Methods for Improving Seed Characteristics

Abstract

Methods are provided for altering grain characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered number, type, or configuration of aleurone cells within the seed. Also provided are isolated nucleic acids that encode maize superal proteins, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of co-pending application U.S. Application Ser. No. 10/208,948 filed Jul. 30, 2002 which claims priority to and the benefit of U.S. Provisional Application Ser. No. 60/309,719 filed Aug. 2, 2001 and U.S. Provisional Application Ser. No. 60/337,444 filed Oct. 25, 2001, the contents of which are incorporated herein by reference.

TECHNICAL FIELD

[0002] The present invention relates to the field of crop improvement. Specifically, the present invention relates to the identification and use of genes encoding molecules involved in altering the number and characteristics of aleurone cells in plant seeds and the use of these genes and mutants thereof to improve nutritional, industrial, and agricultural traits in seed and grain.

BACKGROUND OF THE INVENTION

[0003] Most common cereal grains contain cells called aleurone cells that occur as the most external layer of the endosperm. Aleurone cells are generally oil and protein-rich and secrete enzymes allowing the mobilization of endosperm reserves during seed germination. Barley, oats, and some varieties of rice have been reported to have 2 to 4 layers of aleurone cells (Sawicki et al., 1952, and Hoshikawa et al., 1967). Maize commonly has only a single layer making up only 2% or less of the weight of the kernel (Hinton et al., 1953). A South American race of maize, Coroico, has been found to contain from 2 to 6 layers of aleurone cells (Wolf et al., 1972). This characteristic has been referred to as the MaI (multiple aleurone) phenotype by Welch et al., 1993.

[0004] When compared to grains having only a single layer of aleurone cells, varieties having multiple aleurone cell layers contain increased amounts of minerals (Welch et al., 1993) and protein (Wolf, ibid). However, the MaI phenotype is inherited as a partially dominant trait and is highly variable in the Coroico line. To date, a reliable, multiple aleurone trait has not been available in maize or other cereal crops that could be used in a breeding program to take advantage of the unique characteristics of aleurone cells.

[0005] Thus, a transgenic or breeding approach that alters the number of aleurone cells in a grain crop as well as the characteristics of the aleurone cells would be highly desirable.

SUMMARY OF THE INVENTION

[0006] Several genes have been isolated that are known to affect the differentiation of the aleurone layer in crop seeds. This invention foresees using these nucleic acids, or polypeptides, or variants thereof, to improve seed and grain quality by altering the number, type, and/or characteristics of aleurone cells.

DETAILED DESCRIPTION OF THE INVENTION

[0007] Units, prefixes, and symbols may be denoted in their Si accepted form. Unless otherwise indicated, nucleic acids are written left to right in 5' to 3' orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively. Numeric ranges recited within the specification are inclusive of the numbers defining the range and include each integer within the defined range. Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes. Unless otherwise provided for, software, electrical, and electronics terms as used herein are as defined in The New IEEE Standard Dictionary of Electrical and Electronics Terms (5th edition, 1993). The terms defined below are more fully defined by reference to the specification as a whole.

[0008] By "seed" or "kernel" is intended to include the grain or ripened ovule of a plant, or more broadly, a propagative plant structure. The terms "seed" and "kernel" are used interchangeably herein.

[0009] The term "grain" as used herein means the mature seed produced by commercial growers for purposes other than growing or reproducing the species.

[0010] As used herein the term "recombinant" means having parts combined in a configuration not found in nature.

[0011] The term "coding sequence" or "coding region" refers to a nucleotide sequence that codes for a specific amino acid sequence.

[0012] The term "expression" as used herein, refers to the transcription and accumulation of sense (mRNA) or antisense RNA derived from a nucleic acid fragment of the invention. Expression may also refer to translation of mRNA into a polypeptide.

[0013] The term "isolated" refers to material, such as a nucleic acid or a protein, which is: (1) substantially or essentially free from components which normally accompany or interact with the material as found in its naturally occurring environment or (2) if the material is in its natural environment, the material has been altered by deliberate human intervention to a composition and/or placed at a locus in the cell other than the locus native to the material.

[0014] As used herein, the term "nucleic acid" means a polynucleotide and includes single or multi-stranded polymers of deoxyribonucleotide or ribonucleotide bases. Nucleic acids may also include fragments and modified nucleotides. Therefore, as used herein, the terms "nucleic acid" and "polynucleotide" are used interchangably.

[0015] As used herein, "polypeptide" means proteins, protein fragments, modified proteins (e.g., glycosylated, phosphorylated, or other modifications), amino acid sequences and synthetic amino acid sequences. The polypeptide can be modified or not. Therefore, as used herein, "polypeptide" and "protein" are used interchangably.

[0016] As used herein, "plant" includes plants and plant parts including but not limited to plant cells and plant tissues such as leaves, stems, roots, flowers, pollen, and seeds.

[0017] As used herein, "promoter" includes reference to a region of DNA upstream from the start of transcription and involved in recognition and binding of RNA polymerase and other proteins to initiate transcription.

[0018] By "fragment" is intended a portion of the nucleotide sequence or a portion of the amino acid sequence and hence protein encoded thereby. Fragments of a nucleotide sequence may encode protein fragments that retain the biological activity of the native nucleic acid ie: "functional fragments". Alternatively, fragments of a nucleotide sequence that can be useful as hybridization probes may not encode fragment proteins retaining biological activity. Thus, fragments of a nucleotide sequence are generally greater than 25, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, or 700 nucleotides and up to and including the entire nucleotide sequence encoding the proteins of the invention. Generally the probes are less than 1000 nucleotides and often less than 500 nucleotides. Fragments of the invention include antisense sequences used to decrease expression of the inventive polynucleotides. Such antisense fragments may vary in length ranging from greater than 25, 50, 100, 200, 300, 400, 500, 600, or 700 nucleotides and up to and including the entire coding sequence.

[0019] By "functional equivalent" as applied to a polynucleotide or a protein is intended a polynucleotide or a protein of sufficient length to modulate the level of protein activity in a plant cell. A polynucleotide functional equivalent can be in sense or antisense orientation.

[0020] By "variants" is intended substantially similar sequences. Generally, nucleic acid sequence variants of the invention will have at least 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the native nucleotide sequence, wherein the % sequence identity is based on the entire sequence and is determined by GAP 10 analysis using default parameters. Generally, polypeptide sequence variants of the invention will have at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 98% sequence identity to the native protein, wherein the % sequence identity is based on the entire sequence and is determined by GAP 10 analysis using default parameters. GAP uses the algorithm of Needleman and Wunsch (J. Mol. Biol. 48:443-453,1970) to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps.

[0021] As used herein "transformation" includes stable transformation and transient transformation.

[0022] As used herein "stable transformation" refers to the transfer of a nucleic acid fragment into a genome of a host organism (this includes both nuclear and organelle genomes) resulting in genetically stable inheritance. In addition to traditional methods, stable transformation includes the alteration of gene expression by any means including chimeraplasty or transposon insertion.

[0023] "Non-ruminant animal" means an animal with a simple stomach divided into the esophageal, cardia, fundus and pylorus regions. A non-ruminant animal additionally implies a species of animal without a functional rumen. A rumen is a section of the digestive system where feedstuff/food is soaked and subjected to digestion by microorganisms before passing on through the digestive tract. This phenomenon does not occur in a non-ruminant animal. The term non-ruminant animal includes, but is not limited to: humans, swine, poultry, cats and dogs.

[0024] The term "aleurone cell" as used herein refers to an epidermis-like cell layer in the seeds of many crop species. This aleurone layer functions as "an important source of hydrolytic enzymes required for remobilization of stored starch and protein during germination" (Becraft et al., Science, 1996 273:1406-1409.) Wild-type aleurone cells contain oil bodies, phytic acid inclusions and protein carbohydrate bodies. Storage proteins are in the form of globulins. Wild-type aleurone cells contain very little or no starch granules. As a seed matures, aleurone cells go through a maturation and desiccation program. After seed desiccation, mature aleurone cells respond to GA stimulation by initiation of transcription of genes encoding amylases, proteases and glucanases upon inhibition. A marker of aleurone cells is that they can activate transcription from the barley Ltp2 promoter (Kalla, R. et al., 1994). Sudan red can be used to visualize the high concentration of oil bodies in the aleurone layer, as well as the low amount of oil bodies in nearby starchy endosperm cells. Aleurone cells may also be transgenically manipulated to alter expression in--including but not limited to--type or amount of: oil bodies and their contents, storage proteins, protein-carbohydrate bodies, phytate deposition, and responsiveness to GA in the form of transcriptional activation of one or more of the genes activated in germinating seeds.

[0025] It is expected that modulating the expression of the nucleic acids of the present invention will modulate signaling in the aleurone cell developmental pathway providing methods to alter the number and characteristics of the aleurone cells.

[0026] Increasing the aleurone cell number is a mechanism for increasing the concentration of plant sterols (phytosterols) and their derivatives. Phytosterols have been shown to have important applications to human health by, for example, decreasing serum cholesterol concentrations. Sitostanol, a sterol derivative that is particularly effective in reducing cholesterol, is found at unusually high concentrations in maize aleurone cells.

[0027] As used herein, "Dek1" is a polynucleotide of the present invention that encodes a polypeptide which is a protein involved in aleurone cell differentiation, and refers to one or more amino acid sequences, in modified or unmodified form, that is necessary and sufficient for aleurone cell fate determination in a transformed plant. The term is also inclusive of fragments, variants, homologs, alleles or precursors (e.g., preproproteins or proproteins) that, when introduced into a plant or plant germ line by way of--but not limited to--transformation or breeding, result in changes in the aleurone cells.

[0028] The Dek1 gene has been identified as a calpain based on the presence of the cystein proteinase domain shared by all members of the calpain super gene family. The sequence of the single copy gene ZmDek1-calpain spans about 24 000 bp (SEQ ID NO:25). A transcriptional start-site is predicted at bp1440 and the coding sequence is between bp 2682-23732, containing 31 exons. Arabidopsis thaliana also has a single copy Dek1-calpain orthologue (SEQ ID NO:28) that shares a remarkable conservation with the maize gene, including an identical exon structure.

[0029] The maize Dek1 coding region is 7110 bp, compared with 6453 bp for Arabidopsis. Based on MPSS transcript profiling technology (Lynx Therapeutics, Inc. 25861 Industrial Blvd. Hayward, Calif. 94545). Maize Dek1 is expressed at a low level in many tissues of the maize plant, including kernel, roots, tassel, stem sheaths, leaf, seedlings and roots. Using RT-PCR, the transcript is detected in unfertilized ovules and in developing grains up to 15 DAP, but very little at 22 DAP.

[0030] The isolated maize dek1 promoter region is characterized by a putative TATA box at positions 2283-2287 of NO: 25 and a putative CAAT box at positions 2333-2337 of NO: 25.

[0031] Kernels of the dek1 corn mutant lack aleurone cells and the endosperm of such mutants consists of starchy endosperm cells in the periphery normally occupied by aleurone cells. Positional cues specify and maintain aleurone cell fate in maize endosperm development. A reversion of a dek1 mutant allele to wild-type restores aleurone cell identity to peripheral starchy endosperm cells in which this event occurs (Becraft et al., Development, 127(18):4039-48, 2000).

[0032] The maize DEK1 protein is 2159 amino acid residues (239.0 kDa) and 2151 amino acids for Arabidopis: the two protein sharing 70% overall identity. The DEK1 proteins are identified as members of the calpain superfamily by their conserved cystein proteinase domain 11 (from aa position 1708-2013 of SEQ ID NO:24) shared by all members of this superfamily, plus similarity in domain III (from aa position 2014-2163) present in many calpains. The identity between maize and Arabidopsis in these two domains is 88 and 83%, respectively.

[0033] DEK1 proteins from maize and Arabidopsis differ from conventional calpains by their N-terminal extensions of 1435 amino acids. This part of the protein contains five distinguishable domains, including a predicted plasma membrane targeting signal in the N-terminus (positions 1-30 of SEQ ID NO:24). This region is followed by a predicted membrane spanning peptide sequence (positions 31-335 of SEQ ID NO:24). A similar domain, containing 15 predicted membrane-spanning peptide sequences, is found further downstream (positions 620-1108 of SEQ ID NO:24). The identity between maize and Arabidopsis in these two regions is 72% and 64%, respectively. The two membrane spanning domains are separated by a loop region (positions 336-628 of SEQ ID NO:24). This region is the least conserved part of the maize and Arabidopis DEK1 proteins, with 57% identity.

[0034] Over-expression or modification of this protein can increase the number of aleurone layer cells, or the production of aleurone cells in parts of the seed that do not normally possess these cells. Further, creation of a dominant version of the dek1 protein would render the signal transduction pathway responsible for aleurone cell fate specification constitutively active in the cells expressing the gene--inducing formation of aleurone layers independent of the positional cues proposed to be involved in aleurone cell formation.

[0035] The crinkly4 (cr4)(Becraft, P. W., Science, 1996, 273:1406-1409) gene encodes a putative receptor kinase that is involved in the signaling pathway controlling aleurone layer differentiation. Over-expression or modification of this protein can increase the number of aleurone layer cells.

[0036] A dominant version of cr4 can be obtained by functional screening mutated cr4 gene. The cr4 kinase domain as described in Becraft can be fused to the extracellular domain of the clv1 (Clark S. E., et al. Cell. May 16, 1997;89(4):575-85) or bri1 (Wang Z. Y., et al, Nature. Mar. 15, 2001;410(6826):380-3) gene to form a chimeric receptor that can be activated by CLV3 (Trotochaud A. E., et al, Science Jul. 28, 2000 289(5479):613-7) or brassinosteroid.

[0037] As used herein "Superal" is a polynucleotide of the present invention that encodes a polypeptide involved in or interacts with the signal transduction pathway that determines aleurone cell fate. The kernels of superal mutants are characterized by as many as eight layers of aleurone cells. Down regulation of this protein can increase the number of aleurone cells in the seed.

Nucleic Acids

[0038] The isolated nucleic acids of the present invention can be made using standard recombinant methods, synthetic techniques, or combinations thereof known in the art. In some embodiments, the polynucleotides of the present invention can be cloned, amplified, or otherwise constructed from monocots or dicots. Typical plants include maize, barley, wheat, rice, rye, oats, millet, soybeans, arabidopsis, tobacco, canola, sunflower, or sorghum.

[0039] Functional fragments included in the invention can be obtained using primers which selectively hybridize under stringent conditions. Primers are generally at least 12 bases in length and can be as high as 200 bases, but will generally be from 15 to 75, or more likely from 15 to 50 bases. Functional fragments can be identified using a variety of techniques such as restriction analysis, Southern analysis, primer extension analysis, and DNA sequence analysis.

[0040] The present invention includes a plurality of polynucleotides that encode for the DEK1 amino acid sequence. The degeneracy of the genetic code allows for such "silent variations" which can be used, for example, to selectively hybridize and detect allelic variants of polynucleotides of the present invention. Additionally, the present invention includes isolated nucleic acids comprising allelic variants. The term "allele" as used herein refers to a related nucleic acid of the same gene.

[0041] Variants of nucleic acids included in the invention can be obtained, for example, by oligonucleotide-directed mutagenesis, linker-scanning mutagenesis, mutagenesis using the polymerase chain reaction, and the like. See, for example, pages 8.0.3-8.5.9 Current Protocols in Molecular Biology, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995). Also, see generally, McPherson (ed.), DIRECTED MUTAGENESIS: A Practical Approach, (IRL Press, 1991). Thus, the present invention also encompasses DNA molecules comprising nucleotide sequences that have substantial sequence similarity with the inventive sequences.

[0042] Variants included in the invention may contain individual substitutions, deletions or additions to the nucleic acid or polypeptide sequences which alter, add or delete a single amino acid or a small percentage of amino acids in the encoded sequence. A "conservatively modified variant" is an alteration which results in the substitution of an amino acid with a chemically similar amino acid. When the nucleic acid is prepared or altered synthetically, advantage can be taken of known codon preferences of the intended host.

[0043] The present invention also includes "shufflents" produced by sequence shuffling of the inventive polynucleotides to obtain a desired characteristic. Sequence shuffling is described in PCT publication No. 96/19256. See also, Zhang, J. H., et al., Proc. Natl. Acad. Sci. USA 94:4504-4509 (1997).

[0044] The present invention also includes a promoter region natively associated with the maize dek1 coding region. By "natively associated" is meant a promoter region as it exists prior to its isolation from its associated in vivo coding region.

[0045] The present invention also includes the use of 5' and/or 3' UTR regions for modulation of translation of heterologous coding sequences. Positive sequence motifs include translational initiation consensus sequences (Kozak, Nucleic Acids Res. 15:8125 (1987)) and the 7-methylguanosine cap structure (Drummond et al., Nucleic Acids Res. 13:7375 (1985)). Negative elements include stable intramolecular 5' UTR stem-loop structures (Muesing et al., Cell 48:691 (1987)) and AUG sequences or short open reading frames preceded by an appropriate AUG in the 5' UTR (Kozak, supra, Rao et al., Mol. and Cell. Biol. 8:284 (1988)).

[0046] Further, the polypeptide-encoding segments of the polynucleotides of the present invention can be modified to alter codon usage. Altered codon usage can be employed to alter translational efficiency. Codon usage in the coding regions of the polynucleotides of the present invention can be analyzed statistically using commercially available software packages such as "Codon Preference" available from the University of Wisconsin Genetics Computer Group (see Devereaux et al., Nucleic Acids Res. 12:387-395 (1984)) or MacVector 4.1 (Eastman Kodak Co., New Haven, Conn.).

[0047] For example, the inventive nucleic acids can be optimized for enhanced expression in plants of interest. See, for example, EPA0359472; WO91/16432; Perlak et al. (1991) Proc. Natl. Acad. Sci. USA 88:3324-3328; and Murray et al. (1989) Nucleic Acids Res. 17:477-498. In this manner, the polynucleotides can be synthesized utilizing plant-preferred codons. See, for example, Murray et al. (1989) Nucleic Acids Res. 17:477-498, the disclosure of which is incorporated herein by reference.

[0048] The present invention provides subsequences comprising isolated nucleic acids containing at least 20 contiguous bases of the inventive sequences. For example the isolated nucleic acid includes those comprising at least 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700 or 800 contiguous nucleotides of the inventive sequences. Subsequences of the isolated nucleic acid can be used to modulate or detect gene expression by introducing into the subsequences compounds which bind, intercalate, cleave and/or crosslink to nucleic acids.

[0049] The nucleic acids of the invention may conveniently comprise a multi-cloning site comprising one or more endonuclease restriction sites inserted into the nucleic acid to aid in isolation of the polynucleotide. Also, translatable sequences may be inserted to aid in the isolation of the translated polynucleotide of the present invention. For example, a hexa-histidine marker sequence provides a convenient means to purify the proteins of the present invention.

[0050] A polynucleotide of the present invention can be attached to a vector, adapter, promoter, transit peptide or linker for cloning and/or expression of a polynucleotide of the present invention. Additional sequences may be added to such cloning and/or expression sequences to optimize their function in cloning and/or expression, to aid in isolation of the polynucleotide, or to improve the introduction of the polynucleotide into a cell. Use of cloning vectors, expression vectors, adapters, and linkers is well known and extensively described in the art. For a description of such nucleic acids see, for example, Stratagene Cloning Systems, Catalogs 1995, 1996, 1997 (La Jolla, Calif.); and, Amersham Life Sciences, Inc, Catalog '97 (Arlington Heights, Ill.).

[0051] The isolated nucleic acid compositions of this invention, such as RNA, cDNA, genomic DNA, or a hybrid thereof, can be obtained from plant biological sources using any number of cloning methodologies known to those of skill in the art. In some embodiments, oligonucleotide probes which selectively hybridize, under stringent conditions, to the polynucleotides of the present invention are used to identify the desired sequence in a cDNA or genomic DNA library.

[0052] Exemplary total RNA and mRNA isolation protocols are described in Plant Molecular Biology: A Laboratory Manual, Clark, Ed., Springer-Verlag, Berlin (1997); and, Current Protocols in Molecular Biology, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995). Total RNA and mRNA isolation kits are commercially available from vendors such as Stratagene (La Jolla, Calif.), Clonetech (Palo Alto, Calif.), Pharmacia (Piscataway, N.J.), and 5'-3' (Paoli, Pa.). See also, U.S. Pat. Nos. 5,614,391; and, 5,459,253.

[0053] Typical cDNA synthesis protocols are well known to the skilled artisan and are described in such standard references as: Plant Molecular Biology: A Laboratory Manual, Clark, Ed., Springer-Verlag, Berlin (1997); and, Current Protocols in Molecular Biology, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995). cDNA synthesis kits are available from a variety of commercial vendors such as Stratagene or Pharmacia.

[0054] An exemplary method of constructing a greater than 95% pure full-length cDNA library is described by Carninci et al., Genomics 37:327-336 (1996). Other methods for producing full-length libraries are known in the art. See, e.g., Edery et al., Mol. Cell Biol. 15(6):3363-3371 (1995); and PCT Application WO 96/34981.

[0055] It is often convenient to normalize a cDNA library to create a library in which each clone is more equally represented. A number of approaches to normalize cDNA libraries are known in the art. Construction of normalized libraries is described in Ko, Nucl. Acids. Res. 18(19):5705-5711 (1990); Patanjali et al., Proc. Natl. Acad. U.S.A. 88:1943-1947 (1991); U.S. Pat. Nos. 5,482,685 and 5,637,685; and Soares et al., Proc. Natl. Acad. Sci. USA 91:9228-9232 (1994).

[0056] Subtracted cDNA libraries are another means to increase the proportion of less abundant cDNA species. See, Foote et al., in Plant Molecular Biology: A Laboratory Manual, Clark, Ed., Springer-Verlag, Berlin (1997); Kho and Zarbl, Technique 3(2):58-63 (1991); Sive and St. John, Nucl. Acids Res. 16(22):10937 (1988); Current Protocols in Molecular Biology, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995); and, Swaroop et al., Nucl. Acids Res. 19(8):1954 (1991). cDNA subtraction kits are commercially available. See, e.g., PCR-Select (Clontech).

[0057] To construct genomic libraries, large segments of genomic DNA are generated by random fragmentation. Examples of appropriate molecular biological techniques and instructions are found in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Vols. 1-3 (1989), Methods in Enzymology, Vol.152: Guide to Molecular Cloning Techniques, Berger and Kimmel, Eds., San Diego: Academic Press, Inc. (1987), Current Protocols in Molecular Biology, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995); Plant Molecular Biology: A Laboratory Manual, Clark, Ed., Springer-Verlag, Berlin (1997). Kits for construction of genomic libraries are also commercially available.

[0058] The cDNA or genomic library can be screened using a probe based upon the sequence of a nucleic acid of the present invention such as those disclosed herein. Probes may be used to hybridize with genomic DNA or cDNA sequences to isolate homologous polynucleotides in the same or different plant species. Those of skill in the art will appreciate that various degrees of stringency of hybridization can be employed in the assay; and either the hybridization or the wash medium can be stringent. The degree of stringency can be controlled by temperature, ionic strength, pH and the presence of a partially denaturing solvent such as formamide.

[0059] Typically, stringent hybridization conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30.degree. C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60.degree. C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide.

[0060] Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulfate) at 37.degree. C., and a wash in 1.times. to 2.times.SSC (20.times.SSC=3.0 M NaCl/0.3 M trisodium citrate) at 50.degree. C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 M NaCl, 1% SDS at 37.degree. C., and a wash in 0.5.times. to 1.times.SSC at 55.degree. C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37.degree. C., and a wash in 0.1.times.SSC at 60.degree. C. Typically the time of hybridization is from 4 to 16 hours.

[0061] An extensive guide to the hybridization of nucleic acids is found in Tijssen, Laboratory Techniques in Biochemistry and Molecular Biology--Hybridization with Nucleic Acid Probes, Part I, Chapter 2 "Overview of principles of hybridization and the strategy of nucleic acid probe assays", Elsevier, New York (1993); and Current Protocols in Molecular Biology, Chapter 2, Ausubel et al., Eds., Greene Publishing and Wiley-Interscience, New York (1995). Often, cDNA libraries will be normalized to increase the representation of relatively rare cDNAs.

[0062] The nucleic acids of the invention can be amplified from nucleic acid samples using amplification techniques. For instance, polymerase chain reaction (PCR) technology can be used to amplify the sequences of polynucleotides of the present invention and related polynucleotides directly from genomic DNA or cDNA libraries. PCR and other in vitro amplification methods may also be useful, for example, to clone nucleic acid sequences that code for proteins to be expressed, to make nucleic acids to use as probes for detecting the presence of the desired mRNA in samples, for nucleic acid sequencing, or for other purposes.

[0063] Examples of techniques useful for in vitro amplification methods are found in Berger, Sambrook, and Ausubel, as well as Mullis et al., U.S. Pat. No. 4,683,202 (1987); and, PCR Protocols A Guide to Methods and Applications, Innis et al., Eds., Academic Press Inc., San Diego, Calif. (1990). Commercially available kits for genomic PCR amplification are known in the art. See, e.g., Advantage-GC Genomic PCR Kit (Clontech). The T4 gene 32 protein (Boehringer Mannheim) can be used to improve yield of long PCR products. PCR-based screening methods have also been described. Wilfinger et al. describe a PCR-based method in which the longest cDNA is identified in the first step so that incomplete clones can be eliminated from study. BioTechniques, 22(3):481-486 (1997).

[0064] In one aspect of the invention, nucleic acids can be amplified from a plant nucleic acid library. The nucleic acid library may be a cDNA library, a genomic library, or a library generally constructed from nuclear transcripts at any stage of intron processing. Libraries can be made from a variety of plant tissues such as ears, seedlings, leaves, stalks, roots, pollen, or seeds. Good results have been obtained using tissues such as corn nucellus 5 days after silking, corn embryos 20 days after pollination, and dissected embryo sacs at 4, 5, and 6, days after pollination.

[0065] Alternatively, the sequences of the invention can be used to isolate corresponding sequences in other organisms, particularly other plants, more particularly, other monocots. In this manner, methods such as PCR, hybridization, and the like can be used to identify such sequences having substantial sequence similarity to the sequences of the invention. See, for example, Sambrook et al., (1989) Molecular Cloning: A Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.). and Innis et al. (1990), PCR Protocols: A Guide to Methods and Applications (Academic Press, New York). Coding sequences isolated based on their sequence identity to the entire inventive coding sequences set forth herein or to fragments thereof are encompassed by the present invention.

[0066] The isolated nucleic acids of the present invention can also be prepared by direct chemical synthesis by methods such as the phosphotriester method of Narang et al., Meth. Enzymol. 68:90-99 (1979); the phosphodiester method of Brown et al., Meth. Enzymol. 68:109-151 (1979); the diethylphosphoramidite method of Beaucage et al., Tetra. Lett. 22:1859-1862 (1981); the solid phase phosphoramidite triester method described by Beaucage and Caruthers, Tetra. Letts. 22(20):1859-1862 (1981), e.g., using an automated synthesizer, e.g., as described in Needham-VanDevanter et al., Nucleic Acids Res. 12:6159-6168 (1984); and, the solid support method of U.S. Pat. No. 4,458,066. Chemical synthesis generally produces a single stranded oligonucleotide. This may be converted into double stranded DNA by hybridization with a complementary sequence, or by polymerization with a DNA polymerase using the single strand as a template. One of skill will recognize that while chemical synthesis of DNA is limited to sequences of about 100 bases, longer sequences may be obtained by the ligation of shorter sequences.

Regulatory Sequences

[0067] Transformation of organisms with exogenous DNA sequences can provide commercially desirable strains. In the case of plants, transformation can lead to new varieties having heritable traits of insect resistance, herbicide resistance, and other agronomically important nutritional or morphological properties.

[0068] Diverse regulatory sequences are needed as undesirable biochemical interactions result from using the same regulatory sequence to control more than one gene. For example, transformation with multiple copies of a regulatory element may cause homologous recombination between two or more expression systems, formation of hairpin loops caused from two copies of the same promoter or enhancer in opposite orientation in close proximity, competition between identical expression systems for binding to common promoter-specific regulatory factors, and inappropriate expression levels of an exogenous gene due to trans effects of a second promoter or enhancer.

[0069] The limited number of known regulatory sequences does not cover the desired range of conditions for transgenic expression. Accordingly, any new sequence having a regulatory role in promoting, enhancing or otherwise regulating gene expression could be used in new combinations with known regulatory sequences and would be a valuable contribution to the art.

[0070] By "promoter" or "transcriptional initiation region" is intended a regulatory region of DNA usually comprising a TATA box capable of directing RNA polymerase 11 to initiate RNA synthesis at the appropriate transcription initiation site for a particular coding sequence. A promoter may additionally comprise other recognition sequences generally positioned upstream or 5' to the TATA box, referred to as upstream promoter elements, which influence the transcription initiation rate. It is recognized that having identified the nucleotide sequences for the promoter regions disclosed herein, it is within the state of the art to isolate and identify further regulatory elements in the 5' untranslated region upstream from the particular promoter regions identified herein. Thus the promoter regions disclosed herein are generally further defined by comprising upstream regulatory elements such as those responsible for tissue and temporal expression of the coding sequence, enhancers and the like. In the same manner, the promoter elements which enable expression in the desired tissue such as the seed can be identified, isolated, and used with other core promoters to confirm seed-preferred expression.

[0071] It is recognized that the promoters may be used with their native coding sequences to increase or decrease expression resulting in a change in phenotype in the transformed plant.

[0072] A regulatory sequence "variant" is a modified form of a regulatory sequence wherein one or more bases have been modified, removed or added. For example, a routine way to remove part of a DNA sequence is to use an exonuclease in combination with DNA amplification to produce unidirectional nested deletions of double stranded DNA clones. A commercial kit for this purpose is sold under the trade name Exo-Size.TM. (New England Biolabs, Beverly, Mass.). Briefly, this procedure entails incubating exonuclease III with DNA to remove progressively nucleotides in the 3' to 5' direction at 5' overhangs, blunt ends or nicks in the DNA template. However, exonuclease III is unable to remove nucleotides at 3',4-base overhangs. Timed digests of a clone with this enzyme produces unidirectional nested deletions. After digestion, mung bean nuclease removes single stranded DNA overhangs and the blunt ends are ligated with T4 DNA ligase.

[0073] One example of a regulatory sequence variant is a promoter formed by one or more deletions from a larger promoter. The 5' portion of a promoter up to the TATA box near the transcription start site can be deleted without abolishing promoter activity, as described by Zhu et al., The Plant Cell 7:1681-89 (1995).

[0074] Those fragments of promoter nucleotide sequences disclosed herein that operate to promote the seed-preferred expression of an operably linked heterologous nucleotide sequence, are referred to as "functional fragments". These fragments will comprise at least about 20 contiguous nucleotides, preferably at least about 50 contiguous nucleotides, more preferably at least about 75 contiguous nucleotides, even more preferably at least about 100 contiguous nucleotides of the particular promoter nucleotide sequence disclosed herein. The nucleotides of such fragments will usually comprise the TATA recognition sequence of the particular promoter sequence. Such fragments may be obtained by use of restriction enzymes to cleave the naturally occurring promoter nucleotide sequences disclosed herein; by synthesizing a nucleotide sequence from the naturally occurring promoter DNA sequence; or may be obtained through the use of PCR technology. See particularly, Mullis et al. (1987) Methods Enzymol. 155:335-350, and Erlich, ed. (1989) PCR Technology (Stockton Press, New York). Again, variants of these promoter fragments, such as those resulting from site-directed mutagenesis, are encompassed by the compositions of the present invention.

[0075] An artisan can modify the regulatory sequences as summarized above and expect to obtain a functional product. Others have made these kinds of simple modifications resulting in functional fragments. For example, Rieping and Schoffl reported that "[c]omplete reconstruction of a native (heat shock) promoter region increased" transgenic expression "only very little, but deletion of CCAAT box sequences reduced CAT expression five-fold." Mol. Gen. Genet. 231:226-32 (1992). The same authors also reported using a "series of deletion mutants of a soybean heat shock (hs) promoter" and that heat inducible "activities were detected except in plants containing a transcriptional fusion devoid of all but 18 nucleotides at the 5' terminus of the hs gene transcript." Mol. Gen. Genet. 217:246-53 (1989).

[0076] The coding sequence expressed by the promoters of the invention may be used for varying the phenotype of the seeds. Various changes in phenotype are of interest including modifying the fatty acid composition in seeds, altering the starch or carbohydrate profile, altering the amino acid content of the seed, and the like. These results can be achieved by providing expression of heterologous or increased expression of endogenous products in seeds. Alternatively, the results can be achieved by providing for a reduction of expression of one or more endogenous products, particularly enzymes or cofactors in the seed. These changes result in a change in phenotype of the transformed seed.

[0077] A wide variety of polypeptides or RNA, such as an antisense sequence, can be expressed transgenically under the control of an inventive regulatory sequence. In practice, DNA that codes for a desired polypeptide or RNA is fused to a promoter to achieve a high level of expression. In many cases, the controlled polypeptide or RNA may not be native to the organism in which it is expressed. DNA coding for the polypeptide may be modified to reflect preferred codon usage in the particular species that is the transformation target.

[0078] In cases where the controlled gene is to be expressed in a plant seed or tissue, especially desired are regulatory elements of a "seed tissue-preferred" or "seed tissue-specific" promoter, that is, a promoter that drives high expression of the heterologous DNA segment in seed tissue where control of genes involved in seed metabolism is desired, and little or no expression in other parts of the plant. Manufacture of the protein encoded by the heterologous DNA segment in other parts of the plant needlessly expends the plant's energy.

[0079] A regulatory sequence of the invention is advantageously combined with a selectable marker gene, in physical proximity to the introduced DNA segment. The marker gene allows recovery of transformed cells by positive genetic selection or screening. The selectable marker gene also provides continuing selection pressure on a transgenic plant population, to ensure that the introduced DNA segment is retained by the transgenic organism.

[0080] Many commonly used positive selectable marker genes for transformation have been isolated from bacteria and code for enzymes that metabolically detoxify a selective chemical agent which may be an antibiotic or a herbicide. Other positive selection marker genes encode an altered target which is insensitive to the inhibitor.

[0081] In order to create an expression vector containing the gene and a regulatory sequence to control the gene, an expression cassette first is made by inserting a cloned gene, or a DNA segment comprising the desired regulatory sequences fused to a DNA sequence encoding a desired high-value protein as described above, into a plasmid.

[0082] One or more copies of the expression cassette containing the introduced DNA is transferred to an expression vector. In a preferred embodiment, the vector also contains a gene encoding a selection marker which functionally is linked to a promoter regulatory sequence that controls transcription initiation.

[0083] Transformation protocols as well as protocols for introducing nucleotide sequences into plants may vary depending on the type of plant or plant cell, i.e., monocot or dicot, targeted for transformation as described further herein.

Expression Cassettes

[0084] Expression cassettes comprising isolated nucleic acids of the present invention are provided. An expression cassette will typically comprise a polynucleotide of the present invention operably linked to transcriptional initiation regulatory sequences which will direct the transcription of the polynucleotide in the intended host cell, such as tissues of a transformed plant.

[0085] The construction of such expression cassettes which can be employed in conjunction with the present invention is well known to those of skill in the art in light of the present disclosure. See, e.g., Sambrook et al.; Molecular Cloning: A Laboratory Manual; Cold Spring Harbor, New York; (1989); Gelvin et al.; Plant Molecular Biology Manual (1990); Plant Biotechnology: Commercial Prospects and Problems, eds. Prakash et al.; Oxford & IBH Publishing Co.; New Delhi, India; (1993); and Heslot et al.; Molecular Biology and Genetic Engineering of Yeasts; CRC Press, Inc., USA; (1992); each incorporated herein in its entirety by reference.

[0086] For example, plant expression vectors may include one or more cloned plant genes under the transcriptional control of 5' and 3' regulatory sequences and a dominant selectable marker. Such plant expression vectors may also contain, if desired, a promoter regulatory region (e.g., one conferring inducible, constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific/selective expression), a transcription initiation start site, a ribosome binding site, an RNA processing signal, a transcription termination site, and/or a polyadenylation signal.

[0087] Constitutive, tissue-preferred or inducible promoters can be employed. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcription initiation region, the 1'- or 2'-promoter derived from T-DNA of Agrobacterium tumefaciens, the actin promoter, the ubiquitin promoter, the histone H2B promoter (Nakayama et al., 1992, FEBS Lett 30:167-170), the Smas promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), the Nos promoter, the pEmu promoter, the rubisco promoter, the GRP1-8 promoter, and other transcription initiation regions from various plant genes known in the art.

[0088] Examples of inducible promoters are the Adh1 promoter which is inducible by hypoxia or cold stress, the Hsp70 promoter which is inducible by heat stress, the PPDK promoter which is inducible by light, the In2 promoter which is safener induced, the ERE promoter which is estrogen induced and the Pepcarboxylase promoter which is light induced.

[0089] Examples of promoters under developmental control include promoters that initiate transcription preferentially in certain tissues, such as leaves, roots, fruit, pollen, seeds, or flowers. An exemplary promoter is the anther specific promoter 5126 (U.S. Pat. Nos. 5,689,049 and 5,689,051). Examples of seed-preferred promoters include, but are not limited to, 22 kDa zein promoter, 27 kD gamma zein promoter and waxy promoter, (Boronat, A., et al., Plant Sci. 47:95-102 (1986); Reina, M., et al., Nucleic Acids Res. 18(21):6426 (1990); Kloesgen, R. B., et al., Mol. Gen. Genet. 203:237-244 (1986)), as well as the globulin 1, oleosin and the phaseolin promoters. A typical aleurone-preferred promoter is the Ltp2 promoter (Kalla, R., et al., Plant Journal, 4:849-860,1994; see also SEQ ID NO:18). The barley nuc1 promoter (see SEQ ID NO:17), and cim1 promoter (U.S. Pat. No. 6,225,529 issued May 1, 2001) are examples of nucellus tissue preferred promoters. Developing endosperm promoters that may be used are the end1 and end2 promoters (see SEQ ID NOS:19 and 20). The disclosures each of these are incorporated herein by reference in their entirety.

[0090] Either heterologous or non-heterologous (i.e., endogenous) promoters can be employed to direct expression of the nucleic acids of the present invention. These promoters can also be used, for example, in expression cassettes to drive expression of antisense nucleic acids to reduce, increase, or alter concentration and/or composition of the proteins of the present invention in a desired tissue.

[0091] If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3'-end of a polynucleotide coding region. The polyadenylation region can be derived from the natural gene, from a variety of other plant genes, or from T-DNA. The 3' end sequence to be added can be derived from, for example, the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

[0092] An intron sequence can be added to the 5' untranslated region or the coding sequence of the partial coding sequence to increase the amount of the mature message that accumulates. See for example Buchman and Berg, Mol. Cell Biol. 8:4395-4405 (1988); Callis et al., Genes Dev. 1:1183-1200 (1987). Use of maize introns Adh1-S intron 1, 2, and 6, the Bronze-1 intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, New York (1994).

[0093] The vector comprising the sequences from a polynucleotide of the present invention will typically comprise a marker gene which confers a selectable phenotype on plant cells. Usually, the selectable marker gene encodes antibiotic or herbicide resistance. Suitable genes include those coding for resistance to the antibiotics spectinomycin and streptomycin (e.g., the aada gene), the streptomycin phosphotransferase (SPT) gene coding for streptomycin resistance, the neomycin phosphotransferase (NPTII) gene encoding kanamycin or geneticin resistance, the hygromycin phosphotransferase (HPT) gene coding for hygromycin resistance.

[0094] Suitable genes coding for resistance to herbicides include those which act to inhibit the action of acetolactate synthase (ALS), in particular the sulfonylurea-type herbicides (e.g., the acetolactate synthase (ALS) gene containing mutations leading to such resistance in particular the S4 and/or Hra mutations), those which act to inhibit action of glutamine synthase, such as phosphinothricin or basta (e.g., the bar gene), or other such genes known in the art. The bar gene encodes resistance to the herbicide basta and the ALS gene encodes resistance to the herbicide chlorsulfuron.

[0095] Typical vectors useful for expression of genes in higher plants are well known in the art and include vectors derived from the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens described by Rogers et al., Meth. In Enzymol. 153:253-277 (1987). Exemplary A. tumefaciens vectors useful herein are plasmids pKYLX6 and pKYLX7 of Schardl et al., Gene 61:1-11 (1987) and Berger et al., Proc. Natl. Acad. Sci. USA 86:8402-8406 (1989). Another useful vector herein is plasmid pBI101.2 that is available from Clontech Laboratories, Inc. (Palo Alto, Calif.).

[0096] A variety of plant viruses that can be employed as vectors are known in the art and include cauliflower mosaic virus (CaMV), geminivirus, brome mosaic virus, and tobacco mosaic virus.

[0097] A polynucleotide of the present invention can be expressed in either sense or anti-sense orientation as desired. In plant cells, it has been shown that antisense RNA inhibits gene expression by preventing the accumulation of mRNA which encodes the enzyme of interest, see, e.g., Sheehy et al., Proc. Natl. Acad. Sci. USA 85:8805-8809 (1988); and Hiatt et al., U.S. Pat. No. 4,801,340.

[0098] Another method of suppression is sense suppression. Introduction of nucleic acid configured in the sense orientation has been shown to be an effective means by which to block the transcription of target genes. For an example of the use of this method to modulate expression of endogenous genes see, Napoli et al., The Plant Cell 2:279-289 (1990) and U.S. Pat. No. 5,034,323.

[0099] Recent work has shown suppression with the use of double stranded RNA. Such work is described in Tabara et al., Science 282:5388:430-431 (1998), WO 99/53050 and WO 98/53083.

[0100] Catalytic RNA molecules or ribozymes can also be used to inhibit expression of plant genes. The inclusion of ribozyme sequences within antisense RNAs confers RNA-cleaving activity upon them, thereby increasing the activity of the constructs. The design and use of target RNA-specific ribozymes is described in Haseloff et al., Nature 334:585-591 (1988).

[0101] A variety of cross-linking agents, alkylating agents and radical generating species as pendant groups on polynucleotides of the present invention can be used to bind, label, detect, and/or cleave nucleic acids. For example, Vlassov, V. V., et al., Nucleic Acids Res (1986) 14:4065-4076, describe covalent bonding of a single-stranded DNA fragment with alkylating derivatives of nucleotides complementary to target sequences. A report of similar work by the same group is that by Knorre, D. G., et al., Biochimie (1985) 67:785-789. Iverson and Dervan also showed sequence-specific cleavage of single-stranded DNA mediated by incorporation of a modified nucleotide which was capable of activating cleavage (J. Am. Chem. Soc. (1987) 109:1241-1243). Meyer, R. B., et al., J. Am. Chem. Soc. (1989) 111:8517-8519, effect covalent crosslinking to a target nucleotide using an alkylating agent complementary to the single-stranded target nucleotide sequence. A photoactivated crosslinking to single-stranded oligonucleotides mediated by psoralen was disclosed by Lee, B. L., et al., Biochemistry (1988) 27:3197-3203. Use of crosslinking in triple-helix forming probes was also disclosed by Home et al., J. Am. Chem. Soc. (1990) 112:2435-2437. Use of N4, N4-ethanocytosine as an alkylating agent to crosslink to single-stranded oligonucleotides has also been described by Webb and Matteucci, J. Am. Chem. Soc. (1986) 108:2764-2765; Nucleic Acids Res (1986) 14:7661-7674; Feteritz et al., J. Am. Chem. Soc. 113:4000 (1991). Various compounds to bind, detect, label, and/or cleave nucleic acids are known in the art. See, for example, U.S. Pat. Nos. 5,543,507; 5,672,593; 5,484,908; 5,256,648; and, 5,681941.

Proteins

[0102] Proteins of the present invention include proteins having the disclosed sequences as well proteins coded by the disclosed polynucleotides. In addition, proteins of the present invention include proteins derived from the native protein by deletion, addition or substitution of one or more amino acids at one or more sites in the native protein. Such variants may result from, for example, genetic polymorphism or from human manipulation. Methods for such manipulations are generally known in the art.

[0103] For example, amino acid sequence variants of the polypeptide can be prepared by mutations in the cloned DNA sequence encoding the native protein of interest. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Walker and Gaastra, eds. (1983) Techniques in Molecular Biology (MacMillan Publishing Company, New York); Kunkel (1985) Proc. Natl. Acad. Sci. USA 82:488-492; Kunkel et al. (1987) Methods Enzymol. 154:367-382; Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, N.Y.); U.S. Pat. No. 4,873,192; and the references cited therein; herein incorporated by reference. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al. (1978) Atlas of Protein Sequence and Structure (Natl. Biomed. Res. Found., Washington, D.C.), herein incorporated by reference. Conservative substitutions, such as exchanging one amino acid with another having similar properties, may be preferred.

[0104] In constructing variants of the proteins of interest, modifications to the nucleotide sequences encoding the variants can generally be made such that variants continue to possess the desired activity.

[0105] The isolated proteins of the present invention include a polypeptide comprising at least 25 contiguous amino acids encoded by any one of the nucleic acids of the present invention, or polypeptides that are conservatively modified variants thereof. The proteins of the present invention or variants thereof can comprise any number of contiguous amino acid residues from a polypeptide of the present invention, wherein that number is selected from the group of integers consisting of from 25 to the number of residues in a full-length polypeptide of the present invention. Optionally, this subsequence of contiguous amino acids is at least 25, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, or 500 amino acids in length.

[0106] The present invention includes catalytically active polypeptides (i.e., enzymes). Catalytically active polypeptides will generally have a specific activity of at least about 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% that of the native (non-synthetic), endogenous polypeptide. Further, the substrate specificity (k.sub.cat/K.sub.m) is optionally substantially similar to the native (non-synthetic), endogenous polypeptide. Typically, the Km will be at least about 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% that of the native (non-synthetic), endogenous polypeptide. Methods of assaying and quantifying measures of enzymatic activity and substrate specificity (k.sub.cat/K.sub.m), are well known to those of skill in the art. See, e.g., Segel, Biochemical Calculations, 2.sup.nd ed., John Wiley and Sons, New York (1976).

[0107] The present invention includes modifications that can be made to an inventive protein. In particular, it may be desirable to diminish the activity of the gene. Other modifications may be made to facilitate the cloning, expression, or incorporation of the targeting molecule into a fusion protein. Such modifications are well known to those of skill in the art and include, for example, a methionine added at the amino terminus to provide an initiation site, or additional amino acids (e.g., poly His) placed on either terminus to create conveniently located restriction sites or termination codons or purification sequences.

[0108] Using the nucleic acids of the present invention, one may express a protein of the present invention in recombinantly engineered cells such as bacteria, yeast, insect, mammalian, or plant cells. The cells produce the protein in a non-natural condition (e.g., in quantity, composition, location, and/or time), because they have been genetically altered through human intervention to do so.

[0109] Typically, an intermediate host cell may be used in the practice of this invention to increase the copy number of the cloning vector. With an increased copy number, the vector containing the gene of interest can be isolated in significant quantities for introduction into the desired plant cells.

[0110] Host cells that can be used in the practice of this invention include prokaryotes and eukaryotes. Prokaryotes include bacterial hosts such as Eschericia coli, Salmonella typhimurium, and Serratia marcescens. Eukaryotic hosts such as yeast, insect cells or filamentous fungi may also be used in this invention.

[0111] Commonly used prokaryotic control sequences include such commonly used promoters as the beta lactamase (penicillinase) and lactose (lac) promoter systems (Chang et al., Nature 198:1056 (1977)), the tryptophan (trp) promoter system (Goeddel et al., Nucleic Acids Res. 8:4057 (1980)) and the lambda derived P L promoter and N-gene ribosome binding site (Shimatake et al., Nature 292:128 (1981)). The inclusion of selection markers in DNA vectors transfected in E. coli is also useful. Examples of such markers include genes specifying resistance to ampicillin, tetracycline, or chloramphenicol.

[0112] The vector is selected to allow introduction into the appropriate host cell. Bacterial vectors are typically of plasmid or phage origin. Expression systems for expressing a protein of the present invention are available using Bacillus sp. and Salmonella (Palva et al., Gene 22:229-235 (1983); Mosbach et al., Nature 302:543-545 (1983)).

[0113] Synthesis of heterologous proteins in yeast is well known. See Sherman, F., et al., Methods in Yeast Genetics, Cold Spring Harbor Laboratory (1982). Two widely utilized yeast for production of eukaryotic proteins are Saccharomyces cerevisiae and Pichia pastoris. Vectors, strains, and protocols for expression in Saccharomyces and Pichia are known in the art and available from commercial suppliers (e.g., Invitrogen). Suitable vectors usually have expression control sequences, such as promoters, including 3-phosphoglycerate kinase or alcohol oxidase, and an origin of replication, termination sequences and the like as desired.

[0114] The baculovirus expression system (BES) is a eukaryotic, helper-independent expression system which has been used to express hundreds of foreign genes (Luckow, V., Ch. 4 "Cloning and Expression of Heterologous Genes in Insect Cells with Baculovirus Vectors" in Recombinant DNA Technology and Applications, A. Prokop et al., Eds. McGraw-Hill, Inc. (1991); Luckow, V., Ch. 10 "Insect Expression Technology" in Principles & Practice of Protein Engineering, J. L. Cleland and C. S. Craig, Eds. John Wiley & Sons, (1994)).

[0115] Recombinant baculoviruses are generated by inserting the particular gene- or genes-of-interest into the baculovirus genome using established protocols with vectors and reagents from commercial suppliers (e.g., Invitrogen, Life Technologies Incorporated). Commercial vectors are readily available with various promoters, such as polyhedrin and p10, optional signal sequences for protein secretion, or affinity tags, such as 6.times. histidine. These recombinant viruses are grown, maintained and propagated in commercially available cell lines derived from several insect species including Spodoptera frugiperda and Trichoplusia ni. The insect cells can be cultured using well-established protocols in a variety of different media, for example, with and without bovine serum supplementation. The cultured cells are infected with the recombinant viruses and the gene-of-interest polypeptide is expressed. Proteins expressed with the baculovirus system have been extensively characterized and, in many cases, their post-translational modifications such as phosphorylation, acylation, etc., are identical to the natively expressed protein.

[0116] A protein of the present invention, once expressed, can be isolated from cells by lysing the cells and applying standard protein isolation techniques to the lysates. The monitoring of the purification process can be accomplished by using Western blot techniques or radioimmunoassay or other standard immunoassay techniques. Expression cassettes are also available which direct the expressed protein to be secreted from the cell into the media. In these cases, the expressed protein can be purified from the cell growth media using standard protein purification techniques.

[0117] The proteins of the present invention can also be constructed using non-cellular synthetic methods. Solid phase synthesis of proteins of less than about 50 amino acids in length may be accomplished by attaching the C-terminal amino acid of the sequence to an insoluble support followed by sequential addition of the remaining amino acids in the sequence. Techniques for solid phase synthesis are described by Barany and Merrifield, Solid-Phase Peptide Synthesis, pp. 3-284 in The Peptides: Analysis, Synthesis, Biology. Vol. 2: Special Methods in Peptide Synthesis, Part A.; Merrifield et al., J. Am. Chem. Soc. 85:2149-2156 (1963), and Stewartet al., Solid Phase Peptide Synthesis, 2nd ed., Pierce Chem. Co., Rockford, Ill. (1984). Proteins of greater length may be synthesized by condensation of the amino and carboxy termini of shorter fragments. Methods of forming peptide bonds by activation of a carboxy terminal end (e.g., by the use of the coupling reagent N,N'-dicyclohexylcarbodiimide)) are known to those of skill.

[0118] The proteins of this invention, recombinant or synthetic, may be purified to substantial purity by standard techniques well known in the art, including detergent solubilization, selective precipitation with such substances as ammonium sulfate, column chromatography, immunopurification methods, and others. See, for instance, R. Scopes, Protein Purification: Principles and Practice, Springer-Verlag: New York (1982); Deutscher, Guide to Protein Purification, Academic Press (1990). For example, antibodies may be raised to the proteins as described herein. Purification from E. coli can be achieved following procedures described in U.S. Pat. No. 4,511,503. Detection of the expressed protein is achieved by methods known in the art and include, for example, radioimmunoassays, Western blotting techniques or immunoprecipitation.

[0119] The present invention further provides a method for modulating (i.e., increasing or decreasing) the concentration or composition of the polypeptides of the present invention in a plant or part thereof. Modulation can be effected by increasing or decreasing the concentration and/or the composition (i.e., the ratio of the polypeptides of the present invention) in a plant.

[0120] The method comprises transforming a plant cell with an expression cassette comprising a polynucleotide of the present invention to obtain a transformed plant cell, growing the transformed plant cell under conditions allowing expression of the polynucleotide in the plant cell in an amount sufficient to modulate concentration and/or composition in the plant cell.

[0121] In some embodiments, the content and/or composition of polypeptides of the present invention in a plant may be modulated by altering, in vivo or in vitro, the promoter of a non-isolated gene of the present invention to up- or down-regulate gene expression. In some embodiments, the coding regions of native genes of the present invention can be altered via substitution, addition, insertion, or deletion to decrease activity of the encoded enzyme. See, e.g., Kmiec, U.S. Pat. No. 5,565,350; Zarling et al., PCT/US93/03868. One method of down-regulation of the protein involves using PEST sequences that provide a target for degradation of the protein.

[0122] In some embodiments, an isolated nucleic acid (e.g., a vector) comprising a promoter sequence is transfected into a plant cell. Subsequently, a plant cell comprising the promoter operably linked to a polynucleotide of the present invention is selected for by means known to those of skill in the art such as, but not limited to, Southern blot, DNA sequencing, or PCR analysis using primers specific to the promoter and to the gene and detecting amplicons produced therefrom. A plant or plant part altered or modified by the foregoing embodiments is grown under plant growing conditions for a time sufficient to: alter the aleurone cells in the seeds of the plant, to increase the level of oil in the seeds of the plant, or to otherwise improve the grain characteristics of the seed of the plant. Plant growing conditions are well known in the art.

[0123] In general, content of the polypeptide is increased or decreased by at least 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, or 90% relative to a native control plant, plant part, or cell lacking the aforementioned expression cassette. Modulation in the present invention may occur during and/or subsequent to growth of the plant to the desired stage of development. Modulating nucleic acid expression temporally and/or in particular tissues can be controlled by employing the appropriate promoter operably linked to a polynucleotide of the present invention in, for example, sense or antisense orientation as discussed in greater detail, supra. Induction of expression of a polynucleotide of the present invention can also be controlled by exogenous administration of an effective amount of inducing compound. Inducible promoters and inducing compounds which activate expression from these promoters are well known in the art. In certain embodiments, the polypeptides of the present invention are modulated in monocots or dicots, for example: maize, wheat, rice, barley, soybean, arabidopsis, oats, sorghum, millet, rye, sunflower, safflower, alfalfa, canola, or cotton.

[0124] Means of detecting the proteins of the present invention are not critical aspects of the present invention. The proteins can be detected and/or quantified using any of a number of well-recognized immunological binding assays (see, e.g., U.S. Pat. Nos. 4,366,241; 4,376,110; 4,517,288; and 4,837,168). For a review of the general immunoassays, see also Methods in Cell Biology, Vol. 37: Antibodies in Cell Biology, Asai, Ed., Academic Press, Inc. New York (1993); Basic and Clinical Immunology 7th Edition, Stites & Terr, Eds. (1991). Moreover, the immunoassays of the present invention can be performed in any of several configurations, e.g., those reviewed in Enzyme Immunoassay, Maggio, Ed., CRC Press, Boca Raton, Fla. (1980); Tijan, Practice and Theory of Enzyme Immunoassays, Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers B. V., Amsterdam (1985); Harlow and Lane, supra; Immunoassay: A Practical Guide, Chan, Ed., Academic Press, Orlando, Fla. (1987); Principles and Practice of Immunoassays, Price and Newman Eds., Stockton Press, NY (1991); and Non-isotopic Immunoassays, Ngo, Ed., Plenum Press, NY (1988).

[0125] Typical methods include Western blot (immunoblot) analysis, analytic biochemical methods such as electrophoresis, capillary electrophoresis, high performance liquid chromatography (HPLC), thin layer chromatography (TLC), hyperdiffusion chromatography, and the like, and various immunological methods such as fluid or gel precipitin reactions, immunodiffusion (single or double), immunoelectrophoresis, radioimmunoassays (RIAs), enzyme-linked immunosorbent assays (ELISAs), immunofluorescent assays, and the like.

[0126] Non-radioactive labels are often attached by indirect means. Generally, a ligand molecule (e.g., biotin) is covalently bound to the molecule. The ligand then binds to an anti-ligand (e.g., streptavidin) molecule which is either inherently detectable or covalently bound to a signal system, such as a detectable enzyme, a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands can be used. Where a ligand has a natural anti-ligand, for example, biotin, thyroxine, and cortisol, it can be used in conjunction with the labeled, naturally occurring anti-ligands. Alternatively, any haptenic or antigenic compound can be used in combination with an antibody.

[0127] The molecules can also be conjugated directly to signal generating compounds, e.g., by conjugation with an enzyme or fluorophore. Enzymes of interest as labels will primarily be hydrolases, particularly phosphatases, esterases and glycosidases, or oxidoreductases, particularly peroxidases. Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, etc. Chemiluminescent compounds include luciferin, and 2,3-dihydrophthalazinediones, e.g., luminol. For a review of various labeling or signal producing systems which may be used, see, U.S. Pat. No. 4,391,904, which is incorporated herein by reference.

[0128] Some assay formats do not require the use of labeled components. For instance, agglutination assays can be used to detect the presence of the target antibodies. In this case, antigen-coated particles are agglutinated by samples comprising the target antibodies. In this format, none of the components need be labeled and the presence of the target antibody is detected by simple visual inspection.

[0129] The proteins of the present invention can be used for identifying compounds that bind to (e.g., substrates), and/or increase or decrease (i.e., modulate) the enzymatic activity of catalytically active polypeptides of the present invention. The method comprises contacting a polypeptide of the present invention with a compound whose ability to bind to or modulate enzyme activity is to be determined. The polypeptide employed will have at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95% of the specific activity of the native, full-length polypeptide of the present invention (e.g., enzyme). Methods of measuring enzyme kinetics are well known in the art. See, e.g., Segel, Biochemical Calculations, 2.sup.nd ed., John Wiley and Sons, New York (1976).

[0130] Antibodies can be raised to a protein of the present invention, including individual, allelic, strain, or species variants, and fragments thereof, both in their naturally occurring (full-length) forms and in recombinant forms. Additionally, antibodies are raised to these proteins in either their native configurations or in non-native configurations. Anti-idiotypic antibodies can also be generated. Many methods of making antibodies are known to persons of skill.

[0131] In some instances, it is desirable to prepare monoclonal antibodies from various mammalian hosts, such as mice, rodents, primates, humans, etc. Description of techniques for preparing such monoclonal antibodies are found in, e.g., Basic and Clinical Immunology, 4th ed., Stites et al., Eds., Lange Medical Publications, Los Altos, Calif. and references cited therein; Harlow and Lane, Supra; Goding, Monoclonal Antibodies: Principles and Practice, 2nd ed., Academic Press, New York, N.Y. (1986); and Kohler and Milstein, Nature 256:495-497 (1975).

[0132] Other suitable techniques involve selection of libraries of recombinant antibodies in phage or similar vectors (see, e.g., Huse et al., Science 246:1275-1281 (1989); and Ward et al., Nature 341:544-546 (1989); and Vaughan et al., Nature Biotechnology 14:309-314 (1996)). Alternatively, high avidity human monoclonal antibodies can be obtained from transgenic mice comprising fragments of the unrearranged human heavy and light chain Ig loci (i.e., minilocus transgenic mice). Fishwild et al., Nature Biotech. 14:845-851 (1996). Also, recombinant immunoglobulins may be produced. See, Cabilly, U.S. Pat. No. 4,816,567; and Queen et al., Proc. Natl. Acad. Sci. U.S.A. 86:10029-10033 (1989).

[0133] The antibodies of this invention can be used for affinity chromatography in isolating proteins of the present invention, for screening expression libraries for particular expression products such as normal or abnormal protein or for raising anti-idiotypic antibodies which are useful for detecting or diagnosing various pathological conditions related to the presence of the respective antigens.

[0134] Frequently, the proteins and antibodies of the present invention may be labeled by joining, either covalently or non-covalently, a substance which provides for a detectable signal. A wide variety of labels and conjugation techniques are known and are reported extensively in both the scientific and patent literature. Suitable labels include radionucleotides, enzymes, substrates, cofactors, inhibitors, fluorescent moieties, chemiluminescent moieties, magnetic particles, and the like.

Transformation of Cells

[0135] The method of transformation is not critical to the present invention; various methods of transformation are currently available. As newer methods are available to transform crops or other host cells they may be directly applied. Accordingly, a wide variety of methods have been developed to insert a DNA sequence into the genome of a host cell to obtain the transcription and/or translation of the sequence to effect phenotypic changes in the organism. Thus, any method which provides for efficient transformation/transfection may be employed.

[0136] A DNA sequence coding for the desired polynucleotide of the present invention, for example a cDNA or a genomic sequence encoding a full length protein or functional portion thereof, can be used to construct an expression cassette which can be introduced into the desired plant. Isolated nucleic acid acids of the present invention can be introduced into plants according to techniques known in the art. Generally, expression cassettes as described above and suitable for transformation of plant cells are prepared.

[0137] Techniques for transforming a wide variety of higher plant species are well known and described in the technical, scientific, and patent literature. See, for example, Weising et al., Ann. Rev. Genet. 22:421-477 (1988). For example, the DNA construct may be introduced directly into the genomic DNA of the plant cell using techniques such as electroporation, PEG poration, particle bombardment, silicon fiber delivery, or microinjection of plant cell protoplasts or embryogenic callus. See, e.g., Tomes et al., Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment. pp.197-213 in Plant Cell, Tissue and Organ Culture, Fundamental Methods, Eds. O. L. Gamborg and G. C. Phillips, Springer-Verlag Berlin Heidelberg New York, 1995. Alternatively, the DNA constructs may be combined with suitable T-DNA flanking regions and introduced into a conventional Agrobacterium tumefaciens host vector. The virulence functions of the Agrobacterium tumefaciens host will direct the insertion of the construct and adjacent marker into the plant cell DNA when the cell is infected by the bacteria. See, U.S. Pat. No. 5,591,616.

[0138] The introduction of DNA constructs using polyethylene glycol precipitation is described in Paszkowski et al., Embo J. 3:2717-2722 (1984). Electroporation techniques are described in Fromm et al., Proc. Natl. Acad. Sci. U.S.A. 82:5824 (1985). Ballistic transformation techniques are described in Klein et al., Nature 327:70-73 (1987).

[0139] Agrobacterium tumefaciens-meditated transformation techniques are well described in the scientific literature. See, for example Horsch et al., Science 233:496-498 (1984), and Fraley et al., Proc. Natl. Acad. Sci. 80:4803 (1983). For instance, Agrobacterium transformation of maize is described in U.S. Pat. Nos. 5,981,840; 5,591,616 and 5,731,179 herein incorporated in their entirety by reference. Agrobacterium transformation of soybean is described in U.S. Pat. No. 5,563,055.

