Mycobacterial rpoB sequences

Abstract

This invention provides polynucleotide probes, sequences and methods for speciating and phenotyping organisms, for example, using probes based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application derives priority from U.S. Ser. No. 60/080,616, filed Apr. 3, 1998, and incorporated by reference. Applications U.S. Ser. No. 08/797,812, filed Feb. 7, 1997, now U.S. Pat. No. 6,228,575; U.S. Ser. No. 60/011,339, filed Feb. Feb. 8, 1996; U.S. Ser. No. 60/012,631, filed Mar. 1, 1996; U.S. Ser. No. 08/629,031, filed Apr. 8, 1996, now abandoned; and 60/017,765, filed 15 May 15, 1996 are directed to related subject matter. These applications are specifically incorporated by reference in their entirety for all purposes.

BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] This invention is directed to polymorphisms in rpoB genes of mycobacteria and use of the same in the identification and characterization of microorganisms.

[0005] 2. Background of the Invention

[0006] Multidrug resistance and human immunodeficiency virus (HIV-1) infections are factors which have had a profound impact on the tuberculosis problem. An increase in the frequency of Mycobacterium tuberculosis strains resistant to one or more anti-mycobacterial agents has been reported, Block, et al., (1994) JAMA 271:665-671. Immunocompromised HIV-1 infected patients not infected with M. tuberculosis are frequently infected with M. avium complex (MAC) or M. avium-M. intracellulare (MAI) complex. These mycobacteria species are often resistant to the drugs used to treat M. tuberculosis. These factors have re-emphasized the importance for the accurate determination of drug sensitivities and mycobacteria species identification.

[0007] In HIV-1 infected patients, the correct diagnosis of the mycobacterial disease is essential since treatment of M. tuberculosis infections differs from that called for by other mycobacteria infections, Hoffner, S. E. (1994) Eur. J. Clin. Microbiol. Inf. Dis. 13:937-941. Non-tuberculosis mycobacteria commonly associated with HIV-1 infections include M. kansasii, M. xenopi, M. fortuitum, M. avium and M. intracellulare, Wolinsky, E., (1992) Clin. Infect. Dis. 15:1-12, Shafer, R. W. and Sierra, M. F. 1992 Clin. Infect. Dis. 15:161-162. Additionally, 13% of new cases (HIV-1 infected and non-infected) of M. tuberculosis are resistant to one of the primary anti-tuberculosis drugs (isoniazid [INH], rifampin [RIF], streptomycin [STR], ethambutol [EMB] and pyrazinamide [PZA] and 3.2% are resistant to both RIF and INH, Block, et al., JAMA 271:665-671, (1994). Consequently, mycobacterial species identification and the determination of drug resistance have become central concerns during the diagnosis of mycobacterial diseases.

[0008] Methods used to detect, and to identify Mycobacterium species vary considerably. For detection of Mycobacterium tuberculosis, microscopic examination of acid-fast stained smears and cultures are still the methods of choice in most microbiological clinical laboratories. However, culture of clinical samples is hampered by the slow growth of mycobacteria. A mean time of four weeks is required before sufficient growth is obtained to enable detection and possible identification. Recently, two more rapid methods for culture have been developed involving a radiometric, Stager, C. E. et al., (1991) J. Clin. Microbiol. 29:154-157, and a biphasic (broth/agar) system Sewell, et al., (1993) J. Clin. Microbiol. 29:2689-2472. Once grown, cultured mycobacteria can be analyzed by lipid composition, the use of species specific antibodies, species specific DNA or RNA probes and PCR-based sequence analysis of 16S rRNA gene (Schirm, et al. (1995) J. Clin. Microbiol. 33:3221-3224; Kox, et al. (1995) J. Clin. Microbiol. 33:3225-3233) and IS6110 specific repetitive sequence analysis (For a review see, e.g., Small et al., P. M. and van Embden, J. D. A. (1994) Am. Society for Microbiology, pp. 569-582). The analysis of 16S rRNA sequences (RNA and DNA) has been the most informative molecular approach to identify Mycobacteria species (Jonas, et al., J. Clin. Microbiol. 31:2410-2416 (1993)). However, to obtain drug sensitivity information for the same isolate, additional protocols (culture) or alternative gene analysis is necessary.

[0009] To determine drug sensitivity information, culture methods are still the protocols of choice. Mycobacteria are judged to be resistant to particular drugs by use of either the standard proportional plate method or minimal inhibitory concentration (MIC) method. However, given the inherent lengthy times required by culture methods, approaches to determine drug sensitivity based on molecular genetics have been recently developed.

[0010] Because resistance to RIF in E. coli strains was observed to arise as a result of mutations in the rpoB gene, Telenti, et al., id., identified a 69 base pair (bp) region of the M. tuberculosis rpoB gene as the locus where RIF resistant mutations were focused. Kapur, et al., (1995) Arch. Pathol. Lab. Med. 119:131-138, identified additional novel mutations in the M. tuberculosis rpoB gene which extended this core region to 81 bp. In a detailed review on antimicrobial agent resistance in mycobacteria, Musser (Clin. Microbiol. Rev., 8:496-514 (1995)), summarized all the characterized mutations and their relative frequency of occurrence in this 81 bp region of rpoB. Missense mutations comprise 88% of all known mutations while insertions (3 or 6 bp) and deletions (3, 6 and 9 bp) account for 4% and 8% of the remaining mutations, respectively. Approximately 90% of all RIF resistant tuberculosis isolates have been shown to have mutations in this 81 bp region. The remaining 10% are thought possibly to involve genes other than rpoB.

[0011] For the above reasons, it would be desirable to have simpler methods which identify and characterize microorganisms, such as Mycobacteria, both at the phenotypic and genotypic level. This invention fulfills that and related needs.

SUMMARY OF THE INVENTION

[0012] In one aspect, the invention provides isolated nucleic acids comprising at least 25, 50, 75, 100, or 200 contiguous bases from an rpoB sequence shown in Table 1 (SEQ ID NOS: 1-181). Some nucleic acid comprise a complete sequence shown in Table 1.

[0013] The invention further provides a set of probes perfectly complementary to and spanning such nucleic acids, preferably spanning one of the complete sequences shown in Table 1 (SEQ ID NOS: 1-181).

[0014] The invention further provides methods of classifying mycobacteria. Some such methods entail providing a sample comprising a mycobacterial rpoB target nucleic acid from a mycobacteria, determining the sequence of a segment of at least 50 contiguous bases from the target nucleic acid; comparing the determined sequence to at least one sequence shown in Table 1; and classifying the mycobacteria from the extent of similarity of the compared sequences. Preferably, at least 100 or 200 contiguous bases are determined from the target nucleic acid. Preferably, the determined sequence is compared with a plurality of sequences from Table 1, for example, 10, 20, 50 or all of the sequence from Table 1 (SEQ ID NOS: 1-181).

[0015] In other methods of classification, the identity of one or more bases in the target sequence at one or more positions corresponding to one or more of the highlighted positions in a sequence shown in Table 1 is determined. The identity of the one or more bases characterizing the species of mycobacteria that is present in the sample. In some methods, the identity of at least 10 bases in the target nucleic acid at positions corresponding to highlighted positions in a sequence shown in Table 1 is determined. In some methods, the identity of at least 20 bases in the target sequence at highlighted positions shown in Table 1 are identified. In some methods, at least 20 determined bases are compared with 20 bases occupying corresponding positions in each of at least ten sequences from Table 1.

[0016] In another aspect, the invention provides sequence-specific polynucleotide probes or primers that hybridizes to a segment of a mycobacterial rpoB sequence shown in Table 1 or its complement without hybridizing to the M. tuberculosis sequence designated ATCC9-Mtb in Table 1 or its complement, the segment including a highlighted nucleotide position shown in Table 1. In some such probes, a central position of the probe aligns with a highlighted nucleotide position shown in Table 1. In some such primers, the 3' end of the primer aligns with a highlighted nucleotide position shown in Table 1. Some probes and primers are between 10 and 50 bases long.

[0017] In another aspect, the invention provides a computer-readable storage medium for storing data for access by an application program being executed on a data processing system. Such a system comprises a data structure stored in the computer-readable storage medium. The data structure includes information resident in a database used by the application program and includes a plurality of records, each record comprising information identifying a polymorphism or sequence shown in Table 1. Some records have a field identifying a base occupying a polymorphic site and a field identifying location of the polymorphic site. Some records record a contiguous segment of at least 50, 100, or 200 bases from an rpoB sequence shown in Table 1. Some storage medium comprise at least ten records each recording a contiguous segment of at least 50 bases from at least ten rpoB sequences shown in Table 1.

BRIEF DESCRIPTION OF THE DRAWINGS

[0018] FIG. 1: Computer that may be utilized to execute software embodiments of the present invention.

[0019] FIG. 2: A system block diagram of a typical computer system that may be used to execute software embodiments of the invention.

DEFINITIONS

[0020] A polynucleotide can be DNA or RNA, and single- or double-stranded. Polynucleotide can be naturally occurring or synthetic, and can be of any length. Preferred polynucleotide probes of the invention include contiguous segments of DNA, or their complements including any of the highlighted bases shown in Table 1. The segments are usually between 5 and 100 bases, and often between 5-10, 5-20, 10-20, 10-50, 20-50 or 20-100 bases. The highlighted site can occur within any position of the segment. Preferred polynucleotide probes are capable of binding in a base-specific manner to a complementary strand of nucleic acid. Such probes include peptide nucleic acids, as described in Nielsen et al., Science 254, 1497-1500 (1991), and probes having nonnaturally occurring bases.

[0021] The term primer refers to a single-stranded polynucleotide capable of acting as a point of initiation of template-directed DNA synthesis under appropriate conditions (i.e., in the presence of four different nucleoside triphosphates and an agent for polymerization, such as, DNA or RNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature. The appropriate length of a primer depends on the intended use of the primer but typically ranges from 15 to 30 nucleotides. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template. The term primer site refers to the area of the target DNA to which a primer hybridizes. The term primer pair means a set of primers including a 5' upstream primer that hybridizes with the 5' end of the DNA sequence to be amplified and a 3', downstream primer that hybridizes with the complement of the 3' end of the sequence to be amplified.

[0022] A cDNA or cRNA is derived from an RNA if it produced by a process in which the RNA serves as a template for production of the cDNA or cRNA.

[0023] Hybridizations are usually performed under stringent conditions, for example, at a salt concentration of no more than 1 M and a temperature of at least 25.degree. C. For example, conditions of 5.times.SSPE (750 mM NaCl, 50 mM Na Phosphate, 5 mM EDTA, pH 7.4) and a temperature of 25-30.degree. C. are suitable for allele-specific probe hybridizations.

[0024] An isolated nucleic acid means an object species invention that is the predominant species present (i.e., on a molar basis it is more abundant than any other individual species in the composition). Preferably, an isolated nucleic acid comprises at least about 50, 80 or 90 percent (on a molar basis) of all macromolecular species present. Most preferably, the object species is purified to essential homogeneity (contaminant species cannot be detected in the composition by conventional detection methods).

[0025] For sequence comparison and homology determination, typically one sequence acts as a reference sequence to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters.

[0026] Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by visual inspection (see generally, Ausubel et al., infra).

[0027] One example of algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is described in Altschul et al., J. Mol. Biol. 215:403-410 (1990). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=-4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff (1989) Proc. Natl. Acad. Sci. USA 89:10915).

[0028] In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin & Altschul (1993) Proc. Nat'l. Acad. Sci. USA 90:5873-5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001.

[0029] The term "target nucleic acid" refers to a nucleic acid (often derived from a biological sample), to which the probe nucleic acid is designed to specifically hybridize. It is the presence or expression level of the target nucleic acid that is to be detected or quantified. The target nucleic acid has a sequence that is complementary to the nucleic acid sequence of the corresponding probe directed to the target. The term target nucleic acid may refer to the specific subsequence of a larger nucleic acid to which the probe is directed or to the overall sequence (e.g. gene or mRNA) whose expression level it is desired to detect. The difference in usage will be apparent from context.

[0030] "Subsequence" refers to a sequence of nucleic acids that comprise a part of a longer sequence of nucleic acids.

DETAILED DESCRIPTION

I. Mycobacterial Sequences of rpoB Genes

[0031] Table 1 shows a comparison of a substantial collection of mycobacterial strains of an about 700-nucleotide conserved region of an rpoB gene. The sequences shown in Table 1 are identified as follows: SEQ ID NOS: 1-56, respectively, are shown on pages 21, 25, 29, 33, 37, 41, 45, 49, 53, 57, 61 and 65; SEQ ID NOS: 57-112, respectively, are shown on pages 22, 26, 30, 34, 38, 42, 46, 50, 54, 58, 62 and 65; SEQ ID NOS: 113-168, respectively, are shown on pages 23, 27, 31, 35, 39, 43, 47, 51, 55, 59, 63 and 66; SEQ ID NOS: 169-181, respectively, are shown on pages 24, 28, 32, 36, 40, 44, 52, 56, 60, 64 and 68. The first sequence, designated as a reference sequence, is from M. tuberculosis. Nucleotides are numbered consecutively starting from the first nucleotide of the reference sequences. Other sequences are from other strains of mycobacteria. For example, the sequences designated ATCC-av, M29, M30 . . . M104 are from M. avium. Sequences designated from ATT-chelnew, M11, M13, and M17 are from M. chelonae. Sequences designated ATCC-for, M53, M55, M56, and M74 are from M. fortuitum, and so forth. Complete correspondence between strain designations and strain types is shown in Table 2. Nucleotides in a mycobacterial sequence are accorded the same number as the corresponding position of the reference sequence when the two are maximally aligned. Differences between a sequence and the reference sequences are shown in highlighted type. Many of the highlighted positions are common to all tested members of a species. Other highlighted positions vary among different isolates in a species. Both types of variation can be useful in speciation analysis.

