U.S. patent application number 11/447664 was filed with the patent office on 2007-06-28 for endothelial cell-leukocyte adhesion molecules (elams) and molecules involved in leukocyte adhesion (milas).
This patent application is currently assigned to Biogen Inc., a Massachusetts corporation. Invention is credited to Christopher D. Benjamin, Susan E. Goelz, Catherine A. Hession, Roy R. Lobb, Laurelee Osborn, Margaret D. Rosa.
Application Number | 20070149769 11/447664 |
Document ID | / |
Family ID | 27407673 |
Filed Date | 2007-06-28 |
United States Patent
Application |
20070149769 |
Kind Code |
A1 |
Hession; Catherine A. ; et
al. |
June 28, 2007 |
Endothelial cell-leukocyte adhesion molecules (ELAMs) and molecules
involved in leukocyte adhesion (MILAs)
Abstract
Antibodies specific for ELAMs are disclosed.
Inventors: |
Hession; Catherine A.;
(Hingham, MA) ; Lobb; Roy R.; (Westwood, MA)
; Goelz; Susan E.; (Portland, OR) ; Osborn;
Laurelee; (Arlington, MA) ; Benjamin; Christopher
D.; (Beverly, MA) ; Rosa; Margaret D.;
(Gloucester, MA) |
Correspondence
Address: |
FISH & RICHARDSON
P.O. BOX 1022
MINNEAPOLIS
MN
55440-1022
US
|
Assignee: |
Biogen Inc., a Massachusetts
corporation
|
Family ID: |
27407673 |
Appl. No.: |
11/447664 |
Filed: |
June 6, 2006 |
Related U.S. Patent Documents
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10234041 |
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11447664 |
Jun 6, 2006 |
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08473764 |
Jun 7, 1995 |
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10234041 |
Sep 3, 2002 |
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08342642 |
Nov 21, 1994 |
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08473764 |
Jun 7, 1995 |
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07608298 |
Oct 31, 1990 |
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08342642 |
Nov 21, 1994 |
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07452675 |
Dec 18, 1989 |
5272263 |
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07608298 |
Oct 31, 1990 |
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07359516 |
Jun 1, 1989 |
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07452675 |
Dec 18, 1989 |
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07345151 |
Apr 28, 1989 |
5217870 |
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07359516 |
Jun 1, 1989 |
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Current U.S.
Class: |
530/388.22 |
Current CPC
Class: |
A61K 38/00 20130101;
C07K 2319/30 20130101; A61K 47/6849 20170801; A61P 29/00 20180101;
C07K 16/2836 20130101; C07K 16/28 20130101; A61P 35/00 20180101;
C07K 14/70564 20130101; C07K 16/4258 20130101; C12N 2310/12
20130101; A61P 17/00 20180101; C07K 16/4241 20130101; C12N 2310/11
20130101; A61P 1/00 20180101; C07K 2317/76 20130101; Y02A 50/30
20180101; C07K 16/2854 20130101; C12N 15/1138 20130101 |
Class at
Publication: |
530/388.22 |
International
Class: |
C07K 16/28 20060101
C07K016/28 |
Foreign Application Data
Date |
Code |
Application Number |
Apr 27, 1990 |
US |
PCT/US90/02357 |
Claims
1. An isolated antibody that specifically binds to VCAM1.
Description
TECHNICAL FIELD OF INVENTION
[0001] This invention relates to molecules involved in the adhesion
of leukocytes to endothelial cells during inflammation and to DNA
sequences that code on expression for them. More particularly, it
relates to Endothelial Cell Adhesion Molecules (ELAMs), including
ELAM1 and Vascular Cell Adhesion Molecule 1 and 1b (VCAM1 and
VCAM1b). It also relates to molecules on the surface of leukocytes
involved in leukocyte adhesion to endothelial cells (MILAs). These
include CDX, a molecule involved in the ELAM1 adhesion pathway, and
VLA4, the ligand of VCAM1 and VCAM1b. This invention also relates
to clone 7.2 and clone 1. These DNA sequences encode protein 7.2
and protein 1, respectively, which are involved in the expression
of CDX. These proteins appear to be 1,3-fucosyl transferases that
glycosylate CDX. This invention also relates to Pseudo-X and
Pseudo-X.sub.2, proteins that appear on COS 7 and CHO cells,
respectively, that have been transfected with clone 7.2. Cells
expressing those proteins bind ELAM1 and are recognized by anti-CDX
monoclonal antibodies. This invention further relates to antibodies
that recognize these adhesion molecules and anti-idiotype
antibodies that recognize both those antibodies and the ligands or
receptors for the adhesion molecules. The invention also relates to
antisense DNA and RNA molecules complementary to mRNA for such
adhesion molecules and also relates to ribozymes which recognize
mRNA for such molecules. The invention also relates to methods for
using the aforementioned molecules, DNA sequences, antibodies,
anti-idiotype antibodies, antisense molecules and ribozymes, for
example in developing diagnostic and therapeutic agents to detect
or inhibit leukocyte adhesion to endothelial cells.
BACKGROUND OF THE INVENTION
[0002] Inflammation is the response of vascularized tissues to
infection or injury. Clinically it is accompanied by four classic
signs: redness, heat, pain and swelling. Its course may be acute or
chronic.
[0003] At the cellular level, inflammation involves the adhesion of
leukocytes (white blood cells) to the endothelial wall of blood
vessels and their infiltration into the surrounding tissues.
(Harlan, 1985.) Acute inflammation is characterized by the adhesion
and infiltration of polymorphonuclear leukocytes (PMNs). (Harlan,
1987 and Malech and Gallin, 1987.) PMN accumulation in the tissues
reaches its peak between two and one half to four hours after an
inflammatory stimulus and ceases by about twenty-eight hours.
(Bevilacqua and Gimbrone, 1987.) In contrast, chronic inflammation
is characterized by the adhesion and infiltration of other
leukocytes, especially monocytes and lymphocytes.
[0004] In normal inflammation, the infiltrating leukocytes
phagocytize invading organisms or dead cells, and play a role in
tissue repair and the immune response. However, in pathologic
inflammation, infiltrating leukocytes can cause serious and
sometimes deadly damage. Rheumatoid arthritis and atherosclerosis
are examples of chronic inflammatory diseases in which mononuclear
leukocytes infiltrate the tissues and cause damage. (Hough and
Sokoloff, 1985 and Ross, 1986.) Multiple organ failure syndrome,
adult respiratory distress syndrome (ARDS), and ischemic
reperfusion injury are acute inflammations in which infiltrating
PMNs cause the damage (Harlan, 1987 and Malech and Gallin, 1987).
In multiple organ failure syndrome, which can occur after shock
such as that associated with severe burns, PMN-mediated damage
exacerbates the injury. In ARDS, PMNs cause the lungs to fill with
fluid, and the victim may drown. In ischemic reperfusion injury,
which occurs when tissue cut off from the supply of blood is
suddenly perfused with blood (for example after heart attack,
stroke, or limb re-attachment), PMN adhesion causes serious tissue
damage (Harlan, 1987).
[0005] Recognizing that leukocyte infiltration is the cause of much
inflammation-related pathology and that leukocyte adhesion is the
first step in infiltration, investigators have recently focused
attention on the mechanism of leukocyte binding to the endothelial
cell surface. Studies show that binding is mediated by cell-surface
molecules on both endothelial cells and leukocytes which act as
receptor and ligand (Harlan et al., 1987; Dana et al., 1986; and
Bevilacqua et al., 1987a).
[0006] During the course of inflammation, certain inflammatory
agents can act on the leukocytes, making them hyperadhesive for
endothelium. Known inflammatory agents include leukotriene-B4
(LTB4), complement factor 5a (C5a), and
formyl-methionyl-leucyl-phenylalanine (FMLP). These agents activate
a group of proteins called LeuCAMs. The LeuCAMs are dimers of the
CD11 and CD18 proteins. One of the LeuCAMs, CD11a/CD18 (also called
LFA1) binds to a receptor on endothelial cells called ICAM1
(intercellular adhesion molecule 1). (Harlan, 1985 and Dana et al.,
1986.) Investigators have shown that monoclonal antibodies (Moabs)
to LeuCAMs inhibit PMN adhesion to endothelium both in vitro and in
vivo. (Arfors, 1987; Vedder et al., 1988; and Todd, 1989.)
[0007] Other inflammatory agents act directly on endothelial cells
to substantially augment leukocyte adhesion. These agents include
the cytokines interleukin-1 (IL-1), lymphotoxin (LT) and tumor
necrosis factor (TNF), as well as the bacterial endotoxin,
lipopolysaccharide (LPS). For example, IL-1 has been shown to
stimulate adhesion of PMNs, monocytes, and the related cell lines
HL-60 (PMN-like) and U937 (monocyte-like), to human endothelial
cell monolayers. The action is both time-dependent and
protein-synthesis dependent. (Bevilacqua et al., 1987a; Bevilacqua
et al., 1987b; and Bevilacqua et al., 1985.)
[0008] Current evidence indicates that these agents induce a group
of molecules on the endothelial cell surface called ELAMs
(endothelial cell-leukocyte adhesion molecules). To date
investigators have identified two of these molecules, intercellular
adhesion molecule 1 (ICAM1) and endothelial cell-leukocyte adhesion
molecule 1 (ELAM1). (Simmons et al., 1988; Staunton et al., 1988;
and Bevilacqua et al., 1987b.) ICAM1 is found on many cell types,
and its expression on vascular endothelium is strongly upregulated
both in vitro and in vivo by the inflammatory cytokines
interleukin-1 (IL-1), tumor necrosis factor-.alpha. (TNF), and
gamma interferon (IFN-.gamma.). (Pober et al., 1986; Dustin and
Springer, 1988; and Cotran and Pober, 1988.)
[0009] ELAM1 was initially detected and characterized by a
monoclonal antibody that partially blocked PMN adhesion to
cytokine-treated human umbilical vein endothelial cells (HUVECs).
ELAM1 is a 116 kD cell surface glycoprotein rapidly synthesized by
HUVECs in response to the inflammatory cytokines IL-1 or TNF, but
not IFN-.gamma.. (Bevilacqua et al., 1987b.) Unlike ICAM1, ELAM1
appears to be expressed only in endothelium, and its expression is
transient even in the continued presence of cytokine. Like ICAM1,
ELAM1 is present at inflammatory sites in vivo. Immunohistologic
studies show that it exists at sites of acute, but not chronic,
inflammation and is absent from the non-inflamed vessel wall.
(Cotran et al., 1986 and Cotran and Pober, 1988.) Therefore, ELAM1
appears to be a major mediator of PMN adhesion to the inflamed
vascular wall in vivo. Importantly, the presence of ELAM1 on the
cell surface follows the natural course of acute inflammation,
appearing a few hours after stimulation and gradually dissipating
within a day. (Bevilacqua et al., 1987b.)
[0010] Indirect evidence suggests that other ELAMs exist. Although
inflammatory agents induce binding of PMNs, monocytes, and
lymphocytes to endothelium in vitro, Moabs against ELAM1 inhibit
only the binding of PMNs and related cells. (Bevilacqua and
Gimbrone, 1987.) Furthermore, maximal accumulation of lymphocytes
and monocytes at sites of inflammation in vivo occurs at about
twenty-four hours, when ELAM1 expression has returned to basal
levels. On the basis of such information investigators inferred the
presence of other ELAMs that mediate binding of these lymphocytes
and monocytes. (Bevilacqua et al., 1987b.) As set forth in detail
below, we have characterized and cloned two more ELAMS, designated
VCAM1 and VCAM1b, that mediate binding of lymphocytes to
endothelial cells. ELAMs accordingly may be regarded as a family of
molecules.
[0011] A growing body of evidence indicates that ELAMs may play
important roles in a wide range of pathological states involving
cell-cell recognition, including tumor invasion, metastasis and
viral infection. (Harlan, 1985; Wallis and Harlan, 1986; Bevilacqua
et al., 1987a; and Cotran and Pober, 1988.)
[0012] The adhesion of leukocytes to cells expressing ELAMs
suggests the existence on leukocytes of ELAM ligands. One such
molecule is the ICAM1 ligand, lymphocyte function associated
antigen 1 (LFA1). LFA1 is one of a trio of heterodimeric molecules
known as the 82 integrins or the CD11/18 family. (Dustin et al.,
1986; Rothlein et al., 1986; and Marlin and Springer, 1987.) Recent
studies show that the ICAM1/LFA1 pathway plays a role in both
lymphocyte and polymorphonuclear leukocyte (PMN) adhesion to
endothelial cells in vitro. (Dustin and Springer, 1988; Smith et
al., 1989.) We report here the isolation of a molecule involved in
leukocyte adhesion to endothelial cells (MILA) which may prove to
be an ELAM1 ligand. The molecule, designated CDX, is a protein of
approximately 150 kD and was isolated from HL-60 cells. Monoclonal
antibodies that recognize CDX inhibit the binding of PMNs and HL-60
cells to ELAM1-expressing cells. Furthermore, CDX is present on
leukocyte cell types known to adhere to ELAM1 and is absent from
leukocyte cell types and other cell types that do not adhere to
ELAM1. Thus, CDX is a molecule expressed on certain leukocytes that
plays an important role in ELAM1-mediated leukocyte-endothelial
cell adhesion. We also report the isolation and sequencing of cDNA
encoding molecules involved in CDX expression.
[0013] We also report the identification of a VCAM1 and VCAM1b
ligand, VLA4. (Hemler and Takada, EP 330 506). Antibodies specific
for the .alpha..sup.4 and .beta..sub.1 subunits of VLA4 completely
eliminate binding of VLA4-expressing cells to VCAM1.
[0014] Because leukocyte adhesion to the vascular wall is the first
step in inflammation, therapies directed to preventing this step
are attractive for the treatment of pathologic inflammation.
Clinicians are already testing, with some success, therapies based
on inhibiting leukocyte-mediated adhesion. One such approach
involves Moab binding to the leukocyte cell-surface complex,
CD11/CD18, to inhibit PMN adhesion. (Arfors et al., 1987; Vedder et
al., 1988; and Todd et al., 1989.)
[0015] We believe that alternative therapies for preventing
leukocyte adhesion, based on endothelial cell-mediated binding, and
on ELAMs and MILAs (including ELAM ligands), in particular, are
more promising. The ELAM system is particularly appealing for two
reasons: First, because ELAM expression on endothelial cells is
induced rather than constitutive, ELAMs are concentrated at sites
of inflammation and are limited in number. This means that adhesion
inhibitors need act only locally and, consequently, would be
effective at lower doses than inhibitors directed to constitutively
expressed molecules. Second, ELAM binding is selective for
different leukocyte classes. For example, ELAM1 binds PMNs, and
VCAM1 binds lymphocytes. Therefore, these therapies would be
specific for certain classes of leukocytes and would not affect the
circulation or migration of other leukocyte classes. Furthermore,
for the above reasons, such therapies may prove to be cheaper and
less toxic.
[0016] ELAM-based approaches to therapy require, as starting
materials, both ELAMs and MILAs in highly purified form, free of
normally associated animal proteins. There is also a need for
methods to produce these molecules. These and other needs have now
been met as described herein, by isolating DNA sequences that code
on expression for particular adhesion molecules and by constructing
recombinant DNA molecules and expression vehicles for their
production.
SUMMARY OF THE INVENTION
[0017] It is the principal object of this invention to provide new
means to study, diagnose, prevent and treat inflammation. More
particularly, it is an object of this invention to provide
molecules involved in leukocyte binding to endothelial cells and to
isolate other molecules which are themselves useful in inhibiting
the endothelial cell binding of leukocytes.
[0018] This invention provides DNA sequences that code on
expression for endothelial cell-leukocyte adhesion molecules
(ELAMs), genomic DNA sequences for ELAMs (including ELAM expression
control sequences), recombinant DNA molecules containing these DNA
sequences, unicellular hosts transformed with these DNA molecules,
processes for producing ELAMs, and ELAM proteins essentially free
of normally associated animal proteins. The present invention also
provides for antibody preparations reactive for ELAMs.
[0019] This invention also provides DNA sequences that code on
expression for molecules involved in leukocyte adhesion to
endothelial cells (MILAs). MILAs will include leukocyte surface
molecules that bind directly to ELAMs, i.e., ELAM ligands.
Monoclonal antibodies recognizing ELAM ligands can inhibit
ELAM/ELAM ligand binding directly. MILAs will also include
leukocyte surface molecules that are involved indirectly in
adhesion, for example molecules that inhibit ELAM/ELAM ligand
binding by interacting with a third molecule, such as a monoclonal
antibody. Such molecules may act, for example, by changing the
surface conformation of an ELAM ligand so that its affinity for the
ELAM is reduced. This invention also provides recombinant DNA
molecules containing MILA DNA sequences and unicellular hosts
transformed with them. It also provides for MILA proteins
essentially free of normally associated animal proteins, methods
for producing MILAs, and monoclonal antibodies that recognize
MILAs, particularly CDX.
[0020] This invention provides DNA sequences encoding molecules
that cause several cell lines, including COS, CHO and R1.1, both
express surface glycoproteins that are recognized by anti-CDX
(.alpha.-CDX) antibodies and to bind to ELAM1. This invention
provides, in particular, clone 7.2 and clone 1, and protein 7.2 and
protein 1, respectively. These proteins appear to be 1,3-fucosyl
transferases.
[0021] This invention also provides the glycoproteins, Pseudo-X and
Pseudo-X.sub.2, which cause COS cells and CHO cells, respectively,
to bind ELAM1 and to be recognized by .alpha.-CDX antibodies.
[0022] This invention further provides methods for inhibiting PMN
binding to endothelial cells involving the use of ELAMs, MILAs
including ELAM ligands, or portions of those molecules to block
receptors or ligands. It also relates to the use of antisense
nucleic acids and ribozymes to inhibit ELAM expression. The
invention also relates to methods for identifying binding
inhibitors by screening molecules for their ability to inhibit
binding of an ELAM to its ligand. It provides methods for
identifying ELAMs and their ligands. One such method involves using
anti-idiotypic antibodies against antibodies that recognize ELAMs
or ELAM ligands.
BRIEF DESCRIPTION OF THE DRAWINGS
[0023] FIG. 1 depicts the composite ELAM1 cDNA sequence and deduced
amino acid sequence derived from the DNA sequences of ELAM pCDM8
clone 6, pSQ148 and pSQ149. The nucleotides are numbered from 1 to
3863. Throughout this application we refer to the coding DNA
sequence of this figure as the DNA sequence for ELAM1. We also
refer to the molecule comprising the amino acid sequence depicted
in this figure as ELAM1.
[0024] FIG. 2 depicts the DNA sequence of the synthetic polylinker
of pNN11.
[0025] FIG. 3 depicts the sequence of cDNA coding for VCAM1 and the
deduced amino acid sequence of VCAM1 derived from AM pCDM8 clone
41. The nucleotides are numbered 1 to 2811. In this application we
refer to the coding DNA sequence of this figure as the DNA sequence
for VCAM1. We also refer to the molecule comprising the amino acid
sequence depicted in this figure as VCAM1.
[0026] FIG. 4 depicts the sequence of cDNA coding for VCAM1b and
the deduced amino acid sequence of VCAM1b derived from VCAM1b pCDM8
clone 1E11. The nucleotides are numbered 1 to 3080. In this
application we refer to the coding DNA sequence of this figure as
the DNA sequence for VCAM1b. We also refer to the molecule
comprising the amino acid sequence depicted in this figure as
VCAM1b.
[0027] FIG. 5 depicts the domain structure of VCAM1. The amino
acids are indicated according to the one letter code used by the
University of Wisconsin Genetics Computer Group. (Devereux et al.,
1984.)
[0028] FIG. 6 depicts the domain structure of VCAM1b. The amino
acids are indicated according to the one letter code used by the
University of Wisconsin Genetics Computer Group. (Devereux et al.,
1984.)
[0029] FIG. 7 depicts the DNA sequence of portions of the 5'
untranslated and untranscribed region of ELAM1 derived from clone
EL1-07.
[0030] FIG. 8 depicts the DNA sequence of portions of the 5'
untranslated and untranscribed region of VCAM1 derived from clone
VC1-16.
[0031] FIG. 9 depicts the sequence of cDNA coding for protein 7.2
and the deduced amino acid sequence of protein 7.2 derived from
pSQ219 and CDX pCDM8 clone 7.2. The nucleotides are numbered
1-2175. In this application we refer to the coding DNA sequence of
this figure as the DNA sequence for clone 7.2. We also refer to the
polypeptide comprising the amino acid sequence depicted in this
figure as protein 7.2.
[0032] FIG. 10 depicts the sequence of cDNA coding for protein 1
derived from clone 1. The nucleotides are numbered 1-2861. In this
application we refer to the coding DNA sequence of this figure as
the DNA sequence for clone 1. We also refer to the polypeptide
comprising the amino acid sequence depicted in this figure as
protein 1.
DETAILED DESCRIPTION OF THE INVENTION
[0033] In accordance with this detailed description, the following
definitions apply:
[0034] Expression control sequence--A DNA sequence that controls
and regulates the transcription and translation of another DNA
sequence.
[0035] Operatively linked--A DNA sequence is operatively linked to
an expression control sequence when the expression control sequence
controls and regulates the transcription and translation of that
DNA sequence. The term "operatively linked" includes having an
appropriate start signal (e.g., ATG) in front of the DNA sequence
to be expressed and maintaining the correct reading frame to permit
expression of the DNA sequence under the control of the expression
control sequence and production of the desired product encoded by
the DNA sequence. If a gene that one desires to insert into a
recombinant DNA molecule does not contain an appropriate start
signal, such a start signal can be inserted in front of the
gene.
[0036] Antibody--An immunoglobulin molecule or functional fragment
thereof, such as Fab, F(ab').sub.2 or dAb. An antibody preparation
is reactive for a particular antigen when at least a portion of the
individual immunoglobulin molecules in the preparation recognize
(i.e., bind to) the antigen. An antibody preparation is
non-reactive for an antigen when binding of the individual
immunoglobulin molecules in the preparation to the antigen is not
detectable by commonly used methods.
[0037] Standard hybridization conditions--salt and temperature
conditions substantially equivalent to 5.times.SSC and 65.degree.
C. for both hybridization and wash. Under standard hybridization
conditions the DNA sequences of this invention will hybridize to
other DNA sequences having sufficient homology, including
homologous sequences from different species. It is understood that
the stringency of hybridization conditions is a factor in the
degree of homology required for hybridization.
[0038] DNA sequences--The DNA sequences of this invention refer to
DNA sequences prepared or isolated using recombinant DNA
techniques. These include cDNA sequences, DNA sequences isolated
from their native genome, and synthetic DNA sequences. The term as
used in the claims is not intended to include naturally occurring
DNA sequences as they exist in Nature.
[0039] ELAM--A molecule expressed on the surface of endothelial
cells that mediates adhesion of leukocytes to endothelial
cells.
[0040] MILA--A molecule expressed on the surface of leukocytes that
is involved in ELAM-mediated binding to endothelial cells. This
includes ELAM ligands, i.e., molecules that bind directly to
ELAMs.
[0041] As described below, we have isolated and sequenced cDNAs
from ELAM mRNAs, expressed ELAM molecules in an appropriate host,
isolated and sequenced cDNAs encoding MILAs, and isolated and
expressed DNA sequences for MILAs.
[0042] Expression of recombinant DNA molecules according to this
invention may involve post-translational modification of a
resultant polypeptide by the host cell. For example, in mammalian
cells expression might include, among other things, glycosylation,
lipidation or phosphorylation of a polypeptide, or cleavage of a
signal sequence to produce a "mature" protein. Accordingly, as used
herein, the term "protein", including ELAM, MILA, protein 1,
protein 7.2, Pseudo-X and Pseudo-X.sub.2 encompass full-length
polypeptides and modifications or derivatives thereof, such as
glycosylated versions of such polypeptides, mature proteins,
polypeptides retaining a signal peptide, truncated polypeptides
having comparable biological activity, and the like.
[0043] ELAMs are expressed on the surface of endothelial cells only
during inflammation. We utilized this phenomenon to isolate ELAM
cDNAs. We have designated the polypeptides encoded by our cDNA
isolates ELAM1, VCAM1 and VCAM1b. The first step involved in the
isolation was selection of cells that differentially expressed the
ELAM molecules. We chose human umbilical vein endothelial cells
because they produce ELAMs when induced by the inflammatory
cytokine, IL-1.beta.. However, the practitioner is not limited to
this cytokine, to this cell type, or even to human cells in
particular. Other mammalian cells, e.g., baboon endothelial cells,
are also known to produce ELAMs. (Cotran and Pober, 1988.)
[0044] The next step was to isolate mRNA from cells expressing
ELAMS, in this case, IL-1.beta.-induced HUVECs, and to create a
cDNA library from them. Many methods are known for isolating mRNA
and for producing cDNA from it. (See, e.g., Gubler and Hoffman,
1983 and Maniatis et al., 1982.)
[0045] We then inserted the cDNA into an appropriate vector. We
chose the eukaryotic expression vector pCDM8, described by Brian
Seed. (Seed, 1987.) This plasmid has several advantages including a
high copy number in E. coli, a eukaryotic promoter, and high level
of expression in transient expression systems such as COS 7 cells.
However, several other vector systems are available. (See, e.g.,
Cate et al., 1986.)
[0046] After constructing a cDNA library, the next step was to
isolate from it clones containing ELAM cDNA sequences. There are
currently many ways to isolate cDNA for a differentially expressed
mRNA. These include, for example, (1) plus/minus screening with
labeled cDNA; (2) production of subtracted cDNA libraries; and (3)
screening with subtractive cDNA probes. (Davis, 1986; Sargent,
1987; Davis et al., 1985, Hedrick et al., 1984; and Duguid et al.,
1988.) We chose the third technique, screening with subtractive
cDNA probes, and produced a cDNA sublibrary enriched for ELAM
sequences.
[0047] As we will describe in more detail below, we produced a
subtractive cDNA probe enriched for mRNA produced by
cytokine-induced, but not uninduced cells. Then we probed the
cytokine-induced cDNA library with the subtracted cDNA probe using
techniques well known to the art. This enabled us to isolate clones
forming a sublibrary enriched for ELAM sequences.
[0048] At this point we used two techniques to identify clones that
contained cDNA for ELAM sequences. In a first method, we tested
clones for expression of ELAM activity in an appropriate eukaryotic
expression system. One can use a variety of direct expression
techniques, including antibody screening of fusion proteins encoded
by cDNA cloned in .lamda.GT11 (Young and Davis, 1983; Young and
Davis, 1984); or activity assay of oocyte-conditioned media after
injection of mRNA from cloned cDNA, or from plasmid or phage DNA
carrying SP6/T7 promoters. Alternatively, one can make libraries in
plasmid, phage, and cosmid vectors containing a variety of
promoter, selection and replication elements. Animal cells may be
transfected with the library for transient or stable expression.
