U.S. patent application number 11/344555 was filed with the patent office on 2006-06-15 for synergistic compositions that treat or inhibit pathological conditions associated with inflammatory response.
Invention is credited to John G. Babish, Jeffrey S. Bland, Gary K. Darland, Terrence Howell, Robert Lerman, DeAnn J. Liska, Daniel O. Lukaczer, Matthew L. Tripp.
Application Number | 20060127513 11/344555 |
Document ID | / |
Family ID | 32180687 |
Filed Date | 2006-06-15 |
United States Patent
Application |
20060127513 |
Kind Code |
A1 |
Tripp; Matthew L. ; et
al. |
June 15, 2006 |
Synergistic compositions that treat or inhibit pathological
conditions associated with inflammatory response
Abstract
A natural formulation of compounds that would to modulate
inflammation is disclosed. The formulation would also inhibit
expression of COX-2, inhibit synthesis of prostaglandins
selectively in target cells, and inhibit inflammatory response
selectively in target cells. The compositions containing at least
one fraction isolated or derived from hops. Other embodiments
relate to combinations of components, including at least one
fraction isolated or derived from hops, tryptanthrin and conjugates
thereof, rosemary, an extract or compound derived from rosemary, a
triterpene species, or a diterpene lactone or derivatives or
conjugates thereof.
Inventors: |
Tripp; Matthew L.; (Gig
Harbor, WA) ; Babish; John G.; (Brooktondale, NY)
; Bland; Jeffrey S.; (Fox Island, WA) ; Darland;
Gary K.; (Gig Harbor, WA) ; Lerman; Robert;
(Gig Harbor, WA) ; Lukaczer; Daniel O.; (Gig
Harbor, WA) ; Liska; DeAnn J.; (Tacoma, WA) ;
Howell; Terrence; (Lansing, NY) |
Correspondence
Address: |
McDermott Will & Emery LLP
28 State Street
Boston
MA
02109
US
|
Family ID: |
32180687 |
Appl. No.: |
11/344555 |
Filed: |
January 30, 2006 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10464410 |
Jun 18, 2003 |
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11344555 |
Jan 30, 2006 |
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10400293 |
Mar 26, 2003 |
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10464410 |
Jun 18, 2003 |
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10401283 |
Mar 26, 2003 |
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10464410 |
Jun 18, 2003 |
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60450237 |
Feb 25, 2003 |
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60420383 |
Oct 21, 2002 |
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Current U.S.
Class: |
424/778 ;
514/690 |
Current CPC
Class: |
A61K 36/53 20130101;
A61P 7/10 20180101; A61P 37/08 20180101; A61K 36/185 20130101; A61P
7/06 20180101; A61K 36/53 20130101; A61P 19/04 20180101; A61P 27/02
20180101; A61P 25/06 20180101; A61P 1/02 20180101; A61P 37/00
20180101; A61P 25/00 20180101; A61P 11/16 20180101; A61P 3/10
20180101; A61K 31/12 20130101; A61P 17/02 20180101; A61P 17/00
20180101; A61P 25/28 20180101; A61P 1/04 20180101; A61P 35/00
20180101; A61K 31/12 20130101; A61P 19/02 20180101; A61P 11/08
20180101; A61P 21/04 20180101; A61P 43/00 20180101; A61P 9/10
20180101; A61P 11/06 20180101; A61P 29/00 20180101; A61K 36/185
20130101; A61P 15/00 20180101; A61K 31/122 20130101; A61P 17/04
20180101; A61P 11/02 20180101; A61P 19/06 20180101; A61P 17/06
20180101; A61P 37/02 20180101; A61P 11/00 20180101; A61K 2300/00
20130101; A61K 2300/00 20130101; A61K 2300/00 20130101 |
Class at
Publication: |
424/778 ;
514/690 |
International
Class: |
A61K 36/185 20060101
A61K036/185; A61K 31/12 20060101 A61K031/12 |
Claims
1-86. (canceled)
87. A method of modulating the amount of cyclooxygenase-2 (COX-2)
activity in target cells without substantially modulating COX-2
activity in non-target cells, the method comprising contacting the
cells with a composition comprising a fraction isolated or derived
from hops and a second component selected from the group consisting
of rosemary, an extract derived from rosemary, a compound derived
from rosemary, a triterpene species, a diterpene lactone species,
and tryptanthrin.
88. The method of claim 87, wherein the non-target cells are also
contacted with said fraction isolated or derived from hops.
89. The method of claim 87, wherein the contacting step is in
vivo.
90. The method of claim 86, wherein the COX-2 activity is modulated
by inhibition of COX-2 gene.
91. A method of treating or inhibiting a pathological condition in
a mammal involving inhibiting inducibility or activity of
cyclooxygenase-2 (COX-2), the method comprising administering to
the mammal a composition comprising a fraction isolated or derived
from hops and a second component selected from the group consisting
of rosemary, an extract derived from rosemary, a compound derived
from rosemary, a triterpene species, a diterpene lactone, and
tryptanthrin.
92. The method of claim 91, wherein the fraction isolated or
derived from hops is selected from the group consisting of alpha
acids, isoalpha acids, reduced isoalpha acids; tetra-hydroisoalpha
acids, hexa-hydroisoalpha acids, beta acids, and spent hops.
93. The method of claim 91, wherein the fraction isolated or
derived from hops comprises a compound of a supragenus having the
formula: ##STR13## wherein R' is selected from the group consisting
of carbonyl, hydroxyl, OR, and OCOR, wherein R is alkyl; wherein
R'' is selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3; and
wherein R, T, X, and Z are independently selected from the group
consisting of H, F, Cl, Br, I, and .pi. orbital, with the proviso
that if one of R, T, X, or Z is a .pi. orbital, then the adjacent
R, T, X, or Z is also a .pi. orbital, thereby forming a double
bond.
94. The method of claim 91, wherein the fraction isolated or
derived from hops comprises a compound of Genus A having the
formula: ##STR14## wherein R' is selected from the group consisting
of carbonyl, hydroxyl, OR, and OCOR, wherein R is alkyl; and
wherein R'' is selected from the group consisting of
CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3.
95. The method of claim 91, wherein the fraction isolated or
derived from hops comprises a compound of Genus B having the
formula ##STR15## wherein R' is selected from the group consisting
of carbonyl, hydroxyl, OR, and OCOR, wherein R is alkyl; and
wherein R'' is selected from the group consisting of
CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3.
96. The method of claim 91, wherein the fraction isolated or
derived from hops comprises a compound selected from the group
consisting of humulone, cohumulone, adhumulone, isohumulone,
isocohumulone, isoadhumulone, dihydro-isohumulone,
dihydro-isocohumulone, dihydro-adhumulone, tetrahydro-isohumulone,
tetrahydro-isocohumulone, tetrahydro-adhumulone,
hexahydro-isohumulone, hexahydro-isocohumulone, and
hexahydro-adhumulone.
97. The method of claim 91, wherein the second component is an
extract derived from rosemary.
98. The method of claim 91, wherein the second component is a
triterpene species.
99. The method of claim 91, wherein the composition further
comprises a third component different from the second component,
the third component is selected from the group consisting of
rosemary, an extract derived from rosemary, a compound derived from
rosemary, a triterpene species, a diterpene lactone, and
tryptanthrin.
100. The method of claim 99, wherein the second and third
components are an extract derived from rosemary and tryptanthrin,
respectively.
101. The method of claim 91, wherein the second component is a
compound derived from rosemary that is selected from the group
consisting of 1,8-cineole, 19-alpha-hydroxyursolic acid,
2-.beta.-hydroxyoleanolic acid, 3-O-acetyloleanolic acid,
3-O-acetylursolic acid, 6-methoxy-luteolin-7-glucoside,
6-methoxyluteolin, 6-methoxyluteolin-7-glucoside,
methoxyluteolin-7-methylether, 7-ethoxy-rosmanol,
7-methoxy-rosmanol, alpha-amyrin, alpha-humulene,
alpha-hydroxyhydrocaffeic acid, alpha-pinene, alpha-terpinene,
alpha-terpinenyl acetate, alpha-terpineol, alpha-thujone, apigenin,
apigenin-7-glucoside, curcumene, benzyl-alcohol, .beta.-amyrenone,
.beta.-amyrin, .beta.-elemene, .beta.-pinene, betulin, betulinic
acid, borneol, bornyl-acetate, caffeic acid, camphene, camphor,
camosic acid, carnosol, carvacrol, carvone, caryophyllene,
caryophyllene-oxide, chlorogenic acid, diosmetin, gamma-terpinene,
hesperidin, isoborneol, limonene, luteolin,
luteolin-3'-O-(3''-O-acetyl)-.beta.-D-glucuronide,
luteolin-3'-O-(4''-O-acetyl)-.beta.-D-glucuronide,
luteolin-3'-O-P-D-glucuronide, luteolin-7-glucoside,
methyl-eugenol, myrcene, neo-chlorogenic acid, nepetin, octanoic
acid, oleanolic acid, p-cymene, piperitenone, rosmanol, rosmaric
acid, rosmaricine, rosmaridiphenol, rosemarinic acid, rosmarinol,
rosmariquinone, sabinene, sabinyl acetate, salicylates, salicylic
acid-2-.beta.-D-glucoside, squalene, terpinen-4-ol, terpinolene,
thymol, trans-anethole, trans-carveol, ursolic acid, verbenone, and
zingiberene.
102. The method of claim 101, wherein the second component is a
triterpene species or a diterpene lactone species that is
conjugated to a member selected from the group consisting of mono-
or di-saccharides, amino acids, sulfates, succinate, acetate, and
glutathione.
103. The method of claim 91, wherein the second component is a
triterpene species that is selected from the group consisting of
18-a-glycyrrhetinic acid, 18-.beta.-glycyrrhetinic acid,
2-a-3-a-dihydrooxyurs-12-3n-28-onic acid, 3-a-hydroxyursolic acid,
3-oxo-ursolic acid, betulin, betulinic acid, celastrol, eburicoic
acid, friedelin, glycyrrhizin, gypsogenin, oleanolic acid,
oleanolic acid-3-acetate, pachymic acid, pinicolic acid,
sophoradiol, soyasapogenol A, soyasapogenol B, tripterin,
triptophenolide, tumulosic acid, ursolic acid, ursolic
acid-3-acetate, uvaol, and .beta.-sitosterol.
104. The method of claim 91, wherein the second component is
tryptanthrin that is conjugated to a member selected from the group
consisting of mono- or di-saccharides, amino acids, sulfates,
succinate, acetate, and glutathione.
105. The method of claim 91, wherein a ratio of the first component
to the second component is in the range of about 100:1 to about
1:100.
106. The method of claim 105, wherein the ratio of the first
component to the second component is in the range of about 50:1 to
about 1:50.
107. The method of claim 91, wherein the pathological condition is
selected from the group consisting of inflammation,
inflammation-associated disorders, arthritis, asthma, bronchitis,
menstrual cramps, tendonitis, bursitis, skin-related conditions,
gastrointestinal conditions, cancer, ophthalmic diseases, pulmonary
inflammation, nervous system disorders, allergic rhinitis,
respiratory distress syndrome, endotoxin shock syndrome,
atherosclerosis, and central nervous damage.
108. The method of claim 91, wherein the composition further
comprises a pharmaceutically acceptable carrier.
109. The method of claim 91, wherein the composition is
administered orally, topically, parenterally, or rectally.
110-115. (canceled)
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This patent application is a continuation-in-part of U.S.
application Ser. No. 10/400,293, filed Mar. 26, 2003, and a
continuation-in-part of U.S. application Ser. No. 10/401,283, filed
Mar. 26, 2003, both of which claim the benefit under 35 U.S.C.
.sctn. 119(e) to provisional application No. 60/450,237, filed on
Feb. 25, 2003, and provisional application No. 60/420,383, filed on
Oct. 21, 2002. The contents of each of these earlier applications
are hereby incorporated by reference as if recited herein in their
entirety.
BACKGROUND OF THE INVENTION
[0002] 1. Field of the Invention
[0003] The present invention relates generally to synergistic
compositions that treat or inhibit pathological conditions
associated with tissue-specific activation of inflammation and to
methods of modulating inflammation in cells. More specifically, the
invention relates to composition comprising a fraction isolated or
derived from hops along with a synergist, such as rosemary, an
extract derived from rosemary, a compound derived from rosemary, a
triterpene species, a diterpene lactone species, and
tryptanthrin.
[0004] 2. Description of the Related Art
[0005] Cyclooxygenase (prostaglandin endoperoxide synthase, EC
1.14.991, COX) catalyzes the rate-limiting step in the metabolism
of arachidonic acid to prostaglandin H.sub.2 (PGH.sub.2), which is
further metabolized to various prostaglandins, prostacyclin and
thromboxane A2 (c.f. FIG. 1). In the early 1990s, it was
established that COX exists in two isoforms, commonly referred to
as COX-1 and COX-2. It was subsequently determined that the COX-1
and COX-2 proteins are derived from distinct genes that diverged
well before birds and mammals. Prostaglandins (PGs) generated via
the COX-1 and COX-2 pathways are identical molecules and therefore
have identical biological effects. COX-1 and COX-2, however, may
generate a unique pattern and variable amounts of eicosanoids;
therefore, relative differences in the activation of these isozymes
may result in quite dissimilar biological responses. Differences in
the tissue distribution and regulation of COX-1 and COX-2 are now
considered crucial for the beneficial as well as adverse effects of
COX inhibitors.
[0006] The generally held concept (COX dogma) is that COX-1 is
expressed constitutively in most tissues whereas COX-2 is the
inducible enzyme triggered by pro-inflammatory stimuli including
mitogens, cytokines and bacterial lipopolysaccharide (LPS) in cells
in vitro and in inflamed sites in vivo. Based primarily on such
differences in expression, COX-1 has been characterized as a
housekeeping enzyme and is thought to be involved in maintaining
physiological functions such as cytoprotection of the gastric
mucosa, regulation of renal blood flow, and control of platelet
aggregation. COX-2 is considered to mainly mediate inflammation,
although constitutive expression is found in brain, kidney and the
gastrointestinal tract. Therefore, it would be desirable to
down-regulate tissue-specific or cell-specific expression of
COX-2.
[0007] Arachidonic acid serves as the primary substrate for the
biosynthesis of all PGs. PGs are ubiquitous hormones that function
as both paracrine and autocrine mediators to affect a myriad of
physiological changes in the immediate cellular environment. The
varied physiological effects of PGs include inflammatory reactions
such as rheumatoid arthritis and osteoarthritis, blood pressure
control, platelet aggregation, induction of labor and aggravation
of pain and fever. The discovery 30 years ago that aspirin and
other non-steroidal analgesics inhibited PG production identified
PG synthesis as a target for drug development. There are at least
16 different PGs in nine different chemical classes, designated PGA
to PGI. PGs are part of a larger family of 20-carbon-containing
compounds called eicosanoids; they include prostacyclins,
thromboxanes, and leukotrienes. The array of PGs produced varies
depending on the downstream enzymatic machinery present in a
particular cell type. For example, endothelial cells produce
primarily PGI.sub.2, whereas platelets mainly produce
TXA.sub.2.
[0008] Prostaglandins (PG) are believed to play an important role
in maintenance of human gastric mucosal homeostasis. Current dogma
is that COX-1 is responsible for PG synthesis in normal gastric
mucosa in order to maintain mucosal homeostasis and that COX-2 is
expressed by normal gastric mucosa at low levels, with induction of
expression during ulcer healing, following endotoxin exposure or
cytokine stimulation. It now appears that both COX-1 and COX-2 have
important physiological roles in the normal gastric mucosa.
[0009] Compounds that inhibit the production of PGs by COX have
become important drugs in the control of pain and inflammation.
Collectively these agents are known as non-steroidal
anti-inflammatory drugs (NSAIDs) with their main indications being
osteoarthritis and rheumatoid arthritis. However, the use of
NSAIDs, and in particular aspirin, has been extended to prophylaxis
of cardiovascular disease. Over the last decade, considerable
effort has been devoted to developing new molecules that are direct
inhibitors of the enzymatic activity of COX-2, with the inference
that these compounds would be less irritating to the stomach with
chronic use. Therefore, it would be desirable to inhibit
inflammation response selectively in target cells.
[0010] U.S. patent application 2002/0086070A1 of Kuhrts entitled,
"ANTI-INFLAMMATORY AND CONNECTIVE TISSUE REPAIR FORMULATIONS"
describes a hops component that has an IC.sub.50-WHMA COX-2/COX-1
ratio ranging from about 0.23 to about 3.33. Example 1 of the
application describes a composition containing an extract obtained
through supercritical carbon dioxide extraction of whole hops
(CO.sub.2-extract) comprising 42% humulone.
[0011] U.S. Pat. No. 6,391,346 entitled, "ANTI-INFLAMMATORY,
SLEEP-PROMOTING HERBAL COMPOSITION AND METHOD OF USE" describes an
orally administered composition capable of reducing inflammation in
animals, while promoting sleep for such animals. The composition
contains hydroalcoholic extract of hops and supercritical carbon
dioxide extract of hops which are used to promote sleep.
[0012] An ideal formulation for the treatment of inflammation would
inhibit the induction and activity of COX-2 without inhibiting the
synthesis of PGE.sub.2 in gastric mucosal cells. However,
conventional non-steroidal anti-inflammatory drugs lack the
specificity of inhibiting COX-2 without affecting gastric PGE.sub.2
synthesis and are at risk to cause damages on the gastrointestinal
system, when used for extended periods. Indeed, even the newly
developed, anti-inflammatory drugs such as rofecoxib and celexocib
produce untoward gastric toxicity in the form of induced
spontaneous bleeding and delay of gastric ulcer healing.
[0013] Thus, it would be useful to identify a formulation of
compounds that would specifically inhibit or prevent the synthesis
of prostaglandins by COX-2 with little or no effect on synthesis of
PGE.sub.2 in the gastric mucosa. Such a formulation, which would be
useful for preserving the health of joint tissues, for treating
arthritis or other inflammatory conditions, has not previously been
discovered. The term "specific or- selective COX-2 inhibitor" was
coined to embrace compounds or mixtures of compounds that
selectively inhibit COX-2 over COX-1. However, while the
implication is that such a calculated selectivity will result in
lower gastric irritancy, unless the test materials are evaluated in
gastric cells, the term "selective COX-2 inhibitor" does not carry
assurance of safety to gastrointestinal cells. Only testing of
compound action in target tissues, inflammatory cells and gastric
mucosal cells, will identify those agents with low potential for
stomach irritation.
