U.S. patent application number 11/081201 was filed with the patent office on 2005-11-10 for substituted piperidine compounds useful as modulators of chemokine receptor activity.
Invention is credited to Baxter, Andrew, Evans, Richard, Harden, David, Kindor, Nicholas, Marriott, David, McInally, Thomas, Perry, Matthew, Springthorpe, Brian, Thom, Stephen.
Application Number | 20050250792 11/081201 |
Document ID | / |
Family ID | 20416734 |
Filed Date | 2005-11-10 |
United States Patent
Application |
20050250792 |
Kind Code |
A1 |
Thom, Stephen ; et
al. |
November 10, 2005 |
Substituted piperidine compounds useful as modulators of chemokine
receptor activity
Abstract
The invention provides compounds of formula (I): 1 wherein
R.sup.1, R.sup.2, R.sup.3, R.sup.6, Z, Q, m, n, X.sup.1, X.sup.2,
X.sup.3, X.sup.4 and T are as defined in the specification,
processes for their preparation, pharmaceutical compositions
containing them, and their use in therapy, especially for the
treatment of chemokine receptor related diseases and
conditions.
Inventors: |
Thom, Stephen;
(Loughborough, GB) ; Baxter, Andrew;
(Loughborough, GB) ; Kindor, Nicholas;
(Loughborough, GB) ; McInally, Thomas;
(Loughborough, GB) ; Springthorpe, Brian;
(Loughborough, GB) ; Perry, Matthew;
(Loughborough, GB) ; Harden, David; (Loughborough,
GB) ; Evans, Richard; (Loughborough, GB) ;
Marriott, David; (Loughborough, GB) |
Correspondence
Address: |
FISH & RICHARDSON P.C.
P.O BOX 1022
MINNEAPOLIS
MN
55440-1022
US
|
Family ID: |
20416734 |
Appl. No.: |
11/081201 |
Filed: |
March 16, 2005 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
11081201 |
Mar 16, 2005 |
|
|
|
10069215 |
Mar 19, 2002 |
|
|
|
6903085 |
|
|
|
|
10069215 |
Mar 19, 2002 |
|
|
|
PCT/GB00/03179 |
Aug 18, 2000 |
|
|
|
Current U.S.
Class: |
514/256 ;
514/355; 544/329; 546/315 |
Current CPC
Class: |
A61P 17/14 20180101;
C07D 413/12 20130101; A61P 19/08 20180101; A61P 29/00 20180101;
A61P 19/02 20180101; A61P 37/00 20180101; C07D 405/12 20130101;
C07D 211/58 20130101; A61P 17/00 20180101; C07D 409/14 20130101;
C07D 413/14 20130101; C07D 417/12 20130101; A61P 11/02 20180101;
A61P 3/10 20180101; A61P 1/04 20180101; A61P 43/00 20180101; A61P
9/10 20180101; A61P 11/00 20180101; A61P 11/06 20180101; A61P 17/06
20180101; A61P 25/06 20180101; A61P 25/00 20180101; A61P 37/06
20180101; A61P 1/00 20180101; C07D 401/12 20130101; C07D 409/12
20130101; A61P 19/00 20180101 |
Class at
Publication: |
514/256 ;
514/355; 546/315; 544/329 |
International
Class: |
A61K 031/505; A61K
031/455 |
Foreign Application Data
Date |
Code |
Application Number |
Aug 24, 1999 |
SE |
9902987-8 |
Claims
1. A compound of formula (I): 174wherein Z is CR.sup.4R.sup.5, C(O)
or CR.sup.4R.sup.5-Z.sup.1; Z.sup.1 is C.sub.1-4 alkylene,
C.sub.2-4 alkenylene or C(O)NH; R.sup.1 represents a
C.sub.1-C.sub.12 alkyl group optionally substituted by one or more
substituents independently selected from cyano, hydroxyl,
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-7
cycloalkyl, C.sub.1-C.sub.6 alkoxycarbonyl and phenyl (itself
optionally substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents C.sub.2-C.sub.6 alkenyl optionally substituted
by phenyl (itself optionally substituted by one or more of halogen,
nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents a 3- to 14-membered saturated or unsaturated
ring system which optionally comprises up to two ring carbon atoms
that form carbonyl groups and which optionally further comprises up
to 4 ring heteroatoms independently selected from nitrogen, oxygen
and sulphur, wherein the ring system is optionally substituted by
one or more substituents independently selected from: halogen,
cyano, nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.- 1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.s- ub.6 alkyl),
aryl(C.sub.1-C.sub.6 alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.7R.sup.8,
--C(O)NR.sup.23R.sup.24, S(O).sub.2NR.sup.18R.sup.19,
S(O).sub.2R.sup.20, R.sup.25C(O), carboxyl, C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, oxo, hydroxy, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl),
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 haloalkoxy,
S(O).sub.2(C.sub.1-C.sub.6 alkyl), C(O)NH.sub.2, carboxy or
C.sub.1-C.sub.6 alkoxycarbonyl; m is 0 or 1; Q represents an oxygen
or sulphur atom or a group NR.sup.9, C(O), C(O)NR.sup.9,
NR.sup.9C(O) or CH.dbd.CH; n is 0, 1, 2, 3, 4, 5 or 6 provided that
when n is 0, then m is 0; each R.sup.2 and R.sup.3 independently
represents a hydrogen atom or a C.sub.1-C.sub.4 alkyl group, or
(CR.sup.2R.sup.3).sub.n represents C.sub.3-C.sub.7 cycloalkyl
optionally substituted by C.sub.1-C.sub.4 alkyl; T represents a
group NR.sup.10, C(O)NR.sup.10, NR.sup.11C(O)NR.sup.10 or
C(O)NR.sup.10NR.sup.11; X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are,
independently, CH.sub.2, CHR.sup.12 {wherein each R.sup.12 is,
independently, C.sub.1-C.sub.4 alkyl or C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl)} or C.dbd.O; or, when they are
CHR.sup.12, the R.sup.12 groups of X.sup.1 and X.sup.3 or X.sup.4,
or, X.sup.2 and X.sup.3 or X.sup.4 join to form a two or three atom
chain which is CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2,
CH.sub.2OCH.sub.2 or CH.sub.2SCH.sub.2; provided always that at
least two of X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are CH.sub.2;
R.sup.4 and R.sup.5 each independently represent a hydrogen atom or
a C.sub.1-C.sub.4 alkyl group; R.sup.6 is aryl or heterocyclyl,
both optionally substituted by one or more of: halogen, cyano,
nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2,
C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6 C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.16R.sup.17,
--C(O)NR.sup.21R.sup.22, S(O).sub.2NR.sup.13R.sup.14- ,
S(O).sub.2R.sup.15, R.sup.26C(O), carboxyl, C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl; R.sup.7,
R.sup.8, R.sup.9, R.sup.10, R.sup.11, R.sup.13, R.sup.14, R.sup.16,
R.sup.17, R.sup.18, R.sup.19, R.sup.21, R.sup.22, R.sup.23 and
R.sup.24 are, independently hydrogen, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6 hydroxyalkyl,
C.sub.3-C.sub.7 cycloalkyl, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl) or phenyl(C.sub.1-C.sub.6 alkyl);
and, R.sup.15 and R.sup.20 are, independently, C.sub.1-C.sub.6
alkyl, C.sub.1-C.sub.6 hydroxyalkyl, C.sub.3-C.sub.6 cycloalkyl,
C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.4 alkyl) or
C.sub.1-C.sub.6 alkyl optionally substituted by phenyl; R.sup.25
and R.sup.26 are, independently, C.sub.1-C.sub.6 alkyl or phenyl
(optionally substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or a
pharmaceutically acceptable salt thereof, or solvate thereof, or a
solvate of a salt thereof; provided that when T is C(O)NR.sup.10
and R.sup.1 is optionally substituted phenyl then n is not 0.