[0140] Other methods of transformation include (1) Agrobacterium rhizogenes-mediated transformation (see, e.g., Lichtenstein and Fuller In: Genetic Engineering, Vol. 6, P. W. J. Rigby, Ed., London, Academic Press, 1987; and Lichtenstein, C. P. and Draper, J. In: DNA Cloning, Vol. II, D. M. Glover, Ed., Oxford, IRI Press, 1985), Application PCT/US87/02512 (WO 88/02405 published Apr. 7, 1988) describes the use of A. rhizogenes strain A4 and its Ri plasmid along with A. tumefaciens vectors pARC8 or pARC16, (2) liposome-mediated DNA uptake (see, e.g., Freeman et al., Plant Cell Physiol. 25:1353 (1984)), and (3) the vortexing method (see, e.g., Kindle, Proc. Natl. Acad. Sci. USA 87:1228 (1990)).

[0141] DNA can also be introduced into plants by direct DNA transfer into pollen as described by Zhou et al., Methods in Enzymology, 101:433 (1983); D. Hess, Intern Rev. Cytol., 107:367 (1987); Luo et al., Plant Mol. Biol. Reporter, 6:165 (1988). Expression of polypeptide coding polynucleotides can be obtained by injection of the DNA into reproductive organs of a plant as described by Pena et al., Nature, 325:274 (1987). DNA can also be injected directly into the cells of immature embryos and the rehydration of desiccated embryos as described by Neuhaus et al., Theor. Appl. Genet. 75:30 (1987); and Benbrook et al., in Proceedings Bio Expo 1986, Butterworth, Stoneham, Mass., pp.27-54 (1986).

[0142] Animal and lower eukaryotic (e.g., yeast) host cells are competent or rendered competent for transformation by various means. There are several well-known methods of introducing DNA into animal cells. These include: calcium phosphate precipitation, fusion of the recipient cells with bacterial protoplasts containing the DNA, treatment of the recipient cells with liposomes containing the DNA, DEAE dextran, electroporation, biolistics, and micro-injection of the DNA directly into the cells. The transfected cells are cultured by means well known in the art. Kuchler, R. J., Biochemical Methods in Cell Culture and Virology, Dowden, Hutchinson and Ross, Inc. (1977).

Transgenic Plant Regeneration

[0143] Transformed plant cells which are derived by any of the above transformation techniques can be cultured to regenerate a whole plant which possesses the transformed genotype. Such regeneration techniques often rely on manipulation of certain phytohormones in a tissue culture growth medium, typically relying on a biocide and/or herbicide marker that has been introduced together with a polynucleotide of the present invention. For transformation and regeneration of maize see, Gordon-Kamm et al., The Plant Cell 2:603-618 (1990).

[0144] Plants cells transformed with a plant expression vector can be regenerated, e.g., from single cells, callus tissue or leaf discs according to standard plant tissue culture techniques. It is well known in the art that various cells, tissues, and organs from almost any plant can be successfully cultured to regenerate an entire plant. Plant regeneration from cultured protoplasts is described in Evans et al., Protoplasts Isolation and Culture, Handbook of Plant Cell Culture, Macmillan Publishing Company, New York, pp.124-176 (1983); and Binding, Regeneration of Plants, Plant Protoplasts, CRC Press, Boca Raton, pp. 21-73 (1985).

[0145] The regeneration of plants containing the foreign gene introduced by Agrobacterium can be achieved as described by Horsch et al., Science 227:1229-1231 (1985) and Fraley et al., Proc. Natl. Acad. Sci. U.S.A. 80:4803 (1983). This procedure typically produces shoots within two to four weeks and these transformant shoots are then transferred to an appropriate root-inducing medium containing the selective agent and an antibiotic to prevent bacterial growth. Transgenic plants of the present invention may be fertile or sterile.

[0146] Regeneration can also be obtained from plant callus, explants, organs, or parts thereof. Such regeneration techniques are described generally in Klee et al., Ann. Rev. of Plant Phys. 38:467-486 (1987). The regeneration of plants from either single plant protoplasts or various explants is well known in the art. See, for example, Methods for Plant Molecular Biology, A. Weissbach and H. Weissbach, eds., Academic Press, Inc., San Diego, Calif. (1988). For maize cell culture and regeneration see generally, The Maize Handbook, Freeling and Walbot, Eds., Springer, New York (1994); Corn and Corn Improvement, 3.sup.rd edition, Sprague and Dudley Eds., American Society of Agronomy, Madison, Wis. (1988).

[0147] One of skill will recognize that after the expression cassette is stably incorporated in transgenic plants and confirmed to be operable, it can be introduced into other plants by sexual crossing. Any of a number of standard breeding techniques can be used, depending upon the species to be crossed.

[0148] In vegetatively propagated crops, mature transgenic plants can be propagated by the taking of cuttings, via production of apomictic seed, or by tissue culture techniques to produce multiple identical plants. Selection of desirable transgenics is made and new varieties are obtained and propagated vegetatively for commercial use. In seed propagated crops, mature transgenic plants can be self crossed to produce a homozygous inbred plant. The inbred plant produces seed containing the newly introduced heterologous nucleic acid. These seeds can be grown to produce plants that would produce the selected phenotype.

[0149] Parts obtained from the regenerated plant, such as flowers, seeds, leaves, branches, fruit, and the like are included in the invention, provided that these parts comprise cells comprising the isolated nucleic acid of the present invention. Progeny and variants, and mutants of the regenerated plants are also included within the scope of the invention, provided that these parts comprise the introduced nucleic acid sequences.

[0150] Transgenic plants expressing a selectable marker can be screened for transmission of the nucleic acid of the present invention by, for example, standard immunoblot and DNA detection techniques. Transgenic lines are also typically evaluated on levels of expression of the heterologous nucleic acid. Expression at the RNA level can be determined initially to identify and quantitate expression-positive plants. Standard techniques for RNA analysis can be employed and include PCR amplification assays using oligonucleotide primers designed to amplify only the heterologous RNA templates and solution hybridization assays using heterologous nucleic acid-specific probes. The RNA-positive plants can then be analyzed for protein expression by Western immunoblot analysis using the specifically reactive antibodies of the present invention. In addition, in situ hybridization and immunocytochemistry according to standard protocols can be done using heterologous nucleic acid specific polynucleotide probes and antibodies, respectively, to localize sites of expression within transgenic tissue. Generally, a number of transgenic lines are usually screened for the incorporated nucleic acid to identify and select plants with the most appropriate expression profiles.

[0151] Transgenic plants of the present invention can be homozygous for the added heterologous nucleic acid; i.e., a transgenic plant that contains two added nucleic acid sequences, one gene at the same locus on each chromosome of a chromosome pair. A homozygous transgenic plant can be obtained by sexually mating (selfing) a heterozygous transgenic plant that contains a single added heterologous nucleic acid, germinating some of the seed produced and analyzing the resulting plants produced for altered expression of a polynucleotide of the present invention relative to a control plant (i.e., native, non-transgenic). Back-crossing to a parental plant and out-crossing with a non-transgenic plant (such as Coroico) are also contemplated. Alternatively, propagation of heterozygous transgenic plants could be accomplished through apomixis.

[0152] The present invention provides a method of genotyping a plant comprising a polynucleotide of the present invention. Genotyping provides a means of distinguishing homologs of a chromosome pair and can be used to differentiate segregants in a plant population. Molecular marker methods can be used for phylogenetic studies, characterizing genetic relationships among crop varieties, identifying crosses or somatic hybrids, localizing chromosomal segments affecting monogenic traits, map based cloning, and the study of quantitative inheritance. See, e.g., Plant Molecular Biology: A Laboratory Manual, Chapter 7, Clark, Ed., Springer-Verlag, Berlin (1997). For molecular marker methods, see generally, The DNA Revolution by Andrew H. Paterson 1996 (Chapter 2) in: Genome Mapping in Plants (ed. Andrew H. Paterson) by Academic Press/R. G. Landis Company, Austin, Tex. pp. 7-21.

[0153] The particular method of genotyping in the present invention may employ any number of molecular marker analytic techniques such as, but not limited to, restriction fragment length polymorphisms (RFLPs). RFLPs are the product of allelic differences between DNA restriction fragments caused by nucleotide sequence variability. Thus, the present invention further provides a means to follow segregation of a gene or nucleic acid of the present invention as well as chromosomal sequences genetically linked to these genes or nucleic acids using such techniques as RFLP analysis.

[0154] Plants which can be used in the method of the invention include monocotyledonous and dicotyledonous plants. Typical plants include: maize, wheat, rice, barley, soybean, arabidopsis, oats, sorghum, millet, rye, sunflower, safflower, alfalfa, canola, or cotton.

[0155] Seeds derived from plants regenerated from transformed plant cells, plant parts or plant tissues, or progeny derived from the regenerated transformed plants, may be used directly as feed or food, or further processing may occur. Plants grown from these seeds can be effectively used in breeding programs to further incorporate and select for desirable traits.

[0156] All publications cited in this application are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

[0157] The present invention will be further described by reference to the following detailed examples. It is understood, however, that there are many extensions, variations, and modifications on the basic theme of the present invention beyond that shown in the examples and description, which are within the spirit and scope of the present invention.

[0158] Other objects, features, advantages and aspects of the present invention will become apparent to those of skill from the following description. It should be understood, however, that the following description and the specific examples, while indicating certain embodiments of the invention, are given by way of illustration only. Various changes and modifications within the spirit and scope of the disclosed invention will become readily apparent to those skilled in the art from reading the following description and from reading the other parts of the present disclosure.

EXAMPLES

Example 1

cDNA Library Construction

A. Total RNA Isolation

[0159] Total RNA was isolated from maize tissues with TRizol Reagent (Life Technology Inc. Gaithersburg, Md.) using a modification of the guanidine isothiocyanate/acid-phenol procedure described by Chomczynski and Sacchi (Anal. Biochem. 162, 156 (1987)). In brief, plant tissue samples were pulverized in liquid nitrogen before the addition of the TRizol Reagent, and then were further homogenized with a mortar and pestle. Addition of chloroform followed by centrifugation was conducted for separation of an aqueous phase and an organic phase. The total RNA was recovered by precipitation with isopropyl alcohol from the aqueous phase.

B. Poly(A)+ RNA Isolation

[0160] The selection of poly(A)+ RNA from total RNA was performed using PolyATract system (Promega Corporation. Madison, Wis.). In brief, biotinylated oligo(dT) primers were used to hybridize to the 3' poly(A) tails on mRNA. The hybrids were captured using streptavidin coupled to paramagnetic particles and a magnetic separation stand. The mRNA was washed at high stringent condition and eluted by RNase-free deionized water.

C. cDNA Library Construction

[0161] cDNA synthesis was performed and unidirectional cDNA libraries were constructed using the SuperScript Plasmid System (Life Technology Inc. Gaithersburg, Md.). The first stand of cDNA was synthesized by priming an oligo(dT) primer containing a Not I site. The reaction was catalyzed by SuperScript Reverse Transcriptase II at 45.degree. C. The second strand of cDNA was labeled with alpha-.sup.32P-dCTP and a portion of the reaction was analyzed by agarose gel electrophoresis to determine cDNA sizes. cDNA molecules smaller than 500 base pairs and unligated adapters were removed by Sephacryl-S400 chromatography. The selected cDNA molecules were ligated into pSPORT1 vector in between Not I and Sal I sites.

D. dek1 Isolation and Sequencing

[0162] dek1 maize cDNA fragments were used as probes to screen a maize Mo17 genomic bacterial artificial chromosome (BAC) library. The HindIII and EcoRI subfragments homologous to calcium-requiring cysteine proteinases (calpain) cDNA were identified in selected BAC clones by gel blot hybridization, cloned into a plasmid vector pBKSKS (Stratagene), and sequenced with the help of the ES::TN transposon insertion system (Epicentre Technologies, Madison, Wis.).

Example 2

Sequencing and cDNA Subtraction Procedures used for Maize EST's

A. Sequencing Template Preparation

[0163] Individual colonies were picked and DNA was prepared either by PCR with M13 forward primers and M13 reverse primers, or by plasmid isolation. All the cDNA clones were sequenced using M13 reverse primers.

B. Q-Bot Subtraction Procedure

[0164] cDNA libraries subjected to the subtraction procedure were plated out on 22.times.22 cm.sup.2 agar plate at density of about 3,000 colonies per plate. The plates were incubated in a 37.degree. C. incubator for 12-24 hours. Colonies were picked into 384-well plates by a robot colony picker, Q-bot (GENETIX Limited). These plates were incubated overnight at 37.degree. C.

[0165] Once sufficient colonies were picked, they were pinned onto 22.times.22 cm.sup.2 nylon membranes using Q-bot. Each membrane contained 9,216 colonies or 36,864 colonies. These membranes were placed onto individual agar plates with appropriate antibiotic. The plates were incubated at 37.degree. C. for overnight.

[0166] After colonies were recovered on the second day, these filters were placed on filter paper prewetted with denaturing solution for four minutes, then were incubated on top of a boiling water bath for additional four minutes. The filters were then placed on filter paper prewetted with neutralizing solution for four minutes. After excess solution was removed by placing the filters on dry filter papers for one minute, the colony side of the filters were place into Proteinase K solution, incubated at 37.degree. C. for 40-50 minutes. The filters were placed on dry filter papers to dry overnight. DNA was then cross-linked to nylon membrane by UV light treatment.

[0167] Colony hybridization was conducted as described by Sambrook, J., Fritsch, E. F. and Maniatis, T., (in Molecular Cloning: A Laboratory Manual, 2.sup.nd Edition). The following probes were used in colony hybridization: [0168] 1. First strand cDNA from the same tissue from which the library was made to remove the most redundant clones. [0169] 2. 48-192 most redundant cDNA clones from the same library based on previous sequencing data. [0170] 3. 192 most redundant cDNA clones in the entire corn sequence database. [0171] 4. A Sal-A20 oligonucleotide removes clones containing a poly A tail but no cDNA. See SEQ ID NO:22. [0172] 5. cDNA clones derived from rRNA.

[0173] The image of the autoradiography was scanned into computer and the signal intensity and cold colony addresses of each colony was analyzed. Re-arraying of cold-colonies from 384 well plates to 96 well plates was conducted using Q-bot.

Example 3

Co-Segregation Analysis of Superal Mutation

[0174] The maize superal mutation is characterized by multiple aleurone layers. Unlike wild-type maize, superal maize can have up to 8 layers of aleurone. It was isolated from the Pioneer TUSC population by phenotypic analysis; aleurone layers showed a red color when stained with the oil-specific stain red-fat7B. In the TUSC collection, a separate mutant with multiple aleurone layers was isolated by a microscopy screen, which when crossed to superal, demonstrated that this represents a second and independent allele.

[0175] The superal mutation is recessive and showed a simple 3:1 segregation in selfed ears.

[0176] Southern blots of the backcross population showed that a 1.6 kb BgIII fragment of mu1 (Del Giudice, L., et al, Mol. Gen. Genet. 222 (1), 71-76 (1990)) was co-segregated with the superal phenotype. When the band was cloned, the sequences flanking the mu1 insertion showed identity to SEQ ID NO:5. SEQ ID NO:5 showed complete co-segregation with the superal phenotype, consistent with this gene as the causal agent for the superal phenotype.

[0177] Mu1 was inserted in the 5'-UTR of the superal gene. A second independent allele with a mu8 (Fleenor, D., et al, Nucleic Acids Res. 18 (22), 6725 (1990)) insertion at 3'-UTR of superal gene showed the similar multiple aleurone layers, demonstrating that mutations resulting in down-regulation of SUPERAL was responsible for the multi-aleurone layer phenotype.

Example 4

Co-Segregation Analysis of dek1 Mutation

[0178] The maize Dek1 mutation is characterized by a lack of aleurone layers.

[0179] Southern blots of a segregating population showed that plants with the Dek1 mutant phenotype did not contain a 3.0 kb KpnI fragment representing the wild-type band. The plants were probed with the sequence flanking the co-segregating Mu1 insertion, indicating that maize Dek1 is responsible for the dek1 phenotype.

[0180] A second dek1 allele is being characterized.

Example 5

To Assay Gene Functionality

Dek1

[0181] Grain of the genotype dek1/dek1 (lacking aleurone cells) from Dek1/dek1 plants cultured in vitro had pericarp removed and were bombarded with constructs comprising maize Dek1 behind the beps promoter (Thorbjornsen, T. et al., 1996, Biochem J., 313:149-154, pp 152) driving gene transcription in peripheral starchy endosperm cells, and were co-bombarded with constructs comprising anthocyanin activators c1 (Paz-Ares et al., Embo J. 6(12):3553-8, 1987) and Ic (Ludwig, S. R. et al., Natl Acad Sci US 86(18):7092-6,1989) behind the 35S promoter. Control constructs comprised only the activators with the 35S promoter.

[0182] Positive results were represented by kernels with red anthocyanine spots due to the presence of aleurone cells. Controls give no red spots due to lack of aleurone cells, as red anthocyanin coloration is expressed in aleurone cells but not starchy endosperm cells.

[0183] Alternatively, cyncytial stage dek1/dek1 endosperm of in vitro nucellus slab culture is micro injected with Dek1. Sectors of endosperm with aleurone cells, stemming from single nuclei or groups of nuclei in which Dek1 was incorporated, proves the function of Dek1 in aleurone cell signaling. The presence of aleurone cells was facilitated by using dek1/Dek1 plants that contained Ltp2::Gus gene constructs. In this genetic background, aleurone cells were detectable by the presence of the blue Gus stain. In these endosperms, the dek1/dek1 genotype of the endosperm was shown by the dek1/dek1 phenotype of the embryo, which lacks axis polarity. In these nucellus slab cultures, the embryos were never or extremely rarely transformed by injected DNA.

Superal

[0184] The function of the Superal gene as a negative regulator of aleurone cell fate in endosperm cells other than the peripheral layer of the endosperm was shown in co-bombardment experiments of in vitro cultivated colorless wild type grains with an Ltp2::superal cDNA construct and constructs comprising anthocyanin activators c1 (Paz-Ares, et al., Embo J. 6(12):3553-8,1987) and Ic (Ludwig, S. R. et al., Natl Acad Sci US 86(18):7092-6, 1989) behind the 35S promoter. Control constructs comprised only the activators with the 35S promoter. Positive results were represented by kernels in which red anthocyanine spots did not appear or disappeared shortly after bombardment due to the de-differentiation of aleurone cells to starchy endosperm cells caused by ectopic expression of Superal in aleurone cells.

[0185] Alternatively, bombardment of in vitro cultivated grains with a genotype that gives red aleurone cells with the beps promoter::superal cDNA, demonstrate the wild-type function of SUPERAL as a suppressor of aleurone cell fate by the presence of white spots due to the de-differentiation of aleurone cells to starchy endosperm cells. Control bombardment with other genes under the control of the 35S promoter had no effect on aleurone cell coloration.

[0186] Alternatively, the function of Superal was demonstrated by the injection of Superal in homozygous superal syncytial endosperm of nucellus slab cultures. Endosperm with normal aleurone layers or sectors with normal aleurone layers demonstrated the wild-type function of Superal as a negative regulator of aleurone cell fate in deeper layers of the endosperm. Identification of the injected seeds as homozygous superal seeds was done by the embryo phenotype: homozygous superal embryos being arrested at an early developmental stage. The embryo was never or very rarely transformed by the injected DNA in nucellus slab cultures.

[0187] Alternatively, the function of SUPERAL is demonstrated by co-suppressing superal expression in transgenic seed. The superal gene introduced into plants under a seed specific promoter to silence endogenous superal gene expression, results in multi-aleurone layers.

Example 6

Vector Construction

[0188] All vectors are constructed using standard molecular biology techniques used by those of skill in the art (Sambrook et al., supra). Vectors are constructed for plant transformation using both particle bombardment and Agrobacterium transformation protocols. Plasmids are constructed by inserting the gene of interest into an expression cassette. For example, the Dek1 coding region, including the 5' UTR and 3' UTR is isolated from a full length EST clone. The fragment is ligated into an expression cassette comprising the beps promoter and nos terminator. Orientation is confirmed using a restriction enzyme digest. The expression cassette is linked to the selectable marker between the right and left borders of the T-DNA borders of an appropriate vector for Agrobacterium transformation. This vector is used for insert preparation for particle gun transformation as well as for generating Agrobacterium transformation vectors as described below. In this case, insert DNA for particle gun transformation is generated by isolating the insert from the vector.

[0189] The plasmid pSB11 is obtained from Japan Tobacco Inc. (Tokyo, Japan). The construction of pSB11 from pSB21 and the construction of pSB21 from starting vectors is described by Komari et al., 1996, Plant J. 10:165-174. The T-DNA of the plasmid is integrated in to the superbinary plasmid pSB1 (Saito et al., EP 672 752 A1) by homologous recombination between the two plasmids. The plasmid pSB1 is also obtained from Japan Tobacco Inc. These plasmids are either used for particle bombardment transformation, or for Agrobacterium-mediated transformation, after making a cointegrate in an appropriate Agrobacterium strain.

[0190] Competent cells of the Agrobacterium strain LBA4404 harboring pSB1 are created using the protocol as described by Lin (1995) in Methods in Molecular Biology, ed. Nickoloff, J. A. (Humana Press, Totowa, N.J.). The plasmid containing the expression cassettes is electroporated into competent cells of the Agrobacterium strain LBA4404 harboring pSB1 to create the cointegrate plasmid in Agrobacterium. Cells and DNA are prepared for electroporation by mixing 1 ul of plasmid DNA (.about.100ng) with 20 ul of competent Agrobacterium cells in a 0.2 cm electrode gap cuvette (Bio-Rad Cat# 165-2086, Hercules, Calif.). Electroporation is performed in a Bio-Rad Micropulser (Cat#165-2100, Hercules, Calif.) using the EC2 setting, which delivers 2.5 kV to the cells. Successful recombination is verified by restriction analysis of the plasmid after transformation of the cointegrate plasmid back into E. coli DH5.alpha. cells.

Example 7

Plant Transformation

A. Particle Bombardment Transformation and Regeneration of Maize Callus

[0191] Immature maize embryos from greenhouse or field grown High type II donor plants are bombarded with a plasmid containing a calpain polynucleotide of the invention operably linked to an appropriate promoter. If the polynucleotide does not include a selectable marker, another plasmid containing a selectable marker gene can be co-precipitated on the particles used for bombardment. For example, a plasmid containing the PAT gene (Wohlleben et al., 1988, Gene 70:25-37) which confers resistance to the herbicide Bialaphos can be used. Transformation is performed as follows.

[0192] The ears are surface sterilized in 50% Chlorox bleach plus 0.5% Micro detergent for 20 minutes, and rinsed two times with sterile water. The immature embryos are excised and placed embryo axis side down (scutellum side up), 25 embryos per plate. These are cultured on 560L agar medium 4 days prior to bombardment in the dark. Medium 560L is an N6-based medium containing Eriksson's vitamins, thiamine, sucrose, 2,4-D, and silver nitrate. The day of bombardment, the embryos are transferred to 560Y medium for 4 hours and are arranged within the 2.5-cm target zone. Medium 560Y is a high osmoticum medium (560L with high sucrose concentration).

[0193] A plasmid vector comprising a polynucleotide of the invention operably linked to the selected promoter is constructed. This plasmid DNA, plus plasmid DNA containing a PAT selectable marker if needed, is precipitated onto 1.1 .mu.m (average diameter) tungsten pellets using a CaCl.sub.2 precipitation procedure as follows: 100 .mu.l prepared tungsten particles (0.6 mg) in water, 20 .mu.l (2 .mu.g) DNA in TrisEDTA buffer (1 .mu.g total), 100 .mu.l 2.5 M CaCl.sub.2, 40 .mu.l 0.1 M spermidine.

[0194] Each reagent is added sequentially to the tungsten particle suspension. The final mixture is sonicated briefly. After the precipitation period, the tubes are centrifuged briefly, liquid removed, washed with 500 ml 100% ethanol, and centrifuged again for 30 seconds. Again the liquid is removed, and 60 .mu.l 100% ethanol is added to the final tungsten particle pellet. For particle gun bombardment, the tungsten/DNA particles are briefly sonicated and 5 .mu.l spotted onto the center of each macrocarrier and allowed to dry about 2 minutes before bombardment.

[0195] The sample plates are bombarded at a distance of 8 cm from the stopping screen to the tissue, using a DuPont biolistics helium particle gun. All samples receive a single shot at 650 PSI, with a total of ten aliquots taken from each tube of prepared particles/DNA.

[0196] Four to 12 hours post bombardment, the embryos are moved to 560P (a low osmoticum callus initiation medium similar to 560L but with lower silver nitrate), for 3-7 days, then transferred to 560R selection medium, an N6 based medium similar to 560P containing 3 mg/liter Bialaphos, and subcultured every 2 weeks. After approximately 10 weeks of selection, callus clones are sampled for PCR and activity of the polynucleotide of interest. Positive lines are transferred to 288J medium, an MS-based medium with lower sucrose and hormone levels, to initiate plant regeneration. Following somatic embryo maturation (2-4 weeks), well-developed somatic embryos are transferred to medium for germination and transferred to the lighted culture room. Approximately 7-10 days later, developing plantlets are transferred to medium in tubes for 7-10 days until plantlets are well established. Plants are then transferred to inserts in flats (equivalent to 2.5'' pot) containing potting soil and grown for 1 week in a growth chamber, subsequently grown an additional 1-2 weeks in the greenhouse, then transferred to Classic.TM. 600 pots (1.6 gallon) and grown to maturity. Plants are monitored for expression of the polynucleotide of interest.

B. Agrobacterium-Mediated Transformation and Regeneration of Maize Callus

[0197] For Agrobacterium-mediated transformation of maize of a nucleotide sequence encoding a protein of the present invention, the method of Zhao was employed (U.S. Pat. No. 5,981,840, and PCT patent publication WO98/32326; the contents of which are hereby incorporated by reference).

[0198] Briefly, immature embryos were isolated from maize and the embryos contacted with a suspension of Agrobacterium containing a polynucleotide of the present invention, where the bacteria are capable of transferring the nucleotide sequence of interest to at least one cell of at least one of the immature embryos (step 1: the infection step). In this step the immature embryos were immersed in an Agrobacterium suspension for the initiation of inoculation. The embryos were co-cultured for a time with the Agrobacterium (step 2: the co-cultivation step). The immature embryos were cultured on solid medium following the infection step. Following this co-cultivation period an optional "resting" step is contemplated. In this resting step, the embryos were incubated in the presence of at least one antibiotic known to inhibit the growth of Agrobacterium without the addition of a selective agent for plant transformants (step 3: resting step). The immature embryos were cultured on solid medium with antibiotic, but without a selecting agent, for elimination of Agrobacterium and for a resting phase for the infected cells. Next, inoculated embryos were cultured on medium containing a selective agent and growing transformed callus was recovered (step 4: the selection step). The immature embryos were cultured on solid medium with a selective agent resulting in the selective growth of transformed cells. The callus was then regenerated into plants (step 5: the regeneration step), and calli grown on selective medium were cultured on solid medium to regenerate the plants.

C. DNA Isolation from Callus and Leaf Tissues

[0199] In order to screen putative transformation events for the presence of the transgene, genomic DNA is extracted from calluses or leaves using a modification of the CTAB (cetyltriethylammonium bromide, Sigma H5882) method described by Stacey and Isaac (1994). Approximately 100-200 mg of frozen tissues is ground into powder in liquid nitrogen and homogenised in 1 ml of CTAB extraction buffer (2% CTAB, 0.02 M EDTA, 0.1 M Tris-Cl pH 8, 1.4 M NaCl, 25 mM DTT) for 30 min at 65.degree. C. Homogenised samples are allowed to cool at room temperature for 15 min before a single protein extraction with approximately 1 ml 24:1 v/v chloroform:octanol is done. Samples are centrifuged for 7 min at 13,000 rpm and the upper layer of supernatant collected using wide-mouthed pipette tips. DNA is precipitated from the supernatant by incubation in 95% ethanol on ice for 1 h. DNA threads are spooled onto a glass hook, washed in 75% ethanol containing 0.2 M sodium acetate for 10 min, air-dried for 5 min and resuspended in TE buffer. Five .mu.l RNAse A is added to the samples and incubated at 37.degree. C. for 1 h.

[0200] For quantification of genomic DNA, gel electrophoresis is performed using a 0.8% agarose gel in 1.times. TBE buffer. One microlitre of the samples are fractionated alongside 200, 400, 600 and 800 ng .mu.l.sup.-1 .lamda. uncut DNA markers.

Example 8

Overexpression of CR4 in Transgenic Corn

[0201] The maize cr4 gene (SEQ ID NO:15) was overexpressed in endosperm under the control of the Itp2 promoter (Kalla, R., et al., 1994) (Itp2::cr4::pinII) and 22 kDa zein promoter (Boronat, A., et al., 1986) (22 kDa zein::cr4::22 kDa zein) constructed as generally described in Example 6. A single vector containing both constructs was introduced into maize by agrobacterial transformation (see Example 7B). Transgenic plants showed an extra layer of aleurone cells in the endosperm. This additional layer of cells stained red by red fat-7B, indicating oil accumulation in the extra aleurone cells.

Example 9

Expression of Functional Domains in Bacteria and Plants

[0202] Functional domains that were used in bacterial and plant expression constructs were as follows: [0203] External loop region: positions 1063-1938 of SEQ ID NO:23 corresponding to positions 337-628 of SEQ ID NO:24 [0204] Cytoplasmic domain: positions 3679-6531 of SEQ ID NO:23 corresponding to positions 1209-2159 of SEQ ID NO:24 [0205] calpain proteinase Domain II: positions 5164-6069 of SEQ ID NO:23 corresponding to positions 1704-2005 of SEQ ID NO:24 [0206] calpain proteinase Domain III: positions 6082-6531 of SEQ ID NO:23 corresponding to positions 2010-2159 of SEQ ID NO:24 A. Bacterial Expression

[0207] Calpain proteinase Domains II+III, and the external loop region were cloned into the pGEX-4T-3 vector (Amersham Biosciences) for expression in E. coli. After induction with IPTG, the bacterial lysates were analyzed for the presence of expressed protein. Proteins are expressed as fusion proteins with the 26 kDa glutathione S-transferase. Both constucts were shown to have GST activity.

[0208] Sequence analysis has indicated that dek1 has domain features similar to calpain. Domain II & III of calpain are highly conserved in dek1. In addition, it is reported that domain II of m-calpain has cysteine protease activity. Therefore, bacteria-expressed domain II+III of dek1 was used to determine if dek1 has calpain activity.

[0209] The so called caseinolytic assay was used for this determination. In this assay, degradation of casein was evaluated by electrophoresis on SDS-PAGE with gel staining. With partially purified Domain II+III, casein was clearly shown to be degraded. Caseinolytic activity of partially purified Domain II+III was higher than that of domain II of human m-calpain as reported.

B. Plant Expression

[0210] The external loop region, cytoplasmic domain, calpain proteinase domain II, and calpain proteinase domain III are each operably linked to the Itp2 and/or 22 kDa zein promoter to express altered aleurone phenotypes in plants.

[0211] Over-expression of the external loop region is expected to phenocopy the dek1 mutation, ie: lack of aleurone cells.

[0212] Expression of the cytoplasmic domain, domain II, and domain III are expected to increase the number of aleurone layers. TABLE-US-00001 Sequence Listing Table Nucleotide (NT) or SEQ ID NO. Description Amino Acid (AA) 1 maize dek1 cDNA incomplete sequence NT 2 maize dek1 incomplete sequence AA 3 Arabidopsis calpain cDNA NT 4 Arabidopsis calpain AA 5 maize superal cDNA NT 6 maize SUPERAL AA 7 Arabidopsis superal cDNA homolog 1 NT 8 Arabidopsis SUPERAL homolog 1 AA 9 Arabidopsis superal cDNA homolog 2 NT 10 Arabidopsis SUPERAL homolog 2 AA 11 Rice superal homolog NT 12 Wheat superal homolog NT 13 Soybean superal homolog NT 14 Barley superal homolog NT 15 maize crinkly4 (cr4) cDNA NT 16 maize CRINKLY 4 AA 17 barley nuc1 promoter NT 18 barley Ltp2 promoter NT 19 maize end1 promoter NT 20 maize end2 promoter NT 21 barley beps promoter NT 22 Sal A20 primer NT 23 maize dek1 cDNA from B73 NT 24 maize DEK1 from B73 AA 25 maize Mo17 dek1 (genomic) NT 26 Arabidopsis dek1 cDNA NT 27 Arabidopsis DEK1 AA 28 Arabidopsis dek1 (genomic) NT

[0213]

Sequence CWU 1

1

28 1 4857 DNA Zea mays CDS (1)...(4857) maize dek1 1 ctc tca ttt gct gtt ccc ata tgg ata cgc aat ggt tac agt ttc tgg 48 Leu Ser Phe Ala Val Pro Ile Trp Ile Arg Asn Gly Tyr Ser Phe Trp 1 5 10 15 att cct gga agg gag ttt gca aat cgt gaa aat gtt agt caa gct cca 96 Ile Pro Gly Arg Glu Phe Ala Asn Arg Glu Asn Val Ser Gln Ala Pro 20 25 30 gga gag aaa gag cgg gct ctc ttt gtt atc acc att gct gtt ttc act 144 Gly Glu Lys Glu Arg Ala Leu Phe Val Ile Thr Ile Ala Val Phe Thr 35 40 45 gca tca att att ggc ctt ggt gca ata gtg tca gca aag cct tta gac 192 Ala Ser Ile Ile Gly Leu Gly Ala Ile Val Ser Ala Lys Pro Leu Asp 50 55 60 gct cta ggc tat aaa gga tgg gat gct gat aag aac agc tcc tat tct 240 Ala Leu Gly Tyr Lys Gly Trp Asp Ala Asp Lys Asn Ser Ser Tyr Ser 65 70 75 80 ccc tat gca aca tca atg tat ctt gga tgg gca ttg tct tca aca att 288 Pro Tyr Ala Thr Ser Met Tyr Leu Gly Trp Ala Leu Ser Ser Thr Ile 85 90 95 gct gtg att acc aca ggg ttg ata cct att gtt gct tgg ttt gca aca 336 Ala Val Ile Thr Thr Gly Leu Ile Pro Ile Val Ala Trp Phe Ala Thr 100 105 110 tac cgg ttt tca cct tca tca gct ata tgt gtt ggc ctc ttt gca act 384 Tyr Arg Phe Ser Pro Ser Ser Ala Ile Cys Val Gly Leu Phe Ala Thr 115 120 125 gtt ctt gtg tct ttt tgc ggt gca tcc tac tgg gga gtg gta aat tca 432 Val Leu Val Ser Phe Cys Gly Ala Ser Tyr Trp Gly Val Val Asn Ser 130 135 140 cga gag gat ggt gtt cct cta aag gct gat ttc ctt gca gca tta ctt 480 Arg Glu Asp Gly Val Pro Leu Lys Ala Asp Phe Leu Ala Ala Leu Leu 145 150 155 160 ccc ttg ctt tgc att cca gca ttt ttc tca ctg ttc act ggg ctt tac 528 Pro Leu Leu Cys Ile Pro Ala Phe Phe Ser Leu Phe Thr Gly Leu Tyr 165 170 175 aaa tgg aag gat gat gat tgg aag att tct cgt ggt gtt tac ctt ttt 576 Lys Trp Lys Asp Asp Asp Trp Lys Ile Ser Arg Gly Val Tyr Leu Phe 180 185 190 gtt ggc atg gga atg ttg ctg ttg ttt ggt gca gtt gca gct gtt att 624 Val Gly Met Gly Met Leu Leu Leu Phe Gly Ala Val Ala Ala Val Ile 195 200 205 gtc aca atc agg ccc tgg act gtt gga gtt gct tgc ctc gta gcc att 672 Val Thr Ile Arg Pro Trp Thr Val Gly Val Ala Cys Leu Val Ala Ile 210 215 220 ctg ttc ctt gta ttt gtt att ggg gtc atc cac tac tgg aca tct aac 720 Leu Phe Leu Val Phe Val Ile Gly Val Ile His Tyr Trp Thr Ser Asn 225 230 235 240 aac ttc tat cta acg agg act cag atg ttg ctt gtt tgt tcc att gct 768 Asn Phe Tyr Leu Thr Arg Thr Gln Met Leu Leu Val Cys Ser Ile Ala 245 250 255 ttt ctc tta gcc ttg gct gcc ttc ctg atg ggt tta ttt cac gga aag 816 Phe Leu Leu Ala Leu Ala Ala Phe Leu Met Gly Leu Phe His Gly Lys 260 265 270 cct ttt gtt gga gca tct ata ggt tat ttc tca ttt ata ttt ctt ctc 864 Pro Phe Val Gly Ala Ser Ile Gly Tyr Phe Ser Phe Ile Phe Leu Leu 275 280 285 act gga agg gct ttg act gtc ctt cta tca ccg cca atc gta gtg tat 912 Thr Gly Arg Ala Leu Thr Val Leu Leu Ser Pro Pro Ile Val Val Tyr 290 295 300 tcg cca aga gta ttg cct gta tac gtt tat gat gct cat gca gac tct 960 Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His Ala Asp Ser 305 310 315 320 gct aaa aat gtt agc tat gcc ttt ctt att ctg tat ggg att gca tta 1008 Ala Lys Asn Val Ser Tyr Ala Phe Leu Ile Leu Tyr Gly Ile Ala Leu 325 330 335 gca act gaa gtt tgg ggt gtt att gct agt cta ata atg aat cca cca 1056 Ala Thr Glu Val Trp Gly Val Ile Ala Ser Leu Ile Met Asn Pro Pro 340 345 350 ttt gtt ggg gct ggc gtt tct gct act act ctt gta att gct ttc agt 1104 Phe Val Gly Ala Gly Val Ser Ala Thr Thr Leu Val Ile Ala Phe Ser 355 360 365 ttt gct gtt tct cga cca tgc ctg act ctt aag atg atg gag gat gca 1152 Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Lys Met Met Glu Asp Ala 370 375 380 gtt cat ttt ctc agc aag gat aca gtt gtg caa gcg atg tca cgg tct 1200 Val His Phe Leu Ser Lys Asp Thr Val Val Gln Ala Met Ser Arg Ser 385 390 395 400 gct aat aaa act aga aat gct ata tct ggg act tac tca gca cct cag 1248 Ala Asn Lys Thr Arg Asn Ala Ile Ser Gly Thr Tyr Ser Ala Pro Gln 405 410 415 agg tcc gca agt tct gct gct ctt ttg gtt gga gat cct gct ctt aca 1296 Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro Ala Leu Thr 420 425 430 ttg gac agg gct ggg aac ttt gtg ctt cct agg gct gat gtt atg aaa 1344 Leu Asp Arg Ala Gly Asn Phe Val Leu Pro Arg Ala Asp Val Met Lys 435 440 445 ctg aga gat cgt ttg aga aat gaa gaa att gct gca gga tct ttc tta 1392 Leu Arg Asp Arg Leu Arg Asn Glu Glu Ile Ala Ala Gly Ser Phe Leu 450 455 460 tgt gga gta aaa gat tgt tta cta att tgc ccc cag tcc ctg tca aac 1440 Cys Gly Val Lys Asp Cys Leu Leu Ile Cys Pro Gln Ser Leu Ser Asn 465 470 475 480 ata gat tat cgg agg aat atg tgt gcc cat gca cgt att ttg gct ttg 1488 Ile Asp Tyr Arg Arg Asn Met Cys Ala His Ala Arg Ile Leu Ala Leu 485 490 495 gaa gaa gca att gat aca gaa tgg gtg tat atg tgg gac aaa ttt ggt 1536 Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp Lys Phe Gly 500 505 510 ggt tat tta ctt ctg ttg ctt gga ttg act gcc aaa gct gaa caa ata 1584 Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala Glu Gln Ile 515 520 525 cag gat gaa gtt cgt cta aga ctc ttt ttg gat agc ata ggc ctt tcc 1632 Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile Gly Leu Ser 530 535 540 gat ttg agt gcc aaa gaa att aag aaa tgg atg cct gaa gat cgg agg 1680 Asp Leu Ser Ala Lys Glu Ile Lys Lys Trp Met Pro Glu Asp Arg Arg 545 550 555 560 caa ttt gag ctt att caa gaa agc tac ata agg gaa aaa gaa atg gaa 1728 Gln Phe Glu Leu Ile Gln Glu Ser Tyr Ile Arg Glu Lys Glu Met Glu 565 570 575 gag gag gct ttg atg caa aga cga gag gaa gaa ggg aag gga aga gaa 1776 Glu Glu Ala Leu Met Gln Arg Arg Glu Glu Glu Gly Lys Gly Arg Glu 580 585 590 agg agg agg gca ttg cta gag aga gag gag cga aaa tgg aag gag ctc 1824 Arg Arg Arg Ala Leu Leu Glu Arg Glu Glu Arg Lys Trp Lys Glu Leu 595 600 605 gaa ata tca ttg ctt tct tcc att cca aat act gga agc agg gat gct 1872 Glu Ile Ser Leu Leu Ser Ser Ile Pro Asn Thr Gly Ser Arg Asp Ala 610 615 620 gca gct atg gca gca gct gtc aga gct gtt gga ggt gat tct gcc ctg 1920 Ala Ala Met Ala Ala Ala Val Arg Ala Val Gly Gly Asp Ser Ala Leu 625 630 635 640 gaa gat tct ttt gca aga gat agg gtc tct tca ata gcc aat cac ata 1968 Glu Asp Ser Phe Ala Arg Asp Arg Val Ser Ser Ile Ala Asn His Ile 645 650 655 cga aag gca caa ttg gct cgg cga gca gaa cag act ggt att cca ggc 2016 Arg Lys Ala Gln Leu Ala Arg Arg Ala Glu Gln Thr Gly Ile Pro Gly 660 665 670 act ata tgc ata ctc gat gat gaa cca agg agt act ggg cgt cat tgt 2064 Thr Ile Cys Ile Leu Asp Asp Glu Pro Arg Ser Thr Gly Arg His Cys 675 680 685 gga gag ctt gac ttg tgc ctc tgt caa agt caa aag gtt act ttg tct 2112 Gly Glu Leu Asp Leu Cys Leu Cys Gln Ser Gln Lys Val Thr Leu Ser 690 695 700 att gct gtc atg gtt cag cct gta tct ggc cca gtg tgt ctt ttt gga 2160 Ile Ala Val Met Val Gln Pro Val Ser Gly Pro Val Cys Leu Phe Gly 705 710 715 720 agt gaa ttc caa aag gtt tgt tgg gaa atc tta gtg gca gga tca gaa 2208 Ser Glu Phe Gln Lys Val Cys Trp Glu Ile Leu Val Ala Gly Ser Glu 725 730 735 cag ggt atg gaa gct gga caa gtt ggt ctt cga tta gta act aag ggt 2256 Gln Gly Met Glu Ala Gly Gln Val Gly Leu Arg Leu Val Thr Lys Gly 740 745 750 gaa agg atg act act gtt gct aaa gag tgg aat att ggt gcg tct agt 2304 Glu Arg Met Thr Thr Val Ala Lys Glu Trp Asn Ile Gly Ala Ser Ser 755 760 765 att gca gat ggc agg tgg cat ctt gtc act gta act tta gat gcc gac 2352 Ile Ala Asp Gly Arg Trp His Leu Val Thr Val Thr Leu Asp Ala Asp 770 775 780 cta ggt gaa gca act tct ttc att gat gga gtt tat gat gga tat cag 2400 Leu Gly Glu Ala Thr Ser Phe Ile Asp Gly Val Tyr Asp Gly Tyr Gln 785 790 795 800 aat ggg ttg ccg ttg cca aca gat aac ggt att tgg gaa cct gga act 2448 Asn Gly Leu Pro Leu Pro Thr Asp Asn Gly Ile Trp Glu Pro Gly Thr 805 810 815 gat att tgg gtt ggt gct agg cca ccc atg gac tta gat gcc ttt ggt 2496 Asp Ile Trp Val Gly Ala Arg Pro Pro Met Asp Leu Asp Ala Phe Gly 820 825 830 agg tca gat agc gaa ggt tct gac tca aag atg cag atc atg gat gct 2544 Arg Ser Asp Ser Glu Gly Ser Asp Ser Lys Met Gln Ile Met Asp Ala 835 840 845 ttt cta tgg gga aga tgt ctc agt gaa gat gag gtt act gtt tta cat 2592 Phe Leu Trp Gly Arg Cys Leu Ser Glu Asp Glu Val Thr Val Leu His 850 855 860 act gcc atg tct cct gct gag tat gga ttt ttt gac ctt gca ccc ggc 2640 Thr Ala Met Ser Pro Ala Glu Tyr Gly Phe Phe Asp Leu Ala Pro Gly 865 870 875 880 gat gct tgg cat gga agt tat tct gca agg gtg gat gac tgg gaa agc 2688 Asp Ala Trp His Gly Ser Tyr Ser Ala Arg Val Asp Asp Trp Glu Ser 885 890 895 gaa gag gct tat gag ctt tat gat caa ggg gat gtc gaa tgg gat gga 2736 Glu Glu Ala Tyr Glu Leu Tyr Asp Gln Gly Asp Val Glu Trp Asp Gly 900 905 910 cag tac tca agt ggt agg aaa cgt ccg gta cat gat gct gta gct att 2784 Gln Tyr Ser Ser Gly Arg Lys Arg Pro Val His Asp Ala Val Ala Ile 915 920 925 gac ctt gac tcc ttt gct agg aga cca aga aaa cca agg ttt gag aca 2832 Asp Leu Asp Ser Phe Ala Arg Arg Pro Arg Lys Pro Arg Phe Glu Thr 930 935 940 cgt gat gaa gtc aac cag cgt atg ctt tct gtt gaa agg gct gtc agg 2880 Arg Asp Glu Val Asn Gln Arg Met Leu Ser Val Glu Arg Ala Val Arg 945 950 955 960 gat gct ctt atc gcg aaa gga gag aga aac ttc act gat caa gag ttc 2928 Asp Ala Leu Ile Ala Lys Gly Glu Arg Asn Phe Thr Asp Gln Glu Phe 965 970 975 cct cca gag gat cgt tct tta ttt gta gat ccg atg aat cca cct ctg 2976 Pro Pro Glu Asp Arg Ser Leu Phe Val Asp Pro Met Asn Pro Pro Leu 980 985 990 aaa ctg cag gtt gtt tct gag tgg atg agg cct tct gac ata gca aag 3024 Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro Ser Asp Ile Ala Lys 995 1000 1005 gat ata tct atc agt tgt cag cct tgc ttg ttt tcg ggt tct gtg aat 3072 Asp Ile Ser Ile Ser Cys Gln Pro Cys Leu Phe Ser Gly Ser Val Asn 1010 1015 1020 tcc tca gat gtg tgt cag ggt cgg ttg gga gac tgt tgg ttc cta agt 3120 Ser Ser Asp Val Cys Gln Gly Arg Leu Gly Asp Cys Trp Phe Leu Ser 1025 1030 1035 1040 gca gtc gca gtt tta act gag atg tct cgg ata tca gaa gtt ata atc 3168 Ala Val Ala Val Leu Thr Glu Met Ser Arg Ile Ser Glu Val Ile Ile 1045 1050 1055 act ccc gag tac aat gat gaa ggg att tat aca gtc aga ttc tgt att 3216 Thr Pro Glu Tyr Asn Asp Glu Gly Ile Tyr Thr Val Arg Phe Cys Ile 1060 1065 1070 cag ggt gag tgg gtg gcc gtg gtt gtt gat gat tgg att cct tgc gag 3264 Gln Gly Glu Trp Val Ala Val Val Val Asp Asp Trp Ile Pro Cys Glu 1075 1080 1085 tct ccg ggg aaa cca gca ttt gct act agt aga aag caa aac gag ctt 3312 Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg Lys Gln Asn Glu Leu 1090 1095 1100 tgg gta tcc att ctt gag aag gct tat gca aaa ctt cat ggc tct tat 3360 Trp Val Ser Ile Leu Glu Lys Ala Tyr Ala Lys Leu His Gly Ser Tyr 1105 1110 1115 1120 gag gca ttg gaa ggt ggg ctt gtt caa gat gct cta gtc gat ctc aca 3408 Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala Leu Val Asp Leu Thr 1125 1130 1135 gga gga gct ggt gaa gag att gat atg cga agt cct caa gcc caa ctt 3456 Gly Gly Ala Gly Glu Glu Ile Asp Met Arg Ser Pro Gln Ala Gln Leu 1140 1145 1150 gat ctt gct agt gga aga ttg tgg tcg cag ttg ttg cat ttc aaa caa 3504 Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu Leu His Phe Lys Gln 1155 1160 1165 gaa ggt ttt ctt ctt ggt gct gga agt cct tct gga tct gat gct cac 3552 Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser Gly Ser Asp Ala His 1170 1175 1180 atc tca tca agt ggc att gtt cag gga cat gcg tac tca att ttg cag 3600 Ile Ser Ser Ser Gly Ile Val Gln Gly His Ala Tyr Ser Ile Leu Gln 1185 1190 1195 1200 gta aga gaa gtt gat ggc cac aaa ctc atc caa atc aga aat cca tgg 3648 Val Arg Glu Val Asp Gly His Lys Leu Ile Gln Ile Arg Asn Pro Trp 1205 1210 1215 gca aat gaa gtt gaa tgg aat gga cca tgg tca gac tcg tca cca gag 3696 Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser Asp Ser Ser Pro Glu 1220 1225 1230 tgg acg gaa cgg atg aag cat aag ctc atg cat gtt cca cag tcg aag 3744 Trp Thr Glu Arg Met Lys His Lys Leu Met His Val Pro Gln Ser Lys 1235 1240 1245 aat ggg gta ttc tgg atg tct tgg caa gat ttt cag att cac ttt cgg 3792 Asn Gly Val Phe Trp Met Ser Trp Gln Asp Phe Gln Ile His Phe Arg 1250 1255 1260 tca ata tat gtt tgt cgt gtt tat cca cct gag atg cgt tac tct gtc 3840 Ser Ile Tyr Val Cys Arg Val Tyr Pro Pro Glu Met Arg Tyr Ser Val 1265 1270 1275 1280 cat ggg caa tgg cgt ggc tac aat gca ggt ggt tgc caa gat tat gac 3888 His Gly Gln Trp Arg Gly Tyr Asn Ala Gly Gly Cys Gln Asp Tyr Asp 1285 1290 1295 tcg tgg cac caa aat cca cag tat cga ctt aga gta aca gga cgt gat 3936 Ser Trp His Gln Asn Pro Gln Tyr Arg Leu Arg Val Thr Gly Arg Asp 1300 1305 1310 gca cta tac cct gtt cac gtt ttt att acc ctt act cag ggt gtt ggt 3984 Ala Leu Tyr Pro Val His Val Phe Ile Thr Leu Thr Gln Gly Val Gly 1315 1320 1325 ttc tct aga aag acg aat ggt ttt cgg aac tac caa tct agc cat gat 4032 Phe Ser Arg Lys Thr Asn Gly Phe Arg Asn Tyr Gln Ser Ser His Asp 1330 1335 1340 tct tca atg ttt tac att gga atg agg ata ctc aag aca cag ggc tgc 4080 Ser Ser Met Phe Tyr Ile Gly Met Arg Ile Leu Lys Thr Gln Gly Cys 1345 1350 1355 1360 cgt gct gct tac aat atc tac atg cat gaa agc gct ggt gga aca gat 4128 Arg Ala Ala Tyr Asn Ile Tyr Met His Glu Ser Ala Gly Gly Thr Asp 1365 1370 1375 tac gtt aac tcg agg gag ata tca tgc gaa ctg gtc ttg gat cct tat 4176 Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Leu Val Leu Asp Pro Tyr 1380 1385 1390 ccc aaa ggg tac aca att gtg cca act acc atc cac cct ggg gag gaa 4224 Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile His Pro Gly Glu Glu 1395 1400 1405 gca cct ttt gtt ttg tca gtt ttt tca aaa gca tca atc aga cta gag 4272 Ala Pro Phe Val Leu Ser Val Phe Ser Lys Ala Ser Ile Arg Leu Glu 1410 1415 1420 gct gtt tag ttc aag att gag atc cca tgt gtt tga tgg tag ctg cgt 4320 Ala Val * Phe Lys Ile Glu Ile Pro Cys Val * Trp * Leu Arg 1425 1430 1435 ctg ctg ggc acc cgt gca cgc agg atc cag ctg tgg gtt ctc ggg aac 4368 Leu Leu Gly Thr Arg Ala Arg Arg Ile Gln Leu Trp Val Leu Gly Asn 1440 1445 1450 tag ata atg ggt ata gga att gcc tcc tgg aca act tca atc aat ctt 4416 * Ile Met Gly Ile Gly Ile Ala Ser Trp Thr Thr Ser Ile Asn Leu 1455 1460 1465 gct gca tgc aag tac cta agt tcg gtt gct tgt tgc aga tct gac aaa 4464 Ala Ala Cys Lys Tyr Leu Ser Ser Val Ala Cys Cys Arg Ser Asp Lys 1470 1475 1480 cgg caa tgc ttc ttg tgc tga agg gaa agg aga gaa ggc atg atc cat 4512 Arg Gln Cys Phe Leu Cys * Arg Glu Arg Arg Glu Gly Met Ile His 1485 1490 1495 ggt tct ttg gta gct gcg caa agt gca ggg tga gag gct tgg ttc aat 4560 Gly Ser Leu Val Ala Ala Gln Ser Ala Gly * Glu Ala Trp Phe

Asn 1500 1505 1510 gtt tgt aga tag ccg tgg taa ctg acc tgg tag ccc atc cta tgt ata 4608 Val Cys Arg * Pro Trp * Leu Thr Trp * Pro Ile Leu Cys Ile 1515 1520 1525 ggt gtc ccg ttt acc ctg taa atg cta tag agt tag gtt agg tag cct 4656 Gly Val Pro Phe Thr Leu * Met Leu * Ser * Val Arg * Pro 1530 1535 gtc gtt cct gtt aac gca tag ggc tct tat gca gct gtg aaa tgt ctt 4704 Val Val Pro Val Asn Ala * Gly Ser Tyr Ala Ala Val Lys Cys Leu 1540 1545 1550 gtt ggc aag ctg cag ttt tgc tga ttt gag cgt gga gta gtc ggc cat 4752 Val Gly Lys Leu Gln Phe Cys * Phe Glu Arg Gly Val Val Gly His 1555 1560 1565 agc tgt tcc cat tgg ttt gcc ctg tat gta atc gga atc tga tgt cat 4800 Ser Cys Ser His Trp Phe Ala Leu Tyr Val Ile Gly Ile * Cys His 1570 1575 1580 tca atg aac cta ttt ttt ggg tgc cat gcg aag ctg tct aaa aaa aaa 4848 Ser Met Asn Leu Phe Phe Gly Cys His Ala Lys Leu Ser Lys Lys Lys 1585 1590 1595 1600 aaa aaa aaa 4857 Lys Lys Lys 2 1603 PRT Zea mays 2 Leu Ser Phe Ala Val Pro Ile Trp Ile Arg Asn Gly Tyr Ser Phe Trp 1 5 10 15 Ile Pro Gly Arg Glu Phe Ala Asn Arg Glu Asn Val Ser Gln Ala Pro 20 25 30 Gly Glu Lys Glu Arg Ala Leu Phe Val Ile Thr Ile Ala Val Phe Thr 35 40 45 Ala Ser Ile Ile Gly Leu Gly Ala Ile Val Ser Ala Lys Pro Leu Asp 50 55 60 Ala Leu Gly Tyr Lys Gly Trp Asp Ala Asp Lys Asn Ser Ser Tyr Ser 65 70 75 80 Pro Tyr Ala Thr Ser Met Tyr Leu Gly Trp Ala Leu Ser Ser Thr Ile 85 90 95 Ala Val Ile Thr Thr Gly Leu Ile Pro Ile Val Ala Trp Phe Ala Thr 100 105 110 Tyr Arg Phe Ser Pro Ser Ser Ala Ile Cys Val Gly Leu Phe Ala Thr 115 120 125 Val Leu Val Ser Phe Cys Gly Ala Ser Tyr Trp Gly Val Val Asn Ser 130 135 140 Arg Glu Asp Gly Val Pro Leu Lys Ala Asp Phe Leu Ala Ala Leu Leu 145 150 155 160 Pro Leu Leu Cys Ile Pro Ala Phe Phe Ser Leu Phe Thr Gly Leu Tyr 165 170 175 Lys Trp Lys Asp Asp Asp Trp Lys Ile Ser Arg Gly Val Tyr Leu Phe 180 185 190 Val Gly Met Gly Met Leu Leu Leu Phe Gly Ala Val Ala Ala Val Ile 195 200 205 Val Thr Ile Arg Pro Trp Thr Val Gly Val Ala Cys Leu Val Ala Ile 210 215 220 Leu Phe Leu Val Phe Val Ile Gly Val Ile His Tyr Trp Thr Ser Asn 225 230 235 240 Asn Phe Tyr Leu Thr Arg Thr Gln Met Leu Leu Val Cys Ser Ile Ala 245 250 255 Phe Leu Leu Ala Leu Ala Ala Phe Leu Met Gly Leu Phe His Gly Lys 260 265 270 Pro Phe Val Gly Ala Ser Ile Gly Tyr Phe Ser Phe Ile Phe Leu Leu 275 280 285 Thr Gly Arg Ala Leu Thr Val Leu Leu Ser Pro Pro Ile Val Val Tyr 290 295 300 Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His Ala Asp Ser 305 310 315 320 Ala Lys Asn Val Ser Tyr Ala Phe Leu Ile Leu Tyr Gly Ile Ala Leu 325 330 335 Ala Thr Glu Val Trp Gly Val Ile Ala Ser Leu Ile Met Asn Pro Pro 340 345 350 Phe Val Gly Ala Gly Val Ser Ala Thr Thr Leu Val Ile Ala Phe Ser 355 360 365 Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Lys Met Met Glu Asp Ala 370 375 380 Val His Phe Leu Ser Lys Asp Thr Val Val Gln Ala Met Ser Arg Ser 385 390 395 400 Ala Asn Lys Thr Arg Asn Ala Ile Ser Gly Thr Tyr Ser Ala Pro Gln 405 410 415 Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro Ala Leu Thr 420 425 430 Leu Asp Arg Ala Gly Asn Phe Val Leu Pro Arg Ala Asp Val Met Lys 435 440 445 Leu Arg Asp Arg Leu Arg Asn Glu Glu Ile Ala Ala Gly Ser Phe Leu 450 455 460 Cys Gly Val Lys Asp Cys Leu Leu Ile Cys Pro Gln Ser Leu Ser Asn 465 470 475 480 Ile Asp Tyr Arg Arg Asn Met Cys Ala His Ala Arg Ile Leu Ala Leu 485 490 495 Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp Lys Phe Gly 500 505 510 Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala Glu Gln Ile 515 520 525 Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile Gly Leu Ser 530 535 540 Asp Leu Ser Ala Lys Glu Ile Lys Lys Trp Met Pro Glu Asp Arg Arg 545 550 555 560 Gln Phe Glu Leu Ile Gln Glu Ser Tyr Ile Arg Glu Lys Glu Met Glu 565 570 575 Glu Glu Ala Leu Met Gln Arg Arg Glu Glu Glu Gly Lys Gly Arg Glu 580 585 590 Arg Arg Arg Ala Leu Leu Glu Arg Glu Glu Arg Lys Trp Lys Glu Leu 595 600 605 Glu Ile Ser Leu Leu Ser Ser Ile Pro Asn Thr Gly Ser Arg Asp Ala 610 615 620 Ala Ala Met Ala Ala Ala Val Arg Ala Val Gly Gly Asp Ser Ala Leu 625 630 635 640 Glu Asp Ser Phe Ala Arg Asp Arg Val Ser Ser Ile Ala Asn His Ile 645 650 655 Arg Lys Ala Gln Leu Ala Arg Arg Ala Glu Gln Thr Gly Ile Pro Gly 660 665 670 Thr Ile Cys Ile Leu Asp Asp Glu Pro Arg Ser Thr Gly Arg His Cys 675 680 685 Gly Glu Leu Asp Leu Cys Leu Cys Gln Ser Gln Lys Val Thr Leu Ser 690 695 700 Ile Ala Val Met Val Gln Pro Val Ser Gly Pro Val Cys Leu Phe Gly 705 710 715 720 Ser Glu Phe Gln Lys Val Cys Trp Glu Ile Leu Val Ala Gly Ser Glu 725 730 735 Gln Gly Met Glu Ala Gly Gln Val Gly Leu Arg Leu Val Thr Lys Gly 740 745 750 Glu Arg Met Thr Thr Val Ala Lys Glu Trp Asn Ile Gly Ala Ser Ser 755 760 765 Ile Ala Asp Gly Arg Trp His Leu Val Thr Val Thr Leu Asp Ala Asp 770 775 780 Leu Gly Glu Ala Thr Ser Phe Ile Asp Gly Val Tyr Asp Gly Tyr Gln 785 790 795 800 Asn Gly Leu Pro Leu Pro Thr Asp Asn Gly Ile Trp Glu Pro Gly Thr 805 810 815 Asp Ile Trp Val Gly Ala Arg Pro Pro Met Asp Leu Asp Ala Phe Gly 820 825 830 Arg Ser Asp Ser Glu Gly Ser Asp Ser Lys Met Gln Ile Met Asp Ala 835 840 845 Phe Leu Trp Gly Arg Cys Leu Ser Glu Asp Glu Val Thr Val Leu His 850 855 860 Thr Ala Met Ser Pro Ala Glu Tyr Gly Phe Phe Asp Leu Ala Pro Gly 865 870 875 880 Asp Ala Trp His Gly Ser Tyr Ser Ala Arg Val Asp Asp Trp Glu Ser 885 890 895 Glu Glu Ala Tyr Glu Leu Tyr Asp Gln Gly Asp Val Glu Trp Asp Gly 900 905 910 Gln Tyr Ser Ser Gly Arg Lys Arg Pro Val His Asp Ala Val Ala Ile 915 920 925 Asp Leu Asp Ser Phe Ala Arg Arg Pro Arg Lys Pro Arg Phe Glu Thr 930 935 940 Arg Asp Glu Val Asn Gln Arg Met Leu Ser Val Glu Arg Ala Val Arg 945 950 955 960 Asp Ala Leu Ile Ala Lys Gly Glu Arg Asn Phe Thr Asp Gln Glu Phe 965 970 975 Pro Pro Glu Asp Arg Ser Leu Phe Val Asp Pro Met Asn Pro Pro Leu 980 985 990 Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro Ser Asp Ile Ala Lys 995 1000 1005 Asp Ile Ser Ile Ser Cys Gln Pro Cys Leu Phe Ser Gly Ser Val Asn 1010 1015 1020 Ser Ser Asp Val Cys Gln Gly Arg Leu Gly Asp Cys Trp Phe Leu Ser 1025 1030 1035 1040 Ala Val Ala Val Leu Thr Glu Met Ser Arg Ile Ser Glu Val Ile Ile 1045 1050 1055 Thr Pro Glu Tyr Asn Asp Glu Gly Ile Tyr Thr Val Arg Phe Cys Ile 1060 1065 1070 Gln Gly Glu Trp Val Ala Val Val Val Asp Asp Trp Ile Pro Cys Glu 1075 1080 1085 Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg Lys Gln Asn Glu Leu 1090 1095 1100 Trp Val Ser Ile Leu Glu Lys Ala Tyr Ala Lys Leu His Gly Ser Tyr 1105 1110 1115 1120 Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala Leu Val Asp Leu Thr 1125 1130 1135 Gly Gly Ala Gly Glu Glu Ile Asp Met Arg Ser Pro Gln Ala Gln Leu 1140 1145 1150 Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu Leu His Phe Lys Gln 1155 1160 1165 Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser Gly Ser Asp Ala His 1170 1175 1180 Ile Ser Ser Ser Gly Ile Val Gln Gly His Ala Tyr Ser Ile Leu Gln 1185 1190 1195 1200 Val Arg Glu Val Asp Gly His Lys Leu Ile Gln Ile Arg Asn Pro Trp 1205 1210 1215 Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser Asp Ser Ser Pro Glu 1220 1225 1230 Trp Thr Glu Arg Met Lys His Lys Leu Met His Val Pro Gln Ser Lys 1235 1240 1245 Asn Gly Val Phe Trp Met Ser Trp Gln Asp Phe Gln Ile His Phe Arg 1250 1255 1260 Ser Ile Tyr Val Cys Arg Val Tyr Pro Pro Glu Met Arg Tyr Ser Val 1265 1270 1275 1280 His Gly Gln Trp Arg Gly Tyr Asn Ala Gly Gly Cys Gln Asp Tyr Asp 1285 1290 1295 Ser Trp His Gln Asn Pro Gln Tyr Arg Leu Arg Val Thr Gly Arg Asp 1300 1305 1310 Ala Leu Tyr Pro Val His Val Phe Ile Thr Leu Thr Gln Gly Val Gly 1315 1320 1325 Phe Ser Arg Lys Thr Asn Gly Phe Arg Asn Tyr Gln Ser Ser His Asp 1330 1335 1340 Ser Ser Met Phe Tyr Ile Gly Met Arg Ile Leu Lys Thr Gln Gly Cys 1345 1350 1355 1360 Arg Ala Ala Tyr Asn Ile Tyr Met His Glu Ser Ala Gly Gly Thr Asp 1365 1370 1375 Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Leu Val Leu Asp Pro Tyr 1380 1385 1390 Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile His Pro Gly Glu Glu 1395 1400 1405 Ala Pro Phe Val Leu Ser Val Phe Ser Lys Ala Ser Ile Arg Leu Glu 1410 1415 1420 Ala Val Phe Lys Ile Glu Ile Pro Cys Val Trp Leu Arg Leu Leu Gly 1425 1430 1435 1440 Thr Arg Ala Arg Arg Ile Gln Leu Trp Val Leu Gly Asn Ile Met Gly 1445 1450 1455 Ile Gly Ile Ala Ser Trp Thr Thr Ser Ile Asn Leu Ala Ala Cys Lys 1460 1465 1470 Tyr Leu Ser Ser Val Ala Cys Cys Arg Ser Asp Lys Arg Gln Cys Phe 1475 1480 1485 Leu Cys Arg Glu Arg Arg Glu Gly Met Ile His Gly Ser Leu Val Ala 1490 1495 1500 Ala Gln Ser Ala Gly Glu Ala Trp Phe Asn Val Cys Arg Pro Trp Leu 1505 1510 1515 1520 Thr Trp Pro Ile Leu Cys Ile Gly Val Pro Phe Thr Leu Met Leu Ser 1525 1530 1535 Val Arg Pro Val Val Pro Val Asn Ala Gly Ser Tyr Ala Ala Val Lys 1540 1545 1550 Cys Leu Val Gly Lys Leu Gln Phe Cys Phe Glu Arg Gly Val Val Gly 1555 1560 1565 His Ser Cys Ser His Trp Phe Ala Leu Tyr Val Ile Gly Ile Cys His 1570 1575 1580 Ser Met Asn Leu Phe Phe Gly Cys His Ala Lys Leu Ser Lys Lys Lys 1585 1590 1595 1600 Lys Lys Lys 3 6467 DNA Arabidopsis CDS (120)...(6465) Arabidopsis calpain 3 cttggttggt tttaagctgc ggatttgatg atttgtgcgc aagcttgggg tttcagcttt 60 tttttgtgat ggaattttga tttccgagtt gcatggtgtt gtaggtggga gaagaagcc 119 atg gaa ggg gat gag cga gga gtc tta ctt gct tgt gta att tcg ggt 167 Met Glu Gly Asp Glu Arg Gly Val Leu Leu Ala Cys Val Ile Ser Gly 1 5 10 15 acc ctt ttc acg gtt ttc ggt tcg ggt tcg ttt tgg ata ctt tgg gct 215 Thr Leu Phe Thr Val Phe Gly Ser Gly Ser Phe Trp Ile Leu Trp Ala 20 25 30 gtt aat tgg cgg cca tgg cgt ctc tac agt tgg atc ttt gct aga aaa 263 Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Phe Ala Arg Lys 35 40 45 tgg cca aaa gta ttg caa ggt cct cag ctt gat ata cta tgt ggt gtt 311 Trp Pro Lys Val Leu Gln Gly Pro Gln Leu Asp Ile Leu Cys Gly Val 50 55 60 cta tct ctt ttt gct tgg att gtg gta gta tcc cct att gca atc ttg 359 Leu Ser Leu Phe Ala Trp Ile Val Val Val Ser Pro Ile Ala Ile Leu 65 70 75 80 ata gga tgg ggt tct tgg ctg att gtg ata ttg gat cga cat atc att 407 Ile Gly Trp Gly Ser Trp Leu Ile Val Ile Leu Asp Arg His Ile Ile 85 90 95 ggg ctg gcg ata ata atg gct gga aca gcc ctt tta ctg gca ttc tac 455 Gly Leu Ala Ile Ile Met Ala Gly Thr Ala Leu Leu Leu Ala Phe Tyr 100 105 110 tca atc atg ctt tgg tgg agg acc cag tgg caa agc tca aga gct gtc 503 Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Arg Ala Val 115 120 125 gct tta ctt ctc ctt ctt ggt gtt gcc tta cta tgt gcg tat gaa ctc 551 Ala Leu Leu Leu Leu Leu Gly Val Ala Leu Leu Cys Ala Tyr Glu Leu 130 135 140 tgt gct gtc tat gtt acg gct ggt gcg cat gca tct cag caa tat tct 599 Cys Ala Val Tyr Val Thr Ala Gly Ala His Ala Ser Gln Gln Tyr Ser 145 150 155 160 cct tct ggt ttc ttt ttc ggt gta tca gca atc gcg ttg gca att aac 647 Pro Ser Gly Phe Phe Phe Gly Val Ser Ala Ile Ala Leu Ala Ile Asn 165 170 175 atg cta ttt atc tgc cgc atg gtc ttt aat gga aat ggt tta gat gtg 695 Met Leu Phe Ile Cys Arg Met Val Phe Asn Gly Asn Gly Leu Asp Val 180 185 190 gac gaa tat gta agg agg gca tat aaa ttt gct tat tca gat tgt ata 743 Asp Glu Tyr Val Arg Arg Ala Tyr Lys Phe Ala Tyr Ser Asp Cys Ile 195 200 205 gaa gtg ggt cct gtg gct tgt ttg cct gaa cct cct gat cct aat gaa 791 Glu Val Gly Pro Val Ala Cys Leu Pro Glu Pro Pro Asp Pro Asn Glu 210 215 220 tta tat ccc cgg caa acc agc agg gct tca cat ctt ggc ctt ctg tac 839 Leu Tyr Pro Arg Gln Thr Ser Arg Ala Ser His Leu Gly Leu Leu Tyr 225 230 235 240 ctg ggc tca ctc gta gtt ctc ctt gcc tac tca gtc cta tat ggt ctc 887 Leu Gly Ser Leu Val Val Leu Leu Ala Tyr Ser Val Leu Tyr Gly Leu 245 250 255 aca gct agg gaa tca cgt tgg ctt gga gga atc aca tca gct gca gtt 935 Thr Ala Arg Glu Ser Arg Trp Leu Gly Gly Ile Thr Ser Ala Ala Val 260 265 270 att gtt ctt gac tgg aat att ggg gca tgc ttg tat ggg ttt aag ctt 983 Ile Val Leu Asp Trp Asn Ile Gly Ala Cys Leu Tyr Gly Phe Lys Leu 275 280 285 ctt cag aat cgt gtt ctg gca ctt ttt gtt gct ggc ata tcc cgt ctt 1031 Leu Gln Asn Arg Val Leu Ala Leu Phe Val Ala Gly Ile Ser Arg Leu 290 295 300 ttc cta ata tgt ttt ggc ata cac tac tgg tac cta ggg cat tgt att 1079 Phe Leu Ile Cys Phe Gly Ile His Tyr Trp Tyr Leu Gly His Cys Ile 305 310 315 320 agt tac att ttc gta gca tca gtt cta tca ggt gct gct gtt tct cgg 1127 Ser Tyr Ile Phe Val Ala Ser Val Leu Ser Gly Ala Ala Val Ser Arg 325 330 335 cat cta tct ata aca gac cca tca gct gca aga aga gat gcc tta cag 1175 His Leu Ser Ile Thr Asp Pro Ser Ala Ala Arg Arg Asp Ala Leu Gln 340 345 350 agc aca gtg atc cgc ttg aga gaa ggt ttt cgg aga aaa gag cag aat 1223 Ser Thr Val Ile Arg Leu Arg Glu Gly Phe Arg Arg Lys Glu Gln Asn 355 360 365 agt tct tca agt tct tca gat ggt tgt ggc tca agt ata aaa aga agt 1271 Ser Ser Ser Ser Ser Ser Asp Gly Cys Gly Ser Ser Ile Lys Arg Ser 370 375 380 agt agt atc gat gct ggc cat act ggt tgt act aat gaa gca aat cgt 1319 Ser Ser Ile Asp Ala Gly His Thr Gly Cys Thr Asn Glu Ala Asn Arg 385

