U.S. patent application number 11/782160 was filed with the patent office on 2008-01-24 for lipolysis stimulator.
This patent application is currently assigned to Kao Corporation. Invention is credited to Hiroshi Kusuoku, Shinobu Mori, Mayumi Sato.
Application Number | 20080021418 11/782160 |
Document ID | / |
Family ID | 33543566 |
Filed Date | 2008-01-24 |
United States Patent
Application |
20080021418 |
Kind Code |
A1 |
Mori; Shinobu ; et
al. |
January 24, 2008 |
LIPOLYSIS STIMULATOR
Abstract
The present invention is directed to a lipolysis stimulator and
a slimming agent which stimulate or facilitate lipolysis of
accumulated adipose tissue, to thereby exert the body slimming
effect. The lipolysis stimulator or the slimming agent of the
present invention contains as an active ingredient any form of a
plant or an extract thereof, the plant being selected from among
common juniper, togenashi, rosehip, areca, polygala root, plantago
herb, calumba, zuikorodoku, garden nasturtium,
kidachiumanosuzukusa, bayberry, cogon grass, kohon, shoyokanzo,
Japanese white birch, tanjin, kikubafuro, white mustard, common
sunflower, ground ivy, Chinese wolfberry, Japanese pagota tree,
sennenken, common fig, kankatto, Chinese hibiscus, usubaakaza,
fenugreek, English walnut, sozuku, koniwa-zakura, gardenia,
shima-kan-giku, akamino-akane, futaba-mugura, karoou, schizonepeta
spike, purslane, karabyakushi, and prostrate knotweed.
Inventors: |
Mori; Shinobu; (Haga-gun,
JP) ; Kusuoku; Hiroshi; (Haga-gun, JP) ; Sato;
Mayumi; (Tokyo, JP) |
Correspondence
Address: |
OBLON, SPIVAK, MCCLELLAND MAIER & NEUSTADT, P.C.
1940 DUKE STREET
ALEXANDRIA
VA
22314
US
|
Assignee: |
Kao Corporation
Tokyo
JP
|
Family ID: |
33543566 |
Appl. No.: |
11/782160 |
Filed: |
July 24, 2007 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
10900102 |
Jul 28, 2004 |
7300675 |
|
|
11782160 |
Jul 24, 2007 |
|
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Current U.S.
Class: |
604/290 |
Current CPC
Class: |
A61K 36/704 20130101;
A61K 36/899 20130101; A61P 3/04 20180101; A61K 36/52 20130101; A61K
36/744 20130101; A61K 36/60 20130101; A61K 36/25 20130101; A61K
36/28 20130101; A61K 36/68 20130101; A23L 33/105 20160801; A61K
36/48 20130101; A61K 8/9789 20170801; A61K 36/31 20130101; A61K
36/69 20130101; A23V 2002/00 20130101; A61P 3/06 20180101; Y10S
514/909 20130101; A61K 36/14 20130101; A61K 36/738 20130101; A61K
8/9794 20170801; A61K 36/185 20130101; A61K 36/815 20130101; A61Q
19/06 20130101; A61K 8/9761 20170801; A23V 2002/00 20130101; A23V
2200/332 20130101; A23V 2250/21 20130101 |
Class at
Publication: |
604/290 |
International
Class: |
A61M 35/00 20060101
A61M035/00 |
Foreign Application Data
Date |
Code |
Application Number |
Jul 29, 2003 |
JP |
2003-203095 |
May 26, 2004 |
JP |
2004-156535 |
Claims
1-4. (canceled)
5. A slimming method comprising applying a lipolysis stimulator
comprising as an active ingredient an aqueous or aqueous ethanol
plant extract, the plant being at least one selected from the group
consisting of togenashi, rosehip, areca, plantago herb, calumba,
zuikorodoku, garden nasturtium, kidachiumanosuzukusa, bayberry,
kohon, Japanese white birch, kikubafuro, ground ivy, Japanese
pagota tree, sennenken, common fig, kankatto, Chinese hibiscus,
usubaakaza, English walnut, sozuku, koniwa-zakura, shima-kan-giku,
akamino-akane, futaba-mugura, karoou, schizonepeta spike, purslane,
karabyakushi, and prostrate knotweed to the skin of a subject.
