Avian transgenesis using an ovalbumin nucleotide sequence

Abstract

The invention includes delivering to an avian a nucleic acid molecule which includes nucleotide sequence of an ovalbumin gene expression controlling region and a heterologous coding sequence operably linked to the gene expression controlling region wherein the heterologous coding sequence is expressed in a cell of the avian.

Description

[0001] This application is a Divisional of U.S. patent application Ser. No. 10/733,042, filed Dec. 11, 2003, the disclosure of which is incorporated in its entirety herein by reference, which claims the benefit of U.S. provisional patent applications, Ser. Nos. 60/462,953, filed Apr. 15, 2003; 60/465,015, filed Apr. 24, 2003; and 60/469,488 filed May 9, 2003, all of which are hereby incorporated by reference herein in their entireties.

FIELD OF THE INVENTION

[0002] The present invention relates generally to an isolated nucleic acid molecule comprising an avian ovalbumin transcriptional regulatory control region and linked matrix attachment regions. The invention further relates to recombinant nucleic acids and expression vectors, genetically transformed cells and transgenic avians that comprise an avian ovalbumin transcriptional regulatory region operably linked to a heterologous polypeptide-encoding nucleic acid insert. The present invention also relates to the expression and production of the polypeptide-encoding nucleic acid molecule under the control of the isolated avian ovalbumin transcriptional regulatory region.

BACKGROUND

[0003] Transgenic technology to convert animals into "bioreactor" for the production of specific proteins or other substances of pharmaceutical interest (Gordon et al., 1987, Biotechnology 5: 1183-1187; Wilmut et al., 1990, Theriogenology 33: 113-123) offers significant advantages over more conventional methods of protein production by gene expression.

[0004] Recombinant nucleic acid molecules have been engineered so that an expressed heterologous protein may be joined to a protein or peptide that allows secretion of the transgenic expression product into milk or urine, from which the protein may then be recovered. These procedures may require lactating animals, with the attendant costs of maintaining individual animals or herds of large species, such as cows, sheep, or goats.

[0005] Historically, transgenic animals have been produced almost exclusively by microinjection of the fertilized egg. The pronuclei of fertilized eggs are microinjected in vitro with foreign, i.e., xenogeneic or allogeneic, heterologous DNA or hybrid DNA molecules. The microinjected fertilized eggs are then transferred to the genital tract of a pseudopregnant female (e.g., Krimpenfort et al., U.S. Pat. No. 5,175,384).

[0006] One system that holds potential is the avian reproductive system. The production of an avian egg begins with formation of a large yolk in the ovary of the hen. The unfertilized oocyte or ovum is positioned on top of the yolk sac. After ovulation, the ovum passes into the infundibulum of the oviduct where it is fertilized if sperm are present, and then moves into the magnum of the oviduct, which is lined with tubular gland cells. These cells secrete the egg-white proteins, including ovalbumin, lysozyme, ovomucoid, conalbumin and ovomucin, into the lumen of the magnum where they are deposited onto the avian embryo and yolk.

[0007] The hen oviduct offers outstanding potential as a protein bioreactor because of the high levels of protein production, the promise of proper folding and post-translation modification of the target protein, the ease of product recovery, and the shorter developmental period of chickens compared to other potential animal species. The chicken ovalbumin gene is highly expressed in the tubular glands of the mature hen oviduct and is therefore a suitable candidate for an efficient promoter for heterologous protein production in transgenic birds. Efforts have been made to create transgenic chickens expressing heterologous proteins in the oviduct by means of microinjection of DNA (PCT Publication WO 97/47739).

[0008] Gene expression must be considered not only from the perspective of cis-regulatory elements associated with a gene, and their interactions with trans-acting elements, but also with regard to the genetic environment in which they are located. Chromosomal positioning effects result in variations in levels of transgene expression associated with different locations of the transgene within the recipient genome. An important factor governing the level of transgene expression is the chromatin structure around a transgene, and how it cooperates with the cis-regulatory elements. While the deletion of a cis-regulatory element from a transgenic lysozyme locus can be sufficient to reduce or eliminate positional independence of the level of gene expression, there is also evidence that positional independence conferred on a transgene requires the cotransfer of many kilobases of DNA other than just the protein encoding region and the immediate cis-transcriptional regulatory elements.

[0009] Scattered throughout the chicken genome, including the chicken ovalbumin locus, are short sequences that resemble features of Long Terminal Repeats (LTRs) of retrovirus. The function of these elements is unclear but most likely may help define the DNAse hypersensitive (DHS) regions of a gene locus (Stein et al., 1983, Proc. Natl. Acad. Sci. U.S.A. 80: 6485-6489). Thus, flanking various avian genes are matrix attachment regions (5' and 3' MARs), alternatively referred to as "scaffold attachment regions" or SARs. The outer boundaries of the chicken lysozyme locus, for example, have been defined by the MARs (Phi-Van et al., 1988, E.M.B.O.J. 7: 655-664; Phi-Van & Stratling., 1996, Biochem. 35: 10735-10742). Deletion of a 1.32 kb or a 1.45 kb region, each comprising half of a 5' MAR, reduces positional variation in the level of transgene expression (Phi-Van & Stratling, supra).

[0010] The 5' matrix attachment region (5' MAR), located about -11.7 kb upstream of the chicken lysozyme transcription start site, can increase the level of gene expression by limiting the chromosomal positional effects exerted against a transgene (Phi-Van et al., 1988, supra). At least one other MAR is located 3' downstream of the protein encoding region. Although MAR nucleic acid sequences are conserved, little cross-hybridization is seen, indicating significant overall sequence variation. However, MARs of different species can interact with the nucleomatrices of heterologous species, to the extent, for example, that the chicken lysozyme MAR can associate with the plant tobacco nucleomatrix as well as that of the chicken oviduct cells (Mlynarona et al., 1994, Cell 6: 417-426; von Kries et al., 1990, Nucleic Acids Res. 18: 3881-3885). The lysozyme promoter region of chicken is also active when transfected into mouse fibroblast cells and linked to a reporter gene such as the bacterial chloramphenicol acetyltransferase gene. In each case, the presence of a 5' MAR element increased positional independency of the level of transcription (Stief et al., 1989, Nature 341: 343-345; Sippel et al., pgs. 257-265 in Houdeline L. M. (ed), "Transgenic Animals: Generation and Use").

[0011] The ability to direct the insertion of a transgene into a site in the genome of an animal where the positional effect is limited offers predictability of results during the development of a desired transgenic animal, and increased yields of the expressed product. Sippel and Steif disclose, in U.S. Pat. No. 5,731,178, methods to increase the expression of genes introduced into eukaryotic cells by flanking a transcription unit with scaffold attachment elements, in particular the 5' MAR isolated from the chicken lysozyme gene. The transcription unit disclosed by Sippel and Steif was an artificial construct that combined only the -6.1 kb enhancer element and the proximal promoter element (base position -579 to +15) from the lysozyme gene. Other promoter associated elements were not included.

[0012] Although individual cis-transcriptional regulatory elements associated with the chicken ovalbumin gene have been isolated and sequenced, together with short regions of flanking DNA, the entire nucleic acid sequence comprising the 5' upstream region of the ovalbumin gene has not been determined and has not been employed as a functional promoter to allow expression of a heterologous transgene.

[0013] What are still needed, however, are efficient transcription promoters that allow expression of transgenes in avian cells but with reduced positional variation.

[0014] What is also still needed is a gene expression promoter cassette that will allow expression of a transgene in the oviduct cells of an avian and efficient gene expression regardless of the chromosomal location of the expression system.

SUMMARY OF THE INVENTION

[0015] Briefly described, the present invention relates to novel isolated and recombinant nucleic acid molecules that comprise an avian ovalbumin transcriptional regulatory region and at least one matrix attachment region element.

[0016] The isolated and recombinant nucleic acid molecules of the present invention, because of the presence of at least one matrix attachment region, are useful for reducing chromosomal positional effects on a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient cell. Isolating an approximately 195 kb region of the chicken genome that includes regions upstream of the ovalbumin locus ensures that cis-elements are also included that will allow gene expression in a tissue-specific manner. The ovalbumin promoter region of the present invention, therefore, will allow expression of an operably linked heterologous nucleic acid insert by a transfected avian cell such as, for example, a somatic cell.

[0017] The present invention provides a novel isolated nucleic acid molecule of approximately 195 kb of the chicken genome, and truncated variants thereof, comprising a region of about 135 kb that is 5' upstream, and an approximately 45 kb region that is 3' downstream, of the ovalbumin-encoding region of the gene locus. The novel isolated chicken nucleic acid sequence includes matrix attachment regions both 5' and 3' of the ovalbumin gene and an ovalbumin transcriptional regulatory region that includes CR1 repeat elements, a proximal ovalbumin promoter. Interspersed among the elements are stretches of nucleic acid that serve at least to organize the elements in an ordered array. The novel isolated chicken genomic region also includes the ovalbumin-encoding region with a plurality of introns dispersed therein.

[0018] The present invention further provides recombinant nucleic acid molecules for operably linking an avian ovalbumin transcriptional regulatory region to a heterologous nucleic acid molecule insert encoding a polypeptide to be expressed by a transfected or transgenic cell. The heterologous nucleic acid molecule may be placed in frame with a signal peptide sequence. Translation initiation may start with the signal peptide and continue through the nucleic acid molecule to produce an expressed polypeptide having the desired amino acid sequence.

[0019] The sequence of the expressed heterologous nucleic acid insert may be optimized for codon usage by a host cell using approaches well known in the art. For example, codon usage may be optimized for an avian such as a chicken. This could be determined from the codon usage of at least one, and preferably more than one, protein expressed in a chicken cell. For example, the codon usage may be determined from the nucleic acid sequences encoding the proteins ovalbumin, lysozyme, ovomucin and ovotransferrin of chicken.

[0020] The recombinant nucleic acid molecules of the present invention may further comprise a polyadenylation signal sequence that allows transcription directed by an ovalbumin transcriptional regulatory region to extend beyond the heterologous nucleic acid encoding a desired heterologous polypeptide and to comprise a 3' untranslated region and a polyadenylated tail. Any suitable functional polyadenylation signal sequence may be linked to the 3' end of the heterologous nucleic acid insert, including the SV40 polyadenylation signal sequence, bovine growth hormone adenylation sequence or the like.

[0021] The recombinant nucleic acid molecules of the present invention may also comprise a chicken ovalbumin 3' domain. The 3' domain can include a 3' untranslated region of the ovalbumin gene, a polyadenylation signal and at least one MAR that, in combined action with an MAR upstream of the ovalbumin transcriptional regulatory region, may reduce positional variation in gene expression in transgenic avians.

[0022] Yet another aspect of the present invention is expression vectors suitable for delivery to a recipient cell, preferably an avian cell. The expression vectors provided by the present invention may comprise an avian ovalbumin transcriptional regulatory region that can be operably linked to a nucleic acid insert encoding a polypeptide, and optionally a polyadenylation signal sequence. The expression vectors of the present invention further comprise at least one MAR element, and preferably two MARs that flank the ovalbumin transcriptional regulatory region and which can non-randomly direct the insertion of the expression vector into the genome of a recipient eukaryotic cell. The expression vector may further comprise a bacterial plasmid sequence, a viral nucleic acid sequence, or fragments or variants thereof that may allow for replication of the vector in a suitable host.

[0023] Another aspect of the present invention is methods of expressing a heterologous polypeptide in a eukaryotic cell by transfecting the cell with a recombinant nucleic molecule comprising an avian ovalbumin transcriptional regulatory region operably linked to a nucleic acid insert encoding a polypeptide desired to be expressed and, optionally, a polyadenylation signal sequence, and culturing the transfected cell under conditions suitable for expression of the heterologous polypeptide under the control of the avian ovalbumin transcriptional regulatory region.

[0024] Also within the scope of the present invention are recombinant cells, tissues and animals containing non-naturally occurring recombinant nucleic acid molecules according to the present invention as described above. In one embodiment of the present invention, the transformed cell is a chicken oviduct cell and the nucleic acid insert comprises the chicken ovalbumin transcriptional regulatory region, a nucleic acid insert encoding a human interferon .alpha.2b that is codon optimized for expression in an avian cell, and an SV40 polyadenylation sequence. In another embodiment of the present invention, the nucleic acid insert encodes the heavy and light chains of an antibody.

[0025] Additional objects and aspects of the present invention will become more apparent upon review of the detailed description set forth below when taken in conjunction with the accompanying figures, which are briefly described as follows.

BRIEF DESCRIPTION OF THE FIGURES

[0026] FIG. 1-1 to 1-75 illustrates the nucleic acid sequence SEQ ID NO: 1 of a region of the chicken genome that includes a chicken ovalbumin transcriptional regulatory region and the chicken ovalbumin gene, and matrix attachment regions 5' upstream and 3' downstream thereof.

[0027] FIG. 2 schematically illustrates the chicken genomic region having nucleic acid sequence SEQ ID NO: 1, indicating the relative positions and orientations of regions having identity with known domains.

[0028] FIG. 3 illustrates schematically the construction of an expression bacterial artificial chromosome where the insert gene of interest is under the expression control of the chicken ovalbumin promoter. Genes of interest may be inserted into the native translation start site of the ovalbumin gene. L and roman numerals, ovalbumin exons; GOI, gene of interest; start, translation start site; stop, translation stop site; pA, polyadenylation signal; E, EcoR1 site.

[0029] FIG. 4 illustrates an SV40 polyadenylation signal sequence SEQ ID NO: 2.

[0030] FIG. 5 illustrates the nucleotide sequence SEQ ID NO: 3 of a human interferon .alpha.2b interferon optimized for expression in an avian cell.

[0031] FIG. 6 illustrates the reconstruction of the chicken genomic region containing the ovalbumin locus.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0032] This description uses gene nomenclature accepted by the Cucurbit Genetics Cooperative as it appears in the Cucurbit Genetics Cooperative Report 18:85 (1995), which are incorporated herein by reference in its entirety. Using this gene nomenclature, genes are symbolized by italicized Roman letters. If a mutant gene is recessive to the normal type, then the symbol and name of the mutant gene appear in italicized lower case letters.

[0033] For convenience, definitions of certain terms employed in the specification, examples, and appended claims are collected here.

Definitions

[0034] The term "avian" as used herein refers to any species, subspecies or race of organism of the taxonomic class ava, such as, but not limited to chicken, turkey, duck, goose, quail, pheasants, parrots, finches, hawks, crows and ratites including ostrich, emu and cassowary. The term includes the various known strains of Gallus gallus, or chickens, (for example, White Leghorn, Brown Leghorn, Barred-Rock, Sussex, New Hampshire, Rhode Island, Ausstralorp, Minorca, Amrox, California Gray, Italian Partidge-colored), as well as strains of turkeys, pheasants, quails, duck, ostriches and other poultry commonly bred in commercial quantities. It also includes an individual avian organism in all stages of development, including embryonic and fetal stages. The term "avian" also may denote "pertaining to a bird", such as "an avian (bird) cell."

[0035] The term "nucleic acid" as used herein refers to any natural or synthetic linear and sequential array of nucleotides and nucleosides, for example cDNA, genomic DNA, mRNA, tRNA, oligonucleotides, oligonucleosides and derivatives thereof. For ease of discussion, such nucleic acids may be collectively referred to herein as "constructs," "plasmids," or "vectors." The term "nucleic acid" further includes modified or derivatized nucleotides and nucleosides such as, but not limited to, halogenated nucleotides such as, but not only, 5-bromouracil, and derivatised nucleotides such as biotin-labeled nucleotides.

[0036] The term "isolated nucleic acid molecule" as used herein refers to a nucleic acid molecule with a structure not identical to a naturally occurring nucleic acid molecule and includes DNA, RNA, or derivatives or variants thereof. The term covers, but is not limited to, (a) a DNA which has the sequence of part of a naturally occurring genomic molecule but is not flanked by at least one of the coding sequences that flank that part of the molecule in the genome of the species in which it naturally occurs; (b) a nucleic acid incorporated into a vector or into the genomic nucleic acid of a prokaryote or eukaryote in a manner such that the resulting molecule is not identical to any vector or naturally occurring genomic DNA; (c) a separate molecule such as a cDNA, a genomic fragment, a fragment produced by polymerase chain reaction (PCR), ligase chain reaction (LCR) or chemical synthesis, or a restriction fragment; (d) a recombinant nucleotide sequence that is part of a hybrid gene, i.e., a gene encoding a fusion protein, and (e) a recombinant nucleotide sequence that is part of a hybrid sequence that is not naturally occurring. Isolated nucleic acid molecules of the present invention can include, for example, natural allelic variants as well as nucleic acid molecules modified by nucleotide deletions, insertions, inversions, or substitutions such that the resulting nucleic acid molecule still essentially encodes an ovalbumin transcriptional regulatory region or a variant thereof of the present invention.

[0037] The terms "polynucleotide," "oligonucleotide," and "nucleic acid sequence" are used interchangeably herein and include, but are not limited to, coding sequences (polynucleotide(s) or nucleic acid sequence(s) which are transcribed and translated into polypeptide in vitro or in vivo when placed under the control of appropriate regulatory or control sequences); control sequences (e.g., translational start and stop codons, promoter sequences, ribosome binding sites, polyadenylation signals, transcription factor binding sites, transcription termination sequences, upstream and downstream regulatory domains, enhancers, silencers, and the like); and regulatory sequences (DNA sequences to which a transcription factor(s) binds and alters the activity of a gene's promoter either positively (induction) or negatively (repression)). No limitation as to length or to synthetic origin are suggested by the terms described above.

[0038] As used herein the terms "peptide," "polypeptide" and "protein" refer to a polymer of amino acids in a serial array, linked through peptide bonds. A "peptide" typically is a polymer of at least two to about 30 amino acids linked in a serial array by peptide bonds. The term "polypeptide" includes proteins, protein fragments, protein analogues, oligopeptides and the like. The term "polypeptides" contemplates polypeptides as defined above that are encoded by nucleic acids, produced through recombinant technology (isolated from an appropriate source such as a bird), or synthesized. The term "polypeptides" further contemplates polypeptides as defined above that include chemically modified amino acids or amino acids covalently or noncovalently linked to labeling moieties.

[0039] The term "fragment" as used herein refers to any isolated portion of the subject nucleic acid molecule constructed artificially (e.g., by chemical synthesis) or by cleaving a natural product into multiple pieces, using restriction endonucleases or mechanical shearing, or a portion of a nucleic acid synthesized by DNA polymerase, including by PCR, or any other polymerizing technique well known in the art, or expressed in a host cell by recombinant nucleic acid technology well known to one of skill in the art. The term "fragment" as used herein may also refer to an isolated portion of a polypeptide, wherein the portion of the polypeptide is cleaved from a naturally occurring polypeptide by proteolytic cleavage by at least one protease, or is a portion of the naturally occurring polypeptide synthesized by chemical or recombinant methods well known to one of skill in the art.

[0040] The terms "recombinant nucleic acid" and "recombinant DNA" as used herein refer to combinations of at least two nucleic acid sequences that are not naturally found in a eukaryotic or prokaryotic cell. The nucleic acid sequences may include, but are not limited to, nucleic acid vectors, gene expression regulatory elements, origins of replication, suitable gene sequences that when expressed confer antibiotic resistance, protein-encoding sequences and the like. The term "recombinant polypeptide" is meant to include a polypeptide produced by recombinant DNA techniques. A recombinant polypeptide may be distinct from a naturally occurring polypeptide either in its location, purity or structure. Generally, a recombinant polypeptide will be present in a cell in an amount different from that normally observed in nature.

[0041] The term "gene" or "genes" as used herein refers to nucleic acid sequences that encode genetic information for the synthesis of a whole RNA, a whole protein, or any portion of such whole RNA or whole protein. Genes that are not naturally part of a particular organism's genome are referred to as "foreign genes," "heterologous genes" or "exogenous genes" and genes that are naturally a part of a particular organism's genome are referred to as "endogenous genes". The term "gene product" refers to an RNA or protein that is encoded by the gene. "Endogenous gene products" are RNAs or proteins encoded by endogenous genes. "Heterologous gene products" are RNAs or proteins encoded by "foreign, heterologous or exogenous genes" and are, therefore, not naturally expressed in the cell.

[0042] The term "expressed" or "expression" as used herein refers to the transcription from a gene to give an RNA nucleic acid molecule at least complementary in part to a region of one of the two nucleic acid strands of the gene. The term "expressed" or "expression" as used herein may also refer to the translation from an RNA molecule to give a protein, a polypeptide or a portion thereof.

[0043] As used herein, the term "locus" refers to the site of a gene on a chromosome. In diploid organisms, pairs of genes control hereditary traits, each in the same position on a pair of chromosomes. These gene pairs, or alleles, may both be dominant or both be recessive in expression of that trait. In either case, the individual is said to be homozygous for the trait controlled by that gene pair. If the gene pair (alleles) consists of one dominant and one recessive trait, the individual is heterozygous for the trait controlled by the gene pair.

[0044] The term "operably linked" refers to an arrangement of elements wherein the components so described are configured so as to perform their usual function. Control sequences operably linked to a coding sequence are capable of effecting the expression of the coding sequence. The control sequences need not be contiguous with the coding sequence, so long as they function to direct the expression thereof. For example, intervening untranslated yet transcribed sequences can be present between a promoter sequence and the coding sequence and the promoter sequence can still be considered "operably linked" to the coding sequence.

[0045] The term "transcription regulatory sequences" as used herein refers to nucleotide sequences that are associated with a gene nucleic acid sequence and which regulate the transcriptional expression of the gene. Exemplary transcription regulatory sequences include enhancer elements, hormone response elements, steroid response elements, negative regulatory elements, and the like.

[0046] The term "promoter" as used herein refers to the DNA sequence that determines the site of transcription initiation by an RNA polymerase. A "promoter-proximal element" is a regulatory sequence generally within about 200 base pairs of the transcription start site.

[0047] The term "matrix attachment region" as used herein refers to a region of a eukaryotic genomic DNA that can be bound to chromosomal scaffold proteins. Matrix (scaffold) attachment regions (MARs) are generally located between transcription units such that the transcription units are within chromosomal loops. The bases of the loops are connected to the scaffold proteins through the MAR at each base. MARs and MAR-like homologs are identified as several recognizable nucleic acid sequences including, but not limited to, TG-rich spans, AT-rich regions and consensus sequences as described by Wang et al, J. Biol. Chem. 270:23239-23242 (1995). MARs may be identified by using suitable software such as, for example, MAR-WIZ.TM. (Futuresoft, Michigan, USA)

[0048] The term "internal ribosome entry sites (IRES)" as used herein refers to a region of a nucleic acid, most typically an RNA molecule, wherein eukaryotic initiation of protein synthesis occurs far downstream of the 5' end of the RNA molecule. A 43S pre-initiation complex comprising the elf2 protein bound to GTP and Met-tRNA.sub.i.sup.Met, the 40S ribosomal subunit, and faction elf3 and 31f1A may bind to an "IRES" before locating an AUG start codon. An "IRES" may be used to initiate translation of a second coding region downstream of a first coding region, wherein each coding region is expressed individually, but under the initial control of a single upstream promoter. An "IRES" may be located in a eukaryotic cellular mRNA.

[0049] The term "coding region" as used herein refers to a continuous linear arrangement of nucleotides which may be translated into a polypeptide. A full length coding region is translated into a full length protein; that is, a complete protein as would be translated in its natural state absent any post-translational modifications. A full length coding region may also include any leader protein sequence or any other region of the protein that may be excised naturally from the translated protein.

[0050] The terms "complementary", "complementarity" or "complement" as used herein refers to two nucleic acid molecules that can form specific interactions with one another to form a base-paired double helix.

[0051] The term "probe" as used herein, when referring to a nucleic acid, refers to a nucleotide sequence that can be used to anneal or hybridize with and thereby identify the presence of a complementary sequence, or a complementary sequence differing from the probe sequence but not to a degree that prevents hybridization under the hybridization stringency conditions used. The probe may be modified with labels such as, but not only, radioactive groups, biotin, and the like that are well known in the art.

[0052] The term "hybridizing under stringent conditions" as used herein refers to annealing a first nucleic acid to a second nucleic acid under stringent conditions as defined below. Stringent hybridization conditions typically permit the hybridization of nucleic acid molecules having at least 70% nucleic acid sequence complementarity with the nucleic acid molecule being used as a probe in the hybridization reaction, e.g., high temperature and/or low salt content that tend to disfavor hybridization of dissimilar nucleotide sequences. Alternatively, hybridization of the first and second nucleic acid may be conducted under reduced stringency conditions, e.g., low temperature and/or high salt content that tend to favor hybridization of dissimilar nucleotide sequences. Low stringency hybridization conditions may be followed by high stringency conditions or intermediate medium stringency conditions to increase the selectivity of the binding of the first and second nucleic acids. The hybridization conditions may further include reagents such as, but not limited to, dimethyl sulfoxide (DMSO) or formamide to disfavor still further the hybridization of dissimilar nucleotide sequences. A suitable hybridization protocol may, for example, involve hybridization in 6.times.SSC (wherein 1.times.SSC comprises 0.015 M sodium citrate and 0.15 M sodium chloride), at 65.degree. Celsius in an aqueous solution, followed by washing with 1.times.SSC at 65.degree. Celsius. Formulae to calculate appropriate hybridization and wash conditions to achieve hybridization permitting 30% or less mismatch between two nucleic acid molecules are disclosed, for example, in Meinkoth et al., 1984, Anal. Biochem. 138: 267-284; the content of which is incorporated herein by reference in its entirety. Protocols for hybridization techniques are well known to those of skill in the art and standard molecular biology manuals may be consulted to select a suitable hybridization protocol without undue experimentation. See, for example, Sambrook et al., 1989, "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor Press, the contents of which are herein incorporated by reference in its entirety.

[0053] Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) from about pH 7.0 to about pH 8.3 and the temperature is at least about 30.degree. Celsius for short probes (e.g., 10 to 50 nucleotides) and at least about 60.degree. Celcius for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37.degree. Celsius, and a wash in 1.times. to 2.times.SSC at 50 to 55.degree. Celsius. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 M NaCl, 1% SDS at 37.degree. Celsius, and a wash in 0.5.times. to 1.times.SSC at 55 to 60.degree. Celsius. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37.degree. Celsius, and a wash in 0.1.times.SSC at 60 to 65.degree. Celsius.

[0054] The terms "percent sequence identity" as used herein refers to the degree of sequence identity between two nucleic acid sequences or two amino acid sequences as determined using the algorithm of Karlin & Attschul, 1990, Proc. Natl. Acad. Sci. 87: 2264-2268, modified as in Karlin & Attschul, 1993, Proc. Natl. Acad. Sci. 90: 5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Attschul et al., 1990, J. Mol. Biol. Q15: 403-410. BLAST nucleotide searches are performed with the NBLAST program, score=100, wordlength=12, to obtain nucleotide sequences homologous to a nucleic acid molecule of the invention. BLAST protein searches are performed with the XBLAST program, score=50, wordlength=3, to obtain amino acid sequences homologous to a reference polypeptide. To obtain gapped alignments for comparison purposes, Gapped BLAST is utilized as described in Attschul et al., 1997, Nucl. Acids Res. 25: 3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g. XBLAST and NBLAST) are used. Other algorithms, programs and default settings may also be suitable such as, but not only, the GCG-Sequence Analysis Package of the U.K. Human Genome Mapping Project Resource Centre that includes programs for nucleotide or amino acid sequence comparisons.

[0055] The terms "vector" or "nucleic acid vector" as used herein refer to a natural or synthetic single or double stranded plasmid or viral nucleic acid molecule (RNA or DNA) that can be transfected or transformed into cells and replicate independently of, or within, the host cell genome. The term "expression vector" as used herein refers to a nucleic acid vector that comprises a transcription regulatory region operably linked to a site wherein is, or can be, inserted, a nucleotide sequence to be transcribed and, optionally, to be expressed, for instance, but not limited to, a sequence coding at least one polypeptide.

[0056] The term "transfection" as used herein refers to the process of inserting a nucleic acid into a host cell. Many techniques are well known to those skilled in the art to facilitate transfection of a nucleic acid into an eukaryotic cell. These methods include, for instance, treating the cells with high concentrations of salt such as a calcium or magnesium salt, an electric field, detergent, or liposome mediated transfection, to render the host cell competent for the uptake of the nucleic acid molecules, and by such methods as micro-injection into a pro-nucleus, sperm-mediated and restriction-mediated integration.

[0057] The terms "recombinant cell" and "genetically transformed cell" refer to a cell comprising a combination of nucleic acid segments not found in a single cell with each other in nature. A new combination of nucleic acid segments can be introduced into an organism using a wide array of nucleic acid manipulation techniques available to those skilled in the art. A recombinant cell can be a prokaryotic cell, or a eukaryotic cell, such as, but not limited to, an avian cell. The recombinant cell may harbor a vector that is extragenomic, i.e. that does not covalently insert into the cellular genome, including a non-nuclear (e.g. mitochondrial) genome(s). A recombinant cell may further harbor a vector or a portion thereof that is intragenomic, i.e. covalently incorporated within the genome (including non-nuclear genome(s)) of the recombinant cell.

[0058] As used herein, a "transgenic avian" is any avian, as defined above, including the chicken, in which one or more of the cells of the avian contain heterologous nucleic acid introduced by manipulation, such as by transgenic techniques. The nucleic acid may be introduced into a cell, directly or indirectly, by introduction into a precursor of the cell by way of deliberate genetic manipulation, such as by microinjection or by infection with a recombinant virus. Genetic manipulation also includes classical cross-breeding, or in vitro fertilization. A recombinant DNA molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA.

[0059] The terms "chimeric animal" or "mosaic animal" are used herein to refer to animals in which the recombinant gene is found, or in which the recombinant is expressed, in some but not all cells of the animal. The term "tissue-specific chimeric animal" indicates that the recombinant gene is present and/or expressed in some tissues but not others. As used herein, the term "transgene" means a nucleic acid sequence that is partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the animal's genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout).

[0060] The term "chromosomal positional effect" as used herein refers to the variation in the degree of gene transcription as a function of the location of the transcribed locus within the cell genome. Random transgenesis may result in a transgene being inserted at different locations in the genome so that individual cells of a population of transgenic cells may each have at least one transgene, each at a different location and therefore each in a different genetic environment. Each cell, therefore, may express the transgene at a level specific for that particular cell and dependent upon the immediate genetic environment of the transgene.

[0061] In a transgenic animal, as a consequence, different tissues may exhibit different levels of transgene expression. The term "reduced chromosomal positioning effect" as used herein refers to a decreased intercellular variation in the level of gene transcription because of a reduction in the number of sites of insertion of a heterologous nucleic acid molecule into the genome of a recipient cell. Consequently, a reduced chromosomal positioning effect provides a more uniform population of genetically transformed cells with respect to transgene insertion sites in the cellular genomes. In transgenic animals, different tissues may exhibit reduced variability in the levels of transgene expression.

[0062] The term "cytokine" as used herein refers to any secreted polypeptide that affects a function of cells and modulates an interaction between cells in the immune, inflammatory or hematopoietic response. A cytokine includes, but is not limited to, monokines and lymphokines. Examples of cytokines include, but are not limited to, interferon .alpha.2b, Interleukin-1 (IL-1), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Tumor Necrosis Factor-.alpha. (TNF-.alpha.) and Tumor Necrosis Factor-.beta. (TNF-.beta.).

[0063] The term "antibody" as used herein refers to polyclonal and monoclonal antibodies and fragments thereof, and immunologic binding equivalents thereof. Antibodies may include, but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single chain antibodies, Fab fragments, F(ab').sub.2 fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, and epitope-binding fragments of any of the above.

[0064] The term "immunoglobulin polypeptide" as used herein refers to a constituent polypeptide of an antibody or a polypeptide derived therefrom. An "immunological polypeptide" may be, but is not limited to, an immunological heavy or light chain and may include a variable region, a diversity region, joining region and a constant region or any combination, variant or truncated form thereof. The term "immunological polypeptides" further includes single-chain antibodies comprised of, but not limited to, an immunoglobulin heavy chain variable region, an immunoglobulin light chain variable region and optionally a peptide linker.

[0065] Techniques useful for isolating and characterizing the nucleic acids and proteins of the present invention are well known to those of skill in the art and standard molecular biology and biochemical manuals may be consulted to select suitable protocols without undue experimentation. See, for example, Sambrook et al., 1989, "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor, the content of which is herein incorporated by reference in its entirety.

Abbreviations

[0066] Abbreviations used in the present specification include the following: aa, amino acid(s); bp, base pair(s); kb, kilobase; cDNA, DNA complementary to RNA; SSC, sodium chloride-sodium citrate; DMSO, dimethyl sulfoxide; MAR, matrix attachment region; CPE, chromosomal positioning effect; BAC, bacterial artificial chromosome; YAC, yeast artificial chromosome.

[0067] The present invention provides novel isolated and recombinant nucleic acid molecules comprising an avian ovalbumin transcriptional regulatory region and at least one MAR element, which are useful as vectors for inserting a heterologous nucleic acid molecule into the genome of a recipient avian cell. The novel isolated nucleic acid molecules of the present invention are particularly useful for directing the incorporation of a heterologous nucleic acid that is under transcriptional regulation of an avian ovalbumin gene promoter, into the genome of a recipient avian cell while reducing or avoiding chromosomal positioning effects that would otherwise result from randomly distributed insertions of the heterologous nucleic acid molecule into the recipient avian genome. The present invention further provides methods of delivering a heterologous nucleic acid under the transcriptional regulation of an avian ovalbumin transcriptional regulatory region, to an avian cell, whereby the heterologous nucleic acid desired to be expressed under the associated avian ovalbumin gene transcriptional regulatory element can be integrated into an avian cell genome. As well as providing recombinant nucleic acids, vectors and derivatives thereof, the present invention provides transfected and transgenic avian cells and birds derived therefrom that are capable of producing a heterologous polypeptide in the serum or the white of a laid egg.

Nucleic Acids Comprising the Chicken Ovalbumin Zene and 5' and 3' MAR Elements

[0068] The novel isolated and recombinant nucleic acid molecules of the present invention comprise the chicken ovalbumin gene comprising transcriptional regulatory elements positioned 5' upstream of the ovalbumin-encoding region of the native chicken ovalbumin locus and which are necessary for the regulated expression of a downstream polypeptide-encoding nucleic acid, and at least one MAR element.

[0069] The inclusion of a MAR element, and preferably at least two MARs, in the same nucleic acid and flanking the ovalbumin gene region, may confer positional independence to a transfected gene operably linked to the ovalbumin transcriptional regulatory region. While not wishing to be bound by any one theory, it is believed that the 5' and 3' MARs of a transfected nucleic acid molecule of the present invention restrict the number of possible transgene insertion sites within the genome of the recipient avian cell, thereby reducing chromosomal positioning effects upon transcription levels. Thus the isolated novel nucleic acid molecules of the present invention are useful for reducing the chromosomal positional effects exerted on heterologous transgene expression. The heterologous transgene will be operably linked to the ovalbumin transcriptional regulatory region within a novel recombinant nucleic acid molecule transfected into a recipient avian cell. Included in the nucleic acid molecules of the present invention are a region of the avian genome encompassing a MAR upstream of the ovalbumin locus and cis-regulatory elements that may allow gene expression in a tissue-specific manner. The ovalbumin promoter region of the novel nucleic acid molecules is especially useful for directing expression of an operably linked heterologous nucleic acid in a transfected avian cell such as an avian oviduct cell.

[0070] Also within the scope of the present invention that nucleic acid molecules further comprising a region of the chicken ovalbumin locus that is 3' of the ovalbumin-encoding region, or of a nucleic acid insert encoding a heterologous polypeptide. The ovalbumin 3' domain included in the nucleic acid molecules of the present invention includes at least one nucleic acid sequence encoding a 3' MAR element which may cooperate with a 5' MAR to limit the number of sites of insertion into the genome of an avian cell of a transfected nucleic acid molecule. In either event, the directed insertion induced by one or more MARs can reduce or eliminate chromosomal positioning effects, resulting in a more uniform level of gene expression of the heterologous nucleic acid insert in a population of genetically transformed cells.

(a) Isolated Nucleic Acid Encompassing the Chicken Ovalbumin Gene

[0071] One aspect of the present invention, therefore, is a nucleic acid molecule isolated from the genome of a chicken and comprising a proximal ovalbumin promoter suitable for directing transcription of regulation of a transcript encoding ovalbumin, and 5' and 3' MAR elements flanking the ovalbumin gene region.

[0072] BACs 120 and 77 containing overlapping regions of the chicken genome, were sequenced and compiled as the contiguous sequence SEQ ID NO: 1. BAC 120 includes the sequence from nucleotide position 1 to position 157354 of SEQ ID NO: 1. The sequence of BAC 77 begins at nucleotide position 157355 of SEQ ID NO: 1 to position 195102. The nucleic sequence of the 195,102 bp chicken genomic region SEQ ID NO: 1 is shown in FIG. 1. A schematic showing identifiable domains within SEQ ID NO: 1 that have sequence identity or homology to known domain families or previously identified genes mainly identified using BLAST, GenScan and MARWIZ software is shown in FIG. 2. BAC 26, constructed as described in Example 1 below and containing the entire nucleic acid insert SEQ ID NO: 1 less about 11.5 kb at the extreme 5' end, was deposited with American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va. 20110, as ATCC No. PTA-5548 on Sep. 24, 2003, under the conditions set forth in the Budapest Treaty.

[0073] The nucleic acid molecule SEQ ID NO: 1 of the present invention has at least four MAR elements. One MAR element is 5' upstream of the ovalbumin gene, between about nucleotide positions 41701 and 41900. MAR-like elements are also between nucleotide positions 56001-56201, 56501-56901, 58401-58701, 76251-76451 and 80151-80451. Another MAR element is between about 96401-96800. MAR elements located 3' downstream of the ovalbumin gene are at nucleotide positions about 144651-144850, about 150601-151600, about 156681-157181, about 157081-15781, about 163701-164100, about 186201-186590 and about 190101-190800 of SEQ ID NO: 1. The chicken ovalbumin gene ATG start codon is at nucleotide position 133372.

[0074] Also dispersed along the nucleic acid molecule represented by SEQ ID NO: 1 are other identifiable domains listed, for example, in Table 2 below, including several serpin- or serpin-like encoding genes, cis transcription regulatory elements of both the serpin-like and ovalbumin genes, and at least two other, putatively functional genes, X and Z. Between the various domains, genes or other elements are stretches of nucleotides, the functions of which may serve to maintain the position and configuration of the elements relative to each other.

[0075] The isolated nucleic acids of the present invention and derivatives and truncated variants thereof may be incorporated into a vector, such as a bacterial or yeast artificial chromosome. The BAC cloning system (Shizuya et al, Proc. Natl. Acad. Sci (U.S.A.), 89:8794:8797, (1992) has been developed to stably maintain large fragments of genomic DNA (100-300 kb) in E. coli. An exemplary BAC vector consists of the pBeloBAC11 vector that has been described by Kim et al, Genomics, 34:213:218 (1996). Genomic DNA can be partially digested, for example, using enzymes that permit ligation into either the BamH I or Hind III sites in the vector. It is contemplated that any suitable restriction sites may be used that are useful for incorporating genomic DNA into a selected BAC vector. Flanking these cloning sites are T7 and SP6 RNA polymerase transcription initiation sites that can be used to generate end probes by either RNA transcription or PCR methods. BAC DNA is purified from the host cell as a supercoiled circle. Converting these circular molecules into a linear form precedes both size determination and introduction of the BACs into recipient avian cells. A suitable cloning site may be flanked, for example, by two Not I restriction sites, permitting cloned segments to be excised from the vector by Not I digestion. Alternatively, the BAC vector may be linearized by treatment with the commercially available enzyme lambda terminase that leads to the cleavage at a cosN site. However, this cleavage method results in a full length BAC clone containing both the insert DNA and the BAC vector sequences.

[0076] One embodiment of the novel isolated nucleic acid molecules of the present invention, therefore, is an isolated chicken nucleic acid molecule encoding an ovalbumin transcriptional regulatory region and a 5' MAR. In one embodiment of the present invention, the novel isolated nucleic acid molecule further comprises a 3' MAR downstream of the ovalbumin gene. The isolated nucleic acid molecules of the present invention may also include nucleic acid elements such as, but not limited to, a transcription enhancer element, a negative regulator element, a hormone responsive element, an avian CR1 repeat element that together may constitute, in whole or in part, the ovalbumin transcriptional regulatory region, a proximal ovalbumin promoter and a signal peptide-encoding region. There are also stretches of nucleic acid between these constituent elements that organize the various elements into an ordered linear array. While the constituent elements of the ovalbumin transcriptional control region are preferably ordered as in sequence SEQ ID NO: 1, it is within the scope of the present invention for the cis-elements of the ovalbumin transcriptional regulatory region to be in any linear arrangement that will allow the formation of a transcript comprising the nucleotide sequence, or its complement, of a nucleic acid insert operably linked to the ovalbumin transcriptional regulatory region.

[0077] The novel isolated nucleic acid molecules of the present invention allow one skilled in the art to, for example, (a) make copies of those nucleic acid molecules by procedures such as, but not limited to, insertion into a cell for replication by the cell, by chemical synthesis or by procedures such as PCR or LCR, (b) obtain nucleic acid molecules which include at least a portion of such nucleic acid molecules, including full-length genes, full-length coding regions, transcriptional regulatory sequences, truncated coding regions and the like, (c) identify and obtain ovalbumin transcriptional regulatory region homologs found in other avian species such as, but not limited to, turkey, duck, goose, quail, pheasant, parrot, finch, ratites including ostrich, emu and cassowary and, (d) to obtain isolated nucleic acids capable of hybridizing to an avian ovalbumin transcriptional regulatory region nucleic acid and of being used as a probe to detect the presence of nucleic acid-related sequences by complementation between the probe and the target nucleic acid.

[0078] Such nucleic acid homologs can be obtained in a variety of ways including using traditional cloning techniques to screen appropriate libraries, amplifying appropriate libraries or DNA using oligonucleotide primers derived from the novel nucleic acid molecules of the present invention in a polymerase chain reaction or other amplification method, and screening public and/or private databases containing genetic sequences using nucleic acid sequences of the present invention to identify targets. Examples of preferred libraries to screen, or from which to amplify nucleic acid molecules, include but are not limited to avian BAC libraries, genomic DNA libraries, and cDNA libraries. Similarly, preferred sequence databases useful for screening to identify sequences in other species homologous to chicken ovalbumin transcriptional regulatory region include, but are not limited to, GenBank and the mammalian Gene Index database of The Institute of Genomics Research (TIGR).

[0079] Nucleotides used to construct the nucleic acids of the present invention can be labeled to provide a signal as a means of detection, using conventional labeling technologies such as radioactive labels, fluorescent compounds, enzymes and chemiluminescent moieties. Methods useful in selecting appropriate labels and binding protocols for binding the labels to the synthetic nucleotides are well known to those of skill in the art.

[0080] In one embodiment of the isolated nucleic acid molecule according to the present invention, the nucleic acid is isolated from a chicken.

[0081] In other embodiments of the isolated nucleic acid molecule according to the present invention, the nucleic acid molecule comprises a nucleotide sequence having at least 80% identity, at least 95% identity or at least 99% identity to the nucleotide sequence according to SEQ ID NO: 1, or the complement thereof.

[0082] In other embodiments, the isolated nucleic acid molecule of the invention comprises the nucleotide sequence according to SEQ ID NO: 1 or has the nucleotide sequence according to SEQ ID NO: 1. In another embodiment, the isolated nucleic acid molecule can be an allelic variant of SEQ ID NO: 1.

(b) Fragments and Variants of SEQ ID NO: 1

[0083] Fragments of the isolated nucleic acid molecules of the present invention also are within the scope of the present invention. As used herein, a fragment of a nucleic acid molecule refers to a nucleotide sequence having fewer nucleotides than the nucleotide sequence SEQ ID NO: 1 but which includes a nucleic acid sequence of the ovalbumin transcriptional regulatory region able to direct and regulate transcription of a nucleic acid, and at least one MAR element.

[0084] The isolated nucleic acid molecule having the sequence SEQ ID NO: 1 may be reduced in size by truncating regions that do not affect the expression of a heterologous nucleic acid placed under the transcriptional control of the ovalbumin transcription regulatory region. A truncated variant of the nucleic acid molecule of the present invention is understood to be any variant of SEQ ID NO: 1 less nucleotides at either the 5' and/or the 3' end of SEQ ID NO: 1. For example, it is contemplated that any of the nucleotides from positions 1-about 40500 may be individually, in part, or in total, deleted from the variant nucleic acid molecule. Similarly, nucleotides from positions about 151700, 164200, 186690 or 190900 to 195101 of the nucleic acid molecule having sequence SEQ ID NO: 1 may be removed, retaining 1, 2, 3, 4 or more of the MAR or MAR-like elements respectively located 3' at the chicken ovalbumin gene. Useful truncated variants of SEQ ID NO: 1, therefore, include, but are not limited to, from base position about 41000 to about 191500, to about 187000, to about 164500, to about 152000, or to about 145500 and from base position about 96000 to about 191500, to about 187000, to about 164500, to about 152000 or to about 145500. Other useful truncated variants of SEQ ID NO: 1 include regions from nucleotide positions about 56000, about 58350, about 76200 and about 80000 to about 191500, to about 187000, to about 164500, to about 152000 or to about 145500.

[0085] Therefore, the invention encompasses nucleic acid molecules which do not include regions that do not contribute to the desired functionality of inserting a heterologous nucleic acid into an avian genome with reduced or no chromosomal positioning effect. The region 5' upstream of the MAR located at nucleotide positions 41701-41900 of SEQ ID NO: 1, may be deleted to give a truncated variant of SEQ ID NO: 1. For example, the approximately 11.5 kb region extending from nucleotide position 1 of SEQ ID NO: 1 not present in BAC 26 may be deleted. Likewise, it is contemplated that other regions of SEQ ID NO: 1 as listed in Table 2, such as encoding the serpin-like proteins, may be selectively deleted.

Recombinant Nucleic Acids

[0086] Another aspect of the present invention is recombinant nucleic acid molecules comprising at least one, and preferably at least two, avian MARs and an avian ovalbumin transcription regulatory region, including the proximal promoter thereof. The recombinant nucleic acid molecules of the present invention are particularly useful for delivering a desired heterologous nucleic acid to a recipient avian cell while reducing chromosomal positional effects upon transcription from the integrated heterologous nucleic acid. It is contemplated that regions of SEQ ID NO: 1 may be omitted from the recombinant nucleic acid molecules of the present invention without substantially affecting the reduction in the CPE compared to a similar nucleic acid molecule not including MAR elements. For example, one or more of the serpin-encoding regions of SEQ ID NO: 1 listed in Table 2, below, may not be included.

[0087] The present invention, therefore, provides recombinant nucleic acid molecules that comprise at least one avian MAR and an avian ovalbumin transcription regulatory region optionally operably linked in a linear array to a selected heterologous or endogenous polypeptide-encoding nucleic acid insert, and which may express the nucleic acid insert when transfected to a suitable host cell, preferably an avian cell.

[0088] The nucleic acid insert, such as a heterologous nucleic acid can be operably linked 3' downstream of the ovalbumin proximal promoter and is thereby expressed as an RNA transcript by a transfected recipient cell. The heterologous nucleic acid may be inserted into the recombinant nucleic acid of the present invention 3' downstream of a region encoding a peptide leader region so that a heterologous polypeptide encoded by the inserted nucleic acid may include this leader region. It is within the scope of the present invention for the recombinant nucleic acid to have the nucleic acid insert encoding the desired polypeptide to be operably inserted into the ovalbumin coding region, or operably replacing the ovalbumin coding region in whole or in part. The generation of BACs comprising a heterologous nucleic acid under the transcriptional control of the ovalbumin gene control region according to the present invention are described in Examples 2 and 3, below.

[0089] To increase the efficiency of expression of the heterologous nucleic acid insert, a polyadenylation signal region may be included at the 3' end of the inserted nucleic acid to allow the transcript directed by the novel ovalbumin transcriptional regulatory region to proceed beyond the nucleic acid insert encoding a selected polypeptide thereby providing a transcript further comprising a 3' untranslated region and a polyadenylated tail. Any suitable functional polyadenylation signal sequence may be linked to the 3' end of the nucleic acid insert including, for example, the SV40 polyadenylation signal sequence SEQ ID NO: 2 as shown in FIG. 4, bovine growth hormone adenylation sequence or the like. It is further anticipated that the recombinant nucleic acid molecules of the present invention may comprise the chicken ovalbumin 3' domain, or a variant thereof. The ovalbumin 3' domain may comprise the ovalbumin 3' untranslated region, an ovalbumin gene polyadenylation sequence and at least one of the 3' MAR elements identified downstream of the ovalbumin-encoding region of SEQ ID NO: 1. If the heterologous nucleic acid is inserted within the ovalbumin encoding region, and in-phase with the ovalbumin gene, the polyadenylation signal region of the ovalbumin gene may be used.

[0090] In one embodiment of the recombinant nucleic acid molecule according to the present invention, the recombinant nucleic acid molecule comprises the nucleotide sequence according to SEQ ID NO: 1, or the complement thereof.

[0091] Another aspect of the present invention is a recombinant DNA molecule comprising a MAR element and an avian ovalbumin transcriptional regulatory region. In one embodiment, the ovalbumin transcriptional regulatory region is operably linked in linear array to a nucleic acid insert encoding a polypeptide sought to be expressed, and a polyadenylation signal sequence optionally operably linked thereto. It is contemplated that when the recombinant nucleic acid molecule is to be delivered to a recipient avian cell for expression therein, the sequence of the inserted heterologous nucleic acid sequence may be modified so that the codons thereof are optimized for the codon usage of the recipient species as described below.

[0092] In a preferred embodiment, a MAR element is located 5' upstream of the ovalbumin transcriptional regulatory region. Suitable MAR elements, for example, are located at about nucleotide positions about 41701-41800 and about 96401-96800 of sequence SEQ ID NO: 1.

[0093] In one embodiment of the present invention, the recombinant nucleic acid molecule comprises the nucleotide sequence from nucleotides position about 40750 to 195101 of SEQ ID NO: 1. Various embodiments of the recombinant nucleic acid molecules of the present invention comprise a 5' MAR and/or a 3' MAR, and the ovalbumin transcriptional regulatory region. In one embodiment, the recombinant nucleic acid further comprises a T gene. In others, the recombinant nucleic acid further comprises at least one nucleic acid region selected from the group consisting of the U serpin gene, a V serpin gene, an X gene, a Y gene and a Z serpin derived from SEQ ID NO: 1.

[0094] Another embodiment of the recombinant nucleic acid molecules further comprises at least one avian MAR 3' downstream of the nucleic acid insert. Suitable MAR elements for inclusion 3' downstream of the ovalbumin transcriptional regulatory region of the recombinant construct of the present invention are found at nucleotide positions about 144651-144850, about 150800-151600, about 163701-164100, about 186201-186590 and about 190101-190800 of sequence SEQ ID NO: 1. In one embodiment of the recombinant nucleic acid molecules, the ovalbumin transcriptional regulatory region, the avian 5' MAR, and the avian 3' MAR are independently capable of hybridizing under high stringency conditions to the nucleic acid sequence according to SEQ ID NO: 1, or the complement thereof.

[0095] In various embodiments of the present invention, the recombinant nucleic acid molecule is inserted into a vector such as, but not limited to, a plasmid or viral vector.

[0096] Other embodiments of the recombinant nucleic acid molecules further comprise a plasmid or viral origin of replication. In one embodiment, the recombinant nucleic acid molecule is a bacterial or yeast artificial chromosome.

[0097] Yet another embodiment of the recombinant nucleic acid molecule according to the present invention, therefore, is a recombinant nucleic acid molecule comprising an avian ovalbumin transcription regulatory region, an avian 5' MAR, a heterologous nucleic acid encoding a heterologous polypeptide desired to be expressed by a recipient genetically modified cell, a polyadenylation signal sequence, and an avian 3' MAR, wherein the avian ovalbumin transcription regulatory region, 5' MAR, and the 3' MAR each independently hybridizes under high stringency conditions to the nucleic acid sequence SEQ ID NO: 1, or a complement thereof.

[0098] Polypeptide expression under the control of an avian ovalbumin promoter

[0099] Another aspect of the present invention of the novel isolated ovalbumin transcriptional regulatory region is increasing the amount of a heterologous protein present in a bird (especially the chicken) by gene transfer. Typically, a heterologous polypeptide-encoding nucleic acid insert transferred into the recipient animal host will be operably linked with the ovalbumin transcriptional regulatory region to allow the cell to initiate and continue production of the genetic product protein. A recombinant DNA molecule of the present invention can be transferred into the extra-chromosomal or genomic DNA of the host.

[0100] A useful application of the novel isolated and recombinant nucleic acid molecules of the present invention is to increase the amount of a heterologous protein present in a bird, (especially the chicken) by gene transfer. Typically, a heterologous polypeptide-encoding nucleic acid insert transferred into the recipient bird host or an isolated cell or cell-line from the bird will be operably linked with the ovalbumin transcriptional regulatory region to allow the cell to initiate and continue production of the genetic protein product.

[0101] The isolated nucleic acid molecule SEQ ID NO: 1 is useful for inserting therein a heterologous nucleic acid that is desired to be expressed as a transcript or, ultimately, as a polypeptide. A heterologous nucleic acid may be operably linked to the proximal promoter region of the ovalbumin gene at any position 3' downstream of the promoter that allows transcription from the heterologous nucleic acid and synthesis of the desired encoded peptide.

[0102] Some, or all, of the ovalbumin-encoding region of the isolated or recombinant nucleic acids of the present invention may be replaced by a heterologous nucleic acid to be expressed under the transcriptional control of upstream ovalbumin gene control region. The heterologous nucleic acid may be inserted into the isolated or recombinant nucleic acids of the present invention so that the expressed amino acid sequences derived from the ovalbumin may be linked to the expressed heterologous protein either at the N-terminus or C-terminus thereof.