[0014] The major problem associated with ascertaining COX-2
selectivity (i.e. low gastric irritancy) is that differences in
assay methodology can have profound effects on the results
obtained. Depicted in Table 1 are the categories of the numerous in
vitro assays that have been developed for testing and comparing the
relative inhibitory activities of NSAID and natural compounds
against COX-1 and COX-2. These test systems can be classified into
three groups: (1) systems using animal enzymes, animal cells or
cell lines, (2) assays using human cell lines, or human platelets
and monocytes, and (3) currently evolving models using human cells
that are representative of the target cells for the
anti-inflammatory and adverse effects of NSAID and dietary
supplements. Generally, models using human cell lines or human
platelets and monocytes are the current standard and validated
target cell models have not been forthcoming. A human gastric cell
line capable of assessing potential for gastric irritancy is a
need. TABLE-US-00001 TABLE 1 Classification of test systems for in
vitro assays assessing COX-2 selectivity of anti-inflammatory
compounds.dagger. TEST SYSTEMS ANIMAL HUMAN TARGET Enzymes Enzymes
Human Gastric Mucosa Cells Cells Cells Human Chondrocytes Cell
lines Cell lines Human Synoviocytes OTHER SYSTEM VARIABLES 1.
Source of arachidonic acid - endogenous or exogenous; 2. Various
expression systems for gene replication of COX-1 and COX-2; 3. The
presence or absence of a COX-2 inducing agent; 4. COX-2 inducing
agents are administered at different concentrations and for
different periods of time; 5. Duration of incubation with the drug
or with arachidonic acid; 6. Variation in the protein concentration
in the medium. .dagger.Adapted from Pairet, M. and van Ryn, J.
(1998) Experimental models used to investigate the differential
inhibition of cyclooxygenase-1 and cyclooxygenase-2 by
non-steroidal anti-inflammatory drugs. Inflamm. Res 47, Supplement
2S93-S101 and incorporated herein by reference.
[0015] The enzymes used can be of animal or human origin, they can
be native or recombinant, and they can be used either as purified
enzymes, in microsomal preparations, or in whole-cell assays. Other
system variables include the source of arachidonic acid. PG
synthesis can be measured from endogenously released arachidonic
acid or exogenously added arachidonic acid. In the later case,
different concentrations are used in different laboratories.
[0016] Second, there are various expression systems for gene
replication of recombinant COX-1 and COX-2 enzymes. In addition,
the cells transfected with the Cox-1 or Cox-2 gene can be of
diverse origins, for instance, insect cell lines or COS cells.
Third, the absence or presence of a COX-2 inducing agent can vary.
Cells that are stably transfected with the recombinant enzymes
express this enzyme constitutively and no inducing agent is used.
This is in fundamental contrast with other cells in which COX-2 has
to be induced. Induction of COX-2 is commonly performed using
bacterial LPS or various cytokines such as interleukin-1.beta. or
tumor necrosis factor. Additionally, these endotoxins and cytokines
are administered at various concentrations.
[0017] Fourth, the duration of the incubation with the test agent,
the COX-2 inducing agent, or with arachidonic acid varies among
different laboratories. These differences can influence the
quantitative outcome of the study, because the inhibition of COX-2
is time dependent. Finally, the protein concentration of the medium
can vary; this is an issue for compounds that can bind avidly to
plasma proteins.
[0018] An ideal assay for COX-2 selectivity would have the
following characteristics: (1) whole cells should be used that
contain native human enzymes under normal physiological control
regarding expression; (2) the cells should also be target cells for
the anti-inflammatory and adverse effects of the compounds; (3)
COX-2 should be induced, thereby simulating an inflammatory
process, rather than being constitutively expressed; and (4) PG
synthesis should be measured from arachidonic acid released from
endogenous stores rather than from exogenously added arachidonic
acid.
[0019] Differences in methodology for can explain a dramatic
difference in the results obtained for COX inhibition. For example,
when assayed against the purified enzyme, ursolic acid exhibited an
IC.sub.50 of 130 .mu.M, far outside of possible physiologically
obtainable concentrations [Ringbom, T. et al. (1998) Ursolic acid
from Plantago major, a selective inhibitor of cyclooxygenase-2
catalyzed prostaglandin biosynthesis. J Nat Prod 61, 1212-1215]. In
the RAW 264.7 murine macrophage line, Suh et al. report an
IC.sub.50 for ursolic acid of approximately 40 .mu.M [Suh, N., et
al. (1998) Novel triterpenoids suppress inducible nitric oxide
synthase (iNOS) and inducible cyclooxygenase (COX-2) in mouse
macrophages. Cancer Res 58, 717-723]; and in phorbol 12-myristate
13-acetate stimulated human mammary cells, the approximate median
inhibitory concentration of ursolic acid was 3.0 .mu.M
[Subbaramaiah, K. et al. (2000) Ursolic acid inhibits
cyclooxygenase-2 transcription in human mammary epithelial cells.
Cancer Res 60, 2399-2404].
[0020] No laboratory has, as yet, developed an ideal assay for
COX-2 selectivity. The whole cell system most commonly used for Rx
and OTC products is the human whole blood assay developed by the
William Harvey Institute [Warner, T. D. et al. (1999) Nonsteroid
drug selectivities for cyclo-oxygenase-1 rather than
cyclo-oxygenase-2 are associated with human gastrointestinal
toxicity: a full in vitro analysis. Proc Natl Acad Sci U S A 96,
7563-7568]. To date, this assay format has developed more data
supporting clinical relevance than any other. However, new research
in the role of constitutive expression of COX-2 in normal gastric
mucosa necessitates revisiting the relevance of the use of
platelets to model COX-1 inhibition in the absence of COX-2. The
extrapolation of gastrotoxicity from platelet studies is no longer
on a sound molecular basis. The validation of a human gastric
mucosal cell line for establishing the potential target tissue
toxicity of cyclooxygenase inhibitors represents a critical need
for the development of safe and effective anti-inflammatory
agents.
[0021] Therefore, it would be useful to identify a composition that
would specifically inhibit or prevent the expression of COX-2
enzymatic activity in inflammatory cells, while having little or no
effect on PGE.sub.2 synthesis in gastric mucosal cells so that
these formulations could be used with no gastrointestinal upset.
Furthermore, such formulations should allow for healing of
pre-existing ulcerative conditions in the stomach.
SUMMARY OF THE INVENTION
[0022] Thus, it would be useful to identify a formulation of
compounds that would to modulate inflammatory response. Such a
formulation has widespread applications.
[0023] It would also be useful to identify a formulation of
compounds that would inhibit expression of COX-2, inhibit
prostaglandin synthesis selectively in target cells, or inhibit
inflammation response selectively in target cells. For example, it
would also be useful to identify a formulation of compounds that
would specifically inhibit or prevent the synthesis of
prostaglandins by COX-2 in inflammatory cells with little or no
effect on PGE.sub.2 synthesis in gastric mucosal cells. Such a
formulation, which would be useful for preserving the health of
joint tissues, for treating arthritis or other inflammatory
conditions, has not previously been discovered. Preferably, the
formulations have a median effective concentration for COX-2
inhibition in inflammatory cells that is minimally ten times
greater than the median effective concentration for the inhibition
of PGE.sub.2 synthesis in gastric cells. For example, if the median
inhibitory concentration for COX-2 of a test formulation was 0.2
.mu.g/mL in the murine macrophage RAW 264.7, the formulation would
not be considered to have low potential for gastric irritancy
unless the median inhibitory concentration for PGE.sub.2 synthesis
in gastric cells was equal to or greater than 2 .mu.g/mL.
[0024] A preferred embodiment comprises compositions containing at
least one fraction isolated or derived from hops (Humulus lupulus).
Examples of fractions isolated or derived from hops are alpha
acids, isoalpha acids, reduced isoalpha acids, tetra-hydroisoalpha
acids, hexa-hydroisoalpha acids, beta acids, and spent hops.
Preferred compounds of fractions isolated or derived from hops,
include, but are not limited to, humulone, cohumulone, adhumulone,
isohumulone, isocohumulone, isoadhumulone, dihydro-isohumulone,
dihydro-isocohumulone, dihydro-adhumulone, tetrahydro-isohumulone,
tetrahydro-isocohumulone, tetrahydro-adhumulone,
hexahydro-isohumulone, hexahydro-isocohumulone, and
hexahydro-adhumulone. Preferred compounds can also bear
substituents, such as halogens, ethers, and esters.
[0025] Other embodiments relate to combinations of components. One
embodiment relates to compositions that include, as a first
component, an active ingredient isolated or derived from an extract
of hops and as a second component at least one member selected from
the group consisting of rosemary (Rosmarinus officinalis L.), an
extract or compound derived from rosemary, a triterpene species or
derivatives or conjugates thereof, a diterpene lactone species or
derivatives or conjugates thereof, and tryptanthrin or conjugates
thereof. Another embodiment relates to compositions that include,
as a first component, tryptanthrin or conjugates thereof and as a
second component at least one member selected from the group
consisting of an active ingredient isolated or derived from an
extract of hops, rosemary, an extract or compound derived from
rosemary, a triterpene species or derivatives or conjugates
thereof, and a diterpene lactone species or derivatives or
conjugates thereof.
[0026] Preferred compositions can inhibit the inducibility or
activity of COX-2. Preferred compositions also can inhibit
prostaglandin synthesis selectively in target cells. Preferred
compositions also can inhibit inflammation response selectively in
target cells.
[0027] The compositions have widespread applications. Preferred
compositions can be useful for treating conditions, such as cancer,
autoimmune diseases, inflammatory diseases, neurological diseases.
Preferred compositions are also believed to be useful for treating
conditions, such as HIV-1 infections, rhinovirus infections, and
cardiovascular diseases.
[0028] Preferred compositions would be useful for, but not limited
to, the treatment of inflammation in a subject, and for treatment
of other inflammation-associated disorders, such as an analgesic in
the treatment of pain and headaches, or as an antipyretic for the
treatment of fever. Preferred compositions would be useful to treat
arthritis, including but not limited to rheumatoid arthritis,
spondyloathopathies, gouty arthritis, osteoarthritis, systemic
lupus erythematosis, and juvenile arthritis.
[0029] Preferred compositions would be useful in the treatment of
asthma, bronchitis, menstrual cramps, tendonitis, bursitis, and
skin-related conditions such as psoriasis, eczema, burns and
dermatitis. Preferred compositions also would be useful to treat
gastrointestinal conditions such as inflammatory bowel disease,
Crohn's disease, gastritis, irritable bowel syndrome and ulcerative
colitis and for the prevention or treatment of cancer such as
colorectal cancer.
[0030] Further, preferred compositions would be useful in treating
inflammation in such diseases as vascular diseases, migraine
headaches, periarteritis nodosa, thyroiditis, aplastic anemia,
Hodgkin's disease, sclerodma, rheumatic fever, type I diabetes,
myasthenia gravis, multiple sclerosis, sacoidosis, nephrotic
syndrome, Behchet's syndrome, polymyositis, gingivitis,
hypersensitivity, swelling occurring after injury, myocardial
ischemia, peridontal disease, fibromyalgia, atopic dermatitis,
insulitis and the like.
[0031] Additionally, preferred compositions would also be useful in
the treatment of ophthalmic diseases, such as retinopathies,
conjunctivitis, uveitis, ocular photophobia, and of acute injury to
the eye tissue. Preferred compositions would also be useful in the
treatment of pulmonary inflammation, such as that associated with
viral infections and cystic fibrosis.
[0032] Preferred compositions would also be useful for the
treatment of certain nervous system disorders such as cortical
dementias including Alzheimer's disease. As inhibitors of COX-2
mediated biosynthesis of PGE.sub.2 in inflammatory cells, these
compositions would also be useful in the treatment of allergic
rhinitis, respiratory distress syndrome, endotoxin shock syndrome,
atherosclerosis, and central nervous system damage resulting from
stroke, ischemia and trauma.
[0033] Preferred embodiments further provides a composition to
increase the rate at which glucosamine or chondrotin sulfate
function to normalize joint movement or reduce the symptoms of
osteoarthritis.
[0034] Preferred embodiments also provide for methods of
identifying compositions that would specifically inhibit or prevent
the synthesis of prostaglandins by COX-2 in inflammatory cells with
little or no effect on PGE.sub.2 synthesis in gastric mucosal
cells.
BRIEF DESCRIPTION OF THE DRAWINGS
[0035] FIG. 1 depicts the induction of cyclooxygenase-2 and the
metabolism of arachidonic acid to prostaglandins and other
eicosanoids by the cyclooxygenase enzymes. The action of
non-steroidal anti-inflammatory agents is through direct inhibition
of the cyclooxygenase enzymes.
[0036] FIG. 2 shows an outline of fractions and compounds that can
be obtained from hops.
[0037] FIG. 3 illustrates [A] the alpha-acid genus (AA) and
representative species humulone
(R.dbd.--CH.sub.2CH(CH.sub.3).sub.2), cohumulone (R.dbd.,
--CH(CH.sub.3).sub.2), and adhumulone
(R.dbd.--CH(CH.sub.3)CH.sub.2CH.sub.3); [B] the isoalpha acid genus
(IAA) and representative species isohumulone
(R.dbd.--CH.sub.2CH(CH.sub.3).sub.2), isocohumulone (R.dbd.,
--CH(CH.sub.3).sub.2), and isoadhumulone
(R.dbd.--CH(CH.sub.3)CH.sub.2CH.sub.3); [C] the reduced isomerized
isoalpha acid genus (RIAA) and representative species
dihydro-isohumulone (R.dbd.--CH.sub.2CH(CH.sub.3).sub.2)
dihydro-isocohumulone (R.dbd., --CH(CH.sub.3).sub.2), and
dihydro-adhumulone (R.dbd.--CH(CH.sub.3)CH.sub.2CH.sub.3); [D] the
tetra-hydroisoalpha acid genus (THIAA) and representative species
tetra-hydro-isohumulone (R.dbd.--CH.sub.2CH(CH.sub.3).sub.2),
tetra-hydro-isocohumulone ((R.dbd., --CH(CH.sub.3).sub.2), and
tetra-hydro-adhumulone (R.dbd.--CH(CH.sub.3)CH.sub.2CH.sub.3); [E]
and the hexa-hydroisoalpha acid (HHIAA) genus with representative
species hexa-hydro-isohumulone (R.dbd.--CH.sub.2CH(CH.sub.3).sub.2)
hexa-hydro-isocohumulone (R.dbd., --CH(CH.sub.3).sub.2), and
hexa-hydro-adhumulone (R.dbd.--CH(CH.sub.3)CH.sub.2CH.sub.3).
[0038] FIG. 4 illustrates the chemical structure of
tryptanthrin.
[0039] FIG. 5 illustrate the general chemical structures of the
triterpene genus [A] and ursolic acid [B] and oleanolic acid [C] as
a species within that genus.
[0040] FIG. 6 are representative immunoblots demonstrating
constitutive COX-1 and COX-2 expression in AGS human gastric
mucosal cells. The AGS human gastric cell line was cultured in
6-well plates at 37.degree. C. with 5% CO.sub.2 in a humidified
incubator for 24 hours. Cells were lysed on ice in lysis buffer and
protein concentration determined. Fifty .mu.g of cell lysate were
solubilized, fractionated on a 10% polyacrylamide gel containing
sodium dodecylsulfate (SDS), and transferred onto a nitrocellulose
membrane. The membranes were incubated in a blocking buffer and
then incubated with the respective primary antibody for 1 h at room
temperature. Following primary antibody incubation, the blots were
washed three times with Tris-buffered saline and then incubated
with the secondary antibody for 1 h. Protein bands were visualized
using enhanced chemiluminescence.
[0041] FIG. 7 [A] shows the percent inhibition of PGE.sub.2
synthesis in LPS-stimulated RAW 264.7 cells by plasma samples from
a human volunteer receiving 880 mg t.i.d. of a test hops derivative
formulation. White bars are means of raw data and dark bars are
those means computed with the elimination of outliers (never more
than two of the eight replicates). The gel capsules of the test
formulation contained 200 mg reduced isomerized alpha-acids, 200 mg
rosemary extract and 40 mg oleanolic acid. FIG. 7[B] is an estimate
of the plasma concentrations of test material at each post-dosing
time capable of inhibiting PGE.sub.2 synthesis in LPS-stimulated
RAW 264.7 cells assuming a constant 5:5:1 ratio of components.
[0042] FIG. 8 illustrates the induction of PGE.sub.2 synthesis by
mite allergen in A549 pulmonary cells treated for 24 hours.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
[0043] The present invention relates to the discovery that that a
supragenus of components isolated or derived from hops and other
compounds result in tissue-specific or cell-specific inhibition of
COX-2 expression. Importantly, these compounds are not believed to
directly inhibit COX-2 or other enzymes with the prostaglandin
synthesis pathway. Preferred embodiments provide compositions and
methods for inhibiting COX-2 expression, inhibiting prostanglandin
synthesis selectively in target tissues or cells, or inhibiting
inflammation response selectively in target tissues or cells.
[0044] A preferred embodiment comprises compositions containing
fractions or compounds isolated or derived from hops. Examples of
fractions isolated or derived from hops are alpha acids, isoalpha
acids, reduced isoalpha acids, tetra-hydroisoalpha acids,
hexa-hydroisoalpha acids, beta acids, and spent hops. Preferred
compounds of the fractions isolated or derived from hops can be
represented by a supragenus below: ##STR1## wherein R' is selected
from the group consisting of carbonyl, hydroxyl, OR, and OCOR,
wherein R is alkyl; wherein R'' is selected from the group
consisting of CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3; and wherein R, T, X, and Z are
independently selected from the group consisting of H, F, Cl, Br,
I, and .pi. orbital, with the proviso that if one of R, T, X, or Z
is a .pi. orbital, then the adjacent R, T, X, or Z is also a .pi.
orbital, thereby forming a double bond.
[0045] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR2##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0046] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR3##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0047] Examples of preferred compounds of an ingredient isolated or
derived from hops, include, but are not limited to, humulone,
cohumulone, adhumulone, isohumulone, isocohumulone, isoadhumulone,
dihydro-isohumulone, dihydro-isocohumulone, dihydro-adhumulone,
tetrahydro-isohumulone, tetrahydro-isocohumulone,
tetrahydro-adhumulone, hexahydro-isohumulone,
hexahydro-isocohumulone, and hexahydro-adhumulone. The preferred
compounds can bear substituents, as shown in the formula above.
[0048] Another embodiment comprises composition containing
tryptanthrin and conjugates thereof.
[0049] Other embodiments relate to combinations of components. The
preferred compositions can function synergistically to specifically
inhibit COX-2 expression, to inhibit prostaglandin synthesis
selectively in target cells, or to inhibit inflammation response
selectively in target cells.
[0050] One embodiment relates to compositions that include, as a
first component, an active ingredient isolated or derived from an
extract of hops and as a second component at least one member
selected from the group consisting of rosemary, an extract or
compound derived from rosemary, a triterpene species or derivatives
or conjugates thereof, a diterpene lactone species or derivatives
or conjugates thereof, and tryptanthrin or conjugates thereof.