390 395 400 acg gca gaa tct tgc acg gct gac aat cta act cga aca ggc agc tct 1367 Thr Ala Glu Ser Cys Thr Ala Asp Asn Leu Thr Arg Thr Gly Ser Ser 405 410 415 cag gag gga atc aat agc gac aaa agc gaa gaa agt gga aga cca agc 1415 Gln Glu Gly Ile Asn Ser Asp Lys Ser Glu Glu Ser Gly Arg Pro Ser 420 425 430 tta ggt tta cgt agt agt tca tgt cgt tct gtg gtc caa gag ccc gaa 1463 Leu Gly Leu Arg Ser Ser Ser Cys Arg Ser Val Val Gln Glu Pro Glu 435 440 445 gca gga acg tct tat ttt atg gac aaa gtt tct gat caa aat aac act 1511 Ala Gly Thr Ser Tyr Phe Met Asp Lys Val Ser Asp Gln Asn Asn Thr 450 455 460 ctt gtt gtt tgt tcg agc agt ggt cta gat agc caa ggt tac gag tct 1559 Leu Val Val Cys Ser Ser Ser Gly Leu Asp Ser Gln Gly Tyr Glu Ser 465 470 475 480 agc aca tcg aat tct gca aac cag cag ctt ttg gat atg aat ttg gct 1607 Ser Thr Ser Asn Ser Ala Asn Gln Gln Leu Leu Asp Met Asn Leu Ala 485 490 495 ctt gct ttc cag gac cag tta aac aat cct agg ata gcc tcg ata ctt 1655 Leu Ala Phe Gln Asp Gln Leu Asn Asn Pro Arg Ile Ala Ser Ile Leu 500 505 510 aag aag aaa gca aaa gaa ggt gat ctt gaa ctg act aat ttg ctg caa 1703 Lys Lys Lys Ala Lys Glu Gly Asp Leu Glu Leu Thr Asn Leu Leu Gln 515 520 525 gac aag ggg ttg gac cct aac ttt gct gta atg ttg aag gaa aaa aac 1751 Asp Lys Gly Leu Asp Pro Asn Phe Ala Val Met Leu Lys Glu Lys Asn 530 535 540 ttg gat cct act ata ttg gca cta ctt cag agg agt agt ttg gat gca 1799 Leu Asp Pro Thr Ile Leu Ala Leu Leu Gln Arg Ser Ser Leu Asp Ala 545 550 555 560 gat aga gat cac cgc gac aat act gat att aca atc att gac tca aac 1847 Asp Arg Asp His Arg Asp Asn Thr Asp Ile Thr Ile Ile Asp Ser Asn 565 570 575 agt gtt gac aat act ttg cca aat cag att tct tta tcc gaa gaa ttg 1895 Ser Val Asp Asn Thr Leu Pro Asn Gln Ile Ser Leu Ser Glu Glu Leu 580 585 590 aga ctc cgt gga cta gag aag tgg ctt aag ttg tct aga ctt ctt ctg 1943 Arg Leu Arg Gly Leu Glu Lys Trp Leu Lys Leu Ser Arg Leu Leu Leu 595 600 605 cac cat gta gcg ggg aca cca gag aga gca tgg ggc ctc ttt agt ctt 1991 His His Val Ala Gly Thr Pro Glu Arg Ala Trp Gly Leu Phe Ser Leu 610 615 620 gtc ttt atc ctt gaa aca atc att gtg gcc att ttt cgc cca aag acc 2039 Val Phe Ile Leu Glu Thr Ile Ile Val Ala Ile Phe Arg Pro Lys Thr 625 630 635 640 atc acg att ata aat tct agt cat caa cag ttc gaa ttt ggt ttc tct 2087 Ile Thr Ile Ile Asn Ser Ser His Gln Gln Phe Glu Phe Gly Phe Ser 645 650 655 gtg ctg cta ttg tca cct gtt gtc tgt tca ata atg gct ttt ctt cgg 2135 Val Leu Leu Leu Ser Pro Val Val Cys Ser Ile Met Ala Phe Leu Arg 660 665 670 tct ctt caa gtt gag gaa atg gcc ttg aca tca aaa tct cgc aag tat 2183 Ser Leu Gln Val Glu Glu Met Ala Leu Thr Ser Lys Ser Arg Lys Tyr 675 680 685 ggc ttt gtt gcc tgg ctt ctg agc aca tca gtt gga ttg tca ctc tcg 2231 Gly Phe Val Ala Trp Leu Leu Ser Thr Ser Val Gly Leu Ser Leu Ser 690 695 700 ttc ttg agt aaa tcg tca gta ctt ctg gga ata tcc ttg act gtg ccc 2279 Phe Leu Ser Lys Ser Ser Val Leu Leu Gly Ile Ser Leu Thr Val Pro 705 710 715 720 ctc atg gca gca tgc ctg tct att gct gtt ccc ata tgg atg cat aat 2327 Leu Met Ala Ala Cys Leu Ser Ile Ala Val Pro Ile Trp Met His Asn 725 730 735 ggg tac caa ttt tgg gtt cca cag tta tca tgt ggt gac cag gca aga 2375 Gly Tyr Gln Phe Trp Val Pro Gln Leu Ser Cys Gly Asp Gln Ala Arg 740 745 750 gat tta cga tct ccc agg ata aag ggg ttt att ctt tgg att tgt gtt 2423 Asp Leu Arg Ser Pro Arg Ile Lys Gly Phe Ile Leu Trp Ile Cys Val 755 760 765 gtg ttg ttt gcg ggt tct gta att tct ctt ggt gcg att ata tct gct 2471 Val Leu Phe Ala Gly Ser Val Ile Ser Leu Gly Ala Ile Ile Ser Ala 770 775 780 aaa cct ttg gat gat tta aag tat aag ctg ttt agt gcc aga gaa aac 2519 Lys Pro Leu Asp Asp Leu Lys Tyr Lys Leu Phe Ser Ala Arg Glu Asn 785 790 795 800 aac gtc acg tca cca tat aca tct tct gta tac ctt ggt tgg gca atg 2567 Asn Val Thr Ser Pro Tyr Thr Ser Ser Val Tyr Leu Gly Trp Ala Met 805 810 815 tca tct gga att gct tta gta gtt acc gcc att cta cca ata gtt tca 2615 Ser Ser Gly Ile Ala Leu Val Val Thr Ala Ile Leu Pro Ile Val Ser 820 825 830 tgg ttt gca act tat agg ttt tcc cac tct tct gct gtc tgt ctc atg 2663 Trp Phe Ala Thr Tyr Arg Phe Ser His Ser Ser Ala Val Cys Leu Met 835 840 845 ata ttc tca gtt gtt ctc gtg gca ttt tgt gga act tca tat ttg gaa 2711 Ile Phe Ser Val Val Leu Val Ala Phe Cys Gly Thr Ser Tyr Leu Glu 850 855 860 gtt gta aaa tct aga gat gat cag ttg ccc aca aag ggt gat ttc ctt 2759 Val Val Lys Ser Arg Asp Asp Gln Leu Pro Thr Lys Gly Asp Phe Leu 865 870 875 880 gcg gcc ttg ctt cca ctt gca tgc att ccg gcg ctg ctt tca cta tgc 2807 Ala Ala Leu Leu Pro Leu Ala Cys Ile Pro Ala Leu Leu Ser Leu Cys 885 890 895 tgt ggg atg gtt aaa tgg aag gac gat tgt tgg ata ctc tct cga ggt 2855 Cys Gly Met Val Lys Trp Lys Asp Asp Cys Trp Ile Leu Ser Arg Gly 900 905 910 gta tat gtt ttc ttt tca ata ggt ctt ctt ctt ctt ttt ggt gcg ata 2903 Val Tyr Val Phe Phe Ser Ile Gly Leu Leu Leu Leu Phe Gly Ala Ile 915 920 925 gca gct gtc att gca gtc aaa cca tgg acg ata ggc gta tct ttt ctc 2951 Ala Ala Val Ile Ala Val Lys Pro Trp Thr Ile Gly Val Ser Phe Leu 930 935 940 tta gtt ctt ttc ctt atg gtg gta aca att ggt gta atc cat ctt tgg 2999 Leu Val Leu Phe Leu Met Val Val Thr Ile Gly Val Ile His Leu Trp 945 950 955 960 gcg tca aac aat ttc tat tta acc agg aaa cag aca tcc ttt gtc tgc 3047 Ala Ser Asn Asn Phe Tyr Leu Thr Arg Lys Gln Thr Ser Phe Val Cys 965 970 975 ttt ctt gct ctt ctt ttg ggt ttg gcc gca ttc ctt ctc gga tgg cat 3095 Phe Leu Ala Leu Leu Leu Gly Leu Ala Ala Phe Leu Leu Gly Trp His 980 985 990 caa gat aaa gca ttt gct gga gca tct gtt ggt tac ttt aca ttc ctg 3143 Gln Asp Lys Ala Phe Ala Gly Ala Ser Val Gly Tyr Phe Thr Phe Leu 995 1000 1005 tct ctg ttg gct gga aga gca tta gct gtt ctt cta tcc cca cca att 3191 Ser Leu Leu Ala Gly Arg Ala Leu Ala Val Leu Leu Ser Pro Pro Ile 1010 1015 1020 gta gta tat tct cca agg gtg cta cca gta tat gtc tac gat gct cat 3239 Val Val Tyr Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His 1025 1030 1035 1040 gct gat tgc gga aag aat gtc agt gct gca ttt ctt gtc ctg tat gga 3287 Ala Asp Cys Gly Lys Asn Val Ser Ala Ala Phe Leu Val Leu Tyr Gly 1045 1050 1055 att gct ttg gca aca gaa ggc tgg ggt gtt gtt gct agt ctg ata att 3335 Ile Ala Leu Ala Thr Glu Gly Trp Gly Val Val Ala Ser Leu Ile Ile 1060 1065 1070 tat cct ccg ttt gcg ggt gct gct gta tca gct atc acc ctt gta gta 3383 Tyr Pro Pro Phe Ala Gly Ala Ala Val Ser Ala Ile Thr Leu Val Val 1075 1080 1085 gcc ttt ggg ttt gct gtt tct cgc cca tgt ttg act ctt gag atg atg 3431 Ala Phe Gly Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Glu Met Met 1090 1095 1100 gag gtt gct gta cgc ttt ctt agc aag gat act ata gtg caa gct atc 3479 Glu Val Ala Val Arg Phe Leu Ser Lys Asp Thr Ile Val Gln Ala Ile 1105 1110 1115 1120 tct cga tct gcc acg aaa aca aga aat gct cta tcc ggc acg tat tca 3527 Ser Arg Ser Ala Thr Lys Thr Arg Asn Ala Leu Ser Gly Thr Tyr Ser 1125 1130 1135 gct ccc caa agg tcc gcc agc tct gca gct ctt ctg gtt ggg gat ccc 3575 Ala Pro Gln Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro 1140 1145 1150 tct gca atg cgt gat aaa gca ggg aac ttt gtg ctt cct aga gat gat 3623 Ser Ala Met Arg Asp Lys Ala Gly Asn Phe Val Leu Pro Arg Asp Asp 1155 1160 1165 gtc atg aaa tta agg gat cgt ctc agg aac gaa gaa aga gtt gct gga 3671 Val Met Lys Leu Arg Asp Arg Leu Arg Asn Glu Glu Arg Val Ala Gly 1170 1175 1180 tca atc ttc tac aaa atg caa tgc agg aaa gga ttc cgt cat gaa cca 3719 Ser Ile Phe Tyr Lys Met Gln Cys Arg Lys Gly Phe Arg His Glu Pro 1185 1190 1195 1200 cct aca aat gta gat tat aga aga gac atg tgt gcc cat gca aga gtt 3767 Pro Thr Asn Val Asp Tyr Arg Arg Asp Met Cys Ala His Ala Arg Val 1205 1210 1215 ttg gca ctg gaa gag gca att gat aca gaa tgg gtg tat atg tgg gac 3815 Leu Ala Leu Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp 1220 1225 1230 aaa ttt ggt ggt tat tta cta cta ttg tta ggt ttg aca gct aag gcg 3863 Lys Phe Gly Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala 1235 1240 1245 gag aga gtt cag gat gag gta cgg ttg cgg ctc ttc tta gat agc att 3911 Glu Arg Val Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile 1250 1255 1260 ggg ttc tcg gat tta agt gcc aga aaa atc agt aaa tgg aag cca gag 3959 Gly Phe Ser Asp Leu Ser Ala Arg Lys Ile Ser Lys Trp Lys Pro Glu 1265 1270 1275 1280 gat aga aga caa ttc gaa att att caa gag agt tat ctg aga gag aaa 4007 Asp Arg Arg Gln Phe Glu Ile Ile Gln Glu Ser Tyr Leu Arg Glu Lys 1285 1290 1295 gag atg gaa gag gaa agc ctt atg cag aga cgt gaa gaa gaa ggg aga 4055 Glu Met Glu Glu Glu Ser Leu Met Gln Arg Arg Glu Glu Glu Gly Arg 1300 1305 1310 ggt aaa gaa aga agg aaa gct ctt ttg gag aag gaa gag cgc aaa tgg 4103 Gly Lys Glu Arg Arg Lys Ala Leu Leu Glu Lys Glu Glu Arg Lys Trp 1315 1320 1325 aag gaa att gaa gcg tcc ctt att cca tct att cct aat gct ggt agc 4151 Lys Glu Ile Glu Ala Ser Leu Ile Pro Ser Ile Pro Asn Ala Gly Ser 1330 1335 1340 agg gag gca gca gcc atg gca gct gca ata cgt gct gtt ggg ggt gat 4199 Arg Glu Ala Ala Ala Met Ala Ala Ala Ile Arg Ala Val Gly Gly Asp 1345 1350 1355 1360 tct gtc ctt gag gat tcc ttc gca aga gag agg gtc tcg ggt att gca 4247 Ser Val Leu Glu Asp Ser Phe Ala Arg Glu Arg Val Ser Gly Ile Ala 1365 1370 1375 cgt agg ata cgc act gct caa cta gaa cga cgt gca caa cag act gga 4295 Arg Arg Ile Arg Thr Ala Gln Leu Glu Arg Arg Ala Gln Gln Thr Gly 1380 1385 1390 ata tct ggg gca gtt tgt gtt ctt gat gat gaa cca atg ata agt ggt 4343 Ile Ser Gly Ala Val Cys Val Leu Asp Asp Glu Pro Met Ile Ser Gly 1395 1400 1405 aaa cat tgc ggc caa atg gac tca agt gtc tgt caa agt cag aag att 4391 Lys His Cys Gly Gln Met Asp Ser Ser Val Cys Gln Ser Gln Lys Ile 1410 1415 1420 agc ttt tcc gtt aca gca atg atc caa tcc gat tct gga cct gta tgt 4439 Ser Phe Ser Val Thr Ala Met Ile Gln Ser Asp Ser Gly Pro Val Cys 1425 1430 1435 1440 ctt ttt ggc act gaa ttt caa aag aaa gta tgt tgg gag att ctg gtt 4487 Leu Phe Gly Thr Glu Phe Gln Lys Lys Val Cys Trp Glu Ile Leu Val 1445 1450 1455 gct ggt tct gag caa gga att gag gct ggc caa gtt ggg ctt agg ttg 4535 Ala Gly Ser Glu Gln Gly Ile Glu Ala Gly Gln Val Gly Leu Arg Leu 1460 1465 1470 ata aca aaa ggt gag agg cag aca acc gtt gct aga gag tgg tat att 4583 Ile Thr Lys Gly Glu Arg Gln Thr Thr Val Ala Arg Glu Trp Tyr Ile 1475 1480 1485 ggt gca acc agc ata act gat gga agg tgg cat aca gtg aca atc aca 4631 Gly Ala Thr Ser Ile Thr Asp Gly Arg Trp His Thr Val Thr Ile Thr 1490 1495 1500 att gat gct gat gcg ggg gaa gct act tgt tac ata gat ggt ggg ttt 4679 Ile Asp Ala Asp Ala Gly Glu Ala Thr Cys Tyr Ile Asp Gly Gly Phe 1505 1510 1515 1520 gat ggc tac cag aat ggg tta cct cta agt att ggc agt gcc att tgg 4727 Asp Gly Tyr Gln Asn Gly Leu Pro Leu Ser Ile Gly Ser Ala Ile Trp 1525 1530 1535 gaa caa gga gct gaa gtt tgg ttg ggt gtt agg cca cct ata gat gtt 4775 Glu Gln Gly Ala Glu Val Trp Leu Gly Val Arg Pro Pro Ile Asp Val 1540 1545 1550 gat gca ttc ggg aga tca gat agt gat ggc gtc gaa tca aag atg cat 4823 Asp Ala Phe Gly Arg Ser Asp Ser Asp Gly Val Glu Ser Lys Met His 1555 1560 1565 att atg gat gtt ttc ctt tgg ggg aaa tgc tta agt gaa gaa gag gcc 4871 Ile Met Asp Val Phe Leu Trp Gly Lys Cys Leu Ser Glu Glu Glu Ala 1570 1575 1580 gct tct ttg cat gca gcc att ggc atg gct gac tta gac atg att gat 4919 Ala Ser Leu His Ala Ala Ile Gly Met Ala Asp Leu Asp Met Ile Asp 1585 1590 1595 1600 ttg tct gat gac aat tgg caa tgg acg gat tca ccc ccc aga gtc gat 4967 Leu Ser Asp Asp Asn Trp Gln Trp Thr Asp Ser Pro Pro Arg Val Asp 1605 1610 1615 ggt tgg gat agt gat cct gcc gat gtt gat ctc tat gat agg gat gac 5015 Gly Trp Asp Ser Asp Pro Ala Asp Val Asp Leu Tyr Asp Arg Asp Asp 1620 1625 1630 gta gat tgg gat gga caa tat tcc agt ggg agg aaa aga aga tca ggt 5063 Val Asp Trp Asp Gly Gln Tyr Ser Ser Gly Arg Lys Arg Arg Ser Gly 1635 1640 1645 cgg gat ttt gta atg agt gtc gat tcc ttt gcc agg aga cac agg aaa 5111 Arg Asp Phe Val Met Ser Val Asp Ser Phe Ala Arg Arg His Arg Lys 1650 1655 1660 ccc agg atg gag aca caa gaa gat ata aat caa aga atg cgt tca gtt 5159 Pro Arg Met Glu Thr Gln Glu Asp Ile Asn Gln Arg Met Arg Ser Val 1665 1670 1675 1680 gag ttg gct gtc aaa gaa gct ctc tct gca cga ggt gat aag caa ttt 5207 Glu Leu Ala Val Lys Glu Ala Leu Ser Ala Arg Gly Asp Lys Gln Phe 1685 1690 1695 act gac cag gaa ttt cct cca aat gat cgc tct tta ttt gtg gat aca 5255 Thr Asp Gln Glu Phe Pro Pro Asn Asp Arg Ser Leu Phe Val Asp Thr 1700 1705 1710 caa aat ccc cca tca aaa ttg cag gtt gtt tct gaa tgg atg aga cct 5303 Gln Asn Pro Pro Ser Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro 1715 1720 1725 gac tcc att gtg aaa gaa aac ggt agt gat tcc cgt ccc tgc ctg ttc 5351 Asp Ser Ile Val Lys Glu Asn Gly Ser Asp Ser Arg Pro Cys Leu Phe 1730 1735 1740 tct ggg gat gca aat cct tca gat gtt tgc cag ggg cgt ttg ggg gat 5399 Ser Gly Asp Ala Asn Pro Ser Asp Val Cys Gln Gly Arg Leu Gly Asp 1745 1750 1755 1760 tgt tgg ttc tta agc gcc gtt gca gtt ttg aca gag gtt tca cga ata 5447 Cys Trp Phe Leu Ser Ala Val Ala Val Leu Thr Glu Val Ser Arg Ile 1765 1770 1775 tct gaa gtg atc att act cct gaa tac aac gag gaa ggg atc tac act 5495 Ser Glu Val Ile Ile Thr Pro Glu Tyr Asn Glu Glu Gly Ile Tyr Thr 1780 1785 1790 gtt cgt ttt tgt att cag ggt gag tgg gtt cct gtt gtt atc gat gac 5543 Val Arg Phe Cys Ile Gln Gly Glu Trp Val Pro Val Val Ile Asp Asp 1795 1800 1805 tgg att cca tgt gaa tca cct ggt aaa cca gct ttt gct act agc aga 5591 Trp Ile Pro Cys Glu Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg 1810 1815 1820 aag ctc aat gaa ctc tgg gtc tcc atg gtg gag aaa gca tat gcc aag 5639 Lys Leu Asn Glu Leu Trp Val Ser Met Val Glu Lys Ala Tyr Ala Lys 1825 1830 1835 1840 ctc cat ggt tct tat gag gca ctg gag ggg gga ctg gtt cag gat gct 5687 Leu His Gly Ser Tyr Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala 1845 1850 1855 ctt gtc gac cta act gga gga gct ggt gag gag att gac ttg cgg agt 5735 Leu Val Asp Leu Thr Gly Gly Ala Gly Glu Glu Ile Asp Leu Arg Ser 1860 1865 1870 gct caa gca caa ata gat ctt gca agt ggc aga ttg tgg tct caa ttg 5783 Ala Gln Ala Gln Ile Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu 1875 1880 1885 tta cgt ttt aaa caa gag ggg ttc tta ctt ggt gct gga agt cca tca 5831 Leu Arg Phe Lys Gln Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser 1890 1895 1900 gga tct gat gtt cat gta tct tcc agt ggc att gtg caa ggg

cat gct 5879 Gly Ser Asp Val His Val Ser Ser Ser Gly Ile Val Gln Gly His Ala 1905 1910 1915 1920 tac tcc gtc tta cag gtg aga gag gtt gat ggg cac aga ctt gtt cag 5927 Tyr Ser Val Leu Gln Val Arg Glu Val Asp Gly His Arg Leu Val Gln 1925 1930 1935 att cga aat cca tgg gct aat gaa gtt gag tgg aat ggt ccc tgg tca 5975 Ile Arg Asn Pro Trp Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser 1940 1945 1950 gac tca tcc cca gag tgg act gat agg atg aag cac aag ctg aag cat 6023 Asp Ser Ser Pro Glu Trp Thr Asp Arg Met Lys His Lys Leu Lys His 1955 1960 1965 gtt cca cag atg cgc tac tct gta aat ggc caa tgg cga ggt tat agt 6071 Val Pro Gln Met Arg Tyr Ser Val Asn Gly Gln Trp Arg Gly Tyr Ser 1970 1975 1980 gcc ggt ggc tgc caa gat tat agc tca tgg cat caa aat cca caa ttc 6119 Ala Gly Gly Cys Gln Asp Tyr Ser Ser Trp His Gln Asn Pro Gln Phe 1985 1990 1995 2000 agg ctg agg gca act ggt tct gat gca tct tta cca att cat ggc gta 6167 Arg Leu Arg Ala Thr Gly Ser Asp Ala Ser Leu Pro Ile His Gly Val 2005 2010 2015 ggt ttc tcg aga aca act cct gga ttt cgt aac tac caa tca agc cat 6215 Gly Phe Ser Arg Thr Thr Pro Gly Phe Arg Asn Tyr Gln Ser Ser His 2020 2025 2030 gat tca cag ttg ttc tat atc gga ttg agg att ctt aaa act cgt gga 6263 Asp Ser Gln Leu Phe Tyr Ile Gly Leu Arg Ile Leu Lys Thr Arg Gly 2035 2040 2045 cgt cgt gct gct tac aac ata ttt ctt cat gaa tct gtt ggt gga aca 6311 Arg Arg Ala Ala Tyr Asn Ile Phe Leu His Glu Ser Val Gly Gly Thr 2050 2055 2060 gac tat gtg aat tcc cgt gag att tca tgt gaa atg gtt ctt gac cct 6359 Asp Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Met Val Leu Asp Pro 2065 2070 2075 2080 gat cct aag ggt tat act att gtc cca acc acg ata cac cca ggg gaa 6407 Asp Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile His Pro Gly Glu 2085 2090 2095 gaa gca cct ttt gtc ctt tca gtc ttc aca aaa gca tcc att gtt ctt 6455 Glu Ala Pro Phe Val Leu Ser Val Phe Thr Lys Ala Ser Ile Val Leu 2100 2105 2110 gaa gct ttg t ag 6467 Glu Ala Leu 2115 4 2115 PRT arabidopsis 4 Met Glu Gly Asp Glu Arg Gly Val Leu Leu Ala Cys Val Ile Ser Gly 1 5 10 15 Thr Leu Phe Thr Val Phe Gly Ser Gly Ser Phe Trp Ile Leu Trp Ala 20 25 30 Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Phe Ala Arg Lys 35 40 45 Trp Pro Lys Val Leu Gln Gly Pro Gln Leu Asp Ile Leu Cys Gly Val 50 55 60 Leu Ser Leu Phe Ala Trp Ile Val Val Val Ser Pro Ile Ala Ile Leu 65 70 75 80 Ile Gly Trp Gly Ser Trp Leu Ile Val Ile Leu Asp Arg His Ile Ile 85 90 95 Gly Leu Ala Ile Ile Met Ala Gly Thr Ala Leu Leu Leu Ala Phe Tyr 100 105 110 Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Arg Ala Val 115 120 125 Ala Leu Leu Leu Leu Leu Gly Val Ala Leu Leu Cys Ala Tyr Glu Leu 130 135 140 Cys Ala Val Tyr Val Thr Ala Gly Ala His Ala Ser Gln Gln Tyr Ser 145 150 155 160 Pro Ser Gly Phe Phe Phe Gly Val Ser Ala Ile Ala Leu Ala Ile Asn 165 170 175 Met Leu Phe Ile Cys Arg Met Val Phe Asn Gly Asn Gly Leu Asp Val 180 185 190 Asp Glu Tyr Val Arg Arg Ala Tyr Lys Phe Ala Tyr Ser Asp Cys Ile 195 200 205 Glu Val Gly Pro Val Ala Cys Leu Pro Glu Pro Pro Asp Pro Asn Glu 210 215 220 Leu Tyr Pro Arg Gln Thr Ser Arg Ala Ser His Leu Gly Leu Leu Tyr 225 230 235 240 Leu Gly Ser Leu Val Val Leu Leu Ala Tyr Ser Val Leu Tyr Gly Leu 245 250 255 Thr Ala Arg Glu Ser Arg Trp Leu Gly Gly Ile Thr Ser Ala Ala Val 260 265 270 Ile Val Leu Asp Trp Asn Ile Gly Ala Cys Leu Tyr Gly Phe Lys Leu 275 280 285 Leu Gln Asn Arg Val Leu Ala Leu Phe Val Ala Gly Ile Ser Arg Leu 290 295 300 Phe Leu Ile Cys Phe Gly Ile His Tyr Trp Tyr Leu Gly His Cys Ile 305 310 315 320 Ser Tyr Ile Phe Val Ala Ser Val Leu Ser Gly Ala Ala Val Ser Arg 325 330 335 His Leu Ser Ile Thr Asp Pro Ser Ala Ala Arg Arg Asp Ala Leu Gln 340 345 350 Ser Thr Val Ile Arg Leu Arg Glu Gly Phe Arg Arg Lys Glu Gln Asn 355 360 365 Ser Ser Ser Ser Ser Ser Asp Gly Cys Gly Ser Ser Ile Lys Arg Ser 370 375 380 Ser Ser Ile Asp Ala Gly His Thr Gly Cys Thr Asn Glu Ala Asn Arg 385 390 395 400 Thr Ala Glu Ser Cys Thr Ala Asp Asn Leu Thr Arg Thr Gly Ser Ser 405 410 415 Gln Glu Gly Ile Asn Ser Asp Lys Ser Glu Glu Ser Gly Arg Pro Ser 420 425 430 Leu Gly Leu Arg Ser Ser Ser Cys Arg Ser Val Val Gln Glu Pro Glu 435 440 445 Ala Gly Thr Ser Tyr Phe Met Asp Lys Val Ser Asp Gln Asn Asn Thr 450 455 460 Leu Val Val Cys Ser Ser Ser Gly Leu Asp Ser Gln Gly Tyr Glu Ser 465 470 475 480 Ser Thr Ser Asn Ser Ala Asn Gln Gln Leu Leu Asp Met Asn Leu Ala 485 490 495 Leu Ala Phe Gln Asp Gln Leu Asn Asn Pro Arg Ile Ala Ser Ile Leu 500 505 510 Lys Lys Lys Ala Lys Glu Gly Asp Leu Glu Leu Thr Asn Leu Leu Gln 515 520 525 Asp Lys Gly Leu Asp Pro Asn Phe Ala Val Met Leu Lys Glu Lys Asn 530 535 540 Leu Asp Pro Thr Ile Leu Ala Leu Leu Gln Arg Ser Ser Leu Asp Ala 545 550 555 560 Asp Arg Asp His Arg Asp Asn Thr Asp Ile Thr Ile Ile Asp Ser Asn 565 570 575 Ser Val Asp Asn Thr Leu Pro Asn Gln Ile Ser Leu Ser Glu Glu Leu 580 585 590 Arg Leu Arg Gly Leu Glu Lys Trp Leu Lys Leu Ser Arg Leu Leu Leu 595 600 605 His His Val Ala Gly Thr Pro Glu Arg Ala Trp Gly Leu Phe Ser Leu 610 615 620 Val Phe Ile Leu Glu Thr Ile Ile Val Ala Ile Phe Arg Pro Lys Thr 625 630 635 640 Ile Thr Ile Ile Asn Ser Ser His Gln Gln Phe Glu Phe Gly Phe Ser 645 650 655 Val Leu Leu Leu Ser Pro Val Val Cys Ser Ile Met Ala Phe Leu Arg 660 665 670 Ser Leu Gln Val Glu Glu Met Ala Leu Thr Ser Lys Ser Arg Lys Tyr 675 680 685 Gly Phe Val Ala Trp Leu Leu Ser Thr Ser Val Gly Leu Ser Leu Ser 690 695 700 Phe Leu Ser Lys Ser Ser Val Leu Leu Gly Ile Ser Leu Thr Val Pro 705 710 715 720 Leu Met Ala Ala Cys Leu Ser Ile Ala Val Pro Ile Trp Met His Asn 725 730 735 Gly Tyr Gln Phe Trp Val Pro Gln Leu Ser Cys Gly Asp Gln Ala Arg 740 745 750 Asp Leu Arg Ser Pro Arg Ile Lys Gly Phe Ile Leu Trp Ile Cys Val 755 760 765 Val Leu Phe Ala Gly Ser Val Ile Ser Leu Gly Ala Ile Ile Ser Ala 770 775 780 Lys Pro Leu Asp Asp Leu Lys Tyr Lys Leu Phe Ser Ala Arg Glu Asn 785 790 795 800 Asn Val Thr Ser Pro Tyr Thr Ser Ser Val Tyr Leu Gly Trp Ala Met 805 810 815 Ser Ser Gly Ile Ala Leu Val Val Thr Ala Ile Leu Pro Ile Val Ser 820 825 830 Trp Phe Ala Thr Tyr Arg Phe Ser His Ser Ser Ala Val Cys Leu Met 835 840 845 Ile Phe Ser Val Val Leu Val Ala Phe Cys Gly Thr Ser Tyr Leu Glu 850 855 860 Val Val Lys Ser Arg Asp Asp Gln Leu Pro Thr Lys Gly Asp Phe Leu 865 870 875 880 Ala Ala Leu Leu Pro Leu Ala Cys Ile Pro Ala Leu Leu Ser Leu Cys 885 890 895 Cys Gly Met Val Lys Trp Lys Asp Asp Cys Trp Ile Leu Ser Arg Gly 900 905 910 Val Tyr Val Phe Phe Ser Ile Gly Leu Leu Leu Leu Phe Gly Ala Ile 915 920 925 Ala Ala Val Ile Ala Val Lys Pro Trp Thr Ile Gly Val Ser Phe Leu 930 935 940 Leu Val Leu Phe Leu Met Val Val Thr Ile Gly Val Ile His Leu Trp 945 950 955 960 Ala Ser Asn Asn Phe Tyr Leu Thr Arg Lys Gln Thr Ser Phe Val Cys 965 970 975 Phe Leu Ala Leu Leu Leu Gly Leu Ala Ala Phe Leu Leu Gly Trp His 980 985 990 Gln Asp Lys Ala Phe Ala Gly Ala Ser Val Gly Tyr Phe Thr Phe Leu 995 1000 1005 Ser Leu Leu Ala Gly Arg Ala Leu Ala Val Leu Leu Ser Pro Pro Ile 1010 1015 1020 Val Val Tyr Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His 1025 1030 1035 1040 Ala Asp Cys Gly Lys Asn Val Ser Ala Ala Phe Leu Val Leu Tyr Gly 1045 1050 1055 Ile Ala Leu Ala Thr Glu Gly Trp Gly Val Val Ala Ser Leu Ile Ile 1060 1065 1070 Tyr Pro Pro Phe Ala Gly Ala Ala Val Ser Ala Ile Thr Leu Val Val 1075 1080 1085 Ala Phe Gly Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Glu Met Met 1090 1095 1100 Glu Val Ala Val Arg Phe Leu Ser Lys Asp Thr Ile Val Gln Ala Ile 1105 1110 1115 1120 Ser Arg Ser Ala Thr Lys Thr Arg Asn Ala Leu Ser Gly Thr Tyr Ser 1125 1130 1135 Ala Pro Gln Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro 1140 1145 1150 Ser Ala Met Arg Asp Lys Ala Gly Asn Phe Val Leu Pro Arg Asp Asp 1155 1160 1165 Val Met Lys Leu Arg Asp Arg Leu Arg Asn Glu Glu Arg Val Ala Gly 1170 1175 1180 Ser Ile Phe Tyr Lys Met Gln Cys Arg Lys Gly Phe Arg His Glu Pro 1185 1190 1195 1200 Pro Thr Asn Val Asp Tyr Arg Arg Asp Met Cys Ala His Ala Arg Val 1205 1210 1215 Leu Ala Leu Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp 1220 1225 1230 Lys Phe Gly Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala 1235 1240 1245 Glu Arg Val Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile 1250 1255 1260 Gly Phe Ser Asp Leu Ser Ala Arg Lys Ile Ser Lys Trp Lys Pro Glu 1265 1270 1275 1280 Asp Arg Arg Gln Phe Glu Ile Ile Gln Glu Ser Tyr Leu Arg Glu Lys 1285 1290 1295 Glu Met Glu Glu Glu Ser Leu Met Gln Arg Arg Glu Glu Glu Gly Arg 1300 1305 1310 Gly Lys Glu Arg Arg Lys Ala Leu Leu Glu Lys Glu Glu Arg Lys Trp 1315 1320 1325 Lys Glu Ile Glu Ala Ser Leu Ile Pro Ser Ile Pro Asn Ala Gly Ser 1330 1335 1340 Arg Glu Ala Ala Ala Met Ala Ala Ala Ile Arg Ala Val Gly Gly Asp 1345 1350 1355 1360 Ser Val Leu Glu Asp Ser Phe Ala Arg Glu Arg Val Ser Gly Ile Ala 1365 1370 1375 Arg Arg Ile Arg Thr Ala Gln Leu Glu Arg Arg Ala Gln Gln Thr Gly 1380 1385 1390 Ile Ser Gly Ala Val Cys Val Leu Asp Asp Glu Pro Met Ile Ser Gly 1395 1400 1405 Lys His Cys Gly Gln Met Asp Ser Ser Val Cys Gln Ser Gln Lys Ile 1410 1415 1420 Ser Phe Ser Val Thr Ala Met Ile Gln Ser Asp Ser Gly Pro Val Cys 1425 1430 1435 1440 Leu Phe Gly Thr Glu Phe Gln Lys Lys Val Cys Trp Glu Ile Leu Val 1445 1450 1455 Ala Gly Ser Glu Gln Gly Ile Glu Ala Gly Gln Val Gly Leu Arg Leu 1460 1465 1470 Ile Thr Lys Gly Glu Arg Gln Thr Thr Val Ala Arg Glu Trp Tyr Ile 1475 1480 1485 Gly Ala Thr Ser Ile Thr Asp Gly Arg Trp His Thr Val Thr Ile Thr 1490 1495 1500 Ile Asp Ala Asp Ala Gly Glu Ala Thr Cys Tyr Ile Asp Gly Gly Phe 1505 1510 1515 1520 Asp Gly Tyr Gln Asn Gly Leu Pro Leu Ser Ile Gly Ser Ala Ile Trp 1525 1530 1535 Glu Gln Gly Ala Glu Val Trp Leu Gly Val Arg Pro Pro Ile Asp Val 1540 1545 1550 Asp Ala Phe Gly Arg Ser Asp Ser Asp Gly Val Glu Ser Lys Met His 1555 1560 1565 Ile Met Asp Val Phe Leu Trp Gly Lys Cys Leu Ser Glu Glu Glu Ala 1570 1575 1580 Ala Ser Leu His Ala Ala Ile Gly Met Ala Asp Leu Asp Met Ile Asp 1585 1590 1595 1600 Leu Ser Asp Asp Asn Trp Gln Trp Thr Asp Ser Pro Pro Arg Val Asp 1605 1610 1615 Gly Trp Asp Ser Asp Pro Ala Asp Val Asp Leu Tyr Asp Arg Asp Asp 1620 1625 1630 Val Asp Trp Asp Gly Gln Tyr Ser Ser Gly Arg Lys Arg Arg Ser Gly 1635 1640 1645 Arg Asp Phe Val Met Ser Val Asp Ser Phe Ala Arg Arg His Arg Lys 1650 1655 1660 Pro Arg Met Glu Thr Gln Glu Asp Ile Asn Gln Arg Met Arg Ser Val 1665 1670 1675 1680 Glu Leu Ala Val Lys Glu Ala Leu Ser Ala Arg Gly Asp Lys Gln Phe 1685 1690 1695 Thr Asp Gln Glu Phe Pro Pro Asn Asp Arg Ser Leu Phe Val Asp Thr 1700 1705 1710 Gln Asn Pro Pro Ser Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro 1715 1720 1725 Asp Ser Ile Val Lys Glu Asn Gly Ser Asp Ser Arg Pro Cys Leu Phe 1730 1735 1740 Ser Gly Asp Ala Asn Pro Ser Asp Val Cys Gln Gly Arg Leu Gly Asp 1745 1750 1755 1760 Cys Trp Phe Leu Ser Ala Val Ala Val Leu Thr Glu Val Ser Arg Ile 1765 1770 1775 Ser Glu Val Ile Ile Thr Pro Glu Tyr Asn Glu Glu Gly Ile Tyr Thr 1780 1785 1790 Val Arg Phe Cys Ile Gln Gly Glu Trp Val Pro Val Val Ile Asp Asp 1795 1800 1805 Trp Ile Pro Cys Glu Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg 1810 1815 1820 Lys Leu Asn Glu Leu Trp Val Ser Met Val Glu Lys Ala Tyr Ala Lys 1825 1830 1835 1840 Leu His Gly Ser Tyr Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala 1845 1850 1855 Leu Val Asp Leu Thr Gly Gly Ala Gly Glu Glu Ile Asp Leu Arg Ser 1860 1865 1870 Ala Gln Ala Gln Ile Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu 1875 1880 1885 Leu Arg Phe Lys Gln Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser 1890 1895 1900 Gly Ser Asp Val His Val Ser Ser Ser Gly Ile Val Gln Gly His Ala 1905 1910 1915 1920 Tyr Ser Val Leu Gln Val Arg Glu Val Asp Gly His Arg Leu Val Gln 1925 1930 1935 Ile Arg Asn Pro Trp Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser 1940 1945 1950 Asp Ser Ser Pro Glu Trp Thr Asp Arg Met Lys His Lys Leu Lys His 1955 1960 1965 Val Pro Gln Met Arg Tyr Ser Val Asn Gly Gln Trp Arg Gly Tyr Ser 1970 1975 1980 Ala Gly Gly Cys Gln Asp Tyr Ser Ser Trp His Gln Asn Pro Gln Phe 1985 1990 1995 2000 Arg Leu Arg Ala Thr Gly Ser Asp Ala Ser Leu Pro Ile His Gly Val 2005 2010 2015 Gly Phe Ser Arg Thr Thr Pro Gly Phe Arg Asn Tyr Gln Ser Ser His 2020 2025 2030 Asp Ser Gln Leu Phe Tyr Ile Gly Leu Arg Ile Leu Lys Thr Arg Gly 2035 2040 2045 Arg Arg Ala Ala Tyr Asn Ile Phe Leu His Glu Ser Val Gly Gly Thr 2050 2055 2060 Asp Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Met Val Leu Asp Pro 2065 2070 2075 2080 Asp Pro Lys Gly Tyr Thr

Ile Val Pro Thr Thr Ile His Pro Gly Glu 2085 2090 2095 Glu Ala Pro Phe Val Leu Ser Val Phe Thr Lys Ala Ser Ile Val Leu 2100 2105 2110 Glu Ala Leu 2115 5 1007 DNA zea mays CDS (150)...(762) maize superal 5 gtcgtcaaaa aatccccatt cgttcccctt ctcgcctcca cgagccttca caaattgccc 60 tctcagctct tctcctaatc tcactctagc cctagccaaa tcccagaccc atcccagccc 120 tagcgacacc aaccagctcg ccagcgacc atg ggg aac ccg gag aag ctg atg 173 Met Gly Asn Pro Glu Lys Leu Met 1 5 aat cag atc ttc gac ctc aag ttc acc tcc aag tcg ctg cag cgg cag 221 Asn Gln Ile Phe Asp Leu Lys Phe Thr Ser Lys Ser Leu Gln Arg Gln 10 15 20 gcg cgc aag tgc gag aag gag gag aag gag cag aag ctc aag gtc aag 269 Ala Arg Lys Cys Glu Lys Glu Glu Lys Glu Gln Lys Leu Lys Val Lys 25 30 35 40 aag gcg atc gag aag ggc aac atg gac ggc gcc cgc atc tac gcc gag 317 Lys Ala Ile Glu Lys Gly Asn Met Asp Gly Ala Arg Ile Tyr Ala Glu 45 50 55 aac gcc atc cgc aag cgc acc gag cac atg aac tac ctc cgc ctc gcc 365 Asn Ala Ile Arg Lys Arg Thr Glu His Met Asn Tyr Leu Arg Leu Ala 60 65 70 tct cgc ctc gac gcc gtc gtg gcc cgc ctc gac acg cag gcc aag atg 413 Ser Arg Leu Asp Ala Val Val Ala Arg Leu Asp Thr Gln Ala Lys Met 75 80 85 cag gtc atc ggc aag tcc atg cag tcc atc gtc aag tcg ctc gac tcc 461 Gln Val Ile Gly Lys Ser Met Gln Ser Ile Val Lys Ser Leu Asp Ser 90 95 100 tcg ctc gcc acc ggg aac ctc cag aag atg tcc gag acc atg gac aat 509 Ser Leu Ala Thr Gly Asn Leu Gln Lys Met Ser Glu Thr Met Asp Asn 105 110 115 120 ttc gag cgc cag ttc gtc aac atg gag gtc cag gcc gag ttc atg gag 557 Phe Glu Arg Gln Phe Val Asn Met Glu Val Gln Ala Glu Phe Met Glu 125 130 135 ggc gcc atg gcc ggc tcc acc tcc ctc tcc acg ccg gag acc gag gtc 605 Gly Ala Met Ala Gly Ser Thr Ser Leu Ser Thr Pro Glu Thr Glu Val 140 145 150 aac agc ctc atg cag cag gtc gcg gac gac tac ggg ctc gag gtc tcc 653 Asn Ser Leu Met Gln Gln Val Ala Asp Asp Tyr Gly Leu Glu Val Ser 155 160 165 gtc ggt ctg ccg cag gca gcc gcc cac gcc atc cct gcc gcc aag gat 701 Val Gly Leu Pro Gln Ala Ala Ala His Ala Ile Pro Ala Ala Lys Asp 170 175 180 aag gag aaa gtc gac gag gat gac ctt tct cgc cgc ctc gcc gag ctt 749 Lys Glu Lys Val Asp Glu Asp Asp Leu Ser Arg Arg Leu Ala Glu Leu 185 190 195 200 aag gcc cgc ggc t gagagggtgt ttcaggcttt atcagatgcg attcaggctt 802 Lys Ala Arg Gly atgctgttga gccgcaatga cccattgtcc tgtggactgc tactcatgtt tggtagattc 862 gtaaatacta ctgttatatg cttgaaggcg tgtgttggca ttgccatgtg gggtttgtgt 922 gactcgatgg aaactttgct gtcttattgt actttaaaat ccaataattt gcgcgattgc 982 ggacaaaaaa aaaaacaaaa aaaaa 1007 6 204 PRT zea mays 6 Met Gly Asn Pro Glu Lys Leu Met Asn Gln Ile Phe Asp Leu Lys Phe 1 5 10 15 Thr Ser Lys Ser Leu Gln Arg Gln Ala Arg Lys Cys Glu Lys Glu Glu 20 25 30 Lys Glu Gln Lys Leu Lys Val Lys Lys Ala Ile Glu Lys Gly Asn Met 35 40 45 Asp Gly Ala Arg Ile Tyr Ala Glu Asn Ala Ile Arg Lys Arg Thr Glu 50 55 60 His Met Asn Tyr Leu Arg Leu Ala Ser Arg Leu Asp Ala Val Val Ala 65 70 75 80 Arg Leu Asp Thr Gln Ala Lys Met Gln Val Ile Gly Lys Ser Met Gln 85 90 95 Ser Ile Val Lys Ser Leu Asp Ser Ser Leu Ala Thr Gly Asn Leu Gln 100 105 110 Lys Met Ser Glu Thr Met Asp Asn Phe Glu Arg Gln Phe Val Asn Met 115 120 125 Glu Val Gln Ala Glu Phe Met Glu Gly Ala Met Ala Gly Ser Thr Ser 130 135 140 Leu Ser Thr Pro Glu Thr Glu Val Asn Ser Leu Met Gln Gln Val Ala 145 150 155 160 Asp Asp Tyr Gly Leu Glu Val Ser Val Gly Leu Pro Gln Ala Ala Ala 165 170 175 His Ala Ile Pro Ala Ala Lys Asp Lys Glu Lys Val Asp Glu Asp Asp 180 185 190 Leu Ser Arg Arg Leu Ala Glu Leu Lys Ala Arg Gly 195 200 7 612 DNA arabidopsis CDS (1)...(612) arabidopsis superal homolog 1 7 atg ggt aac aca gat aag ctg atg aat cag ata ttc gat ttg aaa ttt 48 Met Gly Asn Thr Asp Lys Leu Met Asn Gln Ile Phe Asp Leu Lys Phe 1 5 10 15 acg tca aag tct ctg caa agg caa tca agg aag tgt gag aag gaa gag 96 Thr Ser Lys Ser Leu Gln Arg Gln Ser Arg Lys Cys Glu Lys Glu Glu 20 25 30 aaa gca gag aaa ttg aag gtg aag aag gct atc gag aag ggt aat atg 144 Lys Ala Glu Lys Leu Lys Val Lys Lys Ala Ile Glu Lys Gly Asn Met 35 40 45 gat ggt gct cgg atc tac gct gag aac gcc att cgt aag cgt agc gag 192 Asp Gly Ala Arg Ile Tyr Ala Glu Asn Ala Ile Arg Lys Arg Ser Glu 50 55 60 cag atg aac tat ctc cgt ctc gct tct cgc ctt gac gct gtt gtt gct 240 Gln Met Asn Tyr Leu Arg Leu Ala Ser Arg Leu Asp Ala Val Val Ala 65 70 75 80 cgt ctt gat act cag gct aag atg acc acc atc acc aaa tcc atg acc 288 Arg Leu Asp Thr Gln Ala Lys Met Thr Thr Ile Thr Lys Ser Met Thr 85 90 95 aat atc gtc aaa tcc ctt gag tct tct ctt gcc aca ggg aat cta cag 336 Asn Ile Val Lys Ser Leu Glu Ser Ser Leu Ala Thr Gly Asn Leu Gln 100 105 110 aag atg tca gag aca atg gat tca ttc gag aag cag ttt gtg aac atg 384 Lys Met Ser Glu Thr Met Asp Ser Phe Glu Lys Gln Phe Val Asn Met 115 120 125 gag gtc caa gct gag ttc atg gag aat gct atg gct ggt tca act tca 432 Glu Val Gln Ala Glu Phe Met Glu Asn Ala Met Ala Gly Ser Thr Ser 130 135 140 ttg tcc act cca gaa ggc gaa gtc aac agc ctt atg cag cag gtg gca 480 Leu Ser Thr Pro Glu Gly Glu Val Asn Ser Leu Met Gln Gln Val Ala 145 150 155 160 gat gac tat ggt ttg gaa gtc tct gtg ggg ctt cct cag cct gct ggt 528 Asp Asp Tyr Gly Leu Glu Val Ser Val Gly Leu Pro Gln Pro Ala Gly 165 170 175 cat gcc att cct act aag act gag gag aaa gtc gat gag gat gat ttg 576 His Ala Ile Pro Thr Lys Thr Glu Glu Lys Val Asp Glu Asp Asp Leu 180 185 190 tcg agg agg ctt gcg gag ctt aaa gcc aga gga taa 612 Ser Arg Arg Leu Ala Glu Leu Lys Ala Arg Gly * 195 200 8 203 PRT arabidopsis 8 Met Gly Asn Thr Asp Lys Leu Met Asn Gln Ile Phe Asp Leu Lys Phe 1 5 10 15 Thr Ser Lys Ser Leu Gln Arg Gln Ser Arg Lys Cys Glu Lys Glu Glu 20 25 30 Lys Ala Glu Lys Leu Lys Val Lys Lys Ala Ile Glu Lys Gly Asn Met 35 40 45 Asp Gly Ala Arg Ile Tyr Ala Glu Asn Ala Ile Arg Lys Arg Ser Glu 50 55 60 Gln Met Asn Tyr Leu Arg Leu Ala Ser Arg Leu Asp Ala Val Val Ala 65 70 75 80 Arg Leu Asp Thr Gln Ala Lys Met Thr Thr Ile Thr Lys Ser Met Thr 85 90 95 Asn Ile Val Lys Ser Leu Glu Ser Ser Leu Ala Thr Gly Asn Leu Gln 100 105 110 Lys Met Ser Glu Thr Met Asp Ser Phe Glu Lys Gln Phe Val Asn Met 115 120 125 Glu Val Gln Ala Glu Phe Met Glu Asn Ala Met Ala Gly Ser Thr Ser 130 135 140 Leu Ser Thr Pro Glu Gly Glu Val Asn Ser Leu Met Gln Gln Val Ala 145 150 155 160 Asp Asp Tyr Gly Leu Glu Val Ser Val Gly Leu Pro Gln Pro Ala Gly 165 170 175 His Ala Ile Pro Thr Lys Thr Glu Glu Lys Val Asp Glu Asp Asp Leu 180 185 190 Ser Arg Arg Leu Ala Glu Leu Lys Ala Arg Gly 195 200 9 669 DNA arabidopsis CDS (1)...(669) arabidopsis superal homolog 2 9 atg ggt aat aca gat aag ctg atg aac cag atc ttt gaa ttg aaa ttt 48 Met Gly Asn Thr Asp Lys Leu Met Asn Gln Ile Phe Glu Leu Lys Phe 1 5 10 15 acg tca aag tct ctg cag agg caa gct agg aag tgc gaa aaa gag gag 96 Thr Ser Lys Ser Leu Gln Arg Gln Ala Arg Lys Cys Glu Lys Glu Glu 20 25 30 aga tcg gag aag ctc aag gta aag aaa gcc att gag aaa ggt aac atg 144 Arg Ser Glu Lys Leu Lys Val Lys Lys Ala Ile Glu Lys Gly Asn Met 35 40 45 gat ggt gct cgg atc tat gcc gag aac gcc att cgc aaa cgc agc gag 192 Asp Gly Ala Arg Ile Tyr Ala Glu Asn Ala Ile Arg Lys Arg Ser Glu 50 55 60 cag atg aac tac ctt cgt ctc tct tct cga ttg gac gct gtt gtt gct 240 Gln Met Asn Tyr Leu Arg Leu Ser Ser Arg Leu Asp Ala Val Val Ala 65 70 75 80 cga ctc gat acc cag gct aag atg gct acc atc acc aaa tcg atg acc 288 Arg Leu Asp Thr Gln Ala Lys Met Ala Thr Ile Thr Lys Ser Met Thr 85 90 95 aac att gtc aaa tcc ctc gaa tcg tct ctt acc act ggc aac tta cag 336 Asn Ile Val Lys Ser Leu Glu Ser Ser Leu Thr Thr Gly Asn Leu Gln 100 105 110 aag atg tct gag acg atg gat tcg ttt gag aaa cag ttt gtg aac atg 384 Lys Met Ser Glu Thr Met Asp Ser Phe Glu Lys Gln Phe Val Asn Met 115 120 125 gaa gtc caa gct gag ttc atg gat aat gca atg gct ggc tct act tca 432 Glu Val Gln Ala Glu Phe Met Asp Asn Ala Met Ala Gly Ser Thr Ser 130 135 140 ttg tcg act cca gaa gga gaa gtc aac agc ttg atg cag caa gta gca 480 Leu Ser Thr Pro Glu Gly Glu Val Asn Ser Leu Met Gln Gln Val Ala 145 150 155 160 gat gat tac ggt ctt gaa gtt tct gtt gga cta cct cag cct gct ggt 528 Asp Asp Tyr Gly Leu Glu Val Ser Val Gly Leu Pro Gln Pro Ala Gly 165 170 175 cat gcc att cct acc aag act gaa gag aaa gtt gag gag gat gat tta 576 His Ala Ile Pro Thr Lys Thr Glu Glu Lys Val Glu Glu Asp Asp Leu 180 185 190 acc agg aga ctt gcc gag ctt aaa gcc aga gat gtg tct ctg gta gta 624 Thr Arg Arg Leu Ala Glu Leu Lys Ala Arg Asp Val Ser Leu Val Val 195 200 205 gtt cca cca aat ttt cca ggt cca gaa agg aat aca ctg gag taa 669 Val Pro Pro Asn Phe Pro Gly Pro Glu Arg Asn Thr Leu Glu * 210 215 220 10 222 PRT arabidopsis 10 Met Gly Asn Thr Asp Lys Leu Met Asn Gln Ile Phe Glu Leu Lys Phe 1 5 10 15 Thr Ser Lys Ser Leu Gln Arg Gln Ala Arg Lys Cys Glu Lys Glu Glu 20 25 30 Arg Ser Glu Lys Leu Lys Val Lys Lys Ala Ile Glu Lys Gly Asn Met 35 40 45 Asp Gly Ala Arg Ile Tyr Ala Glu Asn Ala Ile Arg Lys Arg Ser Glu 50 55 60 Gln Met Asn Tyr Leu Arg Leu Ser Ser Arg Leu Asp Ala Val Val Ala 65 70 75 80 Arg Leu Asp Thr Gln Ala Lys Met Ala Thr Ile Thr Lys Ser Met Thr 85 90 95 Asn Ile Val Lys Ser Leu Glu Ser Ser Leu Thr Thr Gly Asn Leu Gln 100 105 110 Lys Met Ser Glu Thr Met Asp Ser Phe Glu Lys Gln Phe Val Asn Met 115 120 125 Glu Val Gln Ala Glu Phe Met Asp Asn Ala Met Ala Gly Ser Thr Ser 130 135 140 Leu Ser Thr Pro Glu Gly Glu Val Asn Ser Leu Met Gln Gln Val Ala 145 150 155 160 Asp Asp Tyr Gly Leu Glu Val Ser Val Gly Leu Pro Gln Pro Ala Gly 165 170 175 His Ala Ile Pro Thr Lys Thr Glu Glu Lys Val Glu Glu Asp Asp Leu 180 185 190 Thr Arg Arg Leu Ala Glu Leu Lys Ala Arg Asp Val Ser Leu Val Val 195 200 205 Val Pro Pro Asn Phe Pro Gly Pro Glu Arg Asn Thr Leu Glu 210 215 220 11 607 DNA Oryza sativa allele (1)...(607) Rice superal homolog misc_feature (1)...(607) n = A,T,C or G 11 tgaattcggc acgaggcnna atcccccaat ccgatctgcc tttcctcttc ctcctcgtca 60 aaccctagcc aaatccacga tcggggctag ctcgtcggcg gcggcgatgg ggaacccgga 120 gaagctgatg acccagatct tcgacctcaa gttcacctcc aagtcgctgc agcggcaggc 180 gcgcaagtgc gagaaggagg agaaggagca gaagctcaag gtgaagaagg cgatcgagaa 240 ggggaacatg gacggcgcgc ggatctacgc cgagaacgcc atccgcaagc gcaccgagca 300 catgaactac ctccgcctcg cctcccgcct cgacgccgtc gtcgcgcgcc tcgacaccca 360 ggcgaagatg caggtcatcg gcaagtccat ggccaacatc gtcaagtcgc tcgactccgc 420 gctcgccacg gggaacctgc agaagatgtc cgagaccatg gacaacttcg agcgccagtt 480 cgtcaacatg gaggtccagg ccgagttcat ggagggcgcc atggccggct ccacctcgct 540 ctccacgccc gagaccgnnn tcaacagcct catgcagcaa gtcgccgacg actacggcct 600 tgaggtc 607 12 529 DNA Triticum aestivum allele (1)...(529) Wheat superal homolog 12 gcacgaggcc caatccccga tctcccgacc ccgacccccg acgaaaccct agccagcaaa 60 ccgcggcggc ggcggcaatg ggcaacccgg agaagctgat ggcgcagatc ttcgacctca 120 agttcacgtc caagtcgctg cagcggcagg cgcgcaagtg cgagaaggag gagaaggagc 180 agaagctcaa ggtgaagaag gccatcgaga agggcaacgt cgacggcgcg cggatctacg 240 ccgagaacgc catccgcaag cgcaccgagc acatgaacta cctccgcctc gcctcccgcc 300 tcgacgccgt cgtcgcgcgc ctcgacacgc aggccaagat gcaggccatc ggcaagtcca 360 tgggcagcat cgtcaagtcg cttgactcct ccctcgccac gggcaacctc cagaagatgt 420 ccgagaccat ggacagcttc gagcgccagt tcgtcaacat ggaggtccag gccgagttca 480 tggagggcgc catggccggc tccacctccc tctccacgcc cgagaccga 529 13 469 DNA Glycine max allele (1)...(469) Soybean superal homolog 13 ggaacacgga gaagctgatg aaccagatca tggaattgaa gttcacgtcg aaatcgctgc 60 agcggcaatc gcgtaagtgc gagaaggagg agaaatcgga gaagctgaag gtgaagaagg 120 cgatcgagaa aggcaacatg gacggagcgc ggatctacgc ggagaacgcg atccgtaagc 180 gcacggagca gatgaactac ctccggcttg cctcgcgcct ggacgccgtc gtggcccgcc 240 tcgacacgca ggcgaagatg acgacgatca gcaagtcgat gggcaacatc gtcaaatcgc 300 tggagtcgtc gctggccacg gggaacctcc agaagatgtc ggagaccatg gactcgttcg 360 agaagcagtt cgtgaacatg gaggtccagg ccgagttcat ggagagcgcc atggcgggat 420 ccacctccct ctccactccc gaaggagagg tcaacagcct catgcagca 469 14 484 DNA Hordeum vulgare allele (1)...(484) Barley superal homolog 14 gcacgagggt cgccctccct cccctaaaaa atccccaatc ctcctctcgc ccccctcccc 60 aatcccgacc cccgacgaaa ccctagctaa caaccggcgg cggcggcgat gggcaacccg 120 gagaagctga tggcgcagat cttcgacctc aagttcacct ccaagtcgct gcagcggcag 180 gcgcgcaagt gcgagaagga ggagaaggac cagaagctca aggtcaagaa ggccatcgag 240 aagggcaacg tcgacggcgc gcggatctac gccgagaacg ccatccgcaa gcgcaccgag 300 cacatgaact acctccgcct cgcctcccgc ctcgacgccg tcgtctcccg cctcgacacg 360 caggccaaga tgcaggccat cggaaagtcc atgggcaaca tcgtcaagtc gctcgactcc 420 tccctcgcca ccggcaacct ccagaagatg tccgagacca tggacaactt cgagcgccag 480 ttcg 484 15 3346 DNA Zea mays CDS (157)...(2862) maize cr4 15 caggggcctg gtgaaatgct gcgcagcgag ggtgactgac tggccactcc gcccgccgac 60 ccccgtttcg tgcttactgg catgctcgca gcataaagga atcttctcgg aattagcccc 120 ccttcctgtg gtcgtgacgg ccccaagttt caagac atg gac cat gtg ccg gct 174 Met Asp His Val Pro Ala 1 5 cta gtt ctt gcc ggg tgc tgc ttc ctg gcc ctg ctg cct ggc tgg gcc 222 Leu Val Leu Ala Gly Cys Cys Phe Leu Ala Leu Leu Pro Gly Trp Ala 10 15 20 tgt ggc ctt ggc tcc atg tcg tcc att gcg gtg tcc tat ggg gag gat 270 Cys Gly Leu Gly Ser Met Ser Ser Ile Ala Val Ser Tyr Gly Glu Asp 25 30 35 ggt ccg gtg ttc tgt ggc ctc aac tcc gac ggc tcc cac ctc gtc gcc 318 Gly Pro Val Phe Cys Gly Leu Asn Ser Asp Gly Ser His Leu Val Ala 40 45 50 tgc ttc ggt gct gat gca tct gtc ctg tac ggc gca cca ccc aac atc 366 Cys Phe Gly Ala Asp Ala Ser Val Leu Tyr Gly Ala Pro Pro Asn Ile 55 60 65 70 cct ttc ctt ggc ctc acg gca ggg gat ggg ttc gtg tgt ggc ctc ctg 414 Pro Phe Leu Gly Leu Thr Ala Gly Asp Gly Phe Val Cys Gly Leu Leu 75 80 85 ctc gac acc agg cag cca tac tgc tgg ggt agc aac tcc tat gtc aag 462 Leu Asp Thr Arg Gln Pro Tyr Cys Trp Gly Ser Asn Ser Tyr Val Lys 90 95 100 agc ggg gtg cca cag ccg atg gtt gag ggc gca agg tac tct gag ctc 510 Ser Gly Val Pro Gln Pro Met Val Glu Gly Ala Arg Tyr Ser Glu Leu 105 110 115 agt gcg ggg gac aac cac ctc tgc gca ctg aga gct gct caa gat ggg 558 Ser Ala Gly Asp Asn His Leu Cys Ala Leu Arg Ala Ala Gln Asp Gly 120