[0103] Any of the vectors of the present invention may also optionally include a sequence encoding a signal peptide that directs secretion of the protein expressed by the vector from the transgenic cells, for instance, from tubular gland cells of the oviduct. This aspect of the invention effectively broadens the spectrum of exogenous proteins that may be deposited in avian eggs using the methods of the invention. Where an exogenous protein would not otherwise be secreted, the vector bearing the coding sequence is modified to comprise, for instance, about 60 bp encoding a signal peptide. The DNA sequence encoding the signal peptide is inserted in the vector such that the signal peptide is located at the N-terminus of the protein encoded by the vector.

[0104] The expression vectors of the present invention comprise avian ovalbumin transcriptional regulatory regions that can direct expression of either fusion or non-fusion proteins. With fusion vectors, a number of amino acids are usually added to the desired expressed target gene sequence such as, but not limited to, a protein sequence for thioredoxin. A proteolytic cleavage site may further be introduced at a site between the target recombinant protein and the fusion sequence. Additionally, a region of amino acids such as a polymeric histidine region may be introduced to allow binding of the fusion protein to metallic ions such as nickel bonded to a solid support, for purification of the fusion protein. Once the fusion protein has been purified, the cleavage site allows the target recombinant protein to be separated from the fusion sequence. Enzymes suitable for use in cleaving the proteolytic cleavage site include, but are not limited to, Factor Xa and thrombin. Fusion expression vectors that may be useful in the present invention include pGex (Amrad Corp., Melbourne, Australia), pRIT5 (Pharmacia, Piscataway, N.J.) and pMAL (New England Biolabs, Beverly, Mass.), that fuse glutathione S-transferase, protein A, or maltose E binding protein, respectively, to the target recombinant protein.

[0105] The present invention further relates to nucleic acid vectors and transgenes derived therefrom that incorporate polypeptide-encoding regions, wherein a first polypeptide-encoding region is operatively linked to an avian ovalbumin promoter and a second polypeptide-encoding region is operatively linked to an Internal Ribosome Entry Sequence (IRES). It is contemplated that the first polypeptide-encoding region, the IRES and the second polypeptide-encoding region of a recombinant DNA of the present invention may be arranged linearly, with the IRES operably positioned immediately 5' of the second polypeptide-encoding region. This nucleic acid construct, when inserted into the genome of a bird and expressed therein, will generate individual polypeptides that may be post-translationally modified and combined in the white of a hard-shell bird egg. Alternatively, the expressed polypeptides may be isolated from an avian egg and combined in vitro.

[0106] Expression of a heterologous nucleic acid by a recombinant expression vector according to the present invention can be obtained using eukaryotic host cells, preferably avian cells, more preferably chicken cells, and still more preferably chicken oviduct cells, especially tubular gland cells. The use of eukaryotic host cells permit partial or complete post-translational modification such as, but not only, glycosylation and/or the formation of the relevant inter- or intra-chain disulfide bonds. Examples of vectors useful for expression in the chicken Gallus gallus include pYepSec1 as in Baldari et al., E.M.B.O.J., 6, 229-234 (1987) and pYES2 (Invitrogen Corp., San Diego, Calif.), incorporated herein by reference in their entireties.

[0107] One aspect of the present invention is methods of delivering a novel nucleic acid molecule of the present invention to the cytoplasm of an avian cell having a nucleus, thereby generating a transfected and genetically transformed avian cell. Such incorporation can be carried out by the various forms of transfection, depending upon the vector/host cell system. It is contemplated that the incorporation of recombinant nucleic acid molecules of the present invention into a recipient cell may be by any suitable method such as, but not limited to, viral transfer, electroporation, gene gun insertion, sperm-mediated transfer to an ovum, microinjection and the like.

[0108] In the various embodiments of these methods, the avian cell may be a chicken cell or a quail cell. In some embodiments of the methods of the present invention, the avian cell is within oviductal tissue of a bird, an isolated oviduct cell or primary cell line, or a sustainable oviduct cell line. Preferably, the oviduct cells are tubular gland cells.

[0109] Heterologous polypeptide can be produced by transfected cells of the invention in vitro, i.e., in tissue culture outside the body of a living animal. Alternatively, the nucleic acids of the present invention may be delivered to an animal such as a chicken, whereupon the nucleic acid may enter cells and be expressed therein. It is anticipated that the nucleic acids of the present invention may integrate into the genome of the recipient cells and then express the encoded, typically heterologous, polypeptide therein. Preferably, a heterologous nucleic acid is delivered to oviduct cells within a chicken for synthesis of the desired polypeptide and its deposition in the white of an egg.

[0110] Another aspect of the present invention is a eukaryotic cell transfected with an expression vector according to the present invention and described above. For example, in one embodiment, the transformed cell can be a chicken oviduct cell or cell line, including a sustainable cell line, and the transfected nucleic acid insert comprises the chicken ovalbumin transcriptional regulatory region, a 5' MAR and/or a 3' MAR, a nucleic acid insert encoding a human interferon .alpha.2b and codon optimized for expression in an avian cell, and an SV40 polyadenylation sequence. In another example, the nucleic acid insert encodes an immunoglobulin heavy chain and a second chain under the transcriptional control of an IRES.

[0111] The transfected cell according to the present invention may be transiently transfected, whereby the transfected recombinant nucleic acid, such as DNA, or expression vector may not be integrated into the genomic nucleic acid. However, the transfected recombinant DNA or expression vector may be stably integrated into the genomic DNA of the recipient cell, thereby replicating with the cell so that each daughter cell receives a copy of the transfected nucleic acid. When the recombinant DNA or expression vector of the present invention is integrated into the genomic DNA of the recipient cell so that the cell is genetically transformed, it is anticipated that the MAR element(s) of the integrated nucleic acid will direct integration a limited number of integration site within the target genome, thereby producing a population of cells more uniform with regard to the level of expression of the heterologous nucleic acid.

[0112] The present invention also includes a transgenic bird producing a heterologous protein expressed from a transfected nucleic acid according to the present invention. The transgenic bird is selected from a turkey, duck, goose, quail, pheasant, ratite, an ornamental bird or a feral bird. In a preferred embodiment, the avian is a chicken and the heterologous protein produced under the transcriptional control of the avian ovalbumin transcriptional regulatory region according to the present invention is produced in the white of an egg.

Viral Host Cell Transformation

[0113] Nucleic acid sequences or derivative or truncated variants thereof, may be introduced into viruses such as an adenovirus or vaccinia virus. Methods for making a viral recombinant vector useful for expressing a protein under the control of the ovalbumin promoter are analogous to the methods disclosed in U.S. Pat. Nos. 4,603,112; 4,769,330; 5,174,993; 5,505,941; 5,338,683; 5,494,807; 4,722,848; Paoletti, E., 1996, Proc. Natl. Acad. Sci. 93: 11349-11353; Moss, 1996, Proc. Natl. Acad. Sci. 93: 11341-11348; Roizman 1996, Proc. Natl. Acad. Sci. 93: 11307-11302; Frolov et al., 1996, Proc. Natl. Acad. Sci. 93: 11371-11377; Grunhaus et al., 1993, Seminars in Virology 3: 237-252 and U.S. Pat. Nos. 5,591,639; 5,589,466; and 5,580,859 relating to DNA expression vectors, inter alia, the contents of which are incorporated herein by reference in their entireties.

[0114] Retrovirus vectors and adeno-associated virus vectors provide efficient systems of delivery of genes into cells, and the transferred nucleic acids may be stably integrated into the chromosomal DNA of the host. Protocols for producing recombinant retroviruses and for infecting cells in vitro or in vivo with such viruses can be found in Ausubel et al., 1989, Current Protocols in Molecular Biology .sctn..sctn. 9.10-9.14 and other standard laboratory manuals. Examples of suitable retroviruses include pLJ, pZIP, pWE and pEM which are well known to those skilled in the art. Examples of suitable packaging virus lines for preparing both ecotropic and amphotropic retroviral systems include psiCrip, psiCre, psi2 and psiAm.

[0115] Furthermore, it is possible to limit the infection spectrum of retroviruses and consequently of retroviral-based vectors, by modifying the viral packaging proteins on the surface of the viral particle (see, for example PCT publications WO 93/25234, WO 94/06920, and WO 94/11524). Roux et al., 1898, Proc. Natl. Acad. Sci. 86:9079-9083; Julan et al., 1992, J. Gen. Virol. 73:3251-3255; and Goud et al., 1983, Virology 163:251-254); Neda et al., 1991, J. Biol. Chem. 266:14143-14146), which are incorporated herein by reference in their entireties.

[0116] One retrovirus for randomly introducing a transgene into the avian genome is a replication-deficient ALV retrovirus. To produce an appropriate ALV retroviral vector, a pNLB vector may be modified by inserting a region comprising at least part of the ovalbumin transcriptional regulatory region, a MAR element and one or more exogenous genes between the 5' and 3' long terminal repeats (LTRs) of the retrovirus genome. Any coding sequence placed in-frame and downstream of the ovalbumin promoter will be expressed at high levels and especially in the tubular gland cells of the oviduct magnum because the ovalbumin promoter drives the high level of expression of the ovalbumin protein and is only active in the oviduct tubular gland cells.

[0117] Another viral gene delivery system useful in the present invention utilizes adenovirus-derived vectors (see, for example, Berkner et al., 1988, BioTechniques 6:616-629; Rosenfeld et al., 1991, Science 252:431-434; and Rosenfeld et al., 1992, Cell 68:143-155), incorporated herein by reference in their entireties. Suitable adenoviral vectors derived from the adenovirus strain Ad type 5 dl324 or other strains of adenovirus (e.g., Ad2, Ad3, Ad7 etc.) are well known to those skilled in the art. Introduced adenoviral DNA (and foreign DNA contained therein) is not integrated into the genome of a host cell but remains episomal, thereby avoiding potential problems that can occur as a result of insertional mutagenesis in situations where introduced DNA becomes integrated into the host genome (e.g., retroviral DNA).

[0118] Yet another viral vector system is the adeno-associated virus (AAV). Vectors containing as little as 300 base pairs of AAV can be packaged and can integrate. In the present invention, at least part of the heterologous nucleic acid will include an operable region of the avian ovalbumin transcriptional regulatory region and a MAR element. An AAV vector such as that described in Tratschin et al., 1985, Mol. Cell. Biol. 5:3251-3260, can be used to introduce DNA into cells.

[0119] Other viral vector systems that may have application in the methods according to the present invention have been derived from, but are not limited to, herpes viruses, vaccinia viruses, avian leucosis viruses and several RNA viruses.

Non-Viral Expression Vectors

[0120] Most non-viral methods of gene transfer rely on normal mechanisms used by eukaryotic cells for the uptake and intracellular transport of macromolecules. In preferred embodiments, non-viral gene delivery systems of the present invention rely on endocytic pathways for the uptake of the subject ovalbumin transcriptional regulatory region and operably linked polypeptide-encoding nucleic acid by the targeted cell. Exemplary gene delivery systems of this type include liposomal derived systems, poly-lysine conjugates, and artificial viral envelopes.

[0121] In a representative embodiment, a nucleic acid comprising the novel recombinant nucleic acids of the present invention can be entrapped in liposomes bearing positive charges on their surface (e.g., lipofectins) and (optionally) which are tagged with antibodies against cell surface antigens of the target tissue (Mizuno et al., 1992, NO Shinkei Geka 20: 547-551; PCT publication WO91/06309; Japanese patent application 1047381; and European patent publication EP-A-43075, all of which are incorporated herein by reference in their entireties).

[0122] In similar fashion, the gene delivery system comprises an antibody or cell surface ligand that is cross-linked with a gene binding agent such as polylysine (see, for example, PCT publications WO93/04701, WO92/22635, WO92/20316, WO92/19749, and WO92/06180, all of which are incorporated herein by reference in their entireties). It will also be appreciated that effective delivery of the subject nucleic acid constructs via receptor-mediated endocytosis can be improved using agents which enhance escape of genes from the endosomal structures. For instance, whole adenovirus or fusogenic peptides of the influenza HA gene product can be used as part of the delivery system to induce efficient disruption of DNA-containing endosomes (Mulligan et al., 1993, Science 260-926; Wagner et al., 1992, Proc. Natl. Acad. Sci. 89:7934-7938; and Christiano et al., 1993, Proc. Natl. Acad. Sci. 90:2122-2126, all of which are incorporated herein by reference in their entireties). It is further contemplated that a recombinant DNA molecule of the present invention may be delivered to a recipient host cell by other non-viral methods including by gene gun, microinjection, sperm-mediated transfer, or the like.

[0123] Another aspect of the present invention is a method of expressing a heterologous polypeptide in a eukaryotic cell by transfecting a cell with a recombinant nucleic acid molecule of the invention, as described above, and culturing the transfected cell under conditions suitable for expression of the heterologous polypeptide under the control of the avian ovalbumin transcriptional regulatory region.

[0124] In one embodiment of this aspect, the nucleic acid molecule is integrated into the genome of the recipient avian cell. In some embodiments the recipient avian oviduct cell is a chicken cell, preferably a chicken oviduct cell, more preferably an oviduct tubular gland cell.

[0125] The protein of the present invention may be produced in purified form by any known conventional techniques. For example, chicken cells, an egg or an egg white may be homogenized and centrifuged. The supernatant may then be subjected to sequential ammonium sulfate precipitation and heat treatment. The fraction containing the protein of the present invention is subjected to gel filtration in an appropriately sized dextran or polyacrylamide column to separate the proteins. If necessary, the protein fraction may be further purified by HPLC or other methods well known in the art of protein purification.

Expression of Heterologous Multimeric Proteins by Transfected Avian Cells

[0126] The present invention provides methods for the production of a multimeric protein by an avian cell, comprising the step of culturing an avian cell transfected with a first expression vector and, optionally, a second expression vector; the expression vectors may each have a transcription unit comprising a nucleotide sequence encoding a first heterologous polypeptide, a transcription promoter, and a transcriptional terminator operatively linked to the nucleotide sequence encoding a second heterologous polypeptide, such that the cultured avian cell produces a multimeric protein comprising the first and second heterologous polypeptides.

[0127] The isolated nucleic acids and recombinant nucleic acid constructs derived therefrom of the present invention are useful to express nucleic acid sequences of polypeptides that are optimized for expression in avian cells, and derivatives and fragments thereof. Such derivatives include, for instance, polypeptides with conservative amino acid replacements, that is, those within a family of amino acids that are related in their side chains (commonly known as acidic, basic, nonpolar, and uncharged polar amino acids). Phenylalanine, tryptophan, and tyrosine are sometimes classified jointly as aromatic amino acids and other groupings are known in the art (see, for example, "Biochemistry", 2nd ed, L. Stryer, ed., WH Freeman and Co., 1981). Peptides in which more than one replacement has taken place can readily be tested for activity in the same manner as derivatives with a single replacement, using conventional polypeptide activity assays (e.g. for enzymatic or ligand binding activities).

[0128] Regarding codon optimization, for example, if the recombinant DNA is transfected into a recipient chicken cell, the sequence of the nucleic acid insert to be expressed is optimized for chicken codon usage. This may be determined from the codon usage of at least one, and preferably more than one, protein expressed in a chicken cell according to well known principles. For example, in the chicken the codon usage may be determined from the nucleic acid sequences encoding the proteins lysozyme, ovalbumin, ovomucin and ovotransferrin of chicken. Optimization of the sequence for codon usage elevates the level of translation in avian eggs.

[0129] One embodiment of the recombinant nucleic acid of the present invention, comprises an insert encodes the human interferon .alpha.2b polypeptide. The exemplary nucleic acid sequence SEQ ID NO: 3 (FIG. 5) encodes the polypeptide human interferon .alpha.2b in accordance with avian cell codon usage, as determined from the nucleotide sequences encoding chicken ovomucin, ovalbumin, ovotransferrin and lysozyme.

[0130] The invention methods for producing multimeric proteins include immunoglobulins, such as antibodies, and antigen binding fragments thereof. Thus, in one embodiment of the present invention, the multimeric protein is an immunoglobulin, wherein the first and second heterologous polypeptides are an immunoglobulin heavy and light chains respectively. Illustrative examples of this and other aspects of the present invention for the production of heterologous multimeric polypeptides in avian cells are fully disclosed in U.S. patent application Ser. No. 09/877,374, filed Jun. 8, 2001, by Rapp, published as US-2002-0108132-A1 on Aug. 8, 2002, and U.S. patent application Ser. No. 10/251,364, filed Sep. 18, 2002, by Rapp, now U.S. Pat. No. 7,312,374 issued Dec. 25, 2007, both of which are incorporated herein by reference in their entirety.

[0131] Accordingly, the invention further provides immunoglobulin and other multimeric proteins that have been produced by transgenic avians of the invention.

[0132] In various embodiments, an immunoglobulin polypeptide encoded by the transcriptional unit of at least one expression vector may be an immunoglobulin heavy chain polypeptide comprising a variable region or a variant thereof, and may further comprise a D region, a J region, a C region, or a combination thereof. An immunoglobulin polypeptide encoded by an expression vector may also be an immunoglobulin light chain polypeptide comprising a variable region or a variant thereof, and may further comprise a J region and a C region. The present invention also contemplates multiple immunoglobulin regions that are derived from the same animal species, or a mixture of species including, but not only, human, mouse, rat, rabbit and chicken. In preferred embodiments, the antibodies are human or humanized.

[0133] In other embodiments, the immunoglobulin polypeptide encoded by at least one expression vector comprises an immunoglobulin heavy chain variable region, an immunoglobulin light chain variable region, and a linker peptide thereby forming a single-chain antibody capable of selectively binding an antigen.

[0134] Another aspect of the present invention provides a method for the production in an avian of an heterologous protein capable of forming an antibody suitable for selectively binding an antigen. This method comprises a step of producing a transgenic avian incorporating at least one transgene, wherein the transgene encodes at least one heterologous polypeptide selected from an immunoglobulin heavy chain variable region, an immunoglobulin heavy chain comprising a variable region and a constant region, an immunoglobulin light chain variable region, an immunoglobulin light chain comprising a variable region and a constant region, and a single-chain antibody comprising two peptide-linked immunoglobulin variable regions.

[0135] In one embodiment of this method, the isolated heterologous protein is an antibody capable of selectively binding to an antigen which may be generated by combining at least one immunoglobulin heavy chain variable region and at least one immunoglobulin light chain variable region, preferably cross-linked by at least one disulfide bridge. The combination of the two variable regions generates a binding site that binds an antigen using methods for antibody reconstitution that are well known in the art.

[0136] The present invention also encompasses immunoglobulin heavy and light chains, or variants or derivatives thereof, to be expressed in separate transgenic avians, and thereafter isolated from separate media including serum or eggs, each isolate comprising one or more distinct species of immunoglobulin polypeptide. The method may further comprise the step of combining a plurality of isolated heterologous immunoglobulin polypeptides, thereby producing an antibody capable of selectively binding to an antigen. In this embodiment, for instance, two or more individual transgenic avians may be generated wherein one transgenic produces serum or eggs having an immunoglobulin heavy chain variable region, or a polypeptide comprising such, expressed therein. A second transgenic animal, having a second transgene, produces serum or eggs having an immunoglobulin light chain variable region, or a polypeptide comprising such, expressed therein. The polypeptides from two or more transgenic animals may be isolated from their respective sera and eggs and combined in vitro to generate a binding site capable of binding an antigen.

[0137] Examples of therapeutic antibodies that can be used in methods of the invention include but are not limited to HERCEPTIN.RTM. (Trastuzumab) (Genentech, CA) which is a humanized anti-HER2 monoclonal antibody for the treatment of patients with metastatic breast cancer; REOPRO.RTM. (abciximab) (Centocor) which is an anti-glycoprotein IIb/IIIa receptor on the platelets for the prevention of clot formation; ZENAPAX.RTM. (daclizumab) (Roche Pharmaceuticals, Switzerland) which is an immunosuppressive, humanized anti-CD25 monoclonal antibody for the prevention of acute renal allograft rejection; PANOREX.TM. which is a murine anti-17-IA cell surface antigen IgG2a antibody (Glaxo Wellcome/Centocor); BEC2 which is a murine anti-idiotype (GD3 epitope) IgG antibody (ImClone System); IMC-C225 which is a chimeric anti-EGFR IgG antibody (ImClone System); VITAXIN.TM. which is a humanized anti-.alpha.V.beta.3 integrin antibody (Applied Molecular Evolution/MedImmune); Campath 1H/LDP-03 which is a humanized anti CD52 IgG1 antibody (Leukosite); Smart M195 which is a humanized anti-CD33 IgG antibody (Protein Design Lab/Kanebo); RITUXAN.TM. which is a chimeric anti-CD20 IgG1 antibody (IDEC Pharm/Genentech, Roche/Zettyaku); LYMPHOCIDE.TM. which is a humanized anti-CD22 IgG antibody (Immunomedics); ICM3 is a humanized anti-ICAM3 antibody (ICOS Pharm); IDEC-114 is a primatied anti-CD80 antibody (IDEC Pharm/Mitsubishi); ZEVALIN.TM. is a radiolabelled murine anti-CD20 antibody (IDEC/Schering AG); IDEC-131 is a humanized anti-CD40L antibody (IDEC/Eisai); IDEC-151 is a primatized anti-CD4 antibody (IDEC); IDEC-152 is a primatized anti-CD23 antibody (IDEC/Seikagaku); SMART anti-CD3 is a humanized anti-CD3 IgG (Protein Design Lab); 5G1.1 is a humanized anti-complement factor 5 (CS) antibody (Alexion Pharm); D2E7 is a humanized anti-TNF-.alpha. antibody (CATIBASF); CDP870 is a humanized anti-TNF-.alpha. Fab fragment (Celltech); IDEC-151 is a primatized anti-CD4 IgG1 antibody (IDEC Pharm/SmithKline Beecham); MDX-CD4 is a human anti-CD4 IgG antibody (Medarex/Eisai/Genmab); CDP571 is a humanized anti-TNF-.alpha. IgG4 antibody (Celitech); LDP-02 is a humanized anti-.alpha.4.beta.7 antibody (LeukoSite/Genentech); OrthoClone OKT4A is a humanized anti-CD4 IgG antibody (Ortho Biotech); ANTOVA.TM. is a humanized anti-CD40L IgG antibody (Biogen); ANTEGREN.TM. is a humanized anti-VLA-4 IgG antibody (Elan); and CAT-152 is a human anti-TGF-.beta..sub.2 antibody (Cambridge Ab Tech).

Production of Exogenous Protein by Transgenic Avians

[0138] Methods for the production of heterologous protein by the avian oviduct and the production of eggs which contain heterologous protein involve providing a suitable vector and introducing the vector into embryonic blastodermal cells so that the vector can integrate into the avian genome. A subsequent step involves deriving a mature transgenic avian from the transgenic blastodermal cells produced in the previous steps. Deriving a mature transgenic avian from the blastodermal cells optionally involves transferring the transgenic blastodermal cells to an embryo and allowing that embryo to develop fully, so that the cells become incorporated into the bird as the embryo is allowed to develop. Another alternative is to transfer the transfected nucleus to an enucleated recipient cell which may then develop into a zygote and ultimately an adult bird. The resulting chick is then grown to maturity.

[0139] In an alternative embodiment, the cells of a blastodermal embryo are transfected or transduced with the vector directly within the embryo. It is contemplated, for example, that the recombinant nucleic acid molecules of the present invention may also be introduced into a blastodermal embryo by direct microinjection of the DNA into a Stage X or earlier embryo that had been removed from the oviduct. The egg is then returned to the bird for shell development and laying. The resulting embryo is allowed to develop and the chick allowed to mature.

[0140] In either case, the transgenic bird so produced from the transgenic blastodermal cells is known as a "founder" Some founders can be chimeric or mosaic birds if, for example, microinjection does not deliver nucleic acid molecules to all of the blastodermal cells of an embryo. Some founders will carry the transgene in the tubular gland cells in the magnum of their oviducts and will express the exogenous protein encoded by the transgene in their oviducts. If the exogenous protein contains the appropriate signal sequences, it will be secreted into the lumen of the oviduct and onto the yolk of an egg.

[0141] Some founders are germ-line founders. A germ-line founder is a founder that carries the transgene in genetic material of its germ-line tissue, and may also carry the transgene in oviduct magnum tubular gland cells that express the exogenous protein. Therefore, in accordance with the invention, the transgenic bird will have tubular gland cells expressing the exogenous protein and the offspring of the transgenic bird will also have oviduct magnum tubular gland cells that express the exogenous protein. (Alternatively, the offspring express a phenotype determined by expression of the exogenous gene in a specific tissue of the avian.)

[0142] The invention can be used to express, in large yields and at low cost, a wide range of desired proteins including those used as human and animal pharmaceuticals, diagnostics, and livestock feed additives. Proteins such as growth hormones, cytokines, structural proteins and enzymes including human growth hormone, interferon, lysozyme, and .beta.-casein are examples of proteins which are desirably expressed in the oviduct and deposited in eggs according to the invention. Other possible proteins to be produced include, but are not limited to, albumin, .alpha.-1 antitrypsin, antithrombin III, collagen, factors VIII, IX, X (and the like), fibrinogen, hyaluronic acid, insulin, lactoferrin, protein C, erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), tissue-type plasminogen activator (tPA), feed additive enzymes, somatotropin, and chymotrypsin. Immunoglobulins and genetically engineered antibodies, including immunotoxins which bind to surface antigens on human tumor cells and destroy them, can also be expressed for use as pharmaceuticals or diagnostics.

[0143] One aspect of the present invention, therefore, concerns transgenic birds, such as chickens, comprising a recombinant nucleic acid molecule of the present invention and which preferably (though optionally) express a heterologous gene in one or more cells in the animal.

[0144] Suitable methods for the generation of transgenic avians having heterologous DNA incorporated therein are described, for example, in WO 99/19472 to Ivarie et al.; WO 00/11151 to Ivarie et al.; and WO 00/56932 to Harvey et al., all of which are incorporated herein by reference in their entirety.

[0145] In various embodiments of the transgenic bird of the present invention, the expression of the transgene may be restricted to specific subsets of cells, tissues or developmental stages utilizing, for example, trans-acting factors acting on the ovalbumin transcriptional regulatory region of the present invention and which control gene expression in the desired pattern. Tissue-specific regulatory sequences and conditional regulatory sequences can be used to control expression of the transgene in certain spatial patterns. Moreover, temporal patterns of expression can be provided by, for example, conditional recombination systems or prokaryotic transcriptional regulatory sequences. The inclusion of a 5' MAR, and optionally a 3' MAR region, in the novel nucleic acid molecules of the present invention will allow the heterologous expression unit to escape all, or in part, the chromosomal positional effect and therefore be expressed at a more uniform level in transgenic tissues that received the transgene by a route other than through germ line cells.

[0146] In various embodiments of the present invention the transgenic avians comprise a recombinant nucleic acid comprising SEQ ID NO: 1, a truncated variant of SEQ ID NO: 1, or the complement thereof.

[0147] In one embodiment of the present invention, the transgenic avian is selected from the group consisting of a chicken, a turkey, a duck, a goose, a quail, a pheasant, a ratite, an ornamental bird or a feral bird. In a preferred embodiment, the avian is a chicken.

[0148] In various embodiments, the transgenic avian produces the heterologous polypeptide in the serum or an egg white, or both.

[0149] The present invention is further illustrated by the following examples, which are provided by way of illustration and should not be construed as limiting. The contents of all references, published patents and patents cited throughout the present application are hereby incorporated by reference in their entireties.

[0150] It will be apparent to those skilled in the art that various modifications, combinations, additions, deletions and variations can be made in the present invention without departing from the scope or spirit of the invention. For instance, features illustrated or described as part of one embodiment can be used in another embodiment to yield a still further embodiment. It is intended that the present invention covers such modifications, combinations, additions, deletions and variations as come within the scope of the appended claims and their equivalents.

Example 1

Construction of a Complete Ovalbumin Locus from Two Overlapping BACs

[0151] A complete ovalbumin locus BAC was created from two overlapping BACs that together contained the complete ovalbumin locus, as shown in FIG. 6. The nucleotide sequences of BAC 120 and BAC 77 are in opposite directions with respect to the vector backbone pECBAC1.

[0152] BAC 120 was digested with Not I and a 145 kb fragment was re-cloned, but in the reversed orientation (flipped), into Not I digested vector backbone pECBAC1. This resulted in a deletion of a region of approximately 11.5 kb from the 5' end of the insert sequence of BAC 120 and which was upstream of the DNase I sensitivity region. The reversed BAC 120 `flip` and BAC 77 clones were digested with Srf I and RARE digested using an oligonucleotide targeted to an EcoRI site within ovalbumin. 5' and 3' fragments were isolated by CHEF gel electrophoresis, and ligated together to yield the complete contiguous ovalbumin genomic locus BAC.

Example 2

Expression of a Heterologous Gene by a Chicken Ovalbumin Locus BAC

[0153] cDNA constructs encoding immunoglobulin light-chain and heavy-chains of a human IgG1 kappa monoclonal antibody were inserted in-frame with the ovalbumin translation start site of separate ovalbumin locus-containing BACs, as shown in FIG. 3. The immunoglobulin chain-encoding cDNAs were first inserted into a plasmid that contained a 2.7 kb EcoRI fragment from the ovalbumin gene and which included the ovalbumin start site. The resulting vector was then digested with restriction endonuclease EcoR1 and cloned into an approximately 195 kb ovalbumin BAC which had been subjected to EcoR1 recA-assisted restriction endonuclease (RARE) digestion as described by Boren et al., 1996, Prot. Sci. 5,: 2479-2484 and incorporated herein by reference in its entirety.

[0154] Transgenic birds were created by cytoplasmic co-microinjection of human light-chain and heavy chain BACs followed by ovum transfer as described in U.S. patent application Ser. No. 10/251,364 incorporated herein by reference in its entirety.

[0155] A hen carrying these constructs was grown to sexual maturity. Eggs were collected and the egg white material was assayed for the expressed human monoclonal antibody using sandwich ELISA as described by Harlow et al., Antibodies: a Laboratory Manual. 1988, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory. Xiii incorporated herein by reference in its entirety. The human monoclonal antibody was captured by a goat anti-human kappa chain specific monoclonal antibody and quantified with an alkaline phosphatase conjugated goat anti-human gamma detection antibody. Hen # AA698 expressed up to 1025 pg of human monoclonal antibody per ml of egg white.

Example 3

Expression of a Heterologous Gene by a Chicken Ovalbumin Locus BAC

[0156] The open reading frame of the firefly luciferase gene was inserted into the ovalbumin translation start site of an ovalbumin locus BAC as shown in FIG. 3. The luciferase gene was inserted into a plasmid that contained a 2.7 kb EcoR1 fragment from the ovalbumin gene and which includes the ovalbumin start site. The resulting vector was then digested with EcoR1 and cloned into an approximately 195 kb ovalbumin BAC which had been subjected to EcoR1 recA-assisted restriction endonuclease (RARE) digestion as described by Boren et al., 1996, Prot. Sci. 5,: 2479-2484 and incorporated herein by reference in its entirety.

[0157] Primary tubular gland cells isolated from the oviduct of laying quail (Sanders and McKnight, Endocrinology 116, 398-405 (1985)), were transfected using the ovalbumin-luciferase construct or with a negative control CMV-IFN construct. Luciferase activity in cell extracts was analyzed two days post transfection (Table 1).

TABLE-US-00001 TABLE 1 DNA RLU CMV-IFN 60 Ovalbumin Luciferase 274

Example 4

Basic Local Alignment Search Tool (BLAST) Analysis of the Complete Ovalbumin Promoter Sequence (SEQ ID NO: 1)

[0158] The complete approximately 195 kb ovalbumin promoter sequence (SEQ ID NO: 1) was submitted to the National Center for Biotechnology Information for BLAST alignments with database sequences. Further analysis was by using the GenScan and MARWIZ software. Percent identities between the ovalbumin gene region sequence (SEQ ID NO: 1) and corresponding known ovalbumin promoter features are listed in Table 2 below.

TABLE-US-00002 TABLE 2 Nucleotide positions of identifiable elements in the region of the chicken genomic within BACs 120, 77 and 26 Nucleotide Positions.sup.a Domain Identity 5963-1 Q.sup.b 9730-9922 CR1 10772-11935 CpG Island 18914-19088 CR1-GG 20106-20921 CR1-GG 39975-24820 R ATPase 41119-41177 CR1-GG 41586-41700 CR1-GG 41701-41800 MAR element 42221-42742 CpG Island 43505-46990 S Gene 50017-51427 T Gene 56001-56201 MAR-like element 56501-56901 MAR-like element 64599-71919 U Serpin Gene 58401-58701 MAR-like element 74883-75634 CR1-GG 75420-75634 CR1b 76251-76451 MAR-like element 80151-80451 MAR-like element 81125-94938 V Serpin Gene 81832-82120 CR1 85473-85922 CR1-GG 88654-88797 CR1-GG 90120-90167 CR1-GG 96401-96800 MAR element 97884-97965 Y: OV-1 element 99080-99107 SDRE element 100602-107839 X Gene 110247-111200 CR1-GG 114779-121099 Y Gene 117849-118132 CR1-GG 131729-139290 Ovalbumin 144651-144850 MAR element 147721-155849- W Gene 150801-151600 MAR element 156581-157181 MAR-like element 157081-157581 MAR-like element 157132-157331 MAR-like element 159095-165114 MENT 163701-164100 MAR element 171633-180432 Z1 183204-190418 Z2 186201-186590 MAR element 190101-190800 MAR element 192078-195101 Z3 .sup.aNucleotide positions of protein encoding regions are from the beginning of the first exon to the end of the poyadenylation signal-exons are shown in FIG. 1 .sup.bProtein coding regions are given in bold

[0159] Although preferred embodiments of the invention have been described using specific terms, devices, and methods, such description is for illustrative purposes only. The words used are words of description rather than of limitation. It is to be understood that changes and variations may be made by those of ordinary skill in the art without departing from the spirit or the scope of the present invention, which is set forth in the following claims. In addition, it should be understood that aspects of the various embodiments may be interchanged both in whole or in part.

Sequence CWU 1

1

31195102DNAGallus gallus 1tgcaagcttg caagttttac tgtctcatat ggttcacata ccattcattt agcgtggcct 60cacaaccaac ttgggaacca ctgccctaag taacaatacc tcacatatcc ataggatctt 120caacaccatt ctgcttaaaa ttcaaatccc tttcattaaa atagaagata tttagataca 180agataaatag agcatatgtt tgtatgtggt catcttcagt tctagaaaca gtttcttctc 240tcttatgcaa ctgaaagtag cttagtttcc acagccccgt gcagagcaat acggttgtac 300tgctctcaaa gaagtaccaa cacaacatgc atcagcagag ttgcacaaga ctactgaaat 360aaaatttgct tagctgctag tgaaaacata agtatccttg cttgtaaaca aaagcataag 420tcatttcaca cacctccatt tgcagggctt cagctaatcc tcgaagagca aacttggtcg 480gagaataggc tgtataacca aaaaggccta attgcccagc ctgagatgat acaaacacaa 540tccttcccat ccgtcgttcc ttcatggtag agatgactgc acgactcggg taaacactac 600ctagataatt gactgccatt aatctctgtg ggaaaaaaaa aagatattaa aatggtattt 660cagtcactga ttcagaattc ttcagatgtt tagttttatt tctccaaaat ccctactgag 720gcttgcttta acatcaaact ccactacatt tctatcagcg agcaaagtaa acaattgttt 780tctactgtat ttgtttcata aacgttccat agcaaagaat ctcaccgtaa gacaaaaaga 840aatgcactgt atatacaagt gttaatggca ttgaggatac taatgatgtg agctattatg 900gctttcacaa agaaaaaagg gcaaattaca aaaaaaactg acttgaacag tgtctaggaa 960gcaatccatt tatacaagaa gtacaaaaac attgaagtga aagtttaaag ggcttcctaa 1020aaaaaagaaa gaggaaaaat tctatgtata tatgcacaag ctctcatctt ggcaggtaag 1080attaattcct gtttcaatct cctgaagacc taaaacacaa ctggtggcct acacaagcaa 1140taaaacagat tatttaccct tactgttaca cttactataa cattatgcct attcagtatc 1200acgtgcattt tcagatggct ctgtgatgaa gtgagaatca aagtaaccag aaagtagaga 1260ggctgtttgg ttttttttaa tgtattttgg tttgggtttt actgttgttc ctttctttcc 1320accaagctgc accaagttac caacagttta cataccgcag agttgaatgc tgacaagatg 1380gattcttctt taacagattc aaaatatggg aaaaagaccc acacatcttc accaggaaaa 1440aagagcccaa ttcagtgtct ttcacagcgt gttaaataaa agcacacatc aaccttcctt 1500tccatcttga ggtccttccc tatcccaggt ggggaattca ctgttctgaa ttgtccatgg 1560accaataatc aggtaaaaaa ataagttgca atccttgcaa atctaaaaat gcatacaaag 1620accaccacta acgaaatata ttgctactca cacagtccgg aaagacacag taaaataatc 1680tattccttag aacagcggaa tgacaaatta cttgcaatga aataaagcat gatcactcac 1740ttcaaaagaa tttacttcaa tatcctcaaa ttttcctgta actgatgttc ctgcacagtt 1800gacaagcagg tcaactggtc ccagcttctc ctgagcctgc aagaggacag aaattaagct 1860ctccagcagg aaaaaaagca ttcatatagc atagtgtcat cattaaatat actgccactt 1920gaaacacatc gtacagagat gaccttaata catacgctgg tggaacagtt gcttctcacc 1980tgttttagaa cgttctccac ctgttcatag tctttagata catcaacaga aatacacagc 2040acaacctaaa aatggaaaat gaaagaacat attataacct cagtccaaga atggttggcc 2100ccaagtctta caaaagaaac tgcacactgc ttcatgaggt catattaggg acaaaattag 2160aaaaaaacaa gactaaaaaa aactgattag tggcacagag ctaaatatgc ctaaaaagtg 2220atttaacgtt tactctttgt ctgggaacaa gaggttatgg atcttagttt gaacgataca 2280ggaaaatgaa aatagacact ggcccagggg agaataaata aataaatagt aaaaaaaaga 2340gagacagcaa tgtcttaatt tttgttgcag ctggaacgta ggtattgatt ttctttaaaa 2400ctgctgatta catttagcaa cttgatctca gaatctaagt tttgaagcac cttccagtca 2460acttccagcc aaaggaagca ctgactttgg ggtatcacca actgtaagat cactgccaac 2520tccagcttgg ccggggattt acaaagagat tatcccctct gctaaacaac tatcaaggtt 2580ctgaggcaac tcagtatctt gaaaggagaa gcaatcacat accacaatag aagtagagac 2640tcctgtattc tcattctgat ttctacgtct tactttgtcc aattccctta aagctggttt 2700gaggtgaaat aaagtcatca ccggattcta acaagcgtca tcagggtcac ttgtcatgaa 2760gcacgccaac aaaaatgaag aaaagcttac caaagtgtaa ctaactgctt tggcagctag 2820tcatttgtca gttttgcctc tggtcagact gcaacacttc ccagctacat aaacagaatc 2880tgcttgccat tcctgtgcag caggttggct attcctgcct tccagcactg tgcatagctg 2940gacatcggag taaacttgtg gtgcaagtta atggttggcc agcaagcatc tccccttagg 3000gcatttttag attttagaaa tatttatctt ttacataagt ttgagaacaa aaataacagc 3060tgagctaaga taacactgat ttactatctg gatgtcttta caagatcagc aaggttaaaa 3120atcacagttc catacgaaaa ttggacatcg gaccacagat cagttaatca tactgagaac 3180aatatactca ggaactacta aagcttgtat tggctaccag cacattatac aattcatctt 3240tttgcttcta ttattgtatt tcctttctgc tacattaagt tgattacctc cagtccagag 3300catgcactgt gaatgtggtc ctaataaaca gactatgctg ccaggaagtc taatatcctc 3360attccagttt ctcgtctttt gttctaatag cgcattatct gaccattcct aaagcattcg 3420tttctcaata aaagctcaac tccactccca gtgaagtaac taggaatatt ccatactgag 3480aaagtgattt taaccatttt ccaaaaaaat tatgggcaag catttcattt cactgcatcc 3540ctttatttgt aaaggctgca ctgtcagcat tctaaataaa tcacatacaa atgtatttca 3600gagaacacgt gccaaatcca acacctaaag gtacgttgac accagaatcc tggttctcaa 3660tgcaacttag tttgacatat atatacatga gcagaatgca cagcatataa tcatgtaaaa 3720actggtattt cctctggatt tttgctccct agcttggcat cttgcaaatg taacagcctc 3780tgtggtctgg caacccatcc cagacaactc tatactttca gcaatcttct tgtacagcct 3840ctgtctcttt cgctgccctc atttgtcagc ttcttatctg ccgttcttct tctatttgtg 3900ttctttgaat ttagaacagg cagacttccc tgtgacaatg actacaactc aacagtaggt 3960acacgagggc actcccacta cagccagatg aagtatggac aagtctaaat ttgctactgc 4020taaaacaatc ctgtttagct cacactgaag cttgttcagt cttgaactta ctgaaatttt 4080aactgaaact cttgcagaca catacctagt actcttctta agaaaacaga aatgtaagtc 4140cacatctgtc aagcttggac agatttttaa caagagcatt gtgacagtgt ttgacaaagg 4200atcaaagtca ttgtcaaccc tgggaagaga ctagagcaac taaagggaaa cccttactgg 4260ggtttatttg tttttaaaag gggacaaaac gttgctcctc ctcacctcac atgacttcat 4320atttcaagat aaatttgatc tgagtaaggc aacatgcaca gaaagaaaga tcaccttgaa 4380ccatttgaca ggaatgagca aagacagaat cttgctgagt gttcttgata tcttcattta 4440catcggctag tactaacatt gcttccaaat atccatcttt ctccactgct tcttttcagg 4500agaatatcca acttatccat tcaccagatt attatacacc caatagacac caaatccctt 4560atcacaaaca aattgcttgc acttgtaata aagaaaataa gaaccaacta ctggagcaca 4620agagagagga aatcaaagat gcgatggtct caatgctgtt tcccgtctct tttggattat 4680gagtattaag aaaaaaaagt tgtagtattt catcttgaag ctgtgttttt ttgaacacag 4740aaagctgccc tattttaaaa catagcaccc cagcaagcat taatgcactc aaacagtgca 4800ttaagcgtga atacattatt tatttaccaa agtacctcca gtgtttagaa acctcagtgc 4860tttgccagga aaacagcaat acacagcaca acatagggaa tgaaatctga aaataagtac 4920ctaaaattta ttccccatta caattgctgc atttacgatc tctcgaagag aaacttgaaa 4980aaagtaggtt attctcctca atcttgaaca atacaaacac ttatcaagtg acagcaaatt 5040gcagctatca aaaaaaactc taatacacct acaggtgaag ataccaatct ttttctctgg 5100gaaaaaaagt tagcatacta accaggcaac atcaaatgta gctgagtttg gaacatcaca 5160cgtagacaga ttggaatttt ttgaattcaa gtgaggcctc acctattttt aatcaaacag 5220tttggtttta aaaaccagac tactgaagct atgttgaagc gactaccttg aaacacaaca 5280gttataggta gcagcgatct tacctgcttg tcattaacag agtacttttc tatttccttc 5340tttgtctgta acagcttgtt ctgaaacgga taataagaac agctaaagca ccgcagttat 5400gagaacacat ttgtcttcag tatttcgaga agccctagaa acactctcta aaaacaagaa 5460cattatcatc agctagcgat aacgctttac tatgttcatc ttagaggttt atgaacgtac 5520aaaacatcaa ctccatgcca tagcaatgga agagttttaa ggcaagctca tcacaaactg 5580catgttagat tacactactt acaaaggcag ccatctctac tctgcaaata catcacattt 5640aaattattag gatcacctta aagcatacag tggaaaaaaa accaatgagt aattatcaat 5700tcccaaatta ctcctcaacc ataccaattt cattaatata aggtagccag caaaaataca 5760aatacgctta cctcatctct tgcaatcagt gttatgaaag ctccttgctt ataacattca 5820atagcaatac attttccaat tccactggag cctccagtaa cctggaagtt atttgaaaag 5880tcagttagtc cgttattctc atccctaccc ctgtatcata acaagtcaaa tcaaaactct 5940aaaagtgtac tgagattcaa atatcaccca gtgaagtatg aacacatgag atatacaaaa 6000gtttctttac ttactgagta cgaatggaaa aatgaaatct aactaccttg tcaggcaact 6060ctctctgtcc caaaaaccag ctttacctta atggtatttt ttccatattt cttatgtcag 6120gctcacttag acccactctc acgtgccttt gattaactta tttttgaagt acggtaatag 6180ttataagttt gcaatcacta ttaacattaa gaacgcaatt acatcctgtc cctccatgcc 6240aggctacaat aaaatgcata aaaagagcaa tcatagcttg tctgccaaaa acaccgtgta 6300aacaaacaca atttaaaaag ttatcttctt tcttattaaa taaacatcct taatttgaca 6360gctggccccc tgcagagcac gtctcacagt tcacatctgg atgctgaact gcctcaatgc 6420ccttctgtgg ggatcccagt tctaagcctc cataagcctt tccttcttcc tctaaaccat 6480aactctcagt ggtctcccat gcaaacggga aataaacagt ttgcctgcag acaaatgcaa 6540ggcgatggct acaacccccc actaataaac aggttaaagt cacctgaaca cccccctctc 6600taccctccag aaaaatctat ttgtgaactc catttaaaac aaaacataga tggtgaccca 6660actgctcttc ttcttatcgc aaataaaact gtcatcctga tactgacttt tggtgatttg 6720ttcatttaaa cccagcctca gtttgtacgt gccttcagtc cttaaacaca cagagttgta 6780ttttccttgt atggccaaat gtgttgaatt ttctcctaag agcgtatcac tgagaaaaga 6840ttggaatgtt tcattgatga agggagttct gaaatacagg cttaaatatt tgagaaagag 6900attcatattc ctctggaagt tgtcatttct gttctttatc aagacttcag agaaagtaag 6960gtatcacctc tatttcaact atgagaaatg gaatgcactg agccaatcag tggaagagca 7020aaggcttcca gtctcatctg cacgtgcctt tgttttgttt tcaggctcac taccctcaaa 7080attctttcat attcctgggg gcagtaaagt caaaagagtt agaagtacgc tcccatgagt 7140caccaagctc ttctttctta cgagtgtttc atcacaaacc ttcaaagaaa acagctcagt 7200acacaaaggg tgatcatgta acctcagcaa acttattttc aacagattcc acagtttttg 7260tctaatttgt ctgctgtcac cagtgggtca agaacttaga aagactaact tcaacttaaa 7320cagagtttca tagacttccc ctattagaga aagtcgcttc aaagcagctt ctaatcgact 7380ctttactacg ctaacctttg tgaatgactt taagatgcac gttctgttcc ggagatgttt 7440aatgctgttg cattctgaat aaaggatggc tgcttcctaa gcaaattaag tctgtaagaa 7500cgagtgttaa cgtttgggaa ccctagcatc atttaattct tcacaacaga aagaagttaa 7560atttttagtt cattcccaaa gcacttttga agattccaat acatctctag aaggattagg 7620agattcagtt tgatgtattc aggcaaacct cagttccaga gcactcatga gctgccttta 7680cagattttca gcagcagctg gtacatcagc acaggaacta atgaatgcat gtggaagact 7740gctccaattc ccatttttgg cacagcactg aggcacagct gattgctacc tgtacaaatt 7800gctaagtgtc atgaacaggt caactacagc agtgtttgaa ggtgaaggtg gcataatgcc 7860tatggcttgt tagacaacgt taacgttcac ttcggaaaga caaggtctga agtcaagcaa 7920gaaagggaag tttttatttg cagtcatacc caagaacgct agatagggct catgtcagat 7980tggtcagtga caaaaagcag aatcagttag ctaggaactt cagcatttag gtggaaggct 8040gttactaata cgttggaggt tgttactaac atgcaccacg agatgcaatg taacagtaaa 8100gaaacagaac aactcatcga tcaatgagta agcacaattc caaatcatca caactatcat 8160cagaagcgat caaaatgaag acttgatatg ctctttccaa cagggcttgc tagctttcac 8220tgagcagcag cacaggatgc aagctggctt cttgctttta gacagtactc taacagtagg 8280cattcagttc cactgaaaca gaacatcccc gtaacttgct ccaattggct ggtgtgcctg 8340actggactcc aaatgacctc ttcctgaacc acccatgtgc atttaagcca caggtcacgc 8400tgctcccaat gcaagagcta caaagctgag acacggagat gggtcaactc caggaacacc 8460caaagcctgc tgtccttatc tgatgggctc tgcagggaaa tgctgaaact actcctagtc 8520ctgagatggc ccttacacct gtggcttggg catactgaag cttaactatg tccaagttaa 8580aggccctgct caaactgaaa ggaagacaat cacaggcagc aaatatcctt acattcctgg 8640catagttagc ctatgaaagc aaaaatacat ttaaaagcat tttgttttac taaataagca 8700aaaactgatg cttgagtcaa cctgatagct gtgaagtcct tcctatttaa cagtgcccac 8760tgagattacc aaaacacaca gcagatatgg aacaggaaaa cacccccaca agattagatc 8820agtaatttgg agttctgtgg agctccattc ctctctaagc agaagagaca cctgtggtct 8880cggtcccact ctaaagcata cctcatgtca cacagcaagt gactccagga gaggacagga 8940gggaaaaaaa ccccaacaaa aaaacaacca cacattttct gcctcagatt tctgtccttg 9000tacctcaaac acctagctct attaaccatt acaactgtta ggagcagaga gaaatagatt 9060ctacaaatga ttaagtaggg tgtgaaaaaa tgctgattta aattgagaat tcaacaagta 9120actgctagga aacaatatac acagtagcgt ccaaacacat tcaatgaccc tctttaagaa 9180atactacagg tattctcctg cacatatgaa gggggaatta tacatacaga tcacacaaac 9240agattttgac tttttctgtg aaaacacttc aaggaagttc ttaacagtgc tacggtcctg 9300aatattttca tgacaccttc tgataatttc agtagcagag aacactcact cccattatac 9360gatgcaagaa ttcaccttgc acatgtgatg tgtacagcct tcacaagtac agtttctaat 9420ttacaaaact atcatccaat gtttttcctt acacaattca cccaaacatt tgaaacccaa 9480acatgcaagt ctaacagcat cagggttatt agcagcacct acatatcaaa aaaactaaaa 9540ctctgaaaca actgctccat gtttcatgtg tttgcacaaa tgctttcaga gcataactga 9600agccatagtc aaacaagtgc ttctgccaga agcaatgcca ctgaagctga tcacccacag 9660atttgtaccc tgatgccaat aaatacctac agcctttcct tctattggga tatgaacaca 9720gttaatcctc cagaaggtgg tgacgcactg gaacaggttg cccaaggaag ttgtagatgc 9780cccatccctg gaggcattca aggccaggct ggatgtggct ctgggcagac tggcctactg 9840gttggcgacc ctgcacacag caggggggtt gaaactagat gctcattgtg gtccatttca 9900acccaggcca ttctataatc ctacgaatag tcattttgag accatcactt atgtcaaatt 9960caggttacgt ggctaataca attagcagta gtggctgtga gggaagattt ctccaacaag 10020attattcttt gtcattttca ttgtgagcca actgaagtgg ctctttgaaa aaagaagaac 10080cagcagagta gctttggaaa aagcgtaacg acacaaagaa aagacaacac tcgggataat 10140cagattaaaa acaaacaggt ggacaatact ctgggataga atacactgaa cattttgttg 10200cttactattc tgtttccacg caagcactgc agtaccctta ccctgcttca cctttgcttt 10260tacacagtac agaaggattc ctgcttagca agagttaccg ctgtgggaag aacctcagag 10320agccttcact cacgctctac tatctccagc aggacatgat gctgtaaagc cagttacaat 10380acccagcaat acctattgca tcaagtaatt tgggaacact gttgcaactt ggacagctcc 10440aagccgggac agctctatcc gcaaagagca gccctaaaac aaataggcag ataaaaatga 10500acacaaacaa acaaggcacc acacagagct caaaaaaatc ccaaatgcca agcagctgtc 10560aattccctcg cacctcagag gtctaacttc tgcattacac ccaagtcctg tagtaaccaa 10620gtcctgtagg cagcctgcat gccctactca ccccccaaaa gcagattcag caagcaaaca 10680gcacagctcc tctacacgga gcacaccacg ggtaactaca gctaagtccc cagagctgac 10740tgaaggacca cagccgcccc gacccgctct cacgcaccca catgctccac acacacctgc 10800aggcccctgg ctgctgctcc ccatcacgca cccgcccagc tttcagcggt acgctcctgg 10860ggaaccgttc aaaagctata ttttcccgaa taaaccctcc caaaggctcg ctcctacaca 10920gctgattaca gacaagccaa acgtcgctcg tggacacgga tacccgcgct gagtgccgcc 10980tgaccgcttc ccttcgcgtc agcccgcccg tttcctcagc acgggtcgcc tttcagcccg 11040tgccttccac cctcgtgagg agggccgcac tccagcacct cggcagatgc agcggggcct 11100tccccgggac acggcggccg cggcctcccc gctccctcct cccgcccagc gccggcagcg 11160gaccgctccc ccgcggtcgg tcagccagca gcggccggga tcggggtggg gagggggagg 11220cgagaggcct cgttcgactc accacgacgt gtgcgccggg cagcttgagg ggcttggggc 11280tgatgagcgg cgacaccatg tagaggagaa ggacgatggc cacgatgaag gcggccgcca 11340ggagcagcat ggctcgggcc gggcttgccg ctggggaggg ggcggcgggc ggtgacaagg 11400cccctggggc tgcgggaggc gccgagcgcg gcgcggcccg gcgcggcacg gacagcggga 11460gtagaaccgg tgcccgcctg cgccgcggcg ccacggggcc acaggggagg gggaggagga 11520agaggaggag ggaggaggag aacacggccg ccactccgcg ccctgattgg ctggtgggcg 11580gggcgggcgc ggcctcgcga gcggggattg gccagtgagc gacgggaagg agctggcgga 11640ttggccgaga ggcgggacgg cgctcggaga ttggagccgc aggctgtttg cagggtcacc 11700gttggaggca aagggcggcg gagaagagag agttcctccg gagaagacgg ggtgcgggac 11760ggcgccccct acggtccccg cctagggcgg gtgaggcgag gagtgaggta ggccccgccc 11820cttctcgtca cgtggggctt cccgccgaaa ggagggggcg tggctccgtg aggtgagaga 11880cgggaggctg cgggcggcgt tcacgctctg gaacgcgagg gcagctgttt gtggggaaaa 11940aaatgtgtag aagcgtggtt tcaaagcata ataataaaag attaactaaa acgaaaacgt 12000cttgcagctc aaataaaatg attccgtgcc tacgttcaat atttccttcg ctgtttatga 12060ctgaaaggaa ctccctgaaa tgatttatgt tgtaaacgct gtgcagcctc tgacttgtag 12120aagggagttt gcagcgtacg cggctttacg gcctcggaga gattacgatt acgggcgaga 12180gggcgtgcgg aagggtgtga agataatgca ggagatgaga ttggagcggg gagtaggcgg 12240aaatgggagc agggctgcgg gcagggatta ggcagtcgtc aaggggagag caaagaatct 12300ggaacaaaag aatccaggaa ttagttctgg aatgggattg agccgggatc gggcttcggt 12360gacgctttga gagtggtgct gtggggtgga ggtgtgagga aatgagagga agaaagagcg 12420cgtgctgagg taacagctgc cacggcaagg gtggggagag agctgacaaa gtggtgtgtc 12480caaggaaagg cggtgtggaa tcgtaggcat ccttaaggct ggaaagggtc accaagtcca 12540agcaccaagt ccaactagca gaagttggtg taggatatgg actaggaacg ctgcaagcac 12600agataccgac ttcattcttt gcatacaggg cagtgtatgt gttatctttt gtagaatatt 12660aattaaacac aaaggaggag attgataatg taatagagcc tatttatagt tatctagtgc 12720agaatatggc gagacttgaa aagcccaaat gtcagcagca tggagataaa gcagacggag 12780ataaatccat ctttcacaat gcgatatcgc tttcagaatc aacatgaggc aagggcatgg 12840ataaaaacaa tcatctgcag ttaatttcta gtaaaatgaa ggttaaacat gttggtaggg 12900ggcctctaaa aacctcaaat gcatgatatg ctcctgattg gtcacagtta ggatcacata 12960ttactaaata tttgagaagc ccttgtagat taacgaggaa tcccctcggt gaattttatg 13020cagaaatcca tactgtcttt tccttttagc taagtggcca ctttacaacc gtgtgattga 13080caatccaggt agcgtccact cacattttgt tcctggggca gtgaagtgtc atgaatttat 13140ctccaagaaa aacattcaaa agtgaagacc ttgtgaactg cttataactc accaatgtat 13200cgccacagca gtaggttttt gactcttttt aggtatgcca gcaggcactg aagtttgccc 13260tcctgagctg tctgctgtct ggtttgtatt tgtctcatgt gacctcattc actgaggaag 13320tgcgttcctg acacacggga atggtttgct acgaaactct tttctcagtg actgtggaac 13380tggaaattga accctaaaaa aaaaaagtgt tgaagccctc cagtccaaac tttggttgta 13440cataaagcag tatttaatta atctgacctt gattaacaac atcaaaaagt gtaattttga 13500agcacaaact gaccaaggta tgtatgtacc ttcgggatgg gtaagaaaat aaaaaggtta 13560acacatgcta attgctttgc taattaatcc ttagaagcag cttcaacaca acagcgatgt 13620gtttagagaa gaaaatcaaa tacaggtaga ttaaagcgtc caaactatag gaccagctgt 13680ggttttctgc ttcctcagtt ctgttcatat aatctttcaa cagacgtttg cagtaacaat 13740gttgtgggtt gagataaatc agtatgaaca aagcatggca accgaagtaa gaaagtagtc 13800atttaaacac ggaaacaaat gtatgaattg ataatattac aacacaagtg actgatacta 13860gaggtgtcct tttgatcttc ttgttcccaa agcatacaag gtacacacag aagagacaca 13920ggctgtgtta agatgccatt aagagaaggc ataaaggttt gacagagcag gtagtgaggt 13980tgcagcctgg acagactttc ttattgcact tgagtactca tctgctggat tttctggttg 14040tgtcatattc acgttaggga gagaggaggg aaaaagagca ggatgcgtag gctactcagt 14100gattaaacaa aaaaaaaaag ctggaaactt cttcatgtga tttccatcca gtcagtcctt 14160ctgcttttag agaaagcagc atgaaggaaa aacttcagta gccaaggaga acaacttttt 14220ccttctgttt tcctgaatta acttactttc ctctccaacc ttctcccttt tgtgtagcaa 14280gcataggtgt tctatgctca tttcttaaga ggtctgttgc agtaatcatc ataagacatc 14340aaaggcatgt tggcagttct tggattcctg caaagcttca agatttagaa tgatggcagt 14400ctaggtgagt tgttcctggt caacaagctg tcttgatccc gtgtcccaaa tgagaagagc 14460taatagggac ataagaactg aaatcagaaa aggatttaca taacatgctg gcagtagagg 14520agaattgggc aagaaataat gatctgcaca tggtagtgac taaagcagtg tgactgaaat 14580acttatcaca cccagctgct tgccttgctg ttcttcccca aacaaacaag caaatccctt 14640gtagctgaac aatagcttct ttactggtcc atcacgctgg agagatcatc agctacccca 14700tgcatagcag ggtgaaacag ctcccagagc actgtgcagg tcaaagtact atatgtaccc 14760tgtctgctgg agtgctatca cggtgatctt ctgggtattc ctagaaggag atttcctgta 14820ctcccaagct caacgtatca tccagaaagt gctcgcctgc agcagggacg ggttctggcg 14880atctctgcag cttccagcta tgccgcatgc ccttatcgca atgaactcag gctgggctga 14940tggcccaggt gctggaggct gccagcacgc aggcaggagg tggttatagc agctcaggct 15000caggtcaaac caaggcttct tgctggggca gaggggactg

