U.S. patent application number 11/314171 was filed with the patent office on 2006-09-14 for genetic control of flowering. This patent application is currently assigned to PIONEER HI-BRED INTERNATIONAL, INC.. Invention is credited to George M. Coupland, Joanna J. Putterill.
Application Number | 20060206967 11/314171 |
Document ID | / |
Family ID | 10763764 |
Filed Date | 2006-09-14 |
United States Patent Application | 20060206967 |
Kind Code | A1 |
Coupland; George M. ; et al. | September 14, 2006 |
The CONSTANS(CO) gene of Arabidopsis thaliana and homologues from Brassica napus are provided and are useful for influencing flowering characteristics in transgenic plants, especially the timing of flowering.
Inventors: | Coupland; George M.; (Colney, GB) ; Putterill; Joanna J.; (Auckland, NZ) |
Correspondence Address: |
NIXON & VANDERHYE, PC 901 NORTH GLEBE ROAD, 11TH FLOOR ARLINGTON VA 22203 US |
Assignee: | PIONEER HI-BRED INTERNATIONAL,
INC. Johnston IA |
Family ID: | 10763764 |
Appl. No.: | 11/314171 |
Filed: | December 22, 2005 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
10143849 | May 14, 2002 | |||
11314171 | Dec 22, 2005 | |||
09516191 | Mar 1, 2000 | |||
10143849 | May 14, 2002 | |||
08945056 | Oct 20, 1997 | 6077994 | ||
PCT/GB95/02561 | Nov 1, 1995 | |||
09516191 | Mar 1, 2000 | |||
Current U.S. Class: | 800/290 ; 435/419; 435/468; 536/23.6 |
Current CPC Class: | C12N 15/8262 20130101; C12N 15/827 20130101; C12N 15/8261 20130101; C07K 14/415 20130101; Y02A 40/146 20180101 |
Class at Publication: | 800/290 ; 435/419; 435/468; 536/023.6 |
International Class: | A01H 1/00 20060101 A01H001/00; C07H 21/04 20060101 C07H021/04; C12N 15/82 20060101 C12N015/82; C12N 5/04 20060101 C12N005/04 |
Date | Code | Application Number |
---|---|---|
Nov 2, 1994 | GB | 9422083.7 |
Sequence CWU 0
0
SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF
SEQUENCES: 33 (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 1124 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
cDNA to mRNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi)
ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis thaliana (B) STRAIN:
Landsberg erecta (viii) POSITION IN GENOME: (A) CHROMOSOME/SEGMENT:
chromosome 5 (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1119
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: ATG TTG AAA CAA GAG AGT
AAC GAC ATA GGT AGT GGA GAG AAC AAC AGG 48 Met Leu Lys Gln Glu Ser
Asn Asp Ile Gly Ser Gly Glu Asn Asn Arg 1 5 10 15 GCA CGA CCC TGT
GAC ACA TGC CGG TCA AAC GCC TGC ACC GTG TAT TGC 96 Ala Arg Pro Cys
Asp Thr Cys Arg Ser Asn Ala Cys Thr Val Tyr Cys 20 25 30 CAT GCA
GAT TCT GCC TAC TTG TGC ATG AGC TGT GAT GCT CAA GTT CAC 144 His Ala
Asp Ser Ala Tyr Leu Cys Met Ser Cys Asp Ala Gln Val His 35 40 45
TCT GCC AAT CGC GTT GCT TCC CGC CAT AAA CGT GTC CGG GTC TGC GAG 192
Ser Ala Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys Glu 50
55 60 TCA TGT GAG CGT GCT CCG GCT GCT TTT TTG TGT GAG GCA GAT GAT
GCC 240 Ser Cys Glu Arg Ala Pro Ala Ala Phe Leu Cys Glu Ala Asp Asp
Ala 65 70 75 80 TCT CTA TGC ACA GCC TGT GAT TCA GAG GTT CAT TCT GCA
AAC CCA CTT 288 Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala
Asn Pro Leu 85 90 95 GCT AGA CGC CAT CAG CGA GTT CCA ATT CTA CCA
ATT TCT GGA AAC TCT 336 Ala Arg Arg His Gln Arg Val Pro Ile Leu Pro
Ile Ser Gly Asn Ser 100 105 110 TTC AGC TCC ATG ACC ACT ACT CAC CAC
CAA AGC GAG AAA ACA ATG ACC 384 Phe Ser Ser Met Thr Thr Thr His His
Gln Ser Glu Lys Thr Met Thr 115 120 125 GAT CCA GAG AAG AGA CTG GTG
GTG GAT CAA GAG GAA GGT GAA GAA GGT 432 Asp Pro Glu Lys Arg Leu Val
Val Asp Gln Glu Glu Gly Glu Glu Gly 130 135 140 GAT AAG GAT GCC AAG
GAG GTT GCT TCG TGG CTG TTC CCT AAT TCA GAC 480 Asp Lys Asp Ala Lys
Glu Val Ala Ser Trp Leu Phe Pro Asn Ser Asp 145 150 155 160 AAA AAT
AAC AAT AAC CAA AAC AAT GGG TTA TTG TTT AGT GAT GAG TAT 528 Lys Asn
Asn Asn Asn Gln Asn Asn Gly Leu Leu Phe Ser Asp Glu Tyr 165 170 175
CTA AAC CTT GTG GAT TAC AAC TCG AGT ATG GAC TAC AAA TTC ACA GGT 576
Leu Asn Leu Val Asp Tyr Asn Ser Ser Met Asp Tyr Lys Phe Thr Gly 180
185 190 GAA TAC AGT CAA CAC CAA CAA AAC TGC AGC GTA CCA CAG ACG AGC
TAC 624 Glu Tyr Ser Gln His Gln Gln Asn Cys Ser Val Pro Gln Thr Ser
Tyr 195 200 205 GGG GGA GAT AGA GTT GTT CCG CTT AAA CTT GAA GAA TCA
AGG GGC CAC 672 Gly Gly Asp Arg Val Val Pro Leu Lys Leu Glu Glu Ser
Arg Gly His 210 215 220 CAG TGC CAT AAC CAA CAG AAT TTT CAG TTC AAT
ATC AAA TAT GGC TCC 720 Gln Cys His Asn Gln Gln Asn Phe Gln Phe Asn
Ile Lys Tyr Gly Ser 225 230 235 240 TCA GGG ACT CAC TAC AAC GAC AAT
GGT TCC ATT AAC CAT AAC GCA TAC 768 Ser Gly Thr His Tyr Asn Asp Asn
Gly Ser Ile Asn His Asn Ala Tyr 245 250 255 ATT TCA TCC ATG GAA ACT
GGT GTT GTG CCG GAG TCA ACA GCA TGT GTC 816 Ile Ser Ser Met Glu Thr
Gly Val Val Pro Glu Ser Thr Ala Cys Val 260 265 270 ACA ACA GCT TCA
CAC CCA AGA ACG CCC AAA GGG ACA GTA GAG CAA CAA 864 Thr Thr Ala Ser
His Pro Arg Thr Pro Lys Gly Thr Val Glu Gln Gln 275 280 285 CCT GAC
CCT GCA AGC CAG ATG ATA ACA GTA ACA CAA CTC AGT CCA ATG 912 Pro Asp
Pro Ala Ser Gln Met Ile Thr Val Thr Gln Leu Ser Pro Met 290 295 300
GAC AGA GAA GCC AGG GTC CTG AGA TAC AGA GAG AAG AGG AAG ACA AGG 960
Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Arg Lys Thr Arg 305
310 315 320 AAA TTT GAG AAG ACA ATA AGG TAT GCT TCG AGG AAG GCA TAT
GCA GAG 1008 Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala
Tyr Ala Glu 325 330 335 ATA AGA CCG CGG GTC AAT GGC CGG TTC GCA AAG
AGA GAA ATC GAA GCC 1056 Ile Arg Pro Arg Val Asn Gly Arg Phe Ala
Lys Arg Glu Ile Glu Ala 340 345 350 GAG GAG CAA GGG TTC AAC ACG ATG
CTA ATG TAC AAC ACA GGA TAT GGG 1104 Glu Glu Gln Gly Phe Asn Thr
Met Leu Met Tyr Asn Thr Gly Tyr Gly 355 360 365 ATT GTT CCT TCA TTC
TGATA 1124 Ile Val Pro Ser Phe 370 (2) INFORMATION FOR SEQ ID NO:
2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 373 amino acids (B)
TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: Met Leu Lys Gln Glu Ser
Asn Asp Ile Gly Ser Gly Glu Asn Asn Arg 1 5 10 15 Ala Arg Pro Cys
Asp Thr Cys Arg Ser Asn Ala Cys Thr Val Tyr Cys 20 25 30 His Ala
Asp Ser Ala Tyr Leu Cys Met Ser Cys Asp Ala Gln Val His 35 40 45
Ser Ala Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys Glu 50
55 60 Ser Cys Glu Arg Ala Pro Ala Ala Phe Leu Cys Glu Ala Asp Asp
Ala 65 70 75 80 Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala
Asn Pro Leu 85 90 95 Ala Arg Arg His Gln Arg Val Pro Ile Leu Pro
Ile Ser Gly Asn Ser 100 105 110 Phe Ser Ser Met Thr Thr Thr His His
Gln Ser Glu Lys Thr Met Thr 115 120 125 Asp Pro Glu Lys Arg Leu Val
Val Asp Gln Glu Glu Gly Glu Glu Gly 130 135 140 Asp Lys Asp Ala Lys
Glu Val Ala Ser Trp Leu Phe Pro Asn Ser Asp 145 150 155 160 Lys Asn
Asn Asn Asn Gln Asn Asn Gly Leu Leu Phe Ser Asp Glu Tyr 165 170 175
Leu Asn Leu Val Asp Tyr Asn Ser Ser Met Asp Tyr Lys Phe Thr Gly 180
185 190 Glu Tyr Ser Gln His Gln Gln Asn Cys Ser Val Pro Gln Thr Ser
Tyr 195 200 205 Gly Gly Asp Arg Val Val Pro Leu Lys Leu Glu Glu Ser
Arg Gly His 210 215 220 Gln Cys His Asn Gln Gln Asn Phe Gln Phe Asn
Ile Lys Tyr Gly Ser 225 230 235 240 Ser Gly Thr His Tyr Asn Asp Asn
Gly Ser Ile Asn His Asn Ala Tyr 245 250 255 Ile Ser Ser Met Glu Thr
Gly Val Val Pro Glu Ser Thr Ala Cys Val 260 265 270 Thr Thr Ala Ser
His Pro Arg Thr Pro Lys Gly Thr Val Glu Gln Gln 275 280 285 Pro Asp
Pro Ala Ser Gln Met Ile Thr Val Thr Gln Leu Ser Pro Met 290 295 300
Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Arg Lys Thr Arg 305
310 315 320 Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala Tyr
Ala Glu 325 330 335 Ile Arg Pro Arg Val Asn Gly Arg Phe Ala Lys Arg
Glu Ile Glu Ala 340 345 350 Glu Glu Gln Gly Phe Asn Thr Met Leu Met
Tyr Asn Thr Gly Tyr Gly 355 360 365 Ile Val Pro Ser Phe 370 (2)
INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 2493 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi)
ORIGINAL SOURCE: (A) ORGANISM: Zea mays (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 3: AAGCTTGGGC GTAGGTGTTG TCTATCGGCG AAAACACGCG
CGGTACGCCA AGAACAGCGC 60 GGCCATCTCC ATCCCAGGCA CGGTGCGCCC
GCTTTTTCGC CGTCTCGCTG AGTCACGGC 120 GGCGTCCAGC AGGTAGTTGA
GCGCCTTCCG CGGCACGAAT CGCTGCGTGC GGCCCGGAT 180 TGGTCGAGTT
GGTAGTCAGC GTCGGTGTCG AATGCCGGGA CGTCGACCAG GAAGAAGTT 240
CCGTCGCTGG GGTGGGGACG GAAGGCGTCA GGATTGTCGC AAGGGCAGAG CCCAGCCTG
300 GGGCGGGGCT ACCTCGTCGA CGCCTCGGCA CGGCGGCGGC AAAGCTGCTG
CGGGACGTG 360 CCGCCTGGGC CGCCTTCTCG GTGAAGTGGT CCTCGAAGGG
GACGAGCTCG CTGGGGTCA 420 ACCACCCCAT AGCTCGAGTC ACCGAAGAAG
GCGACGAGGA CGAGCCCGTC GCGGTGGCC 480 CGGTGTACCT CCTCGTCGTC
GGTGAGGCTG ACGCTGTAGA TATGGCCAGG CCACCACGG 540
TGGGACTTCA CCTTGGCCCA GACCATGTCG CCGAACCGGG GGCCGCCGTT CGCCCATGC
600 ATGCCGCGTC CGGCAGCAGG AACCATGGCG CCTCCAGCGG CGGGGTCGGA
CATCCTGTG 660 AGGGGAACCG AAAACCTAGA TTTGGATGCA GGTTCGATTG
GTCTGGGCTT GGGTTTGGG 720 TCCGGAGGAG GGTGGCCTGG GATCGGTGGA
AGGAGGGACA TTGTTGGTAA TTTTTATTA 780 TTTATAATAT GGAGAAATTC
GAGAGACTGA ACGATGGTGA TGTTTATTTG AGGACTATG 840 AGTATAAAGT
GTAAAATAGT ATTTTATCAA GTTTATATTC ACGTTTTTGC TGAAGATAG 900
ATAATAGTGG AGTTGTTTTT GGCGGCTACA TAATCTTAGG CTATCTTCTC GGTCGCTCT
960 ATATCATATC TACTATCACA TTCTCTATTT TAAATTTCAC TTTGTGTAAT CTACACTA
1020 AAATAGTGTT TTACACGGTA TGTTGTACAC AGCCTTATCG TGGCGCGACG
GAGTTGGA 1080 GAGATGGTGA ACAGCTGGAT AGATATGATT TATAGGCGAT
TGGGTAGATG TGATTTGA 1140 GGTGGTTATG TAGGAGCGAT TTAGTGAGAC
ATTGTAAATA ATTAGGTTGA TGTGATCC 1200 GGATGGCTAG GTAGATATGA
TTTTAATGGA TGGTTTGGTG GACTAAGTTA TGTGGACA 1260 ATAATATGTT
TTAAATTTCT AAGAAATTGT TTGTGTTAAA TTGTATCCCA CATAGATT 1320
TTAGCCATCT CAAAGAGAGG TTTGGGTTGT TTACACAAAT AAAATATTCG TTTGCTTC
1380 CAATTTATAT GTTTTTTATT TACATGAAAA CTATATTTTT TATTCATCTA
CTCACCCA 1440 ACAGAAATTC TGGTTGAGTA GATGAAAAAA AACTACAACA
AACTCTTCCT GAAAGTGT 1500 GTGTGAAGCC GAGAAATCCT TTTCATTTCG
GTGACGGAGC CCCTTGCTGG CTGCTGCT 1560 GTGCACTCCG TTCGCCTGCC
TGCCACTACA AGCGACGGCC GACGACTCGC AAGTATCG 1620 AGGCATTTTA
AAACTGAAAA CCAAATCTAA ACCCGAATAG ACCAAATTGT TGGTTTAT 1680
GGGTTTTTGG GTTCGGATTC GGTTTCTAAA TATGCTATAT TTTAGGGTAT AGGTTCGG
1740 TCAGTTTCTA ACCTTTAAAA CCTGAATAGA CGAATAACCC GAAATATAAA
AAATCTCT 1800 ATATGTGATG ATATTATTAT ATGATTTATG AACTTATTAA
CCGAAAATAA TGATACCA 1860 CTAACGATAG TATATATATC TATGTATGCT
ATTTTTATAG TCACTTGTTG TAATAATA 1920 ACTTCCAATT AATTAATCAG
TGTATATATT TTAACAAAAG ATACTAGCCT CTCTACTA 1980 TGAGTATATT
CGGTGCACCG AATAGACCGA ACCGAAATTG TAAGTCTATT CAGGTTCG 2040
TCCTAAAATT ATTTTAAAAA TTTTGGTTCT CATATTTCAG AATCCGAAAT TTCATAAA
2100 CAAATAGACC GAACCAAATT ACGCTAATAG ACCGAATAAC TAGCGTACTC
GCAAGTCG 2160 CCCCACTAGC CTGCTGCGTG CGTAAGCGAG GACGTCACGC
GTTCTCCCTC CCGTCGAC 2220 AATACACTTG GTCTTCTAGC ACCTTCTTCC
TCTCCAAGAC TCCAATCCCC CAACCACC 2280 AACCAGCGCC AGCTCTAACG
TCACCTCTGA TTTCTCTCTC CTCTCTATTG CTAGCTGC 2340 TATTATAAGT
AGCAGCTGCA GCAGGCAGGA GCTGCACACA CCCATCCAAT TCCAGCTG 2400
GATCTTGATC CTGCACCCCG AGCCGTACAC AAGAGCTAGT CGGTAGAACT TGCAGGAG
2460 GAGCAGAACT AAGTGCAGAG AACAGGACAT ATG 2493 (2) INFORMATION FOR
SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4201 base
pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY:
linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A)
ORGANISM: Arabidopsis thaliana (B) STRAIN: Landsberg erecta (viii)
POSITION IN GENOME: (B) MAP POSITION: chromosome 5 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 4: GAATTCATGT ACCAAATCAA TACTTTTTAG
CCATAAATGA GTCAGTTTTA GTATCCACAT 60 GAATTTACCT ACCAGAGTGT
TGTAAATTAT GTTCTTTTGG GGCCACTTAC ATGGATCTC 120 TTCATTCACT
GCAGCGAGTT CTCAGACCAC CAGAAAATTT ATTCAGTGAT CTGTTTTGA 180
CATGCAACAT AAACTTATAA GCCACACAAG CAAAACAAAG ATATCCCATG TTGCATATA
240 TACGAGCTAG CATATCATAA AGAAGGAAAC TTGAAGTAGC AAAGTTTCTA
CTAAATTTC 300 TGTCAGGAAT TTTTAAAATG CAATGACAAC CACTTGGAGC
ACTATGAGTT TCAGAGCCA 360 TAGAATGTTA CTATTTGGTG TGGATTCGAG
CTAGCACGTG AAAGTGCATA AAAGTGATT 420 CCTTTTGCCA AAGGTCACTG
CACTTTTCCT CAGATAGTTT CTCACAGCCA TGGAAAGTG 480 AGAATCCGCA
TAAACGTACA ATTACAAGCT TTATATGGTC CCTCGACTCT TATTCTCTT 540
TCAGTCTTTG CAACTAAATA GGGTTTTCGT TAATCTGAAA GAAGCAAAGT ATTCGAAAC
600 ACGGAAACCT GATAAAGAAT GAAAACAAAT AAGCAATAGT GTTTTCTTGA
AAATCTCGA 660 GCAACTTTGA GGATATTGTT ACATATGATC TATTACTCGT
AACAGTTATC CGAAGGCCT 720 CACATGTGAG AGAAGTTCCA AACCGCTACA
ACAATAAACT TAATTAGAGA CTGTCAACG 780 GCAATAATAA GCAAAACTAC
TTTTTTCTTG AGCTACAAGT GAAAAGGCCA ATACACAAT 840 TACTCTTCAT
GAACTCGAAC CACGTTACAA TCTCCAAAAA ATTTCATCAC CAAAGCACT 900
AAAGCCAAAG ATGCCTCAAC TTATCCAACT TGGCAGGATA AAGATCTCCA AAAATGCTT
960 CTAAAGAACC TAGAATCTTT TCTTTAGAAT TCAATGATCA TATAACCATT TCATAACA
1020 TCTAAATGCC ATTACATTCA TCGTAAAACC AGTAAATAAC AAGAACTTGT
ATGTTAAG 1080 CCAATTACCA AGCAAAAAAA AACTTTTCAA AGTTTAAAGT
TCAAAATGGG AAAGAGAA 1140 GCGGTGTAAG CAAATATGAA AGAGGAAGAG
ATGCGAAAAG TGTATCCTAG GACCAGCA 1200 TTATACAAAA AAAAAACACT
CACTTTTCAG CTCTTAAGGC ATAGAGTGAA GGTAGCCA 1260 TGAATTTGGC
