U.S. patent application number 11/526349 was filed with the patent office on 2007-01-25 for system and method of aerosolized agent capture and detection.
Invention is credited to William C. Craig.
Application Number | 20070019193 11/526349 |
Document ID | / |
Family ID | 33417480 |
Filed Date | 2007-01-25 |
United States Patent
Application |
20070019193 |
Kind Code |
A1 |
Craig; William C. |
January 25, 2007 |
System and method of aerosolized agent capture and detection
Abstract
System and method of aerosolized agent detection. Multi-part
collection tape is employed for capturing particles of interest for
immediate analysis and optional forensic preservation and recording
information related to capture and subsequent real-time analysis.
Laser induced breakdown spectroscopic processing is employed and
spectra attributable to known collection tape materials is
subtracted to derive particle spectra for comparison to known
hazardous agent spectra.
Inventors: |
Craig; William C.; (Endwell,
NY) |
Correspondence
Address: |
BURNS & LEVINSON, LLP;(FORMERLY PERKINS SMITH & COHEN LLP)
125 SUMMER STREET
BOSTON
MA
02110
US
|
Family ID: |
33417480 |
Appl. No.: |
11/526349 |
Filed: |
September 25, 2006 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
10437962 |
May 14, 2003 |
7113277 |
|
|
11526349 |
Sep 25, 2006 |
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Current U.S.
Class: |
356/318 ;
356/36 |
Current CPC
Class: |
G01N 2021/8585 20130101;
G01N 21/718 20130101; G01N 21/13 20130101; G01N 2021/845 20130101;
G01N 2021/8494 20130101; G01N 15/0637 20130101 |
Class at
Publication: |
356/318 ;
356/036 |
International
Class: |
G01J 3/30 20060101
G01J003/30; G01N 1/00 20060101 G01N001/00 |
Claims
1. For use with an aerosolized agent detector providing a particle
stream for analysis, a vaporizing real-time analyzer, and an
encoder for storing data relative to the fluid samples collection
and analysis, a sample capture tape, comprising: a vaporizable
first collecting portion designed to collect for immediate analysis
selected particles from an aerosol stream potentially containing
aerosolized agents; a second collecting portion designed to collect
for preservation selected particles from the aerosol stream; and a
magnetic portion providing storage of information related to the
capture and analysis of the aerosol stream.
2. The sample capture tape of claim 1, further comprising: a
preservation portion providing a sterile enclosure for particles
collected on the second collecting portion.
Description
RELATED APPLICATIONS
[0001] The present application is a divisional of U.S. patent
application Ser. No. 10/437,962, filed May 14, 2003 and hereby
claims priority thereof.
BACKGROUND OF THE INVENTION
[0002] The present invention relates generally to the capture and
detection of aerosolized agents, such as, for example, chemical or
biological biowarfare agents, and, more particularly to systems for
and methods of continuous sampling and analysis of aerosol samples
potentially containing such agents.
[0003] As the anthrax mailings of October of 2001 demonstrated, a
number of vectors are available to the terrorist bent on social
disruption. Biological warfare agents have long been a domestic
terror concern and the use of the mails as a delivery method has
many serious consequences. Not only are mail recipients at risk,
but all those who handle or are in the vicinity of the mail piece
during processing are at risk as well. In the extreme case, a few
strategically posted items could bring a nation's postal
administration, such as the United States Postal Service, to a
complete halt. If mail delivery were to cease, even for a few days,
the impact to trade, commerce, finance and general communication is
incalculable.
[0004] Thus, it is desirable to capture and identify aerosolized
particulate matter that may issue from sources requiring
monitoring. Such sources could include mail pieces being sorted or
otherwise processed, building ventilation systems, import and
export cargo and military point detection systems.
[0005] Prior attempts to detect and identify pathogens in the mail
use chemical or bio fluorescence methods. An example of a chemical
method is Polymerase Chain Reaction (PCR). PCR is a DNA
amplification technique that has been used successfully in
laboratory environments in recent years. PCR is a powerful and
useful technique but it does not generate results in real-time. The
fastest PCR systems require a minimum of 30-60 minutes to process a
sample and render a result. This time lag is problematic, as the
mail piece containing the hazard will have traveled further down
stream potentially affecting postal workers or postal customers.