actctgtggt gcaaaagcag 15060gtagtatata tatatgtata tatatacaaa gcccagctac cagctgagag tcccaaggct 15120gctgcagtag ttttgcaatg agcacacagg aaacaagaag atcgctgaga acactgctga 15180aatcagattt ctgtcttcac acaggtcaag ctgatttaac tgtttaatgt aattgctgca 15240gttgcttgga aaaaaaaaga aatagtaaaa ccatgtccaa aatgaaccat tcataactgg 15300tggcccatta tgtgtcacag ccgatgttgt gctgaataaa taactgtaca ggtattttat 15360atattgagca acatatttat tgaaacaaaa ataatttacc tcaaaccagc ggtaaaagga 15420agtctttact gtctaattta aataggcata agttaaactc gggactgaga tgatcttgaa 15480tttcatttgg tgcccatggt tctttttatg tggtacacct gcttacactt accatcacac 15540tggagcagtt tgcttttgcc acccgaatgt cagacactgc tatagattta cagtagcttg 15600ggggggctgc aggttggaag agggggttga ggcctcatca agtgccatgg caaaacaccc 15660tcaagtaagc acggctggaa gcaggaagga tgagggaatg agctgccatt tcctttgcgc 15720tggaaggatc actgctaaaa cttgtaaata tctgttagaa acaaacaggg acgttcactt 15780tgtcctgtga tgcaagagca cccattctga atttttatct cctgcaaagt tgtatttaag 15840ctgatgttta ccgtggacgt tcgtgttaca agatagcctt tgatactatc aataacaagt 15900cctctttgat gaagtaaagc tacagagtca caaagcatgc acttgtctga ccctttgcct 15960ggctgcctgt ccaaccacgt tgcaccacta cacccagccc cacgagacct gctccagggc 16020caagggaatt gagcacttaa gggaaagtgc tttgtacaaa acatggcgct tatgagtttg 16080aaaacgtaga tccaccaaaa cctcctcagg cacgatgagt atattttttc tccactactt 16140acagcgctgt gaattctagt taagggcgtt ttgattccta aagaattttt ccttctaatc 16200atagacgtac tccagtcctt attccagaag gcttactcct tgtattttga aggtcttatc 16260ctgaaattgg gatgcagagc cattctgaaa atgacagtat tttaagactt tgctgcactt 16320actctggctt cccacatacc ttcctcttgc aaccttccac ctcccagaac tgcagcccag 16380cctatcctcc tctgccagaa aatcggatcc cacaggccct atctcacacc tcccggttcc 16440ccatcctcat ggcagctgcc ctctttccca aggcactcta tggagcagca gaactgctga 16500gtgcacaggg caaagatctg ccgttccgag agagcagaga agcatcgctc gggaatcact 16560gcactgctgc agcactattg tattctgcct ttattcagag gcagtccttc acctatgaat 16620atcactacta ccttactgaa tatatatttt caggaatatt ttcacttttt agccagatag 16680gaagcggatt ttgtaattac ccttccagca acttacagcc aattactgtc tctcctcctg 16740attcctgtcc agcaatttgg ttgcagttat tgcttctcca gagcgggcag aattttttgc 16800tttaggaaat gtacacctcg aggtaatctt tgaagagtga caggttctaa agttcacaag 16860tttgatctgc tttgggatta agctacctgc taaactacca cacgccatcc agtcaagcca 16920tttctattat gtgcgtatgg ctgattctta tcacaaaaga tcaagttaat gatttgcagt 16980cttcggcaag cctctggttt ctttgaactt gctttttgta agcgatattc tcgggtactt 17040tttgtgcttg tgaagctact gcagtgctct ggagattttc tttgtgctcc tggctgtcag 17100agttatccat ttctaggcct gcttggccat ccccatagca cggggagaac cgtactttcc 17160cattgccctt gtacctgcac ttgtaaaaac gctagaggaa ctgaaattac ttcaagttcg 17220tgccctgtcc tctttcaaag ccattctgag aactttcttt gcacaacctt tttacaagag 17280ttaaatccgt ttctagttcc aggcaacaca cttgtcatac acagcgctgg caagggactg 17340ctgtttattt cttgcttgga tgcaattaca cagccatgtg cccttgtttt cagtccctga 17400tccattatct ttggcattta ctgcaaagaa gctgctgtta cgcaatggaa atttagatga 17460tctctttttc ttagcttact tctcctctaa cccaagaaat gagtacagta tagcctgctg 17520aatgcaagga aacctgcacc tgcaaacttt tctccccact gcgtcactac caaatatgtc 17580agagttgctt gtacttctta agtctgtttc catcccctaa tggcacgaac cgttgccctc 17640ctgttgtcag actgcaaaaa ggccagcttg tacagatttg ccctgtaggt ttgaatggaa 17700gaagggaaaa aaatcagaga aactgccagc ttttgttctg ccgcttgtaa gcttgctttg 17760gtagaaaagt tgaagaaata ggaacatgct ttgaaatagg attttaaaag gaatcagctt 17820cttatcttcc ctttgggaaa aaatagtgtg aaggacagaa taaatcagac ggaaaaagaa 17880agaaattgac gtaagagaac tagtcgggca gaaaggagga ggtggaaaat acccaaaagc 17940agcaggaaag agggaggcac aggttgccaa ttaacacttc gatcaaagga aaggcccgat 18000caaaaccttt ttcctcctct aagaagcatc accccttccc actgcttact gcaatgaagc 18060gagcttttag actaagactc aagagaataa ccccaatacc agtaaagcct gcagaacttg 18120tttttttcat agctgacacc acagacaaac aaacaaataa ataaatagta gcgcagagca 18180tcagcaccgt ggcagtcatt ccagcaatca cttccccacc gtgctctcct ataggagagc 18240tgcagcacag gtcagcgtct cccaacccgt gcacttcttc acggacagat ttgcatcatg 18300cagaccctca gattgcccag gaagaacaga actgcaatgc ccagaaagag tgtggaagct 18360ctgagaattt atctgcctgc tggacagagc ccatctacac ctggaacaag cgggcacctc 18420tctgtgctac cagtgctggg taaagaaagc tgtgcagcag ctcctccctg aacactggct 18480acgttgtgac atcagccctg tggttcctgt ggcagctcct gcgcttctgc aactacatga 18540gtctagctgg caggccacct gcttgtttcc atatcagcag cagccacgtg caccatgtgc 18600accatgtgca gggggcctcc aggcaggtaa aaaaaacaaa caaacaaaaa catctcttaa 18660ttacaggggc agagcagggc tggatacgaa caaacaaaac cataccaaaa caagcacacg 18720tgtaaagagg aaaaaaaaat aaaataaatc acagctttgc agtttgtctg tcttcagagc 18780aaatcaaggc tgtgattaat tcgttacaca tcagaactcc aagcaggctc aagctgagcc 18840gttgcaactg gcattatgaa tggcacactt gaaaaacagc caggttgctt tccagattca 18900tggaatcata tcatagaatc atagaacggc ctgggttgaa aaggaccaca atgatcatcg 18960agtttcaacc cccctgctac gtgcagggtc gccaaccacc agaccaggct gcccagagcc 19020acatccagcc tggccttgaa tgcctccagg gatggggcat ccacaacctc cttgggcaac 19080ctgttccaag atgttagctt ctctaacatc ttaccacaac ataataatga aagaatattt 19140aaaaaatccg tgatgggtag gaacttcctg gctgcagcct gtgctccagc cctcaggtgg 19200tggaaggaaa taatcatttc tagttggaat tttcattttc tttttttttt cctcagcttt 19260caagtaggca aacaattcac ttgtcttcca gagctcaaat cactgctgta agtaacagtt 19320ttcatttgtc atttttattt cctctgtgag atggtgatat ttatagcaac attctcggtc 19380ccttgcttgg atgacttgtg attgctacgg ttcttgtaac agcattgcca gaacagtagc 19440aaaaggcaac tgctccagca ccggtttttg taagccatta cctgtagaca ctcatctgcc 19500tacagtagta tgagtcagtg gaaattactg tttatagttt acagaccaca tgtgacaccg 19560agcatgtttg aaagcaaagt ccctgccttg aatagctgag atttaaatta gctgaggcag 19620cagaggagga gggagggcaa gcaaaagcag gtcttgccaa tccatggcat ggtgcctagt 19680gataggtcac caagcaggaa agaaaaccca accctggctt cattatcaac atcaggccta 19740tgctcaggtg cccgtgactt atttcctgag aagtctcaaa acacgaccaa cacctgtttg 19800aactcctata agagagctta gcgcctgcta tgatgcaggt aggatactga tgtttatttt 19860cattactagt gcgtgacaca tccaaagaaa ttagctgtaa aatgtctagt attcctgcaa 19920aagaacgtaa cagatcctgc acgtggcagg taccatgcac agatggcacc aacggatgga 19980tgctggcttc ctcacacgtt gagttgttgt ggagttgctc tgatgaaggg gagcagcatt 20040tgtgagcatt cattcatgga gctggagtct cctaagcaag gtaacgaatg caaaggtggg 20100agtgttcaag tggcctaggc aggcttgggc agtgagccca ggtgaacctc atgaagtcca 20160acagaaccaa atgcaagatc tggcctctgc attgaggcag ctcccactac caatacaagc 20220tgggaaagga ctgagtgcag ccctgctgag gaagacctgg ggggtattgg tggttgggaa 20280gctggacatg agccagcaat gtaccctcac agcccagaaa gccgactgta tcctgggctg 20340catcaaaagt agtgtggcca gcacagcaag ggaggtgctc ctgcccctct actctgtgct 20400ggtgaggcct cacctggagt actgcatcca gatgcggagt cctcagtaca ggagagacat 20460ggacctgttg gagcgcatcc agaagaggac cacagaaatg ttctatggaa tgggacacct 20520ctcttacaag aacaggctga gagagctggg gctgttcagc ctggagaaga gaaggctgtg 20580agttgacctg atagcaacct ggcagtatct aaagggcagc tacaggaaag aagggaacag 20640actttttaag cagggtctgg tgtgatagga gaaggggaaa tggtttcaag ctcaaagagg 20700gaagatttaa gttagatata aggataaaat tttttacagt gaggatggtg aggcactgga 20760acccagcgtt gtggttgaag ccctgacccc tgagactttc aaggcgaggc tggctcaggc 20820cctgggcacc ctgatctagc tgtggtgtcc ctacgcactg caggggagtt gaactagatg 20880gccttcagag gtcccttcca actgcaaaga ttctgtgatt ctagtaaaca gaaagcgtac 20940agaacagtga cctagtcaaa aattgactat cggaagggcg tgtgggtaga ggtaggcagg 21000caaaactgta attaggtcaa agaaaaatga caggacaagc ttatctaata tttgggatgt 21060cagtagccaa atgccagtac agaggatgaa cagcaaccat taagaatttt ttacacaggt 21120aattctgaca acagagaatt tggggagtaa taattgaaat attattggta aaacggtatt 21180tttaaagaaa aatcaaggtg agagcacaat agctacaaca tagactaccc gctcaagaat 21240agaaggagca atgttttgat aataataaag tagctgttgg aaaagcagca aaattggaag 21300caaacagtcc atcaagtgct tgcaataggt tatgtaagtt gtgtgaatgg ctctaagtca 21360gccatgatta ctaggatgaa tctggttaag acaaacatgt atggaagcca accatgaaac 21420cacggtcatc attctggagg aaggaagaat tatgcagcaa aatcaaggca ttcctgcata 21480tttcaataat tcagagctat taaaaagctc cctgtcacga taatcttcag aaataatgtg 21540aaaaaaatac atagcggagc aaattttcat taggaagaca actaaataaa cacaaaaagt 21600agatcaaaca atggctcaac agaatattta aagcagtttc tttgcttcag ctgccaaaga 21660gcaaactacg atcaggtgca gctgactgat aggagcacaa aagctgattc aagggtatct 21720gcccagacga cgtgtcgaca tgttctgctc cactcattaa aacaaaagca gtcaactcaa 21780ctctgaaggc tagtagttga ataataataa taaaatcaaa accaaacaaa ccttaccaat 21840ctctaagaca gacaaaacca gtacttaaac cagggaaggg acagaactct ggattcagag 21900attaatcagg tgacgtgggc aaagatacag ccagagaatt taatgagttt ttctaactct 21960atgaaaatat gtgttgagaa aatccactgt tagtcaatgg gaagaaacat ctgtgaagaa 22020caaagcaagc aagcacagac acaactgatt taaaaactat tttacccaca gaacataatt 22080tttcatactg cagtcagagg tagcaatagc actagaagtt aggaaaaaaa cgtaggccaa 22140gtagtcaaag actagtcaca gctggcagca tgaaagatat gcaagtaatt tatccagtgc 22200ttagaggctg tggttatata aagcaaatat aacctttaat caactataaa ccaggcagca 22260ttgtttagag tacataggtt gctctgaaag taacgcctcc tatttatttc cacagaaact 22320acaactgata cgaagagcat aacaacactg atagagcata ttcccagcta caaaacacta 22380cttttcaact cagtcatcac gattagctct gcatttttgc cagcgatgag tgagagcctg 22440catgctacgc gcacagaaat ctgcaccagt ggaggtgccc caccgtcact ggtgctgaaa 22500tgcaccaccc actgcctcac cgcgctcaca tccactgctt ggtctccata aatattcagc 22560aagcattgat gaatgtcaag aggtgtaatt ttttctgtgt ggaggaattc agtgacacct 22620ctgcttcatt tgcgcttcca gccaaatgcc attctgtcat gctgcctctc tgctgccatc 22680tgtcgcacag ccagagcaca taatggaata ctggtaggaa ggttcaacca ctaccgccat 22740accaccaatg acaccttggg ctgatgatat actaaaataa atactacctt tggggtagca 22800ctcatagttt aggttaaccc tgaaaactga agtaaatgac acctccccct acctcccgca 22860gccatttagc tacatcttct gggataactt agctaggaat ctgtgataat atttcaactt 22920atcctcattg tagaaaaaca gcagaacaaa aggttatcct tagagctgca gttctaaccg 22980gcagtgttta tttggaatat ttctaaaaac aacttgaaac accaaacatt aatgtttcca 23040tttccatgag caagtagcga gatgcaagtt taaaacatac agtatatttt tctacgttaa 23100agataaggga ttacacagtt aggtttaggg aaacccatag gacaaactga cctacgacaa 23160caacaaagaa acgcatttcc tgagaatttt aagattgcca taaggactgt catataggac 23220tgttataaag gtcaattaac aagtaattca ggcagtagct tcaatcctcc aggtgagagc 23280cctgccagtg cgtggctcgc ttctgaagtg ttcaccagag gcaacagagc aaagaatcct 23340gctgcaacta agatcaagtt tacaaaccac agtaacttgc atctacactt gaatttcccc 23400cgcttgccca caaaggtcca caaaaagatt tgcagccccc tgaatcacat tcacattttc 23460cagtgcgaga ccgaagtaaa gctgcaaaac tgaatgactt tggaaagaac atttcattat 23520gttagcaaac aaaagctcag caccttgcag atcaaagaat ttgtatttaa gtgttttgtt 23580tagctgtcaa acgtagaaac aaaagtctaa acaaaagtga tagttttgaa agtaacactg 23640aagaaatact caagaacata actgatgttg tacattttac ttcatttaag tacagcaaat 23700ttcacccatc ctatgattta tcgagtacgc aaaatatgta catagaggaa accaaaaccc 23760ataaaaagac aatcatctat gtgcatatgc gcatgtaaca tatgcacatg aaatgtgcaa 23820ttttctttta atgcaagtta aacaaagcat atgcacaaca gagttgcaca accattacag 23880aacagagtgt tctgggtatc ctcatgatgt ttcgcatcta cagccagtgc aaacttacaa 23940ggcacaaact cagtgctgac accgtagtgt tgtaagttca ggcacatttc aatttgtagt 24000tcttaaagat aataatcaac agaagtgcta cttctgtact aaagtgccag cctcttccca 24060aagattaagc attaagttga tgtaacctgt acacagtaat gatcagcggc gttcggattt 24120aacctaacct atcactgcaa ggtctgtggc tatatcgtgc tatgcgctcc acacctctga 24180gggtatgctg cttcccaaaa tgcctccctc acactcttca aagactaccc atacctcgcc 24240agccttgacg cgtggactct tacaggttac tactcaatgc tttttcctaa ccttagccaa 24300acctctgata aaaccagact taaaaaatca gccatcggga aatctttcgc acacttgcat 24360ttaacaaacc tttgctcaat tgcatagtga catgtgtatc agctaggaaa gaattaaaaa 24420caaaagcttg ctgcttaagg caaaaatttt taacacagca cagcagaaaa agccaaatac 24480cgggttcatc agtatttaaa caaagcactg gctcatacag tcttctcctc acagtgtttt 24540cttccttact ttcacagcaa acacacacag tatgctcaat tagcaaattt tgttgcattt 24600ctctaaacgg agtgattaac acataggctg actgctactg aaaacacctg acaaatcgct 24660tctcttgcac cctcaaaaaa gggtttcttt ttgagcctac cagaagttga aaacccgctt 24720gcgcccaggt ctaatataac agctaaaact gatcatttaa aaattacaaa tatttaccat 24780gagttgccac atcactctgc taaaattgtg ttttccgtat tattttccaa tagaagacat 24840ttaatagaca tctgaagaaa acaatacaat ataaaagcgt aagggtcttt gcaaacagat 24900cttctattcc ttctgcaaag taagaaagga gagagtttat tggcatttat ttgcagtgcc 24960atcgataaag acacgagaat acttaagaaa gcaaaaagtt ctagtgatcc acagacatct 25020ttggcttagc cttcctgacc aaagtcttct gtaaacttct ttaacttctc caggtcctgc 25080tcattaactg ttggctttgt gctagctagc gacctgagca tatcggcctg tgagaaagaa 25140agcaagaaaa catgcattca gaaaacgtac cgctgctaac agtattgctg tgaagaaaag 25200taagctttga aaagcctttt aaaacaagtt gactgtagga actcttattg aaacaaaact 25260tcgagtaagc ctgaacattt ctgcacgtgg accacttttt aacctcctga cgatagacaa 25320ttagtgagtt tttacaggac ttaagccaca atctgaggtt cagctttaaa acaattcatc 25380cattcaacaa gtgttatcta ccactgctta ctgcaacaaa ctgagcttcc catcttacag 25440attcgtattc caattcactt ttaaggacat caggttgaag tggaaaacca tcacacgttc 25500ccacatattc caatgcccac caacacagaa tacttcatca ttgatctcca gcaaagtttt 25560actgctcatg actgctaact tctgtttctt cagctcagtc agttttgtat atttacattt 25620ggctactaga aaatggagtt cagaaaaaaa aaccacagag gtatgaactc aaattcagca 25680gttaagaaac cttattaaaa aaaaacgtat ataaaaagtc ctggccaaag gcaaagcgag 25740gagctgctca acacctcacg ttactataaa agcacagggt taagttaaag tcagcatcat 25800gattttctag gctttctcat ctcatcgtac tacagacatc ctacttagaa agaattcaag 25860tctgatcttt ttaatgacaa gaactgattc tggactctga aataagtccc tgtgcaactg 25920tagcacatca gagtctacct tccattagaa gcactgaagg aattgtattt aattccagga 25980aagactgatg aaaaatccac ttagtttaca caggcagaag ttttaaggca ggcctgcact 26040tgcttgcatc ttttcatgcc tcctccatgt gcaaatatgc agatatttct ctcctcaaac 26100tagtgatggt tacatgtgca aagcagtgca ctctacttta gagggttttt gatccctatg 26160caacacacct tcctttcatt cattacagaa acgtttgcac acaggaatgg ccatcagcac 26220agatctgata tcgagtcctt ccttcagaca atgcaattac attcagaacc ttttgctgct 26280tgagggtaaa atatacgagt gctcaatgat ttgtaacctt ttaaacaatg tatttaaact 26340tcaatttctc tcaaatatga tgttttggtc tgtagacaga agcaaatatt ttaacatata 26400caaaaaattc cagctgaatg ttagcaagag ctggctgcat catctgtgat gaagtataat 26460ccaaactacc attgcatcca ccagcttttt acattgcatt ggttatgctt gcatttcttt 26520tgtgggcaaa atttacctac agcatgttat tcccagttta cactgaatat aatttcccac 26580ttctcgatgt caataataat gctacagagc aacaggaaag taacatatcg tggggcaggg 26640attctgaagg ttttaaatga ataaaagaaa aattaaagaa gggaggaaga ttcaggtgct 26700gtctatactg catgccacta gacaataata aatgcttatc agggatggag agctggctcg 26760ctgataagca tgttgtattg tcatgctgtg tgttgcgatt aaaatgtcat ccagtatgtc 26820caagcatgtc taaaaacaaa gggctcagcc aattgccttg catgctggct ctaaaatgtc 26880ttgagtattt tcagggttct gcaaagcaag aaacaccacc aaaaaataaa aaaataaaaa 26940caaataccca ccatggaaac tttaggctcc agtaatttat cccctggaac atccatccat 27000gtcatttctt cagcttcagg atcacctgga gagcaaggag tgaacaaatc taccatgata 27060tttggattcg tcactgatgg tccttttacc taataaatga atacataaat aaataaaata 27120aacaaactga agctgaacat ctttagagca aaagcatact cttaattttc tgtacatgcc 27180ccacccgttt ggagttgtgt agtgaagtgg aattgtgtaa aggtgctggc atcgttcact 27240ttgaaaacgc acagcagtag tcagatactt gaactcatac catgtcagaa ccaatgagcc 27300tttaaggtag gaatgcttgt agaaagctaa tgtgccaggt ctactgtttg gagaagacca 27360ctctcttctt agtcctcagt cactttggga gtccattcac cactggttaa catttctaaa 27420aaattctcag tagttattac tgactgaccc tcaagttggg ctgccatggg tgtcctttta 27480agcttccact cactgcacta aaaagttccg ggcacctttt ctgacacaat ctctaacagc 27540acttgataga agatggggcc atctagtgga ggaacagaaa ccatcccttc ttccagatac 27600atagacagaa cctgaaaagc tccatcagct gcctcttatc tttttgcaat gcatatctca 27660gacctgtagt tctaccatcc ttcctttgtc agtcactgaa gtatcacaca tccccatgaa 27720cacagaacac atgcaaaggc gaaaaaagaa ctgcttttaa cagcagagaa ctggatttgc 27780tgtttcaatc tgcttttaaa gcacagcgaa gaaaagcatg gattataata ctggaaactc 27840aacttggaca aaccgctatc aataggctgg aacaagcaat gggttacagt gagttacaga 27900aattgagcaa aacgctacaa acaggaggca ggggcagatg gcgattggga caagggggaa 27960tagtttaaac caacagaggg gagatgtagg tgagatgtta ggaggaaact tcttactcag 28020agggcagaga ggcgctggca cagctgccca gagaagctgt ggtgccccat ccctggaggc 28080gcccaaggcc aggttggatg gggccccagg cagcctcagc tggtgggggg cagccctcac 28140catggcatgg ggttggagct gggtgggctt tgaggtccct tccaaccccc aaccatccca 28200tgattctatt taactgggac aaactgctac tatggaaata gttaataaag caaaggtttt 28260tcttataaaa ataagaatct gcatccaatt aaagcacaaa caaaacaagt ggaatagact 28320tgcatcagaa cactcaaagc acggtaggct ttttttcctt tttggcaaaa gaggtaagaa 28380ttgcctttgg ctgctctgca aactgtggta actgagatta tttcattgtt ctgtggcagg 28440ctgaggcacg cctcagatgt ctgcaaattt caatgaaagg ctaaaatgtg acaacccatt 28500ggccagaaat gccatcattg tataaaaaca acaatggata aatacttcag gcatcactgc 28560ttaagggaag gaataaccca gaaaatccct gatatatcaa aatagccgct tattttttaa 28620gcaaatacag tttacaacag ctcaaaatac tgtttcaaaa tgttctttga ttttaaactg 28680ggaaaagttc atcaaaatac ctaccaaata ttcttcctca ccaccaaaat tacagactgc 28740tggcgtattt taacaagttg ataaggcttc ctcactgcaa gcactggaac tttaacagat 28800ctcttacatt ctgaaccata ttgtatttaa gcgttccttt cccttggtgt cttaagctga 28860atgtgttcct tacaattaca tggagaaaag tgcccacctt cagttcacac tgactctagc 28920tgttcagctg agggctctgg atgagttact ggtaaaaaac taagaaactg tcatcataac 28980tcatgagcaa caactgctgc caacacaagt tgcgtgtatg acacgcagag caataaaatg 29040aaagctctga aagcttccct ttccagagtc aaaagtccct gcagataaca agaatccacc 29100ttcacctgaa gtttgtgaat ttctgtgaaa acaaagtctg cagtacaaat gtaaacagat 29160tattttagtt tcgctctcta aaaccaaaac aacagcaaga aaaaactaga caagaaaaat 29220actatcatgt tatttataaa atgtaggcga aactccaaga taagcaaaaa aaaaaaagtc 29280ttatctatct atagttacac tctttttaga catcaactaa gtgtaaagta gttttcactc 29340tacagcagca tccataagat gttccttgct gccccagcaa tgacaacgac cttactcagc 29400cgtcttgcat cttaactact gtgacaagta acattagggg attcaatttt ttactggaat 29460cttaggataa tcttaatttt acagtttgaa ggacatcctg agcaaacagt tgtgcagttg 29520taattcctct gttcccacgt agataaggaa tacgtttatt tacacacatg cgctagaaaa 29580acaattacgt aatttgatat agaagaagag caccactgta agactccgat ttaagttgaa 29640ctccaaaccg aatgctttta acagcagtta tagacgtgaa gattgattag agcttggatt 29700acacaacatg aatacctaga gatgaggtgc atcaacttat ggcaggagta ctcctttggt 29760aggtaatgaa gaacagcata cacacatctg taagcacacg gtattacccc aaaccgaact 29820tggcttactt acaacaagtt ttcagatcaa gttaattctc agagttgaag caatatgaaa 29880aacgttttgt ttttacttac ttttttaaag tgagtagctg attgcacttt tctaacaggt 29940tgcatcagtg catcgcgtac aatgatgctt atatctgcac cagaatagcc atcggttctt 30000ttcccaagct cccgataatc tgcttctgtt aggagattgg gagtcgaccc gaggtgaagt 30060ttgaacatgg cagccctggc atggtcttca ggtaaaggaa

tataaattcg cttctcaaac 30120ctggtttcca aaagataaaa gcactggctc acgcaggtgc acgatggaaa gaagtttatg 30180caaatcagta tatactttgt ttgtaaatga aactgctttt ttcttatgta ttataaatgt 30240ttaaaaatat atatctcaga tattctgcag cctgttctca taagtaatac catggctatc 30300ataagctaac atctacaatt taacaacgac ttccttttta tgacagaaag tctcttcaga 30360ctgtagtttc tccaggttca ctccagagaa gtttgtttta aaagaaaata actgaaggaa 30420aaggagtctt ttagttttta agtacatctg aacagttttc atagaatctt agaatcgcta 30480aggttggaaa agacccacag gatcatccag tccaactatt cacccatcgc caacggttct 30540cactaaacca tgtccctcaa cacaacatcc aaacattcct tgaacacctc caggctcggt 30600gactccacca cctctctggg cagcccattc cagtgcctga tcactctttc agagaaatag 30660tagtggtttt tcacactcaa agaaagagct gcccgataac acgttcacac aaccagtttc 30720taaagtttgt aagtagagaa cgttgtagtt ggaaacgaat ttgaagtctt actctcaata 30780tagttgttgg taggaatggt tgatacttgc ggtgcttcct ttgaagcatc tgttctcaaa 30840gagaggacga cctcccatca gggaaatagg accgactcca agttctgtag aacactatta 30900acttcctata ggtaagtggg cccaagccat gaaaaattaa ttctgttact gccacgctct 30960acaagctcct ttaagttttt cggacaagaa tgagagatac tcgttcacac tgcaaagaat 31020gacttgaaat gttaagtacc acattcgcct cttattcctt gtatgaaact acacatgcac 31080aggatggaag cgacctctgg aggccacatg gtttaaactc cccagtcaaa gcacggtcga 31140gttgaattaa gtacatcgat aaaatgacac tgtcaccaaa aaggattgtt tctttagcct 31200acaaaaatta ccattataca ggttgtatca tcatcacaac ataatcacat ttgtcacgta 31260actgtgtttg tcctttgctg ctctgcaact gaaagatcca gctaatcaga tacagataca 31320aacgtcatcc cattagagaa aggcagttga aacgtacact gaaagatcac acaaactgtg 31380tgaccagtac agcaaaaaca atgcttctgc attacttaaa ttctgtgaaa ttactcaagc 31440tatccaaggg tttgctaaag ttgaaaacga tagctctgct gcctcttacc cttctgactt 31500gcttatgttg taccttgccc cccatgctca ccaggagacc agtcagcaac gaaacacaag 31560ttttttgctt agtcaagtgg aattagctga ctaagagatc agacagacta caagatatac 31620ataagagaga acaatccacc acttaagtga aggggatatt tgactcagtc cacctcatga 31680gacatgcctg caagaatcaa gtggatcact cactcaaata gcctcaggat gaaccctcac 31740aatagttgca aatttcttag cataaacatg aatacatcaa tcataggcca acataccttc 31800tcctgatagc agaatccaaa acccagggta tgtttgttgc tcctaagacc aatattcctt 31860cattatcaac accaacccct ataccaagga agaaatcatt tcaccattta gaaaataaac 31920agagactgcc tgataatgtt ttagaacatt tacaaaacgc aagggggtaa agctgcacat 31980cttttcacat gtaagcaatg cattttatgc gtagctgaac tcctttgatt ctgaaaacta 32040ttaaacttac cttgcatctg gactagaaat tccgttttaa tccgtctagc agcctcgctt 32100tcattttcac ttcttgaccc acatagtgaa tctatctcat caatgaagat aatagagggc 32160ttgttttctc tggcaagctg gaataggttt ttcactaatc ttaaaaaagg aaacagctgc 32220agttatctta ttgtacacac aagcaaaaac atgcaacttt ggattatgat acagtgactt 32280tgttaagaaa aagctaaaag taaaaaataa aatgaatccc acataagata ttaacaaagc 32340tactcaaaga tacaacatcc cttcagaact actaacacag cattaggctg agatgctgag 32400tgagatacca cagaataagg taactttagg cttcctagtc ttgttaacac atctcattgt 32460aacatgcaga gtggatatat caaaggcgct catcacttcc aacccatata tgcccatctt 32520ttatgtcttc aagattttgt ttgaaaacag aatgtagaaa aaaaaccttc acacagagga 32580agaaacaaca tgtattatct gcagggctac tgcaacagat gagccagaag gtgacaagaa 32640tcaaagtacc ccaacacttc agaccacttt gttgtacaat cacagctggg ttcagaaggg 32700cattgatcac cattgtgctg ctaatacctt tgtccaaact agttttaaaa acagtcttga 32760gtgctgaagc tgctgtagca caaaatacag tgcattatgg tacttttacc tgacactgca 32820ctgaagcaaa gaaacatcta aggtttgctt taacaagaca catgaacctt ccttccattt 32880aatttcttta gagtgtccta tctagctctg aaaaattaat ttcctcttga taatattttc 32940ctggaactct ggaaactcca acttacttct cactctctcc taaccacttt gagaccaggt 33000cagaggaaga tactgagaag aatgtggaat tgttcgcttc cgttgcaaca gcttttgcta 33060gatacgactt tcctgttcct ggaggtccaa atagaagaat ccctctccaa ggtgttctct 33120tccctgcaag aaagaaatca gctatcatca aaatgctgta tcaagagcaa gtctatcttt 33180ctgatgaagc ctccctaatg tactaagttt tctgtatgta cctaagaaac acctgtcaga 33240tcgatcattt acagctcagc tggagcctct gatatagcag cataatgctc ttctcagact 33300ccgcttacac tactcacttc aacagcagta tttagaatgg gaaataaatg ctgtaatact 33360gacctgtgaa caagtgtgga aatttaatgg gcaagataac tgcttcttta agagcttctt 33420tggcaccttc aaggccagca acatcactcc atttcacatt tggtcgctcc ataacaatgg 33480cacctataag aaaagattgg ataaatcact gatacgtatt tttccactgt ttgcttacca 33540tatatttgaa aaaagaaatc cacgtgtatg tttacattaa ataaaaacga gccatttcca 33600cacagatttc agcatcaaac agtgctactc aaatggatat tatttctaca gagatttggc 33660aatctttttt tctttaacca caataaacca tcaataagca gagagttgtt agaagttctg 33720cagtgtgcaa actaactctg caactgcgca gaaaacatac caatggcaga tacagaagag 33780tacacttcct aaaaagagat caacatgacg tacaccctga tgaagcaggc ccactacagt 33840aggatgcaca ggaaagcatg agcaaacacc ctgctgtgag cactcagtgt aaaaagaaag 33900cctggagtag agaccaacat caatctgtat tgcatccaaa ccagaagagg caaaaaagtg 33960tctcactaag ttgcagaaaa tgtgaacagt tcacacaaga cggattactg tggagagagt 34020aaatatgtgc actttttatt ttccccaata tgtcaccatt acaaaggaaa atcatggaat 34080ggtggagggt gatggaggcc cagcctgggg ccccaataca tgcagcaatg gacagtgagg 34140tcaccgacca agcggttgtg atgtcagcaa tggaaatgac tgtgtcctcg ctagccctca 34200ctgtacagat ttgggatctg gcagaggcca gcgtgtactt gtacctggac ttctactgag 34260catagctgcg agactcggag cactgagcga gttggttgag ttgtgctgtg gggctgctgg 34320cagcagttct tggtgcccac cccacagtac caccaacgtt tcccccagcc ctgcctgtct 34380caggcagctg gggccacaca gggtgcactt gtagcagcag aggtgagtgg tgcaggacat 34440ggcctctgcg gcggctggtg gggaagtggg agggtttgct gctgagggac caggacatca 34500cagctgcctg cccatgggac gagtgaccat ggcctctctc tctctttgca gttcgtaaca 34560ccttctgcct gctgcagtac ctgtgagggg agcagcttcc cgacctcagc tctcccagcc 34620caccgcacag cccggggcca tggacgtgcc atctaactgg acctgcccca tctgcgggca 34680aattcgggag gatgtcacct atgtgacccc ctgcaaacac cagctttgct acggctgtgc 34740catctggtgg gcaaacaaga agccgagttg tgccgtatgc gggcaccaaa tcaccaccat 34800ccgatactcg gtgaggtcgg acgacgacta cctcgagtgt gctgtcccgc agcccgcagc 34860acactctgat gatagcctgc aggatgagca ggggcctgca gagccggtgc tcatcccacc 34920tgagcacaac ttccctgccg aggtctgggc tgccttcttc aaagaacatc agggagacct 34980cgagcccctg ctccactggc tgcaggagga gatccaggag gcgtccagca gtgactggtg 35040ggaggtggaa gtgggacagt ggaccactgt caacttcctc tgcgagcacg gcctggacga 35100ggaggccttg atgcgggagc tgcagccgat cactaacggc gatgtgctgc cctttgtaag 35160gcagctcatc agcaccgcta cagccctgta cggcccagcg atccgccgcc agctcgacca 35220ccaggaaggc cgtgctgcag gacagcggga ggacagcccc gcagccagcc ccagcaccac 35280cacctcccat caggagcctc ctgcctcggg cctgggccac tccaccagcc ccgcagggcc 35340cagcaccgag gagctgcccg gcagctctac tgggggaccc gggcacccca gcaccaccac 35400cgcgccctca gcggaggagt cgcaggagga gccatggcag gcggtggcag cgggcccctc 35460cgcccagggc agggaccgct cgtgtggggg gccccggcgc cccccgaaga ggaaggcccg 35520cagcagcccc caggcctcgc ccccacctcc caaaaggcgg ccccggcggc ggcgctaggc 35580tggcaccgca ctgccgtcag agcacagcgc cagcgggctg ggaggccaac atctacctct 35640cggcctgctg cttgctggca gaataaacat cagttaaaac aaagaagaaa atgtctctgt 35700gttattgaca agactcttgc tgttgctgtc cctacccatg ctgctttctc tctcttccgg 35760tcctagagga gagaaatgca actttatttc caccatcata attcagcatt catgacagta 35820ctaacaaagc acacataggc tccaaaaagc cgaagatgga cccctcatgt tgctctaatc 35880ataatccaac caccaggact tggctaaatt cctctcctat tgccaagctc tgggccacag 35940attacttcgt ttgattttag ctgctgagct gtggtgtccc cctcccttca gacttcccgt 36000tagtcagtct gaagataaaa actctgttac cagatgactt ttagatggga cagctcacat 36060ctgagctagt gacccagctg cacattttga aaccctactc aagacaaatc caaaaggcaa 36120gagaaatctt cccaaatgaa ttaatgccaa ctaccccaat gcttatcttt ctgtactcaa 36180gcacggtgaa ctgttcagtt gccatttttc tctacaaagg gctttctatt agttcacaac 36240cagtttctgc tagctatttt cttgtcactt tccccttgtg ccttcagagc tctgtgaatt 36300ggttgatggc cattttctac aatggaaagt gtaccgctac tcgtggctaa caaataaagc 36360aagtgacatt tgttcacttt ttgtccatct ccttagagat ttttactttt cctgcacgcc 36420tttctcatca gatagaaagg aatatttttt gcttgcaatc tatatacagg aatccagcca 36480ctcactttta atgccctcaa tacttttgct aggttgatta caactcagtt tttcctgtaa 36540ccaggctcca tcactaaatt aattagtagg acaagtagga acatgagatt agttccaagc 36600tatcagttat gtggacctgg catactgtgg taatttaaat tagcacactg taagacatta 36660cccataccag gaaacaaatg gaacaggaca tcgatcatgg cttcctcatt ttgtaggtgt 36720aaaagaacag ctggaagact aagccaacag agcgcaaaag gtctttaaat atcaagctaa 36780gccacttctt ttctatgtaa aaaactactg ctagctgcta tatattgcat cactggatgt 36840gtacagcacg ttatttcaaa aacacaaaca attatgttac tcaactgagt aacacccctt 36900atcactgcaa cacgaggaaa tcccgcctgt tgctatgaac aaacaagaat ccatcttccc 36960gccttatcaa cttgagttca agccttcctg tgaaaatggt cctgcttata ctacgtactt 37020ggatgacatc tgttacttgg atgacatcta ttgcctctag gcaataatat gtcaatgcac 37080ataagagtaa aactagcaca gtctaacaaa atagctatct gggatcttgc aactactccc 37140tttggaaaat gttttcttga taaatgatcc aatttcaaca tatgcaccac tgaatttcat 37200ggcatgcaaa cccatactgt cataaagact gtacttctgg atgtaaagag tatatactag 37260ttgagccacc taaagacaac aagttaactg gcaaaacaaa caaacaaaca aaccccccaa 37320acaactagaa attcacttga ccaaagtcac ctctatttaa ataaatggag gcttcaaagt 37380taccttgaag ctgattctgt agtttctttt tctcaggatc ctctgactct ccttccccat 37440cactgtcatt cctgatttgg aaacaagaaa taaaacgttg aaatacactg agaaactgct 37500gtcctaggtc acaaatcaga aagcaggaag tagaaaaaac atcacttcga ggaatgaaaa 37560accttatgat tttagatttt ttcagctctc tacaagttta catccttgta gtcttgtttt 37620tctacactat attctaaccc ccccctctca ctgcaaccat ttcaacttct gtacagaccc 37680gagcccttcc tcttaacaca cttctacatg tgttgactca gcctctagga aacaaaagca 37740tcgtggaagc agcaaaatgg cttcactgta gatgctggca cttactcctt gtccagaatt 37800gcaactggtc ttggtcaatt ccatttagta ctacgaaact ctctagtctt gtcagaataa 37860aggaaactgg aagttaaaag tagaaaaaag tagacgagct aggggacaaa tggaatggag 37920acgtgtagcc tcatgtttcc ttctactata aaccagcaga acacagtaca gctcaaaaaa 37980ataaaaccca tgaaatgagc agacaatgaa agaagctgaa aatcagggga ggtttcaaga 38040gacaactgag cagttctagc tgttcaagac taccaaaaag ggcaacctca cccagagaca 38100ccattgtgaa acctttcttc taccctagca aatacaaaag aggctctgct tgttcaggtg 38160ggctgattca gctctcagat gtgcaaagtg aaaaacagat gtaataaaag gagagcggtg 38220cataggcagg agcaaggcaa tagagcgatt cagaccatca gaacatcagc ctatgacaga 38280accttggaac ccctcatcaa atgtgagaca gataaaactc agaccacagt aatcatccaa 38340accaaaccaa ccaaatctgg acttattttc tagtcattaa gtattttcat gcagaagaat 38400tgtgttacta ggctcactgt catcgaaaca aaaagtatta gtgtaaaaca gctttcattc 38460ttcagtgaat gtcctacaga agcattgaaa gatgtagcaa acaagcacaa aaaagcccat 38520aatattaact cacattattt ttccttttta agcccactgt ccttcagcat tagtagttac 38580ctgaagcgaa gcacttcaaa aacactactt aaaatgatct ctgttgagat ctaagttgaa 38640tcttagaata agcggagttc aggaagtatt ttgctttacc ttctcccaaa acataccctt 38700ttccatcggc aggaccagac tctttaactg gctttggtgc agttttttct ctctttttca 38760gatattcttt cagtttttct gctctgtcca agtattccgc acatttcact ctaatgctct 38820gttttgcttt atcaccctgt gtttcatcta agagtgtgaa aagaaacaat gcgttgttaa 38880caacaaaaca cacgtgcatc attcagaaaa catctttatg tgttatcaag atacctctct 38940cagggctcac cacgcatcca aatgtttcat ttacttattt tttcccctat gccatggaaa 39000gaagtgacag gaaagaagtt aacgcctaca aatcaatggt aagtaatcac tttcaaatca 39060aatacacacc tgaacgttgc tttgccttaa aaacttgcct gaacacgagt aaggacagtg 39120gcactggaag ctttttctgt cagtctctca aactgctata tagtgtctta actacttttc 39180taaactaagc cattgagagg ctgacttctt gtttttagag accttttttt aatctaagac 39240cactttattt ttccccggcc tgctaatttt gaaagttgtg cacatcaaag gaagaaaaaa 39300gtcacaaaac atctgaaaaa atgaggagtg gtccaacagc cacagttctg ttagtcgcta 39360ctgcagtatt ccagatcagc aatcaagctt gaaaatatta agttcatgcg ctacgttccc 39420aaaagtccat cagtatggtt aaaagcatag ggaagtaagt ggcatgagtt aatgagcaca 39480aaacaacctg tggatactac taagagttct tacaagaagg gagcaggcat gcaatatgca 39540acttttgtcc ttgctataat ataacacctc agccaaacta cagagagcaa gtgtcaactg 39600acaacaacag tcagaagtta aacgttgatg tcgacagagg agactactcc gggcaatata 39660aaacttgact tcatcacccc atgcattaca cttacattta acaacgtgga ttaaatactg 39720cacagcatgc tggtacaaac ggaaggcttc ttcatagttt cctgctttat cttcttgtgc 39780tgccttacta gcgaggtcta tcgctttctg taacataggt aaataattca aatgagtgtt 39840gtgtgagtgc tttgtgcgat caaagaggtt tttaagctgc tgctctgacc gcttcttggt 39900ggccagcttt tctgctcctt gatgtttacc caaaagagct gctgttattg aagacttgct 39960gtcagttgtc ttcatcaaat cccatcggca tcagtgttga tactggaagt acacgattac 40020aaagcaatga aagcagcacc ctttcccttc tgacccagtg ccaggagttg gtttcaaaga 40080ctcattattt ggtaagcttc tcatgaaggc tttaggtact tgacgtacag aagtgagaaa 40140ttctaaccat ctcttcagtg tgcatatggg ggggagctca gtggacagga aacataccta 40200aattatcaca gaagttctat caaggacaat ttagagatgg atttttattt gtttgttgag 40260ataatttcaa atacatctgg tcgtaatcta agacactaca tcggcctgta gatatattga 40320tattactgtt attcctttga tcccgagtgc tttttattac atttcagatt acattacaga 40380tttttattac atccttggaa catccgtact gcttcaggac aattaagaat gacaattcca 40440atgactaagg cacgtatgct taaaaaagcc agagttgact aacgctacct cgaacttcta 40500cagccctgtc tgcatatttc caccttctgc cagtttattt cccaaaggca gggacagcgt 40560gctcgtgatg actgtgctaa catcagggag caaggtgaag atattcaacc tcatcacagg 40620gttttcacta cacactgctg tgcacatact ctcaacagta accagacgct ctgatgcatc 40680tcagtcaaaa ccgagcagat aaactgcagc catcagagaa ggaggaacaa catttctcct 40740tctattgttt tgtcttgcct ttttggaagt agagatcacc tcattggatc catctgaaat 40800caagagtaat ttatttcaaa acaatcacct gacaagtaag actatggatc ctttgtgaca 40860agtgttgaaa acagagcaac catctgtttc tttgaaacag aacttggtct ttcctcactg 40920ctgacctcgt gctgccctct acaaattcat tgtagagggc aaaccattca aattcagcac 40980aacaaaaata aattccaagc aataatttct gttactttag tgatttaatt accacaggaa 41040cagtccaatg attcctggat gcagaacaac aaaaacaggg ctatgacaaa aatgacaata 41100tatccaaaca acaaataaga gttggacttg atgatccctg tgtatcactt ccaacccagg 41160acattctatg atgctatggc tctgtgttct aaatggcaaa gaccgcctct gttcaatggt 41220aactctctta acagggcatc ttagagccct gctcctctga aatacaaaaa caaaggtcta 41280catcctgtgc tgactgtttt tggtattttt tcaaataaaa acccagaaaa ccatcacttc 41340ggttttagac tctcagctct ggtactttat tacattagga aggctcttag cctgctactg 41400caatgaaaaa caccagtaac aaacaggaaa taatttatga aagttgtatg aaataaggca 41460tagctgtaac cataaatgag gcacaacctg tatctatggg gctatagttt gagagctgga 41520tgaacaccac cctcagaacg aacatcggct ttgctcttct gcttactctg ggccctctga 41580tttcacagaa gggcgcaggt tggaagggac cgtaaagccc attcagttcc aatccccctt 41640gcatggtcag ggctacaccc caccagctca gtccgcccag ggccccatcc aacctggcct 41700tgagcacctt caaggatggg gcaccacagc ttctctgggc agctgtgcca gggcctcgca 41760accctctctg agtaaggagt ttcttcctaa catctaactt taatctcccc tcttttggtt 41820taaaaccata cccctttgcc ctacctctat cagaccatgt aaaaagtcag actccctcct 41880gtttataagc tcccatcaag tactggaagg ctgcagcaag atctctccca gcttggtcac 41940tataagcact acatagcctt aagcttacag gcatggacat ggtttaaata ggtttaaaac 42000tactttttgc acagattatt cctggatcta ttttgaaccg gcaacacaag cagttcactc 42060ccacaaccga aggctaaaat aaaataaaat aaaataaata ataattaaaa aaaaaaaaaa 42120aaggaataga gagcagacaa gcatttccaa gagtcgtact ctcagcagaa acccagtcca 42180aactacgcct ccagctcaca gcaggccgca gtcttgcctc agaggccaac gggtcttctg 42240gtcccagccg ggcaggtgac tacccggggt cctccggcgc ctccgagccc ccacccaggc 42300ctgctcgacg ccccaccgct ggtgtcagcg cttctgcccc caggcccagc ctggcgcccc 42360accccgccga gcccgccctc ccacccgccg gctgcagcgc accggggttc aacaggaccc 42420gctctacctg caagttgccc gacatggcgg ggagccggga aggggaagga cacgagacga 42480cactggctac ggccgaccgg agctgccctt cccgccaccg ccgcccaccg aaccgaaaag 42540ccggccttcg ctagccgctt ccgcacctca gcgccggccg gcccgcttcc gcttccgggc 42600agcgccccgt acgcgtcact tgacgtcagc acgccgcgcc tcgccccgcc ctatccgagg 42660ggctgagcgc atgcgggccg ggcgccggaa gcggaagttc gtgggttggc gcgcagcagt 42720ggtgctgagg gaatgggggt ggtgttaggt ccagcactga cgtaggggat agggctgaga 42780tctgatcatg acctactgtg gggagcctgc tgtagcagag gttgggctgg atgctctcca 42840gatgtccctt ccagtccctg cgattctatg atcatttctg taaaatgtta aatagtcact 42900tatagggttt tgaataaatc acgttttttc ctcatgcctc acgtttggga cacaaagaca 42960tttttttctt acatctcttc tttctcgtac catttgcttg ctttcagcgg cactgtcttt 43020tgcataatct gagtgcagaa tgctttttat tcacagaacc agctcttaat aattcctgac 43080agtcataagc agtcaggcgt tagtcacctg cagctcagta atgaaactca actaacaggt 43140ctgcagagta agagcaatga cgtgactcag aaagcacagc acattgtaaa caactcttgt 43200aaacttgcta tatgggtttc agactaatga acttctgcta agtcggtgca acagttgtgt 43260taaattactg tcatatcctt ccctatgtta ttgtaatact gttgaggaaa tgcttcctta 43320gattcacaat cctcgttttt ctacctgcct ccaactaagc ccagtacagt ctgctctggg 43380atgaaggtaa aaggcacaag cacagtcagc cctatatcta ggaaggttga tgtaatttct 43440tcctaaagtc ctctgcttgg cagcttgttt tgcttaatgt cttcatatgt gcacaccagg 43500caggatgctg aaggctcgtt gtttggggat gatcagtaac agctgttctt ctattgcaaa 43560tgtgaaaggg tacaatgtag caaaaattcc tggatgtaat caggctctgg gaaatgagaa 43620ggcaaaggaa atgttggagg taagagcagc gttcaggaac cagaatgata tgggttggaa 43680gggatcttaa agatcataga atcatagaat cgctaaggtt ggaaaagacc cacaggatca 43740tccagtccaa ccattcaccc atcaccaatg gttctcacta aaccatgtcc ctcaacacaa 43800catccaaatg ttctttgaac acctccaggg tcggtgattc caccacctct ctgggcagcc 43860cattccagtg cctgaccacc ctttcagaga agtagtattt cctaaagtcc agcctgaacc 43920ttccctggcg cagcttgaag ccattccctc tagtcctacc actagtcaca cgagagacga 43980ggccgacccc cagctcacta caacctccct tcaggtagtt atagagagca ataaggtctc 44040ccctgagcct cctcttctct agactgaaca atcccagctc cttcagccgc tcctcataag 44100gtctgtgctt cagacccttc tccaactttg ttgccctcct ctggacacgc accaggctct 44160cgatgtcttt cttacagtga ggggcctaaa actggacaca gtacttgagg tgcagcctca 44220ccagtgctgc gtagaggggg agtcatcttg ttccaaccct gttttcctgt aggtagtatt 44280tctggctgtg ccatctgtac ctatggtttt caaatctgta atgctacacc tagcttttag 44340acctaggtct aaaacagtac acaagtcaca ggcatgttag taatgcctct ccagtcacac 44400tttgcagtct tccgaaactc cacatataga catgtttcta tgattgtgaa tgagattaaa 44460aaaaaaataa attaataaat cagaaaaggc acgtgtatat ttacagataa caggctaaat 44520attatacttc ttaattaagc tttactatac agtattcctg ttatgtgact ttgcagctag 44580ttttgcctaa ggaaatactg gctgaatgct gagtaataac atcacgacag actcctgagg 44640agctaatgaa gtattacacc aagagtgtag cttcagtttg agagacgtgt atggtcacat 44700tttggaatgc ttcccattgc tgagttgctg tgttacaata ttctcaaaat ccgtgtcagt 44760tattgtgttc aactgagtgt aatgacaata aaatatatta atgacgttaa atgaagatat 44820catagaatca tagaacatcc caagttggaa gagacccaca gggatcacca tgtccagctc 44880ctggctccac acagcaccac ccaaaattca aagttgatgt ctgagagcgc tgtccaaatg 44940ctccttgaac tctggcagct tggggctgcc ctgggcagcc tgttccatac ccaccaccct 45000cttgttccct cgggctctgt cgcagtcaca cagagcagag ctcagcgctg cccctccgct 45060ccctgcgagg agctgcagcc gccaccaggc ctcccctcag ctcctctgct ctgggctgaa 45120cagaccaagg gctctcagct gttcctcata cacgttgccc