CACTAGAGCG TCCGTCAAAT CTCATTCTTT TTGGACCACA TAATGGGT 1320
CATACATTCA CTGGACCCAA AAGCGTAACT GGAGCTAGTC CTCAAACCTA GAGAGTAT
1380 TATCCTGTAG CTTCCACATA GTAAACATTA TGAGCATAAC ACCAACAAGG
CAACTCCA 1440 TACTAATGGT TATTAGTACA GGAAAACCCA CGATGCTAAA
CACATGAATG GGTCACCA 1500 TAGAGTGAAG ATGGTTAAAT TGCATCTATG
GATCATGTGG ACTAGTAAAT GAGTGTAG 1560 GAAAACTTCA CAATTACCTC
TGTGATCTTA GAAACATGTC CTGAAAATTC CATACAAG 1620 TCGTTTGTAT
TAGATTACTT CCACAGGTTG AGATCTAATA AAGCTACAAT AAATAGTA 1680
GAGTATCATC ATAAACCCAA ATTACAGAGA TGTGACAACA CTCATGAGTC ATGTTTTG
1740 ACTACTTACT ATAATGGTTA CCAAGTGCAA ATTTCTACAT ACTATATATG
ATAAATCT 1800 TTATTGCTCA TGTGGACTCC AAAATGCCTT TTAAGTTTTA
ACTTGTGCGT CAGGTAAA 1860 CTAATTTGTA GTCTCAAGAC TACTTGGCGG
ATTCGAGTTT GATCCTAGAA AATCCACC 1920 CTCTATGTTT TTCATGTCAC
TTTTCCGATA TGATTCTCAT TACCATGACT TTATGAAC 1980 GATTAAACAT
TATAACACTT TTCATCAGAA AATCCTTCGA AAGTTTCAAT TGCAAATC 2040
TCTAAATGAT GCAGATGCAT TCACAAATAA TGGAACAACA ACTATACCAT ATTCACGA
2100 TTGTCTAACC TTTGTATAGG TAGTCAACCC ATAACAGTTG GTGATGGCTC
TGACACTC 2160 AGCCTTACTC GGAGAGATAC CTGAACAGTA ATCACAAGGT
TCAGGATGAA TATTCAAC 2220 CTTAAACTTT GTATAAAGCC AAAGAGATAA
AACGAATCTA GCTTTACTTT AAATAAAA 2280 CATATGAAAA TAGTAAAAGG
TGATACGAAA AAATAGTAAC AATTTGCCTG CAACACCA 2340 GCATTATCCG
GACCACTTCC TCTTGAGAAT CTCAGTATGG CAAGTGGCAA AACCTAAG 2400
ACTTGTGAAC GGGTCCCAAC GAAGAAGTGC ATAGGAGGAG ATGTTTACAC TTTACACT
2460 ACACTTTACA CTTTACACAT AGGCCTTCCC AAAAGCTCAA CTAGCTGCAA
GAGGATCC 2520 TAACATGTAA GAGCCACTAA CGCTGTGCCA CGTGTAGGCA
CTCAGGATTC GATCTTCC 2580 TCTACTTATT CTCTCACACC AGATATAAGC
TTTATTAGCC CCTTCTTTCA GATACCAG 2640 CCCACACCAT CAAACTTACT
ACATCTGAGT TATTATGTTG AAACAAGAGA GTAACGAC 2700 AGGTAGTGGA
GAGAACAACA GGGCACGACC CTGTGACACA TGCCGGTCAA ACGCCTGC 2760
CGTGTATTGC CATGCAGATT CTGCCTACTT GTGCATGAGC TGTGATGCTC AAGTTCAC
2820 TGCCAATCGC GTTGCTTCCC GCCATAAACG TGTCCGGGTC TGCGAGTCAT
GTGAGCGT 2880 TCCGGCTGCT TTTTTGTGTG AGGCAGATGA TGCCTCTCTA
TGCACAGCCT GTGATTCA 2940 GGTTCATTCT GCAAACCCAC TTGCTAGACG
CCATCAGCGA GTTCCAATTC TACCAATT 3000 TGGAAACTCT TTCAGCTCCA
TGACCACTAC TCACCACCAA AGCGAGAAAA CAATGACC 3060 TCCAGAGAAG
AGACTGGTGG TGGATCAAGA GGAAGGTGAA GAAGGTGATA AGGATGCC 3120
GGAGGTTGCT TCGTGGCTGT TCCCTAATTC AGACAAAAAT AACAATAACC AAAACAAT
3180 GTTATTGTTT AGTGATGAGT ATCTAAACCT TGTGGATTAC AACTCGAGTA
TGGACTAC 3240 ATTCACAGGT GAATACAGTC AACACCAACA AAACTGCAGC
GTACCACAGA CGAGCTAC 3300 GGGAGATAGA GTTGTTCCGC TTAAACTTGA
AGAATCAAGG GGCCACCAGT GCCATAAC 3360 ACAGAATTTT CAGTTCAATA
TCAAATATGG CTCCTCAGGG ACTCACTACA ACGACAAT 3420 TTCCATTAAC
CATAACGTAA GGCTTTTGTA TATTTGTTAC CCCTTCAATT TAGCATCT 3480
CCATAACGCA GCAGGGTGAA TTCTTTCATC ATACACACAA ATCCACTGAT CCACTGCC
3540 CAGTTGATCT ATAGCACATA GAAATTTCAC CAGAAGTCTA TAATAAAAAC
AATATATG 3600 TCCTTTTGCA TCGACTCTCT TTAGTCCTCT TACCAGGGGG
ATTGAGAATG TCTTTGTT 3660 TGTCATTAGG CATACATTTC ATCCATGGAA
ACTGGTGTTG TGCCGGAGTC AACAGCAT 3720 GTCACAACAG CTTCACACCC
AAGAACGCCC AAAGGGACAG TAGAGCAACA ACCTGACC 3780 GCAAGCCAGA
TGATAACAGT AACACAACTC AGTCCAATGG ACAGAGAAGC CAGGGTCC 3840
AGATACAGAG AGAAGAGGAA GACAAGGAAA TTTGAGAAGA CAATAAGGTA TGCTTCGA
3900 AAGGCATATG CAGAGATAAG ACCGCGGGTC AATGGCCGGT TCGCAAAGAG
AGAAATCG 3960 GCCGAGGAGC AAGGGTTCAA CACGATGCTA ATGTACAACA
CAGGATATGG GATTGTTC 4020 TCATTCTGAT ACTCCTGTGG CAAAAAGAAA
AACTAGATTG CAAGCTGTAA ATTACTTT 4080 GTTTGAGATT ATGTTAGGTT
TGGTGAAATT CTTAGCTTCA AGAAGTATTA CTACTGTT 4140 GCAAATGGGT
TTGTAGTTTT GGCTAATTAA AACTATAGTA TTCTTCTTTC TCTGCATT 4200 T 4201
(2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 1101 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii)
HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Brassic napus
(ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1098 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 5: ATG TTC AAA CAA GAG AGT AAC AAC ATT GGT
AGT GAA GAG AAC AAC ACC 48 Met Phe Lys Gln Glu Ser Asn Asn Ile Gly
Ser Glu Glu Asn Asn Thr 375 380 385
GGG GCG CGA GCT TGT GAC ACA TGC GGG TCA ACC ATC TGC ACC GTG TAC 96
Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr Ile Cys Thr Val Tyr 390
395 400 405 TGC CAT GCT GAC TCC GCC TAC TTA TGC AAT AGC TGC GAT GCT
CAA GTC 144 Cys His Ala Asp Ser Ala Tyr Leu Cys Asn Ser Cys Asp Ala
Gln Val 410 415 420 CAC TCT GCC AAT CGC GTT GCT TCC CGC CAT AAA AGG
GTC AGA GTG TGC 192 His Ser Ala Asn Arg Val Ala Ser Arg His Lys Arg
Val Arg Val Cys 425 430 435 GAG TCA TGT GAG CGT GCC CCT GCT GCT TTT
ATG TGT GAG GCA GAT GAT 240 Glu Ser Cys Glu Arg Ala Pro Ala Ala Phe
Met Cys Glu Ala Asp Asp 440 445 450 GTG TCT CTA TGC ACA GCC TGT GAT
TCA GAG GTT CAC TCC GCA AAC CCT 288 Val Ser Leu Cys Thr Ala Cys Asp
Ser Glu Val His Ser Ala Asn Pro 455 460 465 CTT GCT AGA CGC CAT CAG
CGA GTT CCA GTT GTG CCG ATA ACT GGA AAC 336 Leu Ala Arg Arg His Gln
Arg Val Pro Val Val Pro Ile Thr Gly Asn 470 475 480 485 TCT TGC AGC
TCC TTG GCC ACC GCT AAC CAC ACA ACA GTG ACC GAG CCA 384 Ser Cys Ser
Ser Leu Ala Thr Ala Asn His Thr Thr Val Thr Glu Pro 490 495 500 GAG
AAG AGA GTG GTG TTA GTT CAA GAG GAT GCC AAA GAG ACG GCT TCA 432 Glu
Lys Arg Val Val Leu Val Gln Glu Asp Ala Lys Glu Thr Ala Ser 505 510
515 TGG TTG TTC CCT AAA AAC AGT GAC AAT CAC AAC AAC AAC AAC CAG AAC
480 Trp Leu Phe Pro Lys Asn Ser Asp Asn His Asn Asn Asn Asn Gln Asn
520 525 530 AAT GAG TTG TTG TTT AGT GAT GAC TAT CTA GAC CTT GCT GAT
TAC AAC 528 Asn Glu Leu Leu Phe Ser Asp Asp Tyr Leu Asp Leu Ala Asp
Tyr Asn 535 540 545 TCG AGT ATG GAC TAC AAG TTC ACT GGT CAA TAC AAT
CAA CCT ACT CAA 576 Ser Ser Met Asp Tyr Lys Phe Thr Gly Gln Tyr Asn
Gln Pro Thr Gln 550 555 560 565 CAT AAA CAA GAC TGC ACC GTA CCA GAG
AAA AAC TAC GGT GGA GAT AGA 624 His Lys Gln Asp Cys Thr Val Pro Glu
Lys Asn Tyr Gly Gly Asp Arg 570 575 580 GTT GTT CCA CTC CAA CTT GAA
GAA ACA AGA GGA AAC TTG CAC CAC AAG 672 Val Val Pro Leu Gln Leu Glu
Glu Thr Arg Gly Asn Leu His His Lys 585 590 595 CAA CAT AAT ATC ACG
TAT GGC TCC TCA GGA AGT CAC TAC AAC AAC AAT 720 Gln His Asn Ile Thr
Tyr Gly Ser Ser Gly Ser His Tyr Asn Asn Asn 600 605 610 GGT TCC ATA
AAC CAT AAC GCA TAC AAT CCA TCA ATG GAA ACT GAC TTT 768 Gly Ser Ile
Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp Phe 615 620 625 GTT
CCG GAG CAG ACA GCA CCT GAC AAA ACA GTT TCA CAT CCA AAA ACG 816 Val
Pro Glu Gln Thr Ala Pro Asp Lys Thr Val Ser His Pro Lys Thr 630 635
640 645 CAC AAA GGG AAG ATA GAG AAA CTA CCT GAA CCT CTA ATT CAG ATT
CTC 864 His Lys Gly Lys Ile Glu Lys Leu Pro Glu Pro Leu Ile Gln Ile
Leu 650 655 660 AGT CCA ATG GAC AGA GAA GCT AGA GTC CTG AGA TAC AGA
GAG AAG AAG 912 Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg
Glu Lys Lys 665 670 675 AAG AGA AGA AAG TTT GAG AAG ACA ATA AGG TAT
GCT TCA AGG AAG GCA 960 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg Tyr
Ala Ser Arg Lys Ala 680 685 690 TAT GCA GAG AGA AGA CCG AGG ATC AAT
GGA CGG TTT GCA AAG ATT AGT 1008 Tyr Ala Glu Arg Arg Pro Arg Ile
Asn Gly Arg Phe Ala Lys Ile Ser 695 700 705 GAA ACC GAA GTA GAG GAC
CAA GAG TAC AAC ACA ATG CTA ATG TAC TAT 1056 Glu Thr Glu Val Glu
Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr Tyr 710 715 720 725 GAC ACA
GGA TAT GGC ATT GTT CCT TCA TTC TAT GGC CAA AAA 1098 Asp Thr Gly
Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys 730 735 TAA 1101 (2)
INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 366 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
6: Met Phe Lys Gln Glu Ser Asn Asn Ile Gly Ser Glu Glu Asn Asn Thr
1 5 10 15 Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr Ile Cys Thr
Val Tyr 20 25 30 Cys His Ala Asp Ser Ala Tyr Leu Cys Asn Ser Cys
Asp Ala Gln Val 35 40 45 His Ser Ala Asn Arg Val Ala Ser Arg His
Lys Arg Val Arg Val Cys 50 55 60 Glu Ser Cys Glu Arg Ala Pro Ala
Ala Phe Met Cys Glu Ala Asp Asp 65 70 75 80 Val Ser Leu Cys Thr Ala
Cys Asp Ser Glu Val His Ser Ala Asn Pro 85 90 95 Leu Ala Arg Arg
His Gln Arg Val Pro Val Val Pro Ile Thr Gly Asn 100 105 110 Ser Cys
Ser Ser Leu Ala Thr Ala Asn His Thr Thr Val Thr Glu Pro 115 120 125
Glu Lys Arg Val Val Leu Val Gln Glu Asp Ala Lys Glu Thr Ala Ser 130
135 140 Trp Leu Phe Pro Lys Asn Ser Asp Asn His Asn Asn Asn Asn Gln
Asn 145 150 155 160 Asn Glu Leu Leu Phe Ser Asp Asp Tyr Leu Asp Leu
Ala Asp Tyr Asn 165 170 175 Ser Ser Met Asp Tyr Lys Phe Thr Gly Gln
Tyr Asn Gln Pro Thr Gln 180 185 190 His Lys Gln Asp Cys Thr Val Pro
Glu Lys Asn Tyr Gly Gly Asp Arg 195 200 205 Val Val Pro Leu Gln Leu
Glu Glu Thr Arg Gly Asn Leu His His Lys 210 215 220 Gln His Asn Ile
Thr Tyr Gly Ser Ser Gly Ser His Tyr Asn Asn Asn 225 230 235 240 Gly
Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp Phe 245 250
255 Val Pro Glu Gln Thr Ala Pro Asp Lys Thr Val Ser His Pro Lys Thr
260 265 270 His Lys Gly Lys Ile Glu Lys Leu Pro Glu Pro Leu Ile Gln
Ile Leu 275 280 285 Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr
Arg Glu Lys Lys 290 295 300 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg
Tyr Ala Ser Arg Lys Ala 305 310 315 320 Tyr Ala Glu Arg Arg Pro Arg
Ile Asn Gly Arg Phe Ala Lys Ile Ser 325 330 335 Glu Thr Glu Val Glu
Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr Tyr 340 345 350 Asp Thr Gly
Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys 355 360 365 NFORMATION
FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1101
base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D)
TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii)
HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Brassica
napus (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1098 (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 7: ATG TTC AAA CAA GAG AGT AAC AAC
ATT TGT AAT AGA GAG AAC AAC AGA 48 Met Phe Lys Gln Glu Ser Asn Asn
Ile Cys Asn Arg Glu Asn Asn Arg 370 375 380 GGG GCA CGA GCC TGT GAC
ACA TGC GGG TCA ACC ATC TGC ACC GTG TAC 96 Gly Ala Arg Ala Cys Asp
Thr Cys Gly Ser Thr Ile Cys Thr Val Tyr 385 390 395 TGC CAT GCT GAC
TCT GCC TAC TTA TGC AAT AGC TGC GAT GCT CAA GTC 144 Cys His Ala Asp
Ser Ala Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 400 405 410 CAC TCT
GCC AAT CGC GTT GCT TCC CGC CAT AAA CGT GTC CGG GTC TGC 192 His Ser
Ala Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 415 420 425
430 GAG TCA TGT GAG CGT GCC CCT GCT GCT TTT ATG TGT GAG GCA GAT GAT
240 Glu Ser Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp
435 440 445 GTG TCT CTA TGC ACA GCC TGT GAT TTA GAG GTT CAC TCC GCA
AAC CCT 288 Val Ser Leu Cys Thr Ala Cys Asp Leu Glu Val His Ser Ala
Asn Pro 450 455 460 CTT GCT AGA CGC CAT CAG CGA GTT CCA GTT GTG CCG
ATA ATT GGA AAC 336 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro
Ile Ile Gly Asn 465 470 475 TCT TGC AGC TCC TTG GCC ACC GCT AAC CAC
ACA ACA GTG ACC GAG CCA 384 Ser Cys Ser Ser Leu Ala Thr Ala Asn His
Thr Thr Val Thr Glu Pro 480 485 490 GAG AAG AGA GTG GTG TTA GTT CAA
GAG GAT GCC AAA GAG ACG GCT TCA 432 Glu Lys Arg Val Val Leu Val Gln
Glu Asp Ala Lys Glu Thr Ala Ser 495 500 505 510 TGG TTG TTC CCT AAA
AAC AGT GAC TAT CAC AAC AAC AAC AAC AAC CAG 480 Trp Leu Phe Pro Lys
Asn Ser Asp Tyr His Asn Asn Asn Asn Asn Gln 515 520 525 AAC AAT GAG
TTG TTG TTT AGT GAT GAC TAC CTA GAC CTT GCT GAT TAC 528 Asn Asn Glu
Leu Leu Phe Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr 530 535 540 AAC
TCC AGT ATG GAC TAC AAG TTC ACC AGT CAA TAC AAT CAA CCT CGA 576 Asn
Ser Ser Met Asp Tyr Lys Phe Thr Ser Gln Tyr Asn Gln Pro Arg 545 550
555 CAT AAA CAA GAC TGC ATC GTA CCA GAG AAA AAC TAC AGT GGA GAT AGA
624 His Lys Gln Asp Cys Ile Val Pro Glu Lys Asn Tyr Ser Gly Asp Arg
560 565 570 GTT GTT CCG CTC CAA CTT GAA GAA ACA AGA GGA AAC TTG CGG
AAC AAG 672 Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu Arg
Asn Lys 575 580 585 590 CAA CAG AAT ATC ACA TAT GGC TCC TCA GGA AGC
CAA TAC AAC AAC AAC 720
Gln Gln Asn Ile Thr Tyr Gly Ser Ser Gly Ser Gln Tyr Asn Asn Asn 595
600 605 GGT TCC ATT AAC CAT AAC GCA TAC AAT CCA TCA ATG GAA ACT GAC
TTT 768 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp
Phe 610 615 620 GTG CCG GAG CAG ACA GCA CCT GAC ACA ACA GTT TCA CAT
CCA AAA ACG 816 Val Pro Glu Gln Thr Ala Pro Asp Thr Thr Val Ser His
Pro Lys Thr 625 630 635 CAC AAA GGG AAG ACA GCA CAA CTA CCT GAA CCT
CTA ATT CAG ATT CTC 864 His Lys Gly Lys Thr Ala Gln Leu Pro Glu Pro
Leu Ile Gln Ile Leu 640 645 650 AGT CCA ATG GAC AGA GAA GCT AGA GTC
CTG AGA TAC AGA GAG AAG AAG 912 Ser Pro Met Asp Arg Glu Ala Arg Val
Leu Arg Tyr Arg Glu Lys Lys 655 660 665 670 AAG AGA AGA AAG TTT GAG
AAG ACA ATA AGG TAT GCT TCA AGG AAG GCA 960 Lys Arg Arg Lys Phe Glu
Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 675 680 685 TAT GCA GAG AGA
AGA CCG AGG ATA AAT GGA CGG TTT GCA AAG ATG AGT 1008 Tyr Ala Glu
Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Met Ser 690 695 700 GAA
ACC GAA GTA GAG GAC CAA GAG TAC AAC ACA ATG CTA ATG TAC TGC 1056
Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr Cys 705
710 715 GAC ACA GGA TAT GGC ATT GTT CCT TCA TTC TAT GGC CAA AAA