II. Analysis of Species Variations

[0032] A. Preparation of Samples

[0033] An rpoB sequence is isolated from a sample of an unknown mycobacteria being tested. Nucleic acids can be isolated from myobacteria by standard methods as described in WO 97/29212 (incorporated by reference in its entirety for all purposes). The rpoB sequences to be analyzed can then be isolated and amplified by means of PCR. See generally PCR Technology. Principles and Applications for DNA Amplification (ed. H. A. Erlich, Freeman Press, NY, N.Y., 1992); PCR Protocols: A Guide to Methods and Applications (eds. Innis, et al., Academic Press, San Diego, Calif., 1990); Mattila et al., Nucleic Acids Res. 19, 4967 (1991); Eckert et al., PCR Methods and Applications 1, 17 (1991); PCR (eds. McPherson et al., IRL Press, Oxford); and U.S. Pat. No. 4,683,202 (each of which is incorporated by reference for all purposes). Primers for PCR preferably flank the regions of interest rpoB genes, although primers to internal sites can be used if it is intended to analyze only certain sites of potential species variation. Exemplary primers are described in WO 97/29212. If necessary, additional sequences flanking the sequences shown in Table 1 can be determined using probes based on the sequences in Table 1 to isolate full-length rpoB sequences from the appropriate mycobacterial species.

[0034] B. Detection of Species-Specific Variations in Target DNA

[0035] 1. Sequence-Specific Probes

[0036] The design and use of sequence-specific probes for analyzing polymorphisms is described by e.g., Saiki et al., Nature 324, 163-166 (1986); Dattagupta, EP 235,726, Saiki, WO 89/11548. Sequence-specific probes can be designed that hybridize to a segment of target DNA in one isolate of mycobacteria that do not isolate to a corresponding isolate in another due to the presence of allelic or species variations in the respective segments from the two sequences. Hybridization conditions should be sufficiently stringent that there is a significant difference in hybridization intensity between alleles, and preferably an essentially binary response, whereby a probe hybridizes to only one of the sequences. Some probes are designed to hybridize to a segment of target DNA such that the site of potential sequence variation aligns with a central position (e.g., in a 15 mer at the 7 position; in a 16 mer, at either the 8 or 9 position) of the probe. This design of probe achieves good discrimination in hybridization between different allelic and species variants.

[0037] Sequence-specific probes are often used in pairs, one member of a pair showing a perfect match to a reference form of a target sequence and the other member showing a perfect match to a variant form. Several pairs of probes can then be immobilized on the same support for simultaneous analysis of multiple potential variations within the same target sequence.

[0038] 2. Tiling Arrays

[0039] The bases occupying sites of potential variation can also be identified by hybridization to nucleic acid arrays, some example of which are described by WO 95/11995 (incorporated by reference in its entirety for all purposes). Such arrays contain a series of overlapping probes spanning a reference sequence. Any of the rpoB sequences shown in Table 1, or contiguous segments of, for example, at least 25, 50, 100 or 200 bases thereof, can serve as a reference sequence. WO 95/11995 also describes subarrays that are optimized for detection of a variant forms of a precharacterized polymorphism. Such a subarray contains probes designed to be complementary to a second reference sequence, which is a variant of the first reference sequence. The inclusion of a second group (or further groups) can be particular useful for analyzing short subsequences of the primary reference sequence in which multiple mutations are expected to occur within a short distance commensurate with the length of the probes (i.e., two or more mutations within 9 to 21 bases).

[0040] 3. Sequence-Specific Primers

[0041] A sequence-specific primer hybridizes to a site on target DNA overlapping a polymorphism and only primes amplification of a variant form to which the primer exhibits perfect complementarily. See Gibbs, Nucleic Acid Res. 17, 2427-2448 (1989). This primer is used in conjunction with a second primer which hybridizes at a distal site. Amplification proceeds from the two primers leading to a detectable product signifying the particular variant form is present. A control is usually performed with a second pair of primers, one of which shows a single base mismatch at the site of variation and the other of which exhibits perfect complementarily to a distal site. The single-base mismatch prevents amplification and no detectable product is formed. The method works best when the mismatch is included in the 3'-most position of the primer aligned with the point of variation because this position is most destabilizing to elongation from the primer. See, e.g., WO 93/22456.

[0042] 4. Direct-Sequencing

[0043] The direct analysis of mycobacterial sequences can be accomplished using either the dideoxy chain termination method or the Maxam Gilbert method (see Sambrook et al., Molecular Cloning, A Laboratory Manual (2nd Ed., CSHP, New York 1989); Zyskind et al., Recombinant DNA Laboratory Manual, (Acad. Press, 1988)).

III. Methods of Use

[0044] The sequences and polymorphisms shown in Table 1 are useful for identifying the presence of myobacteria in samples, and optionally, classifying the mycobacteria. The sample can be obtained from a patient or from a biological source, such as a food product.

[0045] The sequences shown in Table 1 can be used for design of sequence-specific probes or primers encompassing polymorphic sites as described above. These probes or primers can then be used to determine the base occupying a corresponding position in an rpoB sequence from an isolate in a sample under test. A base in one sequence corresponds with a base in another when the two bases occupy the same position when the two sequences are maximally aligned by one of the criteria described in Definitions.

[0046] Alternatively, the sequences shown in Table 1 can be used for design of tiling arrays in which one or more of the sequences serves as a reference sequence. At least one set of overlapping probes is designed spanning a segment of the reference sequence, as described in WO95/11995 or EP 717,113. Target sequences from samples under test can be hybridized to such arrays, optionally in combination with controls of known rpoB sequences. The hybridization pattern of a target sequence to such an array can be analyzed to determine the identity of bases at which the target sequence differs from the reference sequence, as described in WO 95/11995.

[0047] One or more of the above methods, or direct sequencing, can be used to identify the base occupying at least one and usually several (e.g., 5, 10, 15, 25, 50 or 100) sites of potential variation between the 16S RNA and/or rpoB gene in an unknown mycobacteria relative to bases occupying corresponding sites in one or more known strains of mycobacteria, such as those shown in Table 1. This analysis results in a profile of bases occupying particular sites that characterizes the mycobacterial strain under test. The profile is compared with the corresponding profiles of different mycobacterial isolates shown in e.g., Table 1. In general, the unknown mycobacterium isolate is characterized as being from the same mycobacterial species as the precharacterized isolate with which it shares the greatest similarity in base profile.

[0048] In some methods, the sequence of a contiguous segment of the rpoB target nucleic acid is determined in a sample under test for comparison with one or more of the sequences shown in Table 1. The mycobacteria is classified by the extent of similarity. For example, if a target nucleic acid shows greater sequence identity to rpoB sequences from one species than any other, the sample from which the target was obtained is typically classified as arising from that species.

[0049] Alternatively, an array of tiled probes based on a reference sequence shown in Table 1 can be used for identifying and characterizing mycobacterial sequences based on comparison of hybridization patterns. Such an array is hybridized to a 16S RNA or rpoB target sequence from a sample, and the hybridization pattern compared with the hybridization pattern of one or more control sequences. The hybridization patterns of control sequences can be historic controls, stored, for example, in a computer database, or can be contemporaneous controls performed at or near the same time as the hybridization to the target sequence. Optionally, hybridization of target and reference sequence can be performed simultaneously using different labels.

[0050] Method of classifying unknown mycobacterial isolate by matching the hybridization pattern of a target sequence with those of control sequences from characterized species are described in more detail in WO 97/29212 (incorporated by reference in its entirety for all purposes). In an idealized case, the detection of a particular hybridization pattern in an isolate characterizes that isolate as belonging to a particular species. This can occur when the hybridization pattern detected in the isolate is uniquely associated with a specific species. More frequently however, such an unique one-to-one correspondence is not present. Instead, the hybridization pattern observed in an isolate does not bear a unique correspondence with a previously characterized species. However, the hybridization pattern detected is associated with a probability of the organism being screened belonging to a particular species (or not) or carrying a particular phenotypic trait (or not). As a result, analysis of an increasing number of polymorphic sites in an isolate, allows one to classify the isolated with an increasing level of confidence. Algorithms can be used to derive such composite probabilities from the comparison of multiple polymorphic forms between an isolate and references. Typically, the mathematical algorithm makes a call of the identity of the species and assign a confidence level to that call. One can determine the confidence level (>90%, >95% etc.) that one desires and the algorithm will analyze the hybridization pattern and either provide an identification or not. Occasionally, the call is that the sample may be one of two, three or more species, in which case a specific identification is not be possible. However, one of the strengths of this technique is that the rapid screening made possible by the chip-based hybridization allows one to continuously expand a database of patterns ultimately to enable the identification of species previously unidentifiable due to lack of sufficient information.

IV. Modified Polypeptides and Gene Sequences

[0051] The invention further provides variant forms of nucleic acids and corresponding proteins. The nucleic acids comprise one of the sequences described in Table 1. Some nucleic acid encode full-length variant forms of proteins. Variant proteins have the prototypical amino acid sequences of encoded by nucleic acid sequence shown in Table 1 (read so as to be in-frame with the full-length coding sequence of which it is a component).

[0052] Variant genes can be expressed in an expression vector in which a variant gene is operably linked to a native or other promoter. Usually, the promoter is a eukaryotic promoter for expression in a mammalian cell. The transcription regulation sequences typically include a heterologous promoter and optionally an enhancer which is recognized by the host. The selection of an appropriate promoter, for example trp, lac, phage promoters, glycolytic enzyme promoters and tRNA promoters, depends on the host selected. Commercially available expression vectors can be used. Vectors can include host-recognized replication systems, amplifiable genes, selectable markers, host sequences useful for insertion into the host genome, and the like.

[0053] The means of introducing the expression construct into a host cell varies depending upon the particular construction and the target host. Suitable means include fusion, conjugation, transfection, transduction, electroporation or injection, as described in Sambrook, supra. A wide variety of host cells can be employed for expression of the variant gene, both prokaryotic and eukaryotic. Suitable host cells include bacteria such as E. coli, yeast, filamentous fungi, insect cells, mammalian cells, typically immortalized, e.g., mouse, CHO, human and monkey cell lines and derivatives thereof. Preferred host cells are able to process the variant gene product to produce an appropriate mature polypeptide. Processing includes glycosylation, ubiquitination, disulfide bond formation, general post-translational modification, and the like.

[0054] The protein may be isolated by conventional means of protein biochemistry and purification to obtain a substantially pure product, i.e., 80, 95 or 99% free of cell component contaminants, as described in Jacoby, Methods in Enzymology Volume 104, Academic Press, New York (1984); Scopes, Protein Purification, Principles and Practice, 2nd Edition, Springer-Verlag, New York (1987); and Deutscher (ed), Guide to Protein Purification, Methods in Enzymology, Vol. 182 (1990). If the protein is secreted, it can be isolated from the supernatant in which the host cell is grown. If not secreted, the protein can be isolated from a lysate of the host cells.

[0055] In addition to substantially full-length polypeptides expressed by variant genes, the present invention includes biologically active fragments of the polypeptides, or analogs thereof, including organic molecules which simulate the interactions of the peptides. Biologically active fragments include any portion of the full-length polypeptide which confers a biological function on the variant gene product, including ligand binding, and antibody binding. Ligand binding includes binding by nucleic acids, proteins or polypeptides, small biologically active molecules, or large cellular structures.

[0056] Polyclonal and/or monoclonal antibodies that specifically bind to variant gene products but not to corresponding prototypical gene products are also provided. Antibodies can be made by injecting mice or other animals with the variant gene product or synthetic peptide fragments thereof. Monoclonal antibodies are screened as are described, for example, in Harlow & Lane, Antibodies, A Laboratory Manual, Cold Spring Harbor Press, New York (1988); Goding, Monoclonal antibodies, Principles and Practice (2d ed.) Academic Press, New York (1986). Monoclonal antibodies are tested for specific immunoreactivity with a variant gene product and lack of immunoreactivity to the corresponding prototypical gene product. These antibodies are useful in diagnostic assays for detection of the variant form, or as an active ingredient in a pharmaceutical composition.

V. Kits

[0057] The invention further provides kits comprising at least one sequence-specific probe as described above. Often, the kits contain one or more pairs of sequence-specific probes hybridizing to different forms of a polymorphism. In some kits, the sequence-specific probes are provided immobilized to a substrate. For example, the same substrate can comprise sequence-specific probes for detecting at least 10, 100 or all of the variations shown in Table 1. Optional additional components of the kit include, for example, restriction enzymes, reverse-transcriptase or polymerase, the substrate nucleoside triphosphates, means used to label (for example, an avidin-enzyme conjugate and enzyme substrate and chromogen if the label is biotin), and the appropriate buffers for reverse transcription, PCR, or hybridization reactions. Usually, the kit also contains instructions for carrying out the methods.

VI. Computer Databases

[0058] FIG. 1 illustrates an example of a computer system that can be used to store records relating to polymorphisms of the invention and perform algorithms comparing polymorphic profiles and to classify species. FIG. 2 shows a computer system 100 which includes a monitor 102, screen 104, cabinet 106, keyboard 108, and mouse 110. Mouse 110 may have one or more buttons such as mouse buttons 112. Cabinet 106 houses a CD-ROM drive 114, a system memory and a hard drive (see FIG. 2) which can be utilized to store and retrieve software programs incorporating code that implements the present invention, data for use with the present invention, and the like. Although a CD-ROM 116 is shown as an exemplary computer readable storage medium, other computer readable storage media including floppy disks, tape, flash memory, system memory, and hard drives may be utilized. Cabinet 106 also houses familiar computer components such as a central processor, system memory, hard disk, and the like.

[0059] FIG. 2 shows a system block diagram of computer system 100 that may be used to execute software embodiments of the present invention. As in FIG. 1, computer system 100 includes monitor 102 and keyboard 108. Computer system 100 further includes subsystems such as a central processor 102, system memory 120, I/O controller 122, display adapter 124, removable disk 126 (e.g., CD-ROM drive), fixed disk 128 (e.g., hard drive), network interface 130, and speaker 132. Other computer systems suitable for use with the present invention may include additional or fewer subsystems. For example, another computer system can include more than one processor 102 (i.e., a multi-processor system) or a cache memory.