tccagatcct tccccatctt 45180tgtggtcctc ccttggacag tctctaatag tcttatgtcc ttatattgtg gcacccaaac 45240ctgcaccctg tgctggaggt gcaactgcac agcacagagt agagaggaca accctttcct 45300gcactcgatg gcagtgctgg gcctgatgta ccccagggta tagttggccc tttgggatgc 45360tagggcacaa cgctcagtca cattcaactg tctgtcaaca agtacctatt ggcctgcatg 45420aggcctgtct gctaattggg actctattaa atcacatcac tgtgacacta ggtggcacag 45480gcacacatga tctccatgtt ccttaaggct gagtgaatca tggagaatgc ttcctgctat 45540cagtttttgg catggaaaga gaggagccaa accaccggtt ggttcaatgc cttgtgccag 45600gaataggtga atgcatcaat acaataagtc acgtctacag cacagccagg cctcatgtca 45660gcaatactgc tccactgtga tagctgaaag tgactataaa tgactaacgt tagtgtggga 45720ctttggtgtt agatgacgtg agagccatgc agtgaaagag aattagtgtg gcagagtatc 45780taacagtgca ggtagataag gcaggaagga taagtgtaag gaaagataag gagaaaggca 45840ggaaagtaaa acctctgttt ttctctagtt ttctacctgg tgaaatgatg aagaaagatc 45900agtttgacat aggttaacaa aaactgtcag taagaaaggt aggagttaag atgcatgttg 45960tccaaatccc actacattac tttgaccctc ttcagcatat gcacaatgag atcacttgcc 46020caagacagga cctccagtgg gcatgaaatc tgaaaatcaa ttatttgcta tttgtgttgc 46080ttatcatttc cagatgaaat tctacacgag ataattagag tgatgtcctt gaagatcaac 46140ctttttgtct aattaaggta tttgctatag cttccagatg tattgcttat ctatgataaa 46200tatccttcct aactacaagg cttctataat aagagtaacg tcctctatag taaccagtag 46260aaagtaggtg gaagctgggt gttcttagac aacctgtgcc catacatgga caaagtgagg 46320aggaggacac ctccctaaat gaccaccaga gaccactgaa gacccacatg caagcacaga 46380agattcagat gtgttggtgt aaccttgtaa acgcagtaat ctcgtgaata tgtgatagat 46440aggtgtgcct tatgtattag ataggcgagt attgagaact tttggtttat ggatgtggat 46500agtgctgtta tccatcttgc accctgagca taaataaagc aatatctctt ctatagtgcc 46560ttgtcttttc attgtatttc aggagacttt gaaactgaca acaggcatgc agcttgggag 46620tgctcacagt cagtctggcc acagtgcctt caagcctccc ctgcactggg atgtggtgtg 46680acaaaaagca caaacactgc ttttgtagaa gacccagacc acaggctgca ctagggaacg 46740tgtctgcctg gagcacagtg ccctggggag tgctgctggt acagtagtcc tggatgagtg 46800gcttccttct gtaacctttt aattgcacta gaagtacacc agcatggcag agaagggctg 46860ggtcctaaga gcccttcttt caaattcact cagaactcca gatgtttagg cagggtgttg 46920tagctgtaaa gtccaggaag aaaaggttta aagctgtact cggcaccaga aagactggag 46980ccaaaataaa gccacattgc acccatggca ctataggcaa agggtagcct tggggcaaga 47040cttgatgtac tagaagttga ggagtcctca gactctgtgt caaggggatg tgccacaact 47100ctactgtgcc cctacctgaa gcctgaatca gtacaaatgt ctcacgcatg ggttaggcat 47160ccttctctca aagctcttgg tctttgcaca ctttcttctg cagctgcagc agcagccaaa 47220ggaaaattag gtcttgcttt gaaagccagc cccttccagc catgactggt cccttctcac 47280tccacatctg tggatgatgc tcccacagca ggtgggagag acagaggctt tcttgaagaa 47340acccagcccc tctaggggaa cactgtaaag tcacagggga ggagacgtgg ctttgagaca 47400gtgatatact ccatgcccct ggcgttcttc ccctgagtgc cactggtgct gctcagtggt 47460cacatgccac caaagtctgc attcatcttt aaatgctgct gagaattcaa cctttgataa 47520atcatctgct ttgacaaaat cgacatttaa aaattaatat ttcctcttcc atcccctact 47580tttacaggct ggctcaagaa aatgggaagc ttaatgtaga cttgggtctt actaaaccat 47640ttcactggga aagacattca cagtctgtgg cagatggtag cagtatattt tctctcatag 47700tacaggaatg ggtctggtag tacctctttg gaaaggaaaa tgtaaactca tacgttttga 47760gccaaattcc atcagatttc ttagttttgt tagttttcac tccactcctg ctggaaactg 47820aaaatatgga aatgcttgga aatttactgt gatttgggtt caggtgtgtg tatgcaggaa 47880atgtgttacc ttccagagta agtcagttta ttctagaaat gggatgactc cacttttata 47940cacttgtaat tcacagtgag attaatccag ccaattggga aaacagcctt tcttaaattg 48000tgaaaaacat gctccacttc tatgtatttt ttaatatact tcagcattgt gaatttgaag 48060tttttcttct actgttacat gcattccaac agaatttgtc aggaacaaaa atgaaatctg 48120aaataatatt tttcttagct ttgcatgtgt tatcctcaag ggtaatcact gtcctaaaca 48180acatacttat ggctgtttct gagcctttct tcttcatgaa ctcatcagaa agggacactc 48240atattggcag tctgtataga gagccaagga caaatatttc gcctacgtct tctctgcgta 48300gcattttata tattaggtct tgctagtgaa ttatgactga atggaataca gtcccttcag 48360tgatgacttc attcatgatt gaataaatgt agcttcaggg ctgtatggtt gacttacatc 48420atccaatttt gccatctgca acagccaaca cctctaccca tatatgaatt cagcgaggga 48480ttttgtacta tgtgttgctg ggatgtagca gcatttctct ttgaaatgtc tttacagatg 48540caatgcctag caggcttaac agccctacct gcttcagaga cactgctgta aaaagaaaaa 48600gagaagcttc ccagccagta tttcatcaag ttaaaaaaaa tctaaaagtt tatactgtac 48660catttggatt gctgcatgtt gacatcattt aggattctga aaacctaaag aagctttgga 48720gcaactccta agtgtatggt agatgctctc attatgtaag agtgacaaat cactaccagt 48780cttccaaaaa tgcatgctga aatcaaaaaa gaaataatgg atctcacaaa actggatctg 48840cagatcaggt tctacagcct ctggtatgca agggttaaag tagagtgatt gttgtagctt 48900gtgtctcaca gtcagacata aatctgtaag caggtccagg ttttgtaaat tgttgcttat 48960caccacatga gcaataagta atctgaacac ccaatgtaac agatttctag gagttagggc 49020tgaaagcatc atgaagttta ttcttttcta cagcaaagca ggctctgtgt acctgtctag 49080ccacattgtc tctgacaaaa tttatcatca attctcatct ccatcaactt ttaagaatta 49140cagaattgaa gggagggatt gttgaaaggg atctctggag atcatctagt cttaccccat 49200gatgaagcag gttccttaca ataggtggca taggaaagtg tgagcaaaca ccctgctgtg 49260agcactcagt gtaaaaagaa agcctggagt agagaccaac atcaatctgt attgcatcca 49320aaccagaaga ggcaaaaaaa gtgtctcact aagcttcaga aagtgtaaac aattcacaga 49380agatggatta ttgtggagag agtaaatgtg tgcaattttt attttcccca atatgtcacc 49440attacaaagg aaaatcatgg aatggtggag ggtgatggag gcctagcctg gggccccaat 49500acatgtagca gtggacagtg aggtcaccga ccaagcggtt gtgatgtcag caatggaaat 49560gactgtgacc tcgctagccc tcactgtaca gatttgggat ctggcagagg ccagcgtgca 49620cttgtgcctg gactcccgtt gagcatagct gcgagacttg gagcagtgag cgagttggtt 49680gagttgtgct gtggggctgc tggcagcagt tcttggtgcc caccccacag taccaccagc 49740gtttccccca gccctgcctg cctcaggcag ctggggccac acagggtgca cttgtagcag 49800cagaggtgag tggtacagtg gggaagtggt ggggaagtgg gagggtttgc tgctgaggga 49860ccaggacatc tggacagctg cctgcccatg ggacagcgag tgaccatggc ctctctctct 49920ctttgcagtt cgtaacacct tctgcctgct gcagcacctg tgaggggagc agtttcctga 49980cctcagctct cccagcccac tgcacagccc ggggccatgg acgtgccgtc caactggacc 50040tgccccatct gcgggcaaag tcgggaggat gtcacctatg tgaccccctg ccaacaccag 50100ctttgctatg gctgtgccat ctggtgggca gagaagaagc cgagttgtgc catatgtggg 50160caccaaatca ccactatccg atactcggtg aggtcggatg acgattacct cgagtgtgct 50220gtcccgcagc ccgcagcacg ctcagatcac ggcctgcagg acgagcaggg gcctgcagag 50280ccggtgctca tcccacctga gcacaacttc cccgccgagg tctgggctgc attttttgat 50340ggacatcccg aagacctcga gcccctgctc cactggctgc aggatgagat ccagcagttg 50400accagaaatg ggtggtgggc agtgtgtgtt ggacagtgga ctgttgtagg cctcctttgt 50460attttcggac tggacgagga ggccttggtg caggagctgc agccattctc tgatgctgac 50520ttggtgccct ttgtaaggcg gctcatcagc accgctgcag ccctgtacgg cccagtgatc 50580cgccgccagc tcgaccagca ggaaggctgt gctgcaggac agcgggagga cagccccgca 50640gccagcccca gcaccaccac ctcccatcgg gagcctcctg ccttgcgccc aggccgctcc 50700accagtcccg cagggcccag caccgaggag ctgcccggca gctctactgg gggagctggg 50760caccccagca ccaccaccgc gccctcagtg gaggagccgc aggaggagcc atggcaggcg 50820gtggcagcgg gcccctccac ccagggcagg gatcgctcgt gtggggggcc ccggcgcccc 50880ccgaagagga aggcccacag cagcccccag gcctcacccc cgccccccaa aaggcggccc 50940cgacggcggc gctaggccgg caccgcactg ccgtcagagc acggctccag tgggctggga 51000ggccaacatc tacctctcgg cctgctgctt gcagataaaa tgtggggatt caagaaagaa 51060tatttagagc acaagctgca gaacaagata aacagcatgg gaaaggaatg ctgaggacag 51120aggatgcctc caagagagaa gaaagtcaag tgagctgcat gatcgctgcc taacaatcct 51180aattggaaga agagtatgtg gctaggaatg actcataact ctgattggag aagcgcctgc 51240atgcgtggtt aaggagtaga acaagagcaa gggtgaccct gtgggatgtt ttgttgacat 51300gtaaaggggg tgggaaagat accagagaaa acttggcagt gtatttaagg gatattagaa 51360tatgcaataa atgatttgga ttgctcatac atctgagtcc gtgccttgga tgctgcaaga 51420aaataaacag aaattcaaaa aaaaaaaaaa aaaaggataa gaaaatgtct ctgtgttatt 51480gacaaggctg tgggcgttgc tgtcctttcc catgctgctt tctccctctt tttctcctgg 51540aggtgagcac agacatgcag ctttatttcc atgaccataa attggctttc atgacagcac 51600taaaaaaaca cacgagggct ccaacaaaca gagaaaggaa cttatgttac tctaataata 51660atccaataat cagggcttca ctaatttcct ctcatactgc cagctccagg ccacagataa 51720ttaagttttg tttgatttca gtgactgagc tgtgatgtca ccctctctgt agacttccta 51780ttagtctgat gttaaaaaca ccaaaaatat gtgctgtaat ccaaagagaa attatgggtc 51840ccattaaatt ggtactttgg gttctacagt ctctgttatg caagagttca agctaaatga 51900ttgctgtagc ttgtgcacga gttttgaaaa gataccaatc tgtgaacaga cccagatttt 51960ctttctggaa ttctcctccc ctgtgcaaag gaaagcacat tgttttttgc tctcatcaga 52020gagtactctg aaatgaacat ttttgagtta gacagtgagg agcagaaaag aaattctatt 52080cacataggtg cttttaaaag cattaccaga ttcttctaga caaatgacag aggaataact 52140tttgccattc cattacacaa tagaataact gaactgcaaa acaaagagtc acgctacagg 52200agtaagtttt gaaactgact tgcttacctc tgatgcttcc agctgacttt ctccattctc 52260acagtagatt caaagttctt ttttttttaa ctgtgtgact gtagagagta gtgttcacaa 52320cttaactgca tgctgtgcag tctgaattag agctgggggt aggtgataac acacctcctt 52380caactgtttt gttttcctga actgtggttt gtctcattat tttcttctaa atgctatttt 52440aagcagtaag agtttaaaca tgccttctgc ctgccttaga actgcagaag accttaaatg 52500cagaactctt actgttcttg aattcatggg aaggtctgag gaaatggggc catccaagat 52560gtcctccaaa caatacgttc cctcattcca ttatgtgtaa ggtacagtgg tgttgtacca 52620gggggtgagc actgcagtgg taagtgctgt tggacctgtc gtgcaagaat agaaagaagt 52680cccacaacag ccaaagtcca gtggctggac caggagtagc caactatgtg gctgctgtga 52740tttgatccac accagatttc caggttagca ttttcctctt agacccatcc ttattaatcc 52800ctaagccttt taattagttc ttgtatggaa agtagcagaa actgtatagg aagtcattta 52860tctttctctt catcctagcc actcttacca gagtaatttt catcttaagc aggaagctct 52920tcaagccagg ctattattcc atcataaact gtctataatt cttctacacg tatgacattt 52980tgtctacatc ttccaatatc tgtctcacta acaagcctgt ttctgttttt tatccacaac 53040ccatcgaatt tggtagccat ctttgcagtg ggctttggat cttgacccaa gaaaggaaaa 53100cggaagggta tttgcactgt cacaagttcc tatagaccta attgcagctt tccaagtcac 53160ttatgcctgt tatgtaaatg ttaacgctat tgtggagttt attaactcgc tggattatgc 53220atgaagtatc ctctggagtt tccccatcaa gcttaatggg accattagat ctcagaagaa 53280tgacgaaagc tatttctcag tagcttacat attacctggg tagatgtaat gggaaagaga 53340aaaagaagca ttctgttatc aattcctagc actttctttt gttaaatata ggctattttt 53400tttatcattc acaatttttc ctacttttcc tttttttatg gcctagtatg ttctgtgctt 53460tgttacacaa atctagggat cctgggttag tggtgatatg agctgaatca gctgctgaat 53520gtaggaatag ctcacttgct ttcatgggtg ctaatcagtt tacattagct gaggttcagg 53580gccattgttt gttaagattt acatctggat gtcaagatgg gtttgcaggt ataactttta 53640taagtgactg gtgagacagc gacactgtag ggtgttttac ttcgagtaat gcagaagaat 53700gtacactgat tttgttgcct ttagcagatc tgccaaatac caactgaaga agcaagaatt 53760aacatgtttg ttcctcgtct tagttgcatt cagggacaag aaaagctcca tcctctcctg 53820aaaatacaca gctggagaaa attcagacca tggaggcaga cccatttcca gtgtctattt 53880cagcaaatat tgactctaag cttttattgt cctttaatat gcatatattc atgctgtgaa 53940tctatgctga agaactctgg gaaggtgtgt gccctcaccc acattaatca cccagacact 54000tcataactac ctggattaca ggagaaagtg actcatctac tgatgacgct ggataaaagc 54060aagaggggaa agaaatcccc agttctcaca cctccctcct ctgcacatag taaggaggca 54120ctcaagggca taatgcaaac ccagagctgg aagggaggct gtgtgtcagg gcccaggcct 54180gctgctgtgg gcagcaaagg ccatgtaatc gctggacatg tcagttccta ctgctccgat 54240ctgaaaccag ttcagagtta gagggagagg tctgcttggt ctctctgcta ctcatgggaa 54300aagcacttct gccaatgtga tctactctct cttcaaagtt tccacattgc ttacgtgagc 54360aatcctatcc ccatgcaggc tttcttttgg taggtgagcc cctgataatt cgaaaatcac 54420acctacactc agctaggagc taaccagatc tataatcaag cacagtatgg tgtggctatg 54480tagaggaagc atcttcaata aatgtactgc agtggtaata tgcttttaat aaggcaactc 54540tgttcacatg aacagtacta gagagaagca caccaggccc tgaaacttca gggcaaacaa 54600aggtttgaaa gtatccctga attaaaataa ttgaggaaag gtgacaaacc taagcatgtt 54660tgggtttttt tctgagacaa gcatcgtgta ggttgttttg agccctagtc acagcctggc 54720aaaaggaacc tggtgcagtc actatgggca ccagagagga aggaagaaca gtgttgtccc 54780tgtccttgtg agaaggagct ctgaaccaca gtgcacatgt gtgggggttc acaatactgt 54840cttcctggaa ggactggatg cttcagtggg aagtgattaa tccaaagcac tgtctctctg 54900catctgattt atctgtgcca taccaaggcc agttatgccc agtgctaaga gttgggagca 54960atgtctttag ggaaaaggtc agatgccaaa tgatctgatt ccagcacttt catttcatct 55020ttcatttcct gttctccagg ttaaaggcct tgttctcact gaaagctggc aactgtttgg 55080ccgcctgtta tttcagagtt gttttatcat tattattatt ttcctgatga gatgtatatc 55140ccaaacaaga acaggctcaa taaaataaat gaatgaaatt aatttcctgt ctttgattag 55200aaatattcac tggtgaggca cacttctacg tcagcagaca tgtctgcaga aggctgagtt 55260cttgctggac gtgttgaagc agtgtgttgc tgtgtgacac catccctcat ccatctcagt 55320gcagatgcct tgggaagaaa gaggaaaaaa gagaggtcag cttgctgctg ctcagcttgt 55380gtctctctca gtataatcct agaatgacac ttgattattc taagtgctat tgtagttgca 55440aatcatcgtg tggtttgtaa ctgtcagtct gacctttact agacatatac tggaaaatat 55500tcttgcctgt gacttctctc tattgctaaa taatgatcta gacagataca cagtgaatac 55560agaaagttca gttgtataga ccaactgaca gacattgtga ttttaccctt tgttttttct 55620aagtgtgccg aggaagcagg ttgttgtatt gaaataaagg catgcaaata acctgctact 55680ggctccctcc aagatctcag gcttgctgta aaagccgtag ctaggtcaaa aggggttgca 55740ccttttgtga ctggcagcat aataaacatt ccccagttta ttctgctcat tattccatcc 55800cacttgtagc caatttcctg tgtggtctcc aaagcatgaa atctgcaatc agacatgtcc 55860tgagtgtcaa tgcattaggg aaataaaata aggaaaaaaa gacaacagcc gtcagttgga 55920gtctgtgaag gagctgagct ggttcataga actgtgttga gcagcaggag cgttccttgc 55980cccaaacgag cctgtccaag gggtgggagg aaggaagctg tttttctttt ccactcagca 56040gcgtgtagca acaacgccct gaaggagtgg cagggtgagc cgagacctgg ggctgagagc 56100agacaggatg acaggagtgt taccaggtgg ctgcatccct cctgcacacc gcatggccag 56160gtggtggcac atggggatgg ctgctgcgtt ctggtgccca gcaaggcctt cgcttgtgtt 56220tccccttggg ctgttgaaga cctgaaaatg ttggtgacct ggaaaaggaa gatgaaagct 56280cctctgttgc tttgtcagaa gactgtggct tgtccttgtt atgaaacttt ggactgcaat 56340aatgacggtg tttcactttg cacatccctt gttcctatgt tgttttgcct ctgtcttttt 56400tcagagacag catcatgaca ggagatgtcc aactcacagg gagctttaaa acagctttga 56460ttttattatt attattatta ttattattat ctagtaaaga aaaatcctgc tcttcattct 56520gctatctttt taacttgatt aaaaaacagg ttgcaataga tgtgtgttga aaattcttgg 56580aggtcaaacc aagcaagact aattcggtga caggtaaatg caggagatgc acaagctgat 56640gcagttagtt agatgtcatt cagtcagact gaggaagatg aactgggaag ccaagctcca 56700gtgcttgtcc cttgcaaacc tcaggtacct aaggctcagc atcttctgct tttcaagtca 56760gcatcttttg ctttactgct tcttcctggt tgggccataa gtaagatcca ggtaagtgac 56820aggcactccc ataaatacta atgtgaagat acatataaca tatacataaa atgactttag 56880gatgttattt gtctctatat gtgacaccta atattatttc aacattatct ccaactgtaa 56940attaacccca aatatccatt ctctggggaa gcaggactgc cctgtgagcc actcagttac 57000aggagcccgt gtggactcct ttctttgaag catggcaatt ggttatttct ctcatggcac 57060tatgatcaga aatcagtcat ttctcagcag cagctgggtc aggcagaaga gttctgtaag 57120cctacaaaat gccagacctc agctatcaaa gagaagttac agagtggagc gcagtaatgt 57180tcatgcacct tcagctttgt aatgtagagt tctttcacct ttccctgctc agcttttaga 57240agtaaccagt gcaccccctt gtatttgtat ttccagccta catggtgatg gagctggtga 57300tggtgatagg aggtgaaggt ggtgacctgt gctatctctg tcaaggcagt tgagttgtca 57360gccactcccc accagtgtgc atccagaagc tcctgaacca cttcacagag ggccgtcttt 57420agtagacttg ggcctgaagg aaagtttgtt cctttggctc tagattacct gcagacatct 57480gagaagagtc tggcagccaa cattattgca gatttttatc aaaatttctc tcatagtgta 57540aggcataatt agatatgtga caataattgc ccagatgaca catttttgcc agctgttctc 57600gatgttcttt gaattgctta aacatatcca caccttaatg tttgttatga attcacttat 57660ggtaaagcat actaatttta tgttcactgt ctggtaaata aatgaagttg aaccctactt 57720catcacagca gaaaggaaaa taagatttcc tttgggtaat aatgagtctt ttggaagcac 57780atattgctgg gatctgatca ggaagcagtg gtgtgattta cactcaaaac tgtcattcca 57840gaagtagata actcctcagt tgccactgcc accaaggagg tatcacatca gggaagacac 57900gtactttggt ttcctagccc tttcatttgc cagcatagca atcttgaaag caagctcaat 57960aacgtttact ttttttgtag cacatcagta gtctaagggc atatagggct gcctggggtg 58020ggtgaggtgt ggacagcagc tgtgtttgtg ggtggcagtt tcctcatgaa ggttgacttg 58080gactccagga agtccttgtt agtgtggtgg gcagctagac ttcgtgatgg gagaagcagt 58140gtggagcaga gtaggccacc ttttccttgc taaggactac atacattttt caaatcaaat 58200aagactgtca ggctgcacca ccacctttcc ctctcccttt cttcaacccc ctccgcattg 58260gattcatcag taatcatgtg gccaatgagg cctgaaccca gtggttccca ttctggagat 58320gctggaaagg agctgtggct ttttccaccc tcccagctga aatcatctgc ttgagagcaa 58380cacggtgagc agacatttct ctttgagcac gagccccact ctggccttgg gcttttgagg 58440caaccaagta caccacacaa ccctcacaaa gacaacactg agacactatc ttaacgcaga 58500cacagatgca aagtactggg ttgccatcac acgttttggt tgccttccct gtgtgtcttt 58560ttcacaatta caatttttta cgaatcacag caattccaat gtaccttgga gcactggttt 58620tgctcagaag ctccttcagt tgctccttca ctcctgcacc ctctctttca gagtctttgc 58680tccctcctgt ctggtgccta ttgtagggca cttgctgcag gcaacgtgcc gaggctgctg 58740tttcctgctg gagcatgttg ttgtgttcag tagctaggca ggcaaagaca actcaggtgt 58800gactcatact gctcctccag tttaggagtt gcttgaagaa tgattaaggg aaagaaaaaa 58860aaaaaaaaag aagaaaaaaa aagcgagagt gtgtgcgtaa ttagggctgg gaaagtggga 58920gtggtgtcaa tgagcaggca agctaacaga ctcttctgct cagcaaggtt tcgccacagg 58980tctctagttc ttcgtgttgg acccatttca ttgattcatt taccaggatt atacccttgc 59040aaagtaagta caacattttt tcttcctctg gtactttgat gcctaactaa cagttataaa 59100atctctgccg tgaaacgtaa tcgcatagag tgagcaggat aaggaagctt gtacaaaaag 59160acagcatgtg aaactaaagg tggaaaaaag cctgctcaga ctacaacctt ctggttttgt 59220cccagcattg ctgtgtttgt ccgtctacat tccttacccc tgaaaagatt cgttgcaaac 59280ggtgagtggg gtctgtcagg tgagactgcg aagacattag gaattattag aatatttaga 59340ctacctgttg gctgttccca atggcttttc ctgaatcaga agagcaggct gtatatgatg 59400ccatgaaaat gctccatatc tctaagtagg tgtagactgt ttgagaagtt tacaaaagaa 59460tattcttctt gttttccaca tgggttcgta tgctttgctc tgtttgcttt gtctaagcct 59520tgctagttca aaggacaaga acttaagtct actaattacc tacttgatct tcagtgtgct 59580catccggttg gaaaaattca ctgactcttg aggcacaata aagggtattg tggagactct 59640ctaattcctg gtgtgacttt ctcaattgtg ttgctgatgg tgctttttcc acaacctgat 59700gaacactctg atctcgctaa agcaaagcat cagtctgata ttgtgtgttc ctcagagaaa 59760catctgttca gaggaaatca tgtcttagtc acggagctgt gtaacctgcc tggtggagag 59820ctgccatttg tgtagaagta ggaggaagag gctcacaaga gttttgttcc tttatatttt 59880gtgttatcca agcaagagct ccagtaaggt catgttaaat gagctagttt ggagggggaa 59940tgccccacat gtgggttctt tcatatcgtt tatctaaact gaagtgactg cagggtgttc 60000actcacagct catgagctgt agccctagtg cacagcccca atagcagccc agcttggatg 60060gccaccgccc ggtctgcccc gggtgcgcac tcctcagggc tctttaacaa aggcaagaat 60120aaaataaata cttgctctgc tttatcagat gatgcttacc attcagctga cgtgacttgt 60180caggtttcca cacagatgtt gccgttctcc tgattaatgt

tcagaagata aactacattt 60240agcttttctc ttagtaagca aatagcaaac aaagctttgt ttctgttggt tgcattcagg 60300agtgacaaag caaaaatagt gtcctatact actaaacacc tttaagttat tttttttctg 60360cactgattct agagcctctc agcttcctcc tgtatctgaa cgtgtttctt gaactctgtg 60420gccccatcac agctttaagc aaagctgggt ggatcacagg ctgcatgtgc ttagaaggtg 60480ccaccgtgcc gcgggcctct cagaatgctg acttgttgct ctcctgggaa agcagggatt 60540cagccagaat ccaagcagcc cttcttgaaa tttcatttcc aattttgttg actcctccct 60600gtgtgagagt ttcctgtgat tactgactca ggagctgtgt ctggtttctg ggactgctcg 60660tgggcacctc atgggctttc gtcttgagtg ggggcctcag cccttctcac tcagccagaa 60720cttgctgcag tggggtcact gacacagctt ggggtgctca gggctttaaa gaggttcaag 60780acttcgtaat atttcatgca gtaaattcct ttcaagcatg tgaacgctgt gagctcctat 60840gtgttgtatg tcattaatga atgcagcatt aaaaaagaag gctgatcaga tgcagttaaa 60900aaagatggtg agatagagat tattctttgc tatccagccc ttattgaaac agcagggtga 60960aactgagggt gtttttttcc caacaaaatc ctctgaatgt gcaatatatc agtagcagca 61020ctaaaagaaa gaaagtgata agccttgcca ctaccaggaa tagattctct tggcataaca 61080aaggcattga gaagcatcat cagctactga gtgaacagga ggactgtaaa aggttcacca 61140cgaagtacct ccaggtttcc tcactgaaga ggaacacaga aaccttgcaa aaacgatcca 61200gcttgaatgg taccagaaaa gaatttctac gtcctggtgc agaattccac tggtgtaagg 61260aagaagagag tcatttaagt ttgcaaaatt tcacaattta tttccttgct ctgaatattt 61320tgccacccag gagagtgaag cacaggtagc acatgcacat tttaatatca ctgtaggtca 61380tttgccaata cgactgaaaa tgctgatgtt agaaaggcag gattgcattt ctggcatgaa 61440gacagaaagg aacgtgaaat gttttgaagt tattatgatt gcatatattt tcttaggcgg 61500taaggaagat ttggaagtca aaatagcatc agggcagccc taactgaaga aggatatttt 61560actccgctag caaatgaaat atttttcagg tagactgcac acatcattct ggcattgtga 61620gattatgcgt gttgtttatc ttcacgagag tggtagatgt tgaatgacac attcttggtt 61680ccttgggtaa ttttccacgg tctccccagt gagaaatgcc tgggaagttg gtacttgccc 61740atttcttcca tttttacttc agacagagaa agtatgcata tggattgtgt gctcgtgggc 61800cttaaagtgc ccttaaagag aatgagttca aagggaaaaa taaggtaggc atcctgttca 61860gagcagtttg tgtaaggtgc acagaagtgc gtgtctgtgt tgagcgagtg cagaaaggca 61920ttttaaagga tgatttcaca tgtgctcctt tgacctgttg ttccaagtga ctccctcagc 61980agcagtccca ggtcttctta tttgttttca ctgtcttttg ccaccatttt gcccaaagct 62040ccctcctcct ttgatgtatg cggagtccat cgtttctagc aagcttgact tttctggtta 62100ttagttgctt ttatatgtga gaagttgtga ccacaggagt gacacaggaa tgatgcttgt 62160agtgctgact ggcactgagt tctcactttt acacccagaa aaactctgag aacacttccc 62220aaacctcact ctgacaccag cttgattcct gctgacactg taaaatggga tctcccaggg 62280taagcttcgt taccaagcat cttgggacac tgccagtgtc aagggagatg gacagaccca 62340ttctgcttga aaagcatctt acagggatcc tttacatgtt gtaaacatcc ttcttttcat 62400ttttatttgg ggataacttt ctctggtgct gtatatttaa ttttttttcc tcctcaagat 62460gaattgcttt ctttgcgttc ggaggcaatt aggaaatact ttgttgctga taccaacagt 62520cagagcactg tgtgagggca cactgctggg taagtgtgtt tttcaaattt ggatttaaaa 62580agtcttgatt ttatgccatt atcctttttt cacttaatta gattgtgcat tatatttcag 62640taaccttttg tacagcgtct tttagctaaa attaagccag gtgccttact aaatatatag 62700aacatatacc tatgtaagtt aatgaaaaca aagacgtgaa ggccttttct aatcaaacag 62760attttacatg gaaatcaaag ttttctcagc tgtgttgcag aaaaaaaata cccccctgtt 62820ctgttactcc tataaaaacg tgtgaatacc acagattatt ttggaaatct ctactctcaa 62880ctaccaaaac tgccacagca tctcgataca ttgatgtctg atgttcagcc aagtttggac 62940agtatgacac atgctcttga atgcagattt ttgtcattca aaacaccatt ccaaacaggg 63000atgagagtga gcggtcagaa gcaggtgtcc ttgctctgga gacagttccc tgcccacatg 63060tcccctcttc cctttcctgt cttctcttac ctaactgctg tcatctggtg agatctttac 63120tcatctgatg caacctagaa tgcaaaaggt atgaactagg taaatgttta agactgcagt 63180attaagtagg catttgagag aaatctctgt ccttaaggtg cttcttggaa gatcagcaaa 63240cctctcaccg aggtaatgct tcagataatg ctacagactt tcctgtttgc gtcttctgtg 63300tcagagcctg aaacgttatt gcaaatagat gtctggataa gaacagaact gttaaaatca 63360ccttgccatg ccatataagt tccaatattt tgccattttt tttcctgggc agggaacatg 63420ttgaagaaag tttttgagtt ctgttggaag tctttccctt ttgaagtccc ttgcagtatt 63480catcttttcc ttttccttct gtctctttca atagacagag ctgctgagca ccaatttatc 63540agattgtctt tccccttctt tagggacatg tgattctggg gatagaagac agtcaaactc 63600actgtgccaa aggagttacc gtcttccata tttgtgctgc tcttaagctc gatgcgatat 63660tgactgaaat tctgtggttt ccctttgttg tctttaatct acaccaatgg agttacaccg 63720aagtgcagtt ttagatctat gaaagcagtc tggaagatcg aatattccgt gtcattccca 63780gaacgtggtc cagaacatct gtcgcttggc accacctttt ccattcctga ctgcatagat 63840cagctaacag ccctacggca attgcagtta ctctgaactg ctaggaaaat atttgcagtc 63900atcattgtaa gtgatgagtg ggcacatagc agtatttatg taggaggcta agtacttaga 63960gtttctagga tgatctcaac ctacaggacc ggacagcttt ctggagagtt ctagcaaggg 64020taaggagaac agggaatcac ctcttagaga gaggacatgc cacagctaaa gctttaatga 64080acaattagat gtgaagcaag agacaggaaa gatgattgtg agacttttaa aagcctatca 64140aagcactagg agagcccaaa gcataggcaa agtaccttat aagttggcac atctgaagag 64200tatcaattaa aaacatatta aatccatatg ttatccgatg tgattcaata tgtgtgggtc 64260accctgacca acccagattt ctccacgtat gtctggtaat actggctcta cgtagcacgc 64320agaactgcca gctgtcactt gaaggtaagg gcttctactg agccactcgc attaccttgg 64380ttgggcatgg atgagagact cctcaaaagc tgctggtggt gtctgagact gggcaggatt 64440ggtcaggcct ttctcgcctc ccagcgtagg ttcaagctgc ccagtcccca aactggtgtc 64500cagcctcctt cagcaaggaa atcagtgacc tgccagcctc actgcaacag gagctcactc 64560tgtgggtcat ctctatcctt ttctgtttca ggatgacgat ggatgctctg caactagcaa 64620acactgcctt tgctgttgat atgttcaaaa agctatgcga gaaggacaga acagccaata 64680ttgtgtttgc cccactgtgt acctccacat ctttggctct ggcatataaa gctacaaagg 64740gtgacactgc agaccaaatg aaaaaggtga gctgtcggca tcctgctgtg tagctgcaaa 64800attgtcagag gtggctttcc tatttattcc tcttaatgct gtataggact gctggttccc 64860ttgtaagcca ggcagaaaac tgtccatcca aaattccaga atatttcccc actccatggc 64920tccacacaac caaagaggct gaaaatcact agcataggga aaaaagcttt ctcaagcatt 64980tacaaggtgg atggggacat ggcagagtcc tcagcagttg tattaaggcc ttgtctcctt 65040tcagcaggaa tgctgattgt ggctgaaggt gactgctgaa gtcactgcat tttctggata 65100attgtttagt gattattcag gactgcctaa gcttaacagg actggaaata attttgccat 65160taccaagtaa ttttagcagt tctgtctgtg ccatttcccc tttctcctgc catacagcta 65220agaggaagat aatgcagtag gaggcagctc agcttgagta gtagtttgcc ttgcaaatag 65280ctctagatgc tcaagggttt tacagcacca cgaagcagca tcatggtgat ggtgcaatga 65340gtttatcaag gttgctctgt ggcggtgaga ggctgcacga ctgcctctgt gagagccagg 65400atttacacag cctcttttta ttccagtgcc cacagtctca gcagttacct agaggtgaat 65460gagaagcaaa ttcagcatgc atttatatgc tgattatcac ctggctctca ggggcattcc 65520atgtatttga atacattttt cttcgtttag cagttcctcc ttgtaccttt ggtttccctg 65580acggcacatt gctggagcac agcctctggc gcctctgctc atcctacaga ttgcaatgag 65640tctatttgca cagaaacaaa gtggtatatc cacaaaggcc tgctgggtgt tttcccaaat 65700aggattattt taaaaaaata aaaataaaaa tgatttttag atcttatttc tagtttaaat 65760gacaccccaa agcttccttg tcatttcaaa gttcaagcac tgtctttgca atggaagagc 65820ttaaaacatt aacctgtgct taatttcact ttcacttgtg cctgcaattt gcattgaacc 65880gtcccacaat aagtgaacat ccacatccac aaatagggtt ctgttacaca agtgcactta 65940tgtttcacat ttctcaaggt aatttactgt gcctgtaaag acatggtgtg ttcagggaga 66000aagagcagga gtgaggctga aagggaaaag gaggtcactg atgctggttg ggaaagatga 66060gaagggttgg gcaggctgtt tttaatggaa catgcactct cagagacctt gcaacaggca 66120ggcacctaaa agcagagagg tttaggtcat gctagaatat cctggaactg ggcatgtgat 66180ttcccggagc tgggaggtgg gtcagcagcc ttacctctaa cttacgttct gtctgccaaa 66240gctcacctgc ttatctgact gatttctact gaaataccac atgacatcat gtgtcaataa 66300tcagaaaacc ttgccatatg gtaagcagtt tttaaagaag taacccactt ccagaaagga 66360aactaactgg aacatttatt tatctggcct ctaaactcca gatttttgga caagaatgtg 66420agtttgataa aagcatgact ccacgctgca gatatgtagt tcactaaatc actttgctag 66480tatgaacagc tctatggaat tctttggact gctcacagga aggaaacaca tttggttaaa 66540gttttgatag gatcaagttt ttagatttat gtggggatgt caaataaatt aatttttttt 66600ttagtaataa ataagagtga gaagtcgtgt tgttagcttg aacacaaaaa agtcaaagct 66660ctggtcacaa acaagcatta tttattgcca agctgtcagg cctggagcat gtccagagaa 66720ggacaacaaa gctgtgaagg gtctggaaca cagatcttac aggagagcag ctgaggaaac 66780tgggattgtt cagtttggag aaggagaggc tcaggggaga ccttatccct ctctacaact 66840gcatgagagg aggctgtggt gagcttgggg ctgacctctt ctcccaggta gcattaatag 66900aatgagaggc cgtgtcctca agttgcacca gaggaggttc aggttggata tgaggaaatt 66960tttctttttc tgaaagagca gtgagatatt ggaacaggct acccagggag ctgttcaaga 67020actgtgtaca tgtggcactg tgggatatgg tttagcgggc acagtggtgg tgggttgaca 67080gttggactag atcatcccag aggtcatttc caaccttaat gatactatga tgctatgagt 67140ttttagataa taaaaagaaa ggtgctcagt attttatctt gttcattatc aggtgctcca 67200tttacaagac gtcaaagatg tttcttttgg gtttcaaacg gtaactgcag atgtttccaa 67260actcacctct ttctttgcac tgaaaatggt caagcggctc tttgtagaca agtcgctcag 67320ccctaccaca gtaagtactg cagaaaagtg cttgaattgc tcgaccaacc agacttcaat 67380gttattcaaa atacgttctc tcactattag cttttacttg actagactca gatgatgaac 67440agcataataa gagtttgtag gaggatgatt gttctgcttg accccaagca atgcagccac 67500tgctagagtt gcaattcttt cattaatatg ttttaggtca gtaggcgcag taggttttga 67560atgcaatatg acttctatgc cacatcaagg gctttgcaat ataagtatga ctgggaagga 67620ttttaaataa agatggtggt gcaagtgtgt ctagtccaca cacccaagta attactgcat 67680aaagagtagt tttcttaatc taactgagga ggcacaagcc tggttattca aacaacacaa 67740gtgaggaaag tgttgtttgg ccatgaaact taaggacctt gcaaacaact gagaaaaatg 67800ttgtgtttgt tttatcagag ttgcctttga atagggcccc aagcaagggc aacttcagcc 67860tagaagtgat gtttcagaag actcacagcc tgcttgaatg gtgttataat caggttgcct 67920gctttttggc cccatccaca gcagtgagca tctcacctga caaggatagg cacactgtga 67980gcagcctgtg gcctttgtct catccctttc ttttgcccag gtgtagactg aaggctactt 68040tatcctttca aactcaggca acatgttcac tcctgcagta cgaaaggtac tttagcagcc 68100agtataactg tattgaagac agtcttggga gcaatctgct gaatgcggct gcgtgtcctg 68160gctgtcacct gctgttactt attagctgtc ctttgtaata tactctctgc ctacaccgta 68220atgaagcttg ggatactggt tttgtaggcc gtgtggagag tcatctagtg aagaacatct 68280aaggaaggtt agctttggta ccttgtgtct ttcaggactt tgttaactcc acaaagaggc 68340cttttccatc agagctggaa ctagtggagt tcaaggaaaa aactgaggaa acacggcaga 68400agatcaacaa atctctctca gagctaactg atggtgagta gggcctaacc tcggggatgc 68460tgattacctc tttgaagaat gatgtctttg tcttcatgac atctcctaac tattgctttt 68520agaagtaaat atacagtgaa agcaaaggga ctgcacctat tatttggatt catgaggatt 68580agctgtgtta gcatgtttta aaatcattta ctttactact gtggcatttc tggaggcaga 68640ccttacatta gcctttggca aagcatctca tttgttttca ttgggaaagt ttggctcctg 68700gctgcagagc ttcacaaaca tctgacatca atacatcaaa tcctggcccc gttctctaat 68760ggagagtatg tgctgaactc tgaatttcag gctgttaatt agtagctcat ctcagcagca 68820cagctgattt tgaccacagg tggacatgtg tttcttactt ggaaacactc ccgtggcaat 68880agttctgcag cacttttcct gcagtaccac tgagccacta agtcacaaga agtgcctctc 68940agtgaccatc aaggctccca ggcagaacct gcccagtctg tgcagggtag aggtctggta 69000cgcagtgccc aaggcagagc tcatgtacat gctgtccata ggtagctcca gggttggttg 69060ctgcctattg ccctcatgtg gtacacatat gaaaatatgg gtgcctgagt tacatctgct 69120ccatcccgag gtgacacagg tgcccacagg gaagtacttt gcgctgcctg tgtgatttgt 69180gcatgaatga agactaacat ccacaacact gtggattcag tgcctcatga cagtgtttga 69240acagacacaa aataaagcaa gggaaagaat tacgttctct ttttgaaatc catggcacta 69300tttggttatg aactgtaatt agatggttag cggcatttct tattcgggtt tattcttatg 69360tatcactcca aaagtgagta gaagctaaac tggaacttcc cttgaagtct cgctctccaa 69420atgagaaata tttttttcag ttctacctgc tgaatttcgc tgaagtttca gtaccttctt 69480taaagtacta aagaaaagca gtagacatat tttttattct gttttatgta aaccgagtaa 69540aaatgtcact tggaagatct gtcttgatcc caaattccat tttaaacatg gagctgcagc 69600taaggaacta aatgcttcta tttggggatt tccctttata attaaaactg ctatctgtga 69660ggtgcagggc agaaatattt taattcagta cagtgtttcc atgttctgtg aaaacagcac 69720atatgttgat aatttactgt attaatgacc agcttaacca tcttcacagg caaaatggag 69780aatattctga atgaggacag tgtaagtgac cagactcaga tcctcctagt taatgcagct 69840tattttgtca caaactggat gaagaagttc ccagaagcag agatcaagga atgtcctttt 69900aaagtcaaca aggtacgtcc tgaaataaaa tagagtacac cttctactca gatgaatgtt 69960tgccaatttt gtgctaagga aatttcagtg agagcaagtg aaaaatattt gttactacta 70020tggcattctt agactctctg tcaaaaccta tgtgctgttg caaaagtacc taagccagtt 70080ttcttgttac gttgctagtt tgaagctgtt ggtgaaacaa gcactaaagg tcaccgatag 70140taggtaattc tttcctttaa agcacatccc cagtatattg tattaagtac accttgtcac 70200atgaaaactg ctccccttaa agtaccaaca gctttcacta gcagtcttac agctgatatc 70260gttacttaca gaagccaaca aattccatga tggtaatcaa tgtaccactt tcatgcaagc 70320ttgcaaagtt tcctctctca tcttctctgt gaattaaaag gagtgctaga ttgtctcctc 70380ttgtgttttg cagactgaaa ctaagccagt gcaaatgatg aatctggaag ctactttttg 70440cctgggttat gtgaaagagt tgaatgttgc aatccttgaa cttccatgcc ttaacaaaca 70500tataagcatg ctcattctgc ttcccaaaga cattgaagat gaaacgactg gcctggaaaa 70560ggtgagagaa aaaaacagta ctgagatgat gctttccatg cacagctgtg tcggttagct 70620gtgggtagct tgggtaggga ctgtcttcct tgaattcctt cattgggttg ttgagctgat 70680tacatagcaa acgcttgtga agaaccagta atcagagtat gcacatttag tggagtttct 70740ctggaagtct actctatagg ttaaataatc attatatcaa tataactgag agtgtaagtt 70800aactctgaat gctacaagca aaagttgtct tttggacttt gtttttttgg ggtttgatag 70860gactgatgag ttcagaaatg gtctttttgt tcccactttc tctggactgc acattaattt 70920cctttgttct ttatgtcctc agctggaaaa ggcactcacc cctgagacat tattacagtg 70980gaccaatccc agcatgatgg ccaacaccaa agtgaatgtg tttcttccaa agtttagtgt 71040ggaaggcgat tatgacctga agccactcct ggaaagcctc ggcatgacaa atgtctttaa 71100tgagagtgca tcagatttct ctgagatgtg tgaaaccaaa ggtgtggttt tgtcaaagat 71160cattcataaa gtctccttgg aagtaaatga acagggtgga gagtctctag aggtaccagg 71220atatcggatt ctgcaacaca aagatgaatt taaagctgac catccgttta tctttttgtt 71280taggcacaac aaaactcgca atgtgattct ttcaggcaga ttctgttccc cataagcaga 71340gaatattaat tatgaaaaag accataaatt tatggtgatg catgttcctg taaagcttgg 71400tgtcctgact atcacctttg aaaggaattc taagaggttc atatatcaac agggtaatac 71460aatgtactct acatatgcag cagaactagt ttatttcctt ttatttaatc cccttaagct 71520gaaggattcc cactgtgcag aacacatgat atttgactaa gaagtattcc atcctcatcc 71580acgagaatat tttgttcctc tgtgacatct ttttccaaaa caaaatgaac agagaacctg 71640tttttgaaag actaggagct ggaagaggct ctgggggaaa gagctgcatt cctgtttcat 71700atccaaaaca ccttcccttg agactcatac tcactgccta aagggggaaa atgtggacat 71760gtggtgtgat agccctcctc ttgtacttgg ctgtagtctg gtgatccagg gtgccctgct 71820ggaccctgct aatgcacggt gaaatagtgc agctgaacaa ctcagagttt gcatctgtga 71880aacagcagct gcaatatgga tgcaagaggc aataataaaa cacccagaag actcttcagt 71940gtgtgctacc tcagtttgta ggttggggag gttgcactct actgtgtggg attttttcac 72000tcattctcct tcagacatgg cagaggtgac cagttcactg cagctgagag gaactctgtt 72060gtatatatcc tgagaaaaag aaggctgtgc agttctagga tagaaatcac ttggattaat 72120attgaaaatg ccacactctt cagatacaga ttttctgtca cttctggatt cagcattaag 72180gaggctccac acgtctacct aacctctggg attcaagaaa gaaataaagg ctttgacttg 72240agtgagatta acactgtaat tagaagtctc caaattccat atgaaattat ggtaattagt 72300tttcctttct atctaaagac tgcctgctgc atatgttcag tactgattta cctaattact 72360gttcagaata aagcactaca aaacctgtgt caaatgtctg tagcacatcc agtgtcagtc 72420tgttctcctt cactcagctt ccaagagggg ataggaacag aaattggtag attcattcag 72480gacacagtta aaataaatat atgaagaaat taaatctgtg actgaattgc ccttttggac 72540cacacgataa tagctgacaa ttaaggagta tagtactatt tggtcaatat atagagtgag 72600ttcaattata tatcttcaaa gaaggggcca ttttaactga gtattcccct tggttcttca 72660gatctgaaag aacctagaga tttctaaatg ggaacataca acccttaata catattcctt 72720tcttctcata gcagagaaca gcacagtggc tattatggat ttggagagag ttctgttttg 72780attcttggcg ttcccaaccc acatgtagcc ttcagtcaca aacgttcagg gtttcaccag 72840ttgtcttccc tctgtgagtc tctaatgctc tctgacttat cttaatatca gacagttagt 72900ggatacattg gttcacatct ttgaaggcac tgaggggact aactcaccaa aaccaacaag 72960agatgagtcc ctgcaggcat ctgggggtct ctgctgctct tcatcaggca ctctgataca 73020tagatagaag aatgctatgt gtaagactta atttcatact gtctgagagg agacagcctg 73080caaagacctt tactagctca atagcttcag tgataaatga gtgtctggaa aatgttttaa 73140tgtgtcccat cattgtctga tctgttttgg aggctgcggt tggagttatt cagagctgtc 73200atcactgcgg tgtcccctgg ttctcccatt ggtctgggca ttgcacgtgg ggttggcagc 73260cgatgagcag ctgggcagtc tgtacatagg caaggtggac tggttgccta cagtctgtgc 73320agtttctctg tgtttcagca ctgactcgtg gtctagtaat tgcaggtatc cagcaagcca 73380aatcacactt cattgctact gctgtgctgg tctgctagac tgatgaaaat ctctgttaga 73440ctgcctcatc tctctttttc tgcctggata agacttatta aaggagaaaa gctgatatat 73500cactctcaga ttttctagat cacgaaaaca ttgcagtgca ggagccattc aatccagctc 73560atactccatt taaatgctga tcagaacact tagtgcatcc acgtacgttc ccagagagct 73620ccttgttggt gcctttgcca aggagggcta tcccatgaaa gcacacagga atgctgcctc 73680cggcagagac ctgtctgtct ccaccttctg attaacacaa aggtacccaa gactatgcaa 73740ggcaagctga tatttcagat aagaagttgc ctcagtttac agaaggccat gaaaagctct 73800ggttcatttt ccatatctgc ttcttctctc tgcttttgga aaataattct tatttcctac 73860tagcaagtcc agactagtgt aatatttagt cttgatttgt taaatcatcg tgaattttag 73920cttttactaa tggtatctta gatgatctaa tatactaaca cctagtaagt gacttcagat 73980aaggtgttag gcttatacca cagcccagtt tgaaggagct aagtgcagat ggcaaccaaa 74040caagcaacaa cacaaatagg cccaaatagc ccatggaggg ccagaaagta cagctgcagg 74100cagagctgtg atgtaatcca tttttgtgag ccccttgaag ccagcaggcc agcctcgtgc 74160ttttagtgta atggacatca tacagccaaa aaggaaggtt atgccataat ccttgctcca 74220ttcagcaatt ggactcgagt gtactagccc tgtttcctag acccttccag tcctatggaa 74280atttagaagt cccaatgtaa aacctattca acttgcagca tgtgagagga agatatcaga 74340acagctcccg ctaacgtgag aaatgccatt acagggtact gtactgttcc acccattctg 74400gcctctgggc tgtgggcaag aaactgcatg ggaggacatg ggaaaacctg tgtgggagct 74460tgcatataga aatgtatttg tactcaaagg cgttggctgt gacggagaaa gtgagcatag 74520gtgaggactt gctcagatca ccaagcaacc ccacctcacc tttcaacaca gcattactat 74580ccaatgatgg gtaactggcc tgatggaaaa aagcaagcca tggctggctt ttgcaggcag 74640ttgcaaactg cagtgttatg cagtctagcc atctcataac ttgcttagct gcatagtcat 74700tgcccacttc ctgctcccag cattttgtag aagagaactg gtacatctaa atgctttgca 74760gtagcaggaa ttggttttgg agatgagcag ctggttttga aacttgaaaa atgccacata 74820caggcaattg gcctggctgg aaaagcagtg cggttttgag ccttaggtat gttacatgtg 74880caagtgtaga gtcctacacc tggggaggaa tgactgcagg taccagtaca ggttaggggc 74940tgagctgctg gtgaggagct ctgtggaaaa gaacctcggt gttctggcgg gcaacaggtt 75000ggccatgagc cagcagtgta cctttgtggc ccagaaggcc aatggtatcc tggggtgcat 75060taagaagaat gtggtcagca ggttgaggaa ggtgatcctc cctctctgtt ctggtgaagc 75120cacatctgga gtactgtgtc catttctggg ctcctcagtt caaaaaagtc agggagctac 75180tggagagaga gtccagcaga gggccacaaa gatgcctggg gtcctgtagc atctcccttg 75240tgagaaaaga ctgagaaacc ttgggttttc cagactggag

