U.S. patent application number 12/358659 was filed with the patent office on 2009-08-13 for chimeric hiv fusion proteins as vaccines. This patent application is currently assigned to HealthBanks Biotech Co., Ltd.. Invention is credited to Hsiu-Kang Chang, Chao-Wei LIAO.
Application Number | 20090203884 12/358659 |
Document ID | / |
Family ID | 40939452 |
Filed Date | 2009-08-13 |
United States Patent Application | 20090203884 |
Kind Code | A1 |
LIAO; Chao-Wei ; et al. | August 13, 2009 |
A chimeric fusion protein useful as an immunogen for inducing HIV antigen-specific immune responses contains a first polypeptidyl region and a second polypeptidyl region. The first polypeptidyl region includes a Pseudomonas Exotoxin A (PE) binding domain and a PE translocation domain. The second polypeptidyl region includes (i) a first peptidyl segment containing a fragment of gp120 C1 domain, located at the N-terminus of the second peptidyl region; (ii) a second peptidyl segment containing a fragment of gp120 C5 domain, located at the C-terminus of the first peptidyl segment; and (iii) a third peptidyl segment containing a fragment of gp41, located at the C-terminus of the second peptidyl segment. The second polypeptidyl region contains an antigenic determinant specific to one subtype of HIV. An intermediate polypeptide containing a non-Env, HIV antigenic determinant selected from Gag24, Nef, Tat and Rev may be included.
Inventors: | LIAO; Chao-Wei; (Shin-Chu City, TW) ; Chang; Hsiu-Kang; (Taipei City, TW) |
Correspondence Address: |
IPC Intellectual Property Connections, INC. 299 Old County Road, Suite 28 San Carlos CA 94070 US |
Assignee: | HealthBanks Biotech Co.,
Ltd. Taipei NJ HealthBanks USA Inc. Princeton |
Family ID: | 40939452 |
Appl. No.: | 12/358659 |
Filed: | January 23, 2009 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
61025094 | Jan 31, 2008 | |||
Current U.S. Class: | 530/350 |
Current CPC Class: | C12N 2740/16322 20130101; C07K 2319/10 20130101; C12N 2740/16134 20130101; A61P 37/04 20180101; A61K 2039/54 20130101; A61K 39/12 20130101; A61P 31/18 20180101; C12N 2740/16222 20130101; A61K 2039/5254 20130101; C07K 14/21 20130101; A61K 2039/57 20130101; C12N 2740/16334 20130101; C07K 14/005 20130101; A61K 2039/55566 20130101; C07K 2319/00 20130101; C12N 2740/16122 20130101; A61K 2039/545 20130101; A61K 39/21 20130101; C12N 2740/16234 20130101; C07K 2319/40 20130101 |
Class at Publication: | 530/350 |
International Class: | C07K 2/00 20060101 C07K002/00 |
Sequence CWU 1
1
252116PRTHuman immunodeficiency virus 1Val Glu Lys Leu Trp Val Thr
Val Tyr Tyr Gly Val Pro Val Trp Lys1 5 10 15225PRTHuman
immunodeficiency virus 2Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala
Pro Thr Lys Cys Lys1 5 10 15Arg Arg Val Val Gln Arg Glu Lys Arg20
25335PRTHuman immunodeficiency virus 30Cys Thr Arg Pro Ser Asn Asn
Thr Arg Lys Gly Ile His Met Gly Pro1 5 10 15Gly Gly Ala Phe Tyr Thr
Thr Gly Gln Ile Ile Arg Asn Ile Arg Gln20 25 30Ala His
Cys35435PRTHuman immunodeficiency virus 4Cys Thr Arg Pro Asn Asn
Asn Thr Arg Arg Ser Ile His Ile Glu Pro1 5 10 15Glu Gly Ala Phe Tyr
Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln20 25 30Ala His
Cys35554PRTHuman immunodeficiency virus 5Gln Ala Arg Val Ile Ala
Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu1 5 10 15Leu Gly Ile Trp Gly
Gly Ser Gly Lys Leu Ile Cys Cys Thr Thr Ala20 25 30Val Pro Trp Asn
Ser Ser Trp Ser Asn Lys Leu Asp Arg Ile Trp Asn35 40 45Asn Met Thr
Trp Leu Glu50682PRTArtificial sequenceChimera HIV gP120 V3-V3 6Ile
Gly Cys Thr Arg Pro Ser Asn Asn Thr Arg Lys Gly Ile His Met1 5 10
15Gly Pro Gly Gly Ala Phe Tyr Thr Thr Gly Gln Ile Ile Arg Asn Ile20
25 30Arg Gln Ala His Cys Gly Leu Leu Gly Gly Cys Thr Arg Pro Asn
Asn35 40 45Asn Thr Arg Arg Ser Ile His Ile Glu Pro Glu Gly Ala Phe
Tyr Thr50 55 60Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys
Gly Leu Gly65 70 75 80Leu Glu7100PRTArtificial sequenceChimera HIV
gP120-C1-C5-gp41 7Val Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val
Pro Val Trp Lys1 5 10 15Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala
Pro Thr Lys Cys Lys20 25 30Arg Arg Val Val Gln Arg Glu Lys Arg Gly
Gly Gly Gly Gly Gln Ala35 40 45Arg Val Ile Ala Val Glu Arg Tyr Leu
Lys Asp Gln Gln Leu Leu Gly50 55 60Ile Trp Gly Gly Ser Gly Lys Leu
Ile Cys Cys Thr Thr Ala Val Pro65 70 75 80Trp Asn Ser Ser Trp Ser
Asn Lys Leu Asp Arg Ile Trp Asn Asn Met85 90 95Thr Trp Leu
Glu1008264DNAArtificial sequencechimeric gene HIV gP120 V3-V3
8gaattccata tggtcgacat cggttgcacc cgtccgagca acaacacccg taaaggtatc
60cacatgggcc cgggtggtgc tttctacacc accggtcaga tcatccgtaa catccgtcag
120gctcactgtg gtctgctggg tggttgtacc cgtccgaaca acaacacccg
tcgtagcatc 180cacatcgaac cggaaggtgc tttctacacc accggtgaaa
tcatcggtga catccgtcag 240gctcactgtg gcctgggtct cgag
2649318DNAArtificial sequencechimeric gene HIV gP120 C1-C5-gp41
9gaattccata tggtcgacgt tgaaaaactg tgggttaccg tttactacgg tgttccggtt
60tggaaaaaag ttgttaaaat cgaaccgctg ggtgttgctc cgaccaaatg caaacgtcgt
120gttgttcagc gtgaaaagcg tggtggcggt ggcggtcaag ctcgtgttat
cgctgttgaa 180cgttacctga aagaccagca gctgctgggt atctggggtg
gtagcggtaa actgatctgc 240tgcaccaccg ctgttccgtg gaacagcagc
tggagcaaca aactggaccg tatctggaac 300aacatgacct ggctcgag
3181040DNAArtificial sequenceForward primer F1 for chimeric gene
HIV gP120 V3-V3 10catccgtaac atccgtcagg ctcactgtgg tctgctgggt
401139DNAArtificial sequenceForward primer F2 for chimeric gene HIV
gP120 V3-V3 11gctttctaca ccaccggtca gatcatccgt aacatccgt
391242DNAArtificial sequenceForward primer F3 for chimeric gene HIV
gP120 V3-V3 12aaaggtatcc acatgggccc gggtggtgct ttctacacca cc
421339DNAArtificial sequenceForward primer F4 for chimeric gene HIV
gP120 V3-V3 13acccgtccga gcaacaacac ccgtaaaggt atccacatg
391446DNAArtificial sequenceForward primer F5 for chimeric gene HIV
gP120 V3-V3 14cccgaattcc atatggtcga catcggttgc acccgtccga gcaaca
461539DNAArtificial sequenceReverse primer R1 for chimeric gene HIV
gP120 V3-V3 15gttgttgttc ggacgggtac aaccacccag cagaccaca
391639DNAArtificial sequenceReverse primer R2 for chimeric gene HIV
gP120 V3-V3 16ttcgatgtgg atgctacgac gggtgttgtt gttaggacg
391739DNAArtificial sequenceReverse primer R3 for chimeric gene HIV
gP120 V3-V3 17ggtggtgtag aaagcacctt ccggttcgat gtggatgct
391839DNAArtificial sequenceReverse primer R4 for chimeric gene HIV
gP120 V3-V3 18acggatatca ccgatgattt caccggtggt gtagaaagc
391964DNAArtificial sequenceReverse primer R5 for chimeric gene HIV
gP120 V3-V3 19aaaaaaattc ccattatttt tctcgagacc caggccacag
tgagcctgac ggatgtcacc 60gatg 642044DNAArtificial sequenceForward
primer F1 for chimeric gene HIV gP120 C1-C4-gp41 20gttgctccga
ccaaatgcaa acgtcgtgtt gttcagcgtg aaaa 442147DNAArtificial
sequenceForward primer F2 for chimeric gene HIV gP120 C1-C4-gp41
21aaaaaagttg ttaaaatcga accgctgggt gttgctccga ccaaatg
472249DNAArtificial sequenceForward primer F3 for chimeric gene HIV
gP120 C1-C4-gp41 22gtgggttacc gtttactacg gtgttccggt ttggaaaaaa
gttgttaaa 492349DNAArtificial sequenceForward primer F4 for
chimeric gene HIV gP120 C1-C4-gp41 23cccgaattcc atatggtcga
cgttgaaaaa ctgtgggtta ccgtttact 492454DNAArtificial sequenceReverse
primer R1 for chimeric gene HIV gP120 C1-C4-gp41 24aacagcgata
acacgagctt gaccgccacc gccaccacgc ttttcacgct gaac
542546DNAArtificial sequenceReverse primer R2 for chimeric gene HIV
gP120 C1-C4-gp41 25cagcagctgc tggtctttca ggtaacgttc aacagcgata
acacga 462644DNAArtificial sequenceReverse primer R3 for chimeric
gene HIV gP120 C1-C4-gp41 26gatcagttta ccgctaccac cccagatacc
cagcagctgc tggt 442745DNAArtificial sequenceReverse primer R4 for
chimeric gene HIV gP120 C1-C4-gp41 27gctgttccac ggaacagcgg
tggtgcagca gatcagttta ccgct 452844DNAArtificial sequenceReverse
primer R5 for chimeric gene HIV gP120 C1-C4-gp41 28ccagatacgg
tccagtttgt tgctccagct gctgttccac ggaa 442939DNAArtificial
sequenceReverse primer R6 for chimeric gene HIV gP120 C1-C4-gp41
29aaactcgagc caggtcatgt tgttccagat acggtccag 39304PRTArtificial
sequenceCarboxyl terminal moiety KDEL 30Lys Asp Glu
Leu13198PRTArtificial sequenceChimera for HIV subtype A gp120
C1-C5-gp41 31Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro
Ile Trp Lys1 5 10 15Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro
Thr Lys Ala Arg20 25 30Arg Arg Val Val Glu Arg Glu Lys Arg Gly Gly
Gly Gly Gly Gln Ala35 40 45Arg Val Leu Ala Val Glu Arg Tyr Leu Arg
Asp Gln Gln Leu Leu Gly50 55 60Ile Trp Gly Cys Ser Gly Lys Leu Ile
Cys Pro Thr Asn Val Pro Trp65 70 75 80Asn Ser Ser Trp Ser Asn Lys
Ser Leu Asp Glu Ile Trp Glu Asn Met85 90 95Thr Trp3298PRTArtificial
sequenceChimera for HIV subtype B gp120 C1-C5-gp41 32Thr Glu Lys
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys1 5 10 15Lys Val
Val Lys Ile Glu Pro Leu Gly Ile Ala Pro Thr Lys Ala Lys20 25 30Arg
Arg Val Val Gln Arg Glu Lys Arg Gly Gly Gly Gly Gly Gln Ala35 40
45Arg Val Leu Ala Leu Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly50
55 60Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Thr Val Pro
Trp65 70 75 80Asn Ala Ser Trp Ser Asn Arg Ser Leu Asp Glu Ile Trp
Asp Asn Met85 90 95Thr Trp33100PRTArtificial sequenceChimera for
HIV subtype C gp120 C1-C5-gp41 33Met Gly Asn Leu Trp Val Thr Val
Tyr Tyr Gly Val Pro Val Trp Lys1 5 10 15Lys Tyr Lys Val Val Glu Ile
Lys Pro Leu Gly Val Ala Pro Thr Lys20 25 30Pro Lys Arg Arg Val Val
Glu Arg Glu Lys Arg Gly Gly Gly Gly Gly35 40 45Gln Thr Arg Val Leu
Ala Ile Glu Arg His Leu Arg Asp Gln Gln Leu50 55 60Leu Gly Ile Trp
Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val65 70 75 80Pro Trp
Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Glu Ile Trp Asp85 90 95Asn
Met Thr Trp1003498PRTArtificial sequenceChimera for HIV subtype D
gp120 C1-C5-gp41 34Ala Asp Asn Leu Trp Val Thr Val Tyr Tyr Gly Val
Pro Val Trp Lys1 5 10 15Lys Val Val Gln Ile Glu Pro Leu Gly Val Ala
Pro Thr Arg Ala Lys20 25 30Arg Arg Val Val Glu Arg Glu Lys Arg Gly
Gly Gly Gly Gly Gln Ala35 40 45Arg Ile Leu Ala Val Glu Arg Tyr Leu
Lys Asp Gln Gln Leu Leu Gly50 55 60Ile Trp Gly Cys Ser Gly Lys His
Ile Cys Thr Thr Asn Val Pro Trp65 70 75 80Asn Ser Ser Trp Ser Asn
Arg Ser Leu Asn Glu Ile Trp Gln Asn Met85 90 95Thr
Trp3598PRTArtificial sequenceChimera for HIV subtype E gp120
C1-C5-gp41 35Ser Xaa Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro
