U.S. patent application number 12/139151 was filed with the patent office on 2008-12-18 for self assembled films for protein and drug delivery applications.
This patent application is currently assigned to Massachusetts Institute of Technology. Invention is credited to Paula T. Hammond, Mara L. Macdonald.
Application Number | 20080311177 12/139151 |
Document ID | / |
Family ID | 40132561 |
Filed Date | 2008-12-18 |
United States Patent
Application |
20080311177 |
Kind Code |
A1 |
Hammond; Paula T. ; et
al. |
December 18, 2008 |
Self Assembled Films for Protein and Drug Delivery Applications
Abstract
Provided are systems for controlled release of proteins from
decomposable thin films constructed by layer-by-layer deposition.
Such films generally comprise alternating layers of polymers and
proteins, and may further comprise additional layers of polyions.
In some embodiments, decomposable thin films and methods of using
such films allow proteins to be released over an extended period of
time and/or retention of as much as 100% of function of released
protein.
Inventors: |
Hammond; Paula T.; (Newton,
MA) ; Macdonald; Mara L.; (Arvada, CO) |
Correspondence
Address: |
Patent Department;Attn: Sam Pasternack, Esq.
Choate, Hall & Stewart, LLP, Two International Place, Suite 34
Boston
MA
02110
US
|
Assignee: |
Massachusetts Institute of
Technology
Cambridge
MA
|
Family ID: |
40132561 |
Appl. No.: |
12/139151 |
Filed: |
June 13, 2008 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
60943983 |
Jun 14, 2007 |
|
|
|
Current U.S.
Class: |
424/443 ;
428/478.2 |
Current CPC
Class: |
A61K 38/1825 20130101;
A61K 38/1866 20130101; A61L 2420/02 20130101; A61L 2420/08
20130101; A61P 43/00 20180101; A61L 33/08 20130101; A61K 31/726
20130101; A61K 38/00 20130101; A61K 38/1875 20130101; A61L 2300/252
20130101; A61K 31/727 20130101; A61L 31/148 20130101; Y10T
428/31768 20150401; B82Y 30/00 20130101; A61L 33/0011 20130101;
A61L 27/54 20130101; A61L 27/58 20130101; A61L 2300/608 20130101;
A61K 9/7007 20130101; A61L 31/10 20130101; A61L 2300/604 20130101;
A61L 27/34 20130101; A61L 2300/42 20130101; A61L 31/16
20130101 |
Class at
Publication: |
424/443 ;
428/478.2 |
International
Class: |
A61K 9/70 20060101
A61K009/70; B32B 9/02 20060101 B32B009/02; A61P 43/00 20060101
A61P043/00 |
Goverment Interests
GOVERNMENT FUNDING
[0002] The work described herein was supported, in part, by grants
from the National Institutes of Health (1-R01-AG029601-01) and a
National Science Foundation Graduate Research Fellowship to Mara
Macdonald. The work described herein made use, in part, of MRSEC
Shared Facilities supported by the National Science Foundation
under Award Number DMR-0213282. The Government of the United States
has certain rights in this invention.
Claims
1. A decomposable thin film comprising: a plurality of multilayer
units comprising a first layer having a first charge and a second
layer having a second charge, wherein: at least a portion of the
multilayers comprise a protein, decomposition of the thin film is
characterized by sequential removal of at least a portion of the
layers having the first charge and degradation of layers having the
second charge and by release of the protein from the corresponding
layer, and the decomposable thin film comprises at least one
degradable polyelectrolyte layer, wherein the degradable
polyelectrolyte is hydrolyzable.
2. The decomposable thin film of claim 1, wherein: the thin film
comprises alternating cationic and anionic layers, and
decomposition of the thin film is characterized by hydrolytic
degradation of at least a portion of a layer selected from the
group consisting of the cationic layers, the anionic layers, and
both.
3. The decomposable thin film of claim 1, wherein at least a
portion of the degradable polyelectrolyte layers comprises a
polyelectrolyte selected from the group consisting of a synthetic
polyelectrolyte, a natural polyelectrolyte, and both.
4. The decomposable thin film of claim 1, wherein at least a
portion of the degradable polyelectrolyte layers comprises a
polymer selected from polyesters, polyanhydrides, polyorthoesters,
polyphosphazenes, polyphosphoesters, and combinations thereof.
5. The decomposable thin film of claim 4, wherein the polymer is a
polyester selected from the group consisting of poly(.beta.-amino
ester)s, poly(L-lactide-co-L-lysine), poly(serine ester),
poly(4-hydroxy-L-proline ester), poly[-(4-aminobutyl)-L-glycolic
acid], and any combination thereof.
6. The decomposable thin film of claim 5, wherein the polyester is
a poly(.beta.-amino ester) selected from the group consisting of
##STR00002## wherein: linker A and linker B are each independently
selected from the group consisting of carbon chains of 1 to 30
carbon atoms, heteroatom-containing carbon chains of 1 to 30 atoms,
and carbon chains and heteroatom-containing carbon chains with at
least one substituent selected from the group consisting of
branched and unbranched alkyl, branched and unbranched alkenyl,
branched and unbranched alkynyl, amino, alkylamino, dialkylamino,
trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic,
cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide,
carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl,
alkylthioether, and thiol groups; R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
branched and unbranched alkyl, branched and unbranched alkenyl,
branched and unbranched alkynyl, aryl, halogen, hydroxyl, alkoxy,
carbamoyl, carboxyl ester, carbonyldioxyl, amide, thiohydroxyl,
alkylthioether, amino, alkylamino, dialkylamino, trialkylamino,
cyano, ureido, a substituted alkanoyl group, cyclic, cyclic
aromatic, heterocyclic, and aromatic heterocyclic groups, each of
which may be substituted with at least one substituent selected
from the group consisting of branched and unbranched alkyl,
branched and unbranched alkenyl, branched and unbranched alkynyl,
amino, alkylamino, dialkylamino, trialkylamino, aryl, ureido,
heterocyclic, aromatic heterocyclic, cyclic, aromatic cyclic,
halogen, hydroxyl, alkoxy, cyano, amide, carbamoyl, carboxylic
acid, ester, carbonyl, carbonyldioxyl, alkylthioether, and thiol
groups; and n is an integer greater than or equal to 5.
7. The decomposable thin film of claim 6, wherein the
poly(.beta.-amino ester) is ##STR00003##
8. The decomposable thin film of claim 7, wherein the
poly(.beta.-amino ester) is ##STR00004##
9. The decomposable thin film of claim 7, wherein the
poly(.beta.-amino ester) is ##STR00005##
10. The decomposable thin film of claim 1, wherein at least a
portion of the degradable polyelectrolyte layers comprises a
polymer selected from poly(styrene sulfonate), poly(acrylic acid),
linear poly(ethylene imine), poly(diallyl dimethyl ammonium
chloride), poly(allylamine hydrochloride), and combinations
thereof.
11. The decomposable thin film of claim 1, wherein the degradation
is characterized by at least one characteristic selected from the
group consisting of hydrolytic, thermal, enzymatic, and
photolytic.
12. The decomposable thin film of claim 1, wherein at least a
portion of the degradable polyelectrolyte layers comprises a
biodegradable polymer.
13. The decomposable thin film of claim 12, wherein the
biodegradable polymer is selected from polyhydroxyacids;
polypropylfumarates; polycaprolactones; polyamides; poly(amino
acids); polyacetals; polyethers; biodegradable polycyanoacrylates;
biodegradable polyurethanes; polysaccharides; and co-polymers,
mixtures, and adducts thereof.
14. The decomposable thin film of claim 1, wherein the thin film is
adapted and constructed as a hollow shell.
15. The decomposable thin film of claim 1, wherein the thin film is
deposited on a non-planar substrate.
16. The decomposable thin film of claim 15, wherein the thin film
is deposited on a substrate having a shape selected from particles,
tube, sphere, strand, coiled strand, and capillary network.
17. The decomposable thin film of claim 15, wherein degradation of
the thin film enables dissolution of the substrate material.
18. The decomposable thin film of claim 15, wherein the substrate
comprises a drug.
19. The decomposable thin film of claim 1, wherein the thin film is
disposed on a substrate, wherein the surface properties of the
substrate vary across a surface of the substrate.
20. The decomposable thin film of claim 1, wherein the thin film is
disposed on a substrate comprising a material selected from metals,
metal oxides, plastics, ceramics, silicon, glasses, mica, graphite,
hydrogels, polymers, and any combination thereof.
21. The decomposable thin film of claim 1, wherein the protein is a
growth factor.
22. The decomposable thin film of claim 21, wherein the growth
factor is selected from the group consisting of vascular
endothelial growth factor (VEGF), bone morphogenic protein 2
(BMP-2), bone morphogenic protein 4 (BMP-4), and basic fibroblast
growth factor (bFGF).
23. The decomposable thin film of claim 1, wherein the protein is a
clotting factor.
24. The decomposable thin film of claim 23, wherein the clotting
factor is selected from the group consisting of factor I
(fibrinogen), factor II (prothrombin), factor III (tissue
thromboplastin), factor V (proaccelerin), factor VII (stable
factor), factor VIII (antihemophilic factor), factor IX (plasma
thromboplastin component), factor X (thrombokinase), factor XI
(plasma thromboplastin antecedent), factor XII (Hageman factor),
and factor XIII (fibrin stabilizing factor).
25. The decomposable thin film of claim 1, wherein the protein is
an enzyme polypeptide.
26. The decomposable thin film of claim 25, wherein the enzyme
polypeptide is lysozyme.
27. The decomposable thin film of claim 1, wherein the multilayer
units comprise bilayer units of the structure (polyelectrolyte
layer having the first charge/protein layer having the second
charge).