6. (canceled)
7. The slimming method of claim 5, wherein said lipolysis
stimulator comprises as an active ingredient 0.005 weight (wt.) %
or more, calculated on a dry basis of said aqueous or aqueous
ethanol plant extract based on the entirety of said
composition.
8. The slimming method of claim 5, wherein said lipolysis
stimulator comprises as an active ingredient 0.01 to 30 wt. %,
calculated on a dry basis of said aqueous or aqueous ethanol plant
extract based on the entirety of said composition.
9. The slimming method of claim 5, wherein said lipolysis
stimulator comprises as an active ingredient 0.02 to 25 wt. %,
calculated on a dry basis of said aqueous or aqueous ethanol plant
extract based on the entirety of said composition.
10. The slimming method of claim 5, wherein said lipolysis
stimulator comprises as an active ingredient 2 to 20 wt. %,
calculated on a dry basis of said aqueous or aqueous ethanol plant
extract based on the entirety of said composition.
Description
FIELD OF THE INVENTION
[0001] The present invention relates to a lipolysis stimulator and
a slimming agent.
BACKGROUND OF THE INVENTION
[0002] Obesity arises as a result of accumulation of neutral fat in
white adipocytes, due to excessive energy intake over energy
expenditure. As has been pointed out, a type of obesity
accompanying significant accumulation of visceral fat has some
relation with certain pathological conditions such as insulin
resistance and arteriosclerosis, and another type of obesity
accompanying significant accumulation of subcutaneous fat has
become a concern to both men and women from the aesthetic point of
view.
[0003] Hitherto, it has been widely accepted that obesity can be
well suppressed, prevented, or reversed by habitual drinking of
oolong tea or eucommia leaf tea. Moreover, for suppressing calorie
intake, a variety of means have been implemented; e.g., limiting
meals, consuming low-energy foods, and taking appetite suppressors
or digestion/absorption suppressors. However, habitual drinking of
oolong tea or eucommia leaf tea, or suppression of calorie intake,
is not necessarily sufficient for preventing or reversing obesity.
In addition, such means is difficult for people to accept as a
habit. Furthermore, these means are not a radical solution, as they
do not stimulate lipolysis of accumulated fat.
[0004] Incidentally, noradrenaline, adrenaline, and similar
hormones in the body have conventionally been known as stimulating
lipolysis. For example, compounds such as caffeine and theophylline
have been reported to stimulate the lipolysis activity of the
hormone (see, for example, Japanese Patent Application Laid-Open
(kokai) No. 53-59038). However, long-term administration of such
hormones for slimming purposes should be avoided, from the
viewpoint of safety.
SUMMARY OF THE INVENTION
[0005] The present invention provides a lipolysis stimulator and a
slimming agent, containing as an active ingredient any form of a
plant or an extract thereof, the plant being selected from among
common juniper, togenashi, rosehip, areca, polygala root, plantago
herb, calumba, zuikorodoku, garden nasturtium,
kidachiumanosuzukusa, bayberry, cogon grass, kohon, shoyokanzo,
Japanese white birch, tanjin, kikubafuro, white mustard, common
sunflower, ground ivy, Chinese wolfberry, Japanese pagota tree,
sennenken, common fig, kankatto, Chinese hibiscus, usubaakaza,
fenugreek, English walnut, sozuku, koniwa-zakura, gardenia,
shima-kan-giku, akamino-akane, futaba-mugura, karoou, schizonepeta
spike, purslane, karabyakushi, and prostrate knotweed.
[0006] The present invention also provides a slimming method
including applying the plant or an extract thereof to the skin.
[0007] The present invention also provides use of the plant or an
extract thereof in manufacture of a lipolysis stimulator or a
slimming agent.
DETAILED DESCRIPTION OF THE INVENTION
[0008] The present invention contemplates provision of a lipolysis
stimulator and a slimming agent which stimulate lipolysis of
accumulated adipose tissue, in particular, subcutaneous fat, to
thereby exert slimming effect with high safety. The present
inventors searched for a natural substance which stimulates
lipolysis and is safe to human body, and found that a certain plant
(such as common juniper) or its extract stimulates lipolysis of
neutral fat accumulated in adipose tissue, and is therefore useful
as a drug, food, or a cosmetic composition which is capable of
providing a slimming effect through suppression, prevention, or
reversal of obesity.
[0009] With the lipolysis stimulator or the slimming agent of the
present invention, slimming of the body can be realized through
suppression, prevention, or reversal of obesity.