1098 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys 720
725 730 TAA 1101 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 366 amino acids (B) TYPE: amino acid
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 8: Met Phe Lys Gln Glu Ser Asn Asn Ile Cys
Asn Arg Glu Asn Asn Arg 1 5 10 15 Gly Ala Arg Ala Cys Asp Thr Cys
Gly Ser Thr Ile Cys Thr Val Tyr 20 25 30 Cys His Ala Asp Ser Ala
Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 35 40 45 His Ser Ala Asn
Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 50 55 60 Glu Ser
Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 65 70 75 80
Val Ser Leu Cys Thr Ala Cys Asp Leu Glu Val His Ser Ala Asn Pro 85
90 95 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro Ile Ile Gly
Asn 100 105 110 Ser Cys Ser Ser Leu Ala Thr Ala Asn His Thr Thr Val
Thr Glu Pro 115 120 125 Glu Lys Arg Val Val Leu Val Gln Glu Asp Ala
Lys Glu Thr Ala Ser 130 135 140 Trp Leu Phe Pro Lys Asn Ser Asp Tyr
His Asn Asn Asn Asn Asn Gln 145 150 155 160 Asn Asn Glu Leu Leu Phe
Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr 165 170 175 Asn Ser Ser Met
Asp Tyr Lys Phe Thr Ser Gln Tyr Asn Gln Pro Arg 180 185 190 His Lys
Gln Asp Cys Ile Val Pro Glu Lys Asn Tyr Ser Gly Asp Arg 195 200 205
Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu Arg Asn Lys 210
215 220 Gln Gln Asn Ile Thr Tyr Gly Ser Ser Gly Ser Gln Tyr Asn Asn
Asn 225 230 235 240 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met
Glu Thr Asp Phe 245 250 255 Val Pro Glu Gln Thr Ala Pro Asp Thr Thr
Val Ser His Pro Lys Thr 260 265 270 His Lys Gly Lys Thr Ala Gln Leu
Pro Glu Pro Leu Ile Gln Ile Leu 275 280 285 Ser Pro Met Asp Arg Glu
Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys 290 295 300 Lys Arg Arg Lys
Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 305 310 315 320 Tyr
Ala Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Met Ser 325 330
335 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr Cys
340 345 350 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys
355 360 365 (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
other nucleic acid (A) DESCRIPTION: /desc = "primer used for PCR"
(vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 9: TCCTGCTCGC TTCGCTACTT 20 (2) INFORMATION
FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20
base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D)
TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A)
DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL SOURCE:
(A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:
GCGATGCTGT CGGAATGGAC 20 (2) INFORMATION FOR SEQ ID NO: 11: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 11: AAGTACTCTC GGTAGCCAAG 20 (2)
INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for pcr" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
12: GTGTGGTCGC CATGATCGCG 20 (2) INFORMATION FOR SEQ ID NO: 13: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 13: CTGGGAAGTG AATGGAGACA TA 22
(2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
14: AGGAGTCGCA TAAGGGAGAG 20 (2) INFORMATION FOR SEQ ID NO: 15: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 15: ACGTCGGATG CTCACTATAG GGATC
25
(2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
16: GTGATAAACT ACCGCATTAA AGC 23 (2) INFORMATION FOR SEQ ID NO: 17:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 17: ACGTCGGATG ACTTTAATTT ATTCACTA
28 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
18: GACGTGGATG CTCACTAAAG GGATC 25 (2) INFORMATION FOR SEQ ID NO:
19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: AGAGCCTTCA ACCCAGTCAG 20
(2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
20: ACGTCGGATG CCGATCTCAA GATTA 25 (2) INFORMATION FOR SEQ ID NO:
21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
GCTCCCACAC CATCAAACTT ACTAC 25 (2) INFORMATION FOR SEQ ID NO: 22:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis
thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: CTCCTCGGCT
TCGATTTCTC 20 (2) INFORMATION FOR SEQ ID NO: 23: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
other nucleic acid (A) DESCRIPTION: /desc = "primer used for PCR"
(vi) ORIGINAL SOURCE: (A) ORGANISM: designed primer not derived
from any organism (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:
GACTCGAGTC GACATCG 17 (2) INFORMATION FOR SEQ ID NO: 24: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
TGCAGATTCT GCCTACTTGT GC 22 (2) INFORMATION FOR SEQ ID NO: 25: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
TGATTCTGCC TACTTGTGCT C 21 (2) INFORMATION FOR SEQ ID NO: 26: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
synthesised for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:
GCTTGGTTTG CCTCTTCATC 20 (2) INFORMATION FOR SEQ ID NO: 27: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
synthesised for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
GGTCCCAACG AAGAAGTGC 19 (2) INFORMATION FOR SEQ ID NO: 28: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
CAGGGAGGCG TGAAAGTGT 19 (2) INFORMATION FOR SEQ ID NO: 29: (i)
SEQUENCE CHARACTERISTICS:
(A) LENGTH: 10 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "adapter synthesised to (vi) ORIGINAL
SOURCE: (A) ORGANISM: designed sequence not derived from any
organism (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29: TACAAGCTTG 10
(2) INFORMATION FOR SEQ ID NO: 30: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "oligonucleotide (vi) ORIGINAL SOURCE: (A)
ORGANISM: designed sequence, not derived from any organism (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 30: TATCGATAGT AC 12 (2)
INFORMATION FOR SEQ ID NO: 31: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
31: TTCAAGCTCT ACGCCGGA 18 (2) INFORMATION FOR SEQ ID NO: 32: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 amino acids (B) TYPE:
amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (ix)
FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER
INFORMATION: /label= Xaa /note= Xaa at positions 2, 3, 5-20, 22 and
23 can be any amino acid (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:
Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5
10 15 Xaa Xaa Xaa Xaa Cys Xaa Xaa Cys 20 (2) INFORMATION FOR SEQ ID
NO: 33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 amino acids
(B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
protein (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1
(D) OTHER INFORMATION: /label= Xaa /note= Xaa at positions 2, 3,
5-21, 23 and 24 can be any amino acid (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 33: Cys Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Cys 20 25
SEQUENCE LISTING (1) GENERAL INFORMATION: 1 (i) APPLICANT:
Coupland, George M. Putterill, Joanna J. (ii) TITLE OF INVENTION:
Genetic control of flowering (iii) NUMBER OF SEQUENCES: 33 (iv)
CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Nixon & Vanderhye PC (B)
STREET: 8th Floor, 1100 North Glebe Road 81100 (C) CITY: Arlington
(D) STATE: Virginia (E) COUNTRY: United States of America (F) ZIP:
22201-4714 222014714 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE:
Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM:
PC-DOS/MS-DOS (D) SOFTWARE: PatentIn Release #1.0, Version #1.25
(EPO) 10125 (vi) CURRENT APPLICATION DATA: (A) APPLICATION
NUMBER:US/11/314,171 (B) FILING DATE: 22-DEC-2005 222005 (C)
CLASSIFICATION: (vii) PRIOR APPLICATION DATA: (A) APPLICATION
NUMBER: US/10/143,849 10143849 (B) FILING DATE: 14-May-2002 142002
(C) CLASSIFICATION: (vii) PRIOR APPLICATION DATA: (A) APPLICATION
NUMBER: US 09/516,191 09516191 (B) FILING DATE: 01-MAR-2000 012000
(A) APPLICATION NUMBER: US 08/945,056 08945056 (B) FILING DATE:
20-OCT-1997 201997 (A) APPLICATION NUMBER: PCT/GB95/02561 9502561
(B) FILING DATE: 01-NOV-1995 011995 (A) APPLICATION NUMBER: GB
9422083.7 94220837 (B) FILING DATE: 02-NOV-1994 021994 (viii)
ATTORNEY/AGENT INFORMATION: (A) NAME: Ms Mary J Wilson (B)
REGISTRATION NUMBER: 32,955 32955 (C) REFERENCE/DOCKET NUMBER:
620-197 620197 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE:
(703) 816-4000 7038164000 (B) TELEFAX: (703) 816-4100 7038164100
(2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 1124 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA to mRNA (iii)
HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A)
ORGANISM: Arabidopsis thaliana (B) STRAIN: Landsberg erecta (viii)
POSITION IN GENOME: (A) CHROMOSOME/SEGMENT: chromosome 5 (ix)
FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1119 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 1: ATG TTG AAA CAA GAG AGT AAC GAC ATA GGT
AGT GGA GAG AAC AAC AGG 48 Met Leu Lys Gln Glu Ser Asn Asp Ile Gly
Ser Gly Glu Asn Asn Arg 1 5 10 15 GCA CGA CCC TGT GAC ACA TGC CGG
TCA AAC GCC TGC ACC GTG TAT TGC 96 Ala Arg Pro Cys Asp Thr Cys Arg
Ser Asn Ala Cys Thr Val Tyr Cys 20 25 30 CAT GCA GAT TCT GCC TAC
TTG TGC ATG AGC TGT GAT GCT CAA GTT CAC 144 His Ala Asp Ser Ala Tyr
Leu Cys Met Ser Cys Asp Ala Gln Val His 35 40 45 TCT GCC AAT CGC
GTT GCT TCC CGC CAT AAA CGT GTC CGG GTC TGC GAG 192 Ser Ala Asn Arg
Val Ala Ser Arg His Lys Arg Val Arg Val Cys Glu 50 55 60 TCA TGT
GAG CGT GCT CCG GCT GCT TTT TTG TGT GAG GCA GAT GAT GCC 240 Ser Cys
Glu Arg Ala Pro Ala Ala Phe Leu Cys Glu Ala Asp Asp Ala 65 70 75 80
TCT CTA TGC ACA GCC TGT GAT TCA GAG GTT CAT TCT GCA AAC CCA CTT 288
Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala Asn Pro Leu 85
90 95 GCT AGA CGC CAT CAG CGA GTT CCA ATT CTA CCA ATT TCT GGA AAC
TCT 336 Ala Arg Arg His Gln Arg Val Pro Ile Leu Pro Ile Ser Gly Asn
Ser 100 105 110 TTC AGC TCC ATG ACC ACT ACT CAC CAC CAA AGC GAG AAA
ACA ATG ACC 384 Phe Ser Ser Met Thr Thr Thr His His Gln Ser Glu Lys
Thr Met Thr 115 120 125 GAT CCA GAG AAG AGA CTG GTG GTG GAT CAA GAG
GAA GGT GAA GAA GGT 432 Asp Pro Glu Lys Arg Leu Val Val Asp Gln Glu
Glu Gly Glu Glu Gly 130 135 140 GAT AAG GAT GCC AAG GAG GTT GCT TCG
TGG CTG TTC CCT AAT TCA GAC 480 Asp Lys Asp Ala Lys Glu Val Ala Ser
Trp Leu Phe Pro Asn Ser Asp 145 150 155 160 AAA AAT AAC AAT AAC CAA
AAC AAT GGG TTA TTG TTT AGT GAT GAG TAT 528 Lys Asn Asn Asn Asn Gln
Asn Asn Gly Leu Leu Phe Ser Asp Glu Tyr 165 170 175 CTA AAC CTT GTG
GAT TAC AAC TCG AGT ATG GAC TAC AAA TTC ACA GGT 576 Leu Asn Leu Val
Asp Tyr Asn Ser Ser Met Asp Tyr Lys Phe Thr Gly 180 185 190 GAA TAC
AGT CAA CAC CAA CAA AAC TGC AGC GTA CCA CAG ACG AGC TAC 624 Glu Tyr
Ser Gln His Gln Gln Asn Cys Ser Val Pro Gln Thr Ser Tyr 195 200 205
GGG GGA GAT AGA GTT GTT CCG CTT AAA CTT GAA GAA TCA AGG GGC CAC 672
Gly Gly Asp Arg Val Val Pro Leu Lys Leu Glu Glu Ser Arg Gly His 210
215 220 CAG TGC CAT AAC CAA CAG AAT TTT CAG TTC AAT ATC AAA TAT GGC
TCC 720 Gln Cys His Asn Gln Gln Asn Phe Gln Phe Asn Ile Lys Tyr Gly
Ser 225 230 235 240 TCA GGG ACT CAC TAC AAC GAC AAT GGT TCC ATT AAC
CAT AAC GCA TAC 768 Ser Gly Thr His Tyr Asn Asp Asn Gly Ser Ile Asn
His Asn Ala Tyr 245 250 255
ATT TCA TCC ATG GAA ACT GGT GTT GTG CCG GAG TCA ACA GCA TGT GTC 816
Ile Ser Ser Met Glu Thr Gly Val Val Pro Glu Ser Thr Ala Cys Val 260
265 270 ACA ACA GCT TCA CAC CCA AGA ACG CCC AAA GGG ACA GTA GAG CAA
CAA 864 Thr Thr Ala Ser His Pro Arg Thr Pro Lys Gly Thr Val Glu Gln
Gln 275 280 285 CCT GAC CCT GCA AGC CAG ATG ATA ACA GTA ACA CAA CTC
AGT CCA ATG 912 Pro Asp Pro Ala Ser Gln Met Ile Thr Val Thr Gln Leu
Ser Pro Met 290 295 300 GAC AGA GAA GCC AGG GTC CTG AGA TAC AGA GAG
AAG AGG AAG ACA AGG 960 Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu
Lys Arg Lys Thr Arg 305 310 315 320 AAA TTT GAG AAG ACA ATA AGG TAT
GCT TCG AGG AAG GCA TAT GCA GAG 1008 Lys Phe Glu Lys Thr Ile Arg
Tyr Ala Ser Arg Lys Ala Tyr Ala Glu 325 330 335 ATA AGA CCG CGG GTC
AAT GGC CGG TTC GCA AAG AGA GAA ATC GAA GCC 1056 Ile Arg Pro Arg
Val Asn Gly Arg Phe Ala Lys Arg Glu Ile Glu Ala 340 345 350 GAG GAG
CAA GGG TTC AAC ACG ATG CTA ATG TAC AAC ACA GGA TAT GGG 1104 Glu
Glu Gln Gly Phe Asn Thr Met Leu Met Tyr Asn Thr Gly Tyr Gly 355 360
365 ATT GTT CCT TCA TTC TGATA 1124 Ile Val Pro Ser Phe 370 (2)
INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 373 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
2: Met Leu Lys Gln Glu Ser Asn Asp Ile Gly Ser Gly Glu Asn Asn Arg
1 5 10 15 Ala Arg Pro Cys Asp Thr Cys Arg Ser Asn Ala Cys Thr Val
Tyr Cys 20 25 30 His Ala Asp Ser Ala Tyr Leu Cys Met Ser Cys Asp
Ala Gln Val His 35 40 45 Ser Ala Asn Arg Val Ala Ser Arg His Lys
Arg Val Arg Val Cys Glu 50 55 60 Ser Cys Glu Arg Ala Pro Ala Ala
Phe Leu Cys Glu Ala Asp Asp Ala 65 70 75 80 Ser Leu Cys Thr Ala Cys
Asp Ser Glu Val His Ser Ala Asn Pro Leu 85 90 95 Ala Arg Arg His
Gln Arg Val Pro Ile Leu Pro Ile Ser Gly Asn Ser 100 105 110 Phe Ser
Ser Met Thr Thr Thr His His Gln Ser Glu Lys Thr Met Thr 115 120 125