125 130 ggt cgt ggt tcc agt gct gct aca tcg ctg att gac tgc tgg gga tac 606 Gly Arg Gly Ser Ser Ala Ala Thr Ser Leu Ile Asp Cys Trp Gly Tyr 135 140 145 150 aat atg acc gcc aca cat gct gtt gat gaa gcc gtg tcg act gtt tca 654 Asn Met Thr Ala Thr His Ala Val Asp Glu Ala Val Ser Thr Val Ser 155 160 165 gct ggt tcg gtg ttc aat tgt ggc ttg ttt gct cgg aac agg acg gtg 702 Ala Gly Ser Val Phe Asn Cys Gly Leu Phe Ala Arg Asn Arg Thr Val 170 175 180 ttc tgc tgg ggc gac gag acg gtg agt ggt gtc gtt ggg ctg gca ccg 750 Phe Cys Trp Gly Asp Glu Thr Val Ser Gly Val Val Gly Leu Ala Pro 185 190 195 agg gat ctg cac ttt cag tct ata ggc gcg ggc ggt tac cat gtc tgt 798 Arg Asp Leu His Phe Gln Ser Ile Gly Ala Gly Gly Tyr His Val Cys 200 205 210 ggg gtg ttg gag aat gca cag gtg ttc tgc tgg ggc agg agc ttg gag 846 Gly Val Leu Glu Asn Ala Gln Val Phe Cys Trp Gly Arg Ser Leu Glu 215 220 225 230 atg cag cag gtg gtg cca tcc agt gct atc ggt gat ggt gat gtg aac 894 Met Gln Gln Val Val Pro Ser Ser Ala Ile Gly Asp Gly Asp Val Asn 235 240 245 ata gtg ccg atg gat gca atg agc act gtg gtt ggc ggg cgg ttc cat 942 Ile Val Pro Met Asp Ala Met Ser Thr Val Val Gly Gly Arg Phe His 250 255 260 gct tgt ggc atc agg agc ctt gac cac caa gtg gct tgc tgg ggc ttc 990 Ala Cys Gly Ile Arg Ser Leu Asp His Gln Val Ala Cys Trp Gly Phe 265 270 275 act ctt cat aac agt aca tcg cca cca aaa ggg ctg aag atg tat gct 1038 Thr Leu His Asn Ser Thr Ser Pro Pro Lys Gly Leu Lys Met Tyr Ala 280 285 290 ctt gtg gct ggg gat tac ttc act tgt gga gtg cct gct gag act tcg 1086 Leu Val Ala Gly Asp Tyr Phe Thr Cys Gly Val Pro Ala Glu Thr Ser 295 300 305 310 ctg atg ccg agg tgc tgg ggc aac agt ggg cca ttg gca tta ccc atg 1134 Leu Met Pro Arg Cys Trp Gly Asn Ser Gly Pro Leu Ala Leu Pro Met 315 320 325 gcc gta cct cct ggg att tgt gta cct act gca tgc agc cat ggg tac 1182 Ala Val Pro Pro Gly Ile Cys Val Pro Thr Ala Cys Ser His Gly Tyr 330 335 340 tat gaa tat gtg aac cat ggt gaa gtt ggc agc atc aag gtg tgt aag 1230 Tyr Glu Tyr Val Asn His Gly Glu Val Gly Ser Ile Lys Val Cys Lys 345 350 355 cct gca aac tct aga ctc tgc ttg ccc tgt agt aca ggt tgc ccg gaa 1278 Pro Ala Asn Ser Arg Leu Cys Leu Pro Cys Ser Thr Gly Cys Pro Glu 360 365 370 ggc ttg tat gag tca tct cct tgc aat gca aca gct gac cgt gtt tgc 1326 Gly Leu Tyr Glu Ser Ser Pro Cys Asn Ala Thr Ala Asp Arg Val Cys 375 380 385 390 cag ttt gat tgc ttg aag tgt gtc aca gat gag tgc ctg tca ttc tgc 1374 Gln Phe Asp Cys Leu Lys Cys Val Thr Asp Glu Cys Leu Ser Phe Cys 395 400 405 tta tca cag aag cgg acc aag agc cgc aag ttg atg gct ttt cag atg 1422 Leu Ser Gln Lys Arg Thr Lys Ser Arg Lys Leu Met Ala Phe Gln Met 410 415 420 cgc atc ttt gtt gca gag att gtc ttt gct gtc gtc ttg gta ctc agc 1470 Arg Ile Phe Val Ala Glu Ile Val Phe Ala Val Val Leu Val Leu Ser 425 430 435 gtg tca gta acc act tgc ctg tat gtc cgg cac aag ctt cga cat tgc 1518 Val Ser Val Thr Thr Cys Leu Tyr Val Arg His Lys Leu Arg His Cys 440 445 450 caa tgc tca aat aga gag ctg aga ctg gct aag agc aca gca tac tct 1566 Gln Cys Ser Asn Arg Glu Leu Arg Leu Ala Lys Ser Thr Ala Tyr Ser 455 460 465 470 ttc cgg aag gat aac atg aag atc cag cct gat atg gag gac ttg aag 1614 Phe Arg Lys Asp Asn Met Lys Ile Gln Pro Asp Met Glu Asp Leu Lys 475 480 485 atc agg aga gct cag gaa ttc tcc tat gaa gag tta gag caa gca acc 1662 Ile Arg Arg Ala Gln Glu Phe Ser Tyr Glu Glu Leu Glu Gln Ala Thr 490 495 500 ggt ggc ttc tca gag gat tca caa gtc ggc aaa ggc agc ttc tca tgt 1710 Gly Gly Phe Ser Glu Asp Ser Gln Val Gly Lys Gly Ser Phe Ser Cys 505 510 515 gta ttc aag ggc ata ctg aga gat ggg aca gtg gtt gct gtg aag cgt 1758 Val Phe Lys Gly Ile Leu Arg Asp Gly Thr Val Val Ala Val Lys Arg 520 525 530 gca ata aaa gca tca gat gtg aag aag agc tca aag gag ttt cac aac 1806 Ala Ile Lys Ala Ser Asp Val Lys Lys Ser Ser Lys Glu Phe His Asn 535 540 545 550 gaa ctt gac ctc cta tcc agg ctc aac cat gca cat ttg ctg aat ttg 1854 Glu Leu Asp Leu Leu Ser Arg Leu Asn His Ala His Leu Leu Asn Leu 555 560 565 ctt ggt tac tgc gag gat ggc agt gag agg ctc ttg gtt tat gag ttc 1902 Leu Gly Tyr Cys Glu Asp Gly Ser Glu Arg Leu Leu Val Tyr Glu Phe 570 575 580 atg gct cat gga tcc ctg tac cag cat ctg cat ggc aag gat cca aac 1950 Met Ala His Gly Ser Leu Tyr Gln His Leu His Gly Lys Asp Pro Asn 585 590 595 ttg aaa aag cga cta aac tgg gca agg cgg gtc acc att gct gta cag 1998 Leu Lys Lys Arg Leu Asn Trp Ala Arg Arg Val Thr Ile Ala Val Gln 600 605 610 gct gct agg gga att gag tac ttg cat ggc tat gct tgc cct cct gta 2046 Ala Ala Arg Gly Ile Glu Tyr Leu His Gly Tyr Ala Cys Pro Pro Val 615 620 625 630 att cac cgg gac atc aag tcg tca aac ata ttg att gat gag gat cac 2094 Ile His Arg Asp Ile Lys Ser Ser Asn Ile Leu Ile Asp Glu Asp His 635 640 645 aat gcc cgt gtt gct gac ttt ggt ctg tct ata ttg ggt cct gca gat 2142 Asn Ala Arg Val Ala Asp Phe Gly Leu Ser Ile Leu Gly Pro Ala Asp 650 655 660 agc ggt acc cca ctg tct gag ctg cca gca ggg act ctt ggc tac ctt 2190 Ser Gly Thr Pro Leu Ser Glu Leu Pro Ala Gly Thr Leu Gly Tyr Leu 665 670 675 gac cct gag tac tac cgt ctc cac tac ttg act aca aaa tct gat gtc 2238 Asp Pro Glu Tyr Tyr Arg Leu His Tyr Leu Thr Thr Lys Ser Asp Val 680 685 690 tac agc ttc ggg gtt gtt ctc ctg gag ata cta agt ggc agg aaa gcg 2286 Tyr Ser Phe Gly Val Val Leu Leu Glu Ile Leu Ser Gly Arg Lys Ala 695 700 705 710 atc gac atg cag ttc gag gag ggg aac att gtt gaa tgg gca gta cct 2334 Ile Asp Met Gln Phe Glu Glu Gly Asn Ile Val Glu Trp Ala Val Pro 715 720 725 ctg atc aaa gca ggg gac att ttt gcc atc ctt gat cca gtc tta tct 2382 Leu Ile Lys Ala Gly Asp Ile Phe Ala Ile Leu Asp Pro Val Leu Ser 730 735 740 cct ccc tca gac ctt gag gcc ctc aag aag att gct tct gtg gca tgt 2430 Pro Pro Ser Asp Leu Glu Ala Leu Lys Lys Ile Ala Ser Val Ala Cys 745 750 755 aag tgt gtc aga atg cga ggt aaa gat cgg cct tcc atg gat aag gtg 2478 Lys Cys Val Arg Met Arg Gly Lys Asp Arg Pro Ser Met Asp Lys Val 760 765 770 acg aca gct cta gag cac gcc ctt gca ctg ctg atg ggc agc ccc tgc 2526 Thr Thr Ala Leu Glu His Ala Leu Ala Leu Leu Met Gly Ser Pro Cys 775 780 785 790 atc gag cag ccc att cta ccg acc gag gtt gtt ctt gga agc agc cgc 2574 Ile Glu Gln Pro Ile Leu Pro Thr Glu Val Val Leu Gly Ser Ser Arg 795 800 805 atg cac aag gtg tca cag atg tcc tct aac cac tcc tgc tca gag aac 2622 Met His Lys Val Ser Gln Met Ser Ser Asn His Ser Cys Ser Glu Asn 810 815 820 gag ctt gct gac ggg gag gac cag ggg atc ggg tac agg gca cct tcc 2670 Glu Leu Ala Asp Gly Glu Asp Gln Gly Ile Gly Tyr Arg Ala Pro Ser 825 830 835 tgg ata act ttt cct agc gtg acc tca tca cag agg agg aaa tca tct 2718 Trp Ile Thr Phe Pro Ser Val Thr Ser Ser Gln Arg Arg Lys Ser Ser 840 845 850 gca tcc gaa gct gac atc gtt ggt cga agg gcc aca gac ggc agg aac 2766 Ala Ser Glu Ala Asp Ile Val Gly Arg Arg Ala Thr Asp Gly Arg Asn 855 860 865 870 gtc ggg agc agc ata ggt gac gga ctg cgg tca ctg gag gaa gaa atc 2814 Val Gly Ser Ser Ile Gly Asp Gly Leu Arg Ser Leu Glu Glu Glu Ile 875 880 885 gct cca gct tca cca caa gag aac ctg tac ttg cag cac aac ttc tga 2862 Ala Pro Ala Ser Pro Gln Glu Asn Leu Tyr Leu Gln His Asn Phe * 890 895 900 agaaattcag gagatgcagg aacctgagca gcgactttct gccattgttg agcagtattt 2922 taagttcggc ctttcatgcc ctgttgaagt gttcatacgt tctatataat agcagcctaa 2982 aaacagggag ctaattagta ctggaagaat tctttttagc gtccagaagc attcatctgt 3042 agattcgtat ggtccctttt attttcctgg tacaatttat cttctggtgg ctgcagttga 3102 tggattatcg tgtatcctta gcttgcaaaa ctgggtagtt tccttaatcc ttcagatcat 3162 ctctctggct cccgatgctg tttctgcatc ctaaatggca gtagctttgg gaaccaactg 3222 catgtctggg tcgggtcacc atgttcagca ttctttcgct tgtatccttg tggaaggttt 3282 cttcagttgc ttgatttgtg attcgcagca acaggctgtc tgcccaaaaa aaaaaaaaaa 3342 aaaa 3346 16 901 PRT Zea mays 16 Met Asp His Val Pro Ala Leu Val Leu Ala Gly Cys Cys Phe Leu Ala 1 5 10 15 Leu Leu Pro Gly Trp Ala Cys Gly Leu Gly Ser Met Ser Ser Ile Ala 20 25 30 Val Ser Tyr Gly Glu Asp Gly Pro Val Phe Cys Gly Leu Asn Ser Asp 35 40 45 Gly Ser His Leu Val Ala Cys Phe Gly Ala Asp Ala Ser Val Leu Tyr 50 55 60 Gly Ala Pro Pro Asn Ile Pro Phe Leu Gly Leu Thr Ala Gly Asp Gly 65 70 75 80 Phe Val Cys Gly Leu Leu Leu Asp Thr Arg Gln Pro Tyr Cys Trp Gly 85 90 95 Ser Asn Ser Tyr Val Lys Ser Gly Val Pro Gln Pro Met Val Glu Gly 100 105 110 Ala Arg Tyr Ser Glu Leu Ser Ala Gly Asp Asn His Leu Cys Ala Leu 115 120 125 Arg Ala Ala Gln Asp Gly Gly Arg Gly Ser Ser Ala Ala Thr Ser Leu 130 135 140 Ile Asp Cys Trp Gly Tyr Asn Met Thr Ala Thr His Ala Val Asp Glu 145 150 155 160 Ala Val Ser Thr Val Ser Ala Gly Ser Val Phe Asn Cys Gly Leu Phe 165 170 175 Ala Arg Asn Arg Thr Val Phe Cys Trp Gly Asp Glu Thr Val Ser Gly 180 185 190 Val Val Gly Leu Ala Pro Arg Asp Leu His Phe Gln Ser Ile Gly Ala 195 200 205 Gly Gly Tyr His Val Cys Gly Val Leu Glu Asn Ala Gln Val Phe Cys 210 215 220 Trp Gly Arg Ser Leu Glu Met Gln Gln Val Val Pro Ser Ser Ala Ile 225 230 235 240 Gly Asp Gly Asp Val Asn Ile Val Pro Met Asp Ala Met Ser Thr Val 245 250 255 Val Gly Gly Arg Phe His Ala Cys Gly Ile Arg Ser Leu Asp His Gln 260 265 270 Val Ala Cys Trp Gly Phe Thr Leu His Asn Ser Thr Ser Pro Pro Lys 275 280 285 Gly Leu Lys Met Tyr Ala Leu Val Ala Gly Asp Tyr Phe Thr Cys Gly 290 295 300 Val Pro Ala Glu Thr Ser Leu Met Pro Arg Cys Trp Gly Asn Ser Gly 305 310 315 320 Pro Leu Ala Leu Pro Met Ala Val Pro Pro Gly Ile Cys Val Pro Thr 325 330 335 Ala Cys Ser His Gly Tyr Tyr Glu Tyr Val Asn His Gly Glu Val Gly 340 345 350 Ser Ile Lys Val Cys Lys Pro Ala Asn Ser Arg Leu Cys Leu Pro Cys 355 360 365 Ser Thr Gly Cys Pro Glu Gly Leu Tyr Glu Ser Ser Pro Cys Asn Ala 370 375 380 Thr Ala Asp Arg Val Cys Gln Phe Asp Cys Leu Lys Cys Val Thr Asp 385 390 395 400 Glu Cys Leu Ser Phe Cys Leu Ser Gln Lys Arg Thr Lys Ser Arg Lys 405 410 415 Leu Met Ala Phe Gln Met Arg Ile Phe Val Ala Glu Ile Val Phe Ala 420 425 430 Val Val Leu Val Leu Ser Val Ser Val Thr Thr Cys Leu Tyr Val Arg 435 440 445 His Lys Leu Arg His Cys Gln Cys Ser Asn Arg Glu Leu Arg Leu Ala 450 455 460 Lys Ser Thr Ala Tyr Ser Phe Arg Lys Asp Asn Met Lys Ile Gln Pro 465 470 475 480 Asp Met Glu Asp Leu Lys Ile Arg Arg Ala Gln Glu Phe Ser Tyr Glu 485 490 495 Glu Leu Glu Gln Ala Thr Gly Gly Phe Ser Glu Asp Ser Gln Val Gly 500 505 510 Lys Gly Ser Phe Ser Cys Val Phe Lys Gly Ile Leu Arg Asp Gly Thr 515 520 525 Val Val Ala Val Lys Arg Ala Ile Lys Ala Ser Asp Val Lys Lys Ser 530 535 540 Ser Lys Glu Phe His Asn Glu Leu Asp Leu Leu Ser Arg Leu Asn His 545 550 555 560 Ala His Leu Leu Asn Leu Leu Gly Tyr Cys Glu Asp Gly Ser Glu Arg 565 570 575 Leu Leu Val Tyr Glu Phe Met Ala His Gly Ser Leu Tyr Gln His Leu 580 585 590 His Gly Lys Asp Pro Asn Leu Lys Lys Arg Leu Asn Trp Ala Arg Arg 595 600 605 Val Thr Ile Ala Val Gln Ala Ala Arg Gly Ile Glu Tyr Leu His Gly 610 615 620 Tyr Ala Cys Pro Pro Val Ile His Arg Asp Ile Lys Ser Ser Asn Ile 625 630 635 640 Leu Ile Asp Glu Asp His Asn Ala Arg Val Ala Asp Phe Gly Leu Ser 645 650 655 Ile Leu Gly Pro Ala Asp Ser Gly Thr Pro Leu Ser Glu Leu Pro Ala 660 665 670 Gly Thr Leu Gly Tyr Leu Asp Pro Glu Tyr Tyr Arg Leu His Tyr Leu 675 680 685 Thr Thr Lys Ser Asp Val Tyr Ser Phe Gly Val Val Leu Leu Glu Ile 690 695 700 Leu Ser Gly Arg Lys Ala Ile Asp Met Gln Phe Glu Glu Gly Asn Ile 705 710 715 720 Val Glu Trp Ala Val Pro Leu Ile Lys Ala Gly Asp Ile Phe Ala Ile 725 730 735 Leu Asp Pro Val Leu Ser Pro Pro Ser Asp Leu Glu Ala Leu Lys Lys 740 745 750 Ile Ala Ser Val Ala Cys Lys Cys Val Arg Met Arg Gly Lys Asp Arg 755 760 765 Pro Ser Met Asp Lys Val Thr Thr Ala Leu Glu His Ala Leu Ala Leu 770 775 780 Leu Met Gly Ser Pro Cys Ile Glu Gln Pro Ile Leu Pro Thr Glu Val 785 790 795 800 Val Leu Gly Ser Ser Arg Met His Lys Val Ser Gln Met Ser Ser Asn 805 810 815 His Ser Cys Ser Glu Asn Glu Leu Ala Asp Gly Glu Asp Gln Gly Ile 820 825 830 Gly Tyr Arg Ala Pro Ser Trp Ile Thr Phe Pro Ser Val Thr Ser Ser 835 840 845 Gln Arg Arg Lys Ser Ser Ala Ser Glu Ala Asp Ile Val Gly Arg Arg 850 855 860 Ala Thr Asp Gly Arg Asn Val Gly Ser Ser Ile Gly Asp Gly Leu Arg 865 870 875 880 Ser Leu Glu Glu Glu Ile Ala Pro Ala Ser Pro Gln Glu Asn Leu Tyr 885 890 895 Leu Gln His Asn Phe 900 17 1091 DNA Hordeum vulgare promoter (1)...(1091) nuc1 promoter 17 aagctttacg tttgagacgt atcatgtcgc ccgaggcgag gggacgcgag gggtacgcga 60 gaatttcctc cgtttgctag cagttgcaga tctatgcaat gattccgtgt gaagatggga 120 tgacggtaga tcctaagcgt gcgcatggag tacgcgttct ggaacaccta tgccggttgg 180 tactatcacc ttgatatgtg ggcagatgct gattagatgg tgctcattag tcatggcaca 240 tctttatact taggtgtagt gatttcgttt gccttgacga tgactttaga ttgatcgttg 300 tattcatttt gtgaggctgt gatgaataac taataaagat gactacatgc attattttga 360 tgcaaaggcc gggagcaatc tttcctttaa aaaaaaaact tcacatcaag acacttaaaa 420 tggcaacctg tcccttacta ctgctaaaca gcaagtgggt gagcatcttt atgcagtaat 480 ttgcacgggt acgccatccc atataaacag cactgtatga aagctgcatt cagttttctt 540 tctacatcag cacaggcagc ttcataaggt cttgtctggg cagaagaaag acgagtatcc 600 ggatattttt tgtggtctct gatttatctc cctacgtgca acagtacagt taaacgaagg 660 tgattaaact tacagtaagt cagtaacttg aactggtacc tcatcgcata aactgccact 720 gcattcagtt ttctttctat atcaacacag gcagctccat aactcataac caaccttaac 780 atccaaggtt attttcaggt gcaccagggc tagtgatagt acataaagtc tacttttgtt 840 cagaaaatga ataataccat ggcaaacttg gctacaggac acccaacatg acattttggg 900 ccatttaact tgctgcgaaa tactgaaata ctttactcgg ctgcattaca attatcaagg 960 tcaatgtcaa tattgccctc ggaactctaa tcctactgct atacaagtgc ccacactcag 1020 actttctgac gaagaacaga gccgccactc accacagcaa tcctcttccc ttcgccagca 1080 cgagcaagga g 1091 18 869 DNA Hordeum vulgare promoter (1)...(869) Ltp2 promoter TATA_signal (761)...(765) 18 gatctcgatg tgtagtctac gagaagggtt aaccgtctct tcgtgagaat

aaccgtggcc 60 taaaaataag ccgatgagga taaataaaat gtggtggtac agtacttcaa gaggtttact 120 catcaagagg atgcttttcc gatgagctct agtagtacat cggacctcac atacctccat 180 tgtggtgaaa tattttgtgc tcatttagtg atgggtaaat tttgtttatg tcactctagg 240 ttttgacatt tcagttttgc cactcttagg ttttgacaaa taatttccat tccgcggcaa 300 aagcaaaaca attttatttt acttttacca ctcttagctt tcacaatgta tcacaaatgc 360 cactctagaa attctgttta tgccacagaa tgtgaaaaaa aacactcact tatttgaagc 420 caaggtgttc atggcatgga aatgtgacat aaagtaacgt tcgtgtataa gaaaaaattg 480 tactcctcgt aacaagagac ggaaacatca tgagacaatc gcgtttggaa ggctttgcat 540 cacctttgga tgatgcgcat gaatggagtc gtctgcttgc tagccttcgc ctaccgccca 600 ctgagtccgg gcggcaacta ccatcggcga acgacccagc tgacctctac cgaccggact 660 tgaatgcgct accttcgtca gcgacgatgg ccgcgtacgc tggcgacgtg cccccgcatg 720 catggcggca catggcgagc tcagaccgtg cgtggctggc tacaaatacg taccccgtga 780 gtgccctagc tagaaactta cacctgcaac tgcgagagcg agcgtgtgag tgtagccgag 840 tagatcaccg tacgacgacg acgaggggc 869 19 713 DNA Zea mays promoter (1)...(713) end1 19 ggctggtaaa aaccattatt aactttaaca tcgaatcaaa actgacaaat tttatacttt 60 cacagagcag cagaaattta tacaatatga ttgaatacaa gatgtaggac ccgatggaga 120 gaattttttt gtctcctata tgcttgaata cccaacataa tatcttcgca gcatactatc 180 tatctaatag aaaaattata atatagttaa atacttaagt agtatctagt ggatagaatt 240 caatatctca tacatgcatg aggagtaata tctactagac atgcaacata tttttatcta 300 tctaatagaa tatatataat aaagttaaat attatatgca tcacctacta tatataattt 360 gatatctttt agatgtataa gggactaaga ataatatctc tagcacacat gcaatgcatt 420 atctatctaa atatattata taatagttaa atattaatta tacgtagtct aaacctacat 480 ataagcctac ccatccccac ttagagatct cagtgtcaca catagaccat acatctcact 540 tcgccaagaa aatttcgtca acagttgaag ttatacccat ggcaaaacta ctcttgggtt 600 tgctccttgc ccttgctatt ctagggacaa catcggctgc tggttgtgta caagaagggc 660 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agg 713 20 924 DNA Zea mays promoter (1)...(924) end2 20 agtttttggc agaaaaaatg atcaatgttt cacaaaccaa atatttttat aacttttgat 60 gaaagaagat caccacggtc atatctaggg gtggtaacaa attgcgatct aaatgtttct 120 tcataaaaaa taaggcttct taataaattt tagttcaaaa taaatacgaa taaagtctga 180 ttctaatctg attcgatcct taaattttat aatgcaaaat ttagagctca ttaccacctc 240 tagtcatatg tctagtctga ggtatatcca aaaagccctt tctctaaatt ccacacccaa 300 ctcagatgtt tgcaaataaa tactccgact ccaaaatgta ggtgaagtgc aactttctcc 360 attttatatc aacatttgtt attttttgtt taacatttca cactcaaaac taattaataa 420 aatacgtggt tgttgaacgt gcgcacatgt ctcccttaca ttatgttttt ttatttatgt 480 attattgttg ttttcctccg aacaacttgt caacatatca tcattggtct ttaatattta 540 tgaatatgga agcctagtta tttacacttg gctacacact agttgtagtt ttgccacttg 600 tctaacatgc aactctagta gttttgccac ttgcctggca cgcgactcta gtattgacac 660 ttgtatagca aataatgcca atacgacacc tggccttaca tgaaacatta tttttgacac 720 ttgtatacca tgcaacatta ccattgacat ttgtccatac acattatatc aaatatattg 780 agcgcatgtc acaaactcga tacaaagctg gatgaccctc cctcaccaca tctataaaaa 840 cccgagcgct actgtaaatc actcacaaca caacacatat cttttagtaa cctttcaata 900 ggcgtccccc aagaactagt aaac 924 21 2140 DNA Hordeum vugare promoter (1)...(2140) beps promoter 21 gagctcgact ccggcgacga tgcggcaacg gtgactgcaa caactgaccg gcgggggtgc 60 ggaaatccta gggggttcca ttcccagggc taatgcggcg gaagggggaa cctactggcg 120 gcgcaagaag cttgcgcttc tggccatggc ggaacgcgac agcgacgatg ctacgctacg 180 acgagcggat cgagcacgcg ggagagggca agagggtcta ctctgctcac ctaggttgca 240 ccgaaggcga gaacgaggcg cgcaggaaga agatgcttgg agcggcctgc tgcagtgaag 300 cttccgcgcc ggtgatgcga cgacggggcc gacgccatgg agggggctcg acctctccta 360 gcgcgggaac gggacaagga caatgccagc ttcctcgcgg gcgctcagac gccgcggaga 420 ggctgctgac gatgaaggag aaggcggaga cgacgaggat gatcccctgc tctcgctctg 480 aacctgacga tctctctctg aacttaccgg ggtagggaga tgatggagtg gagaagaggg 540 atggcggcgg cgataggagg ggcgcggaac cctaggattg gcagggctat ataggcgcga 600 cgggagatgg acctcgacgt ccgtgagggc cacggtggca cctcctctca cacgcggtct 660 cactctgacg gaaaagggag aaggagatgg cgacagtgcc gttagggggg agcttgggcc 720 acgcaggagg gtgatgggcc aggagagccc atctggcctt ctctctctct cactctcaaa 780 caagttctaa ttaaaatcaa acagaattga attaaaagcc aggggctagg gaaaggagtt 840 aaaaaaatcg ggcatctaaa atgttccccc atttacaaaa ataggcgtgg cattttagag 900 taaagaaaaa taaattggtt tatttgaaat tggctctgtt ttaaaataaa aacaaaggga 960 aaataaaacc cagaagagag tgttgccacc cgcaaatatt aaaaggattt tcaagagaag 1020 atgaacattt ttaatgggcc aaaacaaaaa ctttaaaaca accacaataa attgaaaaga 1080 gagggaaggg ttttatggtc atggtgcaac aagggttttg aagtggctct tgttgcaccc 1140 acactcatca cacaatcaac acaaagccac actcacaagg cactccactt tcaatcacca 1200 aaggtgcaac acaaacaaca agacaagaca aaggatgatg ccatgcatga tgtctaatgc 1260 acatggatga agacacaatg atgggctcac accacgtata ataccatatc aaggttgaca 1320 tgcaaggaaa gattacaagg agggggaaga atagcatagg ggctgtcaca aactattagt 1380 cctggagata atgtatgctg tcatgaatca tcaaaacttt caagggagca catgtgcttt 1440 cacatgtagc ctccatcatc gagggaggcc atggatcact agacaatcat aacttaggag 1500 acaccaaaat agtgccttca atggtgagtc tcattcatac ggcttccaat gcgcatgcct 1560 tgtggaggga ggaattgtaa tgcatgtgaa tgaggttgtt gcgaggtgtg tgttttggaa 1620 gtaccttaag cacgtcggta gtcactaacg aaaagtagcc tttttgtttt ttgttgtggg 1680 tcagctggat ccttcgagag ttgtaagatt ctatgcagca tggcatcttt gtcactttct 1740 tccataaaag ttgtacacca ttcttgggca tgttctggaa ataagagagc catgtatgat 1800 ttttgtatgg ctaaactcga tgccaataaa gcgaagagca tatctagaac acaatttttt 1860 tccagttcaa ctaatttcgt cgcacaaaac tatccatata tgttttttgt gtgtgaataa 1920 acttgttgcc aataaagcga agagcatatg tagtacgcca aaaactttac agcttgtcac 1980 atgcgaacta atttcgtcgc acatggatat tcatgtgctc ttttttgtac gtgcatatac 2040 ttccttcgcc tataaataaa agaagagttt ccttatgact tcaaaagtga actcacacat 2100 cactcaatat ctatatcctt ccattttata tccctcggtg 2140 22 36 DNA Artificial Sequence Sal A20 primer 22 tcgacccacg cgtccgaaaa aaaaaaaaaa aaaaaa 36 23 7110 DNA zea mays CDS (55)...(6532) dek1/calpain cDNA from B73 23 cttgcaggct atccatgatt tttgcctaca aaaggtgatt gaaaaggggg gagg atg 57 Met 1 gaa ggg gag gga cac cac gga gtt gtt ttg gca tgc agc atc tgt ggg 105 Glu Gly Glu Gly His His Gly Val Val Leu Ala Cys Ser Ile Cys Gly 5 10 15 ttc ctc ttc gct gtc ctt agc cct ttc agc ttt tgg gtt tta tgg gct 153 Phe Leu Phe Ala Val Leu Ser Pro Phe Ser Phe Trp Val Leu Trp Ala 20 25 30 gtg aat tgg agg cca tgg agg tta tac agt tgg ata tat gca agg aaa 201 Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Tyr Ala Arg Lys 35 40 45 tgg cca aca tat gtt caa gga cct caa ttg agc aca ctt tgc agc ctt 249 Trp Pro Thr Tyr Val Gln Gly Pro Gln Leu Ser Thr Leu Cys Ser Leu 50 55 60 65 ttg act ctt tgt gca tgg ctt gtt gtc att tcc cct ata gca gtt ctg 297 Leu Thr Leu Cys Ala Trp Leu Val Val Ile Ser Pro Ile Ala Val Leu 70 75 80 ctc gtg tgg gga agc gtc ctt att gct ctt atg gaa agg aat ata att 345 Leu Val Trp Gly Ser Val Leu Ile Ala Leu Met Glu Arg Asn Ile Ile 85 90 95 ggt tta gct gtt ata atg gcg ggt gtt gct ttg ctc ctg tca ttc tac 393 Gly Leu Ala Val Ile Met Ala Gly Val Ala Leu Leu Leu Ser Phe Tyr 100 105 110 tct ata atg ctc tgg tgg aga aca caa tgg caa agc tca aag gct gtt 441 Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Lys Ala Val 115 120 125 gct tac ctt ctc ctc ctg gca gta tgc ctg cta tgt gcc tac gat ttt 489 Ala Tyr Leu Leu Leu Leu Ala Val Cys Leu Leu Cys Ala Tyr Asp Phe 130 135 140 145 tgt gct att tat gtg aca gct ggt gct agt gct tcc gag ctt aat tct 537 Cys Ala Ile Tyr Val Thr Ala Gly Ala Ser Ala Ser Glu Leu Asn Ser 150 155 160 cca tca ggg ttc ttc ttc ggg gtg tct gta ata tca ttg gcc atc aat 585 Pro Ser Gly Phe Phe Phe Gly Val Ser Val Ile Ser Leu Ala Ile Asn 165 170 175 atg ctt ttt ata tgt aaa ata ctg ttt aat gta agt gga ttt gat gtt 633 Met Leu Phe Ile Cys Lys Ile Leu Phe Asn Val Ser Gly Phe Asp Val 180 185 190 gat gaa tat gtg cgg agg tca tac aaa ttt gcc tat tct gac tgt gtt 681 Asp Glu Tyr Val Arg Arg Ser Tyr Lys Phe Ala Tyr Ser Asp Cys Val 195 200 205 gaa gtg gct cct gtt tca tgc tct cct gag cca ccg gat cct agt gaa 729 Glu Val Ala Pro Val Ser Cys Ser Pro Glu Pro Pro Asp Pro Ser Glu 210 215 220 225 tta tac atg aca aaa tcc agc agg gtc aag cat tta ggg ctt ctg tac 777 Leu Tyr Met Thr Lys Ser Ser Arg Val Lys His Leu Gly Leu Leu Tyr 230 235 240 att agc tct ctg ctt gtg ctt gtt ggt tat tcc atc ttg tac ggt ctt 825 Ile Ser Ser Leu Leu Val Leu Val Gly Tyr Ser Ile Leu Tyr Gly Leu 245 250 255 acg tca aaa gaa gct cgt tgg ttg ggt gct tta act tca gtt gca gtt 873 Thr Ser Lys Glu Ala Arg Trp Leu Gly Ala Leu Thr Ser Val Ala Val 260 265 270 gtt atc ctt gac tgg aat ctg ggc tta tgt tca ttt aga ttt gag ctt 921 Val Ile Leu Asp Trp Asn Leu Gly Leu Cys Ser Phe Arg Phe Glu Leu 275 280 285 ctt aaa agt agg atg ata gtg tta ttt gtg gct gga aca tca agg gct 969 Leu Lys Ser Arg Met Ile Val Leu Phe Val Ala Gly Thr Ser Arg Ala 290 295 300 305 ttc ctt gta tcc ttt gga gtg cat tac tgg tac ctt ggc cat tgc atc 1017 Phe Leu Val Ser Phe Gly Val His Tyr Trp Tyr Leu Gly His Cys Ile 310 315 320 agc tat gct ttt gta gca tct gtg ctt tta tct gct gct gtt tct tcc 1065 Ser Tyr Ala Phe Val Ala Ser Val Leu Leu Ser Ala Ala Val Ser Ser 325 330 335 tgg ctt tct att tca aac ccc tca gtt gca agg ata gac gct cta aga 1113 Trp Leu Ser Ile Ser Asn Pro Ser Val Ala Arg Ile Asp Ala Leu Arg 340 345 350 agt acg gta ata aag cta cga gag gga ttt cga aga aaa gga caa aat 1161 Ser Thr Val Ile Lys Leu Arg Glu Gly Phe Arg Arg Lys Gly Gln Asn 355 360 365 agt tct tca aat tca tca gaa ggc tgt ggc tct agt gtg aag cgt agt 1209 Ser Ser Ser Asn Ser Ser Glu Gly Cys Gly Ser Ser Val Lys Arg Ser 370 375 380 385 agc ggt agt gtt gaa gct ggt caa aat ggt aat gca atg gat tct atg 1257 Ser Gly Ser Val Glu Ala Gly Gln Asn Gly Asn Ala Met Asp Ser Met 390 395 400 tac aga agc aac tca caa agc gat ggt gtc aat tgg agc agt att cct 1305 Tyr Arg Ser Asn Ser Gln Ser Asp Gly Val Asn Trp Ser Ser Ile Pro 405 410 415 ttt gat cga tca aac agt tgt caa gaa ggc cgg agc tcc gac aag aac 1353 Phe Asp Arg Ser Asn Ser Cys Gln Glu Gly Arg Ser Ser Asp Lys Asn 420 425 430 ata gat agt gca cgt gca agc tta gct cat cgg agt aat tca tgc tta 1401 Ile Asp Ser Ala Arg Ala Ser Leu Ala His Arg Ser Asn Ser Cys Leu 435 440 445 tct gcc gtc caa gac tct gaa acc gct gtt gtt tca gta gat agg cat 1449 Ser Ala Val Gln Asp Ser Glu Thr Ala Val Val Ser Val Asp Arg His 450 455 460 465 gga gat ccc att act tca ctt gtt tgt tct agc agt ggt ttg gaa agt 1497 Gly Asp Pro Ile Thr Ser Leu Val Cys Ser Ser Ser Gly Leu Glu Ser 470 475 480 cat ggc tgt gag cct agt gga tca gcc acc acc tca ggt aat caa cag 1545 His Gly Cys Glu Pro Ser Gly Ser Ala Thr Thr Ser Gly Asn Gln Gln 485 490 495 cta ttg gat ttg aac ctg gca gcg ata ttt cag gac aga tta aat gat 1593 Leu Leu Asp Leu Asn Leu Ala Ala Ile Phe Gln Asp Arg Leu Asn Asp 500 505 510 cca agg att tca tct atg cta aaa aag aat ggt gga ctt gga gat gta 1641 Pro Arg Ile Ser Ser Met Leu Lys Lys Asn Gly Gly Leu Gly Asp Val 515 520 525 gaa ctg gct aat ctt ctt cag gat aaa gga cta gat cca aat ttt tcg 1689 Glu Leu Ala Asn Leu Leu Gln Asp Lys Gly Leu Asp Pro Asn Phe Ser 530 535 540 545 tac atg ctg aaa gac aaa gtt atg gat cca cgt att tta gct ttg cta 1737 Tyr Met Leu Lys Asp Lys Val Met Asp Pro Arg Ile Leu Ala Leu Leu 550 555 560 cag agg agc agc ttg gat gca gat aga gag cat caa gat gac gta gat 1785 Gln Arg Ser Ser Leu Asp Ala Asp Arg Glu His Gln Asp Asp Val Asp 565 570 575 gtc aca gct act gat tca gat aga ttg gat acc act att gca aat cag 1833 Val Thr Ala Thr Asp Ser Asp Arg Leu Asp Thr Thr Ile Ala Asn Gln 580 585 590 att tct ctg tca gaa gaa cta agg aga agt ggt cta gaa aaa tgg ttg 1881 Ile Ser Leu Ser Glu Glu Leu Arg Arg Ser Gly Leu Glu Lys Trp Leu 595 600 605 aac att tca agg cta ata ttc cat cat tta gct gga tct cca ata cgt 1929 Asn Ile Ser Arg Leu Ile Phe His His Leu Ala Gly Ser Pro Ile Arg 610 615 620 625 gct ttt att gtt ttc aca gta atg ttt ata ata gag act gct act gtg 1977 Ala Phe Ile Val Phe Thr Val Met Phe Ile Ile Glu Thr Ala Thr Val 630 635 640 gct atc tat cgg cca gag acc atc aag gtg ata aat gca aca cat gaa 2025 Ala Ile Tyr Arg Pro Glu Thr Ile Lys Val Ile Asn Ala Thr His Glu 645 650 655 cag ttt gaa ttt ggt ttc tcg ata ctg ctt ctg tca cca gtt gtc tgc 2073 Gln Phe Glu Phe Gly Phe Ser Ile Leu Leu Leu Ser Pro Val Val Cys 660 665 670 tcc att atg gca ttc att tgg tct ctg cgt gct gaa gaa atg ttg atg 2121 Ser Ile Met Ala Phe Ile Trp Ser Leu Arg Ala Glu Glu Met Leu Met 675 680 685 aca tcc aag ccc cag aag tat ggt ttc att gca tgg cta ctg agc aca 2169 Thr Ser Lys Pro Gln Lys Tyr Gly Phe Ile Ala Trp Leu Leu Ser Thr 690 695 700 705 tgt gtt ggt ttg ttt ctc tct ttc tta agc aaa tca tct gtt ata ttg 2217 Cys Val Gly Leu Phe Leu Ser Phe Leu Ser Lys Ser Ser Val Ile Leu 710 715 720 ggc ctg tct ctc acg gta cca ctt atg gtg gct tgc ctc tca ttt gct 2265 Gly Leu Ser Leu Thr Val Pro Leu Met Val Ala Cys Leu Ser Phe Ala 725 730 735 gtt ccc ata tgg ata cgc aat ggt tac agt ttc tgg att cct gga agg 2313 Val Pro Ile Trp Ile Arg Asn Gly Tyr Ser Phe Trp Ile Pro Gly Arg 740 745 750 gag ttt gca aat cgt gaa aat gtt agt caa gct cca gga gag aaa gag 2361 Glu Phe Ala Asn Arg Glu Asn Val Ser Gln Ala Pro Gly Glu Lys Glu 755 760 765 cgg gct ctc ttt gtt atc acc att gct gtt ttc act gca tca att att 2409 Arg Ala Leu Phe Val Ile Thr Ile Ala Val Phe Thr Ala Ser Ile Ile 770 775 780 785 ggc ctt ggt gca ata gtg tca gca aag cct tta gac gct cta ggc tat 2457 Gly Leu Gly Ala Ile Val Ser Ala Lys Pro Leu Asp Ala Leu Gly Tyr 790 795 800 aaa gga tgg gat gct gat aag aac agc tcc tat tct ccc tat gca aca 2505 Lys Gly Trp Asp Ala Asp Lys Asn Ser Ser Tyr Ser Pro Tyr Ala Thr 805 810 815 tca atg tat ctt gga tgg gca ttg tct tca aca att gct gtg att acc 2553 Ser Met Tyr Leu Gly Trp Ala Leu Ser Ser Thr Ile Ala Val Ile Thr 820 825 830 aca ggg ttg ata cct att gtt gct tgg ttt gca aca tac cgg ttt tca 2601 Thr Gly Leu Ile Pro Ile Val Ala Trp Phe Ala Thr Tyr Arg Phe Ser 835 840 845 cct tca tca gct ata tgt gtt ggc ctc ttt gca act gtt ctt gtg tct 2649 Pro Ser Ser Ala Ile Cys Val Gly Leu Phe Ala Thr Val Leu Val Ser 850 855 860 865 ttt tgc ggt gca tcc tac tgg gga gtg gta aat tca cga gag gat ggt 2697 Phe Cys Gly Ala Ser Tyr Trp Gly Val Val Asn Ser Arg Glu Asp Gly 870 875 880 gtt cct cta aag gct gat ttc ctt gca gca tta ctt ccc ttg ctt tgc 2745 Val Pro Leu Lys Ala Asp Phe Leu Ala Ala Leu Leu Pro Leu Leu Cys 885 890 895 att cca gca ttt ttc tca ctg ttc act ggg ctt tac aaa tgg aag gat 2793 Ile Pro Ala Phe Phe Ser Leu Phe Thr Gly Leu Tyr Lys Trp Lys Asp 900 905 910 gat gat tgg aag att tct cgt ggt gtt tac ctt ttt gtt ggc atg gga 2841 Asp Asp Trp Lys Ile Ser Arg Gly Val Tyr Leu Phe Val Gly Met Gly 915 920 925 atg ttg ctg ttg ttt ggt gca gtt gca gct gtt att gtc aca atc agg 2889 Met Leu Leu Leu Phe Gly Ala Val Ala Ala Val Ile Val Thr Ile Arg 930 935 940 945 ccc tgg act gtt gga gtt gct tgc ctc gta gcc att ctg ttc ctt gta 2937 Pro Trp Thr Val Gly Val Ala Cys Leu Val Ala Ile Leu Phe Leu Val 950 955 960 ttt gtt att ggg gtc atc cac tac tgg aca tct aac aac ttc tat cta 2985 Phe Val Ile Gly Val Ile His Tyr Trp Thr Ser Asn Asn Phe Tyr Leu 965 970 975 acg agg act cag atg ttg ctt gtt tgt tcc att gct ttt ctc tta gcc 3033 Thr Arg Thr Gln Met Leu Leu Val Cys Ser Ile Ala Phe Leu Leu Ala 980 985 990 ttg gct gcc ttc ctg atg ggt tta ttt cac gga aag cct ttt gtt gga 3081 Leu Ala Ala