[0060] Arrows such as 134 represent the system bus architecture of computer system 100. However, these arrows are illustrative of any interconnection scheme serving to link the subsystems. For example, a local bus can be utilized to connect the central processor to the system memory and display adapter. Computer system 100 shown in FIG. 1 is but an example of a computer system suitable for use with the present invention.

[0061] The computer stores records relating to the polymorphisms of the record. Some such records record a polymorphism by reference to the position of a polymorphic site and the identity of base(s) occupying that site in one or more species. Some databases include records for at least ten polymorphic sites in at least ten of the sequences shown in Table 1. Some databases include records for all of the polymorphic sites in at least one of the sequences shown in Table 1. Some databases includes records for at least 100, 1000, or 2000 polymorphic sites shown in Table 1. Some databases include records for all of the polymorphic sites shown in Table 1.

[0062] The foregoing invention has been described in some detail by way of illustration and example, for purposes of clarity and understanding. It will be obvious to one of skill in the art that changes and modifications may be practiced within the scope of the appended claims. Therefore, it is to be understood that the above description is intended to be illustrative and not restrictive. The scope of the invention should, therefore, be determined not with reference to the above description, but should instead be determined with reference to the following appended claims, along with the full scope of equivalents to which such claims are entitled.

[0063] All patents, patent applications and publications cited in this application are hereby incorporated by reference in their entirety for all purposes to the same extent as if each individual patent, patent application or publication were so individually denoted. TABLE-US-00001 TABLE 2 Affy# SAMPLE ID# Alt. ID SPECIES MY621 ATCC M. abscessus ATCC1 25291 ATCC-av M. avium M100 60300 MAC M. avium M101 60112 MAC M. avium M102 60268 MAC M. avium M103 60270 MAC M. avium M104 60272 MAC M. avium M105 60293 MAC M. avium M106 60313 MAC M. avium M107 60345 MAC M. avium M29 95-1764 M. avium M30 95-1766 M. avium M31 95-1768 M. avium M32 95-1770 M. avium M33 95-1775 M. avium M34 95-1776 M. avium M48 95-1765 M. avium M49 95-1769 M. avium M63 MAC #1 MAC M. avium M64 MAC #2 MAC M. avium M65 MAC #3 MAC M. avium M67 MAC #5 MAC M. avium M69 MAC #7 MAC M. avium M70 MAC #8 MAC M. avium M71 MAC #9 MAC M. avium M72 MAC #10 MAC M. avium M91 FM avium-intracell. M. avium FM(MAC) M92 60040 MAC M. avium M93 60042 MAC M. avium M94 60049 MAC M. avium M95 60051 MAC M. avium M96 60110 MAC M. avium M97 60116 MAC M. avium M98 60123 MAC M. avium M99 60176 MAC M. avium M78 92-773 M. bovis MY451 M. bovis ATCC2 35752 ATCC-chel(new) M. chelonae M10 95A9151 M. chelonae M11 95A0477 M. chelonae M115 60121 M. chelonae M116 52942 M. chelonae M117 43192 M. chelonae M118 53180 M. chelonae M119 53131 M. chelonae M12 95A4883 M. chelonae M120 52923 M. chelonae M121 52919 M. chelonae M13 95A2611 M. chelonae M14 95A0779 M. chelonae M15 95A8654 M. chelonae M16 95A8882 M. chelonae M17 95A8881 M. chelonae M50 95A11814 M. chelonae M51 95A1102 M. chelonae M75 #13 MAC#13 M. chelonae MY109 M. chelonae MY200 M. chelonae MY207 M. chelonae MY209 M. chelonae M122 60025 M. flavescens M123 60078 M. flavescens M124 60252 M. flavescens ATCC3 6841 ATTC-for M. fortuitum M53 60305 M. fortuitum M54 60344 M. fortuitum M55 60435 M. fortuitum M56 60447 M. fortuitum M74 #12 MAC#12 M. fortuitum M88 CH font. CH M. fortuitum MY221 M. fortuitum MY223 M. fortuitum MY225 M. fortuitum MY341 M. fortuitum MY715 M. fortuitum MY470 M. genevese ATCC4 14470 ATCC-go M. gordonae ATCC4-0 gord. M. gordonae M125 60068 M. gordonae M126 60182 M. gordonae M127 60214 M. gordonae M128 60283 M. gordonae M78 92-942 gord. LZ M. gordonae M79 93-692 gord. JD M. gordonae M80 94-94 gord. LG M. gordonae M81 93-1231 gord. LL M. gordonae M82 93-463 gord. RM M. gordonae M83 92-1219 gord. MB M. gordonae M84 91-1131 gord. OW M. gordonae M85 91-1478 gord. LB M. gordonae M86 92-642 gord. RB M. gordonae M87 93-1180 gord. WN M. gordonae M90 DB gord. DB M. gordonae MY103 M. gordonae MY475 M. gordonae MY476 M. gordonae My746 M. gordonae MY830 M. gordonae ATCC5 ATCC-int M. intracellulare ATCC5-0 intra M. intracellulare M18 95-1778 M. intracellulare M19 95-1780 M. intracellulare M20 94-1781 M. intracellulare M21 95-1782 M. intracellulare M22 95-1790 M. intracellulare M23 95-1794 M. intracellulare M24 95-1796 M. intracellulare M25 95-1777 M. intracellulare M26 95-1779 M. intracellulare M27 95-1760 M. intracellulare M28 95-1761 M. intracellulare ATCC6 12478 ATTC-kan M. kansasii ATCC6-0 kans. M. kansasii M1 95A5375 M. kansasii M2 95A10299 M. kansasii M3 96A0020 M. kansasii M4 95A3977 M. kansasii M5 95A4739 M. kansasii M52 95A5381 M. kansasii M57 60163 M. kansasii M58 60180 M. kansasii M59 60207 M. kansasii M59 95A2695 M. kansasii M60 60294 M. kansasii M61 60308 M. kansasii M62 60314 M. kansasii M7 95A2694 M. kansasii M73 #11 MAC#11 M. kansasii M8 94A9042 M. kansasii M9 95A1275 M. kansasii MY106 M. kansasii MY141 M. kansasii MY216 M. kansasii MY218 M. kansasii MY226 M. kansasii M108 60044 M. malmoense M109 60149 M. malmoense M110 60211 M. malmoense M111 60202 M. malmoense M112 60085 M. malmoense M113 60047 M. malmoense M114 60185 M. malmoense MY325 ATCC M. malmoense MY718 maimo M. malmoense MY214 M. marinum MY224 M. marinum MY244 M. marinum MY339 M. marinum MY343 M. marinum MY458 ATCC M. mucogenicum MY809 M. mucogenicum MY817 M. mucogenicum MY821 M. mucogenicum MY824 M. mucogenicum MY102 M. nonchromagenicum MY105 M. nonchromagenicum MY251 M. nonchromagenicum MY256 M. nonchromagenicum MY294 M. nonchromagenicum ATCC7 19981 ATCC-scr M. scrofulaceum ATCC7-0 scrof. M. scrofulaceum MY121 M. scrofulaceum MY249 M. scrofulaceum MY372 M. scrofulaceum MY387 M. scrofulaceum MY484 M. sinniae MY556 M. sinniae MY583 M. sinniae MY586 M. sinniae ATCC8 19420 ATCC-sme M. smegmatis M35 95A1072 M. smegmatis M36 95A8183 M. smegmatis M37 95A4990 M. smegmatis M77 92-144 smeg. JL M. smegmatis MY143 ATCC M. smegmatis MY104 M. szulgai MY198 M. szulgai MY357 M. szulgai MY358 M. szulgai MY480 M. szulgai TB74 C.17.96.5 M. tab M160 DR MY387 M. tb MY418 M. tb MY437 M. tb MY462 M. tb TB59 C.18.96.1 M. tb H37rv DR TB57 C.16.96.1 M. tb H37rv DR TB73 C.17.96.1 M. tb H37rv DR TB60 C.18.96.2 M. tb J35 DR TB65 C.22.96.9 M. tb M101 DR TB82 C.18.96.4 M. tb M104 DR TB89 C.16.96.3 M. tb M104 DR TB72 C.16.98.7 M. tb M104 DR TB66 C.22.96.10 M. tb M112 DR TB63 C.18.96.5 M. tb M140 DR TB64 C.18.96.6 M. tb M160 DR TB70 C.18.96.4 M. tb M160 DR TB61 C.18.96.3 M. tb M60 DR TB68 C.18.96.2 M. tb M60 DR TB71 C.18.96.5 M. tb M60 DR MY212 M. terrae MY354 M. terrae MY491 M. terrae MY497 M. terrae MY816 M. triplex ATCC9 27294 Mtb M. tuberculosis ATCC9-0 TB2020 M. tuberculosis N/A 93-1071 M. tuberculosis N/A 93-336 M. tuberculosis N/A 92-852 M. tuberculosis N/A 92-1005 M. tuberculosis N/A 92-243 M. tuberculosis N/A 92-304 M. tuberculosis N/A 92-199 M. tuberculosis N/A 92-197 M. tuberculosis N/A 92-484 M. tuberculosis N/A 94-577 M. tuberculosis TB1 936 M. tuberculosis TB10 1122 M. tuberculosis TB11 3407 M. tuberculosis TB12 978 M. tuberculosis TB13 3553 M. tuberculosis TB14 3468 M. tuberculosis TB15 2163 M. tuberculosis TB16 DW DW M. tuberculosis TB17 CB CB M. tuberculosis TB18 PB PB M. tuberculosis TB19 AA AA M. tuberculosis TB2 M0404A M. tuberculosis TB20 3492 M. tuberculosis TB21 1435 M. tuberculosis TB22 896 M. tuberculosis TB23 2268 M. tuberculosis TB24 3455 M. tuberculosis TB25 37 M. tuberculosis TB26 173 M. tuberculosis TB27 230 M. tuberculosis TB28 2519 M. tuberculosis TB29 T29233 M. tuberculosis TB3 1231 M. tuberculosis

TB30 SP SP M. tuberculosis TB31 3201 M. tuberculosis TB32 3219 M. tuberculosis TB33 80 M. tuberculosis TB34 3442 M. tuberculosis TB35 3502 M. tuberculosis TB36 3759 M. tuberculosis TB37 1295 M. tuberculosis TB38 337 M. tuberculosis TB39 394 M. tuberculosis TB4 914 M. tuberculosis TB40 499 M. tuberculosis TB41 535 M. tuberculosis TB42 607 M. tuberculosis TB43 707 M. tuberculosis TB44 692 M. tuberculosis TB45 2408 M. tuberculosis TB46 1069 M. tuberculosis TB47 M3282A M. tuberculosis TB48 1338 M. tuberculosis TB49 1368 M. tuberculosis TB5 1145 M. tuberculosis TB50 65 M. tuberculosis TB51 727 M. tuberculosis TB52 3455 M. tuberculosis TB53 3506 M. tuberculosis TB54 9500387 M. tuberculosis TB55 9600173 M. tuberculosis TB56 9503471 M. tuberculosis TB57 9600309 M. tuberculosis TB58 9600230 M. tuberculosis TB6 1417 M. tuberculosis TB7 SM2341 M. tuberculosis TB75 2098 M. tuberculosis TB76 173/1 M. tuberculosis TB77 1122/1 M. tuberculosis TB78 1417/1 M. tuberculosis TB8 1587 M. tuberculosis TB9 M7032A M. tuberculosis ATCC10 19250 ATTC-xen M. xenopi M129 60133 M. xenopi M130 60200 M. xenopi M131 60365 M. xenopi M132 60387 M. xenopi M38 95A5208 M. xenopi M39 95A5399 M. xenopi M40 95A3938 M. xenopi M41 95A8782 M. xenopi M42 95A0933 M. xenopi M43 95A4320 M. xenopi M44 95A478 M. xenopi M45 95A2997 M. xenopi M46 95A8383 M. xenopi M47 95A4319 M. xenopi M89 SG xen.SG M. xenopi MY219 M. xenopi MY250 M. xenopi MY252 M. xenopi MY254 M. xenopi MY255 M. xenopi MY107 MAC MY111 MAC MY112 MAC MY312 MAC M66 MAC #4 MAC #4 unique

[0064]

