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 370 518 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 782 930 (SEQ ID NO: 161)
[1298] Segment cluster AA155578_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:167) according to the present invention is supported by 71
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 20 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00370 TABLE 20 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 887 1063 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 542 718 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 954 1130 (SEQ ID NO: 161)
[1299] Segment cluster AA155578_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:168) according to the present invention is supported by 52
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 21 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00371 TABLE 21 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 1064 1184 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 719 839 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 1131 1251 (SEQ ID NO: 161)
[1300] Segment cluster AA155578_PEA.sub.--1_node.sub.--25 (SEQ ID
NO:169) according to the present invention is supported by 53
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO: 158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 22 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00372 TABLE 22 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 1185 1397 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 840 1052 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 1252 1464 (SEQ ID NO: 161)
[1301] Segment cluster AA155578_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:170) according to the present invention is supported by 21
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158)
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T13 (SEQ ID NO:160). Table 23 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00373 TABLE 23 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 1 138 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 1 138 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T13 1 138 (SEQ ID NO: 160)
[1302] Segment cluster AA155578_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:171) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 24
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00374 TABLE 24 Segment location on
transcripts Segment Transcript name starting position Segment
ending position AA155578_PEA.sub.--1_T8 92 275 (SEQ ID NO: 161)
[1303] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1304] Segment cluster AA155578_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:172) according to the present invention is supported by 33
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 25
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00375 TABLE 25 Segment location on
transcripts Segment Transcript name starting position Segment
ending position AA155578_PEA.sub.--1_T8 542 647 (SEQ ID NO:
161)
[1305] Segment cluster AA155578_PEA.sub.--1_node.sub.--18 (SEQ ID
NO: 173) according to the present invention can be found in the
following transcript(s): AA155578_PEA.sub.--1_T12 (SEQ ID NO:159)
and AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 26 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00376 TABLE 26 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T12 236 240 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 648 652 (SEQ ID NO: 161)
[1306] Segment cluster AA1155578_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:174) according to the present invention can be found in the
following transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159) and
AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 27 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00377 TABLE 27 Segment location on transcripts
Segment Transcript name starting position Segment ending position
AA155578_PEA.sub.--1_T10 864 886 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 519 541 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T8 931 953 (SEQ ID NO: 161)
[1307] Segment cluster AA155578_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:175) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table 28
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00378 TABLE 28 Segment location on
transcripts Segment Transcript name starting position Segment
ending position AA155578_PEA.sub.--1_T8 1 91 (SEQ ID NO: 161)
[1308] Segment cluster AA155578_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:176) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AA155578_PEA.sub.--1_T10 (SEQ ID NO:158),
AA155578_PEA.sub.--1_T12 (SEQ ID NO:159), AA155578_PEA.sub.--1_T13
(SEQ ID NO:160) and AA155578_PEA.sub.--1_T8 (SEQ ID NO:161). Table
29 below describes the starting and ending position of this segment
on each transcript. TABLE-US-00379 TABLE 29 Segment location on
transcripts Segment Transcript name starting position Segment
ending position AA1 55578_PEA.sub.--1_T10 139 235 (SEQ ID NO: 158)
AA155578_PEA.sub.--1_T12 139 235 (SEQ ID NO: 159)
AA155578_PEA.sub.--1_T13 139 235 (SEQ ID NO: 160)
AA155578_PEA.sub.--1_T8 276 372 (SEQ ID NO: 161)
[1309] Variant protein alignment to the previously known
protein:
[1310] Sequence name: /tmp/4gXdRV0C1z/cQ4LqHmh5A:KLKA_HUMAN (SEQ ID
NO:177)
[1311] Sequence Documentation:
[1312] Alignment of: AA155578_PEA.sub.--1_P4 (SEQ ID
NO:178).times.KLKA_HUMAN (SEQ ID NO:177).
[1313] Alignment segment 1/1: TABLE-US-00380 Quality: 2283.00
Escore: 0 Matching length: 239 Total length: 276 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 86.59 Total Percent Identity: 86.59 Gaps: 1
TABLE-US-00039 Alignment: TABLE-US-00381 . . . 1
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGA 50 . . . 51
PCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDH 100 . . . 101
LLLLQGEQLRRTTRSVVHPKYHQGSGPILPRRTDEHDLMLLKLARP . . . 146
|||||||||||||||||||||||||||||||||||||||||||||| 101
LLLLQGEQLRRTTRSVVHPKYHQGSGPILPRRTDEHDLMLLKLARPVVPG 150 . . . 147
.................................YNKGLTCSSITILSPKE 163
||||||||||||||||| 151
PRVRALQLPYRCAQPGDQCQVAGWGTTAARRVKYNKGLTCSSITILSPKE 200 . . . 164
CEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGILSWGVYPC 213
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
CEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGILSWGVYPC 250 . . . 214
GSAQHPAVYTQICKYMSWINKVIRSN 239 |||||||||||||||||||||||||| 251
GSAQHPAVYTQICKYMSWINKVIRSN 276
[1314] Sequence name: /tmp/3VxcRS97HN/X9ncdxjYQx:KLKA_HUMAN (SEQ ID
NO:177)
[1315] Sequence Documentation:
[1316] Alignment of: AA155578_PEA.sub.--1_P6 (SEQ ID
NO:179).times.KLKA_HUMAN (SEQ ID NO:177).
[1317] Alignment segment 1/1: TABLE-US-00382 Quality: 1140.00
Escore: 0 Matching length: 124 Total length: 276 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 44.93 Total Percent Identity: 44.93 Gaps: 1
TABLE-US-00040 Alignment: TABLE-US-00383 . . . 1
MRAPHLHLSAASGARALAKLLPLLMAQLW..................... 29
||||||||||||||||||||||||||||| 1
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGA 50 . . . 29
.................................................. 29 51
PCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDH 100 . . . 29
.................................................. 29 101
LLLLQGEQLRRTTRSVVHPKYHQGSGPILPRRTDEHDLMLLKLARPVVPG 150 . . . 30
...............................VKYNKGLTCSSITILSPKE 48
||||||||||||||||||| 151
PRVRALQLPYRCAQPGDQCQVAGWGTTAARRVKYNKGLTCSSITILSPKE 200 . . . 49
CEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGILSWGVYPC 98
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
CEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGILSWGVYPC 250 . . . 99
GSAQHPAVYTQICKYMSWINKVIRSN 124 |||||||||||||||||||||||||| 251
GSAQHPAVYTQICKYMSWINKVIRSN 276
[1318] Sequence name: /tmp/LsSdTeu0qX/6IuiCMKTi9:KLKA_HUMAN (SEQ ID
NO:177)
[1319] Sequence Documentation:
[1320] Alignment of: AA155578_PEA.sub.--1_P8 (SEQ ID
NO:180).times.KLKA_HUMAN (SEQ ID NO:177).
[1321] Alignment segment 1/1: TABLE-US-00384 Quality: 279.00
Escore: 0 Matching length: 29 Total length: 29 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00041 Alignment: TABLE-US-00385 . . . 1
MRAPHLHLSAASGARALAKLLPLLMAQLW 29 ||||||||||||||||||||||||||||| 1
MRAPHLHLSAASGARALAKLLPLLMAQLW 29
[1322] Sequence name: /tmp/kcfKGMcF7s/YnKnMy8D1q:KLKA_HUMAN (SEQ ID
NO:177)
[1323] Sequence Documentation:
[1324] Alignment of: AA155578_PEA.sub.--1_P9 (SEQ ID
NO:181).times.KLKA_HUMAN (SEQ ID NO:177)
[1325] Alignment segment 1/1: TABLE-US-00386 Quality: 887.00
Escore: 0 Matching length: 90 Total length: 90 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00042 Alignment: TABLE-US-00387 . . . 1
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRAPHLHLSAASGARALAKLLPLLMAQLWAAEAALLPQNDTRLDPEAYGA 50 . . . 51
PCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNK 90
|||||||||||||||||||||||||||||||||||||||| 51
PCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNK 90
Description for Cluster HSENA78
[1326] Cluster HSENA78 features 1 transcript(s) and 7 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00388 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSENA78_T5 182
[1327] TABLE-US-00389 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSENA78_node.sub.--0 183 HSENA78_node.sub.--2 184
HSENA78_node.sub.--6 185 HSENA78_node.sub.--9 186
HSENA78_node.sub.--3 187 HSENA78_node.sub.--4 188
HSENA78_node.sub.--8 189
[1328] TABLE-US-00390 TABLE 3 Proteins of interest Protein Name
Sequence ID No. HSENA78_P2 191
[1329] These sequences are variants of the known protein Small
inducible cytokine B5 precursor (SEQ ID NO:190) (SwissProt
accession identifier SZ05_HUMAN; known also according to the
synonyms CXCL5; Epithelial-derived neutrophil activating protein
78; Neutrophil-activating peptide ENA-78), SEQ ID NO: 190, referred
to herein as the previously known protein.
[1330] Protein Small inducible cytokine B5 precursor (SEQ ID
NO:190) is known or believed to have the following function(s):
Involved in neutrophil activation. The sequence for protein Small
inducible cytokine B5 precursor (SEQ ID NO:190) is given at the end
of the application, as "Small inducible cytokine B5 precursor (SEQ
ID NO:190) amino acid sequence". Protein Small inducible cytokine
B5 precursor (SEQ ID NO:190) localization is believed to be
Secreted.
[1331] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: chemotaxis; signal
transduction; cell-cell signaling; positive control of cell
proliferation, which are annotation(s) related to Biological
Process; and chemokine, which are annotation(s) related to
Molecular Function.
[1332] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1333] Cluster HSENA78 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 22 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1334] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 22 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors and lung
malignant tumors. TABLE-US-00391 TABLE 4 Normal tissue distribution
Name of Tissue Number Colon 0 epithelial 2 general 38 kidney 0 Lung
3 Breast 8 Skin 0 stomach 36 Uterus 4
[1335] TABLE-US-00392 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 colon 2.6e-01
3.3e-01 1.7e-01 2.7 2.7e-01 2.2 epithelial 2.5e-01 9.0e-02 3.2e-03
4.1 8.5e-07 5.5 general 8.4e-01 7.2e-01 1 0.3 1 0.4 kidney 1
7.2e-01 1 1.0 1.7e-01 1.9 lung 8.5e-01 4.8e-01 4.1e-01 1.9 4.0e-05
3.8 breast 9.5e-01 8.7e-01 1 0.8 6.8e-01 1.2 skin 2.9e-01 4.7e-01
1.4e-01 7.0 6.4e-01 1.6 stomach 5.0e-01 4.3e-01 7.5e-01 1.0 4.3e-01
1.3 uterus 7.1e-01 8.5e-01 6.6e-01 1.3 8.0e-01 1.0
[1336] As noted above, cluster HSENA78 features 1 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Small inducible cytokine
B5 precursor (SEQ ID NO:190). A description of each variant protein
according to the present invention is now provided.
[1337] Variant protein HSENA78_P2 (SEQ ID NO:191) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSENA78_T5 (SEQ ID
NO:182). An alignment is given to the known protein (Small
inducible cytokine B5 precursor (SEQ ID NO:190)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1338] Comparison report between HSENA78_P2 (SEQ ID NO:191) and
SZ05_HUMAN (SEQ ID NO:190):
[1339] 1. An isolated chimeric polypeptide encoding for HSENA78_P2
(SEQ ID NO:191), comprising a first amino acid sequence being at
least 90% homologous to
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCVCLQTTQGVHP
KMISNLQVFAIGPQCSKVEVV corresponding to amino acids 1-81 of
SZ05_HUMAN (SEQ ID NO:190), which also corresponds to amino acids
1-81 of HSENA78_P2 (SEQ ID NO:191).
[1340] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1341] Variant protein HSENA78_P2 (SEQ ID NO:191) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 6, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSENA78_P2 (SEQ ID NO:191)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00393 TABLE 6
Amino acid mutations SNP position(s) on Alternative Previously
known amino acid sequence amino acid(s) SNP? 80 V->No 81
V->No
[1342] Variant protein HSENA78_P2 (SEQ ID NO:191) is encoded by the
following transcript(s): HSENA78_T5 (SEQ ID NO:182), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript HSENA78_T5 (SEQ ID NO:182) is shown in bold;
this coding portion starts at position 149 and ends at position
391. The transcript also has the following SNPs as listed in Table
7 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSENA78_P2 (SEQ ID NO:191) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00394 TABLE 7 Nucleic acid SNPs
SNP position on nucleotide Alternative Previously known sequence
nucleic acid SNP? 92 C->T Yes 144 C->T No 1151 A->T Yes
1389 T->C No 1867 C->G Yes 145 C->T No 181 C->T Yes 316
G->A Yes 388 G->No 390 T->No 605 T->No 972 C->T Yes
1105 A->G Yes
[1343] As noted above, cluster HSENA78 features 7 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1344] Segment cluster HSENA78_node.sub.--0 (SEQ ID NO:183)
according to the present invention is supported by 24 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSENA78_T5 (SEQ ID NO: 182). Table 8 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00395 TABLE 8 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSENA78_T5 1 257 (SEQ ID NO:182)
[1345] Segment cluster HSENA78_node.sub.--2 (SEQ ID NO:184)
according to the present invention is supported by 22 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSENA78_T5 (SEQ ID NO:182). Table 9 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00396 TABLE 9 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSENA78_T5 258 390 (SEQ ID NO:182)
[1346] Segment cluster HSENA78_node.sub.--6 (SEQ ID NO:185)
according to the present invention is supported by 68 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSENA78_T5 (SEQ ID NO: 182). Table 10 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00397 TABLE 10 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSENA78_T5 585 2370 (SEQ ID NO:182)
[1347] Segment cluster HSENA78_node.sub.--9 (SEQ ID NO:186)
according to the present invention is supported by 28 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSENA78_T5 (SEQ ID NO:182). Table 11 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00398 TABLE 11 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSENA78_T5 2394 2546 (SEQ ID NO:182)
[1348] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1349] Segment cluster HSENA78_node.sub.--3 (SEQ ID NO:187)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HSENA78_T5
(SEQ ID NO: 182). Table 12 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00399 TABLE
12 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSENA78_T5 391 500 (SEQ ID
NO:182)
[1350] Segment cluster HSENA78_node.sub.--4 (SEQ ID NO:188)
according to the present invention is supported by 17 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): HSENA78
T5 (SEQ ID NO: 182). Table 13 below describes the starting and
ending position of this segment on each transcript. TABLE-US-00400
TABLE 13 Segment location on transcripts Segment Segment Transcript
name starting position ending position HSENA78_T5 501 584 (SEQ ID
NO:182)
[1351] Segment cluster HSENA78_node.sub.--8 (SEQ ID NO:189)
according to the present invention can be found in the following
transcript(s): HSENA78_T5 (SEQ ID NO:182). Table 14 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00401 TABLE 14 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HSENA78_T5 2371 2393 (SEQ ID NO:182)
[1352] Microarray (chip) data is also available for this gene as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 15. TABLE-US-00402 TABLE
15 Oligonucleotides related to this gene Overexpressed
Oligonucleotide name in cancers Chip reference
HSENA78.sub.--0.sub.--1.sub.--0 breast cancer Breast (SEQ ID
NO:898)
[1353] Variant protein alignment to the previously known
protein:
[1354] Sequence name: /tmp/5kiQY6MxWx/pLnTrxsCqk:SZ05_HUMAN (SEQ ID
NO:190)
[1355] Sequence Documentation:
[1356] Alignment of: HSENA78_P2 (SEQ ID NO:191).times.SZ05_HUMAN
(SEQ ID NO:190).
[1357] Alignment segment 1/1: TABLE-US-00403 Quality: 767.00
Escore: 0 Matching length: 81 Total length: 81 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00043 Alignment: TABLE-US-00404 . . . 1
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCV 50
|||||||||||||||||||||||||||||||||||||||||||||||||||| 1
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCV 50 . . . 51
CLQTTQGVHPKMISNLQVFAIGPQCSKVEVV 81 ||||||||||||||||||||||||||||||
51 CLQTTQGVHPKMISNLQVFAIGPQCSKVEVV 81
Description for Cluster T94936
[1358] Cluster T94936 features 2 transcript(s) and 12 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00405 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. T94936_PEA.sub.--1_T1192 T94936_PEA.sub.--1_T2
193
[1359] TABLE-US-00406 TABLE 2 Segments of interest Segment Name
Sequence ID No. T94936_PEA.sub.--1_node.sub.--14 194
T94936_PEA.sub.--1_node.sub.--16 195
T94936_PEA.sub.--1_node.sub.--2 196
T94936_PEA.sub.--1_node.sub.--20 197
T94936_PEA.sub.--1_node.sub.--23 198
T94936_PEA.sub.--1_node.sub.--0 199
T94936_PEA.sub.--1_node.sub.--11 200
T94936_PEA.sub.--1_node.sub.--13 201
T94936_PEA.sub.--1_node.sub.--17 202
T94936_PEA.sub.--1_node.sub.--6 203 T94936_PEA.sub.--1_node.sub.--8
204 T94936_PEA.sub.--1_node.sub.--9 205
[1360] TABLE-US-00407 TABLE 3 Proteins of interest Protein Name
Sequence ID No. T94936_PEA.sub.--1_P2 206 T94936_PEA.sub.--1_P3
207
[1361] As noted above, cluster T94936 features 2 transcript(s),
which were listed in Table 1 above. A description of each variant
protein according to the present invention is now provided.
[1362] Variant protein T94936_PEA.sub.--1_P2 (SEQ ID NO:206)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T94936_PEA.sub.--1_T1 (SEQ ID NO:192). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[1363] Comparison report between T94936_PEA.sub.--1_P2 (SEQ ID
NO:206) and Q8TD06 (SEQ ID NO:858) (SEQ ID NO:858):
[1364] 1. An isolated chimeric polypeptide encoding for
T94936_PEA.sub.--1_P2 (SEQ ID NO:206), comprising a first amino
acid sequence being at least 90% homologous to
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKS
KKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQY
VPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPL corresponding to amino acids
1-150 of Q8TD06 (SEQ ID NO:858), which also corresponds to amino
acids 1-150 of T94936_PEA.sub.--1_P2 (SEQ ID NO:206).
[1365] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1366] Variant protein T94936_PEA.sub.--1_P2 (SEQ ID NO:206) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 4, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T94936_PEA.sub.--1_P2 (SEQ ID
NO:206) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00408
TABLE 4 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 104
T->A No 28 K->R No
[1367] Variant protein T94936_PEA.sub.--1_P2 (SEQ ID NO:206) is
encoded by the following transcript(s): T94936_PEA.sub.--1_T1 (SEQ
ID NO:192), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T94936_PEA.sub.--1_T1 (SEQ ID NO:192) is shown in bold; this coding
portion starts at position 76 and ends at position 525. The
transcript also has the following SNPs as listed in Table 5 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T94936_PEA.sub.--1_P2 (SEQ ID NO:206) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00409 TABLE 5 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 158 A->G No 186 A->G No 385 A->G
No
[1368] Variant protein T94936_PEA.sub.--1_P3 (SEQ ID NO:207)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T94936_PEA.sub.--1_T2 (SEQ ID NO: 193). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[1369] Comparison report between T94936_PEA.sub.--1_P3 (SEQ ID
NO:207) and Q8TD06 (SEQ ID NO:858):
[1370] 1. An isolated chimeric polypeptide encoding for
T94936_PEA.sub.--1_P3 (SEQ ID NO:207), comprising a first amino
acid sequence being at least 90% homologous to
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKS
KKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQY VPRIMFV
corresponding to amino acids 1-122 of Q8TD06 (SEQ ID NO:858), which
also corresponds to amino acids 1-122 of T94936_PEA.sub.--1_P3 (SEQ
ID NO:207), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence GMYVISFHQIYKISRNQHSCFYF (SEQ ID NO:
1019) corresponding to amino acids 123-145 of T94936_PEA.sub.--1_P3
(SEQ ID NO:207), wherein said first and second amino acid sequences
are contiguous and in a sequential order.
[1371] 2. An isolated polypeptide encoding for a tail of
T94936_PEA.sub.--1_P3 (SEQ ID NO:207), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GMYVISFHQIYKISRNQHSCFYF (SEQ ID NO:1019) in T94936_PEA.sub.--1_P3
(SEQ ID NO:207).
[1372] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1373] Variant protein T94936_PEA.sub.--1_P3 (SEQ ID NO:207) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 6, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T94936_PEA.sub.--1_P3 (SEQ ID
NO:207) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-0041
0 TABLE 6 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 104
T->A No 28 K->R No
[1374] Variant protein T94936_PEA.sub.--1_P3 (SEQ ID NO:207) is
encoded by the following transcript(s): T94936_PEA.sub.--1_T2 (SEQ
ID NO:193), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T94936_PEA.sub.--1_T2 (SEQ ID NO:193) is shown in bold; this coding
portion starts at position 76 and ends at position 510. The
transcript also has the following SNPs as listed in Table 7 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T94936_PEA.sub.--1_P3 (SEQ ID NO:207) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00411 TABLE 7 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 158 A->G No 186 A->G No 385 A->G No
746 T->C No 889 A->C No 889 A->G No 980A->No
1006A->No 1105A->No 1356A->G No
[1375] As noted above, cluster T94936 features 12 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1376] Segment cluster T94936_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:194) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 8 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00412 TABLE 8 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T2 (SEQ ID 443 803 NO: 193)
[1377] Segment cluster T94936_PEA.sub.--1_node.sub.--16 (SEQ ID
NO:195) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 9 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00413 TABLE 9 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T2 (SEQ ID 804 1213 NO: 193)
[1378] Segment cluster T94936_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:196) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 10 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00414 TABLE 10 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 49 184 NO: 192) T94936_PEA.sub.--1_T2
(SEQ ID 49 184 NO: 193)
[1379] Segment cluster T94936_PEA.sub.--1_node.sub.--20 (SEQ ID
NO:197) according to the present invention is supported by 46
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 11
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00415 TABLE 11 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T94936_PEA.sub.--1_T2 (SEQ ID 1298 1526 NO:
193)
[1380] Segment cluster T94936_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:198) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192). Table 12
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00416 TABLE 12 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T94936_PEA.sub.--1_T1 (SEQ ID 527 751 NO: 192)
[1381] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1382] Segment cluster T94936_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:199) according to the present invention is supported by 32
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 13 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00417 TABLE 13 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 1 48 NO: 192) T94936_PEA.sub.--1_T2
(SEQ ID 1 48 NO: 193)
[1383] Segment cluster T94936_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:200) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 14 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00418 TABLE 14 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 302 378 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 302 378 NO: 193)
[1384] Segment cluster T94936_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:201) according to the present invention is supported by 50
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 15 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00419 TABLE 15 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 379 442 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 379 442 NO: 193)
[1385] Segment cluster T94936_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:202) according to the present invention is supported by 51
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 16 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00420 TABLE 16 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 443 526 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 1214 1297 NO: 193)
[1386] Segment cluster T94936_PEA .sub.--1 node.sub.--6 (SEQ ID
NO:203) according to the present invention is supported by 74
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 17 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00421 TABLE 17 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 185 248 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 185 248 NO: 193)
[1387] Segment cluster T94936_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:204) according to the present invention can be found in the
following transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO:192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 18 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00422 TABLE 18 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 249 252 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 249 252 NO: 193)
[1388] Segment cluster T94936_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:205) according to the present invention is supported by 68
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T94936_PEA.sub.--1_T1 (SEQ ID NO: 192) and
T94936_PEA.sub.--1_T2 (SEQ ID NO:193). Table 19 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00423 TABLE 19 Segment location on transcripts Segment
Segment Transcript name starting position ending position
T94936_PEA.sub.--1_T1 (SEQ ID 253 301 NO: 192)
T94936_PEA.sub.--1_T2 (SEQ ID 253 301 NO: 193)
[1389] Variant protein alignment to the previously known
protein:
[1390] Sequence name: /tmp/1R8BEXWutz/cdFRKHIcZR:Q8TD06 (SEQ ID
NO:858).
[1391] Sequence Documentation:
[1392] Alignment of: T94936_PEA.sub.--1_P2 (SEQ ID
NO:206).times.Q8TD06 (SEQ ID NO:858).
[1393] Alignment segment 1/1: TABLE-US-00424 Quality: 1486.00
Escore: 0 Matching length: 150 Total length: 150 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00044 Alignment: TABLE-US-00425 . . . 1
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEE 50 . . . 51
GLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
GLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNL 100 . . . 101
MHETTDKNLSPDGQYVPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
MHETTDKNLSPDGQYVPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPL 150
[1394] Sequence name: /tmp/AG3unO0N3y/kjgGehygST:Q8TD06 (SEQ ID
NO:858)
[1395] Sequence Documentation:
[1396] Alignment of: T94936_PEA.sub.--1_P3 (SEQ ID
NO:207).times.Q8TD06 (SEQ ID NO:858).
[1397] Alignment segment 1/1: TABLE-US-00426 Quality: 1214.00
Escore: 0 Matching length: 122 Total length: 122 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00045 Alignment: TABLE-US-00427 . . . 1
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEE 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEE 50 . . . 51
GLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
GLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNL 100..101
MHETTDKNLSPDGQYVPRIMFV 122 |||||||||||||||||||||| 101
MHETTDKNLSPDGQYVPRIMFV 122
Expression of Homo sapiens Breast Cancer Membrane Protein 11
(BCMP11) T94936 Transcripts Which are Detectable by Amplicon as
Depicted in Sequence Name T94936 seg14 (SEQ ID NO:861) in Normal
and Cancerous Breast Tissues
[1398] Expression of Homo sapiens breast cancer membrane protein 11
(BCMP11) transcripts detectable by or according to seg14, T94936
seg14 (SEQ ID NO:861) amplicon(s) and T94936 seg14F (SEQ ID NO:859)
and T94936 seg14R (SEQ ID NO:860) primers was measured by real time
PCR. In this specific example, the real-time PCR reaction
efficiency was assumed to be 2 and was not calculated by a standard
curve reaction (as detailed above in the section of "Real-Time
RT-PCR analysis"). In parallel the expression of four housekeeping
genes--PBGD (GenBank Accession No. BC019323 (SEQ ID NO:926);
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)), and G6PD
(GenBank Accession No. NM.sub.--000402 (SEQ ID NO:918);
G6PD-amplicon (SEQ ID NO:921)) was measured similarly. For each RT
sample, the expression of the above amplicon was normalized to the
geometric mean of the quantities of the housekeeping genes. The
normalized quantity of each RT sample was then divided by the
median of the quantities of the normal post-mortem (PM) samples
(Sample Nos. 56-60, 63-67, Table 1, above, "Tissue samples in
testing panel"), to obtain a value of fold up-regulation for each
sample relative to median of the normal PM samples.
[1399] FIG. 23 is a histogram showing over expression of the
above-indicated Homo sapiens breast cancer membrane protein 11
(BCMP11) transcripts in cancerous breast samples relative to the
normal samples.
[1400] As is evident from FIG. 23, the expression of Homo sapiens
breast cancer membrane protein 11 (BCMP11) transcripts detectable
by the above amplicon(s) in cancer samples was significantly higher
than in the non-cancerous samples (Sample Nos. 56-60, 63-67, Table
1, above, "Tissue samples in testing panel"). Notably an
over-expression of at least 5 fold was found in 17 out of 28
adenocarcinoma samples.
[1401] Statistical analysis was applied to verify the significance
of these results, as described below.
[1402] The P value for the difference in the expression levels of
Homo sapiens breast cancer membrane protein 11 (BCMP11) transcripts
detectable by the above amplicon(s) in breast cancer samples versus
the normal tissue samples was determined by T test as 7.94E-02.
[1403] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
6.74E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1404] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: T94936 seg14F
forward primer (SEQ ID NO:859); and T94936 seg14R reverse primer
(SEQ ID NO:860).
[1405] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: T94936 seg14 (SEQ ID NO:861). TABLE-US-00428 T94936 seg14
Forward primer: (SEQ ID NO:859) TACAAAATTAGTAGAAATCAGCATTCTTGC
T94936 seg14 Reverse primer: (SEQ ID NO:860)
TGTAGAACTAACAAGAGCTGATATTATTGGATT94936 seg14Amplicon: (SEQ ID
NO:861) TACAAAATTAGTAGAAATCAGCATTCTTGCTTTTATTTTTAAATGCTAGT
TCAAGTACTATTCTTTTTAAAGAGAAGTCATTTCTAATCCAATAATATCA
GCTCTTGTTAGTTCTACA
Description for Cluster Z41644
[1406] Cluster Z41644 features 1 transcript(s) and 21 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00429 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. Z41644_PEA.sub.--1_T5 208
[1407] TABLE-US-00430 TABLE 2 Segments of interest Segment Name
Sequence ID No. Z41644_PEA.sub.--1_node.sub.--0 209
Z41644_PEA.sub.--1_node.sub.--11 210
Z41644_PEA.sub.--1_node.sub.--12 211
Z41644_PEA.sub.--1_node.sub.--15 212
Z41644_PEA.sub.--1_node.sub.--20 213
Z41644_PEA.sub.--1_node.sub.--24 214
Z41644_PEA.sub.--1_node.sub.--1 215
Z41644_PEA.sub.--1_node.sub.--10 216
Z41644_PEA.sub.--1_node.sub.--13 217
Z41644_PEA.sub.--1_node.sub.--16 218
Z41644_PEA.sub.--1_node.sub.--17 219
Z41644_PEA.sub.--1_node.sub.--19 220
Z41644_PEA.sub.--1_node.sub.--2 221
Z41644_PEA.sub.--1_node.sub.--21 222
Z41644_PEA.sub.--1_node.sub.--22 223
Z41644_PEA.sub.--1_node.sub.--23 224
Z41644_PEA.sub.--1_node.sub.--25 225
Z41644_PEA.sub.--1_node.sub.--3 226 Z41644_PEA.sub.--1_node.sub.--4
227 Z41644_PEA.sub.--1_node.sub.--6 228
Z41644_PEA.sub.--1_node.sub.--9 229
[1408] TABLE-US-00431 TABLE 3 Proteins of interest Protein Name
Sequence ID No. Z41644_PEA.sub.--1_P10 231
[1409] These sequences are variants of the known protein Small
inducible cytokine B14 precursor (SEQ ID NO:230) (SwissProt
accession identifier SZ14_HUMAN; known also according to the
synonyms CXCL14; Chemokine BRAK), SEQ ID NO: 230, referred to
herein as the previously known protein.
[1410] Protein Small inducible cytokine B14 precursor (SEQ ID
NO:230) is known or believed to have the following function(s): Not
chemotactive for T-cells, B-cells, monocytes, natural killer cells
or ghranulocytes. Does not inhibit proliferation of myeloid
progenitors in colony formation assays. The sequence for protein
Small inducible cytokine B14 precursor (SEQ ID NO:230) is given at
the end of the application, as "Small inducible cytokine B14
precursor (SEQ ID NO:230) amino acid sequence". Protein Small
inducible cytokine B14 precursor (SEQ ID NO:230) localization is
believed to be Secreted.
[1411] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: chemotaxis; signal
transduction; cell-cell signaling, which are annotation(s) related
to Biological Process; and chemokine, which are annotation(s)
related to Molecular Function.
[1412] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from; or
Locuslink, available from ncbi.nim.nih.gov/projects/LocusLink/.
[1413] Cluster Z41644 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 24 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1414] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 24 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: lung malignant tumors, breast malignant
tumors and pancreas carcinoma. TABLE-US-00432 TABLE 4 Normal tissue
distribution Name of Tissue Number bone 45 brain 62 colon 327
epithelial 179 general 104 head and neck 10 kidney 219 lung 6 lymph
nodes 37 breast 87 bone marrow 0 muscle 20 ovary 36 Pancreas 0
prostate 78 skin 591 stomach 109 Thyroid 386 uterus 218
[1415] TABLE-US-00433 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 bone 4.9e-01
8.5e-01 1.8e-01 1.9 5.3e-01 1.0 brain 6.7e-01 8.0e-01 9.1e-01 0.6
9.9e-01 0.4 colon 6.4e-01 7.7e-01 9.7e-01 0.4 1 0.3 epithelial
4.1e-01 9.4e-01 9.6e-01 0.7 1 0.4 general 1.5e-01 9.4e-01 1.8e-01
1.0 1 0.5 head and neck 1.9e-01 3.3e-01 4.6e-01 2.8 7.5e-01 1.5
kidney 7.7e-01 8.2e-01 7.0e-01 0.7 9.5e-01 0.5 lung 2.2e-01 5.0e-01
1.3e-04 8.7 8.1e-03 4.1 lymph nodes 6.3e-01 8.7e-01 6.3e-01 1.2
9.2e-01 0.6 breast 4.0e-01 6.5e-01 3.9e-04 3.5 2.9e-02 1.9 bone
marrow 1 6.7e-01 1 1.0 5.3e-01 1.9 muscle 5.2e-01 6.1e-01 2.7e-01
3.2 6.3e-01 1.2 ovary 6.7e-01 7.1e-01 7.6e-01 1.0 8.6e-01 0.8
pancreas 2.2e-02 2.3e-02 5.7e-03 7.8 1.6e-03 8.2 prostate 8.8e-01
9.0e-01 8.3e-01 0.6 9.3e-01 0.5 skin 5.9e-01 6.9e-01 2.3e-01 0.3 1
0.0 stomach 6.1e-01 8.9e-01 8.1e-01 0.7 9.9e-01 0.4 Thyroid 7.0e-01
7.0e-01 9.9e-01 0.4 9.9e-01 0.4 uterus 5.3e-01 8.2e-01 9.5e-01 0.5
1 0.3
[1416] As noted above, cluster Z41644 features 1 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Small inducible cytokine
B14 precursor (SEQ ID NO:230). A description of each variant
protein according to the present invention is now provided.
[1417] Variant protein Z41644_PEA.sub.--1_P10 (SEQ ID NO:231)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). An alignment is given to the
known protein (Small inducible cytokine B14 precursor (SEQ ID
NO:230)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[1418] Comparison report between Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231) and SZ14_HUMAN (SEQ ID NO:230):
[1419] 1. An isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
1-95 of SZ14_HUMAN (SEQ ID NO:230), which also corresponds to amino
acids 1-95 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020)
corresponding to amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ
ID NO:231), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[1420] 2. An isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231).
[1421] Comparison report between Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231) and Q9NS21 (SEQ ID NO:862):
[1422] 1. An isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
13-107 of Q9NS21 (SEQ ID NO:862), which also corresponds to amino
acids 1-95 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020)
corresponding to amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ
ID NO:231), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[1423] 2. An isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231).
[1424] Comparison report between Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231) and AAQ89265 (SEQ ID NO:863):
[1425] 1. An isolated chimeric polypeptide encoding for
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a first amino
acid sequence being at least 90% homologous to
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVII
TTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR corresponding to amino acids
13-107 of AAQ89265, which also corresponds to amino acids 1-95 of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) corresponding to
amino acids 96-123 of Z41644_PEA.sub.--1_P10 (SEQ ID NO:231),
wherein said first and second amino acid sequences are contiguous
and in a sequential order.
[1426] 2. An isolated polypeptide encoding for a tail of
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
YAPPLLTFLPTRPSCGSQDGKGPPHQVI (SEQ ID NO:1020) in
Z41644_PEA.sub.--1_P10 (SEQ ID NO:231).
[1427] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1428] Variant protein Z41644_PEA.sub.--1_P10 (SEQ ID NO:231) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 6, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00434
TABLE 6 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 32 P->H
Yes 64 S->No 80 T->A No 80 T->P No
[1429] Variant protein Z41644_PEA.sub.--1_P10 (SEQ ID NO:231) is
encoded by the following transcript(s): Z41644_PEA.sub.--1_T5 (SEQ
ID NO:208), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
Z41644_PEA.sub.--1_T5 (SEQ ID NO:208) is shown in bold; this coding
portion starts at position 744 and ends at position 1112. The
transcript also has the following SNPs as listed in Table 7 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein Z41644_PEA.sub.--1_P10 (SEQ ID NO:231) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00435 TABLE 7 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 102 A->G Yes 572 C->No 3707 C->T Yes
3735 C->T Yes 4079 G->A No 4123 G->A Yes 4233 A->G Yes
4328 C->No 4350 A->G Yes 4376 G->A Yes 4390 A->G Yes
4619 G->T Yes 838 C->A Yes 4754 C->T No 4757 C->A No
4794 T->G No 4827 G->No 934 C->No 981 A->C No 981
A->G No 1817 A->C Yes 2546 T->No 2684 T->A No 2885
T->C Yes
[1430] As noted above, cluster Z41644 features 21 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1431] Segment cluster Z41644_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:209) according to the present invention is supported by 53
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 8 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00436 TABLE 8 Segment location on transcripts
Segment Segment starting ending Transcript name position position
Z41644_PEA.sub.--1_T5 (SEQ ID 1 616 NO: 208)
[1432] Segment cluster Z41644_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:210) according to the present invention is supported by 9
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 9 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00437 TABLE 9 Segment location on transcripts
Segment Segment starting ending Transcript name position position
Z41644_PEA.sub.--1_T5 (SEQ ID 1028 2089 NO: 208)
[1433] Segment cluster Z41644_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:211) according to the present invention is supported by 6
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 10
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00438 TABLE 10 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 2090 2350 NO:
208)
[1434] Segment cluster Z41644_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:212) according to the present invention is supported by 23
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 11
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00439 TABLE 11 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 2368 3728 NO:
208)
[1435] Segment cluster Z41644_PEA.sub.--1_node.sub.--20 (SEQ ID
NO:213) according to the present invention is supported by 260
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 12
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00440 TABLE 12 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 3938 4506 NO:
208)
[1436] Segment cluster Z41644_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:214) according to the present invention is supported by 185
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 13
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00441 TABLE 13 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 4637 4799 NO:
208)
[1437] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1438] Segment cluster Z41644_PEA.sub.--1_node.sub.--1 (SEQ ID
NO:215) according to the present invention is supported by 53
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 14
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00442 TABLE 14 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 617 697 NO:
208)
[1439] Segment cluster Z41644_PEA.sub.--1_node.sub.--10 (SEQ ID
NO:216) according to the present invention is supported by 138
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 15
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00443 TABLE 15 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 972 1027 NO:
208)
[1440] Segment cluster Z41644_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:217) according to the present invention can be found in the
following transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208).
Table 16 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00444 TABLE 16 Segment
location on transcripts Segment Segment starting ending Transcript
name position position Z41644_PEA.sub.--1_T5 (SEQ ID 2351 2367 NO:
208)
[1441] Segment cluster Z41644_PEA.sub.-1_node.sub.--16 (SEQ ID
NO:218) according to the present invention is supported by 152
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 17
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00445 TABLE 17 Segment location on
transcripts Segment ending starting position Transcript name
position Segment Z41644_PEA.sub.--1_T5 (SEQ ID 3729 3809 NO:
208)
[1442] Segment cluster Z41644_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:219) according to the present invention can be found in the
following transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208).
Table 18 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00446 TABLE 18 Segment
location on transcripts Segment Segment starting ending Transcript
name position position Z41644_PEA.sub.--1_T5 (SEQ ID 3810 3829 NO:
208)
[1443] Segment cluster Z41644_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:220) according to the present invention is supported by 112
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 19
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00447 TABLE 19 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 3830 3937 NO:
208)
[1444] Segment cluster Z41644_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:221) according to the present invention is supported by 58
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 20
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00448 TABLE 20 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 698 737 NO:
208)
[1445] Segment cluster Z41644_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:222) according to the present invention can be found in the
following transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208).
Table 21 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00449 TABLE 21 Segment
location on transcripts Segment Segment starting ending Transcript
name position position Z41644_PEA.sub.--1_T5 (SEQ ID 4507 4529 NO:
208)
[1446] Segment cluster Z41644_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:223) according to the present invention is supported by 164
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 22
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00450 TABLE 22 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 4530 4582 NO:
208)
[1447] Segment cluster Z41644_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:224) according to the present invention is supported by 169
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 23
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00451 TABLE 23 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 4583 4636 NO:
208)
[1448] Segment cluster Z41644_PEA.sub.--1_node.sub.--25 (SEQ ID
NO:225) according to the present invention is supported by 138
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 24
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00452 TABLE 24 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 4800 4902 NO:
208)
[1449] Segment cluster Z41644_PEA.sub.--1_node.sub.--3 (SEQ ID
NO:226) according to the present invention is supported by 75
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 25
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00453 TABLE 25 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 738 773 NO:
208)
[1450] Segment cluster Z41644_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:227) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 26
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00454 TABLE 26 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 774 807 NO:
208)
[1451] Segment cluster Z41644_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:228) according to the present invention is supported by 101
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 27
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00455 TABLE 27 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1 T5 (SEQ ID 808 913 NO:
208)
[1452] Segment cluster Z41644_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:229) according to the present invention is supported by 134
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): Z41644_PEA.sub.--1_T5 (SEQ ID NO:208). Table 28
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00456 TABLE 28 Segment location on
transcripts Segment Segment starting ending Transcript name
position position Z41644_PEA.sub.--1_T5 (SEQ ID 914 971 NO:
208)
[1453] Variant protein alignment to the previously known
protein:
[1454] Sequence name: /tmp/p5SSvhT9Xp/HQeIMsUrfm:SZ14_HUMAN (SEQ ID
NO:230)
[1455] Sequence Documentation:
[1456] Alignment of: Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231).times.SZ14_HUMAN (SEQ ID NO:230).
[1457] Alignment segment 1/1: TABLE-US-00457 Quality: 953.00
Escore: 0 Matching length: 95 Total length: 95 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00046 Alignment: TABLE-US-00458 . . . 1
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 50 . . . 51
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR 95
||||||||||||||||||||||||||||||||||||||||||||| 51
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR 95
[1458] Sequence name: /tmp/p5SSvhT9Xp/HQeIMsUrfm:Q9NS21 (SEQ ID
NO:862)
[1459] Sequence Documentation:
[1460] Alignment of: Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231).times.Q9NS21 (SEQ ID NO:862).
[1461] Alignment segment 1/1: TABLE-US-00459 Quality: 957.00
Escore: 0 Matching length: 96 Total length: 96 Matching Percent
100.00 Matching Percent Identity: 98.96 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 98.96 Gaps: 0
TABLE-US-00047 Alignment: TABLE-US-00460 . . . 1
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 13
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 62 . . . 51
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRY 96
|||||||||||||||||||||||||||||||||||||||||||||: 63
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRF 108
[1462] Sequence name: /tmp/p5SSvhT9Xp/HQeIMsUrfm:AAQ89265
[1463] Sequence Documentation:
[1464] Alignment of: Z41644_PEA.sub.--1_P10 (SEQ ID
NO:231).times.AAQ89265.
[1465] Alignment segment 1/1: TABLE-US-00461 Quality: 953.00
Escore: 0 Matching length: 95 Total length: 95 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00048 Alignment: TABLE-US-00462 . . . 1
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 13
MRLLAAALLLLLLALYTARVDGSKCKCSRKGPKIRYSDVKKLEMKPKYPH 62 . . . 51
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR 95
||||||||||||||||||||||||||||||||||||||||||||| 63
CEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRR 107
Description for Cluster M85491
[1466] Cluster M85491 features 2 transcript(s) and 11 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00463 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. M85491_PEA.sub.--1_T16 232 M85491_PEA.sub.--1_T20
233
[1467] TABLE-US-00464 TABLE 2 Segments of interest Segment Name
Sequence ID No. M85491_PEA.sub.--1_node.sub.--0 234
M85491_PEA.sub.--1_node.sub.--13 235
M85491_PEA.sub.--1_node.sub.--21 236
M85491_PEA.sub.--1_node.sub.--23 237
M85491_PEA.sub.--1_node.sub.--24 238
M85491_PEA.sub.--1_node.sub.--8 239 M85491_PEA.sub.--1_node.sub.--9
240 M85491_PEA.sub.--1_node.sub.--10 241
M85491_PEA.sub.--1_node.sub.--18 242
M85491_PEA.sub.--1_node.sub.--19 243
M85491_PEA.sub.--1_node.sub.--6 244
[1468] TABLE-US-00465 TABLE 3 Proteins of interest Protein Name
Sequence ID No. M85491_PEA.sub.--1_P13 246 M85491_PEA.sub.--1_P14
247
[1469] These sequences are variants of the known protein Ephrin
type-B receptor 2 [precursor] (SEQ ID NO:245) (SwissProt accession
identifier EPB2_HUMAN; known also according to the synonyms EC
2.7.1.112; Tyrosine-protein kinase receptor EPH-3; DRT; Receptor
protein-tyrosine kinase HEK5; ERK), SEQ ID NO: 245, referred to
herein as the previously known protein.
[1470] Protein Ephrin type-B receptor 2 [precursor] (SEQ ID NO:245)
is known to have the following function(s): Receptor for members of
the ephrin-B family. The sequence for protein Ephrin type-B
receptor 2 [precursor] (SEQ ID NO:245) is given at the end of the
application, as "Ephrin type-B receptor 2 [precursor] (SEQ ID
NO:245) amino acid sequence". Known polymorphisms for this sequence
are as shown in Table 4. TABLE-US-00466 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 671 A->R./FTId=VAR.sub.--004162.120
MALRRLGAALLLLPLLAAVE->MWVPVLALPVCTYA923 E->K 956 L->V 958
V->L 154 G->D 476 K->KQ 495-496 Missing 532 E->D 568
R->RR 589 M->17881->F 853 S->A
[1471] Protein Ephrin type-B receptor 2 [precursor] (SEQ ID NO:245)
localization is believed to be Type I membrane protein.
[1472] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: protein amino acid
phosphorylation; transmembrane receptor protein tyrosine kinase
signaling pathway; neurogenesis, which are annotation(s) related to
Biological Process; protein tyrosine kinase; receptor;
transmembrane-ephrin receptor; ATP binding; transferase, which are
annotation(s) related to Molecular Function; and integral membrane
protein, which are annotation(s) related to Cellular Component.
[1473] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1474] Cluster M85491 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 25 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1475] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 25 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors and a mixture
of malignant tumors from different tissues. TABLE-US-00467 TABLE 5
Normal tissue distribution Name of Tissue Number Bladder 0 Bone 0
Brain 10 Colon 31 epithelial 10 general 12 Kidney 0 Liver 0 Lung 5
Breast 8 Muscle 5 Ovary 36 pancreas 10 Skin 0 stomach 0
[1476] TABLE-US-00468 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 bladder 5.4e-01
6.0e-01 3.2e-01 2.5 4.6e-01 1.9 Bone 1 2.8e-01 1 1.0 7.0e-01 1.8
Brain 3.4e-01 3.6e-01 1.2e-01 2.9 1.8e-02 2.7 Colon 3.4e-02 5.7e-02
8.2e-02 2.8 2.0e-01 2.1 epithelial 1.7e-03 3.5e-03 2.0e-03 2.8
1.1e-02 2.2 general 4.8e-04 5.2e-04 6.7e-04 2.3 1.3e-03 1.9 Kidney
4.3e-01 3.7e-01 1 1.1 7.0e-01 1.5 Liver 1 4.5e-01 1 1.0 6.9e-01 1.5
Lung 2.2e-01 2.7e-01 6.9e-02 3.6 3.4e-02 3.6 Breast 8.2e-01 7.3e-01
6.9e-01 1.2 6.8e-01 1.2 Muscle 9.2e-01 4.8e-01 1 0.8 1.5e-01 3.2
Ovary 8.5e-01 7.3e-01 9.0e-01 0.7 6.7e-01 1.0 pancreas 5.5e-01
2.0e-01 6.7e-01 1.2 3.5e-01 1.8 Skin 2.9e-01 4.7e-01 1.4e-01 7.0
6.4e-01 1.6 stomach 1.5e-01 3.2e-01 1 1.0 8.0e-01 1.3
[1477] As noted above, cluster M85491 features 2 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Ephrin type-B receptor 2
[precursor]. A description of each variant protein according to the
present invention is now provided.
[1478] Variant protein M85491_PEA.sub.--1_P13 (SEQ ID NO:246)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M85491_PEA.sub.--1_T16 (SEQ ID NO:232). An alignment is given to
the known protein (Ephrin type-B receptor 2 [precursor]) at the end
of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1479] Comparison report between M85491_PEA.sub.--1_P13 (SEQ ID
NO:246) and EPB2_HUMAN (SEQ ID NO:245):
[1480] 1. An isolated chimeric polypeptide encoding for M8549
1_PEA.sub.--1_P13 (SEQ ID NO:246), comprising a first amino acid
sequence being at least 90% homologous to
MALRRLGMLLLLPLLMVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIR
TYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYY
EADFDSATKTFPNWMENPWVKVDTIMDESFSQVDLGGRVMKINTEVRSFGPVSRSGF
YLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVMRGSCIANAEEVD
VPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPIN
SRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSG
GREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAV
NGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYEL QYYEK
corresponding to amino acids 1-476 of EPB2_HUMAN (SEQ ID NO:245),
which also corresponds to amino acids 1-476 of
M85491_PEA.sub.--1_P13 (SEQ ID NO:246), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VPIGWVLSPSPTSLRAPLPG (SEQ ID NO:964) corresponding to amino acids
477-496 of M85491_PEA.sub.--1_P13 (SEQ ID NO:246), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[1481] 2. An isolated polypeptide encoding for a tail of
M85491_PEA.sub.--1P 13 (SEQ ID NO:246), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VPIGWVLSPSPTSLRAPLPG (SEQ ID NO:964) in M85491_PEA.sub.--1_P13 (SEQ
ID NO:246).
[1482] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region. Variant protein
M85491_PEA.sub.--1_P13 (SEQ ID NO:246) is encoded by the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232), for which
the sequence(s) is/are given at the end of the application. The
coding portion of transcript M85491_PEA.sub.--1_T16 (SEQ ID NO:232)
is shown in bold; this coding portion starts at position 143 and
ends at position 1630. The transcript also has the following SNPs
as listed in Table 7 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M85491_PEA.sub.--1_P13 (SEQ ID
NO:246) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00469
TABLE 7 Nucleic acid SNPs SNP position on Alternative Previously
nucleotide sequence nucleic acid known SNP? 799 G->A Yes 1066
C->T Yes 1519 A->G Yes 1872 C->T Yes 2044 T->C Yes 2156
G->A Yes 2606 C->A Yes 2637 G->C Yes
[1483] Variant protein M85491_PEA.sub.--1_P14 (SEQ ID NO:247)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). An alignment is given to
the known protein (Ephrin type-B receptor 2 [precursor]) at the end
of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1484] Comparison report between M85491_PEA.sub.--1_P14 (SEQ ID
NO:247) and EPB2_HUMAN (SEQ ID NO:245):
[1485] 1. An isolated chimeric polypeptide encoding for
M85491_PEA.sub.--1_P14 (SEQ ID NO:247), comprising a first amino
acid sequence being at least 90% homologous to
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIR
TYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYY
EADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGF
YLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVMRGSCIANAEEVD
VPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR corresponding to amino acids
1-270 of EPB2_HUMAN (SEQ ID NO:245), which also corresponds to
amino acids 1-270 of M85491_PEA.sub.--1_P14 (SEQ ID NO:247), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence ERQDLTMLSRLVLNSWPQMILPPQPPKVLEL (SEQ ID NO:965)
corresponding to amino acids 271-301 of M85491_PEA.sub.--1_P14 (SEQ
ID NO:247), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[1486] 2. An isolated polypeptide encoding for a tail of
M85491_PEA.sub.--1_P14 (SEQ ID NO:247), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ERQDLTMLSRLVLNSWPQMILPPQPPKVLEL (SEQ ID NO:965) in
M85491_PEA.sub.--1_P14 (SEQ ID NO:247).
[1487] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1488] Variant protein M85491_PEA.sub.--1_P14 (SEQ ID NO:247) is
encoded by the following transcript(s): M85491_PEA.sub.--1_T20 (SEQ
ID NO:233), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M85491_PEA.sub.--1_T20 (SEQ ID NO:233) is shown in bold; this
coding portion starts at position 143 and ends at position 1045.
The transcript also has the following SNPs as listed in Table 8
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M85491_PEA.sub.--1_P14 (SEQ ID NO:247) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00470-TABLE 8 Nucleic
acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 799 G->A Yes 1135 T->C Yes
1160 T->C Yes 1172 A->C Yes 1176 T->A Yes
[1489] As noted above, cluster M85491 features 11 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1490] Segment cluster M85491_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:234) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232) and
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 9 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00471 TABLE 9 Segment location on transcripts Segment
Segment Transcript name starting position ending position
M85491_PEA.sub.--1_T16 (SEQ 1 203 ID NO: 232)
M85491_PEA.sub.--1_T20 (SEQ 1 203 ID NO: 233)
[1491] Segment cluster M85491_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:235) according to the present invention is supported by 6
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 10
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00472 TABLE 10 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T20 (SEQ 954 1182 ID NO:
233)
[1492] Segment cluster M85491_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:236) according to the present invention is supported by 18
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232). Table 12
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00473 TABLE 12 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T16 (SEQ 1110 1445 ID NO:
232)
[1493] Segment cluster M85491_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:237) according to the present invention is supported by 18
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232). Table 13
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00474 TABLE 13 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T16 (SEQ 1446 1570 ID NO:
232)
[1494] Segment cluster M85491_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:238) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232). Table 14
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00475 TABLE 14 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T16 (SEQ 1571 2875 ID NO:
232)
[1495] Segment cluster M85491_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:239) according to the present invention is supported by 25
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232) and
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 15 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00476 TABLE 15 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M85491_PEA.sub.--1_T16 (SEQ 269 672 ID NO: 232)
M85491_PEA.sub.--1_T20 (SEQ 269 672 ID NO: 233)
[1496] Segment cluster M85491_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:240) according to the present invention is supported by 20
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232) and
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 17 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00477 TABLE 17 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M85491_PEA.sub.--1_T16 (SEQ 673 856 ID NO: 232)
M85491_PEA.sub.--1_T20 (SEQ 673 856 ID NO: 233)
[1497] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1498] Segment cluster M85491_PEA.sub.--1_node.sub.--10 (SEQ ID
NO:241) according to the present invention is supported by 17
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232) and
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 18 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00478 TABLE 18 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M85491_PEA.sub.--1_T16 (SEQ 857 953 ID NO: 232)
M85491_PEA.sub.--1_T20 (SEQ 857 953 ID NO: 233)
[1499] Segment cluster M85491_PEA.sub.--1_node.sub.--18 (SEQ ID
NO:242) according to the present invention is supported by 15
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232). Table 19
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00479 TABLE 19 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T16 (SEQ 954 1044 ID NO:
232)
[1500] Segment cluster M85491_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:243) according to the present invention is supported by 15
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232). Table 20
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00480 TABLE 20 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M85491_PEA.sub.--1_T16 (SEQ 1045 1109 ID NO:
232)
[1501] Segment cluster M85491_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:244) according to the present invention is supported by 11
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M85491_PEA.sub.--1_T16 (SEQ ID NO:232) and
M85491_PEA.sub.--1_T20 (SEQ ID NO:233). Table 21 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00481 TABLE 21 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M85491_PEA.sub.--1_T16 (SEQ 204 268 ID NO: 232)
M85491_PEA.sub.--1_T20 (SEQ 204 268 ID NO: 233)
[1502] Variant protein alignment to the previously known
protein:
[1503] Sequence name: /tmp/qfmsU9VtxS/DylcLC9j8v:EPB2_HUMAN (SEQ ID
NO:245)
[1504] Sequence Documentation:
[1505] Alignment of: M85491_PEA.sub.--1_P13 (SEQ ID
NO:246).times.EPB2_HUMAN (SEQ ID NO:245).
[1506] Alignment segment 1/1: TABLE-US-00482 Quality: 4726.00
Escore: 0 Matching length: 476 Total length: 476 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00049 Alignment: TABLE-US-00483 . . . 1
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYD 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYD 50 . . . 51
ENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSI 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
ENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSI 100 . . . 101
PSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQV 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
PSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQV 150 . . . 151
DLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRI 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
DLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRI 200 . . . 201
IQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVP 250 . . . 251
IGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
IGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGA 300 . . . 301
TNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDS 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
TNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDS 350 . . . 351
GGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLA 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
GGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLA 400 . . . 401
HTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
HTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD 450 . . . 451
SITLSWSQPDQPNGVILDYELQYYEK 476 |||||||||||||||||||||||||| 451
SITLSWSQPDQPNGVILDYELQYYEK 476
[1507] Sequence name: /tmp/rmnzuDbot6/GiHbjeU8iR:EPB2_HUMAN (SEQ ID
NO:245)
[1508] Sequence Documentation:
[1509] Alignment of: M85491_PEA.sub.--1_P14 (SEQ ID
NO:247).times.EPB2_HUMAN (SEQ ID NO:245).
[1510] Alignment segment 1/1: TABLE-US-00484 Quality: 2673.00
Escore: 0 Matching length: 270 Total length: 270 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00050 Alignment: TABLE-US-00485 . . . 1
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYD 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYD 50 . . . 51
ENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSI 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
ENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSI 100 . . . 101
PSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQV 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
PSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQV 150 . . . 151
DLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRI 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
DLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRI 200 . . . 201
IQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVP 250 . . . 251
IGRCMCKAGFEAVENGTVCR 270 |||||||||||||||||||| 251
IGRCMCKAGFEAVENGTVCR 270
Expression of Ephrin Type-B Receptor 2 Precursor (EC 2.7.1.112)
(Tyrosine-Protein Kinase Receptor EPH-3) M85491 Transcripts Which
are Detectable by Amplicon as Depicted in Sequence Name M85491seg24
(SEQ ID NO:866) in Normal and Cancerous Breast Tissues
[1511] Expression of Ephrin type-B receptor 2 precursor (EC
2.7.1.112) (Tyrosine-protein kinase receptor EPH-3) transcripts
detectable by or according to seg24, M85491seg24 (SEQ ID NO:866)
amplicon and M85491seg24F (SEQ ID NO:864) M85491seg24R (SEQ ID
NO:8656) primers was measured by real time PCR. In parallel the
expression of four housekeeping genes--PBGD (GenBank Accession No.
BC01 9323 (SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID
NO:929)), HPRT1 (GenBank Accession No. NM.sub.--000194 (SEQ ID
NO:930); amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)), and G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67, Table 1, above, "Tissue samples in testing panel"),
to obtain a value of fold up-regulation for each sample relative to
median of the normal PM samples.
[1512] FIG. 26 is a histogram showing over expression of the
above-indicated Ephrin type-B receptor 2 precursor (EC 2.7.1.112)
(Tyrosine-protein kinase receptor EPH-3) transcripts in cancerous
breast samples relative to the normal samples.
[1513] As is evident from FIG. 26, the expression of Ephrin type-B
receptor 2 precursor (EC 2.7.1.112) (Tyrosine-protein kinase
receptor EPH-3) transcripts detectable by the above amplicon in a
few cancer samples was higher than in the non-cancerous samples
(Sample Nos. 56-60, 63-67, Table 1, above, "Tissue samples in
testing panel").
[1514] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: M85491seg24F
(SEQ ID NO:864) forward primer; and M85491seg24R (SEQ ID NO:865)
reverse primer.
[1515] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: M85491 seg24 (SEQ ID NO:866). TABLE-US-00486 M85491seg24
Forward primer (SEQ ID NO:864): GGCGTCTTTCTCCCTCTGMC M85491seg24
Reverse primer (SEQ ID NO:865): GTCCCATTCTGGGTGCTGTG M85491seg24
Amplicon (SEQ ID NO:866):
GGCGTCTTTCTCCCTCTGAACCTCAGTTTCCACCTGTGTCGAGTGTGGGT
GAGACCCCTCGCGGGGAGCTATGCAGGTTACGGAGAAAAGGCAGCACAGC
ACCCAGAATGGGAC
Expression of Ephrin Type-B Receptor 2 Precursor M85491
Transcripts, Which are Detectable by Amplicon as Depicted in
Sequence Name M85491 seg24 (SEQ ID NO:866) in Different Normal
Tissues
[1516] Expression of Ephrin type-B receptor 2 precursor transcripts
detectable by or according to M85491 seg24 amplicon(s) (SEQ ID
NO:866) and M85491 seg24F (SEQ ID NO:864) and M85491 seg24R (SEQ ID
NO:865) primers was measured by real time PCR. In parallel the
expression of four housekeeping genes--RPL 19 (GenBank Accession
No. NM.sub.--000981 (SEQ ID NO:934) RPL19 amplicon (SEQ ID
NO:937)), TATA box (GenBank Accession No. NM.sub.--003194 (SEQ ID
NO:938); TATA amplicon (SEQ ID NO:941)), UBC (GenBank Accession No.
BC000449 (SEQ ID NO:942); amplicon--Ubiquitin-amplicon (SEQ ID
NO:945)) and SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured
similarly. For each RT sample, the expression of the above amplicon
was normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the colon samples
(Sample Nos. 1-3 Table 2, "Tissue samples on normal panel", above),
to obtain a value of relative expression of each sample relative to
median of the colon samples. Primers and amplicon are as above.
[1517] The results are presented in FIG. 27, demonstrating the
expression of Ephrin type-B receptor 2 precursor M85491
transcripts, which are detectable by amplicon as depicted in
sequence name M85491 seg24 (SEQ ID NO:866), in different normal
tissues.
Description for Cluster HSSTROL3
[1518] Cluster HSSTROL3 features 6 transcript(s) and 16 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00487 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSSTROL3_T5 248 HSSTROL3_T8 249 HSSTROL3_T9 250
HSSTROL3_T10 251 HSSTROL3_T11 252 HSSTROL3_T12 253
[1519] TABLE-US-00488 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSSTROL3_node.sub.--6 254 HSSTROL3_node.sub.--10
255 HSSTROL3_node.sub.--13 256 HSSTROL3_node.sub.--15 257
HSSTROL3_node.sub.--19 258 HSSTROL3_node.sub.--21 259
HSSTROL3_node.sub.--24 260 HSSTROL3_node.sub.--25 261
HSSTROL3_node.sub.--26 262 HSSTROL3_node.sub.--28 263
HSSTROL3_node.sub.--29 264 HSSTROL3_node.sub.--11 265
HSSTROL3_node.sub.--17 266 HSSTROL3_node.sub.--18 267
HSSTROL3_node.sub.--20 268 HSSTROL3_node.sub.--27 269
[1520] TABLE-US-00489 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) HSSTROL3_P4 271 HSSTROL3_T5
(SEQ ID NO:248) HSSTROL3_P5 272 HSSTROL3_T8 (SEQ ID NO:249);
HSSTROL3_T9 (SEQ ID NO:250) HSSTROL3_P7 273 HSSTROL3_T10 (SEQ ID
NO:251) HSSTROL3_P8 274 HSSTROL3_T11 (SEQ ID NO:252) HSSTROL3_P9
275 HSSTROL3_T12 (SEQ ID NO:253)
[1521] These sequences are variants of the known protein
Stromelysin-3 precursor (SEQ ID NO:270) (SwissProt accession
identifier MM11_HUMAN; known also according to the synonyms EC
3.4.24.-; Matrix metalloproteinase-11; MMP-11; ST3; SL-3), SEQ ID
NO: 270, referred to herein as the previously known protein.
[1522] Protein Stromelysin-3 precursor (SEQ ID NO:270) is known or
believed to have the following function(s): May play an important
role in the progression of epithelial malignancies. The sequence
for protein Stromelysin-3 precursor (SEQ ID NO:270) is given at the
end of the application, as "Stromelysin-3 precursor (SEQ ID NO:270)
amino acid sequence".
[1523] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: proteolysis and
peptidolysis; developmental processes; morphogenesis, which are
annotation(s) related to Biological Process; stromelysin 3; calcium
binding; zinc binding; hydrolase, which are annotation(s) related
to Molecular Function; and extracellular matrix, which are
annotation(s) related to Cellular Component.
[1524] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1525] Cluster HSSTROL3 can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 28 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[1526] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 28 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: transitional cell carcinoma, epithelial
malignant tumors, a mixture of malignant tumors from different
tissues and pancreas carcinoma. TABLE-US-00490 TABLE 4 Normal
tissue distribution Name of Tissue Number Adrenal 0 Bladder 0 Brain
1 Colon 63 Epithelial 33 General 13 head and neck 101 Kidney 0 lung
11 breast 8 ovary 14 pancreas 0 prostate 2 skin 99 Thyroid 0 uterus
181
[1527] TABLE-US-00491 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 1
4.6e-01 1 1.0 5.3e-01 1.9 bladder 2.7e-01 3.4e-01 3.3e-03 4.9
2.1e-02 3.3 brain 3.5e-01 2.6e-01 1 1.7 3.3e-01 2.8 colon 7.7e-02
1.5e-01 3.1e-01 1.4 5.2e-01 1.0 epithelial 1.2e-04 1.2e-02 1.3e-06
2.7 4.6e-02 1.4 general 5.4e-09 3.1e-05 1.8e-16 5.0 3.1e-07 2.6
head and neck 4.6e-01 4.3e-01 1 0.6 9.4e-01 0.7 kidney 2.5e-01
3.5e-01 1.1e-01 4.0 2.4e-01 2.8 lung 1.8e-01 4.5e-01 1.9e-01 2.7
5.1e-01 1.4 breast 2.0e-01 3.4e-01 7.3e-02 3.3 2.5e-01 2.0 ovary
2.6e-01 3.2e-01 2.2e-02 2.0 7.0e-02 1.6 pancreas 9.5e-02 1.8e-01
1.8e-04 7.8 1.6e-03 5.5 prostate 8.2e-01 7.8e-01 4.5e-01 1.8
5.6e-01 1.5 skin 5.2e-01 5.8e-01 7.1e-01 0.8 1 0.3 Thyroid 2.9e-01
2.9e-01 1 1.1 1 1.1 uterus 4.2e-01 8.0e-01 7.5e-01 0.6 9.9e-01
0.4
[1528] As noted above, cluster HSSTROL3 features 6 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Stromelysin-3 precursor
(SEQ ID NO:270). A description of each variant protein according to
the present invention is now provided.
[1529] Variant protein HSSTROL3_P4 (SEQ ID NO:271) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSSTROL3_T5 (SEQ ID
NO:248). An alignment is given to the known protein (Stromelysin-3
precursor (SEQ ID NO:270)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[1530] Comparison report between HSSTROL3_P4 (SEQ ID NO:271) and
MM11_HUMAN (SEQ ID NO:270):
[1531] 1. An isolated chimeric polypeptide encoding for HSSTROL3_P4
(SEQ ID NO:271), comprising a first amino acid sequence being at
least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P4 (SEQ ID NO:271), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P4 (SEQ ID NO:271), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDMFEDAQGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPE
KNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADG corresponding to
amino acids 165-445 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 165-445 of HSSTROL3_P4 (SEQ ID NO:271),
and a third amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence ALGVRQLVGGGHSSRFSHLVVAGLPHACHRKSGSSSQVLCPEPSALLSVAG
(SEQ ID NO:966) corresponding to amino acids 446-496 of HSSTROL3_P4
(SEQ ID NO:271), wherein said first amino acid sequence, bridging
amino acid, second amino acid sequence and third amino acid
sequence are contiguous and in a sequential order.
[1532] 2. An isolated polypeptide encoding for a tail of
HSSTROL3_P4 (SEQ ID NO:271), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence TABLE-US-00492 (SEQ ID
NO:966) ALGVRQLVGGGHSSRFSHLVVAGLPHACHRKSGSSSQVLCPEPSALLSVA G in
(SEQ ID NO:271) HSSTROL3_P4.
[1533] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1534] Variant protein HSSTROL3_P4 (SEQ ID NO:271) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 6, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P4 (SEQ ID NO:271)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00493 TABLE 6
Amino acid mutations SNP position(s) on amino Previously known acid
sequence Alternative amino acid(s) SNP? 38 V->A Yes 104 R->P
Yes 214 A->No 323 Q->H Yes
[1535] Variant protein HSSTROL3_P4 (SEQ ID NO:271) is encoded by
the following transcript(s): HSSTROL3_T5 (SEQ ID NO:248), for which
the sequence(s) is/are given at the end of the application. The
coding portion of transcript HSSTROL3_T5 (SEQ ID NO:248) is shown
in bold; this coding portion starts at position 24 and ends at
position 1511. The transcript also has the following SNPs as listed
in Table 7 (given according to their position on the nucleotide
sequence, with the alternative nucleic acid listed; the last column
indicates whether the SNP is known or not; the presence of known
SNPs in variant protein HSSTROL3_P4 (SEQ ID NO:271) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00494 TABLE 7 Nucleic
acid SNPs SNP position on nucleotide Previously known sequence
Alternative nucleic acid SNP? 136 T->C Yes 334 G->C Yes 663
G->No 699->T No 992 G->C Yes 1528 A->G Yes 1710A->G
Yes 2251 A->G Yes 2392 C->No 2444 C->A Yes 2470 A->T
Yes 2687->G No 2696->G No 2710 C->No 2729->A No 2755
T->C No 2813 A->No 2813 A->C No 2963 A->No 2963 A->C
No 2993 T->C Yes 3140->T No
[1536] Variant protein HSSTROL3_P5 (SEQ ID NO:272) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSSTROL3_T8 (SEQ ID
NO:249) and HSSTROL3_T9 (SEQ ID NO:250). An alignment is given to
the known protein (Stromelysin-3 precursor (SEQ ID NO:270)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1537] Comparison report between HSSTROL3_P5 (SEQ ID NO:272) and
MM11_HUMAN (SEQ ID NO:270):
[1538] 1. An isolated chimeric polypeptide encoding for HSSTROL3_P5
(SEQ ID NO:272), comprising a first amino acid sequence being at
least 90% homologous to
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P5 (SEQ ID NO:272), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P5 (SEQ ID NO:272), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVMHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDAAFEDAQGHIWFFQ corresponding to amino acids 165-358 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
165-358 of HSSTROL3_P5 (SEQ ID NO:272), and a third amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ELGFPSSTGRDESLEHCRCQGLHK (SEQ ID NO:967) corresponding to amino
acids 359-382 of HSSTROL3_P5 (SEQ ID NO:272), wherein said first
amino acid sequence, bridging amino acid, second amino acid
sequence and third amino acid sequence are contiguous and in a
sequential order.
[1539] 2. An isolated polypeptide encoding for a tail of
HSSTROL3_P5 (SEQ ID NO:272), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence ELGFPSSTGRDESLEHCRCQGLHK
(SEQ ID NO:967) in HSSTROL3_P5 (SEQ ID NO:272).
[1540] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1541] Variant protein HSSTROL3_P5 (SEQ ID NO:272) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P5 (SEQ ID NO:272)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00495 TABLE 8
Amino acid mutations SNP position(s) on amino Previously known acid
sequence Alternative amino acid(s) SNP? 38 V->A Yes 104 R->P
Yes 214 A->No 323 Q->H Yes
[1542] Variant protein HSSTROL3_P5 (SEQ ID NO:272) is encoded by
the following transcript(s): HSSTROL3 T8 (SEQ ID NO:249) and
HSSTROL3 T9 (SEQ ID NO:250), for which the sequence(s) is/are given
at the end of the application.
[1543] The coding portion of transcript HSSTROL3_T8 (SEQ ID NO:249)
is shown in bold; this coding portion starts at position 24 and
ends at position 1169. The transcript also has the following SNPs
as listed in Table 9 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P5 (SEQ ID NO:272)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00496 TABLE 9
Nucleic acid SNPs SNP position on nucleotide Alternative Previously
known sequence nucleic acid SNP? 136 T->C Yes 334 G->C Yes
663 G->No 699->T No 992 G->C Yes 1903 C->No 1955
C->A Yes 1981 A->T Yes 2198->G No 2207->G No 2221
C->No 2240->A No 2266 T->C No 2324 A->No 2324 A->C
No 2474 A->No 2474 A->C No 2504 T->C Yes 2651->T No
[1544] The coding portion of transcript HSSTROL3_T9 (SEQ ID NO:250)
is shown in bold; this coding portion starts at position 24 and
ends at position 1169. The transcript also has the following SNPs
as listed in Table 10 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P5 (SEQ ID NO:272)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00497 TABLE
10 Nucleic acid SNPs SNP position on nucleotide Alternative
Previously known sequence nucleic acid SNP? 136 T->C Yes 334
G->C Yes 663 G->No 699->T No 992 G->C Yes 1666 A->G
Yes 1848A->G Yes 2389 A->G Yes 2530 C->No 2582 C->A Yes
2608 A->T Yes 2825->G No 2834->G No 2848 C->No
2867->A No 2893 T->C No 2951 A->No 2951 A->C No 3101
A->No 3101 A->C No 3131 T->C Yes 3278->T No
[1545] Variant protein HSSTROL3_P7 (SEQ ID NO:273) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSSTROL3_T10 (SEQ
ID NO:251). An alignment is given to the known protein
(Stromelysin-3 precursor (SEQ ID NO:270)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1546] Comparison report between HSSTROL3_P7 (SEQ ID NO:273) and
MM11_HUMAN (SEQ ID NO:270):
[1547] 1. An isolated chimeric polypeptide encoding for HSSTROL3_P7
(SEQ ID NO:273), comprising a first amino acid sequence being at
least 90% homologous to
MAPMWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P7 (SEQ ID NO:273), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P7 (SEQ ID NO:273), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDAAFEDAQGHIWFFQG corresponding to amino acids 165-359 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
165-359 of HSSTROL3_P7 (SEQ ID NO:273), and a third amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TTGVSTPAPGV (SEQ ID NO:968) corresponding to amino acids 360-370 of
HSSTROL3_P7 (SEQ ID NO:273), wherein said first amino acid
sequence, bridging amino acid, second amino acid sequence and third
amino acid sequence are contiguous and in a sequential order.
[1548] 2. An isolated polypeptide encoding for a tail of
HSSTROL3_P7 (SEQ ID NO:273), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence TTGVSTPAPGV (SEQ ID
NO:968) in HSSTROL3_P7 (SEQ ID NO:273).
