U.S. patent application number 12/273905 was filed with the patent office on 2009-06-04 for invisible antimicrobial glove and hand antiseptic.
This patent application is currently assigned to BECTON, DICKINSON AND COMPANY. Invention is credited to Minh Quang Hoang, Donald Edward Hunt.
Application Number | 20090143470 12/273905 |
Document ID | / |
Family ID | 33098192 |
Filed Date | 2009-06-04 |
United States Patent
Application |
20090143470 |
Kind Code |
A1 |
Hoang; Minh Quang ; et
al. |
June 4, 2009 |
Invisible Antimicrobial Glove and Hand Antiseptic
Abstract
An alcohol based hand surgical scrub, which includes cationic
anti-microbial agent preservatives, cationic polymer film-forming
agents and a skin emollient, and provides a long term residual,
anti-microbial "invisible glove" on the skin. The composition
provides an immediate reduction in bacterial microbes. The polymer
system creates an invisible film on the skin following solvent
evaporation. This invisible film provides a lasting anti-microbial
barrier on the skin which acts to prevent microbial growth.
Inventors: |
Hoang; Minh Quang; (Sandy,
UT) ; Hunt; Donald Edward; (Provo, UT) |
Correspondence
Address: |
David W. Highet, VP & Chief IP Counsel;Becton, Dickinson and Company
(The Webb Firm), 1 Becton Drive, MC 110
Franklin Lakes
NJ
07414-1880
US
|
Assignee: |
BECTON, DICKINSON AND
COMPANY
Franklin Lakes
NJ
|
Family ID: |
33098192 |
Appl. No.: |
12/273905 |
Filed: |
November 19, 2008 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10550184 |
Jul 24, 2006 |
7488757 |
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PCT/US04/09009 |
Mar 24, 2004 |
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12273905 |
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60457054 |
Mar 24, 2003 |
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Current U.S.
Class: |
514/565 |
Current CPC
Class: |
A61K 2800/28 20130101;
A61K 8/43 20130101; A61P 31/00 20180101; A61K 8/922 20130101; A61K
8/416 20130101; A61K 8/731 20130101; A61K 8/86 20130101; B08B 3/04
20130101; A61K 8/342 20130101; A61K 2800/54 20130101; A61Q 19/10
20130101; A61Q 17/005 20130101; A61K 2800/5426 20130101; A61K 8/34
20130101; A61K 8/37 20130101; A61K 8/8176 20130101; A61K 8/46
20130101; A61K 2800/5922 20130101 |
Class at
Publication: |
514/565 |
International
Class: |
A61K 31/195 20060101
A61K031/195; A61P 31/00 20060101 A61P031/00 |
Claims
1. A surgical scrub composition comprising: (a) an alcohol; (b) a
cationic anti-microbial agent; (c) a skin emollient; and (d) a
film-forming cationic polymer system.
2. The surgical scrub composition of claim 1, further comprising a
silicone.
3. The surgical scrub composition of claim 2, wherein the silicone
includes one or more of the group of dimethicone copolyol,
cyclomethicone and dimethicone.
4. The surgical scrub composition of claim 1, further comprising
one or more of the group of methylparaben and propylparaben.
5. The surgical scrub composition of claim 1, wherein the
anti-microbial agent is one or more of the group including
chlorhexidine digluconate, chloroxylenol, parachloro-meta-xylenol,
chlorhexidine diacetate, benzethonium chloride, benzalkonium
chloride, hexetidine, germaben II, kathon CG, triclosan,
hexachlorophenes, octenidine, and quaternary compounds.
6. The anti-microbial composition of claim 1, wherein the polymer
system includes one or more of the group including incroquat
behenyl TMS, incroquat CR concentrate, cellulose,
polyvinylpyrolidone and waxes.
7. A method of disinfecting the skin comprising: (a) applying prior
to surgical procedures an effective amount of a surgical scrub
composition including: i. an alcohol; ii. a cationic anti-microbial
agent; iii. a skin emollient; iv. a film-forming cationic polymer
system; and (b) rubbing the skin with the composition until the
alcohol has evaporated.