Phe Leu Met Gly Leu Phe His Gly Lys Pro Phe Val Gly 995 1000 1005 gca tct ata ggt tat ttc tca ttt ata ttt ctt ctc act gga agg gct 3129 Ala Ser Ile Gly Tyr Phe Ser Phe Ile Phe Leu Leu Thr Gly Arg Ala 1010 1015 1020 1025 ttg act gtc ctt cta tca ccg cca atc gta gtg tat tcg cca aga gta 3177 Leu Thr Val Leu Leu Ser Pro Pro Ile Val Val Tyr Ser Pro Arg Val 1030 1035 1040 ttg cct gta tac gtt tat gat gct cat gca gac tct gct aaa aat gtt 3225 Leu Pro Val Tyr Val Tyr Asp Ala His Ala Asp Ser Ala Lys Asn Val 1045 1050 1055 agc tat gcc ttt ctt att ctg tat ggg att gca tta gca act gaa gtt 3273 Ser Tyr Ala Phe Leu Ile Leu Tyr Gly Ile Ala Leu Ala Thr Glu Val 1060 1065 1070 tgg ggt gtt att gct agt cta ata atg aat cca cca ttt gtt ggg gct 3321 Trp Gly Val Ile Ala Ser Leu Ile Met Asn Pro Pro Phe Val Gly Ala 1075 1080 1085 ggc gtt tct gct act act ctt gta att gct ttc agt ttt gct gtt tct 3369 Gly Val Ser Ala Thr Thr Leu Val Ile Ala Phe Ser Phe Ala Val Ser 1090 1095 1100 1105 cga cca tgc ctg act ctt aag atg atg gag gat gca gtt cat ttt ctc 3417 Arg Pro Cys Leu Thr Leu Lys Met Met Glu Asp Ala Val His Phe Leu 1110 1115 1120 agc aag gat aca gtt gtg caa gcg atg tca cgg tct gct aat aaa act 3465 Ser Lys Asp Thr Val Val Gln Ala Met Ser Arg Ser Ala Asn Lys Thr 1125 1130 1135 aga aat gct ata tct ggg act tac tca gca cct cag agg tcc gca agt 3513 Arg Asn Ala Ile Ser Gly Thr Tyr Ser Ala Pro Gln Arg Ser Ala Ser 1140 1145 1150 tct gct gct ctt ttg gtt gga gat cct gct ctt aca ttg gac agg gct 3561 Ser Ala Ala Leu Leu Val Gly Asp Pro Ala Leu Thr Leu Asp Arg Ala 1155 1160 1165 ggg aac ttt gtg ctt cct agg gct gat gtt atg aaa ctg aga gat cgt 3609 Gly Asn Phe Val Leu Pro Arg Ala Asp Val Met Lys Leu Arg Asp Arg 1170 1175 1180 1185 ttg aga aat gaa gaa att gct gca gga tct ttc tta tgt gga gta aaa 3657 Leu Arg Asn Glu Glu Ile Ala Ala Gly Ser Phe Leu Cys Gly Val Lys 1190 1195 1200 gat tgt tta cta att tgc ccc cag tcc ctg tca aac ata gat tat cgg 3705 Asp Cys Leu Leu Ile Cys Pro Gln Ser Leu Ser Asn Ile Asp Tyr Arg 1205 1210 1215 agg aat atg tgt gcc cat gca cgt att ttg gct ttg gaa gaa gca att 3753 Arg Asn Met Cys Ala His Ala Arg Ile Leu Ala Leu Glu Glu Ala Ile 1220 1225 1230 gat aca gaa tgg gtg tat atg tgg gac aaa ttt ggt ggt tat tta ctt 3801 Asp Thr Glu Trp Val Tyr Met Trp Asp Lys Phe Gly Gly Tyr Leu Leu 1235 1240 1245 ctg ttg ctt gga ttg act gcc aaa gct gaa caa ata cag gat gaa gtt 3849 Leu Leu Leu Gly Leu Thr Ala Lys Ala Glu Gln Ile Gln Asp Glu Val 1250 1255 1260 1265 cgt cta aga ctc ttt ttg gat agc ata ggc ctt tcc gat ttg agt gcc 3897 Arg Leu Arg Leu Phe Leu Asp Ser Ile Gly Leu Ser Asp Leu Ser Ala 1270 1275 1280 aaa gaa att aag aaa tgg atg cct gaa gat cgg agg caa ttt gag ctt 3945 Lys Glu Ile Lys Lys Trp Met Pro Glu Asp Arg Arg Gln Phe Glu Leu 1285 1290 1295 att caa gaa agc tac ata agg gaa aaa gaa atg gaa gag gag gct ttg 3993 Ile Gln Glu Ser Tyr Ile Arg Glu Lys Glu Met Glu Glu Glu Ala Leu 1300 1305 1310 atg caa aga cga gag gaa gaa ggg aag gga aga gaa agg agg agg gca 4041 Met Gln Arg Arg Glu Glu Glu Gly Lys Gly Arg Glu Arg Arg Arg Ala 1315 1320 1325 ttg cta gag aga gag gag cga aaa tgg aag gag ctc gaa ata tca ttg 4089 Leu Leu Glu Arg Glu Glu Arg Lys Trp Lys Glu Leu Glu Ile Ser Leu 1330 1335 1340 1345 ctt tct tcc att cca aat act gga agc agg gat gct gca gct atg gca 4137 Leu Ser Ser Ile Pro Asn Thr Gly Ser Arg Asp Ala Ala Ala Met Ala 1350 1355 1360 gca gct gtc aga gct gtt gga ggt gat tct gcc ctg gaa gat tct ttt 4185 Ala Ala Val Arg Ala Val Gly Gly Asp Ser Ala Leu Glu Asp Ser Phe 1365 1370 1375 gca aga gat agg gtc tct tca ata gcc aat cac ata cga aag gca caa 4233 Ala Arg Asp Arg Val Ser Ser Ile Ala Asn His Ile Arg Lys Ala Gln 1380 1385 1390 ttg gct cgg cga gca gaa cag act ggt att cca ggc act ata tgc ata 4281 Leu Ala Arg Arg Ala Glu Gln Thr Gly Ile Pro Gly Thr Ile Cys Ile 1395 1400 1405 ctc gat gat gaa ccg agg agt act ggt cgt cat tgt gga gaa ctt gac 4329 Leu Asp Asp Glu Pro Arg Ser Thr Gly Arg His Cys Gly Glu Leu Asp 1410 1415 1420 1425 ttg tgc ctc tgt caa agt caa aag gtt act ttg tct att gct gtc atg 4377 Leu Cys Leu Cys Gln Ser Gln Lys Val Thr Leu Ser Ile Ala Val Met 1430 1435 1440 gtt cag cct gta tct ggc cca gtg tgt ctt ttt gga agt gaa ttc caa 4425 Val Gln Pro Val Ser Gly Pro Val Cys Leu Phe Gly Ser Glu Phe Gln 1445 1450 1455 aag gtt tgt tgg gaa atc tta gtg gca gga tca gaa cag ggt atg gaa 4473 Lys Val Cys Trp Glu Ile Leu Val Ala Gly Ser Glu Gln Gly Met Glu 1460 1465 1470 gct gga caa gtt ggt ctt cga tta gta act aag ggt gaa agg atg act 4521 Ala Gly Gln Val Gly Leu Arg Leu Val Thr Lys Gly Glu Arg Met Thr 1475 1480 1485 act gtt gct aaa gag tgg aat att ggt gcg tct agt att gca gat ggc 4569 Thr Val Ala Lys Glu Trp Asn Ile Gly Ala Ser Ser Ile Ala Asp Gly 1490 1495 1500 1505 agg tgg cat ctt gtc act gta act tta gat gcc gac cta ggt gaa gca 4617 Arg Trp His Leu Val Thr Val Thr Leu Asp Ala Asp Leu Gly Glu Ala 1510 1515 1520 act tct ttc att gat gga gtt tat gat gga tat cag aat ggg ttg ccg 4665 Thr Ser Phe Ile Asp Gly Val Tyr Asp Gly Tyr Gln Asn Gly Leu Pro 1525 1530 1535 ttg cca aca gat aac ggt att tgg gaa cct gga act gat att tgg gtt 4713 Leu Pro Thr Asp Asn Gly Ile Trp Glu Pro Gly Thr Asp Ile Trp Val 1540 1545 1550 ggt gct agg cca ccc atg gac tta gat gcc ttt ggt agg tca gat agc 4761 Gly Ala Arg Pro Pro Met Asp Leu Asp Ala Phe Gly Arg Ser Asp Ser 1555 1560 1565 gaa ggt tct gac tca aag atg cag atc atg gat gct ttt cta tgg gga 4809 Glu Gly Ser Asp Ser Lys Met Gln Ile Met Asp Ala Phe Leu Trp Gly 1570 1575 1580 1585 aga tgt ctc agt gaa gat gag gtt act gtt tta cat act gcc atg tct 4857 Arg Cys Leu Ser Glu Asp Glu Val Thr Val Leu His Thr Ala Met Ser 1590 1595 1600 cct gct gag tat gga ttt ttt gac ctt gca ccc ggc gat gct tgg cat 4905 Pro Ala Glu Tyr Gly Phe Phe Asp Leu Ala Pro Gly Asp Ala Trp His 1605 1610 1615 gga agt tat tct gca agg gtg gat gac tgg gaa agc gaa gaa gct tat 4953 Gly Ser Tyr Ser Ala Arg Val Asp Asp Trp Glu Ser Glu Glu Ala Tyr 1620 1625 1630 gag ctt tat gat caa ggg gat gtc gaa tgg gat gga cag tac tca agt 5001 Glu Leu Tyr Asp Gln Gly Asp Val Glu Trp Asp Gly Gln Tyr Ser Ser 1635 1640 1645 ggt agg aaa cgt ccg gta cat gat gct gta gct att gac ctt gac tcc 5049 Gly Arg Lys Arg Pro Val His Asp Ala Val Ala Ile Asp Leu Asp Ser 1650 1655 1660 1665 ttt gct agg aga cca aga aaa cca agg ttt gag aca cgt gat gaa gtc 5097 Phe Ala Arg Arg Pro Arg Lys Pro Arg Phe Glu Thr Arg Asp Glu Val 1670 1675 1680 aac cag cgt atg ctt tct gtt gaa agg gct gtc agg gat gct ctt atc 5145 Asn Gln Arg Met Leu Ser Val Glu Arg Ala Val Arg Asp Ala Leu Ile 1685 1690 1695 gcg aaa gga gag aga aac ttc act gat caa gag ttc cct cca gag gat 5193 Ala Lys Gly Glu Arg Asn Phe Thr Asp Gln Glu Phe Pro Pro Glu Asp 1700 1705 1710 cgt tct tta ttt gta gat ccg atg aat cca cct ctg aaa ctg cag gtt 5241 Arg Ser Leu Phe Val Asp Pro Met Asn Pro Pro Leu Lys Leu Gln Val 1715 1720 1725 gtt tct gag tgg atg agg cct tct gac ata gca aag gat ata tct atc 5289 Val Ser Glu Trp Met Arg Pro Ser Asp Ile Ala Lys Asp Ile Ser Ile 1730 1735 1740 1745 agt tgt cag cct tgc ttg ttt tcg ggt tct gtg aat tcc tca gat gtg 5337 Ser Cys Gln Pro Cys Leu Phe Ser Gly Ser Val Asn Ser Ser Asp Val 1750 1755 1760 tgt cag ggt cgg ttg gga gac tgt tgg ttc cta agt gca gtc gca gtt 5385 Cys Gln Gly Arg Leu Gly Asp Cys Trp Phe Leu Ser Ala Val Ala Val 1765 1770 1775 tta act gag atg tct cgg ata tca gaa gtt ata atc act ccc gag tac 5433 Leu Thr Glu Met Ser Arg Ile Ser Glu Val Ile Ile Thr Pro Glu Tyr 1780 1785 1790 aat gat gaa ggg att tat aca gtc aga ttc tgt att cag ggt gag tgg 5481 Asn Asp Glu Gly Ile Tyr Thr Val Arg Phe Cys Ile Gln Gly Glu Trp 1795 1800 1805 gtg gcc gtg gtt gtt gat gat tgg att cct tgc gag tct ccg ggg aaa 5529 Val Ala Val Val Val Asp Asp Trp Ile Pro Cys Glu Ser Pro Gly Lys 1810 1815 1820 1825 cca gca ttt gct act agt aga aag caa aac gag ctt tgg gta tcc att 5577 Pro Ala Phe Ala Thr Ser Arg Lys Gln Asn Glu Leu Trp Val Ser Ile 1830 1835 1840 ctt gag aag gct tat gca aaa ctt cat ggc tct tat gag gca ttg gaa 5625 Leu Glu Lys Ala Tyr Ala Lys Leu His Gly Ser Tyr Glu Ala Leu Glu 1845 1850 1855 ggt ggg ctt gtt caa gat gct cta gtc gat ctc aca gga gga gct ggt 5673 Gly Gly Leu Val Gln Asp Ala Leu Val Asp Leu Thr Gly Gly Ala Gly 1860 1865 1870 gaa gag att gat atg cga agt cct caa gcc caa ctt gat ctt gct agt 5721 Glu Glu Ile Asp Met Arg Ser Pro Gln Ala Gln Leu Asp Leu Ala Ser 1875 1880 1885 gga aga ttg tgg tcg cag ttg ttg cat ttc aaa caa gaa ggt ttt ctt 5769 Gly Arg Leu Trp Ser Gln Leu Leu His Phe Lys Gln Glu Gly Phe Leu 1890 1895 1900 1905 ctt ggt gct gga agt cct tct gga tct gat gct cac atc tca tca agt 5817 Leu Gly Ala Gly Ser Pro Ser Gly Ser Asp Ala His Ile Ser Ser Ser 1910 1915 1920 ggc att gtt cag gga cat gcg tac tca att ttg cag gta aga gaa gtt 5865 Gly Ile Val Gln Gly His Ala Tyr Ser Ile Leu Gln Val Arg Glu Val 1925 1930 1935 gat ggc cac aaa ctc atc caa atc aga aat cca tgg gca aat gaa gtt 5913 Asp Gly His Lys Leu Ile Gln Ile Arg Asn Pro Trp Ala Asn Glu Val 1940 1945 1950 gaa tgg aat gga cca tgg tca gac tcg tca cca gag tgg acg gaa cgg 5961 Glu Trp Asn Gly Pro Trp Ser Asp Ser Ser Pro Glu Trp Thr Glu Arg 1955 1960 1965 atg aag cat aag ctc atg cat gtt cca cag tcg aag aat ggg gta ttc 6009 Met Lys His Lys Leu Met His Val Pro Gln Ser Lys Asn Gly Val Phe 1970 1975 1980 1985 tgg atg tct tgg caa gat ttt cag att cac ttt cgg tca ata tat gtt 6057 Trp Met Ser Trp Gln Asp Phe Gln Ile His Phe Arg Ser Ile Tyr Val 1990 1995 2000 tgt cgt gtt tat cca cct gag atg cgt tac tct gtc cat ggg caa tgg 6105 Cys Arg Val Tyr Pro Pro Glu Met Arg Tyr Ser Val His Gly Gln Trp 2005 2010 2015 cgt ggc tac aat gca ggt ggt tgc caa gat tat gac tcg tgg cac caa 6153 Arg Gly Tyr Asn Ala Gly Gly Cys Gln Asp Tyr Asp Ser Trp His Gln 2020 2025 2030 aat cca cag tat cga ctt aga gta aca gga cgt gat gca cta tac cct 6201 Asn Pro Gln Tyr Arg Leu Arg Val Thr Gly Arg Asp Ala Leu Tyr Pro 2035 2040 2045 gtt cac gtt ttt att acc ctt act cag ggt gtt ggt ttc tct aga aag 6249 Val His Val Phe Ile Thr Leu Thr Gln Gly Val Gly Phe Ser Arg Lys 2050 2055 2060 2065 acg aat ggt ttt cgg aac tac caa tct agc cat gat tct tca atg ttt 6297 Thr Asn Gly Phe Arg Asn Tyr Gln Ser Ser His Asp Ser Ser Met Phe 2070 2075 2080 tac att gga atg agg ata ctc aag aca cag ggc tgc cgt gct gct tac 6345 Tyr Ile Gly Met Arg Ile Leu Lys Thr Gln Gly Cys Arg Ala Ala Tyr 2085 2090 2095 aat atc tac atg cat gaa agc gct ggt gga aca gat tac gtt aac tcg 6393 Asn Ile Tyr Met His Glu Ser Ala Gly Gly Thr Asp Tyr Val Asn Ser 2100 2105 2110 agg gag ata tca tgc gaa ctg gtc ttg gat cct tat ccc aaa ggg tac 6441 Arg Glu Ile Ser Cys Glu Leu Val Leu Asp Pro Tyr Pro Lys Gly Tyr 2115 2120 2125 aca att gtg cca act acc atc cac cct ggg gag gaa gca cct ttt gtt 6489 Thr Ile Val Pro Thr Thr Ile His Pro Gly Glu Glu Ala Pro Phe Val 2130 2135 2140 2145 ttg tca gtt ttt tca aaa gca tca atc aga cta gag gct gtt t 6532 Leu Ser Val Phe Ser Lys Ala Ser Ile Arg Leu Glu Ala Val 2150 2155 agttcaagat tgagatccca tgtgtttgat ggtagctgcg tctgctgggc acccgtgcac 6592 gcaggatcca gctgtgggtt ctcgggaact agataatggg tataggaatt gcctcctgga 6652 caacttcaat caatcttgct gcatgcaagt acctaagttc ggttgcttgt tgcagatctg 6712 acaaacggca atgcttcttg tgctgaaggg aaaggagaga aggcatgatc catggttctt 6772 tggtagctgc gcaaagtgca gggtgagagg cttggttcaa tgtttgtaga tagccgtggt 6832 aactgacctg gtagcccatc ctatgtatag gtgtcccgtt taccctgtaa atgctataga 6892 gttaggttag gtagcctgtc gttcctgtta acgcataggg ctcttatgca gctgtgaaat 6952 gtcttgttgg caagctgcag ttttgctgat ttgagcgtgg agtagtcggc catagctgtt 7012 cccattggtt tgccctgtat gtaatcggaa tctgatgtca ttcaatgaac ctattttttg 7072 ggtgccatgc gaagctgtct aaaaaaaaaa aaaaaaaa 7110 24 2159 PRT zea mays 24 Met Glu Gly Glu Gly His His Gly Val Val Leu Ala Cys Ser Ile Cys 1 5 10 15 Gly Phe Leu Phe Ala Val Leu Ser Pro Phe Ser Phe Trp Val Leu Trp 20 25 30 Ala Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Tyr Ala Arg 35 40 45 Lys Trp Pro Thr Tyr Val Gln Gly Pro Gln Leu Ser Thr Leu Cys Ser 50 55 60 Leu Leu Thr Leu Cys Ala Trp Leu Val Val Ile Ser Pro Ile Ala Val 65 70 75 80 Leu Leu Val Trp Gly Ser Val Leu Ile Ala Leu Met Glu Arg Asn Ile 85 90 95 Ile Gly Leu Ala Val Ile Met Ala Gly Val Ala Leu Leu Leu Ser Phe 100 105 110 Tyr Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Lys Ala 115 120 125 Val Ala Tyr Leu Leu Leu Leu Ala Val Cys Leu Leu Cys Ala Tyr Asp 130 135 140 Phe Cys Ala Ile Tyr Val Thr Ala Gly Ala Ser Ala Ser Glu Leu Asn 145 150 155 160 Ser Pro Ser Gly Phe Phe Phe Gly Val Ser Val Ile Ser Leu Ala Ile 165 170 175 Asn Met Leu Phe Ile Cys Lys Ile Leu Phe Asn Val Ser Gly Phe Asp 180 185 190 Val Asp Glu Tyr Val Arg Arg Ser Tyr Lys Phe Ala Tyr Ser Asp Cys 195 200 205 Val Glu Val Ala Pro Val Ser Cys Ser Pro Glu Pro Pro Asp Pro Ser 210 215 220 Glu Leu Tyr Met Thr Lys Ser Ser Arg Val Lys His Leu Gly Leu Leu 225 230 235 240 Tyr Ile Ser Ser Leu Leu Val Leu Val Gly Tyr Ser Ile Leu Tyr Gly 245 250 255 Leu Thr Ser Lys Glu Ala Arg Trp Leu Gly Ala Leu Thr Ser Val Ala 260 265 270 Val Val Ile Leu Asp Trp Asn Leu Gly Leu Cys Ser Phe Arg Phe Glu 275 280 285 Leu Leu Lys Ser Arg Met Ile Val Leu Phe Val Ala Gly Thr Ser Arg 290 295 300 Ala Phe Leu Val Ser Phe Gly Val His Tyr Trp Tyr Leu Gly His Cys 305 310 315 320 Ile Ser Tyr Ala Phe Val Ala Ser Val Leu Leu Ser Ala Ala Val Ser 325 330 335 Ser Trp Leu Ser Ile Ser Asn Pro Ser Val Ala Arg Ile Asp Ala Leu 340 345 350 Arg Ser Thr Val Ile Lys Leu Arg Glu Gly Phe Arg Arg Lys Gly Gln 355 360 365 Asn Ser Ser Ser Asn Ser Ser Glu Gly Cys Gly Ser Ser Val Lys Arg 370 375 380 Ser Ser Gly Ser Val Glu Ala Gly Gln Asn Gly Asn Ala Met Asp Ser 385 390 395 400 Met Tyr Arg Ser Asn Ser Gln Ser Asp Gly Val Asn Trp Ser Ser Ile 405 410 415 Pro Phe Asp Arg Ser Asn Ser Cys Gln Glu Gly Arg Ser Ser Asp Lys 420 425 430 Asn Ile Asp Ser Ala Arg Ala Ser Leu Ala His Arg Ser Asn Ser Cys 435 440 445 Leu Ser Ala Val Gln Asp Ser Glu Thr Ala Val Val Ser Val Asp Arg 450 455 460 His Gly

Asp Pro Ile Thr Ser Leu Val Cys Ser Ser Ser Gly Leu Glu 465 470 475 480 Ser His Gly Cys Glu Pro Ser Gly Ser Ala Thr Thr Ser Gly Asn Gln 485 490 495 Gln Leu Leu Asp Leu Asn Leu Ala Ala Ile Phe Gln Asp Arg Leu Asn 500 505 510 Asp Pro Arg Ile Ser Ser Met Leu Lys Lys Asn Gly Gly Leu Gly Asp 515 520 525 Val Glu Leu Ala Asn Leu Leu Gln Asp Lys Gly Leu Asp Pro Asn Phe 530 535 540 Ser Tyr Met Leu Lys Asp Lys Val Met Asp Pro Arg Ile Leu Ala Leu 545 550 555 560 Leu Gln Arg Ser Ser Leu Asp Ala Asp Arg Glu His Gln Asp Asp Val 565 570 575 Asp Val Thr Ala Thr Asp Ser Asp Arg Leu Asp Thr Thr Ile Ala Asn 580 585 590 Gln Ile Ser Leu Ser Glu Glu Leu Arg Arg Ser Gly Leu Glu Lys Trp 595 600 605 Leu Asn Ile Ser Arg Leu Ile Phe His His Leu Ala Gly Ser Pro Ile 610 615 620 Arg Ala Phe Ile Val Phe Thr Val Met Phe Ile Ile Glu Thr Ala Thr 625 630 635 640 Val Ala Ile Tyr Arg Pro Glu Thr Ile Lys Val Ile Asn Ala Thr His 645 650 655 Glu Gln Phe Glu Phe Gly Phe Ser Ile Leu Leu Leu Ser Pro Val Val 660 665 670 Cys Ser Ile Met Ala Phe Ile Trp Ser Leu Arg Ala Glu Glu Met Leu 675 680 685 Met Thr Ser Lys Pro Gln Lys Tyr Gly Phe Ile Ala Trp Leu Leu Ser 690 695 700 Thr Cys Val Gly Leu Phe Leu Ser Phe Leu Ser Lys Ser Ser Val Ile 705 710 715 720 Leu Gly Leu Ser Leu Thr Val Pro Leu Met Val Ala Cys Leu Ser Phe 725 730 735 Ala Val Pro Ile Trp Ile Arg Asn Gly Tyr Ser Phe Trp Ile Pro Gly 740 745 750 Arg Glu Phe Ala Asn Arg Glu Asn Val Ser Gln Ala Pro Gly Glu Lys 755 760 765 Glu Arg Ala Leu Phe Val Ile Thr Ile Ala Val Phe Thr Ala Ser Ile 770 775 780 Ile Gly Leu Gly Ala Ile Val Ser Ala Lys Pro Leu Asp Ala Leu Gly 785 790 795 800 Tyr Lys Gly Trp Asp Ala Asp Lys Asn Ser Ser Tyr Ser Pro Tyr Ala 805 810 815 Thr Ser Met Tyr Leu Gly Trp Ala Leu Ser Ser Thr Ile Ala Val Ile 820 825 830 Thr Thr Gly Leu Ile Pro Ile Val Ala Trp Phe Ala Thr Tyr Arg Phe 835 840 845 Ser Pro Ser Ser Ala Ile Cys Val Gly Leu Phe Ala Thr Val Leu Val 850 855 860 Ser Phe Cys Gly Ala Ser Tyr Trp Gly Val Val Asn Ser Arg Glu Asp 865 870 875 880 Gly Val Pro Leu Lys Ala Asp Phe Leu Ala Ala Leu Leu Pro Leu Leu 885 890 895 Cys Ile Pro Ala Phe Phe Ser Leu Phe Thr Gly Leu Tyr Lys Trp Lys 900 905 910 Asp Asp Asp Trp Lys Ile Ser Arg Gly Val Tyr Leu Phe Val Gly Met 915 920 925 Gly Met Leu Leu Leu Phe Gly Ala Val Ala Ala Val Ile Val Thr Ile 930 935 940 Arg Pro Trp Thr Val Gly Val Ala Cys Leu Val Ala Ile Leu Phe Leu 945 950 955 960 Val Phe Val Ile Gly Val Ile His Tyr Trp Thr Ser Asn Asn Phe Tyr 965 970 975 Leu Thr Arg Thr Gln Met Leu Leu Val Cys Ser Ile Ala Phe Leu Leu 980 985 990 Ala Leu Ala Ala Phe Leu Met Gly Leu Phe His Gly Lys Pro Phe Val 995 1000 1005 Gly Ala Ser Ile Gly Tyr Phe Ser Phe Ile Phe Leu Leu Thr Gly Arg 1010 1015 1020 Ala Leu Thr Val Leu Leu Ser Pro Pro Ile Val Val Tyr Ser Pro Arg 1025 1030 1035 1040 Val Leu Pro Val Tyr Val Tyr Asp Ala His Ala Asp Ser Ala Lys Asn 1045 1050 1055 Val Ser Tyr Ala Phe Leu Ile Leu Tyr Gly Ile Ala Leu Ala Thr Glu 1060 1065 1070 Val Trp Gly Val Ile Ala Ser Leu Ile Met Asn Pro Pro Phe Val Gly 1075 1080 1085 Ala Gly Val Ser Ala Thr Thr Leu Val Ile Ala Phe Ser Phe Ala Val 1090 1095 1100 Ser Arg Pro Cys Leu Thr Leu Lys Met Met Glu Asp Ala Val His Phe 1105 1110 1115 1120 Leu Ser Lys Asp Thr Val Val Gln Ala Met Ser Arg Ser Ala Asn Lys 1125 1130 1135 Thr Arg Asn Ala Ile Ser Gly Thr Tyr Ser Ala Pro Gln Arg Ser Ala 1140 1145 1150 Ser Ser Ala Ala Leu Leu Val Gly Asp Pro Ala Leu Thr Leu Asp Arg 1155 1160 1165 Ala Gly Asn Phe Val Leu Pro Arg Ala Asp Val Met Lys Leu Arg Asp 1170 1175 1180 Arg Leu Arg Asn Glu Glu Ile Ala Ala Gly Ser Phe Leu Cys Gly Val 1185 1190 1195 1200 Lys Asp Cys Leu Leu Ile Cys Pro Gln Ser Leu Ser Asn Ile Asp Tyr 1205 1210 1215 Arg Arg Asn Met Cys Ala His Ala Arg Ile Leu Ala Leu Glu Glu Ala 1220 1225 1230 Ile Asp Thr Glu Trp Val Tyr Met Trp Asp Lys Phe Gly Gly Tyr Leu 1235 1240 1245 Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala Glu Gln Ile Gln Asp Glu 1250 1255 1260 Val Arg Leu Arg Leu Phe Leu Asp Ser Ile Gly Leu Ser Asp Leu Ser 1265 1270 1275 1280 Ala Lys Glu Ile Lys Lys Trp Met Pro Glu Asp Arg Arg Gln Phe Glu 1285 1290 1295 Leu Ile Gln Glu Ser Tyr Ile Arg Glu Lys Glu Met Glu Glu Glu Ala 1300 1305 1310 Leu Met Gln Arg Arg Glu Glu Glu Gly Lys Gly Arg Glu Arg Arg Arg 1315 1320 1325 Ala Leu Leu Glu Arg Glu Glu Arg Lys Trp Lys Glu Leu Glu Ile Ser 1330 1335 1340 Leu Leu Ser Ser Ile Pro Asn Thr Gly Ser Arg Asp Ala Ala Ala Met 1345 1350 1355 1360 Ala Ala Ala Val Arg Ala Val Gly Gly Asp Ser Ala Leu Glu Asp Ser 1365 1370 1375 Phe Ala Arg Asp Arg Val Ser Ser Ile Ala Asn His Ile Arg Lys Ala 1380 1385 1390 Gln Leu Ala Arg Arg Ala Glu Gln Thr Gly Ile Pro Gly Thr Ile Cys 1395 1400 1405 Ile Leu Asp Asp Glu Pro Arg Ser Thr Gly Arg His Cys Gly Glu Leu 1410 1415 1420 Asp Leu Cys Leu Cys Gln Ser Gln Lys Val Thr Leu Ser Ile Ala Val 1425 1430 1435 1440 Met Val Gln Pro Val Ser Gly Pro Val Cys Leu Phe Gly Ser Glu Phe 1445 1450 1455 Gln Lys Val Cys Trp Glu Ile Leu Val Ala Gly Ser Glu Gln Gly Met 1460 1465 1470 Glu Ala Gly Gln Val Gly Leu Arg Leu Val Thr Lys Gly Glu Arg Met 1475 1480 1485 Thr Thr Val Ala Lys Glu Trp Asn Ile Gly Ala Ser Ser Ile Ala Asp 1490 1495 1500 Gly Arg Trp His Leu Val Thr Val Thr Leu Asp Ala Asp Leu Gly Glu 1505 1510 1515 1520 Ala Thr Ser Phe Ile Asp Gly Val Tyr Asp Gly Tyr Gln Asn Gly Leu 1525 1530 1535 Pro Leu Pro Thr Asp Asn Gly Ile Trp Glu Pro Gly Thr Asp Ile Trp 1540 1545 1550 Val Gly Ala Arg Pro Pro Met Asp Leu Asp Ala Phe Gly Arg Ser Asp 1555 1560 1565 Ser Glu Gly Ser Asp Ser Lys Met Gln Ile Met Asp Ala Phe Leu Trp 1570 1575 1580 Gly Arg Cys Leu Ser Glu Asp Glu Val Thr Val Leu His Thr Ala Met 1585 1590 1595 1600 Ser Pro Ala Glu Tyr Gly Phe Phe Asp Leu Ala Pro Gly Asp Ala Trp 1605 1610 1615 His Gly Ser Tyr Ser Ala Arg Val Asp Asp Trp Glu Ser Glu Glu Ala 1620 1625 1630 Tyr Glu Leu Tyr Asp Gln Gly Asp Val Glu Trp Asp Gly Gln Tyr Ser 1635 1640 1645 Ser Gly Arg Lys Arg Pro Val His Asp Ala Val Ala Ile Asp Leu Asp 1650 1655 1660 Ser Phe Ala Arg Arg Pro Arg Lys Pro Arg Phe Glu Thr Arg Asp Glu 1665 1670 1675 1680 Val Asn Gln Arg Met Leu Ser Val Glu Arg Ala Val Arg Asp Ala Leu 1685 1690 1695 Ile Ala Lys Gly Glu Arg Asn Phe Thr Asp Gln Glu Phe Pro Pro Glu 1700 1705 1710 Asp Arg Ser Leu Phe Val Asp Pro Met Asn Pro Pro Leu Lys Leu Gln 1715 1720 1725 Val Val Ser Glu Trp Met Arg Pro Ser Asp Ile Ala Lys Asp Ile Ser 1730 1735 1740 Ile Ser Cys Gln Pro Cys Leu Phe Ser Gly Ser Val Asn Ser Ser Asp 1745 1750 1755 1760 Val Cys Gln Gly Arg Leu Gly Asp Cys Trp Phe Leu Ser Ala Val Ala 1765 1770 1775 Val Leu Thr Glu Met Ser Arg Ile Ser Glu Val Ile Ile Thr Pro Glu 1780 1785 1790 Tyr Asn Asp Glu Gly Ile Tyr Thr Val Arg Phe Cys Ile Gln Gly Glu 1795 1800 1805 Trp Val Ala Val Val Val Asp Asp Trp Ile Pro Cys Glu Ser Pro Gly 1810 1815 1820 Lys Pro Ala Phe Ala Thr Ser Arg Lys Gln Asn Glu Leu Trp Val Ser 1825 1830 1835 1840 Ile Leu Glu Lys Ala Tyr Ala Lys Leu His Gly Ser Tyr Glu Ala Leu 1845 1850 1855 Glu Gly Gly Leu Val Gln Asp Ala Leu Val Asp Leu Thr Gly Gly Ala 1860 1865 1870 Gly Glu Glu Ile Asp Met Arg Ser Pro Gln Ala Gln Leu Asp Leu Ala 1875 1880 1885 Ser Gly Arg Leu Trp Ser Gln Leu Leu His Phe Lys Gln Glu Gly Phe 1890 1895 1900 Leu Leu Gly Ala Gly Ser Pro Ser Gly Ser Asp Ala His Ile Ser Ser 1905 1910 1915 1920 Ser Gly Ile Val Gln Gly His Ala Tyr Ser Ile Leu Gln Val Arg Glu 1925 1930 1935 Val Asp Gly His Lys Leu Ile Gln Ile Arg Asn Pro Trp Ala Asn Glu 1940 1945 1950 Val Glu Trp Asn Gly Pro Trp Ser Asp Ser Ser Pro Glu Trp Thr Glu 1955 1960 1965 Arg Met Lys His Lys Leu Met His Val Pro Gln Ser Lys Asn Gly Val 1970 1975 1980 Phe Trp Met Ser Trp Gln Asp Phe Gln Ile His Phe Arg Ser Ile Tyr 1985 1990 1995 2000 Val Cys Arg Val Tyr Pro Pro Glu Met Arg Tyr Ser Val His Gly Gln 2005 2010 2015 Trp Arg Gly Tyr Asn Ala Gly Gly Cys Gln Asp Tyr Asp Ser Trp His 2020 2025 2030 Gln Asn Pro Gln Tyr Arg Leu Arg Val Thr Gly Arg Asp Ala Leu Tyr 2035 2040 2045 Pro Val His Val Phe Ile Thr Leu Thr Gln Gly Val Gly Phe Ser Arg 2050 2055 2060 Lys Thr Asn Gly Phe Arg Asn Tyr Gln Ser Ser His Asp Ser Ser Met 2065 2070 2075 2080 Phe Tyr Ile Gly Met Arg Ile Leu Lys Thr Gln Gly Cys Arg Ala Ala 2085 2090 2095 Tyr Asn Ile Tyr Met His Glu Ser Ala Gly Gly Thr Asp Tyr Val Asn 2100 2105 2110 Ser Arg Glu Ile Ser Cys Glu Leu Val Leu Asp Pro Tyr Pro Lys Gly 2115 2120 2125 Tyr Thr Ile Val Pro Thr Thr Ile His Pro Gly Glu Glu Ala Pro Phe 2130 2135 2140 Val Leu Ser Val Phe Ser Lys Ala Ser Ile Arg Leu Glu Ala Val 2145 2150 2155 25 25411 DNA zea mays CDS (2682)...(23733) dek1/calpain genomic DNA from Mo17 misc_feature (1)...(25411) n = A,T,C or G 25 accgttggtg gaggctgact gtcgtatggt gcaccggaca gtccggtgca caccggacat 60 gtccggtgca ccagccacgt caccaatgcc gttggattcc aaccgttgga gcttctgtct 120 tctgggcccg cctggatgtc cggtgcacac cggacatgta ctgttcaatg tccggtgcgc 180 cagtatgggc gtgcctgact tctgcgcgct tctggcgcgc attgaatgcg cctgcaggtg 240 accgttggcg cgaagtagcc gttgctccgc agttgcaccg gacagtccgg tgtacaccgg 300 acatgtccgg tgaattatag cggagcagct gctgcgcgtt cccgaggctg gcgagttccg 360 gaggccgctc ttccttggag caccggacac tgtccggtgt acaccagaca gtccggtgaa 420 ttatagcgga gtgcctctgg aaattcccga aggtgtcaag tttgagttgg agtcctctgg 480 tgcaccggac actgtccggt gtacaccgga cagttcggtg cccccagacc agaggtgcct 540 tcggttgcct ctttgctcct ttgttgaatc caaaacttat ctttttattg gctgagtgtg 600 aaccttttac acatgtataa tctatacact tgggcaaact agttagtcca attatttgtg 660 ttgggtattt caaccaccaa aattatttag gaactaggtg taagcctaat tccctttcac 720 atgcgcacct tgttgaccgc acgccacctc caccaaggcc gccgccccca cgaccacacg 780 ccgccactcc gctgcggtcc cccgaaggct ccctccccga gactgccctc cacctcgcgt 840 gcctcgttga cgagtggcct catgtcgtgg ggtccgtgcg cttgaccgcg agccgagttt 900 cgcttcgaca tcatttcgga cggctcgcag atgttgtcct ccatctcata acagtatgac 960 acacatttgt ataaaagtta ttgtggtatt ataggtttcc gttgcaacac acggacactc 1020 acctagtaaa ttgtagaatg caaagcgagc actacaacat aaaatcttta ttattagagc 1080 acggaaaaca atttttaacg aatatctttt ttatgcgtaa caatgtttct gtttcacata 1140 aaccactgtc cagcataaaa atatttcatg ttcacttcaa aaatcacaat acaaataaaa 1200 acgtttctac tttaggaaaa tattattatg gctttatgcc attttttttg gcttgtgaaa 1260 ttatatgtta ctgtaaaaat gcttccgatt taatagaaaa atggtggacc tcaataaaac 1320 atataaaaat ttatgttttt gctactctct ctatcccaaa ttaaaattta ttttagcttt 1380 tatcggattc atacattagt tatatatata tatataggtt cgtaatcatc catatgaact 1440 gaatatagaa atatagagct aaaactactt ttatttaagg ggatgaggga gtatatttta 1500 atttcgttat tatctaaaat caatgttgta ctgacttcca agaagaacta taaaaatttt 1560 aaaaataaaa aataataaat aaatttccga agaaatgaaa caaaccgccc tgtagagccc 1620 gtgcgggtgc gcccaacaac cccttccgct gccttcccac ttccgtccgc agtccgctgc 1680 attgctcgcg tcgcgtgtgt tctgtctgtc actctcgccc agtcgccctc ctcttgctct 1740 ccgacgactg gtgggctgcc gctgccgccg ccgccgccct acgccaggtg ctgaggcttt 1800 catcggtctc ttcgtcggtg tctttgccgg cgtcgagcac ccgccaggta ctgccacggt 1860 cctacccctt ctcttccctt catgctgtgc gaggctgagc accgaaaccc taacctcttt 1920 agctatttga caagcctcct accttcgaag cttttgcaaa aattattggg tgtacatgtg 1980 tacacccatg tccctttact ggttccgccc ccgtaaagac taagctgaaa ccatggaaac 2040 aaattcagga atttcgagtt atatctaata gcattgatgg aattaacatc aagtcgatca 2100 caaggaagct aatttctagt ttccccatgc acaagcaacc caacccgtct ttgagtaatt 2160 tctaatctgt atcaaacatt gaggctacat atctggtaga tcccttttgt tgtgtctgga 2220 agcagatttg aactggcatg gatgtagacc gttaaaggtg gtaccaagca aaatggactt 2280 gttaatacat tcttgtgtgc ataaacttac catttcttac tagaaggaat agtcaaatta 2340 tctccaatta aacctaattc aaaacagttc aatgtaatgg agccaacttg ttgattatta 2400 agtcaccaaa ttacataggt ggagataaac tttggggagt tttaactgat ggcctgtttg 2460 gtacatgcca atgctctgta tatggtaaca cactaggcat ttgctagtta ctactaggat 2520 tagccaaatc attatcatat ttcttgggat ctgtgaattg gttacttaac actgaactgc 2580 atgctcgagt gtcatttagt gctctttgct cttatgttgt accttttctt gcaggctatc 2640 catgattttt gcctacaaaa ggtgattgaa aaggggggag g atg gaa ggg gag gga 2696 Met Glu Gly Glu Gly 1 5 cac cac gga gtt gtt ttg gca tgc agc atc tgt ggg ttc ctc ttc gct 2744 His His Gly Val Val Leu Ala Cys Ser Ile Cys Gly Phe Leu Phe Ala 10 15 20 gtc ctt agc cct ttc agc ttt tgg gtt tta tgg gct gtg aat tgg cgg 2792 Val Leu Ser Pro Phe Ser Phe Trp Val Leu Trp Ala Val Asn Trp Arg 25 30 35 cca tgg agg tta tac agg tat gct gca gaa gtt tta tat ctt tct ctc 2840 Pro Trp Arg Leu Tyr Arg Tyr Ala Ala Glu Val Leu Tyr Leu Ser Leu 40 45 50 tcg ggc tca act aat att tta ctt ttt tgt tgt tgt ctg tta tct cgt 2888 Ser Gly Ser Thr Asn Ile Leu Leu Phe Cys Cys Cys Leu Leu Ser Arg 55 60 65 ttg tat tct gtc ggt tct cac taa aac tgc atg cct ttt ctt tgt aca 2936 Leu Tyr Ser Val Gly Ser His * Asn Cys Met Pro Phe Leu Cys Thr 70 75 80 att gac tac cat aaa caa agc agg aaa ccc att ttt tgt ttt tgt att 2984 Ile Asp Tyr His Lys Gln Ser Arg Lys Pro Ile Phe Cys Phe Cys Ile 85 90 95 100 agg gat att aga tct tcg ctt cct ata ttt ctt tgt tca ctt ttg caa 3032 Arg Asp Ile Arg Ser Ser Leu Pro Ile Phe Leu Cys Ser Leu Leu Gln 105 110 115 ggt cta tat cta taa tcg cat act ttt agt ttc ata gtg aaa tgg ctg 3080 Gly Leu Tyr Leu * Ser His Thr Phe Ser Phe Ile Val Lys Trp Leu 120 125 130 tgc cat ttt cat aca atc tat aaa aac tgt ttt tta tat aca ggc gaa 3128 Cys His Phe His Thr Ile Tyr Lys Asn Cys Phe Leu Tyr Thr Gly Glu 135 140 145 agg gcc tct agc tga att ggt tag gtg gtc tga gta gca ctt ctc agg 3176 Arg Ala Ser Ser * Ile Gly * Val Val * Val Ala Leu Leu Arg 150 155 160 tcc tgg

ggt cga ctc ccc gtg gga gcg aat ttt agg ctg tgg tta aaa 3224 Ser Trp Gly Arg Leu Pro Val Gly Ala Asn Phe Arg Leu Trp Leu Lys 165 170 175 aaa atc ctc tcg tct gtc cca cgt caa agc ata ggt cta agg ctc ggc 3272 Lys Ile Leu Ser Ser Val Pro Arg Gln Ser Ile Gly Leu Arg Leu Gly 180 185 190 ccc ggt cgc ggt cgt tct gac atg ggc ttc gat gcc gct gtg tat ggg 3320 Pro Gly Arg Gly Arg Ser Asp Met Gly Phe Asp Ala Ala Val Tyr Gly 195 200 205 tgg ggc agg ggt ttg ggg gtt ttc tcg acc tgt gta aaa agg tct tct 3368 Trp Gly Arg Gly Leu Gly Val Phe Ser Thr Cys Val Lys Arg Ser Ser 210 215 220 tct taa tac aat act tgg ggg ctg tct tac ccc ccg cag gtc gag ttt 3416 Ser * Tyr Asn Thr Trp Gly Leu Ser Tyr Pro Pro Gln Val Glu Phe 225 230 235 tct tat ata cag cta tgt gag aga cct cta agt atc cat cta gtt ctt 3464 Ser Tyr Ile Gln Leu Cys Glu Arg Pro Leu Ser Ile His Leu Val Leu 240 245 250 255 gga tat gtt gtt cca caa tgc aag tca att ggg gaa att aac act tca 3512 Gly Tyr Val Val Pro Gln Cys Lys Ser Ile Gly Glu Ile Asn Thr Ser 260 265 270 ata gtt cat tta ttg caa tat ctc tgt tct ata gcc ctg ttc tat act 3560 Ile Val His Leu Leu Gln Tyr Leu Cys Ser Ile Ala Leu Phe Tyr Thr 275 280 285 cga tag agc cat aga ggt tga gtt gtc ata gta tgc atc cta ttt ggg 3608 Arg * Ser His Arg Gly * Val Val Ile Val Cys Ile Leu Phe Gly 290 295 300 aca cag gtg gta gga tgg cct gat tgt tac gcc cca tag tcc gac cag 3656 Thr Gln Val Val Gly Trp Pro Asp Cys Tyr Ala Pro * Ser Asp Gln 305 310 315 ctc gag tga act aca cat ttc cac caa ccc act tac act ttc atc ctc 3704 Leu Glu * Thr Thr His Phe His Gln Pro Thr Tyr Thr Phe Ile Leu 320 325 330 act tca tgt aaa aat ggt taa gct agt gtt agt ggc atg aac ttt aga 3752 Thr Ser Cys Lys Asn Gly * Ala Ser Val Ser Gly Met Asn Phe Arg 335 340 345 aac ctt ata tga taa tct aat cca ccc tta caa agc aat gtg ggt ata 3800 Asn Leu Ile * * Ser Asn Pro Pro Leu Gln Ser Asn Val Gly Ile 350 355 360 aac ccc agc aat ctc aca tca aca cac aac ata ggc cac cat ggt tta 3848 Asn Pro Ser Asn Leu Thr Ser Thr His Asn Ile Gly His His Gly Leu 365 370 375 aat tcg aac tgg gcc agt tgt cac act gat gtt atc aac tat ccc ttt 3896 Asn Ser Asn Trp Ala Ser Cys His Thr Asp Val Ile Asn Tyr Pro Phe 380 385 390 ggt ttt gtg act gcg tgg ttt atg tgc att gtg gac tta act tat att 3944 Gly Phe Val Thr Ala Trp Phe Met Cys Ile Val Asp Leu Thr Tyr Ile 395 400 405 gtg gac tgt gaa gtg gag ggt tag tag gtt tgg tat gag taa cac tgt 3992 Val Asp Cys Glu Val Glu Gly * * Val Trp Tyr Glu * His Cys 410 415 420 aca cca tca tac ttt ctg gtt ctg tca aac act ctg ttt cat gta taa 4040 Thr Pro Ser Tyr Phe Leu Val Leu Ser Asn Thr Leu Phe His Val * 425 430 435 act tgt tac taa cct tgg tga tta gcg agg acg atc tct cca tta gag 4088 Thr Cys Tyr * Pro Trp * Leu Ala Arg Thr Ile Ser Pro Leu Glu 440 445 450 gag ata atc ttt tac tat ata aag cgt cag ttc cta tgg ttt cac ggt 4136 Glu Ile Ile Phe Tyr Tyr Ile Lys Arg Gln Phe Leu Trp Phe His Gly 455 460 465 ttc aca gtc gtc atg tgt tag caa caa atc tct act ata taa aac acc 4184 Phe Thr Val Val Met Cys * Gln Gln Ile Ser Thr Ile * Asn Thr 470 475 480 agt ttc cat ggt tcc acg gtc gtt gtg ccg tgc gtc acc ctt ccc ctt 4232 Ser Phe His Gly Ser Thr Val Val Val Pro Cys Val Thr Leu Pro Leu 485 490 495 tct gct tta tgc aaa aat tgt gca caa atg ggg gtt tga acc ttg gtt 4280 Ser Ala Leu Cys Lys Asn Cys Ala Gln Met Gly Val * Thr Leu Val 500 505 510 gtt ggc tcc aca ttc aca ccc acc taa cca aca gaa cac aca tat ttt 4328 Val Gly Ser Thr Phe Thr Pro Thr * Pro Thr Glu His Thr Tyr Phe 515 520 525 tgt gtt tta tta aaa caa agt cta ccc ata tga tat ata gaa acc gta 4376 Cys Val Leu Leu Lys Gln Ser Leu Pro Ile * Tyr Ile Glu Thr Val 530 535 540 gca atg cac gac atc tga cta gta ata tat aga gca cct gat gaa tcg 4424 Ala Met His Asp Ile * Leu Val Ile Tyr Arg Ala Pro Asp Glu Ser 545 550 555 tat gac cac tta aca gaa ata tgg ctt tga agt ttg aac ttc tgc cgt 4472 Tyr Asp His Leu Thr Glu Ile Trp Leu * Ser Leu Asn Phe Cys Arg 560 565 570 gtt atg tgt tta cca gaa aaa gtt acc tta aat act gat gtt aaa ttt 4520 Val Met Cys Leu Pro Glu Lys Val Thr Leu Asn Thr Asp Val Lys Phe 575 580 585 agc tat tat ttt atg ctt att tat tta tat ttt att gtg acc act tgc 4568 Ser Tyr Tyr Phe Met Leu Ile Tyr Leu Tyr Phe Ile Val Thr Thr Cys 590 595 600 cct gac tat tgg gtt gtc tca ttt ctg tct caa tct gtt aac ttt ttt 4616 Pro Asp Tyr Trp Val Val Ser Phe Leu Ser Gln Ser Val Asn Phe Phe 605 610 615 cct tct gtt ttg tga cac agt tgg ata tat gca agg aaa tgg cca aca 4664 Pro Ser Val Leu * His Ser Trp Ile Tyr Ala Arg Lys Trp Pro Thr 620 625 630 tat gtt caa gga cct caa ttg agc aca ctt tgc agc ctt ttg aca ctt 4712 Tyr Val Gln Gly Pro Gln Leu Ser Thr Leu Cys Ser Leu Leu Thr Leu 635 640 645 650 tgt gca tgg ctt gtt gtc att tcc cct ata gca gtt ctg ctc gca tgg 4760 Cys Ala Trp Leu Val Val Ile Ser Pro Ile Ala Val Leu Leu Ala Trp 655 660 665 gga agc gtc ctt att gct ctt ctg gaa agg aat ata att ggt tta gct 4808 Gly Ser Val Leu Ile Ala Leu Leu Glu Arg Asn Ile Ile Gly Leu Ala 670 675 680 gtt ata atg acg ggt gtt gct ttg ctc ctg tca ttc tac tct ata atg 4856 Val Ile Met Thr Gly Val Ala Leu Leu Leu Ser Phe Tyr Ser Ile Met 685 690 695 ctc tgg tgg aga aca caa tgg caa agc tca agt atg tta tct ttg ctt 4904 Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Ser Met Leu Ser Leu Leu 700 705 710 tat ctt gat taa cct agc gtt tat ttc tct tgg caa ctg gat cct ata 4952 Tyr Leu Asp * Pro Ser Val Tyr Phe Ser Trp Gln Leu Asp Pro Ile 715 720 725 tat ata tat ata tat ata tat cta tat aca tac ata cat ata tat ata 5000 Tyr Ile Tyr Ile Tyr Ile Tyr Leu Tyr Thr Tyr Ile His Ile Tyr Ile 730 735 740 745 tag aga gag ata tcc aca ctt aat ttc tgg tca tgg ctt cat gaa taa 5048 * Arg Glu Ile Ser Thr Leu Asn Phe Trp Ser Trp Leu His Glu * 750 755 aaa ttt tga ccg aca gag gag gaa cta ggt tta tga att aat ttt ttt 5096 Lys Phe * Pro Thr Glu Glu Glu Leu Gly Leu * Ile Asn Phe Phe 760 765 770 gat caa cat acg agg agt ggc att gta aat aag aag aaa tag gca acc 5144 Asp Gln His Thr Arg Ser Gly Ile Val Asn Lys Lys Lys * Ala Thr 775 780 785 aga ttc gag cct aag aag act cgg atc tgg gtg gtg gtg ggg ttg tgc 5192 Arg Phe Glu Pro Lys Lys Thr Arg Ile Trp Val Val Val Gly Leu Cys 790 795 800 att gag act tcc cac caa cta agg tag gcc caa ttc ttg tgg ggt tca 5240 Ile Glu Thr Ser His Gln Leu Arg * Ala Gln Phe Leu Trp Gly Ser 805 810 815 tgg tct gat att ttg aaa ttt ggt gtt cac cta taa tat tca cca aga 5288 Trp Ser Asp Ile Leu Lys Phe Gly Val His Leu * Tyr Ser Pro Arg 820 825 830 aat tta tta cct cag aaa ttt gaa tta taa aga ata ctg tta caa aac 5336 Asn Leu Leu Pro Gln Lys Phe Glu Leu * Arg Ile Leu Leu Gln Asn 835 840 845 atg ttt ttc cgc atg aaa gct gtg tgc cat tat att aag aag aag tgg 5384 Met Phe Phe Arg Met Lys Ala Val Cys His Tyr Ile Lys Lys Lys Trp 850 855 860 865 aga ttt aga aac cct cta ccc tca ccc caa aat aag tta cgt atc agc 5432 Arg Phe Arg Asn Pro Leu Pro Ser Pro Gln Asn Lys Leu Arg Ile Ser 870 875 880 aca tca ttt aaa aca ttt cta aaa tgc gat tta taa atg ttt tca gta 5480 Thr Ser Phe Lys Thr Phe Leu Lys Cys Asp Leu * Met Phe Ser Val 885 890 895 gtt gtc act gtc att att gat ctt gtt gtg tta act aat gac atg ttt 5528 Val Val Thr Val Ile Ile Asp Leu Val Val Leu Thr Asn Asp Met Phe 900 905 910 att gca gag gct gtt gct tac ctt ctc ctt ctg gca gta ggc ctg cta 5576 Ile Ala Glu Ala Val Ala Tyr Leu Leu Leu Leu Ala Val Gly Leu Leu 915 920 925 tgt gcc tac gac ttt tgt gct att tat gtg aca gct ggt gct agt gct 5624 Cys Ala Tyr Asp Phe Cys Ala Ile Tyr Val Thr Ala Gly Ala Ser Ala 930 935 940 tcc gag ctt aat tct cca tca ggg ttc ttc ttc ggg gtg tct gta ata 5672 Ser Glu Leu Asn Ser Pro Ser Gly Phe Phe Phe Gly Val Ser Val Ile 945 950 955 960 tca ttg gcc atc aat atg ctt ttt ata tgt aaa ata ctg ttt aat ggt 5720 Ser Leu Ala Ile Asn Met Leu Phe Ile Cys Lys Ile Leu Phe Asn Gly 965 970 975 aaa gct tct gtt ttc tgt ttt tca ctt gtc tgt caa tat gtt taa ctc 5768 Lys Ala Ser Val Phe Cys Phe Ser Leu Val Cys Gln Tyr Val * Leu 980 985 990 atc atg att aac ttc act ttc ttg cgc att tta tgc aca gta agt gga 5816 Ile Met Ile Asn Phe Thr Phe Leu Arg Ile Leu Cys Thr Val Ser Gly 995 1000 1005 ttt gat gtt gat gaa tat gtg cgg agg tca tac aaa ttt gcc tat tct 5864 Phe Asp Val Asp Glu Tyr Val Arg Arg Ser Tyr Lys Phe Ala Tyr Ser 1010 1015 1020 gac tgt gtt gaa gtg gct cct gtt tca tgc tct cct gag cca ccg gat 5912 Asp Cys Val Glu Val Ala Pro Val Ser Cys Ser Pro Glu Pro Pro Asp 1025 1030 1035 cct agt gaa tta tac atg aca aaa tcc agc agg taa att gac ttc tat 5960 Pro Ser Glu Leu Tyr Met Thr Lys Ser Ser Arg * Ile Asp Phe Tyr 1040 1045 1050 ttt ttc cta tca tac aat ctt gta act tgt ata cct aat tat tta tca 6008 Phe Phe Leu Ser Tyr Asn Leu Val Thr Cys Ile Pro Asn Tyr Leu Ser 1055 1060 1065 1070 ata ctg ttt tac tag taa ttt ttt tga gga atg ggt ttt gcg cat tat 6056 Ile Leu Phe Tyr * * Phe Phe * Gly Met Gly Phe Ala His Tyr 1075 1080 atg tcc act ggc aaa atc agg agt gta gct tta ata aag ttt cca gct 6104 Met Ser Thr Gly Lys Ile Arg Ser Val Ala Leu Ile Lys Phe Pro Ala 1085 1090 1095 acg ctc act gga tag att tag tga ctg gaa ttt aag aat agt aga gaa 6152 Thr Leu Thr Gly * Ile * * Leu Glu Phe Lys Asn Ser Arg Glu 1100 1105 1110 ggc cca aaa ctt att att ttt tca cta ttg tgt ttt tgg tct gtg tgc 6200 Gly Pro Lys Leu Ile Ile Phe Ser Leu Leu Cys Phe Trp Ser Val Cys 1115 1120 1125 atg atg tgc ttg gat tta tat ggt tat att gtg gaa tgg tgg gag tgc 6248 Met Met Cys Leu Asp Leu Tyr Gly Tyr Ile Val Glu Trp Trp Glu Cys 1130 1135 1140 taa att cta tcc ata ctt gtg ctt cca act caa ata agt cac atg ttt 6296 * Ile Leu Ser Ile Leu Val Leu Pro Thr Gln Ile Ser His Met Phe 1145 1150 1155 ctt tgt att ata ttt ttc ttt atc att ttg aga aaa tgt tca cag ctt 6344 Leu Cys Ile Ile Phe Phe Phe Ile Ile Leu Arg Lys Cys Ser Gln Leu 1160 1165 1170 1175 cta acc tgg taa tgc agg gtc aag cat tta ggg ctt ctc tac att agc 6392 Leu Thr Trp * Cys Arg Val Lys His Leu Gly Leu Leu Tyr Ile Ser 1180 1185 1190 tct ctg ctt gtg ctt gtt ggt tat tcc atc ttg tac ggt ctt acg tca 6440 Ser Leu Leu Val Leu Val Gly Tyr Ser Ile Leu Tyr Gly Leu Thr Ser 1195 1200 1205 aaa gaa gct cgt tgg ttg ggt gct tta act tca gtt gca gtt gtt atc 6488 Lys Glu Ala Arg Trp Leu Gly Ala Leu Thr Ser Val Ala Val Val Ile 1210 1215 1220 ctt ggt aac gtt ttt tct tcc ttg cat cat tga att ttc act ctg tat 6536 Leu Gly Asn Val Phe Ser Ser Leu His His * Ile Phe Thr Leu Tyr 1225 1230 1235 ctg ttt ctt gac ctc tta ttt tgc ttt cta cag act gga atc tgg gct 6584 Leu Phe Leu Asp Leu Leu Phe Cys Phe Leu Gln Thr Gly Ile Trp Ala 1240 1245 1250 tat gtt cat tta gat ttg agc ttc tta aaa gta gga tga tag tgt tat 6632 Tyr Val His Leu Asp Leu Ser Phe Leu Lys Val Gly * * Cys Tyr 1255 1260 1265 ttg tgg ctg gaa cat caa ggg ctt tcc ttg tat cct ttg gag tgc att 6680 Leu Trp Leu Glu His Gln Gly Leu Ser Leu Tyr Pro Leu Glu Cys Ile 1270 1275 1280 act ggt ttg tat tca ata ttc atc tct ttc ttt gta ttg ata aac att 6728 Thr Gly Leu Tyr Ser Ile Phe Ile Ser Phe Phe Val Leu Ile Asn Ile 1285 1290 1295 tgg gac cta gta ttc ttc tgc ctc ttt gct aac tag ctg tta cat tag 6776 Trp Asp Leu Val Phe Phe Cys Leu Phe Ala Asn * Leu Leu His * 1300 1305 1310 gta cct tgg cca ttg cat cag cta tgc ttt tgt agc atc tgt gct ttt 6824 Val Pro Trp Pro Leu His Gln Leu Cys Phe Cys Ser Ile Cys Ala Phe 1315 1320 1325 atc tgc tgc tgt ttc ttc ctg gct ttc tat ttc aaa ccc ctc agt tgc 6872 Ile Cys Cys Cys Phe Phe Leu Ala Phe Tyr Phe Lys Pro Leu Ser Cys 1330 1335 1340 1345 aag gat aga cgc cct aag aag tac agt aat aaa gct acg aga ggg att 6920 Lys Asp Arg Arg Pro Lys Lys Tyr Ser Asn Lys Ala Thr Arg Gly Ile 1350 1355 1360 tcg aag aaa agg aca aaa tag ttc ttc aaa ttc atc aga agg ctg tgg 6968 Ser Lys Lys Arg Thr Lys * Phe Phe Lys Phe Ile Arg Arg Leu Trp 1365 1370 1375 ctc tag tgt gaa gcg tag tag cgg tag tgt tga agc tgg tca aaa tgg 7016 Leu * Cys Glu Ala * * Arg * Cys * Ser Trp Ser Lys Trp 1380 1385 taa tgc aac gga ttc tat gta cag aag caa ctc aca aag cga tgg tgt 7064 * Cys Asn Gly Phe Tyr Val Gln Lys Gln Leu Thr Lys Arg Trp Cys 1390 1395 1400 caa ttg gag cag tat tcc ttt tga tcg atc aaa cag ttg tca aga agg 7112 Gln Leu Glu Gln Tyr Ser Phe * Ser Ile Lys Gln Leu Ser Arg Arg 1405 1410 1415 ccg gag ctc tga caa gaa cat aga tag tgc acg tgc aag ctt agc tca 7160 Pro Glu Leu * Gln Glu His Arg * Cys Thr Cys Lys Leu Ser Ser 1420 1425 1430 tcg gag taa ttc atg ctt atc tgc tgt cca aga ctc tga aac cgc tgt 7208 Ser Glu * Phe Met Leu Ile Cys Cys Pro Arg Leu * Asn Arg Cys 1435 1440 1445 tgt ttc agt aga tag gca tgg aga tcc cac tac ttc act tgt ttg ttc 7256 Cys Phe Ser Arg * Ala Trp Arg Ser His Tyr Phe Thr Cys Leu Phe 1450 1455 1460 tag cag tgg ttt gga aag tca tgg ctg tga gcc tag tgg atc agc cac 7304 * Gln Trp Phe Gly Lys Ser Trp Leu * Ala * Trp Ile Ser His 1465 1470 cac ctc agg taa tca aca gct att gga ttt gaa cct ggc agc aat att 7352 His Leu Arg * Ser Thr Ala Ile Gly Phe Glu Pro Gly Ser Asn Ile 1475 1480 1485 tca gga cag att aaa tga tcc aag gat ttc atc tat gct aaa aaa gaa 7400 Ser Gly Gln Ile Lys * Ser Lys Asp Phe Ile Tyr Ala Lys Lys Glu 1490 1495 1500 cgg tgg act tgg aga tgt aga act ggc taa tct tct tca gga taa agg 7448 Arg Trp Thr Trp Arg Cys Arg Thr Gly * Ser Ser Ser Gly * Arg 1505 1510 1515 act aga tcc aaa ttt ttc ata cat gct gaa aga caa agt tat gga tcc 7496 Thr Arg Ser Lys Phe Phe Ile His Ala Glu Arg Gln Ser Tyr Gly Ser 1520 1525 1530 acg tat ttt ggc ttt gct aca gag gag cag ctt gga tgc aga tag aga 7544 Thr Tyr Phe Gly Phe Ala Thr Glu Glu Gln Leu Gly Cys Arg * Arg 1535 1540 1545 gca tca aga tga cgt aga tgt cac agc tac tga ttc aga tag att gga 7592 Ala Ser Arg * Arg Arg Cys His Ser Tyr * Phe Arg * Ile Gly 1550 1555 1560 tac cac tat tgc aaa tca gat ttc tct gtc aga aga act aag gag aag 7640 Tyr His Tyr Cys Lys Ser Asp Phe Ser Val Arg Arg Thr Lys Glu Lys 1565 1570 1575 tgg tct aga aaa atg gtt gaa cat ttc aag gct aat att cca tca ttt 7688 Trp Ser Arg Lys Met Val Glu His Phe Lys Ala Asn Ile Pro Ser Phe 1580 1585 1590 agc tgg atc tcc aat acg tgc ttt tat tgt ttt cac agt aat gtt tat 7736 Ser Trp Ile Ser Asn Thr Cys Phe Tyr Cys Phe His Ser Asn Val Tyr 1595 1600 1605 aat aga gac tgc tac tgt ggc tat cta