Asp Pro Glu Lys Arg Leu Val Val Asp Gln Glu Glu Gly Glu Glu Gly 130
135 140 Asp Lys Asp Ala Lys Glu Val Ala Ser Trp Leu Phe Pro Asn Ser
Asp 145 150 155 160 Lys Asn Asn Asn Asn Gln Asn Asn Gly Leu Leu Phe
Ser Asp Glu Tyr 165 170 175 Leu Asn Leu Val Asp Tyr Asn Ser Ser Met
Asp Tyr Lys Phe Thr Gly 180 185 190 Glu Tyr Ser Gln His Gln Gln Asn
Cys Ser Val Pro Gln Thr Ser Tyr 195 200 205 Gly Gly Asp Arg Val Val
Pro Leu Lys Leu Glu Glu Ser Arg Gly His 210 215 220 Gln Cys His Asn
Gln Gln Asn Phe Gln Phe Asn Ile Lys Tyr Gly Ser 225 230 235 240 Ser
Gly Thr His Tyr Asn Asp Asn Gly Ser Ile Asn His Asn Ala Tyr 245 250
255 Ile Ser Ser Met Glu Thr Gly Val Val Pro Glu Ser Thr Ala Cys Val
260 265 270 Thr Thr Ala Ser His Pro Arg Thr Pro Lys Gly Thr Val Glu
Gln Gln 275 280 285 Pro Asp Pro Ala Ser Gln Met Ile Thr Val Thr Gln
Leu Ser Pro Met 290 295 300 Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg
Glu Lys Arg Lys Thr Arg 305 310 315 320 Lys Phe Glu Lys Thr Ile Arg
Tyr Ala Ser Arg Lys Ala Tyr Ala Glu 325 330 335 Ile Arg Pro Arg Val
Asn Gly Arg Phe Ala Lys Arg Glu Ile Glu Ala 340 345 350 Glu Glu Gln
Gly Phe Asn Thr Met Leu Met Tyr Asn Thr Gly Tyr Gly 355 360 365 Ile
Val Pro Ser Phe 370 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 2493 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
DNA (genomic) (vi) ORIGINAL SOURCE: (A) ORGANISM: Zea mays (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 3: AAGCTTGGGC GTAGGTGTTG
TCTATCGGCG AAAACACGCG CGGTACGCCA AGAACAGCGC 60 GGCCATCTCC
ATCCCAGGCA CGGTGCGCCC GCTTTTTCGC CGTCTCGCTG AGTCACGGC 120
GGCGTCCAGC AGGTAGTTGA GCGCCTTCCG CGGCACGAAT CGCTGCGTGC GGCCCGGAT
180 TGGTCGAGTT GGTAGTCAGC GTCGGTGTCG AATGCCGGGA CGTCGACCAG
GAAGAAGTT 240 CCGTCGCTGG GGTGGGGACG GAAGGCGTCA GGATTGTCGC
AAGGGCAGAG CCCAGCCTG 300 GGGCGGGGCT ACCTCGTCGA CGCCTCGGCA
CGGCGGCGGC AAAGCTGCTG CGGGACGTG 360 CCGCCTGGGC CGCCTTCTCG
GTGAAGTGGT CCTCGAAGGG GACGAGCTCG CTGGGGTCA 420 ACCACCCCAT
AGCTCGAGTC ACCGAAGAAG GCGACGAGGA CGAGCCCGTC GCGGTGGCC 480
CGGTGTACCT CCTCGTCGTC GGTGAGGCTG ACGCTGTAGA TATGGCCAGG CCACCACGG
540 TGGGACTTCA CCTTGGCCCA GACCATGTCG CCGAACCGGG GGCCGCCGTT
CGCCCATGC 600 ATGCCGCGTC CGGCAGCAGG AACCATGGCG CCTCCAGCGG
CGGGGTCGGA CATCCTGTG 660 AGGGGAACCG AAAACCTAGA TTTGGATGCA
GGTTCGATTG GTCTGGGCTT GGGTTTGGG 720 TCCGGAGGAG GGTGGCCTGG
GATCGGTGGA AGGAGGGACA TTGTTGGTAA TTTTTATTA 780 TTTATAATAT
GGAGAAATTC GAGAGACTGA ACGATGGTGA TGTTTATTTG AGGACTATG 840
AGTATAAAGT GTAAAATAGT ATTTTATCAA GTTTATATTC ACGTTTTTGC TGAAGATAG
900 ATAATAGTGG AGTTGTTTTT GGCGGCTACA TAATCTTAGG CTATCTTCTC
GGTCGCTCT 960 ATATCATATC TACTATCACA TTCTCTATTT TAAATTTCAC
TTTGTGTAAT CTACACTA 1020 AAATAGTGTT TTACACGGTA TGTTGTACAC
AGCCTTATCG TGGCGCGACG GAGTTGGA 1080 GAGATGGTGA ACAGCTGGAT
AGATATGATT TATAGGCGAT TGGGTAGATG TGATTTGA 1140 GGTGGTTATG
TAGGAGCGAT TTAGTGAGAC ATTGTAAATA ATTAGGTTGA TGTGATCC 1200
GGATGGCTAG GTAGATATGA TTTTAATGGA TGGTTTGGTG GACTAAGTTA TGTGGACA
1260 ATAATATGTT TTAAATTTCT AAGAAATTGT TTGTGTTAAA TTGTATCCCA
CATAGATT 1320 TTAGCCATCT CAAAGAGAGG TTTGGGTTGT TTACACAAAT
AAAATATTCG TTTGCTTC 1380 CAATTTATAT GTTTTTTATT TACATGAAAA
CTATATTTTT TATTCATCTA CTCACCCA 1440 ACAGAAATTC TGGTTGAGTA
GATGAAAAAA AACTACAACA AACTCTTCCT GAAAGTGT 1500 GTGTGAAGCC
GAGAAATCCT TTTCATTTCG GTGACGGAGC CCCTTGCTGG CTGCTGCT 1560
GTGCACTCCG TTCGCCTGCC TGCCACTACA AGCGACGGCC GACGACTCGC AAGTATCG
1620 AGGCATTTTA AAACTGAAAA CCAAATCTAA ACCCGAATAG ACCAAATTGT
TGGTTTAT 1680 GGGTTTTTGG GTTCGGATTC GGTTTCTAAA TATGCTATAT
TTTAGGGTAT AGGTTCGG 1740 TCAGTTTCTA ACCTTTAAAA CCTGAATAGA
CGAATAACCC GAAATATAAA AAATCTCT 1800 ATATGTGATG ATATTATTAT
ATGATTTATG AACTTATTAA CCGAAAATAA TGATACCA 1860 CTAACGATAG
TATATATATC TATGTATGCT ATTTTTATAG TCACTTGTTG TAATAATA 1920
ACTTCCAATT AATTAATCAG TGTATATATT TTAACAAAAG ATACTAGCCT CTCTACTA
1980 TGAGTATATT CGGTGCACCG AATAGACCGA ACCGAAATTG TAAGTCTATT
CAGGTTCG 2040 TCCTAAAATT ATTTTAAAAA TTTTGGTTCT CATATTTCAG
AATCCGAAAT TTCATAAA 2100 CAAATAGACC GAACCAAATT ACGCTAATAG
ACCGAATAAC TAGCGTACTC GCAAGTCG 2160 CCCCACTAGC CTGCTGCGTG
CGTAAGCGAG GACGTCACGC GTTCTCCCTC CCGTCGAC 2220 AATACACTTG
GTCTTCTAGC ACCTTCTTCC TCTCCAAGAC TCCAATCCCC CAACCACC 2280
AACCAGCGCC AGCTCTAACG TCACCTCTGA TTTCTCTCTC CTCTCTATTG CTAGCTGC
2340 TATTATAAGT AGCAGCTGCA GCAGGCAGGA GCTGCACACA CCCATCCAAT
TCCAGCTG 2400 GATCTTGATC CTGCACCCCG AGCCGTACAC AAGAGCTAGT
CGGTAGAACT TGCAGGAG 2460 GAGCAGAACT AAGTGCAGAG AACAGGACAT ATG 2493
(2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 4201 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi)
ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis thaliana (B) STRAIN:
Landsberg erecta (viii) POSITION IN GENOME: (B) MAP POSITION:
chromosome 5 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: GAATTCATGT
ACCAAATCAA TACTTTTTAG CCATAAATGA GTCAGTTTTA GTATCCACAT 60
GAATTTACCT ACCAGAGTGT TGTAAATTAT GTTCTTTTGG GGCCACTTAC ATGGATCTC
120 TTCATTCACT GCAGCGAGTT CTCAGACCAC CAGAAAATTT ATTCAGTGAT
CTGTTTTGA 180 CATGCAACAT AAACTTATAA GCCACACAAG CAAAACAAAG
ATATCCCATG TTGCATATA 240 TACGAGCTAG CATATCATAA AGAAGGAAAC
TTGAAGTAGC AAAGTTTCTA CTAAATTTC 300 TGTCAGGAAT TTTTAAAATG
CAATGACAAC CACTTGGAGC ACTATGAGTT TCAGAGCCA 360 TAGAATGTTA
CTATTTGGTG TGGATTCGAG CTAGCACGTG AAAGTGCATA AAAGTGATT 420
CCTTTTGCCA AAGGTCACTG CACTTTTCCT CAGATAGTTT CTCACAGCCA TGGAAAGTG
480 AGAATCCGCA TAAACGTACA ATTACAAGCT TTATATGGTC CCTCGACTCT
TATTCTCTT 540
TCAGTCTTTG CAACTAAATA GGGTTTTCGT TAATCTGAAA GAAGCAAAGT ATTCGAAAC
600 ACGGAAACCT GATAAAGAAT GAAAACAAAT AAGCAATAGT GTTTTCTTGA
AAATCTCGA 660 GCAACTTTGA GGATATTGTT ACATATGATC TATTACTCGT
AACAGTTATC CGAAGGCCT 720 CACATGTGAG AGAAGTTCCA AACCGCTACA
ACAATAAACT TAATTAGAGA CTGTCAACG 780 GCAATAATAA GCAAAACTAC
TTTTTTCTTG AGCTACAAGT GAAAAGGCCA ATACACAAT 840 TACTCTTCAT
GAACTCGAAC CACGTTACAA TCTCCAAAAA ATTTCATCAC CAAAGCACT 900
AAAGCCAAAG ATGCCTCAAC TTATCCAACT TGGCAGGATA AAGATCTCCA AAAATGCTT
960 CTAAAGAACC TAGAATCTTT TCTTTAGAAT TCAATGATCA TATAACCATT TCATAACA
1020 TCTAAATGCC ATTACATTCA TCGTAAAACC AGTAAATAAC AAGAACTTGT
ATGTTAAG 1080 CCAATTACCA AGCAAAAAAA AACTTTTCAA AGTTTAAAGT
TCAAAATGGG AAAGAGAA 1140 GCGGTGTAAG CAAATATGAA AGAGGAAGAG
ATGCGAAAAG TGTATCCTAG GACCAGCA 1200 TTATACAAAA AAAAAACACT
CACTTTTCAG CTCTTAAGGC ATAGAGTGAA GGTAGCCA 1260 TGAATTTGGC
CACTAGAGCG TCCGTCAAAT CTCATTCTTT TTGGACCACA TAATGGGT 1320
CATACATTCA CTGGACCCAA AAGCGTAACT GGAGCTAGTC CTCAAACCTA GAGAGTAT
1380 TATCCTGTAG CTTCCACATA GTAAACATTA TGAGCATAAC ACCAACAAGG
CAACTCCA 1440 TACTAATGGT TATTAGTACA GGAAAACCCA CGATGCTAAA
CACATGAATG GGTCACCA 1500 TAGAGTGAAG ATGGTTAAAT TGCATCTATG
GATCATGTGG ACTAGTAAAT GAGTGTAG 1560 GAAAACTTCA CAATTACCTC
TGTGATCTTA GAAACATGTC CTGAAAATTC CATACAAG 1620 TCGTTTGTAT
TAGATTACTT CCACAGGTTG AGATCTAATA AAGCTACAAT AAATAGTA 1680
GAGTATCATC ATAAACCCAA ATTACAGAGA TGTGACAACA CTCATGAGTC ATGTTTTG
1740 ACTACTTACT ATAATGGTTA CCAAGTGCAA ATTTCTACAT ACTATATATG
ATAAATCT 1800 TTATTGCTCA TGTGGACTCC AAAATGCCTT TTAAGTTTTA
ACTTGTGCGT CAGGTAAA 1860 CTAATTTGTA GTCTCAAGAC TACTTGGCGG
ATTCGAGTTT GATCCTAGAA AATCCACC 1920 CTCTATGTTT TTCATGTCAC
TTTTCCGATA TGATTCTCAT TACCATGACT TTATGAAC 1980 GATTAAACAT
TATAACACTT TTCATCAGAA AATCCTTCGA AAGTTTCAAT TGCAAATC 2040
TCTAAATGAT GCAGATGCAT TCACAAATAA TGGAACAACA ACTATACCAT ATTCACGA
2100 TTGTCTAACC TTTGTATAGG TAGTCAACCC ATAACAGTTG GTGATGGCTC
TGACACTC 2160 AGCCTTACTC GGAGAGATAC CTGAACAGTA ATCACAAGGT
TCAGGATGAA TATTCAAC 2220 CTTAAACTTT GTATAAAGCC AAAGAGATAA
AACGAATCTA GCTTTACTTT AAATAAAA 2280 CATATGAAAA TAGTAAAAGG
TGATACGAAA AAATAGTAAC AATTTGCCTG CAACACCA 2340 GCATTATCCG
GACCACTTCC TCTTGAGAAT CTCAGTATGG CAAGTGGCAA AACCTAAG 2400
ACTTGTGAAC GGGTCCCAAC GAAGAAGTGC ATAGGAGGAG ATGTTTACAC TTTACACT
2460 ACACTTTACA CTTTACACAT AGGCCTTCCC AAAAGCTCAA CTAGCTGCAA
GAGGATCC 2520 TAACATGTAA GAGCCACTAA CGCTGTGCCA CGTGTAGGCA
CTCAGGATTC GATCTTCC 2580 TCTACTTATT CTCTCACACC AGATATAAGC
TTTATTAGCC CCTTCTTTCA GATACCAG 2640 CCCACACCAT CAAACTTACT
ACATCTGAGT TATTATGTTG AAACAAGAGA GTAACGAC 2700 AGGTAGTGGA
GAGAACAACA GGGCACGACC CTGTGACACA TGCCGGTCAA ACGCCTGC 2760
CGTGTATTGC CATGCAGATT CTGCCTACTT GTGCATGAGC TGTGATGCTC AAGTTCAC
2820 TGCCAATCGC GTTGCTTCCC GCCATAAACG TGTCCGGGTC TGCGAGTCAT
GTGAGCGT 2880 TCCGGCTGCT TTTTTGTGTG AGGCAGATGA TGCCTCTCTA
TGCACAGCCT GTGATTCA 2940 GGTTCATTCT GCAAACCCAC TTGCTAGACG
CCATCAGCGA GTTCCAATTC TACCAATT 3000 TGGAAACTCT TTCAGCTCCA
TGACCACTAC TCACCACCAA AGCGAGAAAA CAATGACC 3060 TCCAGAGAAG
AGACTGGTGG TGGATCAAGA GGAAGGTGAA GAAGGTGATA AGGATGCC 3120
GGAGGTTGCT TCGTGGCTGT TCCCTAATTC AGACAAAAAT AACAATAACC AAAACAAT
3180 GTTATTGTTT AGTGATGAGT ATCTAAACCT TGTGGATTAC AACTCGAGTA
TGGACTAC 3240 ATTCACAGGT GAATACAGTC AACACCAACA AAACTGCAGC
GTACCACAGA CGAGCTAC 3300 GGGAGATAGA GTTGTTCCGC TTAAACTTGA
AGAATCAAGG GGCCACCAGT GCCATAAC 3360 ACAGAATTTT CAGTTCAATA
TCAAATATGG CTCCTCAGGG ACTCACTACA ACGACAAT 3420 TTCCATTAAC
CATAACGTAA GGCTTTTGTA TATTTGTTAC CCCTTCAATT TAGCATCT 3480
CCATAACGCA GCAGGGTGAA TTCTTTCATC ATACACACAA ATCCACTGAT CCACTGCC
3540 CAGTTGATCT ATAGCACATA GAAATTTCAC CAGAAGTCTA TAATAAAAAC
AATATATG 3600 TCCTTTTGCA TCGACTCTCT TTAGTCCTCT TACCAGGGGG
ATTGAGAATG TCTTTGTT 3660 TGTCATTAGG CATACATTTC ATCCATGGAA
ACTGGTGTTG TGCCGGAGTC AACAGCAT 3720 GTCACAACAG CTTCACACCC
AAGAACGCCC AAAGGGACAG TAGAGCAACA ACCTGACC 3780 GCAAGCCAGA
TGATAACAGT AACACAACTC AGTCCAATGG ACAGAGAAGC CAGGGTCC 3840
AGATACAGAG AGAAGAGGAA GACAAGGAAA TTTGAGAAGA CAATAAGGTA TGCTTCGA
3900 AAGGCATATG CAGAGATAAG ACCGCGGGTC AATGGCCGGT TCGCAAAGAG
AGAAATCG 3960 GCCGAGGAGC AAGGGTTCAA CACGATGCTA ATGTACAACA
CAGGATATGG GATTGTTC 4020 TCATTCTGAT ACTCCTGTGG CAAAAAGAAA
AACTAGATTG CAAGCTGTAA ATTACTTT 4080 GTTTGAGATT ATGTTAGGTT
TGGTGAAATT CTTAGCTTCA AGAAGTATTA CTACTGTT 4140 GCAAATGGGT
TTGTAGTTTT GGCTAATTAA AACTATAGTA TTCTTCTTTC TCTGCATT 4200 T 4201
(2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 1101 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii)
HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Brassic napus
(ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1098 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 5: ATG TTC AAA CAA GAG AGT AAC AAC ATT GGT
AGT GAA GAG AAC AAC ACC 48 Met Phe Lys Gln Glu Ser Asn Asn Ile Gly
Ser Glu Glu Asn Asn Thr 375 380 385 GGG GCG CGA GCT TGT GAC ACA TGC
GGG TCA ACC ATC TGC ACC GTG TAC 96 Gly Ala Arg Ala Cys Asp Thr Cys
Gly Ser Thr Ile Cys Thr Val Tyr 390 395 400 405 TGC CAT GCT GAC TCC
GCC TAC TTA TGC AAT AGC TGC GAT GCT CAA GTC 144 Cys His Ala Asp Ser
Ala Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 410 415 420 CAC TCT GCC
AAT CGC GTT GCT TCC CGC CAT AAA AGG GTC AGA GTG TGC 192 His Ser Ala
Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 425 430 435 GAG
TCA TGT GAG CGT GCC CCT GCT GCT TTT ATG TGT GAG GCA GAT GAT 240 Glu
Ser Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 440 445
450 GTG TCT CTA TGC ACA GCC TGT GAT TCA GAG GTT CAC TCC GCA AAC CCT
288 Val Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala Asn Pro
455 460 465 CTT GCT AGA CGC CAT CAG CGA GTT CCA GTT GTG CCG ATA ACT
GGA AAC 336 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro Ile Thr
Gly Asn 470 475 480 485 TCT TGC AGC TCC TTG GCC ACC GCT AAC CAC ACA
ACA GTG ACC GAG CCA 384 Ser Cys Ser Ser Leu Ala Thr Ala Asn His Thr
Thr Val Thr Glu Pro 490 495 500 GAG AAG AGA GTG GTG TTA GTT CAA GAG
GAT GCC AAA GAG ACG GCT TCA 432 Glu Lys Arg Val Val Leu Val Gln Glu
Asp Ala Lys Glu Thr Ala Ser 505 510 515 TGG TTG TTC CCT AAA AAC AGT
GAC AAT CAC AAC AAC AAC AAC CAG AAC 480 Trp Leu Phe Pro Lys Asn Ser
Asp Asn His Asn Asn Asn Asn Gln Asn 520 525 530 AAT GAG TTG TTG TTT
AGT GAT GAC TAT CTA GAC CTT GCT GAT TAC AAC 528 Asn Glu Leu Leu Phe
Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr Asn 535 540 545 TCG AGT ATG
GAC TAC AAG TTC ACT GGT CAA TAC AAT CAA CCT ACT CAA 576 Ser Ser Met
Asp Tyr Lys Phe Thr Gly Gln Tyr Asn Gln Pro Thr Gln 550 555 560 565
CAT AAA CAA GAC TGC ACC GTA CCA GAG AAA AAC TAC GGT GGA GAT AGA 624
His Lys Gln Asp Cys Thr Val Pro Glu Lys Asn Tyr Gly Gly Asp Arg 570
575 580 GTT GTT CCA CTC CAA CTT GAA GAA ACA AGA GGA AAC TTG CAC CAC
AAG 672 Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu His His
Lys 585 590 595 CAA CAT AAT ATC ACG TAT GGC TCC TCA GGA AGT CAC TAC
AAC AAC AAT 720 Gln His Asn Ile Thr Tyr Gly Ser Ser Gly Ser His Tyr
Asn Asn Asn 600 605 610 GGT TCC ATA AAC CAT AAC GCA TAC AAT CCA TCA
ATG GAA ACT GAC TTT 768 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser
Met Glu Thr Asp Phe 615 620 625 GTT CCG GAG CAG ACA GCA CCT GAC AAA
ACA GTT TCA CAT CCA AAA ACG 816 Val Pro Glu Gln Thr Ala Pro Asp Lys
Thr Val Ser His Pro Lys Thr 630 635 640 645 CAC AAA GGG AAG ATA GAG
AAA CTA CCT GAA CCT CTA ATT CAG ATT CTC 864 His Lys Gly Lys Ile Glu
Lys Leu Pro Glu Pro Leu Ile Gln Ile Leu 650 655 660 AGT CCA ATG GAC
AGA GAA GCT AGA GTC CTG AGA TAC AGA GAG AAG AAG 912 Ser Pro Met Asp
Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys 665 670 675 AAG AGA
AGA AAG TTT GAG AAG ACA ATA AGG TAT GCT TCA AGG AAG GCA 960 Lys Arg
