U.S. patent application number 11/431495 was filed with the patent office on 2007-11-15 for biofilm extracellular polysachharide solvating system.
Invention is credited to Matthew F. Myntti.
Application Number | 20070264296 11/431495 |
Document ID | / |
Family ID | 38685405 |
Filed Date | 2007-11-15 |
United States Patent
Application |
20070264296 |
Kind Code |
A1 |
Myntti; Matthew F. |
November 15, 2007 |
Biofilm extracellular polysachharide solvating system
Abstract
The invention provides a solvating system for the removal of
biofilms which solvates the extracellular polysaccharide matrix
holding it to a surface. The aqueous solvating system comprises
water, a metal ion sequestering agent, and a solvating agent for an
extracellular polysaccharide matrix, which is gentle enough to be
used directly on human tissues, but which may also be used on hard
or soft non-tissue surfaces to breakdown, and/or remove
biofilms.
Inventors: |
Myntti; Matthew F.; (St.
Augustine, FL) |
Correspondence
Address: |
DICKE, BILLIG & CZAJA
FIFTH STREET TOWERS
100 SOUTH FIFTH STREET, SUITE 2250
MINNEAPOLIS
MN
55402
US
|
Family ID: |
38685405 |
Appl. No.: |
11/431495 |
Filed: |
May 10, 2006 |
Current U.S.
Class: |
424/405 ;
424/601; 424/666; 514/557; 514/566; 514/568 |
Current CPC
Class: |
A01N 25/30 20130101;
A61K 45/06 20130101; A61K 31/19 20130101; A61K 33/42 20130101; A61K
33/42 20130101; A61K 31/19 20130101; A61P 27/16 20180101; A61K
33/18 20130101; A01N 25/30 20130101; A61K 33/18 20130101; A61K
31/195 20130101; A01N 37/02 20130101; A61K 2300/00 20130101; A01N
59/14 20130101; A01N 37/06 20130101; A61K 2300/00 20130101; A01N
37/36 20130101; A61K 31/195 20130101; A61P 31/04 20180101; A01N
37/44 20130101; A01N 59/26 20130101; A61K 2300/00 20130101; A01N
37/40 20130101; A01N 37/04 20130101; A01N 37/30 20130101; A01N
59/00 20130101; A01N 37/42 20130101; A01N 37/10 20130101; A61K
2300/00 20130101 |
Class at
Publication: |
424/405 ;
514/557; 514/566; 514/568; 424/601; 424/666 |
International
Class: |
A01N 25/00 20060101
A01N025/00; A61K 31/19 20060101 A61K031/19; A61K 31/195 20060101
A61K031/195; A61K 33/42 20060101 A61K033/42; A61K 33/18 20060101
A61K033/18 |
Claims
1. A solvating system for the breakdown of biofilm matrices,
removal of biofilms from a surface or contained system or both,
said system comprising a solution of water, an metal ion
sequestering agent, and a solvating agent for an extracellular
polysaccharide matrix.
2. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both according to claim 1 wherein
said metal ion sequestering agent is a mild acid having a molarity
of at least about 0.05 molar.
3. An aqueous solvating system for breakdown of biofilm matrices,
removal of biofilms or both according to claim 2 wherein said
sequestering agent is selected from the group consisting of citric
acid, mandelic acid, 2-ketoglutaric acid, acetic acid,
iminodiacetic acid, mucic acid, clycolic acid, fumaric acid, lactic
acid, aspartic acid, phosphoric acid, pyruvic acid, chloroacetic
acid, oxalic acid, oxamic acid, malic acid, dichloroacetic acid,
phenylacetic acid, benzylic acid, maleic acid, succinic acid,
chloromandelic acid, glutamic acid, nitrilotriacetic acid, boric
acid, adipic acid, formic acid, glucuronic acid, salicylic acid,
benzoic acid, benzoyl acid, formic acid, pthalic acid, ketopimelic
acid, and hydrochloric acid.
4. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both according to claim 1 wherein
said solvating agent is selected from the group consisting of
anionic surfactants, nonionic surfactants, cationic surfactants and
zwitterionic surfactants.
5. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both according to claim 1 wherein
said system has a pH of from greater than 5 to about 8.5.
6. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both according to claim 5 wherein
said solvating agent is selected from the group consisting of
anionic surfactants, nonionic surfactants, cationic surfactants and
zwitterionic surfactants.
7. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both according to claim 5 wherein
said system comprises saline.
8. A solvating system for the breakdown of biofilm matrices,
removal of biofilms or both from human tissue, bone, or prosthetic
systems, said system comprising an aqueous solution comprising
water, a metal ion sequestering agent, and a solvating agent for an
extracellular polysaccharide matrix.
9. An aqueous solvating system for breakdown of biofilm matrices,
removal of biofilms or both from human tissue, bone, or prosthetic
systems according to claim 8 wherein said metal ion sequestering
agent is a mild acid having a molarity of at least about 0.05
molar.
10. A solvating system for the breakdown of biofilm matrices,
removal of biofilms or both from human tissue, bone, or prosthetic
systems according to claim 8 wherein said solvating agent is
selected from the group consisting of anionic surfactants, nonionic
surfactants, cationic surfactants and zwitterionic surfactants.
11. A solvating system for the breakdown of biofilm matrices,
removal of biofilms or both from human tissue, bone, or prosthetic
systems according to claim 8 wherein said sequestering agent is
selected from the group consisting of citric acid, mandelic acid,
2-ketoglutaric acid, acetic acid, iminodiacetic acid, mucic acid,
clycolic acid, fumaric acid, lactic acid, aspartic acid, phosphoric
acid, pyruvic acid, chloroacetic acid, oxalic acid, oxamic acid,
malic acid, dichloroacetic acid, phenylacetic acid, benzylic acid,
maleic acid, succinic acid, chloromandelic acid, glutamic acid,
nitrilotriacetic acid, boric acid, adipic acid, formic acid,
glucuronic acid, salicylic acid, benzoic acid, benzoyl acid, formic
acid, pthalic acid, ketopimelic acid, and hydrocholoric acid
12. A solvating system for the removal of biofilms from human
tissue, bone or prosthetic systems according to claim 11 wherein
said solvating agent is selected from the group consisting of alkyl
sulfates, alkyl sulfonates and aryl sulfonates.
13. A solvating system for the removal of biofilms from human
tissue, bone, or prosthetic systems according to claim 8 wherein
said solution has a pH of from greater than 5 to about 8.5.
14. An aqueous solvating system for the breakdown of biofilm
matrices, removal of biofilms or both from human tissue, bone, or
prosthetic systems according to claim 8 wherein said system
comprises saline.
15. A method or procedure for use of an aqueous solvating system
for the breakdown of biofilm matrices, removal of biofilms or both
according to claim 1 wherein a solution of said system is applied
to human tissue, bone, or prosthetic systems.
16. A method of use according to claim 15 wherein said solution is
applied in the form of a spray, liquid, or gel.
17. A method of use according to claim 15 wherein said solution is
applied to nasal passages and sinuses.
18. A method of use according to claim 17 wherein said solution is
applied to persons with rhinosinusitius.
19. A method according to claim 18 wherein said solution is applied
in the form of a spray, liquid, or gel.
20. A method of treatment of medical disorders by removal of
biofilms from human tissues, bone, or prosthetic systems, said
method comprising: a. providing an aqueous solvating system
solution according to claim 1, b. applying said solution directly
to said human tissue comprising said biofilm thereon, c. removing
said solution by means selected from draining, flushing, aspiration
and rinsing.
21. A method of treating rhinosinusitis, otitis, and dental
disorders comprising the steps of: a. providing an aqueous
solvating system solution according to claim 1, b. applying said
solution directly to a human tissue selected from nasal passages,
sinuses, internal and external ears, gums, tongue, and teeth, and
c. allowing said solution to drain out of said human tissue.
Description
THE FIELD OF THE INVENTION
[0001] The present invention relates generally to the field of
biofilm removal and more specifically to a solvating system for the
solvation and removal of biofilms. The solvating system may be used
on a variety of affected surfaces, including human or animal
tissue, medical devices, water systems, and the like. The solvating
system is especially beneficial for use on human tissue to remove
biofilms which cause chronic conditions such as rhinosinusitis.