[1549] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1550] Variant protein HSSTROL3_P7 (SEQ ID NO:273) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P7 (SEQ ID NO:273)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00498 TABLE
11 Amino acid mutations SNP position(s) on Alternative Previously
known amino acid sequence amino acid(s) SNP? 38 V->A Yes 104
R->P Yes 214 A->No 323 Q->H Yes
[1551] Variant protein HSSTROL3_P7 (SEQ ID NO:273) is encoded by
the following transcript(s): HSSTROL3_T10 (SEQ ID NO:251), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HSSTROL3_T10 (SEQ ID NO:251) is
shown in bold; this coding portion starts at position 24 and ends
at position 1133. The transcript also has the following SNPs as
listed in Table 12 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P7 (SEQ ID NO:273)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00499 TABLE
12 Nucleic acid SNPs SNP position on nucleotide Alternative
Previously known sequence nucleic acid SNP? 136 T->C Yes 334
G->C Yes 663 G->No 699->T No 992 G->C Yes 1386 A->G
Yes 1568 A->G Yes 2109 A->G Yes 2250 C->No 2302 C->A
Yes 2328 A->T Yes 2545->G No 2554->G No 2568 C->No
2587->A No 2613 T->C No 2671 A->No 2671 A->C No 2821
A->No 2821 A->C No 2851 T->C Yes 2998->T No
[1552] Variant protein HSSTROL3_P8 (SEQ ID NO:274) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSSTROL3_T11 (SEQ
ID NO:252). An alignment is given to the known protein
(Stromelysin-3 precursor (SEQ ID NO:270)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1553] Comparison report between HSSTROL3_P8 (SEQ ID NO:274) and
MM11_HUMAN (SEQ ID NO:270):
[1554] 1. An isolated chimeric polypeptide encoding for HSSTROL3_P8
(SEQ ID NO:274), comprising a first amino acid sequence being at
least 90% homologous to
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVLSGGRWEKTDLTYRILRFP
WQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding to
amino acids 1-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 1-163 of HSSTROL3_P8 (SEQ ID NO:274), a
bridging amino acid H corresponding to amino acid 164 of
HSSTROL3_P8 (SEQ ID NO:274), a second amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN EIAPLE
corresponding to amino acids 165-286 of MM11_HUMAN (SEQ ID NO:270),
which also corresponds to amino acids 165-286 of HSSTROL3_P8 (SEQ
ID NO:274), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence VRPCLPVPLLLCWPL (SEQ ID NO:969)
corresponding to amino acids 287-301 of HSSTROL3_P8 (SEQ ID
NO:274), wherein said first amino acid sequence, bridging amino
acid, second amino acid sequence and third amino acid sequence are
contiguous and in a sequential order.
[1555] 2. An isolated polypeptide encoding for a tail of
HSSTROL3_P8 (SEQ ID NO:274), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence VRPCLPVPLLLCWPL (SEQ ID
NO:969) in HSSTROL3_P8 (SEQ ID NO:274).
[1556] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1557] Variant protein HSSTROL3_P8 (SEQ ID NO:274) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P8 (SEQ ID NO:274)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00500 TABLE
13 Amino acid mutations SNP position(s) on Alternative Previously
known amino acid sequence amino acid(s) SNP? 38 V->A Yes 104
R->P Yes 214 A->No
[1558] Variant protein HSSTROL3_P8 (SEQ ID NO:274) is encoded by
the following transcript(s): HSSTROL3_T11 (SEQ ID NO:252), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HSSTROL3_T11 (SEQ ID NO:252) is
shown in bold; this coding portion starts at position 24 and ends
at position 926. The transcript also has the following SNPs as
listed in Table 14 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P8 (SEQ ID NO:274)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00501 TABLE
14 Nucleic acid SNPs SNP position on nucleotide Alternative
Previously known sequence nucleic acid SNP? 136 T->C Yes 334
G->C Yes 663 G->No 699->T No 935 G->A Yes 948 G->A
Yes 1084 G->C Yes 1557 C->No 1609 C->A Yes 1635 A->T
Yes 1852->G No 1861->G No 1875 C->No 1894->A No 1920
T->C No 1978A->No 1978A->C No 2128A->No 2128A->C No
2158 T->C Yes 2305->T No
[1559] Variant protein HSSTROL3_P9 (SEQ ID NO:275) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) HSSTROL3_T12 (SEQ
ID NO:253). An alignment is given to the known protein
(Stromelysin-3 precursor (SEQ ID NO:270)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1560] Comparison report between HSSTROL3_P9 (SEQ ID NO:275) and
MM11_HUMAN (SEQ ID NO:270):
[1561] 1. An isolated chimeric polypeptide encoding for HSSTROL3_P9
(SEQ ID NO:275), comprising a first amino acid sequence being at
least 90% homologous to
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQPWHAALPSS
PAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQK corresponding to amino acids
1-96 of MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino
acids 1-96 of HSSTROL3_P9 (SEQ ID NO:275), a second amino acid
sequence being at least 90% homologous to
RILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYW corresponding
to amino acids 113-163 of MM11_HUMAN (SEQ ID NO:270), which also
corresponds to amino acids 97-147 of HSSTROL3_P9 (SEQ ID NO:275), a
bridging amino acid H corresponding to amino acid 148 of
HSSTROL3_P9 (SEQ ID NO:275), a third amino acid sequence being at
least 90% homologous to
GDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVMHEFGHVLG
LQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTN
EIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGL
PSPVDAAFEDAQGHIWFFQG corresponding to amino acids 165-359 of
MM11_HUMAN (SEQ ID NO:270), which also corresponds to amino acids
149-343 of HSSTROL3_P9 (SEQ ID NO:275), and a fourth amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TTGVSTPAPGV (SEQ ID NO:968) corresponding to amino acids 344-354 of
HSSTROL3_P9 (SEQ ID NO:275), wherein said first amino acid
sequence, second amino acid sequence, bridging amino acid, third
amino acid sequence and fourth amino acid sequence are contiguous
and in a sequential order.
[1562] 2. An isolated chimeric polypeptide encoding for an edge
portion of HSSTROL3_P9 (SEQ ID NO:275), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise KR, having a structure as follows: a sequence
starting from any of amino acid numbers 96-x to 96; and ending at
any of amino acid numbers 97+((n-2)-x), in which x varies from 0 to
n-2.
[1563] 3. An isolated polypeptide encoding for a tail of
HSSTROL3_P9 (SEQ ID NO:275), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence TTGVSTPAPGV (SEQ ID
NO:968) in HSSTROL3_P9 (SEQ ID NO:275).
[1564] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1565] Variant protein HSSTROL3_P9 (SEQ ID NO:275) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 15, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P9 (SEQ ID NO:275)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00502 TABLE
15 Amino acid mutations SNP position(s) on Alternative Previously
known amino acid sequence amino acid(s) SNP? 38 V->A Yes 198
A->No 307 Q->H Yes
[1566] Variant protein HSSTROL3_P9 (SEQ ID NO:275) is encoded by
the following transcript(s): HSSTROL3_T1 2 (SEQ ID NO:253), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HSSTROL3_T1 2 (SEQ ID NO:253) is
shown in bold; this coding portion starts at position 24 and ends
at position 1085. The transcript also has the following SNPs as
listed in Table 16 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSSTROL3_P9 (SEQ ID NO:275)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00503 TABLE
16 Nucleic acid SNPs SNP position on nucleotide Alternative
Previously known sequence nucleic acid SNP? 136 T->C Yes 615
G->No 651->T No 944 G->C Yes 1275 C->No 1327 C->A
Yes 1353 A->T Yes 1570->G No 1579->G No 1593 C->No
1612->A No 1638 T->C No 1696 A->No 1696 A->C No 1846
A->No 1846 A->C No 1876 T->C Yes 2023->T No
[1567] As noted above, cluster HSSTROL3 features 16 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1568] Segment cluster HSSTROL3_node.sub.--6 (SEQ ID NO:254)
according to the present invention is supported by 14 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 17 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00504 TABLE 17 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 1 131
HSSTROL3_T8 (SEQ ID NO:249) 1 131 HSSTROL3_T9 (SEQ ID NO:250) 1 131
HSSTROL3_T10 (SEQ ID NO:251) 1 131 HSSTROL3_T11 (SEQ ID NO:252) 1
131 HSSTROL3_T12 (SEQ ID NO:253) 1 131
[1569] Segment cluster HSSTROL3_node.sub.--10 (SEQ ID NO:255)
according to the present invention is supported by 21 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 18 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00505 TABLE 18 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 132 313
HSSTROL3_T8 (SEQ ID NO:249) 132 313 HSSTROL3_T9 (SEQ ID NO:250) 132
313 HSSTROL3_T10 (SEQ ID NO:251) 132 313 HSSTROL3_T11 (SEQ ID
NO:252) 132 313 HSSTROL3_T12 (SEQ ID NO:253) 132 313
[1570] Segment cluster HSSTROL3_node.sub.--13 (SEQ ID NO:256)
according to the present invention is supported by 36 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 19 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00506 TABLE 19 Segment
location on transcripts Segment Sequent Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 362 505
HSSTROL3_T8 (SEQ ID NO:249) 362 505 HSSTROL3_T9 (SEQ ID NO:250) 362
505 HSSTROL3_T10 (SEQ ID NO:251) 362 505 HSSTROL3_T11 (SEQ ID
NO:252) 362 505 HSSTROL3_T12 (SEQ ID NO:253) 314 457
[1571] Segment cluster HSSTROL3_node.sub.--15 (SEQ ID NO:257)
according to the present invention is supported by 47 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 20 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00507 TABLE 20 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 506 639
HSSTROL3_T8 (SEQ ID NO:249) 506 639 HSSTROL3_T9 (SEQ ID NO:250) 506
639 HSSTROL3_T10 (SEQ ID NO:251) 506 639 HSSTROL3_T11 (SEQ ID
NO:252) 506 639 HSSTROL3_T12 (SEQ ID NO:253) 458 591
[1572] Segment cluster HSSTROL3_node.sub.--19 (SEQ ID NO:258)
according to the present invention is supported by 63 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 21 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00508 TABLE 21 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 699 881
HSSTROL3_T8 (SEQ ID NO:249) 699 881 HSSTROL3_T9 (SEQ ID NO:250) 699
881 HSSTROL3_T10 (SEQ ID NO:251) 699 881 HSSTROL3_T11 (SEQ ID
NO:252) 699 881 HSSTROL3_T12 (SEQ ID NO:253) 651 833
[1573] Segment cluster HSSTROL3_node.sub.--21 (SEQ ID NO:259)
according to the present invention is supported by 61 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 22 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00509 TABLE 22 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 882 1098
HSSTROL3_T8 (SEQ ID NO:249) 882 1098 HSSTROL3_T9 (SEQ ID NO:250)
882 1098 HSSTROL3_T10 (SEQ ID NO:251) 882 1098 HSSTROL3_T11 (SEQ ID
NO:252) 974 1190 HSSTROL3_T12 (SEQ ID NO:253) 834 1050
[1574] Segment cluster HSSTROL3_node.sub.--24 (SEQ ID NO:260)
according to the present invention is supported by 7 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HSSTROL3_T8
(SEQ ID NO:249) and HSSTROL3_T9 (SEQ ID NO:250). Table 23 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00510 TABLE 23 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HSSTROL3_T8 (SEQ ID NO:249) 1099 1236 HSSTROL3_T9 (SEQ ID NO:250)
1099 1236
[1575] Segment cluster HSSTROL3_node.sub.--25 (SEQ ID NO:261)
according to the present invention is supported by 13 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T8 (SEQ ID NO:249). Table 24 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00511 TABLE 24 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSSTROL3_T8 (SEQ ID NO:249) 1237 1536
[1576] Segment cluster HSSTROL3_node.sub.--26 (SEQ ID NO:262)
according to the present invention is supported by 55 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250) and HSSTROL3_T11 (SEQ ID NO:252). Table
25 below describes the starting and ending position of this segment
on each transcript. TABLE-US-00512 TABLE 25 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSSTROL3_T5 (SEQ ID NO:248) 1099 1240 HSSTROL3_T8
(SEQ ID NO:249) 1537 1678 HSSTROL3_T9 (SEQ ID NO:250) 1237 1378
HSSTROL3_T11 (SEQ ID NO:252) 1191 1332
[1577] Segment cluster HSSTROL3_node.sub.--28 (SEQ ID NO:263)
according to the present invention is supported by 10 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T9 (SEQ ID NO:250) and
HSSTROL3_T10 (SEQ ID NO:251). Table 26 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00513 TABLE 26 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSSTROL3_T5 (SEQ ID NO:248) 1357 2283 HSSTROL3_T9 (SEQ ID NO:250)
1495 2421 HSSTROL3_T10 (SEQ ID NO:251) 1215 2141
[1578] Segment cluster HSSTROL3_node.sub.--29 (SEQ ID NO:264)
according to the present invention is supported by 109 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T11 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 27 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00514 TABLE 27 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 2284 3194
HSSTROL3_T8 (SEQ ID NO:249) 1795 2705 HSSTROL3_T9 (SEQ ID NO:250)
2422 3332 HSSTROL3_T10 (SEQ ID NO:251) 2142 3052 HSSTROL3_T11 (SEQ
ID NO:252) 1449 2359 HSSTROL3_T12 (SEQ ID NO:253) 1167 2077
[1579] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1580] Segment cluster HSSTROL3_node.sub.--11 (SEQ ID NO:265)
according to the present invention is supported by 25 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251) and
HSSTROL3_T11 (SEQ ID NO:252). Table 28 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00515 TABLE 28 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSSTROL3_T5 (SEQ ID NO:248) 314 361 HSSTROL3_T8 (SEQ ID NO:249) 314
361 HSSTROL3_T9 (SEQ ID NO:250) 314 361 HSSTROL3_T10 (SEQ ID
NO:251) 314 361 HSSTROL3_T11 (SEQ ID NO:252) 314 361
[1581] Segment cluster HSSTROL3_node.sub.--17 (SEQ ID NO:266)
according to the present invention is supported by 45 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 29 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00516 TABLE 29 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 640 680
HSSTROL3_T8 (SEQ ID NO:249) 640 680 HSSTROL3_T9 (SEQ ID NO:250) 640
680 HSSTROL3_T10 (SEQ ID NO:251) 640 680 HSSTROL3_T11 (SEQ ID
NO:252) 640 680 HSSTROL3_T12 (SEQ ID NO:253) 592 632
[1582] Segment cluster HSSTROL3_node.sub.--18 (SEQ ID NO:267)
according to the present invention can be found in the following
transcript(s): HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID
NO:249), HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 30 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00517 TABLE 30 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 681 698
HSSTROL3_T8 (SEQ ID NO:249) 681 698 HSSTROL3_T9 (SEQ ID NO:250) 681
698 HSSTROL3_T10 (SEQ ID NO:251) 681 698 HSSTROL3_T11 (SEQ ID
NO:252) 681 698 HSSTROL3_T12 (SEQ ID NO:253) 633 650
[1583] Segment cluster HSSTROL3_node.sub.--20 (SEQ ID NO:268)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HSSTROL3_T11
(SEQ ID NO:252). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00518 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSSTROL3_T11 (SEQ ID NO:252) 882
973
[1584] Segment cluster HSSTROL3_node.sub.--27 (SEQ ID NO:269)
according to the present invention is supported by 50 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSSTROL3_T5 (SEQ ID NO:248), HSSTROL3_T8 (SEQ ID NO:249),
HSSTROL3_T9 (SEQ ID NO:250), HSSTROL3_T10 (SEQ ID NO:251),
HSSTROL3_T11 (SEQ ID NO:252) and HSSTROL3_T12 (SEQ ID NO:253).
Table 32 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00519 TABLE 32 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HSSTROL3_T5 (SEQ ID NO:248) 1241 1356
HSSTROL3_T8 (SEQ ID NO:249) 1679 1794 HSSTROL3_T9 (SEQ ID NO:250)
1379 1494 HSSTROL3_T10 (SEQ ID NO:251) 1099 1214 HSSTROL3_T11 (SEQ
ID NO:252) 1333 1448 HSSTROL3_T12 (SEQ ID NO:253) 1051 1166
[1585] Variant protein alignment to the previously known
protein:
[1586] Sequence name: MM11_HUMAN (SEQ ID NO:270)
[1587] Sequence Documentation:
[1588] Alignment of: HSSTROL3_P4 (SEQ ID NO:271).times.MM11_HUMAN
(SEQ ID NO:270).
[1589] Alignment segment 1/1: TABLE-US-00520 Quality: 4444.00
Escore: 0 Matching length: 445 Total length: 445 Matching Percent
99.78 Matching Percent Identity: 99.78 Similarity: Total Percent
Similarity: 99.78 Total Percent Identity: 99.78 Gaps: 0
TABLE-US-00051 Alignment: TABLE-US-00521 . . . 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50 . . . 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100 . . . 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150 . . . 151
GRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200
||||||||||||||||||||||||||||||||||||||||||||||||| 151
GRADIMIDFARYWDGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200 . . . 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250 . . . 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300 . . . 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350 . . . 351
QGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPEKNKI 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
QGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPEKNKI 400 . . . 401
YFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADG 445
||||||||||||||||||||||||||||||||||||||||||||| 401
YFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADG 445
[1590] Sequence name: MM11_HUMAN (SEQ ID NO:270)
[1591] Sequence Documentation:
[1592] Alignment of: HSSTROL3_P5 (SEQ ID NO:272).times.MM1_HUMAN
(SEQ ID NO:270).
[1593] Alignment segment 1/1: TABLE-US-00522 Quality: 3566.00
Escore: 0 Matching length: 358 Total length: 358 Matching Percent
99.72 Matching Percent Identity: 99.72 Similarity: Total Percent
Similarity: 99.72 Total Percent Identity: 99.72 Gaps: 0
TABLE-US-00052 Alignment: TABLE-US-00523 . . . 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50 . . . 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100 . . . 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150 . . . 151
GRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200
||||||||||||||||||||||||||||||||||||||||||||||||| 151
GRADIMIDFARYWDGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200 . . . 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250 . . . 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300 . . . 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350 . . . 351
QGHIWFFQ 358 |||||||| 351 QGHIWFFQ 358
[1594] Sequence name: MM11_HUMAN (SEQ ID NO:270)
[1595] Sequence Documentation:
[1596] Alignment of: HSSTROL3_P7 (SEQ ID NO:273).times.MM11_HUMAN
(SEQ ID NO:270).
[1597] Alignment segment 1/1: TABLE-US-00524 Quality: 3575.00
Escore: 0 Matching length: 359 Total length: 359 Matching Percent
99.72 Matching Percent Identity: 99.72 Similarity: Total Percent
Similarity: 99.72 Total Percent Identity: 99.72 Gaps: 0
TABLE-US-00053 Alignment: TABLE-US-00525 . . . 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50 . . . 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100 . . . 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150 . . . 151
GRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200
||||||||||||||||||||||||||||||||||||||||||||||||| 151
GRADIMIDFARYWDGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200 . . . 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250 . . . 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300 . . . 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350 351
QGHIWFFQG 359 ||||||||| 351 QGHIWFFQG 359
[1598] Sequence name: MM11_HUMAN (SEQ ID NO:270)
[1599] Sequence Documentation:
[1600] Alignment of: HSSTROL3_P8 (SEQ ID NO:274).times.MM11_HUMAN
(SEQ ID NO:270).
[1601] Alignment segment 1/1: TABLE-US-00526 Quality: 2838.00
Escore: 0 Matching length: 286 Total length: 286 Matching Percent
99.65 Matching Percent Identity: 99.65 Similarity: Total Percent
Similarity: 99.65 Total Percent Identity: 99.65 Gaps: 0
TABLE-US-00054 Alignment: TABLE-US-00527 . . . 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50 . . . 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100 . . . 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150 . . . 151
GRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200
||||||||||||||||||||||||||||||||||||||||||||||||| 151
GRADIMIDFARYWDGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200 . . . 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250. . .251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLE 286
|||||||||||||||||||||||||||||||||||| 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLE 286
[1602] Sequence name: MM11_HUMAN (SEQ ID NO:270)
[1603] Sequence Documentation:
[1604] Alignment of: HSSTROL3_P9 (SEQ ID NO:275).times.MM11_HUMAN
(SEQ ID NO:270).
[1605] Alignment segment 1/1: TABLE-US-00528 Quality: 3316.00
Escore: 0 Matching length: 343 Total length: 359 Matching Percent
99.71 Matching Percent Identity: 99.71 Similarity: Total Percent
Similarity: 95.26 Total Percent Identity: 95.26 Gaps: 1
TABLE-US-00055 Alignment: TABLE-US-00529 . . . 1
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
MAPAAWLRSAAARALLPPMLLLLLQPPPLLARALPPDVHHLHAERRGPQP 50 . . . 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQK . . . 96
|||||||||||||||||||||||||||||||||||||||||||||| 51
WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARNRQKRFVL 100 . . . 97
............RILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 134
|||||||||||||||||||||||||||||||||||||| 101
SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHE 150 . . . 135
GRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 184
||||||||||||||||||||||||||||||||||||||||||||||||| 151
GRADIMIDFARYWDGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWT 200 . . . 185
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 234
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
IGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDC 250 . . . 235
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 284
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
RGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDA 300 . . . 285
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 334
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
VSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDA 350 335
QGHIWFFQG 343 ||||||||| 351 QGHIWFFQG 359
Expression of Stromelysin-3 Precursor (EC 3.4.24.-) (Matrix
Metalloproteinase-11) (MMP-11) (ST3) SL-3 HSSTROL3 Transcripts
Which Are Detectable by Amplicon as Depicted in Sequence Name
HSSTROL3 seg24 (SEQ ID NO:869) in Normal and Cancerous Breast
Tissues
[1606] Expression of Stromelysin-3 precursor (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) (SL-3 transcripts detectable
by or according to seg24 HSSTROL3 seg24 (SEQ ID NO:869) amplicon(s)
and HSSTROL3 seg24F (SEQ ID NO:867) and HSSTROL3 seg24R (SEQ ID
NO:868) primers was measured by real time PCR. In parallel the
expression of four housekeeping genes PBGD (GenBank Accession No.
BC01 9323 (SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID
NO:929)), HPRT1 (GenBank Accession No. NM.sub.--000194 (SEQ ID
NO:930); amplicon--HPRT1-amplicon (SEQ ID NO:933)) SDHA
(GenBankAccession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) and G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67, Table 1, above, "Tissue samples in testing panel"),
to obtain a value of fold up-regulation for each sample relative to
median of the normal PM samples.
[1607] FIG. 29A is a histogram showing over expression of the
above-indicated Stromelysin-3 precursor (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) (SL-3) transcripts in
cancerous breast samples relative to the normal samples. Values
represent the average of duplicate experiments. Error bars indicate
the minimal and maximal values obtained.
[1608] As is evident from FIG. 29A, the expression of Stromelysin-3
precursor (EC 3.4.24.-) (Matrix metalloproteinase-11) (MMP-11)
(ST3) (SL-3) transcripts detectable by the above amplicon(s) in
cancer samples was significantly higher than in the non-cancerous
samples (Sample Nos. 56-60, 63-67 Table 1, "Tissue samples in
testing panel"). Notably an over-expression of at least 5 fold was
found in 20 out of 28 adenocarcinoma samples.
[1609] Statistical analysis was applied to verify the significance
of these results, as described below.
[1610] The P value for the difference in the expression levels of
Stromelysin-3 precursor (EC 3.4.24.-) (Matrix metalloproteinase-11)
(MMP-11) (ST3) (SL-3) transcripts detectable by the above
amplicon(s) in Breast cancer samples versus the normal tissue
samples was determined by T test as 6.46E-03.
[1611] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
1.12E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results. Primer pairs
are also optionally and preferably encompassed within the present
invention; for example, for the above experiment, the following
primer pair was used as a non-limiting illustrative example only of
a suitable primer pair: HSSTROL3 seg24F forward primer (SEQ ID
NO:867); and HSSTROL3 seg24R reverse primer (SEQ ID NO:868).The
present invention also preferably encompasses any amplicon obtained
through the use of any suitable primer pair; for example, for the
above experiment, the following amplicon was obtained as a
non-limiting illustrative example only of a suitable amplicon:
HSSTROL3 seg24 (SEQ ID NO:869). TABLE-US-00530 HSSTROL3 seg24
Forward Primer (SEQ ID NO:867): ATTTCCATCCTCAACTGGCAGA HSSTROL3
seg24 Reverse Primer (SEQ ID NO:868): TGCCCTGGAACCCACG HSSTROL3
seg24 Amplicon: (SEQ ID NO:869):
ATTTCCATCCTCAACTGGCAGAGATGAGAGCCTGGAGCATTGCAGATGCC
AGGGACTTCACAAATGAAGGCACAGCATGGGAAACCTGCGTGGGTTCCAG GGCA
Expression of Stromelysin-3 Precursor (EC 3.4.24.-) (Matrix
Metalloproteinase-11) (MMP-11) (ST3) (SL-3)HSSTROL3 Transcripts
Which Are Detectable by Amplicon as Depicted in Sequence Name
HSSTROL3 seg24 (SEQ ID NO:869) in Different Normal Tissues
[1612] Expression of Stromelysin-3 precursor (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) (SL-3) transcripts detectable
by or according to HSSTROL3 seg24 (SEQ ID NO:869) amplicon(s) and
HSSTROL3 seg24F (SEQ ID NO:867) and HSSTROL3 seg24R (SEQ ID NO:868)
was measured by real time PCR. In parallel the expression of four
housekeeping genes UBC (GenBank Accession No. BC000449 (SEQ ID
NO:942); amplicon--Ubiquitin-amplicon (SEQ ID NO:945)) and SDHA
(GenBank Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)), RPL19 (GenBank Accession
No. NM.sub.--000981 (SEQ ID NO:934); RPL19 amplicon (SEQ ID
NO:937)), TATA box (GenBank Accession No. NM.sub.--003194 (SEQ ID
NO:938); TATA amplicon (SEQ ID NO:941)) was measured similarly. For
each RT sample, the expression of the above amplicon was normalized
to the geometric mean of the quantities of the housekeeping genes.
The normalized quantity of each RT sample was then divided by the
median of the quantities of the lung samples (sample Nos. 15-17
Table 2, "Tissue samples on normal panel" above), to obtain a value
of relative expression of each sample relative to median of the
lung samples. Primers and amplicon are as above.
[1613] The results are presented in FIG. 29B, demonstrating the
expression of Stromelysin-3 precursor (EC 3.4.24.-) (Matrix
metalloproteinase-11) (MMP-11) (ST3) (SL-3) HSSTROL3 transcripts,
which are detectable by amplicon as depicted in sequence name
HSSTROL3 seg24 (SEQ ID NO:869), in different normal tissues.
Expression of Stromelysin-3 Precursor (EC 3.4.24.-) (Matrix
Metalloproteinase-11) (MMP-11) (ST3) (SL-3) HSSTROL3 Transcripts
Which Are Detectable by Amplicon as Depicted in Sequence Name
HSSTROL3 junc20-21 (SEQ ID NO:872) in Normal and Cancerous Breast
Tissues
[1614] Expression of Stromelysin-3 precursor transcripts detectable
by or according to junc20-21, HSSTROL3junc20-21 (SEQ ID NO:872)
amplicon(s) and primers HSSTROL3junc20-21F (SEQ ID NO:870) and
HSSTROL3junc20-21 R (SEQ ID NO:871) was measured by real time PCR.
It should be noted that for this experiment, RNA was obtained from
Clontech (Franklin Lakes, N.J. USA 07417, clontech.com), BioChain
Inst. Inc. (Hayward, Calif. 94545 USA biochain.com), ABS
(Wilmington, Del. 19801, USA, absbioreagents.com), GOG for ovary
samples--Pediatic Cooperative Human Tissue Network, Gynecologic
Oncology Group Tissue Bank, Children Hospital of Columbus (Columbus
Ohio 43205 USA) or Ambion (Austin, Tex. 78744 USA, ambion.com).
Alternatively, RNA was generated from tissue samples using
TRI-Reagent (Molecular Research Center), according to
Manufacturer's instructions. Tissue and RNA samples were obtained
from patients or from postmortem. Total RNA samples were treated
with DNaseI (Ambion).
[1615] In parallel the expression of four housekeeping genes--PBGD
(GenBank Accession No. BC019323 (SEQ ID NO:926);
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)), G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67, Table 1: Tissue samples in testing panel, above), to
obtain a value of fold up-regulation for each sample relative to
median of the normal PM samples.
[1616] FIG. 30A is a histogram showing over expression of the
above-indicated Stromelysin-3 precursor transcripts in cancerous
breast samples relative to the normal samples.
[1617] As is evident from FIG. 30A, the expression of Stromelysin-3
precursor transcripts detectable by the above amplicon(s) in cancer
samples was significantly higher than in the non-cancerous samples
(Sample Nos. 56-60, 63-67, Table 1: Tissue samples in testing
panel, above). Notably an over-expression of at least 5 fold was
found in 13 out of 28 adenocarcinoma samples.
[1618] Statistical analysis was applied to verify the significance
of these results, as described below.
[1619] The P value for the difference in the expression levels of
Stromelysin-3 precursor transcripts detectable by the above
amplicon(s) in breast cancer samples versus the normal tissue
samples was determined by T test as 1.28E-02.
[1620] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
4.26E-02 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1621] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: HSSTROL
junc20-21 F (SEQ ID NO:870) forward primer; and HSSTROL junc20-21 R
(SEQ ID NO:871) reverse primer.
[1622] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: HSSTROL junc20-21 (SEQ ID NO:872). TABLE-US-00531 Forward
primer HSSTROL junc20-21F (SEQ ID NO:870): TCTGCTGGCCACTGTGACTG
Reverse primer HSSTROLjunc20-21R (SEQ ID NO:871):
GAAGAAAAAGAGCTCGCCTCG Amplicon HSSTROL junc20-21 (SEQ ID NO:872):
TCTGCTGGCCACTGTGACTGCAGCATATGCCCTCAGCATGTGTCCCTCTC
TCCCACCCCAGCCAGACGCCCCGCCAGATGCCTGTGAGGCCTCCTTTGAC
GCGGTCTCCACCATCCGAGGCGAGCTCTTTTTCTTC
Expression of Stromelysin-3 Precursor (EC 3.4.24.-) (Matrix
Metalloproteinase-11) (MMP-11) (ST3) (SL-3) HSSTROL3 Transcripts
Which Are Detectable by Amplicon as Depicted in Sequence Name
HSSTROL3 junc21-27 (SEQ ID NO:875) in Normal and Cancerous Breast
Tissues
[1623] Expression of Stromelysin-3 precursor transcripts detectable
by or according to junc21-27, HSSTROL3 junc21-27 (SEQ ID NO:875)
amplicon(s) and primers HSSTROL3junc21-27F (SEQ ID NO:873) and
HSSTROL3junc21-27R (SEQ ID NO:874) was measured by real time PCR
(RNA was as for the experiment above). In parallel the expression
of four housekeeping genes--PBGD (GenBank Accession No. BC019323
(SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1
(GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925)), G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID
NO:918); G6PD-amplicon (SEQ ID NO:921)) was measured similarly. For
each RT sample, the expression of the above amplicon was normalized
to the geometric mean of the quantities of the housekeeping genes.
The normalized quantity of each RT sample was then divided by the
median of the quantities of the normal post-mortem (PM) samples
(Sample Nos. 56-60, 63-67, Table 1: Tissue samples in testing
panel, above), to obtain a value of fold up-regulation for each
sample relative to median of the normal PM samples.
[1624] FIG. 30B is a histogram showing over expression of the
above-indicated Stromelysin-3 precursor transcripts in cancerous
breast samples relative to the normal samples.
[1625] As is evident from FIG. 30B, the expression of Stromelysin-3
precursor transcripts detectable by the above amplicon(s) in cancer
samples was significantly higher than in the non-cancerous samples
(Sample Nos. 56-60, 63-67 Table 1: Tissue samples in testing panel,
above). Notably an over-expression of at least 20 fold was found in
20 out of 28 adenocarcinoma samples.
[1626] Statistical analysis was applied to verify the significance
of these results, as described below.
[1627] The P value for the difference in the expression levels of
Stromelysin-3 precursor transcripts detectable by the above
amplicon(s) in breast cancer samples versus the normal tissue
samples was determined by T test as 5.98E-03.
[1628] Threshold of 20 fold overexpression was found to
differentiate between cancer and normal samples with P value of
3.66E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1629] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: HSSTROL
junc21-27F forward primer (SEQ ID NO:873); and HSSTROLjunc21-27R
reverse primer (SEQ ID NO:874).
[1630] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: HSSTROL junc21-27 (SEQ ID NO:875). TABLE-US-00532 Forward
primer HSSTROL junc21-27F (SEQ ID NO:873):
ACATTTGGTTCTTCCAAGGGACTAC Reverse primer HSSTROL junc21-27R (SEQ ID
NO:874): TCGATCTCAGAGGGCACCC Amplicon HSSTROL junc21-27 (SEQ ID
NO:875): ACATTTGGTTCTTCCAAGGGACTACTGGCGTTTCCACCCCAGCACCCGGC
GTGTAGACAGTCCCGTGCCCCGCAGGGCCACTGACTGGAGAGGGGTGCCC TCTGAGATCGA
Expression of Stromelysin-3 Precursor (EC 3.4.24.-) (Matrix
Metalloproteinase-11) (MMP-11) (ST3) (SL-3) HSSTROL3 Transcripts
Which Are Detectable by Amplicon as Depicted in Sequence Name
HSSTROL3 seg25 (SEQ ID NO:878) in Normal and Cancerous Breast
Tissues
[1631] Expression of Stromelysin-3 precursor transcripts detectable
by or according to seg25, HSSTROL3 junc21-27 (SEQ ID NO:878)
amplicon(s) and primers HSSTROL3junc21-27F (SEQ ID NO:876) and
HSSTROL3junc21-27R (SEQ ID NO:877) was measured by real time PCR
(RNA was as for the experiment above). In parallel the expression
of four housekeeping genes--PBGD (GenBank Accession No. BC019323
(SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1
(GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925)), G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID
NO:918); G6PD-amplicon (SEQ ID NO:921)) was measured similarly. For
each RT sample, the expression of the above amplicon was normalized
to the geometric mean of the quantities of the housekeeping genes.
The normalized quantity of each RT sample was then divided by the
median of the quantities of the normal post-mortem (PM) samples
(Sample Nos. 56-60, 63-67, Table 1: Tissue samples in testing
panel, above), to obtain a value of fold up-regulation for each
sample relative to median of the normal PM samples.
[1632] FIG. 30C is a histogram showing over expression of the
above-indicated Stromelysin-3 precursor transcripts in cancerous
breast samples relative to the normal samples .
[1633] As is evident from FIG. 30C, the expression of Stromelysin-3
precursor transcripts detectable by the above amplicon(s) in cancer
samples was significantly higher than in the non-cancerous samples
(Sample Nos. 56-60, 63-67 Table 1: Tissue samples in testing panel,
above). Notably an over-expression of at least 5 fold was found in
20 out of 28 adenocarcinoma samples.
[1634] Statistical analysis was applied to verify the significance
of these results, as described below.
[1635] The P value for the difference in the expression levels of
Stromelysin-3 precursor transcripts detectable by the above
amplicon(s) in breast cancer samples versus the normal tissue
samples was determined by T test as 5.79E-02.
[1636] Threshold of 5 fold overexpression was found to
differentiate between cancer and normal samples with P value of
6.75E-03 as checked by exact fisher test. The above values
demonstrate statistical significance of the results.
[1637] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: HSSTROL seg25F
forward primer (SEQ ID NO:876); and HSSTROL seg25R reverse primer
(SEQ ID NO:877).
[1638] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: HSSTROL seg25 (SEQ ID NO:878). TABLE-US-00533 Forward
primer HSSTROL seg25F (SEQ ID NO:876): CACTGCCCCAGCTTATCCC Reverse
primer HSSTROL seg25R (SEQ ID NO:877): CTCTCCCAGCCTCAGTTTCCT
Amplicon HSSTROL seg25 (SEQ ID NO:878):
CACTGCCCCAGCTTATCCCAGGCCTCCCGCTTCCCTCTGCGGGTGGGGTG
CTGAGCAGGCATTATTGGCCTGCATGTTTTACTGATGAGGAAACTGAGGC TGGGAGAG
Description for Cluster AY180924
[1639] Cluster AY180924 features 1 transcript(s) and 3 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00534 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. AY180924_PEA.sub.--1_T1276
[1640] TABLE-US-00535 TABLE 2 Segments of interest Segment Name
Sequence ID No. AY180924_PEA.sub.--1_node.sub.--3 277
AY180924_PEA.sub.--1_node.sub.--0 278
AY180924_PEA.sub.--1_node.sub.--2 279
[1641] TABLE-US-00536 TABLE 3 Proteins of interest Protein Name
Sequence ID No. AY180924_PEA.sub.--1_P3 281
[1642] These sequences are variants of the known protein Latherin
precursor (SEQ ID NO:280) (SwissProt accession identifier
LATH_HUMAN; known also according to the synonyms Breast cancer and
salivary gland expressed protein), SEQ ID NO: 280, referred to
herein as the previously known protein.
[1643] Protein Latherin precursor (SEQ ID NO:280) is known or
believed to have the following function(s): surfactant properties.
The sequence for protein Latherin precursor (SEQ ID NO:280) is
given at the end of the application, as "Latherin precursor (SEQ ID
NO:280) amino acid sequence". The protein Latherin localization is
believed to be Secreted.
[1644] As noted above, cluster AY180924 features 1 transcript,
which were listed in Table 1 above. This transcript encode for
protein which is a variant of protein Latherin precursor (SEQ ID
NO:280). A description of the variant protein according to the
present invention is now provided.
[1645] Variant protein AY180924_PEA.sub.--1_P3 (SEQ ID NO:281)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
AY180924_PEA.sub.--1_T1 (SEQ ID NO:276). An alignment is given to
the known protein (Latherin precursor (SEQ ID NO:280)) at the end
of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1646] Comparison report between AY180924_PEA.sub.--1_P3 (SEQ ID
NO:281) and LATH_HUMAN (SEQ ID NO:280):
[1647] 1. An isolated chimeric polypeptide encoding for
AY180924_PEA.sub.--1_P3 (SEQ ID NO:281), comprising a first amino
acid sequence being at least 90% homologous to
MLNVSGLFVLLCGLLVSSSAQEVLAGVSSQLLN corresponding to amino acids 1-33
of LATH_HUMAN (SEQ ID NO:280), which also corresponds to amino
acids 1-33 of AY180924_PEA.sub.--1_P3 (SEQ ID NO:281), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence GETVLLWVMQNPEPMPVKFSLAKYLGHNEHY (SEQ ID NO:971)
corresponding to amino acids 34-64 of AY180924_PEA.sub.--1_P3 (SEQ
ID NO:281), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[1648] 2. An isolated polypeptide encoding for a tail of
AY180924_PEA.sub.--1_P3 (SEQ ID NO:281) comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GETVLLWVMQNPEPMPVKFSLAKYLGHNEHY (SEQ ID NO:971) in
AY180924_PEA.sub.--1_P3(SEQ ID NO:281).
[1649] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1650] Variant protein AY180924_PEA.sub.--1_P3 (SEQ ID NO:281) is
encoded by the following transcript(s): AY180924_PEA.sub.--1_T1
(SEQ ID NO:276), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
AY180924_PEA.sub.--1_T1 (SEQ ID NO:276) is shown in bold; this
coding portion starts at position 73 and ends at position 264. The
transcript also has the following SNPs as listed in Table 4 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein AY180924_PEA.sub.--1_P3 (SEQ ID NO:281) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00537 TABLE 4 Nucleic acid SNPs
SNP position on nucleotide Alternative Previously known sequence
nucleic acid SNP? 361 C->T Yes 459 C->A Yes
[1651] As noted above, cluster AY180924 features 3 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1652] Segment cluster AY180924_PEA.sub.--1_node.sub.--3 (SEQ ID
NO:277) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AY180924_PEA.sub.--1_T1 (SEQ ID NO:276). Table 5
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00538 TABLE 5 Segment location on
transcripts Segment Segment Transcript name starting position
ending position AY180924_PEA.sub.--1_T1173 657 (SEQ ID NO: 276)
[1653] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1654] Segment cluster AY180924_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:278) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AY180924_PEA.sub.--1_T1 (SEQ ID NO:276). Table 6
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00539 TABLE 6 Segment location on
transcripts Segment Segment Transcript name starting position
ending position AY180924_PEA.sub.--1_T11 58 (SEQ ID NO: 276)
[1655] Segment cluster AY180924_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:279) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): AY180924_PEA.sub.--1_T1 (SEQ ID NO:276). Table 7
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00540 TABLE 7 Segment location on
transcripts Segment Transcript name starting position Segment
ending position AY180924_PEA.sub.--1_T159 172 (SEQ ID NO: 276)
[1656] Variant protein alignment to the previously known
protein:
[1657] Sequence name: /tmp/FepOCusBjG/YVh7Ev127H:LATH_HUMAN (SEQ ID
NO:280)
[1658] Sequence Documentation:
[1659] Alignment of: AY180924_PEA.sub.--1_P3 (SEQ ID
NO:281).times.LATH_HUMAN (SEQ ID NO:280).
[1660] Alignment segment 1/1: TABLE-US-00541 Quality: 300.00
Escore: 0 Matching length: 33 Total length: 33 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
100.00 Total Percent Identity: 100.00 Similarity: Gaps: 0
TABLE-US-00056 Alignment: TABLE-US-00542 1
MLNVSGLFVLLCGLLVSSSAQEVLAGVSSQLLN 33
||||||||||||||||||||||||||||||||| 1
MLNVSGLFVLLCGLLVSSSAQEVLAGVSSQLLN 33
Description for Cluster R75793
[1661] Cluster R75793 features 3 transcript(s) and 9 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00543 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. R75793_PEA.sub.--1_T1282 R75793_PEA.sub.--1_T3 283
R75793_PEA.sub.--1_T5 284
[1662] TABLE-US-00544 TABLE 2 Segments of interest Segment Name
Sequence ID No. R75793_PEA.sub.--1_node.sub.--0 285
R75793_PEA.sub.--1_node.sub.--9 286
R75793_PEA.sub.--1_node.sub.--11 287
R75793_PEA.sub.--1_node.sub.--14 288
R75793_PEA.sub.--1_node.sub.--4 289 R75793_PEA.sub.--1_node.sub.--5
290 R75793_PEA.sub.--1_node.sub.--6 291
R75793_PEA.sub.--1_node.sub.--8 292
R75793_PEA.sub.--1_node.sub.--13 293
[1663] TABLE-US-00545 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) R75793_PEA.sub.--1_P2 295
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) R75793_PEA.sub.--1_P5 296
R75793_PEA.sub.--1_T5 (SEQ ID NO: 284) R75793_PEA.sub.--1_P6 297
R75793_PEA.sub.--1_T3 (SEQ ID NO: 283)
[1664] Cluster R75793 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 31 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1665] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 31 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors and a mixture
of malignant tumors from different tissues. TABLE-US-00546 TABLE 4
Normal tissue distribution Name of Tissue Number epithelial 16
general 5 Breast 457
[1666] TABLE-US-00547 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 epithelial
3.3e-01 5.0e-01 9.2e-17 4.0 2.7e-07 2.0 general 1.3e-01 2.0e-01
3.4e-33 8.0 2.0e-17 3.9 Breast 5.9e-01 7.1e-01 1.2e-07 2.1 1.4e-02
1.0
[1667] As noted above, cluster R75793 features 3 transcript(s),
which were listed in Table 1 above. A description of each variant
protein according to the present invention is now provided.
[1668] Variant protein R75793_PEA.sub.--1_P2 (SEQ ID NO:295)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R75793_PEA.sub.--1_T1 (SEQ ID NO:282). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[1669] Comparison report between R75793_PEA.sub.--1_P2 (SEQ ID
NO:295) and Q96DR8 (SEQ ID NO: 294):
[1670] 1. An isolated chimeric polypeptide encoding for
R75793_PEA.sub.--1_P2 (SEQ ID NO:295), comprising a first amino
acid sequence being at least 90% homologous to
MKFLAVLVLLGVSIFLVSAQNPTTAAPADTYPATGPADDEAPDAETTAAATTATTAAPT
TATTAASTTARKDIP corresponding to amino acids 1-74 of Q96DR8 (SEQ ID
NO:294), which also corresponds to amino acids 1-74 of
R75793_PEA.sub.--1_P2 (SEQ ID NO:295), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence AP
corresponding to amino acids 75-76 of R75793_PEA.sub.--1_P2 (SEQ ID
NO:295), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[1671] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1672] Variant protein R75793_PEA.sub.--1_P2 (SEQ ID NO:295) is
encoded by the following transcript(s): R75793_PEA.sub.--1_T1 (SEQ
ID NO:282), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R75793_PEA.sub.--1_T1 (SEQ ID NO:282) is shown in bold; this coding
portion starts at position 69 and ends at position 296. The
transcript also has the following SNPs as listed in Table 6 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R75793_PEA.sub.--1_P2 (SEQ ID NO:295) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00548 TABLE 6 Nucleic acid SNPs
SNP position on Alternative Previously nucleotide sequence nucleic
acid known SNP? 15 C->A Yes 59 G->T Yes 179 T->C No 179
T->G No 227 G->A Yes 516 A->T No
[1673] Variant protein R75793_PEA.sub.--1_P5 (SEQ ID NO:296)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R75793_PEA.sub.--1_T5 (SEQ ID NO:284). The location of the variant
protein was determined according to results from a number of
different software programs and analyses, including analyses from
SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[1674] Variant protein R75793_PEA.sub.--1_P5 (SEQ ID NO:296) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R75793_PEA.sub.--1_P5 (SEQ ID
NO:296) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00549
TABLE 7 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 54H->R
Yes
[1675] Variant protein R75793_PEA.sub.--1_P5 (SEQ ID NO:296) is
encoded by the following transcript(s): R75793_PEA.sub.--1_T5 (SEQ
ID NO:284), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R75793_PEA.sub.--1_T5 (SEQ ID NO:284) is shown in bold; this coding
portion starts at position 69 and ends at position 383. The
transcript also has the following SNPs as listed in Table 8 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R75793_PEA.sub.--1_P5 (SEQ ID NO:296) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00550 TABLE 8 Nucleic acid SNPs
SNP position on Alternative Previously nucleotide sequence nucleic
acid known SNP? 15 C->A Yes 59 G->T Yes 229 A->G Yes
[1676] Variant protein R75793_PEA.sub.--1_P6 (SEQ ID NO:297)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R75793_PEA.sub.--1_T3 (SEQ ID NO:283). The location of the variant
protein was determined according to results from a number of
different software programs and analyses, including analyses from
SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[1677] Variant protein R75793_PEA.sub.--1_P6 (SEQ ID NO:297) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 9, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R75793_PEA.sub.--1 P6 (SEQ ID
NO:297) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00551
TABLE 9 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 16 R->Q
Yes
[1678] Variant protein R75793_PEA.sub.--1_P6 (SEQ ID NO:297) is
encoded by the following transcript(s): R75793_PEA.sub.--1_T3 (SEQ
ID NO:283), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R75793_PEA.sub.--1_T3 (SEQ ID NO:283) is shown in bold; this coding
portion starts at position 329 and ends at position 502. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R75793_PEA.sub.--1_P6 (SEQ ID NO:297) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00552 TABLE 10 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 327 T->C No 327 T->G No 375 G->A
Yes 635 A->T No
[1679] As noted above, cluster R75793 features 9 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1680] Segment cluster R75793_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:285) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T3 (SEQ ID NO:283). Table 11
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00553 TABLE 11 Segment location on
transcripts Segment Segment starting ending Transcript name
position position R75793_PEA.sub.--1_T3 (SEQ ID NO: 283) 1 274
[1681] Segment cluster R75793_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:286) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T5 (SEQ ID NO:284). Table 12
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00554 TABLE 12 Segment location on
transcripts Segment Segment starting ending Transcript name
position position R75793_PEA.sub.--1_T5 (SEQ ID NO: 284) 169
491
[1682] Segment cluster R75793_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:287) according to the present invention is supported by 59
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282) and
R75793_PEA.sub.--1_T3 (SEQ ID NO:283). Table 13 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00555 TABLE 13 Segment location on transcripts Segment
Segment starting ending Transcript name position position
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 169 291
R75793_PEA.sub.--1_T3 (SEQ ID NO: 283) 317 439
[1683] Segment cluster R75793_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:288) according to the present invention is supported by 41
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282) and
R75793_PEA.sub.--1_T3 (SEQ ID NO:283). Table 14 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00556 TABLE 14 Segment location on transcripts Segment
Segment starting ending Transcript name position position
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 321 527
R75793_PEA.sub.--1_T3 (SEQ ID NO: 283) 440 646
[1684] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1685] Segment cluster R75793_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:289) according to the present invention is supported by 46
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282) and
R75793_PEA.sub.--1_T5 (SEQ ID NO:284). Table 15 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00557 TABLE 15 Segment location on transcripts Segment
Segment starting ending Transcript name position position
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 1 41 R75793_PEA.sub.--1_T5
(SEQ ID NO: 284) 1 41
[1686] Segment cluster R75793_PEA.sub.--1_node.sub.--5 (SEQ ID
NO:290) according to the present invention is supported by 52
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282) and
R75793_PEA.sub.--1_T5 (SEQ ID NO:284). Table 16 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00558 TABLE 16 Segment location on transcripts Segment
Segment starting ending Transcript name position position
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 42 74 R75793_PEA.sub.--1_T5
(SEQ ID NO: 284) 42 74
[1687] Segment cluster R75793_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:291) according to the present invention is supported by 54
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282) and
R75793_PEA.sub.--1_T5 (SEQ ID NO:284). Table 17 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00559 TABLE 17 Segment location on transcripts Segment
Segment starting ending Transcript name position position
R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 75 126 R75793_PEA.sub.--1 T5
(SEQ ID NO: 284) 75 126
[1688] Segment cluster R75793_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:292) according to the present invention is supported by 57
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282),
R75793_PEA.sub.--1_T3 (SEQ ID NO:283) and R75793_PEA1_T5 (SEQ ID
NO:284). Table 18 below describes the starting and ending position
of this segment on each transcript. TABLE-US-00560 TABLE 18 Segment
location on transcripts Segment Segment starting ending Transcript
name position position R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 127
168 R75793_PEA.sub.--1_T3 (SEQ ID NO: 283) 275 316
R75793_PEA.sub.--1_T5 (SEQ ID NO: 284) 127 168
[1689] Segment cluster R75793_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:293) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R75793_PEA.sub.--1_T1 (SEQ ID NO:282). Table 19
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00561 TABLE 19 Segment location on
transcripts Segment Segment starting ending Transcript name
position position R75793_PEA.sub.--1_T1 (SEQ ID NO: 282) 292
320
[1690] Variant protein alignment to the previously known
protein:
[1691] Sequence name: Q96DR8 (SEQ ID NO:294)
[1692] Sequence Documentation:
[1693] Alignment of: R75793_PEA.sub.--1_P2 (SEQ ID
NO:295).times.Q96DR8 (SEQ ID NO:294).
[1694] Alignment segment 1/1: TABLE-US-00562 Quality: 681.00
Escore: 0 Matching length: 74 Total length: 74 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
100.00 Total Percent Identity: 100.00 Similarity: Gaps: 0
TABLE-US-00057 Alignment: TABLE-US-00563 . . . 1
MKFLAVLVLLGVSIFLVSAQNPTTAAPADTYPATGPADDEAPDAETTAAA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKFLAVLVLLGVSIFLVSAQNPTTAAPADTYPATGPADDEAPDAETTAAA 50 . . . 51
TTATTAAPTTATTAASTTARKDIP 74 |||||||||||||||||||||||| 51
TTATTAAPTTATTAASTTARKDIP 74
Expression of Homo Sapiens Small Breast Epithelial Mucin
(LOC118430) R75793Transcripts Which Are Detectable by Amplicon as
Depicted in Sequence Name R75793 junc1-13 (SEQ ID NO:881) in Normal
and Cancerous Breast Tissues
[1695] Expression of Homo sapiens small breast epithelial mucin
(LOC118430) transcripts detectable by or according to junc11-13,
R75793 junc11-13 (SEQ ID NO:881) amplicon(s) and primers R75793
junc11-13F (SEQ ID NO:879) and R75793 junc11-13R (SEQ ID NO:880)
was measured by real time PCR. In parallel the expression of four
housekeeping genes--PBGD (GenBank Accession No. BC019323 (SEQ ID
NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank
Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925)) and G6PD. (GenBank Accession No. NM.sub.--000402 (SEQ
ID NO:918); G6PD-amplicon (SEQ ID NO:921), was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the normal
post-mortem (PM) samples (Sample Nos. 56-60, 63-67, Table 1: Tissue
samples in testing panel, above), to obtain a value of fold
differential expression for each sample relative to median of the
normal PM samples.
[1696] In one experiment that was carried out no differential
expression in the cancerous samples relative to the normal PM
samples was observed. However, this may be due to a failure of this
particular experiment.
[1697] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: R75793
junc11-13F forward primer (SEQ ID NO:879); and R75793 junc11-13R
reverse primer (SEQ ID NO:880).
[1698] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: R75793 junc1-13 (SEQ ID NO:881). TABLE-US-00564 Forward
primer R75793 junc11-13F (SEQ ID NO:879): TGATGATGAAGCCCCTGATG
Reverse primer R75793 junc1-13R (SEQ ID NO:880):
TATTGTCAAGGGGCTGGAATGT Amplicon R75793junc11-13 (SEQ ID NO:881):
TGATGATGAAGCCCCTGATGCTGAAACCACTGCTGCTGCAACCACTGCGA
CCACTGCTGCTCCTACCACTGCAACCACCGCTGCTTCTACCACTGCTCGT
AAAGACATTCCAGCCCCTTGACAATA
Expression of Homo Sapiens Small Breast Epithelial Mucin
(LOC118430) R75793Transcripts Which Are Detectable by Amplicon as
Depicted in Sequence Name R75793 seg9 (SEQ ID NO:884) in Normal and
Cancerous Breast Tissues
[1699] Expression of Homo sapiens small breast epithelial mucin
(LOC118430) transcripts detectable by or according to seg9,
R75793seg9 (SEQ ID NO:884) amplicon(s) and primers R75793 seg9F
(SEQ ID NO:882) and R75793seg9R (SEQ ID NO:883) was measured by
real time PCR. In parallel the expression of four housekeeping
genes--PBGD (GenBank Accession No. BC019323 (SEQ ID NO:926);
amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank Accession
No. NM.sub.--000194 (SEQ ID NO:930); amplicon--HPRT1-amplicon (SEQ
ID NO:933)), SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID
NO:922); amplicon--SDHA-amplicon (SEQ ID NO:925)) and G6PD (GenBank
Accession No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ
ID NO:921)) was measured similarly. For each RT sample, the
expression of the above amplicon was normalized to the geometric
mean of the quantities of the housekeeping genes. The normalized
quantity of each RT sample was then divided by the median of the
quantities of the normal post-mortem (PM) samples (Sample Nos.
56-60, 63-67, Table 1: Tissue samples in testing panel, above), to
obtain a value of fold differential expression for each sample
relative to median of the normal PM samples.
[1700] In one experiment that was carried out no differential
expression in the cancerous samples relative to the normal PM
samples was observed. However, this may be due to a failure of this
particular experiment.
[1701] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: R75793seg9F
forward primer (SEQ ID NO:882); and R75793seg9R reverse primer (SEQ
ID NO:883).
[1702] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: R75793seg9 (SEQ ID NO:884). TABLE-US-00565 Forward primer
R75793seg9F (SEQ ID NO:882): TCCAGCAATAACCATTTTTCACTTC Reverse
primer R75793seg9R (SEQ ID NO:883): GCTTTCACAGACTTTTGCTTAGGATT
Amplicon R75793seg9 (SEQ ID NO:884):
TCCAGCAATAACCATTTTTCACTTCCAGCCTCATGTCAAACAGCCAGTTT
CCATGTGGATAGTCTTTGTTATAAGGAATCCTAAGCAAAAGTCTGTGAAA GC Description
for Cluster HUMCA1XIA
[1703] Cluster HUMCA1XIA features 4 transcript(s) and 46 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00566 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HUMCA1XIA_T16 298 HUMCA1XIA_T17 299 HUMCA1XIA_T19
300 HUMCA1XIA_T20 301
[1704] TABLE-US-00567 TABLE 2 Segments of interest Segment Name
Sequence ID No. HUMCA1XIA_node.sub.--0 302 HUMCA1XIA_node.sub.--2
303 HUMCA1XIA_node.sub.--4 304 HUMCA1XIA_node.sub.--6 305
HUMCA1XIA_node.sub.--8 306 HUMCA1XIA_node.sub.--9 307
HUMCA1XIA_node.sub.--18 308 HUMCA1XIA_node.sub.--54 309
HUMCA1XIA_node.sub.--55 310 HUMCA1XIA_node.sub.--92 311
HUMCA1XIA_node.sub.--11 312 HUMCA1XIA_node.sub.--15 313
HUMCA1XIA_node.sub.--19 314 HUMCA1XIA_node.sub.--21 315
HUMCA1XIA_node.sub.--23 316 HUMCA1XIA_node.sub.--25 317
HUMCA1XIA_node.sub.--27 318 HUMCA1XIA_node.sub.--29 319
HUMCA1XIA_node.sub.--31 320 HUMCA1XIA_node.sub.--33 321
HUMCA1XIA_node.sub.--35 322 HUMCA1XIA_node.sub.--37 323
HUMCA1XIA_node.sub.--39 324 HUMCA1XIA_node.sub.--41 325
HUMCA1XIA_node.sub.--43 326 HUMCA1XIA_node.sub.--45 327
HUMCA1XIA_node.sub.--47 328 HUMCA1XIA_node.sub.--49 329
HUMCA1XIA_node.sub.--51 330 HUMCA1XIA_node.sub.--57 331
HUMCA1XIA_node.sub.--59 332 HUMCA1XIA_node.sub.--62 333
HUMCA1XIA_node.sub.--64 334 HUMCA1XIA_node.sub.--66 335
HUMCA1XIA_node.sub.--68 336 HUMCA1XIA_node.sub.--70 337
HUMCA1XIA_node.sub.--72 338 HUMCA1XIA_node.sub.--74 339
HUMCA1XIA_node.sub.--76 340 HUMCA1XIA_node.sub.--78 341
HUMCA1XIA_node.sub.--81 342 HUMCA1XIA_node.sub.--83 343
HUMCA1XIA_node.sub.--85 344 HUMCA1XIA_node.sub.--87 345
HUMCA1XIA_node.sub.--89 346 HUMCA1XIA_node.sub.--91 347
[1705] TABLE-US-00568 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) HUMCA1XIA_P14 350
HUMCA1XIA_T16 (SEQ ID NO:298) HUMCA1XIA_P15 351 HUMCA1XIA_T17 (SEQ
ID NO:299) HUMCA1XIA_P16 352 HUMCA1XIA_T19 (SEQ ID NO:300)
HUMCA1XIA_P17 353 HUMCA1XIA_T20 (SEQ ID NO:301)
[1706] These sequences are variants of the known protein Collagen
alpha 1 (SEQ ID NO:348) (SwissProt accession identifier CA1B_HUMAN;
known also according to the synonyms XI), SEQ ID NO:348, referred
to herein as the previously known protein.
[1707] Protein Collagen alpha 1 (SEQ ID NO:348) is known or
believed to have the following function(s): May play an important
role in fibrillogenesis by controlling lateral growth of collagen
II fibrils. The sequence for protein Collagen alpha 1 (SEQ ID
NO:348) is given at the end of the application, as "Collagen alpha
1 (SEQ ID NO:348) amino acid sequence". Known polymorphisms for
this sequence are as shown in Table 4. TABLE-US-00569 TABLE 4 Amino
acid mutations for Known Protein SNP position(s) on amino acid
sequence Comment 625 G->V (in STL2). /FTId=VAR.sub.--013583. 676
G->R (in STL2; overlapping phenotype with Marshall
syndrome)./FTId=VAR.sub.--013584. 921-926 Missing (in STL2;
overlapping phenotype with Marshall syndrome).
/FTId=VAR.sub.--013585. 1313-1315 Missing (in STL2; overlapping
phenotype with Marshall syndrome). /FTId=VAR.sub.--013586. 1516
G->V (in STL2; overlapping phenotype with Marshall syndrome).
/FTId=VAR.sub.--013587. 941-944 KDGL->RMGC 986 Y->H 1074
R->P 1142 G->D 1218 M->W 1758 T->A 1786 S->N
[1708] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: cartilage
condensation; vision; hearing; cell-cell adhesion; extracellular
matrix organization and biogenesis, which are annotation(s) related
to Biological Process; extracellular matrix structural protein;
extracellular matrix protein, adhesive, which are annotation(s)
related to Molecular Function; and extracellular matrix; collagen;
collagen type XI, which are annotation(s) related to Cellular
Component.
[1709] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1710] Cluster HUMCA1XIA can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 32 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[1711] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 32 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: bone malignant tumors, epithelial
malignant tumors, a mixture of malignant tumors from different
tissues and lung malignant tumors. TABLE-US-00570 TABLE 5 Normal
tissue distribution Name of Tissue Number Adrenal 0 Bone 207 Brain
13 Colon 0 epithelial 11 general 11 head and neck 0 kidney 0 Lung 0
Breast 8 pancreas 0 stomach 73 Uterus 9
[1712] TABLE-US-00571 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 4.2e-01
1.9e-01 9.6e-02 3.4 8.2e-02 3.6 Bone 2.4e-01 6.3e-01 7.7e-10 4.3
5.3e-03 1.6 Brain 5.0e-01 6.9e-01 1.8e-01 2.1 4.2e-01 1.3 Colon
1.3e-02 2.9e-02 2.4e-01 3.0 3.5e-01 2.4 epithelial 3.9e-04 3.2e-03
1.3e-03 2.3 1.8e-02 1.7 general 5.6e-05 1.6e-03 9.5e-17 4.5 1.1e-09
2.8 head and neck 1.2e-01 2.1e-01 1 1.3 1 1.1 kidney 6.5e-01
7.2e-01 3.4e-01 2.4 4.9e-01 1.9 Lung 5.3e-02 9.1e-02 5.5e-05 7.3
5.0e-03 4.0 Breast 4.3e-01 5.6e-01 6.9e-01 1.4 8.2e-01 1.1 pancreas
3.3e-01 1.8e-01 4.2e-01 2.4 1.5e-01 3.7 stomach 5.0e-01 6.1e-01
6.9e-01 1.0 6.7e-01 0.8 Uterus 7.1e-01 7.0e-01 6.6e-01 1.1 6.4e-01
1.1
[1713] As noted above, cluster HUMCA1XIA features 4 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Collagen alpha 1 (SEQ ID
NO:348). A description of each variant protein according to the
present invention is now provided.
[1714] Variant protein HUMCA1XIA_P14 (SEQ ID NO:350) according to
the present invention has an amino acid sequence as given at the
end of the application; it is encoded by transcript(s)
HUMCA1XIA_T16 (SEQ ID NO:298). An alignment is given to the known
protein (Collagen alpha 1 (SEQ ID NO:348)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1715] Comparison report between HUMCA1XIA_P14 (SEQ ID NO:350) and
CA1B_HUMAN_V5 (SEQ ID NO: 349):
[1716] 1. An isolated chimeric polypeptide encoding for
HUMCA1XIA_P14 (SEQ ID NO:350), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAG
PRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQG
PIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVK
GADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPT
GDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPR
GQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGP
PGKDGLPGHPGQRGETGFQGKTGPPGPGGWGPQGPTGETGPIGERGHPGPPGPPGEQG
LPGAAGKEGAKGDPGPQGISGKDGPAGLRGFPGERGLPGAQGAPGLKGGEGPQGPPGPV
corresponding to amino acids 1-1056 of CA1B_HUMAN_V5 (SEQ ID
NO:349), which also corresponds to amino acids 1-1056 of
HUMCA1XIA_P14 (SEQ ID NO:350), and a second amino acid sequence
being at least 70%, optionally at least 80%, preferably at least
85%, more preferably at least 90% and most preferably at least 95%
homologous to a polypeptide having the sequence
VSMMIINSQTIMWNYSSSFITLML (SEQ ID NO:972) corresponding to amino
acids 1057-1081 of HUMCA1XIA_P14 (SEQ ID NO:350), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[1717] 2. An isolated polypeptide encoding for a tail of
HUMCA1XIA_P14 (SEQ ID NO:350), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence VSMMIINSQTIMWNYSSSFITLML
(SEQ ID NO:972) in HUMCA1XIA_P14 (SEQ ID NO:350).
[1718] It should be noted that the known protein sequence
(CA1B_HUMAN; SEQ ID NO:348) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CA1B_HUMAN_V5 (SEQ ID NO:349) (SEQ ID
NO:349). These changes were previously known to occur and are
listed in the table below. TABLE-US-00572 TABLE 7 Changes to
CA1B_HUMAN_V5 (SEQ ID NO: 349) SNP position(s) on amino acid
sequence Type of change 987 conflict
[1719] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1720] Variant protein HUMCA1XIA P14 (SEQ ID NO:350) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P14 (SEQ ID NO:350)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00573 TABLE 8
Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 8 W->G Yes 46
D->E Yes 559 G->S Yes 832 G>*Yes 986H->Y Yes 1061
1->M Yes 1070 V->A Yes
[1721] Variant protein HUMCA1XIA_P14 (SEQ ID NO:350) is encoded by
the following transcript(s): HUMCA1XIA_T16 (SEQ ID NO:298), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HUMCA1XIA_T16 (SEQ ID NO:298) is
shown in bold; this coding portion starts at position 319 and ends
at position 3561. The transcript also has the following SNPs as
listed in Table 9 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P14 (SEQ ID NO:350)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00574 TABLE 9
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 157 A->G No 241 T->A Yes 340
T->G Yes 456 T->G Yes 1993 G->A Yes 2812 G->T Yes 3274
C->T Yes 3282 C->T Yes 3501 A->G Yes 3527 T->C Yes
[1722] Variant protein HUMCA1XIA_P15 (SEQ ID NO:351) according to
the present invention has an amino acid sequence as given at the
end of the application; it is encoded by transcript(s)
HUMCA1XIA_T17 (SEQ ID NO:299). An alignment is given to the known
protein (Collagen alpha 1 (SEQ ID NO:348)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1723] Comparison report between HUMCA1XIA_P15 (SEQ ID NO:351) and
CA1B_HUMAN (SEQ ID NO:348):
[1724] 1. An isolated chimeric polypeptide encoding for
HUMCA1XIA_P15 (SEQ ID NO:351), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAG
PRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQG PIGPPGEK
corresponding to amino acids 1-714 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 1-714 of HUMCA1XIA_P15 (SEQ
ID NO:351), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence MCCNLSFGILIPLQK (SEQ ID NO:973)
corresponding to amino acids 715-729 of HUMCA1XIA_P15 (SEQ ID
NO:351), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[1725] 2. An isolated polypeptide encoding for a tail of
HUMCA1XIA_P15 (SEQ ID NO:351), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence MCCNLSFGILIPLQK (SEQ ID
NO:973) in HUMCA1XIA_P15 (SEQ ID NO:351).
[1726] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1727] Variant protein HUMCA1XIA_P15 (SEQ ID NO:351) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 10, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P15 (SEQ ID NO:351)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00575 TABLE
10 Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 8 W->G Yes 46
D->E Yes 559 G->S Yes
[1728] The glycosylation sites of variant protein HUMCA1XIA_P15
(SEQ ID NO:351), as compared to the known protein Collagen alpha 1
(SEQ ID NO:348), are described in Table 11 (given according to
their position(s) on the amino acid sequence in the first column;
the second column indicates whether the glycosylation site is
present in the variant protein; and the last column indicates
whether the position is different on the variant protein).
TABLE-US-00576 TABLE 11 Glycosylation site(s) Position(s) on known
Present in amino acid sequence variant protein? 1640 no
[1729] Variant protein HUMCA1XIA_P15 (SEQ ID NO:351) is encoded by
the following transcript(s): HUMCA1XIA_T17 (SEQ ID NO:299), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HUMCA1XIA_T17 (SEQ ID NO:299) is
shown in bold; this coding portion starts at position 319 and ends
at position 2505. The transcript also has the following SNPs as
listed in Table 12 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P15 (SEQ ID NO:351)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00577 TABLE
12 Nucleic acid SNPs SNP position on Alternative Previously
nucleotide sequence nucleic acid known SNP? 157 A->G No 241
T->A Yes 340 T->G Yes 456 T->G Yes 1993 G->A Yes 2473
C->T Yes
[1730] Variant protein HUMCA1XIA_P16 (SEQ ID NO:352) according to
the present invention has an amino acid sequence as given at the
end of the application; it is encoded by transcript(s)
HUMCA1XIA_T19 (SEQ ID NO:300). An alignment is given to the known
protein (Collagen alpha 1 (SEQ ID NO:348)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1731] Comparison report between HUMCA1XIA_P16 (SEQ ID NO:352) and
CA1B_HUMAN (SEQ ID NO:348):
[1732] 1. An isolated chimeric polypeptide encoding for
HUMCA1XIA_P16 (SEQ ID NO:352), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQT
EANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDSQRKNSED
TLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAETDITETSIN
GHGAYGEKGQKGEPAWEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPG
RPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPM
GLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMP
GEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEA
corresponding to amino acids 1-648 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 1-648 of HUMCA1XIA_P16 (SEQ
ID NO:352), a second amino acid sequence being at least 90%
homologous to GMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEK
corresponding to amino acids 667-714 of CA1B_HUMAN (SEQ ID NO:348),
which also corresponds to amino acids 649-696 of HUMCA1XIA_P16 (SEQ
ID NO:352), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
VSFSFSLFYKKVIKFACDKRFVGRHDERKWKLSLPLYLIYE (SEQ ID NO:974)
corresponding to amino acids 697-738 of HUMCA1XIA_P16 (SEQ ID
NO:352), wherein said first amino acid sequence, second amino acid
sequence and third amino acid sequence are contiguous and in a
sequential order.
[1733] 2. An isolated chimeric polypeptide encoding for an edge
portion of HUMCA1XIA_P16 (SEQ ID NO:352), comprising a polypeptide
having a length "n", wherein n is at least about 10 amino acids in
length, optionally at least about 20 amino acids in length,
preferably at least about 30 amino acids in length, more preferably
at least about 40 amino acids in length and most preferably at
least about 50 amino acids in length, wherein at least two amino
acids comprise AG, having a structure as follows: a sequence
starting from any of amino acid numbers 648-x to 648; and ending at
any of amino acid numbers 649+((n-2)-x), in which x varies from 0
to n-2.
[1734] 3. An isolated polypeptide encoding for a tail of
HUMCA1XIA_P16 (SEQ ID NO:352), comprising a polypeptide being at
least 70%, optionally at least about 80%, preferably at least about
85%, more preferably at least about 90% and most preferably at
least about 95% homologous to the sequence
VSFSFSLFYKKVIKFACDKRFVGRHDERKVVKLSLPLYLIYE (SEQ ID NO:974) in
HUMCA1XIA_P16 (SEQ ID NO:352).
[1735] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1736] Variant protein HUMCA1XIA_P16 (SEQ ID NO:352) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P16 (SEQ ID NO:352)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00578 TABLE
13 Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 8 W->G Yes 46
D->E Yes 559 G->S Yes
[1737] The glycosylation sites of variant protein HUMCA1XIA_P16
(SEQ ID NO:352), as compared to the known protein Collagen alpha 1
(SEQ ID NO:348), are described in Table 14 (given according to
their position(s) on the amino acid sequence in the first column;
the second column indicates whether the glycosylation site is
present in the variant protein; and the last column indicates
whether the position is different on the variant protein).
TABLE-US-00579 TABLE 14 Glycosylation site(s) Position(s) on known
Present in amino acid sequence variant protein? 1640 no
[1738] Variant protein HUMCA1XIA_P16 (SEQ ID NO:352) is encoded by
the following transcript(s): HUMCA1XIA_T19 (SEQ ID NO:300), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HUMCA1XIA_T19 (SEQ ID NO:300) is
shown in bold; this coding portion starts at position 319 and ends
at position 2532. The transcript also has the following SNPs as
listed in Table 15 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P16 (SEQ ID NO:352)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00580 TABLE
15 Nucleic acid SNPs SNP position on Alternative Previously
nucleotide sequence nucleic acid known SNP? 157 A->G No 241
T->A Yes 340 T->G Yes 456 T->G Yes 1993 G->A Yes 2606
C->A Yes 2677 T->G Yes 2849 C->T Yes
[1739] Variant protein HUMCA1XIA_P17 (SEQ ID NO:353) according to
the present invention has an amino acid sequence as given at the
end of the application; it is encoded by transcript(s)
HUMCA1XIA_T20 (SEQ ID NO:301). An alignment is given to the known
protein (Collagen alpha 1 (SEQ ID NO:348)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[1740] Comparison report between HUMCA1XIA_P17 (SEQ ID NO:353) and
CA1B_HUMAN (SEQ ID NO:348):
[1741] 1. An isolated chimeric polypeptide encoding for
HUMCA1XIA_P17 (SEQ ID NO:353), comprising a first amino acid
sequence being at least 90% homologous to
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGMPVDVLKALDFHNSPEGISKTT
GFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIY
NEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTM
IVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEH
YSPDCDSSAPKAAQAQEPQIDE corresponding to amino acids 1-260 of
CA1B_HUMAN (SEQ ID NO:348), which also corresponds to amino acids
1-260 of HUMCA1XIA_P17 (SEQ ID NO:353), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VRSTRPEKVFVFQ (SEQ ID NO:975) corresponding to amino acids 261-273
of HUMCA1XIA_P17 (SEQ ID NO:353), wherein said first amino acid
sequence and second amino acid sequence are contiguous and in a
sequential order.
[1742] 2. An isolated polypeptide encoding for a tail of HUMCA1
XIA_P17 (SEQ ID NO:353), comprising a polypeptide being at least
70%, optionally at least about 80%, preferably at least about 85%,
more preferably at least about 90% and most preferably at least
about 95% homologous to the sequence VRSTRPEKVFVFQ (SEQ ID NO:975)
in HUMCA1XIA_P17 (SEQ ID NO:353).