Transfection can be accomplished by a variety of methods. For
transient expression, investigators have used spheroplast fusion,
DEAE dextran, and electroporation. For stable expression they have
used calcium phosphate, spheroplast fusion, and electroporation. We
used COS 7 cells, a transient expression system, transfected by
spheroplast fusion. (Aruffo and Seed, 1987.)
[0049] Until recently, identification of cloned molecules by direct
expression has required sensitive assays and has been restricted to
lymphokines. However, cDNA cloning of single-chain cell-surface
molecules in efficient transient expression vectors (see, e.g.,
Seed and Aruffo, 1987 and Seed, 1987), either by antibody "panning"
technology (Wysocki and Sato, 1978) or by identification of
functional molecules by FACS (Yamasaki et al., 1988), has expanded
the range of cloned molecules that one can identify by direct
expression. We have extended this technology by using an adhesion
assay in that an appropriate cell type, expressing the ligand for
the cloned molecule, is used to identify that molecule.
[0050] We detected ELAM expression by testing the ability of
transfected cells to bind either the human neutrophil-like cell
line, HL-60 (Bevilacqua et al., 1985), or the human
B-lymphocyte-like cell line, RAMOS (American Type Culture
Collection, ATCC accession no. CRL 1596, human Burkitt lymphoma).
We describe this in more detail below. Because the transfected
cells were non-human, those producing human ELAM polypeptides did
so in substantially purified form and essentially free of normally
associated animal proteins. We picked cells that tested positive in
this assay, collected the plasmid DNA, and isolated the inserts
from them. These inserts contained DNA sequences encoding ELAM1
(selected by adhesion to HL-60 cells) and VCAM1 (selected by
adhesion to RAMOS cells).
[0051] In a second method, we identified cDNA inserts from the
enriched sublibrary that hybridized on a Northern blot to a 4 kb
band of induced, but not uninduced, mRNA. Two of these inserts
contained DNA sequences for ELAM1. Other inserts from the
sublibrary encode different induced mRNAs.
[0052] We isolated a cDNA for another VCAM, VCAM1b, by probing the
IL-1.beta.-induced HUVEC cDNA library with a random-primed
oligonucleotide .sup.32P-labeled probe derived from the VCAM1 cDNA
sequence. VCAM1b is larger than VCAM1.
[0053] Using the clones identified by these three methods, we
determined the sequences of cDNAs for ELAM1 and VCAM1 and 1b. It
should be noted that due to the degeneracy of the genetic code, one
may alter many of the nucleotides of these sequences and retain DNA
sequences that code on expression for an amino acid sequence
identical to those encoded by the DNA sequences we have presented
in FIGS. 1, 3 and 4. Additionally, DNA sequences for fragments of
the ELAM cDNA sequences, or DNA sequences that are substantially
homologous to the ELAM cDNA sequences and that themselves encode
ELAM polypeptides, would hybridize to the disclosed ELAM cDNA
sequence under standard hybridization conditions.
[0054] From the DNA sequences described above, we deduced the amino
acid sequences of ELAM1, VCAM1 and VCAM1b. It should be clear that
given the current state of the protein-engineering art, an artisan
could make purposeful alterations, insertions or deletions in these
amino acid sequences and obtain a variety of molecules having
substantially the same biological or immunological activities as
those of the molecules we have disclosed herein.
[0055] We have also isolated genomic DNA sequences, including
transcriptional promoters, for the ELAM1 and VCAM1 and 1b genes. We
screened a human genomic library with .sup.32P-labeled probes
derived from the coding regions of the ELAM1 or VCAM1 DNA
sequences. This yielded clones that contained portions of the 5'
untranscribed and untranslated regions of both the ELAM1 and VCAM1
gene.
[0056] ELAM1 and VCAM1 transcriptional promoters have a number of
uses. First, they are useful to construct vectors inducible by
cytokines (such as TNF or IL-1), and bacterial lipopolysaccharide
(LPS), or any other agent found to induce expression of ELAMs in
endothelial cells. Such vectors may be useful, for example, in gene
transfer assays, wherein the inducible promoter is positioned so
that it drives transcription of a reporter gene such as
chloramphenicol acetyltransferase, beta-galactosidase, luciferase,
etc. This construct will then be introduced transiently or stably
into an appropriate mammalian cell line. Potential inhibitors or
stimulators of induction can then be assayed by measuring their
effect on induction by any or all of the inducers listed above.
[0057] We have also isolated a hybridoma producing monoclonal
antibodies recognizing ELAM1, designated BB11. We describe its
production in Example V, infra.
[0058] VCAM1 is involved in T and B cell binding to endothelial
cells. T cells activated by lectin stimulation or by a specific
antigen bind to HUVECs in vitro. This binding is mediated in part
by the ICAM/LFA1 pathway, since monoclonal antibodies that bind to
an inhibitory epitope on CD18 (the common B chain of LFA1)
partially inhibit T cell binding. We found that anti-CD18 and
anti-VCAM1 monoclonals completely inhibited binding. Coupled with
the observations that humans deficient in CD18 exhibit normal
recruitment of lymphocytes to sites of inflammation, and that
activated T cells do not recirculate through the lymphatic system
(i.e., they will not exit from the blood stream except at sites of
inflammation), this implies that VCAM1 is central to activated T
cell migration in vivo. Thus, VCAM1 serves to focus all activated T
cells into an inflammatory site. Since the presence of activated T
cells is the hallmark of numerous inflammatory and autoimmune
diseases, this in turn implies that inappropriate expression of
VCAM1 might be the fundamental pathochemical characteristic of such
diseases. Therefore, the VCAM1 pathway may provide a key
intervention point for diseases where activated T cell recruitment
is involved, e.g., arthritis, lupus, multiple sclerosis, etc.
Therefore, we disclose a therapeutic treatment to inhibit T cell
binding to the endothelium by blocking the VCAM1 binding pathway.
This may be accomplished by any of the means we describe
herein.
[0059] The DNA sequence of VCAM1 reveals that the molecule has no
structural similarity to ELAM1 but is a member of the
immunoglobulin supergene family. Three of the Ig superfamily
members are established cell-cell adhesion molecules. These are
NCAM, CEA, and ICAM1. NCAM binds to itself on the surface of other
cells (homotypic adhesion) thus promoting adhesion between cells of
the same type. The function of CEA was unknown until recently, when
it was discovered to function as an adhesion molecule, mediating
homotypic aggregation of colon tumor cells as well as cells
transfected with the cDNA for CEA. (Benchimol et al., 1989.) ICAM1
is a ligand for the leukocyte surface protein, LFA1, and mediates
both leukocyte-leukocyte and leukocyte-endothelial cell adhesion.
(Staunton et al., 1988.) ICAM1 and VCAM1 possess some functional
similarities, e.g., both are induced in endothelial cells after
treatment with cytokines, and both mediate adhesion of lymphocytes
and related cell lines. The ligand for ICAM1, LFA-1, has been
well-characterized. The ligand for VCAM1 has been identified as
VLA4 (see, infra). ICAM1 is believed to play a role not only in the
migration of lymphocytes to sites of inflammation in vivo but also
in numerous lymphocyte functions related to the immune response.
Additionally, ICAM1 has recently been shown to be the receptor for
many of the rhinoviruses. Receptors for other viruses (e.g., polio,
HIV) are also members of the Ig superfamily. (White and Littman,
1989.) Thus, VCAM1 may play a critical role in both immune
regulation and viral infection.
[0060] Both CEA and ICAM1 are expressed on tumor cells. CEA has
been used as a diagnostic marker for colon cancer for many years.
Recent diagnostic techniques include the use of
radioimmunoconjugates. in which anti-CEA antibodies are bound to
radioactive markers and introduced into the patient. Using this
method, clinicians have been able to identify tumors as small as
three millimeters. (Goldenberg, 1989.)
[0061] Investigators are also exploring radioimmunotherapy and
immunotoxin therapy. Radioimmunotherapy involves the use of
radioimmunoconjugates in which nuclides such as .sup.125I,
.sup.90Y, .sup.186Re and the like are bound to antibodies
recognizing a particular surface antigen. Immunotoxins are
antibodies conjugated with cell toxins, such as Pseudomonas
exotoxin and the like. Upon injection, these conjugated antibodies
target the toxic agents to cells expressing the antigen. In
accordance with this invention, radioactive markers, nuclides and
cellular toxins may be conjugated with VCAM1 and 1b or antibodies
recognizing them to target cells expressing VCAM1 ligands (e.g.,
VLA4) or VCAM1.
[0062] The discovery of new ELAMs or the future discovery of ELAMs
or MILAs being expressed on other cells, such a tumor cells, also
makes possible new TIL therapies. For example, where a tumor is
discovered which expresses an ELAM on its surface, the tumor can be
biopsied and infiltrated lymphocytes can be removed. A gene for a
tumorcidal agent, such as TNF in a retroviral expression vector, is
then used to transfect the tumor infiltrating lymphocytes (TILs),
which are then expanded with IL-2. When the transfected TILs are
injected back into the patient, the TILs are specifically directed
to the original tumor and migrate back into the tumor, where the
tumorcidal gene product is released for local effect. (See, Thomas
and Sikora, 1989.) Since all ELAMs bind some form of leukocyte and
thereby mediate infiltration, modified TIL therapies in which
infiltrated leukocyte cells are isolated, transfected for
expression of a particular desired gene product, amplified and
reintroduced to the patient are contemplated herein.
[0063] An alternative TIL therapy takes advantage of the fact that
certain cell types, notably some forms of cancer cells, express
ELAMs or MILAs. For example, colon carcinomas are known to express
CDX and melanomas express VLA4.
[0064] Employing the DNA sequences disclosed herein, a therapy can
be designed to enhance and improve the cytolytic activity of
leukocytes by transfecting them to express surface ELAMs or MILAs,
thereby improving their binding to target cells expressing the
corresponding ligand. Where the cytolytic activity of a leukocyte
cell type is increased as a function of stronger cell-cell
adhesion, such a method would improve the ability of leukocytes to
destroy targeted cells. For example, in the case of colon carcinoma
or melanoma, leukocytes (preferably infiltrating leukocytes, which
already have an affinity for the target cancer cell) may be
transfected with an expression vector including a gene for ELAM1
(in the case of colon carcinoma) or VCAM1 or VCAM1b (in the case of
melanoma). Introducing such leukocytes into the patient provides a
population of leukocytes capable of homing in on the carcinoma or
melanoma cells, respectively, which leukocytes have enhanced
ability to adhere to those cells to produce the desired cytolytic
effect.
[0065] We have also found that incubating HUVECs with TNF and
IFN-.gamma. together increases VCAM1 expression about one-hundred
percent over incubation with TNF alone. Activated T cells secrete
IFN-.gamma., and therefore may promote their own recruitment to
inflammatory sites through a positive feedback system: VCAM causes
T cell binding, T cells further stimulate VCAM production via
IFN-.gamma. secretion. Thus, we have devised a new treatment for
VCAM-dependent pathologies which involves inhibition of this
feedback mechanism. The treatment comprises inhibiting cytokines
such as IL-1, TNF or IFN-.gamma., for example with monoclonal
antibodies, to block cytokine-stimulated production of VCAM.
[0066] We have also isolated a MILA, CDX, that is involved in
ELAM1-mediated adhesion and, in fact, is probably the (or an) ELAM1
ligand. The isolation involved, as a first step, the production of
monoclonal antibodies against the CDX molecule. We immunized mice
with whole HL-60 cells, a PMN-related cell line, that was known to
bind to ELAM1. Alternatively, one could immunize with any cell line
that binds to ELAM1, including PMNs themselves and, as we shall
show, U937 cells. In addition, to isolate MILAs involved in
adhesion to other ELAMS, one could immunize with any cell line that
binds to the appropriate ELAM. For example, in isolating VCAM1, we
have identified two such cell lines: The B-lymphocyte-like cell
line, RAMOS, and the T-lymphocyte-like cell line, JURKAT.
[0067] After finding that immune serum from the immunized mice
inhibited binding of HL-60 cells to HUVECs in the adhesion assay we
will describe, we created hybridomas from spleen cells in a manner
well known to the art. (Goding, 1983.) Then we identified those
hybridomas that produced monoclonal antibodies against CDX by
testing their ability in the adhesion assay to inhibit binding of
HL-60 cells to induced HUVECs. We used several of these hybridomas
to produce ascites fluid containing monoclonal antibodies.
[0068] One can also generate monoclonal Fab fragments recognizing
these antigens using the technique of Huse et al. (1989). (See also
Skerra and Pluckthun, 1988.) Alternatively, one can produce single
domain antibodies as described by Ward et al. (1989).
[0069] Our monoclonal antibodies against CDX possess the following
characteristics: First, they inhibit binding of HL-60 cells or PMNs
to cells that express ELAM1. Second, these antibodies exhibit a
specific cell-binding pattern--they recognize cells that bind to
ELAM1, but they do not recognize cells that do not bind to ELAM1.
Third, they have a recognition pattern for human cell lines that is
distinct from the pattern of antibodies against other cell-surface
molecules, such as anti-LFA-1, anti-LFA-3, anti-CD44, anti-ICAM,
anti-CD4, and anti-Leu8.
[0070] We used these Moabs to isolate CDX. We radioactively labeled
HL-60 surface proteins and surface proteins from neutrophils
(isolated from human blood) with iodine using a modification of a
method described by Kurzinger (Kurzinger et al., 1981) or
metabolically with .sup.35S-methionine. We solubilized the membrane
proteins and incubated them with an anti-CDX monoclonal bound
through a .mu.-chain-specific rabbit anti-mouse IgG to Protein A
sepharose (ARX), and then we isolated the antibody-bound protein.
This protein is CDX isolated substantially free of normally
associated animal proteins. The protein appears on SDS-PAGE as a
single, diffuse band of about 150 kD. A 90 kD protein band was
sometimes observed in the bound proteins from HL-60 cells and
always in the proteins from neutrophils. We believe this 90 kD band
represents a CDX degradation product. We also sometimes observed
higher molecular weight bands (i.e., around 170 kD). These may be
non-specific bands. When the isolated 150 kD protein was treated
with N-glycanase, the molecular weight was reduced to approximately
70 kD. When the 150 kD band was treated with N-glycanase and
O-glycanase, the molecular weight was not further reduced. We
believe this represents the protein core of a very heavily
glycosylated protein.
[0071] We have isolated two DNA sequences, clone 7.2 and clone 1,
that appear to encode 1,3-fucosyl transferases that glycosylate the
CDX polypeptide and impart to it the ability to bind ELAM1.
1,3-fucosyl transferases are highly specific enzymes that function
in the Golgi apparatus and endoplasmic reticulum to attach fucosyl
moieties to appropriate acceptor carbohydrates through a 1,3
glycosidic linkage. The genetic structure of these sequences is
consistent with that of other, known glycosyl transferases.
Furthermore, CHO cells transfected with clone 7.2 express fucosyl
transferase activity.
[0072] Several 1,3-fucosyl transferases are known to the art.
(Paulson and Colley, 1989 and Kukowska-Latallo et al., 1990.) These
proteins of similar activity share little sequence homology between
themselves or other glycosyl transferases. (Paulson and Colley,
1989 and Kukowska-Latallo et al., 1990.) Therefore, we would not
expect these DNA sequences to share homology with the DNA sequences
of this invention. However, other species are likely to contain
homologous genes that share significant sequence homology with the
DNA sequences disclosed here. One can isolate these homologous
genes using the DNA sequences of this invention as probes under
standard hybridization conditions. This invention specifically
contemplates and encompasses such sequences.
[0073] When COS 7 cells were transfected with either of these two
clones, they behaved like cells expressing CDX, that is, they
became "visible" to ELAM1 in that they were able to produce a
surface glycoprotein to which ELAM1 binds and which are recognized
by the .alpha.-CDX monoclonal, SGB.sub.3B.sub.4. Using .alpha.-CDX
monoclonals, we immunoprecipitated a 130 kD glycoprotein from
transfected COS cells, which we have designated Pseudo-X.
Similarly, CHO cells transfected with clone 7.2 also became visible
to ELAM1 and .alpha.-CDX. They express a 140 kD glycoprotein which
we have designated Pseudo-X.sub.2.
[0074] Neither Pseudo-X nor Pseudo-X.sub.2 are CDX. Pseudo-X has a
molecular weight of about 130 kD and Pseudo-X.sub.2 of 140 kD. CDX
has a molecular weight of 150 kD. When treated with N-glycanase or
hydrofluoric acid (which removes all carbohydrate), Pseudo-X shifts
to 110 kD. Pseudo-X.sub.2 shifts to approximately 120 kD. CDX
shifts to about 70 kD. Neither migrates at 46 kD or 59 kD, the
predicted molecular weights of protein 7.2 and protein 1. Pseudo-X
and CDX also have different V8 and chymotrypsin digestion
patterns.
[0075] We isolated clone 7.2 and clone 1 as follows: We created a
cDNA library from mRNA of a human cell line, HL-60, that expresses
CDX. We enriched this library by using subtraction techniques, as
we describe below, with a human cell line that does not express
CDX, in this case HeLa cells. This produced a subtracted library
containing about 2100 clones. We transfected a monkey kidney cell
line, COS 7, with the subtracted library which we assayed in a
number of ways.
[0076] We incubated the transfected cells with the .alpha.-CDX
monoclonal antibodies and panned them on plates coated with
anti-mouse IgG or IgM (Wysocki and Sato, 1978); cells binding to
the plates would be those expressing a molecule recognized by
.alpha.-CDX Moabs. In this manner, we identified adherent cells
transfected with a 2.1 kb DNA insert. We subcloned a portion of
this sequence into a sequencing vector and designated it pSQ219.
The DNA insert in the pCDM8 clone was designated clone 7.2. We also
isolated a 2.9 kb insert by hybridization, which we designated
clone 1. These two clones encode protein 7.2 and protein 1,
respectively.
[0077] We are also isolating a DNA sequence that codes on
expression for CDX using techniques known to the art. Some
practical techniques involve using expression systems to express
cloned DNA. As we have mentioned, a variety of eukaryotic
expression systems are available.
[0078] One can isolate a DNA sequence encoding CDX using antibodies
that recognize the CDX polypeptide, rather than the CDX
glycoprotein. These antibodies are used to probe an HL-60 cDNA
library like the one we described above.
[0079] Another method for isolating a DNA sequence encoding CDX (or
another MILA) would employ fluorescent-antibody labeling. In this
method, CDX-expressing cells are incubated with .alpha.-CDX Moabs
and then the Moabs are labeled with, e.g., fluorescently tagged
anti-mouse antibody. Cells binding the fluorescent antibodies may
then be sorted with a fluorescence activated cell sorter (FACS).
The DNA from the sorted cells may be used to transform a bacterial
host such as E. coli. DNA from the resulting colonies may then be
used to transfect COS 7 cells, and this procedure may be repeated
until a single CDX-expressing clone is identified.
[0080] A third method is to pan the transfected cells as described
above on plates coated with recombinant soluble ELAM1 (rsELAM1). We
describe a method to coat plates with rsELAM1 in Example VIII.
Cells binding to the plates will be those expressing CDX. Other
soluble ELAMs can similarly be used to isolate cells expressing
their ligands or MILAs involved in their adhesion pathways.
[0081] An expression library may also be created in E. coli. For
example, a .lamda. ZAP.RTM. (Stratagene)/HL-60 library may be
constructed and used to express the inserted DNA in E. coli. After
plating, the plaques can be directly screened with, e.g.,
radioactively labeled .alpha.-CDX monoclonals. (Young and Davis,
1983 and Young and Davis, 1984.) The plaques to which the
monoclonals bind can be picked and the DNA insert isolated from
them.
[0082] Another method we are using to identify ELAM ligands, not
based on antibody recognition, is to transfect COS 7 cells with an
appropriate library, that may be subtracted, and then pan them
directly onto ELAM-expressing cells (such as induced HUVECS,
ELAM-expressing COS 7 cells, or ELAM-expressing CHO cells). Once
again, multiple rounds of panning are required to enrich the
library sufficiently to isolate the pertinent clones.
[0083] Another technique for isolating the DNA sequences coding for
CDX (or other MILAS) involves screening a cDNA library with
oligonucleotide probes. By purifying a sufficient quantity of CDX,
for example by affinity chromatography using immobilized antibody
to CDX or immobilized ELAM1, one may determine a partial amino acid
sequence and synthesize oligonucleotide probes that correspond to
at least a portion of the CDX gene. These probes may then be used
to screen the cDNA library. Alternatively, the oligonucleotides may
be used as primers to generate long probes to be used in screening
the library for CDX (MILA) genes.
[0084] We have also identified a ligand for VCAM1 and VCAM1b. It is
the integrin VLA4. (Hemler, 1988; Hemler et al., 1987a; and Hemler
et al., 1987b.) The integrins are a group of cell-extracellular
matrix and cell-cell adhesion receptors exhibiting an .alpha..beta.
heterodimeric structure. (Hynes, 1987; Marcantonio and Hynes,
1988.) Investigators have identified three subfamilies of integrins
categorized according to the 8 subunit. The VLA (Very Late Antigen)
proteins belong to the .beta..sub.1 subfamily, many of whose
members are specialized for cell-extracellular matrix attachment.
(Hynes, 1987 and Ruoslahti, 1988.) VLA4 is expressed in relatively
high levels on lymphoid cells (such as B and T cells) and myeloid
cells, but is hardly detectable in other cells (Hemler et al.,
supra.) The binding of B and T cells to the extracellular matrix is
mediated by VLA4 and its ligand, human fibronectin (FN). (Wayner et
al., 198'.) The discovery that VLA4 is a ligand for VCAM1 is
important because it now defines one binding pathway of B and T
lymphocytes to activated endothelial cells. Therefore, we describe
the use of VLA4 and VCAM1 and 1b as ligand and receptor in the
methods described below.
[0085] We contemplate several uses for ELAM and MILA DNA sequences
and molecules in the present invention. First, one may use ELAMs
and MILAs to produce monoclonal antibody preparations that are
reactive for these molecules. The Moabs may be used in turn as
therapeutic agents to inhibit leukocyte binding to endothelial
cells.
[0086] Second, one may use a soluble form of ELAM, soluble ELAM
ligand, or fragments of either as binding inhibitors. The ELAM
peptides would bind to the ELAM ligand on leukocytes, and the ELAM
ligand would bind to ELAM on endothelial cells. Both methods would
thereby inhibit leukocyte binding to endothelial cells. To produce
recombinant soluble ELAM (rsELAM) or rsELAM ligand one preferably
would alter a DNA encoding those molecules to eliminate the
transmembrane region. Thus, DNAs for soluble molecules would
include all or part of the extracellular domain, perhaps attached
to the cytoplasmic domain. This approach has already been validated
using soluble CD4, the surface protein on T-cells that binds to the
AIDS virus. (Fisher et al., 1988.) This approach also avoids the
problems of antibody therapy, since the polypeptides used would be
less likely to induce an immune response.
[0087] One problem investigators have encountered with soluble
recombinant molecules is a short in vivo plasma half-life. (Capon
et al., 1989.) Because such molecules are quickly cleared from the
system, large doses or frequent injections are necessary to have a
therapeutic effect. Therefore, investigators have sought methods to
increase the half-life of soluble molecules. A potential solution
is to link the soluble molecule to another molecule known to have a
longer half-life in the blood stream. Due to their long half life,
immunoglobulin molecules are promising candidates. Capon et al.
(1989) have described the linking of soluble CD4 to an
immunoglobulin molecule using recombinant DNA techniques. In this
approach, one replaces the variable region of an immunoglobulin
molecule with the soluble protein, forming a protein/immunoglobulin
fusion protein.
[0088] It is expected that the rsELAM/immunoglobulin fusion
proteins will have greater plasma half-life than rsELAM alone. Such
fusion proteins are preferably produced with recombinant
constructs, fusing a DNA sequence encoding the soluble molecule to
a DNA sequence encoding the constant domain of an immunoglobulin
molecule. The recombinant DNA may then be expressed in an
appropriate host cell, preferably an animal cell, to produce the
fusion protein.
[0089] We expect ELAM/immunoglobulin fusion proteins to have
another advantage. Because immunoglobulin molecules are normally
bivalent (i.e., they have two binding sites) an ELAM/immunoglobulin
fusion protein would have two ELAMs and so, two ELAM ligand binding
sites. Therefore, one would expect them to have greater affinity or
avidity for cells displaying ELAM ligands.
[0090] Third, one may use molecules binding to ELAMs (such as
anti-ELAM antibodies, or markers such as the ligand or fragments of
it) to detect inflammation. This involves, for example, making a
molecule detectable by fluorescence or radioactivity, administering
it to a patient and determining where in the body it accumulates.
In this way one could also identify the type of inflammation. For
example, binding to ELAM1 would indicate acute, as opposed to
chronic inflammation.
[0091] Fourth, if an ELAM binds to its ligand through a
carbohydrate moiety or some other post-translational modification,
one could use ELAM to identify the carbohydrate on the ELAM ligand
to which it bound.
[0092] Fifth, one could use ELAMs and MILAs as part of a system to
screen small molecules for adhesion inhibitors. For example, one
could create an assay system in which small molecules are tested
for the ability to inhibit the interaction between CDX and ELAM1.
Small molecule inhibitors identified in this way would provide
candidates for anti-inflammatory drugs.
[0093] Sixth, one could use these molecules to identify endogenous
proteins that inhibit leukocyte binding to ELAMs. Investigators
have tentatively identified one such molecule, leukocyte adhesion
inhibitor (LAI), that is involved in detaching bound PMNs from
endothelium. (Wheeler et al., 1988.)
[0094] Seventh, one can generate VCAM/ICAM fusion proteins. We know
that both proteins are composed of several structural domains.
(Simmons et al., 1988.) DNA sequences encoding various domains of
each protein are fused using, for example, the genetic fusion
techniques we describe for making ELAM/immunoglobulin fusion
proteins. The domains chosen are those having the ability to bind
VCAM1 or VCAM1b ligands and ICAM1 ligands, respectively. Domains
binding VLA4 and LFA1, the known ligands, are preferable. The
polypeptides produced on expression of these DNA sequences are
useful because they would block adhesion of any cell having a
ligand to either VCAM1 or VCAM1b, or ICAM1 or both.
[0095] Finally, one could use ELAM and ELAM ligand DNA sequences to
produce nucleic acid molecules that intervene in ELAM or ELAM
ligand expression at the translational level. This approach
utilizes antisense nucleic acid and ribozymes to block translation
of a specific mRNA, either by masking that mRNA with an antisense
nucleic acid or cleaving it with a ribozyme. These methods will be
useful in treating inflammatory conditions.
[0096] Antisense nucleic acids are DNA or RNA molecules that are
complementary to at least a portion of a specific mRNA molecule.
(See Weintraub, 1990; Marcus-Sekura, 1988.) In the cell, they
hybridize to that mRNA, forming a double stranded molecule. The
cell does not translate an mRNA in this double-stranded form.
Therefore, antisense nucleic acids interfere with the expression of
mRNA into protein. Oligomers of about fifteen nucleotides and
molecules that hybridize to the AUG initiation codon will be
particularly efficient, since they are easy to synthesize and are
likely to pose fewer problems than larger molecules when
introducing them into ELAM-producing cells. Antisense methods have
been used to inhibit the expression of many genes in vitro.