Other techniques using multi spectral ultra-violet (UV)
fluorescence techniques may render a result quickly, but are prone
to false alarms and are often non-specific relative to the threat.
For example, non-threatening biological particulate in the right
size range will typically cause a UV sensor system to indicate a
false positive detection. Also, even if hazardous particles are
correctly detected, UV based sensors will typically indicate the
presence of the threat without specifically identifying the
agent.
SUMMARY OF THE INVENTION
[0006] The present invention describes systems for and methods of
capturing and rapidly identifying aerosolized particulate matter in
fluid samples to be analyzed, such as, for example, hazardous
agents issued from mail pieces during sorting and other processing
steps. The invention is applicable to a range of uses outside of
the mailing industry, including building ventilation systems,
checking import and export cargo by customs officials and military
point detection systems. The system provides this timely
notification without the possible false alarms inherent in many of
the systems described above.
[0007] The present invention provides a sample capture tape and an
aerosolized agent detector employing the tape, the detector
including a dispenser having a fluid sample input port and an exit
port for providing a concentrated particle stream, an analyzer
providing real-time detection of aerosolized agents, and a means
for advancing a section of the tape from the vicinity of the
dispenser exit port to the analyzer. The sample capture tape
includes a data storage portion, and the tape is oriented and
configured to receive the particle stream from the dispenser exit
port and collect selected particles on one or more collecting
portions. An encoder stores information relative to the fluid
samples' collection and analysis on the data storage portion.
[0008] The one or more sample collection portions of the capture
tape further comprises an immediate analysis portion and an
optional sample preservation portion. A wide variety of alternate
approaches are available in capturing the desired particles. For
example, the sample collection portions may be comprised of
permeable filter, adhesive-coated or electrically charged
materials, or may be comprised of a material with a surface
incorporating microscopic features designed to capture particles
impinged thereupon. Other capturing mechanisms will be readily
apparent to artisans and are deemed to be within the scope of the
present invention.
[0009] The particle stream exiting the dispenser preferably, though
not exclusively, impinges nearly perpendicularly on the sample
collection portions of the sample collection tape. In one preferred
embodiment, the dispenser further comprises a virtual impactor for
pre-concentrating the particle stream. As will be described in
detail below, various configurations are allowed for in which the
particle stream impinges from either side of the sample capture
tape.
[0010] In certain embodiments, the means for advancing the sample
capture tape comprises a reel-to-reel like system, wherein the
sample capture tape is provided on a source reel and a take-up reel
pulls the tape at the desired rate, or in indexed intervals,
through the path of the particle stream. The collection process
can, thus, continue in an uninterrupted fashion even as the sample
capture tape is in the process of advancing. A sterile tape reel
may additionally be employed to interleave a sterile enclosing
layer between the sample collection tape layers on the take-up
reel.
[0011] The analyzer, in preferred embodiments, is comprised of a
laser-induced breakdown spectroscopic (LIBS) system adapted to
differentiate between spectra elements attributable to portions of
vaporized sample capture tape and spectra elements attributable to
various potentially hazardous agent particles.
[0012] The present invention provides a method of continuous
detection of aerosolized agents comprising the steps of: providing
a source of sample capture tape comprised of at least a first
collection portion and a data storage portion; collecting particles
by impinging a particle-laden aerosol stream on the first
collection portion; advancing the first collection portion from the
particle stream to an analyzer; analyzing in real-time the
particles collected on the first collection portion; and
electronically encoding information on the data storage portion
related to the sample collection and analysis. The method
efficiency is enhanced by optionally pre-concentrating the fluid to
be analyzed to form the particle-laden aerosol stream. The
advancement of the sample capture tape allows the collection
process to continue in an uninterrupted fashion, and
synchronization between collection and analysis allows continuous
detection.
[0013] The method may further include the step of collecting
samples from the particle-laden stream on a second collection
portion of the sample capture tape at a time nearly simultaneous
with the sample collecting on the first portion, and preserving the
samples collected on the second collection portion. The
preservation may be effected by covering the particles collected on
the second collection portion with a sterile tape, such as an
interleaving reel-to-reel configuration as described in more detail
below.
BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING
[0014] FIG. 1 is diagrammatic view of a an aerosolized agent
detecting system configured for use with mail processing
equipment.