Another embodiment relates to compositions that include, as a first
component, tryptanthrin or conjugates thereof and as a second
component at least one member selected from the group consisting of
an active ingredient isolated or derived from an extract of hops,
rosemary, an extract or compound derived from rosemary, a
triterpene species or derivatives or conjugates thereof, and a
diterpene lactone species or derivatives or conjugates thereof.
[0051] As used herein, the term "dietary supplement" refers to
compositions consumed to affect structural or functional changes in
physiology. The term "therapeutic composition" refers to any
compounds administered to treat or prevent a disease.
[0052] As used herein, the term "effective amount" means an amount
necessary to achieve a selected result. Such an amount can be
readily determined without undue experimentation by a person of
ordinary skill in the art.
[0053] As used herein, the term "substantial" means being largely
but not wholly that which is specified.
[0054] As used herein, the term "COX inhibitor" refers to a
composition of compounds that is capable of inhibiting the activity
or expression of COX-2 enzymes or is capable of inhibiting or
reducing the severity, including pain and swelling, of a severe
inflammatory response.
[0055] As used herein, the terms "derivatives" or a matter
"derived" refer to a chemical substance related structurally to
another substance and theoretically obtainable from it, i.e. a
substance that can be made from another substance. Derivatives can
include compounds obtained via a chemical reaction.
[0056] As used herein, the term "inflammatory cell" refers to those
cellular members of the immune system, for example B and T
lymphocytes, neutrophils or macrophages involved in synthesis of
prostaglandins in response to inflammatory signals such as
interleukins, tumor necrosis factor, bradykinin, histamine or
bacterial-derived components.
[0057] As used herein, the term "target cells" refers to that cell
population in which the inhibition of PGE.sub.2 or other
prostaglandin synthesis is desired, such as inflammatory cells,
tumor cells, or pulmonary cells. Alternatively, "non-target cells"
refers to that cell population in which the inhibition of PGE.sub.2
or other prostaglandin synthesis is not desired, such as the
gastric mucosal, neural or renal cells.
[0058] As used herein, the term "hop extract" refers to the solid
material resulting from (1) exposing a hops plant product to a
solvent, (2) separating the solvent from the hops plant products,
and (3) eliminating the solvent.
[0059] As used herein, the term "solvent" refers to a liquid of
aqueous or organic nature possessing the necessary characteristics
to extract solid material from the hop plant product. Examples of
solvents would include, but not limited to, water, steam,
superheated water, methanol, ethanol, hexane, chloroform, liquid
CO.sub.2, liquid N.sub.2 or any combinations of such materials.
[0060] As used herein, the term "CO.sub.2 extract" refers to the
solid material resulting from exposing a hops plant product to a
liquid or supercritical CO.sub.2 preparation followed by removing
the CO.sub.2.
[0061] As used herein, the term "spent hops" refers to the solid
and hydrophilic residue from extract of hops.
[0062] As used herein, the term "alpha acid" refers to compounds
refers to compounds collectively known as humulones and can be
isolated from hops plant products including, among others,
humulone, cohumulone, adhumulone, hulupone, and isoprehumulone.
[0063] As used herein, the term "isoalpha acid" refers to compounds
isolated from hops plant products and subsequently have been
isomerized. The isomerization of alpha acids can occur thermally,
such as boiling. Examples of isoalpha acids include, but are not
limited to, isohumulone, isocohumulone, and isoadhumulone.
[0064] As used herein, the term "reduced isoalpha acid" refers to
alpha acids isolated from hops plant product and subsequently have
been isomerized and reduced, including cis and trans forms.
Examples of reduced isoalpha acids (RIAA) include, but are not
limited to, dihydro-isohumulone, dihydro-isocohumulone, and
dihydro-adhumulone.
[0065] As used herein, the term "tetra-hydroisoalpha acid" refers
to a certain class of reduced isoalpha acid. Examples of
tetra-hydroisoalpha acid (THIAA) include, but are not limited to,
tetra-hydro-isohumulone, tetra-hydro-isocohumulone and
tetra-hydro-adhumulone.
[0066] As used herein, the term "hexa-hydroisoalpha acid" refers to
a certain class of reduced isoalpha acid. Examples of
hexa-hydroisoalpha acids (HHIAA) include, but are not limited to,
hexa-hydro-isohumulone, hexa-hydro-isocohumulone and
hexa-hydro-adhumulone.
[0067] As used herein, the term "beta-acid fraction" refers to
compounds collectively known as lupulones including, among others,
lupulone, colupulone, adlupulone, tetrahydroisohumulone, and
hexahydrocolupulone.
[0068] As used herein, the term "essential oil fraction" refers to
a complex mixture of components including, among others, myrcene,
humulene, beta-caryophyleen, undecane-2-on, and
2-methyl-but-3-en-ol.
[0069] As used herein, "conjugates" of compounds means compounds
covalently bound or conjugated to a member selected from the group
consisting of mono- or di-saccharides, amino acids, sulfates,
succinate, acetate, and glutathione. Preferably, the mono- or
di-saccharide is a member selected from the group consisting of
glucose, mannose, ribose, galactose, rhamnose, arabinose, maltose,
and fructose.
[0070] As used herein, the term "fats" refers to triacylglyerol
esters of fatty acids.
[0071] As used herein, the term "waxes" refers to triacylglycerol
ethers of or esters of extremely long chain (>25 carbons) fatty
alcohols or acids.
Hops
[0072] Hop extraction in one form or another goes back over 150
years to the early nineteenth century when extraction in water and
ethanol was first attempted. Even today an ethanol extract is
available in Europe, but by far the predominant extracts are
organic solvent extracts (hexane) and CO.sub.2 extracts
(supercritical and liquid). CO.sub.2 (typically at 60 bars pressure
and 50 to 10.degree. C.) is in a liquid state and is a relatively
mild, non-polar solvent highly specific for hop soft resins and
oils. Beyond the critical point, typically at 300 bars pressure and
60.degree. C., CO.sub.2 has the properties of both a gas and a
liquid and is a much stronger solvent. The composition of the
various extracts is compared in Table 2. TABLE-US-00002 TABLE 2 Hop
Extracts (Percent W/W) Super-Critical Component Hops Organic
Solvent CO.sub.2 Liquid CO.sub.2 Total resins 12-20 15-60 75-90
70-95 Alpha-acids 2-12 8-45 27-55 30-60 Beta-acids 2-10 8-20 23-33
15-45 Essential oils 0.5-1.5 0-5 1-5 2-10 Hard resins 2-4 2-10 5-11
None Tannins 4-10 0.5-5 0.1-5 None Waxes 1-5 1-20 4-13 0-10 Water
8-12 1-15 1-7 1-5
[0073] At its simplest, hop extraction involves milling, pelleting
and re-milling the hops to spread the lupulin, passing a solvent
through a packed column to collect the resin components and
finally, removal of the solvent to yield a whole or "pure" resin
extract.
[0074] The main organic extractants are strong solvents and in
addition to virtually all the lupulin components, they extract
plant pigments, cuticular waxes, water and water-soluble
materials.
[0075] Supercritical CO.sub.2 is more selective than the organic
solvents and extracts less of the tannins and waxes and less water
and hence water-soluble components. It does extract some of the
plant pigments like chlorophyll but rather less than the organic
solvents do. Liquid CO.sub.2 is the most selective solvent used
commercially for hops and hence produces the most pure whole resin
and oil extract. It extracts hardly the hard resins or tannins,
much lower levels of plant waxes, no plant pigments and less water
and water-soluble materials.
[0076] As a consequence of this selectivity and the milder solvent
properties, the absolute yield of liquid CO.sub.2, extract per unit
weight of hops is less than when using the other mentioned
solvents. Additionally, the yield of alpha acids with liquid
CO.sub.2 (89-93%) is lower than that of supercritical CO.sub.2
(91-94%) or the organic solvents (93-96%). Following extraction
there is the process of solvent removal, which for organic solvents
involves heating to cause volatilization. Despite this, trace
amounts of solvent do remain in the extract. The removal of
CO.sub.2, however, simply involves a release of pressure to
volatize the CO.sub.2.
[0077] As shown in FIG. 2, hops CO.sub.2 extracts can be
fractionated into components, including hops oils, beta acids, and
alpha acids. Hops oils include, but not limited to, humulene,
beta-caryophyllene, mycrene, farnescene, gamma-cadinene,
alpha-selinene, and alpha-cadinene. Beta acids include, but are not
limited to, lupulone, colupulone, adlupulone,
tetrahydroisohumulone, and hexahydrocolupulone, collectively known
as lupulones. Beta acids can be isomerized and reduced. Beta acids
are reduced to give tetra-beta acids. Alpha acids include, but are
not limited to, humulone, cohumulone, adhumulone, hulupone, and
isoprehumulone. Alpha acids can be isomerized to give isoalpha
acids. Iso-alpha acids can be reduced to give reduced-isoalpha
acids, tetra-hydroisoalpha acids, and hexa-hydroisoalpha acids.
[0078] A preferred embodiment comprises compositions containing
fractions or compounds isolated or derived from hops. Examples of
fractions isolated or derived from hops are alpha acids, isoalpha
acids, reduced isoalpha acids, tetra-hydroisoalpha acids,
hexa-hydroisoalpha acids, beta acids, and spent hops. Preferred
compounds of the fractions isolated or derived from hops can be
represented by a supragenus below: ##STR4## wherein R' is selected
from the group consisting of carbonyl, hydroxyl, OR, and OCOR,
wherein R is alkyl; wherein R'' is selected from the group
consisting of CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3; and wherein R, T, X, and Z are
independently selected from the group consisting of H, F, Cl, Br,
I, and .pi. orbital, with the proviso that if one of R, T, X, or Z
is a .pi. orbital, then the adjacent R, T, X, or Z is also a .pi.
orbital, thereby forming a double bond.
[0079] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR5##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0080] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR6##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0081] As shown in FIG. 3, examples of preferred compounds of an
ingredient isolated or derived from hops, include, but are not
limited to, humulone, cohumulone, adhumulone, isohumulone,
isocohumulone, isoadhumulone, dihydro-isohumulone,
dihydro-isocohumulone, dihydro-adhumulone, tetrahydro-isohumulone,
tetrahydro-isocohumulone, tetrahydro-adhumulone,
hexahydro-isohumulone, hexahydro-isocohumulone, and
hexahydro-adhumulone. The preferred compounds can bear
substituents, as shown in the formula above.
[0082] The identification of humulone from hops extract as an
inhibitor of bone resorption is reported in Tobe, H. et al. 1997.
(Bone resorption Inhibitors from hop extract. Biosci. Biotech.
Biochem 61(1)158-159.) Tobe et al. merely discloses the use of
humulone, cohumulone, adhumulone, isohumulone, isocohumulone, and
isoadhumulone for treating osteoporosis. Later studies by the same
group characterized the mechanism of action of humulone as
inhibition of COX-2 gene transcription following TNFalpha
stimulation of MC3T3, E1 cells [Yamamoto, K. 2000. Suppression of
cyclooxygenase-2 gene transcription by humulon of beer hop extract
studied with reference to the glucocorticoid receptor. FEBS Letters
465:103-106]. The authors concluded that the action of humulone
(also humulon) was similar to that of glucocorticoids, but that
humulone did not function through the glucocorticoid receptor.
While these results establish that humulone inhibits PGE.sub.2
synthesis in MC3T3 cells (osteoblasts) at the gene level, one
skilled in the art would not assume that these results would
necessarily occur in immune inflammatory cells or other cell lines.
Example 5 herein demonstrates the high degree of tissue selectivity
of hops compounds and derivatives.
[0083] Preferred embodiments provide compositions and methods for
inhibiting expression of COX-2, inhibiting synthesis of
prostaglandins selectively in target cells, and inhibiting
inflammatory response selectively in target cells. Preferred
methods comprise a step of administering to a mammal a composition
of the preferred embodiments. Preferred embodiments comprise a
fraction isolated or derived from hops. A certain composition
comprises alpha acids, isoalpha acids, reduced isoalpha acids,
tetra-hydroisoalpha acids, hexa-hydroisoalpha acids, beta acids, or
spent hops from hops extract or derivatives thereof. Preferred
compounds of the fractions isolated or derived from hops can be
represented by a supragenus below: ##STR7## wherein R' is selected
from the group consisting of carbonyl, hydroxyl, OR, and OCOR,
wherein R is alkyl; wherein R'' is selected from the group
consisting of CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3; and wherein R, T, X, and Z are
independently selected from the group consisting of H, F, Cl, Br, I
and .pi. orbital, with the proviso that if one of R, T, X, or Z is
a .pi. orbital, then the adjacent R, T, X, or Z is also a .pi.
orbital, thereby forming a double bond. Other preferred compounds
of the fractions isolated or derived from hops can be represented
by a genus below: ##STR8## wherein R' is selected from the group
consisting of carbonyl, hydroxyl, OR, and OCOR, wherein R is alkyl;
and wherein R'' is selected from the group consisting of
CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3. Other preferred compounds of the
fractions isolated or derived from hops can be represented by a
genus below: ##STR9## wherein R' is selected from the group
consisting of carbonyl, hydroxyl, OR, and OCOR, wherein R is alkyl;
and wherein R'' is selected from the group consisting of
CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3. The preferred embodiments contemplate
compositions comprising beta acids or isomerized or reduced beta
acids. Preferably, the alpha acid, isoalpha acid, reduced isoalpha
acid, tetra-hydroisoalpha acid, hexa-hydroisoalpha acid, beta acid,
or spent hops of the preferred embodiments is made from hops
extract. More preferably, the alpha acid, isoalpha acid, reduced
isoalpha acid, tetra-hydroisoalpha acid, hexa-hydroisoalpha acid,
beta acid, or spent hops of the preferred embodiments is made from
CO.sub.2 extract of hops. Tryptanthrin
[0084] Preferred embodiments can provide compositions and methods
for inhibiting expression of COX-2, inhibiting synthesis of
prostaglandins selectively in target cells, and inhibiting
inflammatory response selectively in target cells. Preferred
methods comprise a step of administering to a mammal a composition
of the preferred embodiments. A certain composition comprises
tryptanthrin and conjugates thereof.
[0085] Depicted in FIG. 4, tryptanthrin is a natural compound found
in certain herbs, such as Polygonum tinctorium and Isatis
tinctoria. In traditional Chinese medicine this herb is known as Da
Qing Ye or Qing Dai. The herb has demonstrated antibacterial and
antiviral activity. It has antipyretic, anti-inflammatory and
choleretic properties. Increased phagocytic activity of leukocytes
and relaxation of intestinal smooth muscle are additional
properties of Qing Dai.
Rosemary
[0086] Certain of preferred embodiments also include delivering an
effective amount of rosemary, rosemary extract, or compounds
derived from rosemary with the fraction isolated or derived from
hops or tryptanthrin. Preferred additions include, but are not
limited to, rosemary, rosemary extract, or those compounds known to
be found in rosemary or extracts of rosemary. These include
1,8-cineole, 19-alpha-hydroxyursolic acid,
2-.beta.-hydroxyoleanolic acid, 3-acetyloleanolic acid,
3-O-acetylursolic acid, 6-methoxy-luteolin-7-glucoside,
6-methoxyluteolin, 6-methoxyluteolin-7-glucoside,
methoxyluteolin-7-methylether, 7-ethoxy-rosmanol,
7-methoxy-rosmanol, alpha-amyrin, alpha-humulene,
alpha-hydroxyhydrocaffeic acid, alpha-pinene, alpha-terpinene,
alpha-terpinenyl acetate, alpha-terpineol, alpha-thujone, apigenin,
apigenin-7-glucoside, curcumene, benzyl-alcohol, .beta.-amyrenone,
.beta.-amyrin, .beta.-elemene, .beta.-pinene, betulin**, betulinic
acid**, borneol, bornyl-acetate, caffeic acid, camphene, camphor,
carnosic acid**, carnosol**, carvacrol**, carvone, caryophyllene,
caryophyllene-oxide, chlorogenic acid**, diosmetin**,
gamma-terpinene, hesperidin, isoborneol, limonene*, luteolin*,
luteolin-3'-O-(3''-O-acetyl)-.beta.-D-glucuronide,
luteolin-3'-O-(4''-O-acetyl)-.beta.-D-glucuronide,
luteolin-3'-O-.beta.-D-glucuronide, luteolin-7-glucoside,
methyl-eugenol, myrcene, neo-chlorogenic acid, nepetin, octanoic
acid, oleanolic acid, p-cymene, piperitenone, rosmanol, rosmaric
acid, rosmaricine, rosmaridiphenol, rosemarinic acid, rosmarinol,
rosmariquinone, sabinene, sabinyl acetate, salicylates, salicylic
acid-2-B-D-glucoside, squalene, terpinen-4-ol, terpinolene, thymol,
trans-anethole, trans-carveol, ursolic acid, verbenone, and
zingiberene. Of the species listed, those containing at least one
asterisk (*) are preferred and those containing two asterisks (**)
are particularly preferred.
Triterpenes and Diterpene Lactones
[0087] Certain of preferred embodiments also include delivering an
effective amount of a triterpene species or diterpene lactone
species with the fraction isolated or derived from hops or
tryptanthrin. Preferred triterpenes include oleanolic acid, and
ursolic acid. Both ursolic and oleanolic acid are found in a wide
variety of botanicals. Diterpene lactones, such as andrographolide,
can be obtained from Andrographis paniculata.
[0088] Diterpene lactone species, such as andrographolide, and
triterpenes, such as ursolic acid and oleanolic acid, are commonly
found in plants and are used for their anti-inflammatory
properties. The anti-inflammatory effects of these compounds have
been described in the literature since 1960. Their mechanism of
action is believed to be due (i) to the inhibition of histamine
release from mast cells or (ii) to the inhibition of lipoxygenase
and cyclooxygenase activity thereby reducing the synthesis of
inflammatory factors produced during the arachidonic acid cascade.
Since andrographolide and oleanolic acid have been found to promote
the healing of stomach ulcers, it is unlikely that the
cyclooxygenase activity that is inhibited is COX-1. Also,
andrographolide and oleanolic acid are potent antioxidants, capable
of inhibiting the generation of reactive oxygen intermediates and
restoring tissue glutathione levels following stress.