28. The decomposable thin film of claim 1, wherein the multilayer
units each further comprise two layers, each layer having either
the first charge or the second charge; and each layer within the
multilayer unit is adjacent to a layer having a different
charge.
29. The decomposable thin film of claim 28, wherein the multilayer
units comprise tetralayer units of the structure (polyelectrolyte
having the first charge/first biopolymer having the second
charge/protein layer having the first charge/second biopolymer
having the second charge).
30. The decomposable thin film of claim 29, wherein the first
biopolymer and the second biopolymer are the same.
31. The decomposable thin film of claim 29, wherein at least one of
the biopolymers is a polysaccharide.
32. The decomposable thin film of claim 31, wherein the
polysaccharide is selected from the group consisting of heparan
sulfate, chondroitin sulfate, hyaluronic acid, and dextran
sulfate.
33. The decomposable thin film of claim 1, wherein the film
comprises at least 10 multilayer units.
34. The decomposable thin film of claim 33, wherein the film
comprises at least 20 multilayer units.
35. The decomposable thin film of claim 34, wherein the film
comprises at least 30 multilayer units.
36. The decomposable thin film of claim 35, wherein the film
comprises at least 40 multilayer units.
37. The decomposable thin film of claim 36, wherein the film
comprises at least 50 multilayer units.
38. The decomposable thin film of claim 37, wherein the film
comprises at least 60 multilayer units.
39. The decomposable thin film of claim 38, wherein the film
comprises at least 70 multilayer units.
40. The decomposable thin film of claim 39, wherein the film
comprises at least 80 multilayer units.
41. A method of releasing a protein from a thin film comprising:
providing a decomposable thin film comprising a plurality of
multilayers including a first layer having a first charge and a
second layer having a second change, wherein the protein is
associated with at least one of the layers and the decomposable
thin film includes at least one degradable polyelectrolyte layer,
wherein the degradable polyelectrolyte is hydrolyzable; and placing
the thin film in a medium in which at least a portion of the thin
film decomposes via the substantially sequential removal of at
least a portion of the layers having the first charge and
degradation of layers having the second charge.
42. The method of claim 41, wherein the protein is released from
the thin film with linear kinetics over a period of at least 5
days.
43. The method of claim 41, wherein the protein is released from
the thin film with linear kinetics over a period of at least 10
days.
44. The method of claim 41, wherein the protein is released from
the thin film with linear kinetics over a period of at least 14
days.
45. The method of claim 41, wherein the protein continues to be
released from the thin film over a period of at least 5 days.
46. The method of claim 45, wherein the protein continues to be
released from the thin film over a period of at least 10 days.
47. The method of claim 46, wherein the protein continues to be
released from the thin film over a period of at least 15 days.
48. The method of claim 47, wherein the protein continues to be
released from the thin film over a period of at least 20 days.
49. The method of claim 48, wherein the protein continues to be
released from the thin film over a period of at least 25 days.
50. The method of claim 49, wherein the protein continues to be
released from the thin film over a period of at least 30 days.
51. The method of claim 50, wherein the protein continues to be
released from the thin film over a period of at least 34 days.
52. The method of claim 41, wherein protein released from the thin
film has at least 50% activity after release from the thin
film.
53. The method of claim 52, wherein protein released from the thin
film has at least 60% activity after release from the thin
film.
54. The method of claim 53, wherein protein released from the thin
film has at least 70% activity after release from the thin
film.
55. The method of claim 54, wherein protein released from the thin
film has at least 80% activity after release from the thin
film.
56. The method of claim 55, wherein the protein released from the
thin film has at least 90% activity after release from the thin
film.
Description
RELATED APPLICATIONS
[0001] This application claims priority to and claims benefit of
U.S. Provisional Application No. 60/943,983 filed Jun. 14, 2007,
the entire contents of which are herein incorporated by
reference.
BACKGROUND
[0003] Many proteins are potentially useful in therapeutic drug
applications. Nevertheless, controlled delivery of proteins remains
a challenge, due in part to the fragile nature of some proteins
(such as enzyme polypeptides) and the ability of proteins to
diffuse. Sustained delivery of proteins while maintaining function
is particularly desirable.
[0004] Layer-by-layer (LbL) absorption of oppositely charged
polyelectrolytes on substrates can be used to fabricate thin
multi-layer films for drug development. Nevertheless, LbL-based
methods of delivering drugs were traditionally based on the
formation of uniform films from which drug escapes via diffusion.
Such diffusion-based release limits or eliminates the opportunity
for controlled sequential delivery of drugs released from the
surface to the surrounding medium. With such films, a typical
diffusive, nonlinear drug release pattern is observed, and rarely
is diffusion-controlled release from LbL films sustained for more
than a few hours.
[0005] Because release time is impacted by the affinity of the drug
for water, it is directly related to the hydrophobic nature of the
drug, rather than an externally controlled parameter. Thus,
releasing drugs by diffusion is not a useful strategy for all
hydrophilic drugs, such as proteins. Other methods allow
encapsulation of proteins within a shell of LbL coats for release
under significant pH (pH 8 or higher) or ionic strength changes,
nevertheless, this is impractical for many medical applications, as
such large deviations from physiological conditions would be often
deadly. Also, processing methods for such films typically involve
harsh solvents, in addition to acidic byproducts of degradation,
which may destroy the protein intended to be delivered.
SUMMARY
[0006] In various embodiments, the invention provided systems for
controlled release of proteins while preserving protein function
using LbL deposition to incorporate proteins into decomposable thin
films.
[0007] In one aspect, the invention provides decomposable thin
films for releasing proteins. Such decomposable thin films
generally comprise a plurality of multilayer units comprising a
first layer having a first charge and second layer having a second
charge. At least a portion of the multilayers comprise a protein
and decomposition of the thin film is characterized by sequential
removal of at least a portion of the layers having the first charge
and degradation of layers having the second charge, and by release
of the protein from the corresponding layer. The decomposable thin
film comprises at least one degradable polyelectrolyte layer,
wherein the degradeable polyelectrolyte is hydrolyzable. Erosion of
the polyelectrolyte layer allows release of the protein.
[0008] In certain embodiments, the decomposable thin film comprises
alternating cationic and anionic layers, and decomposition of the
thin film is characterized by hydrolytic degradation of at least a
portion of a layer (such as a cationic layer, an anionic layer, or
both). In some embodiments of the invention, the decomposable thin
film is comprised of tetralayer units, with each tetralayer having
a structure such as, for example, (cationic degradable
polymer/polyanion/cationic protein/polyanion). Other structures are
also contemplated in the invention. The protein can be any of a
number of proteins, for example, growth factors, clotting factors,
enzyme polypeptides, etc.
[0009] In certain embodiments, proteins released from films of the
invention can be released in a controlled manner, for example, with
a linear release profile. Such films with linear release profiles
may be amenable to therapeutic drug applications. Dosing and
release kinetics may be altered by altering one or more
characteristics such as, for example, the degradable polymer used
in film construction, the number of multilayers comprising the
film, and/or the type of additional materials (such as polyanions)
that are used in the construction of the films. Additional film
properties such as anticoagulant activity or providing matrix
material for cell proliferation can be chosen through the polyanion
used. Examples of polyanions that can be used in accordance with
the invention include charged polysaccharides such as heparin and
chondroitin.
[0010] The thin film can be adapted or shaped and/or deposited onto
substrates having certain shapes. This may facilitate making such
films amenable for drug applications, such as, for example, those
in which the films would be implanted into a patient's body. For
example, the film may be constructed as a hollow shell, or
deposited onto substrates having various shapes.
[0011] In another aspect, the invention provides methods of
releasing proteins using decomposable thin films of the
invention.
[0012] In certain embodiments, films and methods provided in the
invention allow protein release over a period of at least 34 days
and/or up to 80-100% retention of function of protein released from
such films.
DEFINITIONS
[0013] "Biomolecules": The term "biomolecules", as used herein,
refers to molecules (e.g., proteins, amino acids, peptides,
polynucleotides, nucleotides, carbohydrates, sugars, lipids,
nucleoproteins, glycoproteins, lipoproteins, steroids, etc.)
whether naturally-occurring or artificially created (e.g., by
synthetic or recombinant methods) that are commonly found in cells
and tissues. Specific classes of biomolecules include, but are not
limited to, enzymes, receptors, neurotransmitters, hormones,
cytokines, cell response modifiers such as growth factors and
chemotactic factors, antibodies, vaccines, haptens, toxins,
interferons, ribozymes, anti-sense agents, plasmids, DNA, and
RNA.
[0014] "Biocompatible": The term "biocompatible", as used herein is
intended to describe materials that do not elicit a substantial
detrimental response in vivo.
[0015] "Biodegradable": As used herein, "biodegradable" polymers
are polymers that degrade fully under physiological or endosomal
conditions. In preferred embodiments, the polymers and
biodegradation byproducts are biocompatible. Biodegradable polymers
are not necessarily hydrolytically degradable and may require
enzymatic action to fully degrade.
[0016] "Degradation": The phrase "degradation", as used herein,
relates to the cleavage of a covalent polymer backbone. Full
degradation of a polymer breaks the polymer down to monomeric
species.
[0017] "Endosomal conditions": The phrase "endosomal conditions",
as used herein, relates to the range of chemical (e.g., pH, ionic
strength) and biochemical (e.g., enzyme concentrations) conditions
likely to be encountered within endosomal vesicles. For most
endosomal vesicles, the endosomal pH ranges from about 5.0 to
6.5.