tcg acc aga gac cat caa ggt 7784 Asn Arg Asp Cys Tyr Cys Gly Tyr Leu Ser Thr Arg Asp His Gln Gly 1610 1615 1620 1625 gat aaa tgc aac aca tga aca ggt aaa ttg ttg cag tac aat gta att 7832 Asp Lys Cys Asn Thr * Thr Gly Lys Leu Leu Gln Tyr Asn Val Ile 1630 1635 1640 cct ttg aaa gtt tgg cta tta gtt tga cca ata gag att ttc tta att 7880 Pro Leu Lys Val Trp Leu Leu Val * Pro Ile Glu Ile Phe Leu Ile 1645 1650 1655 tgg cag ttt gaa ttt ggt ttc tcg ata ctg ctt ctg tca cca gtt gtc 7928 Trp Gln Phe Glu Phe Gly Phe Ser Ile Leu Leu Leu Ser Pro Val Val 1660 1665 1670 tgc tcc att atg gca ttc att tgg tct ctg cgt gct gaa gaa atg ttg 7976 Cys Ser Ile Met Ala Phe Ile Trp Ser Leu Arg Ala Glu Glu Met Leu 1675 1680 1685 atg aca tcc aag ccc cag aag gtg atg cac taa aat aaa aat agt ttc 8024 Met Thr Ser Lys Pro Gln Lys Val Met His * Asn Lys Asn Ser Phe 1690 1695 1700 act att tgg cat acc tgg ata tga ttg ttt tat taa tat gac aac ttt 8072 Thr Ile Trp His Thr Trp Ile * Leu Phe Tyr * Tyr Asp Asn Phe 1705 1710 1715 tta ccc ata tcg gaa cta gtg cct cat act gtt ttt ggt tga aca ttt 8120 Leu Pro Ile Ser Glu Leu Val Pro His Thr Val Phe Gly * Thr Phe 1720 1725 1730 act atg act agc ttc tgt gta gtg taa cat gaa tat agt taa tga gag 8168 Thr Met Thr Ser Phe Cys Val Val * His Glu Tyr Ser * * Glu 1735 1740 aaa aaa atc atg tct caa atg tag taa ttc ctt acc agt ctg cac ata 8216 Lys Lys Ile Met Ser Gln Met * * Phe Leu Thr Ser Leu His Ile 1745 1750 1755 taa tcg gtt tgt agt aga aaa ata ttt ttt ttt tac ctg gga gct agg 8264 * Ser Val Cys Ser Arg Lys Ile Phe Phe Phe Tyr Leu Gly Ala Arg 1760 1765 1770 tgc cta ggt atg ctg aaa cat cta atc ctg tca aca aaa att tct tat 8312 Cys Leu Gly Met Leu Lys His Leu Ile Leu Ser Thr Lys Ile Ser Tyr 1775 1780 1785 att atg aca tgt gct tta tct gcc att aga tac aat gcc gta tta gtt 8360 Ile Met Thr Cys Ala Leu Ser Ala Ile Arg Tyr Asn Ala Val Leu Val 1790 1795 1800 1805 gct att gtt tta ata tta ctg gtc tca act ata aca act tgc ctt tct 8408 Ala Ile Val Leu Ile Leu Leu Val Ser Thr Ile Thr Thr Cys Leu Ser 1810 1815 1820 ttc agt atg gtt tca ttg cat ggc tac tga gca cat gtg ttg gtt tgt 8456 Phe Ser Met Val Ser Leu His Gly Tyr * Ala His Val Leu Val Cys 1825 1830 1835 ttc tct ctt tct taa ggt aca cat act tct ctt gca tct gaa aca tat 8504 Phe Ser Leu Ser * Gly Thr His Thr Ser Leu Ala Ser Glu Thr Tyr 1840 1845 1850 tta ctg tta tag ttt tag ttg ata cta att aac ctt ttg tgc ctt agt 8552 Leu Leu Leu * Phe * Leu Ile Leu Ile Asn Leu Leu Cys Leu Ser 1855 1860 1865 tgg tta tct att tac aca aag aca taa agt ttt ata tgc ata agc aat 8600 Trp Leu Ser Ile Tyr Thr Lys Thr * Ser Phe Ile Cys Ile Ser Asn 1870 1875 1880 aca ttg ttt caa tat act ccc tcc agt ttg caa ata gct gaa gtt ttt 8648 Thr Leu Phe Gln Tyr Thr Pro Ser Ser Leu Gln Ile Ala Glu Val Phe 1885 1890 1895 ttt tac gac aac atg gtc aac ata gct ttg acc act act ttg tat tag 8696 Phe Tyr Asp Asn Met Val Asn Ile Ala Leu Thr Thr Thr Leu Tyr * 1900 1905 1910 agt ata aat gaa tat ctt aac taa ggg atg tgt gtg tgc ctg tgt ggg 8744 Ser Ile Asn Glu Tyr Leu Asn * Gly Met Cys Val Cys Leu Cys Gly 1915 1920 1925 tgt gca cgt gca tgt ggg tgt ctg ttg taa ggg ctc tta acc tac ata 8792 Cys Ala Arg Ala Cys Gly Cys Leu Leu * Gly Leu Leu Thr Tyr Ile 1930 1935 1940 gtt ctc ctg cat gtt caa gaa aaa aga aag tac ttt tca aaa aaa tgt 8840 Val Leu Leu His Val Gln Glu Lys Arg Lys Tyr Phe Ser Lys Lys Cys 1945 1950 1955 atg tat aat tgt atg tga tct tca gtt ttc tta act aaa tat ctt tta 8888 Met Tyr Asn Cys Met * Ser Ser Val Phe Leu Thr Lys Tyr Leu Leu 1960 1965 1970 aaa gtt att tgt aat gat ttt ttt ttt aaa gtt tga ctc aaa cct tgt 8936 Lys Val Ile Cys Asn Asp Phe Phe Phe Lys Val * Leu Lys Pro Cys 1975 1980 1985 cca aag cgg caa gta ttt gca aac tgg tta act gga tgc ggc att cgt 8984 Pro Lys Arg Gln Val Phe Ala Asn Trp Leu Thr Gly Cys Gly Ile Arg 1990 1995 2000 ttt att tta ttt tgt ctt tta tgt cat aac ata ttg tca ttt tta gta 9032 Phe Ile Leu Phe Cys Leu Leu Cys His Asn Ile Leu Ser Phe Leu Val 2005 2010 2015 ttg tac tgt tag ttc att tat gca atg ctt tct tag ttt ttg cac aac 9080 Leu Tyr Cys * Phe Ile Tyr Ala Met Leu Ser * Phe Leu His Asn 2020 2025 2030 aaa tcg tgt gac tta tta tgt gtc tag agt ggg cca atg cag tta gtc 9128 Lys Ser Cys Asp Leu Leu Cys Val * Ser Gly Pro Met Gln Leu Val 2035 2040 2045 aaa gca tga aga tgt gtt ttt aga gca taa agg taa gct gcg cca aaa 9176 Lys Ala * Arg Cys Val Phe Arg Ala * Arg * Ala Ala Pro Lys 2050 2055 2060 tag aca tga aaa gtg tca ttg agg caa gaa aac ata atg gat tta cca 9224 * Thr * Lys Val Ser Leu Arg Gln Glu Asn Ile Met Asp Leu Pro 2065 2070 2075 att tag ata ttt tag cac ata caa gta agc ata ttc taa ctc agc act 9272 Ile * Ile Phe * His Ile Gln Val Ser Ile Phe * Leu Ser Thr 2080 2085 aga aac aga aaa tgt gaa tat ata cca ttc gtt aat tgg gaa cag aag 9320 Arg Asn Arg Lys Cys Glu Tyr Ile Pro Phe Val Asn Trp Glu Gln Lys 2090 2095 2100 gga cct gtc aag ggc gtg gta gtt caa ggt agc tgg acc tcg gct aag 9368 Gly Pro Val Lys Gly Val Val Val Gln Gly Ser Trp Thr Ser Ala Lys 2105 2110 2115 2120 gga cct gcc aag ggc gtt caa ggt agc tgg cct tga cta agg gac ctg 9416 Gly Pro Ala Lys Gly Val Gln Gly Ser Trp Pro * Leu Arg Asp Leu 2125 2130 2135 tca agg gtg ggc cgg ggg ggg tca agg tag ctg gac ctc ggc tac ata 9464 Ser Arg Val Gly Arg Gly Gly Ser Arg * Leu Asp Leu Gly Tyr Ile 2140 2145 2150 gtt cat agt ttt ggt ttg taa tgg tgt ggg gtt gtt ttc cta gac acc 9512 Val His Ser Phe Gly Leu * Trp Cys Gly Val Val Phe Leu Asp Thr 2155 2160 2165 cta tgg tga ggg ggt gaa att aag aga gat ggg gaa gat tgg aaa aca 9560 Leu Trp * Gly Gly Glu Ile Lys Arg Asp Gly Glu Asp Trp Lys Thr 2170 2175 2180 ata tgt tat tgc tta atg ttt gat cac cac cct tag aga taa ata ggc 9608 Ile Cys Tyr Cys Leu Met Phe Asp His His Pro * Arg * Ile Gly 2185 2190 tat tgg cct cta ata acc tga ggt gga aac tcc tag aat taa ggc cag 9656 Tyr Trp Pro Leu Ile Thr * Gly Gly Asn Ser * Asn * Gly Gln 2195 2200 2205 aat tgg cca tgc aaa caa cat ggt cac ttt cct ctt gtc caa cgt ggt 9704 Asn Trp Pro Cys Lys Gln His Gly His Phe Pro Leu Val Gln Arg Gly 2210 2215 2220 gat cac gct cgt cat ggt gcg gcg cgc ggt gat tgc ggc cct tgt ggt 9752 Asp His Ala Arg His Gly Ala Ala Arg Gly Asp Cys Gly Pro Cys Gly 2225 2230 2235 gtc act agt gca ggc ttg gtg aga gta gtg ggc ccc aca aga cat ctc 9800 Val Thr Ser Ala Gly Leu Val Arg Val Val Gly Pro Thr Arg His Leu 2240 2245 2250 2255 tcc tct aca agc agc tct ttc tcg agc taa aat gaa gga tgg cac tcg 9848 Ser Ser Thr Ser Ser Ser Phe Ser Ser * Asn Glu Gly Trp His Ser 2260 2265 2270 atg aag ttg tcg atg tct tcc cat att gtg gat aac aca agc tcg cct 9896 Met Lys Leu Ser Met Ser Ser His Ile Val Asp Asn Thr Ser Ser Pro 2275 2280 2285 ttc cac tgt att aag acc cgg tgg aca tca tgg aca agg tga gtg tgt 9944 Phe His Cys Ile Lys Thr Arg Trp Thr Ser Trp Thr Arg * Val Cys 2290 2295 2300 acg acg tgt ttt ggt tac cgg gca gcg gca ccg ttg tga atg atc gac 9992 Thr Thr Cys Phe Gly Tyr Arg Ala Ala Ala Pro Leu * Met Ile Asp 2305 2310 2315 aat gga ggg ggt gtt tgg tgg cct gca gac aga cta ttg aga agg cca 10040 Asn Gly Gly Gly Val Trp Trp Pro Ala Asp Arg Leu Leu Arg Arg Pro 2320 2325 2330 acg tgg aaa aca tca tgg agc cgt gcc tgg tgc gga aga gct agt cga 10088 Thr Trp Lys Thr Ser Trp Ser Arg Ala Trp Cys Gly Arg Ala Ser Arg 2335 2340 2345 atg gcg aca tcg ttg atg agt tcg gtg atg tgg ttt gcc ctg tag aag 10136 Met Ala Thr Ser Leu Met Ser Ser Val Met Trp Phe Ala Leu * Lys 2350 2355 2360 cgt ggc ttg agc ttt cct ttg gtc gtg gct ggc agg agg ctg gag ctc 10184 Arg Gly Leu Ser Phe Pro Leu Val Val Ala Gly Arg Arg Leu Glu Leu 2365 2370 2375 gga gac gga gtc aca acc aca ccc aat cac cca caa cgt agg ata ttg 10232 Gly Asp Gly Val Thr Thr Thr Pro Asn His Pro Gln Arg Arg Ile Leu 2380 2385 2390 2395 cac aat gga gct tgt cat agt ggt gct ttt gga cgg cct gcg cct gct 10280 His Asn Gly Ala Cys His Ser Gly Ala Phe Gly Arg Pro Ala Pro Ala 2400 2405 2410 cta ggt ggt agc gga cat tcg cta gaa att cat ttc tat ttg cca tgt 10328 Leu Gly Gly Ser Gly His Ser Leu Glu Ile His Phe Tyr Leu Pro Cys 2415 2420 2425 tct tgg cca tcg cca caa cct gtc tcg ttg ggt tcg tag gag tgg atg 10376 Ser Trp Pro Ser Pro Gln Pro Val Ser Leu Gly Ser * Glu Trp Met 2430 2435 2440 atg ggg tag ttg tcg cgg tct tag acc gcc tta aat gta ttg tct aga 10424 Met Gly * Leu Ser Arg Ser * Thr Ala Leu Asn Val Leu Ser Arg 2445 2450 2455 ggg aga aac tgt atg tgt tct ttt aga tgt act cgg ccc agg gta gcc 10472 Gly Arg Asn Cys Met Cys Ser Phe Arg Cys Thr Arg Pro Arg Val Ala 2460 2465 2470 ggc tag cca ccg atg ggg acg ata gcc aga gaa gcg gca cag gta cat 10520 Gly * Pro Pro Met Gly Thr Ile Ala Arg Glu Ala Ala Gln Val His 2475 2480 2485 gac gat gac acg gtt gtt cgt ctc tgt atg agc att gga act tag ttg 10568 Asp Asp Asp Thr Val Val Arg Leu Cys Met Ser Ile Gly Thr * Leu 2490 2495 2500 tct tgt gta gtt tgg cgc caa tcg gcc cat gag gcg cat gag ctc cca 10616 Ser Cys Val Val Trp Arg Gln Ser Ala His Glu Ala His Glu Leu Pro 2505 2510 2515 cta gga tgt gga ggt gaa gat ggg gtc aag gtt gga gac cat caa ttg 10664 Leu Gly Cys Gly Gly Glu Asp Gly Val Lys Val Gly Asp His Gln Leu 2520 2525 2530 ggg cat gcc atg cag gcg gac cat gtt ggt gaa gaa agc cga cgc cac 10712 Gly His Ala Met Gln Ala Asp His Val Gly Glu Glu Ser Arg Arg His 2535 2540 2545 2550 aga ctg aca tgt agg ggt gag cca aca aca tga agt ggt tgt agt tac 10760 Arg Leu Thr Cys Arg Gly Glu Pro Thr Thr * Ser Gly Cys Ser Tyr 2555 2560 2565 tgc aac ggt tga cca gag gat cac aag ctc gct ctt gat gcg agg caa 10808 Cys Asn Gly * Pro Glu Asp His Lys Leu Ala Leu Asp Ala Arg Gln 2570 2575 2580 ggc cta gat gaa gtc tag ctc gat gtc aga cca tac caa caa agg gag 10856 Gly Leu Asp Glu Val * Leu Asp Val Arg Pro Tyr Gln Gln Arg Glu 2585 2590 2595 ggg cac gag tag tcc aac caa atg aaa atg ttc tga ctt gta gcg ctg 10904 Gly His Glu * Ser Asn Gln Met Lys Met Phe * Leu Val Ala Leu 2600 2605 aca agt agc tcg ccc ctg gtg cat tta gtg gtg ggt gcg ctg aag ccc 10952 Thr Ser Ser Ser Pro Leu Val His Leu Val Val Gly Ala Leu Lys Pro 2610 2615 2620 2625 tcg tgg ctg tcg tca tgg aca ata gcc acc aac cct gcg agt agg gga 11000 Ser Trp Leu Ser Ser Trp Thr Ile Ala Thr Asn Pro Ala Ser Arg Gly 2630 2635 2640 gac gtt ggg acg att aga ggt gtg agt tgt agg cca cca tgc tgt cga 11048 Asp Val Gly Thr Ile Arg Gly Val Ser Cys Arg Pro Pro Cys Cys Arg 2645 2650 2655 tga tgg atc agg gtg tga caa gct ggc ttg cga caa ttt tgg cgt aga 11096 * Trp Ile Arg Val * Gln Ala Gly Leu Arg Gln Phe Trp Arg Arg 2660 2665 2670 tgg cta cca agg cta ggt tcg tgg ctt aca cct ggc gga ggc ggt cga 11144 Trp Leu Pro Arg Leu Gly Ser Trp Leu Thr Pro Gly Gly Gly Gly Arg 2675 2680 2685 tga agt caa atc agg ggc gaa aga tgg tga gga tgg cac tgt cgt cgg 11192 * Ser Gln Ile Arg Gly Glu Arg Trp * Gly Trp His Cys Arg Arg 2690 2695 2700 tgt tgc gcc tag aga aaa agc tga cga taa agt tca tgg tgc cgg gtt 11240 Cys Cys Ala * Arg Lys Ser * Arg * Ser Ser Trp Cys Arg Val 2705 2710 tgt act cga tgg tga aat tga agc cca aca act tgc cca ccc aat gat 11288 Cys Thr Arg Trp * Asn * Ser Pro Thr Thr Cys Pro Pro Asn Asp 2715 2720 2725 gct ctg aaa acg tgg caa gac att ggt cta gga gaa att tca aac tgc 11336 Ala Leu Lys Thr Trp Gln Asp Ile Gly Leu Gly Glu Ile Ser Asn Cys 2730 2735 2740 aag ttc tca cta gga agc gat gac acc gaa gat agg gat gcc aat ggc 11384 Lys Phe Ser Leu Gly Ser Asp Asp Thr Glu Asp Arg Asp Ala Asn Gly 2745 2750 2755 2760 gta tgg cat gca tga ggt cga tta att ctc gct tgt acg cgt cga ggg 11432 Val Trp His Ala * Gly Arg Leu Ile Leu Ala Cys Thr Arg Arg Gly 2765 2770 2775 agc ggt ggg ata tgg cga cat gcc taa tga gga agg tga caa ggt gct 11480 Ser Gly Gly Ile Trp Arg His Ala * * Gly Arg * Gln Gly Ala 2780 2785 taa ttg gat gat cac gac cca aac ccg ttt gaa gag gcg cca cac tct 11528 * Leu Asp Asp His Asp Pro Asn Pro Phe Glu Glu Ala Pro His Ser 2790 2795 2800 cgg aga ggg cta gtg ttg ggg ctg tcg taa tca ccg tct tca ggg cct 11576 Arg Arg Gly Leu Val Leu Gly Leu Ser * Ser Pro Ser Ser Gly Pro 2805 2810 2815 aga agg cca acc aca gga agg cgt cct tgt gaa gga gca tgg tga ggg 11624 Arg Arg Pro Thr Thr Gly Arg Arg Pro Cys Glu Gly Ala Trp * Gly 2820 2825 2830 gtg tcg tga ggt ggc tga tgc att gca cag gtc gaa cga cat gat gag 11672 Val Ser * Gly Gly * Cys Ile Ala Gln Val Glu Arg His Asp Glu 2835 2840 2845 gaa ctc aga gaa cct gtt gtg tgt cca gaa cga tgt ttt gtc gac aat 11720 Glu Leu Arg Glu Pro Val Val Cys Pro Glu Arg Cys Phe Val Asp Asn 2850 2855 2860 tgc tct tga tgc gga gat cga gct tgt tga aga act tgg cgt tgt aga 11768 Cys Ser * Cys Gly Asp Arg Ala Cys * Arg Thr Trp Arg Cys Arg 2865 2870 2875 gct agg agt ttg tcg acg acg ggt ttg ggg aag gca tcc ttc acg aca 11816 Ala Arg Ser Leu Ser Thr Thr Gly Leu Gly Lys Ala Ser Phe Thr Thr 2880 2885 2890 atg gtg ttg agg gcc tca tag ttg aca tag aat tgc caa gag tcg tcg 11864 Met Val Leu Arg Ala Ser * Leu Thr * Asn Cys Gln Glu Ser Ser 2895 2900 2905 gcc ttc ctg acc agg agc acc ggt gag gag aag gcg cat gag ccg tgg 11912 Ala Phe Leu Thr Arg Ser Thr Gly Glu Glu Lys Ala His Glu Pro Trp 2910 2915 2920 tgg atc agg ccc tag gct agc atg gtg gtg cac tgg cac tcc aac tca 11960 Trp Ile Arg Pro * Ala Ser Met Val Val His Trp His Ser Asn Ser 2925 2930 2935 ttc ttg tgg gtc gcc gga tag cag cgg tag ggg cag aca acc act ggt 12008 Phe Leu Trp Val Ala Gly * Gln Arg * Gly Gln Thr Thr Thr Gly 2940 2945 2950 tgc gcc ctc aga cca tgg tga tgt ggt gct cat gag tgt gtg gtg gag 12056 Cys Ala Leu Arg Pro Trp * Cys Gly Ala His Glu Cys Val Val Glu 2955 2960 2965 tcc cta ggg ctc gat gaa gac gaa gca act cgg cga gta ggg cct cca 12104 Ser Leu Gly Leu Asp Glu Asp Glu Ala Thr Arg Arg Val Gly Pro Pro 2970 2975 2980 tgt ggt tgt tgc tcg tgc agg tct gca aag ctg cgt tgg gcg gtc cag 12152 Cys Gly Cys Cys Ser Cys Arg Ser Ala Lys Leu Arg Trp Ala Val Gln 2985 2990 2995 cca cgc cat gcc agt tca cat gat ggt cat ggt gcc aga atg aca ccg 12200 Pro Arg His Ala Ser Ser His Asp Gly His Gly Ala Arg Met Thr Pro 3000 3005 3010 3015 tga tag cgc caa aat ccc ata gga tgg gcg cga gtg aca tga gcc att 12248 * * Arg Gln Asn Pro Ile Gly Trp Ala Arg Val Thr * Ala Ile 3020 3025 ggg tcc cca aca tga tgt cgc aac cta cca gca aat agg tca ctt gaa

12296 Gly Ser Pro Thr * Cys Arg Asn Leu Pro Ala Asn Arg Ser Leu Glu 3030 3035 3040 aag ctt tgt cgt cga tgt aca aag gcg tcc cac gga aca cgc cta gat 12344 Lys Leu Cys Arg Arg Cys Thr Lys Ala Ser His Gly Thr Arg Leu Asp 3045 3050 3055 agg gaa cac act cac cat ttg cca tga tta cct tca tgt tgt cat ggt 12392 Arg Glu His Thr His His Leu Pro * Leu Pro Ser Cys Cys His Gly 3060 3065 3070 tct gag cct cct tgg ctt tga aat tgt atg tgt gtt tat ttg gta aac 12440 Ser Glu Pro Pro Trp Leu * Asn Cys Met Cys Val Tyr Leu Val Asn 3075 3080 3085 ata gag gtc tag ttt tgt taa aaa aaa tta aat atc tct ttc tga att 12488 Ile Glu Val * Phe Cys * Lys Lys Leu Asn Ile Ser Phe * Ile 3090 3095 3100 att aaa aaa tac tct ttg tat atc tag cga ata tct tta tga ctt tgg 12536 Ile Lys Lys Tyr Ser Leu Tyr Ile * Arg Ile Ser Leu * Leu Trp 3105 3110 3115 aag ctt tat gtc tgc aat caa tta atg cat ttt att ggt gaa aca tat 12584 Lys Leu Tyr Val Cys Asn Gln Leu Met His Phe Ile Gly Glu Thr Tyr 3120 3125 3130 ctg cat ttt ggt cta aaa gtc atc ttt ttt tgc tct gca gca aat cat 12632 Leu His Phe Gly Leu Lys Val Ile Phe Phe Cys Ser Ala Ala Asn His 3135 3140 3145 cag tta tat tgg gcc tgt ctc tca cgg tac cac tta tgg tgg ctt gcc 12680 Gln Leu Tyr Trp Ala Cys Leu Ser Arg Tyr His Leu Trp Trp Leu Ala 3150 3155 3160 tct cat ttg ctg ttc cca tat gga tac gca atg gtt aca gtt tct gga 12728 Ser His Leu Leu Phe Pro Tyr Gly Tyr Ala Met Val Thr Val Ser Gly 3165 3170 3175 3180 ttc ctg gaa ggg agt ttg caa atc gtg aaa atg tta gtc aag ctc cag 12776 Phe Leu Glu Gly Ser Leu Gln Ile Val Lys Met Leu Val Lys Leu Gln 3185 3190 3195 gag aga aag agg ttt gtt gtt cgg ttc ttt ata aga ttg caa cta cca 12824 Glu Arg Lys Arg Phe Val Val Arg Phe Phe Ile Arg Leu Gln Leu Pro 3200 3205 3210 act agg gat ttt ctg gtc aca tgc ata agt gta acg ttg atc tat tca 12872 Thr Arg Asp Phe Leu Val Thr Cys Ile Ser Val Thr Leu Ile Tyr Ser 3215 3220 3225 atc tct ttg ttt agt cag aag gag ata ctg aat tgc gag ttt gcg acc 12920 Ile Ser Leu Phe Ser Gln Lys Glu Ile Leu Asn Cys Glu Phe Ala Thr 3230 3235 3240 cag tct aat gtt gaa aac tgg att tac ctg aat atg tct att tca tgt 12968 Gln Ser Asn Val Glu Asn Trp Ile Tyr Leu Asn Met Ser Ile Ser Cys 3245 3250 3255 3260 caa tct ttt cca tat gtg ctc gtc ata ttt gca tgg cca tgt cat gtt 13016 Gln Ser Phe Pro Tyr Val Leu Val Ile Phe Ala Trp Pro Cys His Val 3265 3270 3275 ttt agc aca tca aat aaa atg cct aat ttg tag cac aga ttt tac taa 13064 Phe Ser Thr Ser Asn Lys Met Pro Asn Leu * His Arg Phe Tyr * 3280 3285 3290 gat tat tgt aga taa ttt aga atc gaa cta tcc aat att ggc aat taa 13112 Asp Tyr Cys Arg * Phe Arg Ile Glu Leu Ser Asn Ile Gly Asn * 3295 3300 gaa aag act tat tag tgt tgc tta ccg ttt tct ttt tct ggt ttc agc 13160 Glu Lys Thr Tyr * Cys Cys Leu Pro Phe Ser Phe Ser Gly Phe Ser 3305 3310 3315 ggg ctc tct ttg tta tca cca ttg ctg ttt tca ctg cat caa tta ttg 13208 Gly Leu Ser Leu Leu Ser Pro Leu Leu Phe Ser Leu His Gln Leu Leu 3320 3325 3330 3335 gcc ttg gtg caa tag tgt cag caa agc ctt tag acg ctc tag gct ata 13256 Ala Leu Val Gln * Cys Gln Gln Ser Leu * Thr Leu * Ala Ile 3340 3345 aag ggt ggg atg ctg ata aga aca gct cct att ctc cct atg caa cat 13304 Lys Gly Gly Met Leu Ile Arg Thr Ala Pro Ile Leu Pro Met Gln His 3350 3355 3360 caa tgt atc ttg gat ggg cat tgt ctt caa caa ttg ctg tga tta cca 13352 Gln Cys Ile Leu Asp Gly His Cys Leu Gln Gln Leu Leu * Leu Pro 3365 3370 3375 cag ggt tga tac cta ttg ttg ctt ggt ttg caa cat acc ggt ttt cac 13400 Gln Gly * Tyr Leu Leu Leu Leu Gly Leu Gln His Thr Gly Phe His 3380 3385 3390 ctt cat cag cta tat gtg ttg gcc tct ttg caa gta cgt tcg tct tga 13448 Leu His Gln Leu Tyr Val Leu Ala Ser Leu Gln Val Arg Ser Ser * 3395 3400 3405 tcc ttt ttt cgg aaa aaa tac ttt aaa tat tga ata gaa gag att gca 13496 Ser Phe Phe Arg Lys Lys Tyr Phe Lys Tyr * Ile Glu Glu Ile Ala 3410 3415 3420 gct gtt cta att cat gct ttt ctg cag ctg ttc ttg tgt ctt ttt gcg 13544 Ala Val Leu Ile His Ala Phe Leu Gln Leu Phe Leu Cys Leu Phe Ala 3425 3430 3435 3440 gtg cat cct act ggg gag tgg taa att cac gag agg atg gtg ctc ctc 13592 Val His Pro Thr Gly Glu Trp * Ile His Glu Arg Met Val Leu Leu 3445 3450 3455 taa agg ctg att tcc ttg cag cat tac ttc cct tgc ttt gca ttc cag 13640 * Arg Leu Ile Ser Leu Gln His Tyr Phe Pro Cys Phe Ala Phe Gln 3460 3465 3470 cat ttt tct cac tgt tca ctg ggc ttt aca aat ggt ata aaa agg act 13688 His Phe Ser His Cys Ser Leu Gly Phe Thr Asn Gly Ile Lys Arg Thr 3475 3480 3485 gtc ttt agc att tta ttc ccc tct gta ttt ctg atc cac tga gat caa 13736 Val Phe Ser Ile Leu Phe Pro Ser Val Phe Leu Ile His * Asp Gln 3490 3495 3500 aat tgc aaa taa ttt cag gaa gga tga tga ttg gaa gat ttc tcg tgg 13784 Asn Cys Lys * Phe Gln Glu Gly * * Leu Glu Asp Phe Ser Trp 3505 3510 cgt tta cct ttt tgt tgg cat ggg aat gtt gct gtt gtt tgg tgc ggt 13832 Arg Leu Pro Phe Cys Trp His Gly Asn Val Ala Val Val Trp Cys Gly 3515 3520 3525 3530 tgc agc tgt tat tgt cac aat cag gcc ctg gac tgt aag taa agt tta 13880 Cys Ser Cys Tyr Cys His Asn Gln Ala Leu Asp Cys Lys * Ser Leu 3535 3540 3545 tgt gac ctg gga cgt tag ttt tca att aca ata ggc tat aat tca cat 13928 Cys Asp Leu Gly Arg * Phe Ser Ile Thr Ile Gly Tyr Asn Ser His 3550 3555 3560 gtc atc ttt ata cag gtt gga gtt gct tgc ctc gta gcc att ctg ttc 13976 Val Ile Phe Ile Gln Val Gly Val Ala Cys Leu Val Ala Ile Leu Phe 3565 3570 3575 ctt gta ttt gtt att ggg gtc atc cac tac tgg aca tct aac aac ttc 14024 Leu Val Phe Val Ile Gly Val Ile His Tyr Trp Thr Ser Asn Asn Phe 3580 3585 3590 tat cta acg agg aca cag atg ctg ctt gtt tgt tcc att gct ttt ctc 14072 Tyr Leu Thr Arg Thr Gln Met Leu Leu Val Cys Ser Ile Ala Phe Leu 3595 3600 3605 tta gcc ttg gct gcc ttc ctg atg ggt tta ttt cac ggt gac tga tga 14120 Leu Ala Leu Ala Ala Phe Leu Met Gly Leu Phe His Gly Asp * * 3610 3615 3620 tct ttt ttc tat gcc cat ctg tcc ttt att tca ctt tgt tta ttg gtt 14168 Ser Phe Phe Tyr Ala His Leu Ser Phe Ile Ser Leu Cys Leu Leu Val 3625 3630 3635 tga gtt att gct atc att tag tct tgt att tac ttg gct tct gaa ctt 14216 * Val Ile Ala Ile Ile * Ser Cys Ile Tyr Leu Ala Ser Glu Leu 3640 3645 3650 tgc agg aaa gcc ttt tgt tgg agc atc tat agg tta ttt ctc att tat 14264 Cys Arg Lys Ala Phe Cys Trp Ser Ile Tyr Arg Leu Phe Leu Ile Tyr 3655 3660 3665 att tct tct cac tgg aag ggc ttt gac tgt aag ttt tgt cat gca cta 14312 Ile Ser Ser His Trp Lys Gly Phe Asp Cys Lys Phe Cys His Ala Leu 3670 3675 3680 aga taa ctg ttg ctg atc gct ttc tag ttc tca gta tac tgt aga att 14360 Arg * Leu Leu Leu Ile Ala Phe * Phe Ser Val Tyr Cys Arg Ile 3685 3690 3695 ttc ttc taa tgc tga atg gtg tgc cct tct cag gtc ctt cta tca ccg 14408 Phe Phe * Cys * Met Val Cys Pro Ser Gln Val Leu Leu Ser Pro 3700 3705 3710 cca atc gta gtg tat tcg cca aga gta ttg cct gta tac gtt tat gat 14456 Pro Ile Val Val Tyr Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp 3715 3720 3725 gct cat gca gac tct gct aaa aat gtt agg tac aag tat att ctt tct 14504 Ala His Ala Asp Ser Ala Lys Asn Val Arg Tyr Lys Tyr Ile Leu Ser 3730 3735 3740 cgt gct acc tgt ttg cct ttt ttg agt tca ggg ttg gtc ata atg tat 14552 Arg Ala Thr Cys Leu Pro Phe Leu Ser Ser Gly Leu Val Ile Met Tyr 3745 3750 3755 3760 tct tat ctc tgt tct gtc cca tcc taa aag ttc tag ttt cag ttg aga 14600 Ser Tyr Leu Cys Ser Val Pro Ser * Lys Phe * Phe Gln Leu Arg 3765 3770 aca tgc tct aat gtt gtc ttt ttt ttt aca gct atg cct ttc tta ttc 14648 Thr Cys Ser Asn Val Val Phe Phe Phe Thr Ala Met Pro Phe Leu Phe 3775 3780 3785 3790 tgt atg gga ttg cat tag caa ctg aag ttt ggg gtg tta ttg cta gtc 14696 Cys Met Gly Leu His * Gln Leu Lys Phe Gly Val Leu Leu Leu Val 3795 3800 3805 taa taa tga atc cac cat ttg ttg ggg ctg gcg ttt ctg cta cta ctc 14744 * * * Ile His His Leu Leu Gly Leu Ala Phe Leu Leu Leu Leu 3810 3815 ttg taa ttg ctt tca gtt ttg ctg ttt ctc gac cct gcc tga ctc tta 14792 Leu * Leu Leu Ser Val Leu Leu Phe Leu Asp Pro Ala * Leu Leu 3820 3825 3830 agg ttg gta att tgc tgc agc tag tac tag taa gat ttc gta ctc ctt 14840 Arg Leu Val Ile Cys Cys Ser * Tyr * * Asp Phe Val Leu Leu 3835 3840 3845 ttt tta tat gat taa tat aaa ata aat aca tat ctt ttt taa aaa tca 14888 Phe Leu Tyr Asp * Tyr Lys Ile Asn Thr Tyr Leu Phe * Lys Ser 3850 3855 atc aga tga tgg agg atg cag ttc att ttc tca gca agg ata cag ttg 14936 Ile Arg * Trp Arg Met Gln Phe Ile Phe Ser Ala Arg Ile Gln Leu 3860 3865 3870 tgc aag cga tgt cac ggt ctg cta ata aag tat gtt tta tat ttg tat 14984 Cys Lys Arg Cys His Gly Leu Leu Ile Lys Tyr Val Leu Tyr Leu Tyr 3875 3880 3885 3890 tat taa gat att caa cat aag tta ttt gtg tgc atg ctt tta tac att 15032 Tyr * Asp Ile Gln His Lys Leu Phe Val Cys Met Leu Leu Tyr Ile 3895 3900 3905 tct cca ttt tta gtt aga aac att tca acc agt ctc tag ttt gct tgc 15080 Ser Pro Phe Leu Val Arg Asn Ile Ser Thr Ser Leu * Phe Ala Cys 3910 3915 3920 tta tta gca tct cgt gct gag atc tag ttc aga act gtt cat gct taa 15128 Leu Leu Ala Ser Arg Ala Glu Ile * Phe Arg Thr Val His Ala * 3925 3930 tct gac gca tcc aag ttc tgt cat agt cct gtc att gtg tac att ggg 15176 Ser Asp Ala Ser Lys Phe Cys His Ser Pro Val Ile Val Tyr Ile Gly 3935 3940 3945 3950 atc tgt gta aat tac tat cga caa aat att tta aat cta tct cag aag 15224 Ile Cys Val Asn Tyr Tyr Arg Gln Asn Ile Leu Asn Leu Ser Gln Lys 3955 3960 3965 ctc gtc act tgt agg att gcc att gca att aga atc ctt tga aat aat 15272 Leu Val Thr Cys Arg Ile Ala Ile Ala Ile Arg Ile Leu * Asn Asn 3970 3975 3980 tca caa tgt ttg gat ctg tac atg aca gta tgt ttt tcc aga cta gaa 15320 Ser Gln Cys Leu Asp Leu Tyr Met Thr Val Cys Phe Ser Arg Leu Glu 3985 3990 3995 atg cta tat ctg gga ctt act cag cac ctc aga ggt ccg caa gtt ctg 15368 Met Leu Tyr Leu Gly Leu Thr Gln His Leu Arg Gly Pro Gln Val Leu 4000 4005 4010 ctg ctc ttt tgg ttg gag atc ctg ctc tta cat tgg aca ggg ctg gga 15416 Leu Leu Phe Trp Leu Glu Ile Leu Leu Leu His Trp Thr Gly Leu Gly 4015 4020 4025 act ttg tgc ttc cta ggg ctg atg tta tga aac tga gag atc gtt tga 15464 Thr Leu Cys Phe Leu Gly Leu Met Leu * Asn * Glu Ile Val * 4030 4035 4040 gaa atg aag aaa ttg ctg cag gat ctt tct tat gtg gag taa aag att 15512 Glu Met Lys Lys Leu Leu Gln Asp Leu Ser Tyr Val Glu * Lys Ile 4045 4050 4055 gtt tac taa ttt gcc ccc agt ccc tgt caa aca tag att atc gga gga 15560 Val Tyr * Phe Ala Pro Ser Pro Cys Gln Thr * Ile Ile Gly Gly 4060 4065 4070 ata tgt gtg ccc atg cac gta ttt tgg ctt tgg aag aag caa ttg ata 15608 Ile Cys Val Pro Met His Val Phe Trp Leu Trp Lys Lys Gln Leu Ile 4075 4080 4085 cgg aat ggg tgt ata tgt ggg aca aat ttg gtg gtt att tac ttc tgt 15656 Arg Asn Gly Cys Ile Cys Gly Thr Asn Leu Val Val Ile Tyr Phe Cys 4090 4095 4100 tgc ttg gat tga ctg cca aag ctg aac aaa tac agg tat gta ttt ggt 15704 Cys Leu Asp * Leu Pro Lys Leu Asn Lys Tyr Arg Tyr Val Phe Gly 4105 4110 4115 atg ttg tac aat ttt tta gac ccc aat ttg tta gcg att aat ggt gtg 15752 Met Leu Tyr Asn Phe Leu Asp Pro Asn Leu Leu Ala Ile Asn Gly Val 4120 4125 4130 aag cat ttc tgt att gtt tca gga tga agt tcg tct aag act ctt ttt 15800 Lys His Phe Cys Ile Val Ser Gly * Ser Ser Ser Lys Thr Leu Phe 4135 4140 4145 gga tag cat agg cct ttc cga ttt gag tgc caa aga aat taa gaa atg 15848 Gly * His Arg Pro Phe Arg Phe Glu Cys Gln Arg Asn * Glu Met 4150 4155 4160 gat gcc tga aga tcg gag gca att tga gct tat tca aga aag gta ttt 15896 Asp Ala * Arg Ser Glu Ala Ile * Ala Tyr Ser Arg Lys Val Phe 4165 4170 4175 ttt gtc tgt tac tat tag tta tac tac tga agg gca ggc cta gtg cag 15944 Phe Val Cys Tyr Tyr * Leu Tyr Tyr * Arg Ala Gly Leu Val Gln 4180 4185 4190 tgg tga gag ctg tct tac caa gtc acc agg tcg cag gtt cga agc agt 15992 Trp * Glu Leu Ser Tyr Gln Val Thr Arg Ser Gln Val Arg Ser Ser 4195 4200 4205 ccc tcc gca ttt gcg ggg gaa agg ctt gtc tca gtt tag ggg gtg ttt 16040 Pro Ser Ala Phe Ala Gly Glu Arg Leu Val Ser Val * Gly Val Phe 4210 4215 4220 ggc ttc can nnn nnn nnn nnn nnn nnn ncc cta gac ccc aac tca tgc 16088 Gly Phe Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Asp Pro Asn Ser Cys 4225 4230 4235 agg agc cta tgg cac tga gtc tgc ctc ctt tta caa tta gtt ata cca 16136 Arg Ser Leu Trp His * Val Cys Leu Leu Leu Gln Leu Val Ile Pro 4240 4245 4250 tta ttg tat gac aca taa aat aaa aat cat att gca gcc att gct att 16184 Leu Leu Tyr Asp Thr * Asn Lys Asn His Ile Ala Ala Ile Ala Ile 4255 4260 4265 cct ttg tta ttt gtg tct aca gct aca taa ggg aaa aag aaa tgg aag 16232 Pro Leu Leu Phe Val Ser Thr Ala Thr * Gly Lys Lys Lys Trp Lys 4270 4275 4280 agg agg ctt tga tgc aaa gac gag agg aag aag gga agg gaa gag aaa 16280 Arg Arg Leu * Cys Lys Asp Glu Arg Lys Lys Gly Arg Glu Glu Lys 4285 4290 4295 gga gga ggg cat tgc tag aga gag agg agc gaa aat gga agg agc tcg 16328 Gly Gly Gly His Cys * Arg Glu Arg Ser Glu Asn Gly Arg Ser Ser 4300 4305 4310 aaa tat cat tgc ttt ctt cca ttc cta ata ctg gaa gca ggg atg ctg 16376 Lys Tyr His Cys Phe Leu Pro Phe Leu Ile Leu Glu Ala Gly Met Leu 4315 4320 4325 cag cta tgg cag cag ctg tca gag ctg ttg gag gtg att ctg ccc tgg 16424 Gln Leu Trp Gln Gln Leu Ser Glu Leu Leu Glu Val Ile Leu Pro Trp 4330 4335 4340 aag att ctt ttg caa gag ata ggg tct ctt caa tag cca atc aca tac 16472 Lys Ile Leu Leu Gln Glu Ile Gly Ser Leu Gln * Pro Ile Thr Tyr 4345 4350 4355 gaa agg cac aat tgg ctc ggc gag cag aac agg tta tgt cct ctg att 16520 Glu Arg His Asn Trp Leu Gly Glu Gln Asn Arg Leu Cys Pro Leu Ile 4360 4365 4370 4375 acc ctt aat ttc atc aag tta tga aac aca cct gcc atc att tga gcg 16568 Thr Leu Asn Phe Ile Lys Leu * Asn Thr Pro Ala Ile Ile * Ala 4380 4385 ctg tag aac tgt aat tta tct ttg agt gca tgt ttt ttt ttc gaa cac 16616 Leu * Asn Cys Asn Leu Ser Leu Ser Ala Cys Phe Phe Phe Glu His 4390 4395 4400 aca gga gag ctg cgt ata att ata tta aga aga aga gga cca aag gtt 16664 Thr Gly Glu Leu Arg Ile Ile Ile Leu Arg Arg Arg Gly Pro Lys Val 4405 4410 4415 4420 aca gaa aac ccg aaa gaa ata gcc agg ttt tag gca tct aag gag cct 16712 Thr Glu Asn Pro Lys Glu Ile Ala Arg Phe * Ala Ser Lys Glu Pro 4425 4430 4435 aga acc tag cct aaa aac tac caa tct ttg agt gca tgt tag gca taa 16760 Arg Thr * Pro Lys Asn Tyr Gln Ser Leu Ser Ala Cys * Ala * 4440 4445 aca tcc ctg ggt att tgt tgt ctg ttt tgt gtc tca tct gga gct ggt 16808 Thr Ser Leu Gly Ile Cys Cys Leu Phe Cys Val Ser Ser Gly Ala Gly 4450 4455 4460 aat ctt aca aca aat aag tat tcc ctc cag tca ata

ata gtt tat gct 16856 Asn Leu Thr Thr Asn Lys Tyr Ser Leu Gln Ser Ile Ile Val Tyr Ala 4465 4470 4475 4480 atg aca ctt cag aaa aaa gca tat gga tac atg tat ctg agg gca aat 16904 Met Thr Leu Gln Lys Lys Ala Tyr Gly Tyr Met Tyr Leu Arg Ala Asn 4485 4490 4495 aag cag caa aat aac cat ttt ggc ctc taa tta att gaa gct gtg atg 16952 Lys Gln Gln Asn Asn His Phe Gly Leu * Leu Ile Glu Ala Val Met 4500 4505 4510 cac atg aac tgc aaa ggt cag gcc caa gca acg tga gta cta gtg ata 17000 His Met Asn Cys Lys Gly Gln Ala Gln Ala Thr * Val Leu Val Ile 4515 4520 4525 tta cta gtt gaa tgg agt ttc ctg gaa ttg cat taa cga ccg ttg ggg 17048 Leu Leu Val Glu Trp Ser Phe Leu Glu Leu His * Arg Pro Leu Gly 4530 4535 4540 agg taa tgg act aat ggg gtc acc cat atc ctg tcc ccg cac act atc 17096 Arg * Trp Thr Asn Gly Val Thr His Ile Leu Ser Pro His Thr Ile 4545 4550 4555 cat tcc caa att atc caa tcc aaa tat tga ggg taa tac tta tga ata 17144 His Ser Gln Ile Ile Gln Ser Lys Tyr * Gly * Tyr Leu * Ile 4560 4565 aac taa tgg gta ata gat gga gct aat ccc tgt cgc tgc ctc cac tac 17192 Asn * Trp Val Ile Asp Gly Ala Asn Pro Cys Arg Cys Leu His Tyr 4570 4575 4580 aac gct aca tcg ttg gtg cgg gct tgc tgt cca ccg cct tcc tcc agg 17240 Asn Ala Thr Ser Leu Val Arg Ala Cys Cys Pro Pro Pro Ser Ser Arg 4585 4590 4595 4600 gac tcg tca atc ggg tac acc gaa tga ggt gct gag cac cga cat ggt 17288 Asp Ser Ser Ile Gly Tyr Thr Glu * Gly Ala Glu His Arg His Gly 4605 4610 4615 atc aga gtt tga tgt gct gcc aac gtg gat gtg cca tgc cgg ggc cag 17336 Ile Arg Val * Cys Ala Ala Asn Val Asp Val Pro Cys Arg Gly Gln 4620 4625 4630 cac ccg tgc tgt agt cgc ccc gtg gtc ctg ggt tga ata tgt gga tag 17384 His Pro Cys Cys Ser Arg Pro Val Val Leu Gly * Ile Cys Gly * 4635 4640 gaa ggg gga agg gga ttc tag tag cag cag gtt gag cgc aag ccg caa 17432 Glu Gly Gly Arg Gly Phe * * Gln Gln Val Glu Arg Lys Pro Gln 4645 4650 4655 tga tgg ctc gac ttg aat ctg agg ata tgc agg ggg att cta gaa gca 17480 * Trp Leu Asp Leu Asn Leu Arg Ile Cys Arg Gly Ile Leu Glu Ala 4660 4665 4670 gca ggt tga gtg caa gct gca atg ctg gct gct gag ctg tgg cgc tgg 17528 Ala Gly * Val Gln Ala Ala Met Leu Ala Ala Glu Leu Trp Arg Trp 4675 4680 4685 tgg tgt cag gcg tga tgg cca gtg tgt gca agc cta gtg gag gcc aaa 17576 Trp Cys Gln Ala * Trp Pro Val Cys Ala Ser Leu Val Glu Ala Lys 4690 4695 4700 ttg ccc ccc tct ttg cca taa ctc ctt tat atg gtt gct act ttg cca 17624 Leu Pro Pro Ser Leu Pro * Leu Leu Tyr Met Val Ala Thr Leu Pro 4705 4710 4715 agg aat ctg cat gtc ctc atg cat gcg agc acg cct ttc cct atc cac 17672 Arg Asn Leu His Val Leu Met His Ala Ser Thr Pro Phe Pro Ile His 4720 4725 4730 att agt tgc cta ttc ctt ggc cag caa ttg tca agc ttt cat att ata 17720 Ile Ser Cys Leu Phe Leu Gly Gln Gln Leu Ser Ser Phe His Ile Ile 4735 4740 4745 4750 ctt tga tat tgt cct cat gta gcc act aaa ttt cca ctc ctt gat tct 17768 Leu * Tyr Cys Pro His Val Ala Thr Lys Phe Pro Leu Leu Asp Ser 4755 4760 4765 acc tca ccc tgc gcc act gat tgt gtg ccc tgt gca tcc gcg gtg gta 17816 Thr Ser Pro Cys Ala Thr Asp Cys Val Pro Cys Ala Ser Ala Val Val 4770 4775 4780 gcg ccc agg ggc aag aac ata ttg cga ggt gct tag ggt cgg gac agg 17864 Ala Pro Arg Gly Lys Asn Ile Leu Arg Gly Ala * Gly Arg Asp Arg 4785 4790 4795 gac aaa tag ggt cga gat caa gtc gac gta ctt atg cct ggt gag ggt 17912 Asp Lys * Gly Arg Asp Gln Val Asp Val Leu Met Pro Gly Glu Gly 4800 4805 4810 ggt gga cct aga cac cga tgg cca ctt cga cga ggg att cat ctg cct 17960 Gly Gly Pro Arg His Arg Trp Pro Leu Arg Arg Gly Ile His Leu Pro 4815 4820 4825 tac atg cca cat atg cca cac ttc ttg acc ttg act ggc atg aca cca 18008 Tyr Met Pro His Met Pro His Phe Leu Thr Leu Thr Gly Met Thr Pro 4830 4835 4840 agg gta tgt gac cac aca tgt ctt cat ggc gtg gcc att gac cac acg 18056 Arg Val Cys Asp His Thr Cys Leu His Gly Val Ala Ile Asp His Thr 4845 4850 4855 ctt gtc gga gtt ctt gag gaa ggg cgt cgg tgg atc ttt gtt gat tgg 18104 Leu Val Gly Val Leu Glu Glu Gly Arg Arg Trp Ile Phe Val Asp Trp 4860 4865 4870 4875 tca aaa gca gtt gtc aat gta ttg ttt gtt tgt ggg tag gtg gtt acc 18152 Ser Lys Ala Val Val Asn Val Leu Phe Val Cys Gly * Val Val Thr 4880 4885 4890 cgg taa tat cca cta ata tca tac ctg tac cca tga tta gat ggg taa 18200 Arg * Tyr Pro Leu Ile Ser Tyr Leu Tyr Pro * Leu Asp Gly * 4895 4900 ttg ata ccc tcc cta aac tct aca tgt att ggt ttg tgt acg tgt att 18248 Leu Ile Pro Ser Leu Asn Ser Thr Cys Ile Gly Leu Cys Thr Cys Ile 4905 4910 4915 gga tac cta gtg gca tga tca aat cac ata ttt cat tct acc tcc atc 18296 Gly Tyr Leu Val Ala * Ser Asn His Ile Phe His Ser Thr Ser Ile 4920 4925 4930 cca caa aga gta gtt gtc cta agt caa aca atc tta atc taa ttt tat 18344 Pro Gln Arg Val Val Val Leu Ser Gln Thr Ile Leu Ile * Phe Tyr 4935 4940 4945 tga gaa aag tac cta tac tta tga att atg ata cca aat aag taa tat 18392 * Glu Lys Tyr Leu Tyr Leu * Ile Met Ile Pro Asn Lys * Tyr 4950 4955 4960 taa aaa aac ctc gac ctg cgg ggg gta aga caa ccc ctg ggc att aca 18440 * Lys Asn Leu Asp Leu Arg Gly Val Arg Gln Pro Leu Gly Ile Thr 4965 4970 4975 tta aga ata aga cct cac gta ggt cga gaa aac ccc tga acc ctt gcc 18488 Leu Arg Ile Arg Pro His Val Gly Arg Glu Asn Pro * Thr Leu Ala 4980 4985 4990 cca ccc ata cac atc ggc atc gta gcc tat ggg aga acg acc acg gcc 18536 Pro Pro Ile His Ile Gly Ile Val Ala Tyr Gly Arg Thr Thr Thr Ala 4995 5000 5005 agg cgt tag gcc tat gct ttg gtg tgg gac aga cga ggg gat ttt ttt 18584 Arg Arg * Ala Tyr Ala Leu Val Trp Asp Arg Arg Gly Asp Phe Phe 5010 5015 5020 aac ctc acc cga agt ctg ctc cca tgg gga gtc aaa ctc aag aca tga 18632 Asn Leu Thr Arg Ser Leu Leu Pro Trp Gly Val Lys Leu Lys Thr * 5025 5030 5035 gga gtg cca ctc tga cca tct aac caa ctc agc tag aag aaa gac ctg 18680 Gly Val Pro Leu * Pro Ser Asn Gln Leu Ser * Lys Lys Asp Leu 5040 5045 5050 acc ttg ccc tgc tgc ttt ttt ggc cac ctt agg gtg tta ggg tca gtt 18728 Thr Leu Pro Cys Cys Phe Phe Gly His Leu Arg Val Leu Gly Ser Val 5055 5060 5065 taa gtt ttg ttg tat ctg ttc ttt gga cct tta ttc tct ctt aat ata 18776 * Val Leu Leu Tyr Leu Phe Phe Gly Pro Leu Phe Ser Leu Asn Ile 5070 5075 5080 atg atg cac aac tct cct gcg cgt ttg aga aaa aaa gtt gat acc ttt 18824 Met Met His Asn Ser Pro Ala Arg Leu Arg Lys Lys Val Asp Thr Phe 5085 5090 5095 ttt aca aat ttg ttc aaa cat agg ata att taa ctt agg aaa aaa cta 18872 Phe Thr Asn Leu Phe Lys His Arg Ile Ile * Leu Arg Lys Lys Leu 5100 5105 5110 gaa tgt cac ttt gtg gga tcg agg tag tac taa ata aga gca taa gag 18920 Glu Cys His Phe Val Gly Ser Arg * Tyr * Ile Arg Ala * Glu 5115 5120 5125 taa aaa tat ccc tac ctt aat atc tca aaa cct gtt gga atc aac ctt 18968 * Lys Tyr Pro Tyr Leu Asn Ile Ser Lys Pro Val Gly Ile Asn Leu 5130 5135 5140 gac tag agg gag tat cat gga aaa att taa acc gct gac cac ctg gaa 19016 Asp * Arg Glu Tyr His Gly Lys Ile * Thr Ala Asp His Leu Glu 5145 5150 5155 gcc ttc ttg ttt tgc ttt ttt agc aat gca ttg tac atg tat tct ttc 19064 Ala Phe Leu Phe Cys Phe Phe Ser Asn Ala Leu Tyr Met Tyr Ser Phe 5160 5165 5170 tgt ttt tag tgt ttt tct cca atg ctt gta tat cta att tgt tgt gaa 19112 Cys Phe * Cys Phe Ser Pro Met Leu Val Tyr Leu Ile Cys Cys Glu 5175 5180 5185 tgt gga aca gac tgg tat tcc agg cac tat atg cat act cga tga tga 19160 Cys Gly Thr Asp Trp Tyr Ser Arg His Tyr Met His Thr Arg * * 5190 5195 5200 acc gag gag tac tgg tcg tca ttg tgg aga act tga ctt gtg cct ctg 19208 Thr Glu Glu Tyr Trp Ser Ser Leu Trp Arg Thr * Leu Val Pro Leu 5205 5210 5215 tca aag tca aaa ggt tac ttt gtc tat tgc tgt cat ggt tca gcc tgt 19256 Ser Lys Ser Lys Gly Tyr Phe Val Tyr Cys Cys His Gly Ser Ala Cys 5220 5225 5230 atc tgg ccc agt gtg tct ttt tgg aag tga att cca aaa ggt ttg ctg 19304 Ile Trp Pro Ser Val Ser Phe Trp Lys * Ile Pro Lys Gly Leu Leu 5235 5240 5245 gga aat ctt agt ggc agg atc aga aca ggg tat gga agc tgg aca agt 19352 Gly Asn Leu Ser Gly Arg Ile Arg Thr Gly Tyr Gly Ser Trp Thr Ser 5250 5255 5260 tgg tct tcg att agt gac taa ggg tga aag gat gac tac tgt tgc taa 19400 Trp Ser Ser Ile Ser Asp * Gly * Lys Asp Asp Tyr Cys Cys * 5265 5270 5275 aga gtg gaa tat tgg tgc gtc tag tat tgc aga tgg cag gta tcc cct 19448 Arg Val Glu Tyr Trp Cys Val * Tyr Cys Arg Trp Gln Val Ser Pro 5280 5285 5290 att cac aac tat tgc att atg cat agc aaa taa aat caa agg cag cca 19496 Ile His Asn Tyr Cys Ile Met His Ser Lys * Asn Gln Arg Gln Pro 5295 5300 5305 tcc tcg ggt ttg ttg ttt tgg tac tgg cat gtg tgt tgg aac tgt gag 19544 Ser Ser Gly Leu Leu Phe Trp Tyr Trp His Val Cys Trp Asn Cys Glu 5310 5315 5320 gca gtt ggg gca tat att cta tca tga agc tct cct gct tgt tca tga 19592 Ala Val Gly Ala Tyr Ile Leu Ser * Ser Ser Pro Ala Cys Ser * 5325 5330 5335 aaa aaa aac taa tta tgt ttt act tac gat ggc aat ccc tga tgc agg 19640 Lys Lys Asn * Leu Cys Phe Thr Tyr Asp Gly Asn Pro * Cys Arg 5340 5345 5350 ttc ggt gtt ttt ata cct gtt gcg agt atg tgt gag ttt act taa agt 19688 Phe Gly Val Phe Ile Pro Val Ala Ser Met Cys Glu Phe Thr * Ser 5355 5360 5365 ttg tac ggg att ttt tgt tga tga aca ttt ctt cta tac tcg ctt ttg 19736 Leu Tyr Gly Ile Phe Cys * * Thr Phe Leu Leu Tyr Ser Leu Leu 5370 5375 aat tat tac ttg ttg ggt tca gac ctg ttc act tct gca tca taa act 19784 Asn Tyr Tyr Leu Leu Gly Ser Asp Leu Phe Thr Ser Ala Ser * Thr 5380 5385 5390 atg gtg tta ctt tag tta gac tct gat gcg aac cat att tgg aag caa 19832 Met Val Leu Leu * Leu Asp Ser Asp Ala Asn His Ile Trp Lys Gln 5395 5400 5405 tta tag ctg cca tgt aaa ttt gca ggt ggc atc ttg tca ctg taa ctt 19880 Leu * Leu Pro Cys Lys Phe Ala Gly Gly Ile Leu Ser Leu * Leu 5410 5415 5420 tag atg ccg acc tag gtg aag caa ctt cct tca ttg atg gag ttt atg 19928 * Met Pro Thr * Val Lys Gln Leu Pro Ser Leu Met Glu Phe Met 5425 5430 5435 atg gat atc aga atg ggt tgc cgt tgc caa cag ata acg gta ttt ggg 19976 Met Asp Ile Arg Met Gly Cys Arg Cys Gln Gln Ile Thr Val Phe Gly 5440 5445 5450 aac ccg gaa ctg ata ttt ggg ttg gtg cta ggc cac cca tgg act tag 20024 Asn Pro Glu Leu Ile Phe Gly Leu Val Leu Gly His Pro Trp Thr * 5455 5460 5465 atg cct ttg gta ggt cag ata gcg aag gtt ctg act caa aga tgc aga 20072 Met Pro Leu Val Gly Gln Ile Ala Lys Val Leu Thr Gln Arg Cys Arg 5470 5475 5480 tca tgg atg ctt ttc tat ggg gaa gat gtc tca gtg aag atg agg tta 20120 Ser Trp Met Leu Phe Tyr Gly Glu Asp Val Ser Val Lys Met Arg Leu 5485 5490 5495 5500 ctg ttt tac ata ctg cca tgt ctc ctg ctg agt atg gat ttt ttg acc 20168 Leu Phe Tyr Ile Leu Pro Cys Leu Leu Leu Ser Met Asp Phe Leu Thr 5505 5510 5515 ttg cac ccg gcg atg ctt ggc atg gaa gtt att ctg caa ggg tat tgc 20216 Leu His Pro Ala Met Leu Gly Met Glu Val Ile Leu Gln Gly Tyr Cys 5520 5525 5530 ttt tac cta taa att ctg cac cag ttt ttt gtg atg tct tga atc gag 20264 Phe Tyr Leu * Ile Leu His Gln Phe Phe Val Met Ser * Ile Glu 5535 5540 5545 agg gaa tat gat att gaa tgt gtt ttt ctt atg tta ctg ttt cat tca 20312 Arg Glu Tyr Asp Ile Glu Cys Val Phe Leu Met Leu Leu Phe His Ser 5550 5555 5560 ggt gga tga ctg gga aag cga aga agc tta tga gct tta tga tca agg 20360 Gly Gly * Leu Gly Lys Arg Arg Ser Leu * Ala Leu * Ser Arg 5565 5570 5575 gga tgt cga atg gga tgg aca gta ctc aag tgg tag gaa acg ccc ggt 20408 Gly Cys Arg Met Gly Trp Thr Val Leu Lys Trp * Glu Thr Pro Gly 5580 5585 5590 aca tga tgc tgt agc tat tga cct tga ctc ctt tgc tag gag acc aag 20456 Thr * Cys Cys Ser Tyr * Pro * Leu Leu Cys * Glu Thr Lys 5595 5600 aaa acc aag gtt tga gac acg tga tga agt caa cca gcg tat gct ttc 20504 Lys Thr Lys Val * Asp Thr * * Ser Gln Pro Ala Tyr Ala Phe 5605 5610 5615 tgt tga aag ggc tgt cag gga tgc tct tat cgc gaa agg aga gag aaa 20552 Cys * Lys Gly Cys Gln Gly Cys Ser Tyr Arg Glu Arg Arg Glu Lys 5620 5625 5630 ctt cac tga tca aga gtt ccc tcc aga aga tcg ttc ttt att tgt aga 20600 Leu His * Ser Arg Val Pro Ser Arg Arg Ser Phe Phe Ile Cys Arg 5635 5640 5645 tcc gat gaa tcc acc tct gaa act gca ggt atc ata tgc aca tct cat 20648 Ser Asp Glu Ser Thr Ser Glu Thr Ala Gly Ile Ile Cys Thr Ser His 5650 5655 5660 gct ctt gtg aaa aat att ggt taa gtt cct gac atg ttt taa ttt gtg 20696 Ala Leu Val Lys Asn Ile Gly * Val Pro Asp Met Phe * Phe Val 5665 5670 5675 agt ttt agg ttg ttt ctg agt gga tga ggc ctt ctg aca tag caa agg 20744 Ser Phe Arg Leu Phe Leu Ser Gly * Gly Leu Leu Thr * Gln Arg 5680 5685 aga tat cta tca gtt gtc agc ctt gct tgt ttt cgg gtt ctg tga att 20792 Arg Tyr Leu Ser Val Val Ser Leu Ala Cys Phe Arg Val Leu * Ile 5690 5695 5700 cct cag atg tgt gtc agg tat att ttt ctg ttt tct act ttt gtg cag 20840 Pro Gln Met Cys Val Arg Tyr Ile Phe Leu Phe Ser Thr Phe Val Gln 5705 5710 5715 5720 cca gaa ttg tgg taa agg gga att tag ggc atc ctt att tat tat cag 20888 Pro Glu Leu Trp * Arg Gly Ile * Gly Ile Leu Ile Tyr Tyr Gln 5725 5730 aat tga gat ccg gtt tgc aca aac ctt ttc agg gtc ggt tgg gag act 20936 Asn * Asp Pro Val Cys Thr Asn Leu Phe Arg Val Gly Trp Glu Thr 5735 5740 5745 gtt ggt tcc taa gtg cag tcg cag ttt taa ctg aga tgt ctc gga tat 20984 Val Gly Ser * Val Gln Ser Gln Phe * Leu Arg Cys Leu Gly Tyr 5750 5755 5760 cag aag tta taa tca ctc ctg agt aca atg atg aag gga ttt ata cag 21032 Gln Lys Leu * Ser Leu Leu Ser Thr Met Met Lys Gly Phe Ile Gln 5765 5770 5775 tca gat tct gta ttc agg tac cca ata ttt caa tgt tta aac cat ttg 21080 Ser Asp Ser Val Phe Arg Tyr Pro Ile Phe Gln Cys Leu Asn His Leu 5780 5785 5790 act gat aag tac ttg aat tgc tcc ttt tct att aat aca cta gcc ttg 21128 Thr Asp Lys Tyr Leu Asn Cys Ser Phe Ser Ile Asn Thr Leu Ala Leu 5795 5800 5805 5810 tgt cat ttg gtc agt atg tta tct agg aag gtt cag ttg att cat ttt 21176 Cys His Leu Val Ser Met Leu Ser Arg Lys Val Gln Leu Ile His Phe 5815 5820 5825 gca aag ttt ata ata cag tta tct taa aat gtt aga cca aaa aat cac 21224 Ala Lys Phe Ile Ile Gln Leu Ser * Asn Val Arg Pro Lys Asn His 5830 5835 5840 ggt gaa gtt tac cat ttg aca atc ata tgt cca gtt taa cat tag agt 21272 Gly Glu Val Tyr His Leu Thr Ile Ile Cys Pro Val * His * Ser 5845 5850 5855 tct att tct gaa gca aat gtg tac atc atg cta cat gca tcg ata aca 21320 Ser Ile Ser Glu Ala Asn Val Tyr Ile Met Leu His Ala Ser Ile Thr 5860 5865 5870 tat tta act gtg tca gga aaa ata aat tat ttt att agt ttt ttt gaa 21368 Tyr Leu Thr Val Ser Gly Lys Ile Asn Tyr Phe Ile Ser Phe Phe Glu 5875