BACKGROUND OF THE INVENTION
[0002] Biofilms are formed by bacteria in aqueous environments,
which interact with the surfaces to which they are exposed to form
surface colonies and films which continue to adhere to the surfaces
and grow. More specifically, the bacteria produce extensive
exopolysaccharide or extracellularpolysaccharide polymers (EPS or
ECPS) that keep them attached to the surfaces and form living films
thereon, frequently called "biofilms". These biofilms can be formed
on a variety of surfaces, including human tissues, medical devices,
dental office equipment, counters, pipes and the like. Biofilms
coat the surface and become a living colony for the continued
proliferation of microorganisms, and protection of the
microorganisms from removal and from conditions which might destroy
the microorganisms. Biofilms are much more difficult to remove than
bacteria in the plaktonic state, and the bacterial contamination of
the biofilms from surfaces or tissue are thus much more difficult
to eliminate. Biofilms in this state are extremely resistant to
many antibiotics and biocides.
[0003] When present on human tissues, biofilms can cause chronic
conditions from which many persons today suffer. Such conditions
include rhinosinusitis, where biofilms are attached within the
nasal passages and sinuses, infiltrating and protecting the
underlying pathogenic bacteria and preventing them from being
dislodged from there surfaces, and immune system disorder symptoms
where biofilms have coated bodily tissues and surfaces such as
joints or nerves in a manner which impairs the normal function
thereof. Additionally, enclosure of implants or surgical appliances
with biofilms may lessen their effectiveness.
[0004] Previous strategies for removal of biofilms have focused on
both removal and destruction of bacteriums in the biofilm. Products
for such cleansing, such as biocides, disinfectants and the like
for use in such areas may be caustic and employ agents that can
damage human skin, and especially non-dermal human tissues upon
contact and therefore cannot be used to remove biofilms from human
orifices and tissues. Methods of cleaning and disinfecting such
surfaces effectively for biofilms may also involve large dosages,
and long periods of contact with the surface, e.g., soaking biofilm
contaminated surfaces for 12-24 hours, which is impractical for
preoperative and postoperative situations and for many surfaces and
devices as well as being impossible for use on or with most
contaminated human tissues.
[0005] Methods of removal of biofilms from human tissues such as
sinuses to flush them from the system have included mechanical
debridement of the tissues and/or surgical opening of sinuses to
allow for drying and subsequent removal of the biofilm. Such
methods damage the tissues and require healing periods, and further
present opportunities for new bacteria to contact the surfaces and
cause infections and new placement of biofilms. Antibiotics have
also been attempted but, while they are effective against plaktonic
bacteria, they have been only marginally effective against
biofilms, and then only when administered in large dosages, which
may be otherwise undesirable for the patient or living tissues.
[0006] It would be desirable to have a solvating system for
removing biofilms from human tissues which would meet
biocompatibility requirements for contact with human tissue, and
yet be effective in removal of such biofilms from tissues and
bodily orifice linings such as nasal orifices, sinuses, oral
tissues, for removal from implants or other appliances attached to
bodily tissues and the like. Such desirable solvating systems would
preferably be effective in small dosages for short periods of
application. It would also be desirable for such solvating system
to be further useful to dislodge biofilms attached to non-tissue
surfaces in environmental locations such as medical devices and
water systems, dental equipment and the like.
[0007] It has now been discovered that a solvating system
comprising an alkali, metallic, or metal ion sequestering agent and
a solvent or surfactant is surprisingly effective in removal of
biofilms such as polysaccharides from human tissue while being
gentle enough for application directly onto such tissues.
SUMMARY OF THE INVENTION
[0008] The invention provides a solvating system for the breakdown
and/or removal of biofilm matrices from human tissue surfaces and
nonhuman surfaces.
[0009] More specifically, the invention provides an aqueous
solvating system for the breakdown of the biofilm's extracellular
polysaccharide matrix, and consequent detachment/removal of
biofilms from the surface to which it is attached or adhered. The
solvating system of the invention comprises a metal ion
sequestering agent and a solvating agent for the extracellular
polysaccharide matrix selected from a solvent or a surfactant.
[0010] In one embodiment, the invention includes an aqueous
solvating system comprising water or saline, a metal ion
sequestering agent and a solvating agent selected from the group
consisting of a solvent and a surfactant.