[0018] "Hydrolytically degradable": As used herein, "hydrolytically
degradable" polymers are polymers that degrade fully in the sole
presence of water. In preferred embodiments, the polymers and
hydrolytic degradation byproducts are biocompatible. As used
herein, the term "non-hydrolytically degradable" refers to polymers
that do not fully degrade in the sole presence of water.
[0019] "Physiological conditions": The phrase "physiological
conditions", as used herein, relates to the range of chemical
(e.g., pH, ionic strength) and biochemical (e.g., enzyme
concentrations) conditions likely to be encountered in the
intracellular and extracellular fluids of tissues. For most
tissues, the physiological pH ranges from about 7.0 to 7.4.
[0020] "Polyelectrolyte" or "polyion": The terms "polyelectrolyte"
or "polyion", as used herein, refer to a polymer which under some
set of conditions (e.g., physiological conditions) has a net
positive or negative charge. Polycations have a net positive charge
and polyanions have a net negative charge. The net charge of a
given polyelectrolyte or polyion may depend on the surrounding
chemical conditions, e.g., on the pH.
[0021] "Polynucleotide", "nucleic acid", or "oligonucleotide": The
terms "polynucleotide", "nucleic acid", or "oligonucleotide" refer
to a polymer of nucleotides. The terms "polynucleotide", "nucleic
acid", and "oligonucleotide", may be used interchangeably.
Typically, a polynucleotide comprises at least three nucleotides.
DNAs and RNAs are polynucleotides. The polymer may include natural
nucleosides (i.e., adenosine, thymidine, guanosine, cytidine,
uridine, deoxyadenosine, deoxythymidine, deoxyguanosine, and
deoxycytidine), nucleoside analogs (e.g., 2-aminoadenosine,
2-thiothymidine, inosine, pyrrolo-pyrimidine, 3-methyl adenosine,
C5-propynylcytidine, C5-propynyluridine, C5-bromouridine,
C5-fluorouridine, C5-iodouridine, C5-methylcytidine,
7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine,
O(6)-methylguanine, and 2-thiocytidine), chemically modified bases,
biologically modified bases (e.g., methylated bases), intercalated
bases, modified sugars (e.g., 2'-fluororibose, ribose,
2'-deoxyribose, arabinose, and hexose), or modified phosphate
groups (e.g., phosphorothioates and 5'-N-phosphoramidite
linkages).
[0022] "Polypeptide", "peptide", or "protein": According to the
present invention, a "polypeptide", "peptide", or "protein"
comprises a string of at least three amino acids linked together by
peptide bonds. The terms "polypeptide", "peptide", and "protein",
may be used interchangeably. Peptide may refer to an individual
peptide or a collection of peptides. Inventive peptides preferably
contain only natural amino acids, although non-natural amino acids
(i.e., compounds that do not occur in nature but that can be
incorporated into a polypeptide chain; see, for example,
http://www.cco.caltech.edu/.about.dadgrp/Unnatstruct.gif, which
displays structures of non-natural amino acids that have been
successfully incorporated into functional ion channels) and/or
amino acid analogs as are known in the art may alternatively be
employed. Also, one or more of the amino acids in an inventive
peptide may be modified, for example, by the addition of a chemical
entity such as a carbohydrate group, a phosphate group, a farnesyl
group, an isofarnesyl group, a fatty acid group, a linker for
conjugation, functionalization, or other modification, etc. In a
preferred embodiment, the modifications of the peptide lead to a
more stable peptide (e.g., greater half-life in vivo). These
modifications may include cyclization of the peptide, the
incorporation of D-amino acids, etc. None of the modifications
should substantially interfere with the desired biological activity
of the peptide. The phrase "enzyme polypeptide" refers to a
polypeptide having enzymatic activity.
[0023] "Polysaccharide", "carbohydrate" or "oligosaccharide": The
terms "polysaccharide", "carbohydrate", or "oligosaccharide" refer
to a polymer of sugars. The terms "polysaccharide", "carbohydrate",
and "oligosaccharide", may be used interchangeably. Typically, a
polysaccharide comprises at least three sugars. The polymer may
include natural sugars (e.g., glucose, fructose, galactose,
mannose, arabinose, ribose, and xylose) and/or modified sugars
(e.g., 2'-fluororibose, 2'-deoxyribose, and hexose).
[0024] "Small molecule": As used herein, the term "small molecule"
is used to refer to molecules, whether naturally-occurring or
artificially created (e.g., via chemical synthesis), that have a
relatively low molecular weight. Typically, small molecules are
monomeric and have a molecular weight of less than about 1500
g/mol. Preferred small molecules are biologically active in that
they produce a local or systemic effect in animals, preferably
mammals, more preferably humans. In certain preferred embodiments,
the small molecule is a drug. Preferably, though not necessarily,
the drug is one that has already been deemed safe and effective for
use by the appropriate governmental agency or body. For example,
drugs for human use listed by the FDA under 21 C.F.R. .sctn..sctn.
330.5, 331 through 361, and 440 through 460; drugs for veterinary
use listed by the FDA under 21 C.F.R. .sctn..sctn. 500 through 589,
incorporated herein by reference, are all considered acceptable for
use in accordance with the present invention.
[0025] "Bioactive agents": As used herein, "bioactive agents" is
used to refer to compounds or entities that alter, inhibit,
activate, or otherwise affect biological or chemical events. For
example, bioactive agents may include, but are not limited to,
anti-AIDS substances, anti-cancer substances, antibiotics,
immunosuppressants, anti-viral substances, enzyme inhibitors,
neurotoxins, opioids, hypnotics, anti-histamines, lubricants,
tranquilizers, anti-convulsants, muscle relaxants and
anti-Parkinson substances, anti-spasmodics and muscle contractants
including channel blockers, miotics and anti-cholinergics,
anti-glaucoma compounds, anti-parasite and/or anti-protozoal
compounds, modulators of cell-extracellular matrix interactions
including cell growth inhibitors and anti-adhesion molecules,
vasodilating agents, inhibitors of DNA, RNA or protein synthesis,
anti-hypertensives, analgesics, anti-pyretics, steroidal and
non-steroidal anti-inflammatory agents, anti-angiogenic factors,
anti-secretory factors, anticoagulants and/or antithrombotic
agents, local anesthetics, ophthalmics, prostaglandins,
anti-depressants, anti-psychotic substances, anti-emetics, and
imaging agents. In certain embodiments, the bioactive agent is a
drug.
[0026] A more complete listing of bioactive agents and specific
drugs suitable for use in the present invention may be found in
"Pharmaceutical Substances: Syntheses, Patents, Applications" by
Axel Kleemann and Jurgen Engel, Thieme Medical Publishing, 1999;
the "Merck Index: An Encyclopedia of Chemicals, Drugs, and
Biologicals", Edited by Susan Budavari et al., CRC Press, 1996, and
the United States Pharmacopeia-25/National Formulary-20, published
by the United States Pharmcopeial Convention, Inc., Rockville Md.,
2001, all of which are incorporated herein by reference.
Acronyms
[0027] The following acronyms are used herein: "SPS" is
poly(styrene sulfonate), "PAA" is poly(acrylic acid), "LPEI" is
linear poly(ethylene imine), "PDAC" is poly(diallyl dimethyl
ammonium chloride), "PAH" is poly(allylamine hydrochloride), and
"PAZO" is the azobenzene functionalized polymer
poly{1-[4-(3-carboxy-4-hydroxyphenylazo)
benzensulfonamido]-1,2-ethanediyl}.
BRIEF DESCRIPTION OF THE FIGURES
[0028] FIG. 1 depicts chemical structures of certain polymers used
in accordance with the invention. Shown are the structures for
polymer 1 (Poly1), heparin sulfate, chondroitin sulfate, and
polymer 2 (Poly2). Note that Poly1 and Poly2 differ only by two
methylene units in the backbone.
[0029] FIG. 2 illustrates a scheme that may be used to construct a
film using a cationic polymer (Poly1) comprising a cationic protein
(lysozyme). Layers of a biological polyanion are deposited between
layers of Poly1 and layers of lysozyme onto a substrate such as a
slide. Thus, the film is comprised of tetralayers, each tetralayer
having the structure (Poly1/polyanion/lysozyme/polyanion.)
[0030] FIGS. 3A-D show results from characterization studies of
(Poly1/heparin/lysozyme/heparin) tetralayers. Films were
characterized by UV-Vis spectroscopy, profilometry, and instant
dissolution methods. The number of tetralayers is plotted against
the signal recorded at the point of construction. (A): UV-Vs
absorbance of films at 280 nm with signal correction at 320 nm.
(B): Profilometry measurements of films. (C): Amounts of protein
instantaneously release from films as measured by a BCA
(bicinchoninic acid) assay. (D): Overlay of the three measurement
techniques to compare curves, showing agreement of all three
methods in detecting the characteristics of film buildup. Each
signal value is taken as a percentage of the signal at 80
tetralayers.
[0031] FIGS. 4A-D show results indicating that release is affected
by temperature of release and the number of tetralayers. Time in
days is plotted against total amount of protein released in .mu.g.
(A): Replicate samples dipped with the architecture
[(Poly1/heparin/lysozyme/heparin).sub.80] were released at room
temperature and displayed a linear release curve. (B): Films with
the architecture [(Poly1/heparin/lysozyme/heparin).sub.50] were
released at 37.degree. C. and displayed a continued linear release
profile. (C): Films comprised of various numbers of tetralayers
were released in PBS. Both the amount of protein incorporated and
the time to total release are increased with increasing numbers of
tetralayers.