2. A compound according to claim 1, wherein Q represents a sulphur
atom or a group NH, C(O) or NHC(O).
3. A compound according to claim 1, wherein T represents a group
NH, C(O)NH or NHC(O)NH.
4. A compound according to claim 1, wherein X.sup.1, X.sup.2,
X.sup.3 and X.sup.4 are all CH.sub.2.
5. A compound as defined in any one of Examples 1 to 416.
6. A process for the preparation of a compound of formula (I) as
defined in claim 1 which comprises: (a) when n is at least 1, the
CR.sup.2R.sup.3 group attached directly to T is CHR.sup.3 and T is
NR.sup.10, reacting a compound of general formula 175wherein n' is
0 or an integer from 1 to 3 and R.sup.1, R.sup.2, R.sup.3, m and Q
are as defined in formula (I), with a compound of general formula
176or a salt thereof, wherein X.sup.1, X.sup.2, X.sup.3, X.sup.4,
Z, R.sup.6 and R.sup.10 are as defined in formula (I), in the
presence of a reducing agent; or (b) when n is at least 1, the
CR.sup.2R.sup.3 group attached directly to T is C(C.sub.1-C.sub.4
alkyl).sub.2 and T is NR.sup.10, reacting a compound of general
formula 177wherein n' is 0 or an integer from 1 to 3, R.sup.2' and
R.sup.3' each independently represent a C.sub.1-C.sub.4 alkyl
group, and R.sup.1, R.sup.2, R.sup.3, R.sup.10, m and Q are as
defined in formula (I), with a compound of general formula
178wherein X.sup.1, X.sup.2, X.sup.3, X.sup.4, Z and R.sup.6 are as
defined in formula (I), in the presence of a reducing agent; or (c)
when T is C(O)NR.sup.10, reacting a compound of general formula
179wherein R.sup.1, R.sup.2, R.sup.3, Q, m and n are as defined in
formula (I), with a compound of formula (III) or a salt thereof as
defined in (a) above; or (d) when m is 1 and Q is NR.sup.9,
reacting a compound of general formula (VII), R.sup.1-L.sup.1,
wherein L.sup.1 represents a leaving group (e.g. a halogen atom)
and R.sup.1 is as defined in formula (I), with a compound of
general formula 180or a salt thereof, wherein n, T, X.sup.1,
X.sup.2, X.sup.3, X.sup.4, Z, R.sup.2, R.sup.3, R.sup.6 and R.sup.9
are as defined in formula (I); or (e) when at least one of R.sup.4
and R.sup.5 represents a hydrogen atom, reacting a compound of
general formula 181or a salt thereof, wherein R.sup.1, R.sup.2,
R.sup.3, Q, m, n, X.sup.1, X.sup.2, X.sup.3, X.sup.4 and T are as
defined in formula (I), with a compound of general formula (X),
R.sup.6--C(O)--R.sup.20, wherein R.sup.20 represents a hydrogen
atom or a C.sub.1-C.sub.4 alkyl group and R.sup.6 is as defined in
formula (I), in the presence of a reducing agent; or (f) reacting a
compound of formula (IX) as defined in (e) above, with a compound
of general formula 182wherein L.sup.2 represents a leaving group
(e.g. a halogen atom) and Z and R.sup.6 are as defined in formula
(I); or (g) when T is NR.sup.10, reacting a compound of general
formula R.sup.1-(Q).sub.m-(CR.sup.2R.sup.3).sub.n-L.sup.3 (XII)
wherein L represents a leaving group (e.g. a halogen atom) and
R.sup.1, R.sup.2, R.sup.3, m, n and Q are as defined in formula
(I), with a compound of formula (III) or a salt thereof as defined
in (a) above; or (h) when T is NHC(O)NR.sup.10, reacting a compound
of general formula
R.sup.1-(Q).sub.m-(CR.sup.2R.sup.3).sub.n--N.dbd.C.dbd.O (XIII)
wherein R.sup.1, R.sup.2, R.sup.3, Q, m and n are as defined in
formula (I), with a compound of formula (III) or a salt thereof as
defined in (a) above; or (i) when T is C(O)NH, Z is CH.sub.2, n is
1, R.sup.2 and R.sup.3 are hydrogen or C.sub.1-C.sub.4 alkyl and Q
is oxygen or sulphur, reacting a compound of formula (XIV):
183wherein Hal is a suitable halogen, R.sup.2, R.sup.3, X.sup.1,
X.sup.2, X.sup.3, X.sup.4, Z and R.sup.6 are as defined in formula
(I), with R.sup.1OH or R.sup.1SH in the presence of a suitable
base; and optionally after (a), (b), (c), (d), (e), (f), (g), (h)
or (i) forming a pharmaceutically acceptable salt or solvate of the
compound of formula (I) obtained.