Val Trp Arg1 5 10 15Lys Val Val Gln Ile Glu Pro Leu Gly Ile Ala Pro
Thr Arg Pro Lys20 25 30Arg Arg Val Val Glu Arg Glu Lys Arg Gly Gly
Gly Gly Gly Gln Ala35 40 45Arg Val Leu Ala Val Glu Arg Tyr Leu Lys
Asp Gln Lys Phe Leu Gly50 55 60Leu Trp Gly Cys Ser Gly Lys Ile Ile
Cys Thr Thr Ala Val Pro Trp65 70 75 80Asn Ser Ser Trp Ser Asn Arg
Ser Phe Glu Glu Ile Trp Asn Asn Met85 90 95Thr Trp3698PRTArtificial
sequenceChimera for HIV subtype F gp120 C1-C5-gp41 36Ala Asp Asn
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys1 5 10 15Lys Val
Val Glu Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys20 25 30Arg
Gln Val Val Gln Arg Glu Lys Arg Gly Gly Gly Gly Gly Gln Ala35 40
45Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly50
55 60Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro
Trp65 70 75 80Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Glu Ile Trp
Asn Asn Met85 90 95Thr Trp37102PRTArtificial sequenceChimera for
HIV subtype G gp120 C1-C5-gp41 37Ala Ser Asn Asn Leu Trp Val Thr
Val Tyr Tyr Gly Val Pro Val Trp1 5 10 15Glu Asp Ala Lys Lys Val Val
Lys Ile Lys Pro Leu Gly Val Ala Pro20 25 30Thr Lys Ala Arg Arg Arg
Val Val Gly Arg Glu Lys Arg Gly Gly Gly35 40 45Gly Gly Gln Ala Arg
Val Leu Ala Leu Glu Arg Tyr Leu Arg Asp Gln50 55 60Gln Leu Leu Gly
Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr65 70 75 80Asn Val
Pro Trp Asn Ala Ser Trp Ser Asn Lys Thr Tyr Asn Asp Ile85 90 95Trp
Asp Asn Met Thr Trp10038102PRTArtificial sequenceChimera for HIV
subtype H gp120 C1-C5-gp41 38Val Val Gly Asn Leu Trp Val Thr Val
Tyr Tyr Gly Val Pro Val Trp1 5 10 15Lys Lys Val Val Lys Ile Glu Pro
Leu Gly Val Ala Pro Thr Glu Ala20 25 30Arg Arg Arg Val Val Glu Arg
Glu Lys Arg Gly Gly Gly Gly Gly Gln35 40 45Ala Arg Val Leu Ala Val
Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu50 55 60Gly Ile Trp Gly Cys
Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro65 70 75 80Trp Asn Ser
Ser Trp Ser Asn Lys Ser Leu Asp Glu Ile Trp Asp Asn85 90 95Met Thr
Trp Met Glu Trp1003999PRTArtificial sequenceChimera for HIV subtype
J gp120 C1-C5-gp41 39Ala Lys Glu Asp Leu Trp Val Thr Val Tyr Tyr
Gly Val Pro Val Trp1 5 10 15Lys Lys Val Val Glu Ile Glu Pro Leu Gly
Val Ala Pro Thr Lys Ala20 25 30Lys Arg Arg Val Val Glu Arg Glu Lys
Arg Gly Gly Gly Gly Gly Gln35 40 45Ala Arg Val Leu Ala Val Glu Arg
Tyr Leu Lys Asp Gln Gln Leu Leu50 55 60Gly Ile Trp Gly Cys Ser Gly
Lys Leu Ile Cys Thr Thr Asn Val Pro65 70 75 80Trp Asn Ala Ser Trp
Ser Asn Lys Ser Tyr Glu Asp Ile Trp Glu Asn85 90 95Met Thr
Trp4099PRTArtificial sequenceChimera for HIV subtype K gp120
C1-C5-gp41 40Ala Ala Asn Asn Leu Trp Val Thr Val Tyr Tyr Gly Val
Pro Val Trp1 5 10 15Lys Lys Val Val Gln Ile Glu Pro Leu Gly Ile Ala
Pro Thr Arg Ala20 25 30Arg Arg Arg Val Val Gln Arg Glu Lys Arg Gly
Gly Gly Gly Gly Arg35 40 45Ala Arg Val Leu Ala Val Glu Arg Tyr Leu
Arg Asp Gln Gln Leu Leu50 55 60Gly Ile Trp Gly Cys Ser Gly Lys Leu
Ile Cys Thr Thr Asn Val Pro65 70 75 80Trp Asn Ser Ser Trp Ser Asn
Lys Ser Gln Ser Glu Ile Trp Glu Asn85 90 95Met Thr
Trp41294DNAArtificial sequenceChimeric gene for HIV subtype A gp120
C1-C5-gp41 41gctgaaaacc tgtgggttac cgtttactac ggtgttccaa tctggaaaaa
agttgttaaa 60atcgaaccac tgggtgttgc tccaaccaaa gctcgtcgtc gtgttgttga
acgtgaaaaa 120cgtggtggtg gtggtggtca ggctcgtgtt ctggctgttg
aacgttacct gcgtgaccag 180cagctgctgg gtatctgggg ttgttccggt
aaactgatct gtccaaccaa cgttccatgg 240aactcctcct ggtccaacaa
atccctggac gaaatctggg aaaacatgac ctgg 29442294DNAArtificial
sequenceChimeric gene for HIV subtype B gp120 C1-C5-gp41
42accgaaaaac tgtgggttac cgtttactac ggtgttccag tgtggaaaaa agttgttaaa
60atcgaaccac tgggtatcgc tccaaccaaa gctaaacgtc gtgttgttca gcgtgaaaaa
120cgtggtggtg gtggtggtca ggctcgtgtt ctggctctgg aacgttacct
gcgtgaccag 180cagctgctgg gtatctgggg ttgttccggt aaactgatct
gtaccaccac cgttccatgg 240aacgcttcct ggtccaaccg ttccctggac
gaaatctggg acaacatgac ctgg 29443300DNAArtificial sequenceChimeric
gene for HIV subtype C gp120 C1-C5-gp41 43atgggtaacc tgtgggttac
cgtttactac ggtgttccag tgtggaaaaa atacaaagtt 60gttgaaatca aaccactggg
tgttgctcca accaaaccaa aacgtcgtgt tgttgaacgt 120gaaaaacgtg
gtggtggtgg tggtcagacc cgtgttctgg ctatcgaacg tcacctgcgt
180gaccagcagc tgctgggtat ctggggttgt tccggtaaac tgatctgtac
caccgctgtt 240ccatggaact cctcctggtc caacaaatcc caggaagaaa
tctgggacaa catgacctgg 30044294DNAArtificial sequenceChimeric gene
for HIV subtype D gp120 C1-C5-gp41 44gctgacaacc tgtgggttac
cgtttactac ggtgttccag tgtggaaaaa agttgttcag 60atcgaaccac tgggtgttgc
tccaacccgt gctaaacgtc gtgttgttga acgtgaaaaa 120cgtggtggtg
gtggtggtca ggctcgtatt ctggctgttg aacgttacct gaaagaccag
180cagctgctgg gtatctgggg ttgttccggt aaacacatct gtaccaccaa
cgttccatgg 240aactcctcct ggtccaaccg ttccctgaac gaaatctggc
agaacatgac ctgg 29445294DNAArtificial sequenceChimeric gene for HIV
subtype E gp120 C1-C5-gp41 45tccaacaacc tgtgggttac cgtttactac
ggtgttccag tgtggcgtaa agttgttcag 60atcgaaccac tgggtatcgc tccaacccgt
ccaaaacgtc gtgttgttga acgtgaaaaa 120cgtggtggtg gtggtggtca
ggctcgtgtt ctggctgttg aacgttacct gaaagaccag 180aaattcctgg
gtctgtgggg ttgttccggt aaaatcatct gtaccaccgc tgttccatgg
240aactcctcct ggtccaaccg ttccttcgaa gaaatctgga acaacatgac ctgg
29446294DNAArtificial sequenceChimeric gene for HIV subtype F gp120
C1-C5-gp41 46gctgacaacc tgtgggttac cgtttactac ggtgttccag tgtggaaaaa
agttgttgaa 60atcgaaccac tgggtgttgc tccaaccaaa gctaaacgtc aggttgttca
gcgtgaaaaa 120cgtggtggtg gtggtggtca ggctcgtgtt ctggctgttg
aacgttacct gaaagaccag 180cagctgctgg gtatctgggg ttgttccggt
aaactgatct gtaccaccaa cgttccatgg 240aactcctcct ggtccaacaa
atcccaggaa gaaatctgga acaacatgac ctgg 29447306DNAArtificial
sequenceChimeric gene for HIV subtype G gp120 C1-C5-gp41
47gcttccaaca acctgtgggt taccgtttac tacggtgttc cagtgtggga agacgctaaa
60aaagttgtta aaatcaaacc actgggtgtt gctccaacca aagctcgtcg tcgtgttgtt
120ggtcgtgaaa aacgtggtgg tggtggtggt caggctcgtg ttctggctct
ggaacgttac 180ctgcgtgacc agcagctgct gggtatctgg ggttgttccg
gtaaactgat ctgtaccacc 240aacgttccat ggaacgcttc ctggtccaac
aaaacctaca acgacatctg ggacaacatg 300acctgg 30648306DNAArtificial
sequenceChimeric gene for HIV subtype H gp120 C1-C5-gp41
48gttgttggta acctgtgggt taccgtttac tacggtgttc cagtgtggaa aaaagttgtt
60aaaatcgaac cactgggtgt tgctccaacc gaagctcgtc gtcgtgttgt tgaacgtgaa
120aaacgtggtg gtggtggtgg tcaggctcgt gttctggctg ttgaacgtta
cctgaaagac 180cagcagctgc tgggtatctg gggttgttcc ggtaaactga
tctgtaccac caacgttcca 240tggaactcct cctggtccaa
caaatccctg gacgaaatct gggacaacat gacctggatg 300gaatgg
30649297DNAArtificial sequenceChimeric gene for HIV subtype J gp120
C1-C5-gp41 49gctaaagaag acctgtgggt taccgtttac tacggtgttc cagtgtggaa
aaaagttgtt 60gaaatcgaac cactgggtgt tgctccaacc aaagctaaac gtcgtgttgt
tgaacgtgaa 120aaacgtggtg gtggtggtgg tcaggctcgt gttctggctg
ttgaacgtta cctgaaagac 180cagcagctgc tgggtatctg gggttgttcc
ggtaaactga tctgtaccac caacgttcca 240tggaacgctt cctggtccaa
caaatcctac gaagacatct gggaaaacat gacctgg 29750297DNAArtificial
sequenceChimeric gene for HIV subtype K gp120 C1-C5-gp41
50gctgctaaca acctgtgggt taccgtttac tacggtgttc cagtgtggaa aaaagttgtt
60cagatcgaac cactgggtat cgctccaacc cgtgctcgtc gtcgtgttgt tcagcgtgaa
120aaacgtggtg gtggtggtgg tcgtgctcgt gttctggctg ttgaacgtta
cctgcgtgac 180cagcagctgc tgggtatctg gggttgttcc ggtaaactga
tctgtaccac caacgttcca 240tggaactcct cctggtccaa caaatcccag
tccgaaatct gggaaaacat gacctgg 2975145DNAArtificial sequenceForward
primer F1 for chimeric gene HIV subtype A gp120 C1-C5-gp41
51gttgaacgtg aaaaacgtgg tggtggtggt ggtcaggctc gtgtt
455245DNAArtificial sequenceForward primer F2 for chimeric gene HIV
subtype A gp120 C1-C5-gp41 52gttgctccaa ccaaagctcg tcgtcgtgtt
gttgaacgtg aaaaa 455349DNAArtificial sequenceForward primer F3 for
chimeric gene HIV subtype A gp120 C1-C5-gp41 53ctggaaaaaa
gttgttaaaa tcgaaccact gggtgttgct ccaaccaaa 495446DNAArtificial
sequenceForward primer F4 for chimeric gene HIV subtype A gp120
C1-C5-gp41 54gtgggttacc gtttactacg gtgttccaat ctggaaaaaa gttgtt
465547DNAArtificial sequenceForward primer F5 for chimeric gene HIV
subtype A gp120 C1-C5-gp41 55cccgaattcc atatggtcga cgctgaaaac
ctgtgggtta ccgttta 475647DNAArtificial sequenceReverse primer R1
for chimeric gene HIV subtype A gp120 C1-C5-gp41 56tgctggtcac
gcaggtaacg ttcaacagcc agaacacgag cctgacc 475746DNAArtificial
sequenceReverse primer R2 for chimeric gene HIV subtype A gp120
C1-C5-gp41 57tttaccggaa caaccccaga tacccagcag ctgctggtca cgcagg
465846DNAArtificial sequenceReverse primer R3 for chimeric gene HIV
subtype A gp120 C1-C5-gp41 58agttccatgg aacgttggtt ggacagatca
gtttaccgga acaacc 465948DNAArtificial sequenceReverse primer R4 for
chimeric gene HIV subtype A gp120 C1-C5-gp41 59tttcgtccag
ggatttgttg gaccaggagg agttccatgg aacgttgg 486049DNAArtificial
sequenceReverse primer R5 for chimeric gene HIV subtype A gp120
C1-C5-gp41 60atttttctcg agccaggtca tgttttccca gatttcgtcc agggatttg
496145DNAArtificial sequenceForward primer F1 for chimeric gene HIV
subtype B gp120 C1-C5-gp41 61gttcagcgtg aaaaacgtgg tggtggtggt
ggtcaggctc gtgtt 456246DNAArtificial sequenceForward primer F2 for
chimeric gene HIV subtype B gp120 C1-C5-gp41 62atcgctccaa
ccaaagctaa acgtcgtgtt gttcagcgtg aaaaac 466349DNAArtificial
sequenceForward primer F3 for chimeric gene HIV subtype B gp120
C1-C5-gp41 63gtggaaaaaa gttgttaaaa tcgaaccact gggtatcgct ccaaccaaa
496446DNAArtificial sequenceForward primer F4 for chimeric gene HIV
subtype B gp120 C1-C5-gp41 64gtgggttacc gtttactacg gtgttccagt
gtggaaaaaa gttgtt 466547DNAArtificial sequenceForward primer F5 for
chimeric gene HIV subtype B gp120 C1-C5-gp41 65cccgaattcc
atatggtcga caccgaaaaa ctgtgggtta ccgttta 476647DNAArtificial
sequenceReverse primer R1 for chimeric gene HIV subtype B gp120
C1-C5-gp41 66tgctggtcac gcaggtaacg ttccagagcc agaacacgag cctgacc
476746DNAArtificial sequenceReverse primer R2 for chimeric gene HIV
subtype B gp120 C1-C5-gp41 67tttaccggaa caaccccaga tacccagcag
ctgctggtca cgcagg 466848DNAArtificial sequenceReverse primer R3 for
chimeric gene HIV subtype B gp120 C1-C5-gp41 68agcgttccat
ggaacggtgg tggtacagat cagtttaccg gaacaacc 486944DNAArtificial
sequenceReverse primer R4 for chimeric gene HIV subtype B gp120
C1-C5-gp41 69atttcgtcca gggaacggtt ggaccaggaa gcgttccatg gaac
447047DNAArtificial sequenceReverse primer R5 for chimeric gene HIV
subtype B gp120 C1-C5-gp41 70atttttctcg agccaggtca tgttgtccca
gatttcgtcc agggaac 477145DNAArtificial sequenceForward primer F1