aagataaggc tgaaggggga 75300tcatatcagt gattacaaat acttaaaggg cagaagccaa gtgaataggg ccaggctcct 75360tttggtatcc tgtgacagga aatgggcgaa aattcaacac caacaagagg aagtacttct 75420ctactttgag ggtaacagag gactggaaca ggctgcccgg agaggttgtg gagtctcctt 75480ctctggaaat attcaaaacc tgcctggatg ctttcctgtg caacctactc tagggagctt 75540ctgtaggagt gagttgaact cagtaatctc cagaggtccc ttccaacctc tacaattcta 75600tgattccatc ctaacggcct tagaagggtc agaatttgca catacggtat aatgttctga 75660ggcaaagcag tgaaacagat tgcaaagaag ctctaaggag atatagagag caaatcaaag 75720aaatgagtgg aggaacgacg tcagtgtaaa agggagggag aaagcacaga gtttggaaga 75780acgaaagcag tgaaaggtat tcaaaaatgg cactgaaaag tggctaggac tcacaaaagc 75840agcagaagaa aaaatggaga atggaatggg aaaggcaata ggcagaaaga aagaaaaaga 75900taaagaggca ggaacaaatg actaagaagt ctgaagaaat atgcagaaag gaaaagcaaa 75960caacaaagcg aatccaaatg gaacagaaaa aaagtgaaaa gaaggaaaat attactgagg 76020agatctgata ctgtgtgcag atttgtgctt cccatctctt tctttgcatc tcactgcatg 76080ttggggacat aaccccatgt gatcagcctc tcttggactc ctattcttag ctccagtgat 76140ttaaaagaat accccctggg tggggattcc tgttacagat caaggaaata cttcttcctc 76200tctctaatta aaatccttcc actcacagaa agctggctct gtacctgaat gcgtgcctac 76260tacctgtagg caacataaag ctcatgcatt tcctattcat ttgctctatt tctgcaagca 76320agctcagccc caaacaaggg atctctaaat cctagcaaga accctgcaca ccccagtgtt 76380caagtcctga caccaccaaa ttcaaaagga actacacaca gcaccacagc catggatctc 76440cagtgttaat gctgttctcc agctaagggc gacttggctt tgcagtcagg agatgttgcc 76500aggatgcctc ctgtcaaact agttgggcag ctttgagcga aatgctgtta gcacattgct 76560agatataggt ttcctggtct tctgcaggaa actgaaggat gacatttgca tgaaattaca 76620acgtgcagcc tttatcaaca attggctaga gactgaattt tcccacaaga aagtggaaga 76680aatttaaaat agagtataca caggaaggtg ctccagagct cagctgttgt gttcttcatt 76740tgacctcctt gctcaagaag gtaaacatta tttttctctt caaaaataac ttgtcttgtt 76800gttgttgttg ttttggccaa atcagtctaa aagttggtaa atttcatgtt tatagatggg 76860gcaaaggggg aagtactttc acaggctgga gagagcaaaa gacactgcta aaatttgggt 76920ggtcttcacg aagggaggtg gtcttctctg gggtgaggct gggaatttag gaacacatgc 76980ccaaagctat gaatctaaag atgcctgtct aaattcctca gacttttgac tgaaatttcc 77040ctcggttctc cctgcctgct tggagagcta taactgccac agactgagtg gtttatacca 77100catgcagatg ctttgctgcc tacatctcca gaagggtcaa agggctgttt tagaacagcc 77160caactcactc taaaaaaatg ggctttatga gaagcgatgg tgcagatcat ctggataaac 77220tcacccataa attaatagaa acaggttaat tttccttctt tactcaggtt tccacagcac 77280aaggaaaagc cttgaaatgt tcactagaca agagagggca cgcaactctt tggttgcgtg 77340cttgggtgtt tcctctgtac cctggtctct gctgctagga ttgtttatgt tcttaaacaa 77400tggctgtata taataggaag ggtggagtat tcttcagatt ttgttttggt aatggggatg 77460cttcactatc aacatatttg ctcctggctt tggcagcggt gttcaaaaat ttgctgagaa 77520gtttatgtaa ctacacattg gcataacaaa tagcttgcca ctgtatggcc aatgtacatc 77580cattcctgtt caagcaggaa taatcagcct agaaagaagc aggaaagata catcctggag 77640gtaccgatgc aaaataatag aaccagtcag gaaaagccct ttcctgtata aaaacagcat 77700catgaggggc taagttgctt gggagatgga cttggcaaca cttctcctga aaagatattt 77760tgtgctgaaa tgtattgggt tttaatttaa agcacattgc tttggaaatg ctttgtgttg 77820catggggaag actcgaattt ctgcgttaaa ggaaatttca tttttcttat gtgttgtgtc 77880cctttaaacc caaaaagcca cagaaacact ttgaaagttt ttgtatgaat ggtcatgaaa 77940aataacttct acaaccatag gcttttcatg tgaggacact gtattatctg ttgtgttctc 78000cttttctagg atagacacgt atcatttccg ccaattctct cttcctttgc ttatgagaaa 78060taaatgtata ttaaagcact taaatgagaa gaagagtaag tatgcaattg gaattatcat 78120gcagcatcag ggaaaacagg tttcttcttg ctttcccttt ctacatatag aactgcctta 78180caaaccaggc taccactctt tcagaatctg cattttattt actgcctcct cctgttgact 78240gcataatgta acataccaca tcttttaatt atgatagctt tgagcgcagc ttttcattct 78300tcagtaagtt ttgccttgat ttcatcttta gcctaaaaca agctctacag agagaacaga 78360gcgtgaacag ctatagaaaa ggagtatttt tcacttcacg gagccatgga agcaatttgt 78420tatccttaca agacttctgg tatacagtgg tatctacgga aggaggctct tttcctgggt 78480agatccctgc tcacatataa caacgccagc aattccacct cccagactgt taacagctac 78540tgagccatca tgcaaagcat ctcccttcat cagccataaa accacagccc tgcttgctgc 78600ctctgcacaa ttgctaatgt tctgtgcaaa cagtttgtct ggtgcagaac aacagctgtg 78660atctttctgg aacacttctt ttgatcttgt attttctctc cttcccactc caagatcttt 78720taaaagaacc atttccattt gttgcccaac atctaggtgt tctcaaagtt actctgccct 78780cacggtggct ccaaaactca ccaacaaatg attacagaga tcataagcat ggcttaatga 78840tgtggaatca tacctacaca tactccctct ccaaatatcc attaagaaag ttcactaaat 78900cctttggttc tctagtaaga aagttccttc tccagcccac atcccttctc cctccactgt 78960tgcattgctt ttctggggca gccctgtaaa tagctcacat gaagccatgg aattggtggc 79020agtggttgta cctggacgtc actctgaaga cagtctgctg ctttttctaa aggcatggac 79080acctctgtac gccagacgct tgcctttaag acctgtttcc agctctcatg ctctccctct 79140gtgcttggtg gttggttctt tccctgtggg ttggggtgga ggtgcctctc ttctgttgag 79200gaagttcatt agctcctgtt gtctcctcga cgccttctga ggtctagaca cacctacaac 79260atgcatcctg acctacattc acagtaaaca acctcttaga tccattttag atcttttacc 79320agctgtgaaa gtggagcaac acaaacttta acatgaaaga agtgctgagt tttgttttca 79380gaaggttgtg aataatagct aacgagggtg gaagaaaaga gaaatgatta ctgcaatgtg 79440tttttcttgt ggtaggatga ctgcccattt atgttaggcc ttcatatgaa gtactactgg 79500acttcagggt gaaacaagtg tcttagaatg aaacatatat gaacttttta tttcaagtta 79560ggtaaaagga aataaatgcc tgcacttgcc acatatcagc accttcatat gttcagcaac 79620ttgactttcc tgtcaatcta tcttaggcta agcctttttt cttgtgggct gagttcattc 79680ccattgtctg ggacttgctg caagctaagc tgctcgcaca gacaacttgc tgcacctcag 79740cagagccata gcaacttctt acaccctgtt aactttggtg cctgagcccc cacttgtcat 79800acaaagatcc tgcctgtctc acacctgaat gagaggcagt gtgtgttccg catccttgca 79860gtcagtgcag gacgctgagt agttcttgtc ccagagcagg ctgaaagcta gagccaccct 79920gacctgagtg ctttctctcc acactgtgct atatattttc ccctaaataa aatatctttc 79980tggaacacag gccacagtta cttatgtctg caagcagcca agagcatatg ctttgctttt 80040cttacatatt tctggtgtgc tgtccagaac atcctttgtt tgacactaaa attgatgtgt 80100gctttttatg gtacaatatt ttgagaaaaa cttgagtact ccactgctat ccacacaaca 80160gctttacagt tatttcccta aaggactgat aagggcttct taaaagcctt tttttttttt 80220ttcagatggc attcttcatg aaaagaccaa gctgaaactt agtcccaaat tcttcttacc 80280agagtggatt taatggccca taggaaaggc atcagactgc tgtatttaca gtacaagaga 80340aaagaatgag acagatcttg tcctgccatt gaacaggaag cttacagact ttctggggct 80400gctgagctat tgcttcgttg tgaaattgcc attcgttatc cattctgaat cagtggttcc 80460tatcaaatca atgaggagac atgaagtata ctgcaaacag tgcatgtttc cataggtagt 80520agcattcata gctgcttacg ttccttcttc atacatgaaa ataattacta gtaattttac 80580tttcatgaat ctgttgtttg aatccttcac actgcagctc aggttaccag atgtggttag 80640atgcccgtgt agtttctgtc accccaatct gtctctaatc atgttgttac aagaggaaag 80700aactgatgcg atgacacaca ttaaactagt ttgtagaagg aaatccacgg ctgactgatt 80760taaataccac aaccttttgc ttacaaataa gaacaagaca gacagaccac gggaaactct 80820tttggaaggg atcagataca ttgtgggata agatggaaaa acaattctct ctaaggaatt 80880ctcatatggt atgagtattg gggccccttt ccagatcctg ctgtattcac atgagtgtga 80940attaatagat gtgtgcaaaa tcagctattt caaactcaga attcagcaca cttctactat 81000ttagcaaccg actatgggat gattttaggg cggacagata cttcacagta tgatacagat 81060aagcaatcag ctgattcaca tttctccttt ccctttttgc tcccagtaag ctgcaggctt 81120cacaatgggc tccatttcta gaatgattat tgagttttgc cttgatctct acaataaact 81180caacagaaca gcaaaaggcc aaaacattgt cttctctcca atgagcatct ctacctccct 81240tggcctgatc cttctagggg cacgaaacaa cactgctgct cagatagaag aagtaagtac 81300tgctgaaatg ttctgagata cttccacata gcctgctgtt cccccagtgg caatgctggg 81360ctttgcagca caacatgtgt gcttaggaga caaagataaa cacaagctca actgctgcct 81420tgagagcagt gcttggtgtg ctgtgatccc tgctcactta tcaactgtga cattcaaacg 81480attcaacatg tctcacctac agagcacacg gagcctgggg gtacagggtg ggcatgcaga 81540agtctgttcc tctggtcacc atgcctttta ctccctgcag tgcaagctgt atgctctgag 81600atcttttatt tcttttctta tttgtttctg agagcagtaa gtgaccaata ctcctaaggt 81660atatgtggca taaggcagta gctggctctg gctgtgtcct ggtggatctt catccattgt 81720attataatat tgccacaggt cagctgctgc caagggaaac tcattctcct tatgaggttc 81780tgagtgactc ttgcttagtt taggaaagca atggagatcg agtactctca acaaggggga 81840atggcgtcta actaaaagag cggaaattta ggtaagatgt taggcatata ttctttacac 81900agagggcaat gaggcaccag cacaggcttc ccagagaagc tgtggtgcgc catccctgga 81960ggcgctcaaa gccaggttgg atggggccct gggcaacctg acctggtggt ggcatccctg 82020cccacagcat ggggttgggg ctgagtgggc tttgaggtcc cttccaaccc aaacctttct 82080atgacagtta ataaatctac atcacttatc caggacagcc cagtaaatct ttcaaacaag 82140gaaaatgcct ttatcccagt taaaattgcc attaatttga cctcttcaac tgcaggttct 82200ccacgtcagc aatgccgcag gaactacaag ccttgaatct gagcttgaag gtgcagtgcc 82260cgaaaacaag tctgaactaa gccaggaaag agagtcttcc ccctctctgg tatgtctttt 82320ttagtacaag agtctttcac tccacagtag cctattagtt gtaaagcacc acagcctgcc 82380acaggaggga gtcaagatcc catgcacaac gtctgcctgg tctactacgc ctgattgaag 82440gtgttccctt gtaatcagcc aagtcctcca taaagtcaaa tacaaagccc ccaccagaag 82500gaagatcagg ttacaaaact tagattagct gaatttaaat ataattacag tgggagctag 82560ccctacactg caatctaatg aggatgcaaa tgaacaacca aagctatact gaggaatact 82620tgtaattggt gtgtttgaaa tattcctagt gcaacacaga tgggaatctt aaccacgaag 82680cgttccatgc actgctttta caactacaaa accttggcaa agactatgtt ttaagcctgg 82740ctaacagcct ctttatccaa caaggatttg aaccgcatca ggtaagataa ctgtaccttg 82800taacctctgt ggcgctgacc cccagctttc tggcaaccat atgcttcact gttgtccctc 82860catgtgtatt tttgagcatt ggaggtgctt cttggagcca tatctcttag ggttgttggg 82920aaagagacag aagtatcagc tttcagtgct tctgtttaaa acaaacaaac aaacaaagtc 82980aagacaacac tctgtagagc aaaaataaag cagaagacct ttgacttttg gcatatctaa 83040cttgagccag aagtgcgact acagcaaaaa aatggcctat tcaagctgtc tgcaagctgc 83100ttctgggcta tctttctatt tgcagctttg cattgctggc tttcctcttt ttcttctttc 83160tttctttttt tttttttttc cccctgctga atgatttgga tacttgagaa tcacccaaca 83220catcttgcat cttctctaat ttttttttct tttctatttt tttaaatttt tatctggata 83280cctgcatact tcaggtatgc agttttctgt gggaagacat tgtcatctag aggcaaaaat 83340gtatataaat aataagaaag acacaataat aatctctttt tcaaagatta tctgaatcag 83400cttctgatag ttgatgtttc caaagccaaa ttttgtctct ttcagtcaag aagaccctca 83460gaatttctaa aacgtttctg aattgttgac ttcatgttaa agagaataag ctctgaacag 83520gtttggctaa ttcacaatct ttattctgct ttacagaaat atctaatgtg cagtaaggaa 83580ctatacagag cagcccttga aacagtggac ttccaaaggg ctcttgaagc aagcaggcta 83640aaaattaatg attgggttga aagcgagaca caaggtaaaa cagagcaaaa ctgtagctgt 83700gctatcttct ccctcttcca gtgctccttc aaaaagaatt cagcatatga taagtcttgt 83760tcatgtttct aggtttctca tgcccgtcaa agatagtttg ttgttcccaa tcattcttta 83820gagtcatcta ccagctaaac tatttctgag ttaaagatgt gtttgttgtc acatactgtc 83880atactcctac ccacatgcct agcaagataa ctgcaacagt acctctaagg gttaaataga 83940ttaattgctc ctgcaaatag ccaacactgc aggtacagta aagcagagga cggaagttat 84000gagcgtcaca gtgagactgg gaacagcata gcagagagag aagacacctg aggacctggt 84060gttgacctgc tctggtcgta cacagagcaa tgctaacaaa gatgagtgat gtgcccacca 84120gagagatttc actgttacaa gtaacaacca accagctttt gccctttaca ggcacataga 84180ggtcattggc tttttttctg attaagctga acatgaaata tgccactttt attttgtcag 84240agatgcaaca tcagcagggt gaaaacctta taaatcttcc agctgaactt aagccagaac 84300ttactgaggg aaattactga tggatgaata gatttgaagg cttctgattt cttaatggtc 84360atatcctgac caaacctgtc cttgggctga cagagcagcc tgtgactaat gtgggaaaga 84420gctgcaaacc ccagaccatc attgctctgt gtgcctgtac aaagcctgcg cgcttgggaa 84480atcctacttc acctctgtac agaaaaaaaa agggtaaagg gaaagatgcc ctcatgtaaa 84540ctgaaacaga ggattaatgg cgctgcgcct tttactgtgg acaggtgcca cctggaacat 84600tcattttgcc actgatccca cagtaggcta atttgatgat cggtgcccct tcctctccct 84660aacaggccag tactaggtaa cagtgctgag aaatttacca tttctttgct tgtatcgtcc 84720ctgttctgtg aagaaacaaa cagttggatt tctaaggtac tctaaagcta agttcacaga 84780caagtaattg agtctcaatc cagagcctta ataacaacta ataaacacct gtgttttcca 84840aaatttcctc caggtaaaat caaggaactt tttgctccag gagtgattga ctcacacacc 84900attctggtgc tggtgaacgt gatctacttc aaagcatcct gggaacacaa gtttgaggag 84960aaaaatacag tacagagaga ttttaaactg aatcaggtag atatgcattg tataaatctt 85020agcatgattt acctgagtta gcatgattta catgagttgc aacgactcag cattttgttt 85080caatggctga caaaacacaa agcttcagcc ctgatcagcg cttttgaacc taatagtcac 85140tatgggcagc tgtcatggat agaagccaat tgcaaagatc tcatttcaca caggctctgt 85200ggggccatcc tggcttttat gcatcccgta caattcagcg tgagccatgc aacagatagg 85260ttaaaccaaa ccaatcaaaa aaagaggcca gatattaaca agccacatat atgaagatgg 85320aatttgaaac aggaaaaatc ctcacagagt gttttggttt atttatagta tctgcaatgt 85380ttaaaaggtt ttttttaaaa tatttttttt attttgattc cttttttcca ccgtacatat 85440aaaatggaag ttttcattgc tcaactaagg tacagaatca tagaattact caggttggaa 85500aggacctcaa agatcatcaa gtccaaccgc agcctaacca tagtacccta actctaacaa 85560ccatctgtta aatcatatct ctgagcacca catccaaacg gctcttaaac acatccaggg 85620atggtaactc aaccacctcc ctggggagcc tatcccagcg cttaacaacc ctttctgtaa 85680agaagtgttt cctaacgtcc aacctaaact taccttggca caacttgagg ccatttcccc 85740tcgtcttgtc acctgttgcc agtgagaaga gacctacccc gctctcactg taagcacctt 85800tcaggtactg gaagaaaata ataaggtctt ctctcagcct cctcttctcc agactaaaaa 85860gccccagctc cctcagcttc tcctcgtagg actgattttc caagcccttc actagccttg 85920tgaagctgca aaaagttctt taacaaccac attaatccaa gctctgtaca gctcaagtct 85980aacaaatgtc ttcaaaaaag atgatcaaaa ccattttatt tcatttaatt cagttttgtc 86040ttcattccat atgctgtgcc tatgttacac taaataatga agccgccaaa aaaatgaacc 86100cacaaaaaac acagatttag ctctgatctg aagttgaaga gctttgtatg ggaaaaactg 86160tattctaagt gtttcttatc tatacaaaca aaaggtcaga aagacatctg ttgctagccg 86220tagtgttgca ctgccattta ttaagacacg taagaaagtg taattttggt cccttaattt 86280ttttacttga aatatgtctt tgaatttgaa tactgaaaac tgaccttagg taggaacatt 86340tggaacactg ctgcagtcac agaaactatg agattggggg aatctgcata tacttttctt 86400catgcactaa ttaataatgt tctctactaa aattcttccg ctgatttaga aggtaagtaa 86460aaacttagct aatggtgaaa tgaaccttga gcctttacac aggatttgaa caaactcatc 86520acaaaagaaa atgaggctta gaagacctag aagaacatgc ctgagattgc tcttaatctg 86580tctattgctt cctgcctaaa acatctacct gataaatgac aacctgattc ctgcagtgct 86640atttcttctc tatcccattc caaaccagga cttgcaaatc ccatcagcat cagcttgttt 86700ggctggagag taatggtatt aagccacttc actatctgat cagttgcagg gaaattgctt 86760tgttttattt tgccccccag agaattatct cctttataca tgaatggcaa aactgatgtt 86820ttacgtgtcg ttgtatgtgc aacaaaataa agaaaaaatg tttagcttta taacaattac 86880tgctgcaaac acagactact gatattgcac ctgaagttta aacattaagg tctgtattgc 86940ttgtgtgatc attccaattt ctttttaaat agaatgagag aaagccagta cagatgatgt 87000atcagaaagg cacatttaaa ctaggctata ttgaagagct gggaactcag gtgcttgaac 87060tcccttacgc tcagaagttg cttagcatga tcatcctgca ccaggagaga cagcagatgg 87120atctcccagt ggggctggaa caggtaaggg tgaggactgc ggctaagccg gactgaaagc 87180tggttgtctg aattaaagct gggcaaaaat ctaaacttgt taatttcccc atcttctaga 87240ctgaaagcac aatgacctat gaaaatttaa tgctgtggtt ctcttccgaa catatgtttg 87300agatggtggt agaggtgtac ctgccccgat tcaagctcga aggcaccttt gacctcaatg 87360aggtattaaa agcaatggga atgactgaca tcttcagtga atccaaagct gatctttctg 87420cattgtcatc tgagaaatcc ctggtgttgt caaacattgt ccacaaggct tatgtggaag 87480tcaatgagga gggtactaca gcagcagctg ctacaggagc taccattgtg aggaggtctc 87540ttcccctcat agaggtgttc atagctgacc gtcctttctt attctttatt aggcacaatc 87600ccaccagtac cattcttttc tttggtaaat tctgctcacc ttaaaatcaa ggccatcttc 87660tagcattgtg agaaaaacct ggatgaatca gaaatactat ttttccccct acaccttctt 87720attcctatga atgattgtag atcaaagtaa tcactgcagc caacctagcc tagaaccatc 87780aattgaatgc cctcctgtta tgctccttga atggcaaata ttgatctgaa tctaaaacag 87840gagtaagttt tcccttaacc tgactggaaa tcaagaatat tttgtttctt caaggcgtac 87900atacactcct gtatagccaa gtatgtccgg catagccaag taatgtagta cactatttgc 87960ctggcaaagg tagaatttgt atgctgctac ctgaggagaa ctgtttgtaa caattttcag 88020taactgccag taaaagtgga gtatttttat tttctctgta gtttttgatt tcctgccagg 88080tgggacttga ttaacagaga ggggctttgg aaatgcttta tacttataca taatctgtat 88140ttgtggcaaa tccttcgcac agtggagatc tcactttgat aattcccttt cctgtagcag 88200cagtcacaag caagcaggaa atacttattt acagcaaatt cacgtgttta ctgacaactg 88260taccaccttt ccccccatga tgtatgctgg atctatcctt ttgccatata aaacgtttat 88320gctagaagca gctttggttt catttattta tttagatata agcctgcatc tgaagcacca 88380actcatcaac tggaagatag atggaatatg acatataccc ctttcacaat cccttggttt 88440tttccacatg agttctgtta gaagcactgt atttttcctt ttttaagata acaacagtag 88500gaacactcat ggaaaggaca agattacgcc tcatgaacac atctagtaag agagttgatt 88560ataacagcaa ctgagtatgt gggaaggcaa gattttgacc ctcgttttac aggatttttt 88620ggcactcttt tttgaaaata aatccaccct taaagaatca cagcatggtt gatgttgcaa 88680gggacctctg gaggacattt tgtccaactg tcctgttcag gcagggcaac catgtccagg 88740gggcttttga gaatccccaa gcacagaaac ttcacaacct ctctggacaa cctcttctga 88800gttcccacaa ttttgaatga caccaaagag aattttgtat gcgcagtgtc tgcaggaatg 88860ggatgtgaaa acacacattt ctaaagctta attacttaca tagtgaagta attggttttc 88920ttccttgagt tctgctctct ggtgaagttt aatgatctga gatgcatgta tatagatata 88980caggtctctc cagccctgag gaatgaagaa aagttttgaa aagggcaatg taagcaatag 89040aaatcacagt caaatattac ctggaaaact ttttagtctg agagataatt agaaaaatag 89100aattagcagc tgactgatag agagacataa ctgttaagtt gctggtttaa cacaagtaat 89160atcttcctca cagagttcta tgtgaggttt aactaactag cgttggcaac ttgtgctttg 89220tgacctataa aaaggcaagt atacattagc tattagtcat ataattgagt gtaaagctcc 89280ataaagtaat tcatgattag cacagtttat gtaccaaaag ttacctgcgg ctctttggat 89340aagaaagtct aggcatgatg ttcgagcaag aacaggcagg agtaggacaa taatattcaa 89400acaacttacc cttactgact aatctgaaag cacagtacaa tgtaagcagt acttttccag 89460attgtgtcca tgtttccatt ctggaggctg acagcacaga ttgcctacta agctatgttt 89520ttattacctc caggtgtcat cacttggttt ttacataccc tggggaagtt ctgagcacca 89580caacctcaaa catcagtccc acttctgcaa cgacaggaac agagattcct gtgatgaagc 89640gtcgaataac acagtgtctt gctccagttg ttggaggaga tggttcatga taaatctaga 89700gtgagattaa gacacagatg aggtcaaatg tcatccagct agtttatgac aaattctaag 89760cagttaagga atgtgggaaa catggcaaag ttagcaacag taaagggagg aattctagca 89820aactggctat agagcaggga tactcacccc catggatcta gcagtatccc attggtttgc 89880aggaggttgc aggtcagtca aagacatatc actgatctgc acagctgcag ttcagtggag 89940gattgtctct gttctaccac tgaactcttc aggctttatc ctcttcattc tgctctcatg 90000caccttcagt tactcagggc caatggcatg tgtgcctccc attgggtgat cggctgttga 90060tcatgcagca atcacacacc tgccacctgg cacgctgttc ggcatgtgta ctgacttaat 90120ggaagagacc ttttaagctc atctagtcca actcccctcc actgaagagg gacacctaca 90180gctagatcag gttattcaga gccccgtcca gcctcctcaa tgtctccagg gaaggggctt 90240ctaccatatc tctaagcagc acattccagt gccccaccat cctcactgta aaagaatttt 90300tctttatatc caagccaaat ctcccttcct ttagtttgaa

actatttccc cttgtcccat 90360tacaacagat cctactaaag aatctgtctc cttcttctta agagctccct tgagaaggga 90420gctcttctca ggtcaccttg gagccttctc atatccagac tgagcagtgc tagttctcag 90480cccgtccttg taggggaagc attccatccc ttggattatt ttcctctgga ctcacttcaa 90540cgtccatgtc tcctctgtac tgaggactgc acatttggat gtagtactct aggagaggcc 90600tcaccagcat agagcaaggg acaggatcac ctgccttgcc ctgctggcca tgcttctttt 90660gctgcaacct aagatacggt tgactttcta ggctgcaagg gcacactact gactcacgtc 90720cagatgccat ctaccacagt acccctaaat ccttttctgg cagggctatg ctccctcttt 90780tcgtattcca gcttgtaaat gtagtggggg ttgccataac ccaggtgcaa gaccttacct 90840ttggatttgt tgaccctcat gaagttctct cgggcccact gcttgagcct gtatggatcc 90900ctctgaatgg catctcatcc ttcaggagca tccactacac catacagctt ggtgtccttt 90960gcaaacttgc tgagggtgca tcaaaatcct gttgacaatg ttactgatga agacactaaa 91020gagtactgat cccagtactg atccctaagg aacactactg gtcactgatc tccatccaga 91080cattgagcca ttgaccacca ctctctgggt ttgatcccgc agccagtttc tagtccacta 91140gtcagcacac cactgatcat agccacactc gaaggggcag tcatgcaagc accaccctgg 91200gtatttattt cccagcactc taaagcagag ctcttgctcc agctcatgtt attttctgtg 91260tggcaaggag tgagattcat cgactctagc aaatggaact aatggctcca tgtgccccag 91320gtctcagctc agcaccagcc aggccagggc tgagtccccc cacatccaac ccataaggtc 91380ccagaggact cctacgttta ccagtggtgc acagagatga gtttagccca agtccacccc 91440tcagcctcaa ctcccttcaa cacctcttca ccaagaggcc caatccatca ctccttacca 91500gccaaaacat atacttgttt aataccacag ccacaaaagc cacgtggtaa ggtctgaaga 91560gaccaaaact gtggtttgag taaaacagaa ggaaagcctc tactcagtac cccacttatg 91620actgagttac taggatagga cctgattcta cagcacccca ataccctgta gatgtattcc 91680tttaattctt cacaccagat taaggctgct gccaccaccc accacaaata aatccttgct 91740taggctgatt ataacttaca cctgtggctt ccacagtcaa atgagattcc cagtgcccac 91800ctgcgtgttc aacttcctta aggcaaagca tcttgcagtt agcagagtgt taagaaatct 91860tcttgtattt cctttaacac acgtttatct tccccagtga tgctgaattt gcaaatgctt 91920tagggaaaaa ttggcagcaa gtccttacat aattactgtt tagcctagaa aataacaacc 91980gaggtagaat acttcagaaa gtttctaatt taaggttttt ttcttgatga gagaaaagtg 92040ctatcagagc tgtttagtaa ttccagtcat gcatgggtaa ctcattcttc tgtgttaggg 92100tttactgaga ggtgaagaaa caagtagttt cttttcctta tgaaaaaaaa aaaaagtggt 92160attagaagaa ccccataaaa gaatgccaaa cattgcagct tatgatgtgc aatgtgtcac 92220tcagtcttac agatgacaca gcctggaagt aagcttaaaa aaaatgttta attcctaact 92280tcttttgaca ccatctgtgc tgtggtttat gacatccatt aataatgttt atcactaaac 92340aacaacagat agagagacca gaaactaagg atgctgctgt catttccttc tgatgcaagg 92400tagaaacatc aggaaattaa ggcacactga aatattttgt aatattttgg actagaagca 92460aaaccagaaa ctgagttgca tttgtctcct ggagtacatt ctacaggtat ttaaaaagag 92520acaaaaacca taaatctact tgaatttaat ttgaagtatc aaatgaaaaa gatgtacctg 92580attttattat cctccacact ggtcttctga acttgaccaa tcccactggt cagttactgg 92640tttacgactg ctcaagctgt ttgtagcaac tatgttgtac cacaaaatat ctgagccatt 92700acaaaacaga agagtcatta ggcattttat ctccaaccca aagcatacat gcatgtttta 92760aaatctcaaa ttctcctgac tttaattgtg catattatgt tcaccaaacc ttttagaacc 92820tgccttgttt tttttgttct ggtctgtagc tgggagtcag agaaattcaa ctgtgattgg 92880aaaaatggtt actggcaagc tatagagttt ctaagccaga aggtgaagaa atactacttt 92940tttaacactc ttggcctggg actagactta cagacatgat caatattgaa aggcaatttg 93000gaggtataca ttttaacatg tcctcagtct ggagttagct gtgtgtccag tttcctctca 93060gtgtgagtca agcaatagca ttagaaagtt atgccccaag tctcatcccc tcctattgaa 93120acttggcaca gcacattcag gctgtaagcc accaggtcac agccccctta aaggattcgg 93180caacagctgt ggttgctatc acatggtgtt gatcatcgtt gggcccctca ctgtaagaaa 93240gacattgaga ccctggagcg tgtccagagg agggcaacaa agctgttgag gggtctggag 93300cacaggcctt atgaggaacg gctgaaggaa ctgggattgt tcagtctaaa gaagaggagg 93360ctcaggggag accttattgc tctctataac tacctgaagg gaggttgtag tgagctgggg 93420gtcggcctct tctctcgtgt gactagtgat aggactagag ggaatggctt caagctgcgt 93480cagggaaggt tcaggctgga tgttaggaaa tactacttct ctgaaagggt ggtcaggcac 93540tggaaagggc tgcccagaga ggtggtggag tcactgaccc tgaaggtgtt caaagagtgt 93600ttggatgttg tgttgaggga catggtttag tgagaaccat tggtgaaggg cgaacgaatg 93660gttggactgg atgatcttct gggtcttttc ctaccttagt gattccatga ttctatgatc 93720attacactgg atttgatact ctgtgagcaa aggcattgaa gtggtacaaa aaattcaaca 93780ttctgcatta aattgtagaa tctggcaagt ggaaatcgtt ttctataggc acagccacgc 93840actcagaatg tgtttgcaat ttgcttgcat ttagtcttct gcaagtaatg actgctttct 93900gtatgcaaat gattgatcca tgtgaaaaaa tctgcttgtg tatctgtgaa tcaaatgcat 93960tgctttataa tgtgcatttt ggatcattta tttgtggaag taagtgtaaa aaacagagcc 94020tgcaattgtg cttctgcagt atacaaggcg ttactcaact ccagctgtac agtcagtcag 94080gccctgagat aatctagact tatactttcc atagttatta taattttgtc tcttactaaa 94140tctttgattc tgcttgtttg ataaagtaac actcattttc tatatagtat tacaatcgct 94200tctagaaggc attacatcac tgaattcata ggctttctga aaaacagatt cagaaatcag 94260attttctaac tgtatttttc catgtatatg tattggagaa ctagtgaaga acgtgtttaa 94320tatacagaac tacagataaa tccagaaagg agaagcaaca ctcaaaataa ggatgtggca 94380atcctaaata ggctgtaagc tggcttgaag catgtccctc caaaaaagcc atctgagaga 94440aaatttctca tttaccatgc atgtgcaagt ttccaaactc tgcaggtatt ttattttctc 94500cttttgcaaa ttcccttgca gatggcattt tgctttgctt gctctgaact gcgttgatgt 94560gagcagtgag gtgcttttct catgctgaaa tacaagaata aagaagattg aagcacaggt 94620ctgtgcagaa catctagtga atgtattcag ggcatgccaa gcacaagcta ttcaaatatt 94680gctccctgaa aatgcagtca gagtggactt catgttttta agtggaagtg gtacataact 94740tctgtagtgg agaaatcgtg tgactcaggg ggtgaagggc ctatcctcag ttaatcccat 94800attcttgttg caatatgggc ctgcatcttc cagcactgtc agactccagg ttttagcata 94860agatcagtgg aaaaaaatat acacaaatat accccttgct tctgaagctc tgccctaatt 94920gggatgattg caaataaatg aaaaaaaaaa aaagggaaat tcaaatactg atgataactc 94980tgcagttcaa caaccaggac acctagtagg tgagttctgg cttccagtcc ctgctgctag 95040gactattctt gttttaatgt ttaagagaaa acaagtattc acacatgggt gagtacccta 95100gcaataatga cagagaacta ttctgctcta tagcattctg atagtatgaa tctcgcctta 95160attccatagt cttctcttag taccacgtcc cccagctcct gttgtctgaa ttaagcaatc 95220actgtgtgac acctacgtcg gagcttagct ccattacact caatgaaatc agttgctggt 95280cttctgtgga aaatatacta ttgcgccctg agcagtgctg agcacagcac ctgtttgcct 95340aatattaatg cagcactcag accacaacca gcctcaagac actcagcaga aggaatatta 95400tgaaaacagt aggtgctgct cctgaagcat aacagcctcc agagatggaa gacaagaaga 95460tgtgctttgg tagtgtgtgg tgctcatttc cttgttcatg aatgatgatg ggaatgactc 95520tggaagacac accagaggcc tctggtgtat accccatgcc tccagcctgg gcaactcctc 95580cttgctgcct ttttgacttg ttttgtgcaa gccatccatc cagaggtgca gagtgaaaac 95640aaccatggag ctcaagaaga gcctcatcag gtccatacac acttcaaacc cagagcaaaa 95700cattggagcc tcgggctcac tgcacagttc tgctgaaaac tgtgatgaag agctaggggt 95760tagaggaaaa atgtgctgta gttatcagtg cagctccatc atctgttccg ggagcatcaa 95820ggcttcctgg agagaacatt atcagaagga cacaaattat tcagtgagag ggagaagtgc 95880gcctctgaac gctctgagtc agatgcttat tttgtgaatt tttctgtttc cctcttcctg 95940ttatgcttcc tgcagatact tggcacatcc ttgaggcgat tcagcaatat atgctcatat 96000tcagccacat ctacagagtg cctcctccct gagaggagaa aaatatttgt tttagggggt 96060aaaaccagaa tagctgtgct tggacctcct gctctgctgt gggacaagag aagctaggct 96120cctggtaacc tcaggaggca gagggaggca cattataatt tggctaagac ttgaaaatgc 96180aatttgttgg tatatttggt aaatatactg atggcctagt cccataaact accttctaga 96240tgtggagtaa gtggtttaaa ggcatagcta agaggttgca gaaaagaaag gaccacatcc 96300aatttggtag caaccaacat ccagcattca cagactcatg agaaatacct tttaattaat 96360ttatttatat taaataaaaa aaaaaaatcc tttgatgact caccctgctt ttcctgttac 96420tctcagttgg gaagaaagta accgctgggt acatactact gcaatttcag agctgcagac 96480ttgaagagct ttcccaagtg ctgagatatg caggaaaaaa aaccctgtaa attacagtac 96540caggcattta attttgattg ctaaataaag aagactcgtg acagtccatg actacgtctt 96600ggagggctgc aattacatat gaaatatagt ctgaattagg agagttactg gcagaggcaa 96660agtttgcatg ccaattaatt ggtaaaagga gagtacgcca aacacaggct gtggactgct 96720ctgatgaact gagtatgtaa aaaatagcca tgtgtgtttt tcagtgaata ccatggtata 96780tgtctggttt gagtcaaata tgtattaaaa tgaaaaaaaa aaacaacaag aacagtgaaa 96840taaacagtgc tagcatatat tagcttgtat aatcagacct atatagtttt caaataaatc 96900ttcaaggaga acaaaatgta tagtatgtat gataaggata agtactataa aacatcatca 96960tgaggagtgc cagtctgaca acaggaaaag gaattcagcg tgtgaatgaa ggggaaagtg 97020tgactgaaac aattgtcact cagcttacta cagcagaagc aatcatttat gatcttagat 97080ttttttttat tttttttttt aacttgcttc agagatatct aagtaatctc aaaaacagga 97140acaaaatacc aacgcaagga aaaattctat tttcgcttca tataatcttt tctttttttt 97200tctagttgca ttcttaccta aaaacaacaa caacaaaaca tttaaacaat gtttaaatgt 97260ttactgctgg tttgattaca tcaaaccgag ttgttgctgg agatgaccag ctatcaaggt 97320gcataatgga ctggcagatg tgcttggtct taccccaggt tgctgtgcaa acacaataca 97380cattgacata taagctacta tgagttctga agggcagttt agacattaat tctactccag 97440gccagacacg ctgactatct gagtggttta tagcaaggga ctggttgact tcaaagtggt 97500tccaagtcaa ccactgccaa gtgcttaaga ctgtgtatgc acaacagagc tgatcatctc 97560cagtgcaaca aataacatga gagcaaaaag catctgaaat tctgtaaatg aggctgttct 97620ggccacacct tggctcatta aaagactttg agagatgcca gaatagcctc tgctaaatgt 97680gatgcagatg gacaagctat ggaatgaatg ggtccagggc ataaggaaac attaccctca 97740agcactacac aggagctgct gaacaaccac aggaaaggaa atgtgaaaat gtgaacagat 97800aaatgttgga aagagccgca tttctgctgc ttactatgtc cttgattatg ccaacattaa 97860ggaagaatgg caaaccccgt gaattggttt aggaacagct ctacaatgga ctgcctgacg 97920gaggaaaagg gcagcagagt ccttgctgac ctctttctgg tacaaacaca gatctggaac 97980agagtttaac caattagtct tgcttgcatt catgcctctt gaatttcaag aggtgccttt 98040gatttcccct ggcctaacac cccatctaaa attacaaaac catattttgt ctgctgagga 98100ctgtgcacgg atagcccgtt ctggtcaaca tactcaggct gcttctgcaa caagttttgc 98160actggcattc agtgtagaaa aaatgcaaga cctgtgtagc ggcagacttc tctctggaga 98220acatgtattg cctcaactat cttacctgtg caaaactgtt gtggtgactg tgctattgca 98280gaggtagagt gttcaaagaa ggcaaacgta ctgaatgaga gaacacatca aaaacacctt 98340catgccctct tctaggggag acagcgaaac aaaatgttta ttgagaaaat cttggacatc 98400agtccaagag atgaaaacac tgtccatatg tgcagggctg gttgtgttct acaggtccat 98460gctgcataga tgaccacaga ggacaaagac attgaaacca agcatacaaa gggctgtggg 98520tacccaggaa agttcttcaa ggaagccttg aagggatgtt tgagtaccca cctgacctgt 98580agctgcaacc ctgatgtaaa catgtgaaaa tgggagcata agagaagaca ctacacactg 98640caacaaaacc tgtgcccttg gggaggaaaa gtttgacaag ataaagtaga agctattgaa 98700aaaggaacat taaacaagac aggaggaaag cttcttacta tctgtagatt tccctactcc 98760cgacatgact actgtcatgt tgacagataa aaaatactca ttttgagtgt ggaaactgaa 98820agccattcca gttatcatgg tctgcacata cacacatgac tgaatttcag caacacaaaa 98880cacagtgctt atgataaagg agctcccttt tacctttacc agtgggtacc accaccactg 98940tgtactgtct gtcttaatgt gcaaaaattt gggatttcta ttattcattc ccctggcctt 99000aacagaagct ggattttttt ctttagtgct catcaagggc attattcaat aaagagtaat 99060agctttttac aattgactaa tatttgatat tgtgcattat gattgtctaa cagaccatga 99120atgttccttc agacagattt ggtagtttat ttacctgtca tagtaaaata ggaggtacag 99180aagatctatg agaatagcct gtgcatgtac aatgggcctt gttgccatga cctatgaaga 99240atgaaaatca aaagctgacc accaatcatc ccttgaattc cactggctgt tcagcattca 99300cttctgaata tctgaatact ctggagtctg ccttcgcaaa gcagcaaata ctttcagact 99360gttccctaaa tctcttcctc ttacctattc acactgagtt ctctaattca tcccaacacc 99420tctgctctga attttttcat aagaagcttc agcaaaatgt gctttctcct ctcaaatgta 99480tgctgcagag cctttggctt acagtggata tagcccaaat tccagtgaaa aacttcagtc 99540ttgcctaggt gcagaaatag atggagctgt gcttttaaca agtactaact ataagcttct 99600tcagttctca aactctttca gcagaccaaa acatttttca gtacagtttt gttctttaaa 99660aaactcataa agctttgttt ctattcttac atggaaagca atccattaca aaatcctcaa 99720aatagaatga ccatcctgca gctgactctg cttggaactg cattattttc tctacatcaa 99780gtggttgcca tccatgagaa gcatccctat gtttctctgc acactgcagt aagagatcac 99840gtatatatca cacttttccc ttcacccatc ttgggagcag tgctacagta aattgtataa 99900ttacagtgcc ccagagatga gaagaaactg aacagcagga aaggagacac agtcttaaaa 99960agaagaatgt tttccaggaa ttgatgcact ttcttgcact ccttggtaat atgggactac 100020tcttgcctca cctttagcag tgggtgctca ttaaatggtg aatggtggtg ggtcttctgg 100080ttctccaatc atgtcttatt ttctcataat attttgggat ccttagattc atctgactgt 100140gagaatcact tgatctgatt ttttttttta atctgatttt gcagctaagt ttatctgaag 100200tgtattatgc ttatcctctt ttttaagggt tttttttttt tttaaagtgt gtgtattcat 100260tattcgtttg gctctagtta tcgatatggc tcaatcaaat taatgtttaa attctgaagt 100320agagcatgag acatgctaga cttgaagttg gtacagcttt ataagataca agaaaagcct 100380gaataattac attctactat taggtttcac ttcacaaaat aaatttggct ttctccaagt 100440agagtaccag tctaatgttg gcctactcag tgctttcaag cacaatgaat caaaaggcaa 100500tgacaaaggg tagtaactca aaggatgact cttagaaggc taacaggggg agtgtccgaa 100560agggtactgt atatatcacc aaggactcag agaatctgtt caggttcaac tggcaagctg 100620gattattacg agcctctttg atgtttttct gtaagtactt ctccaaataa aatgtaactt 100680ctaagttgta ttcttgaata tggaaaaaac aaaacaaaac agaaatatat tatgtaagaa 100740cttagaggaa aaaagggccg ccttctattt tatgatgttg gcccaccaca tcagaggcag 100800atggtggtgg tatggcagta gaggttgaac cttcccacca acaccccgtt atgtgttgtt 100860gctgtgtgac agatggcagc agaggggcag tctgacagaa tggcgtctca catggaagtg 100920tgtatgaagc aaaggtgtgt cactgaattc ctccatatgg aaaaaaatgg cacccactga 100980cattcatcga tgcttgctga atgtttatgg agacaaaaca gtggatgtga gcacagcgag 101040gcagtgagtg gtgtgtttca gcagtggcga cagtgacagt tgttcacctc cactggtaca 101100gaattttgcc agcaggaaat gcagattctt gtacattgtg ggcaaaaatg catagctagc 101160tgtggtggct atgttgaaaa ataatgttcc gtggctgaga atctgctcaa ggaaataaag 101220ttattgtaat cattataata atattataca tgtgctttct atctattgta gtttacatga 101280aaataaatag gaggcattac ttttggtgtg atctgtatac aggacagata tgtaaaaaat 101340atttctggaa gagaaaattt ttgttttcac agtctcactc cctgcagaac acaggtgagg 101400tacagtagga taattcacag agccttgtta gcaccaggaa cctctcaggt tatgtagtag 101460atcacatttg ctacaaacta tggatatgct attattccaa cttaaaactg ttttagaacg 101520gggagggcac tattcagctt tcttgttctc ggattaaaga aagagaagga ctgtagattt 101580caataatttc ccctaagtct tgacattaaa ttgcatgtac aagaccttca cctggctgat 101640ctgatgcagc tttacagtgc attaagtaat ttagccagac tgtgtattta cggtatatag 101700acgtttgttt gtttttgtca acaacaaaaa aaaggaatca gcagagatta aatgtcaaaa 101760aatgagaata tagagaagaa gcccactaaa gctatagttt ggcatctaag caactggcta 101820gatttacaaa gagattcact ctataaatta caggacagca acctccaatt ttatggccag 101880ttgtacaaag aagcagtttg aaacaagcta agactattgt ggtttgacta catttgattg 101940aaatatccag agtatggtcc agagtagaca cagaagaaat gaaatgtgtt tacattgtct 102000caaaaatcat tcagagttct ctggatggct atagggaaac tcatctagtc cacactgtat 102060tcatcatact gaagcacaga tgaaactatc tatttcctaa agggcaagta caagatagtg 102120tttttataat gaaccagtac ctttctgaag gaaagtaaac atgcatttgg gaaacaatgg 102180gtcagtcttt acaatatttc taatgatcac agaattttta ggctttacat tattgtttca 102240gcatcacaga aacagcaatg aacaagcagc ttctgggcta caggaagtac tttttactac 102300aagtgccaca cgtcaacacc acacagtaat aatcctgttt cttttagaca acaccgattt 102360caggatgggc tccatcagtg cagcaaatgc agaattttgt tttgatgtat tcaatgagct 102420gaaagtccag cacacaaatg agaacatctt gtattccccc ttgagcatca ttgtagcctt 102480ggccatggtc tatatgggag caagaggcaa cactgagtac cagatggaga aggtaagtta 102540tgcaagtaaa tacaagctca ttttgatcct ggttaacaga acaagttatc catgaagatc 102600tttgagactt tctcccctta aggggccagc tgctgtacat ttgccactgg atttgaactt 102660ggctagcaga aggacattga gccatgaggt ttggatctgg aactaacttt tcacttattg 102720cttttcacta caaagggtaa caacagtttc tactaaggag gagatctcct gcttcagttt 102780atattatctc acaaacctga ctccttccag ataaaatgaa caaattttca tgtataaaag 102840atgaaacact cagaaatcag gagtcacagt tctaagtaca gtatgggtgt agctggtttc 102900tggatggaaa aataagtgaa ctaattggaa gatcctatca aaaaatgttc agagcagcac 102960atgcagtaaa aaaacaaaca aacaaacaaa caaaaaaaaa ccacacaaat ttcaacctcg 103020aatgaaactt ctcagttcag ccattggtta tttcaagccc agaatttgaa cacaaaatcc 103080agagactctc agtgaacttt gcatacttca tttcttcttc tgctacttcc atttgcaggc 103140tcttcacttt gacagcattg caggacttgg aggaagcact cagacaaagg tacagaaacc 103200taaggtacat tatttttctc tcacattcac tttttttttt tttcctgaaa acttaaaact 103260gttctgactg tgcttccaat aggtccagcc ccttcccaaa ccctagctaa tgctctcaac 103320acatgatatg caaatgaaaa actaaaattt gttctaaaaa aaaaaaaaat aatgacaaaa 103380agaaggctca tttcacatgt tgcaccagaa aaagtgatag gatagttgaa ggacattttg 103440agcaccagga taccttccta cattgataag aacttgcaca cttgtagggc ttgctggagg 103500accacacatg aaccatgtgt gcttttctcc ttggtcactt gatacatttg gaaagataac 103560acaagccatg ctcccagggc tgtcctcatc cacttgggtt ctccaagcac aatgtggggc 103620ttgtaaagga caagaagatt tttccgtttc cttttctttt tcctttcccc ctttcacttt 103680ttcctttccc cctcactttc tctctccttt tcccacttcc ctctttcctt tacatttccc 103740actctcctct cctctcctct ccactccact ccactcctct cctctcctct cctctcctct 103800cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct 103860cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct 103920cctctccttt ccattctatt ctttttgcta gagcatttag atggttatgt agaacaattc 103980acaaaacaca atcagacaaa tcactcacat tttctgtttc ttatcaccaa gactgagtgt 104040caccaaatgc tatcagttgt acatgcttat atagaacatc tctcccatgg agcttttaga 104100ctctaatgta ttttgtttgc aaatgtctga acactgtgtg ttttcctacg tgatctgtac 104160tttataaata gttgtctttc tagtaaaata agctaacatt tatacccttt ttcctcctct 104220tcaacaaccc agtgtggcaa atccgtgaac atccacctac tctttaaaga actcctctct 104280gatattactg catcaaaagc caattattca ctccgcattg ccaacagact ctatgcagaa 104340aagtcacgtc ctatcctacc ggtgagttgt acaacagagt gattttttgc tagatcctgt 104400ataaacccat aatccaggag tactgcccag agtatctgtt aatccaactc acctcagcgg 104460tgtggacttc cacagctttt catttgacat tctcaaaata aaacacacaa atattctaaa 104520tcaaatacat tttatcttta aaaatagaga aaaatgcttc aaaaataagg attttattat 104580aacaaaacag ttgctaatgg atgctaatgg acctgaagct gtttttggat tggtatttct 104640tcaagaaaat atttcagcat ttctactacg taatcttatc tggtaaagta ataaaaatct 104700taaagatctt aacatatcat gcatcgaaat aattttgctg gcccagtttt aaccatttcg 104760tccaggaaat aagccatgaa aacagtctaa tagcataatt ataaaaatca tggaacattt 104820taactgcatt ttatttcacc ttcacagtct ttttaaaaac tgacttggta gctacaactg 104880ttgtctttac agatttacct aaagtgtgtg aagaaactgt acagagcagg tctggaaaca 104940gtgaacttca aaacagcatc agaccaagcc aggcagctta ttaactcctg ggtggaaaag 105000cagacagaag gtaagctcag aggagagttt ataatatact tccttgttac tactttaccc 105060aaacaacttc tggaaagact attccttcca tctccattaa tggatatttc ctgtggaaac 105120tgatgactct tgcacacttt tttgtgtgcg gtgacagtga atttaaatat atatgacaaa 105180ggcagggatg ccactgtgtg ctttctgtgt aaggagagca taactcatgc aagattggtc 105240ccagcttccc tacaatattg gcatcatttt acaagcatat gctggatgga taagaaatgg 105300gcttccgtgg aagaaaataa tgtggccact aagttggtgt aagaaaagga atgattaaga 105360gtgtatgtac atttatcagg aaaaaggtgg gaagaaaaca