(Marcus-Sekura, 1988; Hambor et al., 1988.)
[0097] Ribozymes are RNA molecules possessing the ability to
specifically cleave other single stranded RNA molecules in a manner
somewhat analogous to DNA restriction endonucleases. Ribozymes were
discovered from the observation that certain mRNAs have the ability
to excise their own introns. By modifying the nucleotide sequence
of these RNAs, researchers have been able to engineer molecules
that recognize specific nucleotide sequences in an RNA molecule and
cleave it. (Cech, 1988.) Because they are sequence-specific, only
mRNAs with particular sequences are inactivated.
[0098] Investigators have identified two types of ribozymes,
Tetrahymena-type and "hammerhead"-type. (Hasselhoff and Gerlach,
1988.) Tetrahymena-type ribozymes recognize four-base sequences,
while "hammerhead"-type recognize eleven- to eighteen-base
sequences. The longer the recognition sequence, the more likely it
is to occur exclusively in the target mRNA species. Therefore,
hammerhead-type ribozymes are preferable to Tetrahymena-type
ribozymes for inactivating a specific mRNA species, and
eighteen-base recognition sequences are preferable to shorter
recognition sequences.
[0099] The DNA sequences described herein may thus be used to
prepare antisense molecules against, and ribozymes that cleave,
mRNAs for ELAM1, VCAM1 and VCAM1b, CDX and VLA4.
[0100] Antisense molecules and ribozymes may be used in methods to
treat inflammation by introducing into cells molecules that
interfere with the expression of adhesion molecules. Since ELAMs
are induced on endothelial cells during inflammatory episodes, and
since therapeutic agents can be delivered to vascular endothelium
easily by intravenous injection, endothelial cells are attractive
targets for such therapies, provided the antisense molecules or
ribozymes can be delivered effectively to the appropriate
cells.
[0101] Investigators have suggested two approaches which could be
used to deliver these molecules to target cells. The first involves
transfecting the target cell with a vector that expresses the
anti-ELAM antisense nucleic acid or the ELAM-specific ribozyme as
an mRNA molecule. (Hambor et al., supra.) While this approach is
very useful when dealing with cell lines in vitro, it may not be as
effective in vivo. A second approach that is more promising for in
vivo delivery involves loading liposomes with anti-ELAM antisense
molecules, ELAM-specific ribozymes or vectors which express them.
These liposomes could also contain anti-ELAM monoclonal antibodies
to direct the liposome to sites of inflammation. This form of
delivery would provide a negative feedback system, since appearance
of an ELAM on a cell would make the cell a target for suppression;
and successful penetration of the antisense or ribozyme component
would halt ELAM production, thereby eliminating the cell as a
target.
[0102] Another feature of this invention is the expression of the
ELAM, MILA and other DNA sequences disclosed herein. As is well
known in the art, DNA sequences may be expressed by operatively
linking them to an expression control sequence in an appropriate
expression vector and employing that expression vector to transform
an appropriate unicellular host.
[0103] Such operative linking of a DNA sequence of this invention
to an expression control sequence, of course, includes, if not
already part of the DNA sequence, the provision of an initiation
codon, ATG, in the correct reading frame upstream of the DNA
sequence.
[0104] A wide variety of host/expression vector combinations may be
employed in expressing the DNA sequences of this invention. Useful
expression vectors, for example, may consist of segments of
chromosomal, non-chromosomal and synthetic DNA sequences. Suitable
vectors include derivatives of SV40 and known bacterial plasmids,
e.g., E. coli plasmids col E1, PCR1, pBR322, pMB9 and their
derivatives, plasmids such as RP4; phage DNAs, e.g., the numerous
derivatives of phage A, e.g., NM989, and other phage DNA, e.g., M13
and Filamentous single stranded phage DNA; yeast plasmids such as
the 2.mu. plasmid or derivatives thereof; vectors useful in
eukaryotic cells, such as vectors useful in insect or mammalian
cells; vectors derived from combinations of plasmids and phage
DNAs, such as plasmids that have been modified to employ phage DNA
or other expression control sequences; and the like.
[0105] Any of a wide variety of expression control
sequences--sequences that control the expression of a DNA sequence
operatively linked to it--may be used in these vectors to express
the DNA sequences of this invention. Such useful expression control
sequences include, for example, the early and late promoters of
SV40 or adenovirus, the lac system, the trp system, the TAC or TRC
system, the major operator and promoter regions of phage .lamda.,
the control regions of fd coat protein, the promoter for
3-phosphoglycerate kinase or other glycolytic enzymes, the
promoters of acid phosphatase (e.g., PhoS), the promoters of the
yeast .alpha.-mating factors, and other sequences known to control
the expression of genes of prokaryotic or eukaryotic cells or their
viruses, and various combinations thereof.
[0106] A wide variety of unicellular host cells are also useful in
expressing the DNA sequences of this invention. These hosts may
include well known eukaryotic and prokaryotic hosts, such as
strains of E. coli, Pseudomonas, Bacillus, Streptomyces, fungi such
as yeasts, and animal cells, such as CHO, R1.1, B--W and L-M cells,
African Green Monkey kidney cells (e.g., COS 1, COS 7, BSC1, BSC40,
and BMT10), insect cells (e.g., Sf9), and human cells and plant
cells in tissue culture.
[0107] It will be understood that not all vectors, expression
control sequences and hosts will function equally well to express
the DNA sequences of this invention. Neither will all hosts
function equally well with the same expression system. However, one
skilled in the art will be able to select the proper vectors,
expression control sequences, and hosts without undue
experimentation to accomplish the desired expression without
departing from the scope of this invention. For example, in
selecting a vector, the host must be considered because the vector
must function in it. The vector's copy number, the ability to
control that copy number, and the expression of any other proteins
encoded by the vector, such as antibiotic markers, will also be
considered.
[0108] In selecting an expression control sequence, a variety of
factors will normally be considered. These include, for example,
the relative strength of the system, its controllability, and its
compatibility with the particular DNA sequence or gene to be
expressed, particularly as regards potential secondary structures.
Suitable unicellular hosts will be selected by consideration of,
e.g., their compatibility with the chosen vector, their secretion
characteristics, their ability to fold proteins correctly, and
their fermentation requirements, as well as the toxicity to the
host of the product encoded by the DNA sequences to be expressed,
and the ease of purification of the expression products.
[0109] It will also be recognized that expression of the DNA
sequences of the present invention may have different effects in
different hosts. For example, whereas clone 7.2 expressed in COS
cells leads to the appearance of an ELAM1-binding surface molecule,
expression of clone 7.2 in, e.g., prokaryotic host cells may have
no similar effect, since prokaryotes lack internal cell structures
(e.g., Golgi apparatus) that may be necessary for the biological
functionality of protein 7.2. On the other hand, for isolation and
purification of the clone 7.2 expression product intact, host cells
in which protein 7.2 does not have a function in the cellular
biochemistry (such as the catalytic role of a glycosyl transferase)
may be preferred. The practitioner will be able to select the
appropriate host cells and expression mechanisms for a particular
purpose.
[0110] Considering these and other factors, a person skilled in the
art will be able to construct a variety of vector/expression
control sequence/host combinations that will express the DNA
sequences of this invention on fermentation or in large scale
animal culture.
[0111] Several strategies are available for the isolation and
purification of protein 7.2 and protein 1 after expression in a
host system. One method involves expressing the proteins in
bacterial cells, lysing the cells, and purifying the protein by
conventional means. Alternatively, one can engineer the DNA
sequences for secretion from cells. For example, Colley et al.
(1989) describe purifying a sialyltransferase by engineering the
cleavable signal peptide of human gamma-interferon onto the DNA
sequence for the transferase. Larsen et al. (1990) fused the DNA
sequence for protein A to the amino-terminal end of a fucosyl
transferase gene and expressed it as an excreted fusion protein. In
these constructions, one can optionally remove the transmembrane
region of these proteins that exist near the amino terminus. After
secretion the proteins are purified from the medium. Similar
strategies are available for bacteria.
[0112] Increasingly scientists are recognizing the value of enzymes
as catalysts in organic synthesis. (Wong, 1989.) The 1,3-fucosyl
transferases of this invention are useful for enzymatic synthesis
of carbohydrates in vitro. Specifically, they are useful for
catalyzing the linkage of fucose to appropriate acceptors through a
1,3 glycosidic bond. We describe one set of suitable conditions for
this catalysis in Example XII, relating to an assay for fucosyl
transferase activity. One skilled in the art will recognize other
suitable conditions under which the 1,3 fucosyl transferases
described herein may be advantageously employed.
[0113] It is now clear that the carbohydrate moiety of CDX is
important in ELAM1-mediated cell adhesion. A molecule comprising
the carbohydrate moiety of CDX, Pseudo-X or Pseudo-X.sub.2, or a
fucose-containing portion of that moiety may be sufficient to
function as an ELAM1 ligand. Such molecules may be useful in
methods, including therapies, directed to inhibiting ELAM1-mediated
cell adhesion.
[0114] This invention is also directed to small molecules that
inhibit the activity of the 1,3-fucosyl transferases described
herein, including synthetic organic chemicals, natural fermentation
products, peptides, etc. These molecules may be useful in therapies
aimed at inhibiting ELAM1-mediated cell adhesion. To identify such
molecules, one produces a test mixture by contacting together an
inhibitor candidate, a fucose acceptor and a 1,3-fucosyl
transferase. The fucose acceptor is, preferably, LacNAc or
2'-fucosyllactose. The 1,3-fucosyl transferase preferably is
derived from an extract from a cell transformed with clone 7.2 or
clone 1. Then one assays the test mixture for 1,3-fucosyl
transferase activity, such as described in Example XII.
[0115] The existence of antibodies against ELAM1, VCAM1 and 1b, CDX
and VLA4 makes possible another method for isolating other ELAMs
and ELAM ligands. The method takes advantage of an antibody
characteristic known as idiotypy. Each antibody contains a unique
region that is specific for an antigen. This region is called the
idiotype. Antibodies, themselves, contain antigenic determinants;
the idiotype of an antibody is an antigenic determinant unique to
that molecule. By immunizing an organism with antibodies, one can
raise "anti-antibodies" that recognize them, including antibodies
that recognize the idiotype. Antibodies that recognize the idiotype
of another antibody are called anti-idiotypic antibodies. Some
anti-idiotypic antibodies mimic the shape of the original antigen
that the antibody recognizes and are said to bear the "internal
image" of the antigen. (Kennedy, 1986.) When the antigen is a
ligand, certain anti-idiotypes can bind to that ligand's receptor.
Investigators have identified several of these, including
anti-idiotypes that bind to receptors for insulin, angiotensin II,
adenosine I, .beta.-adrenalin, and rat brain nicotine and opiate
receptors. (Carlsson and Glad, 1989.)
[0116] Taking advantage of this phenomenon, other ELAMs and ELAM
ligands may be isolated using anti-idiotypic antibodies.
Anti-idiotypes may be used to screen for molecules binding to the
original antigen. For example, one may use this technique to
identify other ELAM ligands.
[0117] We have demonstrated that related ELAMs exist with similar
domain structures (i.e., VCAM1 and VCAM1b.) As a result of gene
shuffling, there may be several adhesion molecules on the cell
surface that share one or more domains. Anti-idiotypic antibodies,
which recognize any shared domains, are useful to isolate
immunochemically ELAMs or ELAM-ligands not identified by bioassay,
which relies on the protein's function, rather than structure.
[0118] In order that one may better understand this invention, we
set forth the following examples. These examples are for purposes
of illustration and are not to be construed as limiting the scope
of the invention in any manner.
EXAMPLE I
Preparation of a cDNA Sublibrary Enriched for ELAM Sequences
[0119] We prepared a cDNA sublibrary enriched for ELAM sequences as
follows:
[0120] We isolated human umbilical vein endothelial cells (HUVECs)
from umbilical cords, grew the cells in primary culture, and
serially passaged them as described in Gimbrone (1976). We used
HUVECs for library construction at passages 4 or 5. To induce the
cells to produce mRNA for ELAMs we incubated confluent monolayers
for 2.5 hours at 37.degree. C. with recombinant human IL-1.beta.
(10 units/ml). We isolated the mRNA from these cells and
reverse-transcribed it into cDNA using techniques well known to the
art. (Gubler and Hoffman, 1983.) Using standard procedures, we
ligated double stranded cDNA to a NotI-BstXI linker/adaptor having
the following sequence: TABLE-US-00001 5' GCG GCC GCT TTA GAG CAC A
3' 3' CGC CGG CGA AAT CTC 5'
We then size-selected the cDNA on a 4.2 ml 5-20% potassium acetate
gradient, 2 mM EDTA, 1 .mu.g/ml ethidium bromide, in a Beckman.RTM.
SW60 Rotor for 3 hours at 50,000 rpm at 22.degree. C. according to
the protocols of Brian Seed. (See also Maniatis, 1982, p. 278.) We
pooled the cDNA fragments of greater than 500 base pairs. Then we
prepared the vector, pCDM8 (a gift from Brian Seed). We digested
this plasmid with BstXI. To remove the 400 base pair stuffer
fragment we centrifuged the mixture on a potassium acetate
gradient, as above, and isolated the large fragment. We further
purified this fragment by agarose gel electrophoresis, and then
ligated the cDNA to the vector. In this way we created recombinant
DNA molecules containing DNA sequences for mRNA expressed in
induced HUVECs. We used these plasmids to transform E. coli MC1061
P3. The result was a collection of over 7.times.10.sup.6
recombinant clones comprising a cDNA library for IL-1.beta.-induced
HUVEC mRNA.
[0121] In order to prepare from this cDNA library a sublibrary
enriched for ELAM cDNA sequences, we first prepared a subtracted
probe enriched for ELAM sequences. We prepared cDNA as above from
HUVECs induced with IL-1.beta. and labeled it with .sup.32P.
(Davis, 1986.) Then we isolated mRNA from HUVECs that had not been
induced. To subtract uninduced cDNA sequences from induced
sequences we hybridized the mRNA with the cDNA and isolated cDNA
that had not hybridized to mRNA, as described by Davis (1986). We
subjected the isolated cDNA to another round of subtraction to
increase the level of enrichment. In all, we prepared three batches
of subtracted probes enriched for ELAM sequences.
[0122] We tested the level of purification of the probe by Northern
blot. (Lehrach et al., 1977.) We ran a gel with parallel lanes of
polyA+ mRNA from induced and uninduced HUVECs and blotted it on
Gene Screen.RTM. (New England Nuclear). Hybridization and
subsequent autoradiography revealed that the probe bound strongly
to a 4 kb band in the induced lane but did not bind, beyond
background, to the uninduced lane. Occasionally we noted less
intense hybridization bands to other messages in the induced
lane.
[0123] We used the subtracted probe to create a cDNA sublibrary in
E. coli MC1061 P3 enriched for IL-1.beta. induced sequences. We
began by plating-out one million clones of the IL-1.beta.-induced
HUVEC cDNA library. We plated one million colonies on Gene Screen
Plus.RTM. filters (New England Nuclear) on LB agar containing 12.5
.mu.g/ml ampicillin and 7.5 .mu.g/ml tetracycline, and grew them at
37.degree. C. for 12 hours. We made two replicate filters (lifts)
from each master. We grew these on LB agar containing 12.5 .mu.g/ml
ampicillin and 7.5 .mu.g/ml tetracycline for 4 hours and amplified
them on LB agar containing 250 .mu.g/ml chloramphenicol for 16
hours. We lysed the filters according to manufacturer's protocol
and then prehybridized them in Plaque Screen.RTM. Buffer (0.05M
TRIS-HCl pH7.5, 1N NaCl, 1% SDS, 0.1% sodium pyrophosphate, 0.2%
polyvinylpyrrolidone (PVP), 0.2% Ficoll-400, 0.2% BSA). We
hybridized the filters at 65.degree. C. for 40 hours in 50 ml
Plaque Screens Buffer containing 10% dextran sulfate and 100
.mu.g/ml yeast tRNA and approximately 1.times.10.sup.7 cpm of the
subtracted IL-1.beta.-induced HUVEC cDNA. We then washed the
filters twice with Plaque Screen.RTM. Buffer, twice with
2.times.SSC, 1% SDS, and twice with 1.times.SSC, 1% SDS at
65.degree. C. We then exposed the filters to film for 5 days.
[0124] We selected colonies that hybridized to the probe by
aligning the master filters with the autoradiographs and scraping
the colonies off the filters with sterile toothpicks. We placed
each scraping in one well of a 96-well microtiter plate filled with
LB broth containing 7.5 .mu.g/ml tetracycline and 12.5 .mu.g/ml
ampicillin. After inoculation, we incubated the microtiter plates
overnight at 37.degree. C. When the cells had grown we added
glycerol to each well to a final concentration of 20% and stored
the plates at -70.degree. C. In this way we isolated from the
master library filters 864 colonies comprising the cDNA sublibrary
enriched for ELAM sequences. We point out that because of the
plating density, not all the colonies of the enriched sublibrary
were pure.
[0125] We carried out two sets of procedures in parallel with the
enriched cDNA sublibrary.
EXAMPLE II
Isolation of a Clone Expressing ELAM1
[0126] In a first procedure we isolated from the enriched
sublibrary a clone expressing ELAM1. We chose to transfect this
sublibrary into a cell line competent for high-level transient
expression, the African Green Monkey kidney cell line, COS 7. We
plated the cells and transfected the sublibrary by spheroplast
fusion. (Sandri-Goldin et al., 1981.) Forty-eight hours after
transfection, we assayed the COS 7 cells for expression of ELAM1 by
their ability to bind HL-60 cells, a cell line known to bind to
endothelial cells stimulated with inflammatory agents.
[0127] We performed the assay as follows: We labeled HL-60 cells
with carboxyfluorescein diacetate according to the Brenan and
Parish method. (Brenan and Parish, 1984.) Briefly, we resuspended
HL-60 cells in RPMI/10% FCS at a concentration of 1.times.10.sup.7
cells/ml, and added carboxyfluorescein diacetate to a final
concentration of 0.1 mg/ml from a stock solution of 10 mg/ml in
acetone. We incubated COS 7 cells with labeled HL-60 cells for 15
minutes at room temperature. We washed the cells 3-4 times with
RPMI/1% FCS. We examined the petri dish by fluorescence microscopy
for clusters of adherent HL-60 cells. We picked regions of the cell
plates with clusters of HL-60 cells and lysed the cells in 0.6%
SDS, 10 mM EDTA, pH 8, then rescued the plasmids according to the
method of Hirt. (Hirt, 1967.) We used these pooled plasmids to
transform E. coli MC1061 P3. We grew colonies from these
transformants and performed a second round of spheroplast fusion
with COS 7 cells with subsequent assay for HL-60 adhesion. From
among the cells that were positive for adhesion we selected one and
isolated the plasmid from it. We designated a culture containing
this plasmid ELAM pCDM8 clone 6. We deposited this plasmid under
the Budapest Treaty with In Vitro International, Inc., 611 P.
Hammonds Ferry Rd., Linthicum, Md., 21090 (USA) on Apr. 20, 1989.
It is identified as: [0128] ELAM pCDM8 clone 6/E. coli MC1061 P3
[0129] Accession Number IVI-10204
EXAMPLE III
Isolation of cDNA Inserts for ELAM1 Sequences
[0130] In a second procedure, we isolated cDNA inserts for
IL-1.beta.-induced cDNA sequences. We selected at random
twenty-four of the 864 colonies of the enriched library and
isolated plasmids from them using the alkaline miniprep procedure
of Maniatis.
[0131] (Maniatis, 1982.) We digested the plasmid DNA with XhoI or
NotI and separated the fragments on 1% agarose gels. We identified
from this gel two plasmids with inserts of greater than 3 kb,
isolated these inserts and labeled them with 2P. (See, Feinberg and
Vogelstein, 1983 and 1984.)
[0132] We then performed Northern blots with these inserts, as
described above. Both inserts hybridized to bands at 4 kb in the
induced HUVEC mRNA lane but did not hybridize to the uninduced
HUVEC mRNA lane. The inserts cross-hybridized with the ELAM1
expressing plasmid ELAM pCDM8 clone 6 (described above) as well. We
subcloned these inserts into NotI-digested pNN11 that had been
treated with calf intestinal alkaline phosphatase. We constructed
the sequencing plasmid pNN11 by removing the synthetic polylinker
from the commercially available plasmid PUC8 (Pharmacia PL
Biochemicals) by restriction digestion and replacing it with a new
synthetic segment. The 2.5 kb backbone common to the pUC plasmids,
that provides an origin of replication and confers ampicillin
resistance, remained unchanged. The novel synthetic portion of
pNN11 is shown in FIG. 2. We called these new constructs pSQ148 and
pSQ149, respectively.
EXAMPLE IV
A DNA Sequence for ELAM1
[0133] We determined the entire DNA sequence for the inserts of
plasmids pSQ148 and pSQ149 and 624 nucleotides of the sequence at
the 5' end of the insert of ELAM pCDM8 clone 6. We used the
Maxam-Gilbert method. (Maxam and Gilbert, 1980.) Because the
sequences have significant overlap, we obtained a composite
sequence of ELAM cDNA, a sequence of 3863 nucleotides. This
sequence consists of 140 nucleotides of the 5' untranslated region,
1830 nucleotides encoding 610 amino acids, and 1893 nucleotides of
the 3' untranslated region (including a translational stop codon
and a polyadenylation signal). The mature protein derived from the
deduced amino acid sequence has been designated ELAM1, and the
coding sequence has been designated the ELAM1 DNA sequence. The
cDNA sequence of ELAM1 is shown in FIG. 1.
[0134] A search of the Genbank Data Base, release 58, December
1988, revealed that the DNA sequence for ELAM1 has no significant
homologies to known DNA sequences.
[0135] We used this cDNA sequence to deduce the ELAM1 amino acid
sequence, that is also presented in FIG. 1. Our analysis of the
sequence revealed the following properties: The protein possesses a
hydrophobic N-terminal sequence characteristic of a signal
sequence. (von Heijne, 1986.) We have not yet determined the signal
cleavage site and the mature N-terminus through protein sequencing,
however based on von Heijne we predict that the mature N-terminal
amino acid will be tryptophan, at nucleotide number 204 in FIG. 1.
The extracellular domain of the polypeptide is approximately 554
amino acids including the signal sequence and is followed by a
hydrophobic transmembrane region of 24 amino acids. The protein
possesses a short, charged cytoplasmic tail of 32 amino acids. We
note that the protein is cysteine-rich and contains eleven
potential N-glycosylation sites.
[0136] When we compared the amino acid sequence of ELAM1 to other
proteins in the NBRF and NEW protein data bases we found
significant homology with several proteins, including complement C2
precursor, .beta.-2-glycoprotein I, C4b-binding protein, complement
factor B, complement factor H, Drosophila notch protein, the IgE
receptor Hepatic lectin, and Coagulation factors IX and X
precursors. Thus, we can divide ELAM1 into at least three domains
based on homology to the above-mentioned proteins: (1) a
lectin-like domain (nucleotides 204-563 of FIG. 1); (2) an EGF-like
domain (nucleotides 564-668); and (3) a consensus cysteine repeat
unit of 59-63 amino acids containing six cysteine residues per
repeat (nucleotides 669-1793). Other invariable amino acids in each
repeat are proline, glycine, and tryptophan.
EXAMPLE V
Monoclonal Antibodies Recognizing ELAM1
[0137] To make monoclonal antibodies that recognize ELAM1 we
prepared hybridomas in essentially the same manner as we did in
Example X, infra. However, we immunized the mice with
ELAM1-expressing COS cells and identified mice producing anti-ELAM1
antibodies by testing their antiserum for the ability to block
HL-60 cell adhesion to IL-1.beta. induced HUVECs.
[0138] We screened hybridomas produced in this manner for those
producing anti-ELAM1 monoclonals using several assays. First, we
tested the culture supernatants for antibodies having the ability
to bind to a cell line that stably expressed ELAM1. This cell line
was a line of CHO-DHFR.sup.- cells transfected with the ELAM1
animal cell expression vector, pBG341jod.ELAM. We created this
plasmid by introducing the DNA sequence encoding ELAM1 from pCDM8
clone 6 into the NotI site of pBG341.jod (described in Example
VIII, infra). The ELAM1 expressing CHO-DHFR.sup.- derived cell line
was detected using an adhesion assay to HL-60 cells.
[0139] Second, we screened hybridoma culture supernatants for the
ability to bind cytokine-induced, but not control, HUVECs.
[0140] Third, we tested them for their ability to inhibit HL-60
cell adhesion to cytokine-induced HUVEC monolayers.
[0141] We identified one hybridoma clone, BB11, which produced a
positive result in all three assays. BB11 immunoprecipitates
proteins with molecular weights of about 110 kD and 96 kD from
ELAM1-expressing HUVECs and COS cells, representing variably
glycosylated forms of ELAM1. (Bevilacqua et al., 1989.) It also
completely blocked adhesion of HL-60 cells to ELAM1-expressing COS
and CHO cells. It produced immunoglobulins of the IgG.sub.2b class.
We deposited a subclone of this hybridoma under the Budapest Treaty
with In Vitro International, Inc., 611 P. Hammonds Ferry Rd.,
Linthicum, Md. 21090 (USA) on Dec. 13, 1989. It is identified as:
[0142] Monoclonal antibody CDB.BB11.BC6 [0143] Accession Number
IVI-10220.
EXAMPLE VI
Isolation of Clones Expressing VCAM1 and VCAM1b
[0144] We have also characterized and cloned two different ELAMs
that bind to lymphocytes and lymphocyte-like cell lines. As a first
step, we characterized the binding pathways of RAMOS, a
B-lymphocyte-like line, and JURKAT, a T-lymphocyte-like line, to
HUVECs induced with IL-1.beta. or TNF for 4, 24, or 48 hours. We
found that both RAMOS and JURKAT binding was maximal at 4 hours
after induction with either IL-1.beta. or TNF, and binding was
maintained at 24 hours and 48 hours after induction. RAMOS binding
was temperature-sensitive, occurring at room temperature but not at
4.degree. C. JURKAT binding was reduced but not completely
eliminated at 4.degree. C., and thus JURKAT exhibited both a
temperature-sensitive and temperature-insensitive component.
Antisera from mice immunized with JURKAT cells inhibited binding
from both JURKAT and RAMOS cells to HUVECs, indicating that RAMOS
and JURKAT share a MILA. Neither RAMOS nor JURKAT bound to COS or
CHO cells expressing ELAM1, indicating the presence of at least one
other inducible ELAM on HUVECs, at 4 to 48 hours after
induction.