[1743] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1744] Variant protein HUMCA1XIA_P17 (SEQ ID NO:353) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 16, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P17 (SEQ ID NO:353)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00581 TABLE
16 Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 8 W->G Yes 46
D->E Yes
[1745] The glycosylation sites of variant protein HUMCA1XIA_P17
(SEQ ID NO:353), as compared to the known protein Collagen alpha 1
(SEQ ID NO:348), are described in Table 17 (given according to
their position(s) on the amino acid sequence in the first column;
the second column indicates whether the glycosylation site is
present in the variant protein; and the last column indicates
whether the position is different on the variant protein).
TABLE-US-00582 TABLE 17 Glycosylation site(s) Position(s) on known
Present in Position in amino acid sequence variant protein? variant
protein? 1640 no
[1746] Variant protein HUMCA1XIA_P17 (SEQ ID NO:353) is encoded by
the following transcript(s): HUMCA XIA_T20 (SEQ ID NO:301), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HUMCA1XIA_T20 (SEQ ID NO:301) is
shown in bold; this coding portion starts at position 319 and ends
at position 1137. The transcript also has the following SNPs as
listed in Table 18 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HUMCA1XIA_P17 (SEQ ID NO:353)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00583 TABLE
18 Nucleic acid SNPs SNP position on Alternative Previously
nucleotide sequence nucleic acid known SNP? 157 A->G No 241
T->A Yes 340 T->G Yes 456 T->G Yes 1150 A->C Yes
[1747] As noted above, cluster HUMCA1XIA features 46 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1748] Segment cluster HUMCA1XIA_node.sub.--0 (SEQ ID NO:302)
according to the present invention is supported by 13 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMCA1XIA_T16 (SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299),
HUMCA1XIA_T19 (SEQ ID NO:300) and HUMCA1XIA_T20 (SEQ ID NO:301).
Table 19 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00584 TABLE 19 Segment
location on transcripts Segment Segment Transcript name starting
position ending position HUMCA1XIA_T16 (SEQ ID NO:298) 1 424
HUMCA1XIA_T17 (SEQ ID NO:299) 1 424 HUMCA1XIA_T19 (SEQ ID NO:300) 1
424 HUMCA1XIA_T20 (SEQ ID NO:301) 1 424
[1749] Segment cluster HUMCA1XIA_node.sub.--2 (SEQ ID NO:303)
according to the present invention is supported by 9 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299), HUMCA1XIA_T19 (SEQ
ID NO:300) and HUMCA1XIA_T20 (SEQ ID NO:301). Table 20 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00585 TABLE 20 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T16 (SEQ ID NO:298) 425 592 HUMCA1XIA T17 (SEQ ID NO:299)
425 592 HUMCA1XIA_T19 (SEQ ID NO:300) 425 592 HUMCA1XIA_T20 (SEQ ID
NO:301) 425 592
[1750] Segment cluster HUMCA1XIA_node.sub.--4 (SEQ ID NO:304)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299), HUMCA1XIA_T19 (SEQ
ID NO:300) and HUMCA1XIA_T20 (SEQ ID NO:301). Table 21 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00586 TABLE 21 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T16 (SEQ ID NO:298) 593 806 HUMCA1XIA_T17 (SEQ ID NO:299)
593 806 HUMCA1XIA_T19 (SEQ ID NO:300) 593 806 HUMCA1XIA_T20 (SEQ ID
NO:301) 593 806
[1751] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 22. TABLE-US-00587 TABLE
22 Oligonucleotides related to this segment Overexpressed Chip
Oligonucleotide name in cancers reference
HUMCA1XIA.sub.--0.sub.--18.sub.--0 breast malignant BRS (SEQ ID
NO:904) tumors
[1752] Segment cluster HUMCA1XIA_node.sub.--6 (SEQ ID NO:305)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299), HUMCA1XIA_T19 (SEQ
ID NO:300) and HUMCA1XIA_T20 (SEQ ID NO:301). Table 23 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00588 TABLE 23 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T16 (SEQ ID NO:298) 807 969 HUMCA1XIA_T17 (SEQ ID NO:299)
807 969 HUMCA1XIA_T19 (SEQ ID NO:300) 807 969 HUMCA1XIA_T20 (SEQ ID
NO:301) 807 969
[1753] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 24. TABLE-US-00589 TABLE
24 Oligonucleotides related to this segment Overexpressed Chip
Oligonucleotide name in cancers reference
HUMCA1XIA.sub.--0.sub.--18.sub.--0 breast malignant BRS (SEQ ID
NO:904) tumors
[1754] Segment cluster HUMCA1XIA_node.sub.--8 (SEQ ID NO:306)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299), HUMCA1XIA_T19 (SEQ
ID NO:300) and HUMCA1XIA_T20 (SEQ ID NO:301). Table 25 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00590 TABLE 25 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T16 (SEQ ID NO:298) 970 1098 HUMCA1XIA_T17 (SEQ ID
NO:299) 970 1098 HUMCA1XIA_T19 (SEQ ID NO:300) 970 1098
HUMCA1XIA_T20 (SEQ ID NO:301) 970 1098
[1755] Segment cluster HUMCA1XIA_node.sub.--9 (SEQ ID NO:307)
according to the present invention is supported by 2 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T20
(SEQ ID NO:301). Table 26 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00591 TABLE
26 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T20 (SEQ ID NO:301)
1099 1271
[1756] Segment cluster HUMCA1XIA_node.sub.--18 (SEQ ID NO:308)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 27 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00592 TABLE
27 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1309 1522 HUMCA1XIA_T17 (SEQ ID NO:299) 1309 1522 HUMCA1XIA_T19
(SEQ ID NO:300) 1309 1522
[1757] Segment cluster HUMCA1XIA_node.sub.--54 (SEQ ID NO:309)
according to the present invention is supported by 2 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T19
(SEQ ID NO:300). Table 28 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00593 TABLE
28 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T19 (SEQ ID NO:300)
2407 2836
[1758] Segment cluster HUMCA1XIA_node.sub.--55 (SEQ ID NO:310)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T17
(SEQ ID NO:299) and HUMCA1XIA_T19 (SEQ ID NO:300). Table 29 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00594 TABLE 29 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T17 (SEQ ID NO 299) 2461 2648 HUMCA1XIA_T19 (SEQ ID NO
300) 2837 3475
[1759] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 30. TABLE-US-00595 TABLE
30 Oligonucleotides related to this segment Overexpressed Chip
Oligonucleotide name in cancers reference
HUMCA1XIA.sub.--0.sub.--0.sub.--14909 breast malignant BRS (SEQ ID
NO:903) tumors
[1760] Segment cluster HUMCA1XIA_node.sub.--92 (SEQ ID NO:311)
according to the present invention is supported by 2 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00596 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
3487 3615
[1761] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1762] Segment cluster HUMCA1XIA_node.sub.--11 (SEQ ID NO:312)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 32 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00597 TABLE
32 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1099 1215 HUMCA1XIA_T17 (SEQ ID NO:299) 1099 1215 HUMCA1XIA_T19
(SEQ ID NO:300) 1099 1215
[1763] Segment cluster HUMCA1XIA_node.sub.--15 (SEQ ID NO:313)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 33 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00598 TABLE
33 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1216 1308 HUMCA1XIA_T17 (SEQ ID NO:299) 1216 1308 HUMCA1XIA_T19
(SEQ ID NO:300) 1216 1308
[1764] Segment cluster HUMCA1XIA_node.sub.--19 (SEQ ID NO:314)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 34 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00599 TABLE
34 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1523 1563 HUMCA1XIA_T17 (SEQ ID NO:299) 1523 1563 HUMCA1XIA_T19
(SEQ ID NO:300) 1523 1563
[1765] Segment cluster HUMCA1XIA_node.sub.--21 (SEQ ID NO:315)
according to the present invention is supported by 2 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 35 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00600 TABLE
35 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1564 1626 HUMCA1XIA_T17 (SEQ ID NO:299) 1564 1626 HUMCA1XIA_T19
(SEQ ID NO:300) 1564 1626
[1766] Segment cluster HUMCA1XIA_node.sub.--23 (SEQ ID NO:316)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 36 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00601 TABLE
36 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1627 1668 HUMCA1XIA_T17 (SEQ ID NO:299) 1627 1668 HUMCA1XIA_T19
(SEQ ID NO:300) 1627 1668
[1767] Segment cluster HUMCA1XIA_node.sub.--25 (SEQ ID NO:317)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 37 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00602 TABLE
37 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1669 1731 HUMCA1XIA_T17 (SEQ ID NO:299) 1669 1731 HUMCA1XIA_T19
(SEQ ID NO:300) 1669 1731
[1768] Segment cluster HUMCA1XIA_node.sub.--27 (SEQ ID NO:318)
according to the present invention is supported by 2 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 38 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00603 TABLE
38 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA T16 (SEQ ID NO:298)
1732 1806 HUMCA1XIA_T17 (SEQ ID NO:299) 1732 1806 HUMCA1XIA_T19
(SEQ ID NO:300) 1732 1806
[1769] Segment cluster HUMCA1XIA_node.sub.--29 (SEQ ID NO:319)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 39 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00604 TABLE
39 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1807 1890 HUMCA1XIA_T17 (SEQ ID NO:299) 1807 1890 HUMCA1XIA_T19
(SEQ ID NO:300) 1807 1890
[1770] Segment cluster HUMCA1XIA_node.sub.--31 (SEQ ID NO:320)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO: 298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 40 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00605 TABLE
40 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1891 1947 HUMCA1XIA_T17 (SEQ ID NO:299) 1891 1947 HUMCA1XIA_T19
(SEQ ID NO:300) 1891 1947
[1771] Segment cluster HUMCA1XIA_node.sub.--33 (SEQ ID NO:321)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 41 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00606 TABLE
41 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
1948 2001 HUMCA1XIA_T17 (SEQ ID NO:299) 1948 2001 HUMCA1XIA_T19
(SEQ ID NO:300) 1948 2001
[1772] Segment cluster HUMCA1XIA_node.sub.--35 (SEQ ID NO:322)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 42 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00607 TABLE
42 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2002 2055 HUMCA1XIA_T17 (SEQ ID NO:299) 2002 2055 HUMCA1XIA_T19
(SEQ ID NO:300) 2002 2055
[1773] Segment cluster HUMCA1XIA_node.sub.--37 (SEQ ID NO:323)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 43 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00608 TABLE
43 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2056 2109 HUMCA1XIA_T17 (SEQ ID NO:299) 2056 2109 HUMCA1XIA_T19
(SEQ ID NO:300) 2056 2109
[1774] Segment cluster HUMCA1XIA_node.sub.--39 (SEQ ID NO:324)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 44 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00609 TABLE
44 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2110 2163 HUMCA1XIA_T17 (SEQ ID NO:299) 2110 2163 HUMCA1XIA_T19
(SEQ ID NO:300) 2110 2163
[1775] Segment cluster HUMCA1XIA_node.sub.--41 (SEQ ID NO:325)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 45 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00610 TABLE
45 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2164 2217 HUMCA1XIA_T17 (SEQ ID NO:299) 2164 2217 HUMCA1XIA_T19
(SEQ ID NO:300) 2164 2217
[1776] Segment cluster HUMCA1XIA_node.sub.--43 (SEQ ID
NO:326),according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMCA1XIA_T16 (SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID
NO:299) and HUMCA1XIA_T19 (SEQ ID NO:300). Table 46 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00611 TABLE 46 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCAIXIA_T16 (SEQ ID NO:298) 2218 2262 HUMCA1XIA_T17 (SEQ ID
NO:299) 2218 2262 HUMCA1XIA_T19 (SEQ ID NO:300) 2218 2262
[1777] Segment cluster HUMCA1XIA_node.sub.--45 (SEQ ID NO:327)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298) and HUMCA1XIA_T17 (SEQ ID NO:299). Table 47 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00612 TABLE 47 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HUMCA1XIA_T16 (SEQ ID NO:298) 2263 2316 HUMCA1XIA_T17 (SEQ ID
NO:299) 2263 2316
[1778] Segment cluster HUMCA1XIA_node.sub.--47 (SEQ ID NO:328)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298, HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 48 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00613 TABLE
48 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2317 2361 HUMCA1XIA_T17 (SEQ ID NO:299) 2317 2361 HUMCA1XIA_T19
(SEQ ID NO:300) 2263 2307
[1779] Segment cluster HUMCA1XIA_node.sub.--49 (SEQ ID NO:329)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 49 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00614 TABLE
49 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2362 2415 HUMCA1XIA_T17 (SEQ ID NO:299) 2362 2415 HUMCA1XIA_T19
(SEQ ID NO:300) 2308 2361
[1780] Segment cluster HUMCA1XIA_node.sub.--51 (SEQ ID NO:330)
according to the present invention is supported by 7 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298), HUMCA1XIA_T17 (SEQ ID NO:299) and HUMCA1XIA_T19
(SEQ ID NO:300). Table 50 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00615 TABLE
50 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2416 2460 HUMCA1XIA_T17 (SEQ ID NO:299) 2416 2460 HUMCA1XIA_T19
(SEQ ID NO:300) 2362 2406
[1781] Segment cluster HUMCA1XIA_node.sub.--57 (SEQ ID NO:331)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 51 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00616 TABLE
51 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2461 2514
[1782] Segment cluster HUMCA1XIA_node.sub.--59 (SEQ ID NO:332)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 52 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00617 TABLE
52 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2515 2559
[1783] Segment cluster HUMCA1XIA_node.sub.--62 (SEQ ID NO:333)
according to the present invention is supported by 3 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 53 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00618 TABLE
53 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2560 2613
[1784] Segment cluster HUMCA1XIA_node.sub.--64 (SEQ ID NO:334)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 54 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00619 TABLE
54 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2614 2658
[1785] Segment cluster HUMCA1XIA_node.sub.--66 (SEQ ID NO:335)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 55 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00620 TABLE
55 Segment location on transcripts Segment Segment Transcript name
starting position ending position HUMCA1XIA_T16 (SEQ ID NO:298)
2659 2712
[1786] Segment cluster HUMCA1XIA_node.sub.--68 (SEQ ID NO:336)
according to the present invention is supported by 7 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 56 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00621 TABLE
56 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
2713 2820
[1787] Segment cluster HUMCA1XIA_node.sub.--70 (SEQ ID NO:337)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 57 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00622 TABLE
57 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
2821 2874
[1788] Segment cluster HUMCA1XIA_node.sub.--72 (SEQ ID NO:338)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 58 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00623 TABLE
58 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
2875 2928
[1789] Segment cluster HUMCA1XIA_node.sub.--74 (SEQ ID NO:339)
according to the present invention is supported by 5 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 59 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00624 TABLE
59 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
2929 2973
[1790] Segment cluster HUMCA1XIA_node.sub.--76 (SEQ ID NO:340)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 60 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00625 TABLE
60 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
2974 3027
[1791] Segment cluster HUMCA1XIA_node.sub.--78 (SEQ ID NO:341)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 61 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00626 TABLE
61 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
3028 3072
[1792] Segment cluster HUMCA1XIA_node.sub.--81 (SEQ ID NO:342)
according to the present invention is supported by 8 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 62 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00627 TABLE
62 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
3073 3126
[1793] Segment cluster HUMCA1XIA_node.sub.--83 (SEQ ID NO:343)
according to the present invention is supported by 7 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 63 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00628 TABLE
63 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
3127 3180
[1794] Segment cluster HUMCA1XIA_node.sub.--85 (SEQ ID NO:344)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 64 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00629 TABLE
64 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
3181 3234
[1795] Segment cluster HUMCA1XIA_node.sub.--87 (SEQ ID NO:345)
according to the present invention is supported by 10 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMCA1XIA_T16 (SEQ ID NO:298). Table 65 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00630 TABLE 65 Segment location on transcripts Segment
Segment starting ending Transcript name position position
HUMCA1XIA_T16 (SEQ ID NO:298) 3235 3342
[1796] Segment cluster HUMCA1XIA_node.sub.--89 (SEQ ID NO:346)
according to the present invention is supported by 9 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): HUMCA1XIA_T16
(SEQ ID NO:298). Table 66 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00631 TABLE
66 Segment location on transcripts Segment Segment starting ending
Transcript name position position HUMCA1XIA_T16 (SEQ ID NO:298)
3343 3432
[1797] Segment cluster HUMCA1XIA_node.sub.--91 (SEQ ID NO:347)
according to the present invention is supported by 11 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMCA1XIA_T16 (SEQ ID NO:298). Table 67 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00632 TABLE 67 Segment location on transcripts Segment
Segment starting ending Transcript name position position
HUMCA1XIA_T16 (SEQ ID NO:298) 3433 3486
[1798] Transcript nucleic acid sequences:
[1799] Variant protein alignment to the previously known
protein:
[1800] Sequence name: CA1B_HUMAN_V5 (SEQ ID NO:349)
[1801] Sequence Documentation:
[1802] Alignment of: HUMCA1XIA_P14 (SEQ ID
NO:350).times.CA1B_HUMAN_V5 (SEQ ID NO:349).
[1803] Alignment segment 1/1: TABLE-US-00633 Quality: 10456.00
Escore: 0 Matching length: 1058 Total length: 1058 Matching Percent
99.91 Matching Percent 99.91 Similarity: Identity: Total Percent
Similarity: 99.91 Total Percent Identity: 99.91 Gaps: 0
TABLE-US-00058 Alignment: TABLE-US-00634 . . . 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50 . . . 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100 . . . 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150 . . . 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200 . . . 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250 . . . 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300 . . . 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350 . . . 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400 . . . 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450 . . . 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500 . . . 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550 . . . 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600 . . . 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGP 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGP 650 . . . 651
RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 700 . . . 701
LPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPP 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
LPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPP 750 . . . 751
GPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGE 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
GPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGE 800 . . . 801
VGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQG 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
VGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQG 850 . . . 851
PKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARGPTGKP 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
PKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARGPTGKP 900 . . . 901
GPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQ 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
GPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQ 950 . . . 951
RGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPG 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
RGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPG 1000 . . . 1001
AAGKEGAKGDPGPQGISGKDGPAGLRGFPGERGLPGAQGAPGLKGGEGPQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
AAGKEGAKGDPGPQGISGKDGPAGLRGFPGERGLPGAQGAPGLKGGEGPQ 1050 1051
GPPGPVVS 1058 |||||| | 1051 GPPGPVGS 1058
[1804] Sequence name: CA1B_HUMAN (SEQ ID NO:348)
[1805] Sequence Documentation:
[1806] Alignment of: HUMCA1XIA_P15 (SEQ ID NO:351).times.CA1B_HUMAN
(SEQ ID NO:348).
[1807] Alignment segment 1/1: TABLE-US-00635 Quality: 7073.00
Escore: 0 Matching length: 714 Total length: 714 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00059 Alignment: TABLE-US-00636 . . . 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50 . . . 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100 . . . 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150 . . . 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200 . . . 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250 . . . 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300 . . . 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350 . . . 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400 . . . 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450 . . . 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500 . . . 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550 . . . 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600 . . . 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGP 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGP 650 . . . 651
RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 700 . . . 701
LPGPQGPIGPPGEK 714 |||||||||||||| 701 LPGPQGPIGPPGEK 714
[1808] Sequence name: CA1B_HUMAN (SEQ ID NO:348)
[1809] Sequence Documentation:
[1810] Alignment of: HUMCA1XIA_P16 (SEQ ID NO:352).times.CA1B_HUMAN
(SEQ ID NO:348).
[1811] Alignment segment 1/1: TABLE-US-00637 Quality: 6795.00
Escore: 0 Matching length: 696 Total length: 714 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 97.48 Total Percent Identity: 97.48 Gaps: 1
TABLE-US-00060 Alignment: TABLE-US-00638 . . . 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50 . . . 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100 . . . 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150 . . . 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200 . . . 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250 . . . 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
AQAQEPQIDEYAPEDIIEYDYEYGEAEYKEAESVTEGPTVTEETIAQTEA 300 . . . 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
NIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDYDS 350 . . . 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
QRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEE 400 . . . 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
FGPGVPAETDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPA 450 . . . 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GIMGPPGLQGPTGPPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYG 500 . . . 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
GDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSSG 550 . . . 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
AKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDR 600 . . . 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEA..648
|||||||||||||||||||||||||||||||||||||||||||||||| 601
GFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGP 650 . . . 649
................GMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 682
|||||||||||||||||||||||||||||||||| 651
RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQG 700 . . . 683
LPGPQGPIGPPGEK 696 |||||||||||||| 701 LPGPQGPIGPPGEK 714
[1812] Sequence name: CA1B_HUMAN (SEQ ID NO:348)
[1813] Sequence Documentation:
[1814] Alignment of: HUMCA1XIA_P17 (SEQ ID NO:353).times.CA1B HUMAN
(SEQ ID NO:348).
[1815] Alignment segment 1/1: TABLE-US-00639 Quality: 2561.00
Escore: 0 Matching length: 260 Total length: 260 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00061 Alignment: TABLE-US-00640 . . . 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNS 50 . . . 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFS 100 . . . 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPED 150 . . . 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
YPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDT 200 . . . 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
NGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKA 250 . . . 251
AQAQEPQIDE 260 |||||||||| 251 AQAQEPQIDE 260
Description for Cluster R20779
[1816] Cluster R20779 features 1 transcript(s) and 24 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00641 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. R20779_T7 354
[1817] TABLE-US-00642 TABLE 2 Segments of interest Segment Name
Sequence ID No. R20779_node.sub.--0 355 R20779_node.sub.--2 356
R20779_node.sub.--7 357 R20779_node.sub.--9 358
R20779_node.sub.--18 359 R20779_node.sub.--21 360
R20779_node.sub.--24 361 R20779_node.sub.--27 362
R20779_node.sub.--28 363 R20779_node.sub.--30 364
R20779_node.sub.--31 365 R20779_node.sub.--32 366
R20779_node.sub.--1 367 R20779_node.sub.--3 368
R20779_node.sub.--10 369 R20779_node.sub.--11 370
R20779_node.sub.--14 371 R20779_node.sub.--17 372
R20779_node.sub.--19 373 R20779_node.sub.--20 374
R20779_node.sub.--22 375 R20779_node.sub.--23 376
R20779_node.sub.--25 377 R20779_node.sub.--29 378
[1818] TABLE-US-00643 TABLE 3 Proteins of interest Protein Name
Sequence ID No. Corresponding Transcript(s) R20779_P2 380 R20779_T7
(SEQ ID NO: 354)
[1819] These sequences are variants of the known protein
Stanniocalcin 2 precursor (SEQ ID NO:379) (SwissProt accession
identifier STC2_HUMAN; known also according to the synonyms STC-2;
Stanniocalcin-related protein; STCRP; STC-related protein), SEQ ID
NO: 379, referred to herein as the previously known protein.
[1820] Protein Stanniocalcin 2 precursor (SEQ ID NO:379) is known
or believed to have the following function(s): Has an
anti-hypocalcemic action on calcium and phosphate homeostasis. The
sequence for protein Stanniocalcin 2 precursor (SEQ ID NO:379) is
given at the end of the application, as "Stanniocalcin 2 precursor
(SEQ ID NO:379) amino acid sequence". Protein Stanniocalcin 2
precursor (SEQ ID NO:379) localization is believed to be Secreted
(Potential).
[1821] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: cell surface
receptor linked signal transduction; cell-cell signaling;
nutritional response pathway, which are annotation(s) related to
Biological Process; hormone, which are annotation(s) related to
Molecular Function; and extracellular, which are annotation(s)
related to Cellular Component.
[1822] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1823] Cluster R20779 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 33 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1824] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 33 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues and lung malignant tumors.
TABLE-US-00644 TABLE 4 Normal tissue distribution Name of Tissue
Number Bone 825 Brain 0 Colon 0 epithelial 32 general 38 kidney 22
Liver 9 Lung 11 Lymph nodes 0 Breast 215 muscle 35 Ovary 36
pancreas 4 prostate 80 Skin 99 stomach 0 Uterus 4
[1825] TABLE-US-00645 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 Bone 5.9e-01
7.4e-01 1 0.2 1 0.1 Brain 2.5e-02 1.6e-02 2.2e-01 6.0 3.5e-02 8.0
Colon 1.7e-01 1.7e-01 1 1.3 7.7e-01 1.5 epithelial 1.7e-01 1.5e-03
5.9e-01 1.0 2.0e-04 2.0 general 2.4e-02 6.2e-07 7.6e-01 0.8 4.6e-05
1.6 kidney 4.3e-01 2.7e-01 6.2e-01 1.3 1.5e-01 2.0 Liver 8.3e-01
7.6e-01 1 0.8 3.3e-01 1.6 Lung 1.2e-01 1.4e-03 1.9e-01 2.9 1.6e-05
7.7 Lymph nodes 1 3.1e-01 1 1.0 1 1.4 Breast 6.8e-01 6.8e-01
6.9e-01 0.8 3.6e-01 0.8 muscle 9.2e-01 4.8e-01 1 0.3 1.4e-03 1.4
Ovary 8.4e-01 7.1e-01 9.0e-01 0.7 8.6e-01 0.8 pancreas 9.3e-01
6.8e-01 1 0.7 1.5e-01 2.0 prostate 9.1e-01 5.0e-01 9.8e-01 0.4
5.7e-01 0.7 Skin 6.3e-01 7.5e-01 7.1e-01 0.8 9.5e-01 0.3 stomach 1
4.5e-01 1 1.0 5.1e-01 1.8 Uterus 7.1e-01 2.6e-01 4.4e-01 1.7 4.1
e-01 1.8
[1826] For this cluster, at least one oligonucleotide was found to
demonstrate overexpression of the cluster, although not of at least
one transcript/segment as listed below. Microarray (chip) data is
also available for this cluster as follows. Various
oligonucleotides were tested for being differentially expressed in
various disease conditions, particularly cancer, as previously
described. The following oligonucleotides were found to hit this
cluster but not other segments/transcripts below, shown in Table 6.
TABLE-US-00646 TABLE 6 Oligonucleotides related to this cluster
Oligonucleotide name Overexpressed in cancers Chip reference
R20779.sub.--0.sub.--0.sub.--30670 (SEQ breast malignant tumors BRS
ID NO: 905)
[1827] As noted above, cluster R20779 features 1 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Stanniocalcin 2
precursor (SEQ ID NO:379). A description of each variant protein
according to the present invention is now provided.
[1828] Variant protein R20779_P2 (SEQ ID NO:380) according to the
present invention has an amino acid sequence as given at the end of
the application; it is encoded by transcript(s) R20779_T7 (SEQ ID
NO:354). An alignment is given to the known protein (Stanniocalcin
2 precursor (SEQ ID NO:379)) at the end of the application. One or
more alignments to one or more previously published protein
sequences are given at the end of the application. A brief
description of the relationship of the variant protein according to
the present invention to each such aligned protein is as
follows:
[1829] Comparison report between R20779_P2 (SEQ ID NO:380) and
STC2_HUMAN (SEQ ID NO:379):
[1830] 1. An isolated chimeric polypeptide encoding for R20779_P2
(SEQ ID NO:380), comprising a first amino acid sequence being at
least 90% homologous to
MCAERLGQFMTLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLV
NAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRH
RFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHE corresponding
to amino acids 1-169 of STC2_HUMAN (SEQ ID NO:379), which also
corresponds to amino acids 1-169 of R20779_P2 (SEQ ID NO:380), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence CYKIEITMPKRRKVKLRD (SEQ ID NO:976) corresponding to
amino acids 170-187 of R20779_P2 (SEQ ID NO:380), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[1831] 2. An isolated polypeptide encoding for a tail of R20779_P2
(SEQ ID NO:380), comprising a polypeptide being at least 70%,
optionally at least about 80%, preferably at least about 85%, more
preferably at least about 90% and most preferably at least about
95% homologous to the sequence CYKIEITMPKRRKVKLRD (SEQ ID NO:976)
in R20779_P2 (SEQ ID NO:380).
[1832] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1833] Variant protein R20779_P2 (SEQ ID NO:380) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R20779_P2 (SEQ ID NO:380) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00647 TABLE 7 Amino
acid mutations SNP position(s) on Alternative Previously amino acid
sequence amino acid(s) known SNP? 16 L->No 98 Q->No 171
Y->C Yes 177 M->V Yes
[1834] The glycosylation sites of variant protein R20779_P2 (SEQ ID
NO:380), as compared to the known protein Stanniocalcin 2 precursor
(SEQ ID NO:379), are described in Table 8 (given according to their
position(s) on the amino acid sequence in the first column; the
second column indicates whether the glycosylation site is present
in the variant protein; and the last column indicates whether the
position is different on the variant protein). TABLE-US-00648 TABLE
8 Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 73 yes 73
[1835] Variant protein R20779_P2 (SEQ ID NO:380) is encoded by the
following transcript(s): R20779_T7 (SEQ ID NO:354), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript R20779_T7 (SEQ ID NO:354) is shown in bold;
this coding portion starts at position 1397 and ends at position
1957. The transcript also has the following SNPs as listed in Table
9 (given according to their position on the nucleotide sequence,
with the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein R20779_P2 (SEQ ID NO:380) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00649 TABLE 9 Nucleic acid SNPs SNP
position on Alternative Previously nucleotide sequence nucleic acid
known SNP? 1442 T->No 1690 G->No 1732 C->T Yes 1867
G->T Yes 1908 A->G Yes 1925 A->G Yes 1968 G->A Yes 2087
C->T No 2138 C->T Yes 2270 C->No 2443 A->No 2478
G->No 2479 C->A No 2616 C->A No 2941 C->No 3196->A
No 3479 T->G Yes 4290 C->T Yes 4358 G->A Yes 5363 G->A
No
[1836] As noted above, cluster R20779 features 24 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[1837] Segment cluster R20779_node.sub.--0 (SEQ ID NO:355)
according to the present invention is supported by 31 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 10 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00650 TABLE
10 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1
1298
[1838] Segment cluster R20779_node.sub.--2 (SEQ ID NO:356)
according to the present invention is supported by 55 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 11 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00651 TABLE
11 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1337
1506
[1839] Segment cluster R20779_node.sub.--7 (SEQ ID NO:357)
according to the present invention is supported by 63 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 12 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00652 TABLE
12 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1548
1690
[1840] Segment cluster R20779_node.sub.--9 (SEQ ID NO:358)
according to the present invention is supported by 66 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 13 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00653 TABLE
13 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1691
1838
[1841] Segment cluster R20779_node.sub.--18 (SEQ ID NO:359)
according to the present invention is supported by 61 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 14 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00654 TABLE
14 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2009
2176
[1842] Segment cluster R20779_node.sub.--21 (SEQ ID NO:360)
according to the present invention is supported by 106 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 15 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00655 TABLE
15 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2219
2796
[1843] Segment cluster R20779_node.sub.--24 (SEQ ID NO:361)
according to the present invention is supported by 100 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 16 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00656 TABLE
16 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2977
3667
[1844] Segment cluster R20779_node.sub.--27 (SEQ ID NO:362)
according to the present invention is supported by 26 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 17 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00657 TABLE
17 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 3673
3803
[1845] Segment cluster R20779_node.sub.--28 (SEQ ID NO:363)
according to the present invention is supported by 31 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 18 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00658 TABLE
18 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 3804
4050
[1846] Segment cluster R20779_node.sub.--30 (SEQ ID NO:364)
according to the present invention is supported by 34 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 19 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00659 TABLE
19 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 4068
4193
[1847] Segment cluster R20779_node.sub.--31 (SEQ ID NO:365)
according to the present invention is supported by 46 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 20 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00660 TABLE
20 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 4194
4424
[1848] Segment cluster R20779_node.sub.--32 (SEQ ID NO:366)
according to the present invention is supported by 88 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 21 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00661 TABLE
21 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 4425
5503
[1849] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[1850] Segment cluster R20779_node.sub.--1 (SEQ ID NO:367)
according to the present invention is supported by 27 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 22 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00662 TABLE
22 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1299
1336
[1851] Segment cluster R20779_node.sub.--3 (SEQ ID NO:368)
according to the present invention is supported by 52 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 23 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00663 TABLE
23 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1507
1547
[1852] Segment cluster R20779_node.sub.--10 (SEQ ID NO:369)
according to the present invention can be found in the following
transcript(s): R20779_T7 (SEQ ID NO:354). Table 24 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00664 TABLE 24 Segment location on transcripts
Segment Segment Transcript name starting position ending position
R20779_T7 (SEQ ID NO: 354) 1839 1849
[1853] Segment cluster R20779_node.sub.--11 (SEQ ID NO:370)
according to the present invention is supported by 58 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 25 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00665 TABLE
25 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1850
1902
[1854] Segment cluster R20779_node.sub.--14 (SEQ ID NO:371)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s): R20779_T7 (SEQ
ID NO:354). Table 26 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00666 TABLE
26 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1903
1975
[1855] Segment cluster R20779_node.sub.--17 (SEQ ID NO:372)
according to the present invention is supported by 54 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 27 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00667 TABLE
27 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 1976
2008
[1856] Segment cluster R20779_node.sub.--19 (SEQ ID NO:373)
according to the present invention can be found in the following
transcript(s): R20779_T7 (SEQ ID NO:354). Table 28 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00668 TABLE 28 Segment location on transcripts
Segment Segment Transcript name starting position ending position
R20779_T7 (SEQ ID NO: 354) 2177 2188
[1857] Segment cluster R20779_node.sub.--20 (SEQ ID NO:374)
according to the present invention is supported by 53 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 29 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00669 TABLE
29 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2189
2218
[1858] Segment cluster R20779_node.sub.--22 (SEQ ID NO:375)
according to the present invention is supported by 76 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00670 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2797
2899
[1859] Segment cluster R20779_node.sub.--23 (SEQ ID NO:376)
according to the present invention is supported by 81 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s): R20779_T7
(SEQ ID NO:354). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00671 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position R20779_T7 (SEQ ID NO: 354) 2900
2976
[1860] Segment cluster R20779_node.sub.--25 (SEQ ID NO:377)
according to the present invention can be found in the following
transcript(s): R20779_T7 (SEQ ID NO:354). Table 32 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00672 TABLE 32 Segment location on transcripts
Segment Segment Transcript name starting position ending position
R20779_T7 (SEQ ID NO: 354) 3668 3672
[1861] Segment cluster R20779_node.sub.--29 (SEQ ID NO:378)
according to the present invention can be found in the following
transcript(s): R20779_T7 (SEQ ID NO:354). Table 33 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00673 TABLE 33 Segment location on transcripts
Segment Segment Transcript name starting position ending position
R20779_T7 (SEQ ID NO: 354) 4051 4067
[1862] Variant protein alignment to the previously known
protein:
[1863] Sequence name: STC2_HUMAN (SEQ ID NO:379)
[1864] Sequence Documentation:
[1865] Alignment of: R20779_P2 (SEQ ID NO:380).times.STC2.sub.13
HUMAN (SEQ ID NO:379).
[1866] Alignment segment 1/1: TABLE-US-00674 Quality: 1688.00
Escore: 0 Matching length: 171 Total length: 171 Matching Percent
99.42 Matching Percent 99.42 Similarity: Identity: Total Percent
99.42 Total Percent 99.42 Similarity: Identity: Gaps: 0
TABLE-US-00062 Alignment: TABLE-US-00675 . . . 1
MCAERLGQFMTLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNT 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MCAERLGQFMTLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNT 50 . . . 51
AEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGK 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGK 100 . . . 101
SFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQ 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
SFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQ 150 . . . 151
ENTRVIVEMIHFKDLLLHECY 171 ||||||||||||||||||||| 151
ENTRVIVEMIHFKDLLLHEPY 171
Description for Cluster HSS100PCB
[1867] Cluster HSS100PCB features 1 transcript(s) and 3 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00676 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSS100PCB_T1 381
[1868] TABLE-US-00677 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSS100PCB_node.sub.--3 382 HSS100PCB_node.sub.--4
383 HSS100PCB_node.sub.--5 384
[1869] TABLE-US-00678 TABLE 3 Proteins of interest Protein Name
Sequence ID No. Corresponding Transcript(s) HSS100PCB_P3 386
HSS100PCB_T1 (SEQ ID NO: 381)
[1870] These sequences are variants of the known protein S-100P
protein (SEQ ID NO:385) (SwissProt accession identifier
S10P_HUMAN), SEQ ID NO: 385, referred to herein as the previously
known protein.
[1871] The sequence for protein S-100P protein (SEQ ID NO:385) is
given at the end of the application, as "S-100P protein (SEQ ID
NO:385) amino acid sequence". Known polymorphisms for this sequence
are as shown in Table 4. TABLE-US-00679 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 32 E->T 44 F->E
[1872] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: calcium binding;
protein binding, which are annotation(s) related to Molecular
Function.
[1873] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[1874] Cluster HSS100PCB can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 34 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[1875] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 34 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: a mixture of malignant tumors from
different tissues. TABLE-US-00680 TABLE 5 Normal tissue
distribution Name of Tissue Number Bladder 41 Colon 37 Epithelial
38 General 22 Kidney 0 Liver 0 Lung 18 Breast 0 bone marrow 0 Ovary
0 pancreas 0 prostate 46 stomach 553 Uterus 13
[1876] TABLE-US-00681 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 bladder 3.3e-01
2.9e-01 2.9e-02 2.8 3.5e-02 2.8 Colon 3.0e-01 1.9e-01 5.2e-01 1.2
2.4e-01 1.7 epithelial 4.7e-02 1.6e-02 2.0e-01 1.2 6.1e-02 1.3
general 1.1e-03 6.8e-05 1.4e-02 1.5 4.9e-04 1.7 kidney 6.5e-01
7.2e-01 5.8e-01 1.7 7.0e-01 1.4 Liver 9.1e-01 4.9e-01 1 1.0 7.7e-02
2.1 Lung 6.8e-01 7.3e-01 2.2e-02 2.9 1.3e-01 1.7 Breast 2.8e-01
3.2e-01 4.7e-01 2.0 6.8e-01 1.5 bone marrow 1 6.7e-01 1 1.0 2.8e-01
2.8 Ovary 2.6e-01 3.0e-01 4.7e-01 2.0 5.9e-01 1.7 pancreas 3.3e-01
4.4e-01 7.6e-02 3.7 1.5e-01 2.8 prostate 9.1e-01 9.3e-01 5.8e-01
0.6 7.6e-01 0.5 stomach 3.7e-01 3.2e-01 1 0.1 1 0.3 Uterus 9.4e-01
7.0e-01 1 0.6 4.1e-01 1.1
[1877] As noted above, cluster HSS100PCB features 1 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein S-100P protein (SEQ ID
NO:385). A description of each variant protein according to the
present invention is now provided.
[1878] Variant protein HSS100PCB_P3 (SEQ ID NO:386) according to
the present invention has an amino acid sequence as given at the
end of the application; it is encoded by transcript(s) HSS100PCB_T1
(SEQ ID NO:381). The location of the variant protein was determined
according to results from a number of different software programs
and analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1879] Variant protein HSS100PCB_P3 (SEQ ID NO:386) also has the
following non-silent SNPs (Single Nucleotide Polymorphisms) as
listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSS100PCB_P3 (SEQ ID NO:386)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00682 TABLE 7
Amino acid mutations SNP position(s) on Alternative Previously
amino acid sequence amino acid(s) known SNP? 1 M->R Yes 11
M->L Yes 20 L->F Yes
[1880] Variant protein HSS100PCB_P3 (SEQ ID NO:386) is encoded by
the following transcript(s): HSS100PCB_T1 (SEQ ID NO:381), for
which the sequence(s) is/are given at the end of the application.
The coding portion of transcript HSS100PCB_T1 (SEQ ID NO:381) is
shown in bold; this coding portion starts at position 1057 and ends
at position 1533. The transcript also has the following SNPs as
listed in Table 8 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSS100PCB_P3 (SEQ ID NO:386)
sequence provides support for the deduced sequence of this variant
protein according to the present invention). TABLE-US-00683 TABLE 8
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 52 C->T Yes 107 A->C Yes 458
C->T Yes 468 A->G Yes 648 C->T Yes 846 C->G Yes 882
G->A Yes 960 C->T No 965 C->T Yes 1058 T->G Yes
1087A->C Yes 1114 C->T Yes 1968 G->A Yes 1971 C->T Yes
2010 C->A Yes 2099 G->No
[1881] As noted above, cluster HSS100PCB features 3 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[1882] Segment cluster HSS100PCB_node.sub.--3 (SEQ ID NO:382)
according to the present invention is supported by 16 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSS100PCB_T1 (SEQ ID NO:381). Table 9 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00684 TABLE 9 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSS100PCB_T1 (SEQ ID NO: 381) 1 1133
[1883] Segment cluster HSS100PCB_node.sub.--4 (SEQ ID NO:383)
according to the present invention is supported by 29 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSS100PCB_T1 (SEQ ID NO:381). Table 10 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00685 TABLE 10 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSS100PCB_T1 (SEQ ID NO: 381) 1134 1923
[1884] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 11. TABLE-US-00686 TABLE
11 Oligonucleotides related to this segment Oligonucleotide name
Overexpressed in cancers Chip reference
HSS100PCB.sub.--0.sub.--0.sub.--12280 breast malignant tumors BRS
(SEQ ID NO: 906)
[1885] Segment cluster HSS100PCB_node.sub.--5 (SEQ ID NO:384)
according to the present invention is supported by 141 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HSS100PCB_T1 (SEQ ID NO:381). Table 12 below describes the starting
and ending position of this segment on each transcript.
TABLE-US-00687 TABLE 12 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSS100PCB_T1 (SEQ ID NO: 381) 1924 2201
Description for Cluster HSCOC4
[1886] Cluster HSCOC4 features 19 transcript(s) and 79 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00688 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSCOC4_PEA.sub.--1_T1387 HSCOC4_PEA.sub.--1_T2 388
HSCOC4_PEA.sub.--1_T3 389 HSCOC4_PEA.sub.--1_T4 390
HSCOC4_PEA.sub.--1_T5 391 HSCOC4_PEA.sub.--1_T7 392
HSCOC4_PEA.sub.--1_T8 393 HSCOC4_PEA.sub.--1_T11 394
HSCOC4_PEA.sub.--1_T12 395 HSCOC4_PEA.sub.--1_T14 396
HSCOC4_PEA.sub.--1_T15 397 HSCOC4_PEA.sub.--1_T20 398
HSCOC4_PEA.sub.--1_T21 399 HSCOC4_PEA.sub.--1_T25 400
HSCOC4_PEA.sub.--1_T28 401 HSCOC4_PEA.sub.--1_T30 402
HSCOC4_PEA.sub.--1_T31 403 HSCOC4_PEA.sub.--1_T32 404
HSCOC4_PEA.sub.--1_T40 405
[1887] TABLE-US-00689 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSCOC4_PEA.sub.--1_node.sub.--1 406
HSCOC4_PEA.sub.--1_node.sub.--5 407 HSCOC4_PEA.sub.--1_node.sub.--7
408 HSCOC4_PEA.sub.--1_node.sub.--30 409
HSCOC4_PEA.sub.--1_node.sub.--33 410
HSCOC4_PEA.sub.--1_node.sub.--35 411
HSCOC4_PEA.sub.--1_node.sub.--37 412
HSCOC4_PEA.sub.--1_node.sub.--39 413
HSCOC4_PEA.sub.--1_node.sub.--43 414
HSCOC4_PEA.sub.--1_node.sub.--48 415
HSCOC4_PEA.sub.--1_node.sub.--49 416
HSCOC4_PEA.sub.--1_node.sub.--51 417
HSCOC4_PEA.sub.--1_node.sub.--58 418
HSCOC4_PEA.sub.--1_node.sub.--59 419
HSCOC4_PEA.sub.--1_node.sub.--62 420
HSCOC4_PEA.sub.--1_node.sub.--66 421
HSCOC4_PEA.sub.--1_node.sub.--72 422
HSCOC4_PEA.sub.--1_node.sub.--77 423
HSCOC4_PEA.sub.--1_node.sub.--79 424
HSCOC4_PEA.sub.--1_node.sub.--93 425
HSCOC4_PEA.sub.--1_node.sub.--100 426
HSCOC4_PEA.sub.--1_node.sub.--105 427
HSCOC4_PEA.sub.--1_node.sub.--107 428
HSCOC4_PEA.sub.--1_node.sub.--108 429
HSCOC4_PEA.sub.--1_node.sub.--109 430
HSCOC4_PEA.sub.--1_node.sub.--110431
HSCOC4_PEA.sub.--1_node.sub.--112432
HSCOC4_PEA.sub.--1_node.sub.--113 433
HSCOC4_PEA.sub.--1_node.sub.--2 434 HSCOC4_PEA.sub.--1_node.sub.--8
435 HSCOC4_PEA.sub.--1_node.sub.--10 436
HSCOC4_PEA.sub.--1_node.sub.--12 437
HSCOC4_PEA.sub.--1_node.sub.--14 438
HSCOC4_PEA.sub.--1_node.sub.--17 439
HSCOC4_PEA.sub.--1_node.sub.--19 440
HSCOC4_PEA.sub.--1_node.sub.--21 441
HSCOC4_PEA.sub.--1_node.sub.--22 442
HSCOC4_PEA.sub.--1_node.sub.--28 443
HSCOC4_PEA.sub.--1_node.sub.--29 444
HSCOC4_PEA.sub.--1_node.sub.--41 445
HSCOC4_PEA.sub.--1_node.sub.--45 446
HSCOC4_PEA.sub.--1_node.sub.--47 447
HSCOC4_PEA.sub.--1_node.sub.--50 448
HSCOC4_PEA.sub.--1_node.sub.--53 449
HSCOC4_PEA.sub.--1_node.sub.--55 450
HSCOC4_PEA.sub.--1_node.sub.--57 451
HSCOC4_PEA.sub.--1_node.sub.--60 452
HSCOC4_PEA.sub.--1_node.sub.--64 453
HSCOC4_PEA.sub.--1_node.sub.--69 454
HSCOC4_PEA.sub.--1_node.sub.--70 455
HSCOC4_PEA.sub.--1_node.sub.--71 456
HSCOC4_PEA.sub.--1_node.sub.--73 457
HSCOC4_PEA.sub.--1_node.sub.--74 458
HSCOC4_PEA.sub.--1_node.sub.--75 459
HSCOC4_PEA.sub.--1_node.sub.--76 460
HSCOC4_PEA.sub.--1_node.sub.--78 461
HSCOC4_PEA.sub.--1_node.sub.--80 462
HSCOC4_PEA.sub.--1_node.sub.--82 463
HSCOC4_PEA.sub.--1_node.sub.--83 464
HSCOC4_PEA.sub.--1_node.sub.--84 465
HSCOC4_PEA.sub.--1_node.sub.--85 466
HSCOC4_PEA.sub.--1_node.sub.--86 467
HSCOC4_PEA.sub.--1_node.sub.--87 468
HSCOC4_PEA.sub.--1_node.sub.--88 469
HSCOC4_PEA.sub.--1_node.sub.--89 470
HSCOC4_PEA.sub.--1_node.sub.--90 471
HSCOC4_PEA.sub.--1_node.sub.--91 472
HSCOC4_PEA.sub.--1_node.sub.--92 473
HSCOC4_PEA.sub.--1_node.sub.--94 474
HSCOC4_PEA.sub.--1_node.sub.--96 475
HSCOC4_PEA.sub.--1_node.sub.--97 476
HSCOC4_PEA.sub.--1_node.sub.--98 477
HSCOC4_PEA.sub.--1_node.sub.--99 478
HSCOC4_PEA.sub.--1_node.sub.--101 479
HSCOC4_PEA.sub.--1_node.sub.--102 480
HSCOC4_PEA.sub.--1_node.sub.--103 481
HSCOC4_PEA.sub.--1_node.sub.--104 482
HSCOC4_PEA.sub.--1_node.sub.--106 483
HSCOC4_PEA.sub.--1_node.sub.--111 484
[1888] TABLE-US-00690 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) HSCOC4_PEA.sub.--1_P3 488
HSCOC4_PEA.sub.--1_T1 (SEQ ID NO: 387) HSCOC4_PEA.sub.--1_P5 489
HSCOC4_PEA.sub.--1_T3 (SEQ ID NO: 389) HSCOC4_PEA.sub.--1_P6 490
HSCOC4_PEA.sub.--1_T4 (SEQ ID NO: 390) HSCOC4_PEA.sub.--1_P12 491
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO: 394) HSCOC4_PEA.sub.--1_P15 492
HSCOC4_PEA.sub.--1_T14 (SEQ ID NO: 396) HSCOC4_PEA.sub.--1_P16 493
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO: 397) HSCOC4_PEA.sub.--1_P20 494
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO: 398) HSCOC4_PEA.sub.--1_P9 495
HSCOC4_PEA.sub.--1_T21 (SEQ ID NO: 399) HSCOC4_PEA.sub.--1_P22 496
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO: 400) HSCOC4_PEA.sub.--1_P23 497
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO: 401) HSCOC4_PEA.sub.--1_P24 498
HSCOC4_PEA.sub.--1_T30 (SEQ ID NO: 402) HSCOC4_PEA.sub.--1_P25 499
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO: 403) HSCOC4_PEA.sub.--1_P26 500
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO: 404) HSCOC4_PEA.sub.--1_P30 501
HSCOC4_PEA.sub.--1_T40 (SEQ ID NO: 405) HSCOC4_PEA.sub.--1_P38 502
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO: 388) HSCOC4_PEA.sub.--1_P39 503
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO: 391) HSCOC4_PEA.sub.--1_P40 504
HSCOC4_PEA.sub.--1_T7 (SEQ ID NO: 392) HSCOC4_PEA.sub.--1_P41 505
HSCOC4_PEA.sub.--1_T8 (SEQ ID NO: 393) HSCOC4_PEA.sub.--1_P42 506
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO: 395)
[1889] These sequences are variants of the known protein Complement
C4 precursor [Contains: C4a anaphylatoxin] (SwissProt accession
identifier CO4_HUMAN) SEQ ID NO: 485), referred to herein as the
previously known protein.
[1890] Protein Complement C4 precursor [Contains: C4a
anaphylatoxin] (SEQ ID NO:485) is known or believed to have the
following function(s): C4 plays a central role in the activation of
the classical pathway of the complement system. It is processed by
activated C1 which removes from the alpha chain the C4a
anaphylatoxin. Derived from proteolytic degradation of complement
C4, C4a anaphylatoxin is a mediator of local inflammatory process.
It induces the contraction of smooth muscle, increases vascular
permeability and causes histamine release from mast cells and
basophilic leukocytes. The sequence for protein Complement C4
precursor [Contains: C4a anaphylatoxin] (SEQ ID NO:485) is given at
the end of the application, as "Complement C4 precursor [Contains:
C4a anaphylatoxin] (SEQ ID NO:485) amino acid sequence". Known
polymorphisms for this sequence are as shown in Table 4.
TABLE-US-00691 TABLE 4 Amino acid mutations for Known Protein SNP
position(s) on amino acid sequence Comment 477 R->W (in allotype
C4A6). /FTId=VAR.sub.--001987. 726 P->L (in allotype C4A3).
/FTId=VAR.sub.--001988. 1073 D->G (in allotype C4A1, allotype
C4B1 and allotype C4B3). /FTId=VAR.sub.--001989.1120-1125
PCPVLD->LSPVIH (in allotype C4B). /FTId=VAR.sub.--001990.1176
N->S (in allotype C4A1, allotype C4B1, allotype C4B3 and
allotype C4B5). /FTId=VAR.sub.--001991. 1201 S->T (in allotype
C4A6, allotype C4A3, allotype C4A1 and allotype C4B).
/FTId=VAR.sub.--001992.1207 V->A (in allotype C4A1, allotype
C4B1, allotype C4B2 and allotype C4B3). /FTId=VAR.sub.--001993.
1210 L->R (in allotype C4A1, allotype C4B1, allotype C4B2 and
allotype C4B3). /FTId=VAR.sub.--001994. 1286 S->A (in allotype
C4A6, allotype C4A1, allotype C4A3A and allotype C4B).
/FTId=VAR.sub.--001995.1-12 MRLLWGLIWASS->PREVRSVCLSAT 347
S->Y 418 V->A 727 D->N 907 A->T 980-981 VT->LQ 1013
Q->E 1317 I->F 1418-1420 Missing 1654 T->RA
1698H->Q
[1891] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: muscle contraction
regulation; inflammatory response; complement activation;
complement activation, classical pathway, which are annotation(s)
related to Biological Process; complement component; proteinase
inhibitor, which are annotation(s) related to Molecular Function;
and extracellular; extracellular space, which are annotation(s)
related to Cellular Component.
[1892] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nim.nih.gov/projects/LocusLink/.
[1893] Cluster HSCOC4 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 35 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[1894] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 35 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: brain malignant tumors, a mixture of
malignant tumors from different tissues, breast malignant tumors,
pancreas carcinoma and prostate cancer. TABLE-US-00692 TABLE 5
Normal tissue distribution Name of Tissue Number adrenal 853
bladder 328 bone 6 brain 111 colon 245 epithelial 264 general 163
head and neck 0 kidney 141 liver 4109 lung 64 lymph nodes 120
breast 96 bone marrow 0 ovary 116 pancreas 20 prostate 4 stomach 36
T cells 0 Thyroid 12 uterus 127
[1895] TABLE-US-00693 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 5.6e-01
5.9e-01 2.5e-06 0.3 4.3e-04 0.3 bladder 5.0e-01 6.6e-01 6.3e-01 0.9
9.1e-01 0.6 bone 5.5e-01 5.8e-01 1 1.1 7.0e-01 1.3 brain 4.6e-03
6.2e-02 7.7e-11 3.0 3.2e-05 1.7 colon 8.0e-01 8.3e-01 9.8e-01 0.4
9.9e-01 0.4 epithelial 1.7e-01 9.2e-01 9.3e-07 1.3 9.7e-01 0.7
general 3.2e-04 6.1e-01 1.5e-31 2.1 1.9e-03 1.1 head and neck
1.2e-01 2.1e-01 1 1.2 1 1.1 kidney 6.9e-01 8.1e-01 1.2e-04 2.4
1.5e-02 1.5 liver 7.1e-01 7.2e-01 5.0e-04 0.2 1 0.1 lung 2.9e-01
7.1e-01 4.2e-02 1.7 5.1e-01 0.8 lymph nodes 6.3e-01 8.2e-01 9.0e-01
0.5 1 0.3 breast 4.0e-02 1.8e-01 2.1 e-06 6.0 3.9e-03 3.0 bone
marrow 1 6.7e-01 1 1.0 2.8e-01 2.8 ovary 6.6e-01 7.3e-01 1.3e-01
1.5 3.6e-01 1.1 pancreas 1.7e-02 9.9e-02 4.8e-10 7.6 2.9e-07 5.1
prostate 5.8e-01 6.3e-01 4.1e-02 3.9 1.8e-03 3.8 stomach 2.7e-01
7.5e-01 1.1e-01 1.5 6.5e-01 0.8 T cells 1 6.7e-01 1 1.0 7.2e-01 1.4
Thyroid 3.4e-01 3.4e-01 3.0e-01 2.2 3.0e-01 2.2 uterus 1.2e-01
5.3e-01 6.6e-02 1.4 5.4e-01 0.8
[1896] As noted above, cluster HSCOC4 features 19 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Complement C4 precursor
[Contains: C4a anaphylatoxin]. A description of each variant
protein according to the present invention is now provided.
[1897] Variant protein HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1898] Comparison report between HSCOC4_PEA.sub.--1_P3 (SEQ ID
NO:488) and CO4_HUMAN (SEQ ID NO:485):
[1899] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTV corresponding to amino acids
1-865 of CO4_HUMAN (SEQ ID NO:485), which also corresponds to amino
acids 1-865 of HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence RPHRSLSIQELGEPGPSEGWGG (SEQ ID NO:977) corresponding to
amino acids 866-887 of HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[1900] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RPHRSLSIQELGEPGPSEGWGG (SEQ ID NO:977) in HSCOC4_PEA.sub.--1_P3
(SEQ ID NO:488).
[1901] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1902] Variant protein HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 7, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P3 (SEQ ID
NO:488) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00694
TABLE 7 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 869 R->G
Yes
[1903] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 8 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00695 TABLE 8
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 1391 no 862
yes 862 226 yes 226 1328 no
[1904] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 9 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the phosphorylation site is present in the
variant protein; and the last column indicates whether the position
is different on the variant protein). TABLE-US-00696 TABLE 9
Phosphorylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 1420 no 1422
no 1417 no
[1905] Variant protein HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ
ID NO:387), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387) is shown in bold; this coding
portion starts at position 501 and ends at position 3161. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA.sub.--1_P3 (SEQ ID NO:488) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00697 TABLE 10 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 304 A->G Yes 884 G->No 921 C->G
Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T No 1517
C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes 1933
C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes 2322
C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No 2677
C->T Yes 2975 C->T Yes 3105 A->G Yes 3167 G->A Yes 3228
T->C Yes 3259 G->T Yes 3332 G->A Yes 3490 A->C Yes 3569
T->C Yes 3724 G->T Yes 3831 A->G Yes 3898 C->A Yes 3972
C->T Yes 3975 G->C Yes 3983 T->A Yes 3986 G->C Yes 3988
C->T Yes 4140 G->A Yes 4147 T->C Yes 4228 C->G Yes 4233
C->T Yes 4242 G->T Yes 4243 G->C Yes 4339 G->A Yes 4345
C->G Yes 4348 G->A Yes 4469 G->T Yes 4562 A->T Yes 4781
A->G No 4873 T->C Yes 5007 G->No 5423 C->G Yes 5634
G->C No 5677 G->A Yes 5687 A->C Yes 5862 A->C Yes 5868
G->A Yes 5933 A->C Yes
[1906] Variant protein HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T3 (SEQ ID NO:389). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1907] Comparison report between HSCOC4_PEA.sub.--1_P5 (SEQ ID
NO:489) and CO4_HUMAN (SEQ ID NO:485):
[1908] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKG corresponding to
amino acids 1-818 of CO4_HUMAN (SEQ ID NO:485), which also
corresponds to amino acids 1-818 of HSCOC4_PEA.sub.--1_P5 (SEQ ID
NO:489), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID
NO:978) corresponding to amino acids 819-843 of
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1909] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1 P5 (SEQ ID NO:489), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) in HSCOC4_PEA.sub.--1_P5
(SEQ ID NO:489).
[1910] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1911] Variant protein HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P5 (SEQ ID
NO:489) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00698
TABLE 11 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 829 L->P Yes
830 L->I Yes 840 S->P Yes
[1912] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 12 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00699 TABLE 12
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 1391 no 862
no 226 yes 226 1328 no
[1913] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 13 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the phosphorylation site is present in the
variant protein; and the last column indicates whether the position
is different on the variant protein). TABLE-US-00700 TABLE 13
Phosphorylation site(s) Position(s) on known amino acid sequence
Present in variant protein? 1420 no 1422 no 1417 no
[1914] Variant protein HSCOC4_PEA.sub.--1_P5 (SEQ ID NO:489) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T3 (SEQ ID NO:389) is shown in bold; this coding
portion starts at position 501 and ends at position 3029. The
transcript also has the following SNPs as listed in Table 14 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA_I_P5 (SEQ ID NO:489) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00701 TABLE 14 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 304 A->G Yes 884 G->No 921 C->G Yes 1049
C->No 1131 G->No 1465 C->No 1465C->T No 1517C->T Yes
1540 C->A Yes 1768A->No 1778C->T Yes 1933 C->T Yes 1985
C->T Yes 2146 A->C Yes 2162 G->A Yes 2322 C->G Yes 2349
A->G Yes 2435 G->A Yes 2540 C->T No 2677 C->T Yes 2986
T->C Yes 2988 C->A Yes 3018 T->C Yes 3070 C->T Yes 3081
C->A Yes 3093 A->G Yes 3101 G->A Yes 3106 G->A Yes 3174
G->A Yes 3193A->G Yes 3201 T->C Yes 3233 C->T Yes
3363A->G Yes 3425 G->A Yes 3486 T->C Yes 3517 G->T Yes
3590 G->A Yes 3748 A->C Yes 3827 T->C Yes 3982 G->T Yes
4089 A->G Yes 4156 C->A Yes 4230 C->T Yes 4233 G->C Yes
4241 T->A Yes 4244 G->C Yes 4246 C->T Yes 4398 G->A Yes
4405 T->C Yes 4486 C->G Yes 4491 C->T Yes 4500 G->T Yes
4501 G->C Yes 4597 G->A Yes 4603 C->G Yes 4606 G->A Yes
4727 G->T Yes 4820 A->T Yes 5039 A->G No 5131 T->C Yes
5265 G->No 5681 C->G Yes 5892 G->C No 5935 G->A Yes
5945 A->C Yes 6120 A->C Yes 6126 G->A Yes 6191 A->C
Yes
[1915] Variant protein HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1916] Comparison report between HSCOC4_PEA.sub.--1_P6 (SEQ ID
NO:490) and CO4_HUMAN (SEQ ID NO:485):
[1917] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKG corresponding to
amino acids 1-1052 of CO4_HUMAN (SEQ ID NO:485), which also
corresponds to amino acids 1-1052 of HSCOC4_PEA.sub.--1_P6 (SEQ ID
NO:490), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence SGCKGKQEGGQERTVTGRWTAQEATEGKKGGP
(SEQ ID NO:979) corresponding to amino acids 1053-1084 of
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1918] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SGCKGKQEGGQERTVTGRWTAQEATEGKKGGP (SEQ ID NO:979) in
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490).
[1919] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1920] Variant protein HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 15, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P6 (SEQ ID
NO:490) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00702
TABLE 15 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1062 G->V Yes 1068 T->Yes
[1921] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 16 (given according to their position(s) on the
amino acid sequence in the column; the second column indicates
whether the glycosylation site is present in the variant protein;
and the last column indicates whether the position is different on
the variant protein). TABLE-US-00703 TABLE 16 Glycosylation site(s)
Position(s) on known amino Present in acid sequence variant
protein? Position in variant protein? 1391 no 862 yes 862 226 yes
226 1328 no
[1922] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1 P6 (SEQ ID NO:490), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 17 (given according to their position(s) on the
amino acid sequence in the column; the second column indicates
whether the phosphorylation site is present in the variant protein;
and the last column indicates whether the position is different on
the variant protein). TABLE-US-00704 TABLE 17 Phosphorylation
site(s) Position(s) on known amino acid sequence Present in variant
protein? 1420 no 1422 no 1417 no
[1923] Variant protein HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T4 (SEQ
ID NO:390), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390) is shown in bold; this coding
portion starts at position 501 and ends at position 3752. The
transcript also has the following SNPs as listed in Table 18 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA.sub.--1_P6 (SEQ ID NO:490) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00705 TABLE 18 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 304 A->G Yes 884 G->No 921 C->G Yes
1049 C->No 1131 G->No 1465 C->No 1465 C->T No 1517
C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes 1933
C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes 2322
C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No 2677
C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes 3219
G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes 3685
G->T Yes 3702 A->Yes 3897 A->G Yes 3964 C->A Yes 4038
C->T Yes 4041 G->C Yes 4049 T->A Yes 4052 G->C Yes 4054
C->T Yes 4206 G->A Yes 4213 T->C Yes 4294 C->G Yes 4299
C->T Yes 4308 G->T Yes 4309 G->C Yes 4405 G->A Yes 4411
C->G Yes 4414 G->A Yes 4535 G->T Yes 4628 A->T Yes 4847
A->G No 4939 T->C Yes 5073 G->No 5489 C->G Yes 5700
G->C No 5743 G->A Yes 5753 A->C Yes 5928 A->C Yes 5934
G->A Yes 5999 A->C Yes
[1924] Variant protein HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1925] Comparison report between HSCOC4_PEA.sub.--1_P12 (SEQ ID
NO:491) and CO4_HUMAN_V1 (SEQ ID NO: 486):
[1926] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NRSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKV corresponding to amino acids 1-1380
of CO4_HUMAN_V1 (SEQ ID NO:486), which also corresponds to amino
acids 1-1380 of HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence RAREGVGPGTGGGEGVE (SEQ ID NO:980) corresponding to amino
acids 1381-1397 of HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[1927] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RAREGVGPGTGGGEGVE (SEQ ID NO:980) in HSCOC4_PEA.sub.--1_P12 (SEQ ID
NO:491).
[1928] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00706 TABLE 19 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1929] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1930] Variant protein HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 20, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P12 (SEQ ID
NO:491) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00707
TABLE 20 Amino acid mutations SNP position(s) on amino Alternative
amino acid sequence acid(s) Previously known SNP? 128 Q->No 141
L->V Yes 183 G->No 211 G->No 322 A->No 322 A->V No
347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P Yes 608
L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes 907
A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes 1121
C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes 1207
A->V Yes 1210 R->L Yes 1286 A->S Yes 1317 1->F Yes
[1931] Variant protein HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T11 (SEQ
ID NO:394), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394) is shown in bold; this
coding portion starts at position 501 and ends at position 4691.
The transcript also has the following SNPs as listed in Table 21
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P12 (SEQ ID NO:491) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00708 TABLE 21
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4859 C->T Yes 4876 C->A Yes
4882 C->G Yes 4924 G->A Yes 5205 C->G Yes 5596 C->T Yes
5717 A->G No 5809 T->C Yes 5943 G->No 6359 C->G Yes
6570 G->C No 6613 G->A Yes 6623 A->C Yes 6798 A->C Yes
6804 G->A Yes 6869 A->C Yes
[1932] Variant protein HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1933] Comparison report between HSCOC4_PEA.sub.--1_P15 (SEQ ID
NO:492) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1934] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK.
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRMRVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQ corresponding to amino acids 1-1359 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1359 of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VNHSLVNHSLAWVARTPGPRGQARSRPQPPTRGIPAALLPGVFGGRLTSWLRDLEL (SEQ ID
NO:981) corresponding to amino acids 1360-1415 of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492, wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1935] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VNHSLVNHSLAWVARTPGPRGQARSRPQPPTRGIPAALLPGVFGGRLTSWLRDLEL (SEQ ID
NO:981) in HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492).
[1936] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00709 TABLE 22 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1937] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1938] Variant protein HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 23, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P15 (SEQ ID
NO:492) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00710
TABLE 23 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->V No
322 A->No 347 S->Y Yes 423 Q->No 478 P->L Yes
549H->P Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872
V->A Yes 907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120
P->L Yes 1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176
S->N Yes 1207A->V Yes 1210 R->L Yes 1286A->S Yes 1317
1->F Yes 1387 Q->H Yes 1411 R->C Yes
[1939] Variant protein HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T14 (SEQ
ID NO:396), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396) is shown in bold; this
coding portion starts at position 501 and ends at position 4745.
The transcript also has the following SNPs as listed in Table 24
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P15 (SEQ ID NO:492) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00711 TABLE 24
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4661 A->C Yes 4731 C->T Yes
4872 A->G Yes 4905 C->T Yes 5061 A->G No 5153 T->C Yes
5287 G->No 5703 C->G Yes 5914 G->C No 5957 G->A Yes
5967 A->C Yes 6142 A->C Yes 6148 G->A Yes 6213 A->C
Yes
[1940] Variant protein HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1941] Comparison report between HSCOC4_PEA.sub.--1_P16 (SEQ ID
NO:493) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1942] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPK corresponding to
amino acids 1-1457 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1-1457 of HSCOC4_PEA.sub.--1_P16 (SEQ ID
NO:493), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence AERQGGAVWHGHRGRHPPEWIPRPAC (SEQ ID
NO:982) corresponding to amino acids 1458-1483 of
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1943] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
AERQGGAVWHGHRGRHPPEWIPRPAC (SEQ ID NO:982) in
HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493).
[1944] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00712 TABLE 25 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1945] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because of manual inspection of known
protein localization and/or gene structure.
[1946] Variant protein HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 26, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P16 (SEQ ID
NO:493) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00713
TABLE 26 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes
1207 A->V Yes 1210 R->L Yes 1286 A->S Yes 1317 1->F Yes
1390 K->E No
[1947] Variant protein HSCOC4_PEA.sub.--1_P16 (SEQ ID NO:493) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T15 (SEQ
ID NO:397), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397) is shown in bold; this
coding portion starts at position 501 and ends at position 4949.
The transcript also has the following SNPs as listed in Table 27
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA_I_P16 (SEQ ID NO:493) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00714 TABLE 27 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 304 A->G Yes 884 G->No 921 C->G Yes
1049 C->No 1131 G->No 1465 C->No 1465 C->T No 1517
C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes 1933
C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes 2322
C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No 2677
C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes 3219
G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes 3718
A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes 3870
T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes 4034
T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes 4130
G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes 4356
G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes 5263
C->G Yes 5474 G->C No 5517 G->A Yes 5527 A->C Yes 5702
A->C Yes 5708 G->A Yes 5773 A->C Yes
[1948] Variant protein HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1949] Comparison report between HSCOC4_PEA.sub.--1_P20 (SEQ ID
NO:494) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1950] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQ corresponding to amino acids 1-1303 of CO4_HUMAN_V1
(SEQ ID NO:486), which also corresponds to amino acids 1-1303 of
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
VGAVPGLWRGWVVLRPRACLSPGSTSLGHGDCPGCPVCLLDCLPHH (SEQ ID NO:983)
corresponding to amino acids 1304-1349 of HSCOC4_PEA.sub.--1_P20
(SEQ ID NO:494), wherein said first amino acid sequence and second
amino acid sequence are contiguous and in a sequential order.
[1951] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00715 (SEQ ID NO:983)
VGAVPGLWRGWVVLRPRACLSPGSTSLGHGDCPGCPVCLLDCLPHH in
HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494).
[1952] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00716 TABLE 28 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1953] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1954] Variant protein HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 29, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P20 (SEQ ID
NO:494) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00717
TABLE 29 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes
1207 A->V Yes 1210 R->L Yes 1286 A->S Yes 1312 R->G Yes
1344 D->V Yes
[1955] Variant protein HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398) is shown in bold; this
coding portion starts at position 501 and ends at position 4547.
The transcript also has the following SNPs as listed in Table 30
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P20 (SEQ ID NO:494) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00718 TABLE 30
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4434 C->G Yes 4531 A->T Yes 4743 A->C Yes
4813 C->T Yes 4954 A->G Yes 4987 C->T Yes 5143 A->G No
5235 T->C Yes 5369 G->No 5785 C->G Yes 5996 G->C No
6039 G->A Yes 6049 A->C Yes 6224 A->C Yes 6230 G->A Yes
6295 A->C Yes
[1956] Variant protein HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to, the present
invention to each such aligned protein is as follows:
[1957] Comparison report between HSCOC4_PEA.sub.--1_P9 (SEQ ID
NO:495) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1958] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDSV
corresponding to amino acids 1-1529 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1529 of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence SGER (SEQ
ID NO:984) corresponding to amino acids 1530-1533 of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1959] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence SGER (SEQ
ID NO:984) in HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495).
[1960] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00719 TABLE 31 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1961] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1962] Variant protein HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 32, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P9 (SEQ ID
NO:495) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00720
TABLE 32 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s)
[1963] Previously known SNP? 128 Q->No 141 L->V Yes 183
G->No 211 G->No 322 A->V No 322 A->No 347 S->Y Yes
423 Q->No 478 P->L Yes 549H->P Yes 608 L->V Yes 617
K->E Yes 726 P->L Yes 872 V->A Yes 907 A->T Yes 959
E->D Yes 1073 D->G Yes 1120 P->L Yes 1121 C->S Yes 1124
L->I Yes 1125 D->H Yes 1176 S->N Yes 1207 A->V Yes 1210
R->L Yes 1286A->S Yes 1317 1->F Yes 1390 K->E No 1465
R->No
[1964] Variant protein HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495) is
encoded by the following transcript(s): HSCOC4_PEA1_T21 (SEQ ID
NO:399), for which the sequence(s) is/are given at the end of the
application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399) is shown in bold; this
coding portion starts at position 501 and ends at position 5099.
The transcript also has the following SNPs as listed in Table 33
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P9 (SEQ ID NO:495) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00721 TABLE 33
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465C->T
No 1517 C->T Yes 1540C->A Yes 1768A->No 1778C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894 G->No 5561 G->A Yes 6026 T->G Yes 6348 G->C Yes
6966 C->G Yes 7177 G->C No 7220 G->A Yes 7230 A->C Yes
7405 A->C Yes 7411 G->A Yes 7476 A->C Yes
[1965] Variant protein HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1966] Comparison report between HSCOC4_PEA.sub.--1_P22 (SEQ ID
NO:496) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1967] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LASLVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAF
RLFETKITQVLHF corresponding to amino acids 1-1653 of CO4_HUMAN_V1
(SEQ ID NO:486), which also corresponds to amino acids 1-1653 of
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SMKQTGEAGRAGGRQGG (SEQ ID NO:985) corresponding to amino acids
1654-1670 of HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[1968] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SMKQTGEAGRAGGRQGG (SEQ ID NO:985) in HSCOC4_PEA.sub.--1_P22 (SEQ ID
NO:496).
[1969] It should be noted that the known protein sequence
(C04_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00722 TABLE 34 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1970] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1971] Variant protein HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 35, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P22 (SEQ ID
NO:496) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00723
TABLE 35 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124L->I Yes 1125D->H Yes 1176S->N Yes
1207A->V Yes 1210R->L Yes 1286 A->S Yes 1317 1->F Yes
1390 K->E No 1465 R->No 1604 R->G Yes
[1972] Variant protein HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T25 (SEQ
ID NO:400), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400) is shown in bold; this
coding portion starts at position 501 and ends at position 5510.
The transcript also has the following SNPs as listed in Table 36
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P22 (SEQ ID NO:496) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00724 TABLE 36
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894 G->No 5310 C->G Yes 5783 G->C No 5826 G->A Yes
5836 A->C Yes 5974 C->T Yes 5981 C->T Yes 6154 A->C Yes
6160 G->A Yes 6225 A->C Yes 6283 C->T Yes 6548 C->T Yes
6567 C->T Yes 7300 C->A Yes 7520 C->T Yes 7685 A->C
Yes
[1973] Variant protein HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1974] Comparison report between HSCOC4_PEA.sub.--1_P23 (SEQ ID
NO:497) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1975] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKANEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLMSRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYG corresponding to amino
acids 1-1626 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1-1626 of HSCOC4_PEA.sub.--1_P23 (SEQ ID
NO:497), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
QSSHRGPGLTLPRGPAVLVSLGVACSSYRSCTQPVCSDTNFLPSQPQSNSPFPLLLTPS (SEQ ID
NO:986) corresponding to amino acids 1627-1685 of
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1976] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00725 (SEQ ID NO:986)
QSSHRGPGLTLPRGPAVLVSLGVACSSYRSCTQPVCSDTNFLPSQPQSNSPFPLLLTPS in
HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497).