8. A method of using a surgical scrub composition for hand
antisepsis comprising: (a) applying a cleansing liquid to skin; (b)
scrubbing the skin with an anti-microbial scrub solution; (c)
rinsing the anti-microbial scrub solution from the skin; and (d)
applying an effective amount of a surgical scrub composition
according to claim 1.
9. The method of claim 8, wherein the surgical scrub composition
includes: the alcohol in an amount from about 60 to about 95 weight
percent of the total composition; the cationic anti-microbial agent
in an amount from about 0.1 to about 5 weight percent of the total
composition; the skin emollient in an amount from about 0.1 to
about 5 weight percent of the total composition; and the
film-forming cationic polymer system in amount from about 0.1 to
about 5 weight percent of the total composition.
10. The method of claim 9, wherein the surgical scrub composition
includes a silicone in an amount from about 0.1 to about 5 weight
percent of the total composition.
11. The method of claim 8, wherein the film-forming polymer system
includes one or more of the group including incroquat behenyl TMS,
incroquat CR concentrate, cellulose, polyvinylpyrolidone and
waxes.
12. The method of claim 8, wherein the effective amount is 20
cc.
13. A surgical scrub composition comprising: (a) one or more
alcohols in an amount from about 60 to about 95 weight percent of
the total composition; (b) one or more film-forming cationic
polymers in an amount from about 0.1 to about 5 weight percent of
the total composition; (c) one or more cationic anti-microbial
agents in an amount from about 0.1 to about 5 weight percent of the
total composition; and (d) one or more skin emollients in an amount
from about 0.1 to about 5 weight percent of the total
composition.
14. The surgical scrub composition of claim 13, further comprising
from about 0.01 to about 5 weight percent of a silicone.
15. (canceled)
16. A surgical scrub composition comprising: (a) a preservative;
(b) chlorhexidine digluconate; (c) a film-forming system including
at least one of the group containing glycerin, methylparaben or
propylparaben; (d) alcohol; and (e) water.
17. A surgical scrub composition comprising: (a) one or more
alcohols selected from the group of ethyl alcohol, isopropyl
alcohol, ethanol and n-propanol; (b) one or more cationic
anti-microbial agents selected from the group of chlorhexidine
digluconate, chloroxylenol, para-chloro-meta-xylenol, chlorhexidine
diacetate, benzethonium chloride, benzalkonium chloride,
hexetidine, germaben II, kathon CG, triclosan, hexachlorophenes,
octenidine and quaternary compounds; (c) a skin emollient; and (d)
a film-forming cationic polymer system including one or more
selected from the group of incroquat behenyl TMS, incroquat CR
concentrate, cellulose, polyvinylpyrolidone, waxes, behenyl
trimonium methosulfate containing compounds and stearalkonium
chloride containing compounds.
18. The surgical scrub composition of claim 17, further comprising
a silicone.
19. The surgical scrub composition of claim 17, further comprising
one or more of methylparaben or propylparaben.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation application of U.S.
patent application Ser. No. 10/550,184, filed Jul. 24, 2006, which
is a national phase of PCT/US04/009009, filed Mar. 24, 2004, which
claims priority from U.S. Provisional Application No. 60/457,054,
filed Mar. 24, 2003.
BACKGROUND OF THE INVENTION
[0002] 1. Field of the Invention
[0003] The present invention relates to an alcohol-based antiseptic
surgical scrub, which leaves an anti-microbial invisible film or
"glove" on the hands, following alcohol evaporation.
[0004] 2. Description of Related Art
[0005] Diligent hand washing and the wearing of gloves by
healthcare professionals are essential components of effective
infection control in the healthcare environment. Healthcare
professionals regularly wash their hands and wear gloves to control
the spread of bacteria and infection from patient to patient, and
to themselves.