for chimeric gene HIV subtype C gp120 C1-C5-gp41 71gttgaacgtg
aaaaacgtgg tggtggtggt ggtcagaccc gtgtt 457245DNAArtificial
sequenceForward primer F2 for chimeric gene HIV subtype C gp120
C1-C5-gp41 72gttgctccaa ccaaaccaaa acgtcgtgtt gttgaacgtg aaaaa
457352DNAArtificial sequenceForward primer F3 for chimeric gene HIV
subtype C gp120 C1-C5-gp41 73aaaaaataca aagttgttga aatcaaacca
ctgggtgttg ctccaaccaa ac 527451DNAArtificial sequenceForward primer
F4 for chimeric gene HIV subtype C gp120 C1-C5-gp41 74ctgtgggtta
ccgtttacta cggtgttcca gtgtggaaaa aatacaaagt t 517545DNAArtificial
sequenceForward primer F5 for chimeric gene HIV subtype C gp120
C1-C5-gp41 75cccgaattcc atatggtcga catgggtaac ctgtgggtta ccgtt
457647DNAArtificial sequenceReverse primer R1 for chimeric gene HIV
subtype C gp120 C1-C5-gp41 76tgctggtcac gcaggtgacg ttcgatagcc
agaacacggg tctgacc 477746DNAArtificial sequenceReverse primer R2
for chimeric gene HIV subtype C gp120 C1-C5-gp41 77tttaccggaa
caaccccaga tacccagcag ctgctggtca cgcagg 467846DNAArtificial
sequenceReverse primer R3 for chimeric gene HIV subtype C gp120
C1-C5-gp41 78agttccatgg aacagcggtg gtacagatca gtttaccgga acaacc
467948DNAArtificial sequenceReverse primer R4 for chimeric gene HIV
subtype C gp120 C1-C5-gp41 79tttcttcctg ggatttgttg gaccaggagg
agttccatgg aacagcgg 488049DNAArtificial sequenceReverse primer R5
for chimeric gene HIV subtype C gp120 C1-C5-gp41 80atttttctcg
agccaggtca tgttgtccca gatttcttcc tgggatttg 498145DNAArtificial
sequenceForward primer F1 for chimeric gene HIV subtype D gp120
C1-C5-gp41 81gttgaacgtg aaaaacgtgg tggtggtggt ggtcaggctc gtatt
458245DNAArtificial sequenceForward primer F2 for chimeric gene HIV
subtype D gp120 C1-C5-gp41 82gttgctccaa cccgtgctaa acgtcgtgtt
gttgaacgtg aaaaa 458349DNAArtificial sequenceForward primer F3 for
chimeric gene HIV subtype D gp120 C1-C5-gp41 83gtggaaaaaa
gttgttcaga tcgaaccact gggtgttgct ccaacccgt 498446DNAArtificial
sequenceForward primer F4 for chimeric gene HIV subtype D gp120
C1-C5-gp41 84gtgggttacc gtttactacg gtgttccagt gtggaaaaaa gttgtt
468547DNAArtificial sequenceForward primer F5 for chimeric gene HIV
subtype D gp120 C1-C5-gp41 85cccgaattcc atatggtcga cgctgacaac
ctgtgggtta ccgttta 478647DNAArtificial sequenceReverse primer R1
for chimeric gene HIV subtype D gp120 C1-C5-gp41 86tgctggtctt
tcaggtaacg ttcaacagcc agaatacgag cctgacc 478746DNAArtificial
sequenceReverse primer R2 for chimeric gene HIV subtype D gp120
C1-C5-gp41 87tttaccggaa caaccccaga tacccagcag ctgctggtct ttcagg
468846DNAArtificial sequenceReverse primer R3 for chimeric gene HIV
subtype D gp120 C1-C5-gp41 88agttccatgg aacgttggtg gtacagatgt
gtttaccgga acaacc 468948DNAArtificial sequenceReverse primer R4 for
chimeric gene HIV subtype D gp120 C1-C5-gp41 89tttcgttcag
ggaacggttg gaccaggagg agttccatgg aacgttgg 489049DNAArtificial
sequenceReverse primer R5 for chimeric gene HIV subtype D gp120
C1-C5-gp41 90atttttctcg agccaggtca tgttctgcca gatttcgttc agggaacgg
499145DNAArtificial sequenceForward primer F1 for chimeric gene HIV
subtype E gp120 C1-C5-gp41 91gttgaacgtg aaaaacgtgg tggtggtggt
ggtcaggctc gtgtt 459246DNAArtificial sequenceForward primer F2 for
chimeric gene HIV subtype E gp120 C1-C5-gp41 92atcgctccaa
cccgtccaaa acgtcgtgtt gttgaacgtg aaaaac 469349DNAArtificial
sequenceForward primer F3 for chimeric gene HIV subtype E gp120
C1-C5-gp41 93gtggcgtaaa gttgttcaga tcgaaccact gggtatcgct ccaacccgt
499446DNAArtificial sequenceForward primer F4 for chimeric gene HIV
subtype E gp120 C1-C5-gp41 94gtgggttacc gtttactacg gtgttccagt
gtggcgtaaa gttgtt 469544DNAArtificial sequenceForward primer F5 for
chimeric gene HIV subtype E gp120 C1-C5-gp41 95cccgaattcc
atatggtcga ctccaacctg tgggttaccg ttta 449648DNAArtificial
sequenceReverse primer R1 for chimeric gene HIV subtype E gp120
C1-C5-gp41 96tttctggtct ttcaggtaac gttcaacagc cagaacacga gcctgacc
489746DNAArtificial sequenceReverse primer R2 for chimeric gene HIV
subtype E gp120 C1-C5-gp41 97tttaccggaa caaccccaca gacccaggaa
tttctggtct ttcagg 469846DNAArtificial sequenceReverse primer R3 for
chimeric gene HIV subtype E gp120 C1-C5-gp41 98agttccatgg
aacagcggtg gtacagatga ttttaccgga acaacc 469948DNAArtificial
sequenceReverse primer R4 for chimeric gene HIV subtype E gp120
C1-C5-gp41 99tttcttcgaa ggaacggttg gaccaggagg agttccatgg aacagcgg
4810049DNAArtificial sequenceReverse primer R5 for chimeric gene
HIV subtype E gp120 C1-C5-gp41 100atttttctcg agccaggtca tgttgttcca
gatttcttcg aaggaacgg 4910145DNAArtificial sequenceForward primer F1
for chimeric gene HIV subtype F gp120 C1-C5-gp41 101gttcagcgtg
aaaaacgtgg tggtggtggt ggtcaggctc gtgtt 4510245DNAArtificial
sequenceForward primer F2 for chimeric gene HIV subtype F gp120
C1-C5-gp41 102gttgctccaa ccaaagctaa acgtcaggtt gttcagcgtg aaaaa
4510349DNAArtificial sequenceForward primer F3 for chimeric gene
HIV subtype F gp120 C1-C5-gp41 103gtggaaaaaa gttgttgaaa tcgaaccact
gggtgttgct ccaaccaaa 4910446DNAArtificial sequenceForward primer F4
for chimeric gene HIV subtype F gp120 C1-C5-gp41 104gtgggttacc
gtttactacg gtgttccagt gtggaaaaaa gttgtt 4610547DNAArtificial
sequenceForward primer F5 for chimeric gene HIV subtype F gp120
C1-C5-gp41 105cccgaattcc atatggtcga cgctgacaac ctgtgggtta ccgttta
4710647DNAArtificial sequenceReverse primer R1 for chimeric gene
HIV subtype F gp120 C1-C5-gp41 106tgctggtctt tcaggtaacg ttcaacagcc
agaacacgag cctgacc 4710746DNAArtificial sequenceReverse primer R2
for chimeric gene HIV subtype F gp120 C1-C5-gp41 107tttaccggaa
caaccccaga tacccagcag ctgctggtct ttcagg 4610846DNAArtificial
sequenceReverse primer R3 for chimeric gene HIV subtype F gp120
C1-C5-gp41 108agttccatgg aacgttggtg gtacagatca gtttaccgga acaacc
4610948DNAArtificial sequenceReverse primer R4 for chimeric gene
HIV subtype F gp120 C1-C5-gp41 109tttcttcctg ggatttgttg gaccaggagg
agttccatgg aacgttgg 4811049DNAArtificial sequenceReverse primer R5
for chimeric gene HIV subtype F gp120 C1-C5-gp41 110atttttctcg
agccaggtca tgttgttcca gatttcttcc tgggatttg 4911145DNAArtificial
sequenceForward primer F1 for chimeric gene HIV subtype G gp120
C1-C5-gp41 111gttggtcgtg aaaaacgtgg tggtggtggt ggtcaggctc gtgtt
4511245DNAArtificial sequenceForward primer F2 for chimeric gene
HIV subtype G gp120 C1-C5-gp41 112gttgctccaa ccaaagctcg tcgtcgtgtt
gttggtcgtg aaaaa 4511351DNAArtificial sequenceForward primer F3 for
chimeric gene HIV subtype G gp120 C1-C5-gp41 113gacgctaaaa
aagttgttaa aatcaaacca ctgggtgttg ctccaaccaa a 5111452DNAArtificial
sequenceForward primer F4 for chimeric gene HIV subtype G gp120
C1-C5-gp41 114gtgggttacc gtttactacg gtgttccagt gtgggaagac
gctaaaaaag tt 5211550DNAArtificial sequenceForward primer F5 for
chimeric gene HIV subtype G gp120 C1-C5-gp41 115cccgaattcc
atatggtcga cgcttccaac aacctgtggg ttaccgttta 5011647DNAArtificial
sequenceReverse primer R1 for chimeric gene HIV subtype G gp120
C1-C5-gp41 116tgctggtcac gcaggtaacg ttccagagcc agaacacgag cctgacc
4711746DNAArtificial sequenceReverse primer R2 for chimeric gene
HIV subtype G gp120 C1-C5-gp41 117tttaccggaa caaccccaga tacccagcag
ctgctggtca cgcagg 4611848DNAArtificial sequenceReverse primer R3
for chimeric gene HIV subtype G gp120 C1-C5-gp41 118agcgttccat
ggaacgttgg tggtacagat cagtttaccg gaacaacc 4811944DNAArtificial
sequenceReverse primer R4 for chimeric gene HIV subtype G gp120
C1-C5-gp41 119atgtcgttgt aggttttgtt ggaccaggaa gcgttccatg gaac
4412046DNAArtificial sequenceReverse primer R5 for chimeric gene
HIV subtype G gp120 C1-C5-gp41 120tttctcgagc caggtcatgt tgtcccagat
gtcgttgtag gttttg 4612145DNAArtificial sequenceForward primer F1
for chimeric gene HIV subtype H gp120 C1-C5-gp41 121gttgaacgtg
aaaaacgtgg tggtggtggt ggtcaggctc gtgtt 4512245DNAArtificial
sequenceForward primer F2 for chimeric gene HIV subtype H gp120
C1-C5-gp41 122gttgctccaa ccgaagctcg tcgtcgtgtt gttgaacgtg aaaaa
4512349DNAArtificial sequenceForward primer F3 for chimeric gene
HIV subtype H gp120 C1-C5-gp41 123gtggaaaaaa gttgttaaaa tcgaaccact
gggtgttgct ccaaccgaa 4912448DNAArtificial sequenceForward primer F4
for chimeric gene HIV subtype H gp120 C1-C5-gp41 124ctgtgggtta
ccgtttacta cggtgttcca gtgtggaaaa aagttgtt 4812548DNAArtificial
sequenceForward primer F5 for chimeric gene HIV subtype H gp120
C1-C5-gp41 125cccgaattcc atatggtcga cgttgttggt aacctgtggg ttaccgtt
4812647DNAArtificial sequenceReverse primer R1 for chimeric gene
HIV subtype H gp120 C1-C5-gp41 126tgctggtctt tcaggtaacg ttcaacagcc
agaacacgag cctgacc 4712746DNAArtificial sequenceReverse primer R2
for chimeric gene HIV subtype H gp120 C1-C5-gp41 127tttaccggaa
caaccccaga tacccagcag ctgctggtct ttcagg 4612846DNAArtificial
sequenceReverse primer R3 for chimeric gene HIV subtype H gp120
C1-C5-gp41 128agttccatgg aacgttggtg gtacagatca gtttaccgga acaacc
4612948DNAArtificial sequenceReverse primer R4 for chimeric gene
HIV subtype H gp120 C1-C5-gp41 129tttcgtccag ggatttgttg gaccaggagg
agttccatgg aacgttgg 4813055DNAArtificial sequenceReverse primer R5
for chimeric gene HIV subtype H gp120 C1-C5-gp41 130tttctcgagc
cattccatcc aggtcatgtt gtcccagatt tcgtccaggg atttg
5513145DNAArtificial sequenceForward primer F1 for chimeric gene
HIV subtype J gp120 C1-C5-gp41 131gttgaacgtg aaaaacgtgg tggtggtggt
ggtcaggctc gtgtt 4513245DNAArtificial sequenceForward primer F2 for
chimeric gene HIV subtype J gp120 C1-C5-gp41 132gttgctccaa
ccaaagctaa acgtcgtgtt gttgaacgtg aaaaa 4513349DNAArtificial
sequenceForward primer F3 for chimeric gene HIV subtype J gp120
C1-C5-gp41 133gtggaaaaaa gttgttgaaa tcgaaccact gggtgttgct ccaaccaaa
4913448DNAArtificial sequenceForward primer F4 for chimeric gene
HIV subtype J gp120 C1-C5-gp41 134ctgtgggtta ccgtttacta cggtgttcca
gtgtggaaaa aagttgtt 4813548DNAArtificial sequenceForward primer F5
for chimeric gene HIV subtype J gp120 C1-C5-gp41 135cccgaattcc
atatggtcga cgctaaagaa gacctgtggg ttaccgtt 4813647DNAArtificial
sequenceReverse primer R1 for chimeric gene HIV subtype J gp120
C1-C5-gp41 136tgctggtctt tcaggtaacg ttcaacagcc agaacacgag cctgacc
4713746DNAArtificial sequenceReverse primer R2 for chimeric gene
HIV subtype J gp120 C1-C5-gp41 137tttaccggaa caaccccaga tacccagcag
ctgctggtct ttcagg 4613848DNAArtificial sequenceReverse primer R3
for chimeric gene HIV subtype J gp120 C1-C5-gp41 138agcgttccat
ggaacgttgg tggtacagat cagtttaccg gaacaacc 4813944DNAArtificial
sequenceReverse primer R4 for chimeric gene HIV subtype J gp120