[0089] For example, botanical sources for ursolic acid can be
selected from the group consisting of Adina piluifera, Agrimonia
eupatoria, Arbutus unedo, Arctostaphylos uva-ursi, Artocarpus
heterophyllus, Catalpa bignoniodes, Catharanthus roseus, Chimaphila
umbellata, Cornus florida, Cornus officinalis, Crataegus cuneata,
Crataegus laevigata, Crataegus pinnatifida, Cryptostegia
grandifolia, Elaeagnus pungens, Eriobotrya japonica, Eucalyptus
citriodora, Forsythia suspensa, Gaultheria fragrantissima, Glechoma
hederacea, Hedyotis diffusa, Helichrysum angustifolium, Humulus
lupulus, Hyssopus officinalis, Ilex paraguariensis, Lavandula
angustifolia, Lavandula latifolia, Leonurus cardiaca, Ligustrum
japonicum, Limonia acidissima, Lycopus europeus, Malus domestica,
Marubium vulgare, Melaleuca leucadendra, Melissa officinalis,
Mentha spicata, Mentha x rotundifolia, Monarda didyma, Nerium
oleander, Ocimum basilicum, Ocimum basilicum, Ocimum basilicum,
Ocimum baslicum, Ocimum canum, Origanum majorana, Origanum vulgare,
Plantago asiatica, Plantago major, Plectranthus amboinicus, Prunell
vulgaris, Prunella vulgaris, Prunus cerasus, Prunus laurocerasus,
Prunus persica, Prunus serotina spp serotina, Psidium guajava,
Punica granatum, Pyrus communis, Rhododendron dauricum,
Rhododendron ferrugineum, Rhododendron ponticum, Rosmarinus
officinalis, Rubus fruticosus, Salvia officinalis, Salvia sclarea,
Salvia triloba, Sambucus nigra, Sanguisorba officinalis, Satureja
hortensis, Satureja montana, Sorbus aucubaria, Syringa vulgaris,
Teucrium chamaedrys Teucrium polium, Teucrium spp, Thevetia
peruviana, Thymus serpyllum, Thymus vulgaris, Uncaria tomentosa,
Vaccinium corymobosum, Vaccinium myrtillus, Vaccinium vitis idaea,
Verbena officinalis, Viburnum opulus var. opulus, Viburnum
prunifolium, Vinca minor and Zizyphus jujuba.
[0090] Similarly, oleanolic acid is found in Achyranthes aspera,
Achyranthes bidentiata, Adina piluifera, Ajpocynum cannabinum,
Akebia quinata, Allium cepa, Allium sativum, Arctostaphylos
uva-ursi, Calendula officinalis, Catharanthus roseus, Centaurium
erythraea, Chenopodium album, Citrullus colocynthis, Cnicus
benedictus, Cornus officinalis, Crataegus pinnatifida Cyperus
rotundus, Daemonorops draco, Diospyros kaki, Elaeagnus pungens,
Eleutherococcus senticosus, Eriobotrya japonica, Eugenia
caryophyllata, Forsythia suspensa, Glechoma hederacea, Harpagophtum
procumbens, Hedera helix,, Hedyotis diffusa, Helianthus annuus,
Hemsleys amabilis, Humulus lupulus, Hyssopus officinalis, Ilex
rotunda, Lavandula latifolia, Leonurus cardiaca, Ligustrum
japonicum, Ligustrum lucidum, Liquidambar orientalis, Liquidambar
styraciflua, Loranthus parasiticus, Luffa aegyptiaca, Melaleuca
leucadendra, Melissa officinalis, Mentha spicata, Mentha x
rotundifolia, Momordica cochinchinensis, Myristica fragrans,
Myroxylon balsamum, Nerium oleander, Ocimum suave, Ociumum
basilicum, Olea europaea, Origanum majorana, Origanum vulgare,
Paederia scandens, Panax ginseng, Panax japonicus, Panax
quinquefolius, Patrinia scabiosaefolia, Phytolacca americana,
Plantago major, Plectranthus amboinicus, Prunella vulgaris, Prunus
cerasus, Psidium guajava, Pulsatilla chinenisis, Quisqualis indica,
Rosmarinus officinalis, Salvaia officinalis, Salvia sclarea, Salvia
triloba, Sambucus nigra, Satureja hortensis, Satureja montana,
Swertia chinensis, Swertia diluta, Swertia mileensis, Syzygium
aromaticum, Thymus serpyllum, Thymus vulgaris, Trachycarpus
fortunei, Uncaria tomentosa, Vaccinium corymbosum, Vaccinium
myrtillus, Viburnum prunifolium, Viscum album, Vitis vinifera, or
Zizyphus jujuba.
[0091] The preferred botanical sources for ursolic acid is a member
selected from the group consisting of Ligustrum japonicum, Plantago
asiatica, Plantago major, Prunus species, Uncaria tomentosa,
Zizyphus jujuba, Cornus officinalis, Eucalyptus citriodora,
Forsythia suspensa, Lavandula latifolia, Malus domestica, Nerium
oleander, Ocimum baslicum, Punica granatum, Pyrus communis,
Rosmarinus officinalis, Salvia triloba, Sorbus aucubaria, Vaccinium
myrtillus, Vaccinium vitis-idaea, and Viburnum opulus var. opulus.
The most preferred botanical sources for ursolic acid is a member
selected from the group consisting of Ligustrum japonicum, Plantago
asiatica, Plantago major, Prunus species, Uncaria tomentosa, and
Zizyphus jujuba.
[0092] The preferred botanical source for oleanolic acid is a
member selected from the group consisting of Eleutherococcus
senticosus, Ligustrum japonicum, Ligustrum lucidum, Panax ginseng,
Panax japonicus, Panax quinquefolius, Plantago major, Prunella
vulgaris, Vitis vinifera, Zizyphus jujuba, Achyranthes bidentiata,
Allium cepa, Allium sativum, Cornus officinalis, Daemonorops draco,
Forsythia suspensa, Prunus cerasus, Quisqualis indica, Rosmarinus
officinalis, Salvia triloba, Syzygium aromaticum, Thymus vulgaris,
Uncaria tomentosa, Vaccinium corymbosum, and Vaccinium myrtillus.
The most preferred botanical source for oleanolic acid is a member
selected from the group consisting of Eleutherococcus senticosus,
Ligustrum japonicum, Ligustrum lucidum, Panax ginseng, Panax
japonicus, Panax quinquefolius, Plantago major, Prunella vulgaris
Vitis vinifera and Zizyphus jujuba.
[0093] FIG. 5 illustrate the general chemical structures of the
triterpene genus and ursolic acid and oleanolic acid as a species
within that genus. Representative terpenoids within the genus are
18-a-glycyrrhetinic acid**, 18-13-glycyrrhetinic acid**,
2-a-3-a-dihydrooxyurs-12-3n-28-onic acid*, 3-a-hydroxyursolic
acid*, 3-oxo-ursolic acid*, betulin**, betulinic acid**,
celastrol*, eburicoic acid, friedelin*, glycyrrhizin, gypsogenin,
oleanolic acid**, oleanolic acid-3-acetate, pachymic acid,
pinicolic acid, sophoradiol, soyasapogenol A, soyasapogenol B,
tripterin**, triptophenolide*, tumulosic acid, ursolic acid**,
ursolic acid-3-acetate, uvaol*, and .beta.-sitosterol. Of the
species listed, those containing at least one asterisk (*) are
preferred and those containing two asterisks (**) are particularly
preferred.
[0094] Examples of diterpene lactone species include, but is not
limited to, andrographolide, dehydroandrographolide,
deoxyandrographolide, neoandrographolide, selenoandrographolide,
homoandrographolide, andrographan, amdrographon, andrographosterin,
14-deoxy- 11-oxoandrographolide, 14-deoxy-11,
12-didehydroandrographolide, andrographiside, and edelin
lactone.
Compositions and Synergistic Combinations
[0095] Preferred compositions can function to specifically inhibit
COX-2 expression, to inhibit prostaglandin synthesis selectively in
target cells, or to inhibit inflammation response selectively in
target cells. Preferred embodiments include compositions containing
fractions or compounds isolated or derived from hops or
compositions containing tryptanthrin and conjugates thereof.
[0096] A preferred embodiment comprises compositions containing
fractions or compounds isolated or derived from hops. Examples of
fractions isolated or derived from hops are alpha acids, isoalpha
acids, reduced isoalpha acids, tetra-hydroisoalpha acids,
hexa-hydroisoalpha acids, beta acids, and spent hops. Preferred
compounds of the fractions isolated or derived from hops can be
represented by a supragenus below: ##STR10## wherein R' is selected
from the group consisting of carbonyl, hydroxyl, OR, and OCOR,
wherein R is alkyl; wherein R'' is selected from the group
consisting of CH(CH.sub.3).sub.2, CH.sub.2CH(CH.sub.3).sub.2, and
CH(CH.sub.3)CH.sub.2CH.sub.3; and wherein R, T, X, and Z are
independently selected from the group consisting of H, F, Cl, Br, I
and .pi. orbital, with the proviso that if one of R, T, X, or Z is
a .pi. orbital, then the adjacent R, T, X, or Z is also a .pi.
orbital, thereby forming a double bond.
[0097] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR11##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0098] Other preferred compounds of the fractions isolated or
derived from hops can be represented by a genus below: ##STR12##
wherein R' is selected from the group consisting of carbonyl,
hydroxyl, OR, and OCOR, wherein R is alkyl; and wherein R'' is
selected from the group consisting of CH(CH.sub.3).sub.2,
CH.sub.2CH(CH.sub.3).sub.2, and CH(CH.sub.3)CH.sub.2CH.sub.3.
[0099] Examples of preferred compounds of an ingredient isolated or
derived from hops, include, but are not limited to, humulone,
cohumulone, adhumulone, isohumulone, isocohumulone, isoadhumulone,
dihydro-isohumulone, dihydro-isocohumulone, dihydro-adhumulone,
tetrahydro-isohumulone, tetrahydro-isocohumulone,
tetrahydro-adhumulone, hexahydro-isohumulone,
hexahydro-isocohumulone, and hexahydro-adhumulone. The preferred
compounds can bear substituents, as shown in the formula above.
[0100] Another embodiment comprises composition containing
tryptanthrin and conjugates thereof.
[0101] Other embodiments relate to combinations of components.
Preferred compositions can function synergistically to specifically
inhibit COX-2 expression, to inhibit prostaglandin synthesis
selectively in target cells, or to inhibit inflammation response
selectively in target cells.
[0102] One embodiment relates to compositions that include, as a
first component, an active ingredient isolated or derived from an
extract of hops and as a second component at least one member
selected from the group consisting of rosemary, an extract or
compound derived from rosemary, a triterpene species or derivatives
or conjugates thereof, a diterpene lactone species or derivatives
or conjugates thereof, and tryptanthrin or conjugates thereof.
Another embodiment relates to compositions that include, as a first
component, tryptanthrin or conjugates thereof and as a second
component at least one member selected from the group consisting of
an active ingredient isolated or derived from an extract of hops,
rosemary, an extract or compound derived from rosemary, a
triterpene species or derivatives or conjugates thereof, a
diterpene lactone species or derivatives or conjugates thereof.
Dosage
[0103] The selected dosage level will depend upon activity of the
particular composition, the route of administration, the severity
of the condition being treated or prevented, and the condition and
prior medical history of the patient being treated. However, it is
within the skill of the art to start doses of the composition at
levels lower than required to achieve the desired therapeutic
effect and to gradually increase the dosage until the desired
effect is achieved. If desired, the effective daily dose may be
divided into multiple doses for purposes of administration, e.g.,
two to four separate doses per day. It will be understood, however,
that the specific dose level for any particular patient will depend
upon a variety of factors including body.. weight, general health,
diet, time and route of administration, combination with other
compositions and the severity of the particular condition being
treated or prevented.
[0104] Preferred embodiments include delivering an effective amount
of hops fractions, hops compounds, or hops derivatives alone or
with in combination with other active ingredients. Preferably, a
daily dose of preferred compositions would be formulated to deliver
about 0.5 to 10,000 mg of alpha acid, isoalpha acid, reduced
isoalpha acid, tetra-hydroisoalpha acid, hexa-hydroisoalpha acid,
beta acid, or spent hops per day. More preferably, an effective
daily dose of preferred compositions would be formulated to deliver
about 50 to 7500 mg of alpha acids, isoalpha acid, reduced isoalpha
acid, tetra-hydroisoalpha acid, hexa-hydroisoalpha acid, beta acid,
or spent hops per day. Preferably, the effective daily dose is
administered once or twice a day. A certain embodiment provides a
composition comprising about 0.5 to 800 mg of isoalpha acid or
reduced isoalpha acid, more preferably about 50 to 400 mg of
isoalpha acid or reduced isoalpha acid per day. Another certain
embodiment provides a composition comprising about 10 to 3000 mg of
reduced isoalpha acid, tetra-hydroisoalpha acid, or
hexa-hydroisoalpha acid per day, more preferably about 50 to 2000
mg of reduced isoalpha acid, tetra-hydroisoalpha acid, or
hexa-hydroisoalpha acid per day. Yet another certain embodiment
provides a composition comprising about 50 to 7500 mg of spent hops
per day, preferably about 100 to 6000 mg of spent hops per day.
[0105] Preferred embodiments include delivering an effective amount
of tryptanthrin or conjugates thereof alone or with in combination
with other active ingredients. Preferably, a daily dose of
preferred compositions would be formulated to deliver about 0.0005
to 50 mg tryptanthrin/kg body weight per day. More preferably, an
effective daily dose of preferred compositions would be formulated
to deliver about 0.01 to 10 mg tryptanthrin/kg body weight per day.
Preferably, a daily dose of preferred compositions would be
formulated to deliver about 0.035 to 3500 mg of tryptanthrin per
day. More preferably, an effective daily dose of preferred
composition would be formulated to deliver about 0.7 to 700 mg of
tryptanthrin per day. Preferably, the effective daily dose is
administered once or twice a day.
[0106] Preferred embodiments include delivering an effective amount
of rosemary or an extract or compound derived from rosemary in
combination with other active ingredients. Preferably, a daily dose
of preferred compositions would be formulated to deliver about 0.5
to 5000 mg of rosemary, an extract of rosemary, or rosemary-derived
compound per day. More preferably, an effective daily dose of
preferred composition would be formulated to deliver about 5 to
2000 mg of rosemary, an extract of rosemary, or rosemary-derived
compound per day. Preferably, the effective daily dose is
administered once or twice a day. A certain embodiment provides a
composition comprising about 75 mg of rosemary extract or
rosemary-derived compound or derivative, to be administered once or
twice a day.
[0107] Preferred embodiments include delivering an effective amount
of a triterpene or diterpene lactone species or derivatives or
conjugates thereof in combination with other active ingredients.
Preferably, a daily dose of preferred compositions would be
formulated to deliver about 0.0005 to 50 mg triterpene or diterpene
lactone/kg body weight per day. More preferably, an effective daily
dose of preferred compositions would be formulated to deliver about
0.01 to 10 mg triterpene or diterpene lactone/kg body weight per
day. Preferably, a daily dose of preferred compositions would be
formulated to deliver about 0.035 to 3500 mg of triterpene or
diterpene lactone species per day. More preferably, an effective
daily dose of preferred composition would be formulated to deliver
about 0.7 to 700 mg of triterpene or diterpene lactone species per
day. Preferably, the effective daily dose is administered once or
twice a day.
[0108] Preferably, an embodiment provides a composition containing
an extract of rosemary and a triterpene, such as oleanolic acid,
along with an active ingredient, such as a fraction isolated or
derived from hops or tryptanthrin or conjugate thereof. Preferably,
an embodiment provides a composition comprising about 0.01 to 500
mg of rosemary extract and about 0.01 to 500 mg of oleanolic acid.
Preferably, an embodiment provides a composition capable of
producing concentrations in target tissues of 0.1 to 10 .mu.g/g
tissue of rosemary extract and about 0.1 to 25 .mu.g/g tissue of
oleanolic acid.
[0109] A composition of preferred embodiments for topical
application would contain about 0.001 to 10 weight percent,
preferably about 0.1 to 1 weight percent of a hops extract
component or derivative or tryptanthrin or conjugate thereof.
Preferred embodiments would produce serum concentrations in the
ranges of about 0.0001 to 10 .mu.M, preferably about 0.01 to 1
.mu.M of a fraction isolated or derived from hops or tryptanthrin
or conjugate thereof. The preferred embodiments for topical
application can further comprise an additional ingredient selected
from rosemary, an extract or compound derived from rosemary, a
triterpene species or derivatives or conjugates thereof, a
diterpene lactone species or derivatives or conjugates thereof, a
fraction isolated or derived from hops or tryptanthrin or
conjugates thereof, at concentrations of each component of 0.001 to
10 weight percent, preferably 0.1 to 1 weight percent. Preferred
embodiments would produce serum concentrations in the ranges of
about 0.001 to 50 .mu.M, preferably about 0.1 .mu.M to 5 .mu.M of
the additional ingredient.
[0110] A certain composition comprises a first component selected
from a fraction isolated or derived from hops and a second
component comprising an extract or compound derived from rosemary,
an extract or compound derived from rosemary, a triterpene species
or derivatives or conjugates thereof, a diterpene lactone species
or derivatives or conjugates thereof, or tryptanthrin or conjugates
thereof Preferably, the weight ratio of the first component, i.e. a
fraction isolated or derived from hops to the second component,
i.e. an extract or compound derived from rosemary, an extract or
compound derived from rosemary, a triterpene species or derivatives
or conjugates thereof, a diterpene lactone species or derivatives
or conjugates thereof, or tryptanthrin or conjugates thereof, is
within a range of about 100:1 to about 1:100; preferably about 50:1
to about 1:50; more preferably about 10:1 to about 1:10.
[0111] A certain composition comprises a first component of
tryptanthrin and conjugates thereof, and a second component
comprising hops fraction, hops compound, hops derivative, rosemary,
an extract or compound derived from rosemary, a triterpene species
or derivatives or conjugates thereof, or a diterpene lactone
species or derivatives or conjugates thereof. Preferably, the
weight ratio of the first component, i.e. tryptanthrin and
conjugates thereof, to the second component, i.e. hops fraction,
hops compound, hops derivative, rosemary, an extract or compound
derived from rosemary, a triterpene species or derivatives or
conjugates thereof, or a diterpene lactone species or derivatives
or conjugates thereof, is within a range of about 100:1 to about
1:100; preferably about 50:1 to about 1:50; more preferably about
10:1 to about 1:10; even more preferably about 1:1.
Applications of Preferred Compositions
[0112] As stated previously, the generally held concept (COX dogma)
is that COX-1 is expressed constitutively in most tissues whereas
COX-2 is the inducible enzyme triggered by pro-inflammatory stimuli
including mitogens, cytokines and bacterial lipopolysaccharide
(LPS) in cells in vitro and in inflamed sites in vivo. Based
primarily on such differences in expression, COX-1 has been
characterized as a housekeeping enzyme and is thought to be
involved in maintaining physiological functions such as
cytoprotection of the gastric mucosa, regulation of renal blood
flow, and control of platelet aggregation. COX-2 is considered to
mainly mediate inflammation, although constitutive expression is
found in brain, kidney and the gastrointestinal tract. Therefore,
it would be desirable to down-regulate expression of COX-2
tissue-specifically or cell-specifically. Examples of target cells
include, but are not limited to, inflammatory cells, pulmonary
cells, and tumor cells. Examples of nontarget cells include, but
are not limited to, gastric mucosal, neural, and renal cells.