[0006] Hand washing procedures are performed in several ways and
include products such as an ordinary anti-microbial bar soap, skin
disinfecting or pre-operative agents or rubbing alcohol. Such
procedures and products contain anti-microbial agents such as
alcohol, iodine, chlorhexidine digluconate, chloroxylenol,
triclosan and hexachlorophenes. Gloving by the healthcare workers
is a normal practice in the hospital setting to create a barrier
that protects themselves and the patient from transmitted
bacteria.
[0007] Healthcare workers commonly use scrub brushes impregnated
with anti-microbial agents for antiseptic hand washing prior to
surgical procedures and other invasive patient care procedures.
These impregnated scrub brushes have proven to be an effective
method of reducing the spread of infection in the healthcare
setting. Anti-microbial solutions are specially designed for use in
the scrub brush where the mechanical action of scrubbing with the
brush creates a foam or lather. Before performing a surgical
procedure, the surgical team uses surgical scrubs to disinfect
their hands with such a standardized scrub procedure, usually
lasting 5 to 10 minutes, then don sterile gloves before
initializing the surgical procedure.
[0008] Alcohols, in general, are recognized for their disinfection
properties. Rubbing alcohol, containing 70% ethyl alcohol (Formula
23-H) and 30% water, and isopropyl rubbing alcohol, containing 70%
isopropyl alcohol and 30% water, are commonly used disinfectants.
Alcohol is a potent anti-microbial agent, and, if used with
surgical scrub, will cause significant mean log reduction of
bacterial counts on the hands of healthcare workers. However, the
use of alcohols for surgical scrubs has never become popular in the
United States of America, even though the compound has very good
properties for immediate reduction of microbial flora on the hands.
This may be due to the fact that alcohol is a defatting agent. When
applied to the human skin, it can make the skin very dry, often
leading to chapped and cracked skin. These characteristics thus
often result in difficult and painful sensitivity for the
users.
[0009] The prior art includes many examples of varying antiseptic
skin cleaners and wipes, some of which include alcohol. U.S. Pat.
No. 4,678,606 discloses a personal liquid cleaning composition
having a primary surfactant selected from the group of anionic,
cationic, zwitterionic, amphoteric and semipolar surfactants, an
auxiliary surfactant selected from certain ethoxylated aliphatic
alcohols and a water-soluble polymeric thickening agent. U.S. Pat.
No. 4,374,126 discloses an alcohol insoluble antimicrobial topical
lotion including a lower acrylate interpolymer. U.S. Pat. No.
5,512,199 discloses an antimicrobial hand wipe which includes an
alcohol. U.S. Pat. No. 6,180,584 is directed to a film forming
antimicrobial composition. Notably, this composition is an
alcohol-free anti-microbial skin sanitizing composition.
[0010] However, none of these prior art products provide the
benefits of the presently disclosed surgical scrub composition,
which not only includes alcohol, but also provides the desired
invisible, anti-microbial long-lasting film on the hands following
solvent evaporation.
SUMMARY OF THE INVENTION
[0011] The present invention is an alcohol based hand surgical
scrub, which includes dual anti-microbial agent preservatives,
cationic polymer film-forming agents and provides a long term
residual, anti-microbial "invisible glove" on the skin. The
composition desirably comprises an alcohol, anti-microbial agent
which prevents the growth of bacteria and a polymer system to form
a barrier on the skin. The polymer system creates an invisible film
on the skin following solvent evaporation. This invisible film
provides a lasting anti-microbial barrier on the skin.
[0012] The composition desirably includes an alcohol, a positive
charged anti-microbial agent, skin emollients and lubricants and a
film-forming polymer system. The composition may also include one
or both of methyl and propyl parabens and silicone. The
anti-microbial agents prevent bacteria from growing back to the
baseline of the normal skin flora population. The surgical scrub
composition contains chemical ingredients to remove extraneous
matter and organisms from the hands. Skin emollients and lubricants
are also provided to prevent the defatting effect of the alcohol,
thereby eliminating the dry, chapped feeling on the skin.