7. A pharmaceutical composition comprising a compound of formula
(I), or a pharmaceutically acceptable salt or solvate thereof, as
claimed in claim 1 and a pharmaceutically acceptable adjuvant,
diluent or carrier.
8. A process for the preparation of a pharmaceutical composition as
claimed in claim 7 which comprises mixing a compound of formula
(I), or a pharmaceutically acceptable salt or solvate thereof, with
a pharmaceutically acceptable adjuvant, diluent or carrier.
9. (canceled)
10. A method of treating a disease wherein the modulation of a
chemokine receptor is beneficial comprising administering a
compound of formula (I), 184wherein Z is CR.sup.4R.sup.5, C(O) or
CR.sup.4R.sup.5-Z.sup.l; Z.sup.1 is C.sub.1-4 alkylene, C.sub.2-4
alkenylene or C(O)NH; R.sup.1 represents a C.sub.1-C.sub.12 alkyl
group optionally substituted by one or more substituents
independently selected from cyano, hydroxyl, C.sub.1-C.sub.6
alkoxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-7 cycloalkyl,
C.sub.1-C.sub.6 alkoxycarbonyl and phenyl (itself optionally
substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents C.sub.2-C.sub.6 alkenyl optionally substituted
by phenyl (itself optionally substituted by one or more of halogen,
nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents a 3- to 14-membered saturated or unsaturated
ring system which optionally comprises up to two ring carbon atoms
that form carbonyl groups and which optionally further comprises up
to 4 ring heteroatoms independently selected from nitrogen, oxygen
and sulphur, wherein the ring system is optionally substituted by
one or more substituents independently selected from: halogen,
cyano, nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-.sub.6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2,
C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.7R.sup.8,
--C(O)NR.sup.23R.sup.24, S(O).sub.2NR.sup.18R.sup.19,
S(O).sub.2R.sup.20, R.sup.25C(O), carboxyl, C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, oxo, hydroxy, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl),
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 haloalkoxy,
S(O).sub.2(C.sub.1-C.sub.6 alkyl), C(O)NH.sub.2, carboxy or
C.sub.1-C.sub.6 alkoxycarbonyl; m is 0 or 1; Q represents an oxygen
or sulphur atom or a group NR.sup.9, C(O), C(O)NR.sup.9,
NR.sup.9C(O) or CH.dbd.CH; n is 0, 1, 2, 3, 4, 5 or 6 provided that
when n is 0, then m is 0; each R.sup.2 and R.sup.3 independently
represents a hydrogen atom or a C.sub.1-C.sub.4 alkyl group, or
(CR.sup.2R.sup.3).sub.n represents C.sub.3-C.sub.7 cycloalkyl
optionally substituted by C.sub.1-C.sub.4 alkyl; T represents a
group NR.sup.10, C(O)NR.sup.10, NR.sup.11C(O)NR.sup.10 or
C(O)NR.sup.10NR.sup.11; X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are,
independently, CH.sub.2, CHR.sup.12 {wherein each R.sup.12 is,
independently, C.sub.1-C.sub.4 alkyl or C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl)} or C.dbd.O; or, when they are
CHR.sup.12, the R.sup.12 groups of X.sup.1 and X.sup.3 or X.sup.4,
or, X.sup.2 and X.sup.3 or X.sup.4 join to form a two or three atom
chain which is CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2,
CH.sub.2OCH.sub.2 or CH.sub.2SCH.sub.2; provided always that at
least two of X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are CH.sub.2;
R.sup.4 and R.sup.5 each independently represent a hydrogen atom or
a C.sub.1-C.sub.4 alkyl group; R.sup.6 is aryl or heterocyclyl,
both optionally substituted by one or more of: halogen, cyano,
nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2,
C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.16R.sup.17,
--C(O)NR.sup.21R.sup.22, S(O).sub.2NR.sup.13R.sup.14,
S(O).sub.2R.sup.15, R.sup.26C(O), carboxyl, C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl; R.sup.7,
R.sup.8, R.sup.9, R.sup.10, R.sup.11, R.sup.13, R.sup.14, R.sup.16,
R.sup.17, R.sup.18, R.sup.19, R.sup.21, R.sup.23 and R.sup.24 are,
independently hydrogen, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl, C.sub.1-C.sub.6 hydroxyalkyl, C.sub.3-C.sub.7
cycloalkyl, C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.4 alkyl) or
phenyl(C.sub.1-C.sub.6 alkyl); and, R.sup.15 and R.sup.20 are,
independently, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 hydroxyalkyl,
C.sub.3-C.sub.6 cycloalkyl, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl) or C.sub.1-C.sub.6 alkyl
optionally substituted by phenyl; R.sup.25 and R.sup.26 are,
independently, C.sub.1-C.sub.6 alkyl or phenyl (optionally
substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or a
pharmaceutically acceptable salt thereof, or solvate thereof, or a
solvate of a salt thereof.
11. The method of claim 10, comprising treating an inflammatory
disease in a patient suffering from, or at risk of, said disease,
which comprises administering to the patient a therapeutically
effective amount of a compound of formula (I), or a
pharmaceutically acceptable salt thereof, or solvate thereof, or a
solvate of a salt thereof.
Description
[0001] The present invention relates to substituted piperidine
compounds, processes for their preparation, pharmaceutical
compositions containing them and their use in therapy.
[0002] Chemokines play an important role in immune and inflammatory
responses in various diseases and disorders, including asthma and
allergic diseases, as well as autoimmune pathologies such as
rheumatoid arthritis and atherosclerosis. These small secreted
molecules are a growing superfamily of 8-14 kDa proteins
characterised by a conserved four cysteine motif. The chemokine
superfamily can be divided into two main groups exhibiting
characteristic structural motifs, the Cys-X-Cys (C-X-C) and Cys-Cys
(C-C) families. These are distinguished on the basis of a single
amino acid insertion between the NH-proximal pair of cysteine
residues and sequence similarity.
[0003] The C-X-C chemokines include several potent chemoattactants
and activators of neutrophils such as interleukin-8 (IL-8) and
neutophil-activating peptide 2 (NAP-2).