[0011] In another embodiment, the solvating system of the invention
comprises metal ion sequestering agent selected from the group
consisting of a mild acid having a molarity of at least about 0.05
molar.
[0012] In one embodiment, the metal ion sequestering agent is a
mild acid having a molarity of at least about 0.05 molar wherein
the metal ion is selected from alkali metals, alkaline earth
metals, and iron.
[0013] In another embodiment, the solvating system comprises a
solvating agent for the extracellular polysaccharide matrix
selected from the group consisting of anionic surfactants, nonionic
surfactants, cationic surfactants and zwitterionic surfactants.
[0014] The invention also provides a method of use for the
solvating system comprising delivery to the affected location by
power spray or lavage.
[0015] These terms when used herein have the following
meanings.
[0016] 1. The term "sequestering agent" means a chemical that will
combine with another material, especially a metal ion, to prevent
the material from coming out of solution.
[0017] 2. The term "metal ion sequestering agent" means a
sequestering agent that will combine with metal ions such as iron,
alkali metals, alkaline earth metals, and the like to keep the
metals in solution. In order of increasing atomic number the
alkaline earth metals are beryllium, magnesium, calcium, strontium,
barium, and radium. Alkali metals include sodium, potassium,
rubidium, cesium, and francium.
[0018] 3. The terms "attached" and "adhered" as used herein means
that the biofilm is established on the surface which it coats or
covers, and that the biofilm has some resistance to removal from
the surface, whether the surface is living tissue or a nonliving
surface. As the nature of this relationship is complex and poorly
understood, no particular method of adherence or attachment is
intended by such usage.
[0019] 4. The term "solvating" means to form a solution consisting
of the solvent and the solvate.
[0020] 5. The term "removal of biofilms" means that at least a
significant amount of the biofilm present on a surface is placed
into suspension and no longer resides on the surface.
[0021] All weights, amounts and ratios herein are by weight, unless
otherwise specifically noted.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0022] The following detailed description describes certain
embodiments and is not to be taken in a limiting sense. The scope
of the present invention is defined by the appended claims.
[0023] The invention provides an aqueous solvating system for the
breakdown of the biofilm's extracellular polysaccharide matrix, and
consequent detachment or removal of biofilms from the surface to
which it is attached or adhered. The solvating system of the
invention comprises a metal ion sequestering agent and a solvating
agent for the extracellular polysaccharide matrix selected from a
solvent or a surfactant.
[0024] The invention is biocompatible, and may be used directly on
human tissue as well as other non-living surfaces. It is
advantageous in that it contains no biocides which could be
potentially harmful to human tissues.
[0025] Another advantage of the solvating system invention is that
it is low viscosity which makes for easy delivery to the desired
surface by means of lavage, misting, spray application, mopping,
administering in droplets, and also easy removal by subsequently
flushing, rinsing, and/or draining from orifices such as nasal
passages or from other surfaces. In one embodiment, the solvating
system has a pH of from greater than about 5 to about 8.5.
[0026] The sequestering agent is an metal ion sequestering agent,
generally a mild acid of high molarity. Useful acids include citric
acid, mandelic acid, 2-ketoglutaric acid, acetic acid,
iminodiacetic acid, mucic acid, clycolic acid, fumaric acid, lactic
acid, aspartic acid, phosphoric acid, pyruvic acid, chloroacetic
acid, oxalic acid, oxamic acid, malic acid, dichloroacetic acid,
phenylacetic acid, benzylic acid, maleic acid, succinic acid,
chloromandelic acid, glutamic acid, nitrilotriacetic acid, boric
acid, adipic acid, formic acid, glucuronic acid, salicylic acid,
benzoic acid, benzoyl acid, formic acid, pthalic acid, ketopimelic
acid, and hydrochloric acid.
[0027] Applicable metal ions which may be sequestered include
alkali metals, alkaline earth metals, iron, and the like. In one
embodiment the metal ion sequestering agent is an alkaline earth
metal or alkali metal sequestering agent. The sequestering agent
generally has a molarity of at least about 0.05 molar, preferably
from about 0.05 to about 0.35 molar.