[0113] The compositions have widespread applications. Preferred
compositions can be useful for treating conditions, such as cancer,
autoimmune diseases, inflammatory diseases, neurological diseases.
Preferred compositions are also believed to be useful for treating
conditions, such as HIV-1 infections, rhinovirus infections, and
cardiovascular diseases.
[0114] Preferred embodiments would be useful for, but not limited
to a number of inflammatory conditions. Thus, preferred embodiments
include treatment of inflammation in a subject, and treatment of
other inflammation-associated disorders, such as, as an analgesic
in the treatment of pain and headaches, or as an antipyretic for
the treatment of fever. Additional examples of such preferred
embodiments would be useful to treat arthritis, including but not
limited to rheumatoid arthritis, spondyloathopathies, gouty
arthritis, osteoarthritis, systemic lupus erythematosis, and
juvenile arthritis. Such preferred embodiments would be useful in
the treatment of asthma, bronchitis, menstrual cramps, tendonitis,
bursitis, and skin related conditions such as psoriasis, eczema,
burns and dermatitis. Preferred embodiments also would be useful to
treat gastrointestinal conditions such as inflammatory bowel
disease, Crohn's disease, gastritis, irritable bowel syndrome and
ulcerative colitis and for the prevention or treatment of cancer
such as colorectal cancer. Preferred embodiments would be useful in
treating inflammation in such diseases as vascular diseases,
migraine headaches, periarteritis nodosa, thyroiditis, aplastic
anemia, Hodgkin's disease, sclerodma, rheumatic fever, type I
diabetes, myasthenia gravis, multiple sclerosis, sacoidosis,
nephrotic syndrome, Behchet's syndrome, polymyositis, gingivitis,
hypersensitivity, swelling occurring after injury, myocardial
ischemia and the like.
[0115] Preferred embodiments would also be useful in the treatment
of ophthalmic diseases, such as retinopathies, conjunctivitis,
uveitis, ocular photophobia, and of acute injury to the eye tissue.
Preferred embodiments would also be useful in the treatment of
pulmonary inflammation, such as that associated with viral
infections and cystic fibrosis. Preferred embodiments would also be
useful in the treatment of asthma. Preferred embodiments would also
be useful for the treatment of certain nervous system disorders
such as cortical dementias including Alzheimer's disease. Preferred
embodiments are useful as anti-inflammatory agents, such as for the
treatment of arthritis, with the additional benefit of having
significantly less harmful side effects. As inhibitors of COX-2
mediated biosynthesis of PGE.sub.2, these compositions would also
be useful in the treatment of allergic rhinitis, respiratory
distress syndrome, endotoxin shock syndrome, atherosclerosis, and
central nervous system damage resulting from stroke, ischemia and
trauma. The preferred embodiments would also be useful for the
treatment of fibromyalgia.
[0116] Since COX-2 can also play a role in the regulation of
osteoblastic function, preferred embodiments can also be useful for
treating and preventing osteoporosis. Kanematsu et al. (J Bone
Miner Res 1997 November;12(11):1789-96.) discloses that interleukin
1 (IL-1) and tumor necrosis factor alpha (TNF-alpha) have been
implicated in the pathogenesis of osteoporosis. These
proinflammatory cytokines induce both COX-2 and nitric oxide
synthase (iNOS) with the release of PGE.sub.2 and NO, respectively.
They determined the interaction between COX and NOS pathways and
their role in the regulation of osteoblastic function in MC3T3-E1
cells.
[0117] According to preferred embodiments, the animal may be a
member selected from the group consisting of humans, non-human
primates, dogs, cats, birds, horses, ruminants or other warm
blooded animals. Preferred embodiments are directed primarily to
the treatment of human beings. Administration can be by any method
available to the skilled artisan, for example, by oral, topical,
transdermal, transmucosal, or parenteral routes.
[0118] Besides being useful for human treatment, preferred
embodiments are also useful for treatment of other animals,
including horses, dogs, cats, birds, sheep, pigs, etc. A certain
formulation for the treatment of inflammation would inhibit the
induction and activity of COX-2 with little effect on the synthesis
of PGE.sub.2 in the gastric mucosa. Historically, the NSAIDs used
for treatment of inflammation lacked the specificity of inhibiting
COX-2 without affecting PGE.sub.2 synthesis in gastric mucosal
cells. Therefore, these drugs irritated and damaged the
gastrointestinal system when used for extended periods.
Formulations
[0119] Preferred compositions can be administered in the form of a
dietary supplement or therapeutic composition. The compositions may
be administered orally, topically, transdermally, transmucosally,
parenterally, etc., in appropriate dosage units, as desired.
[0120] Preferred compositions for dietary application may include
various additives such as other natural components of intermediary
metabolism, vitamins and minerals, as well as inert ingredients
such as talc and magnesium stearate that are standard excipients in
the manufacture of tablets and capsules. For example, one
embodiment comprises active ingredients of preferred compositions
in combination with glucosamine or chondrotin sulfate.
[0121] As used herein, "pharmaceutically acceptable carrier"
includes any and all solvents, dispersion media, coatings, isotonic
and absorption delaying agents, sweeteners and the like. These
pharmaceutically acceptable carriers may be prepared from a wide
range of materials including, but not limited to, diluents, binders
and adhesives, lubricants, disintegrants, coloring agents, bulking
agents, flavoring agents, sweetening agents and miscellaneous
materials such as buffers and absorbents that may be needed in
order to prepare a particular therapeutic composition. The use of
such media and agents for pharmaceutically active substances is
well known in the art. Except insofar as any conventional media or
agent is incompatible with the active ingredients, its use in
preferred compositions is contemplated. In one embodiment, talc,
and magnesium stearate are included in the formulation. Other
ingredients known to affect the manufacture of this composition as
a dietary bar or functional food can include flavorings, sugars,
amino-sugars, proteins and/or modified starches, as well as fats
and oils.
[0122] Dietary supplements, lotions or therapeutic compositions of
preferred embodiments can be formulated in any manner known by one
of skill in the art. In one embodiment, the composition is
formulated into a capsule or tablet using techniques available to
one of skill in the art. In capsule or tablet form, the recommended
daily dose for an adult human or animal would preferably be
contained in one to six capsules or tablets. However, preferred
compositions can also be formulated in other convenient forms, such
as an injectable solution or suspension, a spray solution or
suspension, a lotion, gum, lozenge, food or snack item. Food,
snack, gum or lozenge items can include any ingestible ingredient,
including sweeteners, flavorings, oils, starches, proteins, fruits
or fruit extracts, vegetables or vegetable extracts, grains, animal
fats or proteins. Thus, preferred compositions can be formulated
into cereals, snack items such as chips, bars, gumdrops, chewable
candies or slowly dissolving lozenges. Preferred embodiments
contemplate treatment of all types of inflammation-based diseases,
both acute and chronic. Preferred formulations reduce the
inflammatory response and thereby promotes healing of, or prevents
further damage to, the affected tissue. A pharmaceutically
acceptable carrier can also be used in the preferred compositions
and formulations.
Assay using AGS Cell Line
[0123] The Kuhrts patent application referenced previously attempts
to identify therapeutic components based on the Modified Whole
Blood/Cell Assay of T. D. Warner et al., Nonsteroid drug
selectivities for cyclooxygenase-1 rather than cyclooxygenase-2 are
associated with human gastrointestinal toxicity: A full in vitro
analysis, Proc. Natl. Sci. USA 96:7563-68(1999) in paragraph [46].
When tested according to this procedure, hops extracts do not yield
IC.sub.50 values in the necessary .mu.g/mL range, since they are
not direct inhibitors of COX-2. This lack of direct inhibition of
COX-2 was demonstrated by Tobe, H. et al. 1997. (Bone resorption
Inhibitors from hop extract. Biosci. Biotech. Biochem 61(1)158-159)
using purified COX-2 enzyme. Similarly, EXAMPLE 4 of this
application demonstrates that, when tested according to the
Modified Whole Blood/Cell Assay, hops compounds and derivatives
produce median inhibitory concentrations greater than 25 .mu.g/mL.
Such high median inhibitory concentrations are pharmacologically
unsuitable. Therefore, the Modified Whole Blood Assay as described
by Warner is an invalid procedure for formulating potentially
therapeutically effective combinations containing hops or hops
derivatives.
[0124] The discovery of COX-2 has made possible the design of drugs
that reduce inflammation without removing the protective PGs in the
stomach and kidney made by COX-1. One of our approaches is to
screen compositions of the preferred embodiments using in vitro
animal cells to assess COX-2 and COX-1 inhibitory activity
employing PGE.sub.2, which has cytoprotective actions and play a
role in maintaining the integrity of the gastrointestinal mucosa,
as an endpoint. Secondarily, different cell types are used to
confirm results. The screening process would indicate compositions
that have specific COX-2 activity and limited COX-1 inhibition.
Compositions of preferred embodiments can be tested in two cell
types: 1) human pulmonary cells or other cell line to determine and
identify optimal amounts and ratios for compositions comprising
more than one component; and 2) human gastric epithelial cells (AGS
cell line), a gastrointestinal tract cell line and a model system
for assessing toxicity which is typically related to inhibition of
COX-1 which is required for wound healing (such as ulcers). Hence,
compositions of preferred embodiments that can inhibit COX-2 or
COX-2 induction can be screened by selecting compositions that have
low or no activity in AGS cells and good activity in human
pulmonary cells or other cell line.
[0125] The description below is of specific examples setting forth
preferred embodiments and are not intended to limit the scope.
EXAMPLE 1
AGS Gastric Mucosal Cells Constitutively Express Both
Cyclooxygenase-1 and Cyclooxygenase-2
[0126] Summary--This example demonstrates that the AGS human
gastric mucosal cell line, possessing constitutive expression of
COX-1 and COX-2, has excellent potential to serve as a model for
assessing the gastrointestinal toxicity of
cyclooxygenase-inhibiting compounds.
[0127] Equipment used in this example included: an OHAS Model
#E01140 analytical balance, a Forma Model #F1214 biosafety cabinet
(Marietta, Ohio), various pipettes to deliver 0.1 to 100 .mu.L
(VWR, Rochester, N.Y.), a cell hand tally counter (VWR Catalog
#23609-102, Rochester, N.Y.), a Forma Model #F3210 CO.sub.2
incubator (Marietta, Ohio), a hemacytometer (Hausser Model #1492,
Horsham, Pa.), a Leica Model #DM IL inverted microscope (Wetzlar,
Germany), a PURELAB Plus Water Polishing System (U.S. Filter,
Lowell, Mass.), a 4.degree. C. refrigerator (Forma Model #F3775,
Marietta, Ohio), a vortex mixer (VWR Catalog #33994-306, Rochester,
N.Y.), and a 37.degree. C. water bath (Shel Lab Model #1203,
Cornelius, Oreg.).
[0128] Chemicals and reagents--Prostaglandin E.sub.2 EIA kit
Monoclonal was purchased from Cayman Chemical (Ann Arbor, Mich.).
Anti-COX-1 and anti-COX-2 rabbit polyclonal antisera were obtained
from Upstate Biotechnology (CITY, N.Y.); donkey anti-goat IgG-HRP
was procured from Santa Cruz Biotechnology (City, Calif.). Heat
inactivated Fetal Bovine Serum (FBS-HI Cat. #35-011CV), and
Dulbeco's Modification of Eagle's Medium (DMEM Cat #10-013CV) was
purchased from Mediatech (Herndon, Va.). All standard reagents were
obtained from Sigma (St. Louis, Mo.) and were the purest
commercially available.
[0129] Cell Culture--The human gastric mucosal cell line AGS was
obtained from the American Type Culture Collection (Manassas, Va.)
and sub-cultured according to the instructions of the supplier. The
cells were routinely cultured at 37.degree. C. with 5% CO.sub.2 in
RPMI 1640 containing 10% FBS, with 50 units penicillin/mL, 50 .mu.g
streptomycin/mL, 5% sodium pyruvate, and 5% L-glutamine.
Exponentially growing cells were seeded into 6-well plates and
grown to confluence. A 20 .mu.L aliquot of the supernatant media
was sampled for determination of PGE.sub.2 content. Cells were then
washed in PBS, scraped and lysed for immunoblotting.
[0130] Protein assay--Protein concentrations of cell lysates were
determined using the NanoOrange Protein Quantitation Kit with
bovine serum albumin as the standard (Molecular Probes, Eugene,
Oreg.) according to the procedure supplied by the manufacturer.
Fluorescence was determined using a Packard FluoroCount, Model BF
10000 fluorometer with the excitation filter set at 485 nm and
emission filter set at 570 nm using Packard PlateReader version 3.0
software. The I-Smart program provided with the Packard PlateReader
was used to calculate the protein concentration.
[0131] Immunoblotting--Western blotting of COX-1 and COX-2 was
performed using PAGErTM Gold Precast Gels (Bio Whittaker Molecular
Applications (Rockland, Me.). AGS cell lysates containing
approximately 60 .mu.g protein were loaded with Laemmli Sample
Buffer into the wells of the gel in a total volume of 30 .mu.IL.
The vertical minigel electrophoresis chambers were made by Savant
Instruments Inc. (Holbrook, N.Y.), model MV 120. Gels were run at
40 mA/plate (constant current) at room temperature until the
bromophenol blue stain reached the bottom of the gel, about one h.
Gels were then blotted on the polyvinyl fluoride transfer membranes
(Pall Corporation, Ann Arbor, Mich.), overnight, at 500 mA and
4.degree. C. Precision Protein Standard molecular weight markers,
unstained, broad range (BioRad, Hercules, Calif.) were used. The
BioWest.TM. Extended duration chemiluminescent substrate, a
non-isotopic, horseradish peroxidase substrate kit for Western blot
detection (BioImaging Systems, Upland, Calif.) was used for protein
visualization. Images of western blots were acquired using a UVP
Epi Chemi II Darkroom (BioImaging Systems), analyzed and enhanced
by LabWorks.TM. Image Acquisition and Analysis Software (BioImaging
Systems).
[0132] PGE.sub.2 assay--A commercial, non-radioactive procedure for
quantification of PGE.sub.2 was employed (Caymen Chemical, Ann
Arbor, Mich.) and the recommended procedure of the manufacturer was
used without modification. Briefly, 25 .mu.L of the medium, along
with a serial dilution of PGE.sub.2 standard samples, were mixed
with appropriate amounts of acetylcholinesterase-labeled tracer and
PGE.sub.2 antiserum, and incubated at room temperature for 18 h.
After the wells were emptied and rinsed with wash buffer, 200 .mu.L
of Ellman's reagent containing substrate for acetylcholinesterase
were added. The reaction was carried out on a slow shaker at room
temperature for 1 h and the absorbance at 415 nm was determined.
The PGE.sub.2 concentration was represented as picograms per
10.sup.5 cells.
[0133] Results--As seen in FIG. 6, the AGS cell line constitutively
expresses both COX-1 and COX-2, with COX-1 expression approximately
4-times greater than COX-2 expression. PGE.sub.2 synthesis in AGS
cells over 18 h was 660 pg/10.sup.5 cells. Thus, this example
demonstrates that the AGS human gastric mucosal cell line,
possessing constitutive expression of COX-1 and COX-2, has
excellent potential to serve as a model for assessing the
gastrointestinal toxicity of cyclooxygenase-inhibiting
compounds.
[0134] In the past, the classical COX-2 hypothesis has downplayed
the role of COX-2 expression in the gastrointestinal mucosa. While
in normal gastric mucosa COX- 1 is the predominant COX isozyme, as
demonstrated in this example and in the literature, there is
increasing evidence that detectable amount of COX-2 mRNA and
protein are both constitutively expressed and inducible in specific
locations of the gastric mucosa in both animals and humans [Halter,
F., et al. (2001) Cyclooxygenase 2-implications on maintenance of
gastric mucosal integrity and ulcer healing: controversial issues
and perspectives. Gut 49, 443-453]. Recent studies in rats have
shown that whereas selective inhibition of COX-1 or COX-2 is not
ulcerogenic, combined inhibition of both COX-1 and COX-2 induces
severe lesions in the stomach and small intestine comparable with
the effects of NSAID such as indomethacin. This observation
suggests an important contribution of COX-2 to the maintenance of
gastrointestinal mucosal integrity.
EXAMPLE 2
Inhibition of PGE.sub.2 Synthesis in Gastric Mucosal Cells by
Nonsteroidal Anti-Inflammatory Drugs
[0135] Summary--This example illustrates that inhibition of
PGE.sub.2 synthesis in AGS gastric cells by NSAIDs correlates with
their observed clinical gastric irritation.
[0136] Chemicals--Rofecoxib and celexocib were obtained.
Diisofluorophosphate (DIFP), nimensulide, ibuprofen, salicylic
acid, aspirin, indomethacin and acetaminophen were purchased from
Sigma (St. Louis, Mo.). All other chemicals were obtained from
suppliers as described in Example 1.
[0137] Cells--A549 (human pulmonary epithelial) and AGS cells
(human gastric mucosa) were obtained from the American Type Culture
Collection (Manassas, Va.) and sub-cultured according to the
instructions of the supplier. The cells were routinely cultured at
37.degree. C. with 5% CO.sub.2 in RPMI 1640 containing 10% FBS,
with 50 units penicillin/mL, 50 .mu.g streptomycin/mL, 5% sodium
pyruvate, and 5% L-glutamine. On the day of the experiments,
exponentially growing cells were harvested and washed with
serum-free RPMI 1640.
[0138] The log phase A549 and AGS cells were plated at
8.times.10.sup.4 cells per well in 0.2 mL growth medium per well in
a 96-well tissue culture plate. For the determination of PGE.sub.2
inhibition by the test compounds in A549 cells, the procedure of
Warner et al., also known as the WHMA-COX-2 protocol [Warner, T.
D., et al. (1999) Nonsteroid drug selectivities for
cyclo-oxygenase-1 rather than cyclo-oxygenase-2 are associated with
human gastrointestinal toxicity: a full in vitro analysis. Proc
Natl Acad Sci U S A 96, 7563-7568.] was followed with no
modifications. Briefly, 24 hours after plating of the A549 cells,
interleukin-1.beta. (10 ng/mL) was added to induce the expression
of COX-2. After 24 hr, the cells were washed with serum-free RPMI
1640 and the test materials, dissolved in DMSO and serum-free RPMI,
were added to the wells to achieve final concentrations of 25, 5.0,
0.5 and 0.05 .mu.g/mL. Each concentration was run in duplicate.