Sequence CWU 1

1

181 1 705 DNA Mycobacterium tuberculosis 1 cccaggacgt ggaggcgatc acaccgcaga cgttgatcaa catccggccg gtggtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccaatt catggaccag aacaacccgc 120 tgtcggggtt gacccacaag cgccgactgt cggcgctggg gcccggcggt ctgtcacgtg 180 agcgtgccgg gctggaggtc cgcgacgtgc acccgtcgca ctacggccgg atgtgcccga 240 tcgaaacccc tgaggggccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cgggttcatc gaaacgccgt accgcaaggt ggtcgacggc gtggttagcg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca cgtggtggca caggccaatt 420 cgccgatcga tgcggacggt cgcttcgtcg agccgcgcgt gctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtctgagg tggactacat ggacgtctcg ccccgccaga 540 tggtgtcggt ggccaccgcg atgattccct tcctggagca cgacgacgcc aaccgtgccc 600 tcatgggggc aaacatgcag cgccaggcgg tgccgctggt ccgtagcgag gccccgctgg 660 tgggcaccgg gatggagctg cgcgcggcga tcgacgcggc gacgt 705 2 626 DNA Mycobacterium abscessus 2 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggcctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc tcgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggctcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg gtttcatcga gacgccttac cggaaggtct 300 cggacggagt tgtcaccgac gacatccact acctgacggc cgacgaagag gaccgccacg 360 tggtggcgca ggccaactcg cccgtggacg ccaacggccg cttcaccgag gagaagatcc 420 tggttcgccg caagggcggc gaggtggagt tcgtgtcggc gaccgaggtc gactacatgg 480 atgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gtagcgaggc tccgctggtc ggtacc 626 3 705 DNA Mycobacterium avium modified_base (525)...(525) n = g,a,c or t 3 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cggcaagggc cggttcgccg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacntktcs ccgcgccara 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcak cgccaggcgg ttccgctggt gcgcagcgan gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 4 705 DNA Mycobacterium avium 4 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 5 705 DNA Mycobacterium avium 5 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 6 705 DNA Mycobacterium avium 6 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 7 705 DNA Mycobacterium avium 7 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 8 705 DNA Mycobacterium avium 8 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 9 705 DNA Mycobacterium avium 9 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 10 705 DNA Mycobacterium avium 10 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccr gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg cctggaggtc cgtgacgtgc acccgtcsca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg taygcgcggg 300 tsaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtsgtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgagg agkcccgggt gctggtccgc cgsaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 11 705 DNA Mycobacterium avium modified_base (42)...(42) n = g,a,c or t 11 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa cntccgtccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagt tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cggcttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgccg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tngacgcggc gacgt 705 12 705 DNA Mycobacterium avium 12 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 13 705 DNA Mycobacterium avium 13 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 14 705 DNA Mycobacterium avium 14 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 15 626 DNA Mycobacterium avium 15 tccgtccagt cgtggcggcg atcaaggagt tcttcggcac cagccagctg tcccagttca 60 tggaccagaa caacccgctg tcggggctca cccacaagcg ccgcctgtcg gcgctgggcc 120 cgggtggtct gtcccgggag cgggccgggc tggaggtccg cgacgtgcac ccgtcccact 180 acggccggat gtgcccgatc gagaccccgg agggtcccaa catcggtctg atcggctcgc 240 tgtcggtgta tgcgcgggtc aacccgttcg ggttcatcga gacgccgtac cgcaaggtgg 300 tcgacggcgt ggtcaccgac gagatccact acctgaccgc cgacgaggag gaccgccacg 360 tggtggcgca ggccaactcg ccgatcgacg acaagggccg gttcgcggag gcccgggtgc 420 tggtccgccg caaggcgggc gaggtcgagt acgtgccctc gtccgaggtg gactacatgg 480 acgtgtcgcc gcgccagatg gtgtcggtgg ccaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctg atgggcgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc gccgctggtg ggcacc 626 16 705 DNA Mycobacterium avium 16 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 17 705 DNA Mycobacterium avium 17 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgaa gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 18 687 DNA Mycobacterium avium 18 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 19 687 DNA Mycobacterium avium 19 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 20 687 DNA Mycobacterium avium 20 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 21 687 DNA Mycobacterium avium 21 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60

gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 22 687 DNA Mycobacterium avium 22 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 23 687 DNA Mycobacterium avium 23 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 24 705 DNA Mycobacterium avium 24 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc 120 tgtcggggct cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg 180 agcgggccgg gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 25 687 DNA Mycobacterium avium 25 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 26 687 DNA Mycobacterium avium 26 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 attcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggctc gctgtcggtg tacgcccggg tcaacccgtt 300 cggcttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg accagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcgg 420 cgcggacggc agcttcaccg aagaccgcgt gatggtccgc cgtaagggcg gcgaggtcga 480 gaacgtggcc ccgatcgacg tggattacat ggacgtctcg ccgcgccaga tggtgtcggt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgtagcgag gccccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 27 687 DNA Mycobacterium avium complex (MAC) 27 ggaggcgatc acaccgcaga ccctgatcaa catccgtcca gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtcggggct 120 cacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccggg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtccca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tatgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggc gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgacaagggc cggttcgcgg aggcccgggt gctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 28 705 DNA Mycobacterium bovis 28 cccaggacgt ggaggcgatc acaccgcaga cgttgatcaa catccggccg gtggtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccaatt catggaccag aacaacccgc 120 tgtcggggtt gacccacaag cgccgactgt cggcgctggg gcccggcggt ctgtcacgtg 180 agcgtgccgg gctggaggtc cgcgacgtgc acccgtcgca ctacggccgg atgtgcccga 240 tcgaaacccc tgaggggccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cgggttcatc gaaacgccgt accgcaaggt ggtcgacggc gtggttagcg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca cgtggtggca caggccaatt 420 cgccgatcga tgcggacggt cgcttcgtcg agccgcgcgt gctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtctgagg tggactacat ggacgtctcg ccccgccaga 540 tggtgtcggt ggccaccgcg atgattccct tcctggagca cgacgacgcc aaccgtgccc 600 tcatgggggc aaacatgcag cgccaggcgg tgccgctggt ccgtagcgag gccccgctgg 660 tgggcaccgg gatggagctg cgcgcggcga tcgacgcggc gacgt 705 29 687 DNA Mycobacterium bovis 29 ggaggcgatc acaccgcaga cgttgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgagccaatt catggaccag aacaacccgc tgtcggggtt 120 gacccacaag cgccgactgt cggcgctggg gcccggcggt ctgtcacgtg agcgtgccgg 180 gctggaggtc cgcgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgaaacccc 240 tgaggggccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gaaacgccgt accgcaaggt ggtcgacggc gtggttagcg acgagatcgt 360 gtacctgacc gccgacgagg aggaccgcca cgtggtggca caggccaatt cgccgatcga 420 tgcggacggt cgcttcgtcg agccgcgcgt gctggtccgc cgcaaggcgg gcgaggtgga 480 gtacgtgccc tcgtctgagg tggactacat ggacgtctcg ccccgccaga tggtgtcggt 540 ggccaccgcg atgattccct tcctggagca cgacgacgcc aaccgtgccc tcatgggggc 600 aaacatgcag cgccaggcgg tgccgctggt ccgtagcgag gccccgctgg tgggcaccgg 660 gatggagctg cgcgcggcga tcgacgc 687 30 652 DNA Mycobacterium chelonae 30 cgcagaccct gatcaacatc cgtcccgtcg tggcggcgat caaggagttc ttcggaacca 60 gccagctgtc gcagttcatg gaccagaaca acccgctgtc gggtctgacc cacaagcgtc 120 gtctgtcggc gctgggcccc ggtggtctga ctcgtgaccg cgccggcctt gaggtccgcg 180 acgtgcaccc ctcgcactac ggccgcatgt gcccgatcga gaccccggaa ggcccgaaca 240 tcggtctgat cggttcgctg tcggtgtacg cgcgggtcaa cccgttcggc ttcatcgaga 300 cgccgtaccg caaggtgtcc gagggtgtcg tcaccgacga gatccactac ctgaccgccg 360 acgaagagga ccgccacgtg gtggcgcagg ccaactcgcc tgtggatgcc gacggccgct 420 tcaccgagga caagatcctg gtccgccgta agggtggcga ggtcgagttc gtctcggcga 480 ccgaggtgga ctacatggac gtctcgccgc gccagatggt gtcggtcgcg accgccatga 540 tcccgttcct cgagcacgac gacgccaacc gtgccctcat gggtgccaac atgcagcgcc 600 aggcggttcc gctggtgcgc agcgaggccc cgctggtcgg taccggtatg ga 652 31 626 DNA Mycobacterium chelonae 31 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 32 647 DNA Mycobacterium chelonae 32 tgatcaacat ccgtcccgtc gtggcggcga tcaaggagtt cttcggaacc agccagctgt 60 cgcagttcat ggaccagaac aacccgcttt cgggtctgac ccacaagcgt cgtctgtcgg 120 ctctgggccc cggtggtctg acccgtgacc gcgctggcct tgaggtccgc gacgtgcacc 180 cctcgcacta cggccgcatg tgcccgatcg agaccccgga aggcccgaac atcggcctga 240 tcggttcgct ttcggtgtac gcgcgggtca acccgttcgg cttcatcgag acgccgtacc 300 gcaaggtgtc cgagggtgtc gtcaccgacg agatccacta cctgaccgcc gacgaagagg 360 accgccacgt cgtggcacag gccaactcgc ctgtggatgc cgacggccgc ttcaccgagg 420 acaagatcct ggtccgccgt aagggtggcg aggtcgagtt cgtctcggcg accgaggtgg 480 actacatgga cgtctcgccg cgccagatgg tgtcggtcgc gaccgccatg atcccgttcc 540 tcgagcacga cgacgccaac cgtgccctca tgggtgccaa catgcagcgc caggcggttc 600 cgctggtgcg cagcgaggcc ccgctggtcg gtaccggtat ggagctg 647 33 626 DNA Mycobacterium chelonae 33 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctttcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccaaatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 34 643 DNA Mycobacterium chelonae 34 tgatcaacat ccgtcccgtc gtggcggcga tcaaggagtt cttcggaacc agccagctgt 60 cgcagttcat ggaccagaac aacccgcttt cgggtctgac ccacaagcgt cgtctgtcgg 120 ctctgggccc cggtggtctg acccgtgacc gcgctggcct tgaggtccgc gacgtgcacc 180 cctcgcacta cggccgcatg tgcccgatcg agaccccgga aggcccgaac atcggcctga 240 tcggttcgct ttcggtgtac gcgcgggtca acccgttcgg cttcatcgag acgccgtacc 300 gcaaggtgtc cgagggtgtc gtcaccgacg agatccacta cctgaccgcc gacgaagagg 360 accgccacgt cgtggcacag gccaactcgc ctgtggatgc cgacggccgc ttcaccgagg 420 acaagatcct ggtccgccgt aagggtggcg aggtcgagtt cgtctcggcg accgaggtgg 480 actacatgga cgtctcgccg cgccagatgg tgtcggtcgc gaccgccatg atcccgttcc 540 tcgagcacga cgacgccaac cgtgccctca tgggtgccaa catgcagcgc caggcggttc 600 cgctggtgcg cagcgaggcc ccgctggtcg gtaccggtat gga 643 35 705 DNA Mycobacterium chelonae 35 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcgga accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg cccgggtggt ctgacccgtg 180 accgcgccgg ccttgaggtc cgtgacgtgc acccctcgca ctatggccgc atgtgcccga 240 tcgagacccc ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcgcg 300 ttaacccgtt cggcttcatc gagacgccgt accgcaaggt ggtcgagggt gtcgtcaccg 360 acgagatccg ctacctgact gccgacgaag aggaccgcca cgtggtggcg caggccaact 420 cgccgaccga tgagaagggc cgcttcaccg aggagcgcgt cctggtgcgc cgtaagggtg 480 gcgaggtcga gttcgtgccg tcgacgggcg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt cgcgaccgcg atgatcccgt tcctggagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgtagcgag gccccgctgg 660 tcggtaccgg tatggagctg cgcgcggcga tcgacgcggc gacgt 705 36 626 DNA Mycobacterium chelonae 36 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 37 626 DNA Mycobacterium chelonae 37 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 38 652 DNA Mycobacterium chelonae 38 gcagaccctg atcaacatcc gtcccgtcgt ggcggcgatc aaggagttct tcggaaccag 60 ccagctgtcg cagttcatgg accagaacaa cccgctgtcg ggtctgaccc acaagcgtcg 120 tctttcggcg ctgggccccg gtggtctgac ccgtgaccgc gccggccttg aggtccgcga 180 cgtgcacccc tcgcactacg gccgcatgtg cccgatcgag accccggaag gcccgaacat 240 cggcctgatc ggttcgctgt cggtgtacgc gcgggtcaac ccgttcggct tcatcgagac 300 gccgtaccgc aaggtgtccg agggtgtcgt caccgacgag atccactacc tgaccgccga 360 cgaagaggac cgccacgtcg tggcacaggc caactcgcct gtggatgccg acggccgctt 420 caccgaggac aagatcctgg tccgccgtaa gggtggcgag gtcgagttcg tctcggcgac 480 cgaggtggac tacatggacg tctcgccgcg ccaaatggtg tcggtcgcga ccgccatgat 540 cccgttcctc gagcacgacg acgccaaccg tgccctcatg ggtgccaaca tgcagcgcca 600 ggcggttccg ctggtgcgca gcgaggcccc gctggtcggt accggtatgg ag 652 39 626 DNA Mycobacterium chelonae 39 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gacatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggacg ccgacggccg tttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 40 626 DNA Mycobacterium chelonae 40 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctttcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggactg atcggttcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tggtggcgca ggccaactcg cctgtggatg cggacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 41 626 DNA Mycobacterium chelonae 41 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc

626 42 626 DNA Mycobacterium chelonae 42 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 43 626 DNA Mycobacterium chelonae 43 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 44 626 DNA Mycobacterium chelonae 44 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgtctgtcg gctctgggcc 120 ccggtggtct gacccgtgac cgcgctggcc ttgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtgt 300 ccgagggtgt cgtcaccgac gagatccact acctgaccgc cgacgaagag gaccgccacg 360 tcgtggcaca ggccaactcg cctgtggatg ccgacggccg cttcaccgag gacaagatcc 420 tggtccgccg taagggtggc gaggtcgagt tcgtctcggc gaccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 45 626 DNA Mycobacterium chelonae 45 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggcctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc tcgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggctcgc 240 tgtcggtgta cgcgcgcgtc aacccgttcg gtttcatcga gacgccttac cggaaggtct 300 cggacggagt tgtcaccgac gagatccact acctgacggc cgacgaagag gaccgccacg 360 tggtggcgca ggccaactcg cccgtggacg ccaacggccg cttcaccgag gagaagatcc 420 tggttcgccg caagggcggc gaggtggagt tcgtgtcggc gaccgaggtc gactacatgg 480 atgtttcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gtagcgaggc tccgctggtc ggtacc 626 46 626 DNA Mycobacterium chelonae 46 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggcctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc tcgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggctcgc 240 tgtcggtgta cgcgcgcgtc aacccgttcg gtttcatcga gacgccttac cggaaggtct 300 cggacggagt tgtcaccgac gagatccact acctgacggc cgacgaagag gaccgccacg 360 tggtggcgca ggccaactcg cccgtggacg ccaacggccg cttcaccgag gagaagatcc 420 tggttcgccg caagggcggc gaggtggagt tcgtgtcggc gaccgaggtc gactacatgg 480 atgtttcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gtagcgaggc tccgctggtc ggtacc 626 47 626 DNA Mycobacterium chelonae 47 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggcctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gacccgtgac cgcgccggcc tcgaggtccg cgacgtgcac ccctcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggctcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg gtttcatcga gacgccttac cggaaggtct 300 cggacggagt tgtcaccgac gacatccact acctgacggc cgacgaagag gaccgccacg 360 tggtggcgca ggccaactcg cccgtggacg ccaacggccg cttcaccgag gagaagatcc 420 tggttcgccg caagggcggc gaggtggagt tcgtgtcggc gaccgaggtc gactacatgg 480 atgtctcgcc gcgccagatg gtgtcggtcg cgaccgccat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctc atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gtagcgaggc tccgctggtc ggtacc 626 48 687 DNA Mycobacterium flavescens 48 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tctcgggcct 120 gacccacaag cgccgcctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctcgaggtc cgcgacgtgc acgcatcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt caaggacggt gttgtcaccg atgacatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 tgacaacggc cgcttcctgg aggagcgcgt cctggtccgc cgcaagggcg gcgaggtcga 480 gcagatctcg tcgagcgagg tggactacat ggacgtctcg ccgcgccaga tggtatcggt 540 cgcgacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gccccgctgg tcggcaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 49 687 DNA Mycobacterium flavescens 49 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tctcgggcct 120 gacccacaag cgccgcctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctcgaggtc cgcgacgtgc acgcgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt caaggacggt gttgtcaccg atgacatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 tgacaacggc cgcttcctgg aggagcgcgt cctggtccgc cgcaagggcg gcgaggtcga 480 gcagatctcg tcgagcgagg tggactacat ggacgtctcg ccgcgccaga tggtatcggt 540 cgcgacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gccccgctgg tcggcaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 50 687 DNA Mycobacterium flavescens 50 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc tgtcgggcct 120 gacccacaag cgccgcctgt cggcgctggg ccccggtggt ctgtcccgtg agcgtgccgg 180 cctcgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga tcgagacccc 240 ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagaccccgt accgcaaggt cgtcgacggc gtcgtcagcg accagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaatt cgccgctcga 420 cggtgacggt cgtttcgagg aggagcgcgt cctggtccgc cgtaagggcg gcgaggtcga 480 gttcgtctcg gcgagcgagg tcgactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 cgcgacggcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 gaacatgcag cgccaggcgg ttccgctggt ccgcagcgag gcgccgttgg tcggtaccgg 660 catggaactg cgcgcggcga tcgacgc 687 51 705 DNA Mycobacterium fortuitum 51 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg 300 tcaacccgtt cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cccggacggc cggttcaccg aggaccgcgt gatggttcgt cgtaagggcg 480 gcgaggtcga gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtccgt cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg 660 tcggtaccgg tatggagctg cgcgcggcga tcgacgcggc gacgt 705 52 705 DNA Mycobacterium fortuitum 52 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga attcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccg aacatcggtc tgatcggctc gctgtcggtg tacgcccggg 300 tcaacccgtt cggcttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcgg cgcggacggc agcttcaccg aagaccgcgt gatggtccgc cgtaagggcg 480 gcgaggtcga gaacgtggcc ccgatcgacg tggattacat ggacgtctcg ccgcgccaga 540 tggtgtcggt cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgtagcgag gccccgctgg 660 tcggtaccgg tatggagttg cgcgcggcga tcgacgcggc gacgt 705 53 626 DNA Mycobacterium fortuitum modified_base (340)...(340) n = g,a,c or t 53 tccgtcccgt cgtggcggcg atcaaggagt tcttcggtac cagccagctg tcgcagttca 60 tggaccagaa caacccgctt tcgggtctga cccacaagcg tcgcctgtcg gcgctgggcc 120 ccggcggtct gtcccgtgag cgtgccggcc ttgaggtccg cgacgtgcac gccagccact 180 acggccgcat gtgcccgatc gagacccctg agggtccgaa catcggtctg atcggctcgc 240 tgtcggtgta cgcccgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtcg 300 tcgacggtgt ggtcaccgac cagatcgact acctgaccgn cgacgaggag gacnntcacg 360 tcgtggcgca ggccaactcg ccgatcgacg ccgacggccg cttcaccgaa gaccgcgtca 420 tggtgcgtcg taagggcggc gaggtcgaga acgtggcccc gtccgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtctgtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcca acatgcagcg ccaggcggtt ccactggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 54 705 DNA Mycobacterium fortuitum 54 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgtccga 240 tcgagacccc tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg 300 tcaacccgtt cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg 360 atcagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cccggacggc cggttcaccg aggaccgcgt gatggttcgt cgtaagggcg 480 gcgaggtcga gaatgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtccgt cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg 660 tcggtaccgg tatggagctg cgcgcggcga tcgacgcggc gacgt 705 55 705 DNA Mycobacterium fortuitum 55 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgcctgt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgtgccgg ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccg aacatcggtc tgatcggttc gctgtcggtg tacgcccggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt cgtcgacggt gtggtctccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgcggacggc agcttcaccg aggatcgcgt gatggtccgg cgtaagggtg 480 gcgaggtcga gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtctgt cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg 660 tcggtaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 56 626 DNA Mycobacterium fortuitum 56 tccgtcccgt cgtggcggcg atcaaggagt tcttcggaac gtcgcagctg tcgcagttca 60 tggatcagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggcggtct gtcccgtgag cgcgccggcc ttgaggtccg cgacgtccac tcgtcgcact 180 acggccgcat gtgtccgatc gagacccctg agggtccgaa catcggtctg atcggttcgc 240 tttcggtgta cgcgcgggtc aacccgttcg gtttcatcga gaccccgtac cgcaaggtcg 300 tcgacggtgt ggtcaccgat cakatckact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgatcgacc cggacggccg gttcaccgag gaccgcgtga 420 tggttcgtcg taagggcggc gaggtcgaga atgtggcccc gtccgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtccgtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 57 705 DNA Mycobacterium smegmatis 57 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgcccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccg aacatcggtc tgatcggctc gctgtcggtg tacgcccggg 300 tcaacccgtt cggcttcatc gagacgcctt accgcaaggt tgtcgacggt gtggtcagcg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga caccgacggt cgcttcaccg aggaccgcgt gatggtccgc cgtaagggtg 480 gcgaggtcga gaacgtggcc ccgtccgacg tcgactacat ggacgtctca ccgcgccaga 540 tggtgtctgt cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcag ttccgctggt acgcagcgag gccccgctgg 660 tcggtaccgg tatggagctg cgcgcggcga tcgacgcggc gacgt 705 58 687 DNA Mycobacterium chelonae 58 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgcctgt cggcgctggg ccccggcggt ctgtcccgtg agcgtgccgg 180 ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggttc gctgtcggtg tacgcccggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt cgtcgacggt gtggtctccg accagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcggacggc agcttcaccg aggatcgcgt gatggtccgc cgtaagggtg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga tggtgtctgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg tcggtaccgg 660 catggagttg cgcgcggcga tcgacgc 687 59 687 DNA Mycobacterium fortuitum 59 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg aycagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cccggacggc cggttcaccg aggaccgcgt gatggttcgc cgtaagggcg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga tggtgtccgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 60 687 DNA Mycobacterium fortuitum 60 ggaggcgatc acaccgcaga ccctgatcaa catccgcccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtgc actccagcca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggctc gctgtcggtg tacgcccggg tcaacccgtt 300 cggcttcatc gagacgcctt accgcaaggt tgtcgacggt gtggtcagcg accagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 caccgacggt cgcttcaccg aggaccgcgt gatggtccgc cgtaagggtg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctca ccgcgccaga tggtgtctgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcag ttccgctggt acgcagcgag gccccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 61 687 DNA Mycobacterium fortuitum 61 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg atcagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cccggacggc cggttcaccg aggaccgcgt gatggttcgt cgtaagggcg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga tggtgtccgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 62 687 DNA Mycobacterium fortuitum 62 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg tcaacccgtt 300

cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg atcagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cccggacggc cggttcaccg aggaccgcgt gatggttcgt cgtaagggcg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga tggtgtccgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 63 687 DNA Mycobacterium fortuitum 63 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcgga acgtcgcagc tgtcgcagtt catggatcag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 ccttgaggtc cgcgacgtcc actcgtcgca ctacggccgc atgtgtccga tcgagacccc 240 tgagggtccg aacatcggtc tgatcggttc gctttcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtggtcaccg atcagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cccggacggc cggttcaccg aggaccgcgt gatggttcgt cgtaagggcg gcgaggtcga 480 gaacgtggcc ccgtccgacg tcgactacat ggacgtctcg ccgcgccaga tggtgtccgt 540 cgcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gccccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 64 626 DNA Mycobacterium fortuitum 64 tccgtccggt cgttgccgcg atcaaggagt tcttcggaac cagccagctg tcgcagttca 60 tggaccagaa caacccgctc tccggtctca cccacaagcg ccgcctctcg gcgctggggc 120 cgggcggtct gtcccgtgag cgcgccggtc tggaagttcg tgacgtgcac ccgtcgcact 180 acggccggat gtgcccgatc gagacgccgg aagggccgaa catcggtctg atcggttcac 240 tgtcggtgta cgcccgggtc aacccgttcg ggttcatcga gacgccctac cgcaaggtgg 300 tcgacggggt cgtttccgac gagatccact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgatcgacg cgcagggccg cttcgtcgag ccgcgcgtgc 420 tggtccgccg gaaggcgggc gaggtcgagt acgtgccctc gtcagaggtg gactacatgg 480 acgtgtcgcc gcgccagatg gtgtcggtgg ccaccgcgat gattccgttc ctcgagcacg 540 atgacgccaa ccgcgccttg atgggtgcca acatgcagcg scaggcggtc ccgctggtgc 600 gcagcgaggc accgctggtc ggtacc 626 65 705 DNA Mycobacterium gordonae 65 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 66 705 DNA Mycobacterium gordonae modified_base (90)...(90) n = g,a,c or t 66 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagn tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggt gctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctaacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 67 705 DNA Mycobacterium gordonae 67 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tttcgggcct cacccacaag cgtcgtctgt cggcgctggg gcccggcggt ctgtcccgtg 180 agcgggccgg cctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagactcc ggaaggcccc aacatcggcc tgatcggctc actgtcggtg tacgcgcggg 300 tgaacccgtt cggcttcatc gagacgccgt atagacgagt ggtgagcgga gttgtcacgg 360 atgagatcca ctacctcacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgagaacggc cggtttgtcg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt ccgcagcgar gcgccgctgg 660 tgggtaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 68 705 DNA Mycobacterium gordonae 68 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtggccg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgcctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgccgg cctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cgggttcatc gagacgccgt accgcaaggt ggtggaaggt gtcgtctccg 360 acgaaatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggt cggttcgtcg agccacgcgt tctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgcct tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgar gcaccgctgg 660 tgggcaccgg tatggaattg cgcgcggcga tcgacgcggc gacgt 705 69 705 DNA Mycobacterium gordonae 69 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tttcgggcct cacccacaag cgtcgtctgt cggcgctggg gcccggcggt ctgtcccgtg 180 agcgggccgg cctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagactcc ggaaggcccc aacatcggcc tgatcggctc actgtcggtg tacgcgcggg 300 tgaacccgtt cggcttcatc gagacgccgt atagacgagt ggtgagcgga gttgtcacgg 360 atgagatcca ctacctcacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgagaacggc cggttcgtcg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tcgactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggtaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 70 705 DNA Mycobacterium gordonae 70 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgctg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tttcgggcct cacccacaag cgtcgtctgt cggcgctggg gcccggcggt ctgtcccgtg 180 agcgggccgg cctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagactcc ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cggcttcatc gagacgccgt accgcgaggt ggtcgacggt gtggtgacgg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgagaacggc cgcttcgtcg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt ccgcagcgag gcgccgctgg 660 tgggtaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 71 705 DNA Mycobacterium gordonae 71 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 72 705 DNA Mycobacterium gordonae 72 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 73 705 DNA Mycobacterium gordonae modified_base (690)...(690) n = g,a,c or t 73 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tttcgggcct cacccacaag cgtcgtctgt cggcgctggg gcccggcggt ctgtcccgtg 180 agcgggccgg cctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagactcc ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cggcttcatc gagacgccgt accgcgaggt ggtcgacggt gtggtgacgg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgagaacggc cgcttcgtcg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt ccgcagcgag gcgccgctgg 660 tgggtaccgg catggagttg cgcgcggcgn tcgacgcggc gacgt 705 74 705 DNA Mycobacterium gordonae 74 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 75 705 DNA Mycobacterium gordonae 75 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctct cggcgctggg gccgggtggt ctgtcccgtg 180 agcgcgccgg tctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggaaggtccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgccgt accgggaggt tgtggacggg gtcgtcacag 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagaacggc cggtttgtcg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgtagcgar gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 76 705 DNA Mycobacterium gordonae 76 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 77 705 DNA Mycobacterium gordonae 77 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gactcacaag cgtcgtctgt cggcgctggg gcctggcggt ctgtcacgtg 180 agcgcgccgg cctggaagtc cgtgacgtcc acccgtcgca ctacggccgg atgtgcccga 240 tcgagacccc ggaaggcccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtcgacggt gtggtctccg 360 atgagatcca ctacctgacc gccgacgaag gagaccccca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgccg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtcccg tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg tatggagttg cgcgcggcga tcgacgcggc gacgt 705 78 705 DNA Mycobacterium gordonae modified_base (688)...(688) n = g,a,c or t 78 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg 180 agcgtgcggg tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacgcc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg 360 acgagatcca ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggcaccgg catggagttg cgcgcggnga tcgacgcggc nacnn 705 79 705 DNA Mycobacterium gordonae 79 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaaccccc 120 tgtcgggtct cacccacaag cggcgtctgt cggcgctcgg gccgggtggt ctgtcgcgtg 180 agcgtgcggg tctggaagtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggaaggtccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgcctt atagacgcgt cgtcagcgga gttgtcacgg 360 atgagatcca ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgctggc cggtgcttct cactttgctg agccgcgcgt tctggtccgc cgcaaggcgg 480 gcgaggtgga gtacgttccg tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 80 687 DNA Mycobacterium gordonae 80 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg agcgtgcggg 180 tctggaagta cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga tcgagacgcc 240 ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg acgagatcca 360 ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg gcgaggtgga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 81 687 DNA Mycobacterium gordonae 81 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg tccgggtggt ctgtcccgtg agcgcgccgg 180 tctggaggtc cgtgacgtcc acccgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggaaggtccg aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgccgt accgggaggt tgtggacggg gtcgttacag acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgagagcggc cggtttgtcg agccgcgcgt tctggtccgc cgcaaggcgg gcgaggtgga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggtaccgg 660 catggagctg cgcgcggcga tcgacgc 687 82 687 DNA Mycobacterium gordonae 82 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtctgt cggcgctggg gccgggtggt ctgtcccgtg agcgtgcggg 180 tctggaagta cgtgacgtgc acccgtcgca