C1-C5-gp41 139atgtcttcgt aggatttgtt ggaccaggaa gcgttccatg gaac
4414046DNAArtificial sequenceReverse primer R5 for chimeric gene
HIV subtype J gp120 C1-C5-gp41 140tttctcgagc caggtcatgt tttcccagat
gtcttcgtag gatttg 4614145DNAArtificial sequenceForward primer F1
for chimeric gene HIV subtype K gp120 C1-C5-gp41 141gttcagcgtg
aaaaacgtgg tggtggtggt ggtcgtgctc gtgtt 4514246DNAArtificial
sequenceForward primer F2 for chimeric gene HIV subtype K gp120
C1-C5-gp41 142atcgctccaa cccgtgctcg tcgtcgtgtt gttcagcgtg aaaaac
4614349DNAArtificial sequenceForward primer F3 for chimeric gene
HIV subtype K gp120 C1-C5-gp41 143gtggaaaaaa gttgttcaga tcgaaccact
gggtatcgct ccaacccgt 4914448DNAArtificial sequenceForward primer F4
for chimeric gene HIV subtype K gp120 C1-C5-gp41 144ctgtgggtta
ccgtttacta cggtgttcca gtgtggaaaa aagttgtt 4814548DNAArtificial
sequenceForward primer F5 for chimeric gene HIV subtype K gp120
C1-C5-gp41 145cccgaattcc atatggtcga cgctgctaac
aacctgtggg ttaccgtt 4814647DNAArtificial sequenceReverse primer R1
for chimeric gene HIV subtype K gp120 C1-C5-gp41 146tgctggtcac
gcaggtaacg ttcaacagcc agaacacgag cacgacc 4714746DNAArtificial
sequenceReverse primer R2 for chimeric gene HIV subtype K gp120
C1-C5-gp41 147tttaccggaa caaccccaga tacccagcag ctgctggtca cgcagg
4614846DNAArtificial sequenceReverse primer R3 for chimeric gene
HIV subtype K gp120 C1-C5-gp41 148agttccatgg aacgttggtg gtacagatca
gtttaccgga acaacc 4614948DNAArtificial sequenceReverse primer R4
for chimeric gene HIV subtype K gp120 C1-C5-gp41 149tttcggactg
ggatttgttg gaccaggagg agttccatgg aacgttgg 4815046DNAArtificial
sequenceReverse primer R5 for chimeric gene HIV subtype K gp120
C1-C5-gp41 150tttctcgagc caggtcatgt tttcccagat ttcggactgg gatttg
4615171PRTHuman immunodeficiency virus 151Val Asp Arg Asp Glu Leu
Lys Gly Ile Gly Met Thr Asn Asn Pro Pro1 5 10 15Ile Pro Val Gly Glu
Ile Tyr Lys Arg Trp Ile Ile Leu Gly Leu Asn20 25 30Lys Ile Val Arg
Met Tyr Ser Pro Thr Met Thr Asn Asn Pro Pro Ile35 40 45Pro Val Gly
Glu Ile Tyr Arg Trp Ile Ile Leu Gly Leu Asn Lys Ile50 55 60Val Arg
Met Tyr Ser Pro Thr65 70152132PRTHuman immunodeficiency virus
152Pro Thr Val Arg Gln Arg Met Asp Arg Thr Glu Pro Ala Ala Glu Gly1
5 10 15Val Gly Ala Val Ser Arg Asp Leu Glu Lys His Gly Ala Ile Thr
Ser20 25 30Ser Asn Thr Ala Ala Thr Asn Ala Asp Cys Ala Trp Leu Glu
Ala Gln35 40 45Glu Glu Glu Glu Val Gly Phe Pro Val Arg Pro Gln Val
Pro Leu Arg50 55 60Pro Met Thr Tyr Lys Ala Ala Val Asp Ile Ser His
Phe Leu Lys Glu65 70 75 80Lys Gly Gly Leu Glu Gly Leu Ile Tyr Ser
Gln Lys Arg Gln Glu Ile85 90 95Leu Asp Leu Trp Ile Tyr His Thr Gln
Gly Tyr Phe Pro Asp Trp Gln100 105 110Asn Tyr Thr Pro Gly Pro Gly
Ile Arg Tyr Pro Leu Thr Phe Gly Trp115 120 125Cys Phe Lys
Leu13015366PRTHuman immunodeficiency virus 153Phe Leu Lys Val Pro
Val Asp Pro Glu Gln Val Glu Lys Ala Asn Glu1 5 10 15Gly Asp Asn Asn
Cys Leu Leu His Pro Ile Ser Gln His Gly Met Asp20 25 30Asp Pro Glu
Lys Glu Val Leu Met Trp Lys Phe Asp Ser Arg Leu Ala35 40 45Phe Gln
His Ile Ala Arg Glu Lys His Pro Glu Tyr Tyr Lys Asp Cys50 55 60Leu
Gly65154109PRTHuman immunodeficiency virus 154Arg Asp Glu Leu Lys
Gly Ile Gly Met Glu Pro Val Asp Pro Arg Leu1 5 10 15Glu Pro Trp Lys
His Pro Gly Ser Gln Pro Arg Thr Ala Cys Asn Asn20 25 30Cys Tyr Cys
Lys Lys Cys Cys Phe His Cys Pro Val Cys Phe Ile Ser35 40 45Lys Gly
Leu Gly Ile Ser Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg50 55 60Arg
Ala Pro Gln Asp Ser Glu Thr His Gln Val Ser Leu Ser Lys Gln65 70 75
80Pro Thr Ser Gln Leu Arg Gly Asp Pro Thr Gly Pro Lys Glu Ser Lys85
90 95Lys Lys Val Glu Arg Glu Thr Glu Thr Asp Pro Asn Val100
10515588PRTHuman immunodeficiency virus 155Leu Leu Ala Val Arg Ile
Ile Lys Thr Leu Tyr Gln Ser Asn Pro Tyr1 5 10 15Pro Lys Pro Glu Gly
Tyr Arg Arg Val Arg Arg Asn Arg Arg Arg Arg20 25 30Trp Arg Ala Arg
Gln Arg Gln Ile His Ser Ile Ser Glu Arg Ile Leu35 40 45Ile Thr Cys
Leu Gly Arg Pro Thr Glu Pro Val Pro Leu Gln Leu Pro50 55 60Pro Ile
Glu Arg Leu Asn Ile Asn Cys Ser Glu Ser Gly Gly Thr Ser65 70 75
80Gly Thr Gln Arg Val Gly Asn Pro85156207DNAHuman immunodeficiency
virus 156cgtgacgaac tgaaaggtat cggtatgacc aacaacccgc cgatcccggt
tggtgaaatc 60tacaaacgtt ggatcatcct gggtctgaac aaaatcgttc gtatgtacag
cccgaccatg 120accaacaacc cgccaattcc agtaggcgag atttaccgtt
ggatcatcct gggtctgaac 180aaaatcgttc gcatgtacag cccgact
207157396DNAHuman immunodeficiency virus 157ccgaccgttc gtcagcgtat
ggaccgtacc gaaccggctg ctgagggtgt tggtgctgtt 60agccgtgacc tggaaaaaca
cggtgctatc acctcttcta acaccgctgc taccaacgct 120gactgcgctt
ggctggaagc tcaggaagaa gaagaagttg gttttccggt tcgtccgcag
180gttccgctgc gtccgatgac ctacaaagct gctgttgaca tcagccactt
cctgaaagaa 240aaaggcggcc tggaaggtct gatctacagc cagaaacgtc
aggaaatcct ggacctgtgg 300atctaccaca cccagggcta cttcccggac
tggcagaact acaccccggg tccgggtatc 360cgttacccgc tgaccttcgg
ctggtgtttc aaactg 396158198DNAHuman immunodeficiency virus
158ttcctgaaag ttccggttga cccggaacag gttgaaaaag ctaacgaagg
tgataacaac 60tgcctgctgc acccgatcag ccagcacggt atggacgacc cggaaaaaga
agttctgatg 120tggaaattcg acagccgtct ggctttccag cacatcgctc
gtgaaaaaca cccggaatac 180tacaaagact gcctgggt 198159327DNAHuman
immunodeficiency virus 159cgtgacgaac tgaaaggtat cggtatggaa
ccggttgacc cgcgtctgga accgtggaaa 60cacccgggta gccagccgcg taccgcttgc
aacaactgct actgcaaaaa atgttgtttc 120cactgcccgg tttgctttat
ctctaaaggt ctgggtatca gctacggtcg taaaaagcgt 180cgccagcgcc
gccgcgctcc gcaggactcc gaaacccacc aggttagcct gagcaagcaa
240ccgaccagcc agctgcgtgg tgacccgacc ggtccgaaag aaagcaaaaa
aaaagttgaa 300cgtgaaaccg aaaccgaccc gaacgtt 327160264DNAHuman
immunodeficiency virus 160ctgctggctg ttcgtatcat caaaaccctg
taccagtcta acccgtaccc gaaaccggaa 60ggttaccgtc gtgtccgtcg taaccgccgc
cgccgctggc gtgctcgtca gcgtcagatc 120cactccatca gcgaacgtat
cctgatcacc tgcctgggtc gtccgaccga accggttccg 180ctgcaactgc
cgccgatcga acgtctgaac atcaactgta gcgaaagcgg tggtaccagc
240ggtacccagc gtgttggtaa cccg 26416139DNAArtificial sequenceForward
Primer F1 for gag24 161tggatcatcc tgggtctgaa caaaatcgtt cgtatgtac
3916239DNAArtificial sequenceForward Primer F2 for gag24
162ccggttggtg aaatctacaa acgttggatc atcctgggt 3916341DNAArtificial
sequenceForward Primer F3 for gag24 163tcggtatgac caacaacccg
ccgatcccgg ttggtgaaat c 4116456DNAArtificial sequenceForward Primer
F4 for gag24 164cccgaattcc atatggtcga ccgtgacgaa ctgaaaggta
tcggtatgac caacaa 5616539DNAArtificial sequenceReverse Primer R1
for gag24 165cgggttgttg gtcatggtcg ggctgtacat acgaacgat
3916639DNAArtificial sequenceReverse Primer R2 for gag24
166gtaaatctcg cctactggaa ttggcgggtt gttgttcat 3916740DNAArtificial
sequenceReverse Primer R3 for gag24 167tgttcagacc caggatgatc
caacggtaaa tctcgcctac 4016869DNAArtificial sequenceReverse Primer
R4 for gag24 168aaaaaaattc ccattatttt tctcgagagt cgggctgtac
atgcgaacga ttttgttcag 60acccaggat 6916939DNAArtificial
sequenceForward Primer F1 for nef-N terminus 169gcttggctgg
aagctcagga agaagaagaa gttggtttt 3917040DNAArtificial
sequenceForward Primer F2 for nef-N terminus 170caccgctgct
accaacgctg actgcgcttg gctggaagct 4017142DNAArtificial
sequenceForward Primer F3 for nef-N terminus 171aaacacggtg
ctatcacctc ttctaacacc gctgctacca ac 4217240DNAArtificial
sequenceForward Primer F4 for nef-N terminus 172tggtgctgtt
agccgtgacc tggaaaaaca cggtgctatc 4017342DNAArtificial
sequenceForward Primer F5 for nef-N terminus 173cgtaccgaac
cggctgctga gggtgttggt gctgttagcc gt 4217440DNAArtificial
sequenceForward Primer F6 for nef-N terminus 174gaccgttcgt
cagcgtatgg accgtaccga accggctgct 4017544DNAArtificial
sequenceForward Primer F7 for nef-N terminus 175cccgaattcc
atatggtcga cccgaccgtt cgtcagcgta tgga 4417642DNAArtificial
sequenceReverser Primer R1 for nef-N terminus 176acgcagcgga
acctgcggac gaaccggaaa accaacttct tc 4217739DNAArtificial
sequenceReverser Primer R2 for nef-N terminus 177aacagcagct
ttgtaggtca tcggacgcag cggaacccg 3917843DNAArtificial
sequenceReverser Primer R3 for nef-N terminus 178ctttttcttt
caggaagtgg ctgatgtcaa cagcagcttt gta 4317939DNAArtificial
sequenceReverser Primer R4 for nef-N terminus 179gtagatcaga
ccttccaggc cgcctttttc tttcaggaa 3918041DNAArtificial
sequenceReverser Primer R5 for nef-N terminus 180tccaggattt
cctgacgttt ctggctgtag atcagacctt c 4118140DNAArtificial
sequenceReverser Primer R6 for nef-N terminus 181agccctgggt
gtggtagatc cacaggtcca ggatttcctg 4018239DNAArtificial
sequenceReverser Primer R7 for nef-N terminus 182gtagttctgc
cagtccggga agtagccctg ggtgtggta 3918344DNAArtificial
sequenceReverser Primer R8 for nef-N terminus 183agcgggtaac
ggatacccgg acccggggtg tagttctgcc agtc 4418466DNAArtificial
sequenceReverser Primer 9 for nef-N terminus 184aaaaaaattc
ccattatttt tctcgagcag tttgaaacac cagccgaagg tcagcgggta 60acggat
6618539DNAArtificial sequenceForward Primer F1 for nef-C terminus
185ctgcacccga tcagccagca cggtatggac gacccggaa 3918639DNAArtificial
sequenceForward Primer F2 for nef-C terminus 186aacgaaggtg
ataacaactg cctgctgcac ccgatcagc 3918740DNAArtificial
sequenceForward Primer F3 for nef-C terminus 187tgacccggaa
caggttgaaa aagctaacga aggtgataac 4018854DNAArtificial
sequenceForward Primer F4 for nef-C terminus 188cccgaattcc
atatggtcga cttcctgaaa gttccggttg acccggaaca ggtt
5418939DNAArtificial sequenceReverse Primer R1 for nef-C terminus
189gaatttccac atcagaactt ctttttccgg gtcgtccat 3919040DNAArtificial
sequenceReverse Primer R2 for nef-C terminus 190tgtgctggaa
agccagacgg ctgtcgaatt tccacatcag 4019139DNAArtificial
sequenceReverse Primer R3 for nef-C terminus 191ttccgggtgt
ttttcacgag cgatgtgctg gaaagccag 3919263DNAArtificial
sequenceReverse Primer R4 for nef-C terminus 192aaaaaaattc
ccattatttt tctcgagacc caggcagtct ttgtagtatt ccgggtgttt 60ttc
6319340DNAArtificial sequenceForward Primer F1 for tat
193ctttatctct aaaggtctgg gtatcagcta cggtcgtaaa 4019446DNAArtificial
sequenceForward Primer 2 for tat 194caaaaaatgt tgtttccact
gcccggtttg ctttatctct aaaggt 4619539DNAArtificial sequenceForward
Primer F3 for tat 195accgcttgca acaactgcta ctgcaaaaaa tgttgtttc
3919639DNAArtificial sequenceForward Primer F4 for tat
196aaacacccgg gtagccagcc gcgtaccgct tgcaacaac 3919743DNAArtificial
sequenceForward Primer F5 for tat 197accggttgac ccgcgtctgg
aaccgtggaa acacccgggt agc 4319839DNAArtificial sequenceForward
Primer F6 for tat 198gaactgaaag gtatcggtat ggaaccggtt gacccgcgt
3919945DNAArtificial sequenceForward Primer F7 for tat