[0004] The C-C chemokines include potent chemoattractants of
monocytes and lymphocytes but not neutrophils such as human
monocyte chemotactic proteins 1-3 (MCP-1, MCP-2 and MCP-3), RANTES
(Regulated on Activation, Normal T Expressed and Secreted), eotaxin
and the macrophage inflammatory proteins 1.alpha. and 1.beta.
(MIP-1.alpha. and MIP-1.beta.).
[0005] Studies have demonstrated that the actions of the chemokines
are mediated by subfamilies of G protein-coupled receptors, among
which are the receptors designated CCR1, CCR2, CCR2A, CCR2B, CCR3,
CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCR10, CXCR1, CXCR2, CXCR3 and
CXCR4. These receptors represent good targets for drug development
since agents which modulate these receptors would be useful in the
treatment of disorders and diseases such as those mentioned
above.
[0006] The present invention provides a compound of formula (I):
2
[0007] wherein
[0008] Z is CR.sup.4R.sup.5, C(O) or CR.sup.4R.sup.5-Z.sup.1;
[0009] Z.sup.1 is C.sub.1-4 alkylene (such as CH.sub.2), C.sub.2-4
alkenylene (such as CH.dbd.CH) or C(O)NH;
[0010] R.sup.1 represents a C.sub.1-C.sub.12 allyl group optionally
substituted by one or more substituents independently selected from
cyano, hydroxyl, C.sub.1-C.sub.6 alkoxy (such as methoxy or
ethoxy), C.sub.1-C.sub.6 alkylthio (such as methylthio), C.sub.3-7
cycloalkyl (such as cyclopropyl), C.sub.1-C.sub.6 alkoxycarbonyl
(such as methoxycarbonyl) and phenyl (itself optionally substituted
by one or more of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl (such as CF.sub.3),
phenyl(C.sub.1-C.sub.6 alkyl) (such as benzyl), C.sub.1-C.sub.6
alkoxy, C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6
alkyl), C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycabonyl);
or
[0011] R.sup.1 represents C.sub.2-C.sub.6 alkenyl optionally
substituted by phenyl (itself optionally substituted by one or more
of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
[0012] R.sup.1 represents a 3- to 14-membered saturated or ring
system which optionally comprises up to two ring carbon atoms that
form carbonyl groups and which optionally further comprises up to 4
ring heteroatoms independently selected from nitrogen, oxygen and
sulphur, wherein the ring system is optionally substituted by one
or more substituents independently selected from halogen, cyano,
nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.- 1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclS(O).sub.2(C.sub.1-C.sub- .6 alkyl) aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.su- b.6 alkyl)S(O).sub.2,
C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.1-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.7R.sup.8,
--C(O)NR.sup.23R.sup.24, S(O).sub.2NR.sup.18R.sup.19,
S(O).sub.2R.sup.20, R.sup.25C(O), carboxyl, C.sub.1-C.sub.6
alkoxycarbonyl aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, oxo, hydroxy, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl),
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 haloalkoxy,
S(O).sub.2(C.sub.1-C.sub.6 alkyl), C(O)NH.sub.2, carboxy or
C.sub.1-C.sub.6 alkoxycarbonyl;
[0013] m is 0 or 1;
[0014] Q represents an oxygen or sulphur atom or a group NR.sup.9,
C(O), C(O)NR.sup.9, NR.sup.9C(O) or CH.dbd.CH;
[0015] n is 0, 1, 2, 3, 4, 5 or 6 provided that when n is 0, then m
is 0;
[0016] each R.sup.2 and R.sup.3 independently represents a hydrogen
atom or a C.sub.1-C.sub.4 alkyl group, or (CR.sup.2R.sup.3).sub.n
represents C.sub.3-C.sub.7 cycloalkyl optionally substituted by
C.sub.1-C.sub.4 alkyl;
[0017] T represents a group NR.sup.10, C(O)NR.sup.10,
NR.sup.11C(O)NR.sup.10 or C(O)NR.sup.10NR.sup.11;
[0018] X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are, independently,
CH.sub.2, CHR.sup.12 {wherein each R.sup.12 is, independently,
C.sub.1-C.sub.4 alkyl or C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.4
alkyl)} or C.dbd.O; or, when they are CHR.sup.12, the R.sup.12
groups of X.sup.1 and X.sup.3 or X.sup.4, or, X.sup.2 and X.sup.3
or X.sup.4 join to form a two or three atom chain which is
CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.su- b.2OCH.sub.2 or
CH.sub.2SCH.sub.2; provided always that at least two of X.sup.1,
X.sup.2, X.sup.3 and X.sup.4 are CH.sub.2;
[0019] R.sup.4 and R.sup.5 each independently represent a hydrogen
atom or a C.sub.1-C.sub.4 alkyl group;
[0020] R.sup.6 is aryl or heterocyclyl, both optionally substituted
by one or more of: halogen, cyano, nitro, oxo, hydroxyl,
C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6 hydroxyalkyl,
C.sub.1-C.sub.6 haloalkyl, C.sub.1-6 alkoxy(C.sub.1-C.sub.6 alkyl),
C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), hetcocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2,
C.sub.1-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocycyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.16R.sup.17,
--C(O)NR.sup.21R.sup.22, S(O).sub.2NR.sup.13R.sup.14,
S(O).sub.2R.sup.15, R.sup.26C(O), carboxyl C.sub.1-C.sub.6
alkoxycarbonyl, aryl and hetercyclyl; wherein the foregoing aryl
and hetercyclyl moieties are optionally substituted by one or more
of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl;
[0021] R.sup.7, R.sup.8, R.sup.9, R.sup.10, R.sup.11, R.sup.13,
R.sup.14, R.sup.16, R.sup.17, R.sup.18, R.sup.19, R.sup.21,
R.sup.22, R.sup.23, and R.sup.24 are, independently hydrogen,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.3-C.sub.7 cycloalkyl, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl) or phenyl(C.sub.1-C.sub.6 alkyl);
and,
[0022] R.sup.15 and R.sup.20 are, independently, C.sub.1-C.sub.6
alkyl, C.sub.1-C.sub.6 hydroxyalkyl, C.sub.3-C.sub.6 cycloalkyl,
C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.4 alkyl) or
C.sub.1-C.sub.6 alkyl optionally substituted by phenyl;
[0023] R.sup.25 and R.sup.26 are, independently, C.sub.1-C.sub.6
alkyl or phenyl (optionally substituted by one or more of halogen,
nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl);
[0024] or a pharmaceutically acceptable salt thereof, or solvate
thereof, or a solvate of a salt thereof;
[0025] provided that when T is C(O)NR.sup.10 and R.sup.1 is
optionally substituted phenyl then n is not 0.