ctacggccgc atgtgcccga tcgagacgcc 240 ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgcctt atcggaaggt ggtggatgga gtcgtttctg acgagatcca 360 ctacctcacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgagagcggc cggtttgctg agccgcgcgt tctggtccgc cgcaaggcgg gcgaggtgga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgaaca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 83 687 DNA Mycobacterium gordonae modified_base (47)...(47) n = g,a,c or t 83 ggaggcgatc acaccgcaga ctctgatcaa catccggccc gtcgtcnccg cgatcaagga 60 gttnttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtccggttt 120 gacgcacaag aggcgtctgt ccgcgctggg gccgggtggt ctgtcccgtg agcgggccgg 180 cctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggaaggtccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccct atcggaaggt ggtggacggg gtcgtctcgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgagaacggc cgcttcgtcg agccgcgtgt gctggtccgc cggaaggcgg gcgaggtgga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgtagcgag gcgccgttgg tgggcaccgg 660 gatggagttg cgcgcggcga tcgacgc 687 84 705 DNA Mycobacterium gordonae 84 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 85 705 DNA Mycobacterium intracellulare 85 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaagcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 86 705 DNA Mycobacterium intracellulare 86 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 87 705 DNA Mycobacterium intracellulare 87 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 88 705 DNA Mycobacterium intracellulare 88 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 89 705 DNA Mycobacterium intracellulare 89 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccctt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 90 705 DNA Mycobacterium intracellulare 90 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 91 705 DNA Mycobacterium intracellulare 91 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccctt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 92 705 DNA Mycobacterium intracellulare 92 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 93 705 DNA Mycobacterium intracellulare 93 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 94 705 DNA Mycobacterium intracellulare 94 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 95 705 DNA Mycobacterium intracellulare 95 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtca ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 96 705 DNA Mycobacterium intracellulare 96 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc 120 tgtccggtct gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tgaacccgtt cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt 705 97 687 DNA Mycobacterium avium 97 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc tgtccggtct 120 gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tgaacccctt 300 cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccactggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 98 687 DNA Mycobacterium avium complex (MAC) 98 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc tgtccggtct 120 gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tgaacccctt 300 cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 99 687 DNA Mycobacterium avium complex (MAC) 99 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc tgtccggtct 120 gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tgaacccgtt 300 cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccaagggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 100 687 DNA Mycobacterium avium complex (MAC) 100 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tgagccagtt catggaccag aacaacccgc tgtccggtct 120 gacccacaag cgccgcctct cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctggaggtc cgtgacgtcc acccctcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tgaacccgtt 300 cgggttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccaagggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgatcccgt tcctcgagca cgatgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 101 705 DNA Mycobacterium kansasii 101 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 102 705 DNA Mycobacterium kansasii 102 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa

catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtt cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 103 705 DNA Mycobacterium kansasii 103 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 104 705 DNA Mycobacterium kansasii 104 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 105 705 DNA Mycobacterium kansasii 105 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 106 705 DNA Mycobacterium kansasii 106 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgt 120 tgtcgggcct gacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccgtg 180 agcgtgccgg cctggaagtg cgtgacgtgc acccttcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggatc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgccgt accggaaggt gatcgacggg ctggtcaccg 360 atgagatcca ctacctgacg gccgacgaag aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgacggc cgctttgtcg agccgcgcgt tctggtgcgc cgcaaggcgg 480 gcgaggtcga atacgtcgcc tcctccgagg tggactacat ggacgtctcg ccacgccaaa 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgggcac 600 tgatgggcgc caacatgcag cgtcaggcgg ttccgctggt acgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 107 705 DNA Mycobacterium kansasii 107 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 108 705 DNA Mycobacterium kansasii 108 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 109 626 DNA Mycobacterium kansasii 109 tccgcccggt ggtcgccgcc atcaaggagt tcttcggcac cagccagctc tcccagttca 60 tggaccagaa caacccgctg tcgggcctca cccacaagcg ccggctttcg gcgctggggc 120 cgggcggtct gtcccgggag cgtgccgggc tggaagtgcg tgacgtgcac ccgtcgcact 180 acggccgcat gtgcccgatc gagaccccgg agggtcccaa catcggcctg atcggctcgc 240 tgtcggtgta cgcacgggtc aacccgttcg gcttcatcga gacgccgtac cgcaaggtga 300 tcgacggtct cgttactgat gagatccact acttgacggc cgacgaggag gaccgccacg 360 tcgtggcaca ggccaactcg ccgatcgacg ctgagggccg gttcgtcgag ccgcgcgtgc 420 tggtgcgccg caaggccggc gaggtcgagt acgtggcctc gtcggaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtgg ccacggccat gattccgttc ctcgagcacg 540 acgacgccaa ccgggctctg atgggtgcca acatgcagcg ccaggcggtt ccgctggtgc 600 gcagcgaggc gccgctggtg ggcacc 626 110 705 DNA Mycobacterium kansasii 110 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 111 705 DNA Mycobacterium kansasii 111 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 112 705 DNA Mycobacterium kansasii 112 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 113 705 DNA Mycobacterium kansasii 113 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgccggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 114 705 DNA Mycobacterium kansasii 114 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 115 705 DNA Mycobacterium kansasii 115 cccaggacgt ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg 60 ccatcaagga gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc 120 tgtcgggcct cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg 180 agcgtgccgg gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg 360 atgagatcca ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact 420 cgccgatcga cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg 480 gcgaggtcga gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 116 687 DNA Mycobacterium kansasii 116 ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg ccatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggcct 120 cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg agcgtgccgg 180 gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg atgagatcca 360 ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact cgccgatcga 420 cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg gcgaggtcga 480 gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 117 687 DNA Mycobacterium kansasii 117 ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg ccatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggcct 120 cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg agcgtgccgg 180 gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg atgagatcca 360 ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact cgccgatcga 420 cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg gcgaggtcga 480 gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 118 687 DNA Mycobacterium kansasii 118 ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg ccatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggcct 120 cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg agcgtgccgg 180 gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg atgagatcca 360 ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact cgccgatcga 420 cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg gcgaggtcga 480 gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 119 687 DNA Mycobacterium kansasii 119 ggaggcgatc acaccgcaga cactgatcaa catccgcccg gtggtcgccg ccatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggcct 120 cacccacaag cgccggcttt cggcgctggg gccgggcggt ctgtcccggg agcgtgccgg 180 gctggaagtg cgtgacgtgc acccgtcgca ctacggccgc atgtgcccga tcgagacccc 240 ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcacggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt gatcgacggt ctcgttactg atgagatcca 360 ctacttgacg gccgacgagg aggaccgcca cgtcgtggca caggccaact cgccgatcga 420 cgctgagggc cggttcgtcg agccgcgcgt gctggtgcgc cgcaaggccg gcgaggtcga 480 gtacgtggcc tcgtcggagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgggctc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 120 699 DNA Mycobacterium malmoense 120 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 121 699 DNA Mycobacterium malmoense 121 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 122 699 DNA Mycobacterium malmoense 122 ggaggcgatc acaccgcaga cgctgatcaa catccggccg

gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 123 699 DNA Mycobacterium malmoense 123 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 124 699 DNA Mycobacterium malmoense 124 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 125 699 DNA Mycobacterium malmoense 125 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 126 699 DNA Mycobacterium malmoense 126 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcggggct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcgcgtg agcgtgccgg 180 cttggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg tcaatccgtt 300 cgggttcatc gagacgcctt atcggaaggt tgtggacggt gtcgttactg acgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccag 420 acccaacgag gccggtgccg aggttttcga agaggggcgt gtcctggttc gccgcaaggc 480 gggcgaggtg gagtacgtgc ccagctccga ggtggactac atggacgtct cgccgcggca 540 gatggtgtcc gtggccaccg ccatgattcc gttcctcgag cacgacgacg ccaaccgtgc 600 cctgatgggc gccaacatgc agcgccaggc ggttccgctg gtgcgcagcg aggcgccgct 660 ggtgggcacc ggcatggagc tgcgcgcggc gatcgacgc 699 127 687 DNA Mycobacterium marinum 127 ggaggcgatc acaccgcaga cgttgatcaa catccgtccg gtcgttgccg cgatcaagga 60 gttcttcgga accagccagc tgtcgcagtt catggaccag aacaacccgc tctccggtct 120 cacccacaag cgccgcctct cggcgctggg gccgggcggt ctgtcccgtg agcgcgccgg 180 tctggaagtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacgcc 240 ggaagggccg aacatcggtc tgatcggttc actgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccct accgcaaggt ggtcgacggg gtcgtttccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgcagggc cgcttcgtcg agccgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtcagagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgatgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcaccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 128 687 DNA Mycobacterium marinum 128 ggaggcgatc acaccgcaga cgttgatcaa catccgtccg gtcgttgccg cgatcaagga 60 gttcttcgga accagccagc tgtcgcagtt catggaccag aacaacccgc tctccggtct 120 cacccacaag cgccgcctct cggcgctggg gccgggcggt ctgtcccgtg agcgcgccgg 180 tctggaagtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacgcc 240 ggaagggccg aacatcggtc tgatcggttc actgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccct accgcaaggt ggtcgacggg gtcgtttccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgcagggc cgcttcgtcg agccgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtcagagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgatgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcaccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 129 687 DNA Mycobacterium marinum 129 ggaggcgatc acaccgcaga cgctgatcaa catccgtccg gtcgttgccg cgatcaagga 60 gttcttcgga accagccagc tgtcgcagtt catggaccag aacaacccgc tctccggtct 120 cacccacaag cgccgcctct cggcgctggg gccgggcggt ctgtcccgtg agcgcgccgg 180 tctggaagtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacgcc 240 ggaagggccg aacatcggtc tgatcggttc actgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccct accgcaaggt ggtcgacggg gtcgtttccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgcagggc cgcttcgtcg agccgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtcagagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgatgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcaccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 130 687 DNA Mycobacterium marinum 130 ggaggcgatc acaccgcaga cgctgatcaa catccgtccg gtcgttgccg cgatcaagga 60 gttcttcgga accagccagc tgtcgcagtt catggaccag aacaacccgc tctccggtct 120 cacccacaag cgccgcctct cggcgctggg gccgggcggt ctgtcccgtg agcgcgccgg 180 tctggaagtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacgcc 240 ggaagggccg aacatcggtc tgatcggttc actgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccct accgcaaggt ggtcgacggg gtcgtttccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgcagggc cgcttcgtcg agccgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtcagagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgatgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcaccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 131 687 DNA Mycobacterium marinum 131 ggaggcgatc acaccgcaga cgctgatcaa catcsgtccg gtcgttgccg cgatcaagga 60 gttcttcgga accagccagc tgtcgcagtt catggaccag aacaacccgc tctccggtct 120 cacccacaag cgccgcctct cggcgctggg gccgggcggt ctgtcccgtg agcgcgccgg 180 tctggaagtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacgcc 240 ggaagggccg aacatcggtc tgatcggttc actgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccct accgcaaggt ggtcgacggg gtcgtttccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgcagggc cgcttcgtcg agccgcgcgt gctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccc tcgtcagagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgatgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcaccgctgg tcggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 132 626 DNA Mycobacterium mucogenicum 132 tccgtcccgt cgtggcggcg atcaaggagt tcttcggtac gtcgcagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gtcccgtgag cgcgccggcc tcgaggtccg cgacgtccac tcgtcgcact 180 acggccgcat gtgcccgatc gagacccctg aaggcccgaa catcggtctg atcggctcgc 240 tgtcggtgta cgcgcgggtg aacccgttcg gcttcatcga gaccccgtac cgcaaggtcg 300 tcgacggcat cgtcaccgat cagatcgact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgctggacg cgaacggcca cttcaccgag gagaagatcc 420 tcgtccgtcg taagggcggc gaggtcgagt tcgtctcggc gaacgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgcgat gatcccgttc ctggagcacg 540 acgacgccaa ccgcgccctc atgggtgcga acatgcagcg tcaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 133 626 DNA Mycobacterium mucogenicum 133 tccgtcccgt cgtggcggcg atcaaggagt tcttcggcac gtcgcagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gtcccgtgag cgcgccggcc tcgaggtccg cgacgtccac tcgtcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggtctg atcggctcgc 240 tgtcggtgta cgcacgggtc aacccgttcg gcttcatcga gaccccgtac cgcaaggtcg 300 tcgacggcat cgtcaccgat cagatcgact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgctggacg cgaacggcca cttcaccgag gagaagatcc 420 tcgtccgtcg taagggcggc gaggtcgagt tcgtctcggc gaacgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcga acatgcagcg tcaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 134 626 DNA Mycobacterium mucogenicum 134 tccgtcccgt cgtggcggcg atcaaggagt tcttcggcac gtcgcagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gtcccgtgag cgcgccggcc tcgaggtccg cgacgtccac tcgtcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggtctg atcggctcgc 240 tgtcggtgta cgcacgggtc aacccgttcg gcttcatcga gaccccgtac cgcaaggtcg 300 tcgacggcat cgtcaccgat cagatcgact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgctggacg cgaacggcca cttcaccgag gagaagatcc 420 tcgtccgtcg taagggcggc gaggtcgagt tcgtctcggc gaacgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcga acatgcagcg tcaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 135 626 DNA Mycobacterium mucogenicum modified_base (8)...(8) n = g,a,c or t 135 tccgtccngt cgtggcggcg atcaaggagt tcttcggcac gtcgcagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gtcccgtgag cgcgccggcc tcgaggtccg cgacgtccac tcgtcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggtctg atcggctcrc 240 tgtcggtgta cgcacgggtc aacccgttcg gcttcatcga gaccccgtac cgcaaggtcg 300 tcgacggcat cgtcaccgat cagatcgact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgctggacg cgaacggcca cttcaccgag gagaagatcc 420 tcgtccgtcg taagggcggc gaggtcgagt tcgtctcggc gaacgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcga acatgcagcg tcaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 136 626 DNA Mycobacterium mucogenicum 136 tccgtcccgt cgtggcggcg atcaaggagt tcttcggcac gtsgcagctg tcgcagttca 60 tggaccagaa caacccgctg tcgggtctga cccacaagcg tcgtctgtcg gcgctgggcc 120 ccggtggtct gtcccgtgag cgcgccggcc tcgaggtycg cgacgtccac tcgtcgcact 180 acggccgcat gtgcccgatc gagaccccgg aaggcccgaa catcggtctg atcggctcrc 240 tgtcggtgta cgcacgggtc aacccgttcg gcttcatcga gaccccgtac cgcaaggtcg 300 tcgacggcat cgtcaccgat cagatcgact acctgaccgc cgacgaggag gaccgccacg 360 tcgtggcgca ggccaactcg ccgctggacg cgaacggcca cttcaccgag gagaagatcc 420 tcgtccgtcg taagggcggc gaggtcgagt tcgtctcggc gaacgacgtc gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgaccgcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgcgccctg atgggtgcga acatgcagcg tcaggcggtt ccgctggtgc 600 gcagcgaggc cccgctggtc ggtacc 626 137 687 DNA Mycobacterium nonchromagenicum 137 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgt tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg accgggcggt ctgtcgcgtg agcgggccgg 180 cctggaagtt cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagacgccct accgcaaggt cgtggacggg gtcgtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggaggacggg cacttcaccg aggaccgggt tctggttcgt cgtaagggtg gtgaggtcga 480 gtacgtgtcg tccgccgagg tcgactacat ggacgtctca ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagtgag gcgccgctgg tgggtaccgg 660 catggagctg cgcgcggcga tcgacgc 687 138 687 DNA Mycobacterium nonchromagenicum 138 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgt tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg accgggcggt ctgtcgcgtg agcgggccgg 180 cctggaagtt cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagacgccct accgcaaggt cgtggacggg gtcgtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggaggacggc cacttcaccg aggaccgggt tctggttcgc cgtaagggtg gcgaggtcga 480 gtacgtctcg tccgccgagg tcgactacat ggacgtctca ccgcgccaga tggtgtcggt 540 ggccacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagtgag gcgccgctgg tgggtaccgg 660 catggagctg cgcgcggcga tcgacgc 687 139 687 DNA Mycobacterium nonchromagenicum 139 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgt tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg accgggcggt ctgtcgcgtg agcgggccgg 180 cctggaagtt cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagacgccct accgcaaggt cgtggacggg gtcgtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgttgtggcg caggccaact cgccgctgga 420 ggaggacggc cacttcaccg aggaccgggt tctggttcgt cgtaagggtg gtgaggtcga 480 gtacgtctcg tccgccgagg tcgactacat ggacgtctca ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagtgag gcgccgctgg tgggtaccgg 660 catggagctg cgcgcggcga tcgacgc 687 140 687 DNA Mycobacterium nonchromagenicum 140 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgt tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg accgggcggt ctgtcgcgtg agcgggccgg 180 cctggaagtt cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagacgccct accgcaaggt cgtggacggg gtcgtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgttgtggcg caggccaact cgccgctgga 420 ggaggacggc cacttcaccg aggaccgggt tctggttcgt cgtaagggtg gtgaggtcga 480 gtacgtctcg tccgccgagg tcgactacat ggacgtctca ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagtgag gcgccgctgg tgggtactgg 660 catggagctg cgcgcggcga tcgacgc 687 141 687 DNA Mycobacterium nonchromagenicum 141 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgt tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg accgggcggt ctgtcgcgtg agcgggccgg 180 cctggaagtt cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggtttcatc gagacgccct accgcaaggt cgtggacggg gtcgtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggaggacggc cacttcaccg aggaccgggt tctggttcgt cgtaagggtg gtgaggtcga 480 gtacgtgtcg tccgccgagg tcgactacat ggacgtctca ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagtgag gcgccgctgg tgggtaccgg 660 catggagctg cgcgcggcga tcgacgc 687 142 687 DNA Mycobacterium terrae 142 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccggctgt cggcgctggg gcccggtggt ctgtcccgtg agcgcgccgg