199cccgaattcc atatggtcga ccgtgacgaa ctgaaaggta tcggt
4520039DNAArtificial sequenceReverse Primer R1 for tat
200gagcgcggcg gcgctggcga cgctttttac gccgtagct 3920142DNAArtificial
sequenceReverse Primer R2 for tat 201aacctggtgg gtttcggagt
cctgcggagc gcggcggcgc tg 4220241DNAArtificial sequenceReverse
Primer R3 for tat 202tggctggtcg gttgcttgct caggctaacc tggtgggttt c
4120340DNAArtificial sequenceReverse Primer R4 for tat
203gaccggtcgg gtcaccacgc agctggctgg tcggttgctt 4020439DNAArtificial
sequenceReverse Primer 5 for tat 204aacttttttt ttgctttctt
tcggaccggt cgggtcacc 3920539DNAArtificial sequenceReverse Primer R6
for tat 205cgggtcggtt tcggtttcac gttcaacttt ttttttgct
3920651DNAArtificial sequenceReverse Primer R7 for tat
206aaaaaaattc tcattatttt tctcgagaac gttcgggtcg gtttcggttt c
5120740DNAArtificial sequenceForward Primer F1 for rev
207tcagatccac tccatcagcg aacgtatcct gatcacctgc 4020848DNAArtificial
sequenceForward Primer F2 for rev 208aaccgccgcc gccgctggcg
tgctcgtcag cgtcagatcc actccatc 4820945DNAArtificial sequenceForward
Primer F3 for rev 209aaaccggaag gttaccgtcg tgtccgtcgt aaccgccgcc
gccgc 4521042DNAArtificial sequenceForward Primer F4 for rev
210accctgtacc agtctaaccc gtacccgaaa ccggaaggtt ac
4221139DNAArtificial sequenceForward Primer F5 for rev
211ctgctggctg ttcgtatcat caaaaccctg taccagtct 3921248DNAArtificial
sequenceForward Primer F6 for rev 212ccccccgaat tccatatggt
cgacctgctg gctgttcgta tcatcaaa 4821340DNAArtificial sequenceReverse
Primer R1 for rev 213gaaccggttc ggtcggacga cccaggcagg tgatcaggat
4021439DNAArtificial sequenceReverse Primer R2 for rev
214ttcgatcggc ggcagttgca gcggaaccgg ttcggtcgg 3921539DNAArtificial
sequenceReverse Primer R3 for rev 215ttcgctacag ttgatgttca
gacgttcgat cggcggcag 3921639DNAArtificial sequenceReverse Primer R4
for rev 216ctgggtaccg ctggtaccac cgctttcgct acagttgat
3921759DNAArtificial sequenceReverse Primer R5 for rev
217aaaaaaattc ccattatttt tctcgagcgg gttaccaaca cgctgggtac cgctggtac
5921816PRTArtificial sequenceFusion protein gp120-41-N1 218Val Glu
Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys1 5 10
1521916PRTArtificial sequenceFusion protein gp120-41-N2 219Lys Val
Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Cys Lys1 5 10
1522015PRTArtificial sequenceFusion protein gp120-41-N3 220Ala Pro
Thr Lys Cys Lys Arg Arg Val Val Gln Arg Glu Lys Arg1 5 10
1522114PRTArtificial sequenceFusion protein gp120-41-C1 221Gln Ala
Arg Val Trp Arg Tyr Leu Lys Asp Gln Gln Leu Leu1 5
1022217PRTArtificial sequenceFusion protein gp120-41-C2 222Gly Ile
Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Thr Ala Val1 5 10
15Pro22315PRTArtificial sequenceFusion protein gp120-41-C3 223Ala
Val Pro Trp Asn Ala Ser Ser Trp Ser Asn Lys Leu Asp Arg1 5 10
1522420PRTHuman immunodeficiency virus 224Pro Val Asp Pro Arg Leu
Glu Pro Trp Lys His Pro Gly Ser Gln Pro1 5 10 15Arg Thr Ala
Cys2022520PRTHuman immunodeficiency virus 225Gln Leu Arg Gly Asp
Pro Thr Gly Pro Lys Glu Ser Lys Lys Lys Val1 5 10 15Glu Arg Glu
Thr2022620PRTHuman immunodeficiency virus 226Ser Tyr Gly Arg Lys
Lys Arg Arg Gln Arg Arg Arg Ala Pro Gln Asp1 5 10 15Ser Glu Thr
His2022720PRTHuman immunodeficiency virus 227Gln Ser Asn Pro Tyr
Pro Lys Pro Glu Gly Tyr Arg Arg Val Arg Arg1 5 10 15Asn Arg Arg
Arg2022820PRTHuman immunodeficiency virus 228Asn Cys Ser Glu Ser
Gly Gly Thr Ser Gly Thr Gln Arg Val Gly Asn1 5 10 15Pro Leu Glu
Lys2022918PRTHuman immunodeficiency virus 229Ser Lys Leu Lys Lys
Gly Trp Pro Thr Val Arg Gln Arg Met Asp Arg1 5 10 15Thr
Glu23018PRTHuman immunodeficiency virus 230Thr Gln Gly Tyr Phe Pro
Asp Trp Gln Asn Tyr Thr Pro Gly Pro Gly1 5 10 15Ile
Arg23115PRTHuman immunodeficiency virus 231Val Asp Pro Glu Gln Val
Glu Lys Ala Asn Glu Gly Asp Asn Asn1 5 10 1523215PRTHuman
immunodeficiency virus 232Ile Ser Gln His Gly Met Asp Asp Pro Glu
Lys Glu Val Leu Met1 5 10 1523320PRTHuman immunodeficiency virus
233Gln His Ile Ala Arg Glu Lys His Pro Glu Tyr Tyr Lys Asp Cys Leu1
5 10 15Gly Leu Glu Lys2023418PRTHuman immunodeficiency virus 234Pro
Glu Phe His Met Val Asp Arg Asp Glu Leu Lys Gly Ile Gly Met1 5 10
15Thr Asn23515PRTHuman immunodeficiency virus 235Arg Met Tyr Ser
Pro Thr Met Thr Asn Asn Pro Pro Ile Pro Val1 5 10
152361563DNAArtificial sequenceFusion gene PE(delta
III)-HIV-Gag24-K3 236atgggcagca gccatcatca tcatcatcac agcagcggcc
tggtgccgcg cggcagccat 60atggccgaag aagctttcga cctctggaac
gaatgcgcca
aagcctgcgt gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc
gtcgacccgg ccatcgccga caccaacggc 180cagggcgtgc tgcactactc
catggtcctg gagggcggca acgacgcgct caagctggcc 240atcgacaacg
ccctcagcat caccagcgac ggcctgacca tccgcctcga aggcggcgtc
300gagccgaaca agccggtgcg ctacagctac acgcgccagg cgcgcggcag
ttggtcgctg 360aactggctgg taccgatcgg ccacgagaag ccctcgaaca
tcaaggtgtt catccacgaa 420ctgaacgccg gcaaccagct cagccacatg
tcgccgatct acaccatcga gatgggcgac 480gagttgctgg cgaagctggc
gcgcgatgcc accttcttcg tcagggcgca cgagagcaac 540gagatgcagc
cgacgctcgc catcagccat gccggggtca gcgtggtcat ggcccagacc
600cagccgcgcc gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt
gtgcctgctc 660gacccgctgg acggggtcta caactacctc gcccagcaac
gctgcaacct cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc
ggcaacccgg cgaagcatga cctggacatc 780aaacccacgg tcatcagtca
tcgcctgcac tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc
aggcttgcca cctgccgctg gagactttca cccgtcatcg ccagccgcgc
900ggctgggaac aactggagca gtgcggctat ccggtgcagc ggctggtcgc
cctctacctg 960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc
gcaacgccct ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc
cgcgagcagc cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga
gagcgagcgc ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg
ccaacgccga cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc
1200gcgggcccgg cggacagcgg cgacgccctg ctggagcgca actatcccac
tggcgcggag 1260ttcctcggcg acggcggcga cgtcgaattc catatggtcg
accgtgacga actgaaaggt 1320atcggtatga ccaacaaccc gccgatcccg
gttggtgaaa tctacaaacg ttggatcatc 1380ctgggtctga acaaaatcgt
tcgtatgtac agcccgacca tgaccaacaa cccgccaatt 1440ccagtaggcg
agatttaccg ttggatcatc ctgggtctga acaaaatcgt tcgcatgtac
1500agcccgactc tcgagtacct caaaaaagac gaactgcgtg tagaactgaa
agacgaactg 1560taa 1563237520PRTArtificial sequenceFusion protein
PE(delta III)-HIV Gag24-K3 237Met Gly Ser Ser His His His His His
His Ser Ser Gly Leu Val Pro1 5 10 15Arg Gly Ser His Met Ala Glu Glu
Ala Phe Asp Leu Trp Asn Glu Cys20 25 30Ala Lys Ala Cys Val Leu Asp
Leu Lys Asp Gly Val Arg Ser Ser Arg35 40 45Met Ser Val Asp Pro Ala
Ile Ala Asp Thr Asn Gly Gln Gly Val Leu50 55 60His Tyr Ser Met Val
Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala65 70 75 80Ile Asp Asn
Ala Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu85 90 95Glu Gly
Gly Val Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg100 105
110Gln Ala Arg Gly Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly
His115 120 125Glu Lys Pro Ser Asn Ile Lys Val Phe Ile His Glu Leu
Asn Ala Gly130 135 140Asn Gln Leu Ser His Met Ser Pro Ile Tyr Thr
Ile Glu Met Gly Asp145 150 155 160Glu Leu Leu Ala Lys Leu Ala Arg
Asp Ala Thr Phe Phe Val Arg Ala165 170 175His Glu Ser Asn Glu Met
Gln Pro Thr Leu Ala Ile Ser His Ala Gly180 185 190Val Ser Val Val
Met Ala Gln Thr Gln Pro Arg Arg Glu Lys Arg Trp195 200 205Ser Glu
Trp Ala Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp210 215
220Gly Val Tyr Asn Tyr Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp
Thr225 230 235 240Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn
Pro Ala Lys His245 250 255Asp Leu Asp Ile Lys Pro Thr Val Ile Ser
His Arg Leu His Phe Pro260 265 270Glu Gly Gly Ser Leu Ala Ala Leu
Thr Ala His Gln Ala Cys His Leu275 280 285Pro Leu Glu Thr Phe Thr
Arg His Arg Gln Pro Arg Gly Trp Glu Gln290 295 300Leu Glu Gln Cys
Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu305 310 315 320Ala
Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala325 330
335Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg
Glu340 345 350Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala
Ala Glu Ser355 360 365Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp
Glu Ala Gly Ala Ala370 375 380Asn Ala Asp Val Val Ser Leu Thr Cys
Pro Val Ala Ala Gly Glu Cys385 390 395 400Ala Gly Pro Ala Asp Ser
Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro405 410 415Thr Gly Ala Glu
Phe Leu Gly Asp Gly Gly Asp Val Glu Phe His Met420 425 430Val Asp
Arg Asp Glu Leu Lys Gly Ile Gly Met Thr Asn Asn Pro Pro435 440
445Ile Pro Val Gly Glu Ile Tyr Lys Arg Trp Ile Ile Leu Gly Leu
Asn450 455 460Lys Ile Val Arg Met Tyr Ser Pro Thr Met Thr Asn Asn
Pro Pro Ile465 470 475 480Pro Val Gly Glu Ile Tyr Arg Trp Ile Ile
Leu Gly Leu Asn Lys Ile485 490 495Val Arg Met Tyr Ser Pro Thr Leu
Glu Tyr Leu Lys Lys Asp Glu Leu500 505 510Arg Val Glu Leu Lys Asp
Glu Leu515 5202381863DNAArtificial sequenceFusion gene PE(delta
III)-HIV Gag24-gp120-41-K3 238atgggcagca gccatcatca tcatcatcac
agcagcggcc tggtgccgcg cggcagccat 60atggccgaag aagctttcga cctctggaac
gaatgcgcca aagcctgcgt gctcgacctc 120aaggacggcg tgcgttccag
ccgcatgagc gtcgacccgg ccatcgccga caccaacggc 180cagggcgtgc
tgcactactc catggtcctg gagggcggca acgacgcgct caagctggcc
240atcgacaacg ccctcagcat caccagcgac ggcctgacca tccgcctcga
aggcggcgtc 300gagccgaaca agccggtgcg ctacagctac acgcgccagg
cgcgcggcag ttggtcgctg 360aactggctgg taccgatcgg ccacgagaag
ccctcgaaca tcaaggtgtt catccacgaa 420ctgaacgccg gcaaccagct
cagccacatg tcgccgatct acaccatcga gatgggcgac 480gagttgctgg
cgaagctggc gcgcgatgcc accttcttcg tcagggcgca cgagagcaac
540gagatgcagc cgacgctcgc catcagccat gccggggtca gcgtggtcat
ggcccagacc 600cagccgcgcc gggaaaagcg ctggagcgaa tgggccagcg
gcaaggtgtt gtgcctgctc 660gacccgctgg acggggtcta caactacctc
gcccagcaac gctgcaacct cgacgatacc 720tgggaaggca agatctaccg
ggtgctcgcc ggcaacccgg cgaagcatga cctggacatc 780aaacccacgg
tcatcagtca tcgcctgcac tttcccgagg gcggcagcct ggccgcgctg
840accgcgcacc aggcttgcca cctgccgctg gagactttca cccgtcatcg
ccagccgcgc 900ggctgggaac aactggagca gtgcggctat ccggtgcagc
ggctggtcgc cctctacctg 960gcggcgcggc tgtcgtggaa ccaggtcgac
caggtgatcc gcaacgccct ggccagcccc 1020ggcagcggcg gcgacctggg
cgaagcgatc cgcgagcagc cggagcaggc ccgtctggcc 1080ctgaccctgg
ccgccgccga gagcgagcgc ttcgtccggc agggcaccgg caacgacgag
1140gccggcgcgg ccaacgccga cgtggtgagc ctgacctgcc cggtcgccgc
cggtgaatgc 1200gcgggcccgg cggacagcgg cgacgccctg ctggagcgca