[0010] In the plants of the present invention, common juniper
refers, in its narrow sense, to Juniperus communis which belongs to
family Cupressaceae. However, analogous plants belonging to family
Juniperus may also be used in the present invention. Similarly,
togenashi refers to Rosa normalis belonging to family Rosaceae;
rosehip refers to Rosa canina belonging to family Rosaceae; areca
refers to Areca catechu belonging to family Palmae; polygala root
refers to Polygala tenuifolia belonging to family Polygalaceael;
plantago herb refers to Plantago asiatica belonging to family
Plantaginaceae; calumba refers to Jaterorhiza columba belonging to
family Menispermaceae; zuikorodoku refers to Stellera chamaejasme
L. belonging to family Thymelaeaceae; garden nasturtium refers to
Trollius chinensis Bge. belonging to family Ranunculaceae;
kidachiumanosuzukusa refers to Aristolochia manshuriensis Kom.
belonging to family Aristolochiales; bayberry refers to Myrica
rubra Sieb. et Zucc. belonging to family Myricaceae; cogon grass
refers to Imperata cylindrica belonging to family Imperata
cylindrica (L.) P. Beauvois var. koenigii (Retz.) Durand et Schinz.
(I. cylindrica), kohon refers to Ligusticum sinense Oliv. belonging
to family Apiaceae (Umbelliferae); shoyokanzo refers to
Hemerocallis plicata Stapf belonging to family Liliaceae, Japanese
white birch refers to Betula platyphylla Suk. Var. japonica (Sieb.)
Hara belonging to family Betulaceae; tanjin refers to Saivia
miltiorrhiza Bge. belonging to family Lamiacea (Labiatae);
kikubafuro refers to Erodium stephanianum Willd. belonging to
family Geraniaceae; white mustard refers to Brassica hirta Moench
(=B. alba (L.) Boiss.) belonging to family Brassicaceae
(Crusiferae); common sunflower refers to Helianthus annuus L.
belonging to family Asteraceae; ground ivy refers to Glechoma
hederacea L. var. grandis (A. Gary) Kudo (Glechoma hederacea L.)
belonging to family Lamiaceae; Chinese wolfberry refers to Lycium
chinense Mill., L, barbarum L. belonging to family Solanaceae;
Japabese pagota tree refers to Sophora japonica L. belonging to
family Fabaceae (Leguminosae); sennenken refers to Homalornena
occulta (Lour.) Schott belonging to family Araceae; common fig
refers to Ficus carie L. belonging to family Moraceae; kankatto
refers to Pueraria thomsonii Benth. belonging to family Fabaceae
(Leguminosae); Chinese hibiscus refers to Hibiscus rosa-sinensis L.
belonging to familv Malvaceae; usubaakaza refers to Chenopodiumn
hybridum L. belonging to family Chenopodiaceae; fenugreek refers to
Trigonella foenungraecum L. belonging to family Fabaceae
(Leguminosae); English walnut refers to Juglans regia L. belonging
to family Juglandaceae; sozuku refers to Alpinia katsumadai Hayata
belonging to family Zingiberaceae; koniwa-zakura refers to Prunus
humilis Bge. P. japonica Thunb., P. tomentosa thunb. belonging to
family Rosaceae; gardenia refers to Gardenia jasminoides Ellis, G.
jasminoides Ellis var. grandiflora Nakai belonging to family
Chenopodiaceae; shima-kan-giku refers to Chrysanthemum indcum L.
belonging to family Asteraceae; akamino-akane refers to Rubia
cordifolia L. belonging to family Chenopodiaceae; futaba-mugura
refers to Hedyotis diffusa Willd. (=Oldenlandia diffusa (Willd.)
Roxb. belonging to family Chenopodiaceae; karoou refers to
Lysimachia christinae Hance belonging to family Primulaceae;
schizonepeta spike refers to Schizonepeta tenuifolia Briq.
belonging to family Lamiaceae (Labiatae); purslane refers to
Portulaca oleracea L. belonging to family purslane (Portulaca
oleracea); karabyakushi refers to Angelica dahurica Benth. et Hook.
var. pai-chi Kimura, Hata et Yen belonging to family Apiaceae
(Umbelliferae); and prostrate knotweed refers to Polygonum
aviculare L. belonging to family Polygonaceae. Analogous plants
belonging to the genera of the above-listed plants may also be
employed for the purposes of the present invention.