180 cctggaagtt cgtgacgtgc acccgagcca ctacggccgg atgtgtccga tcgagacccc 240 ggaaggcccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tgaacccgtt 300 cggcttcatc gagacgccct accgcaaggt ggtcgacggt gtcgtcagcg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggacgacggc cggttcgccg aggaacgagt tctggtgcgc cgcaagggcg gcgaggtcga 480 gtacgtgtcg tcggccgagg ttgactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgattccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgtcaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 143 705 DNA Mycobacterium scrofulaceum 143 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggcct gacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccgcg 180 agcgggccgg gctggaggtc cgggacgtgc acccgtcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgtca cgtcgtggcg caggccaact 420 cgccgatcga cgcgagcggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 144 705 DNA Mycobacterium scrofulaceum 144 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tgtcgggcct gacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccgcg 180 agcgggccgg gctggaggtc cgggacgtgc acccgtcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacacgtt cggcttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcaccg 360 acgagatcca ctacctgacc gccgacgagg aggaccgtca cgtcgtggcg caggccaact 420 cgccgatcga cgcgagcggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg 480 gcgaggtcga gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagttg cgcgcggcga tcgacgcggc gacgt 705 145 687 DNA Mycobacterium scrofulaceum 145 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtggtggccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccgcctgt cggcgctggg cccgggcggt ctgtcccgtg agcgggccgg 180 cctcgaggtg cgcgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggcc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt cgtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgcgagcggc cggttcgagg agtcgcgcgt gctggtccgc cggaaggccg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg tcccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 146 687 DNA Mycobacterium scrofulaceum 146 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtggccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggcct 120 gacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccgcg agcgggccgg 180 gctggaggtc cgggacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgtca cgtcgtggcg caggccaact cgccgatcga 420 cgcgagcggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 147 687 DNA Mycobacterium scrofulaceum 147 ggaggcgatc acaccgcaga cgctgatcaa catccggccg gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcccagtt catggaccag aacaacccgc tgtcgggtct 120 gacgcacaag cgccgcctgt cggcgctggg ccccggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgcgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggaggggccg aacatcggtc tgatcggctc gctgtcggtg tacgcccggg tcaacccgtt 300 cggcttcatc gagaccccgt accgcaaggt ggtcgacggt gtggtcaccg acgagattca 360 ctacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgcgaacggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgtcaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 148 687 DNA Mycobacterium scrofulaceum 148 ggaggcgatc acaccgcaga ccctgatcaa catccggccg gtcgtggccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggcct 120 gacccacaag cgccgcctgt cggcgctggg cccgggtggt ctgtcccgcg agcgggccgg 180 gctggaggtc cgggacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccc aacatcggtc tgatcggctc gctgtcggtg tacgcgcggg tcaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcaccg acgagatcca 360 ctacctgacc gccgacgagg aggaccgtca cgtcgtggcg caggccaact cgccgatcga 420 cgcgagcggc cggttcgagg agtcgcgcgt cctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagttg cgcgcggcga tcgacgc 687 149 687 DNA Mycobacterium scrofulaceum 149 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccgcctct cggcgctggg acccggtggt ctgtcccgtg agcgtgcggg 180 cctcgaggtc cgtgacgtac acccgtcgca ctacggccgg atgtgtccga tcgaaacccc 240 ggaggggccg aacatcggtc tgatcgggtc gctgtcggtg tacgcccggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt tgtcgacggt gtggtcaccg acgagatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacggg cgcttcgaag agtcgcgtgt gctggttcgc cgcaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 cgccacggcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggcaccgg 660 gatggagttg cgcgcggcga tcgacgc 687 150 687 DNA Mycobacterium scrofulaceum 150 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 tacccacaag cgccgcctgt cggcgctggg gccgggcggt ctgtcccgtg agcgggcggg 180 cctcgaggtc cgcgatgtgc acccgtcgca ctacggccgg atgtgtccga tcgagacccc 240 cgagggtccg aacatcggtc tgatcgggtc gctatcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt tgtcgacggt gtggtcaccg acgagatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 ccccgacggc cgcttcgaag agtcgcgcgt gctggttcgc cgtaaggcgg gcgaggtcga 480 atacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggcgaccgcg atgatcccgt tcctcgaaca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt acgcagcgag gccccgctgg tcggcaccgg 660 gatggagctg cgcgcggcga tcgacgc 687 151 687 DNA Mycobacterium scrofulaceum 151 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 tacccacaag cgccgcctgt cggcgctggg gccgggcggt ctgtcccgtg agcgggcggg 180 cctcgaggtc cgcgatgtgc acccgtcgca ctacggccgg atgtgtccga tcgagacccc 240 cgagggtccg aacatcggtc tgatcgggtc gctatcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt tgtcgacggt gtggtcaccg acgagatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 ccccgacggc cgcttcgaag agtcgcgcgt gctggttcgc cgtaaggcgg gcgaggtcga 480 atacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggcgaccgcg atgatcccgt tcctcgaaca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt acgcagcgag gccccgctgg tcggcaccgg 660 gatggagctg cgcgcggcga tcgacgc 687 152 687 DNA Mycobacterium scrofulaceum 152 ggaggcgatc acaccgcaga ccctgatcaa catccgtccg gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 cacccacaag cgccgcctgt cggcgctggg gccgggcggt ctgtcccgtg agcgggcggg 180 cctcgaggtc cgagacgtgc acccgtcgca ctacggccgg atgtgtccga tcgagacccc 240 cgagggtccg aacatcggtc tgatcgggtc gctgtcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt tgtcgacggt gtggttaccg acgagatcga 360 gtacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact cgccgatcga 420 cgccgacggc cgcttcgaag agtcgcgcgt actggttcgc cgtaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggcgaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt acgcagcgag gccccgctgg tcggcaccgg 660 gatggagctg cgcgcggcga tcgacgc 687 153 705 DNA Mycobacterium smegmatis 153 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtcttt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgctgg cctcgaggtc cgcgacgtgc accccagcca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccc aacatcggtc tgatcggttc gctgtcggtg tacgcccgcg 300 tgaacccgtt cggcttcatc gagacgccgt accgcaaggt cgagaacggt gtggtcaccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgaccga cgagaacggc cgcttcaccg aggaccgcgt catggtccgc aagaagggcg 480 gcgaggtcga gttcgtctcc gccgaccagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt cgctacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggtaccgg tatggaactg cgcgcggcga tcgacgcggc gacgt 705 154 626 DNA Mycobacterium smegmatis modified_base (9)...(9) n = g,a,c or t 154 tccgtccgnt cgtggcggcg atcaaggagt tcttcggaac gtcgcagctg tcgcagttca 60 tggaccagaa caacccgctg tccggtctga cccacaagcg ccgcctgtcg gcgctgggcc 120 cgggtggtct gtcccgtgag cgcgccggcc tggaggtccg cgacgtgcac tccagccact 180 acggccggat gtgcccgatc gagaccccgg aaggcccgaa catcggcctg atcggttcgc 240 tgtcggtgta cgcgcgggtc aacccgttcg ggttcatcga gaccccgtac cgcaaggtga 300 tcgacggcca ggtcagcgat cagatcgact acctcaccgc cgacgaggag gaccgccaca 360 tcgtggcgca ggccaactcg ccgctcgacg acgagggccg gttcaccgag gacaagatcc 420 tcgtccgccg taagggcggc gaggtcgagt tcgtcgcggc caccgaggtg gactacatgg 480 acgtctcgcc gcgccagatg gtgtcggtcg cgacggcgat gatcccgttc ctcgagcacg 540 acgacgccaa ccgtgccctg atgggtgcca acatgcagcg ccaggcggtt ccgctggtcc 600 gcagcgaggc cccgctggtc ggcacc 626 155 705 DNA Mycobacterium smegmatis 155 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgcccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtctgt cggcgctggg cccgggcggt ctgtcccgtg 180 agcgcgccgg cctggaggtc cgcgacgtgc actccagcca ctacggccgg atgtgcccga 240 tcgagacccc ggaaggcccg aacatcggcc tgatcggttc gctgtcggtg tacgcgcggg 300 tgaacccgtt cggtttcatc gagaccccgt accgcaaggt cgtcgacggt gtcatcaccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgacaacggc cggttcaccg aggaccgcgt gctggtgcgc cgcaagggtg 480 gcgaggtcga gttcgtctcc gccaccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt cgcgacggcg atgatcccgt tcctcgagca cgacgacgcc aaccgtgcct 600 tgatgggtgc caacatgcag cgccaggccg ttccgctggt gcgcagcgag gccccgctgg 660 tcggcaccgg tatggagctg cgcgcggcga tcgacgcggc gacgt 705 156 705 DNA Mycobacterium smegmatis 156 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggtct gacccacaag cgtcgtcttt cggcgctggg ccccggcggt ctgtcccgtg 180 agcgcgccgg cctcgaggtc cgcgacgtgc accccagcca ctacggccgc atgtgcccga 240 tcgagacccc tgagggtccc aacatcggtc tgatcggttc gctgtcggtg tacgcccgcg 300 tgaacccgtt cggcttcatc gagacgcctt accgcaaggt cgagaacggt gtggtcaccg 360 accagatcga ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgaccga cgagaacggc cgcttcaccg aggaccgcgt catggtccgc aagaagggcg 480 gcgaggtcga gttcgtctcc gccgaccagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt cgctacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggtaccgg tatggaactg cgcgcggcga tcgacgcggc gacgt 705 157 705 DNA Mycobacterium gordonae modified_base (688)...(688) n = g,a,c or t 157 cccaggacgt ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc 120 tctccggtct gacgcacaag cggcgtctgt ccgctttggg gccgggcggt ctgtcccgtg 180 agcgggccgg gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga 240 tcgagacccc ggagggtccg aatatcggtc tgatcgggtc gctgtcggtg tacgcacggg 300 tcaacccgtt cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg 360 atgagatcca ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact 420 cgccgatcga cgccgacgga cggttcgtcg agggacgcgt cctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc 600 tgatgggtgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg 660 tgggtaccgg tatggagttg cgcgcggnga tcgacgcggn nacnn 705 158 687 DNA Mycobacterium smegmatis 158 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggcgg cgatcaagga 60 gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgtcgtcttt cggcgctggg ccccggcggt ctgtcccgtg agcgcgccgg 180 cctcgaggtc cgcgacgtgc accccagcca ctacggccgc atgtgcccga tcgagacccc 240 tgagggtccc aacatcggtc tgatcggttc gctgtcggtg tacgcccgcg tgaacccgtt 300 cggcttcatc gagacgccgt accgcaaggt cgagaacggt gtggtcaccg accagatcga 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgaccga 420 cgagaacggc cgcttcaccg aggaccgcgt catggtccgc aagaagggcg gcgaggtcga 480 gttcgtctcc gccgaccagg tggactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 cgccacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggaactg cgcgcggcga tcgacgc 687 159 687 DNA Mycobacterium szulgai 159 ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctccggtct 120 gacgcacaag cggcgtctgt ccgctttggg gccgggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aatatcggtc tgatcgggtc gctgtcggtg tacgcacggg tcaacccgtt 300 cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacgga cggttcgtcg agggacgtgt cctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 160 687 DNA Mycobacterium szulgai 160 ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctccggtct 120 gacgcacaag cggcgtctgt ccgctttggg gccgggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aatattggtc tgatcgggtc gctgtcggtg tacgcacggg tcaacccgtt 300 cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacgga cggttcgtcg agggacgtgt cctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 161 687 DNA Mycobacterium szulgai 161 ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctccggtct 120 gacgcacaag cggcgtctgt ccgctctggg gccgggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aatatcggtc tgatcgggtc gctgtcggtg tacgcacggg tcaacccgtt 300 cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacgga cggttcgtcg agggacgcgt cctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 162 687 DNA Mycobacterium szulgai 162 ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg cgatcaagga