agaatcaagt attagaagga 105420agcacagtga gaggcagaag atcggtatcc ctgctttgct tttcacttcc ttctgttcca 105480tgcaagtctt tttccaagga cgtttgagat attcctgggg atgtgtgtga acattcaagc 105540ctacatgcct ccttacagaa atgcctggtt aagggttagt tgttctgtat gaaatcactc 105600gtgaacttga attccacatg ccatcattta aagaacagga agtcaactca agcttgctgg 105660ttgacatcta aaacaaaaca ctcctgcaat gaaaacaaaa ccccacaaag cagcaccctc 105720caatcccttt gcctcataca tgcaaaccag acagactgtg tcttagcact cactgctttg 105780cttccttctt acaggacaga tcaaagattt gcttgtatca agctccactg atcttgatac 105840aacgctggtc ctcgttaatg ccatctactt caaagggatg tggaagacag catttaatgc 105900agaagacact cgagaaatgc ccttccatgt aacaaaggta ggggacgtgg tcaccgcttc 105960tgggcaggac agaaagccat caagggtgcg acatacacca tcctacagtc attggtccat 106020ggttcttctg ggcccctcgc tgacagggca tggggctgag cccaagacag gctggcaaaa 106080attgtgtctg accaggcatc caaagcacac ctgtagacaa gagaggaaaa tggagacaca 106140gcttgaggat ccagcccagt tcctctgaag gacttgcaca tctgcctgct tcaagagaaa 106200ctgccccctt ctcacattgt ctcatgcttc tgttttgcag gaagaaagca aacctgtgca 106260aatgatgtgt atgaacaata gctttaatgt ggccacactg cctgcagaga aaatgaagat 106320cctggagctc ccatttgcca gcggagacct gagcatgttg gtgctgttgc ctgatgaggt 106380ttctgacctg gagcgggtac ggccctggca ggggaagcca actagttcgg agttcagtgg 106440gagctggctg ctgttagacc tttggctctg ctctcgctcc ttggctgtgc tgtgctggcc 106500aggcagggga gcacaacagt ggcccaggtg cttccaggcg ctcaggcaga ggttggcctc 106560taaggagagc cctagcctca atgttattaa acaaagagta cagcaaagaa tacaaaggta 106620aaggagcgta gggctgctgt aatgttatag aagggcacgt atgggcaatt cttttcattg 106680agaggcagtt tcatctggcc tcttatataa actcttcagc aaatgttact agaattgatg 106740aggttcaata atccctaata tttttgacaa tattctcatc aaatatttta aataagctgt 106800tctcagaata ccaaagtaga tgcagaaata tttgtgtttg tttggtacta tccactgtat 106860ataaattgtc atggcatttt tttttttgca atctctttca ccagctgacc aatctgctat 106920gtagtgaaat tgctttattg ttctgtatga gacacgaaaa tatttgtaca gaaggggatg 106980tgtcaggtgg aaccaaataa aggagcactg aagaggaaat actagagaaa caaatgttaa 107040aataggaaga tgttgatagg atgcaccttg ggaaactttc tatttttttg taaaataata 107100gtcttgatta aaatgaacga tggaaagaag ttgcattctc atcacaggca ttttattctc 107160tccctctctt ttcagattga gaagacaatt aactttgaaa aactcacaga gtggaccaat 107220cccaatacca tggagaagag gagagtgaaa gtgtacctgc cccaaatgaa gattgaggaa 107280aaatataacc tcacatctgt cttaatggca ttgggaatga ctgacctgtt catcccttca 107340gccaatctga ctggcatttc ttcagcagag agcttgaaga tatcccaggc tgtgcacggg 107400gccttcatgg aactcagtga agatggcatt gagatggcag gctccacagg ggtgatagaa 107460gacatcaagc atttccctga gttagaacag tttagggctg accacccatt cctcttcctg 107520atcaaacaca acccaaccaa caccattgtc tactttggca gatattggtc cccttaaaga 107580gagaaagagc tggcaataac acataccttc ccctcagaaa caaaatcccc ttaccgtagt 107640attatagcat aatcttatct ctttcataga aaagacatac ccgcaggaga ggagacagca 107700cgaagcacac ttactccttc ccttcttgta ttaatttcag aatggcttga tatgagcaaa 107760gactgagcca atgagatggt gagaatgaag acacctatca gccattaagg tgataagtga 107820ttttcaccca aggaataaat agtaagaatg accctaagtc cttgggagcc cgttacatag 107880aaagcaataa gctttgctca tcccattccc tggtaacata ctgctgacaa acccacgtta 107940ccattcctga aacatgggct ttgagatctc cagtctagag gggatgtttg tggaagagtt 108000tctggtgtgc agattattga tttgtgatta tgtcaatttt atttttcttt atttggtaat 108060tgggcaatgg tatacatgtt cactatcagt ggagttgtcc tctcaccata agtcctctca 108120cctagttctg aatttcttgc agaggttttt caaagtcctg aagagtctcc cttccattcc 108180agagaaggga aatagatcca gttttgcata ggtgcagtta tgccttttct cagagtgcag 108240attcaaagcc tgaaccatag agatccagat gattcttatg acccagaact cagtgagatc 108300cactgggcga aagaactgtc taaatttttg tttaaaactt tggaagacac ttcaaatttg 108360agaacacctt tttggtgaaa aatcctgaaa gtgttgtaaa atacttcttc taagaaacaa 108420attagaatcc tatttttttt ctgtcttctc ttcctacgta tagattgtca actgcagatg 108480tggatcctct ggctcaatat tatattctgt tttcattcta atcacccatt attgatttag 108540atacatacag ttgattttgt tttggttaac ataataaaag aaaaccacaa acagttttca 108600tgtaaattat attagctttc tgaaccacac actcttaaaa atatctttac attttaacaa 108660ctgtgagtaa aacgtgattt agcagaaaaa tgtattctta gaatgaataa agcatgcaga 108720tatgaagttt ttcaggcatt tatgacattt ttaggagtac ctgttttcaa gaagaacttc 108780accaaagacc tacaaccaga gtttgattct tctctgtatt tcagatgaca agaagtaccg 108840caatagaata caaagttatt cctatctatt tttctgtgcc attccaacag gcattaaaga 108900tgacctggca attttttctg gtaaatattt caaggaacaa ctattctaac agttttaccc 108960ttttatacag aatcacagat tcataggagc tggaagagac ctcacgggat catctagtcc 109020aaccccatat gatttcatca ttttatatga tgaaataatc tggaattcat ataacttgaa 109080aggcataaga aaggttaaat agatcaacag ctactaggca aagcatttcc ctatgcagag 109140tcttgagagg aggaactctg atgttaacat cgcctatttc cacattagtg ttaccactgc 109200agtgtcaatg ataaaaggtg atctgtagag taaaaacagc tggtgctaca ggtatgacac 109260ccacattttt tgtagattat caggatactc acaatacaga cacagctgtt tttcaatggt 109320aaaaccaaac attttaccaa gtatacttta ttttttgcct ttagaaatgg aagtagtgag 109380aagaacagtt ccaaggtaag agaaatatca gcatctcaag gtttaccatc agtagtttat 109440tcatctttca catctcttat gtccatagaa tcatagaatc atagaggttg gaaaagacct 109500taaagatcat caagtccaac catatcctaa ccatactacc ccaactttaa caaccctctg 109560ctaaatcaga agcagttgat ccactctgct aaatttaaaa gccagttcac ttaaacaata 109620agaaaactag aggaagatta catttgcaag ctactcttct acgattaata gactggaaag 109680tgcataaagt acaaagatat gcctcaatga tctgaaaaac agctgaggtc aaaatggaga 109740aatggggaaa aaaattgaag gtttcttgct tgcaagcaaa tataaagctt cccctttctc 109800aaaaagaaaa acaagagaca ggaaagagtg gaaattcagc aatactgaac aaaaattgca 109860acaaaatact gatggccaag ccctggccac cactgaccag gcagggggca gaacatacaa 109920agggcaggat aaaagtgttc tccatgaagg gggtggcagg cctggtgggt gggatgatga 109980acaaaataaa tacactttta aaaccttcct gtggctccag gcatttttgc cccagcctac 110040caaaggctat tagcattttt attttctgga gtattaagac cctgtttctt tgacagacta 110100ctgtgcagca actgacagaa ggtttagtgg gtaagtcaca ggggataaaa tgttcagcat 110160agaccaaagc aaaaacataa tgtcatgatg ggtgatcaac tggctaacag gttgggctca 110220aaggattaca gttactgggg ttacatcagg ctggtaggca gtcactaatg gggttctgca 110280gggctcaatt ttagggctag ttctcttcag tgttttcatc aatgacttgg ataaaggact 110340tgaagtcata ctaagcaagt tcatggatga cacaaattgg gaagtgccat tgactccctt 110400gagggtaaag aggccttaca gagagattct gaccaatcag agagcttggc aatcatcaac 110460tacataaagt ttaacaagag caggtgtcat attctgcacc tgggatgggg cagccttggc 110520tgtgtgtaca gactggagga caagaggctg agagcagtcc tgcccaaagg gacctggggg 110580ttctggctta cagcaagctg tatctgagcc agcagtgtgc cctggcagct ccaagggcca 110640accgtaccct ggggtgcacc aggcccagca ctgccactgg gtgagaggag gggctgtccc 110700actgtgctct gtgctatgca gctgcacctc cagcactgca tacagggttg gctgccacaa 110760cgtaagaaga acacaaaact attagagagc atccaaagaa gggctatgaa gatggtgaag 110820ggtgtggagg gcaagatgtg tgaggatcag ttgaggtccc tgggtttgct cagcccagag 110880cagaggagct gaggggaggc ctcatgacgg ctgcagctcc tcacaagggg agtggaggga 110940cagtgctgag ctctgctctc tgtgacagca tggggctgtg tcaggggagg gtcaggttag 111000gggttaggaa gagggtgatg aggccctgga acaggctccc cagggcagtg ggcatggccc 111060caagctgccc gagttcaagg aacatttgga aaatgctctc agatgtaggg cttggatttt 111120gggtggtgct gtgtggagcc aggacttgga cttgatgatc cttatgggtc ccttccaact 111180caggatattc tgtgattcta tgacaagatg cactactgtt ctatgtgtga gatactactg 111240ttctgtgtga gatactagta gccaaggcct tcacagggcc tttctgaatg tgcctccagt 111300gaatggtcac cggagtaatc ccctctgtca acactgagat acacatctct gtcaccatct 111360gtgacaggct aaggcagcag tgcaggcaac aatgtcaatc tcttcagaat ggcacagcac 111420tgctgcagaa aggggtctgg tacgctgtga gcttctgtct gaaaaacctt gaccaaacac 111480tggtattctt tggactaagg aagcaacata attccataga acaactgagt gggaaatcac 111540cactgatagc tattgcatca agttctgcaa cagcaactaa gaaatcactg gcaatcactg 111600tgggcaagac aaacactaaa tggtcaataa gctccctgct actagaaaaa cagtggaaac 111660ataaatgaga acaaaatctc tagttgtgca aaggtttttg tggtagaagg agagactttg 111720ttctatgagt tgacctggac ttcatattct tttggaaagg atcagatgtc aaagagtctg 111780ttagtttagg gacaggccca cagtgaaata cctggtaaag caaatagcag ctaagtctta 111840gctgacctct ttcatgggat aagcattgtc aaaaaaagga catgtcacaa caacacatcc 111900ttttgtgttt gtgcagcatt acacagctgc gctggacagc gtaatccatc agctatccca 111960gaaagcattt cacatacagg aaggtttgct taattttgct agacttcaca acagatctaa 112020acttgataag tagactacac aagaagtcca atattctgat gattctgcac tgatgactga 112080ccacgatcca atttttcact tactgtggac tgatttttaa attctgcaac tcgctttaga 112140ttaaccattc atttgacaaa aagaaaaaat ccaacagcaa cagttggctg ggatgtatat 112200taactttttc tggaggaatc tgctgcgcct tctctatcac aaaacaaaaa tattcctctc 112260agcactgagt atatttaacg cagagatatt ttgaaagcca tataattact aacaacatta 112320gtgctctgaa ttagctatta tgacacaact gtagtatctt tgtagatcct gagttgtagg 112380ctgtctatga tggcccaaac atatgattca ggcagatggt acacaaatgc ccagggagct 112440ctcctatagc aagctgtagc atgttgctag acagtttgat agtaaaagga tttaagacat 112500aatcaaaagg tagaggagac acagtacaac ttgttggata atcctttgac ttttgagctg 112560tcaagtatac aaccacggac cattgcagtg agtattaaag cctgtttgaa cagaaaacat 112620gctgattgct agccttaagc aagaaaggga gaaggggcag cagccacaga aacatcttgc 112680agtgtgagga gtgctctaaa ttgtgtgatt aaagatattc accatgaaca gacacattca 112740gtcacttgat atgtcttcca ccagcacaga taccaaaatg gaactcacga cagtggtgag 112800taatttacat attgttgaag caagagaata gctcactccc tttataatag gtttgatgtg 112860atgggctacc aataagagtt aaggcctaat gatctttact caaaagtatt gctgctgcat 112920agcaatgtct gcaccagact ggactgggct atagatggta tcatgtaaca tactagttgt 112980aattaagtgt atcagacaga ctgaggtctt cattattagt attgctctag catcttcagc 113040tgaacaagac taatgaggac tctattaggc agaaaggtat ggactattca gaggctgttc 113100actttcacag acaactaaaa gggttaagga gtccacctct tttctccaga aaacataatt 113160tgttctagac aatttcagag gcattttgta tattgacttt ggagttctgt tttaaaatca 113220gagcatactc agaggtcaaa gtagtttgtt tgttgcccat tcttttattt caaaggattt 113280atgagattgc tttatgcttg ctattgtata ttatgactgt cctgcagacc atgaatgttt 113340cacctgatgt ggcatgaagt tacttgtgaa cgatctgtaa gaatgttctt tgaatgtgca 113400aaagacacat tttgaacctc acatctggtg ctgtgacctg tttgaaaaga acaactcaaa 113460tcaacattca aaactagcag tgagttcgaa tacttctctt gtagcttctg actggagtct 113520gaatatccta atatctgaat ttaaaaagca acagaagtct cttctctgct caacctcttc 113580tgcgacagta catctttctt cagttctgta tttttttttt cttttaatac agatgctctg 113640aatattgctt tcaaaattaa tttggattca tacagtatgc ttgttgatac tttcctactg 113700acaatctgca cagaccatgt tggcacacaa ggtccctgag ttagactgct ccagcaatgc 113760tagactgctc tgcaaaatgc tttatttttt gcaattcagg ctgtaagtgg catcaggcac 113820aagaactaga caattacata caagttttca ctgtaggtat ccctattatt tgcagaggat 113880ttggactaga tggtcttcaa gggtcccttc caaatcaaat gatgctttga ttctgtgatt 113940ttatgaaaag ttgcagtaag tacagggtgg gcataacaca gcaaggagtc ctgaatgtac 114000tgcatttttt atgttctcag aatggtgact gctagaggaa tctggactgt cagtactcat 114060agaggaaaaa aaaaaaaaaa aggaaattga cttaaattcc ttagagacat tgtgtacaac 114120taaatatcac actttttttt ttttgctttg ttttcactat ctgtgccaca gtatttggtt 114180ctgtgcttga attatactta gtgttcaagt ttcagtgaat agcttttatc atttttgttt 114240caatcttatc agtatactcc atccttttct ccaaggtgcc atatgatatc cttccttctg 114300gaacttttat ttagagactt ctttctttct ttccctcttc cattctctct ttctttaact 114360ttttcctttc tcctttcttt tttgttttct tttttttccc ttttatcttt ctttccttct 114420tttttcctta tttctttttc cttctttctt ccttttttcc ttcaatttct tttttccttc 114480catttctttc ttttttcctt ccatttcttt tcttccttcc ttccttcctt cctttctttc 114540tttctctctt tctttcttcc tttctctctc tctttctgtc tttctttctt tccctctttt 114600ttttttttaa ttttaatttt tatttttttt ttgtaaataa aggacttcaa ccaagtaaaa 114660gtgtgtttct gacactgagt tccatccatc attcagtttg gcaaacacag aataggcagc 114720atggggtgtg tcatgacatt atacaggata tatttcaagg agttctgcaa ggctgtacca 114780cgtacagctg agaagctgta ctcttatcat cacaggtgaa gctgataagg taagcatttc 114840ttttggttat gattcatgtt ctaacccatt ttttaaaatg atcataagac ttacaagaat 114900actgatggaa ctttgtggtt tgtcatcaag aacagtcaag aaacaaatga ttaaaggatg 114960acttctttaa aaatctattc ttaccttcac atttctgttc tgcattactg tactgtttca 115020cagcctgcca catatgaagt caaagtgtta gtacaaagta aagctatgtt tactaattct 115080gtaacactga gaagctggca ctgtactgag acaccctttc ttctttttca ttgatgccct 115140ttgtttctga tttagaaatt aaatgcagca ctgaatttgt ttaaattcaa gacttaagct 115200gagttgcatg gtctacctaa catactttct gaatgaagtt actgaatgca gcatggtcag 115260gtatcaacaa catactgcaa attaatttct gtgtattcta aaacaagcaa acgaacaaac 115320aaaaaacaca cacacacatg cacaaagcat ttgcttcaac agtatgtttt ttcaacaaga 115380tcatacatgg agcttaaagc ttaaaatata atactctgtg ggagtagtaa ataatccaga 115440agtttgccct ctatcacctg cacatgtgat tcaattaaga gagagatgga acacatgaat 115500gtgttgattc cacacaatga aacatttggc agaatatctt ggatttcccc tgtacttggg 115560aaattctacc ctaggaagat tctctctgct tgtgacaaaa tgggaagata taaggacctt 115620aatactgcac tttacagcac tgttgtctat tctatgttgt cttctttact aaagagtttt 115680ttttttcctt tactgttaga taaaatgata tgtgttgaaa ctacagggaa aatttcatta 115740gaatgtcaga aaaaaaagac agaaaaaatg tttaaatact gacgatgtga agtatctgca 115800aatgaaacaa gcctaaacaa tcactgcctt attaaaaggt ggattttatg aaaaaggtgc 115860caataaaatt aaagaacaat tttgaaaagt gaggtataat taagtcaacc aagaatggaa 115920catgtaatat ttaacagaca tttgtcataa agcagatgag tttggtaaat cattatctct 115980ttctatcact gtgcttccat ttccctaatc tatttttaag aaggtaatga tgaggtttga 116040gacctctgat aaagtggttg gtataagaat ccagcttcca tttacatgaa ggtggagtaa 116100atccagaaaa aaacttgcgg tgtttttcca gacctaccca ctttatattg tcaataactg 116160tagtttggat cacagagggc tgatctgtta actggtctta aaagtgatgt taaaaactat 116220agtgaaaaac ctggtctgga gtctcaggtg aatgaagact gagaacaaac ctatgtgtgt 116280tttctttcct gcacaagatg ggaaacgatt gtcaatgagc ttctttcaag gcaagtcttt 116340gcaatatttt caacacagta cacatgtaca gaggataact caagtttcaa ataaaacagt 116400tgccagccta cacataactg gtacctatac aagattttga ttgctcacaa atccaagcac 116460acacctgcct tttaaatcca cactactgaa ttctacttac tgaaaataag ctgtgcactg 116520tgtacagagg ttcaagtgca ctgacttcct tggaatacaa ctaatacatt ttaatctttt 116580ctttagacaa cgatttcaga atggattcca tcagtgtaac aaatgcaaaa ttttgttttg 116640atgttttcaa tgagatgaaa gtccatcatg tcaatgagaa catcttgtat tgccctctga 116700gcatccttac agccctggcc atggtctatc tgggggcaag aggtaacact gaatctcaga 116760tgaagaaggt aagttgctta cattggtgta aagtggacag tggactctac ttctgcttgt 116820cattccttct aagtaataac atattatcta ctcatgaggc tctcacatat tttaattcac 116880cagatggatc atgaatcagg gaattgtatt atttttttct aaattctgac atcttccaca 116940taatgtgatc attttttttc catatttttt atttttgtat taaaaagata aaaccctgga 117000ggaaaggaag agggagaaca ttattcgcag tgcataatac acaactaagt taacatccag 117060atgctcactg aaaaaaatat aatctaagca aatagtgcta tttccaattt ctcagaaggt 117120gacatgaagt atgaaccagc tgcaagctta cttgcagcct tttagttcat ctaatctagc 117180atttgttgtg ggtttttttt ttgtttctgt ttttgagcca acagctctac cccgaacatc 117240acgtgtaaat tttaaatgca taccattttt ggtcacgctt gtgttttttt ctcactggca 117300ttttctcttg caggttcttc attttgatag cattacagga gctggaagca ccactgactc 117360tcaggtaaag atgtaacttc tctccttttg ttcctatttt ctcctcagga caaaactaga 117420actactctgc ctctgctcca agcagtttca gactgtcaaa agtggtggca atgctctcaa 117480accaaacaga tctgtggagg gaggaaaaga gtgtgtaact cactcttgtt taaagccagg 117540gaaactgact tggagatagg tttatttgtc tgtttaatgc accatcatca gactaggtct 117600gtgggtgaat tccaccatgg cctgactgtt agtgatgggg acagtccttt ggggtctgat 117660tttctagata aggagaaact aatgtgacat atcatcttgt tttcctgtca tcacctcagt 117720gtggctcttc tgaatacgtc cacaatttgt tcaaggagtt actctcagaa atcaccaggc 117780caaatgctac atactcactc gagattgctg acaaactcta tgttgacaaa acattctcag 117840ttcttccggt gagttgaagt gtgacttaac ctcagtgaga ttgcccactg ggctcacctg 117900ggactcggct ctactgtgag ccacaatggg aattggtttg agccacagga tgagttcaaa 117960cctttctgtg gcttttagga ggaggctagg ctcacacaag gtataagggc tctggagata 118020ttcaagaccc atttggacac tttcctgtgc aatctataat gaacccctgc agggggttca 118080aattgatgat ctccagagat cccttccagc ccctgcgatt ttgtgactct gtaatatatg 118140cccatgcagc aactgctaca gggagcaatc aaaattgctg ctcattcact aaaaaatgtc 118200tcttaatgaa aaaggtgatt tgtaagggag gaaaatgact tgaaagcgtg acgactgaaa 118260ttgacaaaaa tattttgttc atcttttcta aaactagaca taaaataact cacttaaaga 118320aagttttggt tttgaaataa aaaacaggaa tgtaagaata cacagttcaa aagaaaaggt 118380aggcacgaag atgaggaaat gagtattgtc tgtccttaat aatgtttgca gaacagaagg 118440ttttatggta aaatgaagaa aatatttcaa aattttaact tagaatccaa tctgaagaca 118500aaagtgacaa atctaaatat gtgaagtagc cttgtccagc tttaagattc agttacagca 118560agagagctgt ttgacttgtt caagtgtagg gatagaagtt tcttttaacc atcactttcc 118620atttcattaa ttttgcattt catattcttc tattttaaag ttctcaacag tcaaacacaa 118680ttcttctgct tataggaata cttaagttgt gcaaggaagt tctatacagg aggagtggaa 118740gaagttaact tcaaaacagc tgcagaagaa gcaaggcagc tcataaactc ctgggtggaa 118800aaagagacaa atggtaagaa gtaaaaaaat agctgatatt ttctcctacc tactgtaatc 118860tacgctcttg tcttcttctc ctcaaaatgt gaagaaaggc atatcaagga acagcacttg 118920attattgcta tgaaagcaaa ctcccataaa actcaccatg cccttcattg caggcattca 118980tgcaaaccag acaggctgtg tcttaacact cactgctttg cttccttttc acaggacaga 119040tcaaagattt gcttgtatca agctccattg attttggtac aacaatggtc tttattaaca 119100ccatttactt caaagggata tggaaaattg catttaatac agaagacact cgggaaatgc 119160ccttcagcat gacaaaggta gggacatggg cactactact ggaaaaattc aagataaagt 119220gatccctact cacattgtct catgcttctg ttttgcagga agaaagcaaa cctgtgcaaa 119280tgatgtgtat gaacaatagc tttaatgtgg ccacactgcc tgcagagaaa atgaagatcc 119340tggagctccc atatgccagc ggagatctga gcatgttggt gctgttgcct gatgaggttt 119400ctggcctgga gcgggtacgg ccctggcagg ggaagccaac tagttcggag ttcagtggga 119460acttctgact gctttcagac ctttggctgt cctctcaccc cctggctgtg ctgtgctggc 119520caggcagggg agcacaacag tggcccaggt gcttctaggt gctcaggcag aggttggcct 119580ctaaggagag ccctagcctc aatgttatta aacaaagagt gtagctaaca aaacaaaggt 119640aaaggagcct agggctgctg tagtcctgca gcaggggatg ttggtatatg caagttatct 119700ccatcaagta ctagagacag atatgctagc aggatttctt tttttacttt gaagaaattt 119760caattcccag agatcaagta gagttcaaac actgttacca agtcataggg accaattctg 119820ttgatgaccg ttaatagatt tttttcatga gtcacccctc caaataatta aatataattt 119880tttttttgta aatatgaggg atattttaaa tgatcatttc tcattgaatg tagaaaaaaa 119940taggaaaaat ataacaagaa aacaaacagc atttctgaga ggttagctgc aaacatctgc 120000aaatgagcaa aaatttgatt tgacataatc aaaaactgat ttttcagaaa agcatttgat 120060ctgttggaag aattttcaga tgacaaagtt ttggagagct tcatcaagac agatgatatg 120120taggctatag tcaggaagaa gcacaaggga taaacaaata gatttaagct taagcgtcac 120180ttctgttttg cacacaaata aatgaaataa atagcaacaa gtggtattaa tacagttggt 120240atggccacca tactcctgct ttatgcattt cattgtctct tctctttgca gattgagaag 120300acaattaact ttgacaaact cagagagtgg actagtacca atgcaatggc aaagaagagc 120360atgaaagtgt acctgccccg catgaagatc gaggaaaaat ataacctcac atctatatta 120420atggccttgg gaatgactga cctgttcagc cgttcagcca

atctgactgg catctcttca 120480gtagataacc tgatgatatc tgatgctgtc catggggtgt tcatggaagt caatgaagag 120540ggcactgagg cgacaggttc aacaggggca attggaaaca tcaagcattc ccttgagtta 120600gaagagttta gggctgacca tccattcctc ttcttcatca gatacaaccc aaccaatgct 120660attctattct ttggtagata ttggtcgccc taaagagaga aagagctgga aataatgctt 120720accttcccct cagaaatcaa acctctttac tgtagtattg tagcataatc tcaatgcaat 120780attttatcca agtggaaagc cttcaatatc tagggagaca ttcttgaaga agcatgtgaa 120840atttcagatc tttatatgca ggaatttatt ctcagcttag attcaggatt catatccaag 120900gtgtacatat tcccaatgtg cttgaataac ttgggaaaca gggccagtgc tttggggttt 120960tttttgtttg tttgtttttt gtttttttgg tttggtttgt ttttttctgg ttggttgttt 121020tttttttttg tgtgtgtgtg tgagattctg ccattgttat tgagaatctg gtttctctat 121080aggagttctc tgaaataaac acagctttca ggaaaatcct ggtcctttcc attgaattag 121140ctgggcagtc atcctagaac tgatgcctgg acaacttgca gatgaaattt ttaacttcag 121200cagaccattt gtcttccagt aatccatttg gacttattcg tgctgcgtaa cattttttct 121260gagggagcat acagaaagtc taccatttct tcttaaatca tctccaaaca aaacatcttc 121320ctgattgata ttatttccca ttttcatccc agtgacatgt cactgatttt gtgaatgtta 121380attaatggtc tttctattta ttctaataaa agcttcgcaa acaaaacatg tcattaccta 121440ttctgggtta ctgtactaca caacctgaaa aatacgatat agcgggtaat aattattgac 121500agaggtgact aagctggtat gtggatccta ttttcaaaat cagaatgtac ccatatatga 121560ggtcactaaa tattttaaga ttaaaaaaaa aaaaaacaac tgggtttaat caaggtaaac 121620cctatagctc ctactcttca attgagcttc tcccaataca gcataccaaa taacaaaatt 121680ttttgaattt actgaatttc cagagaactt ttacagaaat cctctaaggg tcctcagtaa 121740atacatgaag gtgatgtgta caagatagaa ttttaaaata tgagaaaggt attaaaaggt 121800agactgcttc agctttctca tgctgacaag aatcacatga agaaatcttt ctattgcctc 121860atgtgatatt cctctcgaga tgttgtatgc tatttcacgg tctttagagg aaagggtctt 121920taggttatat attatccaat tataatggtt actagtgtta atgacagttt tctgctagga 121980tacacatgca gaattgcaaa ttacagtatt ggtactgaaa atgtagcgat acttcagtaa 122040ttcagagctg cctcaaacac atgccatgtc agcattaact ataacttgaa atgatgacat 122100tagcagcagt gaaacacgtt tcatacccac taaaaatggg agaaatgcga ttactggtct 122160tcccaagagg gtcagaagga ttaggtacag tttgcacaag gattcaagta aaaaaaagta 122220tttcaagagc tatgaaattt caagttttac tgtgtacatc atgttatttc cctcttacag 122280ctgaaatcag tcaatacagt tttgctacaa ctaaaaccaa ccaaccaaac aaacggcaaa 122340ggaaaaaagg caggactggt aaaatattgt cctgagcagc agagtgtgta aggttactaa 122400gcagttataa tactgtgttg gaactgaaat atgcagctat gtcactctgc actttctagg 122460tacaataaca gtaaaagtac ccacatttac tatggaggta tttaatatat agcgtaaact 122520aaaaaaacag ctattcaatt gtttgatctt tttaaagcaa aagatgaaga aaatcaaggc 122580agaaatgata aatgaatttc aaaaactata caaaagaggg tattcaaggc acagagtcca 122640cctaatgtct taactgtgaa atggaagcat ttgacttgtt ttaaaaaggc cattaggtgt 122700cactaagggt aaaaagttac gtttatcagc tttcagaaag aggatggcat tccaaaggtg 122760cctctgagct ctgaagccca gcaagggata agagaaatga atcccaagtc cagctattgt 122820ccaaaagtcc tttttgttcc ctgataccat gactgaagtt gtcatgtcaa gacattgcct 122880tctgtctgtt cactacacct catgttctct cagtgctgtg ttcttagaga ggcagtactg 122940ctagtggtcc gcggaatgaa aacagccagg tgtaatcaca ctcttttgaa tgcctcatga 123000gaagtgctct ctggactaag tgtaactttc cttctcacat catttggaga agggaccatc 123060acagaatcat ttggagacca cctgcatcta ttctgcactt ctctccacat ttgcctatcg 123120tcttctaagc aaaccgaatc tatggctgaa gttacaagac tctgcatgtg gtgtcacagt 123180caccagaggc agaggactca agagaatatc ctggtccaga gctactctgg gatctcaaaa 123240gtcacttggg aaagcacagc atgttgaact agactttggt gtatttttcc aatctttatc 123300agtctacaaa atatcaactg gacatggagc agtggttcac tttggtgttt cctgtggctc 123360atgatctaag attcttcagt ctggaaaata aactgcagag attactgttg aggagcagca 123420agtgtcagtc tgctcagcaa gggaaggaga ctgagggcaa gggaaggaga ccgtgccagt 123480aaggctagaa gggcccgata agggatcaag tgctaaattt tccaatatta tagccatagt 123540ttagtgattt ccagagaaat atgtagtgat gtggtaggcc aaagatcaaa ccagaaggcc 123600ccagaatggc aacaggtgat gatccctgtg gcacattctc tatcttgaag taaaacagca 123660tggatccata taaatacatt cttgctcaac agcagaaata acaaacagta ttgcttactt 123720ctacgaatat cctaacaaaa catgtagatc acaatgccac tgaacctttg tatggatgga 123780atctgtgcaa tctgccatga ctaaagctct gtccaaaact gcacaactta gggtgcccag 123840cttctgaagg gatgtgaaat tatctgtgct atctcctttt cccttcttgt gttagctcca 123900gtaaactcta ttttaagaaa taccttacag tttctgattg tcttctttac tggtatccaa 123960agggactcct atgcattaca gggtcctcca gcacagtgag gttcttggcc tggtgcaggc 124020atgcaaagta gcttaggcac gggtcacaat caagatactc agtttaatgc ttctcccaag 124080tgatgggatg ctaaaatctt acatgatttt aaaaggaaag tgttcaaact gtggaagaga 124140aatccactga caaataagaa agatacagaa aataaagtta gctatagaag acatggaaca 124200ggaaataatg ttagaactct gagggcaaga gtaagcctta acagtaatga caagcctcac 124260tggaggagct cttccacata cgttgttctc atgggcccag gagtctgtac tggaaattgg 124320cacacagttt gggtaccggg ggcgatcttt gtgagatgaa gccctgaact gccctgggtc 124380agctgcaggt gtctctgtaa tggatgaaaa caactcactg tgcaccagat tttcagctaa 124440taagaaaagc acatggcatc tctgctcaaa cagaatcata gaattgctca ggttggaaaa 124500gaccttaaag gtcatcgagt ccaactgcaa cctaaccaac taccctaact ctaacaaccc 124560tcctctaaat catgtccctg agcaccacat ccaaacagtt tttaaacaca tccagggatg 124620gtgaatcaat cacatccctg gggagcctat tccagtgctt aacaacactt tctgtaaaga 124680agtttttcct gatatccaac ataaacttac cctggcacaa cttaaggcca aatttaatta 124740gaaaatgtag cagcactgca atgtagcaaa tgtaattacg aaaaggtggt agctgctagg 124800gacagaggac atgcaaatag acccaaaaga taaagactag aaacagaaaa aggggacatg 124860tgagaggtat gtttggagaa acataacaga ggagatattt gaaaggagat cttgggagca 124920caggcaaaga cacaatcctg ggaggaggtg ctccatgcta gaggatgtac ctctaaggca 124980ccgcagccat gggcaaccaa cacaggtcag cgtcatcctg gtgagactgt atcccacaag 125040cagctaacac tggagtaggg acagccccga agaactgcag cccaggcagc acactagagc 125100agagaaatct agttagcagc aaccactggc agacagaaat gattatatag attacatact 125160gaccctagcc tcttacactg cctactgcat cactgaaagg actgggaaga agagagtgca 125220ataacgtagc tgaaactagg aggaaggcaa ggagaactga agctgactag ggaaaagggg 125280gattaaaggt ttaagtgtct attccatagt ttgctggttt gttttttgtc aattcctgaa 125340tcagtaattt ttatgttaat tagcaaaaaa ttacaaacac tccccaagtc aggactgtta 125400cctacaacag aagctcagat cagctgagcc ttagtctttt ggtccctccc tagggaatgc 125460tgtatgtgtc tctctctcca ggcctgctca aaattgacct cagacccaaa cttttgctga 125520atctccagta ccacctcttt tgctcctaac tagataacaa agccctgagc gctttgcttt 125580tagcaaagcc tttaagtgcc attaccaact gcacctggag cctttaccta cccctatgga 125640cccaggctct atatttaagc tctgccctga accttcactt ctttcctgtc ctaagttaga 125700tgtactagta tggtgtgtac tatgtctcca gttcaaacac agctgtgccc atacctggcc 125760aaggactcct agtatgacct gggctgtgcc ttgctgctaa ggacctgctg ggtgattgct 125820ggacctgatc ctaatcctga attaagaaat gatttcttgg cttgactgga tgtgccctgt 125880ggtatgatac tgccttatga tttggactct tgtttgcagc tgtgcaaatc cctaaggagc 125940ccagtctctg gccacctgga atcttgtcac taccaaactt cctgagggac tggtcttgct 126000ctgggttctg atctctggac agtactcacc ctttactcag cccaggctcc cagttaagcc 126060cctttccacc ctgccaggct ctccgctcca tccctagcag gggctctcat gacagtgtga 126120ccccccctta ctcaggtcag ggccacttgt gccacgttcc tttcctgtct tctgtccctg 126180ccttggctct aaagcagtgt gctaccatcc acaaccactg catctctcta aagtaagcct 126240ctcctgagcc caagtctctg taacgaggaa ggatgcactt tgctcagaag gatgcgaggc 126300tgcttctgag ctctgagggc actgacctcc catgaggtac accccatacc caggaccaca 126360attcagcctg ctggaaccat caactcctgc tggagtaagg ccatagcaag accagcatcc 126420acctccctgc agccctgccc tgcccagata ttgggcctgc tgatctcagg atgcagactt 126480gcttctcagc ttgacctaag cattgccctg tctttatgga cccacctggt tagcaagttc 126540agtgcagaag gaggctgttg gcatctagct aattttccac ccacattact gtctgctgac 126600tcattctacg tctctcccat cttgttacaa taataatttg ggagatcata ttgaaggtct 126660taataaagtc aaggcatgtg atattctctg ctttgccttt gtttctagaa taagccactt 126720catcatagaa gatgaaaatg ctgatcagca gagatctgtg cttgataaat ccatgctggc 126780ttttcctatc accttatatt ccttcatatg ccttgagaca cccaaggagg ccttggatca 126840gagctgtctg tagcagtcct aactggtata caattagttg tacaacaggt agtgatccgc 126900ataatagttg gcgtgagaaa gtgggcctgt gctgtgtcaa gcatagagtt tgggttccag 126960tcctgttctg catggcacat atgcctgagc agctgggtaa tctctgcatt ccaattggaa 127020ggcaggggcc tgtaggcagt tcccacttgg catgggtgat tgtaccacct gtgtcctcat 127080ctgtgaagca tcatgttttc attcaaatat ccttttgttt gacagtagaa atgaacagaa 127140ttgttttttt ttcctaagca aattctgcaa gagctctgaa gaacaaggtg tcagtgaact 127200tctagctcca tagataggac ttgcatcaca tgtcatgcct tgattggagg tctatccgat 127260actgaacaac ttgtggttcc ctgagggaat gtaagattac tgatactact ctctctttat 127320gttagctaca ataaatggta ggttaagcaa tagatacaga gtttgagtgc ctttcttaca 127380agcatcatag tgaacaaatc cactggtgat ctaccttttc aataactaca gagaattgta 127440atctcttgga ttctcctcct tccccgttct gaaaatgtgt tctttttttc caaatcagaa 127500accttcctca accaccctga ctattctttg gacattgttt tgttcttgct cctaaatagg 127560ctttataatt tttgtaagtg aaaggctttg catgcaggtg aggctacaac tcattcagta 127620acaatgagga agactgtcag attttgggga aaattctccc acccaacctt ttgctagcca 127680gtaagatgta atcactgaat gtcatgccac aaagaccata ccaacatcag accacatatc 127740tacaggaagc tttaaggaat cattgactgt acagtgaagg gtaaatcaaa ttaaaatgaa 127800tgtgaggtct gatacgagat atcctcatgg gaatcaagag caaagacaaa tagtttttca 127860cagtcttgtc atgatctgtc acagaccaag gcagcacagc aggcaacaat gttggtctct 127920tcagaatggc acagcaccgc tgcagaaaaa tgccaggtgg actatgaact cacatccaaa 127980ggagcttgac ctgatacctg attttcttca aacaggggaa acaacacaat cccacaaaac 128040agctcagaga gaaaccatca ctgatggcta cagcaccaag gtatgcaatg gcaatccatt 128100cgacattcat ctgtgacctg agcaaaatga tttatctctc catgaatggt tgcttctttc 128160cctcatgaaa aggcaatttc cacactcaca atatgcaaca aagacaaaca gagaacaatt 128220aatgtgctcc ttcctaatgt taaaattgta gtggcaaaga ggagaacaaa atctcaagtt 128280ctgagtaggt tttagtgatt ggataagagg ctttgacctg tgagctcacc tggacttcat 128340atccttttgg ataaaaagtg cttttataac tttcaggtct ccgagtcttt attcatgaga 128400ctgttggttt agggacagac ccacaatgaa atgcctggca taggaaaggg cagcagagcc 128460ttagctgacc ttttcttggg acaagcattg tcaaacaatg tgtgacaaaa ctatttgtac 128520tgctttgcac agctgtgctg ggcagggcaa tccattgcca cctatcccag gtaaccttcc 128580aactgcaaga agattgttgc ttactctctc tagaccccca agtcaaacca actatgcagg 128640tatgctgaca acactatgat gacagcctgt tctgatcaag atctcatttg ttcatggaca 128700atttttgttg cttgcagctg gtcttccatt gggaaagagt gtagtatatc cttctcatct 128760gacagaaaag cagaaattct catgctccac acttaatcta cattgtttta aaccaccggc 128820tacttcttgg agaggaaaaa tggcttttat aagactcaca aaacaaagct ctgcaagtca 128880aatgcataca aaactgttct gtaggtctgg aatcaggaca ctatgtggaa gtcaaataga 128940gcagctttaa aaagcctttg ggatcattct catcttatat ttgcagcacg atactatgac 129000agtgataact gacataactg catcaatttc cttgatattt tatttgtctt aaagtacaag 129060acatagagat ggacgtaaag atggacatat gactcaggtc tggacaggtc cgtggtccat 129120gtatgataaa agagatgaag ggaaggagaa ttgagactgt ctaagaaggg cttcagggac 129180gttctgaagg cagatttgac tgaatcagat gtactgtcca agtctcatat gtagcaatgg 129240aaggctgata ttggagaaat ataaagaaat ggctgtgaac tcaaagtgac cctgaacaga 129300aaagggatat ggagttaaaa taatgtcaca gaactgaggt ttatatgata taccatgggc 129360tgcagagggt cagagtgctc caccatgggc ctctcttggg ctgcagggaa cttctgttct 129420acacctggaa cacctcctgc cctcctccgc actgacctca gtgtcatcag ggctgtttct 129480ctcacatttt ctcactcacc tctcccaact accattgtac agcagttgtt cttacatatt 129540gctcctcctg aggtacatct agcatcgatc actggctcag ctctggccag tggcagctcc 129600cttttgagga cacgggacag ctgctgggct ctgttcacag aggccactcc ggcagacctc 129660cactaccaca acttgtagtg taaatccact acaactttct gagctacaga aatgaaatgg 129720agaccctctc tgctatggga tacaaaagag gaaacgtggc gtttagctct ggctcactgg 129780tacacccaac cacagggtga gaagcagcct gttgttattc actactctta ggacagatta 129840tggtgaattg ttaataaaag catttcttca taacatccaa aggaggaaat acactaaatt 129900atatttttta ttaattaatt acacatgctt aattatatat ggcatggttg ctttggaaga 129960atcttgtcct tactgaccag atctgctgtt tgctgagaca aaatggctga caattttggc 130020catggtggat accttccccc ttttctgtag cattaggaca gaagttattc tggagcctgt 130080ctgacaagtt agacttgata cctttaagta tttggaagtg tgcttttcat gctggatgtc 130140atctccagaa cctccctgtc tggtaagcag ttccctgcct tagtaagagc cgaaacggtc 130200tctcttttcc ttgttatctc accaggatat tacaatgtga caggactatc tgaactatgc 130260caacctgcaa attccaaata tatatatata tataagatat ctatacacaa attattagtg 130320tttgattgac accagatgac agagaagtgc atctgagaaa acctattccc aatctccttt 130380ctctttctgc agactgacat gcatttcata ggtagagata acatttactg ggaagcacat 130440ctatcatcac aaaaagcagg caagattttc agactttctt agtggctgaa atagaagcaa 130500aagacgtgat taaaaacaaa atgaaacaaa aaaaatcagt tgatacctgt ggtgtagaca 130560tccagcaaaa aaatattatt tgcactacca tcttgtctta agtcctcaga cttggcaagg 130620agaatgtaga tttccacagt atatatgttt tcacaaaagg aaggagagaa acaaaagaaa 130680atggcactga ctaaacttca gctagtggta taggaaagta attctgctta acagagattg 130740cagtgatctc tatgtatgtc ctgaagaatt atgttgtact tttttccccc atttttaaat 130800caaacagtgc tttacagagg tcagaatggt ttctttactg tttgtcaatt ctattatttc 130860aatacagaac aatagcttct ataactgaaa tatatttgct attgtatatt atgattgtcc 130920ctcgaaccat gaacactcct ccagctgaat ttcacaattc ctctgtcatc tgccaggcca 130980ttaagttatt catggaagat ctttgaggaa cactgcaagt tcatatcata aacacatttg 131040aaattgagta ttgttttgca ttgtatggag ctatgttttg ctgtatcctc agaataaaag 131100tttgttataa agcattcaca cccataaaaa gatagattta aatattccaa ctataggaaa 131160gaaagtgtgt ctgctcttca ctctagtctc agttggctcc ttcacatgca cgcttcttta 131220tttctcctat tttgtcaaga aaataatagg tcaagtcttg ttctcattta tgtcctgtct 131280agcgtggctc agatgcacat tgtacataca agaaggatca aatgaaacag acttctggtc 131340tgttactaca accatagtaa taagcacact aactaataat tgctaattat gttttccatc 131400tccaaggttc ccacattttt ctgttttctt aaagatccca ttatctggtt gtaactgaag 131460ctcaatggaa catgagcaat atttcccagt cttctctccc atccaacagt cctgatggat 131520tagcagaaca ggcagaaaac acattgttac ccagaattaa aaactaatat ttgctctcca 131580ttcaatccaa aatggaccta ttgaaactaa aatctaaccc aatcccatta aatgatttct 131640atggtgtcaa aggtcaaact tctgaaggga acctgtgggt gggtcacaat tcagactata 131700tattccccag ggctcagcca gtgtctgtac atacagctag aaagctgtat tgcctttagc 131760agtcaagctc gaaaggtaag caactctctg gaattacctt ctctctatat tagctcttac 131820ttgcacctaa actttaaaaa attaacaatt attgtgctat gtgttgtatc tttaagggtg 131880aagtacctgc gtgatacccc ctataaaaac ttctcacctg tgtatgcatt ctgcactatt 131940ttattatgtg taaaagcttt gtgtttgttt tcaggaggct tattctttgt gcttaaaata 132000tgtttttaat ttcagaacat cttatcctgt cgttcactat ctgatatgct ttgcagtttg 132060cttgattaac ttctagccct acagagtgca cagagagcaa aatcatggtg ttcagtgaat 132120tctggggagt tattttaatg tgaaaattct ctagaagttt aattcctgca aagtgcagct 132180gctgatcact acacaagata aaaatgtggg gggtgcataa acgtatattc ttacaataat 132240agatacatgt gaacttatat acagaaaaga aaatgagaaa aatgtgtgtg tgtatactca 132300cacacgtggt cagtaaaaac ttttgagggg tttaatacag aaaatccaat cctgaggccc 132360cagcactcag tacgcatata aagggctggg ctctgaagga cttctgactt tcacagatta 132420tataaatctc aggaaagcaa ctagattcat gctggctcca aaagctgtgc tttatataag 132480cacactggct atacaatagt tgtacagttc agctctttat aatagaaaca gacagaacaa 132540gtataaatct tctattggtc tatgtcatga acaagaattc attcagtggc tctgttttat 132600agtaaacatt gctattttat catgtctgca tttctcttct gtctgaatgt caccactaaa 132660atttaactcc acagaaagtt tatactacag tacacatgca tatctttgag caaagcaaac 132720catacctgaa agtgcaatag agcagaatat gaattacatg cgtgtctttc tcctagacta 132780catgacccca tataaattac attccttatc tattctgcca tcaccaaaac aaaggtaaaa 132840atacttttga agatctactc atagcaagta gtgtgcaaca aacagatatt tctctacatt 132900tatttttagg gaataaaaat aagaaataaa atagtcagca agcctctgct ttctcatata 132960tctgtccaaa cctaaagttt actgaaattt gctctttgaa tttccagttt tgcaagccta 133020tcagattgtg ttttaatcag aggtactgaa aagtatcaat gaattctagc tttcactgaa 133080caaaaatatg tagaggcaac tggcttctgg gacagtttgc tacccaaaag acaactgaat 133140gcaaatacat aaatagattt atgaatatgg ttttgaacat gcacatgaga ggtggatata 133200gcaacagaca cattaccaca gaattacttt aaaactactt gttaacattt aattgcctaa 133260aaactgctcg taatttactg ttgtagccta ccatagagta ccctgcatgg tactatgtac 133320agcattccat ccttacattt tcactgttct gctgtttgct ctagacaact cagagttcac 133380catgggctcc atcggtgcag caagcatgga attttgtttt gatgtattca aggagctcaa 133440agtccaccat gccaatgaga acatcttcta ctgccccatt gccatcatgt cagctctagc 133500catggtatac ctgggtgcaa aagacagcac caggacacaa ataaataagg tgagcctaca 133560gttaaagatt aaaacctttg ccctgctcaa tggagccaca gcacttaatt gtatgataat 133620gtcccttgga aactgcatag ctcagaggct gaaaatctga aaccagagtt atctaaaagt 133680gtggccacct ccaactccca gagtgttacc caaatgcact agctagaaat cttgaaactg 133740gattgcataa cttctttttg tcataaccat tatttcagct actattattt tcaattacag 133800gttgttcact ttgataaact tccaggattc ggagacagta ttgaagctca ggtacagaaa 133860taatttcacc tccttctcta tgtccctttc ctctgagaag caaaatacag cagatgaagc 133920aatctcttaa ctgttccaag ccctctctga tgagcagcta gtgctctgca tccagcagtt 133980gggagaacac tgttcataag aacagagaaa aagaaggaag taacagggga ttcagaacaa 134040acagaagata aaactcagga caaaaatacc gtgtgaatga ggaaacttgt ggatatttgt 134100acgcttaagc aagacagcta gatgattctg gataaatggg tctggttgga aaagaaggaa 134160agcctggctg atctgctgga gctagattat tgcagcaggt aggcaggagt tccctagaga 134220aaagtatgag ggaattacag aagaaaaaca gcacaaaatt gtaaatattg gaaaaggacc 134280acatcagtgt agttactagc agtaagacag acaggatgaa aaatagtttt gtaaacagaa 134340gtatctaact actttactct gttcatacac tacgtaaaac ctactaagta ataaaactag 134400aataacaaca tctttctttc tctttgtatt cagtgtggca catctgtaaa cgttcactct 134460tcacttagag acatcctcaa ccaaatcacc aaaccaaatg atgtttattc gttcagcctt 134520gccagtagac tttatgctga agagagatac ccaatcctgc cagtaagttg ctctaaaatc 134580tgatctgagt gtatttccat gccaaagctc taccattctg taatgcaaaa acagtcagag 134640ttccacatgt ttcactaaga aaatttcttt ttctcttgtt tttacaaatg aaagagagga 134700caaataacat ttctctatca ccgacctgaa actctacagt cttcagagaa tgaatggctt 134760gctaaaagaa tgtcaaatct tactatacag ctatttcata ttacactact aaatacacta 134820taaggcatag catgtagtaa tacagtgtaa aatagctttt tacactacta tattattaat 134880atctgttaat tccagtcttg catttcacat ttgcaaaacg ttttgaaatt cgtatctgaa 134940agctgaatac tcttgcttta caggaatact tgcagtgtgt gaaggaactg tatagaggag 135000gcttggaacc tatcaacttt caaacagctg cagatcaagc cagagagctc atcaattcct 135060gggtagaaag tcagacaaat ggtaaggtag aacatgcttt gtacatagtg agagttggtt 135120caccctaata ctgagaacct ggatatagct cagccagcgt gctttgcgtt caagcttacc 135180agagctgttg tatgcctgtt aagcagggca tacagtcatg aggctcttga aaaatcttaa 135240cagacaaagg gcaatggaaa atcggagtta agggatggta gggataaaat gcatagaaag 135300aggtaccaca attttgattt ttgccctaat gcctctctgc gtggttcctc aatttttcta 135360cttcattcct catctcctca gagcattcct ttccctcatg cttgaaacac agatgaaaga 135420ctgtgaattc taactgagat gaaaacatcc acaaccacac aacctctggt gtggagtcac 135480attctgtgaa ggcaaaaact aggccacgta atctatgtgt