[1977] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00726 TABLE 37 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1978] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because of manual inspection of known
protein localization and/or gene structure.
[1979] Variant protein HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 38, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P23 (SEQ ID
NO:497) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00727
TABLE 38 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->V No
322 A->No 347 S->Y Yes 423 Q->No 478 P->L Yes
549H->P Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872
V->A Yes 907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120
P->L Yes 1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176
S->N Yes 1207 A->V Yes 1210 R->L Yes 1286A->S Yes 1317
1->F Yes 1390 K->E No 1465 R->No 1604 R->G Yes 1634
G->Yes
[1980] Variant protein HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401) is shown in bold; this
coding portion starts at position 501 and ends at position 5555.
The transcript also has the following SNPs as listed in Table 39
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P23 (SEQ ID NO:497) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00728 TABLE 39
Nucleic acid SNPs SNP position on nucleotide sequence Alternative
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894G->No 5310 C->G Yes 5402 C->Yes 5426 T->C Yes 5965
G->C No 6008 G->A Yes 6018 A->C Yes 6156 C->T Yes 6163
C->T Yes 6336 A->C Yes 6342 G->A Yes 6407 A->C Yes 6465
C->T Yes 6730 C->T Yes 6749 C->T Yes 7482 C->A Yes 7702
C->T Yes 7867 A->C Yes
[1981] Variant protein HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1982] Comparison report between HSCOC4_PEA.sub.--1_P24 (SEQ ID
NO:498) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1983] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDS
corresponding to amino acids 1-1528 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1528 of
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SADVLCFTGHQVRADSWPPCVLLKSASVLRGSALASVAPWSGVCRTRMATG (SEQ ID NO:987)
corresponding to amino acids 1529-1579 of HSCOC4_PEA.sub.--1_P24
(SEQ ID NO:498), wherein said first amino acid sequence and second
amino acid sequence are contiguous and in a sequential order.
[1984] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00729 (SEQ ID NO:987)
SADVLCFTGHQVRADSWPPCVLLKSASVLRGSALASVAPWSGVCRTRMATG in
HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498).
[1985] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00730 TABLE 40 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1986] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1987] Variant protein HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 41, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P24 (SEQ ID
NO:498) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00731
TABLE 41 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes
1207A->V Yes 1210 R->L Yes 1286 A->S Yes 1317 1->F Yes
1390 K->E No 1465 R->No 1569 S->R Yes
[1988] Variant protein HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T30 (SEQ
ID NO:402), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402) is shown in bold; this
coding portion starts at position 501 and ends at position 5237.
The transcript also has the following SNPs as listed in Table 42
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P24 (SEQ ID NO:498) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00732 TABLE 42
Nucleic acid SNPs SNP position on nucleotide sequence Alternative
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894 G->No 5207 C->G Yes 5418 G->C No 5461 G->A Yes
5471 A->C Yes 5646 A->C Yes 5652 G->A Yes 5717 A->C
Yes
[1989] Variant protein HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1990] Comparison report between HSCOC4_PEA.sub.--1_P25 (SEQ ID
NO:499) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1991] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEG corresponding to amino acids 1-1593 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1593 of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDEVCLLLPPC GVRLPG
(SEQ ID NO:988) corresponding to amino acids 1594-1657 of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[1992] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499) comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00733 (SEQ ID NO:988)
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDE VCLLLPPCGVRLPG
in HSCOC4_PEA.sub.--1_P25 (SEQ ID NO: 499).
[1993] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00734 TABLE 43 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[1994] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[1995] Variant protein HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 44, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P25 (SEQ ID
NO:499) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00735
TABLE 44 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes
1207 A->V Yes 1210 R->L Yes 1286 A->S Yes 1317 1->F Yes
1390 K->E No 1465 R->No 1632A->G Yes
[1996] Variant protein HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T31 (SEQ
ID NO:403), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) is shown in bold; this
coding portion starts at position 501 and ends at position 5471.
The transcript also has the following SNPs as listed in Table 45
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P25 (SEQ ID NO:499) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00736 TABLE 45
Nucleic acid SNPs SNP position on nucleotide sequence Alternative
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668A->G No 4760 T->C Yes
4894 G->No 5395 C->G Yes 5606 G->C No 5649 G->A Yes
5659 A->C Yes 5834 A->C Yes 5840 G->A Yes 5905 A->C
Yes
[1997] Variant protein HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[1998] Comparison report between HSCOC4_PEA.sub.--1_P26 (SEQ ID
NO:500) and CO4_HUMAN_V1 (SEQ ID NO:486):
[1999] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL
YFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLC
SAEVCQCAEG corresponding to amino acids 1-1593 of CO4_HUMAN_V1 (SEQ
ID NO:486), which also corresponds to amino acids 1-1593 of
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
TABLE-US-00737 (SEQ ID NO:989)
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDE
VCLLLPPCGVRSVFPPRPWPDPPSGTGCFGLSGCSLLLLQVMHAACLL corresponding to
amino acids 1594-1691 of HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[2000] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00738 (SEQ ID NO:989)
ETEGLGRGSGGGMAGAPPTLSDGFPNFREVPSPASRPGAGSAGRGWLQDE
VCLLLPPCGVRSVFPPRPWPDPPSGTGCFGLSGCSLLLLQVMHAACLL in
HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500).
[2001] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00739 TABLE 46 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[2002] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2003] Variant protein HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 47, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P26 (SEQ ID
NO:500) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00740
TABLE 47 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176 S->N Yes
1207 A->V Yes 1210 R->L Yes 1286 A->S Yes 1317 1->F Yes
1390 K->E No 1465 R->No 1632 A->G Yes 1663 P->Yes 1671
C->R Yes
[2004] Variant protein HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404), for which the sequencers) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404) is shown in bold; this
coding portion starts at position 501 and ends at position 5573.
The transcript also has the following SNPs as listed in Table 48
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P26 (SEQ ID NO:500) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00741 TABLE 48
Nucleic acid SNPs SNP position on nucleotide sequence Alternative
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894 G->No 5395 C->G Yes 5487 C->Yes 5511 T->C Yes 6050
G->C No 6093 G->A Yes 6103 A->C Yes 6278 A->C Yes 6284
G->A Yes 6349 A->C Yes 6407 C->T Yes 6672 C->T Yes 6691
C->T Yes 7424 C->A Yes 7644 C->T Yes 7809 A->C Yes
[2005] Variant protein HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2006] Comparison report between HSCOC4_PEA.sub.--1_P30 (SEQ ID
NO:501) and CO4_HUMAN_V3 (SEQ ID NO: 487):
[2007] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYMWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGS
corresponding to amino acids 1-1232 of CO4_HUMAN_V3 (SEQ ID
NO:487), which also corresponds to amino acids 1-1232 of
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
RNPVRLLQPRAQMFCVLRGTK (SEQ ID NO:990) corresponding to amino acids
1233-1253 of HSCOC4_PEA.sub.--1.sub.P30 (SEQ ID NO:501), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2008] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RNPVRLLQPRAQMFCVLRGTK (SEQ ID NO:990) in HSCOC4_PEA.sub.--1_P30
(SEQ ID NO:501).
[2009] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN (SEQ ID NO:485)_V3 (SEQ ID
NO:487). These changes were previously known to occur and are
listed in the table below. TABLE-US-00742 TABLE 49 Changes to
CO4_HUMAN (SEQ ID NO: 485) _V3 (SEQ ID NO: 487) SNP position(s) on
amino acid sequence Type of change 1177 variant 1202 variant 1208
variant 1211 variant
[2010] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region program predicts that this protein
has a trans-membrane region.
[2011] Variant protein HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 50, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P30 (SEQ ID
NO:501) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00743
TABLE 50 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872 V->A Yes
907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120 P->L Yes
1121 C->S Yes 1124L->I Yes 1125D->H Yes 1176S->N Yes
1207 A->V Yes 1210R->L Yes
[2012] Variant protein HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T40 (SEQ
ID NO:405), for which the sequencers) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405) is shown in bold; this
coding portion starts at position 501 and ends at position 4259.
The transcript also has the following SNPs as listed in Table 51
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P30 (SEQ ID NO:501) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00744 TABLE 51
Nucleic acid SNPs SNP position on nucleotide sequence Alternative
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377A->C Yes 3456 T->C Yes 3611 G->T Yes
3718A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4348 C->G Yes 4559 G->C No 4602 G->A Yes
4612 A->C Yes 4787 A->C Yes 4793 G->A Yes 4858 A->C
Yes
[2013] Variant protein HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2014] Comparison report between HSCOC4_PEA.sub.--1_P38 (SEQ ID
NO:502) and CO4_HUMAN (SEQ ID NO:485):
[2015] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVMPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKG corresponding to
amino acids 1-818 of CO4_HUMAN (SEQ ID NO:485), which also
corresponds to amino acids 1-818 of HSCOC4_PEA.sub.--1_P38 (SEQ ID
NO:502), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID
NO:978) corresponding to amino acids 819-843 of
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2016] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502) comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DVTLSGPQVTLLPFPCTPAPCSLCS (SEQ ID NO:978) in HSCOC4_PEA.sub.--1_P38
(SEQ ID NO:502).
[2017] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2018] Variant protein HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 52, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P38 (SEQ ID
NO:502) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00745
TABLE 52 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes 423 Q->No 478 P->L Yes 549H->P
Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 829 L->P Yes
830 L->I Yes 840 S->P Yes
[2019] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 53 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00746 TABLE 53
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 1391 no 862
no 226 yes 226 1328 no
[2020] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 54 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the phosphorylation site is present in the
variant protein; and the last column indicates whether the position
is different on the variant protein). TABLE-US-00747 TABLE 54
Phosphorylation site(s) Position(s) on known amino acid sequence
Present in variant protein? 1420 no 1422 no 1417 no
[2021] Variant protein HSCOC4_PEA.sub.--1_P38 (SEQ ID NO:502) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T2 (SEQ
ID NO:388), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388) is shown in bold; this coding
portion starts at position 501 and ends at position 3029. The
transcript also has the following SNPs as listed in Table 55 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA1_P38 (SEQ ID NO:502) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00748 TABLE 55 Nucleic acid SNPs SNP
position on nucleotide sequence Alternative nucleic acid Previously
known SNP? 304 A->G Yes 884 G->No 921 C->G Yes 1049
C->No 1131 G->No 1465 C->No 1465 C->T No 1517 C->T
Yes 1540 C->A Yes 1768A->No 1778 C->T Yes 1933 C->T Yes
1985 C->T Yes 2146 A->C Yes 2162 G->A Yes 2322 C->G Yes
2349 A->G Yes 2435 G->A Yes 2540 C->T No 2677 C->T Yes
2986 T->C Yes 2988 C->A Yes 3018 T->C Yes 3070 C->T Yes
3081 C->A Yes 3093 A->G Yes 3101 G->A Yes 3106 G->A Yes
3174 G->A Yes 3193 A->G Yes 3201 T->C Yes 3233 C->T Yes
3373 T->C Yes 3404 G->T Yes 3477 G->A Yes 3635 A->C Yes
3714 T->C Yes 3869 G->T Yes 3976 A->G Yes 4043 C->A Yes
4117 C->T Yes 4120 G->C Yes 4128 T->A Yes 4131 G->C Yes
4133 C->T Yes 4285 G->A Yes 4292 T->C Yes 4373 C->G Yes
4378 C->T Yes 4387 G->T Yes 4388 G->C Yes 4484 G->A Yes
4490 C->G Yes 4493 G->A Yes 4614 G->T Yes 4707 A->T Yes
4926 A->G No 5018 T->C Yes 5152 G->No 5568 C->G Yes
5779 G->C No 5822 G->A Yes 5832 A->C Yes 6007 A->C Yes
6013 G->A Yes 6078 A->C Yes
[2022] Variant protein HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2023] Comparison report between HSCOC4_PEA.sub.--1_P39 (SEQ ID
NO:503) and CO4_HUMAN (SEQ ID NO:485):
[2024] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQ corresponding to amino acids 1-387
of CO4_HUMAN (SEQ ID NO:485), which also corresponds to amino acids
1-387 of HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VSSRGEG (SEQ ID NO:992) corresponding to amino acids
388-394 of HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2025] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence VSSRGEG
(SEQ ID NO:992) in HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503).
[2026] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2027] Variant protein HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 56, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P39 (SEQ ID
NO:503) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00749
TABLE 56 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->No 322
A->V No 347 S->Y Yes
[2028] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 57 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00750 TABLE 57
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 1391 no 862
no 226 yes 226 1328 no
[2029] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 58 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the phosphorylation site is present in the
variant protein; and the last column indicates whether the position
is different on the variant protein). TABLE-US-00751 TABLE 58
Phosphorylation site(s) Position(s) on known amino acid sequence
Present in variant protein? 1420 no 1422 no 1417 no
[2030] Variant protein HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T5 (SEQ
ID NO:391), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391) is shown in bold; this coding
portion starts at position 501 and ends at position 1682. The
transcript also has the following SNPs as listed in Table 59 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA.sub.--1_P39 (SEQ ID NO:503) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00752 TABLE 59 Nucleic acid
SNPs SNP position on nucleotide sequence Alternative nucleic acid
Previously known SNP? 304 A->G Yes 884 G->No 921 C->G Yes
1049 C->No 1131 G->No 1465 C->No 1465 C->T No 1517
C->T Yes 1540 C->A Yes 1742 C->A Yes 1756 C->A Yes 1867
A->No 1877 C->T Yes 2032 C->T Yes 2084 C->T Yes 2245
A->C Yes 2261 G->A Yes 2421 C->G Yes 2448 A->G Yes 2534
G->A Yes 2639 C->T No 2776 C->T Yes 3074 C->T Yes 3214
T->C Yes 3245 G->T Yes 3318 G->A Yes 3476 A->C Yes 3555
T->C Yes 3710 G->T Yes 3817 A->G Yes 3884 C->A Yes 3958
C->T Yes 3961 G->C Yes 3969 T->A Yes 3972 G->C Yes 3974
C->T Yes 4126 G->A Yes 4133 T->C Yes 4214 C->G Yes 4219
C->T Yes 4228 G->T Yes 4229 G->C Yes 4325 G->A Yes 4331
C->G Yes 4334 G->A Yes 4455 G->T Yes 4548 A->T Yes 4767
A->G No 4859 T->C Yes 4993 G->No 5409 C->G Yes 5620
G->C No 5663 G->A Yes 5673 A->C Yes 5848 A->C Yes 5854
G->A Yes 5919 A->C Yes
[2031] Variant protein HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T7 (SEQ ID NO:392). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2032] Comparison report between HSCOC4_PEA.sub.--1_P40 (SEQ ID
NO:504) and CO4_HUMAN (SEQ ID NO:485):
[2033] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKY
corresponding to amino acids 1-236 of CO4_HUMAN (SEQ ID NO:485),
which also corresponds to amino acids 1-236 of
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
AGEWTEPHFPLKGRVPGRPGEAEYGHY (SEQ ID NO:993) corresponding to amino
acids 237-263 of HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2034] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
AGEWTEPHFPLKGRVPGRPGEAEYGHY (SEQ ID NO:993) in
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504).
[2035] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2036] Variant protein HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 60, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P40 (SEQ ID
NO:504) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00753
TABLE 60 Amino acid mutations SNP position(s) on amino acid
sequence Alternative amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 254 R->No
[2037] The glycosylation sites of variant protein
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 61 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00754 TABLE 61
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 1391 no 862
no 226 yes 226 1328 no
[2038] The phosphorylation sites of variant protein
HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504), as compared to the known
protein Complement C4 precursor [Contains: C4a anaphylatoxin], are
described in Table 62 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the phosphorylation site is present in the
variant protein; and the last column indicates whether the position
is different on the variant protein). TABLE-US-00755 TABLE 62
Phosphorylation site(s) Position(s) on known amino acid sequence
Present in variant protein? 1420 no 1422 no 1417 no
[2039] Variant protein HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T7 (SEQ ID NO:392) is shown in bold; this coding
portion starts at position 501 and ends at position 1289. The
transcript also has the following SNPs as listed in Table 63 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA.sub.--1_P40 (SEQ ID NO:504) sequence provides
support for the deduced sequence variant protein according to the
present invention). TABLE-US-00756 TABLE 63 Nucleic acid SNPs SNP
position on nucleotide Alternative sequence nucleic acid Previously
known SNP? 304 A->G Yes 884 G->No 921 C->G Yes 1049
C->No 1131 G->No 1262 C->No 1262 C->T No 1314 C->T
Yes 1337 C->A Yes 1565A->No 1575 C->T Yes 1730 C->T Yes
1782 C->T Yes 1943 A->C Yes 1959 G->A Yes 2119 C->G Yes
2146 A->G Yes 2232 G->A Yes 2337 C->T No 2474 C->T Yes
2772 C->T Yes 2912 T->C Yes 2943 G->T Yes 3016 G->A Yes
3174 A->C Yes 3253 T->C Yes 3408 G->T Yes 3515 A->G Yes
3582 C->A Yes 3656 C->T Yes 3659 G->C Yes 3667 T->A Yes
3670 G->C Yes 3672 C->T Yes 3824 G->A Yes 3831 T->C Yes
3912 C->G Yes 3917 C->T Yes 3926 G->T Yes 3927 G->C Yes
4023 G->A Yes 4029 C->G Yes 4032 G->A Yes 4153 G->T Yes
4246 A->T Yes 4465 A->G No 4557 T->C Yes 4691 G->No
5107 C->G Yes 5318 G->C No 5361 G->A Yes 5371 A->C Yes
5546 A->C Yes 5552 G->A Yes 5617 A->C Yes
[2040] Variant protein HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393). An alignment is given to the
known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2041] Comparison report between HSCOC4_PEA.sub.--1_P41 (SEQ ID
NO:505) and CO4_HUMAN_V1 (SEQ ID NO:486):
[2042] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSWPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV
HYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLL YFDSV
corresponding to amino acids 1-1529 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1529 of
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence SGER (SEQ
ID NO:984) corresponding to amino acids 1530-1533 of
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2043] 2. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence SGER (SEQ
ID NO:984) in HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505).
[2044] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00757 TABLE 64 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[2045] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because of manual inspection of known
protein localization and/or gene structure.
[2046] Variant protein HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 65, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P41 (SEQ ID
NO:505) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00758
TABLE 65 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->V No
322 A->No 347 S->Y Yes 423 Q->No 478 P->L Yes
549H->P Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872
V->A Yes 907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120
P->L Yes 1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176
S->N Yes 1207 A->V Yes 1210 R->L Yes 1286A->S Yes 1317
1->F Yes 1390 K->E No 1465 R->No
[2047] Variant protein HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T8 (SEQ
ID NO:393), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393) is shown in bold; this coding
portion starts at position 501 and ends at position 5099. The
transcript also has the following SNPs as listed in Table 66 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSCOC4_PEA.sub.--1_P41 (SEQ ID NO:505) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00759 TABLE 66 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 304 A->G Yes 884 G->No 921 C->G Yes
1049 C->No 1131 G->No 1465 C->No 1465 C->T No 1517
C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes 1933
C->T Yes 1985 C->T Yes 2146A->C Yes 2162 G->A Yes 2322
C->G Yes 2349A->G Yes 2435 G->A Yes 2540 C->T No 2677
C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes 3219
G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes 3718
A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes 3870
T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes 4034
T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes 4130
G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes 4356
G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes 4894
G->No 5561 G->A Yes 6026 T->G Yes 6348 G->C Yes 6801
C->G Yes 7012 G->C No 7055 G->A Yes 7065 A->C Yes 7240
A->C Yes 7246 G->A Yes 7311 A->C Yes
[2048] Variant protein HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSCOC4_PEA.sub.--1_T1 2 (SEQ ID NO:395). An alignment is given to
the known protein (Complement C4 precursor [Contains: C4a
anaphylatoxin]) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2049] Comparison report between HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506) and CO4_HUMAN_V1 (SEQ ID NO:486):
[2050] 1. An isolated chimeric polypeptide encoding for
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), comprising a first amino
acid sequence being at least 90% homologous to
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLR
NPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLK
DSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMV
ENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVL
PNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFR
GLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAE
LTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVP
EVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPD
SRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLA
PSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDS
LALVALGALDTALYMGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAG
LAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAKRCCQDGVTR
LPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLID
EDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQL
RVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQ
QVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREEL
VYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASL
LRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRK
ADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQ
DPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKASS
FLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTR
QGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVE
YTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKWEEQESRV HYTVCIW
corresponding to amino acids 1-1473 of CO4_HUMAN_V1 (SEQ ID
NO:486), which also corresponds to amino acids 1-1473 of
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
WAPGAALGQGREGRTQAGAGLLEPAQAEPGRQLTRLHR (SEQ ID NO:1021)
corresponding to amino acids 1474-1511 of HSCOC4_PEA.sub.--1_P42
(SEQ ID NO:506), a third amino acid sequence being at least 90%
homologous to RNGKVGLSGMAIADVTLLSGFHALRADLEK corresponding to amino
acids 1474-1503 of CO4_HUMAN_V1 (SEQ ID NO:486), which also
corresponds to amino acids 1512-1541 of HSCOC4_PEA.sub.--1_P42 (SEQ
ID NO:506), and a fourth amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence VWSATQGNPLCPRY (SEQ ID NO:995)
corresponding to amino acids 1542-1555 of HSCOC4_PEA.sub.--1_P42
(SEQ ID NO:506), wherein said first amino acid sequence, second
amino acid sequence, third amino acid sequence and fourth amino
acid sequence are contiguous and in a sequential order.
[2051] 2. An isolated polypeptide encoding for an edge portion of
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), comprising an amino acid
sequence being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
encoding for WAPGAALGQGREGRTQAGAGLLEPAQAEPGRQLTRLHR (SEQ ID NO:
1021), corresponding to HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506).
[2052] 3. An isolated polypeptide encoding for a tail of
HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
VWSATQGNPLCPRY (SEQ ID NO:995) in HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506).
[2053] It should be noted that the known protein sequence
(CO4_HUMAN (SEQ ID NO:485)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for CO4_HUMAN_V1 (SEQ ID NO:486). These changes
were previously known to occur and are listed in the table below.
TABLE-US-00760 TABLE 67 Changes to CO4_HUMAN_V1 (SEQ ID NO: 486)
SNP position(s) on amino acid sequence Type of change 1177 variant
1202 variant 1208 variant 1211 variant 1287 variant
[2054] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2055] Variant protein HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 68, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506) sequence provides support for the deduced sequence s
variant protein according to the present invention). TABLE-US-00761
TABLE 68 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 128
Q->No 141 L->V Yes 183 G->No 211 G->No 322 A->V No
322 A->No 347 S->Y Yes 423 Q->No 478 P->L Yes
549H->P Yes 608 L->V Yes 617 K->E Yes 726 P->L Yes 872
V->A Yes 907 A->T Yes 959 E->D Yes 1073 D->G Yes 1120
P->L Yes 1121 C->S Yes 1124 L->I Yes 1125 D->H Yes 1176
S->N Yes 1207 A->V Yes 1210 R->L Yes 1286A->S Yes 1317
1->F Yes 1390 K->E No 1465 R->No
[2056] Variant protein HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506) is
encoded by the following transcript(s): HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395) is shown in bold; this
coding portion starts at position 501 and ends at position 5165.
The transcript also has the following SNPs as listed in Table 69
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSCOC4_PEA.sub.--1_P42 (SEQ ID NO:506) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00762 TABLE 69
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 304 A->G Yes 884 G->No 921
C->G Yes 1049 C->No 1131 G->No 1465 C->No 1465 C->T
No 1517 C->T Yes 1540 C->A Yes 1768 A->No 1778 C->T Yes
1933 C->T Yes 1985 C->T Yes 2146 A->C Yes 2162 G->A Yes
2322 C->G Yes 2349 A->G Yes 2435 G->A Yes 2540 C->T No
2677 C->T Yes 2975 C->T Yes 3115 T->C Yes 3146 G->T Yes
3219 G->A Yes 3377 A->C Yes 3456 T->C Yes 3611 G->T Yes
3718 A->G Yes 3785 C->A Yes 3859 C->T Yes 3862 G->C Yes
3870 T->A Yes 3873 G->C Yes 3875 C->T Yes 4027 G->A Yes
4034 T->C Yes 4115 C->G Yes 4120 C->T Yes 4129 G->T Yes
4130 G->C Yes 4226 G->A Yes 4232 C->G Yes 4235 G->A Yes
4356 G->T Yes 4449 A->T Yes 4668 A->G No 4760 T->C Yes
4894 G->No 5765 G->A Yes 6230 T->G Yes 6552 G->C Yes
7005 C->G Yes 7216 G->C No 7259 G->A Yes 7269 A->C Yes
7444 A->C Yes 7450 G->A Yes 7515 A->C Yes
[2057] As noted above, cluster HSCOC4 features 79 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[2058] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--1 (SEQ ID
NO:406) according to the present invention is supported by 24
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 70
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00763 TABLE 70 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1 535 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1 535 NO: 388) HSCOC4_PEA 1 T3 (SEQ
ID 1 535 NO: 389) HSCOC4_PEA.sub.--1 T4 (SEQ ID 1 535 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1 535 NO: 391) HSCOC4_PEA.sub.--1_T7
(SEQ ID 1 535 NO: 392) HSCOC4_PEA.sub.--1_T8 (SEQ ID 1 535 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1 535 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1 535 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1 535 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1 535 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1 535 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1 535 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1 535 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1 535 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1 535 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1 535 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1 535 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1 535 NO: 405)
[2059] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--5 (SEQ ID
NO:407) according to the present invention is supported by 29
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 71
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00764 TABLE 71 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 566 764 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 566 764 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 566 764 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 566 764 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 566 764 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 566 764 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 566 764 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 566 764 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 566 764 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 566 764 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 566 764 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 566 764 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 566 764 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 566 764 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 566 764 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 566 764 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 566 764 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 566 764 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 566 764 NO: 405)
[2060] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:408) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 72
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00765 TABLE 72 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 765 885 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 765 885 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 765 885 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 765 885 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 765 885 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 765 885 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 765 885 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 765 885 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 765 885 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 765 885 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 765 885 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 765 885 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 765 885 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 765 885 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 765 885 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 765 885 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 765 885 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 765 885 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 765 885 NO: 405)
[2061] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--30 (SEQ ID
NO:409) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4.sub.PEA.sub.--1_T3
(SEQ ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11(SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 73
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00766 TABLE 73 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1662 1841 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1662 1841 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1662 1841 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1662 1841 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1761 1940 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1459 1638 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1662 1841 NO: 393) HSCOC4_PEA 1 T11
(SEQ ID 1662 1841 NO: 394) HSCOC4_PEA.sub.--1_T12 (SEQ ID 1662 1841
NO: 395) HSCOC4_PEA.sub.--1_T14 (SEQ ID 1662 1841 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1662 1841 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1662 1841 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1662 1841 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1662 1841 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1662 1841 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1662 1841 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1662 1841 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1662 1841 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1662 1841 NO: 405)
[2062] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--33 (SEQ ID
NO:410) according to the present invention is supported by 30
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1 T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 74
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00767 TABLE 74 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1842 2024 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1842 2024 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1842 2024 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1842 2024 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1941 2123 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1639 1821 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1842 2024 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1842 2024 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1842 2024 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1842 2024 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1842 2024 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1842 2024 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1842 2024 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1842 2024 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1842 2024 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1842 2024 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1842 2024 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1842 2024 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1842 2024 NO: 405)
[2063] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--35 (SEQ ID
NO:411) according to the present invention is supported by 31
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 75
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00768 TABLE 75 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2025 2210 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2025 2210 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2025 2210 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2025 2210 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2124 2309 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1822 2007 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2025 2210 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2025 2210 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2025 2210 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2025 2210 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2025 2210 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2025 2210 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2025 2210 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2025 2210 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2025 2210 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2025 2210 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2025 2210 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2025 2210 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2025 2210 NO: 405)
[2064] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--37 (SEQ ID
NO:412) according to the present invention is supported by 33
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 76
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00769 TABLE 76 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2211 2369 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2211 2369 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2211 2369 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2211 2369 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2310 2468 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2008 2166 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2211 2369 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2211 2369 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2211 2369 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2211 2369 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2211 2369 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2211 2369 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2211 2369 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2211 2369 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2211 2369 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2211 2369 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2211 2369 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2211 2369 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2211 2369 NO: 405)
[2065] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--39 (SEQ ID
NO:413) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 77
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00770 TABLE 77 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2370 2496 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2370 2496 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2370 2496 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2370 2496 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2469 2595 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2167 2293 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2370 2496 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2370 2496 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2370 2496 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2370 2496 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2370 2496 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2370 2496 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2370 2496 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2370 2496 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2370 2496 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2370 2496 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2370 2496 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2370 2496 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2370 2496 NO: 405)
[2066] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--43 (SEQ ID
NO:414) according to the present invention is supported by 34
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1.sub.--T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3
(SEQ ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1.sub.-T12
(SEQ ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 78
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00771 TABLE 78 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2572 2769 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2572 2769 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2572 2769 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2572 2769 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2671 2868 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2369 2566 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2572 2769 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2572 2769 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2572 2769 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2572 2769 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2572 2769 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2572 2769 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2572 2769 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2572 2769 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2572 2769 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2572 2769 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2572 2769 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2572 2769 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2572 2769 NO: 405)
[2067] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--48 (SEQ ID
NO:415) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388) and
HSCOC4_PEA.sub.--1_T3 (SEQ ID NO:389). Table 79 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00772 TABLE 79 Segment location on transcripts Segment
Segment Transcript name starting position ending position
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2953 3210 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2953 3210 NO: 389)
[2068] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--49 (SEQ ID
NO:416) according to the present invention is supported by 37
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 80
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00773 TABLE 80 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2953 3092 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3211 3350 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3211 3350 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2953 3092 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3052 3191 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2750 2889 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2953 3092 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2953 3092 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2953 3092 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2953 3092 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2953 3092 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2953 3092 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2953 3092 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2953 3092 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2953 3092 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2953 3092 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2953 3092 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2953 3092 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2953 3092 NO: 405)
[2069] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--51 (SEQ ID
NO:417) according to the present invention is supported by 40
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 81
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00774 TABLE 81 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3206 3415 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3351 3560 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3464 3673 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3093 3302 NO: 390) HSCOC4_PEA 1 T5
(SEQ ID 3192 3401 NO: 391) HSCOC4_PEA.sub.--1_T7 (SEQ ID 2890 3099
NO: 392) HSCOC4_PEA.sub.--1_T8 (SEQ ID 3093 3302 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3093 3302 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3093 3302 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3093 3302 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3093 3302 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3093 3302 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3093 3302 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3093 3302 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3093 3302 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3093 3302 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3093 3302 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3093 3302 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3093 3302 NO: 405)
[2070] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--58 (SEQ ID
NO:418) according to the present invention is supported by 52
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 82
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00775 TABLE 82 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3605 3767 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3750 3912 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3863 4025 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3492 3654 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3591 3753 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3289 3451 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3492 3654 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3492 3654 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3492 3654 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3492 3654 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3492 3654 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3492 3654 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3492 3654 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3492 3654 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3492 3654 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3492 3654 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3492 3654 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3492 3654 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3492 3654 NO: 405)
[2071] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--59 (SEQ ID
NO:419) according to the present invention is supported by 8
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390). Table 83
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00776 TABLE 83 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T4 (SEQ ID 3655 3833 NO:
390)
[2072] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--62 (SEQ ID
NO:420) according to the present invention is supported by 61
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1.sub.--T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3
(SEQ ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1 T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1 T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 84
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00777 TABLE 84 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3844 4000 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3989 4145 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4102 4258 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3910 4066 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3830 3986 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3528 3684 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3731 3887 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3731 3887 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3731 3887 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3731 3887 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3731 3887 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3731 3887 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3731 3887 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3731 3887 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3731 3887 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3731 3887 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3731 3887 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3731 3887 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3731 3887 NO: 405)
[2073] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--66 (SEQ ID
NO:421) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 85
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00778 TABLE 85 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 4118 4289 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 4263 4434 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4376 4547 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4184 4355 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4104 4275 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3802 3973 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4005 4176 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4005 4176 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4005 4176 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4005 4176 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4005 4176 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4005 4176 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4005 4176 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4005 4176 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4005 4176 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4005 4176 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4005 4176 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4005 4176 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4005 4176 NO: 405)
[2074] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--72 (SEQ ID
NO:422) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 86 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00779 TABLE
86 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4392 4522 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4537 4667 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4650 4780 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4458 4588 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4378 4508 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4076 4206 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4279 4409 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4279 4409 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4279 4409 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4279 4409 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4279 4409 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4279 4409 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4279 4409 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4279 4409 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4279 4409 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4279 4409 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4279 4409 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4279 4409 NO: 404)
[2075] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--77 (SEQ ID
NO:423) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396) and
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398). Table 87 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00780 TABLE 87 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4578 4970 NO: 396)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4660 5052 NO: 398)
[2076] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--79 (SEQ ID
NO:424) according to the present invention is supported by 6
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394). Table 88
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00781 TABLE 88 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T11 (SEQ ID 4638 5686 NO:
394)
[2077] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--93 (SEQ ID
NO:425) according to the present invention is supported by 25
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395) and HSCOC4_PEA.sub.--1_T21
(SEQ ID NO:399). Table 89 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00782 TABLE
89 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T8 (SEQ ID
5085 6566 NO: 393) HSCOC4_PEA.sub.--1_T12 (SEQ ID 5289 6770 NO:
395) HSCOC4_PEA.sub.--1_T21 (SEQ ID 5085 6566 NO: 399)
[2078] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--100 (SEQ ID
NO:426) according to the present invention is supported by 13
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399). Table 90
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00783 TABLE 90 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T21 (SEQ ID 6679 6843 NO:
399)
[2079] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--105 (SEQ ID
NO:427) according to the present invention is supported by 9
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 91 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00784 TABLE 91 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5377 5558 NO: 401)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5462 5643 NO: 404)
[2080] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (with
regard to breast cancer), shown in Table 92. TABLE-US-00785 TABLE
92 Oligonucleotides related to this segment Chip Oligonucleotide
name Overexpressed in cancers reference
HSCOC4.sub.--0.sub.--0.sub.--9883 (SEQ ID breast malignant tumors
BRS NO: 909)
[2081] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--107 (SEQ ID
NO:428) according to the present invention is supported by 27
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400),
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 93 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00786 TABLE
93 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T25 (SEQ ID
5461 5722 NO: 400) HSCOC4_PEA.sub.--1_T28 (SEQ ID 5643 5904 NO:
401) HSCOC4_PEA.sub.--1_T32 (SEQ ID 5728 5989 NO: 404)
[2082] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--108 (SEQ ID
NO:429) according to the present invention is supported by 120
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 94
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00787 TABLE 94 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5574 5706 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5719 5851 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5832 5964 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5640 5772 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5560 5692 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5258 5390 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6952 7084 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6510 6642 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7156 7288 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5854 5986 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5414 5546 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5936 6068 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 7117 7249 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5723 5855 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5905 6037 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5358 5490 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5546 5678 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5990 6122 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4499 4631 NO: 405)
[2083] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--109 (SEQ ID
NO:430) according to the present invention is supported by 12
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400) and
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401). Table 95 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00788 TABLE 95 Segment location on transcripts
Segment Segment Transcript name starting position ending position
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5856 5998 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 6038 6180 NO: 401)
[2084] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--110 (SEQ ID
NO:431) according to the present invention is supported by 97
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1 T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 96
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00789 TABLE 96 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5707 5856 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5852 6001 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5965 6114 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5773 5922 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5693 5842 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5391 5540 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 7085 7234 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6643 6792 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7289 7438 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5987 6136 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5547 5696 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 6069 6218 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 7250 7399 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5999 6148 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 6181 6330 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5491 5640 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5679 5828 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 6123 6272 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4632 4781 NO: 405)
[2085] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--112 (SEQ ID
NO:432) according to the present invention is supported by 71
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 97
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00790 TABLE 97 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA 1 T1 (SEQ ID 5948 5989 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 6093 6134 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 6206 6247 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 6014 6055 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5934 5975 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5632 5673 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 7326 7367 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6884 6925 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7530 7571 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 6228 6269 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5788 5829 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 6310 6351 NO: 398) HSCOC4_PEA 1 T21
(SEQ ID 7491 7532 NO: 399) HSCOC4_PEA.sub.--1_T25 (SEQ ID 6240 6619
NO: 400) HSCOC4_PEA.sub.--1_T28 (SEQ ID 6422 6801 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5732 5773 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5920 5961 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 6364 6743 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4873 4914 NO: 405)
[2086] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--113 (SEQ ID
NO:433) according to the present invention is supported by 19
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400),
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 98 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00791 TABLE
98 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T25 (SEQ ID
6620 7765 NO: 400) HSCOC4_PEA.sub.--1_T28 (SEQ ID 6802 7947 NO:
401) HSCOC4_PEA.sub.--1_T32 (SEQ ID 6744 7889 NO: 404)
[2087] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2088] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:434) according to the present invention is supported by 25
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 99
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00792 TABLE 99 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 536 565 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 536 565 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 536 565 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 536 565 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 536 565 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 536 565 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 536 565 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 536 565 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 536 565 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 536 565 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 536 565 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 536 565 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 536 565 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 536 565 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 536 565 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 536 565 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 536 565 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 536 565 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 536 565 NO: 405)
[2089] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:435) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 100
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00793 TABLE 100 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 886 966 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 886 966 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 886 966 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 886 966 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 886 966 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 886 966 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 886 966 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 886 966 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 886 966 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 886 966 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 886 966 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 886 966 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 886 966 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 886 966 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 886 966 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 886 966 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 886 966 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 886 966 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 886 966 NO: 405)
[2090] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--10 (SEQ ID
NO:436) according to the present invention is supported by 33
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 101
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00794 TABLE 101 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 967 1037 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 967 1037 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 967 1037 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 967 1037 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 967 1037 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 967 1037 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 967 1037 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 967 1037 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 967 1037 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 967 1037 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 967 1037 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 967 1037 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 967 1037 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 967 1037 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 967 1037 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 967 1037 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 967 1037 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 967 1037 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 967 1037 NO: 405)
[2091] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:437) according to the present invention is supported by 33
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 102
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00795 TABLE 102 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1038 1126 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1038 1126 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1038 1126 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1038 1126 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1038 1126 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1038 1126 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1038 1126 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1038 1126 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1038 1126 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1038 1126 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1038 1126 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1038 1126 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1038 1126 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1038 1126 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1038 1126 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1038 1126 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1038 1126 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1038 1126 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1038 1126 NO: 405)
[2092] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:438) according to the present invention is supported by 30
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 103
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00796 TABLE 103 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1127 1209 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1127 1209 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1127 1209 NO: 389) HSCOC4_PEA.sub.--1
T4 (SEQ ID 1127 1209 NO: 390) HSCOC4_PEA.sub.--1_T5 (SEQ ID 1127
1209 NO: 391) HSCOC4_PEA.sub.--1_T7 (SEQ ID 1127 1209 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1127 1209 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1127 1209 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1127 1209 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1127 1209 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1127 1209 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1127 1209 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1127 1209 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1127 1209 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1127 1209 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1127 1209 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1127 1209 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1127 1209 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1127 1209 NO: 405)
[2093] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:439) according to the present invention is supported by 28
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T8 (SEQ
ID NO:393), HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394),
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ
ID NO:396), HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397),
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ
ID NO:399), HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400),
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ
ID NO:402), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403),
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404) and HSCOC4_PEA.sub.--1_T40
(SEQ ID NO:405). Table 104 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00797 TABLE
104 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
1210 1306 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 1210 1306 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1210 1306 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1210 1306 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1210 1306 NO: 391)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1210 1306 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1210 1306 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1210 1306 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1210 1306 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1210 1306 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1210 1306 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1210 1306 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1210 1306 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1210 1306 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1210 1306 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1210 1306 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1210 1306 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1210 1306 NO: 405)
[2094] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:440) according to the present invention is supported by 27
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1.sub.--T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1 T3
(SEQ ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T8 (SEQ
ID NO:393), HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394),
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ
ID NO:396), HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397),
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ
ID NO:399), HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400),
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ
ID NO:402), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403),
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404) and HSCOC4_PEA.sub.--1_T40
(SEQ ID NO:405). Table 105 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00798 TABLE
105 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
1307 1412 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 1307 1412 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1307 1412 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1307 1412 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1307 1412 NO: 391)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1307 1412 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1307 1412 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1307 1412 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1307 1412 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1307 1412 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1307 1412 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1307 1412 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1307 1412 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1307 1412 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1307 1412 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1307 1412 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1307 1412 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1307 1412 NO: 405)
[2095] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:441) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA-1_T3 (SEQ ID
NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1 T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 106
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00799 TABLE 106 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1413 1439 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1413 1439 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1413 1439 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1413 1439 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1413 1439 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1210 1236 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1413 1439 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1413 1439 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1413 1439 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1413 1439 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1413 1439 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1413 1439 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1413 1439 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1413 1439 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1413 1439 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1413 1439 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1413 1439 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1413 1439 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1413 1439 NO: 405)
[2096] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:442) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 107
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00800 TABLE 107 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1440 1545 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1440 1545 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1440 1545 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1440 1545 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1440 1545 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1237 1342 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1440 1545 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1440 1545 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1440 1545 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1440 1545 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1440 1545 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1440 1545 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1440 1545 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1440 1545 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1440 1545 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1440 1545 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1440 1545 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1440 1545 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1440 1545 NO: 405)
[2097] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--28 (SEQ ID
NO:443) according to the present invention is supported by 34
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 108
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00801 TABLE 108 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 1546 1661 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 1546 1661 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 1546 1661 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 1546 1661 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 1546 1661 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 1343 1458 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 1546 1661 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 1546 1661 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 1546 1661 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 1546 1661 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 1546 1661 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 1546 1661 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 1546 1661 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 1546 1661 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 1546 1661 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 1546 1661 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 1546 1661 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 1546 1661 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 1546 1661 NO: 405)
[2098] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:444) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391). Table 109
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00802 TABLE 109 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T5 (SEQ ID 1662 1760 NO:
391)
[2099] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--41 (SEQ ID
NO:445) according to the present invention is supported by 32
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 110
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00803 TABLE 110 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2497 2571 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2497 2571 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2497 2571 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2497 2571 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2596 2670 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2294 2368 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2497 2571 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2497 2571 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2497 2571 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2497 2571 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2497 2571 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2497 2571 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2497 2571 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2497 2571 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2497 2571 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2497 2571 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2497 2571 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2497 2571 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2497 2571 NO: 405)
[2100] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--45 (SEQ ID
NO:446) according to the present invention is supported by 31
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 111
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00804 TABLE 111 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2770 2881 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2770 2881 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2770 2881 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2770 2881 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2869 2980 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2567 2678 NO: 392) HSCOC4_PEA 1 T8
(SEQ ID 2770 2881 NO: 393) HSCOC4_PEA.sub.--1_T11 (SEQ ID 2770 2881
NO: 394) HSCOC4_PEA.sub.--1_T12 (SEQ ID 2770 2881 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2770 2881 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2770 2881 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2770 2881 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2770 2881 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2770 2881 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2770 2881 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2770 2881 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2770 2881 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2770 2881 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2770 2881 NO: 405)
[2101] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--47 (SEQ ID
NO:447) according to the present invention is supported by 32
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 112
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00805 TABLE 112 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 2882 2952 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 2882 2952 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 2882 2952 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 2882 2952 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 2981 3051 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 2679 2749 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 2882 2952 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 2882 2952 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 2882 2952 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 2882 2952 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 2882 2952 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 2882 2952 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 2882 2952 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 2882 2952 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 2882 2952 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 2882 2952 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 2882 2952 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 2882 2952 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 2882 2952 NO: 405)
[2102] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--50 (SEQ ID
NO:448) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387) and
HSCOC4_PEA.sub.--1_T3 (SEQ ID NO:389). Table 113 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00806 TABLE 113 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3093 3205 NO: 387)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3351 3463 NO: 389)
[2103] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--53 (SEQ ID
NO:449) according to the present invention is supported by 38
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 114
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00807 TABLE 114 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3416 3467 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3561 3612 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3674 3725 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3303 3354 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3402 3453 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3100 3151 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3303 3354 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3303 3354 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3303 3354 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3303 3354 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3303 3354 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3303 3354 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3303 3354 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3303 3354 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3303 3354 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3303 3354 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3303 3354 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3303 3354 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3303 3354 NO: 405)
[2104] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--55 (SEQ ID
NO:450) according to the present invention is supported by 40
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 115
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00808 TABLE 115 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3468 3557 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3613 3702 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3726 3815 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3355 3444 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3454 3543 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3152 3241 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3355 3444 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3355 3444 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3355 3444 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3355 3444 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3355 3444 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3355 3444 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3355 3444 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3355 3444 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3355 3444 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3355 3444 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3355 3444 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3355 3444 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3355 3444 NO: 405)
[2105] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--57 (SEQ ID
NO:451) according to the present invention is supported by 42
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 116
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00809 TABLE 116 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3558 3604 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3703 3749 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 3816 3862 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3445 3491 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3544 3590 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3242 3288 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3445 3491 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3445 3491 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3445 3491 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3445 3491 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3445 3491 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3445 3491 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3445 3491 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3445 3491 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3445 3491 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3445 3491 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3445 3491 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3445 3491 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3445 3491 NO: 405)
[2106] Segment cluster HSCOC4_PEA.sub.--1-node.sub.--60 (SEQ ID
NO:452) according to the present invention is supported by 50
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 117
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00810 TABLE 117 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 3768 3843 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 3913 3988 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4026 4101 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 3834 3909 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3754 3829 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3452 3527 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3655 3730 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3655 3730 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3655 3730 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3655 3730 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3655 3730 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3655 3730 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3655 3730 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3655 3730 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3655 3730 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3655 3730 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3655 3730 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3655 3730 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3655 3730 NO: 405)
[2107] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--64 (SEQ ID
NO:453) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 118
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00811 TABLE 118 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 4001 4117 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 4146 4262 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4259 4375 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4067 4183 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 3987 4103 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3685 3801 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 3888 4004 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 3888 4004 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 3888 4004 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 3888 4004 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 3888 4004 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 3888 4004 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 3888 4004 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 3888 4004 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 3888 4004 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 3888 4004 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 3888 4004 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 3888 4004 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 3888 4004 NO: 405)
[2108] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--69 (SEQ ID
NO:454) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 119
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00812 TABLE 119 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 4290 4309 NO: 387)
HSCOC4_PEA 1 T2 (SEQ ID 4435 4454 NO: 388). HSCOC4_PEA.sub.--1_T3
(SEQ ID 4548 4567 NO: 389) HSCOC4_PEA.sub.--1_T4 (SEQ ID 4356 4375
NO: 390) HSCOC4_PEA.sub.--1_T5 (SEQ ID 4276 4295 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3974 3993 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4177 4196 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4177 4196 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4177 4196 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4177 4196 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4177 4196 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4177 4196 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4177 4196 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4177 4196 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4177 4196 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4177 4196 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4177 4196 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4177 4196 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4177 4196 NO: 405)
[2109] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--70 (SEQ ID
NO:455) according to the present invention is supported by 58
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 120 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00813 TABLE
120 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4310 4349 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4455 4494 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4568 4607 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4376 4415 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4296 4335 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 3994 4033 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4197 4236 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4197 4236 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4197 4236 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4197 4236 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4197 4236 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4197 4236 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4197 4236 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4197 4236 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4197 4236 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4197 4236 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4197 4236 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4197 4236 NO: 404)
[2110] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--71 (SEQ ID
NO:456) according to the present invention is supported by 58
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1-T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 121 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00814 TABLE
121 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4350 4391 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4495 4536 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4608 4649 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4416 4457 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4336 4377 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4034 4075 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4237 4278 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4237 4278 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4237 4278 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4237 4278 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4237 4278 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4237 4278 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4237 4278 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4237 4278 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4237 4278 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4237 4278 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4237 4278 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4237 4278 NO: 404)
[2111] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--73 (SEQ ID
NO:457) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398). Table 122
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00815 TABLE 122 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T20 (SEQ ID 4410 4491 NO:
398)
[2112] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--74 (SEQ ID
NO:458) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 123 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00816 TABLE
123 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4523 4546 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4668 4691 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4781 4804 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4589 4612 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4509 4532 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4207 4230 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4410 4433 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4410 4433 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4410 4433 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4410 4433 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4410 4433 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4492 4515 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4410 4433 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4410 4433 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4410 4433 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4410 4433 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4410 4433 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4410 4433 NO: 404)
[2113] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--75 (SEQ ID
NO:459) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 124 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00817 TABLE
124 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4547 4626 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4692 4771 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4805 4884 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4613 4692 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4533 4612 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4231 4310 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4434 4513 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4434 4513 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4434 4513 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4434 4513 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4434 4513 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4516 4595 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4434 4513 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4434 4513 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4434 4513 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4434 4513 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4434 4513 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4434 4513 NO: 404)
[2114] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--76 (SEQ ID
NO:460) according to the present invention is supported by 66
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1 T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1 T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1 T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1 T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1 T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 125 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00818 TABLE
125 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4627 4690 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4772 4835 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4885 4948 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4693 4756 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4613 4676 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4311 4374 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4514 4577 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4514 4577 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4514 4577 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4514 4577 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4514 4577 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 4596 4659 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4514 4577 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4514 4577 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4514 4577 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4514 4577 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4514 4577 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4514 4577 NO: 404)
[2115] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--78 (SEQ ID
NO:461) according to the present invention is supported by 71
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 126 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00819 TABLE
126 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4691 4750 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4836 4895 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 4949 5008 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4757 4816 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4677 4736 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4375 4434 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4578 4637 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 4578 4637 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4578 4637 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 4971 5030 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4578 4637 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5053 5112 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4578 4637 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4578 4637 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4578 4637 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4578 4637 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4578 4637 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4578 4637 NO: 404)
[2116] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--80 (SEQ ID
NO:462) according to the present invention is supported by 75
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390), HSCOC4_PEA-1_T5
(SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ ID NO:392),
HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393), HSCOC4_PEA.sub.--1_T11 (SEQ
ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395),
HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396), HSCOC4_PEA.sub.--1_T15 (SEQ
ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398),
HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399), HSCOC4_PEA.sub.--1_T25 (SEQ
ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401),
HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402), HSCOC4_PEA.sub.--1_T31 (SEQ
ID NO:403) and HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 127
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00820 TABLE 127 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 4751 4844 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 4896 4989 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5009 5102 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4817 4910 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4737 4830 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4435 4528 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4638 4731 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5687 5780 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4638 4731 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5031 5124 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4638 4731 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5113 5206 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4638 4731 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4638 4731 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4638 4731 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4638 4731 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4638 4731 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4638 4731 NO: 404)
[2117] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--82 (SEQ ID
NO:463) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 128 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00821 TABLE
128 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4845 4855 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 4990 5000 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5103 5113 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4911 4921 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4831 4841 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4529 4539 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4732 4742 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5781 5791 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4732 4742 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5125 5135 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4732 4742 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5207 5217 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4732 4742 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4732 4742 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4732 4742 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4732 4742 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4732 4742 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4732 4742 NO: 404)
[2118] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--83 (SEQ ID
NO:464) according to the present invention is supported by 77
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 129 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00822 TABLE
129 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4856 4971 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5001 5116 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5114 5229 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 4922 5037 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4842 4957 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4540 4655 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4743 4858 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5792 5907 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4743 4858 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5136 5251 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4743 4858 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5218 5333 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4743 4858 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4743 4858 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4743 4858 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4743 4858 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4743 4858 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4743 4858 NO: 404)
[2119] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--84 (SEQ ID
NO:465) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 130 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00823 TABLE
130 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
4972 4984 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5117 5129 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5230 5242 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5038 5050 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4958 4970 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4656 4668 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4859 4871 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5908 5920 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4859 4871 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5252 5264 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4859 4871 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5334 5346 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4859 4871 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4859 4871 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4859 4871 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4859 4871 NO: 402)
HSCOC4_PEA.sub.--1 T31 (SEQ ID 4859 4871 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4859 4871 NO: 404)
[2120] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--85 (SEQ ID
NO:466) according to the present invention is supported by 68
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T20 (SEQ ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ
ID NO:399), HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400),
HSCOC4_PEA.sub.--1_T28 (SEQ ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ
ID NO:402), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 131 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00824 TABLE 131 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 4985 5031 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5130 5176 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5243 5289 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5051 5097 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 4971 5017 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4669 4715 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4872 4918 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5921 5967 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 4872 4918 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5265 5311 NO: 396)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5347 5393 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4872 4918 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4872 4918 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4872 4918 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4872 4918 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4872 4918 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4872 4918 NO: 404)
[2121] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--86 (SEQ ID
NO:467) according to the present invention is supported by 7
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395). Table 132
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00825 TABLE 132 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T12 (SEQ ID 4919 5032 NO:
395)
[2122] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--87 (SEQ ID
NO:468) according to the present invention is supported by 74
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.-1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 133 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00826 TABLE
133 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5032 5122 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5177 5267 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5290 5380 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5098 5188 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5018 5108 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4716 4806 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 4919 5009 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 5968 6058 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 5033 5123 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5312 5402 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4872 4962 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5394 5484 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 4919 5009 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 4919 5009 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 4919 5009 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 4919 5009 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 4919 5009 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 4919 5009 NO: 404)
[2123] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--88 (SEQ ID
NO:469) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395). Table 134
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00827 TABLE 134 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T12 (SEQ ID 5124 5213 NO:
395)
[2124] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--89 (SEQ ID
NO:470) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1=T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 135 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00828 TABLE
135 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5123 5131 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5268 5276 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5381 5389 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5189 5197 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5109 5117 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4807 4815 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 5010 5018 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6059 6067 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 5214 5222 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5403 5411 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4963 4971 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5485 5493 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 5010 5018 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5010 5018 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5010 5018 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5010 5018 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5010 5018 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5010 5018 NO: 404)
[2125] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--90 (SEQ ID
NO:471) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1 T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1 T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 136 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00829 TABLE
136 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5132 5142 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5277 5287 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5390 5400 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5198 5208 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5118 5128 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4816 4826 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 5019 5029 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6068 6078 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 5223 5233 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5412 5422 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4972 4982 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5494 5504 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 5019 5029 NO: 399) HSCOC4_PEA 1 T25
(SEQ ID 5019 5029 NO: 400) HSCOC4_PEA.sub.--1_T28 (SEQ ID 5019 5029
NO: 401) HSCOC4_PEA.sub.--1_T30 (SEQ ID 5019 5029 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5019 5029 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5019 5029 NO: 404)
[2126] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--91 (SEQ ID
NO:472) according to the present invention is supported by 78
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 137 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00830 TABLE
137 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5143 5179 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5288 5324 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5401 5437 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5209 5245 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5129 5165 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4827 4863 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 5030 5066 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6079 6115 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 5234 5270 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5423 5459 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 4983 5019 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5505 5541 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 5030 5066 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5030 5066 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5030 5066 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5030 5066 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5030 5066 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5030 5066 NO: 404)
[2127] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--92 (SEQ ID
NO:473) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and HSCOC4_PEA.sub.--1_T32
(SEQ ID NO:404). Table 138 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00831 TABLE
138 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5180 5197 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5325 5342 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5438 5455 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5246 5263 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5166 5183 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4864 4881 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 5067 5084 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6116 6133 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 5271 5288 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5460 5477 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5020 5037 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5542 5559 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 5067 5084 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5067 5084 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5067 5084 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5067 5084 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5067 5084 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5067 5084 NO: 404)
[2128] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--94 (SEQ ID
NO:474) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T12 (SEQ ID NO:395) and HSCOC4_PEA.sub.--1_T21
(SEQ ID NO:399). Table 139 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00832 TABLE
139 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T8 (SEQ ID
6567 6575 NO: 393) HSCOC4_PEA.sub.--1_T12 (SEQ ID 6771 6779 NO:
395) HSCOC4_PEA.sub.--1_T21 (SEQ ID 6567 6575 NO: 399)
[2129] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--96 (SEQ ID
NO:475) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 140 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00833 TABLE 140 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5198 5205 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5343 5350 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5456 5463 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5264 5271 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5184 5191 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4882 4889 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6576 6583 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6134 6141 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 6780 6787 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5478 5485 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5038 5045 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5560 5567 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 6576 6583 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5085 5092 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5085 5092 NO: 401)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5085 5092 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5085 5092 NO: 404)
[2130] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--97 (SEQ ID
NO:476) according to the present invention can be found in the
following transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 141 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00834 TABLE 141 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5206 5222 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5351 5367 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5464 5480 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5272 5288 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5192 5208 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4890 4906 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6584 6600 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6142 6158 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 6788 6804 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5486 5502 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5046 5062 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5568 5584 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 6584 6600 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5093 5109 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5093 5109 NO: 401)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5093 5109 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5093 5109 NO: 404)
[2131] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--98 (SEQ ID
NO:477) according to the present invention is supported by 93
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 142 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00835 TABLE 142 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5223 5271 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5368 5416 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5481 5529 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5289 5337 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5209 5257 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4907 4955 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6601 6649 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6159 6207 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 6805 6853 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5503 5551 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5063 5111 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5585 5633 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 6601 6649 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5110 5158 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5110 5158 NO: 401)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5110 5158 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5110 5158 NO: 404)
[2132] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--99 (SEQ ID
NO:478) according to the present invention is supported by 93
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403),
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404) and HSCOC4_PEA.sub.--1_T40
(SEQ ID NO:405). Table 143 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00836 TABLE
143 Segment location on transcripts Segment Segment Transcript name
starting position ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID
5272 5300 NO: 387) HSCOC4_PEA.sub.--1_T2 (SEQ ID 5417 5445 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5530 5558 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5338 5366 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5258 5286 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4956 4984 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6650 6678 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6208 6236 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 6854 6882 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5552 5580 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5112 5140 NO: 397) HSCOC4_PEA_lT20
(SEQ ID 5634 5662 NO: 398) HSCOC4_PEA.sub.--1_T21 (SEQ ID 6650 6678
NO: 399) HSCOC4_PEA.sub.--1_T25 (SEQ ID 5159 5187 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5159 5187 NO: 401)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5159 5187 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5159 5187 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4197 4225 NO: 405)
[2133] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--101 (SEQ ID
NO:479) according to the present invention is supported by 116
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 144
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00837 TABLE 144 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5301 5390 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5446 5535 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5559 5648 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5367 5456 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5287 5376 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 4985 5074 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6679 6768 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6237 6326 NO: 394) HSCOC4
PEA.sub.--1_T12 (SEQ ID 6883 6972 NO: 395) HSCOC4_PEA.sub.--1_T14
(SEQ ID 5581 5670 NO: 396) HSCOC4_PEA.sub.--1_T15 (SEQ ID 5141 5230
NO: 397) HSCOC4_PEA.sub.--1_T20 (SEQ ID 5663 5752 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 6844 6933 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5188 5277 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5188 5277 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5085 5174 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5188 5277 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5188 5277 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4226 4315 NO: 405)
[2134] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--102 (SEQ ID
NO:480) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403) and
HSCOC4_PEA.sub.--1_T32 (SEQ ID NO:404). Table 145 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00838 TABLE 145 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T31 (SEQ ID 5278 5362 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5278 5362 NO: 404)
[2135] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--103 (SEQ ID
NO:481) according to the present invention is supported by 106
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 146
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00839 TABLE 146 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5391 5463 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5536 5608 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5649 5721 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5457 5529 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5377 5449 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5075 5147 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6769 6841 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6327 6399 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 6973 7045 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5671 5743 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5231 5303 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5753 5825 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 6934 7006 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5278 5350 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5278 5350 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5175 5247 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5363 5435 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5363 5435 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4316 4388 NO: 405)
[2136] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--104 (SEQ ID
NO:482) according to the present invention is supported by 101
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1.sub.T3
(SEQ ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1 T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 147
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00840 TABLE 147 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5464 5489 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5609 5634 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5722 5747 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5530 5555 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5450 5475 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5148 5173 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6842 6867 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6400 6425 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7046 7071 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5744 5769 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5304 5329 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5826 5851 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 7007 7032 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5351 5376 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5351 5376 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5248 5273 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5436 5461 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5436 5461 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4389 4414 NO: 405)
[2137] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--106 (SEQ ID
NO:483) according to the present invention is supported by 110
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 148
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00841 TABLE 148 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5490 5573 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 5635 5718 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 5748 5831 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5556 5639 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5476 5559 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5174 5257 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 6868 6951 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6426 6509 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7072 7155 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 5770 5853 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5330 5413 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 5852 5935 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 7033 7116 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 5377 5460 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 5559 5642 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5274 5357 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5462 5545 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 5644 5727 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4415 4498 NO: 405)
[2138] Segment cluster HSCOC4_PEA.sub.--1_node.sub.--111 (SEQ ID
NO:484) according to the present invention is supported by 77
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSCOC4_PEA.sub.--1_T1 (SEQ ID NO:387),
HSCOC4_PEA.sub.--1_T2 (SEQ ID NO:388), HSCOC4_PEA.sub.--1_T3 (SEQ
ID NO:389), HSCOC4_PEA.sub.--1_T4 (SEQ ID NO:390),
HSCOC4_PEA.sub.--1_T5 (SEQ ID NO:391), HSCOC4_PEA.sub.--1_T7 (SEQ
ID NO:392), HSCOC4_PEA.sub.--1_T8 (SEQ ID NO:393),
HSCOC4_PEA.sub.--1_T11 (SEQ ID NO:394), HSCOC4_PEA.sub.--1_T12 (SEQ
ID NO:395), HSCOC4_PEA.sub.--1_T14 (SEQ ID NO:396),
HSCOC4_PEA.sub.--1_T15 (SEQ ID NO:397), HSCOC4_PEA.sub.--1_T20 (SEQ
ID NO:398), HSCOC4_PEA.sub.--1_T21 (SEQ ID NO:399),
HSCOC4_PEA.sub.--1_T25 (SEQ ID NO:400), HSCOC4_PEA.sub.--1_T28 (SEQ
ID NO:401), HSCOC4_PEA.sub.--1_T30 (SEQ ID NO:402),
HSCOC4_PEA.sub.--1_T31 (SEQ ID NO:403), HSCOC4_PEA.sub.--1_T32 (SEQ
ID NO:404) and HSCOC4_PEA.sub.--1_T40 (SEQ ID NO:405). Table 149
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00842 TABLE 149 Segment location on
transcripts Segment Segment Transcript name starting position
ending position HSCOC4_PEA.sub.--1_T1 (SEQ ID 5857 5947 NO: 387)
HSCOC4_PEA.sub.--1_T2 (SEQ ID 6002 6092 NO: 388)
HSCOC4_PEA.sub.--1_T3 (SEQ ID 6115 6205 NO: 389)
HSCOC4_PEA.sub.--1_T4 (SEQ ID 5923 6013 NO: 390)
HSCOC4_PEA.sub.--1_T5 (SEQ ID 5843 5933 NO: 391)
HSCOC4_PEA.sub.--1_T7 (SEQ ID 5541 5631 NO: 392)
HSCOC4_PEA.sub.--1_T8 (SEQ ID 7235 7325 NO: 393)
HSCOC4_PEA.sub.--1_T11 (SEQ ID 6793 6883 NO: 394)
HSCOC4_PEA.sub.--1_T12 (SEQ ID 7439 7529 NO: 395)
HSCOC4_PEA.sub.--1_T14 (SEQ ID 6137 6227 NO: 396)
HSCOC4_PEA.sub.--1_T15 (SEQ ID 5697 5787 NO: 397)
HSCOC4_PEA.sub.--1_T20 (SEQ ID 6219 6309 NO: 398)
HSCOC4_PEA.sub.--1_T21 (SEQ ID 7400 7490 NO: 399)
HSCOC4_PEA.sub.--1_T25 (SEQ ID 6149 6239 NO: 400)
HSCOC4_PEA.sub.--1_T28 (SEQ ID 6331 6421 NO: 401)
HSCOC4_PEA.sub.--1_T30 (SEQ ID 5641 5731 NO: 402)
HSCOC4_PEA.sub.--1_T31 (SEQ ID 5829 5919 NO: 403)
HSCOC4_PEA.sub.--1_T32 (SEQ ID 6273 6363 NO: 404)
HSCOC4_PEA.sub.--1_T40 (SEQ ID 4782 4872 NO: 405)
[2139] Variant protein alignment to the previously known
protein:
[2140] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2141] Sequence Documentation:
[2142] Alignment of: HSCOC4_PEA.sub.--1_P3 (SEQ ID
NO:488).times.CO4_HUMAN (SEQ ID NO:485).
[2143] Alignment segment 1/1: TABLE-US-00843 Quality: 8438.00
Escore: 0 Matching length: 870 Total length: 870 Matching Percent
Similarity: 99.66 Matching Percent 99.66 Identity: Total Percent
Similarity: 99.66 Total Percent Identity: 99.66 Gaps: 0
TABLE-US-00063 Alignment: TABLE-US-00844 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVRPHRS 870 ||||||||||||||| | | 851
LRPVLYNYLDKNLTVSVHVS 870
[2144] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2145] Sequence Documentation:
[2146] Alignment of: HSCOC4_PEA.sub.--1_P5 (SEQ ID
NO:489).times.CO4_HUMAN (SEQ ID NO:485).
[2147] Alignment segment 1/1: TABLE-US-00845 Quality: 7969.00
Escore: 0 Matching length: 818 Total length: 818 Matching Percent
Similarity: 100.00 Matching Percent 100.00 Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00064 Alignment: TABLE-US-00846 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DEVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNEEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLRLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKG 818 |||||||||||||||||| 801 DSLTTWEIHGLSLSKTKG
818
[2148] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2149] Sequence Documentation:
[2150] Alignment of: HSCOC4_PEA.sub.--1_P6 (SEQ ID
NO:490).times.CO4_HUMAN (SEQ ID NO:485).
[2151] Alignment segment 1/1: TABLE-US-00847 Quality: 10211.00
Escore: 0 Matching length: 1052 Total length: 1052 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00065 Alignment TABLE-US-00848 . . . 1
MRLLWGLIWASSFETLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVEREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 1051 KG
1052 || 1051 KG 1052
[2152] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2153] Sequence Documentation:
[2154] Alignment of: HSCOC4_PEA.sub.--1_P12 (SEQ ID
NO:491).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2155] Alignment segment 1/1: TABLE-US-00849 Quality: 13367.00
Escore: 0 Matching length: 1380 Total length: 1380 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00066 Alignment: TABLE-US-00850 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKV 1380 ||||||||||||||||||||||||||||||
1351 IRGLEEELQFSLGSKINVKVGGNSKGTLKV 1380
[2156] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2157] Sequence Documentation:
[2158] Alignment of: HSCOC4_PEA.sub.--1_P15 (SEQ ID
NO:492).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2159] Alignment segment 1/1: TABLE-US-00851 Quality: 13174.00
Escore: 0 Matching length: 1359 Total length: 1359 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00067 Alignment TABLE-US-00852 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGH0LFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLTQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQ 1359 ||||||||| 1351 IRGLEEELQ 1359
[2160] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2161] Sequence Documentation:
[2162] Alignment of: HSCOC4_PEA.sub.--1_P16 (SEQ ID
NO:493).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2163] Alignment segment 1/1: TABLE-US-00853 Quality: 14137.00
Escore: 0 Matching length: 1457 Total length: 1457 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00068 Alignment TABLE-US-00854 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTNEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPK 1457 ||||||| 1451 RRREAPK 1457
[2164] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2165] Sequence Documentation:
[2166] Alignment of: HSCOC4_PEA.sub.--1_P20 (SEQ ID
NO:494).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2167] Alignment segment 1/1: TABLE-US-00855 Quality: 12641.00
Escore: 0 Matching length: 1303 Total length: 1303 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00069 Alignment TABLE-US-00856 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKCSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSEYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVEEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 1301 STQ
1303 ||| 1301 STQ 1303
[2168] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2169] Sequence Documentation:
[2170] Alignment of: HSCOC4_PEA.sub.--1_P9 (SEQ ID
NO:495).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2171] Alignment segment 1/1: TABLE-US-00857 Quality: 14831.00
Escore: 0 Matching length: 1529 Total length: 1529 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00070 Alignment TABLE-US-00858 . . . 1
MRLLWGLTWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGRLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DRPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSV 1529 |||||||||||||||||||||||||||||
1501 LEKLTSLSDRYVSHFETEGPHVLLYFDSV 1529
[2172] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2173] Sequence Documentation:
[2174] Alignment of: HSCOC4_PEA.sub.--1_P22 (SEQ ID
NO:496).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2175] Alignment segment 1/1: TABLE-US-00859 Quality: 16066.00
Escore: 0 Matching length: 1654 Total length: 1654 Matching Percent
100.00 Matching Percent 99.94 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 99.94 Gaps: 0
TABLE-US-00071 Alignment TABLE-US-00860 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYTYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGTTDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWTASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550
|||||||||||||||||||||||||||||||||||||||||||||||||| 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550 . . . 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRR 1600
|||||||||||||||||||||||||||||||||||||||||||||||||| 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRR 1600 . . . 1601
ALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQV 1650
|||||||||||||||||||||||||||||||||||||||||||||||||| 1601
ALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQV 1650 1651 LHFS
1654 |||: 1651 LHFT 1654
[2176] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2177] Sequence Documentation:
[2178] Alignment of: HSCOC4_PEA.sub.--1_P23 (SEQ ID
NO:497).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2179] Alignment segment 1/1: TABLE-US-00861 Quality: 15806.00
Escore: 0 Matching length: 1626 Total length: 1626 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00072 Alignment TABLE-US-00862 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSEFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPRPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDTPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550
|||||||||||||||||||||||||||||||||||||||||||||||||| 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550 . . . 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRR 1600
|||||||||||||||||||||||||||||||||||||||||||||||||| 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRR 1600 . . . 1601
ALERGLQDEDGYRMKFACYYPRVEYG 1626 |||||||||||||||||||||||||| 1601
ALERGLQDEDGYRMKFACYYPRVEYG 1626
[2180] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2181] Sequence Documentation:
[2182] Alignment of: HSCOC4_PEA.sub.--1_P24 (SEQ ID
NO:498).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2183] Alignment segment 1/1: TABLE-US-00863 Quality: 14823.00
Escore: 0 Matching length: 1528 Total length: 1528 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00073 Alignment: TABLE-US-00864 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLESPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDS 1528 |||||||||||||||||||||||||||| 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDS 1528
[2184] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2185] Sequence Documentation:
[2186] Alignment of: HSCOC4_PEA.sub.--1_P25 (SEQ ID
NO:499).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2187] Alignment segment 1/1: TABLE-US-00865 Quality: 15464.00
Escore: 0 Matching length: 1593 Total length: 1593 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00074 Alignment: TABLE-US-00866 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGRLDEMQLDTQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550
|||||||||||||||||||||||||||||||||||||||||||||||||| 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550 . . . 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEG 1593
||||||||||||||||||||||||||||||||||||||||||| 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEG 1593
[2188] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2189] Sequence Documentation:
[2190] Alignment of: HSCOC4_PEA.sub.--1_P26 (SEQ ID
NO:500).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2191] Alignment segment 1/1: TABLE-US-00867 Quality: 15464.00
Escore: 0 Matching length: 1593 Total length: 1593 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total. Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00075 Alignment: TABLE-US-00868 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDCDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550
|||||||||||||||||||||||||||||||||||||||||||||||||| 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQ 1550 . . . 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEG 1593
||||||||||||||||||||||||||||||||||||||||||| 1551
PASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEG 1593
[2192] Sequence name: CO4_HUMAN_V3 (SEQ ID NO:487)
[2193] Sequence Documentation:
[2194] Alignment of: HSCOC4_PEA.sub.--1_P30 (SEQ ID
NO:501).times.CO4_HUMAN_V3 (SEQ ID NO:487).
[2195] Alignment segment 1/1: TABLE-US-00869 Quality: 11940.00
Escore: 0 Matching length: 1232 Total length: 1232 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00076 Alignment TABLE-US-00870 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGS 1232
|||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGS 1232
[2196] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2197] Sequence Documentation:
[2198] Alignment of: HSCOC4_PEA.sub.--1_P38 (SEQ ID
NO:502).times.CO4_HUMAN (SEQ (SEQ ID NO:485).
[2199] Alignment segment 1/1: TABLE-US-00871 Quality: 7969.00
Escore: 0 Matching length: 818 Total length: 818 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00077 Alignment TABLE-US-00872 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKG 818 |||||||||||||||||| 801 DSLTTWEIHGLSLSKTKG
818
[2200] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2201] Sequence Documentation:
[2202] Alignment of: HSCOC4_PEA.sub.--1_P39 (SEQ ID
NO:503).times.CO4_HUMAN (SEQ ID NO:485).
[2203] Alignment segment 1/1: TABLE-US-00873 Quality: 3766.00
Escore: 0 Matching length: 387 Total length: 387 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00078 Alingment TABLE-US-00874 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQ 387
||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQ 387
[2204] Sequence name: CO4_HUMAN (SEQ ID NO:485)
[2205] Sequence Documentation:
[2206] Alignment of: HSCOC4_PEA.sub.--1_P40 (SEQ ID
NO:504).times.CO4_HUMAN (SEQ ID NO:485).
[2207] Alignment segment 1/1: TABLE-US-00875 Quality: 2309.00
Escore: 0 Matching length: 236 Total length: 236 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00079 Alignment: TABLE-US-00876 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKY 236
|||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKY 236
[2208] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2209] Sequence Documentation:
[2210] Alignment of: HSCOC4_PEA.sub.--1_P41 (SEQ ID
NO:505).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2211] Alignment segment 1/1: TABLE-US-00877 Quality: 14831.00
Escore: 0 Matching length: 1529 Total length: 1529 Matching Percent
100.00 Matching Percent 100.00 Similarity: Identity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00080 Alignment TABLE-US-00878 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPELLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPELLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYPVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVRVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQTEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQTEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500
|||||||||||||||||||||||||||||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRAD 1500 . . . 1501
LEKLTSLSDRYVSHFETEGPHVLLYFDSV 1529 |||||||||||||||||||||||||||||
1501 LEKLTSLSDRYVSHFETEGPHVLLYFDSV 1529
[2212] Sequence name: CO4_HUMAN_V1 (SEQ ID NO:486)
[2213] Sequence Documentation:
[2214] Alignment of: HSCOC4_PEA.sub.--1_P42 (SEQ ID
NO:506).times.CO4_HUMAN_V1 (SEQ ID NO:486).