Arg Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 680 685 690
TAT GCA GAG AGA AGA CCG AGG ATC AAT GGA CGG TTT GCA AAG ATT AGT
1008 Tyr Ala Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Ile
Ser 695 700 705 GAA ACC GAA GTA GAG GAC CAA GAG TAC AAC ACA ATG CTA
ATG TAC TAT 1056 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met
Leu Met Tyr Tyr 710 715 720 725 GAC ACA GGA TAT GGC ATT GTT CCT TCA
TTC TAT GGC CAA AAA 1098 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe
Tyr Gly Gln Lys 730 735 TAA 1101 (2) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 366 amino acids (B) TYPE:
amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 6:
Met Phe Lys Gln Glu Ser Asn Asn Ile Gly Ser Glu Glu Asn Asn Thr 1 5
10 15 Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr Ile Cys Thr Val
Tyr 20 25 30 Cys His Ala Asp Ser Ala Tyr Leu Cys Asn Ser Cys Asp
Ala Gln Val 35 40 45 His Ser Ala Asn Arg Val Ala Ser Arg His Lys
Arg Val Arg Val Cys 50 55 60 Glu Ser Cys Glu Arg Ala Pro Ala Ala
Phe Met Cys Glu Ala Asp Asp 65 70 75 80 Val Ser Leu Cys Thr Ala Cys
Asp Ser Glu Val His Ser Ala Asn Pro 85 90 95 Leu Ala Arg Arg His
Gln Arg Val Pro Val Val Pro Ile Thr Gly Asn 100 105 110 Ser Cys Ser
Ser Leu Ala Thr Ala Asn His Thr Thr Val Thr Glu Pro 115 120 125 Glu
Lys Arg Val Val Leu Val Gln Glu Asp Ala Lys Glu Thr Ala Ser 130 135
140 Trp Leu Phe Pro Lys Asn Ser Asp Asn His Asn Asn Asn Asn Gln Asn
145 150 155 160 Asn Glu Leu Leu Phe Ser Asp Asp Tyr Leu Asp Leu Ala
Asp Tyr Asn 165 170 175 Ser Ser Met Asp Tyr Lys Phe Thr Gly Gln Tyr
Asn Gln Pro Thr Gln 180 185 190 His Lys Gln Asp Cys Thr Val Pro Glu
Lys Asn Tyr Gly Gly Asp Arg 195 200 205 Val Val Pro Leu Gln Leu Glu
Glu Thr Arg Gly Asn Leu His His Lys 210 215 220 Gln His Asn Ile Thr
Tyr Gly Ser Ser Gly Ser His Tyr Asn Asn Asn 225 230 235 240 Gly Ser
Ile Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp Phe 245 250 255
Val Pro Glu Gln Thr Ala Pro Asp Lys Thr Val Ser His Pro Lys Thr 260
265 270 His Lys Gly Lys Ile Glu Lys Leu Pro Glu Pro Leu Ile Gln Ile
Leu 275 280 285 Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg
Glu Lys Lys 290 295 300 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg Tyr
Ala Ser Arg Lys Ala 305 310 315 320 Tyr Ala Glu Arg Arg Pro Arg Ile
Asn Gly Arg Phe Ala Lys Ile Ser 325 330 335 Glu Thr Glu Val Glu Asp
Gln Glu Tyr Asn Thr Met Leu Met Tyr Tyr 340 345 350 Asp Thr Gly Tyr
Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys 355 360 365 NFORMATION FOR
SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1101 base
pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY:
linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: YES
(vi) ORIGINAL SOURCE: (A) ORGANISM: Brassica napus (ix) FEATURE:
(A) NAME/KEY: CDS (B) LOCATION:1..1098 (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 7: ATG TTC AAA CAA GAG AGT AAC AAC ATT TGT AAT AGA GAG
AAC AAC AGA 48 Met Phe Lys Gln Glu Ser Asn Asn Ile Cys Asn Arg Glu
Asn Asn Arg 370 375 380 GGG GCA CGA GCC TGT GAC ACA TGC GGG TCA ACC
ATC TGC ACC GTG TAC 96 Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr
Ile Cys Thr Val Tyr 385 390 395 TGC CAT GCT GAC TCT GCC TAC TTA TGC
AAT AGC TGC GAT GCT CAA GTC 144 Cys His Ala Asp Ser Ala Tyr Leu Cys
Asn Ser Cys Asp Ala Gln Val 400 405 410 CAC TCT GCC AAT CGC GTT GCT
TCC CGC CAT AAA CGT GTC CGG GTC TGC 192 His Ser Ala Asn Arg Val Ala
Ser Arg His Lys Arg Val Arg Val Cys 415 420 425 430 GAG TCA TGT GAG
CGT GCC CCT GCT GCT TTT ATG TGT GAG GCA GAT GAT 240 Glu Ser Cys Glu
Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 435 440 445 GTG TCT
CTA TGC ACA GCC TGT GAT TTA GAG GTT CAC TCC GCA AAC CCT 288 Val Ser
Leu Cys Thr Ala Cys Asp Leu Glu Val His Ser Ala Asn Pro 450 455 460
CTT GCT AGA CGC CAT CAG CGA GTT CCA GTT GTG CCG ATA ATT GGA AAC 336
Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro Ile Ile Gly Asn 465
470 475 TCT TGC AGC TCC TTG GCC ACC GCT AAC CAC ACA ACA GTG ACC GAG
CCA 384 Ser Cys Ser Ser Leu Ala Thr Ala Asn His Thr Thr Val Thr Glu
Pro 480 485 490 GAG AAG AGA GTG GTG TTA GTT CAA GAG GAT GCC AAA GAG
ACG GCT TCA 432 Glu Lys Arg Val Val Leu Val Gln Glu Asp Ala Lys Glu
Thr Ala Ser 495 500 505 510 TGG TTG TTC CCT AAA AAC AGT GAC TAT CAC
AAC AAC AAC AAC AAC CAG 480 Trp Leu Phe Pro Lys Asn Ser Asp Tyr His
Asn Asn Asn Asn Asn Gln 515 520 525 AAC AAT GAG TTG TTG TTT AGT GAT
GAC TAC CTA GAC CTT GCT GAT TAC 528 Asn Asn Glu Leu Leu Phe Ser Asp
Asp Tyr Leu Asp Leu Ala Asp Tyr 530 535 540 AAC TCC AGT ATG GAC TAC
AAG TTC ACC AGT CAA TAC AAT CAA CCT CGA 576 Asn Ser Ser Met Asp Tyr
Lys Phe Thr Ser Gln Tyr Asn Gln Pro Arg 545 550 555 CAT AAA CAA GAC
TGC ATC GTA CCA GAG AAA AAC TAC AGT GGA GAT AGA 624 His Lys Gln Asp
Cys Ile Val Pro Glu Lys Asn Tyr Ser Gly Asp Arg 560 565 570 GTT GTT
CCG CTC CAA CTT GAA GAA ACA AGA GGA AAC TTG CGG AAC AAG 672 Val Val
Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu Arg Asn Lys 575 580 585
590 CAA CAG AAT ATC ACA TAT GGC TCC TCA GGA AGC CAA TAC AAC AAC AAC
720 Gln Gln Asn Ile Thr Tyr Gly Ser Ser Gly Ser Gln Tyr Asn Asn Asn
595 600 605 GGT TCC ATT AAC CAT AAC GCA TAC AAT CCA TCA ATG GAA ACT
GAC TTT 768 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr
Asp Phe 610 615 620 GTG CCG GAG CAG ACA GCA CCT GAC ACA ACA GTT TCA
CAT CCA AAA ACG 816 Val Pro Glu Gln Thr Ala Pro Asp Thr Thr Val Ser
His Pro Lys Thr 625 630 635 CAC AAA GGG AAG ACA GCA CAA CTA CCT GAA
CCT CTA ATT CAG ATT CTC 864 His Lys Gly Lys Thr Ala Gln Leu Pro Glu
Pro Leu Ile Gln Ile Leu 640 645 650 AGT CCA ATG GAC AGA GAA GCT AGA
GTC CTG AGA TAC AGA GAG AAG AAG 912 Ser Pro Met Asp Arg Glu Ala Arg
Val Leu Arg Tyr Arg Glu Lys Lys 655 660 665 670 AAG AGA AGA AAG TTT
GAG AAG ACA ATA AGG TAT GCT TCA AGG AAG GCA 960 Lys Arg Arg Lys Phe
Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 675 680 685 TAT GCA GAG
AGA AGA CCG AGG ATA AAT GGA CGG TTT GCA AAG ATG AGT 1008 Tyr Ala
Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Met Ser 690 695 700
GAA ACC GAA GTA GAG GAC CAA GAG TAC AAC ACA ATG CTA ATG TAC TGC
1056 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr
Cys 705 710 715 GAC ACA GGA TAT GGC ATT GTT CCT TCA TTC TAT GGC CAA
AAA 1098 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys
720 725 730 TAA 1101 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 366 amino acids (B) TYPE: amino acid
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 8: Met Phe Lys Gln Glu Ser Asn Asn Ile Cys
Asn Arg Glu Asn Asn Arg 1 5 10 15 Gly Ala Arg Ala Cys Asp Thr Cys
Gly Ser Thr Ile Cys Thr Val Tyr 20 25 30 Cys His Ala Asp Ser Ala
Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 35 40 45 His Ser Ala Asn
Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 50 55 60 Glu Ser
Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 65 70 75 80
Val Ser Leu Cys Thr Ala Cys Asp Leu Glu Val His Ser Ala Asn Pro 85
90 95 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro Ile Ile Gly
Asn 100 105 110 Ser Cys Ser Ser Leu Ala Thr Ala Asn His Thr Thr Val
Thr Glu Pro 115 120 125 Glu Lys Arg Val Val Leu Val Gln Glu Asp Ala
Lys Glu Thr Ala Ser 130 135 140 Trp Leu Phe Pro Lys Asn Ser Asp Tyr
His Asn Asn Asn Asn Asn Gln 145 150 155 160 Asn Asn Glu Leu Leu Phe
Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr 165 170 175 Asn Ser Ser Met
Asp Tyr Lys Phe Thr Ser Gln Tyr Asn Gln Pro Arg 180 185 190 His Lys
Gln Asp Cys Ile Val Pro Glu Lys Asn Tyr Ser Gly Asp Arg 195 200 205
Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu Arg Asn Lys 210
215 220 Gln Gln Asn Ile Thr Tyr Gly Ser Ser Gly Ser Gln Tyr Asn Asn
Asn 225 230 235 240 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met
Glu Thr Asp Phe 245 250 255 Val Pro Glu Gln Thr Ala Pro Asp Thr Thr
Val Ser His Pro Lys Thr 260 265 270 His Lys Gly Lys Thr Ala Gln Leu
Pro Glu Pro Leu Ile Gln Ile Leu 275 280 285
Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys 290
295 300 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys
Ala 305 310 315 320 Tyr Ala Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe
Ala Lys Met Ser 325 330 335 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn
Thr Met Leu Met Tyr Cys 340 345 350 Asp Thr Gly Tyr Gly Ile Val Pro
Ser Phe Tyr Gly Gln Lys 355 360 365 NFORMATION FOR SEQ ID NO: 9:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 9: TCCTGCTCGC TTCGCTACTT 20 (2)
INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
10: GCGATGCTGT CGGAATGGAC 20 (2) INFORMATION FOR SEQ ID NO: 11: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 11: AAGTACTCTC GGTAGCCAAG 20 (2)
INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for pcr" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
12: GTGTGGTCGC CATGATCGCG 20 (2) INFORMATION FOR SEQ ID NO: 13: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 13: CTGGGAAGTG AATGGAGACA TA 22
(2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
14: AGGAGTCGCA TAAGGGAGAG 20 (2) INFORMATION FOR SEQ ID NO: 15: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 15: ACGTCGGATG CTCACTATAG GGATC 25
(2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
16: GTGATAAACT ACCGCATTAA AGC 23 (2) INFORMATION FOR SEQ ID NO: 17:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 17: ACGTCGGATG ACTTTAATTT ATTCACTA
28 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
18: GACGTGGATG CTCACTAAAG GGATC 25 (2) INFORMATION FOR SEQ ID NO:
19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: AGAGCCTTCA ACCCAGTCAG 20
(2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
20: ACGTCGGATG CCGATCTCAA GATTA 25 (2) INFORMATION FOR SEQ ID NO:
21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A)
DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL SOURCE:
(A) ORGANISM: Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ
ID NO: 21: GCTCCCACAC CATCAAACTT ACTAC 25 (2) INFORMATION FOR SEQ
ID NO: 22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY:
linear (ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION:
/desc = "primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:
CTCCTCGGCT TCGATTTCTC 20 (2) INFORMATION FOR SEQ ID NO: 23: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: designed primer
not derived from any organism (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
23: GACTCGAGTC GACATCG 17 (2) INFORMATION FOR SEQ ID NO: 24: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
TGCAGATTCT GCCTACTTGT GC 22 (2) INFORMATION FOR SEQ ID NO: 25: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
TGATTCTGCC TACTTGTGCT C 21 (2) INFORMATION FOR SEQ ID NO: 26: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
synthesised for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:
GCTTGGTTTG CCTCTTCATC 20 (2) INFORMATION FOR SEQ ID NO: 27: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
synthesised for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
GGTCCCAACG AAGAAGTGC 19 (2) INFORMATION FOR SEQ ID NO: 28: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
CAGGGAGGCG TGAAAGTGT 19 (2) INFORMATION FOR SEQ ID NO: 29: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "adapter
synthesised to (vi) ORIGINAL SOURCE: (A) ORGANISM: designed
sequence not derived from any organism (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 29: TACAAGCTTG 10 (2) INFORMATION FOR SEQ ID NO: 30: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 12 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"oligonucleotide (vi) ORIGINAL SOURCE: (A) ORGANISM: designed
sequence, not derived from any organism (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 30: TATCGATAGT AC 12 (2) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 31: TTCAAGCTCT ACGCCGGA 18 (2)
INFORMATION FOR SEQ ID NO: 32: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 24 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein (ix) FEATURE: (A) NAME/KEY:
Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= Xaa
/note= Xaa at positions 2, 3, 5-20, 22 and 23 can be any amino acid
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32: Cys Xaa Xaa Cys Xaa Xaa
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa
Cys Xaa Xaa Cys 20 (2) INFORMATION FOR SEQ ID NO: 33: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 25 amino acids (B) TYPE: amino acid
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (ix) FEATURE: (A)
NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION:
/label= Xaa /note= Xaa at positions 2, 3, 5-21, 23 and 24 can be
any amino acid (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33: Cys Xaa
Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10
15
Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Cys 20 25 SEQUENCE LISTING (1)
GENERAL INFORMATION: 1 (i) APPLICANT: Coupland, George M.