[0015] FIG. 2 is an isometric schematic diagram of an aerosolized
agent detecting system employing an optional sterile tape.
[0016] FIG. 3A is an isometric view of sample capture tape in roll
form depicting multiple portions thereof.
[0017] FIGS. 3B-D are diagrammatic views of various embodiments of
materials usable as collection portions of the sample capture
tape.
[0018] FIG. 4 is a flow diagram of the operational functions of an
embodiment of the present invention.
[0019] FIG. 5 is an isometric schematic diagram of an alternate
collection mechanism designed for continuous collection to
eliminate the possibility that an event might be lost during the
collection process.
DETAILED DESCRIPTION OF THE INVENTION
[0020] Certain preferred embodiments of the present invention will
now be described with reference to the figures identified
above.
[0021] The present invention is now described more fully. For the
purpose of illustration only, the present invention is described in
embodiments configured for use in a mail testing system. As
discussed above, the aerosol agent detection system and method are
useful in a variety of testing and/or monitoring environments.
Referring to FIG. 1, a mail testing system 10 is attached to a
conventional piece of mail processing equipment, 12. In order to
transport and sort a mail piece, the processing equipment 12
typically pinches an envelope tightly between two flat transport
belts (not shown) moving at high speed. An induction point into the
mail processor is called a feeder 13, and at this point an envelope
is first pinched between the transport belts. As the envelope is
pinched, air is forced from the envelope and with it any
particulate matter contained therein. Aerosol collection sample
tube 14, which is under vacuum, captures air forced from the
envelope. A virtual impactor 16 dispenses a pre-concentrated
particle stream by separating smaller aerosolized particles from
larger ones contained in the air delivered through collection tube
14.
[0022] The smaller particles of interest are then impinged on a
collection tape 18, which captures a sufficient number of them to
perform testing and, in certain preferred embodiments, sample
preservation. After a predefined collection period, collection tape
18 is indexed to an adjacent location in order that a new sample of
the air stream may be impinged upon a fresh portion of the
collection tape. A Laser Induced Breakdown Spectroscopy (LIBS)
method is used to analyze the particles collected on collection
tape 18.
[0023] The LIBS process requires that the particulate matter
collected on the tape 18 be vaporized using a high-energy laser 20.
The electromagnetic spectrum radiated as a result of the
vaporization of the collected particles and the portion of tape 18
upon which they are collected is captured by optics 22 and
transmitted to a spectrometer 24, such as the Ocean Optics
LIBS2000+ Broad Band Spectrometer. Spectrometer 24 interprets the
relative intensity of the radiation at all frequencies in the band
of interest--typically 200-980 nm and generates a spectral curve.
The spectral curve is then sent to control computer 26 for
analysis. In order to protect personnel in the vicinity of system
10, collected air is exhausted through a High Efficiency Particle
Arresting (HEPA) filter 28 under the impetus of blower 29. Control
computer 26 compares the spectral curve to a library of spectral
curves for known biohazards and typical hoax or naturally occurring
powders. Control computer 26 may also subtract from the spectral
curve spectral elements known to be attributable to the portion of
vaporized tape 18.
[0024] If the control computer 26 detects a high likelihood of a
biohazard event, a visual, audio and/or process control indication
of the detection is provided. In the system depicted, event
detection is indicated by turning a status light 30 on and invoking
an E-Stop interface 32 for cessation of mail processing. If
multiple systems are implemented in a single building, a central
control system 34 may provide overall supervisory control and
monitoring functions for all systems. In the embodiment depicted,
central control system 34 is comprised of an Ethernet interface 35,
a control computer 36, various peripherals including a printer 40,
uninterruptible power supply (UPS) 42, an E-Stop switch 44, and a
reset switch 46. A maintenance paging system 38 can also be
integrated to alert site personnel if a bio detection event has
occurred or if maintenance action is required.
[0025] A more detailed view of testing system 10 can be seen in
FIG. 2.
[0026] A specifically designed collection tape 50 is arranged in a
reel-to-reel configuration as shown with a source reel 86 and a
take-up reel 78. Collection tape 50, as will be described in
further detail below, is comprised of at least a first collection
section 88 for particle collection for immediate analysis and a
data encoding region 82, but preferably further comprises a second
collection section 90. The two collection sections 88,90 of the
particular configuration of system 10 depicted are used for
particulate collection; one is designated the LIBS collection
section 88 and is used for analysis of the particulate deposited
thereon, and the other forensic section 90 for preserving part of
the collected particle sample for forensic purposes. Encoding
region 82 of tape 50 may be comprised of any number of mechanical
and/or electrical encoding media, but is shown here as a magnetic
strip for data storage along an edge of the tape.