5880 5885 ggg aaa acc gta gga aat tcc cta cag tga aat ata ttg aat taa att 21416 Gly Lys Thr Val Gly Asn Ser Leu Gln * Asn Ile Leu Asn * Ile 5890 5895 5900 agg aaa aat aag gac gac cac tgg aca aaa ccg aaa aag agg aaa aag 21464 Arg Lys Asn Lys Asp Asp His Trp Thr Lys Pro Lys Lys Arg Lys Lys 5905 5910 5915 gaa agg ggg gaa agg caa aga aca tcc caa att aca act cca ata act 21512 Glu Arg Gly Glu Arg Gln Arg Thr Ser Gln Ile Thr Thr Pro Ile Thr 5920 5925 5930 agc aag ata taa ggg ata att att tta tta gta gtt cat tgg tct gca 21560 Ser Lys Ile * Gly Ile Ile Ile Leu Leu Val Val His Trp Ser Ala 5935 5940 5945 ata ttt act gga gcc atg ttg gag tca tct tgg atg tgt act gct cta 21608 Ile Phe Thr Gly Ala Met Leu Glu Ser Ser Trp Met Cys Thr Ala Leu 5950 5955 5960 tgg aga tat ata ctg cat gat tgg ttg att ggg ccc cgc gcc cag gcc 21656 Trp Arg Tyr Ile Leu His Asp Trp Leu Ile Gly Pro Arg Ala Gln Ala 5965 5970 5975 5980 ccg tgc atg cgt tga ccg cgc ttg ggc gtg tgc ttg gtt ggc tgc acg 21704 Pro Cys Met Arg * Pro Arg Leu Gly Val Cys Leu Val Gly Cys Thr 5985 5990 5995 gca gcg acg gag cct gtg tgc aca ggt gca agg tag gtt ttt taa ccc 21752 Ala Ala Thr Glu Pro Val Cys Thr Gly Ala Arg * Val Phe * Pro 6000 6005 tcg ttg gct tgc atg cgt ggg gaa gta aga atc atc cgt ttc aac cga 21800 Ser Leu Ala Cys Met Arg Gly Glu Val Arg Ile Ile Arg Phe Asn Arg 6010 6015 6020 6025 tgc agg cct ccg cga gtc agg cgc gga ggc tgc tag caa cca aac agg 21848 Cys Arg Pro Pro Arg Val Arg Arg Gly Gly Cys * Gln Pro Asn Arg 6030 6035 6040 agg ata atg tat att act aaa aat agt agc tta gtt ata ctt cac ctc 21896 Arg Ile Met Tyr Ile Thr Lys Asn Ser Ser Leu Val Ile Leu His Leu 6045 6050 6055 tgc aat gta agt act tgc ttc cct caa tac ttt tta tgc cct gac ctg 21944 Cys Asn Val Ser Thr Cys Phe Pro Gln Tyr Phe Leu Cys Pro Asp Leu 6060 6065 6070 gta agt ccc tca gtg aca cac tga cac cta tct ata aac ctg agg ttg 21992 Val Ser Pro Ser Val Thr His * His Leu Ser Ile Asn Leu Arg Leu 6075 6080 6085 gta aga aaa tac ctt tct gtt act gat ccc atg tgg ttt ccg tgt gtt 22040 Val Arg Lys Tyr Leu Ser Val Thr Asp Pro Met Trp Phe Pro Cys Val 6090 6095 6100 gtt gat ata gat ttc agg agt gat atc ttt ata tca caa tac aaa ttg 22088 Val Asp Ile Asp Phe Arg Ser Asp Ile Phe Ile Ser Gln Tyr Lys Leu 6105 6110 6115 gct gta gtt ggt gta acc cag aag gga aaa aca gtg cac atg aag agc 22136 Ala Val Val Gly Val Thr Gln Lys Gly Lys Thr Val His Met Lys Ser 6120 6125 6130 6135 act tgt aat atg ttt ttt att agg gct taa cac taa tgc att gtt ttc 22184 Thr Cys Asn Met Phe Phe Ile Arg Ala * His * Cys Ile Val Phe 6140 6145 ccc tct gac tta ata ttt agc tta cat tgt gga gac tag aga tgt ttt 22232 Pro Ser Asp Leu Ile Phe Ser Leu His Cys Gly Asp * Arg Cys Phe 6150 6155 6160 atg aat tat ctt aca cta atc tga ttt tct ctg tat ttt aaa aaa ctc 22280 Met Asn Tyr Leu Thr Leu Ile * Phe Ser Leu Tyr Phe Lys Lys Leu 6165 6170 6175 ttc tct gag tta tag ttc tta tgt ttt ata ttt tcc caa ggg tga gtg 22328 Phe Ser Glu Leu * Phe Leu Cys Phe Ile Phe Ser Gln Gly * Val 6180 6185 6190 ggt ggc tgt ggt tgt tga tga ttg gat tcc ttg cga gtc tcc ggg gaa 22376 Gly Gly Cys Gly Cys * * Leu Asp Ser Leu Arg Val Ser Gly Glu 6195 6200 6205 acc agc att tgc tac tag tag aaa gca aaa cga gct ttg ggt atc cat 22424 Thr Ser Ile Cys Tyr * * Lys Ala Lys Arg Ala Leu Gly Ile His 6210 6215 6220 tct tga gaa ggc tta tgc aaa act tca tgg ctc tta tga ggc att gga 22472 Ser * Glu Gly Leu Cys Lys Thr Ser Trp Leu Leu * Gly Ile Gly 6225 6230 6235 agg tgg gct tgt tca aga tgc tct agt cga tct cac agg agg agc tgg 22520 Arg Trp Ala Cys Ser Arg Cys Ser Ser Arg Ser His Arg Arg Ser Trp 6240 6245 6250 tga aga gat tga tat gcg aag tcc tca agc cca act tga tct tgc tag 22568 * Arg Asp * Tyr Ala Lys Ser Ser Ser Pro Thr * Ser Cys * 6255 6260 tgg aag att gtg gtc gca gtt gtt gca ttt caa aca aga agg ttt tct 22616 Trp Lys Ile Val Val Ala Val Val Ala Phe Gln Thr Arg Arg Phe Ser 6265 6270 6275 tct tgg tgc tgg aag tcc ttc tgg atc tga tgc tca cat ctc atc aag 22664 Ser Trp Cys Trp Lys Ser Phe Trp Ile * Cys Ser His Leu Ile Lys 6280 6285 6290 tgg cat tgt tca ggg aca tgc gta ctc aat ttt gca ggt ttg ctc tag 22712 Trp His Cys Ser Gly Thr Cys Val Leu Asn Phe Ala Gly Leu Leu * 6295 6300 6305 ttt aga taa ttc tat ctg aga tac tgg gta act gtc agc ttt tca cta 22760 Phe Arg * Phe Tyr Leu Arg Tyr Trp Val Thr Val Ser Phe Ser Leu 6310 6315 6320 ctc ccg gga caa gtt tac aca att ata cat tat ttc agg taa gag aag 22808 Leu Pro Gly Gln Val Tyr Thr Ile Ile His Tyr Phe Arg * Glu Lys 6325 6330 6335 ttg atg gcc aca aac tca tcc aaa tca gaa atc cat ggg caa atg aag 22856 Leu Met Ala Thr Asn Ser Ser Lys Ser Glu Ile His Gly Gln Met Lys 6340 6345 6350 6355 ttg aat gga atg gac cat ggt cag act cgt cac cag agt gga cgg aac 22904 Leu Asn Gly Met Asp His Gly Gln Thr Arg His Gln Ser Gly Arg Asn 6360 6365 6370 gga tga agc ata agc tca tgc atg ttc cac agg tac ttg tct ctt gat 22952 Gly * Ser Ile Ser Ser Cys Met Phe His Arg Tyr Leu Ser Leu Asp 6375 6380 6385 att ttt ttt gca tgt cat atc aca cta gtt ggt ggc aaa cca cag ctt 23000 Ile Phe Phe Ala Cys His Ile Thr Leu Val Gly Gly Lys Pro Gln Leu 6390 6395 6400 att gat aat cga gat aat tca ttg cac cgc tat aaa caa aat gta cca 23048 Ile Asp Asn Arg Asp Asn Ser Leu His Arg Tyr Lys Gln Asn Val Pro 6405 6410 6415 tgt aag ttt gat ttc atc tct aca gat ctt gac ttc aat ccc cct tgt 23096 Cys Lys Phe Asp Phe Ile Ser Thr Asp Leu Asp Phe Asn Pro Pro Cys 6420 6425 6430 ctc tcc atc ctt gca gtc gaa gaa tgg ggt att ctg gat gtc ttg gca 23144 Leu Ser Ile Leu Ala Val Glu Glu Trp Gly Ile Leu Asp Val Leu Ala 6435 6440 6445 6450 aga ttt tca gat tca ctt tcg gtc aat cta tgt ttg tcg tgt tta tcc 23192 Arg Phe Ser Asp Ser Leu Ser Val Asn Leu Cys Leu Ser Cys Leu Ser 6455 6460 6465 acc tga gat gcg tta ctc tgt cca tgg gca atg gcg tgg tta caa tgc 23240 Thr * Asp Ala Leu Leu Cys Pro Trp Ala Met Ala Trp Leu Gln Cys 6470 6475 6480 agg tgg ttg cca aga tta tga ctc atg gca cca aaa tcc aca gta tcg 23288 Arg Trp Leu Pro Arg Leu * Leu Met Ala Pro Lys Ser Thr Val Ser 6485 6490 6495 act tag agt aac agg acg tga tgc act ata ccc tgt tca tgt ttt tat 23336 Thr * Ser Asn Arg Thr * Cys Thr Ile Pro Cys Ser Cys Phe Tyr 6500 6505 6510 tac cct tac tca ggt atg ttg gaa cct acc tat caa cct aac acc agg 23384 Tyr Pro Tyr Ser Gly Met Leu Glu Pro Thr Tyr Gln Pro Asn Thr Arg 6515 6520 6525 aga agc aca atg atg ttt tat ctg tgt aaa ttt cat gct ttt cag ggt 23432 Arg Ser Thr Met Met Phe Tyr Leu Cys Lys Phe His Ala Phe Gln Gly 6530 6535 6540 gtt ggt ttc tct aga aag acg aat ggt ttt cgg aac tac caa tct agc 23480 Val Gly Phe Ser Arg Lys Thr Asn Gly Phe Arg Asn Tyr Gln Ser Ser 6545 6550 6555 cat gat tct tca atg ttt tac att gga atg agg ata ctc aag aca cag 23528 His Asp Ser Ser Met Phe Tyr Ile Gly Met Arg Ile Leu Lys Thr Gln 6560 6565 6570 ggc tgc cgt gct gct tac aat atc tac atg cat gaa agc gct ggt gga 23576 Gly Cys Arg Ala Ala Tyr Asn Ile Tyr Met His Glu Ser Ala Gly Gly 6575 6580 6585 6590 aca gat tac gtt aac tcg agg gag ata tca tgc gaa ctg gtc ttg gat 23624 Thr Asp Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Leu Val Leu Asp 6595 6600 6605 cct tat ccc aaa ggg tac aca att gtg cca act acc atc cac cct ggg 23672 Pro Tyr Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile His Pro Gly 6610 6615 6620 gag gaa gca cct ttt gtt ttg tca gtt ttt tca aaa gca tca atc aga 23720 Glu Glu Ala Pro Phe Val Leu Ser Val Phe Ser Lys Ala Ser Ile Arg 6625 6630 6635 cta gag gct gtt t aattcaagat tgagatccca tgtgtttggt ggtagctgcg 23773 Leu Glu Ala Val 6640 tctgctgggc actcgtgcac gcaggatcca gctgtgggtt ctcgtgaact agataattgg 23833 taagtgttgt attgagttac ctgtacagaa gtatgaagtg gtagttaacc cagggtgccc 23893 caaactacaa ccatcctata tttcacgtgc cgctatattc tcatcattca ttcaggttca 23953 aaacagagga gttggactaa ctggccttat aaccgtttca gggtatagga attgcctcct 24013 ggacaacttc aatgaatctt gctgcatgca agtacataag ttcggttgct tgttgcagaa 24073 ctgacaaacg gcaatgcttc ttgtgctgaa gggaaaggag agaaggcatg atccatggtt 24133 ctttggtagc tgcgcaaagt gcagggtgag aggcttggtt caatgtttgt agatagccgt 24193 ggtaactgac ctggtagccc atcctatgta taggtgtccc gtttaccctg taaatgctat 24253 agagttaggt taggtagcct gtcgttcctg ttaacgcata gggctcttat gcagctgtga 24313 aatgtcttgt tagcaagctg cagttttgct gatttgagcg tggagtagtc ggccatagct 24373 gttcccattg gtttgccctg tatgtaatcg gaatctgatg tcattcaatg aacctatttt 24433 ttgggtgcca tgcgaagctg tctaagattt gagtctcttc atgcatcaaa ttatgcatca 24493 tcctcgctgg aaaaataaaa actctaccaa acatagacga ttatttggtt ccttttctcg 24553 tcgatgtttc cttatgacca aagttgttag gtttgatagt tcgatggcca agataatttg 24613 agaaaaaaat gacttcttcc attttgttat tattaataac ctattaccag tttcggggca 24673 atccaagttc caatcagtgc acacattagt taattttgta ctgcattttt ttatcaaata 24733 taaaaaaagt agtagtcaaa atagcataaa ccaaccaaca aactaaacca tggtacgtac 24793 atgttagtgc tacgtcatat cgacaacgca gcctgccgat catatatcat cttccttaga 24853 tgtcagtgta tcctgcccga ttgctgctgc ggcagcagcc ccgggatgaa tggcacgccg 24913 agctgtggga gccagtgctg gccctggatg aagctctcga cggtgtactt ctgcagcgcg 24973 tgctggtagg tgatgttctt gatacccctc cacttgacgc gttcgctcat gttggcgccg 25033 ggcccgtggt tttccacctc ggcgtagtag caggtgctga ggccgaagtc gccgagccac 25093 ggcagccagc cctgcgggtc gatgaacccg ccgatctccg actggatgta gagcgtgcgc 25153 gagtgctcct tccacgggcg gcccaggaag gtcctgaacc ggccgacgct cttctcgaac 25213 tccgggtgcg gcgcgacggt gcagttgtgg atgacggtgc cgcccaccga ccgcctctcc 25273 ttgcggccct gcgccgtgac gatgttctgc tggttgtcca tgcacttgcg cacctggatg 25333 aggcagttct ggaacaccac ctgcgcgttg ccgaagatga agtcgatggt gcccgtgatg 25393 acgcagtcgc ggtagtac 25411 26 7094 DNA Arabidopsis thaliana CDS (120)...(6570) cDNA 26 cttggttggt tttaagctgc ggatttgatg atttgtgcgc aagcttgggg tttcagcttt 60 tttttgtgat ggaattttga tttccgagtt gcatggtgtt gtaggtggga gaagaagcc 119 atg gaa ggg gat gag cga gga gtc tta ctt gct tgt gta att tcg ggt 167 Met Glu Gly Asp Glu Arg Gly Val Leu Leu Ala Cys Val Ile Ser Gly 1 5 10 15 acc ctt ttc acg gtt ttc ggt tcg ggt tcg ttt tgg ata ctt tgg gct 215 Thr Leu Phe Thr Val Phe Gly Ser Gly Ser Phe Trp Ile Leu Trp Ala 20 25 30 gtt aat tgg cgg cca tgg cgt ctc tac agt tgg atc ttt gct aga aaa 263 Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Phe Ala Arg Lys 35 40 45 tgg cca aaa gta ttg caa ggt cct cag ctt gat ata cta tgt ggt gtt 311 Trp Pro Lys Val Leu Gln Gly Pro Gln Leu Asp Ile Leu Cys Gly Val 50 55 60 cta tct ctt ttt gct tgg att gtg gta gta tcc cct att gca atc ttg 359 Leu Ser Leu Phe Ala Trp Ile Val Val Val Ser Pro Ile Ala Ile Leu 65 70 75 80 ata gga tgg ggt tct tgg ctg att gtg ata ttg gat cga cat atc att 407 Ile Gly Trp Gly Ser Trp Leu Ile Val Ile Leu Asp Arg His Ile Ile 85 90 95 ggg ctg gcg ata ata atg gct gga aca gcc ctt tta ctg gca ttc tac 455 Gly Leu Ala Ile Ile Met Ala Gly Thr Ala Leu Leu Leu Ala Phe Tyr 100 105 110 tca atc atg ctt tgg tgg agg acc cag tgg caa agc tca aga gct gtc 503 Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Arg Ala Val 115 120 125 gct tta ctt ctc ctt ctt ggt gtt gcc tta cta tgt gcg tat gaa ctc 551 Ala Leu Leu Leu Leu Leu Gly Val Ala Leu Leu Cys Ala Tyr Glu Leu 130 135 140 tgt gct gtc tat gtt acg gct ggt gcg cat gca tct cag caa tat tct 599 Cys Ala Val Tyr Val Thr Ala Gly Ala His Ala Ser Gln Gln Tyr Ser 145 150 155 160 cct tct ggt ttc ttt ttc ggt gta tca gca atc gcg ttg gca att aac 647 Pro Ser Gly Phe Phe Phe Gly Val Ser Ala Ile Ala Leu Ala Ile Asn 165 170 175 atg cta ttt atc tgc cgc atg gtc ttt aat gga aat ggt tta gat gtg 695 Met Leu Phe Ile Cys Arg Met Val Phe Asn Gly Asn Gly Leu Asp Val 180 185 190 gac gaa tat gta agg agg gca tat aaa ttt gct tat tca gat tgt ata 743 Asp Glu Tyr Val Arg Arg Ala Tyr Lys Phe Ala Tyr Ser Asp Cys Ile 195 200 205 gaa gtg ggt cct gtg gct tgt ttg cct gaa cct cct gat cct aat gaa 791 Glu Val Gly Pro Val Ala Cys Leu Pro Glu Pro Pro Asp Pro Asn Glu 210 215 220 tta tat ccc cgg caa acc agc agg gct tca cat ctt ggc ctt ctg tac 839 Leu Tyr Pro Arg Gln Thr Ser Arg Ala Ser His Leu Gly Leu Leu Tyr 225 230 235 240 ctg ggc tca ctc gta gtt ctc ctt gcc tac tca gtc cta tat ggt ctc 887 Leu Gly Ser Leu Val Val Leu Leu Ala Tyr Ser Val Leu Tyr Gly Leu 245 250 255 aca gct agg gaa tca cgt tgg ctt gga gga atc aca tca gct gca gtt 935 Thr Ala Arg Glu Ser Arg Trp Leu Gly Gly Ile Thr Ser Ala Ala Val 260 265 270 att gtt ctt gac tgg aat att ggg gca tgc ttg tat ggg ttt aag ctt 983 Ile Val Leu Asp Trp Asn Ile Gly Ala Cys Leu Tyr Gly Phe Lys Leu 275 280 285 ctt cag aat cgt gtt ctg gca ctt ttt gtt gct ggc ata tcc cgt ctt 1031 Leu Gln Asn Arg Val Leu Ala Leu Phe Val Ala Gly Ile Ser Arg Leu 290 295 300 ttc cta ata tgt ttt ggc ata cac tac tgg tac cta ggg cat tgt att 1079 Phe Leu Ile Cys Phe Gly Ile His Tyr Trp Tyr Leu Gly His Cys Ile 305 310 315 320 agt tac att ttc gta gca tca gtt cta tca ggt gct gct gtt tct cgg 1127 Ser Tyr Ile Phe Val Ala Ser Val Leu Ser Gly Ala Ala Val Ser Arg 325 330 335 cat cta tct ata aca gac cca tca gct gca aga aga gat gcc tta cag 1175 His Leu Ser Ile Thr Asp Pro Ser Ala Ala Arg Arg Asp Ala Leu Gln 340 345 350 agc aca gtg atc cgc ttg aga gaa ggt ttt cgg aga aaa gag cag aat 1223 Ser Thr Val Ile Arg Leu Arg Glu Gly Phe Arg Arg Lys Glu Gln Asn 355 360 365 agt tct tca agt tct tca gat ggt tgt ggc tca agt ata aaa aga agt 1271 Ser Ser Ser Ser Ser Ser Asp Gly Cys Gly Ser Ser Ile Lys Arg Ser 370 375 380 agt agt atc gat gct ggc cat act ggt tgt act aat gaa gca aat cgt 1319 Ser Ser Ile Asp Ala Gly His Thr Gly Cys Thr Asn Glu Ala Asn Arg 385 390 395 400 acg gca gaa tct tgc acg gct gac aat cta act cga aca ggc agc tct 1367 Thr Ala Glu Ser Cys Thr Ala Asp Asn Leu Thr Arg Thr Gly Ser Ser 405 410 415 cag gag gga atc aat agc gac aaa agc gaa gaa agt gga aga cca agc 1415 Gln Glu Gly Ile Asn Ser Asp Lys Ser Glu Glu Ser Gly Arg Pro Ser 420 425 430 tta ggt tta cgt agt agt tca tgt cgt tct gtg gtc caa gag ccc gaa 1463 Leu Gly Leu Arg Ser Ser Ser Cys Arg Ser Val Val Gln Glu Pro Glu 435 440 445 gca gga acg tct tat ttt atg gac aaa gtt tct gat caa aat aac act 1511 Ala Gly Thr Ser Tyr Phe Met Asp Lys Val Ser Asp Gln Asn Asn Thr 450 455 460 ctt gtt gtt tgt tcg agc agt ggt cta gat agc caa ggt tac gag tct 1559 Leu Val Val Cys Ser Ser Ser Gly Leu Asp Ser Gln Gly Tyr Glu Ser 465 470 475 480 agc aca tcg aat tct gca aac cag cag ctt ttg gat atg aat ttg gct 1607 Ser Thr Ser Asn Ser Ala Asn Gln Gln Leu Leu Asp Met Asn Leu Ala 485 490 495 ctt gct ttc cag gac cag tta aac aat cct agg ata gcc tcg ata ctt 1655 Leu Ala Phe Gln Asp Gln Leu Asn Asn Pro Arg Ile Ala Ser Ile Leu 500 505 510 aag aag aaa gca aaa gaa ggt gat ctt gaa ctg act aat ttg ctg caa 1703 Lys Lys Lys Ala Lys Glu Gly Asp Leu Glu Leu Thr Asn Leu Leu Gln 515 520 525 gac aag ggg ttg gac cct aac ttt gct gta atg ttg aag gaa aaa aac 1751 Asp Lys Gly Leu Asp Pro Asn

Phe Ala Val Met Leu Lys Glu Lys Asn 530 535 540 ttg gat cct act ata ttg gca cta ctt cag agg agt agt ttg gat gca 1799 Leu Asp Pro Thr Ile Leu Ala Leu Leu Gln Arg Ser Ser Leu Asp Ala 545 550 555 560 gat aga gat cac cgc gac aat act gat att aca atc att gac tca aac 1847 Asp Arg Asp His Arg Asp Asn Thr Asp Ile Thr Ile Ile Asp Ser Asn 565 570 575 agt gtt gac aat act ttg cca aat cag att tct tta tcc gaa gaa ttg 1895 Ser Val Asp Asn Thr Leu Pro Asn Gln Ile Ser Leu Ser Glu Glu Leu 580 585 590 aga ctc cgt gga cta gag aag tgg ctt aag ttg tct aga ctt ctt ctg 1943 Arg Leu Arg Gly Leu Glu Lys Trp Leu Lys Leu Ser Arg Leu Leu Leu 595 600 605 cac cat gta gcg ggg aca cca gag aga gca tgg ggc ctc ttt agt ctt 1991 His His Val Ala Gly Thr Pro Glu Arg Ala Trp Gly Leu Phe Ser Leu 610 615 620 gtc ttt atc ctt gaa aca atc att gtg gcc att ttt cgc cca aag acc 2039 Val Phe Ile Leu Glu Thr Ile Ile Val Ala Ile Phe Arg Pro Lys Thr 625 630 635 640 atc acg att ata aat tct agt cat caa cag ttc gaa ttt ggt ttc tct 2087 Ile Thr Ile Ile Asn Ser Ser His Gln Gln Phe Glu Phe Gly Phe Ser 645 650 655 gtg ctg cta ttg tca cct gtt gtc tgt tca ata atg gct ttt ctt cgg 2135 Val Leu Leu Leu Ser Pro Val Val Cys Ser Ile Met Ala Phe Leu Arg 660 665 670 tct ctt caa gtt gag gaa atg gcc ttg aca tca aaa tct cgc aag tat 2183 Ser Leu Gln Val Glu Glu Met Ala Leu Thr Ser Lys Ser Arg Lys Tyr 675 680 685 ggc ttt gtt gcc tgg ctt ctg agc aca tca gtt gga ttg tca ctc tcg 2231 Gly Phe Val Ala Trp Leu Leu Ser Thr Ser Val Gly Leu Ser Leu Ser 690 695 700 ttc ttg agt aaa tcg tca gta ctt ctg gga ata tcc ttg act gtg ccc 2279 Phe Leu Ser Lys Ser Ser Val Leu Leu Gly Ile Ser Leu Thr Val Pro 705 710 715 720 ctc atg gca gca tgc ctg tct att gct gtt ccc ata tgg atg cat aat 2327 Leu Met Ala Ala Cys Leu Ser Ile Ala Val Pro Ile Trp Met His Asn 725 730 735 ggg tac caa ttt tgg gtt cca cag tta tca tgt ggt gac cag gca aga 2375 Gly Tyr Gln Phe Trp Val Pro Gln Leu Ser Cys Gly Asp Gln Ala Arg 740 745 750 gat tta cga tct ccc agg ata aag ggg ttt att ctt tgg att tgt gtt 2423 Asp Leu Arg Ser Pro Arg Ile Lys Gly Phe Ile Leu Trp Ile Cys Val 755 760 765 gtg ttg ttt gcg ggt tct gta att tct ctt ggt gcg att ata tct gct 2471 Val Leu Phe Ala Gly Ser Val Ile Ser Leu Gly Ala Ile Ile Ser Ala 770 775 780 aaa cct ttg gat gat tta aag tat aag ctg ttt agt gcc aga gaa aac 2519 Lys Pro Leu Asp Asp Leu Lys Tyr Lys Leu Phe Ser Ala Arg Glu Asn 785 790 795 800 aac gtc acg tca cca tat aca tct tct gta tac ctt ggt tgg gca atg 2567 Asn Val Thr Ser Pro Tyr Thr Ser Ser Val Tyr Leu Gly Trp Ala Met 805 810 815 tca tct gga att gct tta gta gtt acc gcc att cta cca ata gtt tca 2615 Ser Ser Gly Ile Ala Leu Val Val Thr Ala Ile Leu Pro Ile Val Ser 820 825 830 tgg ttt gca act tat agg ttt tcc cac tct tct gct gtc tgt ctc atg 2663 Trp Phe Ala Thr Tyr Arg Phe Ser His Ser Ser Ala Val Cys Leu Met 835 840 845 ata ttc tca gtt gtt ctc gtg gca ttt tgt gga act tca tat ttg gaa 2711 Ile Phe Ser Val Val Leu Val Ala Phe Cys Gly Thr Ser Tyr Leu Glu 850 855 860 gtt gta aaa tct aga gat gat cag ttg ccc aca aag ggt gat ttc ctt 2759 Val Val Lys Ser Arg Asp Asp Gln Leu Pro Thr Lys Gly Asp Phe Leu 865 870 875 880 gcg gcc ttg ctt cca ctt gca tgc att ccg gcg ctg ctt tca cta tgc 2807 Ala Ala Leu Leu Pro Leu Ala Cys Ile Pro Ala Leu Leu Ser Leu Cys 885 890 895 tgt ggg atg gtt aaa tgg aag gac gat tgt tgg ata ctc tct cga ggt 2855 Cys Gly Met Val Lys Trp Lys Asp Asp Cys Trp Ile Leu Ser Arg Gly 900 905 910 gta tat gtt ttc ttt tca ata ggt ctt ctt ctt ctt ttt ggt gcg ata 2903 Val Tyr Val Phe Phe Ser Ile Gly Leu Leu Leu Leu Phe Gly Ala Ile 915 920 925 gca gct gtc att gca gtc aaa cca tgg acg ata ggc gta tct ttt ctc 2951 Ala Ala Val Ile Ala Val Lys Pro Trp Thr Ile Gly Val Ser Phe Leu 930 935 940 tta gtt ctt ttc ctt atg gtg gta aca att ggt gta atc cat ctt tgg 2999 Leu Val Leu Phe Leu Met Val Val Thr Ile Gly Val Ile His Leu Trp 945 950 955 960 gcg tca aac aat ttc tat tta acc agg aaa cag aca tcc ttt gtc tgc 3047 Ala Ser Asn Asn Phe Tyr Leu Thr Arg Lys Gln Thr Ser Phe Val Cys 965 970 975 ttt ctt gct ctt ctt ttg ggt ttg gcc gca ttc ctt ctc gga tgg cat 3095 Phe Leu Ala Leu Leu Leu Gly Leu Ala Ala Phe Leu Leu Gly Trp His 980 985 990 caa gat aaa gca ttt gct gga gca tct gtt ggt tac ttt aca ttc ctg 3143 Gln Asp Lys Ala Phe Ala Gly Ala Ser Val Gly Tyr Phe Thr Phe Leu 995 1000 1005 tct ctg ttg gct gga aga gca tta gct gtt ctt cta tcc cca cca att 3191 Ser Leu Leu Ala Gly Arg Ala Leu Ala Val Leu Leu Ser Pro Pro Ile 1010 1015 1020 gta gta tat tct cca agg gtg cta cca gta tat gtc tac gat gct cat 3239 Val Val Tyr Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His 1025 1030 1035 1040 gct gat tgc gga aag aat gtc agt gct gca ttt ctt gtc ctg tat gga 3287 Ala Asp Cys Gly Lys Asn Val Ser Ala Ala Phe Leu Val Leu Tyr Gly 1045 1050 1055 att gct ttg gca aca gaa ggc tgg ggt gtt gtt gct agt ctg ata att 3335 Ile Ala Leu Ala Thr Glu Gly Trp Gly Val Val Ala Ser Leu Ile Ile 1060 1065 1070 tat cct ccg ttt gcg ggt gct gct gta tca gct atc acc ctt gta gta 3383 Tyr Pro Pro Phe Ala Gly Ala Ala Val Ser Ala Ile Thr Leu Val Val 1075 1080 1085 gcc ttt ggg ttt gct gtt tct cgc cca tgt ttg act ctt gag atg atg 3431 Ala Phe Gly Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Glu Met Met 1090 1095 1100 gag gtt gct gta cgc ttt ctt agc aag gat act ata gtg caa gct atc 3479 Glu Val Ala Val Arg Phe Leu Ser Lys Asp Thr Ile Val Gln Ala Ile 1105 1110 1115 1120 tct cga tct gcc acg aaa aca aga aat gct cta tcc ggc acg tat tca 3527 Ser Arg Ser Ala Thr Lys Thr Arg Asn Ala Leu Ser Gly Thr Tyr Ser 1125 1130 1135 gct ccc caa agg tcc gcc agc tct gca gct ctt ctg gtt ggg gat ccc 3575 Ala Pro Gln Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro 1140 1145 1150 tct gca atg cgt gat aaa gca ggg aac ttt gtg ctt cct aga gat gat 3623 Ser Ala Met Arg Asp Lys Ala Gly Asn Phe Val Leu Pro Arg Asp Asp 1155 1160 1165 gtc atg aaa tta agg gat cgt ctc agg aac gaa gaa aga gtt gct gga 3671 Val Met Lys Leu Arg Asp Arg Leu Arg Asn Glu Glu Arg Val Ala Gly 1170 1175 1180 tca atc ttc tac aaa atg caa tgc agg aaa gga ttc cgt cat gaa cca 3719 Ser Ile Phe Tyr Lys Met Gln Cys Arg Lys Gly Phe Arg His Glu Pro 1185 1190 1195 1200 cct aca aat gta gat tat aga aga gac atg tgt gcc cat gca aga gtt 3767 Pro Thr Asn Val Asp Tyr Arg Arg Asp Met Cys Ala His Ala Arg Val 1205 1210 1215 ttg gca ctg gaa gag gca att gat aca gaa tgg gtg tat atg tgg gac 3815 Leu Ala Leu Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp 1220 1225 1230 aaa ttt ggt ggt tat tta cta cta ttg tta ggt ttg aca gct aag gcg 3863 Lys Phe Gly Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala 1235 1240 1245 gag aga gtt cag gat gag gta cgg ttg cgg ctc ttc tta gat agc att 3911 Glu Arg Val Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile 1250 1255 1260 ggg ttc tcg gat tta agt gcc aga aaa atc agt aaa tgg aag cca gag 3959 Gly Phe Ser Asp Leu Ser Ala Arg Lys Ile Ser Lys Trp Lys Pro Glu 1265 1270 1275 1280 gat aga aga caa ttc gaa att att caa gag agt tat ctg aga gag aaa 4007 Asp Arg Arg Gln Phe Glu Ile Ile Gln Glu Ser Tyr Leu Arg Glu Lys 1285 1290 1295 gag atg gaa gag gaa agc ctt atg cag aga cgt gaa gaa gaa ggg aga 4055 Glu Met Glu Glu Glu Ser Leu Met Gln Arg Arg Glu Glu Glu Gly Arg 1300 1305 1310 ggt aaa gaa aga agg aaa gct ctt ttg gag aag gaa gag cgc aaa tgg 4103 Gly Lys Glu Arg Arg Lys Ala Leu Leu Glu Lys Glu Glu Arg Lys Trp 1315 1320 1325 aag gaa att gaa gcg tcc ctt att cca tct att cct aat gct ggt agc 4151 Lys Glu Ile Glu Ala Ser Leu Ile Pro Ser Ile Pro Asn Ala Gly Ser 1330 1335 1340 agg gag gca gca gcc atg gca gct gca ata cgt gct gtt ggg ggt gat 4199 Arg Glu Ala Ala Ala Met Ala Ala Ala Ile Arg Ala Val Gly Gly Asp 1345 1350 1355 1360 tct gtc ctt gag gat tcc ttc gca aga gag agg gtc tcg ggt att gca 4247 Ser Val Leu Glu Asp Ser Phe Ala Arg Glu Arg Val Ser Gly Ile Ala 1365 1370 1375 cgt agg ata cgc act gct caa cta gaa cga cgt gca caa cag act gga 4295 Arg Arg Ile Arg Thr Ala Gln Leu Glu Arg Arg Ala Gln Gln Thr Gly 1380 1385 1390 ata tct ggg gca gtt tgt gtt ctt gat gat gaa cca atg ata agt ggt 4343 Ile Ser Gly Ala Val Cys Val Leu Asp Asp Glu Pro Met Ile Ser Gly 1395 1400 1405 aaa cat tgc ggc caa atg gac tca agt gtc tgt caa agt cag aag att 4391 Lys His Cys Gly Gln Met Asp Ser Ser Val Cys Gln Ser Gln Lys Ile 1410 1415 1420 agc ttt tcc gtt aca gca atg atc caa tcc gat tct gga cct gta tgt 4439 Ser Phe Ser Val Thr Ala Met Ile Gln Ser Asp Ser Gly Pro Val Cys 1425 1430 1435 1440 ctt ttt ggc act gaa ttt caa aag aaa gta tgt tgg gag att ctg gtt 4487 Leu Phe Gly Thr Glu Phe Gln Lys Lys Val Cys Trp Glu Ile Leu Val 1445 1450 1455 gct ggt tct gag caa gga att gag gct ggc caa gtt ggg ctt agg ttg 4535 Ala Gly Ser Glu Gln Gly Ile Glu Ala Gly Gln Val Gly Leu Arg Leu 1460 1465 1470 ata aca aaa ggt gag agg cag aca acc gtt gct aga gag tgg tat att 4583 Ile Thr Lys Gly Glu Arg Gln Thr Thr Val Ala Arg Glu Trp Tyr Ile 1475 1480 1485 ggt gca acc agc ata act gat gga agg tgg cat aca gtg aca atc aca 4631 Gly Ala Thr Ser Ile Thr Asp Gly Arg Trp His Thr Val Thr Ile Thr 1490 1495 1500 att gat gct gat gcg ggg gaa gct act tgt tac ata gat ggt ggg ttt 4679 Ile Asp Ala Asp Ala Gly Glu Ala Thr Cys Tyr Ile Asp Gly Gly Phe 1505 1510 1515 1520 gat ggc tac cag aat ggg tta cct cta agt att ggc agt gcc att tgg 4727 Asp Gly Tyr Gln Asn Gly Leu Pro Leu Ser Ile Gly Ser Ala Ile Trp 1525 1530 1535 gaa caa gga gct gaa gtt tgg ttg ggt gtt agg cca cct ata gat gtt 4775 Glu Gln Gly Ala Glu Val Trp Leu Gly Val Arg Pro Pro Ile Asp Val 1540 1545 1550 gat gca ttc ggg aga tca gat agt gat ggc gtc gaa tca aag atg cat 4823 Asp Ala Phe Gly Arg Ser Asp Ser Asp Gly Val Glu Ser Lys Met His 1555 1560 1565 att atg gat gtt ttc ctt tgg ggg aaa tgc tta agt gaa gaa gag gcc 4871 Ile Met Asp Val Phe Leu Trp Gly Lys Cys Leu Ser Glu Glu Glu Ala 1570 1575 1580 gct tct ttg cat gca gcc att ggc atg gct gac tta gac atg att gat 4919 Ala Ser Leu His Ala Ala Ile Gly Met Ala Asp Leu Asp Met Ile Asp 1585 1590 1595 1600 ttg tct gat gac aat tgg caa tgg acg gat tca ccc ccc aga gtc gat 4967 Leu Ser Asp Asp Asn Trp Gln Trp Thr Asp Ser Pro Pro Arg Val Asp 1605 1610 1615 ggt tgg gat agt gat cct gcc gat gtt gat ctc tat gat agg gat gac 5015 Gly Trp Asp Ser Asp Pro Ala Asp Val Asp Leu Tyr Asp Arg Asp Asp 1620 1625 1630 gta gat tgg gat gga caa tat tcc agt ggg agg aaa aga aga tca ggt 5063 Val Asp Trp Asp Gly Gln Tyr Ser Ser Gly Arg Lys Arg Arg Ser Gly 1635 1640 1645 cgg gat ttt gta atg agt gtc gat tcc ttt gcc agg aga cac agg aaa 5111 Arg Asp Phe Val Met Ser Val Asp Ser Phe Ala Arg Arg His Arg Lys 1650 1655 1660 ccc agg atg gag aca caa gaa gat ata aat caa aga atg cgt tca gtt 5159 Pro Arg Met Glu Thr Gln Glu Asp Ile Asn Gln Arg Met Arg Ser Val 1665 1670 1675 1680 gag ttg gct gtc aaa gaa gct ctc tct gca cga ggt gat aag caa ttt 5207 Glu Leu Ala Val Lys Glu Ala Leu Ser Ala Arg Gly Asp Lys Gln Phe 1685 1690 1695 act gac cag gaa ttt cct cca aat gat cgc tct tta ttt gtg gat aca 5255 Thr Asp Gln Glu Phe Pro Pro Asn Asp Arg Ser Leu Phe Val Asp Thr 1700 1705 1710 caa aat ccc cca tca aaa ttg cag gtt gtt tct gaa tgg atg aga cct 5303 Gln Asn Pro Pro Ser Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro 1715 1720 1725 gac tcc att gtg aaa gaa aac ggt agt gat tcc cgt ccc tgc ctg ttc 5351 Asp Ser Ile Val Lys Glu Asn Gly Ser Asp Ser Arg Pro Cys Leu Phe 1730 1735 1740 tct ggg gat gca aat cct tca gat gtt tgc cag ggg cgt ttg ggg gat 5399 Ser Gly Asp Ala Asn Pro Ser Asp Val Cys Gln Gly Arg Leu Gly Asp 1745 1750 1755 1760 tgt tgg ttc tta agc gcc gtt gca gtt ttg aca gag gtt tca cga ata 5447 Cys Trp Phe Leu Ser Ala Val Ala Val Leu Thr Glu Val Ser Arg Ile 1765 1770 1775 tct gaa gtg atc att act cct gaa tac aac gag gaa ggg atc tac act 5495 Ser Glu Val Ile Ile Thr Pro Glu Tyr Asn Glu Glu Gly Ile Tyr Thr 1780 1785 1790 gtt cgt ttt tgt att cag ggt gag tgg gtt cct gtt gtt atc gat gac 5543 Val Arg Phe Cys Ile Gln Gly Glu Trp Val Pro Val Val Ile Asp Asp 1795 1800 1805 tgg att cca tgt gaa tca cct ggt aaa cca gct ttt gct act agc aga 5591 Trp Ile Pro Cys Glu Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg 1810 1815 1820 aag ctc aat gaa ctc tgg gtc tcc atg gtg gag aaa gca tat gcc aag 5639 Lys Leu Asn Glu Leu Trp Val Ser Met Val Glu Lys Ala Tyr Ala Lys 1825 1830 1835 1840 ctc cat ggt tct tat gag gca ctg gag ggg gga ctg gtt cag gat gct 5687 Leu His Gly Ser Tyr Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala 1845 1850 1855 ctt gtc gac cta act gga gga gct ggt gag gag att gac ttg cgg agt 5735 Leu Val Asp Leu Thr Gly Gly Ala Gly Glu Glu Ile Asp Leu Arg Ser 1860 1865 1870 gct caa gca caa ata gat ctt gca agt ggc aga ttg tgg tct caa ttg 5783 Ala Gln Ala Gln Ile Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu 1875 1880 1885 tta cgt ttt aaa caa gag ggg ttc tta ctt ggt gct gga agt cca tca 5831 Leu Arg Phe Lys Gln Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser 1890 1895 1900 gga tct gat gtt cat gta tct tcc agt ggc att gtg caa ggg cat gct 5879 Gly Ser Asp Val His Val Ser Ser Ser Gly Ile Val Gln Gly His Ala 1905 1910 1915 1920 tac tcc gtc tta cag gtg aga gag gtt gat ggg cac aga ctt gtt cag 5927 Tyr Ser Val Leu Gln Val Arg Glu Val Asp Gly His Arg Leu Val Gln 1925 1930 1935 att cga aat cca tgg gct aat gaa gtt gag tgg aat ggt ccc tgg tca 5975 Ile Arg Asn Pro Trp Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser 1940 1945 1950 gac tca tcc cca gag tgg act gat agg atg aag cac aag ctg aag cat 6023 Asp Ser Ser Pro Glu Trp Thr Asp Arg Met Lys His Lys Leu Lys His 1955 1960 1965 gtt cca cag tca aaa gaa ggt ata ttc tgg atg tct tgg caa gat ttc 6071 Val Pro Gln Ser Lys Glu Gly Ile Phe Trp Met Ser Trp Gln Asp Phe 1970 1975 1980 cag att cat ttc aga tca ata tat gtg tgt cgg gtt tac ccc cgt gag 6119 Gln Ile His Phe Arg Ser Ile Tyr Val Cys Arg Val Tyr Pro Arg Glu 1985 1990 1995 2000 atg cgc tac tct gta aat ggc caa tgg cga ggt tat agt gcc ggt ggc 6167 Met Arg Tyr Ser Val Asn Gly Gln Trp Arg Gly Tyr Ser Ala Gly Gly 2005 2010 2015 tgc caa gat tat agc tca tgg cat caa aat cca caa ttc agg ctg agg 6215 Cys Gln Asp Tyr Ser Ser Trp His Gln Asn Pro Gln Phe Arg Leu Arg 2020 2025 2030 gca act ggt tct gat gca tct tta cca att cat gtg ttc atc acc tta 6263 Ala Thr Gly Ser Asp Ala Ser Leu Pro Ile His Val Phe Ile Thr Leu 2035 2040

2045 act cag ggc gta ggt ttc tcg aga aca act cct gga ttt cgt aac tac 6311 Thr Gln Gly Val Gly Phe Ser Arg Thr Thr Pro Gly Phe Arg Asn Tyr 2050 2055 2060 caa tca agc cat gat tca cag ttg ttc tat atc gga ttg agg att ctt 6359 Gln Ser Ser His Asp Ser Gln Leu Phe Tyr Ile Gly Leu Arg Ile Leu 2065 2070 2075 2080 aaa act cgt gga cgt cgt gct gct tac aac ata ttt ctt cat gaa tct 6407 Lys Thr Arg Gly Arg Arg Ala Ala Tyr Asn Ile Phe Leu His Glu Ser 2085 2090 2095 gtt ggt gga aca gac tat gtg aat tcc cgt gag att tca tgt gaa atg 6455 Val Gly Gly Thr Asp Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Met 2100 2105 2110 gtt ctt gac cct gat cct aag ggt tat act att gtc cca acc acg ata 6503 Val Leu Asp Pro Asp Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile 2115 2120 2125 cac cca ggg gaa gaa gca cct ttt gtc ctt tca gtc ttc aca aaa gca 6551 His Pro Gly Glu Glu Ala Pro Phe Val Leu Ser Val Phe Thr Lys Ala 2130 2135 2140 tcc att gtt ctt gaa gct t tgtagtgccc gtattgtcag atggctctct 6600 Ser Ile Val Leu Glu Ala 2145 2150 cagcaacctg catgccatga aatcatccaa gtgcttgcgt tgtttaagga accagacggc 6660 ttacgtctca atgttaagac ttgttttgcc cacgatccac gcaacattag agaagactta 6720 actccctctg caacagcgtc tgatctgata gctggccatc acattattgg tgaatctatg 6780 aagtctcggg ataaaatgtt ttagttagtg tcctgatttt gaaccacgaa gttaactgct 6840 cagaggatag ataatttgca ctggagaaag cacttttcag gcatggctca ccggcattcc 6900 atctcgatct tgagacacgc atcaaatgta catttgtagg tattgcgaca agtacagatt 6960 tattaaatgt agaataacta cttcataact gaggaaacaa ggagagacat aggagacttg 7020 tgacttgttg ggtaaaggtt tggtattcgg tagtgatagc tgcagttttg gttggtgtgt 7080 tgtaatattc agac 7094 27 2150 PRT Arabidopsis thaliana 27 Met Glu Gly Asp Glu Arg Gly Val Leu Leu Ala Cys Val Ile Ser Gly 1 5 10 15 Thr Leu Phe Thr Val Phe Gly Ser Gly Ser Phe Trp Ile Leu Trp Ala 20 25 30 Val Asn Trp Arg Pro Trp Arg Leu Tyr Ser Trp Ile Phe Ala Arg Lys 35 40 45 Trp Pro Lys Val Leu Gln Gly Pro Gln Leu Asp Ile Leu Cys Gly Val 50 55 60 Leu Ser Leu Phe Ala Trp Ile Val Val Val Ser Pro Ile Ala Ile Leu 65 70 75 80 Ile Gly Trp Gly Ser Trp Leu Ile Val Ile Leu Asp Arg His Ile Ile 85 90 95 Gly Leu Ala Ile Ile Met Ala Gly Thr Ala Leu Leu Leu Ala Phe Tyr 100 105 110 Ser Ile Met Leu Trp Trp Arg Thr Gln Trp Gln Ser Ser Arg Ala Val 115 120 125 Ala Leu Leu Leu Leu Leu Gly Val Ala Leu Leu Cys Ala Tyr Glu Leu 130 135 140 Cys Ala Val Tyr Val Thr Ala Gly Ala His Ala Ser Gln Gln Tyr Ser 145 150 155 160 Pro Ser Gly Phe Phe Phe Gly Val Ser Ala Ile Ala Leu Ala Ile Asn 165 170 175 Met Leu Phe Ile Cys Arg Met Val Phe Asn Gly Asn Gly Leu Asp Val 180 185 190 Asp Glu Tyr Val Arg Arg Ala Tyr Lys Phe Ala Tyr Ser Asp Cys Ile 195 200 205 Glu Val Gly Pro Val Ala Cys Leu Pro Glu Pro Pro Asp Pro Asn Glu 210 215 220 Leu Tyr Pro Arg Gln Thr Ser Arg Ala Ser His Leu Gly Leu Leu Tyr 225 230 235 240 Leu Gly Ser Leu Val Val Leu Leu Ala Tyr Ser Val Leu Tyr Gly Leu 245 250 255 Thr Ala Arg Glu Ser Arg Trp Leu Gly Gly Ile Thr Ser Ala Ala Val 260 265 270 Ile Val Leu Asp Trp Asn Ile Gly Ala Cys Leu Tyr Gly Phe Lys Leu 275 280 285 Leu Gln Asn Arg Val Leu Ala Leu Phe Val Ala Gly Ile Ser Arg Leu 290 295 300 Phe Leu Ile Cys Phe Gly Ile His Tyr Trp Tyr Leu Gly His Cys Ile 305 310 315 320 Ser Tyr Ile Phe Val Ala Ser Val Leu Ser Gly Ala Ala Val Ser Arg 325 330 335 His Leu Ser Ile Thr Asp Pro Ser Ala Ala Arg Arg Asp Ala Leu Gln 340 345 350 Ser Thr Val Ile Arg Leu Arg Glu Gly Phe Arg Arg Lys Glu Gln Asn 355 360 365 Ser Ser Ser Ser Ser Ser Asp Gly Cys Gly Ser Ser Ile Lys Arg Ser 370 375 380 Ser Ser Ile Asp Ala Gly His Thr Gly Cys Thr Asn Glu Ala Asn Arg 385 390 395 400 Thr Ala Glu Ser Cys Thr Ala Asp Asn Leu Thr Arg Thr Gly Ser Ser 405 410 415 Gln Glu Gly Ile Asn Ser Asp Lys Ser Glu Glu Ser Gly Arg Pro Ser 420 425 430 Leu Gly Leu Arg Ser Ser Ser Cys Arg Ser Val Val Gln Glu Pro Glu 435 440 445 Ala Gly Thr Ser Tyr Phe Met Asp Lys Val Ser Asp Gln Asn Asn Thr 450 455 460 Leu Val Val Cys Ser Ser Ser Gly Leu Asp Ser Gln Gly Tyr Glu Ser 465 470 475 480 Ser Thr Ser Asn Ser Ala Asn Gln Gln Leu Leu Asp Met Asn Leu Ala 485 490 495 Leu Ala Phe Gln Asp Gln Leu Asn Asn Pro Arg Ile Ala Ser Ile Leu 500 505 510 Lys Lys Lys Ala Lys Glu Gly Asp Leu Glu Leu Thr Asn Leu Leu Gln 515 520 525 Asp Lys Gly Leu Asp Pro Asn Phe Ala Val Met Leu Lys Glu Lys Asn 530 535 540 Leu Asp Pro Thr Ile Leu Ala Leu Leu Gln Arg Ser Ser Leu Asp Ala 545 550 555 560 Asp Arg Asp His Arg Asp Asn Thr Asp Ile Thr Ile Ile Asp Ser Asn 565 570 575 Ser Val Asp Asn Thr Leu Pro Asn Gln Ile Ser Leu Ser Glu Glu Leu 580 585 590 Arg Leu Arg Gly Leu Glu Lys Trp Leu Lys Leu Ser Arg Leu Leu Leu 595 600 605 His His Val Ala Gly Thr Pro Glu Arg Ala Trp Gly Leu Phe Ser Leu 610 615 620 Val Phe Ile Leu Glu Thr Ile Ile Val Ala Ile Phe Arg Pro Lys Thr 625 630 635 640 Ile Thr Ile Ile Asn Ser Ser His Gln Gln Phe Glu Phe Gly Phe Ser 645 650 655 Val Leu Leu Leu Ser Pro Val Val Cys Ser Ile Met Ala Phe Leu Arg 660 665 670 Ser Leu Gln Val Glu Glu Met Ala Leu Thr Ser Lys Ser Arg Lys Tyr 675 680 685 Gly Phe Val Ala Trp Leu Leu Ser Thr Ser Val Gly Leu Ser Leu Ser 690 695 700 Phe Leu Ser Lys Ser Ser Val Leu Leu Gly Ile Ser Leu Thr Val Pro 705 710 715 720 Leu Met Ala Ala Cys Leu Ser Ile Ala Val Pro Ile Trp Met His Asn 725 730 735 Gly Tyr Gln Phe Trp Val Pro Gln Leu Ser Cys Gly Asp Gln Ala Arg 740 745 750 Asp Leu Arg Ser Pro Arg Ile Lys Gly Phe Ile Leu Trp Ile Cys Val 755 760 765 Val Leu Phe Ala Gly Ser Val Ile Ser Leu Gly Ala Ile Ile Ser Ala 770 775 780 Lys Pro Leu Asp Asp Leu Lys Tyr Lys Leu Phe Ser Ala Arg Glu Asn 785 790 795 800 Asn Val Thr Ser Pro Tyr Thr Ser Ser Val Tyr Leu Gly Trp Ala Met 805 810 815 Ser Ser Gly Ile Ala Leu Val Val Thr Ala Ile Leu Pro Ile Val Ser 820 825 830 Trp Phe Ala Thr Tyr Arg Phe Ser His Ser Ser Ala Val Cys Leu Met 835 840 845 Ile Phe Ser Val Val Leu Val Ala Phe Cys Gly Thr Ser Tyr Leu Glu 850 855 860 Val Val Lys Ser Arg Asp Asp Gln Leu Pro Thr Lys Gly Asp Phe Leu 865 870 875 880 Ala Ala Leu Leu Pro Leu Ala Cys Ile Pro Ala Leu Leu Ser Leu Cys 885 890 895 Cys Gly Met Val Lys Trp Lys Asp Asp Cys Trp Ile Leu Ser Arg Gly 900 905 910 Val Tyr Val Phe Phe Ser Ile Gly Leu Leu Leu Leu Phe Gly Ala Ile 915 920 925 Ala Ala Val Ile Ala Val Lys Pro Trp Thr Ile Gly Val Ser Phe Leu 930 935 940 Leu Val Leu Phe Leu Met Val Val Thr Ile Gly Val Ile His Leu Trp 945 950 955 960 Ala Ser Asn Asn Phe Tyr Leu Thr Arg Lys Gln Thr Ser Phe Val Cys 965 970 975 Phe Leu Ala Leu Leu Leu Gly Leu Ala Ala Phe Leu Leu Gly Trp His 980 985 990 Gln Asp Lys Ala Phe Ala Gly Ala Ser Val Gly Tyr Phe Thr Phe Leu 995 1000 1005 Ser Leu Leu Ala Gly Arg Ala Leu Ala Val Leu Leu Ser Pro Pro Ile 1010 1015 1020 Val Val Tyr Ser Pro Arg Val Leu Pro Val Tyr Val Tyr Asp Ala His 1025 1030 1035 1040 Ala Asp Cys Gly Lys Asn Val Ser Ala Ala Phe Leu Val Leu Tyr Gly 1045 1050 1055 Ile Ala Leu Ala Thr Glu Gly Trp Gly Val Val Ala Ser Leu Ile Ile 1060 1065 1070 Tyr Pro Pro Phe Ala Gly Ala Ala Val Ser Ala Ile Thr Leu Val Val 1075 1080 1085 Ala Phe Gly Phe Ala Val Ser Arg Pro Cys Leu Thr Leu Glu Met Met 1090 1095 1100 Glu Val Ala Val Arg Phe Leu Ser Lys Asp Thr Ile Val Gln Ala Ile 1105 1110 1115 1120 Ser Arg Ser Ala Thr Lys Thr Arg Asn Ala Leu Ser Gly Thr Tyr Ser 1125 1130 1135 Ala Pro Gln Arg Ser Ala Ser Ser Ala Ala Leu Leu Val Gly Asp Pro 1140 1145 1150 Ser Ala Met Arg Asp Lys Ala Gly Asn Phe Val Leu Pro Arg Asp Asp 1155 1160 1165 Val Met Lys Leu Arg Asp Arg Leu Arg Asn Glu Glu Arg Val Ala Gly 1170 1175 1180 Ser Ile Phe Tyr Lys Met Gln Cys Arg Lys Gly Phe Arg His Glu Pro 1185 1190 1195 1200 Pro Thr Asn Val Asp Tyr Arg Arg Asp Met Cys Ala His Ala Arg Val 1205 1210 1215 Leu Ala Leu Glu Glu Ala Ile Asp Thr Glu Trp Val Tyr Met Trp Asp 1220 1225 1230 Lys Phe Gly Gly Tyr Leu Leu Leu Leu Leu Gly Leu Thr Ala Lys Ala 1235 1240 1245 Glu Arg Val Gln Asp Glu Val Arg Leu Arg Leu Phe Leu Asp Ser Ile 1250 1255 1260 Gly Phe Ser Asp Leu Ser Ala Arg Lys Ile Ser Lys Trp Lys Pro Glu 1265 1270 1275 1280 Asp Arg Arg Gln Phe Glu Ile Ile Gln Glu Ser Tyr Leu Arg Glu Lys 1285 1290 1295 Glu Met Glu Glu Glu Ser Leu Met Gln Arg Arg Glu Glu Glu Gly Arg 1300 1305 1310 Gly Lys Glu Arg Arg Lys Ala Leu Leu Glu Lys Glu Glu Arg Lys Trp 1315 1320 1325 Lys Glu Ile Glu Ala Ser Leu Ile Pro Ser Ile Pro Asn Ala Gly Ser 1330 1335 1340 Arg Glu Ala Ala Ala Met Ala Ala Ala Ile Arg Ala Val Gly Gly Asp 1345 1350 1355 1360 Ser Val Leu Glu Asp Ser Phe Ala Arg Glu Arg Val Ser Gly Ile Ala 1365 1370 1375 Arg Arg Ile Arg Thr Ala Gln Leu Glu Arg Arg Ala Gln Gln Thr Gly 1380 1385 1390 Ile Ser Gly Ala Val Cys Val Leu Asp Asp Glu Pro Met Ile Ser Gly 1395 1400 1405 Lys His Cys Gly Gln Met Asp Ser Ser Val Cys Gln Ser Gln Lys Ile 1410 1415 1420 Ser Phe Ser Val Thr Ala Met Ile Gln Ser Asp Ser Gly Pro Val Cys 1425 1430 1435 1440 Leu Phe Gly Thr Glu Phe Gln Lys Lys Val Cys Trp Glu Ile Leu Val 1445 1450 1455 Ala Gly Ser Glu Gln Gly Ile Glu Ala Gly Gln Val Gly Leu Arg Leu 1460 1465 1470 Ile Thr Lys Gly Glu Arg Gln Thr Thr Val Ala Arg Glu Trp Tyr Ile 1475 1480 1485 Gly Ala Thr Ser Ile Thr Asp Gly Arg Trp His Thr Val Thr Ile Thr 1490 1495 1500 Ile Asp Ala Asp Ala Gly Glu Ala Thr Cys Tyr Ile Asp Gly Gly Phe 1505 1510 1515 1520 Asp Gly Tyr Gln Asn Gly Leu Pro Leu Ser Ile Gly Ser Ala Ile Trp 1525 1530 1535 Glu Gln Gly Ala Glu Val Trp Leu Gly Val Arg Pro Pro Ile Asp Val 1540 1545 1550 Asp Ala Phe Gly Arg Ser Asp Ser Asp Gly Val Glu Ser Lys Met His 1555 1560 1565 Ile Met Asp Val Phe Leu Trp Gly Lys Cys Leu Ser Glu Glu Glu Ala 1570 1575 1580 Ala Ser Leu His Ala Ala Ile Gly Met Ala Asp Leu Asp Met Ile Asp 1585 1590 1595 1600 Leu Ser Asp Asp Asn Trp Gln Trp Thr Asp Ser Pro Pro Arg Val Asp 1605 1610 1615 Gly Trp Asp Ser Asp Pro Ala Asp Val Asp Leu Tyr Asp Arg Asp Asp 1620 1625 1630 Val Asp Trp Asp Gly Gln Tyr Ser Ser Gly Arg Lys Arg Arg Ser Gly 1635 1640 1645 Arg Asp Phe Val Met Ser Val Asp Ser Phe Ala Arg Arg His Arg Lys 1650 1655 1660 Pro Arg Met Glu Thr Gln Glu Asp Ile Asn Gln Arg Met Arg Ser Val 1665 1670 1675 1680 Glu Leu Ala Val Lys Glu Ala Leu Ser Ala Arg Gly Asp Lys Gln Phe 1685 1690 1695 Thr Asp Gln Glu Phe Pro Pro Asn Asp Arg Ser Leu Phe Val Asp Thr 1700 1705 1710 Gln Asn Pro Pro Ser Lys Leu Gln Val Val Ser Glu Trp Met Arg Pro 1715 1720 1725 Asp Ser Ile Val Lys Glu Asn Gly Ser Asp Ser Arg Pro Cys Leu Phe 1730 1735 1740 Ser Gly Asp Ala Asn Pro Ser Asp Val Cys Gln Gly Arg Leu Gly Asp 1745 1750 1755 1760 Cys Trp Phe Leu Ser Ala Val Ala Val Leu Thr Glu Val Ser Arg Ile 1765 1770 1775 Ser Glu Val Ile Ile Thr Pro Glu Tyr Asn Glu Glu Gly Ile Tyr Thr 1780 1785 1790 Val Arg Phe Cys Ile Gln Gly Glu Trp Val Pro Val Val Ile Asp Asp 1795 1800 1805 Trp Ile Pro Cys Glu Ser Pro Gly Lys Pro Ala Phe Ala Thr Ser Arg 1810 1815 1820 Lys Leu Asn Glu Leu Trp Val Ser Met Val Glu Lys Ala Tyr Ala Lys 1825 1830 1835 1840 Leu His Gly Ser Tyr Glu Ala Leu Glu Gly Gly Leu Val Gln Asp Ala 1845 1850 1855 Leu Val Asp Leu Thr Gly Gly Ala Gly Glu Glu Ile Asp Leu Arg Ser 1860 1865 1870 Ala Gln Ala Gln Ile Asp Leu Ala Ser Gly Arg Leu Trp Ser Gln Leu 1875 1880 1885 Leu Arg Phe Lys Gln Glu Gly Phe Leu Leu Gly Ala Gly Ser Pro Ser 1890 1895 1900 Gly Ser Asp Val His Val Ser Ser Ser Gly Ile Val Gln Gly His Ala 1905 1910 1915 1920 Tyr Ser Val Leu Gln Val Arg Glu Val Asp Gly His Arg Leu Val Gln 1925 1930 1935 Ile Arg Asn Pro Trp Ala Asn Glu Val Glu Trp Asn Gly Pro Trp Ser 1940 1945 1950 Asp Ser Ser Pro Glu Trp Thr Asp Arg Met Lys His Lys Leu Lys His 1955 1960 1965 Val Pro Gln Ser Lys Glu Gly Ile Phe Trp Met Ser Trp Gln Asp Phe 1970 1975 1980 Gln Ile His Phe Arg Ser Ile Tyr Val Cys Arg Val Tyr Pro Arg Glu 1985 1990 1995 2000 Met Arg Tyr Ser Val Asn Gly Gln Trp Arg Gly Tyr Ser Ala Gly Gly 2005 2010 2015 Cys Gln Asp Tyr Ser Ser Trp His Gln Asn Pro Gln Phe Arg Leu Arg 2020 2025 2030 Ala Thr Gly Ser Asp Ala Ser Leu Pro Ile His Val Phe Ile Thr Leu 2035 2040 2045 Thr Gln Gly Val Gly Phe Ser Arg Thr Thr Pro Gly Phe Arg Asn Tyr 2050 2055 2060 Gln Ser Ser His Asp Ser Gln Leu Phe Tyr Ile Gly Leu Arg Ile Leu 2065 2070 2075 2080 Lys Thr Arg Gly Arg Arg Ala Ala Tyr Asn Ile Phe Leu His Glu Ser 2085 2090 2095 Val Gly Gly Thr Asp Tyr Val Asn Ser Arg Glu Ile Ser Cys Glu Met 2100 2105 2110 Val Leu Asp Pro Asp Pro Lys Gly Tyr Thr Ile Val Pro Thr Thr Ile 2115 2120 2125 His Pro Gly Glu Glu Ala Pro Phe Val Leu Ser Val Phe Thr Lys Ala 2130 2135 2140 Ser Ile Val Leu Glu Ala 2145 2150 28 20035 DNA Arabidopsis thaliana CDS (4001)...(14037) genomic DNA sequence 28 tgggaggagt ggtgcatctt