[0032] FIGS. 5A-C show results of characterization studies of
Poly2-containing films. (A): The number of
(poly2/heparin/lysozyme/heparin) tetralayers is plotted against the
signal recorded at that point of construction. Each signal value is
taken as a percentage of the signal at 50 tetralayers. (B):
Comparison of Poly1 and Poly2 buildup curves. The number of
(PolyX/heparin/lysozyme/heparin) tetralayers is plotted versus the
total thickness of the film as measured by profilometry. (C): When
poly2 is layered in the architecture
[(Poly2/heparin/lysozyme/heparin).sub.50] and protein is released
at 37.degree. C., release of over 34 days is achieved, showing the
tenability of this system in response to desired
characteristics.
[0033] FIG. 6 shows results of characterization studies of
chondroitin-containing films. (A): The number of
(Poly1/chondroitin/lysozyme/chondroitin) tetralayers is plotted
against the signal recorded at that point of construction. Each
signal value is taken as a percentage of the signal at 50
tetralayers. (B): Release curves of chondroitin-containing and
heparin-containing films. (C): Release curves of films comprising
various numbers of tetralayers constructed with lysozyme and
chondroitin.
[0034] FIGS. 7A-B shows results of assays to determine function of
released protein. The total amount of protein detected using the
Micro BCA Protein Kit (Piece Biotechnologies) was plotted with the
total amount of functional protein detected using the kinetic
functional lysozyme assay; both values were plotted versus
time.
[0035] FIG. 8 shows effects of dipping temperature on protein
incorporation. The dipping process was performed in a cold room at
about 4.degree. C., and an identical experiment was performed at
room temperature. Both experiments were released at room
temperature to show the effect of dipping temperature on the amount
of protein incorporated.
DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS
[0036] As mentioned above, the invention provides, in various
embodiments, systems for releasing proteins (including those having
therapeutic value) in a controlled manner, while retaining activity
of the released protein. Control may be achieved over dose, release
rate, and/or time span.
Decomposable Films
[0037] Decomposition of the thin films of the invention is
characterized by the substantially sequential degradation of at
least a portion of the polyelectrolyte layers that make up the thin
films. The degradation may be at least partially hydrolytic, at
least partially enzymatic, at least partially thermal, and/or at
least partially photolytic. In some embodiments, the thin films are
about 1 nm and about 100 .mu.m thick, for example, between about 1
nm and about 100 nm thick, between about 100 nm and about 1 .mu.m
thick, between about 1 .mu.m and about 10 .mu.m thick, or between
about 10 .mu.m and about 100 .mu.m thick.
[0038] Films are generally comprised of alternating layers of
surface erodible polyelectrolytes (such as degradable polymers) and
ionic proteins. The film may be comprised of multilayer units with
alternating layers of opposite charge, such as alternating anionic
and cationic layers. For example, a cationic polyelectrolyte may be
layered next to an anionic protein layer, and the bilayer unit
repeated to make the thin film. Alternatively, an anionic
polyelectrolyte may be layered next to a cationic protein
layer.
[0039] At least one of the layers in a multilayer unit includes a
degradable polyelectrolyte. As an example, the film may be
comprised of an at least partially degradeable polycationic layer
and a layer of anionic protein. The thin film may be exposed to a
degrading medium (e.g., intracellular fluid), whereupon the
polycationic layers degrade and the protein layers delaminate
sequentially from the surface toward the substrate. Proteins are
thus gradually and controllably released from the surface of the
thin film.
[0040] It will be appreciated that the roles of the layers of the
thin film can be reversed. In such embodiments, the polyanionic
layers include a degradable polyanion and the polycationic layers
may include, for example, a polycationic protein. Alternatively,
both the polycationic and polyanionic layers may both include
degradable polyelectrolytes.
[0041] The invention also provides thin films in which the protein
has the same charge as the chosen surface erodible polyelectrolyte.
One such embodiment of the invention is illustrated in FIG. 2, in
which a film is created by deposition of tetralayer units on a
substrate via layer-by-layer assembly. The tetralayer units
depicted in FIG. 2 comprise a layer of a cationic polymer (Poly1),
a layer of a biologically active polyanion, a layer of the protein
of interest (lysozyme in the example shown), and another layer of
biologically active polyanion. Degradation of the film and
controlled release of proteins form the surface of the thin film
proceeds as described above, except that sequential removal of the
intervening polyanionic layers also occurs.
[0042] Degradable polyelectrolytes and their degradation byproducts
may be biocompatible so as to make the films amenable to use in
vivo.
Assembly Methods
[0043] In certain embodiments, the LBL assembly of films may
involve a series of dip coating steps in which the substrate is
dipped in alternating polycationic and polyanionic solutions.
Additionally or alternatively, it will be appreciated that
deposition of alternating polycationic and polyanionic layers may
also be achieved by spray coating, brush coating, roll coating,
spin casting, or combinations of any of these techniques.
[0044] In certain embodiments, multiple layers of oppositely
charged polymers are deposited on a charged surface from aqueous
baths in a highly controllable process. Proteins can be
incorporated into individual layers of the film, affording the
opportunity for exquisite control of loading and release from the
film. There are several advantages to this technique, including
mild aqueous processing conditions (which may allow preservation of
biomolecule function); nanometer-scale conformal coating of
surfaces; and the flexibility to coat objects of any size, shape or
surface chemistry, leading to versatility in design options.
Substrates for Constructing Films
[0045] A variety of materials can be used as substrates of the
present invention such as, but not limited to, metals, e.g., gold,
silver, platinum, and aluminum; metal-coated materials; metal
oxides; plastics; ceramics; silicon; glasses; mica; graphite;
hydrogels; and polymers such as polyamides, polyphosphazenes,
polypropylfumarates, polyethers, polyacetals, polycyanoacrylates,
polyurethanes, polycarbonates, polyanhydrides, polyorthoesters,
polyhydroxyacids, polyacrylates, ethylene vinyl acetate polymers
and other cellulose acetates, polystyrenes, poly(vinyl chloride),
poly(vinyl fluoride), poly(vinyl imidazole), poly(vinyl alcohol),
poly(ethylene terephthalate), polyesters, polyureas, polypropylene,
polymethacrylate, polyethylene, poly(ethylene oxide)s and
chlorosulphonated polyolefins; and combinations thereof. For
example, a substrate of one material may be coated with a second
material, or two materials may be combined to form a composite.
[0046] It will be appreciated that materials with an inherently
charged surface are particularly attractive substrates for LBL
assembly of a thin film. Alternatively, a range of methods are
known in the art that can be used to charge the surface of a
material, including but not limited to plasma processing, corona
processing, flame processing, and chemical processing, e.g.,
etching, micro-contact printing, and chemical modification. For
example, plastics can be used as substrates, particularly if they
have been chemically modified to present polar or charged
functional groups on the surface. Additionally or alternatively,
substrates can be primed with specific polyelectrolyte bilayers
such as, but not limited to, LPEI/SPS, PDAC/SPS, PAH/SPS, LPEI/PAA,
PDAC/PAA, and PAH/PAA bilayers, that form readily on weakly charged
surfaces and occasionally on neutral surfaces. It will be
appreciated that primer layers provide a uniform surface layer for
further LBL assembly and are therefore particularly well suited to
applications that require the deposition of a uniform thin film on
a substrate that includes a range of materials on its surface,
e.g., an implant (such as stent) or a complex tissue engineering
construct.
[0047] The substrate geometry may be manipulated to deposit films
having a variety of shapes. For example, films may be deposited on
particles, tubes, or spheres to facilitate a more uniform release
distribution. Films may be deposited on strands such as sutures to
release factors such as analgesics or antibiotics at a surgical
site; coiled strands may also serve as substrates. Alternatively,
these films may be deposited onto capillary networks or tissue
engineering constructs. For example, a thin film deposited on a
three-dimensional tissue engineering construct may be used to
attract cells to a newly implanted construct and then to promote
specific metabolic or proliferative activity.
[0048] Methods of the invention may also be used to create
three-dimensional microstructures. For example, the thin film may
be deposited on a substrate that can be dissolved to leave a hollow
shell of the thin film. Alternatively or additionally, multi-layers
may be deposited on substrates having regions that are more and
less degradable. Degradation of the degradable portions leaves a
three-dimensional microstructure. In a first step, the surface of a
substrate is divided into regions in which LBL deposition of an
inventive thin film is more or less favorable. In one embodiment, a
pattern of self-assembled monolayers (SAMs) is deposited on a
substrate surface by microcontact printing (see, for example, U.S.
Pat. No. 5,512,131 to Kumar et al., see also Kumar et al., Langmuir
10:1498, 1994; Jiang and Hammond, Langmuir, 16:8501, 2000; Clark et
al., Supramolecular Science 4:141, 1997; and Hammond and
Whitesides, Macromolecules 28:7569, 1995). In some embodiments, the
substrate surface is neutral and the exposed surface of the
deposited SAMs is polar or ionic (i.e., charged). A variety of
polymers with polar or ionic head groups are known in the art of
self-assembled monolayers. In some embodiments, a uniform coating
of a polymer is deposited on a substrate, and that coating is
transformed into a patterned layer by means of photolithography.
Other embodiments are also contemplated in which the substrate
surface is selectively exposed to plasmas, various forms of
electromagnetic radiation, or to electron beams. In yet other
embodiments, the substrate may possess the desired surface
characteristics by virtue of its inherent composition. For example,
the substrate may be a composite in which different regions of the
surface have differing compositions, and thus different affinities
for the polyelectrolyte to be deposited.