[0011] In the present invention, any part of the above-listed
plants may be employed as appropriate. For example, the whole
plant, leaves, bark, branches, fruits, or roots may be used in
their original forms or after processing (milled or pulverized).
Preferred portions of plants are as follows: fruit of common
juniper; fruit of togenashi; fruit of rosehip; the whole plant of
plantago herb; seeds of areca; roots of polygala root; roots of
calumba; roots of zuikorodoku; flowers of garden nasturtium; stems
of kidachiumanosuzukusa; barks of bayberry; rhizomes of cogon
grass; rhizomes of kohon; flowers of shoyokanzo; barks of Japanese
white birch; roots of tanjin; the whole plant of kikubafuro; seeds
of white mustard; seeds of common sunflower; the whole plant of
ground ivy; fruit of Vhinese wolfberry; flowers of Japanese pagota
tree; rhizomes of sennenken; fruit of common fig; roots of
kankatto; flowers of Chinese hibiscus; the whole plant of
usubaakaza; seeds of fenugreek; seeds of English walnut; seeds of
sozuku; seeds of koniwa-zakura; fruit of gardenia; flowers of
shima-kan-giku; roots of akamino-akane; the whole plant of
futaba-mugura; the whole plant of karoou; the whole plant of
schizonepeta spike; the whole plant of purslane; roots of
karabyakushi; and the whole plant of prostrate knotweed.
[0012] In the context of the present invention, the word "extract"
of any of the above-mentioned plants refers to an extract obtained
by subjecting any part of the plant to an extraction procedure at
ambient temperature or under heat with an appropriate solvent
without use of any special extraction device or through use of a
specific extractor such as a Soxhlet's extractor; a diluted
solution of an extract; a concentrate of an extract; or a dry
powder of an extract.
[0013] The extraction solvent to be used for obtaining the plant
extract of the present invention may be either polar or non-polar
solvent. A mixture of a polar and a non-polar solvent may also be
employed. Examples of the solvent include water; alcohols such as
methanol, ethanol, propanol, and butanol; polyhydric alcohols such
as propylene glycol and butylene glycol; ketones such as acetone
and methyl ethyl ketone; esters such as methyl acetate and ethyl
acetate; linear or cyclic ethers such as tetrahydrofuran and
diethyl ether; polyethers such as polyethylene glycol; hydrocarbons
such as hexane, cyclohexane, and petroleum ether; aromatic
hydrocarbons such as benzene and toluene; pyridines; super critical
carbon dioxide; fats and oils; waxes; and other types of oils. Of
these materials, water, alcohols, and water-alcohol mixtures are
preferred, with water-ethanol mixtures, inter alia, a water-ethanol
mixture which contains 20 to 80% (vol/vol) ethanol being
particularly preferred.
[0014] Extraction conditions may differ depending on the solvent
employed. When the solvent is water, ethanol, or a water-ethanol
mixture, preferably, 1 to 100 parts by weight of solvent is used
for 1 part by weight of plant, and extraction is carried out at 5
to 70.degree. C., preferably 10 to 60.degree. C., for 1 hour to 30
days, preferably for 7 days to 14 days.
[0015] The resultant extract may be used as is, but may also be
used after being processed. For example, the extract may be
diluted, concentrated, or freeze-dried. Optionally, they may
further be processed into a powder or a paste.
[0016] Alternatively, the extract may be used after subjecting to a
liquid-liquid partition or a similar technique, to thereby remove
inert contaminants. In the context of the present invention, use of
an extract which has undergone such a process is preferred. If
desired, the thus-obtained contaminant-free extract may be further
subjected to a conventionally known deodorizing or decoloring
procedure before use.
[0017] In the present invention, a plant or an extract thereof may
be used as a mixture of two or more species.
[0018] As described hereinbelow in the Examples section, in rat
abdominal subcutaneous adipose tissue, a plant or an extract
thereof according to the present invention synergistically
potentiates the lipolysis activity of norepinephrine. Therefore,
through administration of such a plant or extract to a subject of
interest, the body of the subject can be slimmed. Moreover, since
the above activity is also exerted in the visceral adipose tissue,
a composition which contains an effective amount of the plant or
plant extract can serve as a lipolysis stimulator or a slimming
agent which exerts suppressing, preventive or reversal effect
against obesity; or in other words, the slimming effect. In short,
the lipolysis stimulator and the slimming agent of the present
invention stimulate lipolysis of neutral fat stored in adipose
tissue, in particular, subcutaneous fat, to thereby exhibit
suppressing, preventive or reversal effect against obesity; or in
other words, the slimming effect. Therefore, the lipolysis
stimulator and the slimming agent of the present invention can be
used as a cosmetic product, a quasi-drug, a drug, or a food, for
body slimming purposes.