Additional or complimentary anti-microbial agents are preferably
included to serve as preservative and persistent active agents.
These anti-microbial agents in the composition will improve the
anti-microbial characteristics of the formulation and provide a
long-term residual activity for the skin. As previously noted, the
polymer system is included to create an invisible film after the
solvent is evaporated. This invisible film acts as a physical
anti-microbial barrier or "glove" to protect the healthcare
worker's hands. This film has an anti-microbial effect at least for
six hours to prevent and kill the bacteria on the healthcare
worker's hands.
BRIEF DESCRIPTION OF THE DRAWING
[0013] FIG. 1 is a graphical presentation of the log.sub.10
reductions from baseline for the preferred formula and BD E-Z
Scrub.TM. [BD Catalog #371073] plus the most preferred
embodiment.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0014] This invention may be satisfied by embodiments in many
different forms, and is not limited to any specific or preferred
embodiments of the invention, which are merely exemplary of the
principles of the invention. Various other modifications will be
apparent to and readily made by those skilled in the art without
departing from the scope and spirit of the invention. The scope of
the invention will be measured by the appended claims and their
equivalents.
[0015] The surgical scrub composition of the present invention
includes alcohol, a positive-charged anti-microbial agent, skin
emollients and lubricants, and a cationic polymer film-forming
thickening agent.
[0016] One or more alcohols are utilized in the surgical scrub
composition due to alcohol's well known germicidal properties.
Alcohols which may be used in the present composition include
isopropyl alcohol, ethanol, and n-propanol. In the most preferred
embodiment, ethyl alcohol is used. Preferably, alcohol is present
in the surgical scrub composition in an amount from about 60 to
about 95 weight percent, and most preferably, at about 71 weight
percent.
[0017] Emollients are utilized in the surgical scrub composition of
the present invention to lessen the drying effect of the alcohol on
the skin. A preferred emollient is isopropyl palmitate, which is an
ester emollient available from Amerchol Corporation of Edison, N.J.
When used on the skin, isopropyl palmitate leaves a soft,
non-sticky feel to the skin. Isopropyl palmitate is a lipophillic
emollient, an oily, fatty substance which is water insoluble.
Lanolin and its derivatives are a large group of fatty materials
with active emollient properties that can be utilized as
emulsifiers or lubricants in the composition.
[0018] The preferred embodiment includes glycerin as a hydrophilic
emollient and a humectant to maintain the water in equilibrium.
Glycerin is available from Spectrum Quality products. Glycerin is a
water and alcohol soluble emollient that provides skin conditioning
properties to the composition. In another embodiment, propylene
glycol may be substituted for glycerin. Preferably, glycerin is
present in the surgical scrub composition, by weight percent of the
entire composition, in an amount of from about 0.1% to about 5%,
and most preferably, about 1.4%.
[0019] The surgical scrub composition further includes a silicone
fluid. Preferably, the silicone fluid is dimethicone copolyol,
cyclomethicone and dimethicone. Most preferably, dimethicone is
utilized. Dimethicone 350 is commercially available from General
Electric Corporation. Silicone fluids provide good lubricating,
aesthetic and occlusive action on the skin. Silicone fluids not
only lubricate the skin surface, they also act as a gas barrier to
reduce water vapor transmission. Silicone fluids are water and gas
insoluble. Preferably, the silicone fluid is present in the
surgical scrub composition, by weight percent of the entire
composition, in an amount from 0 to about 5%, and most preferably,
about 0.03%.
[0020] Most hydrocarbons are derived from petrolatum crude by a
fractional distillation process. Such hydrocarbons function as
emollients due to their ability to lubricate and hold water at the
skin surface due to their relative occlusivity. Mineral oil and
isopropyl palmitate are included in the most preferred embodiment.
Despite its lack of aesthetic properties, petrolatum may be
utilized. Alternatively or additionally, lanolin derivatives may be
included. Preferably, mineral oil is present in the surgical scrub
composition, by weight percent of the entire composition, in an
amount from 0 to about 5%, and most preferably, about 0.2%.