actatcccac tggcgcggag 1260ttcctcggcg acggcggcga cgtcgaattc
catatggtcg accgtgacga actgaaaggt 1320atcggtatga ccaacaaccc
gccgatcccg gttggtgaaa tctacaaacg ttggatcatc 1380ctgggtctga
acaaaatcgt tcgtatgtac agcccgacca tgaccaacaa cccgccaatt
1440ccagtaggcg agatttaccg ttggatcatc ctgggtctga acaaaatcgt
tcgcatgtac 1500agcccgactc tcgacgttga aaaactgtgg gttaccgttt
actacggtgt tccggtttgg 1560aaaaaagttg ttaaaatcga accgctgggt
gttgctccga ccaaatgcaa acgtcgtgtt 1620gttcagcgtg aaaagcgtgg
tggcggtggc ggtcaagctc gtgttatcgc tgttgaacgt 1680tacctgaaag
accagcagct gctgggtatc tggggtggta gcggtaaact gatctgctgc
1740accaccgctg ttccgtggaa cagcagctgg agcaacaaac tggaccgtat
ctggaacaac 1800atgacctggc tcgagtacct caaaaaagac gaactgcgtg
tagaactgaa agacgaactg 1860taa 1863239620PRTArtificial
sequenceFusion protein PE(delta III)-HIV Gag24-gp120-41-K3 239Met
Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro1 5 10
15Arg Gly Ser His Met Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys20
25 30Ala Lys Ala Cys Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser
Arg35 40 45Met Ser Val Asp Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly
Val Leu50 55 60His Tyr Ser Met Val Leu Glu Gly Gly Asn Asp Ala Leu
Lys Leu Ala65 70 75 80Ile Asp Asn Ala Leu Ser Ile Thr Ser Asp Gly
Leu Thr Ile Arg Leu85 90 95Glu Gly Gly Val Glu Pro Asn Lys Pro Val
Arg Tyr Ser Tyr Thr Arg100 105 110Gln Ala Arg Gly Ser Trp Ser Leu
Asn Trp Leu Val Pro Ile Gly His115 120 125Glu Lys Pro Ser Asn Ile
Lys Val Phe Ile His Glu Leu Asn Ala Gly130 135 140Asn Gln Leu Ser
His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp145 150 155 160Glu
Leu Leu Ala Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala165 170
175His Glu Ser Asn Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala
Gly180 185 190Val Ser Val Val Met Ala Gln Thr Gln Pro Arg Arg Glu
Lys Arg Trp195 200 205Ser Glu Trp Ala Ser Gly Lys Val Leu Cys Leu
Leu Asp Pro Leu Asp210 215 220Gly Val Tyr Asn Tyr Leu Ala Gln Gln
Arg Cys Asn Leu Asp Asp Thr225 230 235 240Trp Glu Gly Lys Ile Tyr
Arg Val Leu Ala Gly Asn Pro Ala Lys His245 250 255Asp Leu Asp Ile
Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro260 265 270Glu Gly
Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu275 280
285Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu
Gln290 295 300Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala
Leu Tyr Leu305 310 315 320Ala Ala Arg Leu Ser Trp Asn Gln Val Asp
Gln Val Ile Arg Asn Ala325 330 335Leu Ala Ser Pro Gly Ser Gly Gly
Asp Leu Gly Glu Ala Ile Arg Glu340 345 350Gln Pro Glu Gln Ala Arg
Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser355 360 365Glu Arg Phe Val
Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala370 375 380Asn Ala
Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys385 390 395
400Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr
Pro405 410 415Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Glu
Phe His Met420 425 430Val Asp Arg Asp Glu Leu Lys Gly Ile Gly Met
Thr Asn Asn Pro Pro435 440 445Ile Pro Val Gly Glu Ile Tyr Lys Arg
Trp Ile Ile Leu Gly Leu Asn450 455 460Lys Ile Val Arg Met Tyr Ser
Pro Thr Met Thr Asn Asn Pro Pro Ile465 470 475 480Pro Val Gly Glu
Ile Tyr Arg Trp Ile Ile Leu Gly Leu Asn Lys Ile485 490 495Val Arg
Met Tyr Ser Pro Thr Leu Asp Val Glu Lys Leu Trp Val Thr500 505
510Val Tyr Tyr Gly Val Pro Val Trp Lys Lys Val Val Lys Ile Glu
Pro515 520 525Leu Gly Val Ala Pro Thr Lys Cys Lys Arg Arg Val Val
Gln Arg Glu530 535 540Lys Arg Gly Gly Gly Gly Gly Gln Ala Arg Val
Ile Ala Val Glu Arg545 550 555 560Tyr Leu Lys Asp Gln Gln Leu Leu
Gly Ile Trp Gly Gly Ser Gly Lys565 570 575Leu Ile Cys Cys Thr Thr
Ala Val Pro Trp Asn Ser Ser Trp Ser Asn580 585 590Lys Leu Asp Arg
Ile Trp Asn Asn Met Thr Trp Leu Glu Tyr Leu Lys595 600 605Lys Asp
Glu Leu Arg Val Glu Leu Lys Asp Glu Leu610 615
6202401749DNAArtificial sequenceFusion gene PE(delta III)-HIV
nef-N-K3 240atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
cggcagccat 60atggccgaag aagctttcga cctctggaac gaatgcgcca aagcctgcgt
gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc gtcgacccgg
ccatcgccga caccaacggc 180cagggcgtgc tgcactactc catggtcctg
gagggcggca acgacgcgct caagctggcc 240atcgacaacg ccctcagcat
caccagcgac ggcctgacca tccgcctcga aggcggcgtc 300gagccgaaca
agccggtgcg ctacagctac acgcgccagg cgcgcggcag ttggtcgctg
360aactggctgg taccgatcgg ccacgagaag ccctcgaaca tcaaggtgtt
catccacgaa 420ctgaacgccg gcaaccagct cagccacatg tcgccgatct
acaccatcga gatgggcgac 480gagttgctgg cgaagctggc gcgcgatgcc
accttcttcg tcagggcgca cgagagcaac 540gagatgcagc cgacgctcgc
catcagccat gccggggtca gcgtggtcat ggcccagacc 600cagccgcgcc
gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt gtgcctgctc
660gacccgctgg acggggtcta caactacctc gcccagcaac gctgcaacct
cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc ggcaacccgg
cgaagcatga cctggacatc 780aaacccacgg tcatcagtca tcgcctgcac
tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc aggcttgcca
cctgccgctg gagactttca cccgtcatcg ccagccgcgc 900ggctgggaac
aactggagca gtgcggctat ccggtgcagc ggctggtcgc cctctacctg
960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc gcaacgccct
ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc cgcgagcagc
cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga gagcgagcgc
ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg ccaacgccga
cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc 1200gcgggcccgg
cggacagcgg cgacgccctg ctggagcgca actatcccac tggcgcggag
1260ttcctcggcg acggcggcga cgtcgaattc catatggtcg acccgaccgt
tcgtcagcgt 1320atggaccgta ccgaaccggc tgctgagggt gttggtgctg
ttagccgtga cctggaaaaa 1380cacggtgcta tcacctcttc taacaccgct
gctaccaacg ctgactgcgc ttggctggaa 1440gctcaggaag aagaagaagt
tggttttccg gttcgtccgc aggttccgct gcgtccgatg 1500acctacaaag
ctgctgttga catcagccac ttcctgaaag aaaaaggcgg cctggaaggt
1560ctgatctaca gccagaaacg tcaggaaatc ctggacctgt ggatctacca
cacccagggc 1620tacttcccgg actggcagaa ctacaccccg ggtccgggta
tccgttaccc gctgaccttc 1680ggctggtgtt tcaaactgct cgagtacctc
aaaaaagacg aactgcgtgt agaactgaaa 1740gacgaactg
1749241583PRTArtificial sequencefusion protein PE(delta III)-HIV
Nef-N-K3 241Met Gly Ser Ser His His His His His His Ser Ser Gly Leu
Val Pro1 5 10 15Arg Gly Ser His Met Ala Glu Glu Ala Phe Asp Leu Trp
Asn Glu Cys20 25 30Ala Lys Ala Cys Val Leu Asp Leu Lys Asp Gly Val
Arg Ser Ser Arg35 40 45Met Ser Val Asp Pro Ala Ile Ala Asp Thr Asn
Gly Gln Gly Val Leu50 55 60His Tyr Ser Met Val Leu Glu Gly Gly Asn
Asp Ala Leu Lys Leu Ala65 70 75 80Ile Asp Asn Ala Leu Ser Ile Thr
Ser Asp Gly Leu Thr Ile Arg Leu85 90 95Glu Gly Gly Val Glu Pro Asn
Lys Pro Val Arg Tyr Ser Tyr Thr Arg100 105 110Gln Ala Arg Gly Ser
Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His115 120 125Glu Lys Pro
Ser Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly130 135 140Asn
Gln Leu Ser His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp145 150
155 160Glu Leu Leu Ala Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg
Ala165 170 175His Glu Ser Asn Glu Met Gln Pro Thr Leu Ala Ile Ser
His Ala Gly180 185 190Val Ser Val Val Met Ala Gln Thr Gln Pro Arg
Arg Glu Lys Arg Trp195 200 205Ser Glu Trp Ala Ser Gly Lys Val Leu
Cys Leu Leu Asp Pro Leu Asp210 215 220Gly Val Tyr Asn Tyr Leu Ala
Gln Gln Arg Cys Asn Leu Asp Asp Thr225 230 235 240Trp Glu Gly Lys
Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His245 250 255Asp Leu
Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro260 265
270Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His
Leu275 280 285Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly
Trp Glu Gln290 295 300Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu
Val Ala Leu Tyr Leu305 310 315 320Ala Ala Arg Leu Ser Trp Asn Gln
Val Asp Gln Val Ile Arg Asn Ala325 330 335Leu Ala Ser Pro Gly Ser
Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu340 345 350Gln Pro Glu Gln
Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser355 360 365Glu Arg
Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala370 375
380Asn Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu
Cys385 390 395 400Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu
Arg Asn Tyr Pro405 410 415Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly
Asp Val Glu Phe His Met420 425 430Val Asp Pro Thr Val Arg Gln Arg
Met Asp Arg Thr Glu Pro Ala Ala435 440 445Glu Gly Val Gly Ala Val
Ser Arg Asp Leu Glu Lys His Gly Ala Ile450 455 460Thr Ser Ser Asn
Thr Ala Ala Thr Asn Ala Asp Cys Ala Trp Leu Glu465 470 475 480Ala
Gln Glu Glu Glu Glu Val Gly Phe Pro Val Arg Pro Gln Val Pro485 490
495Leu Arg Pro Met Thr Tyr Lys Ala Ala Val Asp Ile Ser His Phe
Leu500 505 510Lys Glu Lys Gly Gly Leu Glu Gly Leu Ile Tyr Ser Gln
Lys Arg Gln515 520 525Glu Ile Leu Asp Leu Trp Ile Tyr His Thr Gln
Gly Tyr Phe Pro Asp530
535 540Trp Gln Asn Tyr Thr Pro Gly Pro Gly Ile Arg Tyr Pro Leu Thr
Phe545 550 555 560Gly Trp Cys Phe Lys Leu Leu Glu Tyr Leu Lys Lys
Asp Glu Leu Arg565 570 575Val Glu Leu Lys Asp Glu
Leu5802421881DNAArtificial sequenceFusion gene PE(delta III)-HIV
nef-C-K3 242atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
cggcagccat 60atggccgaag aagctttcga cctctggaac gaatgcgcca aagcctgcgt
gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc gtcgacccgg
ccatcgccga caccaacggc 180cagggcgtgc tgcactactc catggtcctg
gagggcggca acgacgcgct caagctggcc 240atcgacaacg ccctcagcat
caccagcgac ggcctgacca tccgcctcga aggcggcgtc 300gagccgaaca
agccggtgcg ctacagctac acgcgccagg cgcgcggcag ttggtcgctg
360aactggctgg taccgatcgg ccacgagaag ccctcgaaca tcaaggtgtt
catccacgaa 420ctgaacgccg gcaaccagct cagccacatg tcgccgatct
acaccatcga gatgggcgac 480gagttgctgg cgaagctggc gcgcgatgcc
accttcttcg tcagggcgca cgagagcaac 540gagatgcagc cgacgctcgc
catcagccat gccggggtca gcgtggtcat ggcccagacc 600cagccgcgcc
gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt gtgcctgctc
660gacccgctgg acggggtcta caactacctc gcccagcaac gctgcaacct
cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc ggcaacccgg
cgaagcatga cctggacatc 780aaacccacgg tcatcagtca tcgcctgcac
tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc aggcttgcca
cctgccgctg gagactttca cccgtcatcg ccagccgcgc 900ggctgggaac
aactggagca gtgcggctat ccggtgcagc ggctggtcgc cctctacctg
960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc gcaacgccct
ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc cgcgagcagc
cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga gagcgagcgc
ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg ccaacgccga
cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc 1200gcgggcccgg
cggacagcgg cgacgccctg ctggagcgca actatcccac tggcgcggag
1260ttcctcggcg acggcggcga cgtccgtcac cactttaccc cgagtgagcg
tcaattgtgt 1320ttgtcgtcaa tccagactgc ctttaatcaa ggcgctggta
cttgcatcct gtcagattct 1380gggcgtatca gttacactgt ggagtttagt
ttgcctacgc atcatactgt gcgcctgatc 1440cgcgttacag caccaccgtc
agcactcgac gcgaccgtct acaacggtag cagtaagtac 1500ggtgacacca
gcactagcaa cgtgcgtggt gaccttcaag tgttagctca gaaggcagaa
1560cgtactctgc ctacctcctt caacttcggt gccatcaagg caactcgtgt
tactgaattc 1620catatggtcg acttcctgaa agttccggtt gacccggaac
aggttgaaaa agctaacgaa 1680ggtgataaca actgcctgct gcacccgatc
agccagcacg gtatggacga cccggaaaaa 1740gaagttctga tgtggaaatt
cgacagccgt ctggctttcc agcacatcgc tcgtgaaaaa 1800cacccggaat
actacaaaga ctgcctgggt ctcgagtacc tcaaaaaaga cgaactgcgt
1860gtagaactga aagacgaact g 1881243627PRTArtificial sequenceFusion
protein PE(delta III)-HIV Nef-C-K3 243Met Gly Ser Ser His His His
His His His Ser Ser Gly Leu Val Pro1 5 10 15Arg Gly Ser His Met Ala
Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys20 25 30Ala Lys Ala Cys Val
Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg35 40 45Met Ser Val Asp
Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu50 55 60His Tyr Ser
Met Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala65 70 75 80Ile
Asp Asn Ala Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu85 90
95Glu Gly Gly Val Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr
Arg100 105 110Gln Ala Arg Gly Ser Trp Ser Leu Asn Trp Leu Val Pro
Ile Gly His115 120 125Glu Lys Pro Ser Asn Ile Lys Val Phe Ile His
Glu Leu Asn Ala Gly130 135 140Asn Gln Leu Ser His Met Ser Pro Ile
Tyr Thr Ile Glu Met Gly Asp145 150 155 160Glu Leu Leu Ala Lys Leu
Ala Arg Asp Ala Thr Phe Phe Val Arg Ala165 170 175His Glu Ser Asn
Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly180 185 190Val Ser
Val Val Met Ala Gln Thr Gln Pro Arg Arg Glu Lys Arg Trp195 200
205Ser Glu Trp Ala Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu
Asp210 215 220Gly Val Tyr Asn Tyr Leu Ala Gln Gln Arg Cys Asn Leu
Asp Asp Thr225 230 235 240Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala
Gly Asn Pro Ala Lys His245 250 255Asp Leu Asp Ile Lys Pro Thr Val
Ile Ser His Arg Leu His Phe Pro260 265 270Glu Gly Gly Ser Leu Ala
Ala Leu Thr Ala His Gln Ala Cys His Leu275 280 285Pro Leu Glu Thr
Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln290 295 300Leu Glu
Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu305 310 315
320Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn
Ala325 330 335Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala
Ile Arg Glu340 345 350Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu
Ala Ala Ala Glu Ser355 360 365Glu Arg Phe Val Arg Gln Gly Thr Gly
Asn Asp Glu Ala Gly Ala Ala370 375 380Asn Ala Asp Val Val Ser Leu
Thr Cys Pro Val Ala Ala Gly Glu Cys385 390 395 400Ala Gly Pro Ala
Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro405 410 415Thr Gly
Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Arg His His Phe420 425
430Thr Pro Ser Glu Arg Gln Leu Cys Leu Ser Ser Ile Gln Thr Ala
Phe435 440 445Asn Gln Gly Ala Gly Thr Cys Ile Leu Ser Asp Ser Gly
Arg Ile Ser450 455 460Tyr Thr Val Glu Phe Ser Leu Pro Thr His His
Thr Val Arg Leu Ile465 470 475 480Arg Val Thr Ala Pro Pro Ser Ala
Leu Asp Ala Thr Val Tyr Asn Gly485 490 495Ser Ser Lys Tyr Gly Asp
Thr Ser Thr Ser Asn Val Arg Gly Asp Leu500 505 510Gln Val Leu Ala
Gln Lys Ala Glu Arg Thr Leu Pro Thr Ser Phe Asn515 520 525Phe Gly
Ala Ile Lys Ala Thr Arg Val Thr Glu Phe His Met Val Asp530 535
540Phe Leu Lys Val Pro Val Asp Pro Glu Gln Val Glu Lys Ala Asn
Glu545 550 555 560Gly Asp Asn Asn Cys Leu Leu His Pro Ile Ser Gln
His Gly Met Asp565 570 575Asp Pro Glu Lys Glu Val Leu Met Trp Lys
Phe Asp Ser Arg Leu Ala580 585 590Phe Gln His Ile Ala Arg Glu Lys
His Pro Glu Tyr Tyr Lys Asp Cys595 600 605Leu Gly Leu Glu Tyr Leu
Lys Lys Asp Glu Leu Arg Val Glu Leu Lys610 615 620Asp Glu
Leu6252441953DNAArtificial sequenceFusion gene PE(delta III)-HIV
nef-NC-K3 244atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
cggcagccat 60atggccgaag aagctttcga cctctggaac gaatgcgcca aagcctgcgt
gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc gtcgacccgg
ccatcgccga caccaacggc 180cagggcgtgc tgcactactc catggtcctg
gagggcggca acgacgcgct caagctggcc 240atcgacaacg ccctcagcat
caccagcgac ggcctgacca tccgcctcga aggcggcgtc 300gagccgaaca
agccggtgcg ctacagctac acgcgccagg cgcgcggcag ttggtcgctg
360aactggctgg taccgatcgg ccacgagaag ccctcgaaca tcaaggtgtt
catccacgaa 420ctgaacgccg gcaaccagct cagccacatg tcgccgatct
acaccatcga gatgggcgac 480gagttgctgg cgaagctggc gcgcgatgcc
accttcttcg tcagggcgca cgagagcaac 540gagatgcagc cgacgctcgc
catcagccat gccggggtca gcgtggtcat ggcccagacc 600cagccgcgcc
gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt gtgcctgctc
660gacccgctgg acggggtcta caactacctc gcccagcaac gctgcaacct
cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc ggcaacccgg
cgaagcatga cctggacatc 780aaacccacgg tcatcagtca tcgcctgcac
tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc aggcttgcca
cctgccgctg gagactttca cccgtcatcg ccagccgcgc 900ggctgggaac
aactggagca gtgcggctat ccggtgcagc ggctggtcgc cctctacctg
960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc gcaacgccct
ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc cgcgagcagc
cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga gagcgagcgc
ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg ccaacgccga
cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc 1200gcgggcccgg
cggacagcgg cgacgccctg ctggagcgca actatcccac tggcgcggag
1260ttcctcggcg acggcggcga cgtcgaattc catatggtcg acccgaccgt
tcgtcagcgt 1320atggaccgta ccgaaccggc tgctgagggt gttggtgctg
ttagccgtga cctggaaaaa 1380cacggtgcta tcacctcttc taacaccgct
gctaccaacg ctgactgcgc ttggctggaa 1440gctcaggaag aagaagaagt
tggttttccg gttcgtccgc aggttccgct gcgtccgatg 1500acctacaaag
ctgctgttga catcagccac ttcctgaaag aaaaaggcgg cctggaaggt
1560ctgatctaca gccagaaacg tcaggaaatc ctggacctgt ggatctacca
cacccagggc 1620tacttcccgg actggcagaa ctacaccccg ggtccgggta
tccgttaccc gctgaccttc 1680ggctggtgtt tcaaactgct cgacttcctg
aaagttccgg ttgacccgga acaggttgaa 1740aaagctaacg aaggtgataa
caactgcctg ctgcacccga tcagccagca cggtatggac 1800gacccggaaa
aagaagttct gatgtggaaa ttcgacagcc gtctggcttt ccagcacatc
1860gctcgtgaaa aacacccgga atactacaaa gactgcctgg gtctcgagta
cctcaaaaaa 1920gacgaactgc gtgtagaact gaaagacgaa ctg
1953245651PRTArtificial sequenceFusion protein PE(delta III)-HIV
Nef-NC-K3 245Met Gly Ser Ser His His His His His His Ser Ser Gly
Leu Val Pro1 5 10 15Arg Gly Ser His Met Ala Glu Glu Ala Phe Asp Leu
Trp Asn Glu Cys20 25 30Ala Lys Ala Cys Val Leu Asp Leu Lys Asp Gly
Val Arg Ser Ser Arg35 40 45Met Ser Val Asp Pro Ala Ile Ala Asp Thr
Asn Gly Gln Gly Val Leu50 55 60His Tyr Ser Met Val Leu Glu Gly Gly
Asn Asp Ala Leu Lys Leu Ala65 70 75 80Ile Asp Asn Ala Leu Ser Ile
Thr Ser Asp Gly Leu Thr Ile Arg Leu85 90 95Glu Gly Gly Val Glu Pro
Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg100 105 110Gln Ala Arg Gly
Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His115 120 125Glu Lys
Pro Ser Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly130 135
140Asn Gln Leu Ser His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly
Asp145 150 155 160Glu Leu Leu Ala Lys Leu Ala Arg Asp Ala Thr Phe
Phe Val Arg Ala165 170 175His Glu Ser Asn Glu Met Gln Pro Thr Leu
Ala Ile Ser His Ala Gly180 185 190Val Ser Val Val Met Ala Gln Thr
Gln Pro Arg Arg Glu Lys Arg Trp195 200 205Ser Glu Trp Ala Ser Gly
Lys Val Leu Cys Leu Leu Asp Pro Leu Asp210 215 220Gly Val Tyr Asn
Tyr Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr225 230 235 240Trp
Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His245 250
255Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe
Pro260 265 270Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala
Cys His Leu275 280 285Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro
Arg Gly Trp Glu Gln290 295 300Leu Glu Gln Cys Gly Tyr Pro Val Gln
Arg Leu Val Ala Leu Tyr Leu305 310 315 320Ala Ala Arg Leu Ser Trp
Asn Gln Val Asp Gln Val Ile Arg Asn Ala325 330 335Leu Ala Ser Pro
Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu340 345 350Gln Pro
Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser355 360
365Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala
Ala370 375 380Asn Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala
Gly Glu Cys385 390 395 400Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu
Leu Glu Arg Asn Tyr Pro405 410 415Thr Gly Ala Glu Phe Leu Gly Asp
Gly Gly Asp Val Glu Phe His Met420 425 430Val Asp Pro Thr Val Arg
Gln Arg Met Asp Arg Thr Glu Pro Ala Ala435 440 445Glu Gly Val Gly
Ala Val Ser Arg Asp Leu Glu Lys His Gly Ala Ile450 455 460Thr Ser
Ser Asn Thr Ala Ala Thr Asn Ala Asp Cys Ala Trp Leu Glu465 470 475
480Ala Gln Glu Glu Glu Glu Val Gly Phe Pro Val Arg Pro Gln Val
Pro485 490 495Leu Arg Pro Met Thr Tyr Lys Ala Ala Val Asp Ile Ser
His Phe Leu500 505 510Lys Glu Lys Gly Gly Leu Glu Gly Leu Ile Tyr
Ser Gln Lys Arg Gln515 520 525Glu Ile Leu Asp Leu Trp Ile Tyr His
Thr Gln Gly Tyr Phe Pro Asp530 535 540Trp Gln Asn Tyr Thr Pro Gly
Pro Gly Ile Arg Tyr Pro Leu Thr Phe545 550 555 560Gly Trp Cys Phe
Lys Leu Leu Asp Phe Leu Lys Val Pro Val Asp Pro565 570 575Glu Gln
Val Glu Lys Ala Asn Glu Gly Asp Asn Asn Cys Leu Leu His580 585
590Pro Ile Ser Gln His Gly Met Asp Asp Pro Glu Lys Glu Val Leu
Met595 600 605Trp Lys Phe Asp Ser Arg Leu Ala Phe Gln His Ile Ala
Arg Glu Lys610 615 620His Pro Glu Tyr Tyr Lys Asp Cys Leu Gly Leu
Glu Tyr Leu Lys Lys625 630 635 640Asp Glu Leu Arg Val Glu Leu Lys
Asp Glu Leu645 6502461947DNAArtificial sequenceFusion gene PE(delta
III)-HIV rev-K3 246atgggcagca gccatcatca tcatcatcac agcagcggcc
tggtgccgcg cggcagccat 60atggccgaag aagctttcga cctctggaac gaatgcgcca
aagcctgcgt gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc
gtcgacccgg ccatcgccga caccaacggc 180cagggcgtgc tgcactactc
catggtcctg gagggcggca acgacgcgct caagctggcc 240atcgacaacg
ccctcagcat caccagcgac ggcctgacca tccgcctcga aggcggcgtc
300gagccgaaca agccggtgcg ctacagctac acgcgccagg cgcgcggcag
ttggtcgctg 360aactggctgg taccgatcgg ccacgagaag ccctcgaaca