[0019] Although the amount of the active ingredient of the
lipolysis stimulator or the slimming agent may differ depending on,
for example, the form of that ingredient and the manner of
administration, it may be selected from broad ranges. For example,
in the case where a product of external use is the target product,
the active ingredient is incorporated therein in an amount of 0.005
wt. % or more, preferably 0.01 to 30 wt. %, more preferably 0.2 to
25 wt. %, even more preferably 2 to 20 wt. % (all calculated on a
dry basis after extracted with a solvent), with respect to the
entirety of the composition, whereas in the case where an orally
administered product is the target product, the product preferably
contains, in a daily dose for an adult, the active ingredient in an
amount of 0.01 to 10 g, preferably 0.1 to 5 g, more preferably 1.0
to 5 g (all calculated on a dry basis after extracted with a
solvent).
[0020] The lipolysis stimulator or the slimming agent of the
present invention may optionally contain, according to needs, any
suitable combinations of various additives which are used in
cosmetics, quasi-drugs, drugs, foods, or the like fields; or
various pharmaceutically active ingredients. Examples of the
additives include powders such as chalk, talc, fuller's earth,
kaolin, starch, rubber, and colloidal silica sodium polyacrylate;
oils or oily substances such as mineral oil, vegetable oil, and
silicone oil; emulsifiers such as sorbitan trioleate, sorbitan
tristearate, glycerol monooleate, and silicone polymer surfactants;
preservatives such as p-hydroxybenzoate ester; antioxidants such as
butyl hydroxytoluene; wetting agents such as glycerol, sorbitol,
2-pyrrolidone-5-carboxylate, dibutylphthalate, gelatin, and
polyethylene glycol; buffers such as lactic acid-base
(triethanolamine or sodium hydroxide); surfactants such as glycerol
ether, and synthesized, animal, or vegetable ceramides; waxes such
as beeswax, ozokerite wax, and paraffin wax; thickening agents;
activators; colorants; and perfumes. Examples of the
pharmaceutically active ingredients include UV absorbers,
anti-inflammatory agents, germicides, antioxidants, vitamins, and
pharmaceuticals or natural products with fat metabolism promoting
effect or uncoupling protein expression promoting effect (e.g.
xanthine derivatives, .beta.-adrenergic stimulants,
.alpha.-adrenergic inhibitors, bipyridine derivatives, isoflavonic
acid, rosisterol, octacosanol, hydroxytyrosol, grapefruit oil,
raspberry ketone, zingerone, thistle (Cirsium), family Piperaceae,
family Rutaceae, family Menispermaceae, Kigelia plants, Gynostemma
pentaphyllum, Atractylodis lanceae Rhizoma, benzoin (Styrax
benzoin), Coix lacryma-jobi, azuki bean (Vigna angularis (Willd)),
fennel (Foeniculum vulgare), Tabebuia, Geranium thunbergii,
Scutellaria baicalensis, peach, garden thyme (Thymus vulgaris),
Chinese peony (Paeonia lactiflora), tea leaves, Cola acuminata,
Swertia Japonica, Cinnamomi Cortex, Sanguisorba officinalis, sage
(Salvia officinalis), loquat (Eriobotrya Japonica (Thunb.) Lindl.),
bladderwrack (fucus evanescens), carrot, shuitake mashroom,
beefsteak geranium (Saxifraga stolonifera), ginkgo (Ginkgo Biloba),
and other vegetable extracts).
[0021] The lipolysis stimulator and the slimming agent of the
present invention may be formulated into external-use products, or
alternatively, into internal products, injection products, or any
other product forms. Example forms include tablets, capsules,
liquids, powders, granules, creams, milky lotions, jells, pastes,
cataplasms, plasters, sticks, sheets, and tea bags, and they may be
appropriately used for oral administration or for the preparation
of foods, external medicines, bath-additives, or body detergents,
or may be used during taking a shower.
[0022] The slimming method according to the present invention is
characterized by applying to the body the plant or an extract
thereof of the present invention. In the context of the present
invention, the expression "slimming method" means an esthetic
method performed for achieving a slender body with a favorable
look.