60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctccggtct 120 gacgcacaag cggcgtctgt ccgctctggg gccgggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aatatcggtc tgatcgggtc gctgtcggtg tacgcacggg tcaacccgtt 300 cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacgga cggttcgtcg agggacgcgt cctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 163 687 DNA Mycobacterium szulgai 163 ggaggcgatc acaccgcaga ccctgatcaa catccggccc gtcgtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tctccggtct 120 gacgcacaag cggcgtctgt ccgctttggg gccgggcggt ctgtcccgtg agcgggccgg 180 gctggaggtc cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 ggagggtccg aatatcggtc tgatcgggtc gctgtcggtg tacgcacggg tcaacccgtt 300 cgggttcatc gagacgccgt atagacgcgt cgtcagcgga gttgtcacgg atgagatcca 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacgga cggttcgtcg agggacgtgt cctggtccgc cgcaaggcgg gcgaggtcga 480 gtacgtgccc tcctccgagg tggactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccaccgcg atgattccgt tcctcgagca cgacgacgcc aaccgcgccc tgatgggtgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcaccgctgg tgggtaccgg 660 tatggagttg cgcgcggcga tcgacgc 687 164 687 DNA Mycobacterium terrae 164 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgattaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcccgtg aacgggccgg 180 gcttgaggtc cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggagggtccg aacatcggtc tgatcggctc gctggcgact tacgcgcggg tcaacccgtt 300 cgggttcatc gaaaccccgt accgcaaggt caacgacggt gtggtcagcg atgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggacgacaat cgcttcaccg aggaccgggt tctggtgcgc cgcaagggcg gcgaggtcga 480 gtacgtgtcg tcggccgagg tcgactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgatcccgt tcctggagca cgacgacgcc aaccgggccc tgatgggtgc 600 caacatgcag cgtcaggcgg ttcccctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 165 687 DNA Mycobacterium terrae 165 ggaggcgatc acaccgcaga ccttgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcccgtg agcgggccgg 180 gcttgaggtc cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggagggtccg aacatcggtc tgatcggctc gctggcgact tacgcgcggg tcaacccgtt 300 cgggttcatc gaaaccccgt accgcaaggt caacgacggt gtggtcagcg atgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggacgacaat cgcttcaccg aggaccgggt tctggtgcgc cgcaagggcg gcgaggtcga 480 gtacgtgtcg tcggccgagg tcgactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgatcccgt tcctggagca cgacgacgcc aaccgggccc tgatgggtgc 600 caacatgcag cgtcaggcgg ttcccctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 166 687 DNA Mycobacterium terrae 166 ggaggcgatc acaccgcaga ccctgatcaa catccgcccg gtggtcgccg cgatcaagga 60 gttcttcggc accagccagc tctcgcagtt catggaccag aacaacccgc tgtcgggtct 120 gacccacaag cgccggctgt cggcgctggg cccgggtggt ctgtcccgtg agcgggccgg 180 gcttgaggtc cgtgacgtgc acccgtccca ctacggccgg atgtgtccga tcgagacccc 240 ggagggtccg aacatcggtc tgatcggctc gctggcgacc tacgcgcggg tcaacccgtt 300 cgggttcatc gaaaccccgt accgcaaggt caacgacggt gtggtcagcg atgagatcgt 360 ctacctgacc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgctgga 420 ggacgacagt cgcttcgccg aggaccgagt tctggtgcgc cgcaagggcg gtgaggtcga 480 gtacgtgtcg tcggccgagg tcgactacat ggacgtctcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgatcccgt tcctggagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgtcaggcgg ttcccctggt gcgcagcgag gcgccgctgg tgggcaccgg 660 catggagctg cgcgcggcga tcgacgc 687 167 687 DNA Mycobacterium triplex modified_base (139)...(139) n = g,a,c or t 167 ggaggcgatc acaccgcaga ccctgatcaa catccgtccc gtcgtggccg cgatcaagga 60 gttcttcggc accagccagc tgtcccagtt catggaccag aacaacccgc tgtccgggct 120 cacccacaag cgccgcctnt cggcgctggg gccgggcggt ctgtcgcgtg agcgcgcggg 180 cctcgaggtt cgtgacgtgc acccgtcgca ctacggccgg atgtgcccga tcgagacccc 240 cgagggtccg aacatcggtc tgatcggttc gctgtcggtg tacgcgcggg tcaacccgtt 300 cgggttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcaccg amcaratcga 360 ctasctgrcc gccgacgagg aggaccgcca cgtcgtggcg caggccaact cgccgatcga 420 cgccgacggc cggttcgagg agtcgcgtgt cctggtccgc cggaaggcgg gcgaggtcga 480 gtacgtgccg tcgtccgagg tcgactacat ggacgtgtcg ccgcgccaga tggtgtcggt 540 ggccacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc tgatgggcgc 600 caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg tcggtaccgg 660 tatggagctg cgcgcggcga tcgacgc 687 168 705 DNA Mycobacterium xenopi 168 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 169 705 DNA Mycobacterium xenopi 169 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 170 705 DNA Mycobacterium xenopi 170 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtakcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 171 705 DNA Mycobacterium xenopi 171 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 172 705 DNA Mycobacterium xenopi 172 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 173 705 DNA Mycobacterium xenopi 173 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 174 705 DNA Mycobacterium xenopi 174 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 175 705 DNA Mycobacterium xenopi 175 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 176 705 DNA Mycobacterium xenopi 176 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 177 705 DNA Mycobacterium xenopi 177 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 178 705 DNA Mycobacterium xenopi 178 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 179 705 DNA Mycobacterium xenopi 179 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 180 705 DNA Mycobacterium xenopi 180 cccaggacgt ggaggcgatc acaccgcaga ccttgatcaa catccgcccc gtggtggccg 60 cgatcaagga gttcttcggc accagccagc tctcgcagtt catggatcag aacaacccgc 120 tgtcggggct cacccacaag cggcggctct cggcgcttgg tccgggcggt ctgtcgcgcg 180 agcgggccgg gctggaggtc cgtgacgtgc actcgagcca ctacggccgg atgtgcccga 240 tcgaaacccc ggagggcccg aacatcggtt tgatcggctc gctgtcggtg tacgcgcggg 300 tcaacccgta cgggttcatt gagacgcctt accgcaaggt ggtcaacggc gtggtcaccg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca tgtggtggcg caggccaact 420 cgccgatcga cgaggatggc cgcttcaccg agccgcgggt gctggtgcgc cgcaagggtg 480 gggaggtcga gtacgtgtcc tcctccgagg tggactacat ggacgtctcg ccgcgccaga 540 tggtgtcggt ggccaccgcg atgatcccgt tcctcgagca cgacgacgcc aaccgcgcgt 600 tgatgggcgc gaacatgcag cgccaggccg ttccgttggt gcgtagcgag gcaccgctgg 660 tgggcaccgg gatggaattg cgcgcggcga tcgacgcggc gacgt 705 181 705 DNA Mycobacterium sp. unique MAC#4 181 cccaggacgt ggaggcgatc acaccgcaga cgctgatcaa catccgtccg gtcgtcgccg 60 cgatcaagga gttcttcggc accagccagc tgtcgcagtt catggaccag aacaacccgc 120 tgtcgggcct gacccacaag cgccgcctgt cggcgctggg cccgggcggt ctgtcccgtg 180 agcgcgccgg cctcgaggtc cgcgacgtgc acccgtcgca ctacggccgc atgtgcccga 240 tcgagacccc ggagggtccg aacatcggtc tgatcggctc gctgtcggtg tacgcgaggg 300 tcaacccgtt cggcttcatc gagacgccgt accgcaaggt ggtcgacggt gtggtcagcg 360 acgagatcgt gtacctgacc gccgacgagg aggaccgcca cgtggtggcg caggccaact 420 cgccgatcga cgccgacggc cggttcgtcg agggccgcgt cctggtccgc cgcaaggcgg 480 gcgaggtcga gtacgtgccc tcgtccgagg tggactacat ggacgtgtcg ccgcgccaga 540 tggtgtcggt ggccacggcc atgatcccgt tcctcgagca cgacgacgcc aaccgtgccc 600 tgatgggcgc caacatgcag cgccaggcgg ttccgctggt gcgcagcgag gcgccgctgg 660 tgggcaccgg catggagctg cgcgcggcga tcgacgcggc gacgt

705

Claims

1-19. (canceled)

20. A polynucleotide probe or primer that hybridizes under stringent hybridization conditions to a mycobacterial rpoB sequence selected from the group consisting of SEQ ID NOS: 2, 3, 4, 5, 6 and 9 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO: 1 or its complement, wherein said stringent hybridization conditions comprise 5.times.SSPE and a temperature of 25-30.degree. C.

21. The polynucleotide of claim 20 that is a probe.

22. The polynucleotide of claim 21, wherein a central position of the probe aligns with one or more bases of a sequence selected from the group consisting of SEQ ID NOS: 2, 3, 4, 5, 6 and 9 which differ from the corresponding one or more bases in SEQ ID NO: 1 when the sequences are maximally aligned.

23. The sequence-specific polynucleotide of claim 20 that is a primer.

24. The polynucleotide of claim 23, wherein the 3' end of the primer aligns with one or more bases of a sequence selected from the group consisting of SEQ ID NOS: 2, 3, 4, 5, 6 and 9 which differ from the corresponding one or more bases in SEQ ID NO: 1 when the sequences are maximally aligned.

25. The polynucleotide of claim 20 that hybridizes under stringent hybridization conditions to at least 100 contiguous bases of a mycobacterial rpoB sequence selected from the group consisting of SEQ ID NOS: 2, 3, 4, 5, 6 and 9 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO:1 or its complement, wherein said stringent hybridization conditions comprise 5.times.SSPE and a temperature of 25-30.degree. C.

26. A polynucleotide probe or primer that hybridizes under stringent hybridization conditions to at least 100 contiguous bases of a mycobacterial rpoB sequence selected from the group consisting of SEQ ID NOS: 8 and 10 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO:1 or its complement, wherein said stringent hybridization conditions comprise 5.times.SSPE and a temperature of 25-30.degree. C.

27. The polynucleotide of claim 26, wherein the polynucleotide is a probe, and wherein a central position of the probe aligns with one or more bases of a sequence selected from the group consisting of SEQ ID NOS: 8 and 10 which differ from the corresponding one or more bases in SEQ ID NO: 1 when the sequences are maximally aligned.

28. The polynucleotide of claim 26, wherein the polynucleotide is a primer, and wherein the 3' end of the primer aligns with one or more bases of a sequence selected from the group consisting of SEQ ID NOS: 8 and 10 which differ from the corresponding one or more bases in SEQ ID NO: 1 when the sequences are maximally aligned.