[0049] In a second step, polyelectrolyte layers of alternating
charge are deposited by LBL on receptive regions of the surface as
described for a homogeneous surface above and selective regions in
Jiang and Hammond, Langmuir, 16:8501, 2000; Clark et al.,
Supramolecular Science 4:141, 1997; and Hammond and Whitesides,
Macromolecules 28:7569, 1995. The surface is subsequently flooded
with a non-degradable polymer and placed in a medium wherein at
least a portion of the polyelectrolyte layers degrade, thereby
creating a three-dimensional "tunnel-like" structure that reflects
the pattern on the original surface (see FIG. 9, step D). It will
be appreciated that more complex microstructures could be created
based on these simple principles (e.g., by depositing SAMs with
different electrostatic character in different regions of a
substrate surface and/or by iterative additions of subsequent
structures above the deposited non-degradable polymer).
Degradable Polyelectrolytes
[0050] Any degradable polyelectrolyte can be used in a thin film of
the present invention, including, but not limited to,
hydrolytically degradable, biodegradable, thermally degradable, and
photolytically degradable polyelectrolytes. Hydrolytically
degradable polymers known in the art include for example, certain
polyesters, polyanhydrides, polyorthoesters, polyphosphazenes, and
polyphosphoesters. Biodegradable polymers known in the art,
include, for example, certain polyhydroxyacids,
polypropylfumarates, polycaprolactones, polyamides, poly(amino
acids), polyacetals, polyethers, biodegradable polycyanoacrylates,
biodegradable polyurethanes and polysaccharides. For example,
specific biodegradable polymers that may be used in the present
invention include but are not limited to polylysine, poly(lactic
acid) (PLA), poly(glycolic acid) (PGA), poly(caprolactone) (PCL),
poly(lactide-co-glycolide) (PLG), poly(lactide-co-caprolactone)
(PLC), and poly(glycolide-co-caprolactone) (PGC). Those skilled in
the art will recognize that this is an exemplary, not
comprehensive, list of biodegradable polymers. The properties of
these and other polymers and methods for preparing them are further
described in the art. See, for example, U.S. Pat. Nos. 6,123,727;
5,804,178; 5,770,417; 5,736,372; 5,716,404 to Vacanti; 6,095,148;
5,837,752 to Shastri; 5,902,599 to Anseth; 5,696,175; 5,514,378;
5,512,600 to Mikos; 5,399,665 to Barrera; 5,019,379 to Domb;
5,010,167 to Ron; 4,806,621; 4,638,045 to Kohn; and 4,946,929 to
d'Amore; see also Wang et al., J. Am. Chem. Soc. 123:9480, 2001;
Lim et al., J. Am. Chem. Soc. 123:2460, 2001; Langer, Acc. Chem.
Res. 33:94, 2000; Langer, J. Control Release 62:7, 1999; and Uhrich
et al., Chem. Rev. 99:3181, 1999. Of course, co-polymers, mixtures,
and adducts of these polymers may also be employed.
[0051] The anionic polyelectrolytes may be degradable polymers with
anionic groups distributed along the polymer backbone. The anionic
groups, which may include carboxylate, sulfonate, sulphate,
phosphate, nitrate, or other negatively charged or ionizable
groupings, may be disposed upon groups pendant from the backbone or
may be incorporated in the backbone itself. The cationic
polyelectrolytes may be degradable polymers with cationic groups
distributed along the polymer backbone. The cationic groups, which
may include protonated amine, quaternary ammonium or
phosphonium-derived functions or other positively charged or
ionizable groups, may be disposed in side groups pendant from the
backbone, may be attached to the backbone directly, or can be
incorporated in the backbone itself.
[0052] For example, a range of hydrolytically degradable amine
containing polyesters bearing cationic side chains have recently
been developed (Putnam et al. Macromolecules 32:3658-3662, 1999;
Barrera et al. J. Am. Chem. Soc. 115:11010-11011, 1993; Kwon et al.
Macromolecules 22:3250-3255, 1989; Lim et al. J. Am. Chem. Soc.
121:5633-5639, 1999; Zhou et al. Macromolecules 23:3399-3406, 1990;
each of which is incorporated herein by reference). Examples of
these polyesters include poly(L-lactide-co-L-lysine) (Barrera et
al. J. Am. Chem. Soc. 115:11010-11011, 1993; incorporated herein by
reference), poly(serine ester) (Zhou et al. Macromolecules
23:3399-3406, 1990; which is incorporated herein by reference),
poly(4-hydroxy-L-proline ester) (Putnam et al. Macromolecules
32:3658-3662, 1999; Lim et al. J. Am. Chem. Soc. 121:5633-5639,
1999; each of which is incorporated herein by reference), and more
recently, poly[.alpha.-(4-aminobutyl)-L-glycolic acid].
[0053] In addition, poly(.beta.-amino ester)s, prepared from the
conjugate addition of primary or secondary amines to diacrylates,
are suitable for use with the invention. Typically,
poly(.beta.-amino ester)s have one or more tertiary amines in the
backbone of the polymer, preferably one or two per repeating
backbone unit. Alternatively, a co-polymer may be used in which one
of the components is a poly(.beta.-amino ester). Poly(.beta.-amino
ester)s are described in U.S. Ser. No. 09/969,431, filed Oct. 2,
2001, entitled "Biodegradable poly(.beta.-amino esters) and uses
thereof" and Lynn et al., J. Am. Chem. Soc. 122:10761-10768, 2000,
the entire contents of both of which are incorporated herein by
reference.
[0054] Exemplary poly(.beta.-amino ester)s include
##STR00001##
[0055] Exemplary R groups include hydrogen, branched and unbranched
alkyl, branched and unbranched alkenyl, branched and unbranched
alkynyl, aryl, halogen, hydroxyl, alkoxy, carbamoyl, carboxyl
ester, carbonyldioxyl, amide, thiohydroxyl, alkylthioether, amino,
alkylamino, dialkylamino, trialkylamino, cyano, ureido, a
substituted alkanoyl group, cyclic, cyclic aromatic, heterocyclic,
and aromatic heterocyclic groups, each of which may be substituted
with at least one substituent selected from the group consisting of
branched and unbranched alkyl, branched and unbranched alkenyl,
branched and unbranched alkynyl, amino, alkylamino, dialkylamino,
trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic,
cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide,
carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl,
alkylthioether, and thiol groups.
[0056] Exemplary linker groups A and B include carbon chains of 1
to 30 carbon atoms, heteroatom-containing carbon chains of 1 to 30
atoms, and carbon chains and heteroatom-containing carbon chains
with at least one substituent selected from the group consisting of
branched and unbranched alkyl, branched and unbranched alkenyl,
branched and unbranched alkynyl, amino, alkylamino, dialkylamino,
trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic,
cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide,
carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl,
alkylthioether, and thiol groups. The polymer may include, for
example, between 5 and 10,000 repeat units.
[0057] In some embodiments of the invention, the poly(.beta.-amino
ester) are Poly1 and/or Poly2 (whose structures are shown in FIG.
1).
[0058] Alternatively or additionally, zwitterionic polyelectrolytes
may be used. Such polyelectrolytes may have both anionic and
cationic groups incorporated into the backbone or covalently
attached to the backbone as part of a pendant group. Such polymers
may be neutrally charged at one pH, positively charged at another
pH, and negatively charged at a third pH. For example, a film may
be deposited by LBL deposition using dip coating in solutions of a
first pH at which one layer is anionic and a second layer is
cationic. If the film is put into a solution having a second
different pH, then the first layer may be rendered cationic while
the second layer is rendered anionic, thereby changing the charges
on those layers.
[0059] The composition of the polyanionic and polycationic layers
can be fine-tuned to adjust the degradation rate of each layer
within the film. For example, the degradation rate of
hydrolytically degradable polyelectrolyte layers can be decreased
by associating hydrophobic polymers such as hydrocarbons and lipids
with one or more of the layers. Alternatively, the polyelectrolyte
layers may be rendered more hydrophilic to increase their
hydrolytic degradation rate. In certain embodiments, the
degradation rate of a given layer can be adjusted by including a
mixture of polyelectrolytes that degrade at different rates or
under different conditions. In other embodiments, the polyanionic
and/or polycationic layers may include a mixture of degradable and
non-degradable polyelectrolytes. Any non-degradable polyelectrolyte
can be used with the present invention. Exemplary non-degradable
polyelectrolytes that could be used in thin films are shown in FIG.
4 and include poly(styrene sulfonate) (SPS), poly(acrylic acid)
(PAA), linear poly(ethylene imine) (LPEI), poly(diallyldimethyl
ammonium chloride) (PDAC), and poly(allylamine hydrochloride)
(PAH).
[0060] Alternatively or additionally, the degradation rate may be
fine-tuned by associating or mixing non-biodegradable, yet
biocompatible polymers (polyionic or non-polyionic) with one or
more of the polyanionic and/or polycationic layers. Suitable
non-biodegradable, yet biocompatible polymers are well known in the
art and include polystyrenes, certain polyesters, non-biodegradable
polyurethanes, polyureas, poly(ethylene vinyl acetate),
polypropylene, polymethacrylate, polyethylene, polycarbonates, and
poly(ethylene oxide)s.
Proteins
[0061] Proteins that can be incorporated into films of the present
invention include, but are not limited to, growth factors, clotting
factors, and/or enzyme polypeptides. It may be desirable, for
example, to release growth factors for tissue engineering purposes,
and/or for implantable medical devices. Growth factors that may be
released using films provided by the present invention include, but
are not limited to, vascular endothelian growth factor (VEGF), bone
morphogenic protein 2 (BMP-2), bone morphogenic protein 4 (BMP-4),
and basic fibroblast growth factor.
[0062] Release of clotting factors (also known as coagulation
factors) may promote desired biological processes such as wound
healing in certain embodiments of the invention. The clotting
factor may be, but is not limited to, factor I (fibrinogen), factor
VII (prothrombin), factor III (tissue thromboplastin), factor V
(proaccelerin), factor VII (proconvertin), factor VIII
(antihemophilic factor), factor IX (plasma thromboplastin
component), factor X (thrombokinase), factor XI (plasma
thromboplastin antecedent), factor XII (Hageman factor), and factor
XIII (fibrin stabilizing factor).