[0145] In order to isolate clones expressing the ELAMs involved in
RAMOS and JURKAT binding to HUVECs, we screened the previously
described ELAM-enriched HUVEC cDNA sublibrary by the method
described in Example II, supra. We incubated carboxy-fluorescein
diacetate-labeled RAMOS and JURKAT cells with
sublibrary-transfected COS 7 cells. Regions of the cell plates with
clusters of bound cells were picked and lysed, and the plasmids
were rescued, transformed into E. coli, and reassayed in COS 7
cells as previously described. Plasmids were isolated from
individual bacteria colonies from the transformants that were
positive on reassay. These plasmids were transfected individually
into COS 7 cells, and a plasmid that tested positive for adhesion
to RAMOS and JURKAT was identified. The cDNA insert from this
plasmid was excised, radioactively labeled, and used to probe a
Northern blot according to the procedures of Lehrach (1979). The
probe hybridized to an RNA species approximately 3.4 kb in length.
The RNA was undetectable in uninduced HUVEC RNA, barely detectable
at 5, 10, 30 or 60 minutes after treatment with IL-1.beta., but
abundant at 2, 24, 48 and 72 hours after treatment with
IL-1.beta..
[0146] We designated the plasmid AM pCDM8 clone 41. We deposited
this plasmid under the Budapest Treaty with In Vitro International,
Inc., Linthicum, Md. (USA) on May 24, 1989. It is identified as:
[0147] AM pCDM 8 clone 41/E. coli MC1061 P3 [0148] Accession Number
IVI-10206
[0149] We have also isolated a cDNA for another VCAM. We screened
the IL-1.beta.-induced HUVEC cDNA library (Example I) with a
labeled VCAM1-encoding insert from AM pCDM 8 clone 41. We sequenced
one of these, clone 1E11. We found several clones that were longer
than the clone 41 insert as analyzed by restriction mapping with
XbaI. We sequenced one of these, clone 1E11. We deposited it under
the Budapest Treaty with In Vitro International, Inc., Linthicum,
Md. (USA) on Dec. 7, 1989. It is identified as: [0150] VCAM 1B
Clone 1E11 pCDM8/E. coli MC1061p3 [0151] Accession Number
IVI-10216.
[0152] We are also isolating DNA sequences for other ELAMs. We are
collecting mRNA from HUVECs around forty-eight hours after
IL-1.beta. induction. We will isolate the ELAM cDNA sequences in a
manner similar to the one we used to isolate the cDNA sequences for
ELAM1 and VCAM1 and 1b.
[0153] Alternatively, one may identify other ELAMs by inducing
cells with other inflammatory agents, such as TNF, LT, LPS,
interferons, or combinations of such agents.
EXAMPLE VII
DNA Sequences for VCAM1 and VCAM1b
[0154] We determined the entire DNA sequence for the insert of
plasmid AM pCDM8 clone 41 by the method of Maxam and Gilbert
(1980). This sequence consists of 106 nucleotides of the 5'
untranslated region, 1941 nucleotides encoding 647 amino acids, and
764 nucleotides of the 3' untranslated region including a
translational stop codon. The protein derived from the cDNA
sequence has been designated VCAM1, and the coding sequence has
been designated the VCAM1 DNA sequence. We have presented the cDNA
sequence of VCAM1 in FIG. 3. The putative amino acid sequence of
VCAM1 is also indicated in FIG. 3.
[0155] We also determined the entire DNA sequence for the insert of
plasmid VCAM1b pCDM8 1E11 by the method of Maxam and Gilbert
(1980). This sequence consists of 99 nucleotides of the 5'
untranslated region, 2217 nucleotides encoding 739 amino acids and
764 nucleotides of the 3' untranslated region including a
translational stop codon. We have designated the mature protein
derived from the cDNA sequence as VCAM1b and the coding sequence as
the VCAM1b DNA sequence. We have presented the cDNA sequence and
putative amino acid sequence of VCAM1b in FIG. 4.
[0156] Comparison of the DNA and amino acid sequences of VCAM1 and
VCAM1b revealed that they are virtually identical except for one
significant difference: VCAM1b contains an insertion of 276
nucleotides near the middle of the coding region. These nucleotides
encode 92 additional amino acids which form an extra domain of 84
amino acids situated between the end of VCAM1 domain 3 and the
beginning of VCAM1 domain 4. We discuss the significance of this
domain, designated VCAM1 domain 3B, below.
[0157] Our analysis of the sequences revealed the following
properties: The VCAM1 polypeptide possesses a hydrophobic
N-terminal sequence characteristic of a signal sequence. (von
Heijne, 1986.) We have not yet determined the signal cleavage site
and the mature N-terminus through protein sequencing, however based
on von Heijne we predict that the N-terminal amino acid of the
mature protein will be phenylalanine, at nucleotide number 179 in
FIG. 3. The extracellular domain of the polypeptide is
approximately 606 amino acids including the signal sequence and is
followed by a hydrophobic transmembrane region of 22 amino acids.
The protein possesses a short, charged cytoplasmic tail of 19 amino
acids. We note that the protein contains six potential
N-glycosylation sites.
[0158] Similarly, the N-terminal amino acid of the mature VCAM1b
protein should be the phenylalanine, at nucleotide number 172 of
FIG. 4. The extracellular domain of the polypeptide, which is
longer than VCAM1, is approximately 698 amino acids including the
signal sequence and is followed by a hydrophobic transmembrane
region of 22 amino acids. The protein possesses a short, charged
cytoplasmic tail of 19 amino acids. We note that the protein
contains seven potential N-glycosylation sites.
[0159] Comparison of the amino acid sequences of VCAM1 and VCAM1b
with other proteins in the NBRF and NEW protein databases revealed
significant homologies with several proteins, including
non-specific cross-reactive antigen (NCA), biliary glycoprotein 1
(BG1), neural cell adhesion molecule (NCAM), carcinoembryonic
antigen (CEA), immunoglobulin alpha chain constant region, the T
cell receptor (TCR) alpha and beta chain variable regions, and
myelin associated glycoprotein (MAG). Lesser homology is seen with
myosin light chain kinase, ribulose biphosphate carboxylase,
adenovirus E1A 28K protein, pseudouridine synthetase, and
xylulokinase. VCAM1 and 1b and the VCAM1 and 1b DNA sequences show
no homology with, and are distinct from, the previously described
ELAM1 (supra).
[0160] Importantly, NCA, BG1, NCAM, CEA, MAG, and TCR are members
of the immunoglobulin gene superfamily. (Williams and Barclay,
1988; Hunkapiller and Hood, 1989.) Members of this family are
defined by the presence of one or more regions homologous to the
basic structural unit of immunoglobulin (Ig) molecules, the Ig
homology unit. (Hunkapiller and Hood, 1989.) These units are
characterized by a primary amino acid sequence of about 70-110
residues in length, with an essentially invariant disulfide bridge
spanning 50-70 residues, and several other relatively conserved
residues involved in establishing a tertiary structure referred to
as the "antibody fold". These units may be further subdivided into
three groups, i.e., V, C1, and C2 (Williams and Barclay, 1988), or
V, C, and H (Hunkapiller and Hood, 1989), based on various
criteria, including intercysteine spacing, number of beta strands,
and type of conserved residues. When these criteria are applied to
the predicted primary sequence of VCAM1, the sequence can be
divided into six Ig units, designated domains 1-6, all of which
fall into the C2 or H subset, each of about 100 amino acids in
length. The invariant disulfide bridges of the six domains,
referring to FIG. 3, occur between cysteines 47 and 95 (domain 1),
137 and 195 (domain 2), 246 and 291 (domain 3), 333 and 391 (domain
4), 442 and 487 (domain 5), and 531 and 576 (domain 6).
[0161] As we stated above, VCAM1b has seven domains. We have
designated the additional domain as domain 3B. This domain is
included in the additional 276 nucleotides of VCAM1b that begin at
nucleotide 1027 and end at nucleotide 1305 of FIG. 4. The DNA
sequence encompassing domains 1-3 is 72% homologous to the DNA
sequence encompassing domains 3B-5. At the polypeptide level, there
is significant homology between domains 1 and 3B, 2 and 4, and 3
and 5, respectively. We present the domain structures of VCAM1 and
VCAM1b in FIGS. 5 and 6.
[0162] Messenger RNAs for VCAM1 and VCAM1b could arise by two
mechanisms: They could represent alternately spliced forms of the
same gene product, or they could be the products of separate VCAM
alleles. To help distinguish between these possibilities, we
examined VCAM1 and mRNA from three individuals, at different
time-points after cytokine induction. HUVECs were prepared from
umbilical cords from three different individuals, the cord samples
being labeled #1, #2 and #3. Each preparation was split into four
separate flasks for treatment with TNF for 0 (untreated), 2.5, 24,
and 48 hours. Relative amounts of VCAM1 and VCAM1b mRNA were
determined by Northern blotting and probing with synthetic
oligonucleotides specific for each form. VCAM1b was clearly the
major mRNA present in all three umbilical cord preparations. VCAM1
was present in cords #1 and #3, most prominently at the 2.5 hour
induction time-point, although in cord #3 VCAM1 was also present at
24 and 48 hours. Cord #12 cells had little or no VCAM1 mRNA,
although amounts of VCAM1b mRNA were comparable to those in HUVECs
from cords #1 and #3. The mechanism by which these two products
arise is still unclear, although alternate splicing seems likely
because the two mRNAs are identical except for the deletion of one
domain, at a point likely to be a splice junction, judging by its
position between domains (Hunkapiller and Hood, 1989) and by the
presence of the dinucleotide AG, typical of splice junctions
(Breathnach and Chambon, 1981). Furthermore, alternate splicing is
common among other members of the Ig gene superfamily to which
VCAM1 is most clearly related. (Hunkapiller and Hood, 1989.)
[0163] Functionally, differences between the two forms of VCAM1
appear to be minimal. Both forms, when expressed transiently in COS
7 cells, bound RAMOS cells, and this binding was completely
inhibited by Moab 14B9, indicating that the same epitope is
relevant to binding in each case. Furthermore, we have shown that
this epitope is located within the first three domains, which are
common to both forms (see Example VIII, supra).
EXAMPLE VIII
Recombinant Soluble ELAM1 and VCAM1b
[0164] We constructed a vector expressing recombinant soluble ELAM1
(rsELAM1). We called this vector pSAB108. The rsELAM1 expressed by
pSAB108 contains the portion of the extracellular domain of ELAM1
encoded by the DNA sequence of FIG. 1 from nucleotide 141 to
nucleotide 1790.
[0165] To construct pSAB108 we first created a DNA fragment which
encoded an rsELAM1. We digested ELAM pCDM8 clone 6 with MluI and
NotI. This yielded a 3.8 kb DNA fragment including a DNA sequence
encoding ELAM1. We subcloned this fragment into NotI-digested pNN11
that had been treated with calf intestinal alkaline phosphatase
(described in Example III). We called this vector pNNELAM1.
[0166] We used site specific mutagenesis to eliminate the
transmembrane and intracellular regions of ELAM1. (Peden and
Nathans, 1982; Kalderon et al., 1982; Oostra et al., 1983.)
Accordingly, we digested a sample of pNNELAM1 with EcoRI and
isolated the large fragment. We linearized another sample of
pNNELAM1 with ScaI. Then we synthesized an oligonucleotide having
the sequence 5' TGT GAA GCT CCC TAA ATT CCC. When this sequence
hybridizes to an ELAM1 antisense sequence it introduces a stop
codon and a BamHI restriction site into the ELAM1 DNA sequence
after nucleotide number 1790. We created a heteroduplex using these
three fragments according to the methods of Morinaga et al. (1984)
and Chang et al. (1984). We filled in the single stranded gaps with
Klenow fragment and T4 ligase and used the mixture to transform E.
coli MC1061. We screened the resulting colonies by checking for a
BamHI site and selected mutagenized clones. Consequently on
expression, the transmembrane region of the polypeptide is
eliminated and the C-terminal amino acid is proline. We called this
plasmid pSAB100.
[0167] Then we digested pSAB100 with AatII and NcoI and isolated
the 5.2 kb fragment. We also digested pNNELAM1 with these two
enzymes and isolated the 1.4 kb fragment. NcoI cuts at nucleotide
927 of FIG. 1, about the middle of the ELAM1 coding area. We
ligated these two DNA fragments and called the plasmid pSAB108. We
made this construction because site-directed mutagenesis sometimes
causes mutations in other parts of the molecule and we wanted to
avoid any such mutations in the coding region or rsELAM1. We
digested pSAB108 with NotI and isolated the 3.8 kb fragment. We
ligated this fragment to a 7819 bp fragment of pBG341.jod, created
as follows.
[0168] First we obtained pSV2-DHFR, ATCC 37146, from the American
Type Culture Collection, Bethesda, Md. (USA). (Subramani et al.,
1981.) We digested this with ApaI and EcoRI and isolated the 4420
bp fragment. Then, we produced a synthetic double stranded DNA
sequence having an ApaI overhang, a DNA sequence encoding
nucleotides +190 to +233 of the human gastrin gene (Sato et al.,
1986, FIG. 4), an XhoI site, and an EcoRI overhang. We ligated this
oligonucleotide with the 4420 bp fragment of pSV2-DHFR and called
the resulting plasmid pDT4. We digested this plasmid with AatII and
XhoI and isolated the 4391 bp fragment.
[0169] Then we cleaved the Mullerian Inhibiting Substance
expression vector pD1 (Cate et al., 1986) with AatII and SalI and
isolated the 5462 bp fragment. We ligated this fragment with the
4391 bp fragment of pDT4 to make pJOD-10.
[0170] We digested pJOD-10 with HindIII and BstEII and isolated the
large fragment which did not encode Mullerian Inhibiting Substance.
We blunt-ended the fragment ends, ligated SalI linkers to the ends
and self-ligated the vector. This produced pJOD-s.
[0171] Then we digested pJOD-s with AatI and NotI and isolated the
6750 bp fragment. We ligated this to a 1100 bp NotI fragment from
pBG341, which we created as follows.
[0172] We created pBG341 by replacing the SmaI site of pBG312 (Cate
et al., 1986) with a NotI site. We linearized pBG312 with BqlII,
blunt-ended the fragment by filling in with Klenow, and
self-ligated it. We linearized this plasmid with BamHI and again
blunt-ended and self-ligated it. We linearized this plasmid with
SmaI and ligated to the ends a NotI linker having the sequence 5'
GCGGCGC. We called the resulting plasmid pBG341.
[0173] We digested pBG341 with AatII and NotI and isolated the 1100
bp fragment. We ligated this fragment to a 6750 bp fragment of
pJOD-s. We called the resulting plasmid pBG341.jod. This plasmid
contains the SV40 early and the adenovirus major late promoter.
Genes inserted into the plasmid at the NotI site are transcribed
from either of these promoters.
[0174] Then we linearized pBG341.jod with NotI and isolated the
linear 7819 bp fragment. We ligated this fragment with the 3.8 kb
fragment of PSAB108, which encoded rsELAM1, generating plasmid
pSAB110.
[0175] We transfected CHO-DHFR cells by electroporation with
plasmid pSAB110 linearized with AatII. We performed electroporation
with a Biorad.RTM. Gene Pulser at 270V and 960 .mu.FD using
10.sup.7 cells/ml in 20 mM HEPES pH 7.05, 137 mM NaCl, 5 mM KCl,
0.7 mM Na.sub.2HPO.sub.41 and 6 mM dextrose with 20 .mu.g plasmid
and 200 .mu.g sonicated salmon sperm DNA. Following transfection we
cultured the cells in selective medium, alpha.sup.- MEM containing
500nM, methotrexate and 10% dialyzed FCS. We picked colonies,
plated them onto 96-well cluster plates and detected
rsELAM1-expressing cells using the monoclonal antibody BB11. After
growing cells to confluence in complete medium containing 10% fetal
calf serum (FCS) we maintained them in medium containing 2% FCS in
which the cells produced rsELAM1. We harvested medium and replaced
it with fresh 2% serum every three or four days.
[0176] We isolated rsELAM1 from this conditioned medium to at least
95% purity. This involved concentrating the medium and incubating
it overnight with Moab BB11 (Example V) covalently coupled to
Protein A sepharose. (Schneider et al., 1982.) Then we washed this
resin with PBS to remove unbound protein, eluted bound material
with 0.1 M glycine, pH 2.7, neutralized the eluate with sodium
phosphate and dialyzed it against PBS. We further purified the
rsELAM1 by chromatography with Protein A sepharose in PBS.
[0177] Using the following assay, we demonstrated that we had
produced rsELAM1. To a 6 cm diameter Petri dish of bacteriologic
plastic (e.g., Falcon #1007.RTM.) we added 2.5 ml of 50 mM Tris
buffer, pH 9.5. To this we added 10 .mu.g of pure rsELAM1. We
incubated the plate for 60 minutes at room temperature to allow the
rsELAM1 to bind to the plate. Then we aspirated the medium and
replaced it with PBS containing 10 mg/ml bovine serum albumin. We
incubated the plates overnight at 4.degree. in this solution to
block remaining protein binding sites on the plates. We warmed the
plates to room temperature, washed them with medium containing 10%
fetal calf serum, and incubated them with 2 ml of cells
(2.times.10.sup.6 ml.sup.-1) for 20 minutes. We aspirated the
medium and washed the plates twice with 3 ml each of medium (RPMI
1640 with 10% serum). Then we examined the plates by
microscopy.
[0178] We found that cells which bind ELAM1, such as HL-60 cells,
bind to rsELAM1-coated plates, while cells which do not bind to
ELAM1, e.g., the B cell line RAMOS, do not bind to these
plates.
[0179] In addition, we found that the specific Moab BB11 blocks the
binding of HL-60 cells to rsELAM1 coated plates. Together, these
results show first, that we have produced rsELAM1, and, second,
that like ELAM1, rsELAM1 possesses the ability to bind to
leukocytes.
[0180] We also constructed a vector expressing recombinant soluble
VCAM1b (rsVCAM1b). This vector was named pBN1006, and the rsVCAM1b
expressed by pBN1006 contains the portion of the extracellular
domain of VCAM1b encoded by the DNA sequence shown in FIG. 4 from
nucleotide 107 to nucleotide 2193.
[0181] In order to generate a cell line capable of constitutively
expressing full length soluble VCAM1b, we first created a vector
derived from pJOD-s having a unique NotI site downstream from the
adenovirus major late promoter, so that NotI fragments could be
inserted into the expression vector. pJOD-s was linearized by NotI
cleavage of the plasmid DNA. The protruding 5' termini were
blunt-ended using Mung-bean nuclease and the linearized DNA
fragment was purified by low melting temperature agarose (LMA) gel
electrophoresis. The DNA fragment was religated using T4 DNA
ligase. The ligated molecules were then transformed into E. coli
JA221 (ATCC accession no. 33875). Colonies were screened for the
absence of a NotI site. The resulting vector was designated as
pJOD-s delta Not1. pJOD-s delta Not1 was linearized using SalI and
the 5' termini were dephosphorylated using calf intestine alkaline
phosphatase. The linearized DNA fragment was purified by LMA gel
electrophoresis and ligated in the presence of phosphorylated
oligonucleotide ACE 175 (5' pTCGACGCGGCCGCG). The ligation mixture
was transformed into E. coli JA221 and colonies were screened for
the presence of a NotI site. The correct plasmid was named
pMDR901.
[0182] Soluble VCAM1b was obtained by truncating VCAM1b clone 1E11
at nucleotide 2193 by digestion with AluI, thus eliminating the
transmembrane and intracellular portion as well as the 3'
untranslated region. A stop codon-NotI linker was added, and the
insert was religated into pCDM8. The insert was excised from PCDM8
with NotI and ligated into pMDR901 at the NotI site. This
construct, designated pBN1006, encodes full length soluble VCAM1b,
having amino acids 1-698 as shown in FIG. 4.
[0183] Using materials and methods already described, we have also
constructed plasmids expressing truncated forms of the rsELAM1 and
rsVCAM1b molecules described above. These truncated forms,
comprising the amino acid sequences of one or more of the
particular domains of the extracellular regions of ELAM1 and
VCAM1b, were used to investigate which domain or domains are
involved most directly in cell-to-cell adhesion. Our initial
experiments have investigated the domains of ELAM1 and VCAM1 and 1b
that are recognized by antibodies against those molecules, i.e.,
antibodies BB11 and 4B9, respectively.
[0184] A soluble ELAM1 construct designated CH101 was prepared
comprising the lectin-like domain of ELAM1. Referring to FIG. 1,
CH101 was the expression product of a cDNA sequence including
nucleotides 1-557 (coding for amino acids 1 through 139 of ELAM1)
and a stop codon. Another soluble construct designated CH102 was
prepared comprising the lectin-like domain and the EGF-like domain
of ELAM1. Referring to FIG. 1, CH102 was the expression product of
a cDNA sequence that included nucleotides 1-671 (coding for amino
acids 1 through 177 of ELAM1) and a stop codon. The soluble ELAM1
construct CH102 was found to immunoprecipitate the anti-ELAM1
monoclonal antibody, BB11.
[0185] The following soluble VCAM1 and 1b constructs were similarly
prepared:
[0186] (A) domain 1 (nucleotides 1-430 of FIG. 3, coding for amino
acids 1-108);
[0187] (B) domain 1+domain 2 (nucleotides 1-757 of FIG. 3, coding
for amino acids 1-217);
[0188] (C) domain 1+domain 2+domain 3 (nucleotides 1-1036 of FIG.
3, coding for amino acids 1-310);
[0189] (D) domain 1+domain 2+domain 3 (from a hybrid of VCAM1 and
VCAM1b cDNA, coding for amino acids 1-317 as depicted in FIG.
4);
[0190] (E) full length soluble VCAM1 (nucleotides 1-1924 of FIG. 3,
coding form amino acids 1-606); and
(F) full length soluble VCAM1b (nucleotides 1-2193 of FIG. 4,
coding for amino acids 1-698).
[0191] Of the foregoing VCAM1 constructs, B, C, D, E and F (but not
A) were immunoprecipitated with the anti-VCAM1 antibody 4B9.
Constructs B. D, E and F were also found to produce protein
functional for cell adhesion. Conditioned media containing protein
encoded by constructs B, D, E and F were concentrated, passed over
an immunoaffinity column of immobilized 4B9 antibody, and the bound
protein eluted and neutralized as described for rsELAM1. The eluted
proteins were immobilized on plastic as described for rsELAM1 and
found to support specific adhesion of RAMOS and JURKAT cells. These
results indicate that the first two domains of VCAM1 are sufficient
to support adhesion of certain VLA4-expressing human lymphoid cell
lines.
EXAMPLE IX
Isolation of the ELAM1 and VCAM1 Promoter
[0192] We have isolated and characterized genomic clones for the
ELAM1 and VCAM1 genes. We isolated the ELAM1 clones as follows:
[0193] We selected as probes either the entire ELAM pCDM8 clone 6
insert or a 400 base pair fragment from its 5' end. We labeled
these molecules with .sup.32P by random priming. Then we screened a
human genomic EMBL3 library with the ELAM cDNA probes. We isolated
and characterized a genomic ELAM1 clone from the library and
designated it EL1-07. It includes approximately 15 kb of 5'
flanking sequence including the transcriptional promoter for ELAM1
and approximately 100 base pairs of coding sequence at the 5' end
of the gene. Current knowledge suggests that the relevant control
sequences for induction will be included within the DNA sequence
represented by this phage clone. (Leonardo and Baltimore, 1989.) We
sequenced a region including 840 bp of 5' flanking sequence and 720
bp of the 5' end of the ELAM1 gene, including the first two exons,
the first intron and part of the second intron. We present this
sequence in FIG. 7. The 5' flanking region displays a classical
promoter structure including TATAAA and CAAT sequences. It also
contains the sequence GGGGATTTCC about 95 base pairs upstream from
the presumed start of transcription. This sequence is an
NF-.kappa.B binding sequence identical to that found in the human
.kappa. immunoglobulin (Ig) gene enhancer. NF-.kappa.B is an
inducible DNA binding protein known or suspected to stimulate
transcription of a number of genes relevant to inflammation and the
immune response (such as the immunoglobulins, the interleukin-2
receptor, and .beta.-interferon, among others). It can be activated
by TNF, IL-1, and LPS, the same inducers known to stimulate
production of ELAM1, VCAM1, and ICAM1. (Lenardo and Baltimore,
1989; Osborn et al., 1989.) We have demonstrated that NF-.kappa.B
DNA binding activity is stimulated in endothelial cells by IL-1 and
TNF, and we are currently engaged in defining minimal DNA sequences
necessary for inducible transcription from the ELAM1 promoter, by
transfection of promoter/reporter gene constructs into endothelial
and other cell types.
[0194] We deposited clone EL1-07 under the Budapest Treaty with In
Vitro International, Inc., Linthicum, Md. (USA) on Dec. 7, 1989. It
is identified as: [0195] EL1-07 [0196] Accession Number
IVI-10218.
[0197] We also isolated an EMBL3 genomic clone representing the
VCAM1 gene by probing the previously mentioned EMBL3 human genomic
library with a .sup.32P-labeled 30 base oligomer probe homologous
to the 5' end of the VCAM1 cDNA. We designated this clone VC1-16
and deposited it under the Budapest Treaty with In Vitro
International, Inc., Linthicum, Md. (USA) on Dec. 7, 1989. It is
identified as: [0198] VC1-16 [0199] Accession Number IVI-10217. We
sequenced a region including approximately 300 bp of 5' flanking
sequence and 900 bp of the 5' end of the VCAM1 gene, including the
first exon, the first intron, and part of the second exon. We
present this sequence in FIG. 8. The 5' flanking region has a
classical TATAAA sequence, and two NF-.kappa.B consensus sequences:
AGGGATTTCC on the sense strand from about -63 to -54 from the start
of transcription, and GGGGAAACCC on the reverse complement strand
from about -69 to -78. This sequence will be used for studies
analogous to those proposed for the ELAM1 promoter sequence.
EXAMPLE X
Antibodies Recognizing CDX
[0200] We isolated CDX, a MILA involved in ELAM1-mediated adhesion.
As a first step, we prepared monoclonal antibodies that recognized
an antigen on the leukocyte cell surface and that interfered with
leukocyte-endothelial cell binding. In order to assure that the
antigen that these monoclonals recognized was involved in
ELAM1-mediated adhesion, we tested the monoclonals in systems in
which ELAM1-mediated binding was the exclusive cell-cell binding
pathway.