[2215] Alignment segment 1/1: TABLE-US-00879 Quality: 14480.00
Escore: 0 Matching length: 1506 Total length: 1544 Matching Percent
99.93 Matching Percent 99.87 Similarity: Identity: Total Percent
Similarity: 97.47 Total Percent Identity: 97.41 Gaps: 1
TABLE-US-00081 Alignment: TABLE-US-00880 . . . 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQ 50 . . . 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
VVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLH 100 . . . 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
QLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIY 150 . . . 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
NPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQD 200 . . . 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
DFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKP 250 . . . 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
YILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLE 300 . . . 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
SQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGG 350 . . . 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASG 400 . . . 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
IPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA 450 . . . 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGA 500 . . . 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
TFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHG 550 . . . 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
DHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVA 600 . . . 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAA 650 . . . 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700
|||||||||||||||||||||||||||||||||||||||||||||||||| 651
GLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQYASPTAK 700 . . . 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQEAESLRKKSRDKG 750
|||||||||||||||||||||||||||||||||||||||||||||||||| 701
RCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQEAESLRKKSRDKG 750 . . . 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800
|||||||||||||||||||||||||||||||||||||||||||||||||| 751
QAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLP 800 . . . 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850
|||||||||||||||||||||||||||||||||||||||||||||||||| 801
DSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLE 850 . . . 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900
|||||||||||||||||||||||||||||||||||||||||||||||||| 851
LRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFS 900 . . . 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950
|||||||||||||||||||||||||||||||||||||||||||||||||| 901
VVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNP 950 . . . 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000
|||||||||||||||||||||||||||||||||||||||||||||||||| 951
LDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGV 1000 . . . 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050
|||||||||||||||||||||||||||||||||||||||||||||||||| 1001
ASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQ 1050 . . . 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100
|||||||||||||||||||||||||||||||||||||||||||||||||| 1051
KGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKL 1100 . . . 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150
|||||||||||||||||||||||||||||||||||||||||||||||||| 1101
QETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALH 1150 . . . 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200
|||||||||||||||||||||||||||||||||||||||||||||||||| 1151
HGLAVFQDEGAEPLKQRVEASISKASSFLGEKASAGLLGAHAAAITAYAL 1200 . . . 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250
|||||||||||||||||||||||||||||||||||||||||||||||||| 1201
TLTKAPADLRGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNP 1250 . . . 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300
|||||||||||||||||||||||||||||||||||||||||||||||||| 1251
SDPMPQAPALWIETTAYALLHLLLHEGKAEMADQAAAWLTRQGSFQGGFR 1300 . . . 1301
STQDTVIALDALSAWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350
|||||||||||||||||||||||||||||||||||||||||||||||||| 1301
STQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQ 1350 . . . 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400
|||||||||||||||||||||||||||||||||||||||||||||||||| 1351
IRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIE 1400 . . . 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450
|||||||||||||||||||||||||||||||||||||||||||||||||| 1401
VTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNR 1450 . . . 1451
RRREAPKVVEEQESRVHYTVCIWWAPGAALGQGREGRTQAGAGLLEPAQA 1500
||||||||||||||||||||||| 1451
RRREAPKVVEEQESRVHYTVCIW........................... 1473 . . . 1501
EPGRQLTRLHRRNGKVGLSGMAIADVTLLSGFHALRADLEKVWS 1544
||||||||||||||||||||||||||||||: | 1474
...........RNGKVGLSGMAIADVTLLSGFHALRADLEKLTS 1506
Description for Cluster HUMTREFAC
[2216] Cluster HUMTREFAC features 2 transcript(s) and 7 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00881 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HUMTREFAC_PEA.sub.--2_T4 507
HUMTREFAC_PEA.sub.--2_T5 508.
[2217] TABLE-US-00882 TABLE 2 Segments of interest Segment Name
Sequence ID No. HUMTREFAC_PEA.sub.--2_node.sub.--0 509
HUMTREFAC_PEA.sub.--2_node.sub.--9 510
HUMTREFAC_PEA.sub.--2_node.sub.--2 511
HUMTREFAC_PEA.sub.--2_node.sub.--3 512
HUMTREFAC_PEA.sub.--2_node.sub.--4 513
HUMTREFAC_PEA.sub.--2_node.sub.--5 514
HUMTREFAC_PEA.sub.--2_node.sub.--8 515
[2218] TABLE-US-00883 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) HUMTREFAC_PEA.sub.--2_P7
517 HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO: 508)
HUMTREFAC_PEA.sub.--2_P8 518 HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:
507)
[2219] These sequences are variants of the known protein Trefoil
factor 3 precursor (SwissProt accession identifier TFF3_HUMAN;
known also according to the synonyms Intestinal trefoil factor;
hP1.B), SEQ ID NO: 516, referred to herein as the previously known
protein.
[2220] Protein Trefoil factor 3 precursor (SEQ ID NO:516) is known
or believed to have the following function(s): May have a role in
promoting cell migration (motogen). The sequence for protein
Trefoil factor 3 precursor is given at the end of the application,
as "Trefoil factor 3 precursor amino acid sequence". Known
polymorphisms for this sequence are as shown in Table 4.
TABLE-US-00884 TABLE 4 Amino acid mutations for Known Protein SNP
position(s) on amino acid sequence Comment 74-76 QEA->TRKT
[2221] Protein Trefoil factor 3 precursor (SEQ ID NO:516)
localization is believed to be Secreted.
[2222] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: defense response;
digestion, which are annotation(s) related to Biological Process;
and extracellular, which are annotation(s) related to Cellular
Component.
[2223] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nim.nih.gov/projects/LocusLink/.
[2224] Cluster HUMTREFAC can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 36 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[2225] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 36 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: a mixture of malignant tumors from
different tissues, breast malignant tumors, pancreas carcinoma and
prostate cancer. TABLE-US-00885 TABLE 5 Normal tissue distribution
Name of Tissue Number adrenal 40 colon 797 epithelial 95 general 39
liver 0 lung 57 lymph nodes 3 breast 0 muscle 3 pancreas 2 prostate
16 stomach 0 Thyroid 257 uterus 54
[2226] TABLE-US-00886 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 6.4e-01
6.9e-01 7.1e-01 1.1 7.8e-01 0.9 colon 4.6e-01 5.7e-01 9.7e-01 0.5 1
0.4 epithelial 2.4e-02 3.4e-01 9.5e-10 2.0 5.3e-02 1.1 general
2.5e-04 3.9e-02 1.4e-28 3.6 1.9e-1 0 1.9 liver 1 6.8e-01 1 1.0
6.9e-01 1.4 lung 4.8e-01 7.6e-01 2.2e-03 1.0 1.6e-01 0.5 lymph
nodes 5.1e-01 8.0e-01 2.3e-02 5.0 1.9e-01 2.1 breast 7.6e-02
1.2e-01 3.1e-06 12.0 1.1e-03 6.5 muscle 9.2e-01 4.8e-01 1 0.8
3.9e-01 2.1 pancreas 1.2e-01 2.4e-01 5.7e-03 6.5 2.1e-02 4.6
prostate 1.5e-01 2.7e-01 9.9e-10 8.1 3.1e-07 5.7 stomach 3.0e-01
1.3e-01 5.0e-01 2.0 6.7e-02 2.8 Thyroid 6.4e-01 6.4e-01 9.6e-01 0.5
9.6e-01 0.5 uterus 4.1e-01 7.3e-01 7.5e-02 1.3 4.0e-01 0.8
[2227] As noted above, cluster HUMTREFAC features 2 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Trefoil factor 3
precursor (SEQ ID NO:516). A description of each variant protein
according to the present invention is now provided.
[2228] Variant protein HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:517)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2229] Variant protein HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:51 7)
also has the following non-silent SNPs (Single Nucleotide
Polymorphisms) as listed in Table 7, (given according to their
position(s) on the amino acid sequence, with the alternative amino
acid(s) listed; the last column indicates whether the SNP is known
or not; the presence of known SNPs in variant protein
HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:517) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00887 TABLE 7 Amino acid mutations SNP
position(s) on amino acid Alternative sequence amino acid(s)
Previously known SNP? 5 A->S No 5 A->T No 14 A->V Yes 43
L->M No 60 P->S Yes 123 S->*Yes
[2230] Variant protein HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:517) is
encoded by the following transcript(s): HUMTREFAC_PEA.sub.--2_T5
(SEQ ID NO:508), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508) is shown in bold; this
coding portion starts at position 278 and ends at position 688. The
transcript also has the following SNPs as listed in Table 8 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HUMTREFAC_PEA.sub.--2_P7 (SEQ ID NO:517) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00888 TABLE 8 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 233 A->G Yes 290 G->A No 290 G->T No
318 C->T Yes 404 C->A No 404 C->T No 455 C->T Yes 645
C->A Yes 685 C->T No
[2231] Variant protein HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507). An alignment is given to
the known protein (Trefoil factor 3 precursor (SEQ ID NO:516)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2232] Comparison report between HUMTREFAC_PEA.sub.--2_P8 (SEQ ID
NO:518) and TFF3_HUMAN (SEQ ID NO:516):
[2233] 1.An isolated chimeric polypeptide encoding for
HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), comprising a first amino
acid sequence being at least 90% homologous to
MAARALCMLGLVLALLSSSSAEEYVGL corresponding to amino acids 1-27 of
TFF3_HUMAN (SEQ ID NO:516), which also corresponds to amino acids
1-27 of HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence WKVHLPKGEGFSSG (SEQ ID NO:996) corresponding to amino
acids 28-41 of HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2234] 2.An isolated polypeptide encoding for a tail of
HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
WKVHLPKGEGFSSG (SEQ ID NO:996) in HUMTREFAC_PEA.sub.--2_P8 (SEQ ID
NO:518).
[2235] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2236] Variant protein HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518)
also has the following non-silent SNPs (Single Nucleotide
Polymorphisms) as listed in Table 9, (given according to their
position(s) on the amino acid sequence, with the alternative amino
acid(s) listed; the last column indicates whether the SNP is known
or not; the presence of known SNPs in variant protein
HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518) sequence provides support
for the deduced sequence of this variant protein according to the
present invention). TABLE-US-00889 TABLE 9 Amino acid mutations SNP
position(s) on amino acid Alternative sequence amino acid(s)
Previously known SNP? 5 A->S No 5 A->T No 14 A->V Yes
[2237] Variant protein HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518) is
encoded by the following transcript(s): HUMTREFAC_PEA.sub.--2_T4
(SEQ ID NO:507), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507) is shown in bold; this
coding portion starts at position 278 and ends at position 400. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HUMTREFAC_PEA.sub.--2_P8 (SEQ ID NO:518) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00890 TABLE 10 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 233 A->G Yes 290 G->A No 290 G->T No
318 C->T Yes 515 C->A No 515 C->T No 566 C->T Yes 756
C->A Yes 796 C->T No 1265A->C No 1266A->T No
[2238] As noted above, cluster HUMTREFAC features 7 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[2239] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--0 (SEQ ID
NO:509) according to the present invention is supported by 188
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507) and
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 11 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00891 TABLE 11 Segment location on transcripts
Segment Segment ending Transcript name starting position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 1 359 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 1 359 NO: 508)
[2240] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--9 (SEQ ID
NO:510) according to the present invention is supported by 150
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507) and
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 12 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00892 TABLE 12 Segment location on transcripts
Segment Segment ending Transcript name starting position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 681 1266 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 570 747 NO: 508)
[2241] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2242] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--2 (SEQ ID
NO:511) according to the present invention is supported by 4
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507). Table 13
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00893 TABLE 13 Segment location on
transcripts Segment Segment ending Transcript name starting
position position HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 360 470 NO: 507)
[2243] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--3 (SEQ ID
NO:512) according to the present invention is supported by 10
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507) and
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 14 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00894 TABLE 14 Segment location on transcripts
Segment Segment ending Transcript name starting position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 471 514 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 360 403 NO: 508)
[2244] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--4 (SEQ ID
NO:513) according to the present invention is supported by 197
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507) and
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 15 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00895 TABLE 15 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 515 611 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 404 500 NO: 508)
[2245] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--5 (SEQ ID
NO:514) according to the present invention is supported by 187
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMTREFAC_PEA.sub.--2 T4 (SEQ ID NO:507) and
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 16 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-00896 TABLE 16 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 612 661 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 501 550 NO: 508)
[2246] Segment cluster HUMTREFAC_PEA.sub.--2_node.sub.--8 (SEQ ID
NO:515) according to the present invention can be found in the
following transcript(s): HUMTREFAC_PEA.sub.--2_T4 (SEQ ID NO:507)
and HUMTREFAC_PEA.sub.--2_T5 (SEQ ID NO:508). Table 17 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-00897 TABLE 17 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HUMTREFAC_PEA.sub.--2_T4 (SEQ ID 662 680 NO: 507)
HUMTREFAC_PEA.sub.--2_T5 (SEQ ID 551 569 NO: 508)
[2247] Variant protein alignment to the previously known
protein:
[2248] Sequence name: TFF3_HUMAN (SEQ ID NO:516)
[2249] Sequence Documentation:
[2250] Alignment of: HUMTREFAC_PEA.sub.--2_P8 (SEQ ID
NO:518).times.TFF3_HUMAN (SEQ ID NO:516).
[2251] Alignment segment 1/1: TABLE-US-00898 Quality: 246.00
Escore: 0 Matching length: 27 Total length: 27 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00082 Alignment: TABLE-US-00899 . . . 1
MAARALCMLGLVLALLSSSSAEEYVGL 27 ||||||||||||||||||||||||||| 1
MAARALCMLGLVLALLSSSSAEEYVGL 27
Description for Cluster HUMOSTRO
[2252] Cluster HUMOSTRO features 3 transcript(s) and 30 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00900 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 519
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 520
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 521
[2253] TABLE-US-00901 TABLE 2 Segments of interest Segment Name
Sequence ID No. HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--0 522
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--10 523
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--16 524
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--23 525
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--31 526 HUMOSTRO
PEA.sub.--1_PEA.sub.--1_node.sub.--43 527
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--3 528
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--5 529
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--7 530
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--8 531
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--15 532
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--17 533
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--20 534
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--21 535
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--22 536
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--24 537
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--26 538
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--27 539
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--28 540
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--29 541
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--30 542
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--32 543
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--34 544
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--36 545
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--37 546
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--38 547
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--39 548
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--40 549
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--41 550
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--42 551
[2254] TABLE-US-00902 TABLE 3 Proteins of interest Sequence
Corresponding Protein Name ID No. Transcript(s)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 553
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 554
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520)
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 555
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521)
[2255] These sequences are variants of the known protein
Osteopontin precursor (SwissProt accession identifier OSTP_HUMAN;
known also according to the synonyms Bone sialoprotein 1; Urinary
stone protein; Secreted phosphoprotein 1; SPP-1; Nephropontin;
Uropontin), SEQ ID NO: 552, referred to herein as the previously
known protein.
[2256] Protein Osteopontin precursor (SEQ ID NO:552) is known or
believed to have the following function(s): Binds tightly to
hydroxyapatite. Appears to form an integral part of the mineralized
matrix. Probably important to cell-matrix interaction; Acts as a
cytokine involved in enhancing production of interferon-gamma and
interleukin-12 and reducing production of interleukin-10 and is
essential in the pathway that leads to type I immunity (By
similarity). The sequence for protein Osteopontin precursor (SEQ ID
NO:552) is given at the end of the application, as "Osteopontin
precursor (SEQ ID NO:552) amino acid sequence". Known polymorphisms
for this sequence are as shown in Table 4. TABLE-US-00903 TABLE 4
Amino acid mutations for Known Protein SNP position(s) on amino
acid sequence Comment 301 R->H (in dbSNP: 4660).
/FTId=VAR.sub.--014717. 188 D->H 237 T->A 275-278
SHEF->GNSL
[2257] Protein Osteopontin precursor (SEQ ID NO:552) localization
is believed to be Secreted.
[2258] The previously known protein also has the following
indication(s) and/or potential therapeutic use(s): Regeneration,
bone. It has been investigated for clinical/therapeutic use in
humans, for example as a target for an antibody or small molecule,
and/or as a direct therapeutic; available information related to
these investigations is as follows. Potential pharmaceutically
related or therapeutically related activity or activities of the
previously known protein are as follows: Bone formation stimulant.
A therapeutic role for a protein represented by the cluster has
been predicted. The cluster was assigned this field because there
was information in the drug database or the public databases (e.g.,
described herein above) that this protein, or part thereof, is used
or can be used for a potential therapeutic indication:
Musculoskeletal.
[2259] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: ossification;
anti-apoptosis; inflammatory response; cell-matrix adhesion;
cell-cell signaling, which are annotation(s) related to Biological
Process; defense/immunity protein; cytokine; integrin ligand;
protein binding; growth factor; apoptosis inhibitor, which are
annotation(s) related to Molecular Function; and extracellular
matrix, which are annotation(s) related to Cellular Component.
[2260] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[2261] Cluster HUMOSTRO can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 37 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[2262] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 37 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues, lung malignant tumors,
breast malignant tumors, ovarian carcinoma and skin malignancies.
TABLE-US-00904 TABLE 5 Normal tissue distribution Name of Tissue
Number adrenal 4 bladder 0 bone 897 brain 506 colon 69 epithelial
548 general 484 head and neck 50 kidney 5618 liver 4 lung 10 lymph
nodes 75 breast 8 bone marrow 62 muscle 37 ovary 40 pancreas 845
prostate 48 skin 13 stomach 73 Thyroid 0 uterus 168
[2263] TABLE-US-00905 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 1.5e-01
2.1e-01 2.0e-02 4.6 4.4e-02 3.6 bladder 1.2e-01 9.2e-02 5.7e-02 4.1
2.1e-02 4.3 bone 4.9e-01 7.4e-01 4.1e-06 0.6 5.4e-01 0.4 brain
6.6e-01 7.0e-01 3.2e-01 0.6 1 0.4 colon 2.7e-01 4.0e-01 3.1e-01 1.5
5.2e-01 1.1 epithelial 2.0e-07 1.6e-03 9.8e-01 0.7 1 0.5 general
1.2e-06 1.2e-02 7.9e-01 0.8 1 0.6 head and neck 3.4e-01 5.0e-01 1
0.7 1 0.7 kidney 6.8e-01 7.4e-01 1 0.2 1 0.1 liver 3.3e-01 2.5e-01
1 1.8 2.3e-01 2.6 lung 4.3e-04 4.6e-03 2.1e-30 15.0 2.8e-27 23.5
lymph nodes 6.7e-01 8.7e-01 8.1e-01 0.7 9.9e-01 0.3 breast 2.3e-01
3.0e-01 1.9e-04 6.2 4.1e-03 4.3 bone marrow 7.5e-01 7.8e-01 1 0.3
2.0e-02 1.2 muscle 4.0e-02 7.5e-02 1.1e-01 4.6 5.1e-01 1.5 ovary
4.7e-02 8.4e-02 1.9e-05 5.4 8.3e-04 3.7 pancreas 5.0e-02 3.3e-01 1
0.3 1 0.2 prostate 8.5e-01 9.0e-01 8.9e-01 0.7 9.5e-01 0.6 skin
1.6e-01 1.6e-01 1.2e-10 12.6 5.2e-04 4.1 stomach 1.5e-01 6.3e-01
5.0e-01 1.2 9.4e-01 0.6 Thyroid 2.9e-01 2.9e-01 5.9e-02 2.0 5.9e-02
2.0 uterus 6.1e-02 5.7e-01 1.1e-01 1.3 7.0e-01 0.7
[2264] As noted above, cluster HUMOSTRO features 3 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Osteopontin precursor
(SEQ ID NO:552). A description of each variant protein according to
the present invention is now provided.
[2265] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID
NO:553) according to the present invention has an amino acid
sequence as given at the end of the application; it is encoded by
transcript(s) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519).
An alignment is given to the known protein (Osteopontin precursor
(SEQ ID NO:552)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2266] Comparison report between
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553) and OSTP_HUMAN
(SEQ ID NO:552):
[2267] 1.An isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQ
corresponding to amino acids 1-58 of OSTP_HUMAN (SEQ ID NO:552),
which also corresponds to amino acids 1-58 of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), and a second
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VFLNFS (SEQ ID NO:997) corresponding to amino acids 59-64
of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2268] 2.An isolated polypeptide encoding for a tail of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
VFLNFS (SEQ ID NO:997) in HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ
ID NO:553).
[2269] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because of manual inspection of known
protein localization and/or gene structure.
[2270] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID
NO:553) also has the following non-silent SNPs (Single Nucleotide
Polymorphisms) as listed in Table 7, (given according to their
position(s) on the amino acid sequence, with the alternative amino
acid(s) listed; the last column indicates whether the SNP is known
or not; the presence of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00906 TABLE 7 Amino
acid mutations SNP position(s) on Alternative Previously amino acid
sequence amino acid(s) known SNP? 7 C->W No 31 Q->R No 47
D->V Yes 49 S->P No
[2271] The glycosylation sites of variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553), as compared
to the known protein Osteopontin precursor (SEQ ID NO:552), are
described in Table 8 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00907 TABLE 8
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 79 no 106
no
[2272] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID
NO:553) is encoded by the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID
NO:519) is shown in bold; this coding portion starts at position
199 and ends at position 390. The transcript also has the following
SNPs as listed in Table 9 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID NO:553) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00908 TABLE 9 Nucleic
acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 136 A->G Yes 154 T->No 159
G->T Yes 219 C->G No 274->G No 290 A->G No 338 A->T
Yes 343 T->C No 413 G->C Yes 707 C->T Yes 708 C->A Yes
715 A->G Yes 730 A->C No 730 A->G No 746 T->C Yes 767
C->T No 779 G->A Yes 866->G No 869 T->No 889->A No
891 A->C No 891 A->G No 905 T->C No 910->G No 910->T
No 997A->G No 1026 G->C No 1042->G No 1042->T No 1071
A->No 1071 A->C No 1098 A->No 1105 C->T No 1124->G
No 1135 G->A Yes 1136 T->No 1136 T->G No 1173A->C No
1173A->G No 1179A->G No 1214 C->T Yes 1246T->No
1246T->A No 1359A->No 1359A->G No 1362 T->No 1365
C->T Yes 1366 G->A Yes 1408 A->C No 1418 A->C No 1433
A->C No 1456A->C No 1524T->A No 1524T->C No 1547A->G
Yes 1553T->No 1574->G No 1654 A->C Yes 1691 A->G No
1703 A->C Yes 1755A->C No 1764T->No
[2273] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID
NO:554) according to the present invention has an amino acid
sequence as given at the end of the application; it is encoded by
transcript(s) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520).
An alignment is given to the known protein (Osteopontin precursor
(SEQ ID NO:552)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2274] Comparison report between HUMOSTRO
PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554) and OSTP_HUMAN (SEQ ID
NO:552):
[2275] 1.An isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31
of OSTP_HUMAN (SEQ ID NO:552), which also corresponds to amino
acids 1-31 of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554),
and a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence H corresponding to amino acids 32-32 of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2276] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2277] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID
NO:554) also has the following non-silent SNPs (Single Nucleotide
Polymorphisms) as listed in Table 10, (given according to their
position(s) on the amino acid sequence, with the alternative amino
acid(s) listed; the last column indicates whether the SNP is known
or not; the presence of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00909 TABLE 10 Amino
acid mutations SNP position(s) on amino acid Alternative sequence
amino acid(s) Previously known SNP? 7 C->W No 31 Q->R No
[2278] The glycosylation sites of variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554), as compared
to the known protein Osteopontin precursor (SEQ ID NO:552), are
described in Table 11 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00910 TABLE 11
Glycosylation site(s) Position(s) on known Present in Position
amino acid sequence variant protein? in variant protein? 79 no 106
no
[2279] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID
NO:554) is encoded by the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID
NO:520) is shown in bold; this coding portion starts at position
199 and ends at position 294. The transcript also has the following
SNPs as listed in Table 12 (given according to their position on
the nucleotide sequence, with the alternative nucleic acid listed;
the last column indicates whether the SNP is known or not; the
presence of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID NO:554) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00911 TABLE 12
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 136 A->G Yes 154 T->No 159
G->T Yes 219 C->G No 274->G No 290A->G No 419 C->T
Yes 454 G->C Yes 527 A->T Yes 532 T->C No 630 C->T Yes
631 C->A Yes 638 A->G Yes 653 A->C No 653 A->G No 669
T->C Yes 690 C->T No 702 G->A Yes 789->G No 792
T->No 812->A No 814 A->C No 814 A->G No 828 T->C No
833->G No 833->T No 920 A->G No 949 G->C No 965->G
No 965->T No 994 A->No 994 A->C No 1021 A->No 1028
C->T No 1047->G No 1058 G->A Yes 1059 T->No 1059
T->G No 1096 A->C No 1096 A->G No 1102 A->G No 1137
C->T Yes 1169 T->No 1169 T->A No 1282 A->No 1282A->G
No 1285T->No 1288C->T Yes 1289G->A Yes 1331 A->C No
1341 A->C No 1356 A->C No 1379 A->C No 1447 T->A No
1447 T->C No 1470 A->G Yes 1476 T->No 1497->G No
1577A->C Yes 1614A->G No 1626A->C Yes 1678A->C No 1687
T->No
[2280] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID
NO:555) according to the present invention has an amino acid
sequence as given at the end of the application; it is encoded by
transcript(s) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521).
An alignment is given to the known protein (Osteopontin precursor
(SEQ ID NO:552)) at the end of the application. One or more
alignments to one or more previously published protein sequences
are given at the end of the application. A brief description of the
relationship of the variant protein according to the present
invention to each such aligned protein is as follows:
[2281] Comparison report between
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555) and OSTP_HUMAN
(SEQ ID NO:552):
[2282] 1. An isolated chimeric polypeptide encoding for
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555), comprising a
first amino acid sequence being at least 90% homologous to
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ corresponding to amino acids 1-31
of OSTP_HUMAN (SEQ ID NO:552), which also corresponds to amino
acids 1-31 of HYMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555),
and a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence VSIFYVFI (SEQ ID NO:998) corresponding to amino acids
32-39 of HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[2283] 2.An isolated polypeptide encoding for a tail of
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
VSIFYVFI (SEQ ID NO:998) in HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30
(SEQ ID NO:555).
[2284] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2285] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID
NO:555) also has the following non-silent SNPs (Single Nucleotide
Polymorphisms) as listed in Table 13, (given according to their
position(s) on the amino acid sequence, with the alternative amino
acid(s) listed; the last column indicates whether the SNP is known
or not; the presence of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00912 TABLE 13 Amino
acid mutations SNP position(s) on amino acid Alternative sequence
amino acid(s) Previously known SNP? 7 C->W No 31 Q->R No
[2286] The glycosylation sites of variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555), as compared
to the known protein Osteopontin precursor (SEQ ID NO:552), are
described in Table 14 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-00913 TABLE 14
Glycosylation site(s) Position(s) on known amino acid sequence
Present in variant protein? 79 no 106 no
[2287] Variant protein HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID
NO:555) is encoded by the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID
NO:521) is shown in bold; this coding portion starts at position
199 and ends at position 315. The transcript also has the following
SNPs as listed in Table 15 (given according to their position on
the nucleotide sequence, with the alternative nucleic acid listed;
the last column indicates whether the SNP is known or not; the
presence of known SNPs in variant protein
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID NO:555) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00914 TABLE 15
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 136A->G Yes 154T->No 159
G->T Yes 219 C->G No 274->G No290A->G No
[2288] As noted above, cluster HUMOSTRO features 30 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[2289] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--0 (SEQ ID NO:522)
according to the present invention is supported by 333 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519),
HUMOSTRO_PEA.sub.--1_PEA.sub.--1.sub.T16 (SEQ ID NO:520) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521). Table 16
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00915 TABLE 16 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1 184 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1 184 (SEQ ID
NO:520) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 1 184 (SEQ ID
NO:521)
[2290] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--10 (SEQ ID NO:523)
according to the present invention is supported by 4 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 17
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00916 TABLE 17 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 292 480 (SEQ
ID NO: 520)
[2291] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--16 (SEQ ID NO:524)
according to the present invention is supported by 6 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519). Table 18
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00917 TABLE 18 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 373 638 (SEQ
ID NO:519)
[2292] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--23 (SEQ ID NO:525)
according to the present invention is supported by 334 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 19
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00918 TABLE 19 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 804 967 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 727 890 (SEQ ID
NO:520)
[2293] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--31 (SEQ ID NO:526)
according to the present invention is supported by 350 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 20
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00919 TABLE 20 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1164 1393
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1087 1316 (SEQ
ID NO:520)
[2294] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--43 (SEQ ID NO:527)
according to the present invention is supported by 192 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 21
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00920 TABLE 21 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1810 1846
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1733 1769 (SEQ
ID NO:520)
[2295] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2296] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--3 (SEQ ID NO:528)
according to the present invention is supported by 353 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14(SEQ ID NO:519),
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521). Table 22
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00921 TABLE 22 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 185 210 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 185 210 (SEQ ID
NO:520) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 185 210 (SEQ ID
NO:521)
[2297] Segment cluster HUMOSTRO_.sub.--1_PEA.sub.--1_node.sub.--5
(SEQ ID NO:529) according to the present invention is supported by
353 libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID
NO:519), HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521). Table 23
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00922 TABLE 23 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 211 252 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 211 252 (SEQ ID
NO:520) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 211 252 (SEQ ID
NO:521)
[2298] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--7 (SEQ ID NO:530)
according to the present invention is supported by 357 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519),
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521). Table 24
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00923 TABLE 24 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 253 291 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 253 291 (SEQ ID
NO:520) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 253 291 (SEQ ID
NO:521)
[2299] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--8 (SEQ ID NO:531)
according to the present invention is supported by 1 libraries. The
number of libraries was determined as previously described. This
segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 (SEQ ID NO:521). Table 25
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00924 TABLE 25 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T30 292 378 (SEQ
ID NO:521)
[2300] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--15 (SEQ ID NO:532)
according to the present invention is supported by 366 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 26
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00925 TABLE 26 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 292 372 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 481 561 (SEQ ID
NO:520)
[2301] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--17 (SEQ ID NO:533)
according to the present invention is supported by 261 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 27
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00926 TABLE 27 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 639 680 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 562 603 (SEQ ID
NO:520)
[2302] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--20 (SEQ ID NO:534)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 28
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00927 TABLE 28 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 681 688 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 604 611 (SEQ ID
NO:520)
[2303] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--21 (SEQ ID NO:535)
according to the present invention is supported by 315 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 29
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00928 TABLE 29 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 689 738 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 612 661 (SEQ ID
NO:520)
[2304] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--22 (SEQ ID NO:536)
according to the present invention is supported by 322 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 30
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00929 TABLE 30 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 739 803 (SEQ
ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 662 726 (SEQ ID
NO:520)
[2305] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--24 (SEQ ID NO:537)
according to the present invention is supported by 270 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 31
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00930 TABLE 31 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 968 1004
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 891 927 (SEQ
ID NO:520)
[2306] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--26 (SEQ ID NO:538)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 32
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00931 TABLE 32 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1005 1022
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 928 945 (SEQ
ID NO:520)
[2307] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--27 (SEQ ID NO:539)
according to the present invention is supported by 260 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 33
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00932 TABLE 33 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1023 1048
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 946 971 (SEQ
ID NO:520)
[2308] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--28 (SEQ ID NO:540)
according to the present invention is supported by 273 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 34
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00933 TABLE 34 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1049 1100
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 972 1023 (SEQ
ID NO:520)
[2309] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--29 (SEQ ID NO:541)
according to the present invention is supported by 272 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 35
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00934 TABLE 35 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1101 1151
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1024 1074 (SEQ
ID NO:520)
[2310] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--30 (SEQ ID NO:542)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 36
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00935 TABLE 36 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1152 1163
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1075 1086 (SEQ
ID NO:520)
[2311] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--32 (SEQ ID NO:543)
according to the present invention is supported by 293 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 37
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00936 TABLE 37 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1394 1427
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1317 1350 (SEQ
ID NO:520)
[2312] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--34 (SEQ ID NO:544)
according to the present invention is supported by 301 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 38
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00937 TABLE 38 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1428 1468
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1351 1391 (SEQ
ID NO:520)
[2313] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--36 (SEQ ID NO:545)
according to the present invention is supported by 292 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 39
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00938 TABLE 39 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1469 1504
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1392 1427 (SEQ
ID NO:520)
[2314] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--37 (SEQ ID NO:546)
according to the present invention is supported by 295 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 40
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00939 TABLE 40 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1505 1623
(SEQ ID NO 519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1428 1546 (SEQ
ID NO:520)
[2315] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--38 (SEQ ID NO:547)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 41
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00940 TABLE 41 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1624 1634
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1547 1557 (SEQ
ID NO:520)
[2316] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--39 (SEQ ID NO:548)
according to the present invention is supported by 268 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 42
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00941 TABLE 42 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1635 1725
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1558 1648 (SEQ
ID NO:520)
[2317] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--40 (SEQ ID NO:549)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 43
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00942 TABLE 43 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1726 1743
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1649 1666 (SEQ
ID NO:520)
[2318] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--41 (SEQ ID NO:550)
according to the present invention can be found in the following
transcript(s): HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519)
and HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 44
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00943 TABLE 44 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1744 1749
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1667 1672 (SEQ
ID NO:520)
[2319] Segment cluster
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_node.sub.--42 (SEQ ID NO:551)
according to the present invention is supported by 224 libraries.
The number of libraries was determined as previously described.
This segment can be found in the following transcript(s):
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 (SEQ ID NO:519) and
HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 (SEQ ID NO:520). Table 45
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00944 TABLE 45 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T14 1750 1809
(SEQ ID NO:519) HUMOSTRO_PEA.sub.--1_PEA.sub.--1_T16 1673 1732 (SEQ
ID NO:520)
[2320] Variant protein alignment to the previously known
protein:
[2321] Sequence name: OSTP_HUMAN (SEQ ID NO:552)
[2322] Sequence Documentation:
[2323] Alignment of: HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P21 (SEQ ID
NO:553).times.OSTP HUMAN (SEQ ID NO:552).
[2324] Alignment segment 1/1: TABLE-US-00945 Quality: 578.00
Escore: 0 Matching length: 58 Total length: 58 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00083 Alignment: TABLE-US-00946 1
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYN KYPDAVATWLNPDPSQ 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYN KYPDAVATWLNPDPSQ 50 51 KQNLLAPQ
58 |||||||| 51 KQNLLAPQ 58
[2325] Sequence name: OSTP_HUMAN (SEQ ID NO:552)
[2326] Sequence Documentation:
[2327] Alignment of: HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P25 (SEQ ID
NO:554).times.OSTP_HUMAN (SEQ ID NO:552).
[2328] Alignment segment 1/1: TABLE-US-00947 Quality: 301.00
Escore: 0 Matching length: 31 Total length: 31 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00084 Alignment: TABLE-US-00948 1
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ 31 |||||||||||||||||||||||||||||||
1 MRIAVICFCLLGITCAIPVKQADSGSSEEKQ 31
[2329] Sequence name: OSTP_HUMAN (SEQ ID NO:552)
[2330] Sequence Documentation:
[2331] Alignment of: HUMOSTRO_PEA.sub.--1_PEA.sub.--1_P30 (SEQ ID
NO:555).times.OSTP_HUMAN (SEQ ID NO:552).
[2332] Alignment segment 1/1: TABLE-US-00949 Quality: 301.00
Escore: 0 Matching length: 31 Total length: 31 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00085 Alignment: TABLE-US-00950 1
MRIAVICFCLLGITCAIPVKQADSGSSEEKQ 31 |||||||||||||||||||||||||||||||
1 MRIAVICFCLLGITCAIPVKQADSGSSEEKQ 31
Description for Cluster R11723
[2333] Cluster R11723 features 6 transcript(s) and 26 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-00951 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. R11723_PEA.sub.--1_T15 556 R11723_PEA.sub.--1_T17
557 R11723_PEA.sub.--1_T19 558 R11723_PEA.sub.--1_T20 559
R11723_PEA.sub.--1_T5 560 R11723_PEA.sub.--1_T6 561
[2334] TABLE-US-00952 TABLE 2 Segments of interest Segment Name
Sequence ID No. R11723_PEA.sub.--1_node.sub.--13 562
R11723_PEA.sub.--1_node.sub.--16 563
R11723_PEA.sub.--1_node.sub.--19 564
R11723_PEA.sub.--1_node.sub.--2 565
R11723_PEA.sub.--1_node.sub.--22 566
R11723_PEA.sub.--1_node.sub.--31 567
R11723_PEA.sub.--1_node.sub.--10 568
R11723_PEA.sub.--1_node.sub.--11 569
R11723_PEA.sub.--1_node.sub.--15 570
R11723_PEA.sub.--1_node.sub.--18 571
R11723_PEA.sub.--1_node.sub.--20 572
R11723_PEA.sub.--1_node.sub.--21 573
R11723_PEA.sub.--1_node.sub.--23 574
R11723_PEA.sub.--1_node.sub.--24 575
R11723_PEA.sub.--1_node.sub.--25 576
R11723_PEA.sub.--1_node.sub.--26 577
R11723_PEA.sub.--1_node.sub.--27 578
R11723_PEA.sub.--1_node.sub.--28 579
R11723_PEA.sub.--1_node.sub.--29 580
R11723_PEA.sub.--1_node.sub.--3 581
R11723_PEA.sub.--1_node.sub.--30 582
R11723_PEA.sub.--1_node.sub.--4 583 R11723_PEA.sub.--1_node.sub.--5
584 R11723_PEA.sub.--1_node.sub.--6 585
R11723_PEA.sub.--1_node.sub.--7 586 R11723_PEA.sub.--1_node.sub.--8
587
[2335] TABLE-US-00953 TABLE 3 Proteins of interest Protein Name
Sequence ID No. R11723_PEA.sub.--1_P2 588 R11723_PEA.sub.--1_P6 589
R11723_PEA.sub.--1_P7 590 R11723_PEA.sub.--1_P13 591
R11723_PEA.sub.--1_P10 592
[2336] Cluster R11723 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the right hand column of the table and the numbers on the y-axis of
FIG. 38 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[2337] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 38 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues and kidney malignant
tumors. TABLE-US-00954 TABLE 4 Normal tissue distribution Name of
Tissue Number adrenal 0 brain 30 epithelial 3 general 17 head and
neck 0 kidney 0 lung 0 breast 0 ovary 0 pancreas 10 skin 0 uterus
0
[2338] TABLE-US-00955 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 4.2e-01
4.6e-01 4.6e-01 2.2 5.3e-01 1.9 brain 2.2e-01 2.0e-01 1.2e-02 2.8
5.0e-02 2.0 epithelial 3.0e-05 6.3e-05 1.8e-05 6.3 3.4e-06 6.4
general 7.2e-03 4.0e-02 1.3e-04 2.1 1.1e-03 1.7 head and neck 1
5.0e-01 1 1.0 7.5e-01 1.3 kidney 1.5e-01 2.4e-01 4.4e-03 5.4
2.8e-02 3.6 lung 1.2e-01 1.6e-01 1 1.6 1 1.3 breast 5.9e-01 4.4e-01
1 1.1 6.8e-01 1.5 ovary 1.6e-02 1.3e-02 1.0e-01 3.8 7.0e-02 3.5
pancreas 5.5e-01 2.0e-01 3.9e-01 1.9 1.4e-01 2.7 skin 1 4.4e-01 1
1.0 1.9e-02 2.1 uterus 1.5e-02 5.4e-02 1.9e-01 3.1 1.4e-01 2.5
[2339] As noted above, cluster R11723 features 6 transcript(s),
which were listed in Table 1 above. A description of each variant
protein according to the present invention is now provided.
[2340] Variant protein R11723_PEA.sub.--1_P2 (SEQ ID NO:588)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). The location of the variant
protein was determined according to results from a number of
different software programs and analyses, including analyses from
SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2341] Variant protein R11723_PEA.sub.--1_P2 (SEQ ID NO:588) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 6, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R11723_PEA.sub.--1_P2 (SEQ ID
NO:588) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00956
TABLE 6 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 107H->P
Yes 70 G->No 70 G->C No
[2342] Variant protein R11723_PEA.sub.--1_P2 (SEQ ID NO:588) is
encoded by the following transcript(s): R11723_PEA.sub.--1_T6 (SEQ
ID NO:561), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R11723_PEA.sub.--1_T6 (SEQ ID NO:561) is shown in bold; this coding
portion starts at position 1716 and ends at position 2051. The
transcript also has the following SNPs as listed in Table 7 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R11723_PEA.sub.--1_P2 (SEQ ID NO:588) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00957 TABLE 7 Nucleic acid SNPs
SNP position on Alternative Previously nucleotide sequence nucleic
acid known SNP? 1231 C->T Yes 1278 G->C Yes 1923 G->No
1923 G->T No 2035 A->C Yes 2048 A->C No 2057 A->G
Yes
[2343] Variant protein R11723_PEA.sub.--1_P6 (SEQ ID NO:589)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R11723_PEA.sub.--1_T15 (SEQ ID NO:556). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2344] Comparison report between R11723_PEA.sub.--1_P6 (SEQ ID
NO:589) and Q81XMO (SEQ ID NO:885):
[2345] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV
MEJQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR (SEQ ID
NO:1022) corresponding to amino acids 1-110 of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), and a second amino acid
sequence being at least 90% homologous to
MYAQALLVVGVLQRQAAAQHLHEHPPKLLRGHRVQERVDDRAEVEKRLREGEEDHV
RPEVGPRPVVLGFGRSHDPPNLVGHPAYGQCHNNQPWADTSRRERQRKEKHSMRTQ
corresponding to amino acids 1-112 of Q8IXM0, which also
corresponds to amino acids 111-222 of R11723_PEA.sub.--1_P6 (SEQ ID
NO:589), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[2346] 2. An isolated polypeptide encoding for a head of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00958
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV (SEQ ID
NO: 1022) MEQSAGIMYRKSCASSAACLIASAGSPCRGLAPGREEQRALHKAGAVGGGVR of
R11723_PEA.sub.--1_P6. (SEQ ID NO:589)
[2347] Comparison report between R11723_PEA.sub.--1_P6 (SEQ ID
NO:589) and Q96AC2 (SEQ ID NO:886):
[2348] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV
MEQSAGIMYRKSCASSMCLIASAG corresponding to amino acids 1-83 of
Q96AC2 (SEQ ID NO: 886), which also corresponds to amino acids 1-83
of R11723_PEA.sub.--1_P6 (SEQ ID NO:589), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLVVGVLQRQAAAQHLHEHPPKLL
RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO: 1023) corresponding to amino
acids 84-222 of R11723_PEA.sub.--1_P6 (SEQ ID NO:589), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[2349] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00959
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLWGVLQRQAAAQHLHEHPPKLL (SEQ ID
NO:1023) RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ in R11723_PEA.sub.--1_P6. (SEQ ID
NO:589)
[2350] Comparison report between R11723_PEA.sub.--1_P6 (SEQ ID
NO:589) and Q8N2G4 (SEQ ID NO:887):
[2351] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV
MEQSAGIMYRKSCASSMCLIASAG corresponding to amino acids 1-83 of
Q8N2G4, which also corresponds to amino acids 1-83 of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLWGVLQRQAAAQHLHEHPPKLL
RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO:1023) corresponding to amino
acids 84-222 of R11723_PEA.sub.--1_P6 (SEQ ID NO:589), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[2352] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00960
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLWGVLQRQAAAQHLHEHPPKLL (SEQ ID NO:
1023) RGHRVQERVDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ in R11723_PEA.sub.--1_P6. (SEQ ID
NO:589)
[2353] Comparison report between R11723_PEA.sub.--1_P6 (SEQ ID
NO:589) and BAC85518 (SEQ ID NO:888):
[2354] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV
MEQSAGIMYRKSCASSMCLIASAG corresponding to amino acids 24-106 of
BAC85518, which also corresponds to amino acids 1-83 of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLWGVLQRQAAAQHLHEHPPKLL
RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ (SEQ ID NO:1023) corresponding to amino
acids 84-222 of R11723_PEA.sub.--1_P6 (SEQ ID NO:589), wherein said
first and second amino acid sequences are contiguous and in a
sequential order.
[2355] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P6 (SEQ ID NO:589), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-00961
SPCRGLAPGREEQRALHKAGAVGGGVRMYAQALLWGVLQRQAAAQHLHEHPPKLL (SEQ ID
NO:1023) RGHRVQERVDDRAEVEKRLREGEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQ
CHNNQPWADTSRRERQRKEKHSMRTQ in R11723_PEA.sub.--1_P6. (SEQ ID
NO:589)
[2356] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2357] Variant protein R11723_PEA.sub.--1_P6 (SEQ ID NO:589) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R11723_PEA.sub.--1_P6 (SEQ ID
NO:589) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00962
TABLE 8 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 180
G->No 180 G->C No 217H->P Yes
[2358] Variant protein R11723_PEA.sub.--1_P6 (SEQ ID NO:589) is
encoded by the following transcript(s): R11723_PEA.sub.--1_T15 (SEQ
ID NO:556), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R11723_PEA.sub.--1_T15 (SEQ ID NO:556) is shown in bold; this
coding portion starts at position 434 and ends at position 1099.
The transcript also has the following SNPs as listed in Table 9
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein R11723_PEA.sub.--1_P6 (SEQ ID NO:589) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-00963 TABLE 9 Nucleic
acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 971 G->No 971 G->T No
1083A->C Yes 1096A->C No 1105A->G Yes
[2359] Variant protein R11723_PEA.sub.--1_P7 (SEQ ID NO:590)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R11723_PEA.sub.--1_T17 (SEQ ID NO:557). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2360] Comparison report between R11723_PEA.sub.--1_P7 (SEQ ID
NO:590) and Q96AC2 (SEQ ID NO: 886):
[2361] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIMTFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG
corresponding to amino acids 1-64 of Q96AC2 (SEQ ID NO: 886), which
also corresponds to amino acids 1-64 of R11723_PEA.sub.--1_P7 (SEQ
ID NO:590), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ
ID NO:1024) corresponding to amino acids 65-93 of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[2362] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO:1024) in
R11723_PEA.sub.--1_P7 (SEQ ID NO:590).
[2363] Comparison report between R11723_PEA.sub.--1_P7 (SEQ ID
NO:590) and Q8N2G4:
[2364] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG
corresponding to amino acids 1-64 of Q8N2G4, which also corresponds
to amino acids 1-64 of R11723_PEA.sub.--1_P7 (SEQ ID NO:590), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO:1024)
corresponding to amino acids 65-93 of R11723_PEA.sub.--1_P7 (SEQ ID
NO:590), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[2365] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO:1024) in
R11723_PEA.sub.--1_P7 (SEQ ID NO:590).
[2366] Comparison report between R11723_PEA.sub.--1_P7 (SEQ ID
NO:590) and BAC85273:
[2367] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MWVLG (SEQ ID NO:1025) corresponding to amino acids 1-5 of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), second amino acid sequence
being at least 90% homologous to
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSAG
corresponding to amino acids 22-80 of BAC85273, which also
corresponds to amino acids 6-64 of R11723_PEA.sub.--1_P7 (SEQ ID
NO:590), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ
ID NO:1024) corresponding to amino acids 65-93 of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), wherein said first, second
and third amino acid sequences are contiguous and in a sequential
order.
[2368] 2. An isolated polypeptide encoding for a head of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence MWVLG (SEQ
ID NO:1025) of R11723_PEA.sub.--1_P7 (SEQ ID NO:590).
[2369] 3. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO:1024) in
R11723_PEA.sub.--1_P7 (SEQ ID NO:590).
[2370] Comparison report between R11723_PEA.sub.--1_P7 (SEQ ID
NO:590) and BAC85518:
[2371] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSAG
corresponding to amino acids 24-87 of BAC85518, which also
corresponds to amino acids 1-64 of R11723_PEA.sub.--1_P7 (SEQ ID
NO:590), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ
ID NO:1024) corresponding to amino acids 65-93 of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[2372] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P7 (SEQ ID NO:590), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
SHCVTRLECSGTISAHCNLCLPGSNDHPT (SEQ ID NO:1024) in
R11723_PEA.sub.--1_P7 (SEQ ID NO:590).
[2373] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2374] Variant protein R11723_PEA.sub.--1_P7 (SEQ ID NO:590) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 10, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R11723_PEA.sub.--1_P7 (SEQ ID
NO:590) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00964
TABLE 10 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 67 C->S
Yes
[2375] Variant protein R11723_PEA.sub.--1_P7 (SEQ ID NO:590) is
encoded by the following transcript(s): R11723_PEA.sub.--1_T17 (SEQ
ID NO:557), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R11723_PEA.sub.--1_T17 (SEQ ID NO:557) is shown in bold; this
coding portion starts at position 434 and ends at position 712. The
transcript also has the following SNPs as listed in Table 11 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R11723_PEA.sub.--1_P7 (SEQ ID NO:590) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00965 TABLE 11 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 625 G->T Yes 633 G->C Yes 1303
C->T Yes
[2376] Variant protein R11723_PEA.sub.--1_P13 (SEQ ID NO:591)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R11723_PEA.sub.--1_T19 (SEQ ID NO:558). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2377] Comparison report between R11723_PEA.sub.--1_P13 (SEQ ID
NO:591) and Q96AC2 (SEQ ID NO: 886):
[2378] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P13 (SEQ ID NO:591) comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA
corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 886), which
also corresponds to amino acids 1-63 of R11723_PEA.sub.--1_P13 (SEQ
ID NO:591), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DTKRTNTLLFEMRHFAKQLTT (SEQ ID
NO:1026) corresponding to amino acids 64-84 of
R11723_PEA.sub.--1_P13 (SEQ ID NO:591), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[2379] 2. An isolated polypeptide encoding for a tail of
R1723_PEA.sub.--1_P13 (SEQ ID NO:591), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DTKRTNTLLFEMRHFAKQLTT (SEQ ID NO:1026) in R11723_PEA.sub.--1_P13
(SEQ ID NO:591).
[2380] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2381] Variant protein R11723_PEA.sub.--1_P13 (SEQ ID NO:591) is
encoded by the following transcript(s): R11723_PEA.sub.--1_T19 (SEQ
ID NO:558) and R11723_PEA.sub.--1_T5 (SEQ ID NO:560), for which the
sequence(s) is/are given at the end of the application. The coding
portion of transcript R11723_PEA.sub.--1_T19 (SEQ ID NO:558) is
shown in bold; this coding portion starts at position 434 and ends
at position 685. The transcript also has the following SNPs as
listed in Table 12 (given according to their position on the
nucleotide sequence, with the alternative nucleic acid listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R1172_PEA.sub.--1_P13 (SEQ ID
NO:591) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00966
TABLE 12 Nucleic acid SNPs SNP position on Alternative Previously
nucleotide sequence nucleic acid known SNP? 778 G->T Yes 786
G->C Yes 1456 C->T Yes
[2382] Variant protein R11723_PEA.sub.--1_P10 (SEQ ID NO:592)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
R11723_PEA.sub.--1_T20 (SEQ ID NO:559). One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2383] Comparison report between R11723_PEA.sub.--1_P10 (SEQ ID
NO:592) and Q96AC2 (SEQ ID NO: 886):
[2384] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P10 (SEQ ID NO:592) comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA
corresponding to amino acids 1-63 of Q96AC2 (SEQ ID NO: 886), which
also corresponds to amino acids 1-63 of R11723_PEA.sub.--1_P10 (SEQ
ID NO:592), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID
NO: 1027) corresponding to amino acids 64-90 of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[2385] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO:1027) in
R11723_PEA.sub.--1_P10 (SEQ ID NO:592).
[2386] Comparison report between R11723_PEA.sub.--1_P10 (SEQ ID
NO:592) and Q8N2G4:
[2387] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIMTFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA
corresponding to amino acids 1-63 of Q8N2G4, which also corresponds
to amino acids 1-63 of R11723_PEA.sub.--1_P10 (SEQ ID NO:592), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO:1027)
corresponding to amino acids 64-90 of R11723_PEA.sub.--1_P10 (SEQ
ID NO:592), wherein said first and second amino acid sequences are
contiguous and in a sequential order.
[2388] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO:1027) in
R11723_PEA.sub.--1_P10 (SEQ ID NO:592).
[2389] Comparison report between R11723_PEA.sub.--1_P10 (SEQ ID
NO:592) and BAC85273:
[2390] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P10 (SEQ ID NO:592) comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MWVLG (SEQ ID NO:1025) corresponding to amino acids 1-5 of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), second amino acid sequence
being at least 90% homologous to
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSA
corresponding to amino acids 22-79 of BAC85273, which also
corresponds to amino acids 6-63 of R11723_PEA.sub.--1_P10 (SEQ ID
NO:592), and a third amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID
NO:1027) corresponding to amino acids 64-90 of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), wherein said first, second
and third amino acid sequences are contiguous and in a sequential
order.
[2391] 2. An isolated polypeptide encoding for a head of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592) comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence MWVLG (SEQ
ID NO:1025) of R11723_PEA.sub.--1_P10 (SEQ ID NO:592)
[2392] 3. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO:1027) in
R11723_PEA.sub.--1_P10 (SEQ ID NO:592).
[2393] Comparison report between R11723_PEA.sub.--1_P10 (SEQ ID
NO:592) and BAC85518:
[2394] 1. An isolated chimeric polypeptide encoding for
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a first amino
acid sequence being at least 90% homologous to
MWVLGIMTFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEV MEQSA
corresponding to amino acids 24-86 of BAC85518, which also
corresponds to amino acids 1-63 of R11723_PEA.sub.--1_P10 (SEQ ID
NO:592), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID
NO:1027) corresponding to amino acids 64-90 of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), wherein said first and
second amino acid sequences are contiguous and in a sequential
order.
[2395] 2. An isolated polypeptide encoding for a tail of
R11723_PEA.sub.--1_P10 (SEQ ID NO:592), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
DRVSLCHEAGVQWNNFSTLQPLPPRLK (SEQ ID NO:1027) in
R11723_PEA.sub.--1_P10 (SEQ ID NO:592).
[2396] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2397] Variant protein R11723_PEA.sub.--1_P10 (SEQ ID NO:592) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein R11723_PEA.sub.--1_P10 (SEQ ID
NO:592) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-00967
TABLE 13 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 66 V->F
Yes
[2398] Variant protein R11723_PEA.sub.--1_P10 (SEQ ID NO:592) is
encoded by the following transcript(s): R11723_PEA.sub.--1_T20 (SEQ
ID NO:559), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
R11723_PEA.sub.--1_T20 (SEQ ID NO:559) is shown in bold; this
coding portion starts at position 434 and ends at position 703. The
transcript also has the following SNPs as listed in Table 14 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein R11723_PEA.sub.--1_P10 (SEQ ID NO:592) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-00968 TABLE 14 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 629 G->T Yes 637 G->C Yes 1307
C->T Yes
[2399] As noted above, cluster R11723 features 26 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[2400] Segment cluster R11723_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:562) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T19 (SEQ ID NO:558),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 15 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00969 TABLE
15 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T19 (SEQ 624
776 ID NO: 558) R11723_PEA.sub.--1_T5 (SEQ ID 624 776 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 658 810 NO: 561)
[2401] Segment cluster R11723_PEA.sub.--1_node.sub.--16 (SEQ ID
NO:563) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T17 (SEQ ID NO:557),
R11723_PEA.sub.--1_T19 (SEQ ID NO:558) and R11723_PEA.sub.--1_T20
(SEQ ID NO:559). Table 16 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00970 TABLE
16 Segment location on transcripts Segment starting Segment ending
Transcript name position position R11723_PEA.sub.--1_T17 (SEQ 624
1367 ID NO: 557) R11723_PEA.sub.--1_T19 (SEQ 777 1520 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 628 1371 ID NO: 559)
[2402] Segment cluster R11723_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:564) according to the present invention is supported by 45
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 17 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00971 TABLE 17 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T5 (SEQ ID 835 1008 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 869 1042 NO: 561)
[2403] Segment cluster R11723_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:565) according to the present invention is supported by 29
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 18 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00972 TABLE
18 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 1 309
ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 1 309 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 1 309 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 1 309 ID NO: 559) R11723_PEA.sub.--1_T5
(SEQ ID 1 309 NO: 560) R11723_PEA.sub.--1_T6 (SEQ ID 1 309 NO:
561)
[2404] Segment cluster R11723_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:566) according to the present invention is supported by 65
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 19 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00973 TABLE 19 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T5 (SEQ ID 1083 1569 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1117 1603 NO: 561)
[2405] Segment cluster R11723_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:567) according to the present invention is supported by 70
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556,
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 20 below describes the starting and ending
position of this segment on each transcript (it should be noted
that these transcripts show alternative polyadenylation).
TABLE-US-00974 TABLE 20 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T15 (SEQ 1060 1295 ID NO: 556)
R11723_PEA.sub.--1_T5 (SEQ ID 1978 2213 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 2012 2247 NO: 561)
[2406] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2407] Segment cluster R11723_PEA.sub.--1_node.sub.--10 (SEQ ID
NO:568) according to the present invention is supported by 38
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556)
R11723_PEA.sub.--1_T17 (SEQ ID NO:557),
R11723.sub.--1_PEA.sub.--1_T19 (SEQ ID NO:558),
R11723_PEA.sub.--1_T20 (SEQ ID NO:559), R11723_PEA.sub.--1_T5 (SEQ
ID NO:560) and R11723_PEA.sub.--1 T6 (SEQ ID NO:561). Table 21
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00975 TABLE 21 Segment location on
transcripts Segment Segment Transcript name starting position
ending position R11723_PEA.sub.--1_T15 (SEQ 486 529 ID NO: 556)
R11723_PEA.sub.--1_T17 (SEQ 486 529 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 486 529 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 486 529 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 486 529 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 520 563 NO: 561)
[2408] Segment cluster R11723_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:569) according to the present invention is supported by 42
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556,
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1.sub.--T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 22 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00976 TABLE
22 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 530
623 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 530 623 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 530 623 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 530 623 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 530 623 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 564 657 NO: 561)
[2409] Segment cluster R11723_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:570) according to the present invention can be found in the
following transcript(s): R11723_PEA.sub.--1_T20 (SEQ ID NO:559).
Table 23 below describes the starting and ending position of this
segment on each transcript. TABLE-US-00977 TABLE 23 Segment
location on transcripts Segment Segment Transcript name starting
position ending position R11723_PEA.sub.--1_T20 (SEQ 624 627 ID NO:
559)
[2410] Segment cluster R11723_PEA.sub.--1_node.sub.--18 (SEQ ID
NO:571) according to the present invention is supported by 40
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 24 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00978 TABLE
24 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 624
681 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 777 834 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 811 868 NO: 561)
[2411] Segment cluster R11723_PEA.sub.--1_node.sub.--20 (SEQ ID
NO:572) according to the present invention can be found in the
following transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 25 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00979 TABLE 25 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T5 (SEQ ID 1009 1019 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1043 1053 NO: 561)
[2412] Segment cluster R11723_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:573) according to the present invention is supported by 36
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 26 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00980 TABLE 26 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T5 (SEQ ID 1020 1082 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1054 1116 NO: 561)
[2413] Segment cluster R11723_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:574) according to the present invention is supported by 39
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and
R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 27 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-00981 TABLE 27 Segment location on transcripts Segment
Segment Transcript name starting position ending position
R11723_PEA.sub.--1_T5 (SEQ ID 1570 1599 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1604 1633 NO: 561)
[2414] Segment cluster R11723_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:575) according to the present invention is supported by 51
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 28 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00982 TABLE
28 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 682
765 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1600 1683 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1634 1717 NO: 561)
[2415] Segment cluster R11723_PEA.sub.--1_node.sub.--25 (SEQ ID
NO:576) according to the present invention is supported by 54
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 29 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00983 TABLE
29 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 766
791 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1684 1709 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1718 1743 NO: 561)
[2416] Segment cluster R11723_PEA.sub.--1_node.sub.--26 (SEQ ID
NO:577) according to the present invention is supported by 62
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00984 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 792
904 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1710 1822 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1744 1856 NO: 561)
[2417] Segment cluster R11723_PEA.sub.--1_node.sub.--27 (SEQ ID
NO:578) according to the present invention is supported by 67
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00985 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 905
986 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1823 1904 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1857 1938 NO: 561)
[2418] Segment cluster R11723_PEA.sub.--1_node.sub.--28 (SEQ ID
NO:579) according to the present invention can be found in the
following transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 32 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00986 TABLE
32 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 987
1010 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1905 1928 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1939 1962 NO: 561)
[2419] Segment cluster R11723_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:580) according to the present invention is supported by 69
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 33 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00987 TABLE
33 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 1011
1038 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1929 1956 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1963 1990 NO: 561)
[2420] Segment cluster R11723_PEA.sub.--1_node.sub.--3 (SEQ ID
NO:581) according to the present invention can be found in the
following transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA_T19 (SEQ ID
NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 34 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00988 TABLE
34 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1 T15 (SEQ 310
319 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 310 319 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 310 319 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 310 319 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 310 319 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 310 319 NO: 561)
[2421] Segment cluster R11723_PEA.sub.--1_node.sub.--30 (SEQ ID
NO:582) according to the present invention can be found in the
following transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 35 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00989 TABLE
35 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 1039
1059 ID NO: 556) R11723_PEA.sub.--1_T5 (SEQ ID 1957 1977 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 1991 2011 NO: 561)
[2422] Segment cluster R11723_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:583) according to the present invention is supported by 25
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 36 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00990 TABLE
36 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 320
371 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 320 371 ID NO: 557)
R11723_PEA.sub.--1 T19 (SEQ 320 371 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 320 371 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 320 371 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 320 371 NO: 561)
[2423] Segment cluster R11723_PEA.sub.--1_node.sub.--5 (SEQ ID
NO:584) according to the present invention is supported by 26
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 37 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00991 TABLE
37 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 372
414 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 372 414 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 372 414 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 372 414 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 372 414 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 372 414 NO: 561)
[2424] Segment cluster R11723_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:585) according to the present invention is supported by 27
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 38 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00992 TABLE
38 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 415
446 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 415 446 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 415 446 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 415 446 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 415 446 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 415 446 NO: 561)
[2425] Segment cluster R11723_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:586) according to the present invention is supported by 29
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T15 (SEQ ID NO:556),
R11723_PEA.sub.--1_T17 (SEQ ID NO:557), R11723_PEA.sub.--1_T19 (SEQ
ID NO:558), R11723_PEA.sub.--1_T20 (SEQ ID NO:559),
R11723_PEA.sub.--1_T5 (SEQ ID NO:560) and R11723_PEA.sub.--1_T6
(SEQ ID NO:561). Table 39 below describes the starting and ending
position of this segment on each transcript. TABLE-US-00993 TABLE
39 Segment location on transcripts Segment Segment Transcript name
starting position ending position R11723_PEA.sub.--1_T15 (SEQ 447
485 ID NO: 556) R11723_PEA.sub.--1_T17 (SEQ 447 485 ID NO: 557)
R11723_PEA.sub.--1_T19 (SEQ 447 485 ID NO: 558)
R11723_PEA.sub.--1_T20 (SEQ 447 485 ID NO: 559)
R11723_PEA.sub.--1_T5 (SEQ ID 447 485 NO: 560)
R11723_PEA.sub.--1_T6 (SEQ ID 447 485 NO: 561)
[2426] Segment cluster R11723_PEA.sub.--1_node.sub.--8 (SEQ ID
NO:587) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): R11723_PEA.sub.--1_T6 (SEQ ID NO:561). Table 40
below describes the starting and ending position of this segment on
each transcript. TABLE-US-00994 TABLE 40 Segment location on
transcripts Segment Segment Transcript name starting position
ending position R11723_PEA.sub.--1_T6 (SEQ ID 486 519 NO: 561)
[2427] Variant protein alignment to the previously known
protein:
[2428] Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q8IXM0
[2429] Sequence Documentation:
[2430] Alignment of: R11723_PEA.sub.--1_P6 (SEQ ID
NO:589).times.Q8IXM0.
[2431] Alignment segment 1/1: TABLE-US-00995 Quality: 1128.00
Escore: 0 Matching length: 112 Total length: 112 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00086 Alignment: TABLE-US-00996 111
MYAQALLVVGVLQRQAAAQHLHEHPPKLLRGHRVQERVDDRAEVEKRLRE 160
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MYAQALLVVGVLQRQAAAQHLHEHPPKLLRGHRVQERVDDRAEVEKRLRE 50 161
GEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQCHNNQPWADTSRRE 210
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
GEEDHVRPEVGPRPVVLGFGRSHDPPNLVGHPAYGQCHNNQPWADTSRRE 100 211
RQRKEKHSMRTQ 222 |||||||||||| 101 RQRKEKHSMRTQ 112
[2432] Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q96AC2 (SEQ ID NO:
886)
[2433] Sequence Documentation:
[2434] Alignment of: R11723_PEA.sub.--1_P6 (SEQ ID
NO:589).times.Q96AC2 (SEQ ID NO: 886).
[2435] Alignment segment 1/1: TABLE-US-00997 Quality: 835.00
Escore: 0 Matching length: 83 Total length: 83 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00087 Alignment: TABLE-US-00998 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 83
||||||||||||||||||||||||||||||||| 51
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 83
[2436] Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:Q8N2G4
[2437] Sequence Documentation:
[2438] Alignment of: R11723_PEA.sub.--1_P6 (SEQ ID
NO:589).times.Q8N2G4.
[2439] Alignment segment 1/1: TABLE-US-00999 Quality: 835.00
Escore: 0 Matching length: 83 Total length: 83 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00088 Alignment: TABLE-US-01000 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGTAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 83
||||||||||||||||||||||||||||||||| 51
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 83
[2440] Sequence name: /tmp/gp6eQTLWqk/mFtjUpUzhb:BAC85518
[2441] Sequence Documentation:
[2442] Alignment of: R11723_PEA.sub.--1_P6 (SEQ ID
NO:589).times.BAC85518.
[2443] Alignment segment 1/1: TABLE-US-01 001 Quality: 835.00
Escore: 0 Matching length: 83 Total length: 83 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00089 Alignment: TABLE-US-01002 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 24
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 73 51
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 83
||||||||||||||||||||||||||||||||| 74
QDMCQKEVMEQSAGIMYRKSCASSAACLIASAG 106
[2444] Sequence name: /tmpNXjdFlzdBX/bexTxThOTh:Q96AC2 (SEQ ID NO:
886)
[2445] Sequence Documentation:
[2446] Alignment of: R11723_PEA.sub.--1_P7 (SEQ ID
NO:590).times.Q96AC2 (SEQ ID NO: 886).
[2447] Alignment segment 1/1: TABLE-US-01003 Quality: 654.00
Escore: 0 Matching length: 64 Total length: 64 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00090 Alignment: TABLE-US-01004 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSAG 64 |||||||||||||| 51 QDMCQKEVMEQSAG 64
[2448] Sequence name: /tmpNXjd FlzdBX/bexTxThOTh :Q8N2G4
[2449] Sequence Documentation:
[2450] Alignment of: R11723_PEA.sub.--1_P7 (SEQ ID
NO:590).times.Q8N2G4.
[2451] Alignment segment 1/1: TABLE-US-01 005 Quality: 654.00
Escore: 0 Matching length: 64 Total length: 64 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00091 Alignment: TABLE-US-01006 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSAG 64 |||||||||||||| 51 QDMCQKEVMEQSAG 64
[2452] Sequence name: /tmp/VXjdFlzdBX/bexTxTh0Th:BAC85273
[2453] Sequence Documentation:
[2454] Alignment of: R11723_PEA.sub.--1_P7 (SEQ ID
NO:590).times.BAC85273.
[2455] Alignment segment 1/1: TABLE-US-01007 Quality: 600.00
Escore: 0 Matching length: 59 Total length: 59 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00092 Alignment: TABLE-US-01008 6
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQ 55
|||||||||||||||||||||||||||||||||||||||||||||||||| 22
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQ 71 56 KEVMEQSAG
64 ||||||||| 72 KEVMEQSAG 80
[2456] Sequence name: /tmpNXjdFlzdBX/bexTxThOTh:BAC85518
[2457] Sequence Documentation:
[2458] Alignment of: R11723_PEA.sub.--1_P7 (SEQ ID
NO:590).times.BAC85518.
[2459] Alignment segment 1/1: TABLE-US-01009 Quality: 654.00
Escore: 0 Matching length: 64 Total length: 64 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00093 Alignment: TABLE-US-01010 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 24
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 73 51
QDMCQKEVMEQSAG 64 |||||||||||||| 74 QDMCQKEVMEQSAG 87
[2460] Sequence name: /tmp/OLMSexEmlh/pc7Z7Xm1YR:Q96AC2 (SEQ ID NO:
886)
[2461] Sequence Documentation:
[2462] Alignment of: R11723_PEA.sub.--1_P10 (SEQ ID
NO:592).times.Q96AC2 (SEQ ID NO: 886).
[2463] Alignment segment 1/1: TABLE-US-01011 Quality: 645.00
Escore: 0 Matching length: 63 Total length: 63 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00094 Alignment: TABLE-US-01012 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSA 63 ||||||||||||| 51 QDMCQKEVMEQSA 63
[2464] Sequence name: /tmp/OLMSexEmlh/pc7Z7Xm1YR:Q8N2G4
[2465] Sequence Documentation:
[2466] Alignment of: R11723_PEA.sub.--1_P10 (SEQ ID
NO:592).times.Q8N2G4.
[2467] Alignment segment 1/1: TABLE-US-01 013 Quality: 645.00
Escore: 0 Matching length: 63 Total length: 63 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00095 Alignment: TABLE-US-01014 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSA 63 ||||||||||||| 51 QDMCQKEVMEQSA 63
[2468] Sequence name: /tmp/OLMSexEmlh/pc7Z7Xm1YR:BAC85273
[2469] Sequence Documentation:
[2470] Alignment of: R11723_PEA.sub.--1_P10 (SEQ ID
NO:592).times.BAC85273.
[2471] Alignment segment 1/1: TABLE-US-01015 Quality: 591.00
Escore: 0 Matching length: 58 Total length: 58 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00096 Alignment: TABLE-US-01016 . . . 6
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQ 55
|||||||||||||||||||||||||||||||||||||||||||||||||| 22
IAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQ 71 56 KEVMEQSA
63 |||||||| 72 KEVMEQSA 79
[2472] Sequence name: /tmp/OLMSexEmlh/pc7Z7Xm1YR:BAC85518.
[2473] Alignment Documentation:
[2474] Alignment of: R11723_PEA.sub.--1_P10 (SEQ ID
NO:592).times.BAC85518.
[2475] Alignment segment 1/1: TABLE-US-01017 Quality: 645.00
Escore: 0 Matching length: 63 Total length: 63 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00097 Alignment: TABLE-US-01018 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 24
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 73 51
QDMCQKEVMEQSA 63 ||||||||||||| 74 QDMCQKEVMEQSA 86
[2476] Alignment of: R11723_PEA.sub.--1.sub.--P13 (SEQ ID
NO:591).times.Q96AC2 (SEQ ID NO: 886).
[2477] Alignment segment 1/1: TABLE-US-01019 Quality: 645.00
Escore: 0 Matching length: 63 Total length: 63 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00098 Alignment: TABLE-US-01020 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MWVLGIAATFCGLFLLPGFALQIQCYQCEEFQLNNDCSSPEFIVNCTVNV 50 51
QDMCQKEVMEQSA 63 ||||||||||||| 51 QDMCQKEVMEQSA 63
Expression of R11723 Transcripts Which are Detectable by Amplicon
as Depicted in Sequence Name R11723 Seg13 (SEQ ID NO:891) in Normal
and Cancerous Breast Tissues Expression of transcripts detectable
by or according to seg13, R11723 seg13 amplicon(s) (SEQ ID NO:891)
and R11723 seg13F (SEQ ID NO:889) and R11723 seg13R (SEQ ID NO:890)
primers was measured by real time PCR. It should be noted that the
variants of this cluster are variants of the hypothetical protein
PSEC0181 (referred to herein as "PSEC"). In parallel the expression
of four housekeeping genes PBGD (GenBank Accession No. BC019323
(SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1
(GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)), G6PD (GenBank Accession
No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ ID NO:921))
was measured similarly. For each RT sample, the expression of the
above amplicon was normalized to the geometric mean of the
quantities of the housekeeping genes. The normalized quantity of
each RT sample was then divided by the median of the quantities of
the normal post-mortem (PM) samples (Sample Nos. 56-60, 63-67,
Table 1, "Tissue samples in testing panel" above), to obtain a
value of fold up-regulation for each sample relative to median of
the normal PM samples.
[2478] FIG. 39 is a histogram showing over expression of the
above-indicated transcripts in cancerous breast samples relative to
the normal samples.
[2479] As is evident from FIG. 39, the expression of transcripts
detectable by the above amplicon(s) in cancer samples was higher
than in the non-cancerous samples (Sample Nos. 56-60, 63-67 Table
1, Tissue samples in testing panel). Notably an over-expression of
at least 5 fold was found in 5 out of 28 adenocarcinoma
samples.
[2480] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: R11723 seg13F
forward primer (SEQ ID NO:889); and R11723 seg13R reverse primer
(SEQ ID NO:890).
[2481] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: R11723 seg13 (SEQ ID NO:891). TABLE-US-01 021 R11 723seg1
3F-ACACTAAAAGAACAAACACCTTGCTC (SEQ ID NO:889)
R11723seg13R-TCCTCAGAAGGCACATGAAAGA (SEQ ID NO:890) R11723seg13
amplicon: ACACTAAAAGAACAAACACCTTGCTCTTCGAGATGAGACATTTTGCCAAGCAGTTG
(SEQ ID NO:891)
ACCACTTAGTTCTCAAGAAGCAACTATCTCTTTCATGTGCCTTCTGAGGA
Expression of R11723 Transcripts Which are Detectable by Amplicon
as Depicted in Sequence Name R11723 Seg13 (SEQ ID NO:891) in
Different Normal Tissues
[2482] Expression of R11723 transcripts detectable by or according
to R11723seg13 amplicon (SEQ ID NO:891) and R11723seg13F (SEQ ID
NO:889) R11723seg13R (SEQ ID NO:890) was measured by real time PCR.