[0027] Virtual impactor 16 receives an aerosolized particle stream
54, separates unwanted particles 56 based on particle size, and
dispenses an aerosolized stream laden with particles of interest
58. The use of virtual impactor 16 raises the efficiency of testing
system 10, but it is not necessary to all embodiments of the
present invention. Any dispenser capable of delivering a particle
stream derived from a fluid sample is suitable for use. Referring
again to FIG. 2, the particles of interest 58 are impinged on
collection tape 50 while at Position A 59.
[0028] After a predetermined collection period, the section of
collection tape impinged upon is indexed to Position B 61. A
portion of the particles 58 at Position B is then vaporized using
laser 20. The resulting spectral emission is captured by the optics
22, which focus the emitted light for transmission to the
spectrometer 24 through a fiber optic link 74. After the spectral
characteristics are quantified by the spectrometer, spectral data
is then sent to control computer 26 for analysis. Control computer
26 employs pattern-matching techniques to identify the particulate
matter impinged and collected on collection tape 50.
[0029] The invention also includes a means for advancing or
indexing the tape relative to the impinging particle stream, laser
20 and optics 22. Although a number of mechanisms could be
employed, the advancing means is depicted as a reel-to-reel
configuration. As the tape is indexed, it is stored on the take-up
reel 78. To prevent particles from being transferred between tape
layers on take-up reel 78, a sterile tape 80 is wound between the
layers of collection tape. This creates a closed environment for
effectively preserving any particles captured on the tape. After
processing the spectral information, and as the tape is indexed,
control computer 26 writes parametric information on the magnetic
strip comprising the encoding region 82 in this embodiment, which
is situated along the edge of the collection tape, using magnetic
write head 84. The magnetic write head 84 is illustrated as
positioned between the impinging stream and the detection optics,
but alternatively could be located further along the trajectory of
the tape, or may even be comprised of multiple write heads. The
type of data that may be encoded may include information related to
the source of aerosolized particle stream 54, time/date of
collection, identity and/or location of equipment employed in
capture and analysis, and the results of analysis, such as the
spectral information captured, results of the pattern matching
analysis, etc.
[0030] The collection tape 50, as shown in FIG. 3A as supplied on a
source reel 86, is comprised of three distinct sections; the
encoding region 82 (e.g., magnetic edge), the LIBS collection
section 88, and the forensic section 90. The magnetic embodiment of
encoding region 82 is designed to store digital information about
when and where the LIBS analysis was done, and the results of the
analysis. Any other storage media (e.g., optical, mechanical, etc.)
suitable for quick recording of information can be suitably
employed in the encoding region. The LIBS and forensic sections of
the tape are specifically designed to capture the aerosolized
particles impinged thereon. A number of design alternatives are
suitable for use as the collection sections 88,90. FIG. 3B
illustrates an embodiment of a collection tape 100 comprised of a
porous filter type material designed to trap particles as the air
passes through the material. The tape 100 may have an adhesive
coating designed to stick to the particles impinged on its surface.
FIG. 3C illustrates an embodiment of a collection tape 102 having a
surface engineered with microscopic hooks or protrusions that will
tend to trap particles. As another alternative, FIG. 3D shows a
collection tape 104 having a charged surface that attracts
particles of interest that have passed through an electrostatic
field imparting the opposite charge to them. As stated above,
forensic section 90 allows material to be retained for confirmation
testing using alternative methods and for legal evidence.
[0031] Referring again to FIG. 2, regardless of how the particles
are captured, the LIBS collection section 88 is reserved for LIBS
processing, which requires the particles in the section 88 be
vaporized by the high-energy laser 20. The resulting spectra are
captured for analysis. Control computer 26 performs the analysis,
subtracting the known spectral characteristics of the collection
tape from the spectral information captured. This is done to
isolate the signal of the particulate from that of the tape
substrate.