[0026] Certain compounds of formula (I) are capable of existing in
isomeric forms (for example as tautomers, enantiomers, geometric
isomers or diastereomers). The present invention encompasses all
such isomers and mix thereof in all proportions.
[0027] Hydroxyalkyl is, for example, 2-hydoxyeth-1-yl. Haloalkyl
is, for example, CF.sub.3. Alkoxy is, for example, methoxy or
ethoxy. Alkoxy(C.sub.1-C.sub.6 alkyl) is, for example,
methoxymethyl or ethoxyethyl. Cycloalkyl is, for example,
cyclopropyl or cyclohexyl. Cycloalkyl(C.sub.1-C.sub.6 alkyl) is,
for example, cyclopropylmethyl. Alkylthio is, for example,
methylthio or ethylthio. Alkylthio(C.sub.1-C.sub.6 alkyl) is, for
example, methylthiomethyl. Alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl)
is, for example, CH.sub.3C(O)OCH.sub.2. S(O).sub.2(alkyl) is, for
example, CH.sub.3S(O).sub.2. AlkylS(O).sub.2(C.sub.1-C.sub.6 alkyl)
is, for example, CH.sub.3S(O).sub.2CH.sub.2. Aryl(C.sub.1-C.sub.6
alkyl) is, for example, benzyl, 2-phenyleth-1-yl or
1-phenyleth-1-yl. Hetrocyclyl(C.sub.1-C.sub.6 alkyl) is, for
example, heterocyclylmethyl. ArylS(O).sub.2(C.sub.1-C.sub.6 alkyl)
is, for example, phenylS(O).sub.2CH.sub.2.
HeterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl) is, for example,
heterocyclylS(O).sub.2CH.sub.2. Aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2 is, for example, benzylS(O).sub.2.
Heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2 is, for example,
heterocyclylCH.sub.2S(O).sub.2. Alkenyl is, for example, vinyl or
allyl. Carboxy-substituted C.sub.1-C.sub.6 alkoxy is, for example,
HOC(O)CH.sub.2CH.sub.2O. Haloalkoxy is, for example, OCF.sub.3.
Hydroxyalkoxy is, for example, HOCH.sub.2CH.sub.2O.
Alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy is, for example,
CH.sub.3OC(O)CH.sub.2CH.sub.2O. Aryloxy is, for example,
phenoxy.
[0028] Heterocyclyloxy is, for example, pyridinyloxy or
pyrimidinyloxy. C.sub.3-C.sub.7 cycloalkyl(C.sub.1-C.sub.6
alkylthio) is, for example, cyclopropylCH.sub.2S. Alkynylthio is,
for example, propargylthio. Alkylcarbonylamino is, for example,
acylamino. Haloalkylcarbonylamino is, for example,
ClCH.sub.2C(O)NH. Alkoxycarbonyl is, for example,
CH.sub.3OC(O).
[0029] Aryl is a carbocyclic aromatic ring optionally fused to one
or more carbocyclic rings. Aryl is, for example, phenyl, naphthyl
or indanyl.
[0030] Heterocyclyl is an aromatic or non-aromatic ring system
preferably comprising up to 6 (preferably up to 4) heteroatoms
selected from the group comprising nitrogen, oxygen and sulphur,
and preferably comprising one, two or three 5- or 6-membered rings.
Heterocyclyl is, for example, furyl, thienyl pyrrolyl,
2,5-dihydropyrrolyl, thiazolyl, pyrazolyl, oxazolyl, isoxazolyl,
imidazolyl, 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,3-triazolyl,
1,2,4-triazolyl, tetratolyl, pyrrolidinyl, piperidinyl,
piperazinyl, morpholinyl, pyridinyl (for example 2-pyridinyl,
3-pyridinyl or 4-pyridinyl), pyrimidiyl (for example 2-pyrimidinyl
or 4-pyrimidinyl), pyrazinyl, pyridazinyl, indolyl,
2,3-dihydroindolyl, benzo[b]furyl, benz[b]thienyl,
2,3-dihydrobenz[b]thienyl (for example
1-dioxo-2,3-dihydrobenz[b]thienyl), benzimidazolyl, benzatriazolyl,
benzoxazolyl, benzthiazolyl, 2,3-dihydrobenzthiazolyl (for example
2,3-dihydrobenzthiazol-2-onyl which is also known as
2-oxo-1,3-benzothiazol-3(2H)-yl), 1,2,3-benzothiadiazolyl,
1,2,3-benzoxadiazolyl, 2,1,3-benzothiadiazolyl,
2,1,3-benzoxadiazolyl, quinoxalinyl dihydro-1-benzopyryliumyl (for
example a coumarinyl or a chromenonyl), 1,3-benzodioxolyl (also
known as 1,2-methylenedioxyphenyl),
3,4-dihydro-1H-2,1-benzothiazinyl (for example
2-dioxo-3,4-dihydro-1H-2,1 -benzothiazinyl), purine (for example
1H-purine or 9H-purine), 1H-pyrazolo[3,4-d]pyrimidinyl,
thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, quinolinyl (for
example 2-quinolinyl, 3-quinolinyl or 4-quinolinyl), isoquinolinyl,
quinazolinyl or dibenzothiophenyl; or a ring as shown below: 3
[0031] The group R.sup.1 may represent an optionally substituted 3-
to 14-membered (especially 5- to 10-membered) saturated or
unsaturated ring system which optionally comprises one or two ring
carbon atoms that form carbonyl groups and which optionally further
comprises one, two, three or four ring heteroatoms independently
selected from nitrogen, oxygen and sulphur. Examples of R.sup.1
ring systems, which can be moncyclic or polycyclic, include
cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl, naphthyl,
indanyl, furyl, thienyl, pyrrolyl, 2,5-dihydropyrrolyl, thiazolyl,
pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, 1,2,4-oxadiazlyl,