In parallel the expression of four housekeeping genes RPL19
(GenBank Accession No. NM.sub.--000981 (SEQ ID NO:934); RPL19
amplicon (SEQ ID NO:937)), TATA box (GenBank Accession No.
NM.sub.--003194 (SEQ ID NO:938); TATA amplicon (SEQ ID NO:941)),
UBC (GenBank Accession No. BC000449 (SEQ ID NO:942);
amplicon--Ubiquitin-amplicon (SEQ ID NO:945) and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the ovary samples
(Sample Nos. 18-20 Table 2 "Tissue samples in normal panel" above),
to obtain a value of relative expression of each sample relative to
median of the ovary samples. Primers and amplicon are as above.
[2483] The results are presented in FIG. 40, demonstrating the
expression of R11723 transcripts which are detectable by amplicon
as depicted in sequence name R11723seg13 (SEQ ID NO:891) in
different normal tissues.
Expression of R11723 Transcripts, Which are Detectable by Amplicon
as Depicted in Sequence Name R11723 Junc11-18 (SEQ ID NO:894) in
Normal and Cancerous Breast Tissues
[2484] Expression of transcripts detectable by or according to
junc1'-18, R11723 junc 11-18 amplicon(s) (SEQ ID NO:894) and R11723
junc1'-18F (SEQ ID NO:892) and R11723 junc1'-18R (SEQ ID NO:893)
primers was measured by real time PCR (this junction and hence the
amplicon are found in the previous known protein, also termed the
"wild type" or WT protein, for which the sequence is given herein;
the protein is also called "PSEC"). Use of the known protein (WT
protein) for detection of breast cancer, alone or in combination
with one or more variants of this cluster and/or of any other
cluster and/or of any known marker, also comprises an embodiment of
the present invention. In parallel the expression of four
housekeeping genes PBGD (GenBank Accession No. BC019323 (SEQ ID
NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)), HPRT1 (GenBank
Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933)), SDHA (GenBank Accession
No. NM.sub.--004168 (SEQ ID NO:922); amplicon--SDHA-amplicon (SEQ
ID NO:925), and G6PD (GenBank Accession No. NM.sub.--000402 (SEQ ID
NO:918); G6PD-amplicon (SEQ ID NO:921)), was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the normal
post-mortem (PM) samples (Sample Nos. 56-60, 63-67, Table 1: Tissue
samples in testing panel, above), to obtain a value of fold
up-regulation for each sample relative to median of the normal PM
samples.
[2485] FIG. 41A is a histogram showing over expression of the
above-indicated transcripts in cancerous breast samples relative to
the normal samples.
[2486] As is evident from FIG. 41A, the expression of transcripts
detectable by the above amplicon in a few cancer samples was higher
than in the non-cancerous samples (Sample Nos. 56-60, 63-67, Table
5: "Tissue samples in breast cancer testing panel"). Notably an
over-expression of at least 5 fold was found in 5 out of 28
adenocarcinoma samples.
[2487] Primer pairs are also optionally and preferably encompassed
within the present invention; for example, for the above
experiment, the following primer pair was used as a non-limiting
illustrative example only of a suitable primer pair: R11723
junc11-18F forward primer (SEQ ID NO:892); and R1723 junc11-18R
reverse primer (SEQ ID NO:893).
[2488] The present invention also preferably encompasses any
amplicon obtained through the use of any suitable primer pair; for
example, for the above experiment, the following amplicon was
obtained as a non-limiting illustrative example only of a suitable
amplicon: R11723 junc11-18 (SEQ ID NO:894). TABLE-US-01022
R11723junc11-18F-AGTGATGGAGCAAAGTGCCG (SEQ ID NO:892) R11723
junc1'-18R-CAGCAGCTGATGCAAACTGAG (SEQ ID NO:893)
R11723junc11-18-AGTGATGGAGCAAAGTGCCGGGATCATGTACCGCAAGTCCTGTGCATCATCAGCGG
(SEQ ID NO:894)
CCTGTCTCATCGCCTCTGCCGGGTACCAGTCCTTCTGCTCCCCAGGGAAACTGAACT
CAGTTTGCATCAGCTGCTG
Expression of R11723 Transcripts, Which were Detected by Amplicon
as Depicted in the Sequence Name R11723 junc1-18 (SEQ ID NO:894) in
Different Normal Tissues
[2489] Expression of R11723 transcripts detectable by or according
to R11723seg13 amplicon (SEQ ID NO:894) and R11723 junc11-18F (SEQ
ID NO:892) R1723junc11-18R (SEQ ID NO:893) was measured by real
time PCR (as described above, this junction and hence the amplicon
are found in the previous known protein, also termed the "wild
type" or WT protein, for which the sequence is given herein; the
protein is also called "PSEC"). In parallel the expression of four
housekeeping genes RPL19 (GenBank Accession No. NM.sub.--000981
(SEQ ID NO:934); RPL19 amplicon (SEQ ID NO:937)), TATA box (GenBank
Accession No. NM.sub.--003194 (SEQ ID NO:938); TATA amplicon (SEQ
ID NO:941)), UBC (GenBank Accession No. BC000449 (SEQ ID NO:942);
amplicon--Ubiquitin-amplicon (SEQ ID NO:945)) and SDHA (GenBank
Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicon was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample was
then divided by the median of the quantities of the ovary samples
(Sample Nos. 18-20, Table 2: Tissue samples in normal panel,
above), to obtain a value of relative expression of each sample
relative to median of the ovary samples. FIG. 41B shows the level
of expression of this transcript. Primers and amplicon are as for
the example above.
[2490] The variant transcript expression pattern for this cluster
is similar to the wild type transcript expression. However, in some
cases (e.g. ovary cancer) over expression of the variant seems to
be higher (for example, with regard to R11723_PEA.sub.--1.sub.--T5
(SEQ ID NO:560)).
Description for Cluster T46984
[2491] Cluster T46984 features 21 transcript(s) and 49 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-01023 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. T46984_PEA.sub.--1_T2 593 T46984_PEA.sub.--1_T3 594
T46984_PEA.sub.--1_T12 595 T46984_PEA.sub.--1_T13 596
T46984_PEA.sub.--1_T14 597 T46984_PEA.sub.--1_T15 598
T46984_PEA.sub.--1_T19 599 T46984_PEA.sub.--1_T23 600
T46984_PEA.sub.--1_T27 601 T46984_PEA.sub.--1_T32 602
T46984_PEA.sub.--1_T34 603 T46984_PEA.sub.--1_T35 604
T46984_PEA.sub.--1_T40 605 T46984_PEA.sub.--1_T42 606
T46984_PEA.sub.--1_T43 607 T46984_PEA.sub.--1_T46 608
T46984_PEA.sub.--1_T47 609 T46984_PEA.sub.--1_T48 610
T46984_PEA.sub.--1_T51 611 T46984_PEA.sub.--1_T52 612
T46984_PEA.sub.--1_T54 613
[2492] TABLE-US-01024 TABLE 2 Segments of interest Segment Name
Sequence ID No. T46984_PEA.sub.--1_node.sub.--2 614
T46984_PEA.sub.--1_node.sub.--4 615 T46984_PEA.sub.--1_node.sub.--6
616 T46984_PEA.sub.--1_node.sub.--12 617
T46984_PEA.sub.--1_node.sub.--14 618
T46984_PEA.sub.--1_node.sub.--25 619
T46984_PEA.sub.--1_node.sub.--29 620
T46984_PEA.sub.--1_node.sub.--34 621
T46984_PEA.sub.--1_node.sub.--46 622
T46984_PEA.sub.--1_node.sub.--47
623T46984_PEA.sub.--1_node.sub.--52 624T46984_PEA.sub.--1_node65
625 T46984_PEA.sub.--1_node.sub.--69 626
T46984_PEA.sub.--1_node.sub.--75 627
T46984_PEA.sub.--1_node.sub.--86 628
T46984_PEA.sub.--1_node.sub.--9 629
T46984_PEA.sub.--1_node.sub.--13 630
T46984_PEA.sub.--1_node.sub.--19 631
T46984_PEA.sub.--1_node.sub.--21 632
T46984_PEA.sub.--1_node.sub.--22 633
T46984_PEA.sub.--1_node.sub.--26 634
T46984_PEA.sub.--1_node.sub.--28 635
T46984_PEA.sub.--1_node.sub.--31 636
T46984_PEA.sub.--1_node.sub.--32 637
T46984_PEA.sub.--1_node.sub.--38 638
T46984_PEA.sub.--1_node.sub.--39 639
T46984_PEA.sub.--1_node.sub.--40 640
T46984_PEA.sub.--1_node.sub.--42 641
T46984_PEA.sub.--1_node.sub.--43 642
T46984_PEA.sub.--1_node.sub.--48 643
T46984_PEA.sub.--1_node.sub.--49 644
T46984_PEA.sub.--1_node.sub.--50 645
T46984_PEA.sub.--1_node.sub.--51 646
T46984_PEA.sub.--1_node.sub.--53 647
T46984_PEA.sub.--1_node.sub.--54 648
T46984_PEA.sub.--1_node.sub.--55 649
T46984_PEA.sub.--1_node.sub.--57 650
T46984_PEA.sub.--1_node.sub.--60 651
T46984_PEA.sub.--1_node.sub.--62 652
T46984_PEA.sub.--1_node.sub.--66 653
T46984_PEA.sub.--1_node.sub.--67 654
T46984_PEA.sub.--1_node.sub.--70 655
T46984_PEA.sub.--1_node.sub.--71 656
T46984_PEA.sub.--1_node.sub.--72 657
T46984_PEA.sub.--1_node.sub.--73 658
T46984_PEA.sub.--1_node.sub.--74 659
T46984_PEA.sub.--1_node.sub.--83 660
T46984_PEA.sub.--1_node.sub.--84 661
T46984_PEA.sub.--1_node.sub.--85 662
[2493] TABLE-US-01025 TABLE 3 Proteins of interest Sequence ID
Protein Name No. Corresponding Transcript(s) T46984_PEA.sub.--1_P2
664 T46984_PEA.sub.--1_T2 (SEQ ID NO: 593); T46984_PEA.sub.--1_T12
(SEQ ID NO: 595); T46984_PEA.sub.--1_T23 (SEQ ID NO: 600)
T46984_PEA.sub.--1_P3 665 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594);
T46984_PEA.sub.--1_T19 (SEQ ID NO: 599) T46984_PEA.sub.--1_P10 666
T46984_PEA.sub.--1_T13 (SEQ ID NO: 596) T46984_PEA.sub.--1_P11 667
T46984_PEA.sub.--1_T14 (SEQ ID NO: 597) T46984_PEA.sub.--1_P12 668
T46984_PEA.sub.--1_T15 (SEQ ID NO: 598) T46984_PEA.sub.--1_P21 669
T46984_PEA.sub.--1_T27 (SEQ ID NO: 601) T46984_PEA.sub.--1_P27 670
T46984_PEA.sub.--1_T34 (SEQ ID NO: 603) T46984_PEA.sub.--1_P32 671
T46984_PEA.sub.--1_T40 (SEQ ID NO: 605) T46984_PEA.sub.--1_P34 672
T46984_PEA.sub.--1_T42 (SEQ ID NO: 606) T46984_PEA.sub.--1_P35 673
T46984_PEA.sub.--1_T43 (SEQ ID NO: 607) T46984_PEA.sub.--1_P38 674
T46984_PEA.sub.--1_T47 (SEQ ID NO: 609) T46984_PEA.sub.--1_P39 675
T46984_PEA.sub.--1_T48 (SEQ ID NO: 610) T46984_PEA.sub.--1_P45 676
T46984_PEA.sub.--1_T32 (SEQ ID NO: 602) T46984_PEA.sub.--1_P46 677
T46984_PEA.sub.--1_T35 (SEQ ID NO: 604)
[2494] These sequences are variants of the known protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (SwissProt accession identifier RIB2_HUMAN;
known also according to the synonyms EC 2.4.1.119; Ribophorin II;
RPN-II; RIBIIR), SEQ ID NO: 663, referred to herein as the
previously known protein.
[2495] Protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663) is
known or believed to have the following function(s): Essential
subunit of N-oligosaccharyl transferase enzyme which catalyzes the
transfer of a high mannose oligosaccharide from a lipid-linked
oligosaccharide donor to an asparagine residue within an
Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. The
sequence for protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663) is
given at the end of the application, as
"Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (SEQ ID NO:663) amino acid sequence". Known
polymorphisms for this sequence are as shown in Table 4.
TABLE-US-01 026 TABLE 4 Amino acid mutations for Known Protein SNP
position(s) on amino acid sequence Comment 197 V->L 201 F->C
260 A->S 423 V->M
[2496] Protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)
localization is believed to be Type I membrane protein. Endoplasmic
reticulum.
[2497] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: protein
modification, which are annotation(s) related to Biological
Process; oligosaccharyl transferase;
dolichyl-diphosphooligosaccharide-protein glycosyltransferase;
transferase, which are annotation(s) related to Molecular Function;
and oligosaccharyl transferase complex; integral membrane protein,
which are annotation(s) related to Cellular Component.
[2498] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[2499] Cluster T46984 can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 42 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[2500] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 42 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: epithelial malignant tumors, a mixture of
malignant tumors from different tissues, breast malignant tumors,
ovarian carcinoma and pancreas carcinoma. TABLE-US-01027 TABLE 5
Normal tissue distribution Name of Tissue Number adrenal 240
bladder 287 bone 592 brain 145 colon 157 epithelial 144 general 163
head and neck 50 kidney 139 liver 156 lung 155 lymph nodes 194
breast 105 bone marrow 62 muscle 62 ovary 0 pancreas 72 prostate
201 skin 91 stomach 219 T cells 0 Thyroid 0 uterus 200
[2501] TABLE-US-01028 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 adrenal 6.3e-01
5.4e-01 6.2e-01 0.8 2.5e-01 1.0 bladder 5.4e-01 5.9e-01 3.0e-01 1.0
6.5e-01 0.7 bone 3.9e-01 3.7e-01 9.8e-01 0.4 9.9e-01 0.4 brain
3.3e-01 2.9e-01 1.4e-01 1.2 2.0e-01 1.0 colon 8.6e-02 5.9e-02
2.6e-01 1.3 2.1e-03 1.4 epithelial 5.3e-05 6.2e-07 2.8e-08 1.9
3.4e-21 2.4 general 1.0e-04 7.3e-08 9.3e-12 1.7 8.0e-33 2.0 head
and neck 4.5e-01 5.4e-01 1 0.8 7.5e-01 0.9 kidney 6.6e-01 6.5e-01
3.2e-01 1.2 5.3e-02 1.5 liver 5.5e-01 5.6e-01 6.5e-01 1.0 1.2e-01
1.4 lung 3.0e-01 1.7e-01 1.5e-01 1.4 6.0e-02 1.4 lymph nodes
2.9e-01 5.5e-01 2.9e-01 0.8 4.3e-01 1.0 breast 2.4e-02 5.8e-03
3.7e-02 2.2 1.7e-04 2.7 bone marrow 7.1 e-01 7.5e-01 1 0.3 1.2e-02
1.8 muscle 5.0e-01 3.7e-01 4.7e-01 1.5 2.1e-08 1.3 ovary 1.6e-02
7.0e-03 1.5e-02 6.1 4.8e-06 7.1 pancreas 1.4e-01 5.4e-02 2.2e-05
2.9 2.4e-07 3.9 prostate 3.4e-01 1.9e-01 2.2e-01 1.2 1.4e-01 1.3
skin 3.7e-01 1.5e-01 4.2e-02 2.4 1.1e-04 1.9 stomach 6.1 e-01
1.4e-01 7.3e-01 0.4 6.1 e-02 1.6 T cells 1 6.7e-01 1 1.0 5.2e-01
1.8 Thyroid 4.8e-02 4.8e-02 2.0e-01 3.4 2.0e-01 3.4 uterus 2.3e-01
1.3e-01 2.2e-02 1.5 5.0e-02 1.4
[2502] As noted above, cluster T46984 features 21 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (SEQ ID NO:663). A description of each
variant protein according to the present invention is now
provided.
[2503] Variant protein T46984_PEA.sub.--1_P2 (SEQ ID NO:664)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T2 (SEQ ID NO:593). An alignment is given to the
known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2504] Comparison report between T46984_PEA.sub.--1_P2 (SEQ ID
NO:664) and RIB2_HUMAN (SEQ ID NO:663):
[2505] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P2 (SEQ ID NO:664), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-498 of T46984_PEA.sub.--1_P2 (SEQ ID NO:664), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence VCA corresponding to amino acids 499-501 of
T46984_PEA.sub.--1_P2 (SEQ ID NO:664), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2506] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2507] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P2 (SEQ ID NO:664), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 7 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01029 TABLE 7
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 106 yes
106
[2508] Variant protein T46984_PEA.sub.--1_P2 (SEQ ID NO:664) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T2 (SEQ
ID NO:593), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T2 (SEQ ID NO:593) is shown in bold; this coding
portion starts at position 316 and ends at position 1818. The
transcript also has the following SNPs as listed in Table 8 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984_PEA.sub.--1_P2 (SEQ ID NO:664) sequence provides
support for the deduced sequence of the variant protein according
to the present invention). TABLE-US-01030 TABLE 8 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 28 G->C No 173 G->C Yes 256 C->T Yes
274 G->C Yes 325 C->No 389 C->G Yes 610 G->A Yes 718
T->No 724 C->No 844 C->T Yes 857->G No 885 C->No
897->G No 1002 G->A No 1048A->No 1048A->G No
1068A->C No 1076 G->A Yes 1187A->No 1187A->C No
1220A->G No 1220A->T No 1254T->G No 1291 A->C No
1293C->G No 1303G->A No 1376G->T Yes 1588A->C No
1618T->No1618T->C No 1660T->No 1693 A->C No 1693
A->T No 2099 G->A Yes 2124 C->G Yes 2124 C->T Yes 2133
A->G Yes 2501 C->T Yes 2617 G->T Yes 2683 C->T Yes 2741
G->A Yes 2940 T->No 3024 G->A Yes 3158 C->No 3158
C->A No 3165 C->No 3169 G->No 3354 C->A No 3374 T->C
Yes 3468 C->T No 3501 A->C No 3513 A->T No 3528 G->A
Yes 3534->A No 3543 A->G No 3568 T->G No 3582 T->A No
3582 T->G No 3682->C No 3691 T->No 3750 A->C No
[2509] Variant protein T46984_PEA.sub.--1_P3 (SEQ ID NO:665)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T3 (SEQ ID NO:594). An alignment is given to the
known protein (Dolichyl-diphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2510] Comparison report between T46984_PEA.sub.--1_P3 (SEQ ID
NO:665) and RIB2_HUMAN (SEQ ID NO:663):
[2511] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVMLSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVMTYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQ corresponding to amino acids 1-433 of RIB2_HUMAN
(SEQ ID NO:663), which also corresponds to amino acids 1-433 of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ICHIWKLIFLP (SEQ ID NO:947) corresponding to amino acids 434-444 of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2512] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ICHIWKLIFLP (SEQ ID NO:947) in T46984_PEA.sub.--1_P3 (SEQ ID
NO:665).
[2513] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2514] Variant protein T46984_PEA.sub.--1_P3 (SEQ ID NO:665) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 9, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P3 (SEQ ID
NO:665) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01031
TABLE 9 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s)
[2515] Previously known SNP? 4 P->No 25 P->R Yes 99 G->R
Yes 135 F->No 137 L->No 190 R->No 245 N->No 245 N->D
No 251 E->D No 254 S->N Yes 291 Q->No 291 Q->P No 302
Q->R No 302 Q->L No 326 T->P No 330 D->N No 354 G->V
Yes 425 T->P No
[2516] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P3 (SEQ ID NO:665), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 10 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01032 TABLE 10
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 106 yes
106
[2517] Variant protein T46984_PEA.sub.--1_P3 (SEQ ID NO:665) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T3 (SEQ
ID NO:594), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T3 (SEQ ID NO:594) is shown in bold; this coding
portion starts at position 316 and ends at position 1647. The
transcript also has the following SNPs as listed in Table 11 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984_PEA.sub.--1_P3 (SEQ ID NO:665) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01033 TABLE 11 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 28 G->C No 173 G->C Yes 256 C->T Yes
274 G->C Yes 325 C->No 389 C->G Yes 610 G->A Yes 718
T->No 724 C->No 844 C->T Yes 857->G No 885 C->No
897->G No 1002 G->A No 1048A->No 1048A->G No
1068A->C No 1076 G->A Yes 1187A->No 1187 A->C No 1220
A->G No 1220 A->T No 1254 T->G No 1291 A->C No 1293
C->G No 1303 G->A No 1376 G->T Yes 1588 A->C No 1784
C->T Yes 1959 G->A Yes 2112 G->A Yes 2137 C->G Yes 2246
T->No 2246 T->C No 2288 T->No 2321 A->C No 2321 A->T
No 2552 C->No 2552 C->A No 2559 C->No 2563 G->No 2748
C->A No 2768 T->C Yes 2862 C->T No 2895 A->C No 2907
A->T No 2922 G->A Yes 2928->A No 2937 A->G No 2962
T->G No 2976 T->A No 2976 T->G No 3076->C No 3085
T->No 3144 A->C No
[2518] Variant protein T46984_PEA.sub.--1_P10 (SEQ ID NO:666)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T13 (SEQ ID NO:596). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2519] Comparison report between T46984_PEA.sub.--1_P10 (SEQ ID
NO:666) and RIB2_HUMAN SEQ ID NO:663):
[2520] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P10 (SEQ ID NO:666), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNV corresponding to amino acids 1-498 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-498 of T46984_PEA.sub.--1_P10 (SEQ ID NO:666), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence LMDQK (SEQ ID NO:948) corresponding to amino acids 499-503
of T46984_PEA.sub.--1_P10 (SEQ ID NO:666), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2521] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1.sub.--P10 (SEQ ID NO:666), comprising a
polypeptide being at least 70%, optionally at least about 80%,
preferably at least about 85%, more preferably at least about 90%
and most preferably at least about 95% homologous to the sequence
LMDQK (SEQ ID NO:948) in T46984_PEA.sub.--1_P10 (SEQ ID NO:666)
[2522] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2523] Variant protein T46984_PEA.sub.--1_P10 (SEQ ID NO:666) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 12, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P10 (SEQ ID
NO:666) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01034
TABLE 12 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->No 291 Q->P No 302 Q->R No 302 Q->L No 326
T->P No 330 D->N No 354 G->V Yes 425 T->P No 435
F->No 435 F->L No 449 F->No 460 K->*No 460 K->Q
No
[2524] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P10 (SEQ ID NO:666), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 13 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01035 TABLE 13
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2525] Variant protein T46984_PEA.sub.--1_P10 (SEQ ID NO:666) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T13 (SEQ
ID NO:596), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T13 (SEQ ID NO:596) is shown in bold; this
coding portion starts at position 316 and ends at position 1824.
The transcript also has the following SNPs as listed in Table 14
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P10 (SEQ ID NO:666) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01036 TABLE 14
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048 A->No 1048
A->G No 1068 A->C No 1076 G->A Yes 1187A->No
1187A->C No 1220A->G No 1220A->T No 1254T->G No 1291
A->C No 1293C->G No 1303G->A No 1376G->T Yes
1588A->C No 1618T->No 1618T->C No 1660 T->No
1693A->C No 1693A->T No 1845 T->No 1983 C->No 1983
C->A No 1990 C->No 1994 G->No 2179 C->A No 2199 T->C
Yes 2293 C->T No 2326 A->C No 2338 A->T No 2353 G->A
Yes 2359->A No 2368 A->G No 2393 T->G No 2407 T->A No
2407 T->G No 2507->C No 2516 T->No 2575 A->C No
[2526] Variant protein T46984_PEA.sub.--1_P11 (SEQ ID NO:667)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T14 (SEQ ID NO:597). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2527] Comparison report between T46984_PEA.sub.--1_P11 (SEQ ID
NO:667) and RIB2_HUMAN SEQ ID NO:663):
[2528] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P11 (SEQ ID NO:667), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL
FFQLVDVNTGAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDS
ASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEK
RPPTVVSNTFTALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWT
QLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR corresponding to amino acids
1-628 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds to
amino acids 1-628 of T46984_PEA.sub.--1_P11 (SEQ ID NO:667).
[2529] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: membrane. The protein localization is
believed to be membrane because although both signal-peptide
prediction programs agree that this protein has a signal peptide,
both trans-membrane region prediction programs predict that this
protein has a trans-membrane region downstream of this signal
peptide.
[2530] Variant protein T46984_PEA.sub.--1_P11 (SEQ ID NO:667) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 15, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P11 (SEQ ID
NO:667) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01037
TABLE 15 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->P No 291 Q->No 302 Q->L No 302 Q->R No 326
T->P No 330 D->N No 354 G->V Yes 425 T->P No 435
F->No 435 F->L No 449 F->No 460 K->Q No 460 K->*No
537 P->T No 537 P->No 539 T->No 540 V->No 602 T->N
No
[2531] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P11 (SEQ ID NO:667), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 16 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01038 TABLE 16
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2532] Variant protein T46984_PEA.sub.--1_P11 (SEQ ID NO:667) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T14 (SEQ
ID NO:597), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T14 (SEQ ID NO:597) is shown in bold; this
coding portion starts at position 316 and ends at position 2199.
The transcript also has the following SNPs as listed in Table 17
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P11 (SEQ ID NO:667) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01039 TABLE 17
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1187A->No 1187 A->C No
1220 A->G No 1220A->T No 1254 T->G No 1291 A->C No 1293
C->G No 1303 G->A No 1376 G->T Yes 1588 A->C No 1618
T->No 1618 T->C No 1660 T->No 1693A->C No 1693A->T
No 1924 C->No 1924 C->A No 1931 C->No 1935 G->No 2120
C->A No 2140 T->C Yes 2449 A->Yes 2537 C->T Yes 2614
C->T Yes 2699 C->T Yes 2857 G->A Yes 2879 A->G Yes 3078
A->G Yes 3354 G->A Yes
[2533] Variant protein T46984_PEA.sub.--1_P12 (SEQ ID NO:668)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T15 (SEQ ID NO:598). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2534] Comparison report between T46984_PEA.sub.--1_P12 (SEQ ID
NO:668) and RIB2_HUMAN (SEQ ID NO:663):
[2535] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P12 (SEQ ID NO:668), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMN corresponding to amino
acids 1-338 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds
to amino acids 1-338 of T46984_PEA.sub.--1_P12 (SEQ ID NO:668), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence SQDLH (SEQ ID NO:949) corresponding to amino acids
339-343 of T46984_PEA.sub.--1_P12 (SEQ ID NO:668), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2536] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P12 (SEQ ID NO:668), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence SQDLH (SEQ
ID NO:949) in T46984_PEA.sub.--1_P12 (SEQ ID NO:668).
[2537] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2538] Variant protein T46984_PEA.sub.--1_P12 (SEQ ID NO:668) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 18, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P12 (SEQ ID
NO:668) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01040
TABLE 18 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->No 291 Q->P No 302 Q->L No 302 Q->R No 326
T->P No 330 D->N No
[2539] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P12 (SEQ ID NO:668), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 19 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01041 TABLE 19
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2540] Variant protein T46984_PEA.sub.--1_P12 (SEQ ID NO:668) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T1 5
(SEQ ID NO:598), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
T46984_PEA.sub.--1_T15 (SEQ ID NO:598) is shown in bold; this
coding portion starts at position 316 and ends at position 1344.
The transcript also has the following SNPs as listed in Table 20
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P12 (SEQ ID NO:668) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01042 TABLE 20
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1187A->No 1187A->C No
1220A->G No 1220A->T No 1254 T->G No 1291 A->C No
1293C->G No 1303G->A No 1505A->C No 1535T->No
1535T->C No 1577T->No 1610 A->C No 1610 A->T No 1841
C->No 1841 C->A No 1848 C->No 1852 G->No 2037 C->A
No 2057 T->C Yes 2151 C->T No 2184 A->C No 2196 A->T No
2211 G->A Yes 2217->A No 2226 A->G No 2251 T->G No 2265
T->A No 2265 T->G No 2365->C No 2374 T->No 2433 A->C
No
[2541] Variant protein T46984_PEA.sub.--1_P21 (SEQ ID NO:669)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T27 (SEQ ID NO:601). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2542] Comparison report between T46984_PEA.sub.--1_P21 (SEQ ID
NO:669) and RIB2_HUMAN (SEQ ID NO:663):
[2543] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P21 (SEQ ID NO:669), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence M corresponding to amino acids 1-1 of
T46984_PEA.sub.--1_P21 (SEQ ID NO:669), and a second amino acid
sequence being at least 90% homologous to
KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAV
AALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA
RLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFES
LSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKL
EHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVEL
RVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNT
GAELTPHQTFVRLHNQKTGQEWFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLII
GDATLKNPILWNVADWIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTF
TALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKY
LAILGSVTFLAGNRMLAQQAVKRTAH corresponding to amino acids 70-631 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
2-563 of T46984_PEA.sub.--1_P21 (SEQ ID NO:669), wherein said first
amino acid sequence and second amino acid sequence are contiguous
and in a sequential order.
[2544] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: membrane. The protein localization is
believed to be membrane because both trans-membrane region
prediction programs predicted a trans-membrane region for this
protein. In addition both signal-peptide prediction programs
predict that this protein is a non-secreted protein.
[2545] Variant protein T46984_PEA.sub.--1_P21 (SEQ ID NO:669) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 21, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P21 (SEQ ID
NO:669) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01043
TABLE 21 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 31 G->R
Yes 67 F->No 69 L->No 122 R->No 177 N->No 177 N->D
No 183 E->D No 186 S->N Yes 223 Q->P No 223 Q->No 234
Q->L No 234 Q->R No 258 T->P No 262 D->N No 286 G->V
Yes 357 T->P No 367 F->L No 367 F->No 381 F->No 392
K->*No 392 K->Q No 469 P->No 469 P->T No 471 T->No
472 V->No 534 T->N No
[2546] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P21 (SEQ ID NO:669), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 22 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01044 TABLE 22
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
38
[2547] Variant protein T46984_PEA.sub.--1_P21 (SEQ ID NO:669) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T27 (SEQ ID NO:601) is shown in bold; this
coding portion starts at position 338 and ends at position 2026.
The transcript also has the following SNPs as listed in Table 23
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P21 (SEQ ID NO:669) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01045 TABLE 23
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 68 C->T Yes 194 A->G Yes 428
G->A Yes 536 T->No 542 C->No 662 C->T Yes 675->G No
703 C->No 715->G No 820 G->A No 866 A->No 866 A->G
No 886 A->C No 894 G->A Yes 1005A->No 1005 A->C No
1038A->G No 1038 A->T No 1072T->G No 1109 A->C No 1111
C->G No 1121 G->A No 1194G->T Yes 1406 A->C No 1436
T->No 1436 T->C No 1478 T->No 1511 A->C No 1511 A->T
No 1742 C->No 1742 C->A No 1749 C->No 1753 G->No 1938
C->A No 1958 T->C Yes 2052 C->T No 2085 A->C No 2097
A->T No 2112 G->A Yes 2118->A No 2127 A->G No 2152
T->G No 2166 T->A No 2166 T->G No 2266->C No 2275
T->No 2334 A->C No
[2548] Variant protein T46984_PEA.sub.--1_P27 (SEQ ID NO:670)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T34 (SEQ ID NO:603). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2549] Comparison report between T46984_PEA.sub.--1_P27 (SEQ ID
NO:670) and RIB2_HUMAN (SEQ ID NO:663):
[2550] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P27 (SEQ ID NO:670) comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN
RYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFA
corresponding to amino acids 1-415 of RIB2_HUMAN (SEQ ID NO:663),
which also corresponds to amino acids 1-415 of
T46984_PEA.sub.--1_P27 (SEQ ID NO:670), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO:950)
corresponding to amino acids 416-459 of T46984_PEA.sub.--1_P27 (SEQ
ID NO:670), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[2551] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P27 (SEQ ID NO:670), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
FGSGLVPMSPTSLLLLARLYFTWDMLLCWDSCMSTGLSSTCSRP (SEQ ID NO:950) in
T46984_PEA.sub.--1_P27 (SEQ ID NO:670).
[2552] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2553] Variant protein T46984_PEA.sub.--1_P27 (SEQ ID NO:670) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 24, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P27 (SEQ ID
NO:670) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01046
TABLE 24 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->No 291 Q->P No 302 Q->R No 302 Q->L No 326
T->P No 330 D->N No 354 G->V Yes 459 P->T No
[2554] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P27 (SEQ ID NO:670), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 25 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01047 TABLE 25
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2555] Variant protein T46984_PEA.sub.--1_P27 (SEQ ID NO:670) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T34 (SEQ
ID NO:603), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T34 (SEQ ID NO:603) is shown in bold; this
coding portion starts at position 316 and ends at position 1692.
The transcript also has the following SNPs as listed in Table 26
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P27 (SEQ ID NO:670) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01048 TABLE 26
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1187 A->No 1187 A->C No
1220 A->G No 1220 A->T No 1254 T->G No 1291 A->C No
1293 C->G No 1303 G->A No 1376 G->T Yes 1690 C->A No
1710 T->C Yes 1804 C->T No 1837 A->C No 1849A->T No
1864 G->A Yes 1870->A No 1879A->G No 1904T->G No 1918
T->A No 1918 T->G No 2018->C No 2027 T->No 2086 A->C
No
[2556] Variant protein T46984_PEA.sub.--1_P32 (SEQ ID NO:671)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T40 (SEQ ID NO:605). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2557] Comparison report between T46984_PEA.sub.--1_P32 (SEQ ID
NO:671) and RIB2_HUMAN (SEQ ID NO:663):
[2558] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDN RYIANTVE
corresponding to amino acids 1-364 of RIB2_HUMAN (SEQ ID NO:663),
which also corresponds to amino acids 1-364 of
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO:951) corresponding to
amino acids 365-397 of T46984_PEA.sub.--1_P32 (SEQ ID NO:671),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[2559] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
GQVRWLTPVIPALWEAKAGGSPEVRSSILAWPT (SEQ ID NO:951) in
T46984_PEA.sub.--1_P32 (SEQ ID NO:671).
[2560] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2561] Variant protein T46984_PEA.sub.--1_P32 (SEQ ID NO:671) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 27, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P32 (SEQ ID
NO:671) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01049
TABLE 27 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->No 291 Q->P No 302 Q->R No 302 Q->L No 326
T->P No 330 D->N No 354 G->V Yes
[2562] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P32 (SEQ ID NO:671), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 28 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01050 TABLE 28
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2563] Variant protein T46984_PEA.sub.--1_P32 (SEQ ID NO:671) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T40 (SEQ
ID NO:605), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T40 (SEQ ID NO:605) is shown in bold; this
coding portion starts at position 316 and ends at position 1506.
The transcript also has the following SNPs as listed in Table 29
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P32 (SEQ ID NO:671) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01051 TABLE 29
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1187 A->No 1187 A->C No
1220 A->G No 1220 A->T No 1254 T->G No 1291 A->C No
1293 C->G No 1303 G->A No 1376 G->T Yes
[2564] Variant protein T46984_PEA.sub.--1_P34 (SEQ ID NO:672)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T42 (SEQ ID NO:606). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2565] Comparison report between T46984_PEA.sub.--1_P34 (SEQ ID
NO:672) and RIB2_HUMAN (SEQ ID NO:663):
[2566] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P34 (SEQ ID NO:672), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQ
PLTQATVKLEHAKSVASRATVLQKTSFTPVG corresponding to amino acids 1-329
of RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino
acids 1-329 of T46984_PEA.sub.--1_P34 (SEQ ID NO:672).
[2567] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2568] Variant protein T46984_PEA.sub.--1_P34 (SEQ ID NO:672) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 30, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P34 (SEQ ID
NO:672) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01052
TABLE 30 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
291 Q->No 291 Q->P No 302 Q->L No 302 Q->R No 326
T->P No
[2569] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P34 (SEQ ID NO:672), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 31 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01053 TABLE 31
Glycosylation site(s) Position(s) on known Present in Position in
amino acid sequence variant protein? variant protein? 106 yes
106
[2570] Variant protein T46984_PEA.sub.--1_P34 (SEQ ID NO:672) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T42 (SEQ
ID NO:606), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T42 (SEQ ID NO:606) is shown in bold; this
coding portion starts at position 316 and ends at position 1302.
The transcript also has the following SNPs as listed in Table 32
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P34 (SEQ ID NO:672) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01054 TABLE 32
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 28 G->C No 173 G->C Yes 256
C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1187A->No 1187A->C No
1220A->G No 1220A->T No 1254T->G No 1291 A->C No 1293
C->G No 1324 T->C Yes 1489 G->A Yes
[2571] Variant protein T46984_PEA.sub.--1_P35 (SEQ ID NO:673)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T43 (SEQ ID NO:607). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2572] Comparison report between T46984_PEA.sub.--1_P35 (SEQ ID
NO:673) and RIB2_HUMAN (SEQ ID NO:663):
[2573] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P35 (SEQ ID NO:673), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSI
VEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNA
IFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI corresponding to
amino acids 1-287 of RIB2_HUMAN (SEQ ID NO:663), which also
corresponds to amino acids 1-287 of T46984_PEA.sub.--1_P35 (SEQ ID
NO:673), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ ID NO:952)
corresponding to amino acids 288-334 of T46984_PEA.sub.--1_P35 (SEQ
ID NO:673), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[2574] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P35 (SEQ ID NO:673), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01055 GCWPSRQSREQHISSRRKMEILKTECQEKESRTIHSMRRKMEKKNFI (SEQ
ID NO:952) in T46984_PEA.sub.--1_P35. (SEQ ID NO:673)
[2575] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2576] Variant protein T46984_PEA.sub.--1_P35 (SEQ ID NO:673) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 33, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P35 (SEQ ID
NO:673) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01056
TABLE 33 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No 190
R->No 245 N->No 245 N->D No 251 E->D No 254 S->N Yes
320 T->P No 324 M->L No 329 E->K Yes 334 1->V No
[2577] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P35 (SEQ ID NO:673), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 34 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01057 TABLE 34
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 106 yes
106
[2578] Variant protein T46984_PEA.sub.--1_P35 (SEQ ID NO:673) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T43 (SEQ
ID NO:607), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T43 (SEQ ID NO:607) is shown in bold; this
coding portion starts at position 316 and ends at position 1317.
The transcript also has the following SNPs as listed in Table 35
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein T46984_PEA.sub.--1_P35 (SEQ ID NO:673) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01058 TABLE 35
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 28 G->C No 173 G->C Yes
256 C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718T->No 724 C->No 844 C->T Yes 857->G No
885 C->No 897->G No 1002 G->A No 1048A->No 1048A->G
No 1068A->C No 1076 G->A Yes 1240 C->T No 1273A->C No
1285A->T No 1300G->A Yes 1306->A No 1315A->G No
1340T->G No 1354T->A No 1354T->G No 1454->C No
1463T->No 1522A->C No
[2579] Variant protein T46984_PEA.sub.--1_P38 (SEQ ID NO:674)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T47 (SEQ ID NO:609). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2580] Comparison report between T46984_PEA.sub.--1_P38 (SEQ ID
NO:674) and RIB2_HUMAN (SEQ ID NO:663):
[2581] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P38 (SEQ ID NO:674) comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEAL corresponding to amino acids 1-145 of
RIB2_HUMAN (SEQ ID NO:663), which also corresponds to amino acids
1-145 of T46984_PEA.sub.--1_P38 (SEQ ID NO:674), and a second amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MDPDWCQCLQLHFCS (SEQ ID NO:953) corresponding to amino
acids 146-160 of T46984_PEA.sub.--1_P38 (SEQ ID NO:674), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2582] 2. An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P38 (SEQ ID NO:674), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
MDPDWCQCLQLHFCS (SEQ ID NO:953) in T46984_PEA.sub.--1_P38 (SEQ ID
NO:674).
[2583] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2584] Variant protein T46984_PEA.sub.--1_P38 (SEQ ID NO:674) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 36, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P38 (SEQ ID
NO:674) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01059
TABLE 36 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No
[2585] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P38 (SEQ ID NO:674), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 37 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01060 TABLE 37
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 106 yes
106
[2586] Variant protein T46984_PEA.sub.--1_P38 (SEQ ID NO:674) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T47 (SEQ
ID NO:609), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T47 (SEQ ID NO:609) is shown in bold; this
coding portion starts at position 316 and ends at position 795. The
transcript also has the following SNPs as listed in Table 38 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984.sub.--PEA.sub.--1_P38 (SEQ ID NO:674) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01061 TABLE 38
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 28 G->C No 173 G->C Yes
256 C->T Yes 274 G->C Yes 325 C->No 389 C->G Yes 610
G->A Yes 718 T->No 724 C->No 879 C->A No 899 T->C
Yes 993 C->T No 1026 A->C No 1038 A->T No 1053 G->A Yes
1059->A No 1068 A->G No 1093 T->G No 1107 T->A No 1107
T->G No 1207->C No 1216 T->No 1275 A->C No
[2587] Variant protein T46984_PEA.sub.--1_P39 (SEQ ID NO:675)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T48 (SEQ ID NO:61 0). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2588] Comparison report between T46984_PEA.sub.--1_P39 (SEQ ID
NO:675) and RIB2_HUMAN (SEQ ID NO:663):
[2589] 1.An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P39 (SEQ ID NO:675), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSS
VTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLA corresponding to amino
acids 1-160 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds
to amino acids 1-160 of T46984_PEA.sub.--1_P39 (SEQ ID NO:675).
[2590] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2591] Variant protein T46984_PEA.sub.--1_P39 (SEQ ID NO:675) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 39, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P39 (SEQ ID
NO:675) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01062
TABLE 39 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 P->No
25 P->R Yes 99 G->R Yes 135 F->No 137 L->No
[2592] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P39 (SEQ ID NO:675), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 40 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01063 TABLE 40
Glycosylation site(s) Position(s) on known amino Present in acid
sequence variant protein? Position in variant protein? 106 yes
106
[2593] Variant protein T46984_PEA.sub.--1_P39 (SEQ ID NO:675) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T48 (SEQ
ID NO:610), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T48 (SEQ ID NO:610) is shown in bold; this
coding portion starts at position 316 and ends at position 795. The
transcript also has the following SNPs as listed in Table 41 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984_PEA.sub.--1_P39 (SEQ ID NO:675) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01064 TABLE 41 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 28 G->C No 173 G->C Yes 256 C->T Yes
274 G->C Yes 325 C->No 389 C->G Yes 610 G->A Yes 718
T->No 724 C->No 848 G->T Yes 879 C->G Yes 1008A->G
Yes 1397 A->G Yes
[2594] Variant protein T46984_PEA.sub.--1_P45 (SEQ ID NO:676)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T32 (SEQ ID NO:602). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2595] Comparison report between T46984_PEA.sub.--1_P45 (SEQ ID
NO:676) and RIB2_HUMAN (SEQ. ID NO:663):
[2596] 1.An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P45 (SEQ ID NO:676), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCE corresponding to amino
acids 1-101 of RIB2_HUMAN (SEQ ID NO:663), which also corresponds
to amino acids 1-101 of T46984_PEA.sub.--1_P45 (SEQ ID NO:676), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence NSPGSADSIPPVPAG (SEQ ID NO:954) corresponding to amino
acids 102-116 of T46984_PEA.sub.--1_P45 (SEQ ID NO:676), wherein
said first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2597] 2.An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P45 (SEQ ID NO:676), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) in T46984_PEA.sub.--1_P45 (SEQ ID
NO:676).
[2598] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2599] Variant protein T46984_PEA.sub.--1_P45 (SEQ ID NO:676) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 42, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P45 (SEQ ID
NO:676) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01065
TABLE 42 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 P->No
25 P->R Yes 99 G->R Yes
[2600] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P45 (SEQ ID NO:676), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 43 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01066 TABLE 43
Glycosylation site(s) Position(s) on known Present in amino acid
sequence variant protein? 106 no
[2601] Variant protein T46984_PEA.sub.--1_P45 (SEQ ID NO:676) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T32 (SEQ ID NO:602) is shown in bold; this
coding portion starts at position 316 and ends at position 663. The
transcript also has the following SNPs as listed in Table 44 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984_PEA.sub.--1_P45 (SEQ ID NO:676) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01067 TABLE 44 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 28 G->C No 173 G->C Yes 256 C->T
Yes 274 G->C Yes 325 C->No 389 C->G Yes 610 G->A Yes
668 C->T Yes 681->G No 709 C->No 721->G No 826 G->A
No 872 A->No 872 A->G No 892 A->C No 900 G->A Yes 1011
A->No 1011 A->C No 1044 A->G No 1044 A->T No 1078
T->G No 1115A->C No 1117 C->G No 1127 G->A No 1200
G->T Yes 1412 A->C No 1442 T->No 1442 T->C No 1484
T->No 1517 A->C No 1517 A->T No 1748 C->No 1748 C->A
No 1755 C->No 1759 G->No 1944 C->A No 1964 T->C Yes
2058 C->T No 2091 A->C No 2103A->T No 2118 G->A Yes
2124->A No 2133A->G No 2158 T->G No 2172 T->A No 2172
T->G No 2272->C No 2281 T->No 2340 A->C No
[2602] Variant protein T46984_PEA.sub.--1_P46 (SEQ ID NO:677)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T46984_PEA.sub.--1_T35 (SEQ ID NO:604). An alignment is given to
the known protein (Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2603] Comparison report between T46984_PEA.sub.--1_P46 (SEQ ID
NO:677) and RIB2_HUMAN (SEQ. ID NO:663):
[2604] 1. An isolated chimeric polypeptide encoding for
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), comprising a first amino
acid sequence being at least 90% homologous to
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSL
GAQVPDAK corresponding to amino acids 1-69 of RIB2_HUMAN (SEQ ID
NO:663), which also corresponds to amino acids 1-69 of
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) corresponding to amino acids 70-84
of T46984_PEA.sub.--1_P46 (SEQ ID NO:677), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2605] 2.An isolated polypeptide encoding for a tail of
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
NSPGSADSIPPVPAG (SEQ ID NO:954) in T46984_PEA.sub.--1_P46 (SEQ ID
NO:677).
[2606] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2607] Variant protein T46984_PEA.sub.--1_P46 (SEQ ID NO:677) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 45, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T46984_PEA.sub.--1_P46 (SEQ ID
NO:677) sequence provides support for the deduced sequence of this
variant protein according to the present invention) TABLE-US-01068
TABLE 45 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 P->No
25 P->R Yes
[2608] The glycosylation sites of variant protein
T46984_PEA.sub.--1_P46 (SEQ ID NO:677), as compared to the known
protein Dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 63 kDa subunit precursor (SEQ ID NO:663), are
described in Table 46 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01069 TABLE 46
Glycosylation site(s) Position(s) on known Present in amino acid
sequence variant protein? 106 no
[2609] Variant protein T46984_PEA.sub.--1_P46 (SEQ ID NO:677) is
encoded by the following transcript(s): T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) is shown in bold; this
coding portion starts at position 316 and ends at position 567. The
transcript also has the following SNPs as listed in Table 47 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T46984_PEA.sub.--1_P46 (SEQ ID NO:677) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01070 TABLE 47 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 28 G->C No 173 G->C Yes 256 C->T
Yes 274 G->C Yes 325 C->No 389 C->G Yes 572 C->T Yes
585->G No 613 C->No 625->G No 730 G->A No 776 A->No
776 A->G No 796A->C No 804 G->A Yes 915A->No 915A->C
No 948 A->G No 948 A->T No 982T->G No 1019 A->C No 1021
C->G No 1031 G->A No 1104G->T Yes 1316 A->C No 1346
T->No 1346 T->C No 1388 T->No 1421 A->C No 1421 A->T
No 1652 C->No 1652 C->A No 1659 C->No 1663 G->No 1848
C->A No 1868T->C Yes 1962 C->T No 1995A->C No 2007
A->T No 2022 G->A Yes 2028->A No 2037 A->G No 2062
T->G No 2076 T->A No 2076 T->G No 2176->C No 2185
T->No 2244 A->C No
[2610] As noted above, cluster T46984 features 49 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[2611] Segment cluster T46984_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:614) according to the present invention is supported by 240
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T40 (SEQ
ID NO:605), T46984_PEA.sub.--1_T42 (SEQ ID NO:606),
T46984_PEA.sub.--1_T43 (SEQ ID NO:607), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609) and T46984_PEA.sub.--1_T48 (SEQ ID NO:610). Table 48
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01071 TABLE 48 Segment location on
transcripts Segment Segment starting ending Transcript name
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1 328
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1 328 T46984_PEA.sub.--1_T12
(SEQ ID 1 328 NO: 595) T46984_PEA.sub.--1_T13 (SEQ ID 1 328 NO:
596) T46984_PEA.sub.--1_T14 (SEQ ID 1 328 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1 328 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1 328 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1 328 NO: 600)
T46984_PEA.sub.--1_T32 (SEQ ID 1 328 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1 328 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1 328 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1 328 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 1 328 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 1 328 NO: 607)
T46984_PEA.sub.--1_T47 (SEQ ID 1 328 NO: 609)
T46984_PEA.sub.--1_T48 (SEQ ID 1 328 NO: 610)
[2612] Segment cluster T46984_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:615) according to the present invention is supported by 321
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T40 (SEQ
ID NO:605), T46984_PEA.sub.--1_T42 (SEQ ID NO:606),
T46984_PEA.sub.--1_T43 (SEQ ID NO:607), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609) and T46984_PEA.sub.--1_T48 (SEQ ID NO:610). Table 49
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01072 TABLE 49 Segment location on
transcripts Segment Segment starting ending Transcript name
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 329 522
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 329 522
T46984_PEA.sub.--1_T12 (SEQ ID 329 522 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 329 522 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 329 522 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 329 522 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 329 522 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 329 522 NO: 600)
T46984_PEA.sub.--1_T32 (SEQ ID 329 522 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 329 522 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 329 522 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 329 522 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 329 522 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 329 522 NO: 607)
T46984_PEA.sub.--1_T47 (SEQ ID 329 522 NO: 609)
T46984_PEA.sub.--1_T48 (SEQ ID 329 522 NO: 610)
[2613] Segment cluster T46984_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:616) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T27 (SEQ ID NO:601). Table 50
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01 073 TABLE 50 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T27 (SEQ ID 1 340 NO: 601)
[2614] Segment cluster T46984_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:617) according to the present invention is supported by 262
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T40 (SEQ ID NO:605), T46984_PEA.sub.--1_T42 (SEQ
ID NO:606), T46984_PEA.sub.--1_T43 (SEQ ID NO:607),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609) and T46984_PEA.sub.--1_T48
(SEQ ID NO:610). Table 51 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01074 TABLE
51 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T2 (SEQ ID NO:
593) 619 751 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 619 751
T46984_PEA.sub.--1_T12 (SEQ ID 619 751 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 619 751 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 619 751 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 619 751 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 619 751 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 619 751 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 437 569 NO: 601)
T46984_PEA.sub.--1_T34 (SEQ ID 619 751 NO: 603)
T46984_PEA.sub.--1_T40 (SEQ ID 619 751 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 619 751 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 619 751 NO: 607)
T46984_PEA.sub.--1_T47 (SEQ ID 619 751 NO: 609)
T46984_PEA.sub.--1_T48 (SEQ ID 619 751 NO: 610)
[2615] Segment cluster T46984_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:618) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T48 (SEQ ID NO:610). Table 52
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01 075 TABLE 52 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T48 (SEQ ID 795 1718 NO:
610)
[2616] Segment cluster T46984_PEA.sub.--1_node.sub.--25 (SEQ ID
NO:619) according to the present invention is supported by 257
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605),
T46984_PEA.sub.--1_T42 (SEQ ID NO:606) and T46984_PEA.sub.--1_T43
(SEQ ID NO:607). Table 53 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01076 TABLE
53 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T2 (SEQ ID NO:
593) 1006 1171 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1006 1171
T46984_PEA.sub.--1_T12 (SEQ ID 1006 1171 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1006 1171 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1006 1171 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1006 1171 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1006 1171 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1006 1171 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 824 989 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 830 995 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1006 1171 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 734 899 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1006 1171 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 1006 1171 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 1006 1171 NO: 607)
[2617] Segment cluster T46984_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:620) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T42 (SEQ ID NO:606). Table 54
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01077 TABLE 54 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T42 (SEQ ID 1302 1501 NO:
606)
[2618] Segment cluster T46984_PEA.sub.--1_node.sub.--34 (SEQ ID
NO:621) according to the present invention is supported by 4
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T40 (SEQ ID NO:605). Table 55
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01078 TABLE 55 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T40 (SEQ ID 1408 1717 NO:
605)
[2619] Segment cluster T46984_PEA.sub.--1_node.sub.--46 (SEQ ID
NO:622) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T46 (SEQ ID NO:608). Table 56
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01079 TABLE 56 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T46 (SEQ ID 1 306 NO: 608)
[2620] Segment cluster T46984_PEA.sub.--1_node.sub.--47 (SEQ ID
NO:623) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T3 (SEQ ID NO:594),
T46984_PEA.sub.--1_T19 (SEQ ID NO:599) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 57 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01080 TABLE
57 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T3 (SEQ ID NO:
594) 1615 2242 T46984_PEA.sub.--1_T19 (SEQ ID 1615 2242 NO: 599)
T46984_PEA.sub.--1_T46 (SEQ ID 307 934 NO: 608)
[2621] Segment cluster T46984_PEA.sub.--1_node.sub.--52 (SEQ ID
NO:624) according to the present invention is supported by 29
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T19 (SEQ ID NO:599) and T46984_PEA.sub.--1_T23
(SEQ ID NO:600). Table 58 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01081 TABLE
58 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T2 (SEQ ID NO:
593) 1838 2904 T46984_PEA.sub.--1_T19 (SEQ ID 2466 3532 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1838 2904 NO: 600)
[2622] Segment cluster T46984_PEA.sub.--1_node.sub.--65 (SEQ ID
NO:625) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T51 (SEQ ID NO:611). Table 59
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01082 TABLE 59 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T51 (SEQ ID 1 348 NO: 611)
[2623] Segment cluster T46984_PEA.sub.--1_node.sub.--69 (SEQ ID
NO:626) according to the present invention is supported by 8
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 60 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01083 TABLE 60 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T46984_PEA.sub.--1_T52 (SEQ ID 1 927 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1 927 NO: 613)
[2624] Segment cluster T46984_PEA.sub.--1_node.sub.--75 (SEQ ID
NO:627) according to the present invention is supported by 5
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T14 (SEQ ID NO:597). Table 61
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01084 TABLE 61 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T14 (SEQ ID 2199 3529 NO:
597)
[2625] Segment cluster T46984_PEA.sub.--1_node.sub.--86 (SEQ ID
NO:628) according to the present invention is supported by 314
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T15 (SEQ ID NO:598), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T43 (SEQ
ID NO:607), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 62 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01 085 TABLE 62 Segment location on
transcripts Segment Segment starting ending Transcript name
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 3492 3750
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 2886 3144
T46984_PEA.sub.--1_T12 (SEQ ID 2286 2544 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2317 2575 NO: 596)
T46984_PEA.sub.--1_T15 (SEQ ID 2175 2433 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4120 4378 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3396 3654 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2076 2334 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2082 2340 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1828 2086 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1986 2244 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1264 1522 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1578 1836 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 1017 1275 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 614 872 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1117 1375 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1117 1602 NO: 613)
[2626] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2627] Segment cluster T46984_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:629) according to the present invention is supported by 304
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T40 (SEQ
ID NO:605), T46984_PEA.sub.--1_T42 (SEQ ID NO:606),
T46984_PEA.sub.--1_T43 (SEQ ID NO:607), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609) and T46984_PEA.sub.--1_T48 (SEQ ID NO:610). Table 63
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01086 TABLE 63 Segment location on
transcripts Segment Segment starting ending Transcript name
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 523 618
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 523 618
T46984_PEA.sub.--1_T12 (SEQ ID 523 618 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 523 618 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 523 618 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 523 618 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 523 618 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 523 618 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 341 436 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 523 618 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 523 618 NO: 603)
T46984_PEA.sub.--1_T40 (SEQ ID 523 618 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 523 618 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 523 618 NO: 607)
T46984_PEA.sub.--1_T47 (SEQ ID 523 618 NO: 609)
T46984_PEA.sub.--1_T48 (SEQ ID 523 618 NO: 610)
[2628] Segment cluster T46984_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:630) according to the present invention is supported by 232
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T40 (SEQ ID NO:605), T46984_PEA.sub.--1_T42 (SEQ
ID NO:606), T46984_PEA.sub.--1_T43 (SEQ ID NO:607) and
T46984_PEA.sub.--1_T48 (SEQ ID NO:610). Table 64 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01087 TABLE 64 Segment location on transcripts
Segment Segment starting ending Transcript name position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 752 794
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 752 794
T46984_PEA.sub.--1_T12 (SEQ ID 752 794 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 752 794 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 752 794 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 752 794 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 752 794 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 752 794 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 570 612 NO: 601)
T46984_PEA.sub.--1_T34 (SEQ ID 752 794 NO: 603)
T46984_PEA.sub.--1_T40 (SEQ ID 752 794 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 752 794 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 752 794 NO: 607)
T46984_PEA.sub.--1_T48 (SEQ ID 752 794 NO: 610)
[2629] Segment cluster T46984_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:631) according to the present invention is supported by 237
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605),
T46984_PEA.sub.--1_T42 (SEQ ID NO:606) and T46984_PEA.sub.--1_T43
(SEQ ID NO:607). Table 65 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01 088 TABLE
65 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T2 (SEQ ID NO:
593) 795 870 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 795 870
T46984_PEA.sub.--1_T12 (SEQ ID 795 870 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 795 870 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 795 870 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 795 870 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 795 870 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 795 870 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 613 688 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 619 694 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 795 870 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 523 598 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 795 870 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 795 870 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 795 870 NO: 607)
[2630] Segment cluster T46984_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:632) according to the present invention is supported by 242
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605),
T46984_PEA.sub.--1_T42 (SEQ ID NO:606) and T46984_PEA.sub.--1_T43
(SEQ ID NO:607). Table 66 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01089 TABLE
66 Segment location on transcripts Segment Segment starting ending
Transcript name position position T46984_PEA.sub.--1_T2 (SEQ ID NO:
593) 871 975 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 871 975
T46984_PEA.sub.--1_T12 (SEQ ID 871 975 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 871 975 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 871 975 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 871 975 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 871 975 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 871 975 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 689 793 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 695 799 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 871 975 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 599 703 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 871 975 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 871 975 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 871 975 NO: 607)
[2631] Segment cluster T46984_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:633) according to the present invention is supported by 205
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605),
T46984_PEA.sub.--1_T42 (SEQ ID NO:606) and T46984_PEA.sub.--1_T43
(SEQ ID NO:607). Table 67 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01090 TABLE
67 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 976 1005 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
976 1005 T46984_PEA.sub.--1_T12 (SEQ ID 976 1005 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 976 1005 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 976 1005 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 976 1005 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 976 1005 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 976 1005 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 794 823 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 800 829 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 976 1005 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 704 733 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 976 1005 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 976 1005 NO: 606)
T46984_PEA.sub.--1_T43 (SEQ ID 976 1005 NO: 607)
[2632] Segment cluster T46984_PEA.sub.--1_node.sub.--26 (SEQ ID
NO:634) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605) and
T46984_PEA.sub.--1_T42 (SEQ ID NO:606). Table 68 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01091 TABLE 68 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1172 1182
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1172 1182
T46984_PEA.sub.--1_T12 (SEQ ID 1172 1182 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1172 1182 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1172 1182 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1172 1182 NO: 598)
T46984_PEA.sub.--1 T19 (SEQ ID 1172 1182 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1172 1182 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 990 1000 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 996 1006 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1172 1182 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 900 910 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1172 1182 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 1172 1182 NO: 606)
[2633] Segment cluster T46984_PEA.sub.--1_node.sub.--28 (SEQ ID
NO:635) according to the present invention is supported by 242
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T40 (SEQ ID NO:605) and
T46984_PEA.sub.--1_T42 (SEQ ID NO:606). Table 69 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01092 TABLE 69 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1183 1301
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1183 1301
T46984_PEA.sub.--1_T12 (SEQ ID 1183 1301 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1183 1301 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1183 1301 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1183 1301 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1183 1301 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1183 1301 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1001 1119 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1007 1125 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1183 1301 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 911 1029 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1183 1301 NO: 605)
T46984_PEA.sub.--1_T42 (SEQ ID 1183 1301 NO: 606)
[2634] Segment cluster T46984_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:636) according to the present invention is supported by 207
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604) and T46984_PEA.sub.--1_T40 (SEQ ID NO:605). Table 70
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01 093 TABLE 70 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1302 1329
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1302 1329
T46984_PEA.sub.--1_T12 (SEQ ID 1302 1329 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1302 1329 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1302 1329 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1302 1329 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1302 1329 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1302 1329 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1120 1147 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1126 1153 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1302 1329 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1030 1057 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1302 1329 NO: 605)
[2635] Segment cluster T46984_PEA.sub.--1_node.sub.--32 (SEQ ID
NO:637) according to the present invention is supported by 226
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T40
(SEQ ID NO:605). Table 71 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01094 TABLE
71 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1330 1407 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
1330 1407 T46984_PEA.sub.--1_T12 (SEQ ID 1330 1407 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1330 1407 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1330 1407 NO: 597)
T46984_PEA.sub.--1_T19 (SEQ ID 1330 1407 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1330 1407 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1148 1225 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1154 1231 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1330 1407 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1058 1135 NO: 604)
T46984_PEA.sub.--1_T40 (SEQ ID 1330 1407 NO: 605)
[2636] Segment cluster T46984_PEA.sub.--1_node.sub.--38 (SEQ ID
NO:638) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603) and
T46984_PEA.sub.--1_T35 (SEQ ID NO:604). Table 72 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01095 TABLE 72 Segment location on transcripts
Segment Segment starting ending Transcript name position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1408 1412
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1408 1412
T46984_PEA.sub.--1_T12 (SEQ ID 1408 1412 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1408 1412 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1408 1412 NO: 597)
T46984_PEA.sub.--1_T19 (SEQ ID 1408 1412 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1408 1412 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1226 1230 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1232 1236 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1408 1412 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1136 1140 NO: 604)
[2637] Segment cluster T46984_PEA.sub.--1_node.sub.--39 (SEQ ID
NO:639) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603) and T46984_PEA.sub.--1_T35
(SEQ ID NO:604). Table 73 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01096 TABLE
73 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1413 1435 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
1413 1435T46984_PEA.sub.--1_T12 (SEQ ID 1413 1435 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1413 1435 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1413 1435 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1330 1352 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1413 1435 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1413 1435 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1231 1253 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1237 1259 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1413 1435 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1141 1163 NO: 604)
[2638] Segment cluster T46984_PEA.sub.--1_node.sub.--40 (SEQ ID
NO:640) according to the present invention is supported by 227
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603) and T46984_PEA.sub.--1_T35
(SEQ ID NO:604). Table 74 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01097 TABLE
74 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1436 1499 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
1436 1499 T46984_PEA.sub.--1_T12 (SEQ ID 1436 1499 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1436 1499 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1436 1499 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1353 1416 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1436 1499 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1436 1499 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1254 1317 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1260 1323 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1436 1499 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1164 1227 NO: 604)
[2639] Segment cluster T46984_PEA.sub.--1_node.sub.--42 (SEQ ID
NO:641) according to the present invention is supported by 239
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603) and T46984_PEA.sub.--1_T35
(SEQ ID NO:604). Table 75 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01098 TABLE
75 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1500 1562 T46984 PEA.sub.--1_T3 (SEQ ID NO: 594)
1500 1562 T46984_PEA.sub.--1_T12 (SEQ ID 1500 1562 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1500 1562 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1500 1562 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1417 1479 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1500 1562 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1500 1562 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1318 1380 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1324 1386 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1500 1562 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1228 1290 NO: 604)
[2640] Segment cluster T46984_PEA.sub.--1_node.sub.--43 (SEQ ID
NO:642) according to the present invention is supported by 235
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602) and
T46984_PEA.sub.--1_T35 (SEQ ID NO:604). Table 76 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01099 TABLE 76 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1563 1614
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 1563 1614
T46984_PEA.sub.--1_T12 (SEQ ID 1563 1614 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1563 1614 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1563 1614 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1480 1531 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 1563 1614 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1563 1614 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1381 1432 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1387 1438 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1291 1342 NO: 604)
[2641] Segment cluster T46984_PEA.sub.--1_node.sub.--48 (SEQ ID
NO:643) according to the present invention is supported by 282
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 77 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01100 TABLE
77 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1615 1715 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2243 2343 T46984_PEA.sub.--1_T12 (SEQ ID 1615 1715 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1615 1715 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1615 1715 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1532 1632 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 2243 2343 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1615 1715 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1433 1533 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1439 1539 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1343 1443 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 935 1035 NO: 608)
[2642] Segment cluster T46984_PEA.sub.--1_node.sub.--49 (SEQ ID
NO:644) according to the present invention is supported by 262
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 78 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01101 TABLE
78 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1716 1757 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2344 2385 T46984_PEA.sub.--1_T12 (SEQ ID 1716 1757 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1716 1757 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1716 1757 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1633 1674 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 2344 2385 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1716 1757 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1534 1575 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1540 1581 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1444 1485 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1036 1077 NO: 608)
[2643] Segment cluster T46984_PEA.sub.--1_node.sub.--50 (SEQ ID
NO:645) according to the present invention is supported by 277
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 79 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01102 TABLE
79 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 1758 1809 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2386 2437 T46984_PEA.sub.--1_T12 (SEQ ID 1758 1809 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1758 1809 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1758 1809 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1675 1726 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 2386 2437 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1758 1809 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1576 1627 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1582 1633 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1486 1537 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1078 1129 NO: 608)
[2644] Segment cluster T46984_PEA.sub.--1_node.sub.--51 (SEQ ID
NO:646) according to the present invention is supported by 6
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T12 (SEQ ID NO:595), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599) and T46984_PEA.sub.--1_T23 (SEQ ID NO:600). Table 80
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01103 TABLE 80 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 1810 1837
T46984_PEA.sub.--1_T12 (SEQ ID 1810 1837 NO: 595)
T46984_PEA.sub.--1_T19 (SEQ ID 2438 2465 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 1810 1837 NO: 600)
[2645] Segment cluster T46984_PEA.sub.--1_node.sub.--53 (SEQ ID
NO:647) according to the present invention is supported by 16
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T13 (SEQ ID NO:596), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599) and T46984_PEA.sub.--1_T23 (SEQ ID NO:600). Table 81
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01104 TABLE 81 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 2905 2963
T46984_PEA.sub.--1_T13 (SEQ ID 1810 1868 NO: 596)
T46984_PEA.sub.--1_T19 (SEQ ID 3533 3591 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 2905 2963 NO: 600)
[2646] Segment cluster T46984_PEA.sub.--1_node.sub.--54 (SEQ ID
NO:648) according to the present invention is supported by 18
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T19 (SEQ ID NO:599) and T46984_PEA.sub.--1_T23
(SEQ ID NO:600). Table 82 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01105 TABLE
82 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 2964 3043 T46984_PEA.sub.--1_T19 (SEQ ID 3592 3671
NO: 599) T46984_PEA.sub.--1_T23 (SEQ ID 2964 3043 NO: 600)
[2647] Segment cluster T46984_PEA.sub.--1_node.sub.--55 (SEQ ID
NO:649) according to the present invention is supported by 335
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1.sub.--T12
(SEQ ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 83 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01106 TABLE
83 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 3044 3110 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2438 2504 T46984_PEA.sub.--1_T12 (SEQ ID 1838 1904 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1869 1935 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1810 1876 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1727 1793 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3672 3738 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3044 3110 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1628 1694 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1634 1700 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1538 1604 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1130 1196 NO: 608)
[2648] Segment cluster T46984_PEA.sub.--1_node.sub.--57 (SEQ ID
NO:650) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and T46984_PEA.sub.--1_T46
(SEQ ID NO:608). Table 84 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01107 TABLE
84 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 3111 3130 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2505 2524 T46984_PEA.sub.--1_T12 (SEQ ID 1905 1924 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1936 1955 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1877 1896 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1794 1813 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3739 3758 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3111 3130 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1695 1714 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1701 1720 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1605 1624 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1197 1216 NO: 608)
[2649] Segment cluster T46984_PEA.sub.--1_node.sub.--60 (SEQ ID
NO:651) according to the present invention is supported by 326
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and
T46984_PEA.sub.--1_T46 (SEQ ID NO:608). Table 85 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01108 TABLE 85 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 3131 3165
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 2525 2559
T46984_PEA.sub.--1_T12 (SEQ ID 1925 1959 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1956 1990 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1897 1931 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1814 1848 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3759 3793 NO: 599)
T46984_PEA.sub.--1_T27 (SEQ ID 1715 1749 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1721 1755 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1625 1659 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1217 1251 NO: 608)
[2650] Segment cluster T46984_PEA.sub.--1_node.sub.--62 (SEQ ID
NO:652) according to the present invention is supported by 335
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T35 (SEQ ID NO:604) and
T46984_PEA.sub.--1_T46 (SEQ ID NO:608). Table 86 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01109 TABLE 86 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 3166 3226
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 2560 2620
T46984_PEA.sub.--1_T12 (SEQ ID 1960 2020 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 1991 2051 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1932 1992 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1849 1909 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3794 3854 NO: 599)
T46984_PEA.sub.--1_T27 (SEQ ID 1750 1810 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1756 1816 NO: 602)
T46984_PEA.sub.--1_T35 (SEQ ID 1660 1720 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1252 1312 NO: 608)
[2651] Segment cluster T46984_PEA.sub.--1_node.sub.--66 (SEQ ID
NO:653) according to the present invention is supported by 336
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609) and T46984_PEA.sub.--1_T51
(SEQ ID NO:611). Table 87 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01110 TABLE
87 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 3227 3261 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2621 2655 T46984_PEA.sub.--1_T12 (SEQ ID 2021 2055 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2052 2086 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 1993 2027 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1910 1944 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3855 3889 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3131 3165 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1811 1845 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1817 1851 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1563 1597 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1721 1755 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1313 1347 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 752 786 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 349 383 NO: 611)
[2652] Segment cluster T46984_PEA.sub.--1_node.sub.--67 (SEQ ID
NO:654) according to the present invention is supported by 323
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609) and T46984_PEA.sub.--1_T51
(SEQ ID NO:611). Table 88 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01111 TABLE
88 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 3262 3302 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2656 2696 T46984_PEA.sub.--1_T12 (SEQ ID 2056 2096 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2087 2127 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 2028 2068 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1945 1985 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3890 3930 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3166 3206 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1846 1886 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1852 1892 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1598 1638 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1756 1796 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1348 1388 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 787 827 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 384 424 NO: 611)
[2653] Segment cluster T46984_PEA.sub.--1_node.sub.--70 (SEQ ID
NO:655) according to the present invention is supported by 337
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 89 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01112 TABLE 89 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 3303 3377
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 2697 2771
T46984_PEA.sub.--1_T12 (SEQ ID 2097 2171 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2128 2202 NO:
596)-T46984_PEA.sub.--1_T14 (SEQ ID 2069 2143 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 1986 2060 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 3931 4005 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3207 3281 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1887 1961 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1893 1967 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1639 1713 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1797 1871 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1389 1463 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 828 902 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 425 499 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 928 1002 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 928 1002 NO: 613)
[2654] Segment cluster T46984_PEA.sub.--1_node.sub.--71 (SEQ ID
NO:656) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 90 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01113 TABLE 90 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 593) 3378 3399
T46984_PEA.sub.--1_T3 (SEQ ID NO: 594) 2772 2793
T46984_PEA.sub.--1_T12 (SEQ ID 2172 2193 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2203 2224 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 2144 2165 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 2061 2082 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4006 4027 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3282 3303 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1962 1983 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1968 1989 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1714 1735 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1872 1893 NO: 604)
T46984_PEA.sub.--1_T46 (SEQ ID 1464 1485 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 903 924 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 500 521 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1003 1024 NO: 612)
T46984_PEA.sub.--1 T54 (SEQ ID 1003 1024 NO: 613)
[2655] Segment cluster T46984_PEA.sub.--1_node.sub.--72 (SEQ ID
NO:657) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T43 (SEQ ID NO:607),
T46984_PEA.sub.--1_T46 (SEQ ID NO:608), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609), T46984_PEA.sub.--1_T51 (SEQ ID NO:611),
T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and T46984_PEA.sub.--1_T54
(SEQ ID NO:613). Table 91 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01114 TABLE
91 Segment location on transcripts Segment Segment ending
Transcript name starting position position T46984_PEA.sub.--1_T2
(SEQ ID NO: 593) 3400 3421 T46984_PEA.sub.--1_T3 (SEQ ID NO: 594)
2794 2815 T46984_PEA.sub.--1_T12 (SEQ ID 2194 2215 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2225 2246 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 2166 2187 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 2083 2104 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4028 4049 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3304 3325 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 1984 2005 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 1990 2011 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1736 1757 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1894 1915 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1172 1193 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1486 1507 NO: 608)
T46984_PEA.sub.--1 T47 (SEQ ID 925 946 NO: 609) T46984_PEA.sub.--1
T51 (SEQ ID 522 543 NO: 611) T46984_PEA.sub.--1_T52 (SEQ ID 1025
1046 NO: 612) T46984_PEA.sub.--1_T54 (SEQ ID 1025 1046 NO: 613)
[2656] Segment cluster T46984_PEA.sub.--1_node.sub.--73 (SEQ ID
NO:658) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T43 (SEQ ID NO:607),
T46984_PEA.sub.--1_T46 (SEQ ID NO:608), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609), T46984_PEA.sub.--1_T51 (SEQ ID NO:611),
T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and T46984_PEA.sub.--1_T54
(SEQ ID NO:613). Table 92 below describes the starting and ending
position of this segment on each transcript. TABLE-US-0111 5 TABLE
92 Segment location on transcripts Segment Segment Transcript name
starting position ending position T46984_PEA.sub.--1_T2 3422 3428
(SEQ ID NO: 593) T46984_PEA.sub.--1_T3 2816 2822 (SEQ ID NO: 594)
T46984_PEA.sub.--1_T12 (SEQ ID 2216 2222 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2247 2253 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 2188 2194 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 2105 2111 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4050 4056 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3326 3332 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2006 2012 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2012 2018 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1758 1764 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1916 1922 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1194 1200 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1508 1514 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 947 953 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 544 550 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1047 1053 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1047 1053 NO: 613)
[2657] Segment cluster T46984_PEA.sub.--1_node.sub.--74 (SEQ ID
NO:659) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T14 (SEQ ID NO:597), T46984_PEA.sub.--1_T15 (SEQ
ID NO:598), T46984_PEA.sub.--1_T19 (SEQ ID NO:599),
T46984_PEA.sub.--1_T23 (SEQ ID NO:600), T46984_PEA.sub.--1_T27 (SEQ
ID NO:601), T46984_PEA.sub.--1_T32 (SEQ ID NO:602),
T46984_PEA.sub.--1_T34 (SEQ ID NO:603), T46984_PEA.sub.--1_T35 (SEQ
ID NO:604), T46984_PEA.sub.--1_T43 (SEQ ID NO:607),
T46984_PEA.sub.--1_T46 (SEQ ID NO:608), T46984_PEA.sub.--1_T47 (SEQ
ID NO:609), T46984_PEA.sub.--1_T51 (SEQ ID NO:611),
T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and T46984_PEA.sub.--1_T54
(SEQ ID NO:613). Table 93 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01116 TABLE
93 Segment location on transcripts Segment Segment Transcript name
starting position ending position T46984_PEA.sub.--1_T2 3429 3432
(SEQ ID NO: 593) T46984_PEA.sub.--1_T3 2823 2826 (SEQ ID NO: 594)
T46984_PEA.sub.--1_T12 (SEQ ID 2223 2226 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2254 2257 NO: 596)
T46984_PEA.sub.--1_T14 (SEQ ID 2195 2198 NO: 597)
T46984_PEA.sub.--1_T15 (SEQ ID 2112 2115 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4057 4060 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3333 3336 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2013 2016 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2019 2022 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1765 1768 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1923 1926 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1201 1204 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1515 1518 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 954 957 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 551 554 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1054 1057 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1054 1057 NO: 613)
[2658] Segment cluster T46984_PEA.sub.--1_node.sub.--83 (SEQ ID
NO:660) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T15 (SEQ ID NO:598), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T43 (SEQ
ID NO:607), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 94 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01117 TABLE 94 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 3433 3437 593)
T46984_PEA.sub.--1_T3 (SEQ ID NO: 2827 2831 594)
T46984_PEA.sub.--1_T12 (SEQ ID 2227 2231 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2258 2262 NO: 596)
T46984_PEA.sub.--1_T15 (SEQ ID 2116 2120 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4061 4065 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3337 3341 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2017 2021 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2023 2027 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1769 1773 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1927 1931 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1205 1209 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1519 1523 NO: 608).