EXAMPLES
[0023] The present invention will next be described in more detail
by way of examples.
Production Example 1
[0024] Production of a Common Juniper Extract
[0025] A common juniper extract W (1 mL; product of Maruzen
Pharmaceuticals Co., Ltd.), which is an extract of the fruit of
common juniper with water as a solvent, was thermally processed at
105.degree. C. for 8 hours, whereby a dry matter of the extract (21
mg) was obtained (Invention product 1).
Production Example 2
[0026] Production of a Togenashi Extract
[0027] A togenashi extract G (1 mL; product of Maruzen
Pharmaceuticals Co., Ltd.), which is an extract of the fruit of
togenashi with 50% aqueous 1,3-butylene glycol solution as a
solvent, was thermally processed at 105.degree. C. for 8 hours,
whereby a dry matter of the extract (9 mg) was obtained (Invention
product 2).
Production Example 3
[0028] Production of a Rosehip Extract
[0029] A Pharcolex Rosa canina E (1 mL; product of Ichimaru Pharcos
Co., Ltd.), which is an extract of the fruit of Rosa canina with
50% aqueous ethanol solution as a solvent, was thermally processed
at 105.degree. C. for 8 hours, whereby a dry matter of the extract
(25 mg) was obtained (Invention product 3).
Production Example 4
[0030] Production of an areca Extract
[0031] An extract (1 mL) obtained by extracting seeds of areca (10
g) with 50% aqueous ethanol solution as a solvent was thermally
processed at 105.degree. C. for 8 hours, whereby a dry matter of
the extract (26 mg) was obtained (Invention product 4).
Production Example 5
[0032] Production of a polygala Root Extract
[0033] An extract (1 mL) obtained by extracting roots of polygala
root (10 g) with 50% aqueous ethanol solution as a solvent was
thermally processed at 105.degree. C. for 8 hours, whereby a dry
matter of the extract (126 mg) was obtained (Invention product
5).
Production Example 6
[0034] Production of a plantago Herb Extract
[0035] An extract (1 mL) obtained by extracting the whole plant of
plantago herb (5 g) with 50% aqueous ethanol solution as a solvent
was thermally processed at 105.degree. C. for 8 hours, whereby a
dry matter of the extract (34 mg) was obtained (Invention product
6).
Production Example 7
[0036] Production of a Calumba Extract
[0037] An extract (1 mL) obtained by extracting roots of calumba
(10 g) with 50% aqueous ethanol solution as a solvent was thermally
processed at 105.degree. C. for 8 hours, whereby a dry matter of
the extract (54 mg) was obtained (Invention product 7).
Production Example 8
[0038] Production of a Zuikorodoku Extract
[0039] To one g of roots of zuikorodoku, 10 mL of 50% aqueous
ethanol solution was added, followed by an extraction step at room
temperature for 7 days. The resultant extract was found to contain
an extract of Invention product 8, which is obtained through
thermal treatment at 105.degree. C. for 8 hours, in an amount of
1.24 w/v %.