[0063] Enzymes other than some of the clotting factors listed above
may also be released using films and methods of the present
invention. For example, lysozyme lyse bacteria and may have
therapeutic uses. Lysozyme is used as a model enzyme in Examples
3-8 in the present application. Example 8 demonstrates that enzyme
released from films of the invention retain catalytic activity.
[0064] More than one protein can be released from a single film.
For example, films may be constructed so as to have multiple layers
of proteins (each layer containing different proteins), and/or such
that the "protein layer" in each multilayer unit comprises more
than one protein. This may be useful in applications, for example,
that require or would benefit from release of more than one growth
factor.
Polyions
[0065] Polyionic layers may be used in film construction and placed
between protein layers and polyelectrolyte layers having the same
charge. As discussed above, for example, in some embodiments, films
comprise tetralayer units having the structure (degradable cationic
polyelectrolyte/polyanion/cationic protein/polyanion). (Structures
with reversed charge schemes, e.g., comprising anionic
polyelectrolytes, polycations, and anionic proteins, may also be
possible with the present invention.)
[0066] Polyions used in accordance with the invention are generally
biologically derived, though they need not be. Polyions that may be
used in accordance with the invention include charged
polysaccharides. This include glycosaminoglycans such as heparin,
chondroitin, dermatan, hyaluronic acid, etc. (Some of these terms
for glycoasminoglycans are often used interchangeably with the name
of the sulfate form, e.g., heparan sulfate, chondroitin sulfate,
etc. It is intended that such sulfate forms are included among the
list of polyions that may be used in accordance with the invention.
Similarly, other derivatives or forms of such polysaccharides may
be incorporated into films.)
[0067] In some embodiments, polyions also add characteristics to
the film that are useful for medical applications. For example,
heparin activates antithrombin III, which blocks thrombin activity,
and therefore reduces clotting. Anti-clotting properties may be
desirable for a delivery device or implant coating, as clotting as
a serious concern with any device that may be put into contact with
the bloodstream (i.e., stents). Failure to address this concern may
lead myocardial infarction, stroke, or ischemia of other vital
organs.
[0068] In some applications, anticoagulation would be problematic
(for example, at a wound site) and other characteristics would be
desirable. For example, it may be desired to speed integration of
an implanted device into surrounding host tissue. Providing an
extracellular matrix-like environment that encourages cell
proliferation may facilitate integration. In such cases, a polyion
such as chondroitin (a native extracellular matrix component with
anti-inflammatory properties) could be used.
Dose and Release Characteristics
[0069] As mentioned above, certain characteristics of degradable
thin films of the invention may be modulated to achieve desired
protein doses and/or release kinetics. Doses may be modulated, for
example, by changing the number of multilayer units that make up
the film, the type of degradable polyelectrolyte used, the type of
polyion (if any) used, and/or concentrations of protein solutions
used during construction of the films. Similarly, release kinetics
(both rate of release and duration of protein release) may be
modulated by changing any or a combination of the aforementioned
factors.
Methods
[0070] Also provided in the invention are methods of releasing a
protein from a thin film of the invention. Such methods generally
comprise steps of providing a decomposable thin film of the
invention and placing the thin film in a medium in which at least a
portion of the thin film decomposes via the substantially
sequential removal of at least a portion of the layers having the
first charge and degradation of layers having the second charge.
The medium can be, for example, provided from in vivo environment
such as a subject's body. In some embodiments, the medium can be
provided in an artificial environment such as tissue engineering
scaffold. Buffers such as phosphate-buffered saline may also serve
as a suitable medium.
[0071] Release of protein may follow linear kinetics over a period
of time. Such a release profile may be desirable to effect a
particular dosing regimen. Certain embodiments of the invention
provide systems for releasing proteins in a linear fashion over a
period of at least 5, 10, or 14 days (see, for example, FIG. 4A).
Certain embodiments of the invention provide systems allowing
protein release over a period of at least 5, 10, 15, 20, 25, 30, or
34 days (see, for example, FIG. 5C).
[0072] Provided methods and systems allow release of functional
proteins, such as discussed below in Example 8. In some
embodiments, protein from thin films has at least 50%, 60%, 70%,
80%, and 90% activity (functional protein as compared to total
protein) after being released from the films. In some embodiments,
proteins released from thin films maintain up to as much 100%
activity (see, for example, FIG. 7.)
EXAMPLES
Example 1
Construction of Decomposable Protein-Releasing Films
[0073] In this Example, decomposable protein-releasing films were
constructed by layer-by-layer deposition onto glass or quartz
substrates. Biological polyanions were used between layers of
cationic polymers and layers of cationic protein, thus generating a
tetralayer structure that was repeated to build the film.
Reagents and Solutions
[0074] Linear poly(ethylenimine) (LPEI, Mn=25000) was obtained from
Polysciences, Inc (Warrington, Pa.) and poly(sodium
4-styrenesulfonate) (PSS, Mn=1000000) was obtained from
Sigma-Aldrich (St. Louis, Mo.). Chondroitin sulfate sodium salt
(Mn=60000) was obtained from VWR Scientific (Edison, N.J.) and
heparin sodium salt was obtained from Celsus Laboratories
(Cincinnati, Ohio). Poly 1 was synthesized as previously
described.sup.15. Poly 2 was synthesized in a manner similar to
that used to synthesize Poly 1. Lysozyme and Micrococcus
lysodeikticus bacteria were obtained from Sigma Aldrich (St. Louis,
Mo.). All commercial polyelectrolytes were used as received without
further purification. A Micro BCA Protein Assay Kit was obtained
from Pierce (Rockford, Ill.) and used according to manufacturer
instructions. Glass and quartz slides (substrates) were obtained
from VWR Scientific (Edison, N.J.). Deionized water (18.2 M.OMEGA.,
Milli-Q Ultrapure Water System, Millipore) was used for all washing
steps. Dulbecco's PBS buffer was prepared from 10.times.
concentrate available from Invitrogen (Frederick, Md.).
Preparation of Polyelectrolyte Solutions
[0075] LPEI and PSS were dissolved in deionized water to a
concentration of 10 mM with respect to repeat unit and pH adjusted
to 4.25 and 4.75 respectively. Heparin, chondroitin, Poly 1, and
Poly 2 were prepared in sodium acetate buffer (pH 5.1, 100 mM) at a
concentration of 2 mg/mL. Lysozyme was prepared at a concentration
of 0.5 mg/mL in 100 mM sodium acetate buffer, pH 5.1.
Film Construction
[0076] Glass substrates or quartz slides (1''.times.1/4'') were
rinsed with methanol and deionized water, dried under a stream of
dry nitrogen, and plasma-etched in oxygen using a Harrick PDC-32G
plasma cleaner on high RF power for 5 minutes. Ten base layers of
(LPEI/PSS) were deposited upon plasma-etched substrates with a Carl
Zeiss HSM series programmable slide stainer according to the
following protocol: 5 minutes of dipping in LPEI, followed by three
washes (10, 20, and 30 s each) in deionized water, followed by 5
minutes in PSS and three deionized water washes (10, 20 and 30 s
each) for 10 repetitions. On top of the base layers, tetralayers
incorporating lysozyme were built with the following architecture:
(Poly 1/heparin/lysozyme/heparin).sub.n, where n refers to the
number of tetralayers deposited on the substrate. A typical dipping
protocol would be 10 minutes in a solution of Poly 1, 3 washes (10,
20, and 30 s each), 7.5 minutes in heparin with 3 washes (10, 20,
and 30 s each), 10 minutes in the protein with 2 washes (20 and 30
s each) and 7.5 minutes in heparin with 3 washes (10, 20, and 30 s
each). For the films characterized and used in Examples 2-6, Poly1
was dissolved in 100 mM sodium acetate buffer, pH 5.1. The protein
used in the present Example (lysozyme) and the polyanion (heparin)
were similarly dissolved in 100 mM sodium acetate buffer, pH 5.1.
In the present Example, films were controlled to be approximately
0.75 inches.times.approximately 0.25 inches in size, or
approximately 1.2 cm.sup.2.
[0077] At the dipping conditions used (pH 5), Poly1 is cationic,
and lysozyme was anticipated to be cationic as well (isoelectric
point of 11). A four-layered repeat unit architecture with an
anionic polymer (see FIG. 2) in between Poly1 and lysozyme was
used, allowing deposition of layers of opposite charges adjacent to
one another. Films were thus comprised of tetralayers having the
structure (Poly1/heparin/lysozyme/heparin).
Example 2
Characterization of Protein-Releasing Films
[0078] In this Example, protein-releasing films constructed as
described in Example 1 were characterized in terms of protein
incorporation and growth.
Materials and Methods
[0079] Varying numbers of tetralayers were deposited onto clean
quartz substrates pre-treated with 10 base layers. Three techniques
were used to analyze buildup. The thickness of the resulting films
was measured by scoring the samples to the base of the film with a
razor blade and measuring the step height using a profilometer (P10
Surface Profiler) with a 2 .mu.m tip radius stylus. Protein
incorporation in the film was measured by UV-Vis spectroscopy. A
profile was taken of each sample on a Cary 6000i spectrophotometer
from 200-800 nm. Proteins absorb at 280 nm due to tryptophan,
tyrosine, and cysteine, and the intensity of absorption can then be
correlated to protein buildup within the film. Absorbance values at
320 nm were measured as a baseline value and was subtracted from
absorbance values at 280 nm. Baseline-corrected absorbance values
were plotted against number of tetralayers.