T46984_PEA.sub.--1_T47 (SEQ ID 958 962 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 555 559 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1058 1062 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1058 1062 NO: 613)
[2659] Segment cluster T46984_PEA.sub.--1_node.sub.--84 (SEQ ID
NO:661) according to the present invention can be found in the
following transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T15 (SEQ ID NO:598), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T43 (SEQ
ID NO:607), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 95 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01118 TABLE 95 Segment location on transcripts
Segment Segment Transcript name starting position ending position
T46984_PEA.sub.--1_T2 (SEQ ID NO: 3438 3451 593)
T46984_PEA.sub.--1_T3 (SEQ ID NO: 2832 2845 594)
T46984_PEA.sub.--1_T12 (SEQ ID 2232 2245 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2263 2276 NO: 596)
T46984_PEA.sub.--1_T15 (SEQ ID 2121 2134 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4066 4079 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3342 3355 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2022 2035 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2028 2041 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1774 1787 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1932 1945 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1210 1223 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1524 1537 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 963 976 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 560 573 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1063 1076 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1063 1076 NO: 613)
[2660] Segment cluster T46984_PEA.sub.--1_node.sub.--85 (SEQ ID
NO:662) according to the present invention is supported by 295
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T46984_PEA.sub.--1_T2 (SEQ ID NO:593),
T46984_PEA.sub.--1_T3 (SEQ ID NO:594), T46984_PEA.sub.--1_T12 (SEQ
ID NO:595), T46984_PEA.sub.--1_T13 (SEQ ID NO:596),
T46984_PEA.sub.--1_T15 (SEQ ID NO:598), T46984_PEA.sub.--1_T19 (SEQ
ID NO:599), T46984_PEA.sub.--1_T23 (SEQ ID NO:600),
T46984_PEA.sub.--1_T27 (SEQ ID NO:601), T46984_PEA.sub.--1_T32 (SEQ
ID NO:602), T46984_PEA.sub.--1_T34 (SEQ ID NO:603),
T46984_PEA.sub.--1_T35 (SEQ ID NO:604), T46984_PEA.sub.--1_T43 (SEQ
ID NO:607), T46984_PEA.sub.--1_T46 (SEQ ID NO:608),
T46984_PEA.sub.--1_T47 (SEQ ID NO:609), T46984_PEA.sub.--1_T51 (SEQ
ID NO:611), T46984_PEA.sub.--1_T52 (SEQ ID NO:612) and
T46984_PEA.sub.--1_T54 (SEQ ID NO:613). Table 96 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-011 19 TABLE 96 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T46984_PEA.sub.--1_T2 (SEQ ID NO: 3452 3491 593)
T46984_PEA.sub.--1_T3 (SEQ ID NO: 2846 2885 594)
T46984_PEA.sub.--1_T12 (SEQ ID 2246 2285 NO: 595)
T46984_PEA.sub.--1_T13 (SEQ ID 2277 2316 NO: 596)
T46984_PEA.sub.--1_T15 (SEQ ID 2135 2174 NO: 598)
T46984_PEA.sub.--1_T19 (SEQ ID 4080 4119 NO: 599)
T46984_PEA.sub.--1_T23 (SEQ ID 3356 3395 NO: 600)
T46984_PEA.sub.--1_T27 (SEQ ID 2036 2075 NO: 601)
T46984_PEA.sub.--1_T32 (SEQ ID 2042 2081 NO: 602)
T46984_PEA.sub.--1_T34 (SEQ ID 1788 1827 NO: 603)
T46984_PEA.sub.--1_T35 (SEQ ID 1946 1985 NO: 604)
T46984_PEA.sub.--1_T43 (SEQ ID 1224 1263 NO: 607)
T46984_PEA.sub.--1_T46 (SEQ ID 1538 1577 NO: 608)
T46984_PEA.sub.--1_T47 (SEQ ID 977 1016 NO: 609)
T46984_PEA.sub.--1_T51 (SEQ ID 574 613 NO: 611)
T46984_PEA.sub.--1_T52 (SEQ ID 1077 1116 NO: 612)
T46984_PEA.sub.--1_T54 (SEQ ID 1077 1116 NO: 613)
[2661] Variant protein alignment to the previously known
protein:
[2662] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2663] Sequence Documentation:
[2664] Alignment of: T46984_PEA.sub.--1_P2 (SEQ ID
NO:664).times.RIB2_HUMAN (SEQ ID NO:663)
[2665] Alignment segment 1/1: TABLE-US-011 20 Quality: 4716.00
Escore: 0 Matching length: 498 Total length: 498 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00099 Alignment: TABLE-US-01121 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400 401
KAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFV 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
KAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFV 450 451
AEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNV 498
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
AEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNV 498
[2666] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2667] Sequence Documentation:
[2668] Alignment of: T46984_PEA.sub.--1_P3 (SEQ ID
NO:665).times.RIB2_HUMAN (SEQ ID NO:663).
[2669] Alignment segment 1/1: TABLE-US-01122 Quality: 4085.00
Escore: 0 Matching length: 433 Total length: 433 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
[2670] Alignment:
[2671] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2672] Sequence Documentation:
[2673] Alignment of: T46984_PEA.sub.--1_P10 (SEQ ID
NO:666).times.RIB2_HUMAN (SEQ ID NO:663).
[2674] Alignment segment 1/1: TABLE-US-01123 Quality: 4716.00
Escore: 0 Matching length: 498 Total length: 498 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00100 Alignment: TABLE-US-01124 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400 401
KAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFV 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
KAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFV 450 451
AEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNV 498
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
AEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNV 498
[2675] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2676] Sequence Documentation:
[2677] Alignment of: T46984_PEA.sub.--1_P11 (SEQ ID
NO:667).times.RIB2_HUMAN (SEQ ID NO:663).
[2678] Alignment segment 1/1: TABLE-US-011 25 Quality: 5974.00
Escore: 0 Matching length: 628 Total length: 628 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
[2679] Alignment:
[2680] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2681] Sequence Documentation:
[2682] Alignment of: T46984_PEA.sub.--1_P12 (SEQ ID
NO:668).times.RIB2_HUMAN (SEQ ID NO:663).
[2683] Alignment segment 1/1: TABLE-US-01126 Quality: 3179.00
Escore: 0 Matching length: 338 Total length: 338 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
[2684] Alignment:
[2685] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2686] Sequence Documentation:
[2687] Alignment of: T46984_PEA.sub.--1_P21 (SEQ ID
NO:669).times.RIB2_HUMAN (SEQ ID NO:663).
[2688] Alignment segment 1/1: TABLE-US-01127 Quality: 5348.00
Escore: 0 Matching length: 562 Total length: 562 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00101 Alignment TABLE-US-01128 2
KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSED 51
|||||||||||||||||||||||||||||||||||||||||||||||||| 70
KACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSED 119 52
SSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTAS 101
|||||||||||||||||||||||||||||||||||||||||||||||||| 120
SSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTAS 169 102
HLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLM 151
|||||||||||||||||||||||||||||||||||||||||||||||||| 170
HLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLM 219 152
DHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHV 201
|||||||||||||||||||||||||||||||||||||||||||||||||| 220
DHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHV 269 202
PVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATV 251
|||||||||||||||||||||||||||||||||||||||||||||||||| 270
PVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATV 319 252
LQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKI 301
|||||||||||||||||||||||||||||||||||||||||||||||||| 320
LQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKI 369 302
STEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQ 351
|||||||||||||||||||||||||||||||||||||||||||||||||| 370
STEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQ 419 352
LVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERK 401
|||||||||||||||||||||||||||||||||||||||||||||||||| 420
LVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERK 469 402
IEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNL 451
|||||||||||||||||||||||||||||||||||||||||||||||||| 470
IEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNL 519 452
FTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALWIRIGANVSN 501
|||||||||||||||||||||||||||||||||||||||||||||||||| 520
FTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALWIRIGANVSN 569 502
FTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNR 551
|||||||||||||||||||||||||||||||||||||||||||||||||| 570
FTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNR 619 552
MLAQQAVKRTAH 563 |||||||||||| 620 MLAQQAVKRTAH 631
[2689] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2690] Sequence Documentation:
[2691] Alignment of: T46984_PEA.sub.--1_P27 (SEQ ID
NO:670).times.RIB2_HUMAN (SEQ ID NO:663).
[2692] Alignment segment 1/1: TABLE-US-01129 Quality: 3910.00
Escore: 0 Matching length: 415 Total length: 415 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00102 Alignment TABLE-US-01130 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
EVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPA 400 401
KAKGTFIADSHQNFA 415 ||||||||||||||| 401 KAKGTFIADSHQNFA 415
[2693] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2694] Sequence Documentation:
[2695] Alignment of: T46984_PEA.sub.--1_P32 (SEQ ID
NO:671).times.RIB2_HUMAN (SEQ ID NO:663).
[2696] Alignment segment 1/1: TABLE-US-01131 Quality: 3434.00
Escore: 0 Matching length: 373 Total length: 373 Matching Percent
98.93 Matching Percent Identity: 98.39 Similarity: Total Percent
Similarity: 98.93 Total Percent Identity: 98.39 Gaps: 0
TABLE-US-00103 Alignment TABLE-US-01132 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
TQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLV 350 351
EVEGDNRYIANTVEGQVRWLTPV 373 |||||||||||||| :|: | | 351
EVEGDNRYIANTVELRVKISTEV 373
[2697] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2698] Sequence Documentation:
[2699] Alignment of: T46984_PEA.sub.-1_P34 (SEQ ID
NO:672).times.RIB2_HUMAN (SEQ ID NO:663).
[2700] Alignment segment 1/1: TABLE-US-01133 Quality: 3087.00
Escore: 0 Matching length: 329 Total length: 329 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00104 Alignment: TABLE-US-01134 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQTYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQTYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPL 300 301
TQATVKLEHAKSVASRATVLQKTSFTPVG 329 ||||||||||||||||||||||||||||| 301
TQATVKLEHAKSVASRATVLQKTSFTPVG 329
[2701] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2702] Sequence Documentation:
[2703] Alignment of: T46984_PEA.sub.--1_P35 (SEQ ID
NO:673).times.RIB2_HUMAN (SEQ ID NO:663).
[2704] Alignment segment 1/1: TABLE-US-011 35 Quality: 2697.00
Escore: 0 Matching length: 287 Total length: 287 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00105 Alignment TABLE-US-01136 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
RLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQ 200 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
FEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLS 250 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI 287
||||||||||||||||||||||||||||||||||||| 251
EAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAI 287
[2705] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2706] Sequence Documentation:
[2707] Alignment of: T46984_PEA.sub.--1_P38 (SEQ ID
NO:674).times.RIB2_HUMAN (SEQ ID NO:663).
[2708] Alignment segment 1/1: TABLE-US-01137 Quality: 1368.00
Escore: 0 Matching length: 145 Total length: 145 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00106 Alignment: TABLE-US-01138 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEAL 145
||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEAL 145
[2709] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2710] Sequence Documentation:
[2711] Alignment of: T46984_PEA.sub.--1_P39 (SEQ ID
NO:675).times.RIB2_HUMAN (SEQ ID NO:663).
[2712] Alignment segment 1/1: TABLE-US-011 39 Quality: 1500.00
Escore: 0 Matching length: 160 Total length: 160 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00107 Alignment TABLE-US-01140 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
EISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTA 150 151
RLSKEETVLA 160 |||||||||| 151 RLSKEETVLA 160
[2713] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2714] Sequence Documentation:
[2715] Alignment of: T46984_PEA.sub.--1_P45 (SEQ ID
NO:676).times.RIB2_HUMAN (SEQ ID NO:663)
[2716] Alignment segment 1/1: TABLE-US-01141 Quality: 970.00
Escore: 0 Matching length: 103 Total length: 103 Matching Percent
99.03 Matching Percent Identity: 99.03 Similarity: Total Percent
Similarity: 99.03 Total Percent Identity: 99.03 Gaps: 0
TABLE-US-00108 Alignment TABLE-US-01142 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
AFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGC 100 101 ENS 103
| | 101 EIS 103
[2717] Sequence name: RIB2_HUMAN (SEQ ID NO:663)
[2718] Sequence Documentation:
[2719] Alignment of: T46984_PEA.sub.--1_P46 (SEQ ID
NO:677).times.RIB2_HUMAN (SEQ ID NO:663).
[2720] Alignment segment 1/1: TABLE-US-01143 Quality: 656.00
Escore: 0 Matching length: 69 Total length: 69 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00109 Alignment TABLE-US-01144 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRPFTNLES 50 51
AFYSIVGLSSLGAQVPDAK 69 ||||||||||||||||||| 51 AFYSIVGLSSLGAQVPDAK
69
Description for Cluster T11628
[2721] Cluster T11628 features 6 transcript(s) and 25 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-01145 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. T11628_PEA.sub.--1_T3 678 T11628_PEA.sub.--1_T4 679
T11628_PEA.sub.--1_T5 680 T11628_PEA.sub.--1_T7 681
T11628_PEA.sub.--1_T9 682 T11628_PEA.sub.--1_T11 683
[2722] TABLE-US-01146 TABLE 2 Segments of interest Segment Name
Sequence ID No. T11628_PEA.sub.--1_node.sub.--7 684
T11628_PEA.sub.--1_node.sub.--11 685
T11628_PEA.sub.--1_node.sub.--16 686
T11628_PEA.sub.--1_node.sub.--22 687
T11628_PEA.sub.--1_node.sub.--25 688
T11628_PEA.sub.--1_node.sub.--31 689
T11628_PEA.sub.--1_node.sub.--37 690
T11628_PEA.sub.--1_node.sub.--0 691 T11628_PEA.sub.--1_node.sub.--4
692 T11628_PEA.sub.--1_node.sub.--9 693
T11628_PEA.sub.--1_node.sub.--13 694
T11628_PEA.sub.--1_node.sub.--14 695
T11628_PEA.sub.--1_node.sub.--17 696
T11628_PEA.sub.--1_node.sub.--18 697
T11628_PEA.sub.--1_node.sub.--19 698
T11628_PEA.sub.--1_node.sub.--24 699
T11628_PEA.sub.--1_node.sub.--27 700
T11628_PEA.sub.--1_node.sub.--28 701
T11628_PEA.sub.--1_node.sub.--29 702
T11628_PEA.sub.--1_node.sub.--30 703
T11628_PEA.sub.--1_node.sub.--32 704
T11628_PEA.sub.--1_node.sub.--33 705
T11628_PEA.sub.--1_node.sub.--34 706
T11628_PEA.sub.--1_node.sub.--35 707
T11628_PEA.sub.--1_node.sub.--36 708
[2723] TABLE-US-01147 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) T11628_PEA.sub.--1_P2 712
T11628_PEA.sub.--1_T3 (SEQ ID NO: 678); T11628_PEA.sub.--1_T5 (SEQ
ID NO: 680); T11628_PEA.sub.--1_T7 (SEQ ID NO: 681)
T11628_PEA.sub.--1_P5 713 T11628_PEA.sub.--1_T9 (SEQ ID NO: 682)
T11628_PEA.sub.--1_P7 714 T11628_PEA.sub.--1_T11 (SEQ ID NO: 683)
T11628_PEA.sub.--1_P10 715 T11628_PEA.sub.--1_T4 (SEQ ID NO:
679)
[2724] These sequences are variants of the known protein Myoglobin
(SwissProt accession identifier MYG_HUMAN), SEQ ID NO: 709,
referred to herein as the previously known protein.
[2725] Protein Myoglobin (SEQ ID NO:709) is known or believed to
have the following function(s): Serves as a reserve supply of
oxygen and facilitates the movement of oxygen within muscles. The
sequence for protein Myoglobin (SEQ ID NO:709) is given at the end
of the application, as "Myoglobin (SEQ ID NO:709) amino acid
sequence". Known polymorphisms for this sequence are as shown in
Table 4. TABLE-US-01148 TABLE 4 Amino acid mutations for Known
Protein SNP position(s) on amino acid sequence Comment 54 E->K.
/FTId=VAR.sub.--003180.133 K->N. /FTId=VAR.sub.--003181.139
R->Q. /FTId=VAR.sub.--003182.139 R->W.
/FTId=VAR.sub.--003183.128 Q->E
[2726] As noted above, cluster T11628 features 6 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Myoglobin (SEQ ID
NO:709). A description of each variant protein according to the
present invention is now provided.
[2727] Variant protein T11628_PEA.sub.--1_P2 (SEQ ID NO:712)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T11628_PEA.sub.--1_T3 (SEQ ID NO:678). An alignment is given to the
known protein (Myoglobin (SEQ ID NO:709)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2728] Comparison report between T11628_PEA.sub.--1_P2 (SEQ ID
NO:712) and Q8WVH6 (SEQ ID NO:711) (SEQ ID NO:711):
[2729] 1.An isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1.sub.--P2 (SEQ ID NO:712), comprising a first
amino acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE
(SEQ ID NO:956) corresponding to amino acids 1-55 of
T11628_PEA.sub.--1_P2 (SEQ ID NO:712), and a second amino acid
sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 1-99 of Q8WVH6 (SEQ ID NO:711), which also corresponds to
amino acids 56-154 of T11628_PEA.sub.--1_P2 (SEQ ID NO:712),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[2730] 2.An isolated polypeptide encoding for a head of
T11628_PEA.sub.--1_P2 (SEQ ID NO:712), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01149
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE (SEQ ID
NO:956) of T11628_PEA.sub.--1_P2. (SEQ ID NO:712)
[2731] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: intracellularly. The protein localization
is believed to be intracellularly because neither of the
trans-membrane region prediction programs predicted a
trans-membrane region for this protein. In addition both
signal-peptide prediction programs predict that this protein is a
non-secreted protein.
[2732] Variant protein T11628_PEA.sub.--1_P2 (SEQ ID NO:712) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 5, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T11628_PEA.sub.--1_P2 (SEQ ID
NO:712) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01150
TABLE 5 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 26
G->No 44 F->No 92 Q->R No 135 A->No 141 K->No 153
Q->No
[2733] Variant protein T11628_PEA.sub.--1_P2 (SEQ ID NO:712) is
encoded by the following transcript(s): T11628_PEA.sub.--1_T3 (SEQ
ID NO:678), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T11628_PEA.sub.--1_T3 (SEQ ID NO:678) is shown in bold; this coding
portion starts at position 220 and ends at position 681. The
transcript also has the following SNPs as listed in Table 6 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T11628_PEA.sub.--1_P2 (SEQ ID NO:712) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01151 TABLE 6 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 83 G->A Yes 93 G->A Yes 95 G->A Yes
146 G->A Yes 295 G->No 349 T->No 393 G->A Yes 423
C->T Yes 494 A->G No 498 G->A No 623 C->No 642 G->No
678 G->No 686 C->No 686 C->A No 717 C->No 787 T->G
No 820 G->T No 826 G->T No 850 C->No 934 T->G No 975
A->G Yes 1117 G->No 1218 A->G No
[2734] Variant protein T11628_PEA.sub.--1_P5 (SEQ ID NO:713)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T11628_PEA.sub.--1_T9 (SEQ ID NO:682). An alignment is given to the
known protein (Myoglobin (SEQ ID NO:709)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2735] Comparison report between T11628_PEA.sub.--1_P5 (SEQ ID
NO:713) and MYG_HUMAN.sub.--V1 (SEQ ID NO:710):
[2736] 1.An isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P5 (SEQ ID NO:713), comprising a first amino
acid sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 56-154 of MYG_HUMAN_V1 (SEQ ID NO:710), which also
corresponds to amino acids 1-99 of T11628_PEA.sub.--1_P5 (SEQ ID
NO:713).
[2737] It should be noted that the known protein sequence
(MYG_HUMAN (SEQ ID NO:709)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for MYG_HUMAN_V1 (SEQ ID NO: 710). These
changes were previously known to occur and are listed in the table
below. TABLE-US-01152 TABLE 7 Changes to MYG_HUMAN_V1 (SEQ ID NO:
710) SNP position(s) on amino acid sequence Type of change 1
init_met
[2738] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: intracellularly. The protein localization
is believed to be intracellularly because neither of the
trans-membrane region prediction programs predicted a
trans-membrane region for this protein. In addition both
signal-peptide prediction programs predict that this protein is a
non-secreted protein.
[2739] Variant protein T11628_PEA.sub.--1_P5 (SEQ ID NO:713) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T11628_PEA.sub.--1_P5 (SEQ ID
NO:713) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01153
TABLE 8 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 37 Q->R
No 80 A->No 86 K->No 98 Q->No
[2740] Variant protein T11628_PEA.sub.--1_P5 (SEQ ID NO:713) is
encoded by the following Transcript(s): T11628_PEA.sub.--1_T9 (SEQ
ID NO:682), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) is shown in bold; this coding
portion starts at position 211 and ends at position 507. The
transcript also has the following SNPs as listed in Table 9 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T11628_PEA.sub.--1_P5 (SEQ ID NO:713) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01154 TABLE 9 Nucleic acid SNPs
SNP position on nucleotide sequence Alternative nucleic acid
Previously known SNP? 2 C->T Yes 175 T->No 219 G->A Yes
249 C->T Yes 320 A->G No 324 G->A No 449 C->No 468
G->No 504 G->No 512 C->No 512 C->A No 543 C->No 613
T->G No 646 G->T No 652 G->T No 676 C->No 760 T->G
No 801 A->G Yes 943 G->No 1044 A->G No
[2741] Variant protein T11628_PEA.sub.--1_P7 (SEQ ID NO:714)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T11628_PEA.sub.--1_T11 (SEQ ID NO:683). An alignment is given to
the known protein (Myoglobin (SEQ ID NO:709)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2742] Comparison report between T11628_PEA.sub.--1_P7 (SEQ ID
NO:714) and MYG_HUMAN_V1 (SEQ ID NO:710):
[2743] 1. An isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P7 (SEQ ID NO:714), comprising a first amino
acid sequence being at least 90% homologous to
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMK
ASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQ
SKHPGDFGADAQGAMNK corresponding to amino acids 1-134 of
MYG_HUMAN_V1, which also corresponds to amino acids 1-134 of
T11628_PEA.sub.--1_P7 (SEQ ID NO:714), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence G
corresponding to amino acids 135-135 of T11628_PEA.sub.--1_P7 (SEQ
ID NO:714), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[2744] It should be noted that the known protein sequence
(MYG_HUMAN (SEQ ID NO:709)) has one or more changes than the
sequence given at the end of the application and named as being the
amino acid sequence for MYG_HUMAN_V1 (SEQ ID NO:710). These changes
were previously known to occur and are listed in the table below.
TABLE-US-01155 TABLE 10 Changes to MYG_HUMAN_V1 (SEQ ID NO: 710)
SNP position(s) on amino acid sequence Type of change 1
init_met
[2745] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: intracellularly. The protein localization
is believed to be intracellularly because neither of the
trans-membrane region prediction programs predicted a
trans-membrane region for this protein. In addition both
signal-peptide prediction programs predict that this protein is a
non-secreted protein.
[2746] Variant protein T11628_PEA.sub.--1_P7 (SEQ ID NO:714) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T11628_PEA.sub.--1_P7 (SEQ ID
NO:714) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-011
56 TABLE 11 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 26
G->No 44 F->No 92 Q->R No
[2747] Variant protein T11628_PEA.sub.--1_P7 (SEQ ID NO:714) is
encoded by the following transcript(s): T11628_PEA.sub.--1_T11 (SEQ
ID NO:683), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T11628_PEA.sub.--1_T11 (SEQ ID NO:683) is shown in bold; this
coding portion starts at position 319 and ends at position 723. The
transcript also has the following SNPs as listed in Table 12 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T11628_PEA.sub.--1_P7 (SEQ ID NO:714) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01157 TABLE 12 Nucleic acid
SNPs SNP position on nucleotide sequence Alternative nucleic acid
Previously known SNP? 394 G->No 448 T->No 492 G->A Yes 522
C->T Yes 593 A->G No 597 G->A No 728 C->No 728 C->A
No 759 C->No 829 T->G No 862 G->T No 868 G->T No 892
C->No 976 T->G No 1017A->G Yes 1159 G->No 1260A->G
No
[2748] Variant protein T11628_PEA.sub.--1_P10 (SEQ ID NO:715)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
T11628_PEA.sub.--1_T4 (SEQ ID NO:679). An alignment is given to the
known protein (Myoglobin (SEQ ID NO:709)) at the end of the
application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2749] Comparison report between T11628_PEA.sub.--1_P10 (SEQ ID
NO:715) and Q8WVH6 (SEQ ID NO:711):
[2750] 1.An isolated chimeric polypeptide encoding for
T11628_PEA.sub.--1_P10 (SEQ ID NO:715), comprising a first amino
acid sequence being at least 70%, optionally at least 80%,
preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE
(SEQ ID NO:956) corresponding to amino acids 1-55 of
T11628_PEA.sub.--1_P10 (SEQ ID NO:715), and a second amino acid
sequence being at least 90% homologous to
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQV
LQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG corresponding to amino
acids 1-99 of Q8WVH6 (SEQ ID NO:711), which also corresponds to
amino acids 56-154 of T11628_PEA.sub.--1_P10 (SEQ ID NO:715),
wherein said first amino acid sequence and second amino acid
sequence are contiguous and in a sequential order.
[2751] 2.An isolated polypeptide encoding for a head of
T11628_PEA.sub.--1_P10 (SEQ ID NO:715), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01158
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDE (SEQ ID
NO:956) of T11628_PEA.sub.--1_P10. (SEQ ID NO:715)
[2752] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: intracellularly. The protein localization
is believed to be intracellularly because neither of the
trans-membrane region prediction programs predicted a
trans-membrane region for this protein. In addition both
signal-peptide prediction programs predict that this protein is a
non-secreted protein.
[2753] Variant protein T11628_PEA.sub.--1_P10 (SEQ ID NO:715) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein T11628_PEA.sub.--1_P10 (SEQ ID
NO:715) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01159
TABLE 13 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 26
G->No 44 F->No 92 Q->R No 135 A->No 141 K->No 153
Q->No
[2754] Variant protein T11628_PEA.sub.--1_P10 (SEQ ID NO:715) is
encoded by the following transcript(s): T11628_PEA.sub.--1_T4 (SEQ
ID NO:679), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
T11628_PEA.sub.--1_T4 (SEQ ID NO:679) is shown in bold; this coding
portion starts at position 205 and ends at position 666. The
transcript also has the following SNPs as listed in Table 14 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein T11628_PEA.sub.--1_P10 (SEQ ID NO:715) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01160 TABLE 14 Nucleic acid
SNPs SNP position on nucleotide sequence Alternative nucleic acid
Previously known SNP? 280 G->No 334 T->No 378 G->A Yes 408
C->T Yes 479 A->G No 483 G->A No 608 C->No 627 G->No
663 G->No 671 C->No 671 C->A No 702 C->No 772 T->G
No 805 G->T No 811 G->T No835C->No919T->G No 960
A->G Yes 1102G->No 1203 A->G No
[2755] As noted above, cluster T11628 features 25 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[2756] Segment cluster T11628_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:684) according to the present invention is supported by 9
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678). Table 15
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01161 TABLE 15 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T11628_PEA.sub.--1_T3 (SEQ ID NO: 1211 678)
[2757] Segment cluster T11628_PEA.sub.--1_node.sub.--11 (SEQ ID
NO:685) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T5 (SEQ ID NO:680). Table 16
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01162 TABLE 16 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T11628_PEA.sub.--1_T5 (SEQ ID NO: 48 178 680)
[2758] Segment cluster T11628_PEA.sub.--1_node.sub.--16 (SEQ ID
NO:686) according to the present invention is supported by 38
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T11 (SEQ ID NO:683). Table 17
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01163 TABLE 17 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T11628_PEA.sub.--1_T11 (SEQ ID 1 214 NO: 683)
[2759] Segment cluster T11628_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:687) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T9 (SEQ ID NO:682). Table 18
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01164 TABLE 18 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T11628_PEA.sub.--1_T9 (SEQ ID NO: 1140 682)
[2760] Segment cluster T11628_PEA.sub.--1_node.sub.--25 (SEQ ID
NO:688) according to the present invention is supported by 129
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 19 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01165 TABLE
19 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 395 537 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 380
522 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 362 504
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 347 489
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 221 363
T11628_PEA.sub.--1_T11 (SEQ ID 494 636 NO: 683)
[2761] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 20. TABLE-US-01166 TABLE
20 Oligonucleotides related to this segment Oligonucleotide name
Overexpressed in cancers Chip reference
T11628.sub.--0.sub.--9.sub.--0 (SEQ ID breast malignant tumors BRS
NO: 911)
[2762] Segment cluster T11628_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:689) according to the present invention is supported by 137
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 21 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01167 TABLE
21 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 702 831 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 687
816 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 669 798
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 654 783
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 528 657
T11628_PEA.sub.--1_T11 (SEQ ID 744 873 NO: 683)
[2763] Segment cluster T11628_PEA.sub.--1_node.sub.--37 (SEQ ID
NO:690) according to the present invention is supported by 99
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 22 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01168 TABLE
22 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 1086 1225 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679)
1071 1210 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 1053 1192
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681)1038 1177
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 912 1051
T11628_PEA.sub.--1_T11 (SEQ ID 1128 1267 NO: 683)
[2764] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2765] Segment cluster T11628_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:691) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T4 (SEQ ID NO:679). Table 23
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01169 TABLE 23 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 1 93
[2766] Segment cluster T11628_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:692) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T4 (SEQ ID NO:679). Table 24
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01170 TABLE 24 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 94 196
[2767] Segment cluster T11628_PEA.sub.--1_node.sub.--9 (SEQ ID
NO:693) according to the present invention is supported by 16
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T5 (SEQ ID NO:680) and
T11628_PEA.sub.--1_T7 (SEQ ID NO:681). Table 25 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-01171 TABLE 25 Segment location on transcripts Segment
Segment ending Transcript name starting position position
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 1 47 T11628_PEA.sub.--1_T7
(SEQ ID NO: 681) 1 47
[2768] Segment cluster T11628_PEA.sub.--1_node.sub.--13 (SEQ ID
NO:694) according to the present invention can be found in the
following transcript(s): T11628_PEA.sub.--1_T7 (SEQ ID NO:681).
Table 26 below describes the starting and ending position of this
segment on each transcript. TABLE-US-01172 TABLE 26 Segment
location on transcripts Segment Segment ending Transcript name
starting position position T11628_PEA.sub.--1_T7 (SEQ ID NO: 681)
48 65
[2769] Segment cluster T11628_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:695) according to the present invention is supported by 1
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T7 (SEQ ID NO:681). Table 27
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01173 TABLE 27 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) .delta.
163
[2770] Segment cluster T11628_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:696) according to the present invention is supported by 55
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T11 (SEQ ID NO:683). Table 28
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01174 TABLE 28 Segment location on
transcripts Segment Segment Transcript name starting position
ending position T11628_PEA.sub.--1_T11 (SEQ ID 215 310 NO: 683)
[2771] Segment cluster T11628_PEA.sub.--1_node.sub.--18 (SEQ ID
NO:697) according to the present invention is supported by 98
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681) and
T11628_PEA.sub.--1_T11 (SEQ ID NO:683). Table 29 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01175 TABLE 29 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) 212 289
T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 197 274
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 179 256
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 164 241
T11628_PEA.sub.--1_T11 (SEQ ID NO: 683) 311 388
[2772] Segment cluster T11628_PEA.sub.--1_node.sub.--19 (SEQ ID
NO:698) according to the present invention can be found in the
following transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681) and
T11628_PEA.sub.--1_T11 (SEQ ID NO:683). Table 30 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01176 TABLE 30 Segment location on transcripts
Segment Segment ending Transcript name starting position position
T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) 290 314
T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 275 299
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 257 281
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 242 266
T11628_PEA.sub.--1_T11 (SEQ ID NO: 683) 389 413
[2773] Segment cluster T11628_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:699) according to the present invention is supported by 112
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01177 TABLE
31 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 315 394 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 300
379 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 282 361
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 267 346
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 141 220
T11628_PEA.sub.--1_T11 (SEQ ID 414 493 NO: 683)
[2774] Segment cluster T11628_PEA.sub.--1_node.sub.--27 (SEQ ID
NO:700) according to the present invention is supported by 119
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 32 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01178 TABLE
32 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 538 621 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 523
606 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 505 588
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 490 573
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 364 447
T11628_PEA.sub.--1_T11 (SEQ ID 637 720 NO: 683)
[2775] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 33. TABLE-US-01179 TABLE
33 Oligonucleotides related to this segment Oligonucleotide name
Overexpressed in cancers Chip reference
T11628.sub.--0.sub.--9.sub.--0 (SEQ ID breast malignant tumors BRS
NO: 911)
[2776] Segment cluster T11628_PEA.sub.--1_node.sub.--28 (SEQ ID
NO:701) according to the present invention is supported by 115
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681) and
T11628_PEA.sub.--1_T9 (SEQ ID NO:682). Table 34 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-01180 TABLE 34 Segment location on transcripts Segment
Segment ending Transcript name starting position position
T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) 622 650
T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 607 635
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 589 617
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 574 602
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 448 476
[2777] Segment cluster T11628_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:702) according to the present invention is supported by 113
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681) and
T11628_PEA.sub.--1_T9 (SEQ ID NO:682). Table 35 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-011 81 TABLE 35 Segment location on transcripts Segment
Segment ending Transcript name starting position position
T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) 651 678
T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 636 663
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 618 645
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 603 630
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 477 504
[2778] Segment cluster T11628_PEA.sub.--1_node.sub.--30 (SEQ ID
NO:703) according to the present invention can be found in the
following transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 36 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01182 TABLE
36 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 679 701 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 664
686 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 646 668
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 631 653
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 505 527
T11628_PEA.sub.--1_T11 (SEQ ID 721 743 NO: 683)
[2779] Segment cluster T11628_PEA.sub.--1_node.sub.--32 (SEQ ID
NO:704) according to the present invention can be found in the
following transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 37 below describes the starting and ending
position of this segment on each transcript. TABLE-US-011 83 TABLE
37 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 832 844 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 817
829 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 799 811
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 784 796
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 658 670
T11628_PEA.sub.--1_T11 (SEQ ID 874 886 NO: 683)
[2780] Segment cluster T11628_PEA.sub.--1_node.sub.--33 (SEQ ID
NO:705) according to the present invention can be found in the
following transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 38 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01184 TABLE
38 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 845 866 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 830
851 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 812 833
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 797 818
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 671 692
T11628_PEA.sub.--1_T11 (SEQ ID 887 908 NO: 683)
[2781] Segment cluster T11628_PEA.sub.--1_node.sub.--34 (SEQ ID
NO:706) according to the present invention is supported by 122
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681), T11628_PEA1_T9
(SEQ ID NO:682) and T11628_PEA.sub.--1_T11 (SEQ ID NO:683). Table
39 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01185 TABLE 39 Segment location on
transcripts Segment Segment ending Transcript name starting
position position T11628_PEA.sub.--1_T3 (SEQ ID NO: 678) 867 911
T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 852 896
T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 834 878
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 819 863
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 693 737
T11628_PEA.sub.--1_T11 (SEQ ID 909 953 NO: 683)
[2782] Segment cluster T11628_PEA.sub.--1_node.sub.--35 (SEQ ID
NO:707) according to the present invention is supported by 126
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682 and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 40 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01186 TABLE
40 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 912 967 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679) 897
952 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 879 934
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 864 919
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 738 793
T11628_PEA.sub.--1_T11 (SEQ ID 954 1009 NO: 683)
[2783] Segment cluster T11628_PEA.sub.--1_node.sub.--36 (SEQ ID
NO:708) according to the present invention is supported by 122
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): T11628_PEA.sub.--1_T3 (SEQ ID NO:678),
T11628_PEA.sub.--1_T4 (SEQ ID NO:679), T11628_PEA.sub.--1_T5 (SEQ
ID NO:680), T11628_PEA.sub.--1_T7 (SEQ ID NO:681),
T11628_PEA.sub.--1_T9 (SEQ ID NO:682) and T11628_PEA.sub.--1_T11
(SEQ ID NO:683). Table 41 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01187 TABLE
41 Segment location on transcripts Segment Segment ending
Transcript name starting position position T11628_PEA.sub.--1_T3
(SEQ ID NO: 678) 968 1085 T11628_PEA.sub.--1_T4 (SEQ ID NO: 679)
953 1070 T11628_PEA.sub.--1_T5 (SEQ ID NO: 680) 935 1052
T11628_PEA.sub.--1_T7 (SEQ ID NO: 681) 920 1037
T11628_PEA.sub.--1_T9 (SEQ ID NO: 682) 794 911
T11628_PEA.sub.--1_T11 (SEQ ID 1010 1127 NO: 683)
[2784] Variant protein alignment to the previously known
protein:
[2785] Sequence name: Q8WVH6 (SEQ ID NO:711)
[2786] Sequence Documentation:
[2787] Alignment of: T11628_PEA.sub.--1_P2 (SEQ ID
NO:712).times.Q8WVH6 (SEQ ID NO:711).
[2788] Alignment segment 1/1: TABLE-US-01188 Quality: 962.00
Escore: 0 Matching length: 99 Total length: 99 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00110 Alignment: TABLE-US-01189 56
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 105
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 50 106
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 99
[2789] Sequence name: MYG_HUMAN_V1 (SEQ ID NO:710)
[2790] Sequence Documentation:
[2791] Alignment of: T11628_PEA.sub.--1_P5 (SEQ ID
NO:713).times.MYG_HUMAN_V1 (SEQ ID NO:710).
[2792] Alignment segment 1/1: TABLE-US-01190 Quality: 962.00
Escore: 0 Matching length: 99 Total length: 99 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00111 Alignment: TABLE-US-01191 1
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 56
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 105 51
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 99
|||||||||||||||||||||||||||||||||||||||||||||||||| 106
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
[2793] Sequence name: MYG_HUMAN_V1 (SEQ ID NO:710)
[2794] Sequence Documentation:
[2795] Sequence of: T11628_PEA.sub.--1_P7 (SEQ ID
NO:714).times.MYG_HUMAN_V1 (SEQ ID NO:710).
[2796] Alignment segment 1/1: TABLE-US-01192 Quality: 1315.00
Escore: 0 Matching length: 134 Total length: 134 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00112 Alignment: TABLE-US-01193 1
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHL 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHL 50 51
KSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKI 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
KSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKI 100 101
PVKYLEFISECIIQVLQSKHPGDFGADAQGAMNK 134
|||||||||||||||||||||||||||||||||| 101
PVKYLEFISECIIQVLQSKHPGDFGADAQGAMNK 134
[2797] Sequence name: Q8WVH6 (SEQ ID NO:711)
[2798] Sequence Documentation:
[2799] Alignment of: T11628_PEA.sub.--1_P10 (SEQ ID
NO:715).times.Q8WVH6 (SEQ ID NO:711).
[2800] Alignment segment 1/1: TABLE-US-011 94 Quality: 962.00
Escore: 0 Matching length: 99 Total length: 99 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00113 Alignment: TABLE-US-01195 56
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 105
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYL 50 106
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
EFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG 99
Description for Cluster M78076
[2801] Cluster M78076 features 9 transcript(s) and 35 segment(s) of
interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-01196 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. M78076_PEA.sub.--1_T2 716 M78076_PEA.sub.--1_T3 717
M78076_PEA.sub.--1_T5 718 M78076_PEA.sub.--1_T13 719 M78076_PEA 1
T15 720 M78076 PEA 1 T23 721 M78076 PEA 1 T26 722
M78076_PEA.sub.--1_T27 723 M78076_PEA.sub.--1_T28 724
[2802] TABLE-US-01197 TABLE 2 Segments of interest Segment Name
Sequence ID No. M78076_PEA.sub.--1_node.sub.--0 725
M78076_PEA.sub.--1_node.sub.--10 726
M78076_PEA.sub.--1_node.sub.--15 727
M78076_PEA.sub.--1_node.sub.--18 728
M78076_PEA.sub.--1_node.sub.--20 729
M78076_PEA.sub.--1_node.sub.--24 730
M78076_PEA.sub.--1_node.sub.--26 731
M78076_PEA.sub.--1_node.sub.--29 732
M78076_PEA.sub.--1_node.sub.--32 733 M78076_PEA.sub.--1_node
.about.35 734 M78076_PEA.sub.--1_node.sub.--37 735
M78076_PEA.sub.--1_node .about.46 736 M78076_PEA.sub.--1_node
.about.47 737 M78076_PEA.sub.--1_node.sub.--54 738
M78076_PEA.sub.--1_node .about.1 739
M78076_PEA.sub.--1_node.sub.--2 740 M78076_PEA.sub.--1_node.sub.--3
741 M78076_PEA.sub.--1_node.sub.--6 742
M78076_PEA.sub.--1_node.sub.--7 743
M78076_PEA.sub.--1_node.sub.--12 744
M78076_PEA.sub.--1_node.sub.--22 745
M78076_PEA.sub.--1_node.sub.--27 746
M78076_PEA.sub.--1_node.sub.--30 747
M78076_PEA.sub.--1_node.sub.--31 748
M78076_PEA.sub.--1_node.sub.--34 749
M78076_PEA.sub.--1_node.sub.--36 750
M78076_PEA.sub.--1_node.sub.--41 751
M78076_PEA.sub.--1_node.sub.--42 752
M78076_PEA.sub.--1_node.sub.--43 753
M78076_PEA.sub.--1_node.sub.--45 754
M78076_PEA.sub.--1_node.sub.--49 755
M78076_PEA.sub.--1_node.sub.--50 756
M78076_PEA.sub.--1_node.sub.--51 757
M78076_PEA.sub.--1_node.sub.--52 758
M78076_PEA.sub.--1_node.sub.--53 759
[2803] TABLE-US-01198 TABLE 3 Proteins of interest Sequence Protein
Name ID No. Corresponding Transcript(s) M78076_PEA.sub.--1_P3 761
M78076_PEA.sub.--1_T2 (SEQ ID NO: 716); M78076_PEA.sub.--1_T5 (SEQ
ID NO: 718) M78076_PEA.sub.--1_P4 762 M78076_PEA.sub.--1_T3 (SEQ ID
NO: 717) M78076_PEA.sub.--1_P12 763 M78076_PEA.sub.--1_T13 (SEQ ID
NO: 719) M78076_PEA.sub.--1_P14 764 M78076_PEA.sub.--1_T15 (SEQ ID
NO: 720) M78076_PEA.sub.--1_P21 765 M78076_PEA.sub.--1_T23 (SEQ ID
NO: 721) M78076_PEA.sub.--1_P24 766 M78076_PEA.sub.--1_T26 (SEQ ID
NO: 722) M78076_PEA.sub.--1_P2 767 M78076_PEA.sub.--1_T27 (SEQ ID
NO: 723) M78076_PEA.sub.--1_P25 768 M78076_PEA.sub.--1_T28 (SEQ ID
NO: 724)
[2804] These sequences are variants of the known protein
Amyloid-like protein 1 precursor (SwissProt accession identifier
APP1_HUMAN; known also according to the synonyms APLP; APLP-1), SEQ
ID NO: 760, referred to herein as the previously known protein.
[2805] Protein Amyloid-like protein 1 precursor (SEQ ID NO:760) is
known or believed to have the following function(s): May play a
role in postsynaptic function. The C-terminal gamma-secretase
processed fragment, ALID1, activates transcription activation
through APBB1 (Fe65) binding (By similarity). Couples to JIP signal
transduction through C-terminal binding. May interact with cellular
G-protein signaling pathways. Can regulate neurite outgrowth
through binding to components of the extracellular matrix such as
heparin and collagen 1. The gamma-CTF peptide, C30, is a potent
enhancer of neuronal apoptosis (By similarity). The sequence for
protein Amyloid-like protein 1 precursor (SEQ ID NO:760) is given
at the end of the application, as "Amyloid-like protein 1 precursor
(SEQ ID NO:760) amino acid sequence". Known polymorphisms for this
sequence are as shown in Table 4. TABLE-US-01199 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 48 A->P
[2806] Protein Amyloid-like protein 1 precursor (SEQ ID NO:760)
localization is believed to be Type I membrane protein.
C-terminally processed in the Golgi complex.
[2807] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: endocytosis;
apoptosis; cell adhesion; neurogenesis; cell death, which are
annotation(s) related to Biological Process; protein binding;
heparin binding, which are annotation(s) related to Molecular
Function; and basement membrane; coated pit; integral membrane
protein, which are annotation(s) related to Cellular Component.
[2808] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[2809] As noted above, cluster M78076 features 9 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Amyloid-like protein 1
precursor (SEQ ID NO:760). A description of each variant protein
according to the present invention is now provided.
[2810] Variant protein M78076_PEA.sub.--1_P3 (SEQ ID NO:761)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T2 (SEQ ID NO:716). An alignment is given to the
known protein (Amyloid-like protein 1 precursor (SEQ ID NO:760)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2811] Comparison report between M78076_PEA.sub.--1_P3 (SEQ ID
NO:761) and APP1_HUMAN (SEQ ID NO:760):
[2812] 1. An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P3 (SEQ ID NO:761), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKD corresponding to
amino acids 1-517 of APP1_HUMAN (SEQ ID NO:760), which also
corresponds to amino acids 1-517 of M78076_PEA.sub.--1_P3 (SEQ ID
NO:761), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence GE corresponding to amino acids
518-519 of M78076_PEA.sub.--1_P3 (SEQ ID NO:761), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2813] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2814] Variant protein M78076_PEA.sub.--1_P3 (SEQ ID NO:761) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 5, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P3 (SEQ ID
NO:761) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01200
TABLE 5 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2815] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P3 (SEQ ID NO:761), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 6 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01201 TABLE 6
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 461 551 no
[2816] Variant protein M78076_PEA.sub.--1_P3 (SEQ ID NO:761) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T2 (SEQ
ID NO:716), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T2 (SEQ ID NO:716) is shown in bold; this coding
portion starts at position 142 and ends at position 1698. The
transcript also has the following SNPs as listed in Table 7 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein M78076_PEA.sub.--1_P3 (SEQ ID NO:761) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01202 TABLE 7 Nucleic acid SNPs
SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 114 G->No 151 G->C Yes 158 C->A Yes
179 G->A Yes 219 A->G Yes 243 G->No 253 G->A Yes 315
A->G Yes 366 A->G Yes 404 C->G Yes 512 G->A Yes 522
C->No 522 C->T No 575 T->C No 781 G->No 781 G->A No
927 G->No 951 C->No 1067 G->A Yes 1077 G->A Yes 1251
G->No 1398 G->T Yes 1423 C->T Yes 2146 G->A Yes 2224
C->T No 2362 C->T Yes 2513 A->G No 2656 C->T Yes
[2817] Variant protein M78076_PEA.sub.--1_P4 (SEQ ID NO:762)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T3 (SEQ ID NO:717). An alignment is given to the
known protein (Amyloid-like protein 1 precursor (SEQ ID NO:760)) at
the end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2818] Comparison report between M78076_PEA.sub.--1_P4 (SEQ ID
NO:762) and APP1_HUMAN (SEQ ID NO:760):
[2819] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKG
corresponding to amino acids 1-526 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-526 of
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ECLTVNPSLQIPLNP (SEQ ID NO:958) corresponding to amino acids
527-541 of M78076_PEA.sub.--1_P4 (SEQ ID NO:762), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2820] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ECLTVNPSLQIPLNP (SEQ ID NO:958) in M78076_PEA.sub.--1_P4 (SEQ ID
NO:762).
[2821] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2822] Variant protein M78076_PEA.sub.--1_P4 (SEQ ID NO:762) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P4 (SEQ ID
NO:762) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01203
TABLE 8 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2823] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P4 (SEQ ID NO:762), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 9 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01204 TABLE 9
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 461 551 no
[2824] Variant protein M78076_PEA.sub.--1_P4 (SEQ ID NO:762) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T3 (SEQ
ID NO:717), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T3 (SEQ ID NO:717) is shown in bold; this coding
portion starts at position 142 and ends at position 1764. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein M78076_PEA.sub.--1_P4 (SEQ ID NO:762) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01205 TABLE 10 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 114G->No 151 G->C Yes 158 C->A Yes
179 G->A Yes 219A->G Yes 243G->No 253 G->A Yes 315
A->G Yes 366 A->G Yes 404 C->G Yes 512 G->A Yes 522
C->No 522 C->T No 575 T->C No 781 G->No 781 G->A No
927 G->No 951 C->No 1067 G->A Yes 1077 G->A Yes 1251
G->No 1398 G->T Yes 1423 C->T Yes 1817 G->A Yes 2362
G->A Yes 2440 C->T No 2578 C->T Yes 2729 A->G No 2872
C->T Yes
[2825] Variant protein M78076_PEA.sub.--1_P12 (SEQ ID NO:763)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T13 (SEQ ID NO:719). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2826] Comparison report between M78076_PEA.sub.--1_P12 (SEQ ID
NO:763) and APP1_HUMAN (SEQ ID NO:760):
[2827] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKG
corresponding to amino acids 1-526 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-526 of
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
ECVCSKGFPFPLIGDSEG (SEQ ID NO:959) corresponding to amino acids
527-544 of M78076_PEA.sub.--1_P12 (SEQ ID NO:763), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2828] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
ECVCSKGFPFPLIGDSEG (SEQ ID NO:959) in M78076_PEA.sub.--1_P12 (SEQ
ID NO:763).
[2829] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2830] Variant protein M78076_PEA.sub.--1_P12 (SEQ ID NO:763) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P12 (SEQ ID
NO:763) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01206
TABLE 11 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2831] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P12 (SEQ ID NO:763), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 12 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01207 TABLE 12
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 461 551 no
[2832] Variant protein M78076_PEA.sub.--1_P12 (SEQ ID NO:763) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T13 (SEQ
ID NO:719), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T13 (SEQ ID NO:719) is shown in bold; this
coding portion starts at position 142 and ends at position 1773.
The transcript also has the following SNPs as listed in Table 13
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P12 (SEQ ID NO:763) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01208 TABLE 13
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 114 G->No 151 G->C Yes 158
C->A Yes 179 G->A Yes 219 A->G Yes 243 G->No 253
G->A Yes 315 A->G Yes 366 A->G Yes 404 C->G Yes 512
G->A Yes 522 C->No 522 C->T No 575 T->C No 781 G->No
781 G->A No 927 G->No 951 C->No 1067 G->A Yes 1077
G->A Yes 1251 G->No 1398 G->T Yes 1423 C->T Yes 1816
G->A Yes 1894 C->T No 2032 C->T Yes 2183 A->G No 2326
C->T Yes
[2833] Variant protein M78076_PEA.sub.--1_P14 (SEQ ID NO:764)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T15 (SEQ ID NO:720). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2834] Comparison report between M78076_PEA.sub.--1_P14 (SEQ ID
NO:764) and APP1_HUMAN (SEQ ID NO:760):
[2835] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGST
EQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDEL corresponding to amino
acids 1-570 of APP1_HUMAN (SEQ ID NO:760), which also corresponds
to amino acids 1-570 of M78076_PEA.sub.--1_P14 (SEQ ID NO:764), and
a second amino acid sequence being at least 70%, optionally at
least 80%, preferably at least 85%, more preferably at least 90%
and most preferably at least 95% homologous to a polypeptide having
the sequence VRGGTAGYLGEETRGQRPGCDSQSHTGPSKKPSAPSPLPAGTSWDRGVP (SEQ
ID NO:960) corresponding to amino acids 571-619 of
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2836] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01209 VRGGTAGYLGEETRGQRPGCDSQSHTGPSKKPSAPSPLPAGTSWDRGVP
(SEQ ID NO:960) in M78076_PEA.sub.--1_P14. (SEQ ID NO:764)
[2837] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2838] Variant protein M78076_PEA.sub.--1_P14 (SEQ ID NO:764) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 14, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P14 (SEQ ID
NO:764) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01210
TABLE 14 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2839] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P14 (SEQ ID NO:764), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 15 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01211 TABLE 15
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 461 551 yes 551
[2840] Variant protein M78076_PEA.sub.--1_P14 (SEQ ID NO:764) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T1 5
(SEQ ID NO:720), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) is shown in bold; this
coding portion starts at position 142 and ends at position 1998.
The transcript also has the following SNPs as listed in Table 16
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P14 (SEQ ID NO:764) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01212 TABLE 16
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 114 G->No 151 G->C Yes 158
C->A Yes 179 G->A Yes 219 A->G Yes 243 G->No 253
G->A Yes 315 A->G Yes 366 A->G Yes 404 C->G Yes 512
G->A Yes 522 C->No 522 C->T No 575 T->C No 781 G->No
781 G->A No 927 G->No 951 C->No 1067 G->A Yes 1077
G->A Yes 1251 G->No 1398 G->T Yes 1423 C->T Yes 2008
G->A Yes 2086 C->T No 2224 C->T Yes 2375 A->G No 2518
C->T Yes
[2841] Variant protein M78076_PEA.sub.--1_P21 (SEQ ID NO:765)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T23 (SEQ ID NO:721). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2842] Comparison report between M78076_PEA.sub.--1_P21 (SEQ ID
NO:765) and APP1_HUMAN (SEQ ID NO:760):
[2843] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPMRGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN E
corresponding to amino acids 1-352 of APP1_HUMAN (SEQ ID NO:760),
which also corresponds to amino acids 1-352 of
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), and a second amino acid
sequence being at least 90% homologous to
AERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQ
SLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMT
LPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREA
VSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGWEVDPMLTLEEQQLRELQRHGYE
NPTYRFLEERP corresponding to amino acids 406-650 of APP1_HUMAN (SEQ
ID NO:760), which also corresponds to amino acids 353-597 of
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2844] 2.An isolated chimeric polypeptide encoding for an edge
portion of M78076_PEA.sub.--1_P21 (SEQ ID NO:765), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EA, having a structure as follows: a
sequence starting from any of amino acid numbers 352-x to 352; and
ending at any of amino acid numbers 353+((n-2)-x), in which x
varies from 0 to n-2.
[2845] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: membrane. The protein localization is
believed to be membrane because although both signal-peptide
prediction programs agree that this protein has a signal peptide,
both trans-membrane region prediction programs predict that this
protein has a trans-membrane region downstream of this signal
peptide.
[2846] Variant protein M78076_PEA.sub.--1_P21 (SEQ ID NO:765) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 17, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P21 (SEQ ID
NO:765) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01213
TABLE 17 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes
[2847] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P21 (SEQ ID NO:765), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 18 (given. according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01214 TABLE 18
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 408 551 yes 498
[2848] Variant protein M78076_PEA.sub.--1_P21 (SEQ ID NO:765) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T23 (SEQ ID NO:721) is shown in bold; this
coding portion starts at position 142 and ends at position 1932.
The transcript also has the following SNPs as listed in Table 19
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P21 (SEQ ID NO:765) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01215 TABLE 19
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 114 G->No 151 G->C Yes 158
C->A Yes 179 G->A Yes 219 A->G Yes 243 G->No 253
G->A Yes 315 A->G Yes 366 A->G Yes 404 C->G Yes 512
G->A Yes 522 C->No 522 C->T No 575 T->C No 781 G->No
781 G->A No 927 G->No 951 C->No 1067 G->A Yes 1077
G->A Yes 1239 G->T Yes 1264 C->T Yes 1728 G->A Yes 1806
C->T No 1944 C->T Yes 2095 A->G No 2238 C->T Yes
[2849] Variant protein M78076_PEA.sub.--1_P24 (SEQ ID NO:766)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T26 (SEQ ID NO:722). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2850] Comparison report between M78076_PEA.sub.--1_P24 (SEQ ID
NO:766) and APP1_HUMAN (SEQ ID NO:760):
[2851] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVMVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLD
QNPHLAQELRPQI corresponding to amino acids 1-481 of APP1_HUMAN (SEQ
ID NO:760), which also corresponds to amino acids 1-481 of
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), and a second amino acid
sequence being at least 70%, optionally at least 80%, preferably at
least 85%, more preferably at least 90% and most preferably at
least 95% homologous to a polypeptide having the sequence
RECLLPWLPLQISEGRS (SEQ ID NO:961) corresponding to amino acids
482-498 of M78076_PEA.sub.--1.sub.P24 (SEQ ID NO:766), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2852] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
RECLLPWLPLQISEGRS (SEQ ID NO:961) in M78076_PEA.sub.--1_P24 (SEQ ID
NO:766).
[2853] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2854] Variant protein M78076_PEA.sub.--1_P24 (SEQ ID NO:766) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 20, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P24 (SEQ ID
NO:766) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01216
TABLE 20 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2855] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P24 (SEQ ID NO:766), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 21 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01217 TABLE 21
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 yes 461 551 no
[2856] Variant protein M78076_PEA.sub.--1_P24 (SEQ ID NO:766) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T26 (SEQ
ID NO:722), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T26 (SEQ ID NO:722) is shown in bold; this
coding portion starts at position 142 and ends at position 1635.
The transcript also has the following SNPs as listed in Table 22
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P24 (SEQ ID NO:766) sequence
provides support for the deduced sequence variant protein according
to the present invention). TABLE-US-01218 TABLE 22 Nucleic acid
SNPs SNP position on nucleotide Alternative sequence nucleic acid
Previously known SNP? 114G->No 151 G->C Yes 158C->A Yes
179G->A Yes 219A->G Yes 243G->No 253 G->A Yes 315
A->G Yes 366 A->G Yes 404 C->G Yes 512 G->A Yes 522
C->No 522 C->T No 575 T->C No 781 G->No 781 G->A No
927 G->No 951 C->No 1067 G->A Yes 1077 G->A Yes 1251
G->No 1398 G->T Yes 1423 C->T Yes 2184 G->A Yes
[2857] Variant protein M78076_PEA.sub.--1_P2 (SEQ ID NO:767)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T27 (SEQ ID NO:723). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2858] Comparison report between M78076_PEA.sub.--1_P2 (SEQ ID
NO:767) and APP1_HUMAN (SEQ ID NO:760):
[2859] 1. An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P2 (SEQ ID NO:767), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQV corresponding to amino acids
1-449 of APP1_HUMAN (SEQ ID NO:760), which also corresponds to
amino acids 1-449 of M78076_PEA.sub.--1_P2 (SEQ ID NO:767), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence
LTSFQLPNAPLFLRRPRLRLFSCPLDPLSVSWTPSYPLNTASLPLPSLSAQLPDPETWTLT
CCVFDPCFLALGFLLPPPSILCSVPWIFTAFPRIVFFFFFFLRQVLALSPRQESSVRSWLIAT
STSWVQAILLPQPLE (SEQ ID NO:962) corresponding to amino acids
450-588 of M78076_PEA.sub.--1_P2 (SEQ ID NO:767), wherein said
first amino acid sequence and second amino acid sequence are
contiguous and in a sequential order.
[2860] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P2 (SEQ ID NO:767), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01219
LTSFQLPNAPLFLRRPRLRLFSCPLDPLSVSWTPSYPLNTASLPLPSLSAQLPDPETWTLT (SEQ
ID NO:962)
CCVFDPCFLALGFLLPPPSILCSVPWIFTAFPRIVFFFFFFLRQVLALSPRQESSVRSWLIAT
STSWVQAILLPQPLE in M78076_PEA.sub.--1_P2. (SEQ ID NO:767)
[2861] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: membrane. The protein localization is
believed to be membrane because although both signal-peptide
prediction programs agree that this protein has a signal peptide,
both trans-membrane region prediction programs predict that this
protein has a trans-membrane region downstream of this signal
peptide.
[2862] Variant protein M78076_PEA.sub.--1_P2 (SEQ ID NO:767) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 23, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P2 (SEQ ID
NO:767) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01220
TABLE 23 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No 520 A->S Yes 546 F->Yes 564 S->C Yes
[2863] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P2 (SEQ ID NO:767), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 24 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01221 TABLE 24
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 no 551 no
[2864] Variant protein M78076_PEA.sub.--1_P2 (SEQ ID NO:767) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T27 (SEQ
ID NO:723), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) is shown in bold; this
coding portion starts at position 142 and ends at position 1905.
The transcript also has the following SNPs as listed in Table 25
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P2 (SEQ ID NO:767) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01222 TABLE 25
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 114 G->No 151 G->C Yes 158
C->A Yes 179 G->A Yes 219A->G Yes 243 G->No 253 G->A
Yes 315 A->G Yes 366 A->G Yes 404 C->G Yes 512 G->A Yes
522 C->No 522 C->T No 575 T->C No 781 G->No 781 G->A
No 927 G->No 951 C->No 1067 G->A Yes 1077 G->A Yes 1251
G->No 1398 G->T Yes 1423 C->T Yes 1500 C->T Yes 1699
G->T Yes 1725 G->A Yes 1777 T->Yes 1831 A->T Yes 2274
A->G Yes 2525 A->G Yes 2681 G->A Yes 3831 G->A Yes
[2865] Variant protein M78076_PEA.sub.--1_P25 (SEQ ID NO:768)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
M78076_PEA.sub.--1_T28 (SEQ ID NO:724). An alignment is given to
the known protein (Amyloid-like protein 1 precursor (SEQ ID
NO:760)) at the end of the application. One or more alignments to
one or more previously published protein sequences are given at the
end of the application. A brief description of the relationship of
the variant protein according to the present invention to each such
aligned protein is as follows:
[2866] Comparison report between M78076_PEA.sub.--1_P25 (SEQ ID
NO:768) and APP1_HUMAN (SEQ ID NO:760):
[2867] 1.An isolated chimeric polypeptide encoding for
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), comprising a first amino
acid sequence being at least 90% homologous to
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGL
CGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPME
RWCGGSRSGSCAHPHHQWPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPG
SRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGV
DIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALN
EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLL
ALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQ corresponding to amino acids
1-448 of APP1_HUMAN (SEQ ID NO:760), which also corresponds to
amino acids 1-448 of M78076_PEA.sub.--1_P25 (SEQ ID NO:768), and a
second amino acid sequence being at least 70%, optionally at least
80%, preferably at least 85%, more preferably at least 90% and most
preferably at least 95% homologous to a polypeptide having the
sequence PQNPNSQPRAAGSLEVIISHPFVRRLEILISPFQFQNSIPKNSQIVPAASPRGTSSP
(SEQ ID NO:963) corresponding to amino acids 449-505 of
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[2868] 2.An isolated polypeptide encoding for a tail of
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01223
PQNPNSQPRAAGSLEVIISHPFVRRLEILISPFQFQNSIPKNSQIVPAASPRGTSSP (SEQ ID
NO:963) in M78076_PEA.sub.--1_P25. (SEQ ID NO:768)
[2869] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2870] Variant protein M78076_PEA.sub.--1_P25 (SEQ ID NO:768) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 26, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein M78076_PEA.sub.--1_P25 (SEQ ID
NO:768) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01224
TABLE 26 Amino acid mutations SNP position(s) on amino acid
Alternative sequence amino acid(s) Previously known SNP? 4 A->P
Yes 6 P->H Yes 13 R->H Yes 34 Q->No. 38 G->R Yes 88
P->R Yes 124 R->Q Yes 127 S->No 145 F->S No 214 G->R
No 214 G->No 262 Q->No 270 V->No 309 G->E Yes 370
Q->No
[2871] The glycosylation sites of variant protein
M78076_PEA.sub.--1_P25 (SEQ ID NO:768), as compared to the known
protein Amyloid-like protein 1 precursor (SEQ ID NO:760), are
described in Table 27 (given according to their position(s) on the
amino acid sequence in the first column; the second column
indicates whether the glycosylation site is present in the variant
protein; and the last column indicates whether the position is
different on the variant protein). TABLE-US-01225 TABLE 27
Glycosylation site(s) Position(s) on known amino Present in
Position acid sequence variant protein? in variant protein? 337 yes
337 461 no 551 no
[2872] Variant protein M78076_PEA.sub.--1_P25 (SEQ ID NO:768) is
encoded by the following transcript(s): M78076_PEA.sub.--1_T28 (SEQ
ID NO:724), for which the sequence(s) is/are given at the end of
the application. The coding portion of transcript
M78076_PEA.sub.--1_T28 (SEQ ID NO:724) is shown in bold; this
coding portion starts at position 142 and ends at position 1656.
The transcript also has the following SNPs as listed in Table 28
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein M78076_PEA.sub.--1_P25 (SEQ ID NO:768) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01226 TABLE 28
Nucleic acid SNPs SNP position on nucleotide Alternative sequence
nucleic acid Previously known SNP? 114 G->No 151 G->C Yes 158
C->A Yes 179 G->A Yes 219A->G Yes 243 G->No 253 G->A
Yes 315 A->G Yes 366 A->G Yes 404 C->G Yes 512 G->A Yes
522 C->No 522 C->T No 575 T->C No 781 G->No 781 G->A
No 927 G->No 951 C->No 1067 G->A Yes 1077 G->A Yes 1251
G->No 1398 G->T Yes 1423 C->T Yes 1593A->G No 1736
C->T Yes
[2873] As noted above, cluster M78076 features 35 segment(s), which
were listed in Table 2 above and for which the sequence(s) are
given at the end of the application. These segment(s) are portions
of nucleic acid sequence(s) which are described herein separately
because they are of particular interest. A description of each
segment according to the present invention is now provided.
[2874] Segment cluster M78076_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:725) according to the present invention is supported by 47
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 29 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01227 TABLE
29 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 1
160 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 1 160 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1 160 NO: 718) M78076_PEA.sub.--1_T13
(SEQ ID 1 160 NO: 719) M78076_PEA.sub.--1_T15 (SEQ ID 1 160 NO:
720) M78076_PEA.sub.--1_T23 (SEQ ID 1 160 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 1 160 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 1 160 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 1 160 NO: 724)
[2875] Segment cluster M78076_PEA.sub.--1_node.sub.--10 (SEQ ID
NO:726) according to the present invention is supported by 70
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 30 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01228 TABLE
30 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 433
565 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 433 565 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 433 565 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 433 565 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 433 565 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 433 565 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 433 565 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 433 565 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 433 565 NO: 724)
[2876] Segment cluster M78076_PEA.sub.--1_node.sub.--15 (SEQ ID
NO:727) according to the present invention is supported by 74
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 31 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01229 TABLE
31 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 679
812 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 679 812 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 679 812 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 679 812 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 679 812 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 679 812 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 679 812 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 679 812 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 679 812 NO: 724)
[2877] Segment cluster M78076_PEA.sub.--1_node.sub.--18 (SEQ ID
NO:728) according to the present invention is supported by 95
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 32 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01230 TABLE
32 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 813
991 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 813 991 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 813 991 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 813 991 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 813 991 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 813 991 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 813 991 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 813 991 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 813 991 NO: 724)
[2878] Segment cluster M78076_PEA.sub.--1_node.sub.--20 (SEQ ID
NO:729) according to the present invention is supported by 99
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 33 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01231 TABLE
33 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 992
1122 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 992 1122 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 992 1122 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 992 1122 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 992 1122 NO: 720) M78076_PEA.sub.--1
T23 (SEQ ID 992 1122 NO: 721) M78076_PEA.sub.--1_T26 (SEQ ID. 992
1122 NO: 722) M78076_PEA.sub.--1_T27 (SEQ ID 992 1122 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 992 1122 NO: 724)
[2879] Segment cluster M78076_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:730) according to the present invention is supported by 105
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T26 (SEQ
ID NO:722), M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and
M78076_PEA.sub.--1_T28 (SEQ ID NO:724). Table 34 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01232 TABLE 34 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1198 1356 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 1198 1356 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1198 1356 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1198 1356 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1198 1356 NO: 720)
M78076_PEA.sub.--1_T26 (SEQ ID 1198 1356 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 1198 1356 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 1198 1356 NO: 724)
[2880] Segment cluster M78076_PEA.sub.--1_node.sub.--26 (SEQ ID
NO:731) according to the present invention is supported by 99
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 35 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01 233 TABLE
35 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
1357 1485 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 1357 1485 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1357 1485 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1357 1485 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1357 1485 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1198 1326 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 1357 1485 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 1357 1485 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 1357 1485 NO: 724)
[2881] Segment cluster M78076_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:732) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T27 (SEQ ID NO:723). Table 36
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01234 TABLE 36 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M78076_PEA.sub.--1_T27 (SEQ ID 1490 3132 NO:
723)
[2882] Segment cluster M78076_PEA.sub.--1_node.sub.--32 (SEQ ID
NO:733) according to the present invention is supported by 2
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T26 (SEQ ID NO:722) and
M78076_PEA.sub.--1.sub.T27 (SEQ ID NO:723). Table 37 below
describes the starting and ending position of this segment on each
transcript. TABLE-US-01235 TABLE 37 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T26 (SEQ ID 1586 2457 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 3233 4104 NO: 723)
[2883] Segment cluster M78076 PEA.sub.--1_node.sub.--35 (SEQ ID
NO:734) according to the present invention is supported by 4
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716) and
M78076_PEA.sub.--1_T5 (SEQ ID NO:718). Table 38 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-01236 TABLE 38 Segment location on transcripts Segment
Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1694 1952 NO: 716)
M78076_PEA.sub.--1_T5 (SEQ ID 1694 1952 NO: 718)
[2884] Segment cluster M78076_PEA.sub.--1_node.sub.--37 (SEQ ID
NO:735) according to the present invention is supported by 11
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T3 (SEQ ID NO:717) and
M78076_PEA.sub.--1_T5 (SEQ ID NO:718). Table 39 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-01237 TABLE 39 Segment location on transcripts Segment
Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T3 (SEQ ID 1718 2180 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1977 2439 NO: 718)
[2885] Segment cluster M78076_PEA.sub.--1_node.sub.--46 (SEQ ID
NO:736) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T15 (SEQ ID NO:720). Table 40
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01238 TABLE 40 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M78076_PEA.sub.--1_T15 (SEQ ID 1852 1972 NO:
720)
[2886] Segment cluster M78076_PEA.sub.--1_node.sub.--47 (SEQ ID
NO:737) according to the present invention is supported by 155
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718) M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 41 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01239 TABLE
41 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1 T2 (SEQ ID
2111 2254 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2327 2470 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2586 2729 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1781 1924 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1973 2116 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1693 1836 NO: 721)
[2887] Segment cluster M78076_PEA.sub.--1_node.sub.--54 (SEQ ID
NO:738) according to the present invention is supported by 133
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721) and M78076_PEA.sub.--1_T28 (SEQ ID NO:724). Table 42
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01240 TABLE 42 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M78076_PEA.sub.--1_T2 (SEQ ID 2412 2715 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 2628 2931 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2887 3190 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 2082 2385 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2274 2577 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1994 2297 NO: 721)
M78076_PEA.sub.--1_T28 (SEQ ID 1492 1795 NO: 724)
[2888] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[2889] Segment cluster M78076_PEA.sub.--1_node.sub.--1 (SEQ ID
NO:739) according to the present invention is supported by 47
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 43 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01241 TABLE
43 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 161
204 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 161 204 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 161 204 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 161 204 NO: 719) M78076_PEA.sub.--1
T15 (SEQ ID 161 204 NO: 720) M78076_PEA 1 T23 (SEQ ID 161 204 NO:
721) M78076_PEA.sub.--1_T26 (SEQ ID 161 204 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 161 204 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 161 204 NO: 724)
[2890] Segment cluster M78076_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:740) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 44 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01242 TABLE
44 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 205
224 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 205 224 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 205 224 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 205 224 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 205 224 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 205 224 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 205 224 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 205 224 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 205 224 NO: 724)
[2891] Segment cluster M78076_PEA.sub.--1node.sub.--3 (SEQ ID
NO:741) according to the present invention is supported by 52
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718) M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 45 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01243 TABLE
45 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 225
288 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 225 288 NO: 717)
M78076_PEA.sub.--1 T5 (SEQ ID 225 288 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 225 288 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 225 288 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 225 288 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 225 288 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 225 288 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 225 288 NO: 724)
[2892] Segment cluster M78076_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:742) according to the present invention is supported by 59
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 46 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01244 TABLE
46 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 289
370 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 289 370 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 289 370 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 289 370 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 289 370 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 289 370 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 289 370 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 289 370 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 289 370 NO: 724)
[2893] Segment cluster M78076_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:743) according to the present invention is supported by 64
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 47 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01 245 TABLE
47 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 371
432 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 371 432 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 371 432 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 371 432 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 371 432 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 371 432 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 371 432 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 371 432 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 371 432 NO: 724)
[2894] Segment cluster M78076_PEA.sub.--1_node.sub.--12 (SEQ ID
NO:744) according to the present invention is supported by 71
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 48 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01246 TABLE
48 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID 566
678 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 566 678 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 566 678 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 566 678 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 566 678 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 566 678 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 566 678 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 566 678 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 566 678 NO: 724)
[2895] Segment cluster M78076_PEA.sub.--1_node.sub.--22 (SEQ ID
NO:745) according to the present invention is supported by 92
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722),
M78076_PEA.sub.--1_T27 (SEQ ID NO:723) and M78076_PEA.sub.--1_T28
(SEQ ID NO:724). Table 49 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01247 TABLE
49 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
1123 1197 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 1123 1197 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1123 1197 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1123 1197 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1123 1197 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1123 1197 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 1123 1197 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 1123 1197 NO: 723)
M78076_PEA.sub.--1_T28 (SEQ ID 1123 1197 NO: 724)
[2896] Segment cluster M78076_PEA.sub.--1_node.sub.--27 (SEQ ID
NO:746) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T27 (SEQ ID NO:723).