DMSO was added to the control wells in an equal volume to that
contained in the test wells. Sixty minutes later, A23187 (50 .mu.M)
was added to the wells to release arachidonic acid. Twenty-five
.mu.L of media were sampled from the wells 30 minutes later for
PGE.sub.2 determination.
[0139] Non-stimulated AGS cells were used in these studies.
Twenty-four hours after plating in the 96-well microtiter plates,
the cells were washed with serum-free RPMI 1640 and the test
materials, dissolved in DMSO and serum-free RPMI, were added to the
wells to achieve final concentrations of 25, 5.0, 0.5 and 0.05
.mu.g/mL. Each concentration was run in duplicate. DMSO was added
to the control wells in an equal volume to that contained in the
test wells. Sixty minutes later, arachidonic acid was added to the
wells to achieve a final concentration of 100 .mu.M. Twenty-five
.mu.L of media were sampled from the wells 30 minutes after the
addition of arachidonic acid for PGE.sub.2 determination.
[0140] Cell viability--Cell viability was assessed by a
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT)-based colorimetric assay (Sigma, St. Louis, Mo.). The MTT
solution was added directly to the wells after sampling for
PGE.sub.2 determination. The absorbance of each well was read at
580 nm using an ELISA plate reader. No toxicity was observed at the
highest concentrations tested for any of the compounds.
[0141] Calculations--The median inhibitory concentration
(IC.sub.50) for PGE.sub.2 synthesis was calculated using CalcuSyn
(BIOSOFT, Ferguson, Mo.). This statistical package performs
multiple drug dose-effect calculations using the median effect
methods described by T-C Chou and P. Talaly [(1984) Quantitative
analysis of dose-effect relationships: the combined effects of
multiple drugs or enzyme inhibitors. Adv Enzyme Regul 22, 27-55.]
hereby incorporated by reference.
[0142] Briefly, the analysis correlates the "Dose" and the "Effect"
in the simplest possible form: fa/fu=(C/C.sub.m).sup.m, where C is
the concentration or dose of the compound and Cm is the
median-effective dose signifying the potency. Cm is determined from
the x-intercept of the median-effect plot. The fraction affected by
the concentration of the test material is fa and the fraction
unaffected by the concentration is fu (fu=1-fa). The exponent m is
the parameter signifying the sigmoidicity or shape of the
dose-effect curve. It is estimated by the slope of the
median-effect plot.
[0143] The median-effect plot is a graph of x=log(C) vs
y=log(fa/fu) and is based on the logarithmic form of Chou's
median-effect equation. The goodness of fit for the data to the
median-effect equation is represented by the linear correlation
coefficient r of the median-effect plot. Usually, the experimental
data from enzyme or receptor systems have an r>0.96, from tissue
culture an r>0.90 and from animal systems an r>0.85. In the
cell-based studies reported here, all linear correlation
coefficients were greater than 0.90. Experiments were repeated
three times on three different dates. The percent inhibition at
each dose was averaged over the three independent experiments and
used to calculate the median inhibitory concentrations
reported.
[0144] Results--The highly specific COX-2 inhibitor
diisofluorophosphate exhibited a median inhibitory concentration in
A549 cells of 1.19 .mu.g/mL and did not inhibit PGE.sub.2 synthesis
in AGS cells at the highest concentration tested of 25 .mu.g/mL
(Table 3). Rofecoxib, and celexocib, selective COX-2 drugs, were
27-, and 14-times, respectively, more potent inhibitors of
PGE.sub.2 synthesis in the target A549 cells than in the non-target
AGS gastric mucosal cells. This finding demonstrates not only COX-2
selectivity, but also target-tissue selectivity consistent with
their low gastrointestinal toxicity. Nimensulide, another new,
selective COX-2 inhibitor was equally as potent in the inhibition
of PGE.sub.2 synthesis in both cell lines. The anti-inflammatory
agent acetaminophen, purported to inhibit an unidentified isozyme
of COX (COX-3) and having low gastrointestinal toxicity, inhibited
PGE.sub.2 biosynthesis in A549 cells but had no effect on PGE.sub.2
synthesis in AGS gastric mucosal cells.
[0145] Alternatively and consistent with their demonstrated
clinical gastric toxicity, ibuprofen, aspirin and indomethacin all
exhibited more inhibition of PGE.sub.2 synthesis in the AGS cell
line than in the target A549 cells. Salicylic acid, an
anti-inflammatory agent that inhibits the expression of COX-2 with
little gastric irritation, was inactive in both cell models.
TABLE-US-00003 TABLE 3 Median inhibitory concentrations for test
compounds in the A549 and AGS cell lines. IC.sub.50 A549 IC.sub.50
AGS Compound [.mu.g/mL] [.mu.g/mL] IC.sub.50 AGS/IC.sub.50 A549
Diisofluorophosphate 1.19 >25 >21 Rofecoxib 0.081 2.21 27.3
Celexocib 0.004 0.055 13.8 Nimensulide 0.10 0.11 1.0 Ibuprofen 0.10
0.05 0.50 Aspirin 0.48 0.09 0.19 Indomethacin 0.033 0.002 0.002
Salicylic acid >25 >25 >1 Acetaminophen 0.607 >25
>41
[0146] These results validate the use of the AGS gastric mucosal
cell line to evaluate potential gastrointestinal toxicity of
anti-inflammatory agents capable of inhibiting the synthesis of
PGE.sub.2. They also demonstrate cellular specificity in the action
of COX-inhibiting compounds. A ratio of 1 for IC.sub.50
AGS/IC.sub.50 A549 indicates IC.sub.50s that are the same for both
the AGS cell and A549 cells. If the ratio is higher than 1 for
IC.sub.50 AGS/IC.sub.50 A549, then the inhibition of PGE.sub.2 is
lower for the AGS cells. A lower inhibition of PGE.sub.2 in AGS
cells is favorable because AGS cell line expresses more COX-1,
which maintains mucosal homeostasis.
EXAMPLE 3
Inhibition of PGE.sub.2 Synthesis in Stimulated and Nonstimulated
Murine Macrophages by Hops (Humulus lupulus) Compounds and
Derviatives
[0147] Summary--This example illustrates the potency of hops
fractions and derivatives to inhibit COX-2 synthesis of PGE.sub.2
preferentially over COX-1 synthesis of PGE.sub.2 in the murine
macrophage model.
[0148] Chemicals and reagents--Bacterial lipopolysaccharide (LPS; B
E. coli 055:B5) was from Sigma (St. Louis, Mo.). Hops fractions (1)
alpha hop (1% alpha acids; AA), (2) aromahop OE (10% beta acids and
2% isomerized alpha acids , (3) isohop (isomerized alpha acids;
IAA), (4) beta acid solution (beta acids BA), (5) hexahop gold
(hexahydro isomerized alpha acids; HHIAA), (6) redihop (reduced
isomerized-alpha acids; RIAA), (7) tetrahop (tetrahydro-iso-alpha
acids THIAA) and (8) spent hops were obtained from Betatech Hops
Products (Washington, D.C., U.S.A.). The spent hops were extracted
two times with equal volumes of absolute ethanol. The ethanol was
removed by heating at 40.degree. C. until a only thick brown
residue remained. This residue was dissolved in DMSO for testing in
RAW 264.7 cells. Unless otherwise noted, all standard reagents were
obtained from Sigma (St. Louis, Mo.) and were the purest
commercially available. All other chemicals and equipment were as
described in Examples 1 and 2.
[0149] Cell culture--RAW 264.7 cells, obtained from American Type
Culture Collection (Catalog #TIB-71, Manassas, Va.), were grown in
Dulbecco's Modification of Eagle's Medium (DMEM, Mediatech,
Herndon, Va.) and maintained in log phase. The DMEM growth medium
was made by adding 50 mL of heat inactivated FBS and 5 mL of
penicillin/streptomycin to a 500 mL bottle of DMEM and storing at
4.degree. C. The growth medium was warmed to 37 .degree. C. in
water bath before use.
[0150] On day one of the experiment, the log phase RAW 264.7 cells
were plated at 8.times.10.sup.4 cells per well in 0.2 mL growth
medium per well in a 96-well tissue culture plate in the morning.
At the end of the day one (6 to 8 h post plating), 100 .mu.L of
growth medium from each well were removed and replaced with 100
.mu.L fresh medium.
[0151] A 1.0 mg/mL stock solution of LPS, used to induce the
expression of COX-2 in the RAW 264.7 cells, was prepared by
dissolving 1.0 mg of LPS in 1 mL DMSO. It was vortexed until
dissolved and stored at 4.degree. C. Before use, it was melted at
room temperature or in a 37.degree. C. water bath.
[0152] On day two of the experiment, test materials were prepared
as 1000.times. stock in DMSO. In 1.7 .mu.L microfuge tubes, 1 mL
DMEM without FBS was added for test concentrations of 0.05, 0.10,
0.5, and 1.0 .mu.g/mL. Two .mu.L of the 1000.times. DMSO stock of
the test material was added to the 1 mL of medium without FBS. The
tube contained the final concentration of the test material
concentrated 2-fold and the tube placed in an incubator for 10
minutes to equilibrate to 37.degree. C.
[0153] For COX-2 associated PGE.sub.2 synthesis, 100 .mu.L of
medium were removed from each well of the cell plates prepared on
day one and replaced with 100 .mu.L of equilibrated 2.times. final
concentration of the test compounds. Cells were then incubated for
90 minutes. Twenty .mu.L of LPS were added to each well of cells to
be stimulated to achieve a final concentration of 1 .mu.g LPS/mL
and the cells were incubated for 4 h. The cells were further
incubated with 5 .mu.M arachidonic acid for 15 minutes. Twenty-five
.mu.L of supernatant medium from each well was transferred to a
clean microfuge tube for the determination of PGE.sub.2 released
into the medium.
[0154] Following the LPS stimulation, the appearance of the cells
was observed and viability was determined as described in Example
2. No toxicity was observed at the highest concentrations tested
for any of the compounds. Twenty-five .mu.L of supernatant medium
from each well was transferred to a clean microfuge tube for the
determination of PGE.sub.2 released into the medium. PGE.sub.2 was
determined and reported as previously described in Example 1.
[0155] For COX-1 associated PGE.sub.2 synthesis, 100 .mu.L of
medium were removed from each well of the cell plates prepared on
day one and replaced with 100 .mu.L of equilibrated 2.times. final
concentration of the test compounds. Cells were then incubated for
90 minutes. Next, instead of LPS stimulation, the cells were
incubated with 100 .mu.M arachidonic acid for 15 minutes.
Twenty-five .mu.L of supernatant medium from each well was
transferred to a clean microfuge tube for the determination of
PGE.sub.2 released into the medium. The appearance of the cells was
observed and viability was determined as described in Example 2. No
toxicity was observed at the highest concentrations tested for any
of the compounds. Twenty-five .mu.L of supernatant medium from each
well was transferred to a clean microfuge tube for the
determination of PGE.sub.2 released into the medium. PGE.sub.2 was
determined and reported as previously described in Example 1. The
median inhibitory concentrations (IC.sub.50) for PGE.sub.2
synthesis from both COX-2 and COX-1 were calculated as described in
Example 2. TABLE-US-00004 TABLE 4 COX-2 and COX-1 inhibition in RAW
264.7 cells by hop fractions and derviatives COX-2 COX-1 IC.sub.50
IC.sub.50 Test Material [.mu.g/mL] [.mu.g/mL] COX-1/COX-2 Alpha hop
(AA) 0.21 6.2 30 Aromahop OE 1.6 4.1 2.6 Isohop (IAA) 0.13 18 144
Beta acids (BA) 0.54 29 54 Hexahop (HHIAA) 0.29 3.0 11 Redihop
(RIAA) 0.34 29 87 Tetrahop (THIAA) 0.20 4.0 21 Spent hops (EtOH)
0.88 21 24
[0156] As seen in Table 4, all hops fractions and derivative
selectively inhibited COX-2 over COX-1 in this target macrophage
model. This was a novel and unexpected finding. The extent of COX-2
selectivity for the hops derivatives IAA and RLAA, respectively,
144- and 87-fold, was unanticipated. Such high COX-2 selectivity
combined with low median inhibitory concentrations, has not been
previously reported for natural products from other sources.
EXAMPLE 4
Hops Compounds and Derivatives are not Direct Cyclooxygenase Enzyme
Inhibitors
[0157] Summary--This example illustrates that hops compounds and
derivatives do not inhibit PGE.sub.2 synthesis in A549 pulmonary
epithelial cells at physiologically relevant concentrations when
tested using the WHMA-COX-2 protocol.
[0158] Chemicals--Hops and hops derivatives used in this example
were previously described in Example 3. All other chemicals were
obtained from suppliers as described in Examples 1 and 2.
[0159] Cells--A549 (human pulmonary epithelial) Cells were obtained
from the American Type Culture Collection (Manassas, Va.) and
sub-cultured according to the instructions of the supplier. The
cells were routinely cultured at 37.degree. C. with 5% CO.sub.2 in
RPMI 1640 containing 10% FBS, with 50 units penicillin/mL, 50 .mu.g
streptomycin/mL, 5% sodium pyruvate, and 5% L-glutamine. On the day
of the experiments, exponentially growing cells were harvested and
washed with serum-free RPMI 1640.
[0160] Log phase A549 cells were plated at 8.times.10.sup.4 cells
per well with 0.2 mL growth medium per well in a 96-well tissue
culture plate. For the determination of PGE.sub.2 inhibition by the
test compounds, the procedure of Warner et al. [(1999) Nonsteroid
drug selectivities for cyclo-oxygenase-1 rather than
cyclo-oxygenase-2 are associated with human gastrointestinal
toxicity: a full in vitro analysis. Proc Natl Acad Sci U S A 96,
7563-7568], also known as the WHMA-COX-2 protocol was followed with
no modification. Briefly, 24 hours after plating of the A549 cells,
interleukin-1.beta. (10 ng/mL) was added to induce the expression
of COX-2. After 24 hr, the cells were washed with serum-free RPMI
1640 and the test materials, dissolved in DMSO and serum-free RPMI,
were added to the wells to achieve final concentrations of 25, 5.0,
0.5 and 0.05 .mu.g/mL. Each concentration was run in duplicate.
DMSO was added to the control wells in an equal volume to that
contained in the test wells. Sixty minutes later, A23187 (50 .mu.M)
was added to the wells to release arachidonic acid. Twenty-five
.mu.L of media were sampled from the wells 30 minutes later for
PGE.sub.2 determination.
[0161] Cell viability was assessed as previously described in
Example 2. No toxicity was observed at the highest concentrations
tested for any of the compounds. PGE.sub.2 in the supernatant
medium was determined and reported as previously described in
Example 1.
[0162] The median inhibitory concentration (IC.sub.50) for
PGE.sub.2 synthesis was calculated as previously described in
Example 2.
[0163] Results--At the doses tested, the experimental protocol
failed to capture a median effective concentration of any of the
hops extracts or derivatives. Since the protocol requires the
stimulation of COX-2 expression prior to the addition of the test
compounds, the likely answer to the failure of the test materials
to inhibit PGE.sub.2 synthesis is that their mechanism of action is
to inhibit the expression of the COX-2 isozyme and not activity
directly. While some direct inhibition can be observed using the
WHMA-COX-2 protocol, this procedure is inappropriate in evaluating
the anti-inflammatory properties of hops compounds or derivatives
of hops compounds.
EXAMPLE 5
Lack of Inhibition of PGE.sub.2 Synthesis in Gastric Mucosal Cells
by Hops (Humulus lupulus) Compounds and Derviatives
[0164] Summary--This example illustrates the lack of PGE.sub.2
inhibition by hops fractions and in the AGS human gastric mucosal
cell line implying low gastric irritancy potential of these
compounds.
[0165] Chemicals and reagents were used as described in Example 3.
AGS cells were grown and used for testing hops compounds and
derivatives as described in Example 2. PGE.sub.2 was determined and
reported as previously described in Example 1. The median
inhibitory concentrations (IC.sub.50) for PGE.sub.2 synthesis from
AGS cells were calculated as described in Example 2. TABLE-US-00005
TABLE 5 Lack of PGE.sub.2 inhibition in AGS gastric mucosal cells
by hop fractions and derviatives IC.sub.50 AGS Test Material
[.mu.g/mL] Alpha hop (AA) >25 Aromahop OE >25 Isohop (IAA)
>25 Beta acids (BA) >25 Hexahop (HHIAA) >25 Redihop (RIAA)
>25 Tetrahop (THIAA) >25 Spent hops (EtOH) >25
[0166] As seen in Table 5, all hops fractions and derivatives were
unable to inhibit PGE.sub.2 synthesis by 50% or more at the highest
concentrations tested in the AGS gastric mucosal cell line. Based
on the anti-inflammatory potency exhibited by these fractions in
target macrophages, this was a novel and unexpected finding.
EXAMPLE 6
Inhibition of PGE.sub.2 Synthesis by Rosemary Extract and Compounds
Found in Rosemary
[0167] Summary--This example illustrates the anti-inflammatory
effect of rosemary extract and compounds commonly found in
rosemary, carnosic acid, ursolic acid and oleanolic acid in target
cells and the effect of rosemary extract and oleanolic acid on
PGE.sub.2 synthesis in gastrointestinal cells.
[0168] Equipment used, chemicals, cell handing and calculation of
median inhibitory concentrations were performed as previously
described in Examples 1, 2 and 3. Carnosic acid, ursolic acid and
oleanolic acid were obtained from Sigma (St. Louis, Mo.). The
rosemary extract was a hexane extract obtained from selected leaves
of Rosmarinus officinalis by mean (95% +/-3% rosemary extract) that
complied with US regulation (21 CFR 101-22). It was determined by
HPLC analysis that the extract contained a minimum of 11% phenolic
diterpenes (consisting of carnosic acid, carnosol, methyl
carnosate, rosemadial, rosemarinic acid), 4.9% min carnosic acid,
and a minimum of 7.6% the sum of carnosol+carnosic acid. The
carnosic acid was purchased from Sigma (St. Louis, Mo.) and the
oleanolic acid (80%) was obtained from Sabinsa (121 Ethel Road
West, Piscataway, N.J.). TABLE-US-00006 TABLE 6 PGE.sub.2
inhibition in RAW 264.7 and AGS cells by a rosemary extract,
carnosic acid, ursolic acid, and oleanolic acid. RAW RAW 264.7 or
AGS IC.sub.50 IC.sub.50 Test Material (COX-2/COX-1).dagger.