Preferably, isopropyl palmitate is present in the surgical scrub
composition, by weight percent of the entire composition, in an
amount from about 0.1% to about 5%, and most preferably about
0.4%.
[0021] Preferably, the surgical scrub composition includes about
0.1% to about 5% of an emollient or emollients.
[0022] An anti-microbial agent is included in the surgical scrub
composition to kill microorganisms and prevent or inhibit their
growth and reproduction. In the absence of an antimicrobial agent,
microbial flora will grow on the skin following alcohol
evaporation. The antimicrobial agents present in the surgical scrub
composition are selected to be compatible with the chemical and
physical properties of the skin. In addition, the antimicrobial
agent should be non-toxic and environmentally friendly.
[0023] As previously noted, the alcohol component of the surgical
scrub composition is an excellent antimicrobial agent. It also acts
as a preservative. However, following application of the surgical
scrub composition, the alcohol will evaporate from the skin over
time. In the most preferred embodiment, the residue remaining on
the skin following alcohol evaporation will include an amount of an
antimicrobial agent which also acts as a preservative. Suitable
antimicrobial agents which act as preservatives may be selected
from the class of phenols including para-chloro-meta-xylenol,
bis-biguanides such as chlorhexidine digluconate, chlorhexidine
diacetate or quaterium class such as benzethonium chloride,
benzalkonium chloride. Chloroxylenol, triclosan, hexachlorophenes,
octenidine and quarternary compounds may be included. Hexetidine,
germaben II, kathon CG, triclosan are other antimicrobial agents
may also be suitable as preservatives. Benzethonium chloride and
benalkonium chloride (as hyamine 3500) are available from Lonza
Inc., chlorhexidine digluconate is available from Xttrium
Laboratories, gennaben II is available from Sutton Laboratories. In
a most preferred embodiment, benzethonium chloride, chlorhexidine
digluconate (20%) and benzelkonium chloride (80%) are present.
Preferably, benzethonium chloride is present in the scrub
composition, by weight percent of the entire composition, in an
amount from about 0.05% to about 5%, and most preferably, about
0.09%. Preferably, chlorhexidine digluconate (20%) is present in
the surgical scrub composition, by weight percent of the entire
composition, in an amount from about 0.05% to about 5%, and most
preferably about 0.45%. Preferably, benzalkonium chloride (80%) is
present in the surgical scrub composition, by weight percent of the
entire composition, in an amount from 0 to about 5%, and most
preferably, about 0.09%. Preferably, the composition includes about
0% to about 5% of an anti-microbial agent.
[0024] Methylparaben and propylparaben (both available from
Mallinckrodt Chemical Company) are desirably included in the
surgical scrub composition as preservatives. These are further
desired for their moisturizing and film forming characteristics.
Preferably, methylparaben is contained in the surgical scrub
composition, by weight percent of the entire composition, in an
amount from about 0 to about 5%, and most preferably about 0.03%.
Preferably, propylparaben is present in the surgical scrub, by
weight percent of the entire composition, in an amount from about 0
to about 5%, and most preferably, about 0.03%. One or both of these
compositions are preferably included in the amount of about 0% to
about 5% of the surgical scrub composition.
[0025] To create the desired residual film of the surgical scrub
composition, cationic polymer thickening agents are employed.
Thickening agents must be soluble in the alcohol and compatible
with cationic ingredients. The cationic polymer thickening agents
are preferably present in the surgical scrub composition, by weight
percent of the entire composition, in the amount of about 0.1% to
about 5%.