Table 50 below describes the starting and ending position of this
segment on each transcript. TABLE-US-01248 TABLE 50 Segment
location on transcripts Segment Segment Transcript name starting
position ending position M78076_PEA.sub.--1_T27 (SEQ ID 1486 1489
NO: 723)
[2897] Segment cluster M78076_PEA.sub.--1_node.sub.--30 (SEQ ID
NO:747) according to the present invention is supported by 90
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722) and
M78076_PEA.sub.--1_T27 (SEQ ID NO:723). Table 51 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01249 TABLE 51 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1486 1557 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 1486 1557 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1486 1557 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1486 1557 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1486 1557 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1327 1398 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 1486 1557 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 3133 3204 NO: 723)
[2898] Segment cluster M78076_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:748) according to the present invention is supported by 89
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721), M78076_PEA.sub.--1_T26 (SEQ ID NO:722) and
M78076_PEA.sub.--1_T27 (SEQ ID NO:723). Table 52 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01250 TABLE 52 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1558 1585 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 1558 1585 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1558 1585 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1558 1585 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1558 1585 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1399 1426 NO: 721)
M78076_PEA.sub.--1_T26 (SEQ ID 1558 1585 NO: 722)
M78076_PEA.sub.--1_T27 (SEQ ID 3205 3232 NO: 723)
[2899] Segment cluster M78076_PEA.sub.--1_node.sub.--34 (SEQ ID
NO:749) according to the present invention is supported by 103
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 53 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01251 TABLE
53 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
1586 1693 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 1586 1693 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1586 1693 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1586 1693 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1586 1693 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1427 1534 NO: 721)
[2900] Segment cluster M78076_PEA.sub.--1_node.sub.--36 (SEQ ID
NO:750) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 54 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01252 TABLE
54 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
1953 1976 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 1694 1717 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 1953 1976 NO: 718) M78076_PEA.sub.--1
T13 (SEQ ID 1694 1717 NO: 719) M78076_PEA.sub.--1_T15 (SEQ ID 1694
1717 NO: 720) M78076_PEA.sub.--1_T23 (SEQ ID 1535 1558 NO: 721)
[2901] Segment cluster M78076_PEA.sub.--1_node.sub.--41 (SEQ ID
NO:751) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T3 (SEQ ID NO:717) and
M78076_PEA.sub.--1_T5 (SEQ ID NO:718). Table 55 below describes the
starting and ending position of this segment on each transcript.
TABLE-US-01253 TABLE 55 Segment location on transcripts Segment
Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T3 (SEQ ID 2181 2192 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2440 2451 NO: 718)
[2902] Segment cluster M78076_PEA.sub.--1_node.sub.--42 (SEQ ID
NO:752) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and
M78076_PEA.sub.--1_T23 (SEQ ID NO:721). Table 56 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01254 TABLE 56 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1977 1985 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 2193 2201 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2452 2460 NO: 718)
M78076_PEA.sub.--1_T15 (SEQ ID 1718 1726 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1559 1567 NO: 721)
[2903] Segment cluster M78076_PEA.sub.--1_node.sub.--43 (SEQ ID
NO:753) according to the present invention is supported by 110
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and
M78076_PEA.sub.--1_T23 (SEQ ID NO:721). Table 57 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01255 TABLE 57 Segment location on transcripts
Segment Segment Transcript name starting position ending position
M78076_PEA.sub.--1_T2 (SEQ ID 1986 2047 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 2202 2263 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2461 2522 NO: 718)
M78076_PEA.sub.--1_T15 (SEQ ID 1727 1788 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1568 1629 NO: 721)
[2904] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 58. TABLE-US-01256 TABLE
58 Oligonucleotides related to this segment Oligonucleotide name
Overexpressed in cancers Chip reference
M78076.sub.--0.sub.--7.sub.--0 (SEQ ID breast malignant tumors BRS
NO: 914)
[2905] Segment cluster M78076_PEA.sub.--1_node.sub.--45 (SEQ ID
NO:754) according to the present invention is supported by 132
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 59 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01257 TABLE
59 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
2048 2110 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2264 2326 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2523 2585 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1718 1780 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 1789 1851 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1630 1692 NO: 721)
[2906] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 60. TABLE-US-01258 TABLE
60 Oligonucleotides related to this segment Oligonucleotide name
Overexpressed in cancers Chip reference
M78076.sub.--0.sub.--7.sub.--0 (SEQ ID breast malignant tumors BRS
NO: 914)
[2907] Segment cluster M78076_PEA.sub.--1_node.sub.--49 (SEQ ID
NO:755) according to the present invention is supported by 129
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 61 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01259 TABLE
61 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
2255 2290 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2471 2506 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2730 2765 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1925 1960 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2117 2152 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1837 1872 NO: 721)
[2908] Segment cluster M78076_PEA.sub.--1_node.sub.--50 (SEQ ID
NO:756) according to the present invention is supported by 125
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 62 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01 260 TABLE
62 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
2291 2329 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2507 2545 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2766 2804 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 1961 1999 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2153 2191 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1873 1911 NO: 721)
[2909] Segment cluster M78076_PEA.sub.--1_node.sub.--51 (SEQ ID
NO:757) according to the present invention is supported by 123
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 63 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01261 TABLE
63 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
2330 2388 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2546 2604 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2805 2863 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 2000 2058 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2192 2250 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1912 1970 NO: 721)
[2910] Segment cluster M78076_PEA.sub.--1_node.sub.--52 (SEQ ID
NO:758) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720) and M78076_PEA.sub.--1_T23
(SEQ ID NO:721). Table 64 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01262 TABLE
64 Segment location on transcripts Segment Segment Transcript name
starting position ending position M78076_PEA.sub.--1_T2 (SEQ ID
2389 2405 NO: 716) M78076_PEA.sub.--1_T3 (SEQ ID 2605 2621 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2864 2880 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 2059 2075 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2251 2267 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1971 1987 NO: 721)
[2911] Segment cluster M78076_PEA.sub.--1_node.sub.--53 (SEQ ID
NO:759) according to the present invention can be found in the
following transcript(s): M78076_PEA.sub.--1_T2 (SEQ ID NO:716),
M78076_PEA.sub.--1_T3 (SEQ ID NO:717), M78076_PEA.sub.--1_T5 (SEQ
ID NO:718), M78076_PEA.sub.--1_T13 (SEQ ID NO:719),
M78076_PEA.sub.--1_T15 (SEQ ID NO:720), M78076_PEA.sub.--1_T23 (SEQ
ID NO:721) and M78076_PEA.sub.--1_T28 (SEQ ID NO:724). Table 65
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01263 TABLE 65 Segment location on
transcripts Segment Segment Transcript name starting position
ending position M78076_PEA.sub.--1_T2 (SEQ ID 2406 2411 NO: 716)
M78076_PEA.sub.--1_T3 (SEQ ID 2622 2627 NO: 717)
M78076_PEA.sub.--1_T5 (SEQ ID 2881 2886 NO: 718)
M78076_PEA.sub.--1_T13 (SEQ ID 2076 2081 NO: 719)
M78076_PEA.sub.--1_T15 (SEQ ID 2268 2273 NO: 720)
M78076_PEA.sub.--1_T23 (SEQ ID 1988 1993 NO: 721)
M78076_PEA.sub.--1_T28 (SEQ ID 1486 1491 NO: 724)
[2912] Variant protein alignment to the previously known
protein:
[2913] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2914] Sequence Documentation:
[2915] Alignment of: M78076_PEA.sub.--1_P3 (SEQ ID
NO:761).times.APP1_HUMAN (SEQ ID NO:760).
[2916] Alignment segment 1/1:
[2917] Quality: 5132.00 TABLE-US-01264 Quality: 5132.00 Escore: 0
Matching length: 517 Total length: 517 Matching Percent 100.00
Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00114 Alignment: TABLE-US-01265 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500 501
GGSSEDKGGLQPPDSKD 517 ||||||||||||||||| 501 GGSSEDKGGLQPPDSKD
517
[2918] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2919] Sequence Documentation:
[2920] Alignment of: M78076_PEA.sub.--1_P4 (SEQ ID
NO:762).times.APP1_HUMAN (SEQ ID NO:760).
[2921] Alignment segment 1/1: TABLE-US-01266 Quality: 5223.00
Escore: 0 Matching length: 526 Total length: 526 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00115 Alignment: TABLE-US-01267 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRRTLRHYQHVAAVDPEKAQQMRFQVH 450 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500 501
GGSSEDKGGLQPPDSKDDTPMTLPKG 526 |||||||||||||||||||||||| 501
GGSSEDKGGLQPPDSKDDTPMTLPKG 526
[2922] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2923] Sequence Documentation:
[2924] Alignment of: M78076_PEA.sub.-1P12 (SEQ ID
NO:763).times.APP1_HUMAN (SEQ ID NO:760).
[2925] Alignment segment 1/1: TABLE-US-01268 Quality: 5223.00
Escore: 0 Matching length: 526 Total length: 526 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00116 Alignment: TABLE-US-01269 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500 501
GGSSEDKGGLQPPDSKDDTPMTLPKG 526 |||||||||||||||||||||||||| 501
GGSSEDKGGLQPPDSKDDTPMTLPKG 526
[2926] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2927] Sequence Documentation:
[2928] Alignment of: M78076_PEA.sub.--1_P14 (SEQ ID
NO:764).times.APP1_HUMAN (SEQ ID NO:760).
[2929] Alignment segment 1/1: TABLE-US-01270 Quality: 5672.00
Escore: 0 Matching length: 575 Total length: 575 Matching Percent
99.48 Matching Percent Identity: 99.48 Similarity: Total Percent
Similarity: 99.48 Total Percent Identity: 99.48 Gaps: 0
TABLE-US-00117 Alignment: TABLE-US-01271 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500 501
GGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKV 550
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
GGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKV 550 551
NASVPRGFPFHSSEIQRDELVRGGT 575 |||||||||||||||||||| || 551
NASVPRGFPFHSSEIQRDELAPAGT 575
[2930] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2931] Sequence Documentation:
[2932] Alignment of: M78076_PEA.sub.--1_P21 (SEQ ID
NO:765).times.APP1_HUMAN (SEQ ID NO:760).
[2933] Alignment segment 1/1: TABLE-US-01272 Quality: 5822.00
Escore: 0 Matching length: 597 Total length: 650 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 91.85 Total Percent Identity: 91.85 Gaps: 1
TABLE-US-00118 Alignment: TABLE-US-01273 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NE................................................ 352
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 353
.....AERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 397
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 398
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 447
|||||||||||||||||||||||||||||||||||||||||||||||||| 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAP 500 448
GGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYEPKV 497
|||||||||||||||||||||||||||||||||||||||||||||||||| 501
GGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKV 550 498
NASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSM 547
|||||||||||||||||||||||||||||||||||||||||||||||||| 551
NASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSM 600 548
LLLRRKKPYGAISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP 597
|||||||||||||||||||||||||||||||||||||||||||||||||| 601
LLLRRKKPYGAISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP 650
[2934] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2935] Sequence Documentation:
[2936] Alignment of: M78076_PEA.sub.--1_P24 (SEQ ID
NO:766).times.APP1_HUMAN (SEQ ID NO:760).
[2937] Alignment segment 1/1: TABLE-US-01274 Quality: 4791.00
Escore: 0 Matching length: 485 Total length: 485 Matching Percent
99.79 Matching Percent Identity: 99.59 Similarity: Total Percent
Similarity: 99.79 Total Percent Identity: 99.59 Gaps: 0
TABLE-US-00119 Alignment: TABLE-US-01275 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAWGKVTPTPRPTDGVDIYFGM 300 301
PGETSEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGETSEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIRECL 485
|||||||||||||||||||||||||||||||:| | 451
THLQVIEERVNQSLGLLDQNPHLAQELRPQIQELL 485
[2938] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2939] Sequence Documentation:
[2940] Alignment of: M78076_PEA.sub.--1_P2 (SEQ ID
NO:767).times.APP1_HUMAN (SEQ ID NO:760).
[2941] Alignment segment 1/1: TABLE-US-01276 Quality: 4474.00
Escore: 0 Matching length: 454 Total length: 454 Matching Percent
99.56 Matching Percent Identity: 99.34 Similarity: Total Percent
Similarity: 99.56 Total Percent Identity: 99.34 Gaps: 0
TABLE-US-00120 Alignment: TABLE-US-01277 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQWPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVL 450
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVH 450 451 TSFQ 454
| :| 451 THLQ 454
[2942] Sequence name: APP1_HUMAN (SEQ ID NO:760)
[2943] Sequence Documentation:
[2944] Alignment of: M78076_PEA.sub.--1_P25 (SEQ ID
NO:768).times.APP1_HUMAN (SEQ ID NO:760).
[2945] Alignment segment 1/1: TABLE-US-01278 Quality: 4455.00
Escore: 0 Matching length: 448 Total length: 448 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 100.00 Total Percent Identity: 100.00 Gaps: 0
TABLE-US-00121 Alignment: TABLE-US-01279 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEA 50 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100
|||||||||||||||||||||||||||||||||||||||||||||||||| 51
PGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYP 100 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150
|||||||||||||||||||||||||||||||||||||||||||||||||| 101
ELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200
|||||||||||||||||||||||||||||||||||||||||||||||||| 151
LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSD 200 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250
|||||||||||||||||||||||||||||||||||||||||||||||||| 201
RFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFP 250 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDTYFGM 300
|||||||||||||||||||||||||||||||||||||||||||||||||| 251
QPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDTYFGM 300 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350
|||||||||||||||||||||||||||||||||||||||||||||||||| 301
PGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL 350 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400
|||||||||||||||||||||||||||||||||||||||||||||||||| 351
NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQ 400 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQ 448
|||||||||||||||||||||||||||||||||||||||||||||||||| 401
ADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQ 448
Description for Cluster HSMUC1A
[2946] Cluster HSMUC1A features 14 transcript(s) and 22 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-01280 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSMUC1A_PEA.sub.--1_T12 769 HSMUC1A_PEA.sub.--1_T26
770 HSMUC1A_PEA.sub.--1_T28 771 HSMUC1A_PEA.sub.--1_T29 772
HSMUC1A_PEA.sub.--1_T30 773 HSMUC1A_PEA.sub.--1_T31 774
HSMUC1A_PEA.sub.--1_T33 775 HSMUC1A_PEA.sub.--1_T34 776
HSMUC1A_PEA.sub.--1_T35 777 HSMUC1A_PEA.sub.--1_T36 778
HSMUC1A_PEA.sub.--1_T40 779 HSMUC1A_PEA.sub.--1_T42 780
HSMUC1A_PEA.sub.--1_T43 781 HSMUC1A_PEA.sub.--1_T47 782
[2947] TABLE-US-01281 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSMUC1A_PEA.sub.--1_node.sub.--0 783
HSMUC1A_PEA.sub.--1_node.sub.--14 784
HSMUC1A_PEA.sub.--1_node.sub.--24 785
HSMUC1A_PEA.sub.--1_node.sub.--29 786
HSMUC1A_PEA.sub.--1_node.sub.--35 787
HSMUC1A_PEA.sub.--1_node.sub.--38 788
HSMUC1A_PEA.sub.--1_node.sub.--3 789
HSMUC1A_PEA.sub.--1_node.sub.--4 790
HSMUC1A_PEA.sub.--1_node.sub.--5 791
HSMUC1A_PEA.sub.--1_node.sub.--6 792
HSMUC1A_PEA.sub.--1_node.sub.--7 793
HSMUC1A_PEA.sub.--1_node.sub.--17 794
HSMUC1A_PEA.sub.--1_node.sub.--18 795
HSMUC1A_PEA.sub.--1_node.sub.--20 796
HSMUC1A_PEA.sub.--1_node.sub.--21 797
HSMUC1A_PEA.sub.--1_node.sub.--23 798
HSMUC1A_PEA.sub.--1_node.sub.--26 799
HSMUC1A_PEA.sub.--1_node.sub.--27 800
HSMUC1A_PEA.sub.--1_node.sub.--31 801
HSMUC1A_PEA.sub.--1_node.sub.--34 802
HSMUC1A_PEA.sub.--1_node.sub.--36 803
HSMUC1A_PEA.sub.--1_node.sub.--37 804
[2948] TABLE-US-01282 TABLE 3 Proteins of interest Sequence ID
Corresponding Protein Name No. Transcript(s)
HSMUC1A_PEA.sub.--1_P25 806 HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770)
HSMUC1A_PEA.sub.--1_P29 807 HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--11P30 808 HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_P32 809 HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--11P36 810 HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_P39 811 HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781)
HSMUC1A_PEA.sub.--1_P45 812 HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_P49 813 HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:813)
(SEQ ID NO:769) HSMUC1A_PEA.sub.--1_P52 814 HSMUC1A_PEA.sub.--1_T30
(SEQ ID NO:773) HSMUC1A_PEA.sub.--1_P53 815 HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774) HSMUC1A_PEA.sub.--1_P56 816 HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780) HSMUC1A_PEA.sub.--1_P58 817 HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777) HSMUC1A_PEA.sub.--1_P59 818 HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771) HSMUC1A_PEA.sub.--1_P63 819 HSMUC1A_PEA.sub.--1_T47
(SEQ ID NO:782)
[2949] These sequences are variants of the known protein Mucin 1
precursor (SwissProt accession identifier MUC1_HUMAN; known also
according to the synonyms MUC-1; Polymorphic epithelial mucin; PEM;
PEMT; Episialin; Tumor-associated mucin; Carcinoma-associated
mucin; Tumor-associated epithelial membrane antigen; EMA; H23AG;
Peanut-reactive urinary mucin; PUM; Breast carcinoma-associated
antigen DF3; CD227 antigen), SEQ ID NO: 805, referred to herein as
the previously known protein.
[2950] Protein Mucin 1 precursor (SEQ ID NO:805) is known or
believed to have the following function(s): May play a role in
adhesive functions and in cell-cell interactions, metastasis and
signaling. May provide a protective layer on epithelial surfaces.
Direct or indirect interaction with actin cytoskeleton. Isoform 7
behaves as a receptor and binds the secreted isoform 5. The binding
induces the phosphorylation of the isoform 7, alters cellular
morphology and initiates cell signaling. Can bind to GRB2 adapter
protein. The sequence for protein Mucin 1 precursor (SEQ ID NO:805)
is given at the end of the application, as "Mucin 1 precursor (SEQ
ID NO:805) amino acid sequence". Known polymorphisms for this
sequence are as shown in Table 4. TABLE-US-01283 TABLE 4 Amino acid
mutations for Known Protein SNP position(s) on amino acid sequence
Comment 1116 D->E: NO EFFECT ON BINDING OF ISOFORM 7.1116
D->A: DRASTICALLY REDUCED BINDING OF ISOFORM 7.2 T->A 134
P->Q 154 P->Q 1021 S->T1117V->M1193Q->L 1231
K->T1251 A->T
[2951] Protein Mucin 1 precursor (SEQ ID NO:805) localization is
believed to be Type I membrane protein. Two secreted forms (5 and
9) are also produced.
[2952] The previously known protein also has the following
indication(s) and/or potential therapeutic use(s): Cancer, breast;
Cancer, lung, non-small cell; Cancer, ovarian; Cancer, prostate. It
has been investigated for clinical/therapeutic use in humans, for
example as a target for an antibody or small molecule, and/or as a
direct therapeutic; available information related to these
investigations is as follows. Potential pharmaceutically related or
therapeutically related activity or activities of the previously
known protein are as follows: CD8 agonist; DNA antagonist;
Immunostimulant; Interferon gamma agonist; MUC-1 inhibitor. A
therapeutic role for a protein represented by the cluster has been
predicted. The cluster was assigned this field because there was
information in the drug database or the public databases (e.g.,
described herein above) that this protein, or part thereof, is used
or can be used for a potential therapeutic indication: Anticancer;
Monoclonal antibody, murine; Immunotoxin; Immunostimulant;
Immunoconjugate.
[2953] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: actin binding,
which are annotation(s) related to Molecular Function; and
cytoskeleton; integral plasma membrane protein, which are
annotation(s) related to Cellular Component.
[2954] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[2955] Cluster HSMUC1A can be used as a diagnostic marker according
to overexpression of transcripts of this cluster in cancer.
Expression of such transcripts in normal tissues is also given
according to the previously described methods. The term "number" in
the left hand column of the table and the numbers on the y-axis of
FIG. 43 refer to weighted expression of ESTs in each category, as
"parts per million" (ratio of the expression of ESTs for a
particular cluster to the expression of all ESTs in that category,
according to parts per million).
[2956] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 43 and Table 5. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: a mixture of malignant tumors from
different tissues, breast malignant tumors, pancreas carcinoma and
prostate cancer. TABLE-US-01284 TABLE 5 Normal tissue distribution
Name of Tissue Number bladder 41 brain 2 colon 66 epithelial 96
general 36 head and neck 314 kidney 282 lung 200 breast 61 ovary 0
pancreas 12 prostate 24 stomach 296 Thyroid 0 uterus 122
[2957] TABLE-US-01285 TABLE 6 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 bladder 3.3e-01
4.5e-01 1.8e-02 2.4 8.9e-02 1.7 brain 3.0e-02 2.6e-02 1.2e-01 4.6
1.1e-01 3.9 colon 1.2e-01 2.4e-01 3.8e-01 1.6 5.9e-01 1.2
epithelial 5.4e-02 6.0e-01 7.3e-06 1.8 6.2e-02 1.1 general 6.5e-07
2.6e-03 4.0e-23 3.6 1.7e-12 2.3 head and neck 6.4e-01 7.2e-01 1 0.3
1 0.3 kidney 7.8e-01 8.1e-01 1 0.3 1 0.2 lung 7.6e-01 7.9e-01
6.7e-01 0.8 1 0.4 breast 8.2e-02 1.3e-01 4.1e-03 3.6 7.7e-02 2.0
ovary 3.0e-02 4.3e-02 6.9e-02 4.4 1.6e-01 3.2 pancreas 7.2e-02
1.4e-01 9.6e-07 5.4 1.5e-05 4.5 prostate 7.0e-01 6.0e-01 1.5e-02
1.4 6.9e-04 3.2 stomach 3.1e-01 7.1e-01 1.5e-01 0.4 4.6e-01 0.8
Thyroid 2.9e-01 2.9e-01 4.4e-01 2.0 4.4e-01 2.0 uterus 2.4e-01
6.5e-01 1.6e-01 1.0 7.0e-01 0.6
[2958] For this cluster, at least one oligonucleotide was found to
demonstrate overexpression of the cluster, although not of at least
one transcript/segment as listed below. Microarray (chip) data is
also available for this cluster as follows. Various
oligonucleotides were tested for being differentially expressed in
various disease conditions, particularly cancer, as previously
described. The following oligonucleotides were found to hit this
cluster but not other segments/transcripts below (in relation to
breast cancer), shown in Table 7. TABLE-US-01286 TABLE 7
Oligonucleotides related to this cluster Oligonucleotide
Overexpressed in Chip name cancers reference
HSMUC1A.sub.--0.sub.--0.sub.--11364 breast malignant BRS (SEQ ID
NO:916) tumors
[2959] As noted above, cluster HSMUC1A features 14 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Mucin 1 precursor (SEQ
ID NO:805). A description of each variant protein according to the
present invention is now provided.
[2960] Variant protein HSMUC1A_PEA.sub.--1_P25 (SEQ ID NO:806)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide.
[2961] Variant protein HSMUC1A_PEA.sub.--1_P25 (SEQ ID NO:806) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 8, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P25 (SEQ ID
NO:806) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01287
TABLE 8 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 90 S->N
Yes 91 D->N No 157 Y->No 187 S->G No
[2962] Variant protein HSMUC1A_PEA.sub.--1_P25 (SEQ ID NO:806) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T26
(SEQ ID NO:770), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770) is shown in bold; this
coding portion starts at position 507 and ends at position 1115.
The transcript also has the following SNPs as listed in Table 9
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSMUC1A_PEA.sub.--1_P25 (SEQ ID NO:806) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01288 TABLE 9 Nucleic
acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 572 A->G No 775 G->A Yes 777
G->A No 977 C->No 1065 A->G No 1073 C->T No 1079
C->T Yes 1124 C->T Yes 1177 C->T No 1197 C->T Yes 1303
G->No 1315 G->A Yes 1316 C->No 1316 C->T No 1405A->T
No
[2963] Variant protein HSMUC1A_PEA.sub.--1_P29 (SEQ ID NO:807)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2964] Variant protein HSMUC1A_PEA.sub.--1_P29 (SEQ ID NO:807) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T33
(SEQ ID NO:775), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775) is shown in bold; this
coding portion starts at position 507 and ends at position 953. The
transcript also has the following SNPs as listed in Table 10 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P29 (SEQ ID NO:807) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01289 TABLE 10 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 964 C->No 1052 A->G No
1060 C->T No 1066 C->T Yes 1111 C->T Yes 1164 C->T No
1184 C->T Yes 1290 G->No 1302 G->A Yes 1303 C->No 1303
C->T No 1392 A->T No
[2965] Variant protein HSMUC1A_PEA.sub.--1_P30 (SEQ ID NO:808)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide.
[2966] Variant protein HSMUC1A_PEA.sub.--1_P30 (SEQ ID NO:808) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 11, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P30 (SEQ ID
NO:808) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01290
TABLE 11 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 120
Y->No 150 S->G No
[2967] Variant protein HSMUC1A_PEA.sub.--1_P30 (SEQ ID NO:808) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T34
(SEQ ID NO:776), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776) is shown in bold; this
coding portion starts at position 507 and ends at position 1004.
The transcript also has the following SNPs as listed in Table 12
(given according to their position on the nucleotide sequence, with
the alternative nucleic acid listed; the last column indicates
whether the SNP is known or not; the presence of known SNPs in
variant protein HSMUC1A_PEA.sub.--1_P30 (SEQ ID NO:808) sequence
provides support for the deduced sequence of this variant protein
according to the present invention). TABLE-US-01291 TABLE 12
Nucleic acid SNPs SNP position on Alternative Previously nucleotide
sequence nucleic acid known SNP? 599 A->G No 866 C->No 954
A->G No 962 C->T No 968 C->T Yes 1013 C->T Yes 1066
C->T No 1086 C->T Yes 1192 G->No 1204 G->A Yes 1205
C->No 1205 C->T No 1294 A->T No
[2968] Variant protein HSMUC1A_PEA.sub.--1_P32 (SEQ ID NO:809)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide.
[2969] Variant protein HSMUC1A_PEA.sub.--1_P32 (SEQ ID NO:809) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 13, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P32 (SEQ ID
NO:809) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01292
TABLE 13 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 111
Y->No 141 S->G No
[2970] Variant protein HSMUC1A_PEA.sub.--1_P32 (SEQ ID NO:809) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T36
(SEQ ID NO:778), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778) is shown in bold; this
coding portion starts at position 507 and ends at position 977. The
transcript also has the following SNPs as listed in Table 14 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P32 (SEQ ID NO:809) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01293 TABLE 14 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 839 C->No 927 A->G No
935 C->T No 941 C->T Yes 986 C->T Yes 1039 C->T No 1059
C->T Yes 1165G->No 1177 G->A Yes 1178 C->No 1178
C->T No 1267 A->T No
[2971] Variant protein HSMUC1A_PEA.sub.--1_P36 (SEQ ID NO:810)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2972] Variant protein HSMUC1A_PEA.sub.--1_P36 (SEQ ID NO:810) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 15, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P36 (SEQ ID
NO:810) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01294
TABLE 15 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 113
Y->No 143 S->G No
[2973] Variant protein HSMUC1A_PEA.sub.--1_P36 (SEQ ID NO:810) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T40
(SEQ ID NO:779), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779) is shown in bold; this
coding portion starts at position 507 and ends at position 983. The
transcript also has the following SNPs as listed in Table 16 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P36 (SEQ ID NO:810) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01295 TABLE 16 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 599 A->G No 845 C->No 933 A->G No
941 C->T No 947 C->T Yes 992 C->T Yes 1045 C->T No 1065
C->T Yes 1171 G->No 1183 G->A Yes 1184 C->No 1184
C->T No 1273 A->T No
[2974] Variant protein HSMUC1A_PEA.sub.--1_P39 (SEQ ID NO:811)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2975] Variant protein HSMUC1A_PEA.sub.--1_P39 (SEQ ID NO:811) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 17, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P39 (SEQ ID
NO:811) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01296
TABLE 17 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 90 Y->No
120 S->G No
[2976] Variant protein HSMUC1A_PEA.sub.--1_P39 (SEQ ID NO:811) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T43
(SEQ ID NO:781), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) is shown in bold; this
coding portion starts at position 507 and ends at position 914. The
transcript also has the following SNPs as listed in Table 18 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P39 (SEQ ID NO:811) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01297 TABLE 18 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 599 A->G No 776 C->No 864 A->G No
872 C->T No 878 C->T Yes 923 C->T Yes 976 C->T No 996
C->T Yes 1102 G->No 1114 G->A Yes 1115 C->No 1115
C->T No 1204A->T No
[2977] Variant protein HSMUC1A_PEA.sub.--1_P45 (SEQ ID NO:812)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2978] Variant protein HSMUC1A_PEA.sub.--1_P45 (SEQ ID NO:812) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T29
(SEQ ID NO:772), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772) is shown in bold; this
coding portion starts at position 507 and ends at position 746. The
transcript also has the following SNPs as listed in Table 19 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P45 (SEQ ID NO:812) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01298 TABLE 19 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 599 A->G No 746 G->A Yes 748 G->A
No 948 C->No 1036 A->G No 1044 C->T No 1050 C->T Yes
1095 C->T Yes 1148 C->T No 1168 C->T Yes 1274 G->No
1286 G->A Yes 1287 C->No 1287 C->T No 1376A->T No
[2979] Variant protein HSMUC1A_PEA.sub.--1_P49 (SEQ ID NO:813)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2980] Variant protein HSMUC1A_PEA.sub.--1_P49 (SEQ ID NO:813) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T12
(SEQ ID NO:769), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769) is shown in bold; this
coding portion starts at position 507 and ends at position 884. The
transcript also has the following SNPs as listed in Table 20 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P49 (SEQ ID NO:813) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01299 TABLE 20 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 704 G->A Yes 1012 G->A
Yes 1088 G->A Yes 1090 G->A No 1290 C->No 1378 A->G No
1386 C->T No 1392 C->T Yes 1437 C->T Yes 1490 C->T No
1510 C->T Yes 1616 G->No 1628 G->A Yes 1629 C->No 1629
C->T No 1718 A->T No
[2981] Variant protein HSMUC1A_PEA.sub.--1_P52 (SEQ ID NO:814)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to was secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2982] Variant protein HSMUC1A_PEA.sub.--1_P52 (SEQ ID NO:814) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T30
(SEQ ID NO:773), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773) is shown in bold; this
coding portion starts at position 507 and ends at position 719. The
transcript also has the following SNPs as listed in Table 21 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P52 (SEQ ID NO:814) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01300 TABLE 21 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 719 G->A Yes 721 G->A
No 921 C->No 1009 A->G No 1017 C->T No 1023 C->T Yes
1068 C->T Yes 1121 C->T No 1141 C->T Yes 1247 G->No
1259 G->A Yes 1260 C->No 1260 C->T No 1349A->T No
[2983] Variant protein HSMUC1A_PEA.sub.--1_P53 (SEQ ID NO:815)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T31 (SEQ ID NO:774). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2984] Variant protein HSMUC1A_PEA.sub.--1_P53 (SEQ ID NO:815) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T31 (SEQ ID NO:774) is shown in bold; this
coding portion starts at position 507 and ends at position 665. The
transcript also has the following SNPs as listed in Table 22 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P53 (SEQ ID NO:815) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01301 TABLE 22 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 669 G->A Yes 671 G->A
No 871 C->No 959A->G No 967C->T No 973C->T Yes
1018C->T Yes 1071 C->T No 1091 C->T Yes 1197 G->No 1209
G->A Yes 1210 C->No 1210 C->T No 1299A->T No
[2985] Variant protein HSMUC1A_PEA.sub.--1_P56 (SEQ ID NO:816)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T42 (SEQ ID NO:780). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2986] Variant protein HSMUC1A_PEA.sub.--1_P56 (SEQ ID NO:816) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 23, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P56 (SEQ ID
NO:816) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01302
TABLE 23 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 117
P->No
[2987] Variant protein HSMUC1A_PEA.sub.--1_P56 (SEQ ID NO:816) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T42 (SEQ ID NO:780) is shown in bold; this
coding portion starts at position 507 and ends at position 890. The
transcript also has the following SNPs as listed in Table 24 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P56 (SEQ ID NO:816) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01303 TABLE 24 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 855 C->No 943 A->G No
951 C->T No 957 C->T Yes 1002 C->T Yes 1055 C->T No
1075 C->T Yes 1181 G->No 1193 G->A Yes 1194 C->No 1194
C->T No 1283 A->T No
[2988] Variant protein HSMUC1A_PEA.sub.--1_P58 (SEQ ID NO:817)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T35 (SEQ ID NO:777). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2989] Variant protein HSMUC1A_PEA.sub.--1_P58 (SEQ ID NO:817) also
has the following non-silent SNPs (Single Nucleotide Polymorphisms)
as listed in Table 25, (given according to their position(s) on the
amino acid sequence, with the alternative amino acid(s) listed; the
last column indicates whether the SNP is known or not; the presence
of known SNPs in variant protein HSMUC1A_PEA.sub.--1_P58 (SEQ ID
NO:817) sequence provides support for the deduced sequence of this
variant protein according to the present invention). TABLE-US-01304
TABLE 25 Amino acid mutations SNP position(s) on Alternative
Previously amino acid sequence amino acid(s) known SNP? 147
P->No
[2990] Variant protein HSMUC1A_PEA.sub.--1_P58 (SEQ ID NO:817) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T35 (SEQ ID NO:777) is shown in bold; this
coding portion starts at position 507 and ends at position 980. The
transcript also has the following SNPs as listed in Table 26 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P58 (SEQ ID NO:817) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01305 TABLE 26 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 945 C->No 1033 A->G No
1041 C->T No 1047 C->T Yes 1092 C->T Yes 1145 C->T No
1165 C->T Yes 1271 G->No 1283 G->A Yes 1284 C->No 1284
C->T No 1373 A->T No
[2991] Variant protein HSMUC1A_PEA.sub.--1_P59 (SEQ ID NO:818)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T28 (SEQ ID NO:771). The location of the
variant protein was determined according to results from a number
of different software programs and analyses, including analyses
from SignalP and other specialized programs. The variant protein is
believed to be located as follows with regard to the cell:
secreted. The protein localization is believed to be secreted
because both signal-peptide prediction programs predict that this
protein has a signal peptide, and neither trans-membrane region
prediction program predicts that this protein has a trans-membrane
region.
[2992] Variant protein HSMUC1A_PEA.sub.--1_P59 (SEQ ID NO:818) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T28 (SEQ ID NO:771) is shown in bold; this
coding portion starts at position 507 and ends at position 794. The
transcript also has the following SNPs as listed in Table 27 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P59 (SEQ ID NO:818) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01306 TABLE 27 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 794 G->A Yes 796 G->A
No 996 C->No 1084 A->G No 1092 C->T No 1098 C->T Yes
1143 C->T Yes 1196 C->T No 1216 C->T Yes 1322 G->No
1334 G->A Yes 1335 C->No 1335 C->T No 1424A->T No
[2993] Variant protein HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). An alignment is given to
the known protein (Mucin 1 precursor (SEQ ID NO:805)) at the end of
the application. One or more alignments to one or more previously
published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[2994] Comparison report between HSMUC1A_PEA.sub.--1_P63 (SEQ ID
NO:819) and MUC1_HUMAN (SEQ ID NO:805):
[2995] 1.An isolated chimeric polypeptide encoding for
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819), comprising a first amino
acid sequence being at least 90% homologous to
MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSV corresponding to
amino acids 1-45 of MUC1_HUMAN (SEQ ID NO:805), which also
corresponds to amino acids 1-45 of HSMUC1A_PEA.sub.--1_P63 (SEQ ID
NO:819), and a second amino acid sequence being at least 70%,
optionally at least 80%, preferably at least 85%, more preferably
at least 90% and most preferably at least 95% homologous to a
polypeptide having the sequence
EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID NO:946)
corresponding to amino acids 46-85 of HSMUC1A_PEA.sub.--1_P63 (SEQ
ID NO:819), wherein said first amino acid sequence and second amino
acid sequence are contiguous and in a sequential order.
[2996] 2. An isolated polypeptide encoding for a tail of
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819), comprising a polypeptide
being at least 70%, optionally at least about 80%, preferably at
least about 85%, more preferably at least about 90% and most
preferably at least about 95% homologous to the sequence
TABLE-US-01307 EEEVSADQVSVGASGVLGSFKEARNAPSFLSWSFSMGPSK (SEQ ID
NO:946) in HSMUC1A_PEA.sub.--1_P63. (SEQ ID NO:819)
[2997] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[2998] The glycosylation sites of variant protein
HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819), as compared to the known
protein Mucin 1 precursor (SEQ ID NO:805), are described in Table
28 (given according to their position(s) on the amino acid sequence
in the first column; the second column indicates whether the
glycosylation site is present in the variant protein; and the last
column indicates whether the position is different on the variant
protein). TABLE-US-01308 TABLE 28 Glycosylation site(s) Position(s)
on known Present in amino acid sequence variant protein? 1055 no
957 no 975 no 1133 no 1029 no
[2999] Variant protein HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819) is
encoded by the following transcript(s): HSMUC1A_PEA.sub.--1_T47
(SEQ ID NO:782), for which the sequence(s) is/are given at the end
of the application. The coding portion of transcript
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782) is shown in bold; this
coding portion starts at position 507 and ends at position 761. The
transcript also has the following SNPs as listed in Table 29 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSMUC1A_PEA.sub.--1_P63 (SEQ ID NO:819) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01309 TABLE 29 Nucleic acid
SNPs SNP position on Alternative Previously nucleotide sequence
nucleic acid known SNP? 572 A->G No 900 A->No 904 C->No
963A->C Yes 1211 A->G No 1219 C->T No 1225 C->T Yes
1270 C->T Yes 1323 C->T No 1343 C->T Yes 1449 G->No
1461 G->A Yes 1462 C->No 1462 C->T No 1551 A->T No
[3000] As noted above, cluster HSMUC1A features 22 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[3001] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:783) according to the present invention is supported by 31
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 30 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01310 TABLE 30 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 1 564 (SEQ ID NO:769)
HSMUC1A_PEA.sub.--1_T26 1 564 (SEQ ID NO:770)
HSMUC1A_PEA.sub.--1_T28 1 564 (SEQ ID NO:771)
HSMUC1A_PEA.sub.--1_T29 1 564 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T30 1 564 (SEQ ID NO:773)
HSMUC1A_PEA.sub.--1_T31 1 564 (SEQ ID NO:774)
HSMUC1A_PEA.sub.--1_T33 1 564 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--1_T34 1 564 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_T35 1 564 (SEQ ID NO:777)
HSMUC1A_PEA.sub.--1_T36 1 564 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--1_T40 1 564 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_T42 1 564 (SEQ ID NO:780)
HSMUC1A_PEA.sub.--1_T43 1 564 (SEQ ID NO:781)
HSMUC1A_PEA.sub.--1_T47 1 564 (SEQ ID NO:782)
[3002] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--14 (SEQ ID
NO:784) according to the present invention is supported by 55
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769). Table 31
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01311 TABLE 31 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 666 841 (SEQ ID
NO:769)
[3003] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--24 (SEQ ID
NO:785) according to the present invention is supported by 135
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769). Table 32
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01312 TABLE 32 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 953 1084 (SEQ ID
NO:769)
[3004] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--29 (SEQ ID
NO:786) according to the present invention is supported by 156
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780) and HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781). Table
33 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01313 TABLE 33 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 1207 1346 (SEQ ID NO:769)
HSMUC1A_PEA.sub.--1_T26 894 1033 (SEQ ID NO:770)
HSMUC1A_PEA.sub.--1_T28 913 1052 (SEQ ID NO:771)
HSMUC1A_PEA.sub.--1_T29 865 1004 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T30 838 977 (SEQ ID NO:773)
HSMUC1A_PEA.sub.--1_T31 788 927 (SEQ ID NO:774)
HSMUC1A_PEA.sub.--1_T33 881 1020 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--1_T34 783 922 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_T35 862 1001 (SEQ ID NO:777)
HSMUC1A_PEA.sub.--1_T36 756 895 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--1_T40 762 901 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_T42 772 911 (SEQ ID NO:780)
HSMUC1A_PEA.sub.--1_T43 693 832 (SEQ ID NO:781)
[3005] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--35 (SEQ ID
NO:787) according to the present invention is supported by 51
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 34
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01314 TABLE 34 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T47 666 1189 (SEQ ID
NO:782)
[3006] Microarray (chip) data is also available for this segment as
follows. As described above with regard to the cluster itself,
various oligonucleotides were tested for being differentially
expressed in various disease conditions, particularly cancer. The
following oligonucleotides were found to hit this segment (in
relation to breast cancer), shown in Table 35. TABLE-US-01315 TABLE
35 Oligonucleotides related to this segment Oligonucleotide
Overexpressed in Chip name cancers reference
HSMUC1A.sub.--0.sub.--0.sub.--11365 breast malignant BRS (SEQ ID
NO:917) tumors
[3007] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--38 (SEQ ID
NO:788) according to the present invention is supported by 140
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 36 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01316 TABLE 36 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 1488 1749 (SEQ ID NO:769)
HSMUC1A_PEA.sub.--1_T26 1175 1436 (SEQ ID NO:770)
HSMUC1A_PEA.sub.--1_T28 1194 1455 (SEQ ID NO:771)
HSMUC1A_PEA.sub.--1_T29 1146 1407 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T30 1119 1380 (SEQ ID NO:773)
HSMUC1A_PEA.sub.--1_T31 1069 1330 (SEQ ID NO:774)
HSMUC1A_PEA.sub.--1_T33 1162 1423 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--1_T34 1064 1325 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_T35 1143 1404 (SEQ ID NO:777)
HSMUC1A_PEA.sub.--1_T36 1037 1298 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--1_T40 1043 1304 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_T42 1053 1314 (SEQ ID NO:780)
HSMUC1A_PEA.sub.--1_T43 974 1235 (SEQ ID NO:781)
HSMUC1A_PEA.sub.--1_T47 1321 1582 (SEQ ID NO:782)
[3008] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[3009] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--3 (SEQ ID
NO:789) according to the present invention is supported by 17
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T40
(SEQ ID NO:779) and HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781). Table
37 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01317 TABLE 37 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T29 565 591 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T34 565 591 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_T40 565 591 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_T43 565 591 (SEQ ID NO:781)
[3010] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:790) according to the present invention can be found in the
following transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 38 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01318 TABLE 38 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 565 573 (SEQ ID NO:769)
HSMUC1A_PEA.sub.--1_T26 565 573 (SEQ ID NO:770)
HSMUC1A_PEA.sub.--1_T28 565 573 (SEQ ID NO:771)
HSMUC1A_PEA.sub.--1_T29 592 600 (SEQ ID NO:772)
HSMUC1A_PEA.sub.--1_T30 565 573 (SEQ ID NO:773)
HSMUC1A_PEA.sub.--1_T31 565 573 (SEQ ID NO:774)
HSMUC1A_PEA.sub.--1_T33 565 573 (SEQ ID NO:775)
HSMUC1A_PEA.sub.--1_T34 592 600 (SEQ ID NO:776)
HSMUC1A_PEA.sub.--1_T35 565 573 (SEQ ID NO:777)
HSMUC1A_PEA.sub.--1_T36 565 573 (SEQ ID NO:778)
HSMUC1A_PEA.sub.--1_T40 592 600 (SEQ ID NO:779)
HSMUC1A_PEA.sub.--1_T42 565 573 (SEQ ID NO:780)
HSMUC1A_PEA.sub.--1_T43 592 600 (SEQ ID NO:781)
HSMUC1A_PEA.sub.--1_T47 565 573 (SEQ ID NO:782)
[3011] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--5 (SEQ ID
NO:791) according to the present invention is supported by 34
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1 T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 39 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01319 TABLE 39 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 574 600 (SEQ ID NO: 769)
HSMUC1A_PEA.sub.--1_T26 574 600 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 574 600 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 601 627 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 574 600 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 574 600 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 574 600 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 601 627 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 574 600 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 574 600 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 601 627 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 574 600 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 601 627 (SEQ ID NO: 781)
HSMUC1A_PEA.sub.--1_T47 574 600 (SEQ ID NO: 782)
[3012] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--6 (SEQ ID
NO:792) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 40 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01320 TABLE 40 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 601 638 (SEQ ID NO: 769)
HSMUC1A_PEA.sub.--1_T26 601 638 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 601 638 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 628 665 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 601 638 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 601 638 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 601 638 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 628 665 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 601 638 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 601 638 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 628 665 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 601 638 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 628 665 (SEQ ID NO: 781)
HSMUC1A_PEA.sub.--1_T47 601 638 (SEQ ID NO: 782)
[3013] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:793) according to the present invention is supported by 32
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780) and HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781). Table
42 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01321 TABLE 42 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 639 665 (SEQ ID NO: 769)
HSMUC1A_PEA.sub.--1_T26 639 665 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 639 665 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 666 692 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 639 665 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 639 665 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 639 665 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 666 692 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 639 665 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 639 665 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 666 692 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 639 665 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 666 692 (SEQ ID NO: 781)
[3014] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--17 (SEQ ID
NO:794) according to the present invention can be found in the
following transcript(s): HSMUC1A_PEA.sub.--1_T28 (SEQ ID NO:771),
HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775) and HSMUC1A_PEA.sub.--1_T40
(SEQ ID NO:779). Table 44 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01322 TABLE
44 Segment location on transcripts Segment Segment starting ending
Transcript name position position HSMUC1A_PEA.sub.--1_T28 666 684
(SEQ ID NO: 771) HSMUC1A_PEA.sub.--1_T33 666 684 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T40 693 711 (SEQ ID NO: 779)
[3015] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--18 (SEQ ID
NO:795) according to the present invention is supported by 90
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T33
(SEQ ID NO:775), HSMUC1A_PEA.sub.--1_T35 (SEQ ID NO:777),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779) and HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780). Table 45 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01323 TABLE
45 Segment location on transcripts Segment Segment starting ending
Transcript name position position HSMUC1A_PEA.sub.--1_T12 842 891
(SEQ ID NO: 769) HSMUC1A_PEA.sub.--1_T26 666 715 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 685 734 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 693 742 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 666 715 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T33 685 734 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T35 666 715 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T40 712 761 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 666 715 (SEQ ID NO: 780)
[3016] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--20 (SEQ ID
NO:796) according to the present invention can be found in the
following transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T35 (SEQ ID NO:777) and HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780). Table 46 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01324 TABLE
46 Segment location on transcripts Segment Segment starting ending
Transcript name position position HSMUC1A_PEA.sub.--1_T12 892 900
(SEQ ID NO: 769) HSMUC1A_PEA.sub.--1_T26 716 724 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 735 743 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T33 735 743 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T35 716 724 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T42 716 724 (SEQ ID NO: 780)
[3017] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--21 (SEQ ID
NO:797) according to the present invention is supported by 97
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T35 (SEQ ID NO:777) and HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780). Table 47 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01325 TABLE
47 Segment location on transcripts Segment Segment starting ending
Transcript name position position HSMUC1A_PEA.sub.--1_T12 901 947
(SEQ ID NO: 769) HSMUC1A_PEA.sub.--1_T26 725 771 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 744 790 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T33 744 790 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T35 725 771 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T42 725 771 (SEQ ID NO: 780)
[3018] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--23 (SEQ ID
NO:798) according to the present invention can be found in the
following transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769).
Table 48 below describes the starting and ending position of this
segment on each transcript. TABLE-US-01326 TABLE 48 Segment
location on transcripts Segment Segment starting ending Transcript
name position position HSMUC1A_PEA.sub.--1_T12 948 952 (SEQ ID NO:
769)
[3019] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--26 (SEQ ID
NO:799) according to the present invention is supported by 129
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773) and HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774). Table 49 below describes the starting and ending
position of this segment on each transcript. TABLE-US-01327 TABLE
49 Segment location on transcripts Segment Segment starting ending
Transcript name position position HSMUC1A_PEA.sub.--1_T12 1085 1116
(SEQ ID NO: 769) HSMUC1A_PEA.sub.--1_T26 772 803 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 791 822 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 743 774 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 716 747 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 666 697 (SEQ ID NO: 774)
[3020] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--27 (SEQ ID
NO:800) according to the present invention is supported by 140
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777) and HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778). Table
50 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01 328 TABLE 50 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 1117 1206 (SEQ ID NO:
769) HSMUC1A_PEA.sub.--1_T26 804 893 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 823 912 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 775 864 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 748 837 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 698 787 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 791 880 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 693 782 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 772 861 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 666 755 (SEQ ID NO: 778)
[3021] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--31 (SEQ ID
NO:801) according to the present invention can be found in the
following transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780) and HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781). Table
51 below describes the starting and ending position of this segment
on each transcript. TABLE-US-01329 TABLE 51 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T12 1347 1356 (SEQ ID NO:
769) HSMUC1A_PEA.sub.--1_T26 1034 1043 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 1053 1062 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 1005 1014 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 978 987 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 928 937 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 1021 1030 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 923 932 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 1002 1011 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 896 905 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 902 911 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 912 921 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 833 842 (SEQ ID NO: 781)
[3022] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--34 (SEQ ID
NO:802) according to the present invention is supported by 24
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 52
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01330 TABLE 52 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSMUC1A_PEA.sub.--1_T47 639 665 (SEQ ID NO:
782)
[3023] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--36 (SEQ ID
NO:803) according to the present invention is supported by 135
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 53 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01331 TABLE 53 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 1357 1388 (SEQ ID NO: 769)
HSMUC1A_PEA.sub.--1_T26 1044 1075 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 1063 1094 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 1015 1046 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 988 1019 (SEQ ID NO: 773)
HSMUC1A_PEA.sub.--1_T31 938 969 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 1031 1062 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 933 964 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 1012 1043 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 906 937 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 912 943 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 922 953 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 843 874 (SEQ ID NO: 781)
HSMUC1A_PEA.sub.--1_T47 1190 1221 (SEQ ID NO: 782)
[3024] Segment cluster HSMUC1A_PEA.sub.--1_node.sub.--37 (SEQ ID
NO:804) according to the present invention is supported by 146
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSMUC1A_PEA.sub.--1_T12 (SEQ ID NO:769),
HSMUC1A_PEA.sub.--1_T26 (SEQ ID NO:770), HSMUC1A_PEA.sub.--1_T28
(SEQ ID NO:771), HSMUC1A_PEA.sub.--1_T29 (SEQ ID NO:772),
HSMUC1A_PEA.sub.--1_T30 (SEQ ID NO:773), HSMUC1A_PEA.sub.--1_T31
(SEQ ID NO:774), HSMUC1A_PEA.sub.--1_T33 (SEQ ID NO:775),
HSMUC1A_PEA.sub.--1_T34 (SEQ ID NO:776), HSMUC1A_PEA.sub.--1_T35
(SEQ ID NO:777), HSMUC1A_PEA.sub.--1_T36 (SEQ ID NO:778),
HSMUC1A_PEA.sub.--1_T40 (SEQ ID NO:779), HSMUC1A_PEA.sub.--1_T42
(SEQ ID NO:780), HSMUC1A_PEA.sub.--1_T43 (SEQ ID NO:781) and
HSMUC1A_PEA.sub.--1_T47 (SEQ ID NO:782). Table 54 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01332 TABLE 54 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSMUC1A_PEA.sub.--1_T12 1389 1487 (SEQ ID NO: 769)
HSMUC1A_PEA.sub.--1_T26 1076 1174 (SEQ ID NO: 770)
HSMUC1A_PEA.sub.--1_T28 1095 1193 (SEQ ID NO: 771)
HSMUC1A_PEA.sub.--1_T29 1047 1145 (SEQ ID NO: 772)
HSMUC1A_PEA.sub.--1_T30 1020 1118 (SEQ ID NO: 773)
HSMUCIA_PEA.sub.--1_T31 970 1068 (SEQ ID NO: 774)
HSMUC1A_PEA.sub.--1_T33 1063 1161 (SEQ ID NO: 775)
HSMUC1A_PEA.sub.--1_T34 965 1063 (SEQ ID NO: 776)
HSMUC1A_PEA.sub.--1_T35 1044 1142 (SEQ ID NO: 777)
HSMUC1A_PEA.sub.--1_T36 938 1036 (SEQ ID NO: 778)
HSMUC1A_PEA.sub.--1_T40 944 1042 (SEQ ID NO: 779)
HSMUC1A_PEA.sub.--1_T42 954 1052 (SEQ ID NO: 780)
HSMUC1A_PEA.sub.--1_T43 875 973 (SEQ ID NO: 781)
HSMUC1A_PEA.sub.--1_T47 1222 1320 (SEQ ID NO: 782)
[3025] Variant protein alignment to the previously known
protein:
[3026] Sequence name: MUC1_HUMAN (SEQ ID NO:805)
[3027] Sequence Documentation:
[3028] Alignment of: HSMUC1A_PEA.sub.--1_P63 (SEQ ID
NO:819).times.MUC1_HUMAN (SEQ ID NO:805).
[3029] Alignment segment 1/1: TABLE-US-01333 Quality: 429.00
Escore: 0 Matching length: 59 Total length: 59 Matching Percent
86.44 Matching Percent Identity: 81.36 Similarity: Total Percent
Similarity: 86.44 Total Percent Identity: 81.36 Gaps: 0
TABLE-US-00122 Alignment: TABLE-US-01334 . . . 1
MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSVEEEVS 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MTPGTQSPFFLLLLLTVLTVVTGSGHASSTPGGEKETSATQRSSVPSSTE 50 51 ADQVSVGAS
59 : ||: :| 51 KNAVSMTSS 59
Combined Expression of 8 Sequences (T10888seg1-17 (SEQ ID NO: 832),
HUMGR5E junc3-7 (SEQ ID NO: 857), HSSTROL3seg24 (SEQ ID NO: 869),
T94936 Seg 14 (SEQ ID NO: 861), Z21368 seg39 (SEQ ID NO: 844),
Z21368 junc17-21 (SEQ ID NO: 847), T59832 jun6-25-26 (SEQ ID NO:
854) and M85491seg24 (SEQ ID NO: 866)) in Normal and Cancerous
Breast Tissues
[3030] Expression of CEA6_HUMAN Carcinoembryonic antigen-related
cell adhesion molecule 6, GRP_HUMAN--gastrin-releasing peptide,
Stromelysin-3 precursor (EC 3.4.24.-) (Matrix metalloproteinase-11)
(MMP-11) (ST3) (SL-3), Homo sapiens breast cancer membrane protein
11 (BCMP11), SUL1_HUMAN, Ephrin type-B receptor 2 precursor (EC
2.7.1.112) (Tyrosine-protein kinase receptor EPH-3 and
gamma-interferon inducible lysosomal thiol reductase (GILT)
transcripts detectable by or according to T10888seg11-17 (SEQ ID
NO: 832), HUMGR5E junc3-7 (SEQ ID NO: 857), HSSTROL3seg24 (SEQ ID
NO: 869), T94936 Seq 14 (SEQ ID NO: 861), Z21368 seg39 (SEQ ID NO:
844), Z21368 junc17-21 (SEQ ID NO: 874), T58832 jun6-25-26 (SEQ ID
NO: 854) and M85491seg24 (SEQ ID NO: 866) amplicons and
T10888seg11-17F (SEQ ID NO: 830), T10888seg11-17R (SEQ ID NO: 831),
HUMGR5E junc3-7F (SEQ ID NO: 855), HUMGR5E junc3-7F (SEQ ID NO:
856), HSSTROL3seg24F (SEQ ID NO:867), HSSTROL3seg24R (SEQ ID NO:
868), T94936seg14F (SEQ ID NO: 859), T94936seg14R (SEQ ID NO: 860),
Z21368 seg39F (SEQ ID NO: 842), Z21368 seg39R (SEQ ID NO: 843),
Z21368junc17-21 F (SEQ ID NO: 845), Z21368junc17-21 R (SEQ ID NO:
846), T59832 jun6-25-26F (SEQ ID NO: 852), T59832jun6-25-26F (SEQ
ID NO: 853), M85491seg24F (SEQ ID NO: 864) and M85491seg24R (SEQ ID
NO: 865) primers was measured by real time PCR. In parallel the
expression of four housekeeping genes--PBGD (GenBank Accession No.
BC019323 (SEQ ID NO:926); amplicon--PBGD-amplicon (SEQ ID NO:929)),
HPRT1 (GenBank Accession No. NM.sub.--000194 (SEQ ID NO:930);
amplicon--HPRT1-amplicon (SEQ ID NO:933), G6PD (GenBank Accession
No. NM.sub.--000402 (SEQ ID NO:918); G6PD-amplicon (SEQ ID NO:921))
and SDHA (GenBank Accession No. NM.sub.--004168 (SEQ ID NO:922);
amplicon--SDHA-amplicon (SEQ ID NO:925)) was measured similarly.
For each RT sample, the expression of the above amplicons was
normalized to the geometric mean of the quantities of the
housekeeping genes. The normalized quantity of each RT sample of
each amplicon was then divided by the median of the quantities of
the normal post-mortem (PM) samples detected for the same amplicon
(Sample Nos. 56-60, 63-67 Table 1, "Tissue samples in testing
panel" above), to a value of fold up-regulation for each sample
relative to median of the normal PM samples.
[3031] FIGS. 44-47 are histograms showing differential expression
of the above-indicated transcripts in cancerous breast samples
relative to the normal samples, in different combinations. The
number and percentage of samples that exhibit at least 5 fold
differential of at least one of the sequences, out of the total
number of samples tested is indicated in the bottom.
[3032] As is evident from FIGS. 44-47, differential expression of
at least 5 fold in at least one of the sequences was found in 25
out of 28 adenocarcinoma samples in all different combinations.
[3033] Statistical analysis was applied to verify the significance
of these results, as described below. Threshold of 5 fold
differential expression of at least one of the amplicons was found
to differentiate between cancer and normal samples.
[3034] The above values demonstrate statistical significance of the
results.
Description for Cluster HSU33147
[3035] Cluster HSU33147 features 2 transcript(s) and 5 segment(s)
of interest, the names for which are given in Tables 1 and 2,
respectively, the sequences themselves are given at the end of the
application. The selected protein variants are given in table 3.
TABLE-US-01335 TABLE 1 Transcripts of interest Transcript Name
Sequence ID No. HSU33147_PEA.sub.--1_T1820 HSU33147_PEA.sub.--1_T2
821
[3036] TABLE-US-01336 TABLE 2 Segments of interest Segment Name
Sequence ID No. HSU33147_PEA.sub.--1_node.sub.--0 822
HSU33147_PEA.sub.--1_node.sub.--2 823
HSU33147_PEA.sub.--1_node.sub.--4 824
HSU33147_PEA.sub.--1_node.sub.--7 825
HSU33147_PEA.sub.--1_node.sub.--3 826
[3037] TABLE-US-01337 TABLE 3 Proteins of interest Sequence ID
Corresponding Protein Name No. Transcript(s)
HSU33147_PEA.sub.--1_P5 828 HSU33147_PEA.sub.--1_T1; (SEQ ID NO:
820) HSU33147_PEA.sub.--1_T2 (SEQ ID NO: 821)
[3038] These sequences are variants of the known protein
Mammaglobin A precursor (SwissProt accession identifier MGBA_HUMAN;
known also according to the synonyms Mammaglobin 1; Secretoglobin
family 2A member 2), SEQ ID NO: 827, referred to herein as the
previously known protein.
[3039] The sequence for protein Mammaglobin A precursor (SEQ ID
NO:827) is given at the end of the application, as "Mammaglobin A
precursor (SEQ ID NO:827) amino acid sequence".
[3040] It has been investigated for clinical/therapeutic use in
humans, for example as a target for an antibody or small molecule,
and/or as a direct therapeutic; available information related to
these investigations is as follows. Potential pharmaceutically
related or therapeutically related activity or activities of the
previously known protein are as follows: Immunostimulant. A
therapeutic role for a protein represented by the cluster has been
predicted. The cluster was assigned this field because there was
information in the drug database or the public databases (e.g.,
described herein above) that this protein, or part thereof, is used
or can be used for a potential therapeutic indication:
Anticancer.
[3041] The following GO Annotation(s) apply to the previously known
protein. The following annotation(s) were found: steroid binding,
which are annotation(s) related to Molecular Function.
[3042] The GO assignment relies on information from one or more of
the SwissProt/TremBI Protein knowledgebase, available from
expasy.ch/sprot/; or Locuslink, available from
ncbi.nlm.nih.gov/projects/LocusLink/.
[3043] Cluster HSU33147 can be used as a diagnostic marker
according to overexpression of transcripts of this cluster in
cancer. Expression of such transcripts in normal tissues is also
given according to the previously described methods. The term
"number" in the left hand column of the table and the numbers on
the y-axis of FIG. 48 refer to weighted expression of ESTs in each
category, as "parts per million" (ratio of the expression of ESTs
for a particular cluster to the expression of all ESTs in that
category, according to parts per million).
[3044] Overall, the following results were obtained as shown with
regard to the histograms in FIG. 48 and Table 4. This cluster is
overexpressed (at least at a minimum level) in the following
pathological conditions: a mixture of malignant tumors from
different tissues. TABLE-US-01338 TABLE 4 Normal tissue
distribution Name of Tissue Number epithelial 6 general 2 lung 0
breast 131
[3045] TABLE-US-01339 TABLE 5 P values and ratios for expression in
cancerous tissue Name of Tissue P1 P2 SP1 R3SP2 R4 epithelial
4.1e-02 6.4e-02 1.5e-12 2.6 2.2e-06 1.5 general 1.6e-02 1.1e-02
1.2e-22 4.4 7.2e-13 2.4 lung 1 6.3e-01 1 1.0 6.2e-01 1.6 breast
8.6e-02 1.1e-01 3.4e-07 1.7 2.6e-03 1.0
[3046] As noted above, cluster HSU33147 features 2 transcript(s),
which were listed in Table 1 above. These transcript(s) encode for
protein(s) which are variant(s) of protein Mammaglobin A precursor
(SEQ ID NO:827). A description of each variant protein according to
the present invention is now provided.
[3047] Variant protein HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828)
according to the present invention has an amino acid sequence as
given at the end of the application; it is encoded by transcript(s)
HSU33147_PEA.sub.--1_T1 (SEQ ID NO:820). An alignment is given to
the known protein (Mammaglobin A precursor (SEQ ID NO:827)) at the
end of the application. One or more alignments to one or more
previously published protein sequences are given at the end of the
application. A brief description of the relationship of the variant
protein according to the present invention to each such aligned
protein is as follows:
[3048] Comparison report between HSU33147_PEA.sub.--1_P5 (SEQ ID
NO:828) and MGBA_HUMAN (SEQ ID NO:827):
[3049] 1.An isolated chimeric polypeptide encoding for
HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828), comprising a first amino
acid sequence being at least 90% homologous to
MKLLMVLMLAALSQHCYAGSGCPLLENVISKTINPQVSKTEYKELLQEFIDDNATTNAI
DELKECFLNQTDETLSNVE corresponding to amino acids 1-78 of MGBA_HUMAN
(SEQ ID NO:827), which also corresponds to amino acids 1-78 of
HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828), and a second amino acid
sequence being at least 90% homologous to QLIYDSSLCDLF
corresponding to amino acids 82-93 of MGBA_HUMAN (SEQ ID NO:827)
which also corresponds to amino acids 79-90 of
HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828), wherein said first amino
acid sequence and second amino acid sequence are contiguous and in
a sequential order.
[3050] 2.An isolated chimeric polypeptide encoding for an edge
portion of HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828), comprising a
polypeptide having a length "n", wherein n is at least about 10
amino acids in length, optionally at least about 20 amino acids in
length, preferably at least about 30 amino acids in length, more
preferably at least about 40 amino acids in length and most
preferably at least about 50 amino acids in length, wherein at
least two amino acids comprise EQ, having a structure as follows: a
sequence starting from any of amino acid numbers 78-x to 78; and
ending at any of amino acid numbers 79+((n-2)-x), in which x varies
from 0 to n-2.
[3051] The location of the variant protein was determined according
to results from a number of different software programs and
analyses, including analyses from SignalP and other specialized
programs. The variant protein is believed to be located as follows
with regard to the cell: secreted. The protein localization is
believed to be secreted because both signal-peptide prediction
programs predict that this protein has a signal peptide, and
neither trans-membrane region prediction program predicts that this
protein has a trans-membrane region.
[3052] The glycosylation sites of variant protein
HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828), as compared to the known
protein Mammaglobin A precursor (SEQ ID NO:827), are described in
Table 6 (given according to their position(s) on the amino acid
sequence in the first column; the second column indicates whether
the glycosylation site is present in the variant protein; and the
last column indicates whether the position is different on the
variant protein). TABLE-US-01340 TABLE 6 Glycosylation site(s)
Position(s) on known amino Present in acid sequence variant
protein? Position in variant protein? 68 yes 68 53 yes 53
[3053] Variant protein HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828) is
encoded by the following transcript(s): HSU33147_PEA.sub.--1_T1
(SEQ ID NO:820), for which the sequencers) is/are given at the end
of the application. The coding portion of transcript
HSU33147_PEA.sub.--1_T1 (SEQ ID NO:820) is shown in bold; this
coding portion starts at position 72 and ends at position 341. The
transcript also has the following SNPs as listed in Table 7 (given
according to their position on the nucleotide sequence, with the
alternative nucleic acid listed; the last column indicates whether
the SNP is known or not; the presence of known SNPs in variant
protein HSU33147_PEA.sub.--1_P5 (SEQ ID NO:828) sequence provides
support for the deduced sequence of this variant protein according
to the present invention). TABLE-US-01341 TABLE 7 Nucleic acid SNPs
SNP position on Alternative Previously nucleotide sequence nucleic
acid known SNP? 84 A->C No 124 C->No 396 A->G No
[3054] As noted above, cluster HSU33147 features 5 segment(s),
which were listed in Table 2 above and for which the sequence(s)
are given at the end of the application. These segment(s) are
portions of nucleic acid sequence(s) which are described herein
separately because they are of particular interest. A description
of each segment according to the present invention is now
provided.
[3055] Segment cluster HSU33147_PEA.sub.--1_node.sub.--0 (SEQ ID
NO:822) according to the present invention is supported by 38
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSU33147_PEA.sub.--1_T1 (SEQ ID NO:820) and
HSU33147_PEA.sub.--1_T2 (SEQ ID NO:821). Table 8 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01342 TABLE 8 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSU33147_PEA.sub.--1_T11 126 (SEQ ID NO: 820)
HSU33147_PEA.sub.--1_T2 1 126 (SEQ ID NO: 821)
[3056] Segment cluster HSU33147_PEA.sub.--1_node.sub.--2 (SEQ ID
NO:823) according to the present invention is supported by 44
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSU33147_PEA.sub.--1_T1 (SEQ ID NO:820) and
HSU33147_PEA.sub.--1_T2 (SEQ ID NO:821). Table 9 below describes
the starting and ending position of this segment on each
transcript. TABLE-US-01343 TABLE 9 Segment location on transcripts
Segment Segment starting ending Transcript name position position
HSU33147_PEA.sub.--1_T1127 305 (SEQ ID NO: 820)
HSU33147_PEA.sub.--1_T2 127 305 (SEQ ID NO: 821)
[3057] Segment cluster HSU33147_PEA.sub.--1_node.sub.--4 (SEQ ID
NO:824) according to the present invention is supported by 3
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSU33147_PEA.sub.--1_T2 (SEQ ID NO:821). Table 10
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01344 TABLE 10 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSU33147_PEA.sub.--1_T2 315 907 (SEQ ID NO:
821)
[3058] Segment cluster HSU33147_PEA.sub.--1_node.sub.--7 (SEQ ID
NO:825) according to the present invention is supported by 35
libraries. The number of libraries was determined as previously
described. This segment can be found in the following
transcript(s): HSU33147_PEA.sub.--1_T1 (SEQ ID NO:820). Table 11
below describes the starting and ending position of this segment on
each transcript. TABLE-US-01345 TABLE 11 Segment location on
transcripts Segment Segment starting ending Transcript name
position position HSU33147_PEA.sub.--1_T1306 516 (SEQ ID NO:
820)
[3059] According to an optional embodiment of the present
invention, short segments related to the above cluster are also
provided. These segments are up to about 120 bp in length, and so
are included in a separate description.
[3060] Segment cluster HSU33147_PEA.sub.--1_node.sub.--3 (SEQ ID
NO:826) according to the present invention can be found in the
following transcript(s): HSU33147_PEA.sub.--1_T2 (SEQ ID NO:821)
Table 12 below describes the starting and ending position of this
segment on each transcript. TABLE-US-01346 TABLE 12 Segment
location on transcripts Segment Segment starting ending Transcript
name position position HSU33147_PEA.sub.--1_T2 306 314 (SEQ ID NO:
821)
[3061] Sequence name: MGBA_HUMAN (SEQ ID NO:827)
[3062] Sequence Documentation:
[3063] Alignment of: HSU33147_PEA.sub.--1_P5 (SEQ ID
NO:828).times.MGBA_HUMAN (SEQ ID NO:827).
[3064] Alignment segment 1/1: TABLE-US-01347 Quality: 776.00
Escore: 0 Matching length: 90 Total length: 93 Matching Percent
100.00 Matching Percent Identity: 100.00 Similarity: Total Percent
Similarity: 96.77 Total Percent Identity: 96.77 Gaps: 1
TABLE-US-00123 Alignment: TABLE-US-01348 . . . 1
MKLLMVLMLAALSQHCYAGSGCPLLENVISKTINPQVSKTEYKELLQEFI 50
|||||||||||||||||||||||||||||||||||||||||||||||||| 1
MKLLMVLMLAALSQHCYAGSGCPLLENVISKTINPQVSKTEYKELLQEFI 50 . . . 51
DDNATTNAIDELKECFLNQTDETLSNVE...QLIYDSSLCDLF 90
|||||||||||||||||||||||||||| |||||||||||| 51
DDNATTNAIDELKECFLNQTDETLSNVEVFMQLIYDSSLCDLF 93
[3065] It is appreciated that certain features of the invention,
which are, for clarity, described in the context of separate
embodiments, may also be provided in combination in a single
embodiment. Conversely, various features of the invention, which
are, for brevity, described in the context of a single embodiment,
may also be provided separately or in any suitable
subcombination.
[3066] Although the invention has been described in conjunction
with specific embodiments thereof, it is evident that many
alternatives, modifications and variations will be apparent to
those skilled in the art. Accordingly, it is intended to embrace
all such alternatives, modifications and variations that fall
within the spirit and broad scope of the appended claims. All
publications, patents and patent applications mentioned in this
specification are herein incorporated in their entirety by
reference into the specification, to the same extent as if each
individual publication, patent or patent application was
specifically and individually indicated to be incorporated herein
by reference. In addition, citation or identification of any
reference in this application shall not be construed as an
admission that such reference is available as prior art to the
present invention.
Sequence CWU 0 SQTB SEQUENCE LISTING The patent application
contains a lengthy "Sequence Listing" section. A copy of the
"Sequence Listing" is available in electronic form from the USPTO
web site
(http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20060183131A1).
An electronic copy of the "Sequence Listing" will also be available
from the USPTO upon request and payment of the fee set forth in 37
CFR 1.19(b)(3).
Sequence CWU 0 SQTB SEQUENCE LISTING The patent application
contains a lengthy "Sequence Listing" section. A copy of the
"Sequence Listing" is available in electronic form from the USPTO
web site
(http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20090286957A1).
An electronic copy of the "Sequence Listing" will also be available
from the USPTO upon request and payment of the fee set forth in 37
CFR 1.19(b)(3).
0 SQTB SEQUENCE LISTING The patent application contains a lengthy
"Sequence Listing" section. A copy of the "Sequence Listing" is
available in electronic form from the USPTO web site
(http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20090286957A1).
An electronic copy of the "Sequence Listing" will also be available
from the USPTO upon request and payment of the fee set forth in 37
CFR 1.19(b)(3).
* * * * *
References