[0201] 1. Preparation and Analysis of Monoclonal Antibodies Against
CDX
[0202] a. Adhesion Assay
[0203] To identify Moabs that inhibit leukocyte-endothelial cell
binding, we developed an improved assay to detect endothelial
cell-leukocyte adhesion. We performed this assay using HL-60 cells
and HUVECs. It should be clear that one can perform such an assay
using any cell line that expresses a MILA and with any cell line
that expresses an ELAM. In 48-well tissue-culture plates we grew
HUVECs to confluence (8.times.10.sup.4 cells/well). We washed the
cells once with RPMI/1% FCS and added 0.5 ml RPMI/1% FCS with 13
U/ml of IL-1.beta. to each well (except the control wells). We
incubated these cells for 4 hours at 37.degree. C. Just before use,
we washed them once with RPMI/1% FCS. The HL-60 cells we used in
the assay had been labeled overnight with 1 .mu.Ci/ml of
.sup.35S-methionine. We washed these cells once and then
resuspended them in RPMI/1% FCS at 5.times.10.sup.6 cells/ml. We
took 100 .mu.l of the HL-60 cells and incubated them for 30 min at
0.degree. C. with 50 .mu.l of Moab (1 .mu.g/ml). Then we added the
150 .mu.l to each well of HUVECs. We allowed the cells to bind for
10 min at 20.degree. C. and then washed the wells gently once with
RPMI/1% FCS. We filled the wells with RPMI/1% FCS, sealed the
plates, inverted them, and centrifuged them for 2 min at
500.times.g. We removed the media and washed the wells two more
times with PBS.sup.=. (PBS.sup.= is PBS without Ca.sup.++ and
without Mg.sup.++.) We determined the number of HL-60 cells bound
to the HUVECs by solubilizing the cells in each well with 200 .mu.l
of 0.2N NaOH/1% SDS, adding 4.5 ml of scintillant (Ready Protein,
Beckman), and counting with a scintillation counter.
[0204] b. Preparation of Hybridomas
[0205] To make monoclonal antibodies against CDX we prepared
hybridomas in the following manner. We injected BALB C mice with
whole, live HL-60 cells. Initially, each mouse received
2.times.10.sup.7 cells in PBS.sup.= intraperitoneally (IP). We
injected complete Freund's adjuvant intraperitoneally at a
different site 2-24 hours later. We boosted the mice with
2.times.10.sup.7 cells IP every second week for six weeks. Four
days before fusing we injected the mice intravenously with
5.times.10.sup.6 cells and IP with 5.times.10.sup.6 cells.
[0206] We tested immune serum from these animals for the ability to
inhibit binding of the HL-60 cells to IL-1.beta. stimulated HUVECs
by the adhesion assay described above. The immune serum tested
positive after the third boost and we proceeded to produce
hybridomas from the spleen cells of the immunized animals. We
performed fusion of spleen cells and myeloma cells in a manner
standard to the art. (See, Goding, 1983.)
[0207] Using the adhesion assay we described above, we screened the
hybridomas for those producing monoclonal antibodies that inhibited
the binding of HL-60 cells to IL-1.beta.-induced HUVECs. In this
way we identified hybridomas that produced monoclonal antibodies
that recognized CDX. We used five of these hybridomas to produce
ascites fluid. We deposited one of them, designated
SGB.sub.3B.sub.4, under the Budapest Treaty with In Vitro
International, Inc., Linthicum, Md. (USA) on Apr. 25, 1989. It is
identified as: [0208] SGB.sub.3B.sub.4 [0209] Accession number:
IvI-10205
[0210] c. FACS Analysis
[0211] To identify to which cell types our monoclonals bound, we
performed FACS analysis. This involved taking 2.times.10.sup.5
cells, washing them one time with PBS.sup.=, and then blocking Fc
receptors by incubation in 25 .mu.l of RPMI, 1% FCS, 0.1 mg/ml
human IgG, and 0.1% sodium azide for 10 min at 0.degree. C. We then
added antibody (25 .mu.l at 1 .mu.g/ml) and incubated the cells 30
min at 0.degree. C. We centrifuged the cells at 250.times.g for 5
min, washed them two times with Buffer A (PBS.RTM., 5% FCS, 0.1%
azide) and resuspended them in 25 .mu.l Buffer A containing 0.1
mg/ml human IgG. We added fluorescein-conjugated anti-mouse IgG (25
.mu.l at 5 .mu.g/ml in Buffer A (Cappel)) and incubated the mixture
30 min at 0.degree. C. We centrifuged the cells, washed them once
with Buffer A, and resuspended them in 250 .mu.l Buffer A. Then we
analyzed them on a Becton-Dickinson FACStar Cell Sorter.
[0212] We performed cell binding studies with the ELAM1-expressing
COS cells essentially as described for the HL-60 cell-HUVEC
adhesion assay.
[0213] 2. Demonstration That Hybridoma SGB.sub.3B Produced
Monoclonal Antibodies That Recognize CDX
[0214] We have developed several lines of evidence that demonstrate
that monoclonals from hybridoma SGB B specifically recognize a MILA
involved in ELAM1-mediated binding, specifically, CDX.
[0215] First, the .alpha.-CDX antibodies should inhibit binding of
cells expressing CDX to ELAM1-expressing cells. Using the adhesion
assay, we showed that these monoclonals do indeed inhibit the
binding of HL-60 cells and PMNs to IL-1.beta.-induced HUVECs and
ELAM1-expressing COS 7 cells. In the presence of 60.3, a monoclonal
antibody against the .beta..sub.2 integrin chain, the only binding
pathway for HL-60 cells and PMNs that is utilized in
ELAM1-expressing COS 7 cells is ELAM1 itself. Therefore, antibody
inhibition of cell-cell adhesion in this system must be through the
ELAM1 pathway via CDX.
[0216] Second, .alpha.-CDX monoclonals should recognize those cells
that bind to ELAM1-expressing cells in an adhesion assay, but
should not recognize those cells that do not bind to ELAM1 in this
assay. Using FACS analysis, we determined the binding pattern of
our Moabs. These monoclonals bound to the following cell types:
HL-60, U937, HT-29, THP-1, SW620, SW948, SW1417, monocytes,
eosinophils, and PMNs. They did not bind to these cells: RAJI,
DAUDI, RAMOS, HeLa, or JY. (We isolated the non-transformed cells
by fractionating peripheral blood leukocytes.) This binding pattern
precisely parallels the binding of these cells to ELAM1-expressing
COS 7 cells and to rsELAM1-coated plates.
[0217] Third, .alpha.-CDX monoclonals should exhibit a different
recognition pattern than monoclonals against other leukocyte
cell-surface antigens, such as LFA-1, LFA-3, CD44, ICAM1 and CD4.
In fact, no other monoclonal of which we are aware exhibits the
same cell-recognition pattern as our antibodies.
[0218] Fourth, and most convincing, using these MoAbs we cloned a
gene that can confer ELAM1 binding activity in cells that otherwise
do not bind to ELAM1.
[0219] In sum, it is apparent that the monoclonals produced by
hybridoma SGB.sub.3B.sub.4, and by other hybridomas we isolated,
recognize CDX. Consequently, we used these monoclonals to isolate
CDX itself.
EXAMPLE XI
Isolation of CDX
[0220] 1. Iodination of HL-60 Cell Surface Proteins
[0221] We washed 1.times.10.sup.7 HL-60 cells three times with
PBS.dbd., resuspended them in 0.5 ml PBS.sup.= and added them to a
tube coated with 50 .mu.g
1,3,4,6-tetrachloro-3.alpha.,6.alpha.-diphenylglycouril (Sigma
Chemical Co.). To this we added 1 mCi of .sup.125I. We incubated
the mixture for 30 min at 0.degree. C. We transferred labeled cells
to a tube containing 10 ml of RPMI/10% FCS and centrifuged them at
1000.times.g for 5 min. Then we washed them first with another 10
ml of RPMI/10% FCS and second with 2 ml of PBS.RTM..
(Alternatively, we have labeled the cells metabolically with
.sup.35S-methionine or 5-cysteine.) We lysed the cells by addition
of 1.0 ml PBS.sup.= containing 1% NP40, 2 mM PMSF, 1 mM EDTA,
soybean Trypsin inhibitor (50 mg/ml), and Leupeptin (1 mM) (Sigma
Chemical Co.). Then we incubated them for 30 min at 0.degree. C. We
centrifuged the lysate for 10 min at 10,000.times.g to remove
particulate matter. We precleared the supernatant containing
labeled solubilized membrane proteins with 10 .mu.g of rabbit
anti-mouse IgM (Jackson Immuno-Research Labs) and 50 .mu.l of
Protein A sepharose (Zymed, 2 mg Protein A/ml) for 2 hours at
0.degree. C. We stored the lysate at 4.degree. C.
[0222] 2. Immunoprecipitation of CDX
[0223] We purified CDX away from the other labeled proteins using
the Moabs to immunoprecipitate it. We performed the
immunoprecipitation as follows:
[0224] We incubated precleared lysate (50-100 .mu.l) with 10.lamda.
of ARX beads for 2 hours at 4.degree. C. We washed the sepharose
four times with 2 ml PBS.sup.= containing 0.75% NP40, 0.2% DOC, and
1 mM EDTA. Then we resuspended the ARX beads in non-reducing SDS
sample buffer. We heated the sample for 10 min at 85.degree. C. and
removed the supernatant. To this we added B-ME to 5%, heated for 5
min, and separated the molecules on a 10% SDS polyacrylamide gel.
We dried the gel and autoradiographed it.
[0225] CDX appeared on the autoradiograph as a single, diffuse band
with molecular weight of approximately 150 kD.
EXAMPLE XII
Isolation and Characterization of Clone 7.2 and Clone 1
[0226] We prepared two cDNA libraries in the pCDM8 vector from two
types of CDX-expressing cells, HL-60 cells and U937 cells. We
isolated the mRNA from these cells and reverse-transcribed it into
cDNA using techniques well known to the art. (Gubler and Hoffman,
1983.) Using standard procedures, we ligated double stranded cDNA
to a NotI-BstXI linker/adaptor having the following sequence:
TABLE-US-00002 5' GCG GCC GCT TTA GAG CAC A 3' 3' CGC CGG CGA AAT
CTC 5'
We then size-selected the cDNA on a 4.2 ml 5-20% potassium acetate
gradient, 2 mM EDTA, 1 .mu.g/ml ethidium bromide, in a BECKMAN SW60
Rotor for 3 hours at 50,000 rpm at 22.degree. C. according to the
protocols of Brian Seed. (See also Maniatis, 1982, p. 278.) We
pooled the cDNA fragments of greater than 500 base pairs. Then we
prepared the vector, PCDM8 (a gift from Brian Seed). We digested
this plasmid with BstXI. To remove the 400 base pair stuffer
fragment we centrifuged the mixture on a potassium acetate
gradient, as above, and isolated the large fragment. We further
purified this fragment by agarose gel electrophoresis, and then
ligated the cDNA to the vector.
[0227] We then prepared an enriched cDNA library by first creating
a .sup.32P-labeled cDNA probe from 1 microgram of HL-60 poly A+
mRNA, then subtracting non-CDX related cDNA sequences from the
probe by hybridizing with 30 micrograms of poly A+ mRNA from HeLa
cells, which do not express CDX. (See, Davis, 1986.) We used the
subtracted probe to screen the pCDM8 cDNA library and thus created
an enriched sublibrary from HL-60 cells in E. coli MC1061 P3. We
grew about 2100 clones in twenty-two 96-well plates. A U937
enriched sublibrary was prepared in a similar manner, and 1400
clones were obtained.
[0228] We divided the colonies from our HL-60 enriched library into
22 pools for transfection of COS 7 cells by spheroplast fusion.
(Sandri-Goldin et al. 1981.) We assayed transfected COS 7 cells for
ELAM1-binding activity by panning with .alpha.-CDX monoclonal
antibodies from hybridoma SGC2E.sub.5 (an antibody similar in
function to SGB.sub.3B.sub.4) according to the method of Seed and
Aruffo (1987). (See also Aruffo and Seed, 1987 and Wysocki and
Sato, 1978). Pool #7 assayed positive, yielding two clones with a
2.1 kb cDNA insert. These were designated clones 7.1 and 7.2.
[0229] We obtained the DNA sequence of clone 7.2 by the Maxam and
Gilbert technique (Maxim and Gilbert, 1980) from CDX pCDM8 clone
7.2 and from a portion of the 7.2 insert subcloned into the
sequencing vector, pNN11. The latter plasmid was designated pSQ219.
The DNA sequence obtained is set forth in FIG. 9.
[0230] We deposited a culture containing the plasmid CDX pCDM8
clone 7.2 under the Budapest Treaty with In Vitro International,
Inc., 611 P. Hammonds Ferry Rd., Linthicum, Md. 21090 (USA) on Apr.
26, 1990. The deposit is identified as: [0231] CDX pCDM8/E. coli
MC1061 P3 [0232] Accession Number IVI-10242
[0233] We also performed a Northern blot on mRNA from HL-60 cells
and probed it with clone 7.2. Clone 7.2 hybridized to three mRNA
species, two prominent bands at 6.0 kb and 2.4 kb and another band
at 3.0 kb. Clone 7.2, a cDNA of 2.1 kb, is not large enough to be a
full length cDNA from the 3.0 kb and 6.0 kb species. Therefore, in
order to identify DNA sequences for these messages, we probed the
enriched cDNA sublibrary from both U937 and HL-60 cells with an
oligonucleotide derived from clone 7.2. We isolated several long
inserts from the HL-60 library, transfected them into COS 7 cells,
and selected clones that bound to ELAM1 and .alpha.-CDX. In this
way we identified a 2.9 kb insert that could have come from the 3.0
kb message. We called it CDX clone 1.
[0234] We determined the DNA sequence of CDX clone 1 by the Maxam
and Gilbert technique. The DNA sequence obtained is set forth in
FIG. 10.
[0235] We deposited a culture containing the plasmid CDX clone 1
under the Budapest Treaty with In Vitro International, Inc., 611 P.
Hammonds Ferry Rd., Linthicum, Md. 21090 (USA) on Oct. 11, 1990.
The deposit is identified as: [0236] CDX clone 1 pCDM8/E. coli
MC1061 P3 [0237] Accession Number IVI-10255.
[0238] We transfected clone 7.2 and clone 1 into COS 7 and CHO
cells. At 48 hours after transfection these cells expressed a
glycoprotein on their cell surfaces to which fluorescently labelled
.alpha.-CDX antibodies bound, as assayed by FACS. These cell
surface proteins could be labeled with .sup.125I and
immunoprecipitated with .alpha.-CDX Moabs. We designated the
protein isolated from COS 7 cells, Pseudo-X and from CHO cells,
Pseudo-X.sub.2. On SDS polyacrylamide gels, Pseudo-X and
Pseudo-X.sub.2 were approximately 130 kD and 140 kD,
respectively.
[0239] The transfected COS cells also formed rosettes around
Sepharose beads coated with recombinant soluble ELAM1 (rsELAM1);
and the rosetting was cation dependent and was inhibited by both
BB11 (anti-ELAM1 antibody) and .alpha.-CDX. COS cells and CHO cells
transfected with PCDM8 alone (without the inserted clone) did not
rosette rsELAM1 beads. Also, the COS and CHO cells transfected with
clone 7.2 did not rosette to beads coated with bovine serum
albumin.
[0240] We further characterized clone 7.2 and clone 1 by DNA
sequence analysis and enzyme assays. Clone 1 encodes a polypeptide
of 530 amino acids (encoded by nucleotides. 174-1763 of FIG. 2).
Clone 7.2 encodes a 405-amino acid polypeptide (encoded by
nucleotides 66-1280 in FIG. 1). Using UWGCG Sequence Analysis
Software Package (version 6.1, August 1989), we searched the NBRF
Protein database (release 23, December 1989) using the program
FASTA for homology to other proteins. We also searched GenBank
(release 63, March 1990) and EMBL (release 19, May 1989) using
TFASTA. In these searches we found short regions (e.g., about 23
amino acids) of homology to certain viral envelope proteins
including Herpes simplex virus type 1, Dengue virus, yellow fever
and other flaviviruses. In general the homology to known proteins
was low, and we conclude that the polypeptides are novel.
[0241] The portion of the nucleotide sequence of clone 7.2 from
nucleotide 9 to nucleotide 2162 (FIG. 9) is identical to the
portion of the sequence of clone 1 from nucleotide 492 to
nucleotide 2645 (FIG. 10). The first methionine of protein 7.2
corresponds to the methionine at amino acid 126 of protein 1. One
explanation of this homology is that the two inserts represent
different transcripts from the same DNA segment.
[0242] As we stated earlier, these clones do not code for CDX,
Pseudo-X or Pseudo-X.sub.2--the polypeptides they encode are not
the correct, size. Rather, the evidence strongly supports the
conclusion that clone 7.2 and clone 1 encode 1,3-fucosyl
transferases that glycosylate other proteins, such as CDX, Pseudo-X
and Pseudo-X.sub.2, in a way that makes them "visible" (i.e.,
recognized by or able to bind to) ELAM1 or .alpha.-CDX. First, the
DNA sequences of clone 1 and clone 7.2 share several structural
features with the DNA sequences of known glycosyl transferases. For
example, genes encoding known glycosyl transferases commonly have
consecutive methionine start sites and are capable of producing
more than one mRNA transcript. As mentioned above, we have
identified three mRNA transcripts that hybridize to clone 7.2, and
clone 1 contains two codons that can serve as transcription start
signals. Also, like known glycosyl transferases, the clones have
multiple SP1 enhancer sites. The nucleotide sequences for these
sites are GGGCGG or CCGCCC; clone 1 has five such sites. Also, like
known glycosyl transferases, clones 7.2 and 1 are rich in guanine
(G) and cytosine (C). For example, clone 1 is 75% GC rich in the 5'
region of the gene and 60% GC rich in the 3' region of the gene.
Glycosyl transferases in addition are typically class II membrane
proteins, in which the membrane-spanning domain is near the amino
terminus and the extracellular portion is near the carboxy
terminus. Clone 1 and clone 7.2 encode a polypeptide having a
hydrophobic region near the amino terminus. Glycosyl transferases
also tend to have molecular weights between 40 kD to 60 kD; clone 1
encodes a polypeptide of about 59 kD and clone 7.2 encodes a
polypeptide of about 46 kD. Finally, known glycosyl transferases
usually have one to three N-glycosylation sites; clone 1 and clone
7.2 both encode two such sites.
[0243] Second, enzyme assays performed on extracts from CHO cells
transfected with clone 7.2 revealed the presence of fucosyl
transferases not expressed in untransformed cells. The assays
tested the ability of the enzyme to link radioactively labelled
fucose to an acceptor molecule. We performed the assays as
follows.
[0244] We prepared assay samples containing 10 .mu.l enzyme, 8
.mu.l cocktail and 2 .mu.l 10.times. acceptor. We prepared the
enzyme by isolating about 1.5 million CHO cells transfected with
clone 7.2 and lysing them by sonication for 15 seconds in 150 .mu.l
ice-cold 1% Triton X-100 in water. The cocktail contained 75 .mu.M
.sup.14C-GDP fucose, 100 mM ATP, 500 mM L-fucose, 1 M MnCl.sub.2
and 1 M cacodylate at pH 6.2. 10.times. acceptor contained,
variously, 200 mM LacNAc, Lac-N-biose, or lactose, 250 mM
phenyl-.beta.-D-galactoside, or 50 mM 2'-fucosyllactose. We
incubated the assay samples for 1 hour at 37.degree. C. We stopped
the reaction by addition of 20 .mu.l ethanol. We diluted the sample
with 560 .mu.l water and centrifuged in an EPPENDORF centrifuge for
5 minutes at high speed.
[0245] We had prepared a DOWEX 1.times.2-400 column (Sigma Chemical
Co.) to separate the unconverted .sup.14C fucose-GDP from the
converted. We loaded the matrix into a large column and washed it
with 10 volumes of 1N NaOH, followed by 5 volumes of water,
followed by 10 volumes of 5% concentrated formic acid. Then we
repeated this wash cycle. We used this material to create small
columns of 0.4 ml. We prepared the small columns for use by washing
them with 10 volumes of water.
[0246] We loaded 200 .mu.l of the sample onto the small column,
collected the eluate, rinsed with 2 ml water and collected it into
the eluate. We determined the radioactivity of this eluate by
scintillation counting.
[0247] The results of this assay demonstrated that the induced
enzyme is a 1,3-fucosyl transferase. (See Table 1.) The enzyme
linked fucose to LacNAc, 2'-fucosyllactose and lactose, acceptors
having GlcNAc or glucose moieties with free 3' hydroxyls. It did
not link fucose to LacNBiose, whose GlcNAc moiety does not have a
free 3' hydroxyl, or phenyl-.beta.-D-galactoside, the negative
control acceptor. Control samples from untransfected cells showed
only insignificant linking of fucose to these acceptors.
TABLE-US-00003 TABLE 1 Efficiency of Fucosylation picomoles
Acceptor mg Total protein hr LacNAc 1110 Lac-N-Biose 76
2'-Fucosyllactose 151 Lactose 290 Ph.beta.Dgal Not detectable
[The enzyme was freshly produced from transfected CHO cells.]
[0248] Therefore, both genetic and enzymatic evidence indicate that
clone 7.2 and clone 1 encode 1,3-fucosyl transferases.
EXAMPLE XIII
Antibodies Recognizing MILAs For VCAM1
[0249] Polyclonal antisera were obtained from three mice that had
been immunized with whole JURKAT cells. The serum from one mouse
completely inhibited both RAMOS and JURKAT binding to 4
hour-induced HUVECs at room temperature. The sera from the two
other mice completely inhibited RAMOS but only partially inhibited
JURKAT binding under the same conditions. These data indicate that
RAMOS and JURKAT share a MILA, and that JURKAT exhibits at least
one other MILA not shared by RAMOS.
[0250] To prepare Moabs to lymphocyte MILAs, we immunized mice
against whole live RAMOS and JURKAT cells and performed fusion of
spleen cells from JURKAT-immunized mice and myeloma cells in the
manner described in Example VIII, above. We are screening the
resulting hybridomas by the method described in Example VII, which
we used successfully to obtain monoclonal antibodies to CDX. To
date we have screened the conditioned medium from about 260
hybridomas for inhibition of RAMOS adhesion to HUVECs treated with
TNF for 24 hours. About 25 hybridomas have shown consistent partial
inhibition of adhesion, and these are currently being subcloned for
re-screening. Such antibodies may be used to both isolate and clone
lymphocyte MILAs.
EXAMPLE XIV
Evidence that VLA4 is a VCAM1 Ligand
[0251] We and other colleagues have performed several studies that
demonstrate that VLA4 is a VCAM1 ligand and that VLA4 has separate
binding sites for VCAM1 and fibronectin.
[0252] First, we showed that monoclonal antibodies against the
subunits of VLA4 inhibited the attachment of VLA4-expressing cells
to activated HUVECs and to COS cells transfected with VCAM1. VLA4
is composed of the subunits .beta..sub.1 and .alpha..sup.4.
(Hemler, 1988.) We found that a monoclonal antibody against
.beta..sub.1, designated B1E11, and goat anti-.beta..sub.1
heteroantiserum completely inhibited the adhesion of RAMOS cells to
activated HUVECs and transfected COS cells. A control antibody did
not inhibit adhesion. Furthermore, a monoclonal antibody against
the .alpha..sup.4 subunit, designated HP2/1, also blocked
attachment of RAMOS to these cells. Similarly, these antibodies
inhibited the attachment of the VLA4-expressing T lymphoblastoid
cell line HPB-ALL.
[0253] Next, we showed that transfecting cells that do not
ordinarily express VLA4 with .alpha..sup.4 enabled them to bind to
VCAM1-expressing cells. We transfected two sets of K-562
erythroleukemic cells. One set was transfected with a cDNA coding
for .alpha..sup.4. (Takada et al., 1989.) The other was transfected
with .alpha..sup.2, which is not part of VLA4. (Takada and Hemler,
1989.) We showed that K-562 cells transfected with .alpha..sup.4
were now able to bind with a monolayer of VCAM1-transfected COS
cells or TNF-activated HUVECs, but parent K-562 cells and K-562
.alpha..sup.2-transfected cells were not. In addition, monoclonal
antibodies against .alpha..sup.4 or .beta..sub.1 abolished the
adhesion of .alpha..sup.4-transfected K-562 cells (that normally
express the subunit) to these VCAM1-expressing cells.
[0254] Recent studies have shown that VLA4 mediates cell attachment
to human plasma fibronectin (FN) through the FN CS-1 site. (Wayner
et al., 1989.) We have shown that the VLA4 binding site for VCAM1
is different than its binding site for FN. First, we found that
preincubation of RAMOS cells or .alpha..sup.4-transfected K-652
cells with FN-40 (a soluble FN fragment) inhibited their binding
with FN-40, but not with VCAM1-transfected COS cells or TNF.alpha.
activated HUVECs. Second, we found that a monoclonal against VLA4,
HP1/3, inhibited the binding of these cells to transfected COS
cells or activated HUVECs, but not to FN-40.
EXAMPLE XV
Inhibitor Screening
[0255] One can use ELAMs and their ligands in three basic adhesion
assays to screen for potential inhibitors of adhesion, such as
synthetic organic chemicals, natural fermentation products,
peptides, etc.:
[0256] 1. Cell-Cell Adhesion Assays
[0257] A first assay would test the ability of molecules to inhibit
cell-cell adhesion. One could perform this assay in 96-well
microtiter plates. First, one creates a cell line that stably
expresses an ELAM, for example, as described in Example V. Then one
plates out these cells and adds HL-60 cells. Inhibitors are
identified by their ability to inhibit HL-60 binding to the
ELAM-expressing cells. One would perform an assay exactly as
described for screening for monoclonal antibodies to the ELAM
ligand.
[0258] 2. Cell-Adhesion Protein Assays
[0259] A second assay would test the ability of a small molecule to
inhibit cell binding to ELAM itself. We have developed such an
assay with rsELAM1 which works in 96 well microtiter plates. These
plates, made of bacteriologic plastic (e.g. Linbro/Titertek
#76-232-05.RTM.), are incubated with 0.5 .mu.g per well of rsELAM1
in 50 .mu.l of 15 mM sodium carbonate/35 mM sodium bicarbonate, pH
9.2, overnight at 40. The plates are then blocked for one hour at
room temperature with PBS containing 10 mg/ml of bovine serum
albumin, and then adhesion assays performed as described in Example
VIII using, e.g., HL-60 cells, 2.times.10.sup.6/ml, 50 .mu.l per
well. Under these conditions HL-60 cells bind well to rsELAM1,
providing a convenient microassay for screening. One would identify
inhibitors by their ability to inhibit HL-60 binding to the plate.
Alternatively, one could use an ELAM ligand in this assay, using as
the probe a cell line that stably expresses an ELAM.
[0260] Another alternative assay in this category would examine the
binding of a soluble ELAM or ELAM ligand to monolayers of cells
stably expressing an ELAM ligand or ELAM, respectively. The soluble
molecule would be labeled with a reporter group (e.g.,
radioactivity, fluorescent probe, enzyme, etc.)
[0261] 3. Adhesion Protein-Adhesion Protein Assays
[0262] This assay tests the ability of a small molecule to inhibit
the binding of an ELAM to its ligand. One of the two molecules in
soluble form, e.g., a soluble ELAM, is immobilized in the wells of
a 96-well microtiter plate, and adhesion is measured by binding of
the other member of the pair, e.g., an ELAM ligand labeled with a
reporter group.
[0263] In each of these three assays, one detects inhibitors by
their ability to inhibit adhesion.
EXAMPLE XVI
VCAM1/Immunoglobulin Construct
[0264] We have prepared a DNA sequence which, on expression,
produces an rsVCAM1/immunoglobulin fusion protein. The DNA sequence
contains, from 5' to 3', VCAM1 domains 1-3 and the constant region
of an IgG heavy chain gene.