[0026] Incroquat behenyl TMS is most preferably used as a cationic
polymer. It is a strong conditioning agent and an outstanding
cationic emulsifier. This composition bonds to the skin due to the
skin's negative charge. Incroquat behenyl TMS is a compound of 25%
active solution of behenyl trimonium methosulfate in cetearyl
alcohol and is available in flaked/pastel form and supplied by
Croda Inc. Incroquat CR concentrate is preferably used as an
additional cationic polymer. Incroquat CR concentrate is available
from Croda me. and is comprised of cetearyl alcohol, castor oil and
stearalkonium chloride. This composition is a one-part formulating
aid. It is a conditioner and self-emulsifier. The desired
combination of the incroquat behenyl TMS and incroquat CR
concentrate provides a smooth feel to the skin and neutralizes the
static charge of the human skin. Preferably, incroquat BTMS is
present in the surgical scrub composition, by weight percent of the
entire composition, in an amount from about 0.1% to about 5%, and
most preferably about 0.4%. Preferably, incroquat CR is present in
the surgical scrub composition, by weight percent of the entire
composition, in an amount from 0 to about 5%, and most preferably
about 0.1%.
[0027] Alternative or additional polymers include cellulose,
polyvinylpyrolidone and waxes.
[0028] The most preferred embodiment of the surgical scrub
composition is as follows:
TABLE-US-00001 % of Total (w/w) 1-Docosanol 1.00% Benzethonium
Chloride 0.09% Chlorhexidine Digluconate, (20%) 0.45% Dimethicone
0.03% Ethyl Alcohol 71.00% Glycerin 1.40% Benzalkonium Chloride
0.09% Incroquat BTMS 0.40% Incroquat CR 0.10% Isopropyl Palmitate
0.40% Mineral Oil 0.20% Methylparaben 0.03% PEG-10 Behenyl Ether
1.50% Propylparaben 0.03% Purified Water 23.28%
[0029] The most preferred embodiment includes the additional
components 1-Docosanol as a moisturizer and film forming substance,
PEG-10 behenyl ether as a moisturizer, fragrance and water.
Preferably, 1-Docosanol is present in the surgical scrub
composition, by weight percent of the entire composition, in an
amount from about 0.1% to about 5%, and most preferably about 1%.
Preferably, PEG-10 behenyl ether is present in the surgical scrub
composition, by weight percent of the entire composition, in an
amount from about 0.1% to about 5%, and most preferably about 1.5%.
Preferably, purified water is present in the surgical scrub
composition, in an amount from about 1% to about 35%, and most
preferably about 23%.
[0030] The surgical scrub composition does not cause skin
irritation or sensitization. Additional advantages of the
composition include its compatibility with positive-charged
anti-microbial agents such as chlorhexidine digluconate, thus
ensuring the effectiveness of the anti-microbial agent or agents.
The included skin emollients and lubricants eliminate skin drying
or chapping. These compositions counteract the defatting effect of
the alcohol.
[0031] The surgical scrub composition includes anti-microbial
agents, which act as both persistent active agents and suitable
preservatives. This composition permits a very small amount of an
anti-microbial agent to improve anti-microbial activities and
provide long-term residual activity for the skin.
[0032] Traditionally, healthcare workers have scrubbed prior to
every surgical procedure. An improved method may be to apply water
to the skin, perform hand scrubbing at the beginning of the day
using a scrub brush and anti-microbial scrub solution, rinsing the
anti-microbial scrub solution with water, and then utilization of
the composition of the present invention prior to subsequent
surgical procedures. A high level of hand antisepsis can be
achieved via this method without abrasive scrubbing multiple times
a day.
EXAMPLES
[0033] The following examples are not intended to limit the scope
of the invention, but are only intended to be exemplary in
nature.
[0034] Standard testing of the most preferred embodiment
demonstrates its effectiveness as an antiseptic to provide an
immediate reduction of microbes and then to prevent microbial
growth back to baseline within a 6-hour period. The immediate log
reduction was 2.65 on day 1, 2.92 on day 2 and 3.04 on day 3. The
residual effect was a log reduction six hours after hand cleaning
of 1.45 on day 1, 1.79 on day 2 and 2. 18 on day 3. The residual
anti-microbial activity is facilitated by the containment of the
anti-microbial agent in the polymer film that is formed. The film
forms an invisible "glove" containing an anti-microbial agent. The
anti-microbial agent remains on the skin in the film and continues
to be bactericidal over extended periods of time. The invisible
glove prevents microbes that may be present on the hands of the
healthcare worker from infecting a patient and the glove also
provides a barrier for microbes infecting the healthcare worker
from the patient.