[.mu.g/mL] [.mu.g/mL] COX-1/COX-2 Rosemary extract (RAW/AGS) 0.51
4.0 7.8 Carnosic acid (RAW/RAW) 0.50 231 470 Ursolic acid (RAW/RAW)
1.91 33 17 Oleanolic acid (RAW/RAW) 1.15 19 17 Oleanolic acid
(RAW/AGS) 1.15 5.0 4.3 .dagger.Indicates the cell lines used to
estimate inhibitor effects, respectively, on COX-2 or COX-1
synthesis of PGE.sub.2. In all cases, LPS-stimulated RAW 264.7
cells were used to determine median inhibitory concentrations of
COX-2 mediated PGE.sub.2 synthesis. For the estimation of the
effects of test materials on COX-1-mediated synthesis, either
non-stimulated RAW264.7 or non-stimulated AGS cells were used.
[0169] Results--All test materials exhibited potent inhibition of
PGE.sub.2 synthesis in LPS-stimulated RAW 264.7 cells indicating
inhibition of the COX-2 isozyme (Table 6). Surprisingly, the
rosemary extract was more potent than ursolic and oleanolic acids
and equal to pure carnosic acid in potency with a median inhibitory
concentration of 0.5 .mu.g test material/mL medium. Since the
rosemary extract contained only 11% carnosic acid or derivative,
the inference is that the interaction of the carnosic acid
derivatives or the myriad of other compounds in the rosemary
extract were acting in concert or synergistically to provide such a
potent inhibition of COX-2. Alternatively, one of the compounds
previously identified in rosemary and listed earlier has extremely
high potency for inhibiting COX-2 mediated synthesis of
PGE.sub.2.
[0170] In non-stimulated RAW 264.7 cells, the pure compounds were
relatively inactive exhibiting IC.sub.50 values of 231, 33 and 19
.mu.g/mL, respectively, for carnosic, ursolic and oleanolic acids.
This indicated a strong preference for COX-2 inhibition over COX-1
for synthesis of PGE.sub.2 in the RAW 264.7 target cell model. This
extent of COX isozyme selectivity has never been reported in the
literature and was an unexpected result. In the AGS gastric mucosal
cell line, however, both the rosemary extract and oleanolic acid
exhibited potent inhibition of PGE.sub.2 synthesis.
EXAMPLE 7
Synergistic Inhibition of PGE.sub.2 Synthesis Target Cells by Hops
CO.sub.2-Extract in Combination with Triterpenoids Oleanolic Acid
and Ursolic Acid
[0171] Equipment used, chemicals, cell handing and calculation of
median inhibitory concentrations were performed as previously
described in Examples 1, 2 and 3. The hops CO2-extract was
purchased from Hopunion, (Yakama, Wash.) and contained 30 to 60%
alpha-acids and 15 to 45% beta-acids. Oleanolic and ursolic acids
and were obtained from Sigma (St. Louis, Mo.) and were the highest
purity commercially available (>98%).
[0172] Synergy of test components was quantified using the
combination index (CI) parameter. The CI of Chou-Talaly is based on
the multiple drug-effect and is derived from enzyme kinetic models
(Chou, T. -C. and Talalay, P. (1977) A simple generalized equation
for the analysis of multiple inhibitions of Michaelis-Menten
kinetic systems. J. Biol. Chem. 252:6438-6442). The equation
determines only the additive effect rather than synergism or
antagonism. However, we define synergism as a more than expected
additive effect, and antagonism as a less than expected additive
effect as proposed by Cho and Talalay Using the designation of CI=1
as the additive effect, we obtain for mutually exclusive compounds
that have the same mode of action or for mutually non-exclusive
drugs that have totally independent modes of action the following
relationships: CI<1,=1, and >1 indicating synergism,
additivity and antagonism, respectively.
[0173] Results--The 4:1 (CO.sub.2-extract:triterpenoid) combination
tested in RAW 264.7 cells exhibited potent synergy over the entire
dose-response curve. Combination indexes computed for both test
materials at the IC.sub.50, IC.sub.75 and IC.sub.90 are presented
in Table 7. As described in this example, the synergy of these
combinations covered a concentration range of 0.001 to 50 .mu.g/mL
of each component of the combination. TABLE-US-00007 TABLE 7
Computed Combination Indexes for the dose-response curves of 1:4
combinations of a CO.sub.2-extract of hops and the triterpenes
oleanolic and ursolic acid Test Material CI.sub.50 CI.sub.75
CI.sub.90 Mean CI CO.sub.2-Extract:Oleanolic acid [1:4] 0.514 0.461
0.414 0.463 CO.sub.2-Extract:Ursolic acid [1:4] 0.529 0.650 0.806
0.662
EXAMPLE 8
Synergistic Inhibition of PGE.sub.2 Synthesis by Hops Combinations
with an Extract of Rosemary in Target and Nontarget Cells
[0174] Summary--This example illustrates synergy of combinations of
reduced isomerized alpha acids and rosemary extract on target A549
cells and synergistic antagonism of rosemary inhibition of
PGE.sub.2 synthesis in AGS gastric mucosal cells.
[0175] Equipment used, chemicals, cell handing and calculation of
median inhibitory concentrations were performed as previously
described in Examples 1, 2, 3 and 4. Several differences in the
protocol for testing in the A549 cells were incorporated in this
example. First, test materials were added to the medium 60 minutes
prior to stimulation with IL-1.beta.. Second, in the determination
of dose-response curves, 5 .mu.M arachidonic acid was used in place
of the calcium ionophore A23187. Synergy of the combinations was
computed as described in Example 7.
[0176] Results--Table 8 shows PGE.sub.2 inhibition by reduced
isomerized alpha-acids, rosemary extract and a 2:1 combinations of
reduced isomerized alpha-acids and rosemary extract in IL-1.beta.
stimulated A549 cells. This cell line represents a model target
cell for anti-inflammatory efficacy. Median inhibitory
concentrations for reduced isomerized alpha-acids and rosemary
extract independently were, respectively, 0.84 and 1.3 .mu.g/mL.
The 2:1 combination of reduced isomerized alpha-acids and rosemary
extract exhibited synergy at and below the median inhibitory
concentration of the combination.
[0177] Table 9 shows inhibition of PGE.sub.2 synthesis in the human
gastric AGS cells. These cells represent a model for
gastrointestinal toxicity of prostaglandin inhibitors. Test
materials exhibiting inhibition of PGE.sub.2 synthesis in these
cells would be expected to demonstrate gastric irritation and
ulceration with chronic use. The inhibition of PGE.sub.2 synthesis
by rosemary extract was synergistically antagonized by a 2:1
combination of reduced isomerized alpha-acids and rosemary extract.
This unexpected result represents a novel finding of synergistic
antagonism. TABLE-US-00008 TABLE 8 Median inhibitory concentrations
and combination index for PGE.sub.2 inhibition by reduced
isomerized alpha-acids, rosemary extract and a combination of
isomerized alpha-acids and rosemary extract in
IL-1.beta.-stimulated A549 cells Combination IC.sub.50 Index
<1.0.dagger. Test Material [.mu.g/ml] [.mu.g/mL] Reduced
isomerized alpha-acids (RIAA) 0.84 Rosemary extract 1.3
RIAA:Rosemary 2:1 0.48 At 0.48 and below .dagger.The combination
index was less than 1 over the portion of the dose-response curve
at and below the IC50 value indicating synergistic inhibition of
PGE.sub.2 synthesis by the combination at these concentrations.
[0178] TABLE-US-00009 TABLE 9 Synergy of a 1:1 combination of
reduced isomerized alpha-acids with rosemary extract resulting in a
reduction of PGE.sub.2 inhibition in AGS gastric mucosal cells.
IC.sub.50 Test Material [.mu.g/ml] Combination Index Reduced
isomerized alpha-acids (RIAA) >25 -- Rosemary 4.0 --
RIAA:Rosemary 1:1 >25 >1.0.dagger. .dagger.The combination
index was greater than 1 over the entire dose-response curve
indicating synergistic antagonism of PGE.sub.2 inhibition by the
combination.
[0179] While this example only presents the combination of rosemary
extract with one of the hops derivative, reduced isomerized
alpha-acids, it would be obvious for one skilled in the art to
assume to expect the same results with other hops derivatives that
also show no PGE.sub.2 inhibition with AGS cells at dose as high as
25 .mu.g/mL. Examples of these hops derivatives would include
isomerized-alpha acids, hexahydro-isomerized alpha acids,
tetrahydro-iso-alpha acids and extracts of spent hops.
EXAMPLE 9
Synergistic Inhibition of PGE.sub.2 Synthesis by Reduced Isomerized
Alpha-Acids and Oleanolic Acid in Target Cells with no Effect on
PGE.sub.2 Synthesis in Nontarget Cells
[0180] Summary--This example illustrates that reduced isomerized
alpha-acids exhibit strong synergy with the triterpene oleanolic
acid in the inhibition of PGE.sub.2 synthesis is the target A549
cells and synergistically antagonize oleanolic acid inhibition of
PGE.sub.2 synthesis in gastric cells.
[0181] Equipment used, chemicals, cell handing and calculation of
median inhibitory concentrations were performed as previously
described in Examples 1, 2, 3 and 4. Several differences in the
protocol for testing in the A549 cells were incorporated in this
example. First, test materials were added to the medium 60 minutes
prior to stimulation with IL-1.beta.. Second, in the determination
of dose-response curves, A549 cells remained in the presence of
test material overnight before the sampling of media for PGE.sub.2
determination. Synergy of the combinations was computed as
described in Example 7. Reduced isomerized alpha-acids were
obtained as a one percent aqueous solution from John Haas, Inc.
(Yakima, Wash.) and oleanolic acid was obtained from Sabinsa
(Piscataway, N.J.) and was 80% pure. Synergy of the combinations
was computed as described in Example 7.
[0182] Results--Table 10 shows PGE.sub.2 inhibition by oleanolic
acid, reduced isomerized alpha-acids and various combinations of
reduced isomerized alpha-acids and oleanolic acid in A549 cells.
This cell line represents a model target cell for anti-inflammatory
efficacy. Median inhibitory concentrations for reduced isomerized
alpha-acids and oleanolic acid independently were, respectively,
0.03 and 0.39 .mu.g/mL. Combinations of reduced isomerized
alpha-acids and oleanolic acid consisting of 10:1, 5:1, and 1:5,
respectively, exhibited synergy on the dose-response curve at
combined concentrations of 0.11, 0.38 and 0.76 .mu.g/mL. Thus, when
the sum of the two components was equal to or less than 0.11, 0.38
or 0.76 .mu.g/mL, their ability to inhibit PGE.sub.2 synthesis was
greater than the sum of their individual activities. TABLE-US-00010
TABLE 10 Median inhibitory concentrations and combination indexes
for PGE.sub.2 inhibition by reduced isomerized alpha-acids,
oleanolic acid and four combinations of isomerized alpha-acids and
oleanolic acid in IL-1.beta.-stimulated A549 cells. IC.sub.50 Test
Material [.mu.g/mL] Combination Index <1.0 Oleanolic acid (80%
Sabinsa) 0.390 -- Reduced isomerized alpha-acids 0.028 -- (RIAA)
RIAA:Oleanolic acid - [10:1] 0.042 At 0.11 .mu.g/mL and below
RIAA:Oleanolic acid - [5:1] 0.059 At 0.38 .mu.g/mL and below
RIAA:Oleanolic acid - [1:5] 0.022 At 0.76 .mu.g/mL and below
RIAA:Oleanolic acid - [1:10] 0.166 No .dagger.The combination index
was less than 1 over the portion of the dose-response curve at the
tabulated values indicating synergistic inhibition of PGE.sub.2
synthesis by the combination at and below these concentrations.
[0183] Table 11 shows inhibition of PGE.sub.2 synthesis in the
human gastric AGS cells. These cells represent a model for
gastrointestinal toxicity of prostaglandin inhibitors. Test
materials exhibiting inhibition of PGE.sub.2 synthesis in these
cells would be expected to demonstrate gastric irritation and
ulceration with chronic use. The inhibition of PGE.sub.2 synthesis
by oleanolic acid was synergistically antagonized by all
combinations with reduced isomerized alpha-acids. This unexpected
result represents a novel finding of synergistic antagonism.
TABLE-US-00011 TABLE 11 Synergy of reduced isomerized alpha-acids
with oleanolic acid resulting in a reduction of PGE.sub.2
inhibition in AGS gastric mucosal cells IC.sub.50 Combination Test
Material [.mu.g/mL] Index >1.0.dagger. Oleanolic acid 5.0 --
Reduced isomerized alpha-acids (RIAA >25 -- RIAA:Oleanolic acid
- [10:1] >25 Antagonism RIAA:Oleanolic acid - [5:1] >25
Antagonism RIAA:Oleanolic acid - [1:5] >25 Antagonism
RIAA:Oleanolic acid - [1:10] >25 Antagonism .dagger.When
CI>1.0 at the IC.sub.50, the combination is said to exhibit
antagonism in the inhibition of PGE.sub.2 synthesis by AGS
cells.
[0184] While this example only presents the combination of
oleanolic acid with one of the hops derivative, reduced isomerized
alpha-acids, it would be obvious for one skilled in the art to
assume to expect the same results with other hops derivatives that
also show no PGE.sub.2 inhibition with AGS cells at dose as high as
25 .mu.g/mL. Examples of these hops derivatives would include
isomerized-alpha acids, hexahydro-isomerized alpha acids,
tetrahydro-iso-alpha acids and extracts of spent hops.
EXAMPLE 10
Synergistic Inhibition of PGE.sub.2 Synthesis by a Combination of
Reduced Isomerized Alpha Acids with Tryptanthrin in Target Cells
with no Effect on PGE.sub.2 Synthesis in Nontarget Cells
[0185] Summary--This example illustrates a potent synergy of a 1:1
combination of reduced isomerized alpha acids and tryptanthrin on
target A549 cells and synergistic antagonism of tryptanthrin
inhibition of PGE.sub.2 synthesis in AGS gastric mucosal cells.
[0186] Equipment used, chemicals, cell handing and calculation of
median inhibitory concentrations were performed as previously
described in Examples 1, 2, 3, 4 and 9. Reduced isomerized
alpha-acids were obtained as a one percent aqueous solution from
John Haas, Inc. (Yakima, Wash.) and tryptanthrin was obtained from
Waco Chemicals (Richmond, Va.) and was the highest purity
commercially available. Several differences in the protocol for
testing in the A549 cells were incorporated in this example. First,
test materials were added to the medium 60 minutes prior to
stimulation with IL-1.beta.. Second, in the determination of
dose-response curves, A549 cells remained in the presence of test
material overnight before the sampling of media for PGE.sub.2
determination. Synergy of the combinations was computed as
described in Example 7.
[0187] Results--Table 12 shows PGE.sub.2 inhibition by reduced
isomerized alpha-acids, tryptanthrin and a 1:1 combination of
reduced isomerized alpha-acids and tryptanthrin in IL-1.beta.
stimulated A549 cells. This cell line represents a model target
cell for anti-inflammatory efficacy. Median inhibitory
concentrations for reduced isomerized alpha-acids and tryptanthrin
independently were, respectively, 0.0.028 and 0.30 .mu.g/mL. The
1:1 combination of reduced isomerized alpha-acids and tryptanthrin
exhibited synergy over the entire dose-response curve.
[0188] Table 13 shows inhibition of PGE.sub.2 synthesis in the
human gastric AGS cells. These cells represent a model for
gastrointestinal toxicity of prostaglandin inhibitors. Test
materials exhibiting inhibition of PGE.sub.2 synthesis in these
cells would be expected to demonstrate gastric irritation and
ulceration with chronic use. The inhibition of PGE.sub.2 synthesis
by tryptanthin was synergistically antagonized by a 1:1 combination
of reduced isomerized alpha-acids and tryptanthrin or conjugates
thereof. This unexpected result represents a novel finding of
synergistic antagonism. TABLE-US-00012 TABLE 12 Median inhibitory
concentrations and combination index for PGE.sub.2 inhibition by
reduced isomerized alpha-acids, tryptanthrin and a combination of
isomerized alpha-acids and tryptanthrin in IL-1.beta.-stimulated
A549 cells IC.sub.50 Combination Test Material [.mu.g/mL] Index
Reduced isomerized alpha-acids RIAA 0.028 -- Tryptanthrin 0.300 --
RIAA:Tryptanthrin - [1:1] 3.1 .times. 10.sup.-7 <1.0.dagger.
.dagger.The combination index was less than 1 over the entire
dose-response curve indicating synergistic inhibition of PGE.sub.2
synthesis by the combination.
[0189] TABLE-US-00013 TABLE 13 Synergy of combinations of reduced
isomerized alpha-acids with tryptanthrin resulting in a reduction
of PGE.sub.2 inhibition in AGS gastric mucosal cells. IC.sub.50
Combination Test Material [.mu.g/mL] Index Reduced isomerized
alpha-acids (RIAA) >25 -- Tryptanthrin 4.2 -- RIAA:Tryptanthrin
- [1:1] >25 >1.0.dagger. .dagger.The combination index was
greater than 1 over the entire dose-response curve indicating
synergistic antagonism of PGE.sub.2 inhibition by the
combination.
[0190] While this example only presents the combination of
trypanthrin with one of the hops derivative, reduced isomerized
alpha-acids, it would be obvious for one skilled in the art to
assume to expect the same results with other hops derivatives that
also show no PGE.sub.2 inhibition with AGS cells at dose as high as
25 .mu.g/mL. Examples of these hops derivatives would include
isomerized-alpha acids, hexahydro-isomerized alpha acids,
tetrahydro-iso-alpha acids and extracts of spent hops.
EXAMPLE 11
Ex Vivo Inhibition of PGE.sub.2 Synthesis by a Plasma Sample from a
Human Receiving a Combination Containing Hops Derivatives a
Rosemary Extract and Oleanolic Acid
[0191] Summary--This example demonstrates the presence of PGE.sub.2
inhibiting materials in a human subject following ingestion of a
5:5:1 combination of reduced isomerized alpha acids, rosemary
extract and oleanolic acid three times per day for five days.
[0192] Equipment used, chemicals, RAW 264.7 cell handing and
calculation of PGE.sub.2 concentrations were performed as
previously described in Examples 1, 2, and 3. Reduced isomerized
alpha acids, rosemary extract and oleanolic acid were as described
in Examples 3, 6 and 7, respectively. Gel caps were made to contain
200 mg reduced isomerized alpha acids, 200 mg rosemary and 40 mg
oleanolic acid in an oil base. Plasma samples were obtained from a
human volunteer prior to and five days after consuming three
capsules per day for five days. Capsules were taken at
approximately eight-hour intervals throughout the day. On the fifth
day, blood was drawn one hour before taking the last capsule and 1,
2, 4 and 7 hours after dosing. All PGE.sub.2 assays in plasma
samples were replicated eight times. Outliers were defined and
eliminated if the value was more than three standard deviations
from the group mean computed without the perceived outlier. Raw
data with and without the outliers were graphed. Concentrations of
test material in plasma relating to percent PGE.sub.2 inhibition
were estimated using a standard curve of the combination in
commercial plasma (Gibco, Grand Island, N.Y.).