[0032] A flow chart illustrating a preferred method of using system
10 is shown in FIG. 4. The first step is the particulate collection
process 106. The length of time required for the collection is a
programmable parameter, which will depend on several factors
related to the application of the invention, including the
background particulate loading, the expected duration of a
bio-release event, and the expected biohazard particulate
concentration of the event in terms of agent containing particles
per liter of air (ACPLA). For a postal application, the expected
release will have high ACPLA count over a very short duration. The
dwell time for the postal application is therefore short, on the
order of 1 to 10 minutes. For open-air environment point detection
applications, where much lower ACPLA is expected, the dwell time
may be 30-90 minutes or more.
[0033] In step 108, after collecting particulate for the specified
dwell time, the tape is indexed. When the tape is indexed, a fresh
section of the tape is moved into position for particulate
collection. Also, as the tape is indexed, the portion of the tape
most recently exposed to the aerosolized particle stream for the
programmed dwell time is moved into position for LIBS analysis. In
step 110, LIBS processing requires that a portion of the collection
tape be exposed to a high-energy laser, for example the ULTRA CFR
Nd:YAG laser from Big Sky Laser Technologies. When the laser is
activated, the high-temperature of the laser focused on the LIBS
portion of the collection tape creates plasma. As the plasma cools,
the excited atoms in the plasma emit light at wavelengths
characteristic of the elements contained in the particulate sample
on the tape, as well as the elemental constituents of the tape
itself. Since the tape is a known quantity previously characterized
using the LIBS process, the spectral elements of the tape can be
algorithmically subtracted, in step 112, from the captured plasma
spectrum.
[0034] In step 114, the resulting differential spectrum is then
used for the signal processing and pattern matching process. The
pattern matching process first resolves the captured differential
spectrum into a set of characteristic features. These features are
then compared to a library of feature sets for known biohazards
previously characterized using the LIBS process and a similar
apparatus. Several established methods exist for the
pattern-matching algorithm including neural network and least sum
of squares techniques. The output of the pattern matching process
is a set of probabilities for each of the library substances the
captured spectrum is tested against. In step 116, the probabilities
are compared to one or more predefined thresholds for a
determination whether a hazardous agent has been detected.
[0035] If any of the probabilities exceed the one or more
predefined thresholds, an alarm condition exists. If an alarm
condition exists, an alarm protocol is initiated in step 120. The
protocol will be defined by the user of the technology, but will
typically include shutting down any associated equipment,
activating appropriate audio/visual alarms and initiating events
such as paging key personnel and notifying the appropriate first
responder agency. The method of communication can be via e-mail,
telephone messages, pager messages, or a combination, based on the
user's preference. All information relative to the bio-detection
event is recorded on the encoding region 82 of the collection tape
50. If, after processing the differential spectrum, an alarm
condition does not exist, the results of the spectrum analysis are
still encoded on the encoding region of the tape, and the process
begins again as determined by the programmed collection
interval.
[0036] In another embodiment of the invention, the collection
process occurs in a continuous fashion, without any interruptions
that might be introduced in indexer-based configurations. Referring
to FIG. 5, a continuous collection device 126 is comprised of a
rotating pulley 128 on a hollow shaft 130. A particle-containing
air stream 132 enters through the hollow shaft 130. For a half of
the rotation of pulley 128, air stream 132 follows path 134 through
outlet port 144 to impinging point 138. For the other 180 degrees
of rotation, port 144 is effectively closed as a result of its
adjacency to sealing surface 142. During this interval, port 146,
which is 180 degrees out of phase with port 144, is simultaneously
open and air stream 132 will follow path 148 to impinge upon a
different portion of collection tape 50. This mechanism allows
sample collection to be performed in a continuous fashion,
eliminating the possibility of missing a very short duration
release as the tape is indexed. Collected particulate 150 are then
processed as described in proceeding paragraphs. Depending on the
design of the ports incorporated into the pulley 128, air stream
132 can be either draw through the collection tape if the tape is
made from a porous material mesh, such as depicted in FIG. 3B, or
it can be impinged against collection tapes comprised of solid
materials with particle-arresting surface features, such as shown
in FIGS. 3C and 3D.
[0037] Although the invention has been described with respect to
various embodiments, it should be realized this invention is also
capable of a wide variety of further and other embodiments within
the spirit and scope of the appended claims.
* * * * *