[0035] Through testing, the composition has been shown to exceed
industry standards. The critical indices for this study were an
immediate one (1) log.sub.10 reduction in microorganisms on Day 1;
an immediate two (2) log.sub.10 reduction in microorganisms on Day
2; an immediate three (3) log.sub.10 reduction in microorganisms on
Day 5; and that microorganism populations from the approximately
three (3) hour to three (3) hour and thirty (30) minute AND
approximately six (6) hour to six (6) hour and thirty (30) minute
samples not return to baseline level. Refer to Table I.
TABLE-US-00002 TABLE I Statistical Summary of the Log.sub.10
Recovery Values of Most Preferred Embodiment Stan- Log.sub.10 TFM
(1994) Sam- dard 95% Reduction Required ple Devi- Confidence from
Log.sub.10 Sample Size Mean ation Interval Baseline Reduction
Baseline 36 6.01 0.40 5.88 to N/A 6.15 Day 1, 12 3.36 0.68 2.93 to
2.65 1 Immediate 3.79 Day 1, 3 12 4.08 0.99 3.45 to 1.93 No return
to Hour 4.71 baseline Day 1, 6 12 4.56 0.68 4.13 to 1.45 No return
to Hour 4.99 baseline Day 2, 11 3.09 0.53 2.74 to 2.92 2 Immediate
3.45 Day 2, 3 11 3.73 0.90 3.12 to 2.28 No return to Hour 4.34
baseline Day 2, 6 12 4.22 0.81 3.70 to 1.79 No return to Hour 4.73
baseline Day 5, 10 2.97 0.47 2.63 to 3.04 3 Immediate 3.31 Day 5, 3
11 3.50 0.61 3.09 to 2.51 No return to Hour 3.91 baseline Day 5, 6
11 3.83 1.01 3.15 to 2.18 No return to Hour 4.51 baseline
[0036] The most preferred embodiment was also tested in conjunction
with a standard hand scrub utilizing a brush impregnated with an
anti-microbial agent. The composition was applied to the hands of
the test subject one-half hour following the standard scrub. Test
results showed an increased log reduction of microbes when the
scrubbing and the preferred composition were used together. Log
reductions of microbes were 3.66 for Day 1, 3.98 for Day 2, and
3.70 for Day 3. Use of the preferred composition in conjunction
with a standard scrub exceeded industry standards for
anti-microbial compositions. Refer to Table II and FIG. 1.
TABLE-US-00003 TABLE II Statistical Summary of the Log.sub.10
Recovery Values Using BD E-Z Scrub Catalog #371073 Plus Most
Preferred Embodiment Sample Stan- Log.sub.10 TFM (1994) Sam- dard
95% Reduction Required ple Devi- Confidence from Log.sub.10 Sample
Size Mean ation Interval Baseline Reduction Baseline 38 6.10 0.38
5.97 to N/A 6.22 Day 1, 12 2.88 0.46 2.15 to 3.66 1 Immediate 2.73
Day 1, 3 13 3.13 0.79 2.65 to 2.97 No return Hour 3.60 baseline Day
1, 6 13 3.79 0.88 3.26 to 2.31 Hour 4.32 Day 2, 12 2.12 0.35 1.90
to 3.98 2 Immediate 2.34 Day 2, 3 12 2.58 0.93 1.99 to 3.52 No
return Hour 3.17 baseline Day 2, 6 12 2.58 0.66 2.16 to 3.52 Hour
3.00 Day 5, 10 2.29 0.37 2.02 to 3.81 3 Immediate 2.55 Day 5, 3 10
2.28 0.32 2.05 to 3.82 No return Hour 2.51 baseline Day 5, 6 12
2.40 0.49 2.09 to 3.70 Hour 2.71
* * * * *