[0028] The solvating system further includes a solvating agent
selected from a surfactant or solvent. Useful solvating agents
include surfactants such as alkyl sulfates, alkyl sulfonates and
aryl sulfonates. The surfactant is generally present in a strength
of from about 0.001 to about 0.69 molar, preferably from about
0.025 to about 0.130 molar, and in an amount of from about 0.5% to
about 20% of the weight of the solution.
[0029] The solvating agent may be selected from various
surfactants, such as anionic surfactants, nonionic surfactants,
cationic surfactants and zwitterionic surfactants. Useful anionic
surfactants include but are not limited to, sodium
chenodeoxycholate, N-lauroylsarcosine sodium salt, lithium dodecyl
sulfate, 1-octanesulfonic acid sodium salt, sodium cholate hydrate,
sodium deoxycholate, sodium dodecyl sulfate, and sodium
glycodeoxycholate. Useful cationic surfactants include but are not
limited to hexadecylpyridinium chloride monohydrate, and
hexadecyltrimethylammonium bromide. Useful nonionic surfactants
include but are not limited to polyoxyethyleneglycol dodecyl ether,
N-decanoyl-N-methylglucamine, Digitonin, n-dodecyl B-D-maltoside,
octyl B-D-glucopyranoside, octylphenol ethoxylate, polyoxyethylene
(8) isooctyl phenyl ether, polyoxyethylene sorbitan monolaurate,
and polyoxyethylene (20) sorbitan monooleate. Useful zwitterionic
surfactant include but are not limited to
3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propane
sulfonate, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane
sulfonate, 3-(decyldimethlammonio)propanesulfonate inner salt, and
N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate. In one
embodiment, the surfactant is sodium lauryl sulfate.
[0030] While not wishing to be bound by theory, it is believed that
the metal ion sequestering material removes the ion which bridges
the extracellular polysaccharide matrix and binds the polymer
chains together. The solvating agent then first surrounds the
unbound polymers and suspends them, breaking down the matrix, and
subsequently solvates the unbound polymers, bringing them into
solution where they can be easily flushed from the tissues or
surfaces with the aqueous solvating solution.
[0031] Where desirable to both remove the biofilm and destroy the
microorganisms contained therein, the solvating system of the
invention may further include medicaments such as antibiotics,
which will be much more effective against the microorganisms
present after the extracellular polysaccharide matrix has been
broken down into unbound polymers, suspended and/or solvated.
[0032] The solvating system may further include a buffer in order
to provide a solution at the proper pH for contacting human tissue.
Where desirable, the buffer may comprise up to about 25% of the
active ingredients of the solution. Useful buffers include, but are
not limited to potassium chloride, glycine, potassium hydrogen
phthalate, sodium acetate, potassium hydrogen phthalate, barbitone
sodium, and sodium citrate.
[0033] Where treatment of tissues is also desirable, the solvating
system of the invention may include further pharmaceutical agents
in appropriate dosages such as analgesics, steroids, and the
like.
[0034] For comfort and ease of use in human patients, the solvating
system may further include flavoring agents and sweetening agents
including but not limited to, oil of peppermint, spearmint,
wintergreen, clove, eucalyptus, cinnamon, lemon, lime and orange,
cherry, sucrose, lactose, maltose, sorbitol, xylitol, sodium
cyclamate, saccharine, and the like.
[0035] Additional adjuvants may include antioxidants, buffering
agents, coloring agents, and the like, in amounts that will not
substantially interfere with the solvation of the extracellular
polysaccharide and removal of the significant amounts of the
biofilm.
[0036] Although specific embodiments have been illustrated and
described herein for purposes of description of the preferred
embodiment, it will be appreciated by those of ordinary skill in
the art that a wide variety of alternate and/or equivalent
implementations calculated to achieve the same purposes may be
substituted for the specific embodiments shown and described
without departing from the scope of the present invention. Those
with skill in the chemical, mechanical, electro-mechanical,
electrical, pharmacological and computer arts will readily
appreciate that the present invention may be implemented in a very
wide variety of embodiments. This application is intended to cover
any adaptations or variations of the preferred embodiments
discussed herein. Therefore, it is manifestly intended that this
invention be limited only by the claims and the equivalents
thereof.
* * * * *