[0040] Through a similar procedure, respective extracts as shown in
Table 1 (Invention products 9 to 42 and Comparative products 1 to
3) were obtained. In Table 1, "hot water" means that extraction was
performed with hot water for one hour. TABLE-US-00001 TABLE 1
Evaporation residue Sample Plant Extraction solvent (w/v %) Example
garden 50% aqueous ethanol 3.25 product 9 nasturtium Example
kidachiumano- 50% aqueous ethanol 1.50 product 10 suzukusa Example
bayberry 50% aqueous ethanol 1.80 product 11 Example cogon grass
50% aqueous ethanol 2.74 product 12 Example kohon 50% aqueous
ethanol 2.07 product 13 Example shoyokanzo 50% aqueous ethanol 5.36
product 14 Example Japanese white 50% aqueous ethanol 1.45 product
15 birch Example tanjin 50% aqueous ethanol 4.71 product 16 Example
kikubafuro 50% aqueous ethanol 1.46 product 17 Example white
mustard 50% aqueous ethanol 1.47 product 18 Example common 50%
aqueous ethanol 0.82 product 19 sunflower Example ground ivy 50%
aqueous ethanol 3.60 product 20 Example Chinese 50% aqueous ethanol
6.05 product 21 wolfberry Example Japanese pagota 50% aqueous
ethanol 3.61 product 22 tree Example sennenken 50% aqueous ethanol
1.55 product 23 Example common fig 50% aqueous ethanol 3.05 product
24 Example kankatto 50% aqueous ethanol 1.34 product 25 Example
Chinese 50% aqueous ethanol 2.97 product 26 hibiscus Example
usubaakaza 50% aqueous ethanol 1.73 product 27 Example fenugreek
50% aqueous ethanol 1.40 product 28 Example English walnut 50%
aqueous ethanol 0.72 product 29 Example sozuku 50% aqueous ethanol
0.91 product 30 Example koniwa-zakura 50% aqueous ethanol 0.96
product 31 Example gardenia 50% aqueous ethanol 1.91 product 32
Example shima-kan-giku 50% aqueous ethanol 2.93 product 33 Example
akamino-akane 50% aqueous ethanol 1.50 product 34 Example
futaba-mugura 50% aqueous ethanol 1.44 product 35 Example karoou
50% aqueous ethanol 1.13 product 36 Example schizonepeta 50%
aqueous ethanol 1.02 product 37 Example purslane 50% aqueous
ethanol 2.16 product 38 Example karabyakushi 50% aqueous ethanol
1.53 product 39 Example prostrate 50% aqueous ethanol 1.50 product
40 knotweed Example cogon grass hot water 2.98 product 41 Example
cogon grass ethanol 1.88 product 42 Comparative oolong tea hot
water 12.8 product 1 Comparative oolong tea 30% aqueous ethanol
11.9 product 2 Comparative Eucommia leaf hot water 13.1 product 3
tea
Example 1
[0041] The above-described Example products 1 to 42 and Comparative
products 1 to 3 were tested as described below for their lipolysis
stimulating activity. The results are shown in Table 2 (Tables 2-1
and 2-2).
[Test Method]
[0042] The Rodbell's method was used (Rodbell, M., J. Biol. Chem.,
239, 375 (1964)) Specifically, from the abdominal subcutaneous
adipose tissue of each of one to three male Wistar rats (each
weighing 150 to 200 g), isolated adipocytes were prepared by use of
a collagenase solution. The test material was a dried extract or an
extract (liquid form). The prepared cells were incubated at
37.degree. C. for 2 hours in a Hanks buffer solution containing
bovine serum albumin to which the test material and norepinephrine
were added at a concentration of 10 .mu.g/mL on a dry matter basis
and 0.3 .mu.M respectively. The resultant free glycerol was assayed
by the enzyme method. As a control, incubation was carried out in
the presence of norepinephrine alone (without the test material).
Lipolysis stimulation activity was calculated by the following
equation. The results obtained from a double run of test are shown.
Lipolysis stimulation activity (%)=(Released glycerol value in each
group)/(Released glycerol value in control group).times.100
TABLE-US-00002 TABLE 2 Lipolysis stimulation Sample activity (%)
.sup.1) Control 100 Example product 1 221 Example product 2 221
Example product 3 199 Example product 4 186 Example product 5 159
Example product 6 157 Example product 7 150 Example product 8 283
Example product 9 268 Example product 10 341 Example product 11 215
Example product 12 2278 Example product 13 2057 Example product 14
3081 Example product 15 198 Example product 16 192 Example product
17 228 Example product 18 275 Example product 19 256 Example
product 20 233 Example product 21 326 Example product 22 238
Example product 23 269 Example product 24 335 Example product 25
256 Example product 26 267 Example product 27 259 Example product
28 669 Example product 29 1577 Example product 30 1363 Example
product 31 1835 Example product 32 1166 Example product 33 1424
Example product 34 659 Example product 35 793 Example product 36
431 Example product 37 1271 Example product 38 720 Example product
39 1768 Example product 40 1165 Example product 41 1516 Example
product 42 1887 Comparative product 1 105 Comparative product 2 98
Comparative product 3 104 .sup.1) unit = %, The same test was
repeated 2-4 times. In the Table, average values are shown (n = 2
to 3).
[0043] As is apparent from Table 2, when 10 .mu.g/mL of a test
material is applied to dissociated adipocytes, clear lipolysis
stimulating effect was observed in relation to Example products 1
to 42, whereas when any of Comparative products 1 to 3 was used, no
such effect was obtained. Therefore, it has been substantiated that
the Example products exhibit lipolysis stimulating effect on
adipocytes.
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