tcaaggtgtt catccacgaa 420ctgaacgccg gcaaccagct cagccacatg
tcgccgatct acaccatcga gatgggcgac 480gagttgctgg cgaagctggc
gcgcgatgcc accttcttcg tcagggcgca cgagagcaac 540gagatgcagc
cgacgctcgc catcagccat gccggggtca gcgtggtcat ggcccagacc
600cagccgcgcc gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt
gtgcctgctc 660gacccgctgg acggggtcta caactacctc gcccagcaac
gctgcaacct cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc
ggcaacccgg cgaagcatga cctggacatc 780aaacccacgg tcatcagtca
tcgcctgcac tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc
aggcttgcca cctgccgctg gagactttca cccgtcatcg ccagccgcgc
900ggctgggaac aactggagca gtgcggctat ccggtgcagc ggctggtcgc
cctctacctg 960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc
gcaacgccct ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc
cgcgagcagc cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga
gagcgagcgc ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg
ccaacgccga cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc
1200gcgggcccgg cggacagcgg cgacgccctg ctggagcgca actatcccac
tggcgcggag 1260ttcctcggcg acggcggcga cgtccgtcac cactttaccc
cgagtgagcg tcaattgtgt 1320ttgtcgtcaa tccagactgc ctttaatcaa
ggcgctggta cttgcatcct gtcagattct 1380gggcgtatca gttacactgt
ggagtttagt ttgcctacgc atcatactgt gcgcctgatc 1440cgcgttacag
caccaccgtc agcactcgac gcgaccgtct acaacggtag cagtaagtac
1500ggtgacacca gcactagcaa cgtgcgtggt gaccttcaag tgttagctca
gaaggcagaa 1560cgtactctgc ctacctcctt caacttcggt gccatcaagg
caactcgtgt tactgaattc 1620catatggtcg acctgctggc tgttcgtatc
atcaaaaccc tgtaccagtc taacccgtac 1680ccgaaaccgg aaggttaccg
tcgtgtccgt cgtaaccgcc gccgccgctg gcgtgctcgt 1740cagcgtcaga
tccactccat cagcgaacgt atcctgatca cctgcctggg tcgtccgacc
1800gaaccggttc cgctgcaact gccgccgatc gaacgtctga acatcaactg
tagcgaaagc 1860ggtggtacca gcggtaccca gcgtgttggt aacccgctcg
agtacctcaa aaaagacgaa 1920ctgcgtgtag aactgaaaga cgaactg
1947247649PRTArtificial sequenceFusion protein PE(delta III)-HIV
Rev-K3 247Met Gly Ser Ser His His His His His His Ser Ser Gly Leu
Val Pro1 5 10 15Arg Gly Ser His Met Ala Glu Glu Ala Phe Asp Leu Trp
Asn Glu Cys20 25 30Ala Lys Ala Cys Val Leu Asp Leu Lys Asp Gly Val
Arg Ser Ser Arg35 40 45Met Ser Val Asp Pro Ala Ile Ala Asp Thr Asn
Gly Gln Gly Val Leu50 55 60His Tyr Ser Met Val Leu Glu Gly Gly Asn
Asp Ala Leu Lys Leu Ala65 70 75 80Ile Asp Asn Ala Leu Ser Ile Thr
Ser Asp Gly Leu Thr Ile Arg Leu85 90 95Glu Gly Gly Val Glu Pro Asn
Lys Pro Val Arg Tyr Ser Tyr Thr Arg100 105 110Gln Ala Arg Gly Ser
Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His115 120 125Glu Lys Pro
Ser Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly130 135 140Asn
Gln Leu Ser His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp145 150
155 160Glu Leu Leu Ala Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg
Ala165 170 175His Glu Ser Asn Glu Met Gln Pro Thr Leu Ala Ile Ser
His Ala Gly180 185 190Val Ser Val Val Met Ala Gln Thr Gln Pro Arg
Arg Glu Lys Arg Trp195 200 205Ser Glu Trp Ala Ser Gly Lys Val Leu
Cys Leu Leu Asp Pro Leu Asp210 215 220Gly Val Tyr Asn Tyr Leu Ala
Gln Gln Arg Cys Asn Leu Asp Asp Thr225 230
235 240Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys
His245 250 255Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu
His Phe Pro260 265 270Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His
Gln Ala Cys His Leu275 280 285Pro Leu Glu Thr Phe Thr Arg His Arg
Gln Pro Arg Gly Trp Glu Gln290 295 300Leu Glu Gln Cys Gly Tyr Pro
Val Gln Arg Leu Val Ala Leu Tyr Leu305 310 315 320Ala Ala Arg Leu
Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala325 330 335Leu Ala
Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu340 345
350Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu
Ser355 360 365Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala
Gly Ala Ala370 375 380Asn Ala Asp Val Val Ser Leu Thr Cys Pro Val
Ala Ala Gly Glu Cys385 390 395 400Ala Gly Pro Ala Asp Ser Gly Asp
Ala Leu Leu Glu Arg Asn Tyr Pro405 410 415Thr Gly Ala Glu Phe Leu
Gly Asp Gly Gly Asp Val Arg His His Phe420 425 430Thr Pro Ser Glu
Arg Gln Leu Cys Leu Ser Ser Ile Gln Thr Ala Phe435 440 445Asn Gln
Gly Ala Gly Thr Cys Ile Leu Ser Asp Ser Gly Arg Ile Ser450 455
460Tyr Thr Val Glu Phe Ser Leu Pro Thr His His Thr Val Arg Leu
Ile465 470 475 480Arg Val Thr Ala Pro Pro Ser Ala Leu Asp Ala Thr
Val Tyr Asn Gly485 490 495Ser Ser Lys Tyr Gly Asp Thr Ser Thr Ser
Asn Val Arg Gly Asp Leu500 505 510Gln Val Leu Ala Gln Lys Ala Glu
Arg Thr Leu Pro Thr Ser Phe Asn515 520 525Phe Gly Ala Ile Lys Ala
Thr Arg Val Thr Glu Phe His Met Val Asp530 535 540Leu Leu Ala Val
Arg Ile Ile Lys Thr Leu Tyr Gln Ser Asn Pro Tyr545 550 555 560Pro
Lys Pro Glu Gly Tyr Arg Arg Val Arg Arg Asn Arg Arg Arg Arg565 570
575Trp Arg Ala Arg Gln Arg Gln Ile His Ser Ile Ser Glu Arg Ile
Leu580 585 590Ile Thr Cys Leu Gly Arg Pro Thr Glu Pro Val Pro Leu
Gln Leu Pro595 600 605Pro Ile Glu Arg Leu Asn Ile Asn Cys Ser Glu
Ser Gly Gly Thr Ser610 615 620Gly Thr Gln Arg Val Gly Asn Pro Leu
Glu Tyr Leu Lys Lys Asp Glu625 630 635 640Leu Arg Val Glu Leu Lys
Asp Glu Leu6452481680DNAArtificial sequenceFusion gene PE(delta
III)-HIV tat-K3 248atgggcagca gccatcatca tcatcatcac agcagcggcc
tggtgccgcg cggcagccat 60atggccgaag aagctttcga cctctggaac gaatgcgcca
aagcctgcgt gctcgacctc 120aaggacggcg tgcgttccag ccgcatgagc
gtcgacccgg ccatcgccga caccaacggc 180cagggcgtgc tgcactactc
catggtcctg gagggcggca acgacgcgct caagctggcc 240atcgacaacg
ccctcagcat caccagcgac ggcctgacca tccgcctcga aggcggcgtc
300gagccgaaca agccggtgcg ctacagctac acgcgccagg cgcgcggcag
ttggtcgctg 360aactggctgg taccgatcgg ccacgagaag ccctcgaaca
tcaaggtgtt catccacgaa 420ctgaacgccg gcaaccagct cagccacatg
tcgccgatct acaccatcga gatgggcgac 480gagttgctgg cgaagctggc
gcgcgatgcc accttcttcg tcagggcgca cgagagcaac 540gagatgcagc
cgacgctcgc catcagccat gccggggtca gcgtggtcat ggcccagacc
600cagccgcgcc gggaaaagcg ctggagcgaa tgggccagcg gcaaggtgtt
gtgcctgctc 660gacccgctgg acggggtcta caactacctc gcccagcaac
gctgcaacct cgacgatacc 720tgggaaggca agatctaccg ggtgctcgcc
ggcaacccgg cgaagcatga cctggacatc 780aaacccacgg tcatcagtca
tcgcctgcac tttcccgagg gcggcagcct ggccgcgctg 840accgcgcacc
aggcttgcca cctgccgctg gagactttca cccgtcatcg ccagccgcgc
900ggctgggaac aactggagca gtgcggctat ccggtgcagc ggctggtcgc
cctctacctg 960gcggcgcggc tgtcgtggaa ccaggtcgac caggtgatcc
gcaacgccct ggccagcccc 1020ggcagcggcg gcgacctggg cgaagcgatc
cgcgagcagc cggagcaggc ccgtctggcc 1080ctgaccctgg ccgccgccga
gagcgagcgc ttcgtccggc agggcaccgg caacgacgag 1140gccggcgcgg
ccaacgccga cgtggtgagc ctgacctgcc cggtcgccgc cggtgaatgc
1200gcgggcccgg cggacagcgg cgacgccctg ctggagcgca actatcccac
tggcgcggag 1260ttcctcggcg acggcggcga cgtcgaattc catatggtcg
accgtgacga actgaaaggt 1320atcggtatgg aaccggttga cccgcgtctg
gaaccgtgga aacacccggg tagccagccg 1380cgtaccgctt gcaacaactg
ctactgcaaa aaatgttgtt tccactgccc ggtttgcttt 1440atctctaaag
gtctgggtat cagctacggt cgtaaaaagc gtcgccagcg ccgccgcgct
1500ccgcaggact ccgaaaccca ccaggttagc ctgagcaagc aaccgaccag
ccagctgcgt 1560ggtgacccga ccggtccgaa agaaagcaaa aaaaaagttg
aacgtgaaac cgaaaccgac 1620ccgaacgttc tcgagtacct caaaaaagac
gaactgcgtg tagaactgaa agacgaactg 1680249560PRTArtificial
sequenceFusion protein PE(delta III)-HIV Tat-K3 249Met Gly Ser Ser
His His His His His His Ser Ser Gly Leu Val Pro1 5 10 15Arg Gly Ser
His Met Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys20 25 30Ala Lys
Ala Cys Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg35 40 45Met
Ser Val Asp Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu50 55
60His Tyr Ser Met Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala65
70 75 80Ile Asp Asn Ala Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg
Leu85 90 95Glu Gly Gly Val Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr
Thr Arg100 105 110Gln Ala Arg Gly Ser Trp Ser Leu Asn Trp Leu Val
Pro Ile Gly His115 120 125Glu Lys Pro Ser Asn Ile Lys Val Phe Ile
His Glu Leu Asn Ala Gly130 135 140Asn Gln Leu Ser His Met Ser Pro
Ile Tyr Thr Ile Glu Met Gly Asp145 150 155 160Glu Leu Leu Ala Lys
Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala165 170 175His Glu Ser
Asn Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly180 185 190Val
Ser Val Val Met Ala Gln Thr Gln Pro Arg Arg Glu Lys Arg Trp195 200
205Ser Glu Trp Ala Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu
Asp210 215 220Gly Val Tyr Asn Tyr Leu Ala Gln Gln Arg Cys Asn Leu
Asp Asp Thr225 230 235 240Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala
Gly Asn Pro Ala Lys His245 250 255Asp Leu Asp Ile Lys Pro Thr Val
Ile Ser His Arg Leu His Phe Pro260 265 270Glu Gly Gly Ser Leu Ala
Ala Leu Thr Ala His Gln Ala Cys His Leu275 280 285Pro Leu Glu Thr
Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln290 295 300Leu Glu
Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu305 310 315
320Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn
Ala325 330 335Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala
Ile Arg Glu340 345 350Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu
Ala Ala Ala Glu Ser355 360 365Glu Arg Phe Val Arg Gln Gly Thr Gly
Asn Asp Glu Ala Gly Ala Ala370 375 380Asn Ala Asp Val Val Ser Leu
Thr Cys Pro Val Ala Ala Gly Glu Cys385 390 395 400Ala Gly Pro Ala
Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro405 410 415Thr Gly
Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Glu Phe His Met420 425
430Val Asp Arg Asp Glu Leu Lys Gly Ile Gly Met Glu Pro Val Asp
Pro435 440 445Arg Leu Glu Pro Trp Lys His Pro Gly Ser Gln Pro Arg
Thr Ala Cys450 455 460Asn Asn Cys Tyr Cys Lys Lys Cys Cys Phe His
Cys Pro Val Cys Phe465 470 475 480Ile Ser Lys Gly Leu Gly Ile Ser
Tyr Gly Arg Lys Lys Arg Arg Gln485 490 495Arg Arg Arg Ala Pro Gln
Asp Ser Glu Thr His Gln Val Ser Leu Ser500 505 510Lys Gln Pro Thr
Ser Gln Leu Arg Gly Asp Pro Thr Gly Pro Lys Glu515 520 525Ser Lys
Lys Lys Val Glu Arg Glu Thr Glu Thr Asp Pro Asn Val Leu530 535
540Glu Tyr Leu Lys Lys Asp Glu Leu Arg Val Glu Leu Lys Asp Glu
Leu545 550 555 56025012PRTArtificial sequenceCarboxyl terminal
moiety 250Lys Lys Asp Leu Arg Asp Glu Leu Lys Asp Glu Leu1 5
1025113PRTArtificial sequenceCarboxyl terminal moiety 251Lys Lys
Asp Glu Leu Arg Asp Glu Leu Lys Asp Glu Leu1 5 1025213PRTArtificial
sequenceCarboxyl terminal moiety 252Lys Lys Asp Glu Leu Arg Val Glu
Leu Lys Asp Glu Leu1 5 10
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