gcaagctacg tgtaagctat 135540gtgtgtgaca ggacaatgtg aggaacatac tatgtgcaca aggactgcag aataaacagg 135600agcaaagttt ttgaagaaaa cagagtaaaa tcctgttttc ctcttttgtt acattcttta 135660catatatctc aaatttcctc tttggttaga agcaagtaat atttatgttt cttggtactg 135720tttgggttga agaccattct gggataagag aaattccagt ggttcttccc ctaatcataa 135780aatgtacagg tttagttttt ttgtaacaca gaaatctctt catcttttat cttttgttgt 135840gattctttat agagagagaa acaagactta ctgacaatag cagcaagaaa atcaatcttg 135900gaagaacaag attgcagttg caaaaacaaa ccaatgtcct tgcccctaca tcctcttccc 135960cataaattct acattctcta tctaccttgt gcttgccaac atgatatacg taaactctct 136020tttcgtattc attcttaaag gaattatcag aaatgtcctt cagccaagct ccgtggattc 136080tcaaactgca atggttctgg ttaatgccat tgtcttcaaa ggactgtggg agaaagcatt 136140taaggatgaa gacacacaag caatgccttt cagagtgact gaggtatatg ggcatacctt 136200agagatgtaa tctagaattt atgaagagag tagacatgtt gttatatgaa cactgcatta 136260gcgtatctgc tcatttgtct gcatctcttt cagacactgt gttaaaagca gggaattttc 136320cttatgtctc tctcatcaca atattcctga cattgcaaag ctcctgagaa ataacttcag 136380attcccactt ttcctaggaa ggtcttcctg gatgagaaca atcaatcatc ttaactgtaa 136440ctagatattt ctgcatctaa gaataatctt tgttaaaact atattctctc tctctttttt 136500tttttttttt ggttctccag caagaaagca aacctgtgca gatgatgtac cagattggtt 136560tatttagagt ggcatcaatg gcttctgaga aaatgaagat cctggagctt ccatttgcca 136620gtgggacaat gagcatgttg gtgctgttgc ctgatgaagt ctcaggcctt gagcaggtat 136680ggccctagaa gttggcttca gaatattaaa aacacatgga aatttagctg ttgtaaagct 136740cttttcaaca cagttatcct aaaacattta accagcacaa atttcatcat gattcaatat 136800gtgattgttg catagaagtg tagatttgtc ccactgggtc ctgcaatagc ccatgctgag 136860catggcttgc tgaaagaact gctttagagg gtgaaaagtt tgacacagca gacaagatga 136920ttctcaccta agcagctgtt actgtagtgg cttgaactct aaaggtcttg tatctccatt 136980cctgtgcact gaggagcttc ttggaaagtt catataaggt ttactagttc taactattat 137040ctcatttggt ggcactcaat gtgctttgtt cacgtcttca taaattaatc tatctaaaaa 137100ttggatgtgg ttaaagcaat ttcagaaata acatgtacat aatgtacaat tattgatatg 137160aacagaacac aggcatagca tattgtaatt aggaggactg tagttatttt gaataggaaa 137220cacaatgtaa taaatgagaa ttcattgaaa tgttagtatg ctaactcaat ctaaattata 137280aagataaaga ggcatttaat cacagctaga tttccatcac ttgtgacaga caggcatatg 137340aatgattatg tacagctcta ggaaaaaaag tatgtaggaa aactagtaca ttttgattag 137400aaagtctgaa aatgaggtgc cttgatcaaa gagaatacgt gtgtttgaga aaaaaaaagt 137460ttggatagag gtggtaagag agaatatatt gaaatggtgt ttctacaaac tgccatggcc 137520agatttgtgt aagagacatt cagtaagtag gcaaggaaag aaatattact aggtacaaag 137580caacattagt aataccaaaa gaaaccaatt attccagatg ccaatctcgt aatagggtta 137640agagatttcc acccctctag tagtcaccag tgcaaccagt aactttgcta atttacattt 137700tcttttttta aatggcagat atagctttga actgagtgat catgaactgg tactgtgtaa 137760ataagatgga agcatacttg ggagctaaac ttctagtttt taaaaactca aattctcttg 137820aaagatcagt tcccagtcta gtaacagctg atagtttaag tatcagtaat tggctaccat 137880taacaactgg ctcctgagag gtcttaaatg tagagacagc tttaaactca aaagcacaga 137940gtgattttta gaatagattt cccaagcaaa gaaaataaac agggaggagc tttaagggag 138000tagccatctc attattatta ttatttaaag aaatggcagc aaggctataa aagaaaaata 138060agacagagca gagaagaaag agtcatggta tgcttttcta tcttagcaaa attaatctct 138120acatgcctag gaaaaagcca tgacaagagc aatcagttca aaaggtgtat gcaaaaaaac 138180acataatagt aactagtact gcattgccag gaaggaagtt atgtcgccat tccatggatc 138240tcattctcat ttccttgcag cttgagagta taatcaactt tgaaaaactg actgaatgga 138300ccagttctaa tgttatggaa gagaggaaga tcaaagtgta cttacctcgc atgaagatgg 138360aggaaaaata caacctcaca tctgtcttaa tggctatggg cattactgac gtgtttagct 138420cttcagccaa tctgtctggc atctcctcag cagagagcct gaagatatct caagctgtcc 138480atgcagcaca tgcagaaatc aatgaagcag gcagagaggt ggtagggtca gcagaggctg 138540gagtggatgc tgcaagcgtc tctgaagaat ttagggctga ccatccattc ctcttctgta 138600tcaagcacat cgcaaccaac gccgttctct tctttggcag atgtgtttcc ccttaaaaag 138660aagaaagctg aaaaactctg tcccttccaa caagacccag agcactgtag tatcaggggt 138720aaaatgaaaa gtatgttatc tgctgcatcc agacttcata aaagctggag cttaatctag 138780aaaaaaaatc agaaagaaat tacactgtga gaacaggtgc aattcacttt tcctttacac 138840agagtaatac tggtaactca tggatgaagg cttaagggaa tgaaattgga ctcacagtac 138900tgagtcatca cactgaaaaa tgcaacctga tacatcagca gaaggtttat gggggaaaaa 138960tgcagccttc caattaagcc agatatctgt atgaccaagc tgctccagaa ttagtcactc 139020aaaatctctc agattaaatt atcaactgtc accaaccatt cctatgctga caaggcaatt 139080gcttgttctc tgtgttcctg atactacaag gctcttcctg acttcctaaa gatgcattat 139140aaaaatctta taattcacat ttctccctaa actttgactc aatcatggta tgttggcaaa 139200tatggtatat tactattcaa attgttttcc ttgtacccat atgtaatggg tcttgtgaat 139260gtgctctttt gttcctttaa tcataataaa aacatgttta agcaaacact tttcacttgt 139320agtatttgaa gtacagcaag gttgtgtagc agggaaagaa tgacatgcag aggaataagt 139380atggacacac aggctagcag cgactgtaga acaagtacta atgggtgaga agttgaacaa 139440gagtccccta cagcaactta atctaataag ctagtggtct acatcagcta aaagagcata 139500gtgagggatg aaattggttc tcctttctaa gcatcacctg ggacaactca tctggagcag 139560tgtgtccaat ctgccgctgc cctgatcctg gctggggtga tgggacagac cttggctgcc 139620actgagacat ctgagacact gagatctgtc tcaactcaga tttacccaag aacagatcat 139680tgccaacaga acaaaatctc aaacttatgg ctagtgatga cagcagtcag ttgtcccatc 139740tgtgacccac caaggctggc atgctggaat gagcaggctt tggtggcttg tagttactgg 139800acagcaccac tgacatgggc aggggaaaaa ctgagcatgg tgtaaatcac tgcctcaaag 139860ccacttctct gtgcctgcac catgcttgaa agctcttcta caggagctgg gtttgttcaa 139920gaaagcttct gtttctccca tctgcttctt gtaccttcac agggacagag ttagaagggt 139980acagccatgg ctggaagggg ctgactttca aatgtgccta attttccttt ggttgctgct 140040gcagctgcag aagaaggggt tcagaagcca agagctttga gataaggatg cctaacctat 140100gttgaagaca tttgtgctga cacctcaggc cccaggatag gacaactgct ggattgtggc 140160taacccacta gctacagaac ctaatttata ttaccagatt aggaagagca aaagaacatg 140220tatttataac aggaggtctt ctgtgcttct ctactaaaag gtgctgtgaa ggagcccaca 140280gtgcagcagt gtatgaggcc tgaaagaggc cgcagcacac gaagagccct ggtaggagca 140340gcacacagag gggcaggagg gctgggggaa ctgccaccca tggggacctg tgtgaagcag 140400tgcactcctg aggggtggac tgcgtgggaa aggaaaagaa agcaaacaga cctgtgatga 140460actgtcacac agactgcaga gtgacagagg agggcttgag gcagtgcgct tactgcaggg 140520agtggcgctc cttcctcaca gcagcgctaa cagcttggca ccaatattca gtagtctgtg 140580gtgatgcttt ttccagtttc accacacagc atttcgcttg ttctacttgt tttagctttc 140640cccctccaca agataacaca tactttgcca gtcagtccct aagaccttag cctaacagtt 140700agcaaacagg atcttgcaaa agaaggaaga taacatgaca ccaccttcac tggtgtataa 140760atagttcaaa tactttcctt cactttcccg taaattagtt gattgcaggt caggagataa 140820caggggaact tactgcaaga gagaaaatga tgtttaatat tgtcttggac tttctggtgg 140880tctgggcatg aaaatggagt actcaaaatc ctcaggacgt ttatttttca cctgatttat 140940tcccaaactg cactatttct aggccattgg agttcttatc aattaaatta tactttggct 141000ctctgctatc tcactccctt tcatcttcag catcactttc agcacaatta caggagaaga 141060cttagactca gagctttagg actcatcata agaggctttc attgctctgt caccacaccc 141120catatagatc tgtagtatac cacacatgtg aagaagcaca gtacattagt gcattacaga 141180gagacaaaac cacacctatt tgtgtgcctg cagtcttaca ccagcaggaa gataattaac 141240gtaatgaatt tctataaaaa tgagagaata tggcccctgg gtcctactgc ttgttctagt 141300cctgattctt caaacgtaag aatgcaagta aaattactca cttgaacaaa gtcagcaatt 141360tgcaagaact gatattctga agttcaagta attagagtga tttccagtac ttctggctgg 141420aacgggcagc tgaaaatcac ctggtccagc accttgctca aagcaggact atcttcaaag 141480ccatatcaga tagctccaga ccttccctag tcaagtgttg cctatctgca tggttggaga 141540acccacagcc ttctgattaa tttgatttta aacataaatt caaatgtcac tagcgtagca 141600gtagtgaaag ccattcaact ggctttactt tctcttacca aatgagagtt agctgcaggt 141660gaaaataagc cctgccagtt ctcatttttt ctcccacagc ccacaaagct ctcactgtct 141720gtcctcactt gtaatacttt tgaaccaaca tctacagatt atctctgtaa atcccaagca 141780gtacctagtc accacgtgaa caacaaattc ctacatttaa caatatttaa gagcaaaggc 141840cagaccatat gtagctgcac actacacatt tttagaccca atagtataat ttatactttg 141900actccatgtt gctgccatgt ggataacaat gcgcaatcat ttgtacctgg cttccttttc 141960taactagtat actcttaaac gtcacaagat aaagactcta gttctgtata gtctagctga 142020cttgtgacaa gagcaaacac tcacaatttc atggtactcc tgaggaaaaa aaggatccca 142080aactaatttt gagcttttac atattttttt ttaacctaca gagcaccttg ctacttctgc 142140tgaatgttag caatagcaac ccacagtctg aaatcaatgc aatgaacttc tactatgggt 142200accatactga tgacaggaat agtgcaagtc cttacactgg aaggctgact ccttagtcac 142260ataggtaaaa tttagaaatt gcagctctga taagagatca gtatgggaaa gggaaaataa 142320tggggtgcca gatgagtgca ccttcctgaa aggaaggcag atatatggga attaaaggtg 142380gacaagggat gctgtggagg taccatcaac tttcacaggg ctgtatgtaa aagcagctct 142440ctttcctgtt gattctccgc tgcctcattt cttctgggca aagtttgtta ctctccagta 142500acgtcccttc ctcaaactgt tacctaatcc caccctcatt gccttctctg ttttgctctg 142560tccttcagca gtctctacct gcttcttaag gtagtgaagt aagagggcag ttctggagtc 142620aagctctgtt tctatgaggg taaaggccag ggagagaaag gtttgggagt gtgaggagag 142680cctttttcct gtgttgttca agtacttagt ccaagctgct ttcagctgca tctgcagaag 142740atggggaatg gagggtgatc aatgccattc ctccagccac agagcaaggg ctttgcctct 142800ccttgcatac agtatactag ctttccttag tcaaatgttt cctctgtgct gcagagtcca 142860aggtaaagag gctttgtcta cagctaggtc tatgttccta gagaaacaat tagcaactgc 142920aaaatcaaga ggtactaaga aagcctctga agctataccc aggggtctgg caaatgaagg 142980gggacagatc aagaagaaag aagagtctag agcagtttaa gggaataatg ccactagttt 143040taagccacac atctggtggt aagcttttaa ctttgaaaga gacagaaatc tcaagataca 143100ccagcccaaa atataatgga gccataaagg tctgcacgta gctgaatccc aactggaaag 143160aacagcttca aagagcttgg aagtgctgag gtgaagaaga gcatgtgatc attagatttc 143220aaaagaaggt cctcagcaca ataaccagaa agttcacctt tctgtgggac aaaagatgcg 143280tccctcacaa aggctggggg aacaaaatct ttgcatctca ttttgcctga gaggagaagg 143340aaatacaaga tcatcttgtt ttacttggtg tgtatcacat cattaatttc tatttggtca 143400ctactatgca gaacttgcta acttgaacca tgtaaaaagc acactaggtc tcaagagact 143460aaaatgcttc ttgcaacagg cagagtgtga gagatggaag gatggaaaaa tcttgcagtg 143520atgaaggcac tgataagaga tgttgaaatg atactaacaa atggcactct atctttccca 143580agatctttgt cagcatgaag ggaaaattct attccaagct ctctttgagg ggttaccatg 143640ttccaggata aagacttgct gcatacacaa gcgcacttag tcaggtcact cagatcagtc 143700tcatgctaaa aagtgtgaaa atagaaatac aaataagggg ccaagcagat tactgaacag 143760caaagattgc cagtacgtgt ccacaatgag tatttggaca tttcactgcc gaaacttctg 143820aaaatatcaa ctgccttatg aaactctggt tattccaccg cacaggagta tttgtggttg 143880agctgcatga agaaatagca agtgtttaaa ctgatttctt aaaagagagc ctttcctcta 143940catgctgctc ttgcacatcc atgcgtggct cctcttcagg agcaggaatt ggttttctga 144000ttcagcagtt gtgtagctga cgtagttata ccctttgaga gatttcttca gaaaaatgac 144060atgtttaggc taaagtgcat gtaatccaca catacaccat tactcacaat gaagtactat 144120gcagcatgaa attcaggcta ttcttcttca tatttttggt tttaattgct accttggtta 144180cttaaaaaat gctcaccatc tgattcatgc aaaggaaaac tgcacactgg tagatgtgag 144240aacagcacgc atactcactt ccagataaac taatctctac tcagatatcg agatcattgc 144300ttctccagaa gtgttgcact ggtcatcaga actgagtatc tcaggaaaag cactgtcttt 144360tctaattacg gcatctaagc taaagcacac agcggtaata gtgcagtatg acaaattatg 144420ccagtgttca attcatgtgc caaatctcac cacgcctttg cgttctgcag gtgtggagca 144480aaatgcctca gtgatattta gacaggaaca ccaccacacc tcttaacaac tcataaattc 144540taaatgctat tggagtatgt cagcaaagat tgcttggcaa aggttgcaaa tgtacatgta 144600atatgtacgc tttagatagc tatctacact gtttcaaaat aaagacgcgt gtgttctcac 144660tcaaagcttt aaagggaaat aagatactca aagaaataat ctcttttgaa ctttaaaagc 144720tatttgagac ttcacgatga tacaaactta tcccacataa aaatcttagg acataaaatc 144780cattacaacc attccagctg agacatatac accattgtta cgctttaatt tacaaggtca 144840ggacaagctc ttgctgcatt ctgtgacaaa agggctcctt tgcacaccaa aatccatgca 144900cccactccaa gcacctgatc actgatcacc attaccatca cttcagtctc cgtgctccca 144960ttccccatac tgttttggct cttgccaatt acaggattgt tatgaaacta aatgttaagc 145020tgccctccca caggattcca acattctcag gtttcaaaac cattgtcttc cccacccctc 145080ttatctcctg aagtccttat aatggtttgg acatttaaag tcctttcatg tttaaaactt 145140actggcctgc tctggctgag acaaaaacac gagcagaatg ctctgttggc tgaaccagaa 145200accattcccc cccagataaa taaacagcac ttttactggt aaaaaaagat attagaagat 145260gccaaagaaa tggagtagct tttcttcaag cataattttt ttcttttcaa ataccaaaca 145320ccttaggttt gaattacatt agattttcaa gaattacaaa gggttcgtag ttaaaacagc 145380atacgtacat gaaaaccagc catggcaagt ttcacacaaa tactgtgtga aagcagaagc 145440taccaaacct tcctctcaaa accctcaagt acatttagat cactttataa atgatctatg 145500tagacagcaa gtatttaacc tactcctgat cccaggtacc aatgaactga gcaacatact 145560gtgtaggaaa gttgcactga cttgtgctaa gttgcacgga aactgaagga aaacaaaatg 145620tgcttatata gctgagatct ggccagggtg cctggtgtgc tgccaatatt tgtcctgcca 145680aaatggaaac atgaatgacc acagtgaatg aactacaggc ttacttccca caggaaggat 145740actaccaata caaacataag actttgagca tgttggagtg ttgacttagt agagagtggg 145800agtgagggaa ccgctgctcc tgagtcagcc tcagcaccgc ccattgaact ctgtacctcc 145860tagccttggt aacttcacag gatgctggaa aatattatca agtcatcaca ttgatttatt 145920gaatatcttc cttttagatt ttagttgctt tgtatgtatt tttttttcca ctaataacca 145980gccatgctat tcaaaaggca tttttaaaag gcaacgttaa caccctgtac aaacaccatc 146040ctctcattca ttcaaatccc acatttctgc atatatggaa catgtcagtc acttcttgta 146100acagagcaag tactatgact agtcagcaaa ttaaattcat ccctgcttta aaaacagaaa 146160atccaagtaa ctgctccaag ggatgaagtt tatttagtgt atctatcatt tgttctacaa 146220cacagttaat tttgcaaaga tgactcaaat catttaaagc tttggaaatc atttaaggcc 146280aatgtaaaca gattacaact ttcccaggcg caatggaagc aattaattct gcagcacacc 146340tctcctacac tactatactc tggaaaacgt aacagatgca tctaattata acccacactg 146400aaacatgctg tctttatgta gctatgaatt caaaacagct gaggggcagg aaagaaccat 146460cctcctaaag ctatgtggct gctcacctgt aggaaagcaa cttcagcaaa gctttgagtt 146520cccaggttac actgatgcag aagcttcacc actggcaagg tgctccttgt gtgagcaacc 146580tattctgctc tataaaacat ttagcagata atcccatatc tcagtcctca gtacaagaga 146640ctctgtgcca gccacttctg tacgaaataa gcccacacgt actttcatag acctcagggc 146700agaagaaaag tttcagaaag cagtttgtgc tgaggagata gaccttgggg gtgagtcttt 146760ctccatattg aggcggaatc cctcaaagac aagcagccct tatccaggtg ttcaaggtga 146820tattttcaac agagcaggga ggtaaagaat gaaataaagg gcagagttac ataggatttt 146880tcagtcagag gtgagagctg agatggacag gacaatgagg taaggacagt gtgactgtga 146940ggagaattag cgatggaaat gcttcactag ccaaggcaag aagaaaaaga gtattcaata 147000gaatatcaat ttctggggaa agaattcatc tctgaagggc tacatagggc aaatagctgc 147060tttcaactta gaacagggaa actgaggcag cagcaaaaaa aaaaaaaaaa gtctaatctg 147120aaacccacat caggttctac tgttgttgca gtgataagaa aagtgtctga tgagtgtttc 147180tcaaccttcg ttatctcaca gtgaaaacat tttcctggtt atacagtttt agaatcctcc 147240aatattacca aaaaatcatt ttactaaaaa tggaatccca caagaaatga ctaatttttt 147300atctgtagga aacggacaat agaaaaactc ataaatatga tgtcactgtc ctttcgctgt 147360ctcttccttg gaaattgttt ctattagagg aatcataagt aggtcagcta ctgcattttt 147420ttaccctcca aattgcaaaa gaaatgttgt ttccagcagt gatggttcaa gttgtaacta 147480gcctgttgcc acaaaaatgt ttatagaaat atttctgcag tcagttttgt aaggttcttg 147540tatggtatca ctctcaccgt cacttcacat cctactctga gatgattcag ttcttccata 147600gggatgtggc cttcagggca aaataaattg cagagtcatg agtcataaga acctttgaag 147660aacaggccag gcagactata aattcagcct acccatcatc tgaactgcat aaaccttgga 147720cagacccaga gcagcagtct tcctccctgc acaaaacaag gtactgtaat tatttctaga 147780gattatttat ttcacctact cttggatgat gtcgatctgt tgacaaatgc atagaaaaaa 147840aaggcagaag gaatctgaat agaaaacaat aaatacttga ggaagaaatt tactaagatg 147900gcacaggcaa ggtctagaag gggtaagtct cagaaatatg caggaagagt ggtttattct 147960atgtagttct cactggcaaa catgtatatc atagcagagt aagaaccatt gtgtttgctt 148020aagttagatc atttgttcat gtgctcttca attcttgtgt acgtcaagac acagtcagta 148080catctttatt ttatggctat tctgtatcaa ccagaatagc tcccactaca tacctagggc 148140tctcagcttc aactgcaatg caaataacaa agagcagcac ctgtgttcta ccaataagga 148200aatttgtctt gcagaactgg gaagctatga ttcggactag caccataaga cagagtttcc 148260agaaattttt gaaagttaaa aatggaattc aggatactta gcacacagta gttagagagc 148320tgcttgtcta gtgcttcaat attttcttgt atcctaaagg aatggaatat ttgttcacta 148380cagttacgag ctccaaaagg ctcttgcacc agtagtcata agaaacaatc tctcagcatc 148440ttctagccct cgcaccagtg agtagcatta tcatatgtca ctccaagatg ctgtaaagga 148500cagtgcaata aacgtcctga tgaacaaata caaaatgaaa tatgaggctg ctttttctat 148560atcacttgag tatggttagt gttgttggtg aaacgggatg cactatactt aatataactt 148620tttagtagct aatcttcctc attttccata aagatatctc tcttctttcc atctgtaagt 148680tccttaccat atcattatat tgcttacata gaattcacca tatagttata aattgcatgc 148740tttttctttt tagtataaca ctgtaaccat cctgtttcga tgaattttct tttgtttctc 148800ctggctgtcc cttgcatgga tatctgtctt tctaagtgaa cttctgctga accaaggaga 148860gttcattcat gcactacaaa cagacgtagt tggcagaaat gaatattgta ccagtactga 148920caggtcagaa tgctttcatt cagtttcctg aggtcagagg agagctgaag aaaatacctg 148980gcacagtcta gattttgcct ttgaacatac actgccagtg agcctcagtc atacaaagga 149040tcactgtgct gcactggcat tcttccacag tcaataagtg ttaaaaatac ttttagaaag 149100cctacaaatc ataaaataaa cttcaaatac tgaaagggag cctgcagaca tatagcaaac 149160acaattaatt cttagctaat aacatctttt gtccctttct gcgcaggttc agaacaatgg 149220aagctttaaa taaagcaaac acaagctttg ctcttgactt tttcaaacat gagtgtcagg 149280aagatgacaa caagaacatt ttgttctccc ctctcagtat ttcatctgcc ctggctactg 149340tgtatctggg agccaaaggc aacactgcag atcagatggc aaaggtgagt ctgagaagag 149400ttgatctact ggagtaacat tctctatgat agaaatttag catgatgcat caaaggaaaa 149460ccttatgcag gtcaaaagat acagtctaca gtagcttctg taagcaggcc cacaccaaat 149520gggagcagtg gcattagtga cattgtctcc ttttaaatgt cattggaaga aagaagagct 149580cttaatccca aagctcaata acttgagcac tcaccagtga gagggagact cggattctcc 149640acctgccttg ctccagaaaa ttctcatttt ctgtctcatc tctctggaaa tggccctatg 149700cagaaagacc ctccactgta tctctagtaa gttttgtgct ttgctaacat aaatctacaa 149760acccacaagg tcagagaaga aacacagtca gggtgataat acaacccctt cttttgagta 149820cgtactttac aagaaaaatc acctactgaa gttcctaaac tctgtgcaaa gttcatagct 149880tcaaaaggca gcaatgagaa cagccccagt gcaaacatgg ttacctagca tgtactgcgg 149940gaggggctga tgaactggca tctgctaagg cagaagaagc tgctccacct gctaaggcag 150000caatccaact actgagctac aggacaaatg aggaccagca gggtcacaaa gaaaggagag 150060atcttctgtc aggaagaagg gaaaacaaaa caaaacagaa ggcttttgaa aaatgttcca 150120aggttagatg tacacctctg tagcctgggt aaggtgcaca tgcccagagg aaggcattta 150180gggtatcaat ttgctcccag tgtttacctg ctttctgaca tgtaccaggc tctccatttt 150240acacccatgc tttggcagtt tccacctgca gataactggc ccgtcccagg tattacccta 150300tgagtacaag agccgatttg aagcaggcaa gttcctctag aaataccaga tatatgagaa 150360ttctgcttgc agccctcatc ttagtgtgct caagacatcc tgtacacatg ggctcaaaag 150420taaaatctgt ctttgtctct cttcatcacc agtcccatga cattactgaa agtttttact 150480gaaacagcaa attttctatc attgcattta ttactgcaat ttccactgca ggtactctac 150540ttcaacgaag ctgaaggagc cagaaacgtc accacaacca

taagaatgca agtctattcc 150600agaacagatg agcgcctatc aaatcaccgt gcctgtttcc agaaggtata taaccaagtc 150660taatgatcat agaatcatag aatggcctgg gttgaaaagg accacaatga tcatctagtt 150720tcaatccccc tgctatgtgc agggtcacca accactagac caggctgtcc agagccacat 150780ccagcctggc cttgaatgcc tccagggatg gggcatccac aacctctttg ggcaacctgt 150840tccagtgcct catgatcaag ctacagtcat gctaacacct tcccttgctt ttattttctc 150900tctctgtttg ccttcctcaa atgcagggta cacaactgat tagtacagca tcctgtgata 150960ccttcacctt atgcaatact taagacatgc ttcccatttg taggtagaat tgcaaaattt 151020aacctcaaat ttgcaaaatt tgaaatttaa tgatgggact attctctatc agtggacctc 151080ttgatcttct cccttcagct ttgagatttc tctctttttt ttttctcttt ctttttttcc 151140cctttcctct tctttctttc ttctttttct ttttctttct ttctctcttt caatctttca 151200ttccttcatt cttttctttt taactactac tgttcaatta gttattgcta gttattcagg 151260aacatgttct ttgaacaggc acagtcccta tctcagaaca aatcagaaca acaaaactac 151320tctcccacac attggattgc agcatcaaca caacaacaac aacaacagaa aaaaacagcc 151380agagaagtaa cttttacaga ctatccatgc ttgatacatt tcagaaatta gtttctattt 151440catttgaaag tttagtggaa taaattggca tgttggaatt tcctaaggta gaccttgcaa 151500taaatcttaa agaaatggga attattgtat tcccgagata tttctttgac agactggcag 151560gcatcttttg ttaaaataga cataatttaa gagagcagaa aatttggaag tcaaactctg 151620agtgagtaag gtagtttttc ctcactgaca atgcagccac tgtggtaaaa agttcctctc 151680cctactcttt cccatcattc ttttttcttt ttgtgagtaa atcatttccc tgaagtctgt 151740ccacaaaacc cctgtggcag caagttttga taaatgggaa cttgggtcta cattccacag 151800ctacggtggg aagactaatt ttggggacta cgccaacaaa ccatttatgt tgcacgaaca 151860ggagatggat tgtttctcat gagtaatgct tgtctgaact gtaagaatta tggagcgctc 151920taggcaggga aaagaaactg ttctaatagc ttagaaattt agatagctgt tcatgcttct 151980gattttcttg cagtaacaag atgaatacaa cacaggtcca gtttcttagt ccactaattc 152040acagcttcat ttccttaagc tggtttgaca gtttgagtcc acattcatat aattctgtta 152100cataaatata aagaatttac tgcaattact acaacaaaaa gcatttgcaa aatattatta 152160tttagaggta ggttaaaaaa gttagaggca aacttaccat gtaattaact ttcataaatc 152220ttatcaggag tcacacagcc aggtcttcat gtatagttta gcaattacat tctgtctctc 152280tctctgtatg tacttcattt tgcaacctcc atttaaaagt ccttaaacat tctaaacagt 152340tcaagctttt actacttgca tcccagggct cttacagtgt ctatagcata tctgaaactt 152400ttagtaattt cacatcattc ttttaatatc tgtctgagtt agtacacatc ttgcattgca 152460gtaaaggcaa caccacctga atagcagtag tttacataga gctgcatgag gaaagaattt 152520agaaattttg aactgtttta cagaaaaaaa aaaaatgtat aacccttatt tccttgtctc 152580caagacagaa ataggcaaat caggtaatat ccatgctggg tttaaagcac tcaacttgga 152640aatcaaccaa cccactaaaa gttacttgct tagaagcgtc aaccagttat atggagaaaa 152700gtcactgcct ttcagtaagg taggtaggcc atttattcat gttatcctgt gtgtgtcaga 152760ctttatgatc tatctatgac aacaaaccat aaattatatg ctttcaaata ttttcattac 152820atctgcaaat tgtgtaatta tctttaacat acttcctgtg aggttcttct tgagaattta 152880gatatcatga cttttatagg atgtatattt aatttgtgtg attcacagtt gtggctacgc 152940aaaaacattt aaattatgta tttccaaata aaatcaatac tatgttcttt tgacaatgct 153000gtgcttgtag cctacacaat ttttatgcat tctctccaat cggctatagt tatttattgg 153060cattcacact ggcaggcaac aaacataaga cagatgtcta tcttgcactg caggaatact 153120tacagttaac caagaaatac tacagtgcag aaccacaatc agttgacttt gtgggagcag 153180caaatgcaat cagaagagag atcaattcca cggttgaaca ccagactgaa ggtaagctct 153240agcatctcct ctcccagttc tgaaggaagc agttttagtc ttgaacaatt tctctgtgcc 153300caaaggcagg taaacaattt aactcagaaa ggaaaatcag aacagttttg ctgaagtaat 153360catctgctgg caagcccttt ctagaattat ctttcaccat ttgaaaggga gaggaatgtg 153420gtttcctcta taaatcaagg ttgtcatgta tttatgaata atctcaagct agaagtatgc 153480caaatcagca ctctaaattt ccttgtctta tgacttcaga aactacgcca gcatttactc 153540tgaaacagta aagctgcaca aatatgtaaa cgttctttgt ttttctctag gtaaaataaa 153600aagtctgctg cctcctggat ccatagattc actcaccagg ctagtcctgg taaatgcgct 153660ctatttcaaa ggaaactggg caacaaagtt tgatgctgaa gataccaggc aaaggccttt 153720cagaataaat acggtatggt aacatactgc cttatatacc agactgcagg ttgaaaaagc 153780agtgaaaaag atggaggaga taaattcctg tcattcttta aagccacata gcactaaaat 153840tagtatattt aaaacatacg ttatatcctt cttagcacat cttcagtaca aagaccgcat 153900acatatgcta gcacccaagg cacaaataaa attatcagaa gccagcttga aacaaacttc 153960catacacctc ttaaagcagg aaaaacatag atgtgaatag aactgtatga actagttcta 154020tatattttca tttttaacca tacaatgaat tggagtggaa cagagcttcc agtaaatacg 154080tgtcatccta gctggctaag ataaccttcc cagcctccca gtgcattccc agaagagagg 154140ggccctctgt agatcctaca gcttctctta gagccacagg gatgtacctc catgctactt 154200caatgtagtc tttactgttc tgagtataaa tagcaagctt ttcatttgat ttgttgcagc 154260atacaactaa accagtgcca ataatgcacc tgagtgataa atttaattgg acctacatag 154320aatcagccca gattgatgtt cttgagcttc catatgtcaa taatgaactc agtatgttca 154380tcctgctacc acgggatatc actggcctac aaaaggtaaa gggtaacttt aaactcaaat 154440tgcgtgagaa acaacgtttt catgcatatc catggcaaag caatcctgtt tctaggaagg 154500aaggtatcga taaggctaaa ggaaaaacaa accccaaact tgcccaaatg ttatgaagct 154560gaaccttttc aatgttttgt ttggttttct ttttaactcc tggcacgtgg cacctcgtgc 154620ttcctcatgt tgatcagtgc tggaaataag tagcccgaat ccaacaagat agatctaatt 154680ccagctgaag aacaacgagg acagaaagat agttctgctg actgtctgta ctgattcgga 154740cagatattat tacattaaaa agaaaagcac aaactggaca ccctccacta ctttctgtga 154800tgtttagagc taatatacat gtacactgcc accttctgta aacacactga acctgacttc 154860agatagtgaa ctactgtgaa attctcattt acattagtgg gtgttttgta gaaaaaaaaa 154920aaaaaagtta ttttcactaa attctaagac acacagaaaa cagaaatgtg agcagcaagt 154980caaatagact attgttactt gacagtgacg ttgttttaca aatatttaat ccctctatat 155040tccctgatga ttactaagaa cagttcaaat actgcactaa catgctgtag agcaaaacac 155100tccttcctag tagaaaatat ttcagagttg gcatttcact aatggtttct gtacttgaaa 155160agtacaattt tttttgctac aaaaaaaagc tacagaattt ttgtagtttg aaaagttctt 155220aaataagaat ataaaagaaa taacccctag ggaacagttt tttgaacact ctgtaatttt 155280ctggttctct tttcaattaa ctgcagctaa taaatgaatt gactttcgaa aaattgtctg 155340catggaccag tccagaatta atggagaaaa tgaaaatgga agtgtatctg cccaggttca 155400cagtagaaga gaaatacgac ctgaaatcta ctttgagcaa gatgggaata gaagacgctt 155460tcactgaagg tcaagctgat ttcaggggaa tgtcagagaa cgctgacctg tttttgtcac 155520aggtttttca caagtgttat gtggaagtca atgaagaagg cacagaggca gcggctgcca 155580gttcagcatc tctagcgtca cgaacccttg gtgctacagt tatttttgta gcagatcacc 155640cttttctctt cattatcaga cacaacaaga ccaagtgcat ccttttcttg ggaaggttct 155700gctcccccta gaaaatcagc tattaataaa caagccctta caacaacgat gaacacaatg 155760tatgccatga agaacacctt gacagacttt gcactttacc attttcctgt actattgaca 155820atctctttta gaagagagct caaattaaaa acatgaattc aaacctctga ttccttttcc 155880tctgcaaaga atcctagcat cgtatactgc actgtagaac actgaactgc acgctgaaca 155940acatggatgt gtcttttcag tgctgtccaa accagaactg ctacaatgca gaacagacta 156000ggctgatcta aacagtacct tctgacccag ttcctttcac acgtaagaag aaaagaaaca 156060ggagaaactc attcctgcat acagctgttt catctcttca aagccagctg tcccaggcca 156120gctcaatcac agccttgtca gttttaaatc agcttcacaa catagcatgg ctggtaatga 156180aacaaaagtg caaaatcctc tgtgttgctg atactggtgg tttgctcttg cacacaaagg 156240agctaacaca tgtactttct aatctctgtc cctcataaac tagcaaatac caaacaatac 156300agaaccagag taaagtaaaa tacatacctt gaaatgcttt cttttgtcat aacctttaat 156360tcattcaacg ctgttgcagc ccagcactgc actgctttac ttgcctttta ctttgccaca 156420tattttgctg cttggagcaa gtgggagaat aaagtctgtt atgttaactc cctaagtgct 156480gtctaaaaga ttacatgcaa attctcctct acatattcac tgctttcaca gcttttactc 156540ctaaagggga ggaattccta atcagtcatg cacatctaag aacacaggtg atgctcctgt 156600ttctctgaat tcagaacagg gaggaaagga ctgggtctct taacagcact tgcacacaca 156660ctgacagcat ctcactagaa acatcccttc ccagaaaggt aggatacctt tttcctggca 156720gagggaagag cgctgactga tagtgagtcc tttctgtatt attccacgtg accaactgtg 156780gccaggctcc cttttggctc tgcttcccaa atgggaagga acgtagggaa gggccaatgg 156840caaccaaatt agagggtgag tcttgcattg aggaacacca ttttcccacc gtaagtagca 156900cagcactggg gcagactgcc cagaaaaaaa ttgaggattt cccattcttc aaagggctgt 156960agctgaccta atttaccagt gggtctgctc caggcatgag ctggactatg gaaataccca 157020taaaccagct tgtgtcttgt ttctatcaac atccattcta ccttaccacc tcaatttctc 157080atcctctctg gcacccttac agcttgacaa gcaggggcag ttagcttgct tgcttgctct 157140caagcatatt tctttgagac ttggaatctc cctagcttgt actttcccat caatcaatca 157200ctcagtggac ctttcttcta ccctgatttt ttgacaactc ctctcattgt actaaagtca 157260ccacttgttt agtttctggc atttcttcat tgccttgcat aatactgatt tctataactt 157320ttcagaagac ttgacccgct ctgttcttgt aatcaagctt acagcaaaat gcttggcaca 157380ccatacagtt tccttccctt cctcctcctt ccttccctgt cccctagact cttacagata 157440ttgccactac cttccccttc tcttaccacc aaagagctgc tctgctgtct tctgggacag 157500cagagtgaca actattgtga catatttatc cctgttgtat tgttttcact ccctccttgc 157560tcaagctctg ccagtggggc cgtttcagtt cttgcccacc tccatggcat gcaagtactg 157620cacactaact cagtactctg cagtgcttct ctgaagcgtt tcccagccat ggatgcaatg 157680agattctttc accaacacaa gaagcaaggt attcaacctt acaccttcaa tggctttgcc 157740tctgcctatg ctcctaatga tcttcactct gaagcttgac tctcagagtc tcattcagtc 157800agtaagctcc tgacatttta tgctgatgca tcttactcat agaatcatag aatggcttgg 157860gttagaagat catctagtcc caagcctcct gccaaccact aaatcaggca ctacatcagg 157920ctgcccaggg ccccatccag cctggcctcg aacatctcca gatctccagg gatggggcat 157980ccacagcttc tctggggaga ggttccagca cttcaccacc ttctcagtga aaactttcct 158040caacatctaa tctaactcta ccttatttta gttttaaaac attccccctt aacctatcac 158100taccttccca tgtgaaaagt tgatttccct ccttacttac aagcttcttc taggtacttg 158160aaggctgcta tcaggtctcc cctgatcctt cacttcttca gactgagcaa gccaagcttc 158220ctcagccgat cttcagagaa gaggtcttcc agttctctaa ccatcttcat ggccctgctc 158280tggacttgtt ccaaaggtcc acatctttcc tgtgctgggt gcctcagacc tggacacagt 158340actccagatg gggcctcatt tgggcagaac agagggggga caatcacctc cctctccctg 158400ctggtcaccc ttcttttgat gcagcccggg atattgttgg cctttcaggc ttcaagagca 158460cgctgctggc tcatgttaag ttcttcatct gccaggatcc ctaagtcctt ctcagtaggg 158520ctactctcga tgaattctta tcctagtatg gatacatatc tgggattgtc tcaacacaag 158580tgcaacacct agcacttggc cttgttgaac ctcattatgt tcacatggac ccaatcctca 158640agcctgccca ggtcgctatt aaaaaaaaca agtgtcatgg cttcacaagg ctggaaagtt 158700ggatcaggcc tacaatacct gctaacatcc agaaaccaaa accatgcatt ctggctctgt 158760aatcatttta ctagatttat ttagtttaaa caacaggcca gttgtcttca caacaacaga 158820agatcactga aatgagtgag tgacttgttt tactgtcctc tcagtagaca attgtggtgt 158880aacagttaaa atgagattat gatccacatt gttcctttga aacgcctaaa actaaaacat 158940aacatgatga acaaggaaga caaaactgta tgagatcttt ttgtgattca ttagaagctt 159000tgagtaggcg ggaacagtgt tgacatagga gagaaggaaa aggaagtgca aactgtacta 159060tatttctaaa tttattcact gcataacaca caggcacaga acctgactga ggacaagact 159120caggtcttct ctctcacggg accacaggta aacatttaaa ccaatttaaa taactttttt 159180gatgttttta aatggttatc tatagcttgt atgacaatgt aagtatatta aaacacacca 159240gagttattct gtagtcggga gcataattga tcacaagaag gaaaatcttg tcaggacagt 159300agctgtctta ctaattaaaa tgttcagttt gataaaggag tctcatactt caggtaaagc 159360aaaggccatt ttcattctgc cttgtatgag gtcaggccag ggactcagag gagcagagta 159420aaacagacag atttctatcc tgatgctcat ttggtcaggt tctccaagga gaggaagtca 159480ctctgttggt acagatttgg tgtagactgg ataacgactg ccagaaaaac tgaagtggtt 159540tggtaaccaa aatcttgata aatatctgtg gacttcagag attgtcctgc aatttctgca 159600gtgccattca ataaatataa atctttcttt acataataat aactactaca acaacaacat 159660tttccagtcc ctctatcaga aaacaacatc aagaaggcac tactgaacag gtaagttaaa 159720gtttggaatg ctcatagctt atatgcatag gtatttgcca gtttctgggg aaaataaaat 159780tgcaagaata taaagaagag attgtagtta gacttcgtga ataaaatggt aacactctaa 159840aagcaaataa caactttgcc atacattata ttatctgaaa tgggtgacta gccagaaaaa 159900ttccataagc ctaagagtta cacctaaata cattctcagt atcagctcct aattctatct 159960agatccaaaa tgaggtagtg aaaagttcaa atgtcccatg tacaaaaaac tacttaaact 160020tccctaggaa cattactttg ataatgagtt aagaatgaaa atgaacaaaa tatgcagctt 160080acaaatccac acacttttga aaaccaaagg cagaaagaaa cacaaataaa agggcagatc 160140tataaaagag gacatatcta taatcataga gaaatatgag atggataaca aaaacctaaa 160200agaaactgct gctcccagca ggtggcacat ggtatgtgta gaacatataa cgtacaacta 160260ggctattagt ttcaaaaggt acctacgtgc tccgttgcaa atgtaacatg taaatgtaaa 160320atgtaaatgc aaatgtaact aatatgcact acatacatca ttttagacac tcaaatacta 160380caattctgtc tgttgcctct ttccaggctg tagcaatgga acaggtctcg gcatcaattg 160440gcaactttac agttgatctt ttcaacaagc tgaatgagac caacagggac aaaaacattt 160500tcttttcccc ttggagcata tcatctgctc tcgccctgac atatctggct gcaaaaggca 160560gtacagcaag agagatggca gaggtaagta gctctgtgaa gctatgatgc tcaacactgc 160620ccagcactgc tgttgagatg ccctgctccg ttgtcatagg gaaaaactac atttgagttt 160680gcacaaatgc attgctattg ctgagtgcaa tggctgtgga agggatttca gccttgtagt 160740gcacagacag aagcactgtg atgatgctca caggcaggag caatactatt ccttgttact 160800gtaggggatt tacatatact agagctccag tgtccctctg attagatcag aagatagcac 160860agtgtgttat cataaggatc caaacaagac aaccatttta tctcttttag gttttaggtc 160920atgcaaactc tttcatgtca gtttcttacc tttggaaacc ctgtttgcag gttcttcatt 160980tcactgaagc tgtgcgagct gaaagctctt ctgtggccag accttctcgg gggagaccaa 161040aaagaagaag aatggtatct attaaacatg aaatcccaag ataagagttc aaatgtctgg 161100atatagtttt taagagtcca ccatttcttg tttgcagctc tctttatgtt taaagtataa 161160aacccaatat acttcgcatc acatccaatt tcagttccct tcactcattc agactcaaaa 161220gtatagaagc acaagtcact ggtataatct gaaaggattg caatatggta aatcagttaa 161280tcaaatcata aaaggagccc tgcaaactgc agtggtggtg aatttggaaa gataaaaagt 161340aagagagagg aacagaaatt cttcccccac atctacccct tagcgtttca aaaacttcat 161400gccaaaaatg caactggtaa atgtacagtt tctctttcca aggaccctga gcatgagcaa 161460gctgaaaaca tccactctgg attcaaagag ctcctgacag ccttcaacaa acccagaaac 161520aactactcgc tgagaagtgc caaccgtatc tatgtggaaa aaacctacgc attgctgcct 161580gtaagttgaa tggttttatg tcaaagaaga aaaagaaaaa aaaagaaaag aaaagaaaag 161640aaaaaaagaa aaaaaagttt tgcattgtat tcacttacca ttaatagaac agatctgaag 161700ctgtccataa atgctgcaaa tgatgagtct tggcttccag tgataaactt cattggaaaa 161760tacaatttgg tcttctccca gtatataaga atgcacttgg ctgtaatgca ggactccttt 161820tcatgtaata cagctttatc actaggaacc tcagtacata caattgaaaa tgagatatta 161880aaatacacat atccagggga tttgcacagt cttccttcct tctccaaata aaaatgggaa 161940cgagagaata agagtatttc ctttggttat ttcctaacca ttaactcact gctcaataga 162000gagcaaaatg ctagatcctg caattgcctg tgtgcaaaaa gttaacaaga agtccagtag 162060ctaacaaatt actttttgga ctataaaaat actgtacaat acagaatgtt tccttctttc 162120gttctttttg tatgccattt tccagacata tctacagctc agtaagaaat actataaggc 162180agagccacag aaggttaact ttaagacagc accggaacaa tccagaaagg aaatcaacac 162240ctgggtggaa aaacaaaccg agagtaagtt gagctcaact ccaacatcct tcctcttccc 162300actgttccct tcgggaccct gttcccactc ctgtgactgt ggcatccagg tcatgccctc 162360tggtgtgggc agtagatggc tgtctgcttc cagctgcttg ccttgagact gtggcgtttt 162420ttcaggcagg agccaattgc tgtcagctag ccaggagaac tgggcaacaa acagcaaaca 162480gactaactgg tttatgtcag ggaagtaatc cagggagtag ggcactgagg cttgcacttt 162540ttcactaagg agttgaactg agtggataaa gaatcaacac attccctcac tgtgttacac 162600tggagtaaag cctgactttt ctgatttcaa aaggtaaaat aaagaatttg ctgagttcgg 162660atgatgtgaa agccaccact aggttgatct tggtcaatgc catttacttc aaggcagaat 162720gggaagtgaa atttcaggca gaaaaaacat ctatacaacc cttccgactg agcaaggtaa 162780gctcctctgg tgtcctcctt aaaacaagca gactggagac tgcacccact accatctttt 162840atttcatcca tcctttaggc attccttggt aaacagactc tctgaaaagt tgtttacagc 162900aaaacatgtc agttgtcagc tcaccaacat ttatggaaca ttaagatgct gctcaggcaa 162960aggataacta gatccagatg gaacacagtt tccaaaaatg ctagggtcaa ttaaagcctt 163020tttgcaagac tgaggtataa gagctacatt gtaaaaatca gatattaaga gtccatcctt 163080cctgcacagg aactacatgc tatgctatgg acgagtgcag tacccgcgcc tctgtgctgc 163140acaatccggc tgtgaataca gctgctaaag tatggatgca gcagcacagc tccactggat 163200gggtgcatgg ccgagtgaga ctagaagtaa tgttgccaga gaggagatca caaaaaggct 163260gcacaacatt tatcctctca caccatagct gtttcattgc tgtaatgttg ggtgcctgta 163320tgccatgaat gctccatccc cctaattctt gaagatattt ctgactccct tcctctctcc 163380tctgtgggtt gatgtgcatg ttctggggaa aagagaacat cagttagctc agtccccagc 163440aaaatactct gggaaaagag ccaagatcag caatattgtc cagtcaagaa aagccttgga 163500aaaagaatgt caaatctctg ttacaaaagc tgcttatgaa agtttcctct ttacaaggaa 163560ttcctttttt caaggaataa ttttaaccga taaataaata ccttacagaa caagtccaag 163620cccgtgaaga tgatgtatat gagagataca tttccagttc ttatcatgga aaaaatgaac 163680ttcaaaatga ttgagcttcc atacgtgaaa cgtgaactca gtatgttcat cctacttcct 163740gacgacatca aagatggtac tacgggtctt gagcaggtaa aaagttctgc tacatccatt 163800ctgtatcgcc actcagtcat cagaacaaaa aggacaggct gatgaccata cggccctttc 163860tttctttggc agttcattcg gcagaagtag cgcacaaaaa cttgcagcat tatgtctcac 163920atttgctttg cagcctgttc tctggtcatc agtaaaagca atttatattt catattttca 163980gctgaatgtt aaatacgcca tttaaaaatc tgtttaaatc attaaaaaaa aaaagacaat 164040cataattaat tggtttatcc ttgcaattat caaattcctc tcatttctta aacaacagct 164100ggaaagagaa ctcacctacg agaggctgtc agaatgggct gattcaaaga tgatgacaga 164160aactcttgtg gatctgcacc tgcctaagtt ctcactggag gacagaattg acctccgtga 164220tactctgaga aacatgggaa tgacaactgc cttcacaacc aatgctgatt tcagagggat 164280gactgataag aaggatttgg ctatttccaa agtcattcac cagtcttttg ttgcagttga 164340tgagaaaggc actgaggcag ctgctgctac agctgtaatt atatcattca caacttcagt 164400tatcaatcat gttctgaaat ttaaggttga tcaccctttc cacttcttca tcagacataa 164460caaatccaaa acaatcctgt tttttggcag attctgctgc ccagtagaat aaattattcc 164520tcactcctag agggatccaa agttcacttt tcaaaggaaa aaatgtgaac tgtagtatta 164580aaagctcagc cttcaatcat atagccataa gtactggaag tctatgtctt tttccttaag 164640taaggcagca cccagacacc accacgcgcc tcgaagactg tctctctact gctcctttcc 164700attatgctca tgaaattgcc ttttatagaa agcaaatgct tgaggtacaa ttgctagcct 164760ctgttcacct tgcgttttgt ccttatttct ctaaactctc aagactgagg ttgataagta 164820tcccaaccag caaaaaagac caagaaaact acaacaatgt gccttattgc tacctcttac 164880tgaaatgtga cctaaacaat tcaaatctgc ttcccgtttt cattaacata attatatgtt 164940tcctggctaa catctgcacg gtctccttgc tacctggatc attgataagt gtatgatttg 165000taacttacga gtgcctttca gctaagatag tcccggtatt gacagaaaca ccagtaacat 165060ttttatggat gcttcacttc attattttgc catgatctac atttaaacaa taaatgaatt 165120tggaactgtg tttatgctat gcaagattct gactcacgta gctcttttac agcatcctgt 165180ataatgggtg gctgacacat atttccattc ttgttatttc aaaccaacca tcacatcacc 165240gctaacgaca aagtgctgag gcactctaat aaaccagggt cttactccca ctagatttca 165300tacaacactg aaaacactgt cgttcaacgt gttatcgtag acatatacta gacacaccaa 165360ttcaaatcaa agcctgtgat aacagagtta aggcatttgc ccagtcttgt tcaacagctt 165420caccaatagt ctagataaag ggatagagtg aatcctcaac aagtttgctg acaatatgaa 165480gctgggagga gtggttgata caccagaagg ctgtgctgcc actcactgag acccagacag 165540gttgtagagt tgggtggata gaaacccaat gagcttctac aagaacaagc atagggtgct 165600gcagctgggg aggaataact gcatgcatca ctacagatca