[0265] We produced a DNA fragment containing the VCAM1 domains 1-3
through nucleotide 1035 of FIG. 3 by polymerase chain reaction
(PCR). (Sambrook et al., 1989) The 3'-5' primer had the sequence 5'
GA GCT CGA GGC CGC ACC ATG CCT GGG AAG ATG. It is complementary to
nucleotides 100-114 in FIG. 3 and contains the VCAM1 initiation
codon and recognition sites for XhoI and NotI. The 5'-3' primer had
the sequence 5' CT AGC TAG CGC GTT TTA CTT CAC. It is complementary
to nucleotides 1016-1035 in FIG. 3, at the end of domain 3, and
contains an NheI recognition site. We used these primers to amplify
a segment from a plasmid containing VCAM1 coding region of AM pcDM8
clone 41. The product of this process was a DNA sequence encoding
VCAM1 domains 1-3. We digested this DNA fragment with XhoI and NheI
and inserted it into pAB53, which we made as follows.
[0266] We digested pJOD-s (Example VIII) with SalI and inserted a
cDNA sequence encoding human rsCD4. We called this plasmid
pJOD-rsT4. We partially digested pJOD-rsT4 with PvuII and SphI to
delete the fragment containing the two SV40 enhancer repeats in the
SV40 promoter which control transcription of the DHFR cDNA. We
religated the plasmid and designated it pJOD-rsT4 delta E. Then we
digested pJOD-rsT4 delta E with NheI and NotI and inserted two DNA
fragments: first, an NheI-HindIII linker containing a 5' mRNA
splice site and second, a DNA fragment encoding the constant region
of an IgG heavy chain gene. We obtained these fragments as
follows.
[0267] We synthesized an NheI-HindIII linker having the following
sequence: TABLE-US-00004 5' splice 5' CTA GCT TTC CAA GGT GAG TCC
TA 3' 3' GA AAG GTT CCA CTC AGG ATT CGA 5'
[0268] The DNA sequence of an IgG heavy chain gene is described in
Ellison et al. (1982). We isolated a fragment of this gene from an
EMBL3 human genomic library (Example VIII) using an oligonucleotide
probe. We digested the fragment with HindIII and NotI and isolated
the fragment which included the constant heavy domains and the
associated introns.
[0269] We ligated these two fragments into PJOD-rsT4 delta E and
called the resulting plasmid pAB53. We digested pAB53 with XhoI and
NheI to delete the rsT4 coding region. We inserted in its place the
XhoI-NheI fragment encoding VCAM1 domains 1-3. We called this
plasmid VCAM1-IgG.sub.1.
[0270] An rsVCAM1/IgG fusion protein is expressed using this
plasmid. The plasmid is transfected into CHO cells for stable
expression. After transcription of this gene, the mRNA is spliced
to remove the introns and upon translation, the cell produces
rsVCAM-IgG fusion protein.
EXAMPLE XVII
Inhibiting VCAM1 Expression with an Antisense Nucleic Acid
[0271] We describe here an antisense nucleic acid against VCAM1 and
a method for testing its ability to inhibit VCAM1 expression in
induced HUVECs. An effective nucleic acid sequence for an antisense
nucleic acid is one that is complementary to the coding region of
the mRNA and, more particularly, to either the initiation codon,
AUG, or the splice sites. (Marcus-Sekura, 1988.) Also, oligomers of
about 15 nucleotides are most preferred. Thus, an effective
antisense nucleic acid against VCAM1 is an oligomer with the DNA
sequence 5' CCC AGG CAT TTT AAG. This would bind to nucleotides
94-108 of FIG. 3 (CAT is the antisense initiation codon.) This DNA
sequence is synthesized, for example, by an automated DNA
synthesizer.
[0272] The ability of this antisense nucleic acid to inhibit VCAM1
expression is tested as follows. HUVECs are grown to confluence as
in Example V except that the serum used for cell growth would be
heat inactivated or 30 min. at 60.degree. to inactivate nucleases.
Cells are preincubated with the oligomers at concentrations between
10 .mu.M and 100 .mu.M, most preferably the highest concentration
having no effect on cell viability, for four to forty-eight hours.
These ranges are required for effective inhibition. (Marcus-Sekura,
1988; Becker et al., 1989.) The HUVECs are then treated with 10
ng/ml TNF to induce VCAM1. About four hours later the presence of
VCAM1 on the surface of the cells is tested by the adhesion
assay.
EXAMPLE XVIII
A Hammerhead Ribozyme Which Recognizes VCAM1 mRNA
[0273] A hammerhead-type ribozyme which recognizes VCAM1 mRNA is
prepared according to the rules of Haselhoff and Gerlach (1988) as
follows. First, a cleavage site on the target mRNA is identified.
Hammerhead ribozymes cleave after the sequence 5' GUX, where X is
any nucleotide. The first instance of this sequence in the coding
region of VCAM1 mRNA is the sixth codon: 5' AUG CCU GGG AAG AUG GUC
GUG AUC CUU. An appropriate recognition sequence includes about six
nucleotides of the 5' and 3' regions flanking the cleavage site. An
eighteen-base recognition sequence which contains the cleavage site
is 5' AAG AUG GUC GUG AUC CUU.
[0274] Then, one designs an RNA sequence for the ribozyme
containing the recognition sequence and a sequence for the
catalytic "hammerhead." Such a sequence is 5' AAG GAU CAC
[CUGAUGAGUCCGUGAGGACGAA] AC CAU CUU. The sequence in brackets
generates the catalytic "hammerhead" and the 5' and 3' flanking
sequences are complementary to and bind to the recognition
sequence. In a similar way, one can also design shorter recognition
sequences or those for other cleavage sites in VCAM1 mRNA or the
other ELAM or ELAM ligand mRNAs.
EXAMPLE XIX
Anti-Idiotypic Antibodies Recognizing ELAM1 Ligands
[0275] We have prepared anti-idiotypic antibodies against
anti-ELAM1 antibodies that bind to the ligand of ELAM1 on HL-60
cells. We immunized rabbits with protein-A-purified CDB.BB11.BC6
monoclonal (Example V) emulsified 1:1 in complete Freund's
adjuvant. Twenty-six days after immunization we bled the rabbits
and analyzed the anti-sera for specific antibodies using FACS. We
incubated the antibody preparation with either HL-60 cells, which
express a ligand for ELAM1, or RAMOS cells, which do not. We found
that this antibody preparation specifically bound to the HL-60
cells and not to the RAMOS cells, indicating that it contained
antibodies that recognize the ELAM1 ligand. Control anti-serum did
not react with either cell line.
EXAMPLE XX
Evidence of a New ELAM
[0276] The binding of U937 cells (which are monocyte-like) to
induced HUVECs is not blocked by specific Moabs to the ELAM1,
VCAM1, and/or ICAM1 pathways. U937 binding is blocked, however, by
a monoclonal antibody to CD29, the .beta..sub.1 integrin subunit.
This leads us to postulate the existence of a new adhesion molecule
on HUVECs that interacts with leukocytes via a .beta..sub.1
integrin. The new molecule is induced with a time-course similar to
VCAM1, remaining at maximal levels 48 hours after induction. We
have generated a subtracted library from 48-hour TNF-treated
HUVECS, using the methods previously described for the 2.5-hour
IL-1 induced HUVEC subtracted sublibrary. We are attempting to
clone the new molecule using the direct expression protocol
described previously.
[0277] While we have described herein a number of embodiments of
this invention, it is apparent that one of skill in the art could
alter our procedures to provide other embodiments that utilize the
processes and compositions of this invention. Therefore, one will
appreciate that the scope of this invention is to be defined by the
claims appended hereto rather than by the specific embodiments that
we have presented by way of example.
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Sequence CWU 1
1
28 1 3863 DNA Homo Sapien CDS (141)...(1973) 1 ttcacatcaa
aactcctata ctgacctgag acagaggcag cagtgatacc cacctgagag 60
atcctgtgtt tgaacaactg cttcccaaaa cggaaagtat ttcaagccta aacctttggg
120 tgaaaagaac tcttgaagtc atg att gct tca cag ttt ctc tca gct ctc
act 173 Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr 1 5 10 ttg gtg
ctt ctc att aaa gag agt gga gcc tgg tct tac aac acc tcc 221 Leu Val
Leu Leu Ile Lys Glu Ser Gly Ala Trp Ser Tyr Asn Thr Ser 15 20 25
acg gaa gct atg act tat gat gag gcc agt gct tat tgt cag caa agg 269
Thr Glu Ala Met Thr Tyr Asp Glu Ala Ser Ala Tyr Cys Gln Gln Arg 30
35 40 tac aca cac ctg gtt gca att caa aac aaa gaa gag att gag tac
cta 317 Tyr Thr His Leu Val Ala Ile Gln Asn Lys Glu Glu Ile Glu Tyr
Leu 45 50 55 aac tcc ata ttg agc tat tca cca agt tat tac tgg att
gga atc aga 365 Asn Ser Ile Leu Ser Tyr Ser Pro Ser Tyr Tyr Trp Ile
Gly Ile Arg 60 65 70 75 aaa gtc aac aat gtg tgg gtc tgg gta gga acc
cag aaa cct ctg aca 413 Lys Val Asn Asn Val Trp Val Trp Val Gly Thr
Gln Lys Pro Leu Thr 80 85 90 gaa gaa gcc aag aac tgg gct cca ggt
gaa ccc aac aat agg caa aaa 461 Glu Glu Ala Lys Asn Trp Ala Pro Gly
Glu Pro Asn Asn Arg Gln Lys 95 100 105 gat gag gac tgc gtg gag atc
tac atc aag aga gaa aaa gat gtg ggc 509 Asp Glu Asp Cys Val Glu Ile
Tyr Ile Lys Arg Glu Lys Asp Val Gly 110 115 120 atg tgg aat gat gag
agg tgc agc aag aag aag ctt gcc cta tgc tac 557 Met Trp Asn Asp Glu
Arg Cys Ser Lys Lys Lys Leu Ala Leu Cys Tyr 125 130 135 aca gct gcc
tgt acc aat aca tcc tgc agt ggc cac ggt gaa tgt gta 605 Thr Ala Ala
Cys Thr Asn Thr Ser Cys Ser Gly His Gly Glu Cys Val 140 145 150 155
gag acc atc aat aat tac act tgc aag tgt gac cct ggc ttc agt gga 653
Glu Thr Ile Asn Asn Tyr Thr Cys Lys Cys Asp Pro Gly Phe Ser Gly 160
165 170 ctc aag tgt gag caa att gtg aac tgt aca gcc ctg gaa tcc cct
gag 701 Leu Lys Cys Glu Gln Ile Val Asn Cys Thr Ala Leu Glu Ser Pro
Glu 175 180 185 cat gga agc ctg gtt tgc agt cac cca ctg gga aac ttc
agc tac aat 749 His Gly Ser Leu Val Cys Ser His Pro Leu Gly Asn Phe
Ser Tyr Asn 190 195 200 tct tcc tgc tct atc agc tgt gat agg ggt tac
ctg cca agc agc atg 797 Ser Ser Cys Ser Ile Ser Cys Asp Arg Gly Tyr
Leu Pro Ser Ser Met 205 210 215 gag acc atg cag tgt atg tcc tct gga
gaa tgg agt gct cct att cca 845 Glu Thr Met Gln Cys Met Ser Ser Gly
Glu Trp Ser Ala Pro Ile Pro 220 225 230 235 gcc tgc aat gtg gtt gag
tgt gat gct gtg aca aat cca gcc aat ggg 893 Ala Cys Asn Val Val Glu
Cys Asp Ala Val Thr Asn Pro Ala Asn Gly 240 245 250 ttc gtg gaa tgt
ttc caa aac cct gga agc ttc cca tgg aac aca acc 941 Phe Val Glu Cys
Phe Gln Asn Pro Gly Ser Phe Pro Trp Asn Thr Thr 255 260 265 tgt aca
ttt gac tgt gaa gaa gga ttt gaa cta atg gga gcc cag agc 989 Cys Thr
Phe Asp Cys Glu Glu Gly Phe Glu Leu Met Gly Ala Gln Ser 270 275 280
ctt cag tgt acc tca tct ggg aat tgg gac aac gag aag cca acg tgt
1037 Leu Gln Cys Thr Ser Ser Gly Asn Trp Asp Asn Glu Lys Pro Thr
Cys 285 290 295 aaa gct gtg aca tgc agg gcc gtc cgc cag cct cag aat
ggc tct gtg 1085 Lys Ala Val Thr Cys Arg Ala Val Arg Gln Pro Gln
Asn Gly Ser Val 300 305 310 315 agg tgc agc cat tcc cct gct gga gag
ttc acc ttc aaa tca tcc tgc 1133 Arg Cys Ser His Ser Pro Ala Gly
Glu Phe Thr Phe Lys Ser Ser Cys 320 325 330 aac ttc acc tgt gag gaa
ggc ttc atg ttg cag gga cca gcc cag gtt 1181 Asn Phe Thr Cys Glu
Glu Gly Phe Met Leu Gln Gly Pro Ala Gln Val 335 340 345 gaa tgc acc
act caa ggg cag tgg aca cag caa atc cca gtt tgt gaa 1229 Glu Cys
Thr Thr Gln Gly Gln Trp Thr Gln Gln Ile Pro Val Cys Glu 350 355 360
gct ttc cag tgc aca gcc ttg tcc aac ccc gag cga ggc tac atg aat
1277 Ala Phe Gln Cys Thr Ala Leu Ser Asn Pro Glu Arg Gly Tyr Met
Asn 365 370 375 tgt ctt cct agt gct tct ggc agt ttc cgt tat ggg tcc
agc tgt gag 1325 Cys Leu Pro Ser Ala Ser Gly Ser Phe Arg Tyr Gly
Ser Ser Cys Glu 380 385 390 395 ttc tcc tgt gag cag ggt ttt gtg ttg
aag gga tcc aaa agg ctc caa 1373 Phe Ser Cys Glu Gln Gly Phe Val
Leu Lys Gly Ser Lys Arg Leu Gln 400 405 410 tgt ggc ccc aca ggg gag
tgg gac aac gag aag ccc aca tgt gaa gct 1421 Cys Gly Pro Thr Gly
Glu Trp Asp Asn Glu Lys Pro Thr Cys Glu Ala 415 420 425 gtg aga tgc
gat gct gtc cac cag ccc ccg aag ggt ttg gtg agg tgt 1469 Val Arg
Cys Asp Ala Val His Gln Pro Pro Lys Gly Leu Val Arg Cys 430 435 440
gct cat tcc cct att gga gaa ttc acc tac aag tcc tct tgt gcc ttc
1517 Ala His Ser Pro Ile Gly Glu Phe Thr Tyr Lys Ser Ser Cys Ala
Phe 445 450 455 agc tgt gag gag gga ttt gaa tta cat gga tca act caa
ctt gag tgc 1565 Ser Cys Glu Glu Gly Phe Glu Leu His Gly Ser Thr
Gln Leu Glu Cys 460 465 470 475 aca tct cag gga caa tgg aca gaa gag
gtt cct tcc tgc caa gtg gta 1613 Thr Ser Gln Gly Gln Trp Thr Glu
Glu Val Pro Ser Cys Gln Val Val 480 485 490 aaa tgt tca agc ctg gca
gtt ccg gga aag atc aac atg agc tgc agt 1661 Lys Cys Ser Ser Leu
Ala Val Pro Gly Lys Ile Asn Met Ser Cys Ser 495 500 505 ggg gag ccc
gtg ttt ggc act gtg tgc aag ttc gcc tgt cct gaa gga 1709 Gly Glu
Pro Val Phe Gly Thr Val Cys Lys Phe Ala Cys Pro Glu Gly 510 515 520
tgg acg ctc aat ggc tct gca gct cgg aca tgt gga gcc aca gga cac
1757 Trp Thr Leu Asn Gly Ser Ala Ala Arg Thr Cys Gly Ala Thr Gly
His 525 530 535 tgg tct ggc ctg cta cct acc tgt gaa gct ccc act gag
tcc aac att 1805 Trp Ser Gly Leu Leu Pro Thr Cys Glu Ala Pro Thr
Glu Ser Asn Ile 540 545 550 555 ccc ttg gta gct gga ctt tct gct gct
gga ctc tcc ctc ctg aca tta 1853 Pro Leu Val Ala Gly Leu Ser Ala
Ala Gly Leu Ser Leu Leu Thr Leu 560 565 570 gca cca ttt ctc ctc tgg
ctt cgg aaa tgc tta cgg aaa gca aag aaa 1901 Ala Pro Phe Leu Leu
Trp Leu Arg Lys Cys Leu Arg Lys Ala Lys Lys 575 580 585 ttt gtt cct
gcc agc agc tgc caa agc ctt gaa tca gat gga agc tac 1949 Phe Val
Pro Ala Ser Ser Cys Gln Ser Leu Glu Ser Asp Gly Ser Tyr 590 595 600
caa aag cct tct tac atc ctt taa gttcaaaaga atcagaaaca ggtgcatctg
2003 Gln Lys Pro Ser Tyr Ile Leu * 605 610 gggaactaga gggatacact
gaagttaaca gagacagata actctcctcg ggtctctggc 2063 ccttcttgcc
tactatgcca gatgccttta tggctgaaac cgcaacaccc atcaccactt 2123
caatagatca aagtccagca ggcaaggacg gccttcaact gaaaagactc agtgttccct
2183 ttcctactct caggatcaag aaagtgttgg ctaatgaagg gaaaggatat
tttcttccaa 2243 gcaaaggtga agagaccaag actctgaaat ctcagaattc
cttttctaac tctcccttgc 2303 tcgctgtaaa atcttggcac agaaacacaa
tattttgtgg ctttctttct tttgcccttc 2363 acagtgtttc gacagctgat
tacacagttg ctgtcataag aatgaataat aattatccag 2423 agtttagagg
aaaaaaatga ctaaaaatat tataacttaa aaaatgacag atgttgaatg 2483
cccacaggca aatgcatgga gggttgttaa tggtgcaaat cctactgaat gctctgtgcg
2543 agggttacta tgcacaattt aatcactttc atccctatgg gattcagtgc
ttcttaaaga 2603 gttcttaagg attgtgatat ttttacttgc attgaatata
ttataatctt ccatacttct 2663 tcattcaata caagtgtggt agggacttaa
aaaacttgta aatgctgtca actatgatat 2723 ggtaaaagtt acttattcta
gattaccccc tcattgttta ttaacaaatt atgttacatc 2783 tgttttaaat
ttatttcaaa aagggaaact attgtcccct agcaaggcat gatgttaacc 2843
agaataaagt tctgagtgtt tttactacag ttgttttttg aaaacatggt agaattggag
2903 agtaaaaact gaatggaagg tttgtatatt gtcagatatt ttttcagaaa
tatgtggttt 2963 ccacgatgaa aaacttccat gaggccaaac gttttgaact
aataaaagca taaatgcaaa 3023 cacacaaagg tataatttta tgaatgtctt
tgttggaaaa gaatacagaa agatggatgt 3083 gctttgcatt cctacaaaga
tgtttgtcag atatgatatg taaacataat tcttgtatat 3143 tatggaagat
tttaaattca caatagaaac tcaccatgta aaagagtcat ctggtagatt 3203
tttaacgaat gaagatgtct aatagttatt ccctatttgt tttcttctgt atgttagggt
3263 gctctggaag agaggaatgc ctgtgtgagc aagcatttat gtttatttat
aagcagattt 3323 aacaattcca aaggaatctc cagttttcag ttgatcactg
gcaatgaaaa attctcagtc 3383 agtaattgcc aaagctgctc tagccttgag
gagtgtgaga atcaaaactc tcctacactt 3443 ccattaactt agcatgtgtt
gaaaaaaaag tttcagagaa gttctggctg aacactggca 3503 acaacaaagc
caacagtcaa aacagagatg tgataaggat cagaacagca gaggttcttt 3563
taaaggggca gaaaaactct gggaaataag agagaacaac tactgtgatc aggctatgta
3623 tggaatacag tgttattttc tttgaaattg tttaagtgtt gtaaatattt
atgtaaactg 3683 cattagaaat tagctgtgtg aaataccagt gtggtttgtg
tttgagtttt attgagaatt 3743 ttaaattata acttaaaata ttttataatt
tttaaagtat atatttattt aagcttatgt 3803 cagacctatt tgacataaca
ctataaaggt tgacaataaa tgtgcttatg tttaaaaaaa 3863 2 610 PRT Homo
Sapien 2 Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu
Leu Ile 1 5 10 15 Lys Glu Ser Gly Ala Trp Ser Tyr Asn Thr Ser Thr
Glu Ala Met Thr 20 25 30 Tyr Asp Glu Ala Ser Ala Tyr Cys Gln Gln
Arg Tyr Thr His Leu Val 35 40 45 Ala Ile Gln Asn Lys Glu Glu Ile
Glu Tyr Leu Asn Ser Ile Leu Ser 50 55 60 Tyr Ser Pro Ser Tyr Tyr
Trp Ile Gly Ile Arg Lys Val Asn Asn Val 65 70 75 80 Trp Val Trp Val
Gly Thr Gln Lys Pro Leu Thr Glu Glu Ala Lys Asn 85 90 95 Trp Ala
Pro Gly Glu Pro Asn Asn Arg Gln Lys Asp Glu Asp Cys Val 100 105 110
Glu Ile Tyr Ile Lys Arg Glu Lys Asp Val Gly Met Trp Asn Asp Glu 115
120 125 Arg Cys Ser Lys Lys Lys Leu Ala Leu Cys Tyr Thr Ala Ala Cys
Thr 130 135 140 Asn Thr Ser Cys Ser Gly His Gly Glu Cys Val Glu Thr
Ile Asn Asn 145 150 155 160 Tyr Thr Cys Lys Cys Asp Pro Gly Phe Ser
Gly Leu Lys Cys Glu Gln 165 170 175 Ile Val Asn Cys Thr Ala Leu Glu
Ser Pro Glu His Gly Ser Leu Val 180 185 190 Cys Ser His Pro Leu Gly
Asn Phe Ser Tyr Asn Ser Ser Cys Ser Ile 195 200 205 Ser Cys Asp Arg
Gly Tyr Leu Pro Ser Ser Met Glu Thr Met Gln Cys 210 215 220 Met Ser
Ser Gly Glu Trp Ser Ala Pro Ile Pro Ala Cys Asn Val Val 225 230 235
240 Glu Cys Asp Ala Val Thr Asn Pro Ala Asn Gly Phe Val Glu Cys Phe
245 250 255 Gln Asn Pro Gly Ser Phe Pro Trp Asn Thr Thr Cys Thr Phe
Asp Cys 260 265 270 Glu Glu Gly Phe Glu Leu Met Gly Ala Gln Ser Leu
Gln Cys Thr Ser 275 280 285 Ser Gly Asn Trp Asp Asn Glu Lys Pro Thr
Cys Lys Ala Val Thr Cys 290 295 300 Arg Ala Val Arg Gln Pro Gln Asn
Gly Ser Val Arg Cys Ser His Ser 305 310 315 320 Pro Ala Gly Glu Phe
Thr Phe Lys Ser Ser Cys Asn Phe Thr Cys Glu 325 330 335 Glu Gly Phe
Met Leu Gln Gly Pro Ala Gln Val Glu Cys Thr Thr Gln 340 345 350 Gly
Gln Trp Thr Gln Gln Ile Pro Val Cys Glu Ala Phe Gln Cys Thr 355 360
365 Ala Leu Ser Asn Pro Glu Arg Gly Tyr Met Asn Cys Leu Pro Ser Ala
370 375 380 Ser Gly Ser Phe Arg Tyr Gly Ser Ser Cys Glu Phe Ser Cys