1,3,4-oxadiazolyl, 1,2,3-triazolyl, 1,2,4-triazolyl, tetrazolyl,
pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, pyridinyl (for
example 2-pyridinyl, 3-pyridinyl or 4-pyridinyl), pyrimidinyl (for
example 2-pyrimidinyl or 4-pyrimidinyl), pyrazinyl, pyridazinyl,
indolyl, 2,3-dihydroindolyl, benzo[b]furyl, benz[b]thienyl,
2,3-dihydrobenz[b]thienyl (for example
1-dioxo-2,3-dihydrobenz[b]thienyl)- , benzimidazolyl,
benztriazolyl, benzoxazolyl, benzthiazolyl,
2,3-dihydrobenzthiazolyl (for example 2,3-dihydrobenzthiazol-2-onyl
which is also known as 2-oxo-1,3-benzothiazol-3(2H)-yl),
1,2,3-benzothiadiazolyl, 1,2,3-benzoxadiazolyl,
2,1,3-benzothiadiazolyl, 2,1,3-benzoxadiazolyl, quinoxalinyl,
dihydro-1-benzopyryliumyl (for example a coumarinyl or a
chromenonyl), 1,3-benzodioxolyl (also known as
1,2-methylenedioxyphenyl), 3,4-dihydro-1H-2,1-benzothiazinyl (for
example 2-dioxo-3,4-dihydro-1H-2,1-benzothiazinyl), purine (for
example 1H-purine or 9H-purine), 1H-pyrazolo[3,4-d]pyrimidinyl,
thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, quinolinyl (for
example 2qunolinyl, 3-quinolinyl or 4-quinolinyl), isoquinolnyl,
quinazolinyl or dibenzothiophenyl; or a ring as shown below: 4
[0032] In one aspect the present invention provides a compound of
formula (Ia): 5
[0033] wherein
[0034] R.sup.1 represents a C.sub.1-C.sub.12 alkyl group optionally
substituted by one or more substituents independently selected from
cyano, hydroxyl, C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 alkylthio
and C.sub.1-C.sub.6 alkoxycarbonyl, or
[0035] R.sup.1 is a 3- to 10-membered saturated or unsaturated ring
system which optionally comprises up to two ring carbon atoms that
form carbonyl groups and which optionally further comprises up to 4
ring heteroatoms independently selected from nitrogen, oxygen and
sulphur, wherein the ring system is optionally substituted by one
or more substituents independently selected from halogen, cyano,
nitro, hydroxyl, carboxyl, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
alkylthio, C.sub.1-C.sub.6 alkylthiomethyl, C.sub.1-C.sub.6
alkylcarbonylamino, --NR.sup.7R.sup.8, --C(O)NR.sup.7R.sup.8,
C.sub.1-C.sub.6 alkylcarbonyloxymethyl, C.sub.1-C.sub.6
alkoxycarbonyl, C.sub.1-C.sub.6 alkoxycarbonylpiperaanyl, furyl,
phenyl, pyridinyl, pyrazinyl, halophenyl, thienyl, thienylmethyl,
C.sub.1-C.sub.6 alkylbenzyl and 6
[0036] m is 0 or 1;
[0037] Q represents an oxygen or sulphur atom or a group NR.sup.9,
C(O), C(O)NR.sup.9 or NR.sup.9C(O);
[0038] n is 0, 1, 2, 3 or 4, provided that when n is 0, then m is
0;
[0039] each R.sup.2 and R.sup.3 independently represents a hydrogen
atom or a C.sub.1-C.sub.4 alkyl group;
[0040] T represents a group NR.sup.10, C(O)NR.sup.10 or
NR.sup.11C(O)NR.sup.10;
[0041] each X independently represents a group CH.sub.2, CHR.sup.12
or C.dbd.O, provided that at least two groups X simultaneously
represent CH.sub.2;
[0042] R.sup.4 and R.sup.5 each independently represent a hydrogen
atom or a C.sub.1-C.sub.4 alkyl group;
[0043] R.sup.6 represents a phenyl group optionally substituted by
one or more substituents independently selected from halogen, amino
(--NH.sub.2), nitro, cyano, sulphonyl (--SO.sub.3H), sulphonamido
(--SO.sub.2NH.sub.2), C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkoxy and C.sub.1-C.sub.6 alkylsulphonyl;
[0044] R.sup.7 and R.sup.8 each independently represent a hydrogen
atom or a group selected from C.sub.1-C.sub.6 hydroxyalkyl,
C.sub.3-C.sub.6 cycloalkyl and C.sub.1-C.sub.6 alkyl optionally
substituted by phenyl; R.sup.9, R.sup.10 and R.sup.11 each
independently represent a hydrogen atom, or a C.sub.1-C.sub.4 alkyl
or cyclopropylmethyl group; and
[0045] each R.sup.12 independently represents a C.sub.1-C.sub.4
alkyl or cyclopropylmethyl group;
[0046] or a pharmaceutically acceptable salt or solvate thereof
[0047] In the context of the present specification, unless
otherwise indicated an alkyl substituent or an alkyl moiety in a
substituent group may be linear or branched. Examples of alkyl
groups/moieties containing up to twelve carbon atoms include
methyl, ethyl n-propyl, iso propyl, n-butyl, isobutyl tert-butyl
n-pentyl n-hexyl, n-heptyl, n-octyl, n-nonyl n-decyl n-undecyl and
n-dodecyl groups. A C.sub.1-C.sub.6 hydroxyalkyl group will
comprise at least one hydroxyl group (e.g. one, two or three
hydroxyl groups) which may be attached to an internal or terminal
carbon atom of the alkyl chain. Similarly, a carboxy-substituted
C.sub.1-C.sub.6 alkoxy group will comprise at least one carboxyl
group (e.g. one, two or three carboxyl groups) which may be
attached to an internal or terminal carbon atom of the alkyl chain.