gaggctagcc tgctggaaaa 165660aaacctctga agagaataag ctgggtgtct tggtagacaa cagattggcc atgagccaac 165720agtgtgccca tgtggcccag gtggccaatg gtatcctggg gtgaattaat aagagcgtgg 165780ccagcaagtt gagagaggtg attctcccca tctactctgc actggtgagg ccacatctgg 165840agtactatgt ccagttctgg gctcctcagt taaaggaaga cagggaactg ctgaggagag 165900tccagtggag gactgccaag ttgattaggg acctggagca tctcccatac aaagaaaggt 165960tgagagaccg cagactattc agctcagaga agaaaagact gaggggtgga tctcatcatt 166020gtttataaat atctaaagta caggagtcaa atgaatggac ccagactgtt ttcagtggtg 166080tgcagcacaa gggggcaatg gctaaaaact gcagtgcaga acgttctata caaacatgag 166140gaagtacttc tttattttga gagtgacaga gcactggaac aggcagccca gagaggttgt 166200ggagtctcct tctctagaga tatttaagat ctgcctggat gctttcctgt gcaacctgcc 166260gtagggaact gctttagcag gggttggact ggatgatctc caagaggtcc cttccaactt 166320ctatgattct gtgatctgaa cttgctttca ctgtaagcat tcagtttccc attgttgacc 166380actgcttatt gcactatcag cgccccatgg acctactgag aaagctcatt ttcttctgca 166440ttatgataca caagaaataa aagtctgacc agagcaaata gcccagaaga gtggcttcca 166500aacatccaat gctgtatcag caacatccat ttcttccatt agcttaggag ggcagtagct 166560gtgctatctt atgcctataa ggaattggat aaagttttgt agccattatg atgcccacct 166620accacacgtc acaggacatt atttcaagca acaagatctg aatgctggag ggaagtactt 166680tagaaatata tactcctagc aaaattccac ctgtaactac agctcactct atgcctcaac 166740aaaaggtaga ctatttggaa atatatctcc acccaacaaa gtatatatac gccgccttct 166800ctgtacactg tgctgcttct aaactcagtg ccctccctca cctgtaaccc ccagctcagg 166860gttcagctgt ctgtcactga ggacccactt tggttcagcc cagctgcatg ctactgactc 166920agctttgcaa agaattgcac attgtaaatt gtataagtgt ggaaaagcac acaagtgaaa 166980actacacaat ataagtaaag taaaaacaca aagaagttac tgtcaggatg gaatagtcag 167040catagcaagg actcgagagg attgcacgta ggggtgtcaa acttcaatta aaaaaaagtt 167100aagaattgga attctccagc ttgttcaggt ccttctgaat ggcagcaaaa ccctctggtg 167160tatcatccac ccctccaaat tttgtattgt ctgcaaattt ggtgaaggtg aactctatct 167220cagcatcctg gtcattaatg aatgtgtaaa tagtactgac actggtgtta accgctggag 167280tacaccacta gtgacttcac cttcagctgg acttcatacc actgatcaca gctctctggg 167340cctggctggg ctcacttatc taacccatcg tttgtcagcc tggccataag gatatcacag 167400gaaaaagtgt taaatgctgt ttctaaagtt aaggcaaaca acatttagta ttctcccctc 167460atcaaagaga ctagctgcct catggtagaa ggcaatcagg ctggctaagc atgacttccc 167520cttcatatat caatcctgac tactctcaat ctccttctta ctcttcatga gtttcacagt 167580agtgtccaac tctgtggtag cctgacctcg cagcaagcag aaatggcaga gagagtacag 167640aataataaag acaaggaatg ttacagtgct ggtgtatttt tagtactcaa ggcttggatg 167700cagaaaaaaa ccagcaacaa aacaacctat tttagaacta aaatgcaaat gtaccaatat 167760tttttaaggt ttttatttca ccatcagtaa ctggaacaag aaaatccctt ttaaaatatt 167820atttatactc cagtcactta caggtctatt gcaaccactg tgatacttat tctcctttcc 167880tctcttgtag gacaaggctg aatgggactt cccacggagt aaaaaactgt aactggactt 167940aatctagcag tgattttaaa ttgtgctgct tgttaaccag gcataaatgc ctagatgtac 168000caaactatag agtaaactga tcacaggtat aggagtacaa cagcagaata gaaagataca 168060gcctcacatc cacactgaga aaggtgagga tgaaagatga attcatgaga aagcccgaga 168120aaagtgactt gctttatcag aaaaatttta tgaccatttg tcagagagca acctatttgt 168180ggccagcagg ggttttgatg ctgcaacaca ttgtttctac cattctgttt gcagtaagcc 168240actctttcct cttctccacg tggcacagca aaaccaagag caccttttct ttcccatgtt 168300ttctcccctt tgaaactcta ctccctctta ctaaacagga tcttgcagca gataccatag 168360agtgtccaca cagtttgggc acccttttgt aattttcctg tatttttgcc caatctttgt 168420agtttgtagt acagtaatta ttgctattaa ctgctttctt acctctccta aatattcagc 168480ccaacaggta acagcagttt cagatatgta gcaggaacat ttcttaacca cataactaaa 168540cataatagca ttcgatagat ttctgaagaa agaaaatgag attgatattc caggtaggaa 168600aaatgtgcac acttagagag agagcatgtg aaaaaagata ggtatacatg tcactcctga 168660aaccttgtcc tgtgtatgta tcagtcacat tcaaagtgaa tagcaccata aaaatttagt 168720tctagttcta gatatggttt tgtactcctc atacaaatgc ttttgcacat aggttgactg 168780catgctgata actggtttac tttccagtgt cataactgtg agcagagggc actcctaagg 168840caattctgag taaccaaaca tgtactagaa aatatataca atgattgcaa aggaaactga 168900tcaatgcatt tcatccaagc cacgcagata aaaactggaa ttgctaatta cattggttaa 168960aataaaatgc ctgtgtacag ataactgtct tgcattatat ctttactgat ctgattccat 169020ataacatgct acttcgactc tctactacaa aacaacaagc agtttcagta aatcaaataa 169080ttacaagcag aacttccaag cagcttggca gcttttcgtg agtggcccaa actccctccc 169140ttcatagaag ataataagct tggcacagtg cagggcctgt aggttatcta aagcatcttc 169200acctcctctg ctaggaggaa atgcattctc ttaaagcacc acccaccagt tcgaataagt 169260accatgctca cgtagacttc atcacttgct ttaggtgact gtgggaatac agtctgagtc 169320tccttgagga tctgactgct gagacataaa aggattaagg gtaaaatatc acaacataag 169380ggctttgtgt gacctgacaa ccacaagtat tcaaatcttt ccactctgaa atttgacagc 169440aggatgaatg acactaacag cctacttctg tactcctttt cagctaaatc tttgagatta 169500gtttctctta attccctagt tcatagagta aaaagaaaag gaaactgtaa aattatttca 169560acgtgtccct tgtcaatgca ctgcaactct attcttttct gcaattccta catacttgca 169620gacaaagatt gaggagggca ccactgggaa aaaggcatac caggcttcat gtttcaggac 169680acacaccaac taacagtggt tttgaattgg cctctctacc ctcatctctc taattctgaa 169740agccagtgac ggagagaaga aatgggaata aatgagggaa caggcatatt aatacaagaa 169800gaggatgttg ggaagagaag ggaggtgcaa ccatttctac atttccaaaa gtattcgttg 169860cctcacagac cgccacagat cctgactgaa gaaacatctt aactcttccc tctctcacag 169920gatttcaggt agtgtaaata ttcttatcta aataaccagc tttattttga cagtcttaaa 169980aattcatata aattttccag aatggagagg gtaaatggta acttaaatgt catgcaggga 170040aatggagcac tcaagtcttc atacaaactg cagcctttag gaaaacactg cacaaagggc 170100acagtgttca ggatgcacat ccagactggg caaaacctgt gcagcacata gagagcacaa 170160gagtttcctc acttgcaggc agggtgccct aatcaccagg ctaccagctg atttaggatc 170220agaggactga acacagaacg cagatgataa tgcactccta ctgatcctgg agttgaacac 170280acgtgactgc atgactccat aacacagtgt tttagacaat cagcactgtc caagccactg 170340ctttattcgt gttaaataac tactggatca gcacccagcc ttttcctttc ctgaatgacc 170400aaagtggcta ttagacaggc agtgctgata caaacacaca tgctctctca agccctagaa 170460atgcttgatt gctgtgtgca aattcagttt tgacacgctc tggcaatagt ggagatacta 170520gtttgaaatt ctttaagcaa aaacattcag ctgagcccag ttctctagtt atgctctggg 170580agtatctggc tgatcagctc ctccaggcac agcagtaaga aataccaggc ttaaggtaaa 170640atgcttaagg tatgagctag gctgcagggc attcacctct gaagcacacc tgagaatgat 170700ccaaagctgt tttggagcac tgggaagctt tgatatccaa atttaagaat cccaaaagct 170760ccagctatct ctaagactga acagcagctg gttttgagga ctgctgcttt tgatttcagc 170820atgcaaattg tgagtgaagc tggactgaaa attccactca ttctcattga cctaaggcaa 170880gtctttcact tgatgcaatc ctgcaccgac aatggaaatt tctgcattta tcctaatatt 170940tgtcaacagt actaaattca tctaaaatat gttagttaga accacgaacc tattctggat 171000ccagctatga aaaattactt taaccctggg gtttctatgt ttttgttatt atctttgatt 171060ttgtcccata aagagggaag cagaaagaga atgggaaatc tgcaggttct gataaaccag 171120cacgaaagta gaacagagct gttgcttaaa atgggaactg tttggagttg tatgtaagac 171180acaagagctt acttgaaaaa cagtcattaa caaatccagc tacagttgac aaatgcaggc 171240ctacgctcac aaagatcaaa taacaatcta cagatctcca tcagccttct aaagcatgtg 171300gcaaatggca tgaattcgca atagttcttt cagcagaaaa taatgcagtt taaggaagta 171360ggaataaaag ctcagcgcag cacagtggga aatatttcta tttcgctccc tcagaatgga 171420aggataattc taagaaactt aagtaaatgc tttttaagaa caagtttggt ttgggaaaac 171480gttcctaaaa tagtgtttgt ctgatgatga ataagcgaaa cggctgaaac aagaatataa 171540gcatttaaca gtgatgggaa agggagaaat agcgtaggtc ttaaaaaggg acttgctatc 171600tgactgtacg tggcgttttg gccattacag agccatccca tcagcgtgtg tacaagggtg 171660caggcaatct cctaccagca acttcccatt ttgttagatg ttgtgaagat gcagtcatca 171720cctccctctg ctaatcattc gtctcaggct gtattgatgg agtgtctttc agcatcaacc 171780aacagcttca ccctggacct ttacaaaaag ctggatgtaa cttccaaagg acaaaacatt 171840ttctttgctc cttggagtat tgcaactgct ctcgctatgg tctatctggg tgcaaaaggt 171900gacacagcaa cccagatggc taaggtaagt tctgaactta gcagtgtatc acttaaagct 171960ggctcccacg tgacttgcat tattcacctg cctacgtgga acaaaaagga gcaacatgag 172020agaacagtga tccgccatca tgttgggtgc aagaaaaaac actgcatgca gtcctgcagg 172080acactgttgt tccaacttat ttccaagagc ccccttctct gctaccacct ctacctttag 172140cttaaaattg ttctggcaga gtgaagctat ggacattaga gactgtcttc tcattctcca 172200cacccagtta gtaagtcctc tgaacccaag cttggctgca cagcagcagc agcaacatct 172260ttgcttgtga catggttagt ccataaataa ggtaatacac ccatgaagaa gggaaggttt 172320agattggatg tcaggcggaa gtttttcaca gagaatggtg aggtgctgga acaggctgcc 172380cacagaggct gtggatgccc cttccctgga ggtgttcaag gccaggttgg atgtggccct 172440gggcagcccg gtctagtatt aaatgtggag gttggtggcc ctgcctatgg aaagggattg 172500gagcttcatg atccttgggg tcccttccaa cccaagccac tctacgattc tatgaataat 172560ggggatatac tagaaatgaa aatatgatat catttataac cacttttgca aaactttcgg 172620tgatgtactc ctagattata tgcatttaca taaatgcatt tatctgtgta atgtactgta 172680gagttgtaca ttggtgcctc aatagtaaga ctacaaacca tcctatgttg tttgttctgc 172740ctgataacaa tctgaaaata aattccacat tgctaagcat gaattgacca tttctccaaa 172800tcaatccatg tctgagcaat cacattgatc tgttattaag tagtaaatga ctaaaattaa 172860tataactatg atacggttat agaatctaaa tctagaccga ggtcttgttc tctataactt 172920taatagacta acatttgtac gatggctaaa ttatcctaag tagaaaacta acatcattac 172980gtaacactag agcacttcta tcttcacaaa acaaactgtc cttaagaaaa tttatcactg 173040ccacaggttc ttcattttaa ccagactgca agagaagaaa gtccttctga gatgacagca 173100ccttctctgc ggagcccaaa gagaagagac atggtattta ttttgacaag gctcacagaa 173160acaaatctcg gagaatggga cagcacaact gccttaaccc tctgaaattt gtctgtctat 173220acctactgtc ctattgcagt aagaactata cataaaaaat gtgaacaagc aggtaatatt 173280actgtaaaac tcacgaactc agaacttcaa agcagaacag agacaccaga agcattctga 173340ttgtctttta cactttgtta cttgctttta gctgctctat ggcaatagga atacctaata 173400ttttactaac ttcaggattt tttttttttt ctgggctgta aaacagccat tgccttcaca 173460tctgactcca gtcctgctct cttaaactct gttccacctt tgctatgcat gcacacatca 173520ggcaaacaaa attcccctgt aagctaacac acactgattc acccttgctc atcagctccc 173580cagagacttg acagcaggag gaactgcaca gaactcctta cgcctcaaag gtctgtcaac 173640tcaaacaagc tgcaattctt tagcacagaa actaagaaag ctcaaagaaa ccaagttaga 173700aaaactggaa ataaaaagga gcagttacat cattctccat ctagatggcc gtggattaat 173760atttaagata aatcattatg tccacattga ttttagctat tcactgcctt tctaagttgc 173820acagcaaggt ctgcctgatg tagccttcca gacatttctc tccattacct cttcactatg 173880tacccattgc cttgcaaaac ctatctacta tcctgttctc taccaagttc ttccccacat 173940gtcctttctg aaagcctgta cctctgcctg tgtgaaaaaa taccagagga aggaatgcct 174000cctcaccaat cttatgacaa gcccccatgc atcagcagca aagaatctcg gtgtctcaca 174060tgtagcgcat ggtacatgcc atggagcagg aaattatact gaagcagctt atccctacac 174120tacgaaagca acagctgaca agcaagctcc tgctccctaa aaccatcacc aaggacattc 174180tggacaggtt ccttccaaca tccaagcagt aacagcaaaa ttcacatcaa atagaaaatt 174240cgaatcaact caattacatg acatcagtat ctgtctgaac agagtagctc ctcaaaagct 174300gcaatgttgc cttaatgatt tttgtgataa tcaaaattag gcttgactgt gactggaatg 174360agatgaccca atatcctggg tgcaccatct gaggacagct gatgtcctca aaggtgtaag 174420cagctccgca gaatcaaatc ataaccccac agacataaaa caatgtatcc agttacacac 174480agtgctgcaa aagtaccaga tacccgaaga agagctccat ccctgcacac actttttaaa 174540ttaaaatgac ctggggattt taaataacca tagaaagtgt aatgcttcag ccaaaaatat 174600ttcaatacta attggtgcct ctttccaagg gtcttgagta tgaggaaact gaaaacatcc 174660actccggctt caaagaactc ctgtctgcca tcagcaaacc tagaaacact tacttgctga 174720aaagtgctaa ccaactgttt gaggacaaaa cctacccatt actgcctgtg agttgaacgt 174780ttctgcttaa gaatgttttg acaaagagta tttgacgtac atatttgaca atcataattt 174840tccaaatgtt ctgcctttta aatctactgc agcctttaaa actgtaagag ttctacagtt 174900gaactacaga aagctcctgt ctcttttgag cctatttctt gttagcatca ctgttggttc 174960acttctcctc taaaggaagc ttctccatat cagtggtcat cttcctcccc cttctcacta 175020tctattacat tctagcattt ctgagattgg aggccagagc tatatagaaa agtcacacca 175080tacctttaca gagcggcata ttaacgttct cccttttatt acactgtcat ttccttcaac 175140ttttcacatc atatttgctt gtttgactat tactgggcag attatttttt tttttcatgc 175200aactgtccat tagaacttca atacctcttt tctaaagcaa taatcatcct tattagtatg 175260ctttgctgga tcattttcag atgtttcaat attgatactg taatctattt ccttcttctc 175320ttagtttcta gccttttaaa caagatcttt ctctgtagtc ccacggatac tcagcttctt 175380caaaagcctt tggtgagaag tcttccaaaa tgccttttgg caatcctaat tggctgtagt 175440ggtttgacct tctcatttgc aagcttcttt acaccttcaa aaacctccaa aagattcatg 175500atgtaggact tattgttaca aaatgctgtt ccaacacatc atgcttacct acgtgcccag 175560taggcctgct tatccatttg ccctgtacag actcatgagc ttgtaattct gtgtatcttt 175620gaaatctttt taagaaatgg cactgtatta atcatcctcc agtttcttgg aaggtgctgt 175680taaatgaggt tagcgttctt tcatcttgga gttaagtttc agtctcctga gtaatttctc 175740agtcattaaa ttcaatcagc cttactgaat gcttgttgct catgttctgc ataattttgt 175800gtaaccgtgg tttaagacta atcagggaat ttcctgccgg gaatctccat gaacagtttc 175860atagaagaca gtaatgtaaa agtcagttct agtttattct accataacat tcccttcata 175920tctgaccctc tactgacctc acaaactctt ggcagcttga ctctgaagca aatcttaaaa 175980cattgtttca actgtccttg aagctattct ttcttcttgt actgaactct gtcataaaac 176040ataaaaatgt acttcaagtc agtctgagaa atcaaaaata atttaaaaaa atgtgtagaa 176100tgtttatctc ataggatttc aaaattacaa atttgcatgt tggatttaaa acacaaaact 176160ttcaagcatc attttttgtg aaacacaaat actgaatttt tgatcagtcc ttgcttatta 176220tttaacacag aatcataatc acagaatcat agaatggcct gggttgaaaa ggaccacaat 176280gatcatcgag tttcaacccc tctgctatat gcagggtcaa caaccagcag accaggctgc 176340ccagagccac atccagcctg gcctggaatg cctccaggga tgggcatcca caacctcctt 176400gggcaacctg ttccagtgtg tcaccaccct ctgtgtgaaa aacttcctcc taatatctaa 176460cctaaacctc ccctgtctca gtttaaaaac cattccccct tgtcctatca ctgtccaccc 176520tcataaacag tcattccccc tccagtttat acactccttt caaatattgg aaggccacaa 176580tgaggtctcc ctggagcttt ctcttctcca agctaaacaa gtccagtccc ctcaaccctt 176640cttcatagga gaggtgctcc agccctctga tcatcttagc agccatcatc acagaatgta 176700tcagaatttg ttttacgggt tatctagttt caaatgaatt acattttctt ccagtatgta 176760ttagtatgta ttgcatgatc tgttgagatg atctttttct actatttttg tgcttaaatt 176820taactatata agcatacatt ttccaattct atccttcaga aatttttaca actgatcaca 176880aggtactacc aagcaaagcc acaagctgta aactttaaga cagatgcgga acaagccaga 176940gcacagatca attcctgggt tgaaaacgaa actgagagta agtatcgctc tgatggcttt 177000ttcttttctc acttcaaaat catttgcatt tccacttgaa ttgctcttgc agtaagggat 177060ccataaagga tggaaactgt ggggaaatga tgaacaaatt gcagttaaat gtcttgaaga 177120aagccaacca ccaaaactaa ctgctgcccc ttgcaaagtt tttcccttga tttttcatgt 177180catagtctct tctgaagtat ttctgttcat aaggaagcag agtggatact acatggctcc 177240actctgatca gtgaaggttt tacttctgca agcttcaact ggttgcagcc aactccagag 177300aacttccacg ctttacacac ttcttaacat cttttactac taaaactgaa ataaatatgg 177360tttaaaaaac agtgatgctt caaaagccat ttatgtatgt acgctgtgaa aaatgcacag 177420ggaaaaaaaa tctctgagtg taaacacttt tgttagatag ctaggcatag agaaagcaca 177480tctgaaattg gtgagttgtg cattcgcagc gaattaacag tcctatctat ttgattttta 177540agggaagatc cagaatctgc tacctgcagg atctcttgat tctgacactg tattggtctt 177600agtaaatgct atttacttca aaggaaactg ggaaaagagg tttctggaaa aagacacatc 177660cgagatgccc ttcagattaa gcaaggtaaa ttccttcaaa atgtctatta tggcagagca 177720agaatcctct aaatatttca cctgcatttc acatcccagt acaacactac ttacagcagt 177780agcagatggt ataaactctg agaacagcaa cagtgaaaat aaatcagcag tctcatttat 177840acagatgcat gagattagga ttttcagtta agttagtagc ttcttggcac caaaacagtt 177900gaaaacacca tggttaagca gcttaaggac aagagaaagt ttctctaagt actgagatat 177960cattttcaga aggaattgag ctaattctga gagcagtact tcgacaccta ggtctctttt 178020catgcttttc agacagaggc tgtataatgt gagctcaagt agcctaagtg ttctttccta 178080atgccctggc cattgcgtaa aacctcacgt ggaattctca agagggtttg tcattttagc 178140cagatgcgta tggatgatgt gttcagcatg cattgtgggc acgactgagc ttacagtatc 178200tcagtgattg tgcatggaca atttacagta gctgacagca tgcatacttt cggcttgtgt 178260caaaggtgag caaaaagaat tttcattcag aacacgttgt ttgacatgag attacgagtg 178320caaacacctt ttgtatgtct ggtgatgtga agcaattgtg tcgatactgt ggctgtgtta 178380tctgaaacct actacattgc atgcgcagtt ttaggacctg taatagtaca cggtgcacag 178440aaagggtttc attcacagag tggctgatag caaagcctgc aaacagataa gcttttgcac 178500ttgtgtaaca atggaaaaga gagagtggat atatcagtga aggtctctga gcataataca 178560gcgtaagagt tcagatgatt actgtctaac gcgatttcag ttggtaatcc aaacctctac 178620agtttgggaa agagaaaaaa caagcagagg tcacagcaaa tatggtccat aggtaaattc 178680aatcaatcag tgctgtccgg aagcatacaa aagagttgat gacatccaga gaatgaaagt 178740cagcattttt ttcccctccc aatcaacaca ttcactcaag aaatgtaaag ttttggggaa 178800aacttgaaac atacaaagta gtttcttgtt taccaaagct aactctttca aaagagtgag 178860aaatacattg catgtaatta tgttatcagg tggtgtctgt gctttttttt tctttttttt 178920cttttcttct gaagatccct ttgactttga aacaggagaa atggcactgg gaaagaataa 178980tgccaagtct tatacttgtt tacaattttt ttttgccttc agttcaacaa agcaagtaat 179040ctttaccatt caccttaagg aataagtaca actaatcttt ttcttttctg ttctttttta 179100acatctgaat catttcacag accaagacta aagcagtaca gatgatgttt ctcagagata 179160catttttgat gctccatgaa caaacaatga aattcaaaat tattgagctg ccgtacgcgg 179220aaaatgaact cagcatgttc gtactcctac cagatgacat cagtgataac actactggtc 179280tggagctggt aaaactgaca tactgcatca cacgcactac aaagcactaa cagaaataga 179340tgaaaacagt gaggaagaat gaacttcaaa tgacacaatg actgctcagc ctaggtttca 179400gggcatctat taatgatgca aaaatacaaa tctacctgag gatacaccta aaaaagtatg 179460cccactctac tctcttagcc tattcggtgc ctccttttct acctccagaa tagcagaata 179520acgaaagcaa gaatcaaatc taaaccactg tgccccagaa ttaatcttct gagggcaaca 179580ctaaccagtt ttatgtcatc cgcagtccag atttccacct gatactttgt aacgaggctt 179640ttcaaactcg gggctgactt accttgaccc atgaggtatc agcagccact catgaccgtg 179700ccaggattag ttcctgaatc taaatacatc agagcttcag aatctaaata catcagggca 179760aatatctttt tatttgctct tgaggtccca tgcattccac ttacttacca ctactaagag 179820aaatgcctta caaattcaca cataccaagc acttattaat gtggttaagt tggacactgc 179880ataaaagcaa cacttctcat atccacctcc aaaataatga attattctga aggttcactc 179940tacacctcac tgcatttaag gaaacagata gaagtacagg tcactcagca ctatgcagga 180000tcacatccta agaatatgca gcacatttca gctgtactca cagctggtag ttggaccttt 180060taaatctaga gcattagaca ccaatgtatg catgccttct tttttctgtt gcattatgac 180120tatattctta taaaattcat tgcaggtaga aagagagctg acccacgaaa aattagctga 180180atggtccaac tcagcccgta tgatgaaagt cgaagtggaa ctgtacctgc ccaagttgaa 180240gattgaagaa aattatgatc ttacatccac tttgagcaac atggggatac aaaatgcttt 180300tgaccctgtt caggctgatt tcacaaggat gtcagcaaag aaggacttct tcctatcaaa 180360agttattcac aaagcttttg tggaggtcaa tgaagaaggt accgaggcag cagctgccac 180420aggtgtcctg gtgttgaggt caagaacacc tagagtaact ttcaaagccg accacccttt 180480tctcttcttc atcagacaca acaaatccaa aaccatcctc ttctttggca gactatgctc 180540accttagtca gagtcactcc ctgctctaca gagcaggaga tgctggcttg ccagctcaag 180600ggcagagctt gatactcctg ctgcagctga gggactaaga cctgcactct ttcagactac 180660acattccaca gcccaaggca aagcttcaac tactccagat

agccatagca gtgcctgtag 180720atgcatttga ttccttcctc ttgcagcagt agatacaaac acatggcact atcttcgttc 180780tcacaagtag agcacctgat tcaggtgtgc atcttcaccc ttccaccctg ccataattag 180840cccctgctcc tctgtagctc ttgactagtt ctttttgtta cagaggcaca cacagcccaa 180900gcttaagtct ttaccagttc acttccattc tactgattgc ctgaaagaca taacaagcac 180960acactcccac gtgggctatt tcctcgcacg gagttacagg tgtgacagaa gagcctgacc 181020catgctgctg actttataca aagcagcacc tgcttcaaaa atagcagtac tgataataaa 181080caacccctcg tagcttgatg gtgctttctg tcagctctac caggagggga aggcagaagg 181140ggaaatcaag cagcgacaag aggctcgcgg aggtagcgac ctccgagcta aaatggccgc 181200ctcccactgc tgcagcgagt gctcagggcc gctttccgca gctgagctcc agccctctcc 181260cccacgatgg gcggcccgtg gctaggcaaa aacttccggg aggagggcgg ggcagaggcc 181320aggggaaagc tggtgctcgg ctgggtgagt gtggagggtc tgtgttgttg ttttctgcgg 181380gaaacacgca ttggtttttt gaggggagac ggtagcgttt ccctcgcggc ggcgctctga 181440gcggtttcgg cgggcgcggc cgccgggcgt tgaccgggtg ctggaggcgg gaggggcccc 181500gcagagttcc gcaccgctgg aatccatccc tgtcatccag ccctgcctct gtgggttttg 181560tggcaaacag gcggaaatcg atggagaggt gcgagcttca gcctgttctg agtcacaggg 181620agagagcttg gccaattgtc ctgcgcccag ccttattgga gctgtaaggt gcacgggatt 181680aaatcgctcc tgcttcaggc agaatggaag gactgtttca gtccaagttt tcttttcatc 181740agtgttttta tggctatggg cagaaggaaa catgagtaca gctgcagctg ttgaacgtag 181800ccaagctcct accaagaatt tgtcttagag gaaacatgcc tgaggaaact tgctgctacc 181860gcttgtttga gatgatgaat cattaataca aagtaggcgt tggctctgta ttttctagca 181920acgtaccaac accaggcact gccttagggg aaaaaaaaca aaccaccttt actactagtt 181980gatatcctgc gatgtctgct ggcacttatc tgtaacttac tccacgttct ggcactcgtt 182040gctccttcct gtaggtatgt agtataactt cggattagtt agctacctgc tcggctgacg 182100tatgtgaagt ctgacaagca ctgagctacg tatgtgccat gaagttccca ataaaccgtt 182160tactttattg cgtctgtttc catcgtgtag acaataaaag gcaaactgca gtggactttg 182220attttgtacc acagcaggaa accccagtaa tctgtaatgc tgaccagata aatttcgttt 182280gaatattgta gatcgagtca ttcagttgga ttctggcaga ctgactgcta ggtctagaac 182340acaagtgaag taatcttgaa gggaatactg aagacacaca gactttgaga aggtgagttt 182400ataattctgc cattctgata cctttctgct ttggttttcc tgtaaagcaa ataactgtct 182460ctgtggagcc aaaggagact tattctacca agtcctagta tgctcatctc aaaaaatata 182520gtattattta ctccatgaag aagaccaatg acttttcctc actacaagaa agacattgag 182580gtcttggagt gtttccagag aagggcaaga aagccgtgaa gggtctggag cacaagtctt 182640actattgagt ggctgagggg gctgggattg tacagcctga agaagaggag gctcagagga 182700gactttatca ctctgtacag tgacctgaaa ggaggttgta gttggcctct tttcctgggt 182760aacagcaata ggatgagagg ggatggtctc aagttgtgtc aaaggaggtt cagatgggat 182820attatgaaca atttattttc cgagtggtga ggcactggca cagactgccc aggaaggtgg 182880tggagtcacg tctctggagg catttaagaa acatgtaaat atggcactga gggatgtggg 182940ttagtggaca tggtgggaat gggttgacag ttgtactaga tgatcttata tttgctttat 183000ggtttatatt gagaaatgta aaagacagaa ataggttgtc agtttgtgat caaataaatt 183060taagccaatc ttcatttttt tttttctcct aggctttgaa ccatggatag cctcagtgca 183120gcaaattcca cttttgctct tgacctttta aatgagctgc gtgagaaaag cagcacaaag 183180aatctattct tttctccttt tagtatttct tctgctttgt ctatgatttt actgggttca 183240aaaggggaca ctgaagccca gatagcaaag gtatgtatcc aaacgtaatg tattggattt 183300gatgcatata tcatctactt aatgatatat gaactacaga tctgagatct gtattacagt 183360ctgtgacctc taattgctga attgttacag tcattctggc ctcagaggtc agaagtcttc 183420cttaggtatg tacataagca gaacctattt ctattgagtt tatgtatagg acttactgca 183480gtgtgaaatt aagagattcc tgttttttgg ggtgtgtgtg ggtttttgtt tgtgatacgg 183540agatcttcct tttatatgtc attaacaggc acctggaatt tctttttttt tttacttaca 183600tatttgtata tttagagcta tagatgaatc tccagttaca taaaataatt tactctgtaa 183660tctttttggg cttaatatca gactttgcat acttcaaaaa tgtagccaga taatcaaggg 183720aaaaaaaatc caacatacaa gcatgtcatg ttaaacagtc ccagatttta ggaaacaaac 183780aaaaaaatga tcagttgctt gttcagtgta atagcttttg ttttcacaac ctgtaatctc 183840aatcctggaa catccagaag aaagaagtga tacagggcta agaacatagc tctgaagttc 183900cagagaatac cccagcaaag attcaatggg gcaaagctgc gtggccagtg aagagtaaaa 183960ttcataatgt aaacttgcaa ttaaattacc aggagagcag ttaaggagtg cagtggtggg 184020cctgttgtgt gacagtaggg tcaaatctat cattaactgc agtgcagttt attctacgtt 184080cactaaggtg cgtgcctgcc tctctctttc tggtattgta atttggagta gatcatcaat 184140actttttcat ttgtagctat ggtagtagtg atgaggctga atgaggatga agctgatgtg 184200ttgttttaat gggaatttaa atatttgctt gtgttgacat cggctccagc agcctatttc 184260ctgttatcgc ttgaaggatc gggtttgcat ctaaggtatt aaataagatg ctttggtgct 184320attataatca gtgtgaaaaa ttatggaaag ttgttttttt ttatttaatc ttcaggctcc 184380tttgtttctg gattttaaca gttttgctag gttttatagg gtggagatta taaatcctca 184440gttctctaag aagtactgtg tacagcatta agaaaagggc agaatgtgtc tgcactcaga 184500cttctttgga ggctggatgg gttccttaga aagcagggag ataaaccagg taacctccat 184560agcttccttc caacctcaac cattgtgtga tcctctaatg cttggacaaa atgaagataa 184620ataccactca cttttcagca acgtaatttc ttgcttatac aacatctgtg tggatacatt 184680gtacgtgact tgtgtaatga aaaatctgct ggcttcaagt ctcaaaactc atttaaaaac 184740agaacaattg tgctgatgca agtgtgtcag agattacgtg gactccacag aaggtatttg 184800tctctctgca ggtgctttct ttgaacaaag ctgaggatgc tcacaatggg tatcagtcgc 184860ttctctctga aattaacaac cctgacacca aatacatcct cagaactgct aaccgacttt 184920atggagaaaa gacatttgag tttctctcag taagtaaaca ttaaatttgg gtgttgtgaa 184980gtataatgta cttgctagct attccccttg aaggttagat aaaggctttg ggttttactc 185040tccaaatttt tctaggctga gacttacaac ctgagagtct atgcaaaaag caggatgtga 185100acagaatgga gaagctactt ttagattata tgaatgcaca actggtgcaa gaccatgaaa 185160aaaaactaaa tcttctaggt ttcttggtcc acttttggtg ggttctagga tcaaatgaat 185220gacaaatctc cttgcctttg ataacctgta gctatgatga aaacaactgt tactgctgtc 185280cagcatgggc agaacttttc ttttttctta attaaacaat ccagagaaca tgctgagagg 185340agtatgtgac tcttaatatt ttccttataa gtatatatac acaagagggc acaggtacgt 185400tgcatataca ttacatatac attataacat tgtatgttct ctcactcaag caaaaagaac 185460aaacggaaga aacaaaaaga aacaacccag acaatcattt ctcagttgag tactgtagaa 185520tgttctggtg tattaaagaa gacatttgac ttcttaataa caaagaggaa gataattcct 185580agctcagatg gctaataaaa caactgataa gaacatgtca gacaaaacct gaatggcttt 185640atatcaagct gggggaagag aggatataga tttttctcag tgtacttaaa aacatctgtg 185700gctgaatgtc agtaaaatgc attgctaaaa agctgtttta aatgttcatg gcagtcattt 185760atagaatcga gtcagaaatt ctaccatgct gggctagaac agactgactt caaaaatgct 185820tcagaggatt ccagaaagca aataaatggc tgggtggaag agaagactga aggtgagtgt 185880tctgcagaac tccctgctgt atgtaatgtc agccaggact tgcataaaca gctctgtcaa 185940ggtgtaatac tgtcattttt aaagcaaaca caaacctcag ccattgtgct ctgtctctgg 186000ttggggcata attcccatat ctgatctatc gttaatacat attagatact ctgtattgca 186060acagttgctt acgtaccact gttcaatttg tgttttctaa aggtaaaatt caaaaattgt 186120tggcagaggg aattattaac tcaatgacca aacttgtgtt ggtgaatgcc atctacttca 186180aaggcaactg ggaagagaag tttgacaaag agcgcacaaa agaaatgcca tttaaaatta 186240acaaggtacg ctacgttaat atgctgacaa tacaaaggtc tttgtaatac agaagacaaa 186300aattgttcaa gcagatttac ctaaggtagt ctgcatggag ctccctatgc cctgtcccct 186360tagtatgaac actctctttg tttagtttct gttaagtttc acataattac taaaaacttt 186420aatatcacat atttatttta tactctctct tttttttcct ttactctttt tgtttgtgtt 186480tcagttggtg aacttgacta tgtcagtgta aaatctgcat gggcaaaaaa cattcatagg 186540ttccaggcag aaaagaactt ccgtgtgtgc agaaatgtct gaatatagca gtcatcttca 186600gtcagaatgc ttttctttct gctgtgtttc taccactaaa ttgatagaaa tgaaatgagg 186660tgaagaaaaa aaaaaccact ctcctttgaa ggcctccatg cttgactttc ttttgcttct 186720aaaagtgcag cagggcaatc gaggaggact ttatgtacta tcattaatag gctacggctg 186780ccccttagag gtcaatttca aactctggat gtccacccag gtgtcgtgag agtgaactgc 186840taatgtgaat tgcttaagaa ctcacctgct taaaataacc acaatgcaaa attgaagctc 186900tagtgcctaa tttcaaactt cagtgttgaa atatatacag gaatgcttga aactgctaat 186960accacttttc aaacagggaa taataatatt gctcttgcca tactgtatgc tatagcactt 187020agaaaccact gcactgactt ggttcctgtt aggaagggag gttttttatc agtttcccac 187080agagatgtca cacaaaaccc aagcttacat tctgcttaga gttttttcct ctccctcctc 187140aggaggcaaa tccagtgctg tttctctggg tacgaggctc agcctagttc tgagattacc 187200ctttcctttg cagacacaca tttatttttg aagactgcag tttttgggat gcagatggct 187260attggaacaa gttgtaagat gtgagactgg ggaatgctgc cttggctcat caagtaacac 187320gctgttagat gtgcaaccac aaacctcttc ccttacaaaa ctaagtggct taaatttcta 187380ttttcatcct attgatgact agtcactgat gagctacaga agtcaatgag taggctcaaa 187440taagcaatga aaaatccaaa gggcaaagct gaagttttaa gctagttatt ttacagtctg 187500tccaggagta gttacttaaa cataccagta gtcttctgag cattctgtga catctatttt 187560atctgtgact tttgcacttt gttgtgactc actggatgat tctagcatgc agtgtgggct 187620tttctttgct tatccatcat tttcatgtgt cactgattgc tgttgcaaaa tcatttccga 187680catattctgt tctcagagtt catggcagtc atttatagaa ttgagtcaga aattcactgt 187740ctcaatggtc tttcctttaa aaagaaaaaa cggtgaaggt aaggggaaga agggatttag 187800actccacaga aaaggaggaa aataatgtag acaaaagtaa ctgatgctcc atgcaaaaat 187860ggagagagat gggggagaaa ctggtagtaa gaagacaaaa gattaacctc tccatgtgcc 187920ttttaacaat tcaaagtgat gctaatactt tcctagcatt tttagtggct tagtaaaata 187980tttttgttgc cctacgtcag agtaattaga gacacatgga gtgaaatgaa aatatcgaag 188040ttgaagttat tttgtattta tttaaagcaa ggaatacagg ctctgcttat taccaacttt 188100gtttagagct tgtcactact tctaaagtga gcaaatatgt attcttgctc ctttacccta 188160aagcaaattt cacagatatc tccaattaac aattaaatct cagggatcct tacttctcat 188220ctcttgcttt acgaaagagt gactgtgcta tactatgtta tgcagtgtac ttagttctct 188280gtgcagtcaa atagtaaaaa gccctaagta actagatgcc tgcttcatgt attaggactg 188340tcatgccagc ccagtagtaa ctcttagtgt ctctttcatt ttagaatgaa accaaacctg 188400tgcagatgat gttcagaaaa ggtaaataca acatgaccta tattggagac ttggagacca 188460aaatccttga gatcccttac attggtaatg aactcagtat gatcgttcta ctccctgatg 188520caatccagga tgaatctact ggcttggaaa aggtaagtta ttgagctcag tgcaaagaca 188580gtttgtgtcc tgccttggaa gagagtttgg tgctgcacat ggattcacag ttcagtttca 188640gagctattat atcattgatg ctcaagactg actgaaatgc tccttgtgtt tctgccccta 188700aagtggcatg ccatctatta ctactggcca agctatgtgc tgctgtgcta agaggctctg 188760aaagaggcct catcagaagc tgtagttatg gtgaagccat agtatgatga gcaccaaatg 188820agagggaatt tggggcagct cttaggaagt ccttaccaga atttctacag tttgtcccat 188880aggtcatctt agtgaagacc tggcagattg tcactgcccc tctacttgga aacacgctca 188940cagaatagtc caggttccct tccgttgtga tgatagaata caagtcatgc tctggcctct 189000tgtttttttt tctaatgctg attttaattt aaaaagtgtt gtaagcaggt tttgtcacca 189060gcccgtgagc tgaaagatcc tgaaaggctg aagaactggg ttcagtttgt ttggggcctt 189120gtcagcagtt ctcccgtgcc tttactccct atatataaaa taaggttttt acaatctgat 189180aatgttttat aaactgaact ttactgtatc taccacgaaa aagaaaacac caaacaagaa 189240ttgacctcag ctgaagctgt agtctctagt aagtagaaac ctgtagtgac ttgtgctttt 189300gacttgggat cctgtaagct cctgaaaaag atgcatattg catgtatgtg tttacataac 189360acacatacac agacaaaagt agagattagt gcaaaactgt cactattctt attttaatta 189420cctaatgttg ggttatgttt cgttgctttt tttgttttaa gctggaaaga gaacttacat 189480acgagaagct gatggattgg atcaatcctg aaatgatgga cagtacagaa gtgaggctgt 189540ctttacccag atttaaactg gaagaaaatt atgatctgaa acccatcctg agcaacatgg 189600gaatgcgtga tgcgtttgac ttacggatgg cgaacttctc aggaatctcc tctggtaacg 189660agcttgtgct ctctgaagtg gttcacaagt ccttcgtgga ggtcaacgaa gaaggcactg 189720aagcagcagc tgccacagca ggagtgatgg tgctccgttg tgctatgatc gttcccgact 189780tcactgccga tcatcccttc ctcttcttca tccggcacaa caaaacttcc agtattttgt 189840tctgtggcag atattgctct ccctaagaag agagacagaa gagctaccat taacgcagta 189900atgtgatttc ttttaggata gaactgctct tttgcactaa ctgcttattt ccactgtgcc 189960tgaatcccct tatctggttg tcattttggg cttgcgtaga gtaacaaagc cacttacaca 190020tacacagcag ctaccacttg aaacagctgc cttacacttt gcacctaagt ggagttgttt 190080tcttgctggc ccaagaaaga tgaacatccc acttgctcag tgaacttcca cctgtcttat 190140attttctatt gcactttgct tttgtgtggc caccaggtag caaggtgaca aagagaaaag 190200aagtggattt tgtttctgac tatagtggaa gatatcttat gctctgctcc ccatttttct 190260tcctctcccc acttattttt aactttttct ttaatgtttt gataatagag ggagatgaaa 190320ggaggctttg gcgacctatt tgtaagagtt actaagcatc tgcactagac agaggtttta 190380ttataactgg atagcactta cacaaggatg ggaataaaag tatgtctgta acaaatgacc 190440ttagaggttt tcatggagta cggattctta tcttaacacc acatgtgcca cctgggaata 190500ttagctatca ctcacctact tcattagtct tttaaaaaaa gaatgttttt aaaaacaaac 190560aaacaaaaaa aaacccatag atgcctatgt agtatttaag tgacagagct ttatttttgt 190620ttttcagtct ttatatgttt ttttcctatt ctgggtttgt aaagcatctt tgttaatctg 190680aatgccaaag gtttcttaac gcagtgattt acgtgttttg ctgttcttga aagaataaac 190740aaatttgttg tgagtgctgt gggcattgcc cataaatttt gtggggtttt tttttttcat 190800ggctactgta aagaaaacaa gcaatcaact ttcgtgtagc ttatgcagaa ttcattgctt 190860aacagaggct tttctgaatg ctgcaagacc aagatgctta cctggattac gatggagttt 190920aggtttttac cttcgaagga ttcatagcaa ggagtctttg aggcaaaggc tcaagggatt 190980ttaaagacta tctggttcca actccctgct gtgggcatgg ctgcccaggg ctccctcctc 191040cctggccttc gacacctcca aggatggggc acccacagct tccctgggca gctgtgccag 191100tgcctcacca acctccaagt gaaaaagatc cccctgacat ctaattgaaa gttcctttca 191160tttattttaa agccattccc tcttattcca tcactatcag accaaggctc cctttatgta 191220ttggaaggcc acaacaaggt ccccttggag tcttctcgag gctgaacaag ctaagtgctt 191280gtttccagat ggggtactgc tgctgtgggt gcaactcctt ggccccaggc caaggagtgt 191340gccatgcctc agatgcagcg attagtcacc atttggggtg aggaagctgc caaagtgctg 191400cctgtcagac cgatgctcag tcagggctga gagcagcagt gggtagaagg gaagtgggca 191460gcctctgctc ccagtgcatt gtctgggaag ggggtggtag caagatgaaa agtagaaatt 191520tttctgaccc ttcctacgtg tccaggctgc tgctggagtg tattcatggt gctatgctta 191580aagtgaaagc aaaagcgtgc ttgtctaatt tgcttctttt ctaaattgaa aaggaaagta 191640atcacattaa cgtctaccat aaagcagaga gaagctgcca gaaagcttga gagaagctag 191700aagcagccat atctacaaat cccagtgcaa acaagaagga gggatcccag ctgcacaagc 191760aggaaggcag gaaggtttta cagcactgtc tgccgccagc ctttgcgtaa ccatctgccc 191820gccccagcat tgcacctttc aacccactcc cagagacctc acagctccca gtggtcctag 191880ctccagctta ctgctggctg ctctcctcct ggtttgatcc tccctagcag ctgccaagca 191940tcacaggagg taagtgtgtg cttgctgtgc ctctgcattt tgcagcctga aatgaatcca 192000gcccttggaa ctcgcactag ggcatcgagg agtgctttct gaagccttca ctgaaacttt 192060tatttttcag ctgcagccat ggagagcctg agcaatgcca acagcaggtt tgcacttgat 192120ctcttccgaa aggttaatga gacgaaccca tcaggaaaca ttttcttctc ccctctcagt 192180atttctactg ctctggccat ggtcctcctc gggtccagag gtaatacaga gacacaggtg 192240ctgaaggtca gcagcatttt cgcttgtttt attaaaatta aatgttgttc agttttagag 192300acaaggcaag gggaggaggg cgttatttgc gtgagcttgg ggcaaggttc ctgtcactcc 192360tgctgactct tcccccctgc tgccacctgc ctgctgcact ccagagccct cctcttgtgc 192420tcactgatag cccttctttc tcacttcatt tgggttaatt gatgaatctg gaaactaatt 192480tcactgattt atcagtctta atttaaaatc gattagcatc tccagcagca agtctttact 192540agagcttgtg ataggacatg ggggaacagc attaaacaaa aagggaagat ttaggcaaga 192600tatttgttag aggaagtttt tccactgaga aagtggtgag gtggtggcac agctgcccag 192660ggaagctgag ggtgccccat ccctggaggt gttcaagacc aggttggatg gggccctggg 192720cagcctgagc tggtgggtgg ctgccctgcc cacagcaagg cagtggaact gggtgggctt 192780taagttgaat tccagcccaa ccccattcta tgattctatg agccttttcc acagagaact 192840attgttttgc aatgtataca tacataatgg tatacatagt aatgctaagt gtatcttata 192900aataaaaaat aaaatataga gctgtattat tctaaggctg acaactgtta caatacatgg 192960tgatgttacc caagacccag tgttatagca gccaagcacc cagtatttct gaggagcaga 193020actcacgtgt ccattctcat ggtatcctta ggttgagcag cagaggttaa atgaaaatgg 193080tgtggctcct ttactggggg ctttgttgtg gacccagctc atcaatcctt tcccactctc 193140cacaacagca gttgcaaact gcaaattctc atgtaggtag cagtgccaat tccctctcag 193200actcatgttc aaaagggacc ctgcctcttt tttaatttgc aaggcaaaca cctgctagtg 193260caaggggaag tatgaaagaa ttgtcgctgt agttcctatt aacttatttg ccctatgatt 193320aagttcacct ttgtattccg aactttagga agaacttgtt tagaccatta actgctgcca 193380ttctttgtga aaagactata aaactgaatc actgcttgta gaaacagact ttgaacatac 193440attccttata actcaactgt cagccccacc caggaagaat ctactgagag cagaaataat 193500gcaagagaag catagggaag ttggagatag aaggttggga tgaatggttg gactgggtga 193560tcctgtgtgt cttttccaac ttcagtgatt ctatgattct aaggtgtttc agcacagtaa 193620ccttctgtaa tgcacattcc catggtataa tgtttaattg atgagaacat cagttaatta 193680aggagatgat gactgatgag tgtgaagggt gtttataagc atgcagaaat ccatttctgg 193740gatcataatc ctaccttaag ttggaatcat agagtacacc acggtggagg ggatccatga 193800ggatccagct ccacacagca ccacccacta tggtagatcc tgctgcccaa cctgcacacc 193860ttggctagtc agcttccctt caggtatctg tatgcacgct ttcatattat aacagctttt 193920aattttaagg tgatagttgt ctgtagaagc acttatattt tcataaaacc aaaggttata 193980gctctcacat tttcctaaca cctcaccttc cctgagtgct cagacaagct cagtagtcca 194040cggaggaaaa acatgcagac agcaccctat taggactctg gatcacaatt aacagcttca 194100gctgtggcta actgtattca gctactgctt tacaagtgac atggctggca cagcactaag 194160ggacagtttc acttgttctt tgatggttac agctttcagc ttctttctgc ttttgttttt 194220caacttaact accaaacaaa taccatacag atatgctgca tgttctctat aaatacagca 194280ttagcagtag ttagctcatc tctttcattt cagacgtttc attttgatga agttgaaaat 194340atacactcaa gattccgggc tctgactgca gacatcaaca gaagggattc ttcctgtctc 194400ctacggattg ccaaccggct ttatggagag aagtcctaca gctttctgcc ggtatgggta 194460cacagaccat agctgtgtgg tggaacctgg ggggaggctt tgtaacttca tcatctgttg 194520ctctcctgcc tccagaacgc gccccatagc aaaaatatca caccagcaag tccagatgtc 194580aaaactatct ttctgcatca ataagcagca tagctcaggt gttgctgtct ttataggaat 194640gcagccattt gagtatttga ggtaaaaaca tgactagaca tctaaaagtt accaggcagt 194700cagtacgagt gttgtacaca tgcctataga tgcagaaatg catatgcatc tggacatcct 194760aaaggatacg cctagaggat attacataac aaatcccttt ctttgatagt tcagttctgc 194820tgctttgggg ctcaagagaa attgcaagcc atgtaggttc ttagcttaga gtacagatta 194880gcaatgcccc attcctctgt ctgttgtttt ttaggctttt cattgctcta gtactatatt 194940acttaaaaca tttttgaaaa catttctctg gggggagatt gccatcatgt ctcaacagca 195000tgcctcttta caagggaact gtacctctgc atctatttag gtactgctat ttttatccct 195060ctccagctct ttctgggagt ttttgttttc ttagtcaagc tt 1951022285DNASV40 2aaagtctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag 60tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 120gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 180attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 240ctctacaaat gtggtaaaat cgataaggat ccgtcgagcg gccgc 2853498DNAArtificialHuman interferon alpha2b codon optimized for avian expression 3tgcgatctgc ctcagaccca cagcctgggc agcaggagga ccctgatgct gctggctcag 60atgaggagaa tcagcctgtt tagctgcctg aaggataggc acgattttgg ctttcctcaa 120gaggagtttg gcaaccagtt tcagaaggct gagaccatcc ctgtgctgca cgagatgatc 180cagcagatct ttaacctgtt tagcaccaag gatagcagcg ctgcttggga tgagaccctg 240ctggataagt tttacaccga

gctgtaccag cagctgaacg atctggaggc ttgcgtgatc 300cagggcgtgg gcgtgaccga gacccctctg atgaaggagg atagcatcct ggctgtgagg 360aagtactttc agaggatcac cctgtacctg aaggagaaga agtacagccc ctgcgcttgg 420gaagtcgtga gggctgagat catgaggagc tttagcctga gcaccaacct gcaagagagc 480ttgaggtcta aggagtaa 498

Claims

1. A method comprising: delivering to an avian cell a nucleic acid molecule comprising a nucleotide sequence about 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1 operably linked to a heterologous coding sequence.

2. The method of claim 1 wherein the cell is an oviduct cell.

3. The method of claim 1 wherein the cell is a tubular gland cell.

4. The method of claim 1 wherein the nucleic acid molecule comprises a vector.

5. The method of claim 1 wherein the cell is a cell in culture.

6. The method of claim 1 wherein the cell is an in vivo cell of an avian.

7. The method of claim 1 wherein the nucleic acid molecule comprises an internal ribosome entry site (IRES).

8. The method of claim 1 wherein the nucleic acid integrates into the genome of the avian cell.

9. The method of claim 1 wherein the avian cell is selected from the group consisting of a chicken cell and a quail cell.

10. The method of claim 1 wherein the avian cell secretes a protein encoded by the heterologous coding sequence.

11. The method of claim 10 wherein the protein is isolated.

12. The method of claim 1 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.

13. The method of claim 1 wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.

14. A method comprising delivering to an avian a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1 wherein the nucleic acid comprises a heterologous coding sequence operably linked to the nucleotide sequence and the heterologous coding sequence is expressed in a cell of the avian.

15. The method of claim 14 wherein the cell secretes a protein encoded by the heterologous coding sequence which is expressed in the cell.

16. The method of claim 15 wherein the protein is isolated.

17. The method of claim 14 wherein the cell is the cell of an avian selected from the group consisting of a chicken and a quail.

18. The method of claim 14 wherein the cell is an oviduct cell.

19. The method of claim 14 wherein the cell is a tubular gland cell.

20. The method of claim 19 wherein the tubular cell secretes a protein encoded by the heterologous coding sequence.

21. The method of claim 20 wherein the protein is secreted in egg white.

22. The method of claim 21 wherein the protein is isolated.

23. The method of claim 22 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.

24. The method of claim 22 wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.

25. A method comprising introducing into an avian embryo cell a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.

26. The method of claim 25 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.