Putterill, Joanna J. (ii) TITLE OF INVENTION: Genetic control of
flowering (iii) NUMBER OF SEQUENCES: 33 (iv) CORRESPONDENCE
ADDRESS: (A) ADDRESSEE: Nixon & Vanderhye PC (B) STREET: 8th
Floor, 1100 North Glebe Road 81100 (C) CITY: Arlington (D) STATE:
Virginia (E) COUNTRY: United States of America (F) ZIP: 22201-4714
222014714 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk
(B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS
(D) SOFTWARE: PatentIn Release #1.0, Version #1.25 (EPO) 10125 (vi)
CURRENT APPLICATION DATA: (A) APPLICATION NUMBER:US/11/314,171 (B)
FILING DATE: 22-DEC-2005 222005 (C) CLASSIFICATION: (vii) PRIOR
APPLICATION DATA: (A) APPLICATION NUMBER: US/10/143,849 10143849
(B) FILING DATE: 14-May-2002 142002 (C) CLASSIFICATION: (vii) PRIOR
APPLICATION DATA: (A) APPLICATION NUMBER: US 09/516,191 09516191
(B) FILING DATE: 01-MAR-2000 012000 (A) APPLICATION NUMBER: US
08/945,056 08945056 (B) FILING DATE: 20-OCT-1997 201997 (A)
APPLICATION NUMBER: PCT/GB95/02561 9502561 (B) FILING DATE:
01-NOV-1995 011995 (A) APPLICATION NUMBER: GB 9422083.7 94220837
(B) FILING DATE: 02-NOV-1994 021994 (viii) ATTORNEY/AGENT
INFORMATION: (A) NAME: Ms Mary J Wilson (B) REGISTRATION NUMBER:
32,955 32955 (C) REFERENCE/DOCKET NUMBER: 620-197 620197 (ix)
TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (703) 816-4000
7038164000 (B) TELEFAX: (703) 816-4100 7038164100 (2) INFORMATION
FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1124
base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D)
TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA to mRNA (iii)
HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A)
ORGANISM: Arabidopsis thaliana (B) STRAIN: Landsberg erecta (viii)
POSITION IN GENOME: (A) CHROMOSOME/SEGMENT: chromosome 5 (ix)
FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1119 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 1: ATG TTG AAA CAA GAG AGT AAC GAC ATA GGT
AGT GGA GAG AAC AAC AGG 48 Met Leu Lys Gln Glu Ser Asn Asp Ile Gly
Ser Gly Glu Asn Asn Arg 1 5 10 15 GCA CGA CCC TGT GAC ACA TGC CGG
TCA AAC GCC TGC ACC GTG TAT TGC 96 Ala Arg Pro Cys Asp Thr Cys Arg
Ser Asn Ala Cys Thr Val Tyr Cys 20 25 30 CAT GCA GAT TCT GCC TAC
TTG TGC ATG AGC TGT GAT GCT CAA GTT CAC 144 His Ala Asp Ser Ala Tyr
Leu Cys Met Ser Cys Asp Ala Gln Val His 35 40 45 TCT GCC AAT CGC
GTT GCT TCC CGC CAT AAA CGT GTC CGG GTC TGC GAG 192 Ser Ala Asn Arg
Val Ala Ser Arg His Lys Arg Val Arg Val Cys Glu 50 55 60 TCA TGT
GAG CGT GCT CCG GCT GCT TTT TTG TGT GAG GCA GAT GAT GCC 240 Ser Cys
Glu Arg Ala Pro Ala Ala Phe Leu Cys Glu Ala Asp Asp Ala 65 70 75 80
TCT CTA TGC ACA GCC TGT GAT TCA GAG GTT CAT TCT GCA AAC CCA CTT 288
Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala Asn Pro Leu 85
90 95 GCT AGA CGC CAT CAG CGA GTT CCA ATT CTA CCA ATT TCT GGA AAC
TCT 336 Ala Arg Arg His Gln Arg Val Pro Ile Leu Pro Ile Ser Gly Asn
Ser 100 105 110 TTC AGC TCC ATG ACC ACT ACT CAC CAC CAA AGC GAG AAA
ACA ATG ACC 384 Phe Ser Ser Met Thr Thr Thr His His Gln Ser Glu Lys
Thr Met Thr 115 120 125 GAT CCA GAG AAG AGA CTG GTG GTG GAT CAA GAG
GAA GGT GAA GAA GGT 432 Asp Pro Glu Lys Arg Leu Val Val Asp Gln Glu
Glu Gly Glu Glu Gly 130 135 140 GAT AAG GAT GCC AAG GAG GTT GCT TCG
TGG CTG TTC CCT AAT TCA GAC 480 Asp Lys Asp Ala Lys Glu Val Ala Ser
Trp Leu Phe Pro Asn Ser Asp 145 150 155 160 AAA AAT AAC AAT AAC CAA
AAC AAT GGG TTA TTG TTT AGT GAT GAG TAT 528 Lys Asn Asn Asn Asn Gln
Asn Asn Gly Leu Leu Phe Ser Asp Glu Tyr 165 170 175 CTA AAC CTT GTG
GAT TAC AAC TCG AGT ATG GAC TAC AAA TTC ACA GGT 576 Leu Asn Leu Val
Asp Tyr Asn Ser Ser Met Asp Tyr Lys Phe Thr Gly 180 185 190 GAA TAC
AGT CAA CAC CAA CAA AAC TGC AGC GTA CCA CAG ACG AGC TAC 624 Glu Tyr
Ser Gln His Gln Gln Asn Cys Ser Val Pro Gln Thr Ser Tyr 195 200 205
GGG GGA GAT AGA GTT GTT CCG CTT AAA CTT GAA GAA TCA AGG GGC CAC 672
Gly Gly Asp Arg Val Val Pro Leu Lys Leu Glu Glu Ser Arg Gly His 210
215 220 CAG TGC CAT AAC CAA CAG AAT TTT CAG TTC AAT ATC AAA TAT GGC
TCC 720 Gln Cys His Asn Gln Gln Asn Phe Gln Phe Asn Ile Lys Tyr Gly
Ser 225 230 235 240 TCA GGG ACT CAC TAC AAC GAC AAT GGT TCC ATT AAC
CAT AAC GCA TAC 768 Ser Gly Thr His Tyr Asn Asp Asn Gly Ser Ile Asn
His Asn Ala Tyr 245 250 255 ATT TCA TCC ATG GAA ACT GGT GTT GTG CCG
GAG TCA ACA GCA TGT GTC 816 Ile Ser Ser Met Glu Thr Gly Val Val Pro
Glu Ser Thr Ala Cys Val 260 265 270 ACA ACA GCT TCA CAC CCA AGA ACG
CCC AAA GGG ACA GTA GAG CAA CAA 864 Thr Thr Ala Ser His Pro Arg Thr
Pro Lys Gly Thr Val Glu Gln Gln 275 280 285 CCT GAC CCT GCA AGC CAG
ATG ATA ACA GTA ACA CAA CTC AGT CCA ATG 912 Pro Asp Pro Ala Ser Gln
Met Ile Thr Val Thr Gln Leu Ser Pro Met 290 295 300 GAC AGA GAA GCC
AGG GTC CTG AGA TAC AGA GAG AAG AGG AAG ACA AGG 960 Asp Arg Glu Ala
Arg Val Leu Arg Tyr Arg Glu Lys Arg Lys Thr Arg 305 310 315 320 AAA
TTT GAG AAG ACA ATA AGG TAT GCT TCG AGG AAG GCA TAT GCA GAG 1008
Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala Tyr Ala Glu 325
330 335 ATA AGA CCG CGG GTC AAT GGC CGG TTC GCA AAG AGA GAA ATC GAA
GCC 1056 Ile Arg Pro Arg Val Asn Gly Arg Phe Ala Lys Arg Glu Ile
Glu Ala 340 345 350 GAG GAG CAA GGG TTC AAC ACG ATG CTA ATG TAC AAC
ACA GGA TAT GGG 1104 Glu Glu Gln Gly Phe Asn Thr Met Leu Met Tyr
Asn Thr Gly Tyr Gly 355 360 365 ATT GTT CCT TCA TTC TGATA 1124 Ile
Val Pro Ser Phe 370 (2) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 373 amino acids (B) TYPE: amino acid
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 2: Met Leu Lys Gln Glu Ser Asn Asp Ile Gly
Ser Gly Glu Asn Asn Arg 1 5 10 15 Ala Arg Pro Cys Asp Thr Cys Arg
Ser Asn Ala Cys Thr Val Tyr Cys 20 25 30 His Ala Asp Ser Ala Tyr
Leu Cys Met Ser Cys Asp Ala Gln Val His 35 40 45 Ser Ala Asn Arg
Val Ala Ser Arg His Lys Arg Val Arg Val Cys Glu 50 55 60 Ser Cys
Glu Arg Ala Pro Ala Ala Phe Leu Cys Glu Ala Asp Asp Ala 65 70 75 80
Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala Asn Pro Leu 85
90 95 Ala Arg Arg His Gln Arg Val Pro Ile Leu Pro Ile Ser Gly Asn
Ser 100 105 110 Phe Ser Ser Met Thr Thr Thr His His Gln Ser Glu Lys
Thr Met Thr 115 120 125 Asp Pro Glu Lys Arg Leu Val Val Asp Gln Glu
Glu Gly Glu Glu Gly 130 135 140 Asp Lys Asp Ala Lys Glu Val Ala Ser
Trp Leu Phe Pro Asn Ser Asp 145 150 155 160 Lys Asn Asn Asn Asn Gln
Asn Asn Gly Leu Leu Phe Ser Asp Glu Tyr 165 170 175 Leu Asn Leu Val
Asp Tyr Asn Ser Ser Met Asp Tyr Lys Phe Thr Gly 180 185 190 Glu Tyr
Ser Gln His Gln Gln Asn Cys Ser Val Pro Gln Thr Ser Tyr 195 200 205
Gly Gly Asp Arg Val Val Pro Leu Lys Leu Glu Glu Ser Arg Gly His 210
215 220 Gln Cys His Asn Gln Gln Asn Phe Gln Phe Asn Ile Lys Tyr Gly
Ser 225 230 235 240 Ser Gly Thr His Tyr Asn Asp Asn Gly Ser Ile Asn
His Asn Ala Tyr 245 250 255 Ile Ser Ser Met Glu Thr Gly Val Val Pro
Glu Ser Thr Ala Cys Val 260 265 270 Thr Thr Ala Ser His Pro Arg Thr
Pro Lys Gly Thr Val Glu Gln Gln 275 280 285 Pro Asp Pro Ala Ser Gln
Met Ile Thr Val Thr Gln Leu Ser Pro Met 290 295 300 Asp Arg Glu Ala
Arg Val Leu Arg Tyr Arg Glu Lys Arg Lys Thr Arg
305 310 315 320 Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala
Tyr Ala Glu 325 330 335 Ile Arg Pro Arg Val Asn Gly Arg Phe Ala Lys
Arg Glu Ile Glu Ala 340 345 350 Glu Glu Gln Gly Phe Asn Thr Met Leu
Met Tyr Asn Thr Gly Tyr Gly 355 360 365 Ile Val Pro Ser Phe 370 (2)
INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 2493 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi)
ORIGINAL SOURCE: (A) ORGANISM: Zea mays (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 3: AAGCTTGGGC GTAGGTGTTG TCTATCGGCG AAAACACGCG
CGGTACGCCA AGAACAGCGC 60 GGCCATCTCC ATCCCAGGCA CGGTGCGCCC
GCTTTTTCGC CGTCTCGCTG AGTCACGGC 120 GGCGTCCAGC AGGTAGTTGA
GCGCCTTCCG CGGCACGAAT CGCTGCGTGC GGCCCGGAT 180 TGGTCGAGTT
GGTAGTCAGC GTCGGTGTCG AATGCCGGGA CGTCGACCAG GAAGAAGTT 240
CCGTCGCTGG GGTGGGGACG GAAGGCGTCA GGATTGTCGC AAGGGCAGAG CCCAGCCTG
300 GGGCGGGGCT ACCTCGTCGA CGCCTCGGCA CGGCGGCGGC AAAGCTGCTG
CGGGACGTG 360 CCGCCTGGGC CGCCTTCTCG GTGAAGTGGT CCTCGAAGGG
GACGAGCTCG CTGGGGTCA 420 ACCACCCCAT AGCTCGAGTC ACCGAAGAAG
GCGACGAGGA CGAGCCCGTC GCGGTGGCC 480 CGGTGTACCT CCTCGTCGTC
GGTGAGGCTG ACGCTGTAGA TATGGCCAGG CCACCACGG 540 TGGGACTTCA
CCTTGGCCCA GACCATGTCG CCGAACCGGG GGCCGCCGTT CGCCCATGC 600
ATGCCGCGTC CGGCAGCAGG AACCATGGCG CCTCCAGCGG CGGGGTCGGA CATCCTGTG
660 AGGGGAACCG AAAACCTAGA TTTGGATGCA GGTTCGATTG GTCTGGGCTT
GGGTTTGGG 720 TCCGGAGGAG GGTGGCCTGG GATCGGTGGA AGGAGGGACA
TTGTTGGTAA TTTTTATTA 780 TTTATAATAT GGAGAAATTC GAGAGACTGA
ACGATGGTGA TGTTTATTTG AGGACTATG 840 AGTATAAAGT GTAAAATAGT
ATTTTATCAA GTTTATATTC ACGTTTTTGC TGAAGATAG 900 ATAATAGTGG
AGTTGTTTTT GGCGGCTACA TAATCTTAGG CTATCTTCTC GGTCGCTCT 960
ATATCATATC TACTATCACA TTCTCTATTT TAAATTTCAC TTTGTGTAAT CTACACTA
1020 AAATAGTGTT TTACACGGTA TGTTGTACAC AGCCTTATCG TGGCGCGACG
GAGTTGGA 1080 GAGATGGTGA ACAGCTGGAT AGATATGATT TATAGGCGAT
TGGGTAGATG TGATTTGA 1140 GGTGGTTATG TAGGAGCGAT TTAGTGAGAC
ATTGTAAATA ATTAGGTTGA TGTGATCC 1200 GGATGGCTAG GTAGATATGA
TTTTAATGGA TGGTTTGGTG GACTAAGTTA TGTGGACA 1260 ATAATATGTT
TTAAATTTCT AAGAAATTGT TTGTGTTAAA TTGTATCCCA CATAGATT 1320
TTAGCCATCT CAAAGAGAGG TTTGGGTTGT TTACACAAAT AAAATATTCG TTTGCTTC
1380 CAATTTATAT GTTTTTTATT TACATGAAAA CTATATTTTT TATTCATCTA
CTCACCCA 1440 ACAGAAATTC TGGTTGAGTA GATGAAAAAA AACTACAACA
AACTCTTCCT GAAAGTGT 1500 GTGTGAAGCC GAGAAATCCT TTTCATTTCG
GTGACGGAGC CCCTTGCTGG CTGCTGCT 1560 GTGCACTCCG TTCGCCTGCC
TGCCACTACA AGCGACGGCC GACGACTCGC AAGTATCG 1620 AGGCATTTTA
AAACTGAAAA CCAAATCTAA ACCCGAATAG ACCAAATTGT TGGTTTAT 1680
GGGTTTTTGG GTTCGGATTC GGTTTCTAAA TATGCTATAT TTTAGGGTAT AGGTTCGG
1740 TCAGTTTCTA ACCTTTAAAA CCTGAATAGA CGAATAACCC GAAATATAAA
AAATCTCT 1800 ATATGTGATG ATATTATTAT ATGATTTATG AACTTATTAA
CCGAAAATAA TGATACCA 1860 CTAACGATAG TATATATATC TATGTATGCT
ATTTTTATAG TCACTTGTTG TAATAATA 1920 ACTTCCAATT AATTAATCAG
TGTATATATT TTAACAAAAG ATACTAGCCT CTCTACTA 1980 TGAGTATATT
CGGTGCACCG AATAGACCGA ACCGAAATTG TAAGTCTATT CAGGTTCG 2040
TCCTAAAATT ATTTTAAAAA TTTTGGTTCT CATATTTCAG AATCCGAAAT TTCATAAA
2100 CAAATAGACC GAACCAAATT ACGCTAATAG ACCGAATAAC TAGCGTACTC
GCAAGTCG 2160 CCCCACTAGC CTGCTGCGTG CGTAAGCGAG GACGTCACGC
GTTCTCCCTC CCGTCGAC 2220 AATACACTTG GTCTTCTAGC ACCTTCTTCC
TCTCCAAGAC TCCAATCCCC CAACCACC 2280 AACCAGCGCC AGCTCTAACG
TCACCTCTGA TTTCTCTCTC CTCTCTATTG CTAGCTGC 2340 TATTATAAGT
AGCAGCTGCA GCAGGCAGGA GCTGCACACA CCCATCCAAT TCCAGCTG 2400
GATCTTGATC CTGCACCCCG AGCCGTACAC AAGAGCTAGT CGGTAGAACT TGCAGGAG
2460 GAGCAGAACT AAGTGCAGAG AACAGGACAT ATG 2493 (2) INFORMATION FOR
SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4201 base
pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY:
linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A)
ORGANISM: Arabidopsis thaliana (B) STRAIN: Landsberg erecta (viii)