A C.sub.1-C.sub.6 haloalkyl or C.sub.1-C.sub.6 haloalkoxy group
will comprise at least one halogen atom (e.g. one, two, three or
four halogen atoms independently selected from fluorine, chlorine,
bromine and iodine) which may be attached to an internal or
terminal carbon atom of the alkyl chain. A halophenyl group will
comprise from 1 to 5 halogen atoms independently selected from
fluorine, chlorine, bromine and iodine. A C.sub.1-C.sub.6
alkylbenzyl group will comprise at least one C.sub.1-C.sub.6 alkyl
group (e.g. one, two or three C.sub.1-C.sub.6 alkyl groups)
attached to the phenyl ring of the benzyl moiety. If there is more
than one C.sub.1-C.sub.6 alkyl group attached to the phenyl ring,
the groups may be the same or different. In a C.sub.1-C.sub.6
alkoxycarbonylpiperazinyl substituent group, the piperazinyl moiety
is attached through a nitrogen atom to the carbonyl moiety. When T
represents C(O)NR.sup.9, it should be understood that the nitrogen
atom is attached directly to the six-membered heterocyclic ring in
formula (I).
[0048] The group R.sup.1 may represent a C.sub.1-C.sub.12,
preferably C.sub.1-C.sub.10, more preferably C.sub.1-C.sub.6, alkyl
group optionally substituted by one or more (e.g. one, two, three
or four) substituents independently selected from cyano, hydroxyl,
C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4, alkoxy,
C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4, alkylthio and
C.sub.1-C.sub.6 alkoxycarbonyl, preferably C.sub.1-C.sub.4
alkoxycarbonyl.
[0049] The group R.sup.1 may alternatively represent an optionally
substituted 3- to 10-membered saturated or unsaturated ring system
which optionally comprises one or two ring carbon atoms that form
carbonyl groups and which optionally further comprise one, two,
three or four ring heteroatoms independently selected from
nitrogen, oxygen and sulphur. Examples of ring systems that may be
used which can be moncyclic or polycyclic include cyclopropyl,
cyclobutyl, cyclopentyl, cyclohexyl, phenyl, pyrazolyl, furyl,
thienyl, imidazolyl, quinolinyl (e.g. 2-quinolinyl, 3-quinolinyl or
4-quinolinyl), pyridinyl (e.g. 2-pyridinyl, 3-pyridinyl or
4-pyridinyl), 1,3-benzodioxolyl, thiazolyl, benzimidazolyl,
oxadiazolyl (e.g. 1,2,4-oxadiazolyl), triazolyl (such as
1,2,3-triazolyl or 1,2,4-triazolyl), benzothiazolyl, pyrimidinyl
(e.g. 2-pyrimidinyl or 4-pyrimidinyl), benzothienyl, 7
[0050] In a further aspect of the invention the ring system of
R.sup.1 may be optionally substituted by one or more (e.g. one,
two, three or four) substituents independently selected from
halogen (e.g. fluorine, chlorine, bromine or iodine); cyano; nitro;
hydroxyl; carboxyl; C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4,
alkyl (especially methyl or ethyl); C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, hydroxyalkyl; C.sub.1C.sub.6, preferably
C.sub.1-C.sub.4, haloalkyl (e.g. trifluoromethyl); C.sub.1-C.sub.6,
preferably C.sub.1-C.sub.4, alkoxy (especially methoxy, ethoxy,
n-propoxy or isopropoxy); carboxy-substituted C.sub.1-C.sub.4,
preferably C.sub.1C.sub.4, alkoxy; C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, alkylthio (especially methylthio, ethylthio,
n-propylthio and tert-butylthio); C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, alkylthiomethyl particularly methylthiomethyl);
C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4, alkylcarbonylamino
(especially methylcabonylamino); --NR.sup.7R.sup.8;
--C(O)NR.sup.7R.sup.8; C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4,
alkylcarbonyloxymethyl (particularly methylcarbonyloxymethyl);
C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4, alkoxycarbonyl
(especially methoxycarbonyl or ethoxycarbonyl); C.sub.1-C.sub.6,
preferably C.sub.1C.sub.4, alkoxycarbonylpiperazinyl; furyl;
phenyl; pyridinyl; pyrazinyl; halophenyl (especially chlorophenyl);
thienyl; thienylmethyl; C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, alkylbenzyl (particularly methylbenzyl); and 8
[0051] In a further aspect R.sup.1 is an aromatic 5-membered
heterocyclyl having 2, 3 or 4 ring nitrogen atoms (for example
1,2,4-triazole, 1,2,4-oxadiazole, 1,3,4-oxadiazole or tetrazole)
substituted by one heteroaromatic ring (such as pyridine or
pyrazole) which is itself optionally substituted by halogen or
C.sub.1-C.sub.4 alkyl; or R.sup.1 is halophenyl (for example phenyl
optionally substituted (such as in the 4-position) by fluoro or
chloro; such as 4-chlorophenyl or 4-fluorophenyl).
[0052] In a further aspect of the invention Q is oxygen or m is 0.
In another aspect of the invention Q represents a sulphur atom or a
group NH, C(O) or NHC(O).
[0053] In yet another aspect of the invention n is 1 or 2.
[0054] In a further aspect of the invention T represents a group
NH, C(O)NH or NHC(O)NH. In another aspect of the invention T
repents a NH or C(O)NH group. In a further aspect T is C(O)NH.
[0055] In one aspect X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are all
CH.sub.2 or CHR.sup.12, wherein the R.sup.12 groups of X.sub.1 and
X.sup.3 or X.sup.4, or, X.sup.2 and X.sup.3 or X.sup.4 join to form
CH.sub.2CH.sub.2; provided always that at least two of X.sup.1,
X.sup.2, X.sup.3 and X.sup.4 are CH.sub.2. In a still further
aspect X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are al CH.sub.2.
Preferably, all four groups X represent CH.sub.2.
[0056] It is preferred that each R.sup.2 and R.sup.3 independently
represents a hydrogen atom or a methyl group, especially a hydrogen
atom.
[0057] In one aspect R.sup.4 and R.sup.5 are hydrogen or
C.sub.1-C.sub.4 alkyl. In another aspect R.sup.4 and R.sup.5
preferably each represent a hydrogen atom.
[0058] In another aspect of the invention R.sup.6 represents a
phenyl group optionally substituted by one or more (e.g. one, two,
three or four) substituents independently selected from halogen
(e.g. fluorine, chlorine, bromine or iodine), amino, nitro, cyano,
sulphonyl, sulphonamido, C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, alkyl, C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, haloalkoxy, methylenedioxy and C.sub.1-C.sub.6,
preferably C.sub.1-C.sub.4, alkylsulphonyl.