[0080] To quantify the total protein concentration in the film,
constructed films were spontaneously dissolved using 1 mL of 1M
NaOH for 1 hour, which disrupts film architecture and releases all
incorporated drug. A 50 .mu.L sample was quenched in 1.times.PBS
and read using a Micro BCA Protein Assay Kit (Pierce Biotechnology,
Rockford, Ill.). Bicinchoninic acid can be used to quantify protein
concentration by detecting a reduction of copper by the protein of
interest in an alkaline environment. A color change can be
monitored and compared to a constructed standard curve. Triplicate
100 .mu.L aliquots of standards and samples were run in 96 well
plates according to the manufacturer's protocol and read on a
microplate reader (PowerWave XS, BioTek, Winooski VT).
[0081] Using measurements from standard samples of known
concentration, a standard curve of concentration versus slope was
constructed for the concentration range 0-200 .mu.g/mL. The
standard curve was linear over this concentration range. Lysozyme
concentrations of unknown samples were interpolated from the
standard curve using the slopes for each sample. The error bars in
FIGS. 3-7 represent a 99% confidence interval calculated from
triplicate repeat samples of 2 independent trials (resulting in a
total of 6 data points for each sample or standard.
Results and Discussion
[0082] Film buildup was tracked by monitoring thickness, protein
incorporation, and instantaneous protein release (see FIG. 3).
After a brief induction period where little protein incorporation,
film thickness, or protein release is achieved (approximately 10-20
tetralayers), all three methods indicate that the films build and
incorporate protein in a roughly linear fashion (FIG. 3 A-D).
[0083] An induction period for multilayer growth is typical of many
LbL systems and has been reported in the literature.sup.23,24. It
is believed, without being held to theory, that in this initial
period, surface effects influence the buildup of the LbL film until
complete surface coverage is achieved after several adsorption
cycles. Furthermore, superlinear growth occurs as interdiffusion
occurs within the layers of the film.
[0084] Protein content was measured by two techniques (UV-Vis, FIG.
3A; BCA, FIG. 3C), and total film buildup was measured by
profilometry as described above (see FIG. 3B). Protein
incorporation became linear after approximately 10 tetralayers,
whereas thickness increase became linear at approximately 20 to 30
tetralayers. The thickness per tetralayer repeat unit in the linear
growth regime is approximately 0.42 .mu.m (420 nm), which is large
in comparison with typical electrostatic multilayer systems that
exhibit 10 to 100 nm per bilayer pair.
[0085] The disparity between the kinetics of protein incorporation
and that of thickness suggests that although protein incorporation
is substantially linear after just 10 tetralayers, the polymer
composition in the film may be changing in a nonlinear fashion
during this initial period. This regime of superlinear growth is
hypothesized to be due to intermolecular interdiffusion of
macromolecules into the film during the adsorption process, which
leads to increasingly thick films.sup.24,25 until the linear growth
phase is reached.
[0086] It is believed, without being held to theory, that the
linear characteristics of the second growth phase is created by a
"front" in which interdiffusion of polymers into the bulk film
remains possible. Such a front may vastly increase the amount of
drug that can be incorporated in each dip step compared to the
amount of drug that can be incorporated during initial steps (when
the film and therefore the front are thinner). It is believed,
without being held to theory, that underneath the front is a
"reorganized" layer that is impermeable to diffusion, retaining the
linearity of the film.sup.23-25. The diffusive character of the
front and therefore of the film are affected by a number of factors
including hydrophilicity of the polymer backbone, charge density of
the polyions, and molecular weight of the polymers involved.
[0087] In general, there is strong agreement between the three
measurement techniques, indicating excellent protein incorporation
in the films, with linear build-up after an initial induction
period (see FIG. 3D). This linearity makes it simple to predict the
additional amount of protein incorporated by adding subsequent
tetralayers, as the amount incorporated increases linearly with the
additional number of tetralayers dipped. For
[Poly1/heparin/lysozyme/heparin].sub.80 films, a UV-Vis signal of
2.064 units at 280 nm (FIG. 1A) corresponded with a profilometry
thickness of 23.7 .mu.m (23780 nm) (FIG. 1B), or approximately 1365
.mu.g of loaded lysozyme.
Example 3
Characterization of Protein Release from Films
[0088] In this Example, total protein released from films was
determined as a function of time.
Materials and Methods
[0089] Samples were released into phosphate buffered saline (pH
7.4) at either room temperature or 37 degrees Celsius in a
microcentrifuge tube containing 1 mL of PBS. At a series of
different time points, 0.5 mL of sample was removed and 0.5 mL of
fresh PBS was introduced to the sample container. Samples were
frozen at -20.degree. C. until analyzed. Release of lysozyme into
the solution was detected using the Micro BCA Protein Assay Kit as
described in Example 2. For each time point analyzed, the total
amount of protein released up to the time point was measured.
Results and Discussion
[0090] Proteins were released from films at 37.degree. C. in PBS to
approximate physiologic conditions and analyzed using a Micro BCA
kit. As shown in FIG. 4A (which depicts a release curve for 43
tetralayer-films), lysozyme is released with a linear trend over a
period of approximately 14 days. Approximately 780 .mu.g of
incorporated protein (or 650 .mu.g/cm.sup.2) was released over the
approximately 14-day time period.
[0091] The linear release trend may be desirable from multiple
perspectives. This release trend may desirable for the drug
delivery applications, because it allows constant, low levels of
protein to be released from the surface. In burst release profiles,
most of the protein is released instantly, consequently lost to a
greater body volume, and cleared before therapeutic action can take
place. In such burst release profiles, only a minority of drug is
controlled in release. In contrast with burst release profiles,
nearly all of the release from protein-releasing films of the
invention occurs in a controlled, easy to predict (and therefore
dose-oriented) fashion based on numbers of tetralayers used to
build the film. Controlled delivery has significant advantages over
pill or bolus injection methods, as it allows the concentration of
the protein at the local site of interest to be kept within a
therapeutic window between an upper limit of toxicity and a lower
limit of effectiveness. In some embodiments, the invention
therefore provides a new modality for the timed local release of
proteins within the body where the expense or size of a dose was
previously prohibitive. Loading more drug is possible by increasing
the number of tetralayers in the film; it is also possible to
predict the additional amount of protein incorporated.
[0092] In addition to these advantages, the controlled and linear
release profile suggests a surface erosion mechanism for release.
Previous studies of the degradation of
poly(.beta.-aminoester)-based LbL delivery indicated that
degradation indeed occurs by surface-based erosion, based on AFM
and similar measurements. The experiments described in this Example
also illustrate that very large amounts of protein can be
incorporated even in a mid-range number of tetralayers (films from
10 to 80 tetralayers were examined). Protein incorporation of up to
approximately 1365 .mu.g (or 1.14 mg/cm.sup.2) is possible at 80
tetralayers in this particular Example (see FIG. 3C). Most growth
factor applications, by comparison, require only nanogram/mL
concentrations of protein, suggesting that these films have a high
upper limit loading capacity for such applications.
Example 4
Tuning of Total Dose and Time Scale of Release by Modifying the
Number of Multilayer Units
[0093] It can be envisioned that by tuning the number of
tetralayers used in this system, it would be possible to change
both the total dose administered as well as the time scale of
release. FIG. 4B depicts a family of release curves for films with
(Poly 1/heparin/lysozyme/heparin), architecture and varying numbers
of tetralayers. As can be observed from FIG. 4B, with increasing
numbers of tetralayers, both the total amount of drug released and
the time span of release are increased, consistent with the
hypothesized mechanism of surface erosion. Films with 20
tetralayers released 100 .mu.g of protein over 7 days, while films
with 43 tetralayers released 780 .mu.g of protein over 14 days.
Bulk releasing films would possibly have increased loading with
increasing numbers of tetralayers, but would release the entire
load over the same amount of time, independent of the number of
tetralayers. This characteristic of bulk releasing films, when
taken together with the linear buildup and release of the film,
supports the idea that a surface erosion mechanism of release
(rather than a bulk diffusion mechanism) is responsible, as
demonstrated and discussed in previous publications.sup.2,19.
[0094] Release studies also allowed for the calculation of the
fraction of loaded films that is actually released. To address this
question, two sets of films were constructed with the architecture
(Poly1/heparin/lysozyme/heparin) and varying numbers of
tetralayers. One set of films was released at 37.degree. C. and the
other was instantaneously released using a rapid deconstruction of
the film, as described in the Materials and Methods section of
Example 3. The total release was calculated in each instance, and
plotted in FIG. 4C. Nearly 95% release or higher is observed at low
numbers of tetralayers (50 tetralayers and below). At 80
tetralayers, approximately 85% release is achieved. Because this is
a fully degradable system, it is anticipated that eventual recovery
of the remaining 15% would occur over more extended time periods.
Without being held to theory, it is hypothesized that the thickness
of the film and incomplete degradation of overlying polymer
networks hinders further release of protein from the film.
Example 5
Tuning of Total Dose and Time Scale of Release by Modifying the
Molecular Structure of the Polymer
[0095] One of advantage of using a synthetic erodible polymer is
that the drug delivery from the device can be tuned through
additional mechanisms over those already discussed by modifying the
molecular structure of the polymer used. Poly1 is only one of a
large family of poly(.beta.-aminoesters) that can be used in these
films; by tuning the composition of the polymers used for this
purpose, one can alter the degradability of the ester bond and
therefore decrease or increase the time scale over which the film
degrades.