[0193] FIG. 7[A] illustrates the inhibition of PGE.sub.2 synthesis
by the plasma samples at the indicated times. A 9- to 3-fold
increase in PGE.sub.2 inhibition was observed during the first
post-dosing hour. Effective half-life (time to reduce the ability
to inhibit PGE.sub.2 synthesis by one-half) of the test material
was approximately four hours.
[0194] Estimates of test material relating to the observed
percentage inhibition of PGE.sub.2 synthesis in RAW 264.7 cells are
presented in FIG. 7[B]. Using only the data with outliers removed,
a 12.5-fold increase in test material concentration was noted
during the first hour. A maximal concentration of 880 ng/mL plasma
was seen at both the 1 and 2 post-dosing hours. The concentration
half-live was approximately 2.2 hours. The lack of consistency
between the effective half-life and concentration half-life may be
inferred to be due to the synergy of components in the formulation.
Efficacy is extended due to positive and synergistic interactions
among the isomerized alpha acids, the myriad of compounds in the
rosemary extract and oleanolic acid as has been demonstrated by the
examples in this application.
EXAMPLE 12
Normalization of Joint Function Following Trauma
[0195] A representative composition of the preferred embodiments as
a dietary supplement would be in an oral formulation, i.e. tablets
or gel caps that would supply one of the following combinations:
0.1 to 10 mg isocohumulone/kg per day; 0.01 to 10 mg
dihydroadhumulone/kg per day; 0.01 to 10 mg
tetrahydro-isocohumulone/kg per day; 0.01 to 10 mg/kg per day of
hexahydro-isohumulone/kg per day for a 70 kg person.
[0196] Normalization of joint movement following physical trauma
due to exercise or repetitive movement stress would be expected to
occur following two to ten doses. This result would be expected in
all animals.
EXAMPLE 13
Normalization of Joint Function Following Trauma
[0197] A representative composition of the preferred embodiments as
a dietary supplement would be in an oral formulation, i.e. tablets
or gel caps that would supply one of the following
combinations:
[0198] 17 mg reduced isomerized alpha-acid/kg per day, 17 mg
rosemary extract/kg per day and 17 mg ursolic acid/kg per day;
[0199] 17 mg reduced isomerized alpha-acid/kg per day, 17 mg
rosemary extract/kg per day and 3.4 mg ursolic acid/kg per day;
[0200] 34 mg reduced isomerized alpha-acid/kg per day, 34 mg
rosemary extract/kg per day and 3.4 mg ursolic acid/kg per day;
[0201] 340 mg reduced isomerized alpha-acid/kg per day, 340 mg
rosemary extract/kg per day and 3.4 mg ursolic acid/kg per day;
[0202] 17 mg reduced isomerized alpha-acid/kg per day, 17 mg
rosemary extract/kg per day and 85 mg ursolic acid/kg per day;
[0203] 17 mg reduced isomerized alpha-acid/kg per day, 17 mg
rosemary extract/kg per day and 170 mg ursolic acid/kg per day;
or
[0204] 17 mg reduced isomerized alpha-acid/kg per day, 17 mg
rosemary extract/kg per day and 1700 mg ursolic acid/kg per day for
a 70 kg person.
[0205] Normalization of joint movement following physical trauma
due to exercise or repetitive movement stress would be expected to
occur following two to ten doses. This result would be expected in
all animals.
EXAMPLE 14
Clinical Effectiveness of Lotion Formulations in the Treatment of
Acne Rosacea
[0206] A lotion designed to contain one of the following:
[0207] 1. 0.1% wt of the alpha-acid humulone;
[0208] 2. 0.1% wt of the isomerized alpha-acid isocohumulone;
[0209] 3. 0.1% wt of the reduced isomerized alpha-acid
dihydro-adhumulone;
[0210] 4. 0.1% wt of the tetrahydroisoalpha-acid
tetrahydro-isocohumulone; or
[0211] 5. 0.1% wt of the hexahydroisoalpha-acid
hexahydro-isohumulone
is applied to affected areas of patients who have exhibited acne
rosacea as diagnosed by their health practitioner and confirmed by
an independent board-certified dermatologist.
[0212] Self-evaluation tests and are administered one week prior to
the study to quantify the surface area affected and redness. In
addition, similar variables are scored by the professional clinical
staff not aware of the patients treatment status. These evaluations
are repeated on Days 0, 7, 14 and 21.
[0213] Patients are randomly assigned to the test formulation or
placebo at the start of the study. The test formulation and placebo
are applied to the affected area one or two times per day.
Treatment for health conditions such as diabetes, hypertension,
etc. is allowed during the study. Scores are statistically compared
between the test formulation and the placebo for each of the four
observational periods. Patients treated with the composition of the
preferred embodiments in a lotion formulation are considered
improved if the patients' scores improve by greater than 20% from
the pre-test scores within each category evaluated. The percentage
of persons exhibiting improvement is compared between the
combination formulations and the placebo control. The difference
between the two groups is considered statistically significant if
the probability of rejecting the null hypothesis when true is less
than five percent.
EXAMPLE 15
Clinical Effectiveness of Lotion Formulations in the Treatment of
Acne Rosacea
[0214] A lotion designed to contain one of the following:
[0215] 1. 0.1% wt of the alpha-acid humulone and 0.1%
trypanthrin;
[0216] 2. 0.1% wt of the isomerized alpha-acid isocohumulone and
0.1% trypanthrin;
[0217] 3. 0.1% wt of the reduced isomerized alpha-acid
dihydro-adhumulone and 0.1% tryptanthrin;
[0218] 4. 0.1% wt of the tetrahydroisoalpha-acid
tetrahydro-isocohumulone and 0.1% tryptanthrin; or 5. 0.1% wt of
the hexahydroisoalpha-acid hexahydro-isohumulone and 0.1%
tryptanthrin
is applied to affected areas of patients who have exhibited acne
rosacea as diagnosed by their health practitioner and confirmed by
an independent board-certified dermatologist.
[0219] Self-evaluation tests and are administered one week prior to
the study to quantify the surface area affected and redness. In
addition, similar variables are scored by the professional clinical
staff not aware of the patients treatment status. These evaluations
are repeated on Days 0, 7, 14 and 21.
[0220] Patients are randomly assigned to the test formulation or
placebo at the start of the study. The test formulation and placebo
are applied to the affected area one or two times per day.
Treatment for health conditions such as diabetes, hypertension,
etc. is allowed during the study. Scores are statistically compared
between the test formulation and the placebo for each of the four
observational periods. Patients treated with the composition of the
preferred embodiments in a lotion formulation are considered
improved if the patients' scores improve by greater than 20% from
the pre-test scores within each category evaluated. The percentage
of persons exhibiting improvement is compared between the
combination formulations and the placebo control. The difference
between the two groups is considered statistically significant if
the probability of rejecting the null hypothesis when true is less
than five percent.
EXAMPLE 16
Clinical Effectiveness of a Lotion Formulation in the Treatment of
Psoriasis
[0221] This example is performed in the same manner as described in
Examples 14 and 15, except that the composition is applied to
affected areas of patients who have exhibited psoriasis as
diagnosed by their own practitioner and confirmed by an independent
board-certified dermatologist. Self-evaluation tests are
administered one week prior to the study to quantify the surface
area affected and skin condition. In addition, similar variables
are scored by the professional clinical staff not aware of the
patients treatment status. These evaluations are repeated on Days
0, 7, 30 and 60.
[0222] Patients are randomly assigned to the test formulation or
placebo at the start of the study. The test formulation and placebo
are applied to the affected area one or two times per day.
Treatment for health conditions such as diabetes, hypertension,
etc. is allowed during the study. Scores are statistically compared
between the test formulation and the placebo for each of the four
observational periods. Patients treated with the composition of the
preferred embodiments as the test lotion formulation are considered
improved if the patients' scores improve by greater than 20% from
the pre-test scores within each category evaluated. The percentage
of persons exhibiting improvement is compared between the test
formulation and the placebo control. The difference between the two
groups is considered statistically significant if the probability
of rejecting the null hypothesis when true is less than five
percent.
EXAMPLE 17
Clinical Effectiveness of a Formulation in the Treatment of
Alzheimer's Disease
[0223] An oral formulation as described in Examples 12 and 13 is
administered to patients who have manifested an early stage of
Alzheimer's Disease (AD), as diagnosed by their practitioner and
confirmed by an independent board-certified neurologist. Two weeks
before the clinical trial, the patients undergo appropriate
psychoneurological tests such as the Mini Mental Status Exam
(MMSE), the Alzheimer Disease Assessment Scale (ADAS), the Boston
Naming Test (BNT), and the Token Test (TT). Neuropsychological
tests are repeated on Day 0, 6 weeks and 3 months of the clinical
trial. The tests are performed by neuropsychologists who are not
aware of the patient's treatment regimen.
[0224] Patients are randomly assigned to the test formulation or
placebo at the start of the study. The test formulation and placebo
are taken orally one or two times per day. Treatment for conditions
such as diabetes, hypertension, etc. is allowed during the study.
Scores are statistically compared between the test formulation and
the placebo for each of the three observational periods. Without
treatment, the natural course of AD is significant deterioration in
the test scores during the course of the clinical trial. Patients
treated with the composition of the preferred embodiments as the
test formulation are considered improved if the patients' scores
remain the same or improve during the course of the clinical
trial.
EXAMPLE 18
Oral Formulation in the Treatment and Prevention of Colon
Cancer
[0225] An oral formulation as described in Examples 12 and 13 is
administered to patients who have manifested an early stage of
colon cancer as diagnosed by their own practitioner and confirmed
by a independent board-certified oncologist.
[0226] Patients are randomly assigned to the test formulation or a
placebo at the start of the study. The test formulation and placebo
are taken orally one or two times per day. Treatment for conditions
such as diabetes, hypertension, etc. is allowed during the study.
Endoscopic evaluations are made at one, two, six and twelve months.
Evidence of reappearance of the tumor during any one of the four
follow-up clinical visits is considered a treatment failure. The
percentage of treatment failures is compared between the test
formulation and the placebo control. Under the experimental
conditions described, the test material is expected to decrease the
tumor incidence with respect to the control group. The difference
between the two groups is considered statistically significant if
the probability of rejecting the null hypothesis when true is less
than five percent.
EXAMPLE 19
Oral Formulation for the Treatment of Irritable Bowel Syndrome
[0227] An oral formulation as described in Examples 12 and 13 is
administered to patients who have manifested irritable bowel
syndrome as diagnosed by their practitioner. Normal bowel
functioning is restored within 48 hours.
EXAMPLE 20
Normalization of Joint Functioning in Osteoarthritis
[0228] Using compositions described in Examples 12 and 13
normalization of joint stiffness due to osteoarthritis occurs
following five to twenty doses, in the presence or absence of
glucosamine or chondroitin sulfate. In addition, the composition
does not interfere with the normal joint rebuilding effects of
these two proteoglycan constituents, unlike traditional
non-steroidal anti-inflammatory agents.
EXAMPLE 21
Mite Dust Allergens Activate PGE.sub.2 Biosynthesis in A549
Pulmonary Cells
[0229] Summary--This example illustrates that house mite dust
allergens can induce PGE.sub.2 biosynthesis in pulmonary epithelial
cells.
Background
[0230] Sensitivity to allergens is a problem for an increasing
number of consumers. This issue has been complicated by a
surprising increase in asthma over the past few years. Asthma
suffers are especially sensitive to airborne allergens. Allergy
rates are also on the rise. This gives rise to increased awareness
of the causes of allergy symptoms and how to decrease the
associated discomfort. Approximately 10% of the population become
hypersensitized (allergic) upon exposure to antigens from a variety
of environmental sources. Those antigens that induce immediate
and/or delayed types of hypersensitivity are known as allergens.
These include products of grasses, trees, weeds, animal dander,
insects, food, drugs, and chemicals. Genetic predisposition of an
individual is believed to play a role in the development of
immediate allergic responses such as atopy and anaphylaxis whose
symptoms include hay fever, asthma, and hives.
[0231] Many allergens are protein-based molecules, and these
protein allergens can originate from many sources. It has been know
for some time that one of the most common sources of allergens in a
house is from dust mites. Of course, as is the case with all
allergens, only certain people are allergic to dust mite allergens.
But this group of people can be quite large in many areas,
especially in hot humid areas. For example, in the southeastern
United States of America, where it is both hot and humid for much
of the year, the incidence of house dust mite allergies in the
general population can be as high as 25%. House dust mites thrive
in plush carpets, overstuffed upholstery, cushy bed comforters and
the like. Methods
[0232] Mite dust allergen isolation--Dermatophagoides farinae are
the American house dust mite. D. farinae were cultured on a 1:1
ratio of Purina Laboratory Chow (Ralston Purina, Co, St. Louis,
Mo.) and Fleischmann's granulated dry yeast (Standard Brands, Inc.
New York, N.Y.) at room temperature and 75% humidity. Live mites
were aspirated from the culture container as they migrated from the
medium, killed by freezing, desiccated and stored at 0% humidity.
The allergenic component of the mite dust was extracted with water
at ambient temperature. Five-hundred mg of mite powder were added
to 5 mL of water (1:10 w/v) in a 15 mL conical centrifuge tube
(VWR, Rochester, N.Y.), shaken for one minute and allowed to stand
overnight at ambient temperature. The next day, the aqueous phase
was filtered using a 0.2 .mu.m disposable syringe filter (Nalgene,
Rochester, N.Y.). The filtrate was termed mite dust allergen and
used to test for induction of PGE.sub.2 biosynthesis in A549
pulmonary epithelial cells.
[0233] Cell culture and treatment--This experiment involved the
human airway epithelial cell line, A549 (American Type Culture
Collection, Bethesda, Md.). The cells were cultured and treated as
previously described in Example 2. Mite allergen was added to the
culture medium to achieve a final concentration of 1000 ng/mL.
Twenty-four hours later, the culture medium was sampled for
PGE.sub.2 concentration.
[0234] PGE.sub.2 assay--Determination of PGE.sub.2 in the culture
medium was performed as previously described in Example 1.
[0235] Statistical analysis--Means of eight replicates per
treatment were computed using Excels spreadsheets (Microsoft,
Redmond, Wash.).
Results
[0236] Mite allergen treatment increased PGE.sub.2 biosynthesis
6-fold in A549 cells relative to the solvent treated controls (FIG.
8).
EXAMPLE 22
Hops Derivatives Inhibit Mite Dust Allergen Activation of PGE.sub.2
Biosynthesis in A549 Pulmonary Cells
[0237] Summary--This example illustrates that hops derivatives are
capable of inhibiting the PGE.sub.2 stimulatory effects of mite
dust allergens in A549 pulmonary cells.
Methods
[0238] The cell line and testing procedures are as described in
Example 22. In addition to mite dust allergen, test materials
included Hops fractions (1) alpha hop (1% alpha acids; AA), (2)
aromahop OE (10% beta acids and 2% isomerized alpha acids , (3)
isohop (isomerized alpha acids; IAA), (4) beta acid solution (beta
acids BA), (5) hexahop gold (hexahydro isomerized alpha acids;
HHIAA), (6) redihop (reduced isomerized-alpha acids; RLAA), and (7)
tetrahop (tetrahydro-iso-alpha acids THIAA). Test materials at a
final concentration of 10 .mu.g/mL were added 60 minutes prior to
the addition of the mite dust allergen.
Results
[0239] Table 15 depicts the extent of inhibition of PGE.sub.2
biosynthesis by hops derivatives in A549 pulmonary cells stimulated
by mite dust allergen. All hops derivatives were capable of
significantly inhibiting the stimulatory effects of mite dust
allergens. TABLE-US-00014 TABLE 15 PGE.sub.2 inhibition by hops
derviatives in A549 pulmonary epithelial cells stimulated by mite
dust allergen Percent Inhibition of Test Material PGE.sub.2
Biosynthesis Alpha hop (AA) 81 Aromahop OE 84 Isohop (IAA) 78 Beta
acids (BA) 83 Hexahop (HHIAA) 82 Redihop (RIAA) 81 Tetrahop (THIAA)
76
[0240] In conclusion, it would also be useful to identify a natural
formulation of compounds that would inhibit expression of COX-2,
inhibit prostaglandin synthesis selectively in target cells, or
inhibit inflammation response selectively in target cells.
[0241] A preferred embodiment comprises compositions containing at
least one fraction isolated or derived from hops (Humulus lupulus).
Examples of fractions isolated or derived from hops are alpha
acids, isoalpha acids, reduced isoalpha acids, tetra-hydroisoalpha
acids, hexa-hydroisoalpha acids, beta acids, and spent hops.
Preferred compounds of fractions isolated or derived from hops,
include, but are not limited to, humulone, cohumulone, adhumulone,
isohumulone, isocohumulone, isoadhumulone, dihydro-isohumulone,
dihydro-isocohumulone, dihydro-adhumulone, tetrahydro-isohumulone,
tetrahydro-isocohumulone, tetrahydro-adhumulone,
hexahydro-isohumulone, hexahydro-isocohumulone, and
hexahydro-adhumulone. Preferred compounds can also bear
substituents, such as halogens, ethers, and esters.
[0242] Another embodiment comprises composition containing
tryptanthrin and conjugates thereof.
[0243] Other embodiments relate to combinations of components. One
embodiment relates to compositions that include, as a first
component, an active ingredient isolated or derived from an extract
of hops and as a second component at least one member selected from
the group consisting of rosemary (Rosmarinus officinalis L.), an
extract or compound derived from rosemary, a triterpene species or
derivatives or conjugates thereof, and tryptanthrin or conjugates
thereof. Another embodiment relates to compositions that include,
as a first component, tryptanthrin or conjugates thereof and as a
second component at least one member selected from the group
consisting of an active ingredient isolated or derived from an
extract of hops, rosemary, an extract or compound derived from
rosemary, and a triterpene species or derivatives or conjugates
thereof.
[0244] It will be readily apparent to those skilled in the art that
various changes and modifications of an obvious nature may be made
without departing from the spirit of the invention, and all such
changes and modifications are considered to fall within the scope
of the invention as defined by the appended claims. Such changes
and modifications would include, but not be limited to, the
incipient ingredients added to affect the capsule, tablet, lotion,
food or bar manufacturing process as well as vitamins, herbs,
flavorings and carriers. Other such changes or modifications would
include the use of other herbs or botanical products containing the
combinations of the preferred embodiments disclosed above. Many
additional modifications and variations of the embodiments
described herein may be made without departing from the scope, as
is apparent to those skilled in the art. The specific embodiments
described herein are offered by way of example only.
* * * * *