Glu Gln 385 390 395 400 Gly Phe Val Leu Lys Gly Ser Lys Arg Leu Gln
Cys Gly Pro Thr Gly 405 410 415 Glu Trp Asp Asn Glu Lys Pro Thr Cys
Glu Ala Val Arg Cys Asp Ala 420 425 430 Val His Gln Pro Pro Lys Gly
Leu Val Arg Cys Ala His Ser Pro Ile 435 440 445 Gly Glu Phe Thr Tyr
Lys Ser Ser Cys Ala Phe Ser Cys Glu Glu Gly 450 455 460 Phe Glu Leu
His Gly Ser Thr Gln Leu Glu Cys Thr Ser Gln Gly Gln 465 470 475 480
Trp Thr Glu Glu Val Pro Ser Cys Gln Val Val Lys Cys Ser Ser Leu 485
490 495 Ala Val Pro Gly Lys Ile Asn Met Ser Cys Ser Gly Glu Pro Val
Phe 500 505 510 Gly Thr Val Cys Lys Phe Ala Cys Pro Glu Gly Trp Thr
Leu Asn Gly 515 520 525 Ser Ala Ala Arg Thr Cys Gly Ala Thr Gly His
Trp Ser Gly Leu Leu 530 535 540 Pro Thr Cys Glu Ala Pro Thr Glu Ser
Asn Ile Pro Leu Val Ala Gly 545 550 555 560 Leu Ser Ala Ala Gly Leu
Ser Leu Leu Thr Leu Ala Pro Phe Leu Leu 565 570 575 Trp Leu Arg Lys
Cys Leu Arg Lys Ala Lys Lys Phe Val Pro Ala Ser 580 585 590 Ser Cys
Gln Ser Leu Glu Ser Asp Gly Ser Tyr Gln Lys Pro Ser Tyr 595 600 605
Ile Leu 610 3 110 DNA Artificial Sequence Polylinkes of pNN11 3
gctagcggcc tccgcggcca gtccaaccac caatctcaaa gcataggcga catgcggccg
60 caaaacgatc agcagatcct cacatcccaa tccgaggccg cggtggccgc 110 4
2811 DNA Homo Sapien 4 cgggcctcac tggcttcagg agctgaatac cctcccaggc
acacacaggt gggacacaaa 60 taagggtttt ggaaccacta ttttctcatc
acgacagcaa cttaaaatgc ctgggaagat 120 ggtcgtgatc cttggagcct
caaatatact ttggataatg tttgcagctt ctcaagcttt 180 taaaatcgag
accaccccag aatctagata tcttgctcag attggtgact ccgtctcatt 240
gacttgcagc accacaggct gtgagtcccc atttttctct tggagaaccc agatagatag
300 tccactgaat gggaaggtga cgaatgaggg gaccacatct acgctgacaa
tgaatcctgt 360 tagttttggg aacgaacact cttacctgtg cacagcaact
tgtgaatcta ggaaattgga 420 aaaaggaatc caggtggaga tctactcttt
tcctaaggat ccagagattc atttgagtgg 480 ccctctggag gctgggaagc
cgatcacagt caagtgttca gttgctgatg tatacccatt 540 tgacaggctg
gagatagact tactgaaagg agatcatctc atgaagagtc aggaatttct 600
ggaggatgca gacaggaagt ccctggaaac caagagtttg gaagtaacct ttactcctgt
660 cattgaggat attggaaaag ttcttgtttg ccgagctaaa ttacacattg
atgaaatgga 720 ttctgtgccc acagtaaggc aggctgtaaa agaattgcaa
gtctacatat cacccaagaa 780 tacagttatt tctgtgaatc catccacaaa
gctgcaagaa ggtggctctg tgaccatgac 840 ctgttccagc gagggtctac
cagctccaga gattttctgg agtaagaaat tagataatgg 900 gaatctacag
cacctttctg gaaatgcaac tctcacctta attgctatga ggatggaaga 960
ttctggaatt tatgtgtgtg aaggagttaa tttgattggg aaaaacagaa aagaggtgga
1020 attaattgtt caagcattcc ctagagatcc agaaatcgag atgagtggtg
gcctcgtgaa 1080 tgggagctct gtcactgtaa gctgcaaggt tcctagcgtg
tacccccttg accggctgga 1140 gattgaatta cttaaggggg agactattct
ggagaatata gagtttttgg aggatacgga 1200 tatgaaatct ctagagaaca
aaagtttgga aatgaccttc atccctacca ttgaagatac 1260 tggaaaagct
cttgtttgtc aggctaagtt acatattgat gacatggaat tcgaacccaa 1320
acaaaggcag agtacgcaaa cactttatgt caatgttgcc cccagagata caaccgtctt
1380 ggtcagccct tcctccatcc tggaggaagg cagttctgtg aatatgacat
gcttgagcca 1440 gggctttcct gctccgaaaa tcctgtggag caggcagctc
cctaacgggg agctacagcc 1500 tctttctgag aatgcaactc tcaccttaat
ttctacaaaa atggaagatt ctggggttta 1560 tttatgtgaa ggaattaacc
aggctggaag aagcagaaag gaagtggaat taattatcca 1620 agttactcca
aaagacataa aacttacagc ttttccttct gagagtgtca aagaaggaga 1680
cactgtcatc atctcttgta catgtggaaa tgttccagaa acatggataa tcctgaagaa
1740 aaaagcggag acaggagaca cagtactaaa atctatagat ggcgcctata
ccatccgaaa 1800 ggcccagttg aaggatgcgg gagtatatga atgtgaatct
aaaaacaaag ttggctcaca 1860 attaagaagt ttaacacttg atgttcaagg
aagagaaaac aacaaagact atttttctcc 1920 tgagcttctc gtgctctatt
ttgcatcctc cttaataata cctgccattg gaatgataat 1980 ttactttgca
agaaaagcca acatgaaggg gtcatatagt cttgtagaag cacagaaatc 2040
aaaagtgtag ctaatgcttg atatgttcaa ctggagacac tatttatctg tgcaaatcct
2100 tgatactgct catcattcct tgagaaaaac aatgagctga gaggcagact
tccctgaatg 2160 tattgaactt ggaaagaaat gcccatctat gtcccttgct
gtgagcaaga agtcaaagta 2220 aaacttgctg cctgaagaac agtaactgcc
atcaagatga gagaactgga ggagttcctt 2280 gatctgtata tacaataaca
taatttgtac atatgtaaaa taaaattatg ccatagcaag 2340 attgcttaaa
atagcaacac tctatattta gattgttaaa ataactagtg ttgcttggac 2400
tattataatt taatgcatgt taggaaaatt tcacattaat atttgctgac agctgacctt
2460 tgtcatcttt cttctatttt attccctttc acaaaatttt attcctatat
agtttattga 2520 caataatttc aggttttgta aagatgccgg gttttatatt
tttatagaca aataataagc 2580 aaagggagca ctgggttgac tttcaggtac
taaatacctc aacctatggt ataatggttg 2640 actgggtttc tctgtatagt
actggcatgg tacggagatg tttcacgaag tttgttcatc 2700 agactcctgt
gcaactttcc caatgtggcc taaaaatgca acttcttttt attttctttt 2760
gtaaatgttt aggttttttt gtatagtaaa gtgataattt ctggaattaa a 2811 5
3080 DNA Homo Sapien 5 cactggcttc aggagctgaa taccctccca ggcacacaca
ggtgggacac aaataagggt 60 tttggaacca ctattttctc atcacgacag
caacttaaaa tgcctgggaa gatggtcgtg 120 atccttggag cctcaaatat
actttggata atgtttgcag cttctcaagc ttttaaaatc 180 gagaccaccc
cagaatctag atatcttgct cagattggtg actccgtctc attgacttgc 240
agcaccacag gctgtgagtc cccatttttc tcttggagaa cccagataga tagtccactg
300 aatgggaagg tgacgaatga ggggaccaca tctacgctga caatgaatcc
tgttagtttt 360 gggaacgaac actcttacct gtgcacagca acttgtgaat
ctaggaaatt ggaaaaagga 420 atccaggtgg agatctactc ttttcctaag
gatccagaga ttcatttgag tggccctctg 480 gaggctggga agccgatcac
agtcaagtgt tcagttgctg atgtataccc atttgacagg 540 ctggagatag
acttactgaa aggagatcat ctcatgaaga gtcaggaatt tctggaggat 600
gcagacagga agtccctgga aaccaagagt ttggaagtaa cctttactcc tgtcattgag
660 gatattggaa aagttcttgt ttgccgagct aaattacaca ttgatgaaat
ggattctgtg 720 cccacagtaa ggcaggctgt aaaagaattg caagtctaca
tatcacccaa gaatacagtt 780 atttctgtga atccatccac aaagctgcaa
gaaggtggct ctgtgaccat gacctgttcc 840 agcgagggtc taccagctcc
agagattttc tggagtaaga aattagataa tgggaatcta 900 cagcaccttt
ctggaaatgc aactctcacc ttaattgcta tgaggatgga agattctgga 960
atttatgtgt gtgaaggagt taatttgatt gggaaaaaca gaaaagaggt ggaattaatt
1020 gttcaagaga aaccatttac tgttgagatc tcccctggac cccggattgc
tgctcagatt 1080 ggagactcag tcatgttgac atgtagtgtc atgggctgtg
aatccccatc tttctcctgg 1140 agaacccaga tagacagccc tctgagcggg
aaggtgagga gtgaggggac caattccacg 1200 ctgaccctga gccctgtgag
ttttgagaac gaacactctt atctgtgcac agtgacttgt 1260 ggacataaga
aactggaaaa gggaatccag gtggagctct actcattccc tagagatcca 1320
gaaatcgaga tgagtggtgg cctcgtgaat gggagctctg tcactgtaag ctgcaaggtt
1380 cctagcgtgt acccccttga ccggctggag attgaattac ttaaggggga
gactattctg 1440 gagaatatag agtttttgga ggatacggat atgaaatctc
tagagaacaa aagtttggaa 1500 atgaccttca tccctaccat tgaagatact
ggaaaagctc ttgtttgtca ggctaagtta 1560 catattgatg acatggaatt
cgaacccaaa caaaggcaga gtacgcaaac actttatgtc 1620 aatgttgccc
ccagagatac aaccgtcttg gtcagccctt cctccatcct ggaggaaggc 1680
agttctgtga atatgacatg cttgagccag ggctttcctg ctccgaaaat cctgtggagc
1740 aggcagctcc ctaacgggga gctacagcct ctttctgaga atgcaactct
caccttaatt 1800 tctacaaaaa tggaagattc tggggtttat ttatgtgaag
gaattaacca ggctggaaga 1860 agcagaaagg aagtggaatt aattatccaa
gttactccaa aagacataaa acttacagct 1920 tttccttctg agagtgtcaa
agaaggagac actgtcatca tctcttgtac atgtggaaat 1980 gttccagaaa
catggataat cctgaagaaa aaagcggaga caggagacac agtactaaaa 2040
tctatagatg gcgcctatac catccgaaag gcccagttga aggatgcggg agtatatgaa
2100 tgtgaatcta aaaacaaagt tggctcacaa ttaagaagtt taacacttga
tgttcaagga 2160 agagaaaaca acaaagacta tttttctcct gagcttctcg
tgctctattt tgcatcctcc 2220 ttaataatac ctgccattgg aatgataatt
tactttgcaa gaaaagccaa catgaagggg 2280 tcatatagtc ttgtagaagc
acagaaatca aaagtgtagc taatgcttga tatgttcaac 2340 tggagacact
atttatctgt gcaaatcctt gatactgctc atcattcctt gagaaaaaca 2400
atgagctgag aggcagactt ccctgaatgt attgaacttg gaaagaaatg cccatctatg
2460 tcccttgctg tgagcaagaa gtcaaagtaa aacttgctgc ctgaagaaca
gtaactgcca 2520 tcaagatgag agaactggag gagttccttg atctgtatat
acaataacat aatttgtaca 2580 tatgtaaaat aaaattatgc catagcaaga
ttgcttaaaa tagcaacact ctatatttag 2640 attgttaaaa taactagtgt
tgcttggact attataattt aatgcatgtt aggaaaattt 2700 cacattaata
tttgctgaca gctgaccttt gtcatctttc ttctatttta ttccctttca 2760
caaaatttta ttcctatata gtttattgac aataatttca ggttttgtaa agatgccggg
2820 ttttatattt ttatagacaa ataataagca aagggagcac tgggttgact
ttcaggtact 2880 aaatacctca acctatggta taatggttga ctgggtttct
ctgtatagta ctggcatggt 2940 acggagatgt ttcacgaagt ttgttcatca
gactcctgtg caactttccc aatgtggcct 3000 aaaaatgcaa cttcttttta
ttttcttttg taaatgttta ggtttttttg tatagtaaag 3060 tgataatttc
tggaattaaa 3080 6 647 PRT Homo Sapien 6 Met Pro Gly Lys Met Val Val
Ile Leu Gly Ala Ser Asn Ile Leu Trp 1 5 10 15 Ile Met Phe Ala Ala
Ser Gln Ala Phe Lys Ile Glu Thr Thr Pro Glu 20 25 30 Ser Arg Tyr
Leu Ala Gln Ile Gly Asp Ser Val Ser Leu Thr Cys Ser 35 40 45 Thr
Thr Gly Cys Glu Ser Pro Phe Phe Ser Trp Arg Thr Gln Ile Asp 50 55
60 Ser Pro Leu Asn Gly Lys Val Thr Asn Glu Gly Thr Thr Ser Thr Leu
65 70 75 80 Thr Met Asn Pro Val Ser Phe Gly Asn Glu His Ser Tyr Leu
Cys Thr 85 90 95 Ala Thr Cys Glu Ser Arg Lys Leu Glu Lys Gly Ile
Gln Val Glu Ile 100 105 110 Tyr Ser Phe Pro Lys Asp Pro Glu Ile His
Leu Ser Gly Pro Leu Glu 115 120 125 Ala Gly Lys Pro Ile Thr Val Lys
Cys Ser Val Ala Asp Val Tyr Pro 130 135 140 Phe Asp Arg Leu Glu Ile
Asp Leu Leu Lys Gly Asp His Leu Met Lys 145 150 155 160 Ser Gln Glu
Phe Leu Glu Asp Ala Asp Arg Lys Ser Leu Glu Thr Lys 165 170 175 Ser
Leu Glu Val Thr Phe Thr Pro Val Ile Glu Asp Ile Gly Lys Val 180 185
190 Leu Val Cys Arg Ala Lys Leu His Ile Asp Glu Met Asp Ser Val Pro
195 200 205 Thr Val Arg Gln Ala Val Lys Glu Leu Gln Val Tyr Ile Ser
Pro Lys 210 215 220 Asn Thr Val Ile Ser Val Asn Pro Ser Thr Lys Leu
Gln Glu Gly Gly 225 230 235 240 Ser Val Thr Met Thr Cys Ser Ser Glu
Gly Leu Pro Ala Pro Glu Ile 245 250 255 Phe Trp Ser Lys Lys Leu Asp
Asn Gly Asn Leu Gln His Leu Ser Gly 260 265 270 Asn Ala Thr Leu Thr
Leu Ile Ala Met Arg Met Glu Asp Ser Gly Ile 275 280 285 Tyr Val Cys
Glu Gly Val Asn Leu Ile Gly Lys Asn Arg Lys Glu Val 290 295 300 Glu
Leu Ile Val Gln Ala Phe Pro Arg Asp Pro Glu Ile Glu Met Ser 305 310
315 320 Gly Gly Leu Val Asn Gly Ser Ser Val Thr Val Ser Cys Lys Val
Pro 325 330 335 Ser Val Tyr Pro Leu Asp Arg Leu Glu Ile Glu Leu Leu
Lys Gly Glu 340 345 350 Thr Ile Leu Glu Asn Ile Glu Phe Leu Glu Asp
Thr Asp Met Lys Ser 355 360 365 Leu Glu Asn Lys Ser Leu Glu Met Thr
Phe Ile Pro Thr Ile Glu Asp 370 375 380 Thr Gly Lys Ala Leu Val Cys
Gln Ala Lys Leu His Ile Asp Asp Met 385 390 395 400 Glu Phe Glu Pro
Lys Gln Arg Gln Ser Thr Gln Thr Leu Tyr Val Asn 405 410 415 Val Ala
Pro Arg Asp Thr Thr Val Leu Val Ser Pro Ser Ser Ile Leu 420 425 430
Glu Glu Gly Ser Ser Val Asn Met Thr Cys Leu Ser Gln Gly Phe Pro 435
440 445 Ala Pro Lys Ile Leu Trp Ser Arg Gln Leu Pro Asn Gly Glu Leu
Gln 450 455 460 Pro Leu Ser Glu Asn Ala Thr Leu Thr Leu Ile Ser Thr
Lys Met Glu 465 470 475 480 Asp Ser Gly Val Tyr Leu Cys Glu Gly Ile
Asn Gln Ala Gly Arg Ser 485 490 495 Arg Lys Glu Val Glu Leu Ile Ile
Gln Val Thr Pro Lys Asp Ile Lys 500 505 510 Leu Thr Ala Phe Pro Ser
Glu Ser Val Lys Glu Gly Asp Thr Val Ile 515 520 525 Ile Ser Cys Thr
Cys Gly Asn Val Pro Glu Thr Trp Ile Ile Leu Lys 530 535 540 Lys Lys
Ala Glu Thr Gly Asp Thr Val Leu Lys Ser Ile Asp Gly Ala 545 550 555
560 Tyr Thr Ile Arg Lys Ala Gln Leu Lys Asp Ala Gly Val Tyr Glu Cys
565 570 575 Glu Ser Lys Asn Lys Val Gly Ser Gln Leu Arg Ser Leu Thr
Leu Asp 580 585 590 Val Gln Gly Arg Glu Asn Asn Lys Asp Tyr Phe Ser
Pro Glu Leu Leu 595 600 605 Val Leu Tyr Phe Ala Ser Ser Leu Ile Ile
Pro Ala Ile Gly Met Ile 610 615 620 Ile Tyr Phe Ala Arg Lys Ala Asn
Met Lys Gly Ser Tyr Ser Leu Val 625 630 635 640 Glu Ala Gln Lys Ser
Lys Val 645 7 739 PRT Homo Sapien 7 Met Pro Gly Lys Met Val Val Ile
Leu Gly Ala Ser Asn Ile Leu Trp 1 5 10 15 Ile Met Phe Ala Ala Ser
Gln Ala Phe Lys Ile Glu Thr Thr Pro Glu 20 25 30 Ser Arg Tyr Leu
Ala Gln Ile Gly Asp Ser Val Ser Leu Thr Cys Ser 35 40 45 Thr Thr
Gly Cys Glu Ser Pro Phe Phe Ser Trp Arg Thr Gln Ile Asp 50 55 60
Ser Pro Leu Asn Gly Lys Val Thr Asn Glu Gly Thr Thr Ser Thr Leu 65
70 75 80 Thr Met Asn Pro Val Ser Phe Gly Asn Glu His Ser Tyr Leu
Cys Thr 85 90 95 Ala Thr Cys Glu Ser Arg Lys Leu Glu Lys Gly Ile
Gln Val Glu Ile 100 105 110 Tyr Ser Phe Pro Lys Asp Pro Glu Ile His
Leu Ser Gly Pro Leu Glu 115 120 125 Ala Gly Lys Pro Ile Thr Val Lys
Cys Ser Val Ala Asp Val Tyr Pro 130 135 140 Phe Asp Arg Leu Glu Ile
Asp Leu Leu Lys Gly Asp His Leu Met Lys 145 150 155 160 Ser Gln Glu
Phe Leu Glu Asp Ala Asp Arg Lys Ser Leu Glu Thr Lys 165 170 175 Ser
Leu Glu Val Thr Phe Thr Pro Val Ile Glu Asp Ile Gly Lys Val 180 185
190 Leu Val Cys Arg Ala Lys Leu His Ile Asp Glu Met Asp Ser Val Pro
195 200 205 Thr Val Arg Gln Ala Val Lys Glu Leu Gln Val Tyr Ile Ser
Pro Lys 210 215 220 Asn Thr Val Ile Ser Val Asn Pro Ser Thr Lys Leu
Gln Glu Gly Gly 225 230 235 240 Ser Val Thr Met Thr Cys Ser Ser Glu
Gly Leu Pro Ala Pro Glu Ile 245 250 255 Phe Trp Ser Lys Lys Leu Asp
Asn Gly Asn Leu Gln His Leu Ser Gly 260 265 270 Asn Ala Thr Leu Thr
Leu Ile Ala Met Arg Met Glu Asp Ser Gly Ile 275 280 285 Tyr Val Cys
Glu Gly Val Asn Leu Ile Gly Lys Asn Arg Lys Glu Val 290 295 300 Glu
Leu Ile Val Gln Glu Lys Pro Phe Thr Val Glu Ile Ser Pro Gly 305 310
315 320 Pro Arg Ile Ala Ala Gln Ile Gly Asp Ser Val Met Leu Thr Cys
Ser 325 330 335 Val Met Gly Cys Glu Ser Pro Ser Phe Ser Trp Arg Thr
Gln Ile Asp 340 345 350 Ser Pro Leu Ser Gly Lys Val Arg Ser Glu Gly
Thr Asn Ser Thr Leu 355 360 365 Thr Leu Ser Pro Val Ser Phe Glu Asn
Glu His Ser Tyr Leu Cys Thr 370 375 380 Val Thr Cys Gly His Lys Lys
Leu Glu Lys Gly Ile Gln Val Glu Leu 385 390 395 400 Tyr Ser Phe Pro
Arg Asp Pro Glu Ile Glu Met Ser Gly Gly Leu Val 405 410 415 Asn Gly
Ser Ser Val Thr Val Ser Cys Lys Val Pro Ser Val Tyr Pro 420 425 430
Leu Asp Arg Leu Glu Ile Glu Leu Leu Lys Gly Glu Thr Ile Leu Glu 435
440 445 Asn Ile Glu Phe Leu Glu Asp Thr Asp Met Lys Ser Leu Glu Asn
Lys 450 455 460 Ser Leu Glu Met Thr Phe Ile Pro Thr Ile Glu Asp Thr
Gly Lys Ala 465 470 475 480 Leu Val Cys Gln Ala Lys Leu His Ile Asp
Asp Met Glu Phe Glu Pro 485 490 495 Lys Gln Arg Gln Ser Thr Gln Thr
Leu Tyr Val Asn Val Ala Pro Arg 500 505 510 Asp Thr Thr Val Leu Val
Ser Pro Ser Ser Ile Leu Glu Glu Gly Ser 515 520 525 Ser Val Asn Met
Thr Cys Leu Ser Gln Gly Phe Pro Ala Pro Lys Ile 530 535 540 Leu Trp
Ser Arg Gln Leu Pro Asn Gly Glu Leu Gln Pro Leu Ser Glu 545 550 555
560 Asn Ala Thr Leu Thr Leu Ile Ser Thr Lys Met Glu Asp Ser Gly Val
565 570 575 Tyr Leu Cys Glu Gly Ile Asn Gln Ala Gly Arg Ser Arg Lys
Glu Val 580 585 590 Glu Leu Ile Ile Gln Val Thr Pro Lys Asp Ile Lys
Leu Thr Ala Phe 595 600 605 Pro Ser Glu Ser Val Lys Glu Gly Asp Thr
Val Ile Ile Ser Cys Thr 610 615 620 Cys Gly Asn Val Pro Glu Thr Trp
Ile Ile Leu Lys Lys Lys Ala Glu 625 630 635 640 Thr Gly Asp Thr Val
Leu Lys Ser Ile Asp Gly Ala Tyr Thr Ile Arg 645 650 655 Lys Ala Gln
Leu Lys Asp Ala Gly Val Tyr Glu Cys Glu Ser Lys Asn 660 665 670 Lys
Val Gly Ser Gln Leu Arg Ser Leu Thr Leu Asp Val Gln Gly Arg 675 680
685 Glu Asn Asn Lys Asp Tyr Phe Ser Pro Glu Leu Leu Val Leu Tyr Phe
690 695 700 Ala Ser Ser Leu Ile Ile Pro Ala Ile Gly Met Ile Ile Tyr
Phe Ala 705 710 715 720 Arg Lys Ala Asn Met Lys Gly Ser Tyr Ser Leu
Val Glu Ala Gln Lys 725 730 735 Ser Lys Val 8 1566 DNA Homo Sapien
8 gcatgcgcca ccatgcccag ctaattttgt atttttagta gagatggcgt ttctccatgt
60 tggtcaggct ggtcttgaac tcccggcctc aggtgatccg cctgcctcgg
cctcccaaag 120 tggtgggatt acaggcgtga gccactgtgc ctggcctcct
ttttattttt ttcactgaac 180 aaaccatgaa actttcccag atgtaaatat
ctatttccca tttttctttt tttaaaataa 240 ggcattattt taaccatttg
agtgttagat attattttta gataatattt taatttaggc 300 ataactgccg
tgcaaaatct gaagattaat atctaccttg tgagtcattc ctctgtgaga 360
cagtgcatgt taaatatgtt gaattggcag gtgaaaaagg aagaaaaaat gagtagtgat
420 tggttatcca cagctatgaa tgagaaattg aaggtagtag actatggatg
acaaacctat 480 tcttggtttc cttctgtttc tgaaattcta attactacca
caactacatg agagacacta 540 ctaacaagca aagttttaca actttttaaa
gacatagact ttatgttatt ataattaaaa 600 atcatgcatt tttgtcatat
taataaaatt gcatatacga tataaaggca tggacaaagg 660 tgaagtagct
tcaagagaca gagtttctga catcattgta attttaagca tcgtggatat 720
tcccgggaaa gtttttggat gccattgggg atttcctctt tactggatgt ggacaatatc
780 ctcctattat tcacaggaag caatccctcc tataaaaggg cctcagccga
agtagtgttc 840 agctgttctt ggctgacttc acatcaaaac tcctatactg
acctgagaca gaggcagcag 900 tgatacccac ctgagagatc ctgtgtttga
acaactgctt cccaaaacgg taagtgcaga 960 acgctttata agggcagcct
cgggccatga aacacagata tgcaaaaggc cttctaataa 1020 aaaccacatc
tgtacaagct cttattgtat tgtagctaaa acctgtcttt tctctttgac 1080
ctaaataatg aaagtcttaa aatttgttta tttatttgat taaactctga aataaagatt
1140 attgcactag tgtcctttgc ccaaaatctt aggatgctgc cttaaacatc
atggtagaat 1200 aatgtaacta gctacccacg atttccttct ttaattcatt
ttgtgtttta tctccccagg 1260 aaagtatttc aagcctaaac ctttgggtga
aaagaactct tgaagtcatg attgcttcac 1320 agtttctctc agctctcact
ttgggtaagt cagtgccatt agaccaagat ttctcattct 1380 cgcactatag
atatttcaga ctgaaatatc cttgcttgtc tggggctgtc ctgcacagga 1440
tatctggcag catccttgac ctctacctgc aatgtgttct tccctgggct tggggtcatt
1500 tactttacct cttggtgtct ccctttcctt aagtgtaaag tgtggatccg
ttgacctgca 1560 ggtcga 1566 9 1032 DNA Homo Sapien 9 aagcttcatt
ctgcaatcag cattgtcctt tatctttcca gtaaagatag ccttttggag 60
tcgaagatga ggaaaagcct gtattttata gtcttggaag tgtcttcttt tgccaggaca
120 gagagaggag cttcagcagt gagagcaact gaaggggtta atagtggaac
ttggctgggt 180 gtctgttaaa cttttttccc tggctctgcc ctgggtttcc
ccttgaaggg atttccctcc 240 gcctctgcaa caagaccctt tataaagcac
agactttcta tttcactccg cggtatctgc 300 atcgggcctc actggcttca
ggagctgaat accctcccag gcacacacag gtgggacaca 360 aataagggtt
ttggaaccac tattttctca tcacgacagc aacttaaaat gcctgggaag 420
atggtcgtga tccttggagc ctcaaatata ctttggataa tgtttgcagc ttgtaagttc
480 tttcccttca tctgtttcaa atgttagcat tcaattttag ccctggtttt