agcacggcga agttagctag aggtttctta gtgtgtttat 60 gtctttgggg ttttttctcg ctatcatacg ccgcaaggtc cggcgtatca aagcagaagt 120 ttgaagtgaa gaaacatttg aacaggctga acaaacctgc tgtcaaaagc attcaggtat 180 atatattttt tttttagtgt tttggtttct tctatttgga ttggtttttg atagacaaat 240 gagagatctt tagaacatta tcataatctt atgattcact tatttctctc tccttcctgc 300 tacacataat gattcgattt acttttattt tattaacgat tccataaaag ttatggtcat 360 aattaaggat tttgacttga aatgttaatg ttactttgtt gttgaacaga gttcagatgg 420 tgatgtgatt gactgtgttc caatctcaaa gcaaccagct tttgatcatc cgttcctcaa 480 agatcacaag attcaggtct agagaataaa aacataactg atttagatct ggctttgtgt 540 tttatgttgt aagtgctgat gtttttatat atactttgtt tttttttaga tgaagcctaa 600 ttaccaccct gaaggactct ttgatgacaa caaagtgtct gctcctaaat caaatgagaa 660 agaagggcat attcctcagt tgtggcatcg atatggtaaa tgttctgaag gaactattcc 720 catgaggagg acaaaggaag atgatgtttt gagagcaagt tcagttaaaa gatatggcaa 780 gaagaagcgt agaagtgtcc ctttacctaa atctgcagaa cctgacctta ttaaccaaag 840 tggtcaccag gtgagactaa gtgtgaatgt gttgtgaatc ttttttattt ttgttggttt 900 ctggatctga gatgttttgt tttggtttca tgaattgtag catgccatag cttatgtaga 960 aggagataag tactatggag ctaaggctac tataaatgtg tgggagccaa agatacagca 1020 gcagaatgag ttcagcttgt cacagatatg gcttcttggt ggctcattcg gacaagatct 1080 taatagcatc gaagctggtt ggcaggtact acaatgttat cccaacgaga aacatataag 1140 aaatgtgaat aagatttgtg tgtgattctt ttttcatgtt tagaatattg actcttgttg 1200 cttttattta caggtgagcc cggatctgta tggtgacaat aacacgagac tcttcactta 1260 ctggactgta agatcctaac actcccacct aatcaagttc ttactgtcat atctttctgt 1320 ttctgcttat atttctctgt tgtatcttac gttaccaaat tatttgcaga gtgatgcata 1380 tcaagctacc ggttgctaca atcttctttg ctccggtttt attcaaatca acagtgacat 1440 agcaatggga gcaagcattt ccccggtctc tggatatcgt aactcgcagt acgatatcag 1500 tattctgatc tggaaggtat gttaagccat aaagagataa gtttgacgca atccataaga 1560 caagaaaagc tgaaatggtc tgttaacttt tttcacagga tcctaaagag ggacactggt 1620 ggatgcaatt tgggaacggc tatgttttag gctattggcc atcttttctc ttctcctact 1680 tgacagaaag tgcatcgatg attgaatggg gaggagaagt cgtgaactca caatcagatg 1740 gccagcacac ttcaacacaa atgggcagtg gtaaatttcc agaagaaggc tttagcaaag 1800 caagttactt caggaacatt caggtggttg atgggtcaaa caacctcaag gcacctaaag 1860 gacttggaac attcactgaa cagtctaact gttatgatgt tcaaaccgga agcaatgatg 1920 attggggtca ttacttttac tatggaggcc ctggtaaaaa ccagaagtgt ccataagcta 1980 ggctccaagt agtagtggct cgtctctcga gttatcttgt cttgtgaatt caccttgttt 2040 ctttctacac acactcactc aaatccgcct tcttgggtag tttttaggga tggttaaagt 2100 tatgtaatgg taaaagtttg gtgtcttttt ctccatgtaa gtaggtgggg aagaagtgga 2160 aaggtgtggc ttaagtttct ttgaaaactc agaaactttg gcccttagtg taattttact 2220 ttgtctcctt tgtattgtga tttgtttaaa caagtgaatg aatctctctt tctcactctt 2280 attaacaaat aatttcaggg aattaatatt aacttttgcc ttaatctctt tataaaccta 2340 ctctctaatg cccttttttg acaattagag attttacttt gttttctcat caccttttgg 2400 taaatttgac ttcacattta gagagagaga gagtaaaatg atctaaaaat ggtcatggat 2460 tgcatttgaa aattgtattt ggtgatgact gatttcatga actgtctctc tttggatgat 2520 gatcttttgc aaatgggtga atcatattta ttttttccac ttttttcaca ataaagttcc 2580 aagcatatac aattatgaag tacacatata ctgaatttac aacaaaactt ttcctacaac 2640 cttctaattt taaatatgct attttcttaa gtgaaaaagt taaatattaa tattgtagat 2700 ctgccttttg aaaataaata tggtcttgta tgttcagtag actatctttg acttaatcgg 2760 cttaaatttg ggattaaaga aattagaaac aagaaaacaa tttaaaaatt ctaatttgta 2820 ttatatcata tagttgatga tttaaatttt gtgacagaga cattggtctt ccacttcttt 2880 tttcctggtt atgagctttg agcctataac ctgaatttga cttacttaaa cttatttagt 2940 aattatacta tttattcatg aattaggtac tattttatca ttcttgtctt cttctttttt 3000 ctttcctacc ataatactat ttttattttc ttttaatcac acatatcaca atattatact 3060 tctgttttgt tttgtgcatt taacactttt tgtctaaatt aatattggat tggataaaat 3120 attgagtgta ataagataac atataaattc aggtaataaa aaccaattaa aaacagatgt 3180 aagtcttttt ttgacaaatc ttaatttagc taaatccgta tctaaatcat tccattctca 3240 ctgaccaata aatactcaga gcgtgtacac cacttgatat ctcattggct catggttcac 3300 acgcgcgtgt agcgtttgtt cggacagaac catagcttta tttattttat taaattaatt 3360 gtgttttttt ttccacaaga actttttctc tctctacaat acgtcgtctc tttgaggaag 3420 agagagagtg agaaaaaggt agtagcttct tttttttagt ttacgggttc acacattcaa 3480 agggtagggt tttaatctta ccttctctct ctatccattt cccaaaacct gagaaacaaa 3540 tttctctgag atttattcaa aaaaaaatca acattttgca gaaagcaaaa gcaagaaaac 3600 cctaaaagga agctaatttg tgtttcacaa gctcttcaga gtgtgagccc tatctttctg 3660 ttttctctac tttacagctt gattcttctg attttaaacc ctagtgaacc aaatctgtat 3720 ccaagatgtc tcattttgct tgaaggctgc taaagtttcg atttttaagc gatccctttc 3780 tgcttcgatg ccactgcaca gtaagctcat ttcggtcaag taggtaaggt ttctaaactt 3840 aagctgggtt ttgtgaaatt taggtttggt ttttgtgttc ttggttggtt ttaagctgcg 3900 gatttgatga tttgtgcgca agcttggggt ttcagctttt ttttttgtga tggaattttg 3960 atttccgagt tgcatggtgt tgtaggtggg agaagaagcc atg gaa ggg gat gag 4015 Met Glu Gly Asp Glu 1 5 cga gga gtc tta ctt gct tgt gta att tcg ggt acc ctt ttc acg gtt 4063 Arg Gly Val Leu Leu Ala Cys Val Ile Ser Gly Thr Leu Phe Thr Val 10 15 20 ttc ggt tcg ggt tcg ttt tgg ata ctt tgg gct gtt aat tgg cgg cca 4111 Phe Gly Ser Gly Ser Phe Trp Ile Leu Trp Ala Val Asn Trp Arg Pro 25 30 35 tgg cgt ctc tac agg taa aca agt cca ttc ata tgt ttg ggc agc tct 4159 Trp Arg Leu Tyr Arg * Thr Ser Pro Phe Ile Cys Leu Gly Ser Ser 40 45 50 agg tgt tgt ttt gtc ata tat ggc gct tga tag agt tgc ttt gta tga 4207 Arg Cys Cys Phe Val Ile Tyr Gly Ala * * Ser Cys Phe Val * 55 60 65 gta gta ttg aca tag aac cat agt tag tga atg att ttg gaa tgt gtt 4255 Val Val Leu Thr * Asn His Ser * * Met Ile Leu Glu Cys Val 70 75 cta ttt ttg cag ttg gat ctt tgc tag aaa atg gcc aaa agt att gca 4303 Leu Phe Leu Gln Leu Asp Leu Cys * Lys Met Ala Lys Ser Ile Ala 80 85 90 agg tcc tca gct tga tat act atg tgg tgt tct atc tct ttt tgc ttg 4351 Arg Ser Ser Ala * Tyr Thr Met Trp Cys Ser Ile Ser Phe Cys Leu 95 100 105 gat tgt ggt agt atc ccc tat tgc aat ctt gat agg atg ggg ttc ttg 4399 Asp Cys Gly Ser Ile Pro Tyr Cys Asn Leu Asp Arg Met Gly Phe Leu 110 115 120 gct gat tgt gat att gga tcg aca tat cat tgg gct ggc gat aat aat 4447 Ala Asp Cys Asp Ile Gly Ser Thr Tyr His Trp Ala Gly Asp Asn Asn 125 130 135 140 ggc tgg aac agc cct ttt act ggc att cta ctc aat cat gct ttg gtg 4495 Gly Trp Asn Ser Pro Phe Thr Gly Ile Leu Leu Asn His Ala Leu Val 145 150 155 gag gac cca gtg gca aag ctc aag tat gtt tac cat cta ctc ttt ctg 4543 Glu Asp Pro Val Ala Lys Leu Lys Tyr Val Tyr His Leu Leu Phe Leu 160 165 170 act taa ttc aca aca ttc ttc tgg tgt tat att ctt gat aat agt ggt 4591 Thr * Phe Thr Thr Phe Phe Trp Cys Tyr Ile Leu Asp Asn Ser Gly 175 180 185 ggc acg tag tgt tag tgt atg aag tat ccg atg tag gaa tgg gaa caa 4639 Gly Thr * Cys * Cys Met Lys Tyr Pro Met * Glu Trp Glu Gln 190 195 200 agc aga aca tac ctg aca cat cac tac tta ttg tga atg aac tat tga 4687 Ser Arg Thr Tyr Leu Thr His His Tyr Leu Leu * Met Asn Tyr * 205 210 act tct ttc tta cat tct cgt ttg aac aaa cag gag ctg tcg ctt tac 4735 Thr Ser Phe Leu His Ser Arg Leu Asn Lys Gln Glu Leu Ser Leu Tyr 215 220 225 230 ttc tcc ttc ttg gtg ttg cct tac tat gtg cgt atg aac tct gtg ctg 4783 Phe Ser Phe Leu Val Leu Pro Tyr Tyr Val Arg Met Asn Ser Val Leu 235 240 245 tct atg tta cgg ctg gtg cgc atg cat ctc agc aat att ctc ctt ctg 4831 Ser Met Leu Arg Leu Val Arg Met His Leu Ser Asn Ile Leu Leu Leu 250 255 260 gtt tct ttt tcg gtg tat cag caa tcg cgt tgg caa tta aca tgc tat 4879 Val Ser Phe Ser Val Tyr Gln Gln Ser Arg Trp Gln Leu Thr Cys Tyr 265 270 275 tta tct gcc gca tgg tct tta atg gta aag ttt acc cag tcc tta atc 4927 Leu Ser Ala Ala Trp Ser Leu Met Val Lys Phe Thr Gln Ser Leu Ile 280 285 290 aac atg caa ttg ctg tgt att caa ctt tat cag tct act taa ttc ttt 4975 Asn Met Gln Leu Leu Cys Ile Gln Leu Tyr Gln Ser Thr * Phe Phe 295 300 305 gta tta gct caa agc aaa tct tcc gta tct att aac atg cat act ttt 5023 Val Leu Ala Gln Ser Lys Ser Ser Val Ser Ile Asn Met His Thr Phe 310 315 320 325 taa cct cca acc tca att taa gtt gtt gaa act ttg cga aat ttt cca 5071 * Pro Pro Thr Ser Ile * Val Val Glu Thr Leu Arg Asn Phe Pro 330 335 tca ccg cag gaa atg gtt tag atg tgg acg aat atg taa gga ggg cat 5119 Ser Pro Gln Glu Met Val * Met Trp Thr Asn Met * Gly Gly His 340 345 350 ata aat ttg ctt att cag att gta tag aag tgg gtc ctg tgg ctt gtt 5167 Ile Asn Leu Leu Ile Gln Ile Val * Lys Trp Val Leu Trp Leu Val 355 360 365 tgc ctg aac ctc ctg atc cta atg aat tat atc ccc ggc aaa cca gca 5215 Cys Leu Asn Leu Leu Ile Leu Met Asn Tyr Ile Pro Gly Lys Pro Ala 370 375 380 ggt aag ttc ttg ttg ctc tct acc tag aaa ttc aat aat att ttg gca 5263 Gly Lys Phe Leu Leu Leu Ser Thr * Lys Phe Asn Asn Ile Leu Ala 385 390 395 tag ctg gcc tct tat gtt atg att gtt cct gaa ttt tgc aat caa caa 5311 * Leu Ala Ser Tyr Val Met Ile Val Pro Glu Phe Cys Asn Gln Gln 400 405 410 act att gaa tac ttg gaa cca acc taa atc tga aca gga ttc agg gct 5359 Thr Ile Glu Tyr Leu Glu Pro Thr * Ile * Thr Gly Phe Arg Ala 415 420 425 agt aat ctt gcc gtt tct ctc tag ctt ttt ttt ttt tcg ccc aac agg 5407 Ser Asn Leu Ala Val Ser Leu * Leu Phe Phe Phe Ser Pro Asn Arg 430 435 440 tgg tgt gga tgg tgt tat gat ttg ttt ata ctg agc gct gaa atg ttc 5455 Trp Cys Gly Trp Cys Tyr Asp Leu Phe Ile Leu Ser Ala Glu Met Phe 445 450 455 tca ttc ata ttt tta tca atc agc tat aat ctt ttt tct tgc ata tat 5503 Ser Phe Ile Phe Leu Ser Ile Ser Tyr Asn Leu Phe Ser Cys Ile Tyr 460 465 470 475 gta ggg ctt cac atc ttg gcc ttc tgt acc tgg gct cac tcg tag ttc 5551 Val Gly Leu His Ile Leu Ala Phe Cys Thr Trp Ala His Ser * Phe 480 485 490 tcc ttg cct act cag tcc tat atg gtc tca cag cta ggg aat cac gtt 5599 Ser Leu Pro Thr Gln Ser Tyr Met Val Ser Gln Leu Gly Asn His Val 495 500 505 ggc ttg gag gaa tca cat cag ctg cag tta ttg ttc ttg gta aaa aga 5647 Gly Leu Glu Glu Ser His Gln Leu Gln Leu Leu Phe Leu Val Lys Arg 510 515 520 taa aaa tta ttt act tat att taa agt tga agc agg cta ctt gat tat 5695 * Lys Leu Phe Thr Tyr Ile * Ser * Ser Arg Leu Leu Asp Tyr 525 530 535 tcc tca act cat ctt cat gct taa tat ttt cat gta gac tgg aat att 5743 Ser Ser Thr His Leu His Ala * Tyr Phe His Val Asp Trp Asn Ile 540 545 550 ggg gca tgc ttg tat ggg ttt aag ctt ctt cag aat cgt gtt ctg gca 5791 Gly Ala Cys Leu Tyr Gly Phe Lys Leu Leu Gln Asn Arg Val Leu Ala 555 560 565 ctt ttt gtt gct ggc ata tcc cgt ctt ttc cta ata tgt ttt ggc ata 5839 Leu Phe Val Ala Gly Ile Ser Arg Leu Phe Leu Ile Cys Phe Gly Ile 570 575 580 cac tac tgg ttt gtt tct gag cta aaa agc ttg tta ctt atc tat aga 5887 His Tyr Trp Phe Val Ser Glu Leu Lys Ser Leu Leu Leu Ile Tyr Arg 585 590 595 tct aaa gtt tgt ccc ctt cat ggc atg taa gat att atc cgt tct gtt 5935 Ser Lys Val Cys Pro Leu His Gly Met * Asp Ile Ile Arg Ser Val 600 605 610 gtt gca ggt acc tag ggc att gta tta gtt aca ttt tcg tag cat cag 5983 Val Ala Gly Thr * Gly Ile Val Leu Val Thr Phe Ser * His Gln 615 620 625 ttc tat cag gtg ctg ctg ttt ctc ggc atc tat cta taa cag acc cat 6031 Phe Tyr Gln Val Leu Leu Phe Leu Gly Ile Tyr Leu * Gln Thr His 630 635 640 cag ctg caa gaa gag atg cct tac aga gca cag tga tcc gct tga gag 6079 Gln Leu Gln Glu Glu Met Pro Tyr Arg Ala Gln * Ser Ala * Glu 645 650 655 aag gtt ttc gga gaa aag agc aga ata gtt ctt caa gtt ctt cag atg 6127 Lys Val Phe Gly Glu Lys Ser Arg Ile Val Leu Gln Val Leu Gln Met 660 665 670 gtt gtg gct caa gta taa aaa gaa gta gta gta tcg atg ctg gcc ata 6175 Val Val Ala Gln Val * Lys Glu Val Val Val Ser Met Leu Ala Ile 675 680 685 ctg gtt gta cta atg aag caa atc gta cgg cag aat ctt gca cgg ctg 6223 Leu Val Val Leu Met Lys Gln Ile Val Arg Gln Asn Leu Ala Arg Leu 690 695 700 aca atc taa ctc gaa cag gca gct ctc agg agg gaa tca ata gcg aca 6271 Thr Ile * Leu Glu Gln Ala Ala Leu Arg Arg Glu Ser Ile Ala Thr 705 710 715 aaa gcg aag aaa gtg gaa gac caa gct tag gtt tac gta gta gtt cat 6319 Lys Ala Lys Lys Val Glu Asp Gln Ala * Val Tyr Val Val Val His 720 725 730 gtc gtt ctg tgg tcc aag agc ccg aag cag gaa cgt ctt att tta tgg 6367 Val Val Leu Trp Ser Lys Ser Pro Lys Gln Glu Arg Leu Ile Leu Trp 735 740 745 aca aag ttt ctg atc aaa ata aca ctc ttg ttg ttt gtt cga gca gtg 6415 Thr Lys Phe Leu Ile Lys Ile Thr Leu Leu Leu Phe Val Arg Ala Val 750 755 760 765 gtc tag ata gcc aag gtt acg agt cta gca cat cga att ctg caa acc 6463 Val * Ile Ala Lys Val Thr Ser Leu Ala His Arg Ile Leu Gln Thr 770 775 780 agc agc ttt tgg ata tga att tgg ctc ttg ctt tcc agg acc agt taa 6511 Ser Ser Phe Trp Ile * Ile Trp Leu Leu Leu Ser Arg Thr Ser * 785 790 aca atc cta gga tag cct cga tac tta aga aga aag caa aag aag gtg 6559 Thr Ile Leu Gly * Pro Arg Tyr Leu Arg Arg Lys Gln Lys Lys Val 795 800 805 atc ttg aac tga cta att tgc tgc aag aca agg ggt tgg acc cta act 6607 Ile Leu Asn * Leu Ile Cys Cys Lys Thr Arg Gly Trp Thr Leu Thr 810 815 820 ttg ctg taa tgt tga agg aaa aaa act tgg atc cta cta tat tgg cac 6655 Leu Leu * Cys * Arg Lys Lys Thr Trp Ile Leu Leu Tyr Trp His 825 830 835 tac ttc aga gga gta gtt tgg atg cag ata gag atc acc gcg aca ata 6703 Tyr Phe Arg Gly Val Val Trp Met Gln Ile Glu Ile Thr Ala Thr Ile 840 845 850 ctg ata tta caa tca ttg act caa aca gtg ttg aca ata ctt tgc caa 6751 Leu Ile Leu Gln Ser Leu Thr Gln Thr Val Leu Thr Ile Leu Cys Gln 855 860 865 870 atc aga ttt ctt tat ccg aag aat tga gac tcc gtg gac tag aga agt 6799 Ile Arg Phe Leu Tyr Pro Lys Asn * Asp Ser Val Asp * Arg Ser 875 880 ggc tta agt tgt cta gac ttc ttc tgc acc atg tag cgg gga cac cag 6847 Gly Leu Ser Cys Leu Asp Phe Phe Cys Thr Met * Arg Gly His Gln 885 890 895 aga gag cat ggg gcc tct tta gtc ttg tct tta tcc ttg aaa caa tca 6895 Arg Glu His Gly Ala Ser Leu Val Leu Ser Leu Ser Leu Lys Gln Ser 900 905 910 915 ttg tgg cca ttt ttc gcc caa aga cca tca cga tta taa att cta gtc 6943 Leu Trp Pro Phe Phe Ala Gln Arg Pro Ser Arg Leu * Ile Leu Val 920 925 930 atc aac agg tga gtt cca ccc tct gat tta ttg tct ctg tgg aaa aaa 6991 Ile Asn Arg * Val Pro Pro Ser Asp Leu Leu Ser Leu Trp Lys Lys 935 940 945 ttt tgg gtc ttg ttt tgg ctt caa att act gcc agt tta ctc aaa ttg 7039 Phe Trp Val Leu Phe Trp Leu Gln Ile Thr Ala Ser Leu Leu Lys Leu 950 955 960 atc ctt gta gtt taa atg aag gtt att cgt acc ttt gct aac tca ctt 7087 Ile Leu Val Val * Met Lys Val Ile Arg Thr Phe Ala Asn Ser Leu 965 970 975 gtt atc taa ttg tag ttc gaa ttt ggt ttc tct gtg ctg cta ttg tca 7135 Val Ile * Leu * Phe Glu Phe Gly Phe Ser Val Leu Leu Leu Ser 980 985 990 cct gtt gtc tgt tca ata atg gct ttt ctt cgg tct ctt caa gtt gag 7183 Pro Val Val Cys Ser Ile Met Ala Phe Leu Arg Ser Leu Gln Val Glu 995 1000 1005 gaa atg gcc ttg aca tca aaa tct cgc aag gta tgg ctc gtc tct ttg 7231 Glu Met Ala Leu Thr Ser Lys Ser Arg Lys Val Trp Leu Val Ser Leu 1010 1015 1020 gtt tag tat gtt cat tag tac ttg ttg tgg aca atg cat ata ccc tgt 7279 Val * Tyr Val His * Tyr Leu Leu Trp Thr Met His Ile Pro Cys 1025 1030 1035 gat gtt tta att gtt cta aat tat gtt atg ttt cta tgt ttt cag tat 7327 Asp Val Leu Ile Val Leu Asn Tyr Val Met Phe Leu Cys Phe Gln Tyr 1040 1045 1050 ggc ttt gtt gcc tgg ctt ctg agc aca tca gtt gga ttg tca ctc tcg 7375 Gly Phe Val

Ala Trp Leu Leu Ser Thr Ser Val Gly Leu Ser Leu Ser 1055 1060 1065 ttc ttg agg tac tac gat ttt ttt atc ttc att gga cag cta cca act 7423 Phe Leu Arg Tyr Tyr Asp Phe Phe Ile Phe Ile Gly Gln Leu Pro Thr 1070 1075 1080 ttt cgc aca atg gtc aca gga tta tgt cat tca ttg gac agc tac caa 7471 Phe Arg Thr Met Val Thr Gly Leu Cys His Ser Leu Asp Ser Tyr Gln 1085 1090 1095 1100 ctt ttc tca ctt ggt cat ttt tat gtt ctc att aca aac atc atg cat 7519 Leu Phe Ser Leu Gly His Phe Tyr Val Leu Ile Thr Asn Ile Met His 1105 1110 1115 gct tca att ttg cag taa atc gtc agt act tct ggg aat atc ctt gac 7567 Ala Ser Ile Leu Gln * Ile Val Ser Thr Ser Gly Asn Ile Leu Asp 1120 1125 1130 tgt gcc cct cat ggc agc atg cct gtc tat tgc tgt tcc cat atg gat 7615 Cys Ala Pro His Gly Ser Met Pro Val Tyr Cys Cys Ser His Met Asp 1135 1140 1145 gca taa tgg gta cca att ttg ggt tcc aca gtt atc atg tgg tga cca 7663 Ala * Trp Val Pro Ile Leu Gly Ser Thr Val Ile Met Trp * Pro 1150 1155 1160 ggc aag aga ttt acg atc tcc cag gat aaa ggt ctg tgt gtt ttc cct 7711 Gly Lys Arg Phe Thr Ile Ser Gln Asp Lys Gly Leu Cys Val Phe Pro 1165 1170 1175 gac ctg gtt gca agt atc tct ctt tgt caa tta aat atc tct aac taa 7759 Asp Leu Val Ala Ser Ile Ser Leu Cys Gln Leu Asn Ile Ser Asn * 1180 1185 1190 act ttc ttt cca ttt tag ggg ttt att ctt tgg att tgt gtt gtg ttg 7807 Thr Phe Phe Pro Phe * Gly Phe Ile Leu Trp Ile Cys Val Val Leu 1195 1200 1205 ttt gcg ggt tct gta att tct ctt ggt gcg att ata tct gct aaa cct 7855 Phe Ala Gly Ser Val Ile Ser Leu Gly Ala Ile Ile Ser Ala Lys Pro 1210 1215 1220 ttg gat gat tta aag tat aag ctg ttt agt gcc aga gaa aac aac gtc 7903 Leu Asp Asp Leu Lys Tyr Lys Leu Phe Ser Ala Arg Glu Asn Asn Val 1225 1230 1235 acg tca cca tat aca tct tct gta tac ctt ggt tgg gca atg tca tct 7951 Thr Ser Pro Tyr Thr Ser Ser Val Tyr Leu Gly Trp Ala Met Ser Ser 1240 1245 1250 1255 gga att gct tta gta gtt acc gcc att cta cca ata gtt tca tgg ttt 7999 Gly Ile Ala Leu Val Val Thr Ala Ile Leu Pro Ile Val Ser Trp Phe 1260 1265 1270 gca act tat agg ttt tcc cac tct tct gct gtc tgt ctc atg ata ttc 8047 Ala Thr Tyr Arg Phe Ser His Ser Ser Ala Val Cys Leu Met Ile Phe 1275 1280 1285 tca ggt aat ttg tgt ttc tgt tag ttt gct cac att tgg ttg gga ttt 8095 Ser Gly Asn Leu Cys Phe Cys * Phe Ala His Ile Trp Leu Gly Phe 1290 1295 1300 ttt tcc ttc tcg aag tga tcc tta tat ttc ttt tcc tct act ttc tag 8143 Phe Ser Phe Ser Lys * Ser Leu Tyr Phe Phe Ser Ser Thr Phe * 1305 1310 1315 ttg ttc tcg tgg cat ttt gtg gaa ctt cat att tgg aag ttg taa aat 8191 Leu Phe Ser Trp His Phe Val Glu Leu His Ile Trp Lys Leu * Asn 1320 1325 1330 cta gag atg atc agt tgc cca caa agg gtg att tcc ttg cgg cct tgc 8239 Leu Glu Met Ile Ser Cys Pro Gln Arg Val Ile Ser Leu Arg Pro Cys 1335 1340 1345 ttc cac ttg cat gca ttc cgg cgc tgc ttt cac tat gct gtg gga tgg 8287 Phe His Leu His Ala Phe Arg Arg Cys Phe His Tyr Ala Val Gly Trp 1350 1355 1360 tta aat ggt gga tga cta ttt cct cac gtt gcc tat taa atc tta ctc 8335 Leu Asn Gly Gly * Leu Phe Pro His Val Ala Tyr * Ile Leu Leu 1365 1370 1375 tca gtt tca atg aga tcg tcc ttt taa atg ttt tgt ggt tgt tct ttg 8383 Ser Val Ser Met Arg Ser Ser Phe * Met Phe Cys Gly Cys Ser Leu 1380 1385 1390 cag gaa gga cga ttg ttg gat act ctc tcg agg tgt ata tgt ttt ctt 8431 Gln Glu Gly Arg Leu Leu Asp Thr Leu Ser Arg Cys Ile Cys Phe Leu 1395 1400 1405 ttc aat agg tct tct tct tct ttt tgg tgc gat agc agc tgt cat tgc 8479 Phe Asn Arg Ser Ser Ser Ser Phe Trp Cys Asp Ser Ser Cys His Cys 1410 1415 1420 agt caa acc atg gac ggt aaa tgc aaa ttg ctt aca taa ttc tca aag 8527 Ser Gln Thr Met Asp Gly Lys Cys Lys Leu Leu Thr * Phe Ser Lys 1425 1430 1435 ttt ttg gct tac ttt tct caa tct gat aca tct ttg tgt ttc aat aat 8575 Phe Leu Ala Tyr Phe Ser Gln Ser Asp Thr Ser Leu Cys Phe Asn Asn 1440 1445 1450 1455 aac aga tag gcg tat ctt ttc tct tag ttc ttt tcc tta tgg tgg taa 8623 Asn Arg * Ala Tyr Leu Phe Ser * Phe Phe Ser Leu Trp Trp * 1460 1465 caa ttg gtg taa tcc atc ttt ggg cgt caa aca att tct att taa cca 8671 Gln Leu Val * Ser Ile Phe Gly Arg Gln Thr Ile Ser Ile * Pro 1470 1475 1480 gga aac aga cat cct ttg tct gct ttc ttg ctc ttc ttt tgg gtt tgg 8719 Gly Asn Arg His Pro Leu Ser Ala Phe Leu Leu Phe Phe Trp Val Trp 1485 1490 1495 ccg cat tcc ttc tcg gat ggc atc aag gtg agg att gcc cat atc tta 8767 Pro His Ser Phe Ser Asp Gly Ile Lys Val Arg Ile Ala His Ile Leu 1500 1505 1510 ttt taa act ctt cgt aga ggt gag acc tac tct agt gac aca gca att 8815 Phe * Thr Leu Arg Arg Gly Glu Thr Tyr Ser Ser Asp Thr Ala Ile 1515 1520 1525 tca taa ttt ttt tgc aga taa agc att tgc tgg agc atc tgt tgg tta 8863 Ser * Phe Phe Cys Arg * Ser Ile Cys Trp Ser Ile Cys Trp Leu 1530 1535 1540 ctt tac att cct gtc tct gtt ggc tgg aag agc att agc tgt gag ttc 8911 Leu Tyr Ile Pro Val Ser Val Gly Trp Lys Ser Ile Ser Cys Glu Phe 1545 1550 1555 aac aga act gtg gct ttt tct gtt tct gta ttc aac cgg aat att aac 8959 Asn Arg Thr Val Ala Phe Ser Val Ser Val Phe Asn Arg Asn Ile Asn 1560 1565 1570 1575 gat tat tct cgt agg ttc ttc tat ccc cac caa ttg tag tat att ctc 9007 Asp Tyr Ser Arg Arg Phe Phe Tyr Pro His Gln Leu * Tyr Ile Leu 1580 1585 1590 caa ggg tgc tac cag tat atg tct acg atg ctc atg ctg att gcg gaa 9055 Gln Gly Cys Tyr Gln Tyr Met Ser Thr Met Leu Met Leu Ile Ala Glu 1595 1600 1605 aga atg tca ggt atg cag cca ctt gag tac ccc taa tag tat tgc atc 9103 Arg Met Ser Gly Met Gln Pro Leu Glu Tyr Pro * * Tyr Cys Ile 1610 1615 1620 tgc tga tct gaa atg acc ata aag gat taa gct gat cga ctc tca tat 9151 Cys * Ser Glu Met Thr Ile Lys Asp * Ala Asp Arg Leu Ser Tyr 1625 1630 gct ttt cta ttg att gca ttg gga ttt ctc tag tgc tgc att tct tgt 9199 Ala Phe Leu Leu Ile Ala Leu Gly Phe Leu * Cys Cys Ile Ser Cys 1635 1640 1645 cct gta tgg aat tgc ttt ggc aac aga agg ctg ggg tgt tgt tgc tag 9247 Pro Val Trp Asn Cys Phe Gly Asn Arg Arg Leu Gly Cys Cys Cys * 1650 1655 1660 tct gat aat tta tcc tcc gtt tgc ggg tgc tgc tgt atc agc tat cac 9295 Ser Asp Asn Leu Ser Ser Val Cys Gly Cys Cys Cys Ile Ser Tyr His 1665 1670 1675 1680 cct tgt agt agc ctt tgg gtt tgc tgt ttc tcg ccc atg ttt gac tct 9343 Pro Cys Ser Ser Leu Trp Val Cys Cys Phe Ser Pro Met Phe Asp Ser 1685 1690 1695 tga ggt tag ttt tct ggt tgt ttg att tat ttg ttc ctt aaa ata aag 9391 * Gly * Phe Ser Gly Cys Leu Ile Tyr Leu Phe Leu Lys Ile Lys 1700 1705 1710 att agt ggt tag tgg ttc att taa atc act tcg cta att cct tct gat 9439 Ile Ser Gly * Trp Phe Ile * Ile Thr Ser Leu Ile Pro Ser Asp 1715 1720 gtt ctt cag atg atg gag gtt gct gta cgc ttt ctt agc aag gat act 9487 Val Leu Gln Met Met Glu Val Ala Val Arg Phe Leu Ser Lys Asp Thr 1725 1730 1735 1740 ata gtg caa gct atc tct cga tct gcc acg aaa gta agt tac act ctg 9535 Ile Val Gln Ala Ile Ser Arg Ser Ala Thr Lys Val Ser Tyr Thr Leu 1745 1750 1755 atg tgt ctc ttg aaa atc ata ttg ata ttc tat tat tgg cct tat ttc 9583 Met Cys Leu Leu Lys Ile Ile Leu Ile Phe Tyr Tyr Trp Pro Tyr Phe 1760 1765 1770 aac aca ttc acg taa tat gga gct cca tgg gat gta tga ttt att ccg 9631 Asn Thr Phe Thr * Tyr Gly Ala Pro Trp Asp Val * Phe Ile Pro 1775 1780 1785 tct ttg caa ctt gtt aat tac aga caa gaa atg ctc tat ccg gca cgt 9679 Ser Leu Gln Leu Val Asn Tyr Arg Gln Glu Met Leu Tyr Pro Ala Arg 1790 1795 1800 att cag ctc ccc aaa ggt ccg cca gct ctg cag ctc ttc tgg ttg ggg 9727 Ile Gln Leu Pro Lys Gly Pro Pro Ala Leu Gln Leu Phe Trp Leu Gly 1805 1810 1815 atc cct ctg caa tgc gtg ata aag cag gga act ttg tgc ttc cta gag 9775 Ile Pro Leu Gln Cys Val Ile Lys Gln Gly Thr Leu Cys Phe Leu Glu 1820 1825 1830 atg atg tca tga aat taa ggg atc gtc tca gga acg aag aaa gag ttg 9823 Met Met Ser * Asn * Gly Ile Val Ser Gly Thr Lys Lys Glu Leu 1835 1840 1845 ctg gat caa tct tct aca aaa tgc aat gca gga aag gat tcc gtc atg 9871 Leu Asp Gln Ser Ser Thr Lys Cys Asn Ala Gly Lys Asp Ser Val Met 1850 1855 1860 aac cac cta caa atg tag att ata gaa gag aca tgt gtg ccc atg caa 9919 Asn His Leu Gln Met * Ile Ile Glu Glu Thr Cys Val Pro Met Gln 1865 1870 1875 gag ttt tgg cac tgg aag agg caa ttg ata cag aat ggg tgt ata tgt 9967 Glu Phe Trp His Trp Lys Arg Gln Leu Ile Gln Asn Gly Cys Ile Cys 1880 1885 1890 1895 ggg aca aat ttg gtg gtt att tac tac tat tgt tag gtt tga cag cta 10015 Gly Thr Asn Leu Val Val Ile Tyr Tyr Tyr Cys * Val * Gln Leu 1900 1905 agg cgg aga gag ttc agg tga atc ctg att cta aat ttt cat ata ttc 10063 Arg Arg Arg Glu Phe Arg * Ile Leu Ile Leu Asn Phe His Ile Phe 1910 1915 1920 ttc tct atg tat gtc taa cac ggg att att aca atg ttg tag gat gag 10111 Phe Ser Met Tyr Val * His Gly Ile Ile Thr Met Leu * Asp Glu 1925 1930 1935 gta cgg ttg cgg ctc ttc tta gat agc att ggg ttc tcg gat tta agt 10159 Val Arg Leu Arg Leu Phe Leu Asp Ser Ile Gly Phe Ser Asp Leu Ser 1940 1945 1950 gcc aga aaa atc agt aaa tgg aag cca gag gat aga aga caa ttc gaa 10207 Ala Arg Lys Ile Ser Lys Trp Lys Pro Glu Asp Arg Arg Gln Phe Glu 1955 1960 1965 1970 att att caa gag agg tat atg ttt ctt ata att atg tgg ttt gac gaa 10255 Ile Ile Gln Glu Arg Tyr Met Phe Leu Ile Ile Met Trp Phe Asp Glu 1975 1980 1985 gct gaa ctc tta gac tgt tgc cta tca ttt tct ttt gtg ttg taa att 10303 Ala Glu Leu Leu Asp Cys Cys Leu Ser Phe Ser Phe Val Leu * Ile 1990 1995 2000 tta gtt atc tga gag aga aag aga tgg aag agg aaa gcc tta tgc aga 10351 Leu Val Ile * Glu Arg Lys Arg Trp Lys Arg Lys Ala Leu Cys Arg 2005 2010 2015 gac gtg aag aag aag gga gag gta aag aaa gaa gga aag ctc ttt tgg 10399 Asp Val Lys Lys Lys Gly Glu Val Lys Lys Glu Gly Lys Leu Phe Trp 2020 2025 2030 aga agg aag agc gca aat gga agg aaa ttg aag cgt ccc tta ttc cat 10447 Arg Arg Lys Ser Ala Asn Gly Arg Lys Leu Lys Arg Pro Leu Phe His 2035 2040 2045 cta ttc cta atg ctg gta gca ggg agg cag cag cca tgg cag ctg caa 10495 Leu Phe Leu Met Leu Val Ala Gly Arg Gln Gln Pro Trp Gln Leu Gln 2050 2055 2060 tac gtg ctg ttg ggg gtg att ctg tcc ttg agg att cct tcg caa gag 10543 Tyr Val Leu Leu Gly Val Ile Leu Ser Leu Arg Ile Pro Ser Gln Glu 2065 2070 2075 2080 aga ggg tct cgg gta ttg cac gta gga tac gca ctg ctc aac tag aac 10591 Arg Gly Ser Arg Val Leu His Val Gly Tyr Ala Leu Leu Asn * Asn 2085 2090 2095 gac gtg cac aac agg tta aaa ctt att ttt ata ttc tgc aag tgt tct 10639 Asp Val His Asn Arg Leu Lys Leu Ile Phe Ile Phe Cys Lys Cys Ser 2100 2105 2110 ttc tta tga tgc tga tta atg gag aat taa cta aga aaa gtt att acc 10687 Phe Leu * Cys * Leu Met Glu Asn * Leu Arg Lys Val Ile Thr 2115 2120 aga ctg gaa tat ctg ggg cag ttt gtg ttc ttg atg atg aac caa tga 10735 Arg Leu Glu Tyr Leu Gly Gln Phe Val Phe Leu Met Met Asn Gln * 2125 2130 2135 taa gtg gta aac att gcg gcc aaa tgg act caa gtg tct gtc aaa gtc 10783 * Val Val Asn Ile Ala Ala Lys Trp Thr Gln Val Ser Val Lys Val 2140 2145 2150 aga aga tta gct ttt ccg tta cag caa tga tcc aat ccg att ctg gac 10831 Arg Arg Leu Ala Phe Pro Leu Gln Gln * Ser Asn Pro Ile Leu Asp 2155 2160 2165 ctg tat gtc ttt ttg gca ctg aat ttc aaa aga aag tat gtt ggg aga 10879 Leu Tyr Val Phe Leu Ala Leu Asn Phe Lys Arg Lys Tyr Val Gly Arg 2170 2175 2180 2185 ttc tgg ttg ctg gtt ctg agc aag gaa ttg agg ctg gcc aag ttg ggc 10927 Phe Trp Leu Leu Val Leu Ser Lys Glu Leu Arg Leu Ala Lys Leu Gly 2190 2195 2200 tta ggt tga taa caa aag gtg aga ggc aga caa ccg ttg cta gag agt 10975 Leu Gly * * Gln Lys Val Arg Gly Arg Gln Pro Leu Leu Glu Ser 2205 2210 2215 ggt ata ttg gtg caa cca gca taa ctg atg gaa ggt cta act ctt ttt 11023 Gly Ile Leu Val Gln Pro Ala * Leu Met Glu Gly Leu Thr Leu Phe 2220 2225 2230 ccc ctc agt ttc cat att gca aaa ttt act tcg agg act ttt atg atc 11071 Pro Leu Ser Phe His Ile Ala Lys Phe Thr Ser Arg Thr Phe Met Ile 2235 2240 2245 cat att tct cat gat ctg gtt tgg caa aag ttt ccg atg tta tca tgg 11119 His Ile Ser His Asp Leu Val Trp Gln Lys Phe Pro Met Leu Ser Trp 2250 2255 2260 att tct gtc ata ttt ata tat tag ctg ttt caa tca cag gtg gca tac 11167 Ile Ser Val Ile Phe Ile Tyr * Leu Phe Gln Ser Gln Val Ala Tyr 2265 2270 2275 agt gac aat cac aat tga tgc tga tgc ggg gga agc tac ttg tta cat 11215 Ser Asp Asn His Asn * Cys * Cys Gly Gly Ser Tyr Leu Leu His 2280 2285 2290 aga tgg tgg gtt tga tgg cta cca gaa tgg gtt acc tct aag tat tgg 11263 Arg Trp Trp Val * Trp Leu Pro Glu Trp Val Thr Ser Lys Tyr Trp 2295 2300 2305 cag tgc cat ttg gga aca agg agc tga agt ttg gtt ggg tgt tag gcc 11311 Gln Cys His Leu Gly Thr Arg Ser * Ser Leu Val Gly Cys * Ala 2310 2315 2320 acc tat aga tgt tga tgc att cgg gag atc aga tag tga tgg cgt cga 11359 Thr Tyr Arg Cys * Cys Ile Arg Glu Ile Arg * * Trp Arg Arg 2325 2330 atc aaa gat gca tat tat gga tgt ttt cct ttg ggg gaa atg ctt aag 11407 Ile Lys Asp Ala Tyr Tyr Gly Cys Phe Pro Leu Gly Glu Met Leu Lys 2335 2340 2345 tga aga aga ggc cgc ttc ttt gca tgc agc cat tgg cat ggc tga ctt 11455 * Arg Arg Gly Arg Phe Phe Ala Cys Ser His Trp His Gly * Leu 2350 2355 2360 aga cat gat tga ttt gtc tga tga caa ttg gca atg gac gga ttc acc 11503 Arg His Asp * Phe Val * * Gln Leu Ala Met Asp Gly Phe Thr 2365 2370 2375 ccc cag agt atg ttt tcc ttc tgt tga ctg ttg gca tat ttt ttc agt 11551 Pro Gln Ser Met Phe Ser Phe Cys * Leu Leu Ala Tyr Phe Phe Ser 2380 2385 2390 ccg atg ttt gtt aaa agg cta atg tta aaa cct att cta att ttt gtt 11599 Pro Met Phe Val Lys Arg Leu Met Leu Lys Pro Ile Leu Ile Phe Val 2395 2400 2405 gat tcc ttt ctc ttt tta cca ggt cga tgg ttg gga tag tga tcc tgc 11647 Asp Ser Phe Leu Phe Leu Pro Gly Arg Trp Leu Gly * * Ser Cys 2410 2415 2420 cga tgt tga tct cta tga tag gga tga cgt aga ttg gga tgg aca ata 11695 Arg Cys * Ser Leu * * Gly * Arg Arg Leu Gly Trp Thr Ile 2425 2430 ttc cag tgg gag gaa aag aag atc agg tcg gga ttt tgt aat gag tgt 11743 Phe Gln Trp Glu Glu Lys Lys Ile Arg Ser Gly Phe Cys Asn Glu Cys 2435 2440 2445 cga ttc ctt tgc cag gag aca cag gaa acc cag gat gga gac aca aga 11791 Arg Phe Leu Cys Gln Glu Thr Gln Glu Thr Gln Asp Gly Asp Thr Arg 2450 2455 2460 2465 aga tat aaa tca aag aat gcg ttc agt tga gtt ggc tgt caa aga agc 11839 Arg Tyr Lys Ser Lys Asn Ala Phe Ser * Val Gly Cys Gln Arg Ser 2470 2475 2480 tct ctc tgc acg agg tga taa gca att tac tga cca gga att tcc tcc 11887 Ser Leu Cys Thr Arg * * Ala Ile Tyr * Pro Gly Ile Ser Ser 2485 2490 aaa tga tcg ctc ttt att tgt

gga tac aca aaa tcc ccc atc aaa att 11935 Lys * Ser Leu Phe Ile Cys Gly Tyr Thr Lys Ser Pro Ile Lys Ile 2495 2500 2505 gca ggt atg gat tgt taa att ttt ctc att ttt tct gtt ttt agt taa 11983 Ala Gly Met Asp Cys * Ile Phe Leu Ile Phe Ser Val Phe Ser * 2510 2515 2520 ata tca ttt taa gtg att ata tat ctg gac aca aaa tct gcc ttc aca 12031 Ile Ser Phe * Val Ile Ile Tyr Leu Asp Thr Lys Ser Ala Phe Thr 2525 2530 2535 tag atc aag taa aac taa caa ctt gat ggg aac tct aac ctt ttg agt 12079 * Ile Lys * Asn * Gln Leu Asp Gly Asn Ser Asn Leu Leu Ser 2540 2545 2550 gtc ata att tca ggt tgt ttc tga atg gat gag acc tga ctc cat tgt 12127 Val Ile Ile Ser Gly Cys Phe * Met Asp Glu Thr * Leu His Cys 2555 2560 gaa aga aaa cgg tag tga ttc ccg tcc ctg cct gtt ctc tgg gga tgc 12175 Glu Arg Lys Arg * * Phe Pro Ser Leu Pro Val Leu Trp Gly Cys 2565 2570 2575 aaa tcc ttc aga tgt ttg cca ggt ttg atc act tac ttt ttc tgt tca 12223 Lys Ser Phe Arg Cys Leu Pro Gly Leu Ile Thr Tyr Phe Phe Cys Ser 2580 2585 2590 gtt act ttt tcc tga ccg tct tgt atg cat gtc aat tca aaa tga tcc 12271 Val Thr Phe Ser * Pro Ser Cys Met His Val Asn Ser Lys * Ser 2595 2600 2605 cag aga ttt att ctt gcg att ggg tta act tgt ttt ata ggg gcg ttt 12319 Gln Arg Phe Ile Leu Ala Ile Gly Leu Thr Cys Phe Ile Gly Ala Phe 2610 2615 2620 ggg gga ttg ttg gtt ctt aag cgc cgt tgc agt ttt gac aga ggt ttc 12367 Gly Gly Leu Leu Val Leu Lys Arg Arg Cys Ser Phe Asp Arg Gly Phe 2625 2630 2635 2640 acg aat atc tga agt gat cat tac tcc tga ata caa cga gga agg gat 12415 Thr Asn Ile * Ser Asp His Tyr Ser * Ile Gln Arg Gly Arg Asp 2645 2650 cta cac tgt tcg ttt ttg tat tca ggt tcc tgt ctc aaa cat tct ttc 12463 Leu His Cys Ser Phe Leu Tyr Ser Gly Ser Cys Leu Lys His Ser Phe 2655 2660 2665 2670 acg gca ctg ctt tgt tct ctt gat tgt agc atc gtg cag gtt ctt cta 12511 Thr Ala Leu Leu Cys Ser Leu Asp Cys Ser Ile Val Gln Val Leu Leu 2675 2680 2685 tga ttt gtg tac aat cag agg ttt gaa gtt tga ttg ctt tcc cct tgc 12559 * Phe Val Tyr Asn Gln Arg Phe Glu Val * Leu Leu Ser Pro Cys 2690 2695 2700 agg gtg agt ggg ttc ctg ttg tta tcg atg act gga ttc cat gtg aat 12607 Arg Val Ser Gly Phe Leu Leu Leu Ser Met Thr Gly Phe His Val Asn 2705 2710 2715 cac ctg gta aac cag ctt ttg cta cta gca gaa agc tca atg aac tct 12655 His Leu Val Asn Gln Leu Leu Leu Leu Ala Glu Ser Ser Met Asn Ser 2720 2725 2730 ggg tct cca tgg tgg aga aag cat atg cca agc tcc atg gtt ctt atg 12703 Gly Ser Pro Trp Trp Arg Lys His Met Pro Ser Ser Met Val Leu Met 2735 2740 2745 agg cac tgg agg ggg gac tgg ttc agg atg ctc ttg tcg acc taa ctg 12751 Arg His Trp Arg Gly Asp Trp Phe Arg Met Leu Leu Ser Thr * Leu 2750 2755 2760 gag gag ctg gtg agg aga ttg act tgc gga gtg ctc aag cac aaa tag 12799 Glu Glu Leu Val Arg Arg Leu Thr Cys Gly Val Leu Lys His Lys * 2765 2770 2775 atc ttg caa gtg gca gat tgt ggt ctc aat tgt tac gtt tta aac aag 12847 Ile Leu Gln Val Ala Asp Cys Gly Leu Asn Cys Tyr Val Leu Asn Lys 2780 2785 2790 agg ggt tct tac ttg gtg ctg gaa gtc cat cag gat ctg atg ttc atg 12895 Arg Gly Ser Tyr Leu Val Leu Glu Val His Gln Asp Leu Met Phe Met 2795 2800 2805 2810 tat ctt cca gtg gca ttg tgc aag ggc atg ctt act ccg tct tac agg 12943 Tyr Leu Pro Val Ala Leu Cys Lys Gly Met Leu Thr Pro Ser Tyr Arg 2815 2820 2825 tat tcc ctt ttg ctt tgg ttg cat ttg cca tat cct gag tat gag aac 12991 Tyr Ser Leu Leu Leu Trp Leu His Leu Pro Tyr Pro Glu Tyr Glu Asn 2830 2835 2840 aca att taa tat att tac gta ctg tct ttc ttc agg tga gag agg ttg 13039 Thr Ile * Tyr Ile Tyr Val Leu Ser Phe Phe Arg * Glu Arg Leu 2845 2850 2855 atg ggc aca gac ttg ttc aga ttc gaa atc cat ggg cta atg aag ttg 13087 Met Gly Thr Asp Leu Phe Arg Phe Glu Ile His Gly Leu Met Lys Leu 2860 2865 2870 agt gga atg gtc cct ggt cag act cat ccc cag agt gga ctg ata gga 13135 Ser Gly Met Val Pro Gly Gln Thr His Pro Gln Ser Gly Leu Ile Gly 2875 2880 2885 tga agc aca agc tga agc atg ttc cac agg tag ttt ctc ttg cgc tta 13183 * Ser Thr Ser * Ser Met Phe His Arg * Phe Leu Leu Arg Leu 2890 2895 2900 act tct tta cag ctc att cct tgt gtt cct tga att ctc tta gtc cga 13231 Thr Ser Leu Gln Leu Ile Pro Cys Val Pro * Ile Leu Leu Val Arg 2905 2910 2915 tta ggg gga cta tat ttc aga aac tgg taa aag att ttg ctt tat tgt 13279 Leu Gly Gly Leu Tyr Phe Arg Asn Trp * Lys Ile Leu Leu Tyr Cys 2920 2925 2930 taa caa ggg gac aca cat gga caa aac ata gtg ctc aca aac aca tag 13327 * Gln Gly Asp Thr His Gly Gln Asn Ile Val Leu Thr Asn Thr * 2935 2940 2945 tta ggt gta aat ata tgt aat tat gct atg gtt tct tca agt aaa gta 13375 Leu Gly Val Asn Ile Cys Asn Tyr Ala Met Val Ser Ser Ser Lys Val 2950 2955 2960 tat tgc gta ctg atc ctg tgc att ctg tta aac agt caa aag aag gta 13423 Tyr Cys Val Leu Ile Leu Cys Ile Leu Leu Asn Ser Gln Lys Lys Val 2965 2970 2975 tat tct gga tgt ctt ggc aag att tcc aga ttc att tca gat caa tat 13471 Tyr Ser Gly Cys Leu Gly Lys Ile Ser Arg Phe Ile Ser Asp Gln Tyr 2980 2985 2990 atg tgt gtc ggg ttt acc ccc gtg aga tgc gct act ctg taa atg gcc 13519 Met Cys Val Gly Phe Thr Pro Val Arg Cys Ala Thr Leu * Met Ala 2995 3000 3005 aat ggc gag gtt ata gtg ccg gtg gct gcc aag att ata gct cat ggc 13567 Asn Gly Glu Val Ile Val Pro Val Ala Ala Lys Ile Ile Ala His Gly 3010 3015 3020 atc aaa atc cac aat tca ggc tga ggg caa ctg gtt ctg atg cat ctt 13615 Ile Lys Ile His Asn Ser Gly * Gly Gln Leu Val Leu Met His Leu 3025 3030 3035 tac caa ttc atg tgt tca tca cct taa ctc agg cac atg cta tcc ctt 13663 Tyr Gln Phe Met Cys Ser Ser Pro * Leu Arg His Met Leu Ser Leu 3040 3045 3050 gat gga aac ttt ttc tat cta taa ttc ttt tgt aat tat cct cag ctc 13711 Asp Gly Asn Phe Phe Tyr Leu * Phe Phe Cys Asn Tyr Pro Gln Leu 3055 3060 3065 att ctc cca atg tta ctt aca ggg cgt agg ttt ctc gag aac aac tcc 13759 Ile Leu Pro Met Leu Leu Thr Gly Arg Arg Phe Leu Glu Asn Asn Ser 3070 3075 3080 3085 tgg att tcg taa cta cca atc aag cca tga ttc aca gtt gtt cta tat 13807 Trp Ile Ser * Leu Pro Ile Lys Pro * Phe Thr Val Val Leu Tyr 3090 3095 cgg att gag gat tct taa aac tcg tgg acg tcg tgc tgc tta caa cat 13855 Arg Ile Glu Asp Ser * Asn Ser Trp Thr Ser Cys Cys Leu Gln His 3100 3105 3110 att tct tca tga atc tgt tgg tgg aac aga cta tgt gaa ttc ccg tga 13903 Ile Ser Ser * Ile Cys Trp Trp Asn Arg Leu Cys Glu Phe Pro * 3115 3120 3125 gat ttc atg tga aat ggt tct tga ccc tga tcc taa ggg tta tac tat 13951 Asp Phe Met * Asn Gly Ser * Pro * Ser * Gly Leu Tyr Tyr 3130 3135 3140 tgt ccc aac cac gat aca ccc agg gga aga agc acc ttt tgt cct ttc 13999 Cys Pro Asn His Asp Thr Pro Arg Gly Arg Ser Thr Phe Cys Pro Phe 3145 3150 3155 agt ctt cac aaa agc atc cat tgt tct tga agc ttt gt agtgcccgta 14047 Ser Leu His Lys Ser Ile His Cys Ser * Ser Phe 3160 3165 ttgtcagatg gctctctcag caacctgcat gccatgaaat catccaagtg cttgtgttgt 14107 ttaaggaacc agacggctta cgtctcaatg ttaagacttg ttttgcccac gatccacgca 14167 acattagaga aggtaaattt cttaaccttt ctgcaaggat tgctcttttc ctatcctcac 14227 tcactgttcc tcattcagac ttaactccct ccgcaacagc gtctgatctg atagctggcc 14287 atcacattat tggtgaatct atgaagtctc gggataaaat gttttagtta ttgtcctgat 14347 tttgaaccac gaagttaact gctcagagga tagataattt gcactggaga aagcactttt 14407 caggcatggc tcaccggcat tccatctcga tcttgagaca cgcatcaaat gtacatttgt 14467 aggtattgcg acaagtacag atttattaaa tgtagaataa ctacttcata actgaggaaa 14527 caaggagaga aataggagac ttgtgacttg ttgggtaaag gtttggtatt cggtagtggt 14587 agctgcagtt ttggttggtg tgttgtaata ttcagacact aatctgtgtg cagatttcgt 14647 ttcaactcta atatatgagg gctctctttc tctctcgttc tctttcacta tctctcttaa 14707 tctgtgtttg gagttctctt tacactcgct cacccatttg taaatggaga ttctagaact 14767 tctcaacagg aaccgctgtt gtgtacatga attgtctatt tgcagatcaa ctagtataaa 14827 accaagcaaa aggtaagaaa agagacagat taagaagata ggttagcttt gaaagcaaaa 14887 gatccgtcaa aaatacaagt gcctcatagt taacctgtag taatgaagag aggcatctca 14947 aaatgcaacg aaaaagtgaa atacaccttc ctacccctcg ccaaaatctt gaatatctca 15007 tttaagttct ttcttcgacg gttcttcact ccgaatcgct ttccatgctc cccattgcct 15067 tcaatcctct tggtctctac atgaaaatat gaatgtatca cacctctctg ctagtacata 15127 aacattaact tctacagaga aactagaaag agaaaaatct gatagatacc ttctttgaag 15187 tcttcttctc tcttacttca tatctctctt tgcacagatc tgataaccaa gctcctggac 15247 tcaccagctg cattatacat aacaatcatg agataaaaat ggttatgaag aacacaaacg 15307 ttaagttttc aaagagactc acaagcaatg tcttctgaac caatttggct cgcttcttag 15367 gtctttgagg aagcttgcaa cctttcatag ccaaaaaatc ttcttccttc tctttatttg 15427 acaaagctat atacaacttt ggccacacat gcttctttgt ttctttcaca ggttccttaa 15487 taattttccc actctcatct attcccatcg aaccccttgt tgtataatac ctatcttcct 15547 tctcgggcga cgccataaac gatctccgca cattgactag agaatcagtg atcctatcac 15607 cgcagaaaca taacaatatc ttacatataa aaaaacttga aaaatatcac tttttccccc 15667 aataatactt acttgttagg acggttaaga tgtcgagaag gtgagcctct ctcagaagag 15727 acagcacgag aaatgagttt acgtttagtc aaacaatcag ttgatttgcc attggcaagg 15787 ctttgatctt tcttcacttt catacatcta actcttttcc tctcacccca ttgcaacaca 15847 aaatctgcct ctgtggttcc acttcctctt tgtcccttta cctcctccat tacacaacac 15907 tcactttctt tctcttaacc ttaaacaacc aacaaagaaa cataaactca gacattgaaa 15967 actcttccag aaatagacca aacaaatcta gaacaaccac taagaaactt ctgtttacaa 16027 aaggggaaaa agttctgttt acaaatcata ctcgtttctg atttctaaca aatactgaaa 16087 caaaaccaca gattaagata gacaaatcag gactaatttg tagagacaag acacactaac 16147 ctcaagatct gcaactttaa ctcagcgact tatcttccag aatcacccta caaagcagca 16207 atatccaaaa acaatattaa aaaaatcaga aaaaaaatag actaaaatct ataaatcaga 16267 tagtgtttac agcagaatcg attcttcaat actatataga acacaaacac accattgtca 16327 tataaataca gaaacaatag ctttaccaaa aaaataaacc caaaaaagaa gagcataaac 16387 acttacaaga gtgaaactag atgaaaccaa agacagaaac ttgggatgaa caatttttta 16447 ggtgaaatgg agaaaaaaag ttgttccctt ttcttctctc agaagatgga tagattcaaa 16507 ctccacaaaa aaatagaaaa agaatttgta atctggattt tcttttaata tctcgatgag 16567 accagaagat cgaaacactg tttatctatc ttctttaaac acacacacac acaaaagaaa 16627 taaaaactgc ttaccctctg gtctttactg cgacgctcac cttttttttt ttttttttta 16687 atatgccatt ttattatttt tgctcatttt aatttaataa tgaaaaaaat aatgtgattg 16747 ataacaaccg tcagatatgt aatggatggt gagatgggct tctagaagtt aaaaaaagcc 16807 taagcctgtt gtgtttgata aaagagtgaa ctcgttatgg ggactaacga atcatgttat 16867 gacatgtgta ttgtttttgt cgagtacacg ttagtacggt ttcagtggat aataaacacc 16927 atgccatatg tccggcgaga agatccaaaa taagattttg cgctaagaca gtaaaaaggt 16987 tttgtcccac gcgccaatga catctgattt ctcagatttg aacaaaattc gcctttggct 17047 tttacagggc ggtgagtgtc acgctctaaa gatgcgtgtg tgattagatg taaacccaat 17107 tcatattcaa acttgggcta atataggctt aatttatggg ctttcgtagt gaggcttttt 17167 acagaaattt tctaattttc tcatcattta tatagtttac ttactagtga gtatttagta 17227 acttgccctc taaaaagatt ttgtcagcaa ttttttggtg atactgctgc ttcgttttac 17287 aggtctaatg aatgcagcca aggtaataga ataatcgaat gagagctttg gtcatctgtc 17347 tggtaagatc atatccgttg gatgttcaat atgttgaggc ttttaagtct gaaacagaac 17407 ttgaagatta ggttacttgt tacttttaag ttgttaataa tgagattatc aagatctttt 17467 gggttatcga gttatgtcag tagagtcttc tactgttgta ccaatttgta acccttgtta 17527 tatatatata aaatgatatg ggaaaactta tagttttttt tttctctctt catttttgta 17587 cattcaagaa agaaaaaaga aaaaaataat aattttcttc gataataatc tccaattttt 17647 attttctata aaatttaata ttctcttttt tcaaataaat aaattcttgg ctataagtat 17707 ttctgtcaag ctaaatatat atatcaaatg aacaagtaga cattggactg ctagcttcac 17767 agagatgtac atagacgcag atatctgtaa accgtgtcta gatttcaaaa cactcttagc 17827 aaaatggaag tccagcatag taaaagagtt gtagttcttt tctgaaaaac aggtagaaat 17887 attatctctt aacctgattg agataaaaaa aacataatac ttaaatataa taagtatatt 17947 ctatattttt taaagtatat tatatttgtg tatcttgtaa ttgtaaaaga aatttgtatt 18007 ctgtcacgag tgaatatata tatgaatttt ttttcttaaa tgtaattgtg gttctatgca 18067 tatcatatat ttctgaaatt ccaaacggct atgtgttctc ttttgtggtc agtctcagtc 18127 acatttgttt atcttacaat aatttattat ttaaatgtgt tcctctaata agcagttaaa 18187 ggaggcaaat gagttttcaa aactgcaaaa tgagatttca aaacatattt ttgaagaaaa 18247 gttaactaga ttttaatcca accaatggtc cactagattt caaagttcca acatggaact 18307 cattattatg ctttggtcgg aaaagtgtta aagttttatt tttgaatctc actttctcgt 18367 tgcggccttg tttaaactac ttgctgatct aaaacttaaa cggttccttc taattcctta 18427 aacgacggag aatttaaatt agttaatgag tagtctttat gaaaaaatca aaataaaaga 18487 gagtgaataa ttcgaaaaca aaagtaacaa acaaataatt cgtatgttta cggtgttagg 18547 gtccacaaaa gcagtccagc ctcatgcata tgattatgca taattgtatg tttggtattt 18607 gtaagctttt ccagctattt tattttgtta tcttcttctt cggtctgttt gatttctttt 18667 tttgtatatg attttaaaaa gcatgctact gatatactaa ttcttttctt ttgtaattgg 18727 ggtagttggt cattttcata gtttggtgtg aacgttggta tgaacatcac taatttcaag 18787 gaaacactaa aacggctaat tgacccttta gtgcaatcag aatagtacat aaataaagta 18847 attgtttctt aaagaaaaaa aaattcttag atagaaggaa aaggagtatt ctagaatata 18907 gttttgatga aaaatagttc tccatataat aggtttattt ttaattaata aaatatatgt 18967 ttattgaaga aaaagaagga aatgatgctt agtaaatttg cttacaaact aagcaaaatc 19027 tctataaatt aatatttttg tgcgtaaaat attttattaa tttataaaga tattaactga 19087 taaaagttaa tctaagactt gatccacttt ataatctgtg gatccgcgag attactaagt 19147 ggattaaatt ctatgattat atttttctaa taacatatta atgtatttgt tataatttat 19207 gagtgtactc tccaaataat agtatatgat aaaaaatttt taatacacta aaaaaactat 19267 agaattttac ttagctcatc tcaatctcat ctagagtttg gttattaata gtactaagta 19327 atagctaatc ttttaaataa gtagaataaa aactttttaa atacaccaac aaagttagat 19387 gtaacttctt atcctttgaa caactcatgt gaagtcacaa atacaaaatt cattacaatc 19447 aaaacaaaaa ttagtaaaaa acgtttttgt gaggttggaa agaaaaaagg acagtggttg 19507 agaaaaagaa gaatgtggtt tgggccaaga gacagccaat ttgccagctg taaaatctca 19567 tacaattgtt gtataaatag acaataccat ccccacaact attatcatca caacacaaat 19627 caaaacaaga ataacaaaat ctttctctta taaattctta tttcaagaca tcaaaggaga 19687 attaatggag gcaatgaaga tgaagatgat ggtgtttatt atggttgttg cggtggcttt 19747 ctcagctgcc acggctgcta ccgttgaagc tccagctcca agcccaactt ctgatgctgc 19807 catgtttgta ccagcactgt ttgcatctgt tgttgctttg gcatctggtt tcatcttttg 19867 atcattcttt ttttcttcat tatttaattg catttgttaa tgagtttgcg tgatttgatt 19927 ctctattgtt agaatgttgc attcttattt cttaatgtaa ccatctgttt ctctatgtat 19987 ctatgtgtat tatcaattca tcactgttta tgagatcgtt tatcttaa 20035

Claims

1. An isolated polynucleotide comprising a member selected from the group consisting of: (a) a polynucleotide encoding a polypeptide having at least 80% sequence identity to SEQ ID NO:6; wherein the % sequence identity is determined over the entire length of the coding sequence by the GAP algorithm using default parameters and wherein expression of the polynucleotide alters the number or configuration of aleurone cells in a seed; (b) a polynucleotide which encodes a polypeptide of SEQ ID NO:6; (c) a polynucleotide comprising SEQ ID NO:5; and (d) a polynucleotide fragment which is a functional equivalent to a polynucleotide of (a) through (c ) wherein expression of the polynucleotide alters the number or configuration of aleurone cells in a seed.

2. An expression cassette comprising at least one polynucleotide of claim 1 operably linked to a promoter.

3. The expression cassette of claim 2 further comprising any combination of additional polynucleotide sequences of interest.

4. A transformed plant cell containing at least one polynucleotide of claim 1.

5. The transformed plant cell of claim 4, wherein the plant is corn, barley, soybean, sorghum, wheat, rice, alfalfa, safflower, sunflower, canola, cotton, or millet.

6. A transformed plant comprising at least one polynucleotide of claim 1.

7. The transformed plant of claim 6, wherein the plant is corn, barley, soybean, sorghum, wheat, rice, alfalfa, safflower, sunflower, canola, cotton, or millet.

8. A transformed seed from the transformed plant of claim 6.

9. A method for altering maize seed characteristics, the method comprising: (a) introducing into a maize plant cell the recombinant expression cassette of claim 2; (b) culturing the plant cell under plant forming conditions to produce a plant; and, (c) expressing the polynucleotide to alter the seed characteristics of the plant.

10. The method of claim 9 wherein the expression cassette further comprises any combination of additional polynucleotide sequences of interest.

11. The method of claim 10 wherein the additional polynucleotide is maize cr4 (crinkly 4).

12. A transgenic plant produced by the method of claim 9.

13. A transgenic seed produced by the transgenic plant of claim 12.

14. A method for increasing oil content of maize seeds, the method comprising: (a) introducing into a maize plant cell the recombinant expression cassette of claim 2; (b) culturing the plant cell under plant forming conditions to produce a plant; and, (c) expressing the polynucleotide to increase the oil content in the seeds of the plant.

15. The method of claim 14 wherein the expression cassette further comprises any combination of additional polynucleotide sequences of interest.

16. The method of claim 15 wherein the additional polynucleotide is maize cr4 (crinkly 4).

17. A transgenic plant produced by the method of claim 14.

18. A transgenic seed produced by the transgenic plant of claim 17.