POSITION IN GENOME: (B) MAP POSITION: chromosome 5 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 4: GAATTCATGT ACCAAATCAA TACTTTTTAG
CCATAAATGA GTCAGTTTTA GTATCCACAT 60 GAATTTACCT ACCAGAGTGT
TGTAAATTAT GTTCTTTTGG GGCCACTTAC ATGGATCTC 120 TTCATTCACT
GCAGCGAGTT CTCAGACCAC CAGAAAATTT ATTCAGTGAT CTGTTTTGA 180
CATGCAACAT AAACTTATAA GCCACACAAG CAAAACAAAG ATATCCCATG TTGCATATA
240 TACGAGCTAG CATATCATAA AGAAGGAAAC TTGAAGTAGC AAAGTTTCTA
CTAAATTTC 300 TGTCAGGAAT TTTTAAAATG CAATGACAAC CACTTGGAGC
ACTATGAGTT TCAGAGCCA 360 TAGAATGTTA CTATTTGGTG TGGATTCGAG
CTAGCACGTG AAAGTGCATA AAAGTGATT 420 CCTTTTGCCA AAGGTCACTG
CACTTTTCCT CAGATAGTTT CTCACAGCCA TGGAAAGTG 480 AGAATCCGCA
TAAACGTACA ATTACAAGCT TTATATGGTC CCTCGACTCT TATTCTCTT 540
TCAGTCTTTG CAACTAAATA GGGTTTTCGT TAATCTGAAA GAAGCAAAGT ATTCGAAAC
600 ACGGAAACCT GATAAAGAAT GAAAACAAAT AAGCAATAGT GTTTTCTTGA
AAATCTCGA 660 GCAACTTTGA GGATATTGTT ACATATGATC TATTACTCGT
AACAGTTATC CGAAGGCCT 720 CACATGTGAG AGAAGTTCCA AACCGCTACA
ACAATAAACT TAATTAGAGA CTGTCAACG 780 GCAATAATAA GCAAAACTAC
TTTTTTCTTG AGCTACAAGT GAAAAGGCCA ATACACAAT 840 TACTCTTCAT
GAACTCGAAC CACGTTACAA TCTCCAAAAA ATTTCATCAC CAAAGCACT 900
AAAGCCAAAG ATGCCTCAAC TTATCCAACT TGGCAGGATA AAGATCTCCA AAAATGCTT
960 CTAAAGAACC TAGAATCTTT TCTTTAGAAT TCAATGATCA TATAACCATT TCATAACA
1020 TCTAAATGCC ATTACATTCA TCGTAAAACC AGTAAATAAC AAGAACTTGT
ATGTTAAG 1080 CCAATTACCA AGCAAAAAAA AACTTTTCAA AGTTTAAAGT
TCAAAATGGG AAAGAGAA 1140 GCGGTGTAAG CAAATATGAA AGAGGAAGAG
ATGCGAAAAG TGTATCCTAG GACCAGCA 1200 TTATACAAAA AAAAAACACT
CACTTTTCAG CTCTTAAGGC ATAGAGTGAA GGTAGCCA 1260 TGAATTTGGC
CACTAGAGCG TCCGTCAAAT CTCATTCTTT TTGGACCACA TAATGGGT 1320
CATACATTCA CTGGACCCAA AAGCGTAACT GGAGCTAGTC CTCAAACCTA GAGAGTAT
1380 TATCCTGTAG CTTCCACATA GTAAACATTA TGAGCATAAC ACCAACAAGG
CAACTCCA 1440 TACTAATGGT TATTAGTACA GGAAAACCCA CGATGCTAAA
CACATGAATG GGTCACCA 1500 TAGAGTGAAG ATGGTTAAAT TGCATCTATG
GATCATGTGG ACTAGTAAAT GAGTGTAG 1560 GAAAACTTCA CAATTACCTC
TGTGATCTTA GAAACATGTC CTGAAAATTC CATACAAG 1620 TCGTTTGTAT
TAGATTACTT CCACAGGTTG AGATCTAATA AAGCTACAAT AAATAGTA 1680
GAGTATCATC ATAAACCCAA ATTACAGAGA TGTGACAACA CTCATGAGTC ATGTTTTG
1740 ACTACTTACT ATAATGGTTA CCAAGTGCAA ATTTCTACAT ACTATATATG
ATAAATCT 1800 TTATTGCTCA TGTGGACTCC AAAATGCCTT TTAAGTTTTA
ACTTGTGCGT CAGGTAAA 1860 CTAATTTGTA GTCTCAAGAC TACTTGGCGG
ATTCGAGTTT GATCCTAGAA AATCCACC 1920 CTCTATGTTT TTCATGTCAC
TTTTCCGATA TGATTCTCAT TACCATGACT TTATGAAC 1980 GATTAAACAT
TATAACACTT TTCATCAGAA AATCCTTCGA AAGTTTCAAT TGCAAATC 2040
TCTAAATGAT GCAGATGCAT TCACAAATAA TGGAACAACA ACTATACCAT ATTCACGA
2100 TTGTCTAACC TTTGTATAGG TAGTCAACCC ATAACAGTTG GTGATGGCTC
TGACACTC 2160 AGCCTTACTC GGAGAGATAC CTGAACAGTA ATCACAAGGT
TCAGGATGAA TATTCAAC 2220 CTTAAACTTT GTATAAAGCC AAAGAGATAA
AACGAATCTA GCTTTACTTT AAATAAAA 2280 CATATGAAAA TAGTAAAAGG
TGATACGAAA AAATAGTAAC AATTTGCCTG CAACACCA 2340 GCATTATCCG
GACCACTTCC TCTTGAGAAT CTCAGTATGG CAAGTGGCAA AACCTAAG 2400
ACTTGTGAAC GGGTCCCAAC GAAGAAGTGC ATAGGAGGAG ATGTTTACAC TTTACACT
2460 ACACTTTACA CTTTACACAT AGGCCTTCCC AAAAGCTCAA CTAGCTGCAA
GAGGATCC 2520 TAACATGTAA GAGCCACTAA CGCTGTGCCA CGTGTAGGCA
CTCAGGATTC GATCTTCC 2580 TCTACTTATT CTCTCACACC AGATATAAGC
TTTATTAGCC CCTTCTTTCA GATACCAG 2640 CCCACACCAT CAAACTTACT
ACATCTGAGT TATTATGTTG AAACAAGAGA GTAACGAC 2700 AGGTAGTGGA
GAGAACAACA GGGCACGACC CTGTGACACA TGCCGGTCAA ACGCCTGC 2760
CGTGTATTGC CATGCAGATT CTGCCTACTT GTGCATGAGC TGTGATGCTC AAGTTCAC
2820 TGCCAATCGC GTTGCTTCCC GCCATAAACG TGTCCGGGTC TGCGAGTCAT
GTGAGCGT 2880 TCCGGCTGCT TTTTTGTGTG AGGCAGATGA TGCCTCTCTA
TGCACAGCCT GTGATTCA 2940 GGTTCATTCT GCAAACCCAC TTGCTAGACG
CCATCAGCGA GTTCCAATTC TACCAATT 3000 TGGAAACTCT TTCAGCTCCA
TGACCACTAC TCACCACCAA AGCGAGAAAA CAATGACC 3060 TCCAGAGAAG
AGACTGGTGG TGGATCAAGA GGAAGGTGAA GAAGGTGATA AGGATGCC 3120
GGAGGTTGCT TCGTGGCTGT TCCCTAATTC AGACAAAAAT AACAATAACC AAAACAAT
3180 GTTATTGTTT AGTGATGAGT ATCTAAACCT TGTGGATTAC AACTCGAGTA
TGGACTAC 3240 ATTCACAGGT GAATACAGTC AACACCAACA AAACTGCAGC
GTACCACAGA CGAGCTAC 3300 GGGAGATAGA GTTGTTCCGC TTAAACTTGA
AGAATCAAGG GGCCACCAGT GCCATAAC 3360 ACAGAATTTT CAGTTCAATA
TCAAATATGG CTCCTCAGGG ACTCACTACA ACGACAAT 3420 TTCCATTAAC
CATAACGTAA GGCTTTTGTA TATTTGTTAC CCCTTCAATT TAGCATCT 3480
CCATAACGCA GCAGGGTGAA TTCTTTCATC ATACACACAA ATCCACTGAT CCACTGCC
3540
CAGTTGATCT ATAGCACATA GAAATTTCAC CAGAAGTCTA TAATAAAAAC AATATATG
3600 TCCTTTTGCA TCGACTCTCT TTAGTCCTCT TACCAGGGGG ATTGAGAATG
TCTTTGTT 3660 TGTCATTAGG CATACATTTC ATCCATGGAA ACTGGTGTTG
TGCCGGAGTC AACAGCAT 3720 GTCACAACAG CTTCACACCC AAGAACGCCC
AAAGGGACAG TAGAGCAACA ACCTGACC 3780 GCAAGCCAGA TGATAACAGT
AACACAACTC AGTCCAATGG ACAGAGAAGC CAGGGTCC 3840 AGATACAGAG
AGAAGAGGAA GACAAGGAAA TTTGAGAAGA CAATAAGGTA TGCTTCGA 3900
AAGGCATATG CAGAGATAAG ACCGCGGGTC AATGGCCGGT TCGCAAAGAG AGAAATCG
3960 GCCGAGGAGC AAGGGTTCAA CACGATGCTA ATGTACAACA CAGGATATGG
GATTGTTC 4020 TCATTCTGAT ACTCCTGTGG CAAAAAGAAA AACTAGATTG
CAAGCTGTAA ATTACTTT 4080 GTTTGAGATT ATGTTAGGTT TGGTGAAATT
CTTAGCTTCA AGAAGTATTA CTACTGTT 4140 GCAAATGGGT TTGTAGTTTT
GGCTAATTAA AACTATAGTA TTCTTCTTTC TCTGCATT 4200 T 4201 (2)
INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 1101 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii)
HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A) ORGANISM: Brassic napus
(ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..1098 (xi) SEQUENCE
DESCRIPTION: SEQ ID NO: 5: ATG TTC AAA CAA GAG AGT AAC AAC ATT GGT
AGT GAA GAG AAC AAC ACC 48 Met Phe Lys Gln Glu Ser Asn Asn Ile Gly
Ser Glu Glu Asn Asn Thr 375 380 385 GGG GCG CGA GCT TGT GAC ACA TGC
GGG TCA ACC ATC TGC ACC GTG TAC 96 Gly Ala Arg Ala Cys Asp Thr Cys
Gly Ser Thr Ile Cys Thr Val Tyr 390 395 400 405 TGC CAT GCT GAC TCC
GCC TAC TTA TGC AAT AGC TGC GAT GCT CAA GTC 144 Cys His Ala Asp Ser
Ala Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 410 415 420 CAC TCT GCC
AAT CGC GTT GCT TCC CGC CAT AAA AGG GTC AGA GTG TGC 192 His Ser Ala
Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 425 430 435 GAG
TCA TGT GAG CGT GCC CCT GCT GCT TTT ATG TGT GAG GCA GAT GAT 240 Glu
Ser Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 440 445
450 GTG TCT CTA TGC ACA GCC TGT GAT TCA GAG GTT CAC TCC GCA AAC CCT
288 Val Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala Asn Pro
455 460 465 CTT GCT AGA CGC CAT CAG CGA GTT CCA GTT GTG CCG ATA ACT
GGA AAC 336 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro Ile Thr
Gly Asn 470 475 480 485 TCT TGC AGC TCC TTG GCC ACC GCT AAC CAC ACA
ACA GTG ACC GAG CCA 384 Ser Cys Ser Ser Leu Ala Thr Ala Asn His Thr
Thr Val Thr Glu Pro 490 495 500 GAG AAG AGA GTG GTG TTA GTT CAA GAG
GAT GCC AAA GAG ACG GCT TCA 432 Glu Lys Arg Val Val Leu Val Gln Glu
Asp Ala Lys Glu Thr Ala Ser 505 510 515 TGG TTG TTC CCT AAA AAC AGT
GAC AAT CAC AAC AAC AAC AAC CAG AAC 480 Trp Leu Phe Pro Lys Asn Ser
Asp Asn His Asn Asn Asn Asn Gln Asn 520 525 530 AAT GAG TTG TTG TTT
AGT GAT GAC TAT CTA GAC CTT GCT GAT TAC AAC 528 Asn Glu Leu Leu Phe
Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr Asn 535 540 545 TCG AGT ATG
GAC TAC AAG TTC ACT GGT CAA TAC AAT CAA CCT ACT CAA 576 Ser Ser Met
Asp Tyr Lys Phe Thr Gly Gln Tyr Asn Gln Pro Thr Gln 550 555 560 565
CAT AAA CAA GAC TGC ACC GTA CCA GAG AAA AAC TAC GGT GGA GAT AGA 624
His Lys Gln Asp Cys Thr Val Pro Glu Lys Asn Tyr Gly Gly Asp Arg 570
575 580 GTT GTT CCA CTC CAA CTT GAA GAA ACA AGA GGA AAC TTG CAC CAC
AAG 672 Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu His His
Lys 585 590 595 CAA CAT AAT ATC ACG TAT GGC TCC TCA GGA AGT CAC TAC
AAC AAC AAT 720 Gln His Asn Ile Thr Tyr Gly Ser Ser Gly Ser His Tyr
Asn Asn Asn 600 605 610 GGT TCC ATA AAC CAT AAC GCA TAC AAT CCA TCA
ATG GAA ACT GAC TTT 768 Gly Ser Ile Asn His Asn Ala Tyr Asn Pro Ser
Met Glu Thr Asp Phe 615 620 625 GTT CCG GAG CAG ACA GCA CCT GAC AAA
ACA GTT TCA CAT CCA AAA ACG 816 Val Pro Glu Gln Thr Ala Pro Asp Lys
Thr Val Ser His Pro Lys Thr 630 635 640 645 CAC AAA GGG AAG ATA GAG
AAA CTA CCT GAA CCT CTA ATT CAG ATT CTC 864 His Lys Gly Lys Ile Glu
Lys Leu Pro Glu Pro Leu Ile Gln Ile Leu 650 655 660 AGT CCA ATG GAC
AGA GAA GCT AGA GTC CTG AGA TAC AGA GAG AAG AAG 912 Ser Pro Met Asp
Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys 665 670 675 AAG AGA
AGA AAG TTT GAG AAG ACA ATA AGG TAT GCT TCA AGG AAG GCA 960 Lys Arg
Arg Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 680 685 690
TAT GCA GAG AGA AGA CCG AGG ATC AAT GGA CGG TTT GCA AAG ATT AGT
1008 Tyr Ala Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Ile
Ser 695 700 705 GAA ACC GAA GTA GAG GAC CAA GAG TAC AAC ACA ATG CTA
ATG TAC TAT 1056 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met
Leu Met Tyr Tyr 710 715 720 725 GAC ACA GGA TAT GGC ATT GTT CCT TCA
TTC TAT GGC CAA AAA 1098 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe
Tyr Gly Gln Lys 730 735 TAA 1101 (2) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 366 amino acids (B) TYPE:
amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 6: Met Phe Lys Gln Glu Ser Asn Asn
Ile Gly Ser Glu Glu Asn Asn Thr 1 5 10 15 Gly Ala Arg Ala Cys Asp
Thr Cys Gly Ser Thr Ile Cys Thr Val Tyr 20 25 30 Cys His Ala Asp
Ser Ala Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 35 40 45 His Ser
Ala Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val Cys 50 55 60
Glu Ser Cys Glu Arg Ala Pro Ala Ala Phe Met Cys Glu Ala Asp Asp 65
70 75 80 Val Ser Leu Cys Thr Ala Cys Asp Ser Glu Val His Ser Ala
Asn Pro 85 90 95 Leu Ala Arg Arg His Gln Arg Val Pro Val Val Pro
Ile Thr Gly Asn 100 105 110 Ser Cys Ser Ser Leu Ala Thr Ala Asn His
Thr Thr Val Thr Glu Pro 115 120 125 Glu Lys Arg Val Val Leu Val Gln
Glu Asp Ala Lys Glu Thr Ala Ser 130 135 140 Trp Leu Phe Pro Lys Asn
Ser Asp Asn His Asn Asn Asn Asn Gln Asn 145 150 155 160 Asn Glu Leu
Leu Phe Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr Asn 165 170 175 Ser
Ser Met Asp Tyr Lys Phe Thr Gly Gln Tyr Asn Gln Pro Thr Gln 180 185
190 His Lys Gln Asp Cys Thr Val Pro Glu Lys Asn Tyr Gly Gly Asp Arg
195 200 205 Val Val Pro Leu Gln Leu Glu Glu Thr Arg Gly Asn Leu His
His Lys 210 215 220 Gln His Asn Ile Thr Tyr Gly Ser Ser Gly Ser His
Tyr Asn Asn Asn 225 230 235 240 Gly Ser Ile Asn His Asn Ala Tyr Asn
Pro Ser Met Glu Thr Asp Phe 245 250 255 Val Pro Glu Gln Thr Ala Pro
Asp Lys Thr Val Ser His Pro Lys Thr 260 265 270 His Lys Gly Lys Ile
Glu Lys Leu Pro Glu Pro Leu Ile Gln Ile Leu 275 280 285 Ser Pro Met
Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys 290 295 300 Lys
Arg Arg Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser Arg Lys Ala 305 310
315 320 Tyr Ala Glu Arg Arg Pro Arg Ile Asn Gly Arg Phe Ala Lys Ile
Ser 325 330 335 Glu Thr Glu Val Glu Asp Gln Glu Tyr Asn Thr Met Leu