ggcttcagtc 540 agttttgcga tagtagtgaa gtaaagacac taggatttta
aacagtagga aaagttaatt 600 tagtctaact tttaatatgc aattgagttt
tgctatatac cattgtactg tcatagttag 660 agctgaaaat tgatgttttt
ggtatctttt tttccaaagg caattgagta atttggattc 720 tgtctctagt
cggtctgtct ctttagtttc ctatacttga caatgaggtc aaacttagca 780
aataacaaaa cagctttgat aaatgggcat caaggttgga actgagaatt ctctctactg
840 aagatgattc cataaacttt tttggcagtg acttcggtgc tttttggcta
ccttgcagtt 900 agtttgaagc agcttaggaa agagatcagt ctgatcatat
tttagacatt atacagagaa 960 gaaggaggag aatactagac aaactagtgg
taaatttgct tctgtctttt tttgcttttg 1020 cagctcaagc tt 1032 10 2175
DNA Homo Sapien CDS (66)...(1283) 10 gcggccgccg ctcctccacg
cctgcggacg cgtggcgagc ggaggcagcg ctgcctgttc 60 gcgcc atg ggg gca
ccg tgg ggc tcg ccg acg gcg gcg gcg ggc ggg cgg 110 Met Gly Ala Pro
Trp Gly Ser Pro Thr Ala Ala Ala Gly Gly Arg 1 5 10 15 cgc ggg tgg
cgc cga ggc cgg ggg ctg cca tgg acc gtc tgt gtg ctg 158 Arg Gly Trp
Arg Arg Gly Arg Gly Leu Pro Trp Thr Val Cys Val Leu 20 25 30 gcg
gcc gcc ggc ttg acg tgt acg gcg ctg atc acc tac gct tgc tgg 206 Ala
Ala Ala Gly Leu Thr Cys Thr Ala Leu Ile Thr Tyr Ala Cys Trp 35 40
45 ggg cag ctg ccg ccg ctg ccc tgg gcg tcg cca acc ccg tcg cga ccg
254 Gly Gln Leu Pro Pro Leu Pro Trp Ala Ser Pro Thr Pro Ser Arg Pro
50 55
60 gtg ggc gtg ctg ctg tgg tgg gag ccc ttc ggg ggg cgc gat agc gcc
302 Val Gly Val Leu Leu Trp Trp Glu Pro Phe Gly Gly Arg Asp Ser Ala
65 70 75 ccg agg ccg ccc cct gac tgc cgg ctg cgc ttc aac atc agc
ggc tgc 350 Pro Arg Pro Pro Pro Asp Cys Arg Leu Arg Phe Asn Ile Ser
Gly Cys 80 85 90 95 cgc ctg ctc acc gac cgc gcg tcc tac gga gag gct
cag gcc gtg ctt 398 Arg Leu Leu Thr Asp Arg Ala Ser Tyr Gly Glu Ala
Gln Ala Val Leu 100 105 110 ttc cac cac cgc gac ctc gtg aag ggg ccc
ccc gac tgg ccc ccg ccc 446 Phe His His Arg Asp Leu Val Lys Gly Pro
Pro Asp Trp Pro Pro Pro 115 120 125 tgg ggc atc cag gcg cac act gcc
gag gag gtg gat ctg cgc gtg ttg 494 Trp Gly Ile Gln Ala His Thr Ala
Glu Glu Val Asp Leu Arg Val Leu 130 135 140 gac tac gag gag gca gcg
gcg gcg gca gaa gcc ctg gcg acc tcc agc 542 Asp Tyr Glu Glu Ala Ala
Ala Ala Ala Glu Ala Leu Ala Thr Ser Ser 145 150 155 ccc agg ccc ccg
ggc cag cgc tgg gtt tgg atg aac ttc gag tcg ccc 590 Pro Arg Pro Pro
Gly Gln Arg Trp Val Trp Met Asn Phe Glu Ser Pro 160 165 170 175 tcg
cac tcc ccg ggg ctg cga agc ctg gca agt aac ctc ttc aac tgg 638 Ser
His Ser Pro Gly Leu Arg Ser Leu Ala Ser Asn Leu Phe Asn Trp 180 185
190 acg ctc tcc tac cgg gcg gac tcg gac gtc ttt gtg cct tat ggc tac
686 Thr Leu Ser Tyr Arg Ala Asp Ser Asp Val Phe Val Pro Tyr Gly Tyr
195 200 205 ctc tac ccc aga agc cac ccc ggc gac ccg ccc tca ggc ctg
gcc ccg 734 Leu Tyr Pro Arg Ser His Pro Gly Asp Pro Pro Ser Gly Leu
Ala Pro 210 215 220 cca ctg tcc agg aaa cag ggg ctg gtg gca tgg gtg
gtg agc cac tgg 782 Pro Leu Ser Arg Lys Gln Gly Leu Val Ala Trp Val
Val Ser His Trp 225 230 235 gac gag cgc cag gcc cgg gtc cgc tac tac
cac caa ctg agc caa cat 830 Asp Glu Arg Gln Ala Arg Val Arg Tyr Tyr
His Gln Leu Ser Gln His 240 245 250 255 gtg acc gtg gac gtg ttc ggc
cgg ggc ggg ccg ggg cag ccg gtg ccc 878 Val Thr Val Asp Val Phe Gly
Arg Gly Gly Pro Gly Gln Pro Val Pro 260 265 270 gaa att ggg ctc ctg
cac aca gtg gcc cgc tac aag ttc tac ctg gct 926 Glu Ile Gly Leu Leu
His Thr Val Ala Arg Tyr Lys Phe Tyr Leu Ala 275 280 285 ttc gag aac
tcg cag cac ctg gat tat atc acc gag aag ctc tgg cgc 974 Phe Glu Asn
Ser Gln His Leu Asp Tyr Ile Thr Glu Lys Leu Trp Arg 290 295 300 aac
gcg ttg ctc gct ggg gcg gtg ccg gtg gtg ctg ggc cca gac cgt 1022
Asn Ala Leu Leu Ala Gly Ala Val Pro Val Val Leu Gly Pro Asp Arg 305
310 315 gcc aac tac gag cgc ttt gtg ccc cgc ggc gcc ttc atc cac gtg
gac 1070 Ala Asn Tyr Glu Arg Phe Val Pro Arg Gly Ala Phe Ile His
Val Asp 320 325 330 335 gac ttc cca agt gcc tcc tcc ctg gcc tcg tac
ctg ctt ttc ctc gac 1118 Asp Phe Pro Ser Ala Ser Ser Leu Ala Ser
Tyr Leu Leu Phe Leu Asp 340 345 350 cgc aac ccc gcg gtc tat cgc cgc
tac ttc cac tgg cgc cgg agc tac 1166 Arg Asn Pro Ala Val Tyr Arg
Arg Tyr Phe His Trp Arg Arg Ser Tyr 355 360 365 gct gtc cac atc acc
tcc ttc tgg gac gag cct tgg tgc cgg gtg tgc 1214 Ala Val His Ile
Thr Ser Phe Trp Asp Glu Pro Trp Cys Arg Val Cys 370 375 380 cag gct
gta cag agg gct ggg gac cgg ccc aag agc ata cgg aac ttg 1262 Gln
Ala Val Gln Arg Ala Gly Asp Arg Pro Lys Ser Ile Arg Asn Leu 385 390
395 gcc agc tgg ttc gag cgg tga agccgcgctc ccctggaagc gacccagggg
1313 Ala Ser Trp Phe Glu Arg * 400 405 aggccaagtt gtcagctttt
tgatcctcta ctgtgcatct ccttgactgc cgcatcatgg 1373 gagtaagttc
ttcaaacacc catttttgct ctatgggaaa aaaacgattt accaattaat 1433
attactcagc acagagatgg gggcccggtt tccatatttt ttgcacagct agcaattggg
1493 ctccctttgc tgctgatggg catcattgtt taggggtgaa ggagggggtt
cttcctcacc 1553 ttgtaaccag tgcagaaatg aaatagctta gcggcaagaa
gccgttgagg cggtttcctg 1613 aatttcccca tctgccacag gccatatttg
tggcccgtgc agcttccaaa tctcatacac 1673 aactgttccc gattcacgtt
tttctggacc aaggtgaagc aaatttgtgg ttgtagaagg 1733 agccttgttg
gtggagagtg gaaggactgt ggctgcaggt gggactttgt tgtttggatt 1793
cctcacagcc ttggctcctg agaaaggtga ggagggcagt ccaagagggg ccgctgactt
1853 ctttcacaag tactatctgt tcccctgtcc tgtgaatgga agcaaagtgc
tggattgtcc 1913 ttggaggaaa cttaagatga atacatgcgt gtacctcact
ttacataaga aatgtattcc 1973 tgaaaagctg catttaaatc aagtcccaaa
ttcattgact taggggagtt cagtatttaa 2033 tgaaacccta tggagaattt
atccctttac aatgtgaata gtcatctcct aatttgtttc 2093 ttctgtcttt
atgtttttct ataacctgga ttttttaaat catattaaaa ttacagatgt 2153
gaaaataaaa aaaagcggcc gc 2175 11 405 PRT Homo Sapien 11 Met Gly Ala
Pro Trp Gly Ser Pro Thr Ala Ala Ala Gly Gly Arg Arg 1 5 10 15 Gly
Trp Arg Arg Gly Arg Gly Leu Pro Trp Thr Val Cys Val Leu Ala 20 25
30 Ala Ala Gly Leu Thr Cys Thr Ala Leu Ile Thr Tyr Ala Cys Trp Gly
35 40 45 Gln Leu Pro Pro Leu Pro Trp Ala Ser Pro Thr Pro Ser Arg
Pro Val 50 55 60 Gly Val Leu Leu Trp Trp Glu Pro Phe Gly Gly Arg
Asp Ser Ala Pro 65 70 75 80 Arg Pro Pro Pro Asp Cys Arg Leu Arg Phe
Asn Ile Ser Gly Cys Arg 85 90 95 Leu Leu Thr Asp Arg Ala Ser Tyr
Gly Glu Ala Gln Ala Val Leu Phe 100 105 110 His His Arg Asp Leu Val
Lys Gly Pro Pro Asp Trp Pro Pro Pro Trp 115 120 125 Gly Ile Gln Ala
His Thr Ala Glu Glu Val Asp Leu Arg Val Leu Asp 130 135 140 Tyr Glu
Glu Ala Ala Ala Ala Ala Glu Ala Leu Ala Thr Ser Ser Pro 145 150 155
160 Arg Pro Pro Gly Gln Arg Trp Val Trp Met Asn Phe Glu Ser Pro Ser
165 170 175 His Ser Pro Gly Leu Arg Ser Leu Ala Ser Asn Leu Phe Asn
Trp Thr 180 185 190 Leu Ser Tyr Arg Ala Asp Ser Asp Val Phe Val Pro
Tyr Gly Tyr Leu 195 200 205 Tyr Pro Arg Ser His Pro Gly Asp Pro Pro
Ser Gly Leu Ala Pro Pro 210 215 220 Leu Ser Arg Lys Gln Gly Leu Val
Ala Trp Val Val Ser His Trp Asp 225 230 235 240 Glu Arg Gln Ala Arg
Val Arg Tyr Tyr His Gln Leu Ser Gln His Val 245 250 255 Thr Val Asp
Val Phe Gly Arg Gly Gly Pro Gly Gln Pro Val Pro Glu 260 265 270 Ile
Gly Leu Leu His Thr Val Ala Arg Tyr Lys Phe Tyr Leu Ala Phe 275 280
285 Glu Asn Ser Gln His Leu Asp Tyr Ile Thr Glu Lys Leu Trp Arg Asn
290 295 300 Ala Leu Leu Ala Gly Ala Val Pro Val Val Leu Gly Pro Asp
Arg Ala 305 310 315 320 Asn Tyr Glu Arg Phe Val Pro Arg Gly Ala Phe
Ile His Val Asp Asp 325 330 335 Phe Pro Ser Ala Ser Ser Leu Ala Ser
Tyr Leu Leu Phe Leu Asp Arg 340 345 350 Asn Pro Ala Val Tyr Arg Arg
Tyr Phe His Trp Arg Arg Ser Tyr Ala 355 360 365 Val His Ile Thr Ser
Phe Trp Asp Glu Pro Trp Cys Arg Val Cys Gln 370 375 380 Ala Val Gln
Arg Ala Gly Asp Arg Pro Lys Ser Ile Arg Asn Leu Ala 385 390 395 400
Ser Trp Phe Glu Arg 405 12 2861 DNA Homo Sapien CDS (174)...(1766)
12 ctgctcctgc gcggcagctg ctttagaagg tctcgagcct cctgtacctt
cccagggatg 60 aaccgggcct tccctctgga aggcgagggt tcgggccaca
gtgagcgagg gccagggcgg 120 tgggcgcgcg cagagggaaa ccggatcagt
tgagagagaa tcaagagtag cgg atg 176 Met 1 agg cgc ttg tgg ggc gcg gcc
cgg aag ccc tcg ggc gcg ggc tgg gag 224 Arg Arg Leu Trp Gly Ala Ala
Arg Lys Pro Ser Gly Ala Gly Trp Glu 5 10 15 aag gag tgg gcg gag gcg
ccg cag gag gct ccc ggg gcc tgg tcg ggc 272 Lys Glu Trp Ala Glu Ala
Pro Gln Glu Ala Pro Gly Ala Trp Ser Gly 20 25 30 cgg ctg ggc ccc
ggg cgc agt gga aga aag gga cgg gcg gtg ccc ggt 320 Arg Leu Gly Pro
Gly Arg Ser Gly Arg Lys Gly Arg Ala Val Pro Gly 35 40 45 tgg gcg
tcc tgg cca gct cac ctt gcc ctg gcg gct cgc ccc gcc cgg 368 Trp Ala
Ser Trp Pro Ala His Leu Ala Leu Ala Ala Arg Pro Ala Arg 50 55 60 65
cac ttg gga gga gca ggg cag ggc ccg cgg cct ttg cat tct ggg acc 416
His Leu Gly Gly Ala Gly Gln Gly Pro Arg Pro Leu His Ser Gly Thr 70
75 80 gcc ccc ttc cat tcc cgg gcc agc ggc gag cgg cag cga cgg ctg
gag 464 Ala Pro Phe His Ser Arg Ala Ser Gly Glu Arg Gln Arg Arg Leu
Glu 85 90 95 ccg cag cta cag cat gag agc cgg tgc cgc tcc tcc acg
cct gcg gac 512 Pro Gln Leu Gln His Glu Ser Arg Cys Arg Ser Ser Thr
Pro Ala Asp 100 105 110 gcg tgg cga gcg gag gca gcg ctg cct gtt cgc
gcc atg ggg gca ccg 560 Ala Trp Arg Ala Glu Ala Ala Leu Pro Val Arg
Ala Met Gly Ala Pro 115 120 125 tgg ggc tcg ccg acg gcg gcg gcg ggc
ggg cgg cgc ggg tgg cgc cga 608 Trp Gly Ser Pro Thr Ala Ala Ala Gly
Gly Arg Arg Gly Trp Arg Arg 130 135 140 145 ggc cgg ggg ctg cca tgg
acc gtc tgt gtg ctg gcg gcc gcc ggc ttg 656 Gly Arg Gly Leu Pro Trp
Thr Val Cys Val Leu Ala Ala Ala Gly Leu 150 155 160 acg tgt acg gcg
ctg atc acc tac gct tgc tgg ggg cag ctg ccg ccg 704 Thr Cys Thr Ala
Leu Ile Thr Tyr Ala Cys Trp Gly Gln Leu Pro Pro 165 170 175 ctg ccc
tgg gcg tcg cca acc ccg tcg cga ccg gtg ggc gtg ctg ctg 752 Leu Pro
Trp Ala Ser Pro Thr Pro Ser Arg Pro Val Gly Val Leu Leu 180 185 190
tgg tgg gag ccc ttc ggg ggg cgc gat agc gcc ccg agg ccg ccc cct 800
Trp Trp Glu Pro Phe Gly Gly Arg Asp Ser Ala Pro Arg Pro Pro Pro 195
200 205 gac tgc cgg ctg cgc ttc aac atc agc ggc tgc cgc ctg ctc acc
gac 848 Asp Cys Arg Leu Arg Phe Asn Ile Ser Gly Cys Arg Leu Leu Thr
Asp 210 215 220 225 cgc gcg tcc tac gga gag gct cag gcc gtg ctt ttc
cac cac cgc gac 896 Arg Ala Ser Tyr Gly Glu Ala Gln Ala Val Leu Phe
His His Arg Asp 230 235 240 ctc gtg aag ggg ccc ccc gac tgg ccc ccg
ccc tgg ggc atc cag gcg 944 Leu Val Lys Gly Pro Pro Asp Trp Pro Pro
Pro Trp Gly Ile Gln Ala 245 250 255 cac act gcc gag gag gtg gat ctg
cgc gtg ttg gac tac gag gag gca 992 His Thr Ala Glu Glu Val Asp Leu
Arg Val Leu Asp Tyr Glu Glu Ala 260 265 270 gcg gcg gcg gca gaa gcc
ctg gcg acc tcc agc ccc agg ccc ccg ggc 1040 Ala Ala Ala Ala Glu
Ala Leu Ala Thr Ser Ser Pro Arg Pro Pro Gly 275 280 285 cag cgc tgg
gtt tgg atg aac ttc gag tcg ccc tcg cac tcc ccg ggg 1088 Gln Arg
Trp Val Trp Met Asn Phe Glu Ser Pro Ser His Ser Pro Gly 290 295 300
305 ctg cga agc ctg gca agt aac ctc ttc aac tgg acg ctc tcc tac cgg
1136 Leu Arg Ser Leu Ala Ser Asn Leu Phe Asn Trp Thr Leu Ser Tyr
Arg 310 315 320 gcg gac tcg gac gtc ttt gtg cct tat ggc tac ctc tac
ccc aga agc 1184 Ala Asp Ser Asp Val Phe Val Pro Tyr Gly Tyr Leu
Tyr Pro Arg Ser 325 330 335 cac ccc ggc gac ccg ccc tca ggc ctg gcc
ccg cca ctg tcc agg aaa 1232 His Pro Gly Asp Pro Pro Ser Gly Leu
Ala Pro Pro Leu Ser Arg Lys 340 345 350 cag ggg ctg gtg gca tgg gtg
gtg agc cac tgg gac gag cgc cag gcc 1280 Gln Gly Leu Val Ala Trp
Val Val Ser His Trp Asp Glu Arg Gln Ala 355 360 365 cgg gtc cgc tac
tac cac caa ctg agc caa cat gtg acc gtg gac gtg 1328 Arg Val Arg
Tyr Tyr His Gln Leu Ser Gln His Val Thr Val Asp Val 370 375 380 385
ttc ggc cgg ggc ggg ccg ggg cag ccg gtg ccc gaa att ggg ctc ctg
1376 Phe Gly Arg Gly Gly Pro Gly Gln Pro Val Pro Glu Ile Gly Leu
Leu 390 395 400 cac aca gtg gcc cgc tac aag ttc tac ctg gct ttc gag
aac tcg cag 1424 His Thr Val Ala Arg Tyr Lys Phe Tyr Leu Ala Phe
Glu Asn Ser Gln 405 410 415 cac ctg gat tat atc acc gag aag ctc tgg
cgc aac gcg ttg ctc gct 1472 His Leu Asp Tyr Ile Thr Glu Lys Leu
Trp Arg Asn Ala Leu Leu Ala 420 425 430 ggg gcg gtg ccg gtg gtg ctg
ggc cca gac cgt gcc aac tac gag cgc 1520 Gly Ala Val Pro Val Val
Leu Gly Pro Asp Arg Ala Asn Tyr Glu Arg 435 440 445 ttt gtg ccc cgc
ggc gcc ttc atc cac gtg gac gac ttc cca agt gcc 1568 Phe Val Pro
Arg Gly Ala Phe Ile His Val Asp Asp Phe Pro Ser Ala 450 455 460 465
tcc tcc ctg gcc tcg tac ctg ctt ttc ctc gac cgc aac ccc gcg gtc
1616 Ser Ser Leu Ala Ser Tyr Leu Leu Phe Leu Asp Arg Asn Pro Ala
Val 470 475 480 tat cgc cgc tac ttc cac tgg cgc cgg agc tac gct gtc
cac atc acc 1664 Tyr Arg Arg Tyr Phe His Trp Arg Arg Ser Tyr Ala
Val His Ile Thr 485 490 495 tcc ttc tgg gac gag cct tgg tgc cgg gtg
tgc cag gct gta cag agg 1712 Ser Phe Trp Asp Glu Pro Trp Cys Arg
Val Cys Gln Ala Val Gln Arg 500 505 510 gct ggg gac cgg ccc aag agc
ata cgg aac ttg gcc agc tgg ttc gag 1760 Ala Gly Asp Arg Pro Lys
Ser Ile Arg Asn Leu Ala Ser Trp Phe Glu 515 520 525 cgg tga
agccgcgctc ccctggaagc gacccagggg aggccaagtt gtcagctttt 1816 Arg *
530 tgatcctcta ctgtgcatct ccttgactgc cgcatcatgg gagtaagttc
ttcaaacacc 1876 catttttgct ctatgggaaa aaaacgattt accaattaat
attactcagc acagagatgg 1936 gggcccggtt tccatatttt ttgcacagct
agcaattggg ctccctttgc tgctgatggg 1996 catcattgtt taggggtgaa
ggagggggtt cttcctcacc ttgtaaccag tgcagaaatg 2056 aaatagctta
gcggcaagaa gccgttgagg cggtttcctg aatttcccca tctgccacag 2116
gccatatttg tggcccgtgc agcttccaaa tctcatacac aactgttccc gattcacgtt
2176 tttctggacc aaggtgaagc aaatttgtgg ttgtagaagg agccttgttg
gtggagagtg 2236 gaaggactgt ggctgcaggt gggactttgt tgtttggatt
cctcacagcc ttggctcctg 2296 agaaaggtga ggagggcagt ccaagagggg
ccgctgactt ctttcacaag tactatctgt 2356 tcccctgtcc tgtgaatgga
agcaaagtgc tggattgtcc ttggaggaaa cttaagatga 2416 atacatgcgt
gtacctcact ttacataaga aatgtattcc tgaaaagctg catttaaatc 2476
aagtcccaaa ttcattgact taggggagtt cagtatttaa tgaaacccta tggagaattt
2536 atccctttac aatgtgaata gtcatctcct aatttgtttc ttctgtcttt
atgtttttct 2596 ataacctgga ttttttaaat catattaaaa ttacagatgt
gaaaataaag cagaagcaac 2656 ctttttccct cttcccagaa aaccagtctg
tgtttacaga cagaagagaa ggaagccata 2716 gtgtcacttc cacacaatta
tttatttcat gtctttactg gacctgaaat ttaaactgca 2776 atgccagtcc
tgcaggagtg ctggcattac cctctgcaga acagtgaaag gtattgcact 2836
acattatgga atcatgcaaa aaaaa 2861 13 530 PRT Homo Sapien 13 Met Arg
Arg Leu Trp Gly Ala Ala Arg Lys Pro Ser Gly Ala Gly Trp 1 5 10 15
Glu Lys Glu Trp Ala Glu Ala Pro Gln Glu Ala Pro Gly Ala Trp Ser 20
25 30 Gly Arg Leu Gly Pro Gly Arg Ser Gly Arg Lys Gly Arg Ala Val
Pro 35 40 45 Gly Trp Ala Ser Trp Pro Ala His Leu Ala Leu Ala Ala
Arg Pro Ala 50 55 60 Arg His Leu Gly Gly Ala Gly Gln Gly Pro Arg
Pro Leu His Ser Gly 65 70 75 80 Thr Ala Pro Phe His Ser Arg Ala Ser
Gly Glu Arg Gln Arg Arg Leu 85 90 95 Glu Pro Gln Leu Gln His Glu
Ser Arg Cys Arg Ser Ser Thr Pro Ala 100 105 110 Asp Ala Trp Arg Ala
Glu Ala Ala Leu Pro Val Arg Ala Met Gly Ala 115 120 125 Pro Trp Gly
Ser Pro Thr Ala Ala Ala Gly Gly Arg Arg Gly Trp Arg 130 135 140 Arg
Gly Arg Gly Leu Pro Trp Thr Val Cys Val Leu Ala Ala Ala Gly 145 150
155 160 Leu Thr Cys Thr Ala Leu Ile Thr Tyr Ala Cys Trp Gly Gln Leu
Pro 165 170 175 Pro Leu Pro Trp Ala Ser Pro Thr Pro Ser Arg Pro Val
Gly Val Leu 180 185 190 Leu Trp Trp Glu Pro Phe Gly Gly Arg Asp Ser
Ala Pro Arg Pro Pro 195 200 205 Pro Asp Cys Arg Leu Arg Phe Asn Ile
Ser Gly Cys Arg Leu Leu Thr 210 215 220 Asp Arg Ala Ser Tyr Gly Glu
Ala Gln Ala Val Leu Phe His His Arg 225 230 235 240 Asp Leu
Val Lys Gly Pro Pro Asp Trp Pro Pro Pro Trp Gly Ile Gln 245 250 255
Ala His Thr Ala Glu Glu Val Asp Leu Arg Val Leu Asp Tyr Glu Glu 260
265 270 Ala Ala Ala Ala Ala Glu Ala Leu Ala Thr Ser Ser Pro Arg Pro
Pro 275 280 285 Gly Gln Arg Trp Val Trp Met Asn Phe Glu Ser Pro Ser
His Ser Pro 290 295 300 Gly Leu Arg Ser Leu Ala Ser Asn Leu Phe Asn
Trp Thr Leu Ser Tyr 305 310 315 320 Arg Ala Asp Ser Asp Val Phe Val
Pro Tyr Gly Tyr Leu Tyr Pro Arg 325 330 335 Ser His Pro Gly Asp Pro
Pro Ser Gly Leu Ala Pro Pro Leu Ser Arg 340 345 350 Lys Gln Gly Leu
Val Ala Trp Val Val Ser His Trp Asp Glu Arg Gln 355 360 365 Ala Arg
Val Arg Tyr Tyr His Gln Leu Ser Gln His Val Thr Val Asp 370 375 380
Val Phe Gly Arg Gly Gly Pro Gly Gln Pro Val Pro Glu Ile Gly Leu 385
390 395 400 Leu His Thr Val Ala Arg Tyr Lys Phe Tyr Leu Ala Phe Glu
Asn Ser 405 410 415 Gln His Leu Asp Tyr Ile Thr Glu Lys Leu Trp Arg
Asn Ala Leu Leu 420 425 430 Ala Gly Ala Val Pro Val Val Leu Gly Pro
Asp Arg Ala Asn Tyr Glu 435 440 445 Arg Phe Val Pro Arg Gly Ala Phe
Ile His Val Asp Asp Phe Pro Ser 450 455 460 Ala Ser Ser Leu Ala Ser
Tyr Leu Leu Phe Leu Asp Arg Asn Pro Ala 465 470 475 480 Val Tyr Arg
Arg Tyr Phe His Trp Arg Arg Ser Tyr Ala Val His Ile 485 490 495 Thr
Ser Phe Trp Asp Glu Pro Trp Cys Arg Val Cys Gln Ala Val Gln 500 505
510 Arg Ala Gly Asp Arg Pro Lys Ser Ile Arg Asn Leu Ala Ser Trp Phe
515 520 525 Glu Arg 530 14 19 DNA Artificial Sequence NotI-BstXI
linker/adaptor 14 gcggccgctt tagagcaca 19 15 15 DNA Artificial
Sequence NotI-BstXI linker-adaptor 15 ctctaaagcg gccgc 15 16 21 DNA
Artificial Sequence Synthesized oligonucleotide 16 tgtgaagctc
cctaaattcc c 21 17 14 DNA Artificial Sequence Oligonucleotide ACE
175 17 tcgacgcggc cgcg 14 18 10 DNA Homo Sapien 18 ggggatttcc 10 19
10 DNA Homo Sapien 19 agggatttcc 10 20 10 DNA Homo Sapien 20
ggggaaaccc 10 21 32 DNA Artificial Sequence VCAM1 3' primer
sequence 21 gagctcgagg ccgcaccatg cctgggaaga tg 32 22 23 DNA
Artificial Sequence VCAM1 5' primer sequence 22 ctagctagcg
cgttttactt cac 23 23 23 DNA Artificial Sequence NheI-Hind III
linker 23 ctagctttcc aaggtgagtc cta 23 24 23 DNA Artificial
Sequence NheI-Hind III linker 24 agcttaggac tcaccttgga aag 23 25 15
DNA Artificial Sequence VCAM1 antisense oligomer 25 cccaggcatt
ttaag 15 26 27 DNA Homo Sapien 26 augccuggga agauggucgu gauccuu 27
27 18 DNA Homo Sapien 27 aagauggucg ugauccuu 18 28 39 DNA
Artificial Sequence VCAM1 mRNA recognition sequence 28 aaggaucacc
ugaugagucc gugaggacga aaccaucuu 39
* * * * *