[0096] A second poly(.beta.-aminoester), Poly2, was used to explore
the effect of the kinetics of ester hydrolysis on drug release from
the constructed multilayers (see FIG. 1). Poly2 differs from Poly1
in that it has an additional two methylene units in the backbone
next to the ester bond, making Poly2 more hydrophobic and making
the ester bond less susceptible to hydrolysis. This decreased
susceptibility to hydrolysis is predicted to decrease degradation
and therefore increase time span of release. Thus, characterization
and comparison between Poly1 and Poly2 films yields further
interesting information on the erosion of these films as well as
demonstrating that one can to tune release by tuning the
characteristics of the synthetic polymer used.
[0097] Comparing buildup data of Poly2 compared to similar films
for Poly1 (FIG. 5A), there is similar agreement between the three
measurement techniques, suggesting that linear film buildup and
incorporation are first observed at 20 tetralayers. As an
illustrative example, the profilometry data for P1 and P2 films
have been plotted in FIG. 5B to show the relative film buildup
between the two compositions (100% in FIG. 5A corresponds 50
tetralayer values: a UV-Vis reading of 1.52 absorbance units at 280
nm, a film thickness of approximately 13.23 .mu.m, and protein
incorporation of approximately 960 .mu.g). In FIG. 5B, a
representative comparison of profilometry measurements show that
while both films exhibit similar growth trends, Poly2 incorporates
more material at each measurement point. For example, at 50
tetralayers, Poly1 is able to incorporate approximately 780 .mu.g
of protein, whereas Poly2 is able to incorporate about 960 .mu.g of
protein.
[0098] The phenomenon of increasing drug loading with increasing
hydrophobicity is well understood. It is believed, without being
held to theory, that increasing hydrophobicity leads to a more
"loopy" film architecture in which there are longer segments of
polymer between electrostatic connections with the growing film and
new polymer chains being incorporated. This increases both the film
thickness and its ability to load more drug (by having a greater
volume in which to pack drug). Hydrophobic interactions between the
drug and the polymer may further enhance drug loading in these
systems.
[0099] FIG. 5C depicts amount of protein released over time from a
film with the architecture (Poly2/heparin/lysozyme/heparin).sub.n.
Over 34 days of release are observed with Poly2 films, indicating a
2.times. increase in release time using the more hydrophobic
polymer. These results demonstrate that the time period of release
can be modified by tuning the degradable polymer used. This data
further supports the idea that surface erosion is the likely
mechanism of release, as it is the hampered erosion of the ester
bond that leads to increased release times.
Example 6
Tuning of Total Dose and Time Scale of Release by Using a Different
Polyanion
[0100] Release behavior of the films can also be modified by
incorporating a different polyanion, such as chondroitin.
Chondroitin sulfate was incorporated in films of the architecture
[Poly1/chondroitin/lysozyme/chondroitin] and varying numbers of
tetralayers. Profilometry, UV-vis and instantaneous release curves
are plotted and depicted in FIG. 6A. Contrary to the film growth
behavior for heparin, chondroitin buildup is characterized by a
more extended period of time in the first, superlinear regime of
the buildup process enabled by interdiffusion. Even by 50
tetralayers, it is unclear whether the films are beginning to build
linearly or not; it is perhaps at this point that the films are
just entering the second, linear regime of interdiffusion-based
multilayer assembly. Film thickness values at 50 tetralayers are
much lower than those of Poly 1/heparin or Poly 2/heparin films,
suggesting that interdiffusion in these films may not be as
extensive as that in films built with heparin. Without being held
to theory, less extensive interdiffusion may explain the more
extended period before entering the linear
interdiffusion/permeation controlled regime observed for
chondroitin-containing films as compared to heparin-containing
films. Protein incorporation is also lower than that of comparable
heparin-based films. Table 1 summarizes UV-Vis absorbance at 280 nm
(indicative of protein incorporated), thickness, and instantaneous
protein release values for varying film constructions.
TABLE-US-00001 TABLE 1 Protein incorporation and thickness for
films of varying constructions Protein UV-Vis Thickness
instantaneously Film construction absorbance (.mu.m) released
(.mu.g) (Poly1/chondroitin/ 0.24 2.73 160
lysozyme/chondroitin).sub.50 (Poly1/heparin/lysozyme/ 1.24 7.90 780
heparin).sub.50 (Poly2/heparin/lysozyme/ 1.52 13.23 960
heparin).sub.50
[0101] Whereas linear incorporation was observed for
heparin-containing films, superlinear incorporation was observed
for chondroitin-containing films. Chondroitin-containing films
showed an inverse exponential pattern of release, exhibiting a
power law dependence with more lysozyme released first from the
lysozyme-rich top layers of the film (see FIG. 6B). The lower
incorporation of lysozyme and faster time to completion of release
of 3 to 4 days (see FIGS. 6B and 6C) are consistent with the
overall thinner film and lower loading of protein. These films may
be useful in applications in which a fairly sustainable burst at
the beginning is desirable, with low levels of sustained release
following. Shown in FIG. 6C are release curves for
chondroitin-containing films comprised of varying numbers of
tetralayers. With increasing numbers of tetralayers, a trend of
increased loading and time to complete release was observed, as had
been seen with Poly1/heparin and Poly2/heparin films. The results
in FIG. 6B also show that total dose and release kinetics of
chondroitin-containing films can be tuned.
Example 7
Activity of Protein Released from Films
[0102] One concern in encapsulating proteins for drug delivery is
whether the processing conditions will destroy the activity of the
encapsulated component. To quantify the functionality of released
enzyme, activity assays of lysozyme were performed. Lysozyme's
native activity is to cleave bacterial cell walls; one can detect
the amount of functional lysozyme in solution by a kinetic
reduction in turbidity of a bacterial solution.
Materials and Methods
[0103] Lysing of bacteria, such as can be achieved by lysozyme,
will clear a cloudy suspension of bacteria; this clearing can be
measured as a decrease in absorbance at 450 nm. Measurement was
done as a kinetic plate reading assay. When absorbance at 450 nm is
plotted against time, the slope of the graph is proportional to the
concentration of lysozyme present in the solution.
[0104] Using measurements from standard samples of known
concentration, a standard curve of concentration versus slope was
constructed for the concentration range 0-200 .mu.g/mL. The
standard curve was linear over this concentration range. Functional
lysozyme concentrations of unknown samples were interpolated from
the standard curve using the slopes for each sample. In these
tests, 290 .mu.L of a 0.25 mg/mL solution of Micrococcus
lysodeikticus was mixed with 10 .mu.L of sample or standard. Each
sample or standard was prepared in triplicate. Samples and
standards were read in a 96 well plate at 450 nm every 15 seconds
for a total of 10 readings. The readout from the assay is a
concentration of lysozyme present based on the lysing ability of
the sample, and thus represents the concentration of functional
protein present in the sample.
Results and Discussion
[0105] In FIG. 7, results of the micro-BCA assay for typical films
are plotted in tandem with the results of a functional assay that
reports the concentration of active enzyme present in a given
sample. 80%-100% of activity is preserved within the films
throughout the length of the trial. These rates of activity compare
favorably with those of protein release from LbL films in the
literature. In films described by Derbal et al..sup.26, activity at
physiologic pH was possible, but long term activity of the enzyme
(over a period of months) dropped to levels of approximately 30% of
their original value. In capsules described by Caruso et al. and
Tiourina et al..sup.9,10, excellent retention of activity was
possible, ranging from 70 to 100%; however, the capsules required a
high pH to release, which is unattainable for many medical device
release applications, and the system exhibited a burst style of
release.
[0106] By combining hydrolytic degradability in a polyion directly
with the protein of choice in LbL assembly, it is possible to
protect and retain protein for long periods of time while
sustaining the ability for extended release at biologically
relevant conditions.
Example 8
Effect of Dipping Temperature on Incorporated Protein
[0107] Due to the fragile nature of many of the proteins that could
potentially be used as drugs with films and methods of the present
invention, the possibility of dipping at a reduced temperature in
order to enhance protein stability during incorporation was
explored. To lower the temperature of the dipping baths during the
dipping process, the dipping apparatus was placed at 4.degree. C.
and the experiment run in a similar fashion to that of room
temperature. As can be seen from FIG. 8, the amount of incorporated
protein decreases with decreased with dipping temperature but
retains the general trends and characteristics during release of
films dipped at room temperature.
[0108] Although lower amounts of protein are incorporated when
dipping was performed at lower temperatures, lower dipping
temperatures may be desirable in some embodiments, particularly
with heat-sensitive proteins. Higher amounts of protein may be
achieved with lower dipping temperatures by, for example,
increasing the number of multilayers in the film and/or the
concentration of protein in the adsorption baths.
[0109] All literature and similar material cited in this
application, including, patents, patent applications, articles,
books, treatises, dissertations and web pages, regardless of the
format of such literature and similar materials, are expressly
incorporated by reference in their entirety. In the event that one
or more of the incorporated literature and similar materials
differs from or contradicts this application, including defined
terms, term usage, described techniques, or the like, this
application controls.
[0110] The section headings used herein are for organizational
purposes only and are not to be construed as limiting the subject
matter described in any way.
Other Embodiments and Equivalents
[0111] While the present inventions have been described in
conjunction with various embodiments and examples, it is not
intended that the present inventions be limited to such embodiments
or examples. On the contrary, the present inventions encompass
various alternatives, modifications, and equivalents, as will be
appreciated by those of skill in the art. Accordingly, the
descriptions, methods and diagrams of should not be read as limited
to the described order of elements unless stated to that
effect.
[0112] Although this disclosure has described and illustrated
certain embodiments of the invention, it is to be understood that
the invention is not restricted to those particular embodiments.
Rather, the invention includes all embodiments that are functional
and/or equivalents of the specific embodiments and features that
have been described and illustrated.
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