Met Tyr Tyr 340 345 350 Asp Thr Gly Tyr Gly Ile Val Pro Ser Phe Tyr
Gly Gln Lys 355 360 365 NFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 1101 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
DNA (genomic) (iii) HYPOTHETICAL: YES (vi) ORIGINAL SOURCE: (A)
ORGANISM: Brassica napus (ix) FEATURE: (A) NAME/KEY: CDS (B)
LOCATION:1..1098 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: ATG TTC
AAA CAA GAG AGT AAC AAC ATT TGT AAT AGA GAG AAC AAC AGA 48 Met Phe
Lys Gln Glu Ser Asn Asn Ile Cys Asn Arg Glu Asn Asn Arg 370 375 380
GGG GCA CGA GCC TGT GAC ACA TGC GGG TCA ACC ATC TGC ACC GTG TAC 96
Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr Ile Cys Thr Val Tyr 385
390 395 TGC CAT GCT GAC TCT GCC TAC TTA TGC AAT AGC TGC GAT GCT CAA
GTC 144
Cys His Ala Asp Ser Ala Tyr Leu Cys Asn Ser Cys Asp Ala Gln Val 400
405 410 CAC TCT GCC AAT CGC GTT GCT TCC CGC CAT AAA CGT GTC CGG GTC
TGC 192 His Ser Ala Asn Arg Val Ala Ser Arg His Lys Arg Val Arg Val
Cys 415 420 425 430 GAG TCA TGT GAG CGT GCC CCT GCT GCT TTT ATG TGT
GAG GCA GAT GAT 240 Glu Ser Cys Glu Arg Ala Pro Ala Ala Phe Met Cys
Glu Ala Asp Asp 435 440 445 GTG TCT CTA TGC ACA GCC TGT GAT TTA GAG
GTT CAC TCC GCA AAC CCT 288 Val Ser Leu Cys Thr Ala Cys Asp Leu Glu
Val His Ser Ala Asn Pro 450 455 460 CTT GCT AGA CGC CAT CAG CGA GTT
CCA GTT GTG CCG ATA ATT GGA AAC 336 Leu Ala Arg Arg His Gln Arg Val
Pro Val Val Pro Ile Ile Gly Asn 465 470 475 TCT TGC AGC TCC TTG GCC
ACC GCT AAC CAC ACA ACA GTG ACC GAG CCA 384 Ser Cys Ser Ser Leu Ala
Thr Ala Asn His Thr Thr Val Thr Glu Pro 480 485 490 GAG AAG AGA GTG
GTG TTA GTT CAA GAG GAT GCC AAA GAG ACG GCT TCA 432 Glu Lys Arg Val
Val Leu Val Gln Glu Asp Ala Lys Glu Thr Ala Ser 495 500 505 510 TGG
TTG TTC CCT AAA AAC AGT GAC TAT CAC AAC AAC AAC AAC AAC CAG 480 Trp
Leu Phe Pro Lys Asn Ser Asp Tyr His Asn Asn Asn Asn Asn Gln 515 520
525 AAC AAT GAG TTG TTG TTT AGT GAT GAC TAC CTA GAC CTT GCT GAT TAC
528 Asn Asn Glu Leu Leu Phe Ser Asp Asp Tyr Leu Asp Leu Ala Asp Tyr
530 535 540 AAC TCC AGT ATG GAC TAC AAG TTC ACC AGT CAA TAC AAT CAA
CCT CGA 576 Asn Ser Ser Met Asp Tyr Lys Phe Thr Ser Gln Tyr Asn Gln
Pro Arg 545 550 555 CAT AAA CAA GAC TGC ATC GTA CCA GAG AAA AAC TAC
AGT GGA GAT AGA 624 His Lys Gln Asp Cys Ile Val Pro Glu Lys Asn Tyr
Ser Gly Asp Arg 560 565 570 GTT GTT CCG CTC CAA CTT GAA GAA ACA AGA
GGA AAC TTG CGG AAC AAG 672 Val Val Pro Leu Gln Leu Glu Glu Thr Arg
Gly Asn Leu Arg Asn Lys 575 580 585 590 CAA CAG AAT ATC ACA TAT GGC
TCC TCA GGA AGC CAA TAC AAC AAC AAC 720 Gln Gln Asn Ile Thr Tyr Gly
Ser Ser Gly Ser Gln Tyr Asn Asn Asn 595 600 605 GGT TCC ATT AAC CAT
AAC GCA TAC AAT CCA TCA ATG GAA ACT GAC TTT 768 Gly Ser Ile Asn His
Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp Phe 610 615 620 GTG CCG GAG
CAG ACA GCA CCT GAC ACA ACA GTT TCA CAT CCA AAA ACG 816 Val Pro Glu
Gln Thr Ala Pro Asp Thr Thr Val Ser His Pro Lys Thr 625 630 635 CAC
AAA GGG AAG ACA GCA CAA CTA CCT GAA CCT CTA ATT CAG ATT CTC 864 His
Lys Gly Lys Thr Ala Gln Leu Pro Glu Pro Leu Ile Gln Ile Leu 640 645
650 AGT CCA ATG GAC AGA GAA GCT AGA GTC CTG AGA TAC AGA GAG AAG AAG
912 Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr Arg Glu Lys Lys
655 660 665 670 AAG AGA AGA AAG TTT GAG AAG ACA ATA AGG TAT GCT TCA
AGG AAG GCA 960 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg Tyr Ala Ser
Arg Lys Ala 675 680 685 TAT GCA GAG AGA AGA CCG AGG ATA AAT GGA CGG
TTT GCA AAG ATG AGT 1008 Tyr Ala Glu Arg Arg Pro Arg Ile Asn Gly
Arg Phe Ala Lys Met Ser 690 695 700 GAA ACC GAA GTA GAG GAC CAA GAG
TAC AAC ACA ATG CTA ATG TAC TGC 1056 Glu Thr Glu Val Glu Asp Gln
Glu Tyr Asn Thr Met Leu Met Tyr Cys 705 710 715 GAC ACA GGA TAT GGC
ATT GTT CCT TCA TTC TAT GGC CAA AAA 1098 Asp Thr Gly Tyr Gly Ile
Val Pro Ser Phe Tyr Gly Gln Lys 720 725 730 TAA 1101 (2)
INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 366 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
8: Met Phe Lys Gln Glu Ser Asn Asn Ile Cys Asn Arg Glu Asn Asn Arg
1 5 10 15 Gly Ala Arg Ala Cys Asp Thr Cys Gly Ser Thr Ile Cys Thr
Val Tyr 20 25 30 Cys His Ala Asp Ser Ala Tyr Leu Cys Asn Ser Cys
Asp Ala Gln Val 35 40 45 His Ser Ala Asn Arg Val Ala Ser Arg His
Lys Arg Val Arg Val Cys 50 55 60 Glu Ser Cys Glu Arg Ala Pro Ala
Ala Phe Met Cys Glu Ala Asp Asp 65 70 75 80 Val Ser Leu Cys Thr Ala
Cys Asp Leu Glu Val His Ser Ala Asn Pro 85 90 95 Leu Ala Arg Arg
His Gln Arg Val Pro Val Val Pro Ile Ile Gly Asn 100 105 110 Ser Cys
Ser Ser Leu Ala Thr Ala Asn His Thr Thr Val Thr Glu Pro 115 120 125
Glu Lys Arg Val Val Leu Val Gln Glu Asp Ala Lys Glu Thr Ala Ser 130
135 140 Trp Leu Phe Pro Lys Asn Ser Asp Tyr His Asn Asn Asn Asn Asn
Gln 145 150 155 160 Asn Asn Glu Leu Leu Phe Ser Asp Asp Tyr Leu Asp
Leu Ala Asp Tyr 165 170 175 Asn Ser Ser Met Asp Tyr Lys Phe Thr Ser
Gln Tyr Asn Gln Pro Arg 180 185 190 His Lys Gln Asp Cys Ile Val Pro
Glu Lys Asn Tyr Ser Gly Asp Arg 195 200 205 Val Val Pro Leu Gln Leu
Glu Glu Thr Arg Gly Asn Leu Arg Asn Lys 210 215 220 Gln Gln Asn Ile
Thr Tyr Gly Ser Ser Gly Ser Gln Tyr Asn Asn Asn 225 230 235 240 Gly
Ser Ile Asn His Asn Ala Tyr Asn Pro Ser Met Glu Thr Asp Phe 245 250
255 Val Pro Glu Gln Thr Ala Pro Asp Thr Thr Val Ser His Pro Lys Thr
260 265 270 His Lys Gly Lys Thr Ala Gln Leu Pro Glu Pro Leu Ile Gln
Ile Leu 275 280 285 Ser Pro Met Asp Arg Glu Ala Arg Val Leu Arg Tyr
Arg Glu Lys Lys 290 295 300 Lys Arg Arg Lys Phe Glu Lys Thr Ile Arg
Tyr Ala Ser Arg Lys Ala 305 310 315 320 Tyr Ala Glu Arg Arg Pro Arg
Ile Asn Gly Arg Phe Ala Lys Met Ser 325 330 335 Glu Thr Glu Val Glu
Asp Gln Glu Tyr Asn Thr Met Leu Met Tyr Cys 340 345 350 Asp Thr Gly
Tyr Gly Ile Val Pro Ser Phe Tyr Gly Gln Lys 355 360 365 (2)
INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
9: TCCTGCTCGC TTCGCTACTT 20 (2) INFORMATION FOR SEQ ID NO: 10: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 10: GCGATGCTGT CGGAATGGAC 20 (2)
INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
11: AAGTACTCTC GGTAGCCAAG 20 (2) INFORMATION FOR SEQ ID NO: 12: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for pcr" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 12: GTGTGGTCGC CATGATCGCG 20 (2)
INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: CTGGGAAGTG AATGGAGACA TA
22 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
14: AGGAGTCGCA TAAGGGAGAG 20 (2) INFORMATION FOR SEQ ID NO: 15: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 15: ACGTCGGATG CTCACTATAG GGATC 25
(2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
16: GTGATAAACT ACCGCATTAA AGC 23 (2) INFORMATION FOR SEQ ID NO: 17:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 17: ACGTCGGATG ACTTTAATTT ATTCACTA
28 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
18: GACGTGGATG CTCACTAAAG GGATC 25 (2) INFORMATION FOR SEQ ID NO:
19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: AGAGCCTTCA ACCCAGTCAG 20
(2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS:
single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid
(A) DESCRIPTION: /desc = "primer used for PCR" (vi) ORIGINAL
SOURCE: (A) ORGANISM: yeast (xi) SEQUENCE DESCRIPTION: SEQ ID NO:
20: ACGTCGGATG CCGATCTCAA GATTA 25 (2) INFORMATION FOR SEQ ID NO:
21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
GCTCCCACAC CATCAAACTT ACTAC 25 (2) INFORMATION FOR SEQ ID NO: 22:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: Arabidopsis
thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: CTCCTCGGCT
TCGATTTCTC 20 (2) INFORMATION FOR SEQ ID NO: 23: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE:
other nucleic acid (A) DESCRIPTION: /desc = "primer used for PCR"
(vi) ORIGINAL SOURCE: (A) ORGANISM: designed primer not derived
from any organism (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:
GACTCGAGTC GACATCG 17 (2) INFORMATION FOR SEQ ID NO: 24: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
TGCAGATTCT GCCTACTTGT GC 22 (2) INFORMATION FOR SEQ ID NO: 25: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
TGATTCTGCC TACTTGTGCT C 21 (2) INFORMATION FOR SEQ ID NO: 26: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
synthesised for PCR" (vi) ORIGINAL SOURCE:
(A) ORGANISM: Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ
ID NO: 26: GCTTGGTTTG CCTCTTCATC 20 (2) INFORMATION FOR SEQ ID NO:
27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B)
TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"primer synthesised for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
GGTCCCAACG AAGAAGTGC 19 (2) INFORMATION FOR SEQ ID NO: 28: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"synthesised primer for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM:
Arabidopsis thaliana (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
CAGGGAGGCG TGAAAGTGT 19 (2) INFORMATION FOR SEQ ID NO: 29: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "adapter
synthesised to (vi) ORIGINAL SOURCE: (A) ORGANISM: designed
sequence not derived from any organism (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 29: TACAAGCTTG 10 (2) INFORMATION FOR SEQ ID NO: 30: (i)
SEQUENCE CHARACTERISTICS: (A) LENGTH: 12 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc =
"oligonucleotide (vi) ORIGINAL SOURCE: (A) ORGANISM: designed
sequence, not derived from any organism (xi) SEQUENCE DESCRIPTION:
SEQ ID NO: 30: TATCGATAGT AC 12 (2) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE:
nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii)
MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer
used for PCR" (vi) ORIGINAL SOURCE: (A) ORGANISM: yeast (xi)
SEQUENCE DESCRIPTION: SEQ ID NO: 31: TTCAAGCTCT ACGCCGGA 18 (2)
INFORMATION FOR SEQ ID NO: 32: (i) SEQUENCE CHARACTERISTICS: (A)
LENGTH: 24 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein (ix) FEATURE: (A) NAME/KEY:
Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /label= Xaa
/note= Xaa at positions 2, 3, 5-20, 22 and 23 can be any amino acid
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32: Cys Xaa Xaa Cys Xaa Xaa
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa
Cys Xaa Xaa Cys 20 (2) INFORMATION FOR SEQ ID NO: 33: (i) SEQUENCE
CHARACTERISTICS: (A) LENGTH: 25 amino acids (B) TYPE: amino acid
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (ix) FEATURE: (A)
NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION:
/label= Xaa /note= Xaa at positions 2, 3, 5-21, 23 and 24 can be
any amino acid (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33: Cys Xaa
Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15
Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Cys 20 25
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