[0059] In another aspect of the invention R.sup.6 represents a
phenyl group optionally substituted by one or more (e.g. one, two,
three or four) substituents independently selected from halogen
(e.g. fluorine, chlorine, bromine or iodine), amino, nitro, cyano,
sulphonyl, sulphonamido, C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4 alkyl, C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4,
haloalkoxy and C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4,
alkylsulphonyl.
[0060] In a further aspect R.sup.1 is phenyl optionally substituted
by halogen or methylenedioxy. In a still further aspect R.sup.6 is
most preferably a phenyl group substituted by halogen. Examples of
R.sup.6 include 3-chlorophenyl, chlorophenyl or, especially,
3,4-dichlorophenyl.
[0061] R.sup.7 and R.sup.8 each independently represent a hydrogen
atom or a group selected from C.sub.1-C.sub.6, preferably
C.sub.1-C.sub.4, hydroxyalkyl C.sub.3-C.sub.6 cycloalkyl
(cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl) and
C.sub.1-C.sub.6, preferably C.sub.1-C.sub.4, alkyl optionally
substituted by phenyl (e.g. one or two phenyl groups).
[0062] Most preferably, R.sup.7 and R.sup.8 each independently
represent a hydrogen atom, or a group selected from C.sub.2
hydroxyalkyl, cyclopropyl and C.sub.1-C.sub.2 alkyl optionally
substituted by phenyl.
[0063] Compounds of the invention include all the Examples below,
some of which are:
[0064]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-methylbenzyl)amine,
[0065]
N-[4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)phenyl]ac-
etamide,
[0066]
3-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)phenol,
[0067]
N-[(4-Chloro-1-methyl-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl-
)-4-piperidinamine,
[0068]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-methyl-2-furyl)methy-
l]amine,
[0069]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-nitrobenzyl)amine,
[0070] N-Benzyl-1-(3,4-dichlorobenzyl)-4-piperidinamine,
[0071]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-fluorobenzyl)amine,
[0072]
N-(2,6-Dichlorobenzyl)-1-(3,4-dichlorobenzyl)-4-piperidinamine,
[0073] N,1 -Bis(3,4-dichlorobenzyl)-4-piperidinamine,
[0074]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(2-pyridinylmethyl)amine-
,
[0075]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(3-methyl-2-thienyl)met-
hyl]amine,
[0076]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-methyl-2-thienyl)met-
hyl]amine,
[0077]
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-methoxyph-
enol,
[0078]
4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-nitrophen-
ol,
[0079]
3({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-4H-chromen-4-
-one,
[0080]
N-((5-Chloro-1,3dimethyl-1H-pyrazol-4-yl)methyl]-1-(3,4-dichloroben-
zyl)-4-piperidinamine,
[0081]
N-[(4-Chloro-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-piper-
idinamine,
[0082]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl)-N-{[1-(4methylbenzyl)-1H-p-
yrazol-5-yl]methyl}amine,
[0083]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(2-phenyl-1H-imidazol-4-
-yl)methyl]amine,
[0084]
N-[(2-Chloro-3-quinolinyl)methyl]-1-(3,4-dichlorobenzyl)-4-piperidi-
namine,
[0085]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(6-methyl-2-pyridinyl)m-
ethyl]amine,
[0086]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-quinolinylmethyl)amin-
e,
[0087]
[5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-furyl]me-
thyl acetate,
[0088]
4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-1,5-dimethy-
l-2-phenyl-1,2-dihydro-3H-pyrazol-3-one,
[0089]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-pyridinylmethyl)amine-
,
[0090]
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-nitrophen-
ol,
[0091]
N-[2-(tert-Butylsulfanyl)benzyl]-1-(3,4-dichlorobenzyl)-4-piperidin-
amine,
[0092]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-ethylbenzyl)amine,
[0093]
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-hydroxybe-
nzoic acid,
[0094]
N-(1,3-Benzodioxol-4-ylmethyl)-1-(3,4-dichlorobenzyl)-4-piperidinam-
ine,
[0095]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(1,3-thiazol-2-ylmethyl)-
amine,
[0096]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-ethyl-2-furyl)methyl-
]amine,
[0097]
N-[1-(3,4-Dichlorobenzyl)piperidinyl]-N-(2-quinolinylmethyl)amine,
[0098]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-quinolinylmethyl)amin-
e,
[0099]
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-hydroxy-3-
-methoxybenzoic acid,
[0100]
N-[(4-Bromo-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-piperi-
dinamine,
[0101]
2-[2-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-6-methox-
yphenoxy]acetic acid,
[0102]
N-[(4-Bromo-1-methyl-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-
-4-piperidinamine,
[0103]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-iodobenzyl)amine,
[0104]
3-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-6,7-dimethy-
l-4H-chromen-4-one,
[0105]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-isopropoxybenzyl)amin-
e,
[0106]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-([1-methyl-1H-benzimidaz-
ol-2-yl)methyl]amine,
[0107]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-methylbenzyl)amine,
[0108]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-pyridinylmethyl)amine-
,
[0109]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(2,4-dimethylbenzyl)amin-
e,
[0110] Ethyl
5-({[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-met-
hyl-3-furoate,
[0111] N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-furamide,
[0112]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-[3-(4-pyridinyl)-1,2,4-o-
xadiazol-5-yl]butanamide,
[0113]
2-{[5-(1-Benzyl-2-oxo-1,2-dihydro-3-pyridinyl)-4-methyl-4H-1,2,4-tr-
iazol-3-yl]sulfanyl}-N-[1-(3,4-dichlorobenzyl)piperidinyl]propanamide,
[0114]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-6-methoxy-4-quinolinecarbo-
xamide
[0115]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(2-furyl)-4-quinolinecar-
boxamide,
[0116]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(2-methyl-1-oxo-1,2-dihy-
dro-3-isoquinolinyl)butanamide,
[0117]
3-(1,3-Benzothiazol-2-ylsulfanyl)-N-[1-(3,4-dichlorobenzyl)-4-piper-
idinyl]propanamide,
[0118]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(3,5-methoxyphenyl)aceta-
mide,
[0119]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(2-methoxyphenyl)acetami-
de,
[0120]
2-[5-Chloro-2-oxo-1,3-benzothiazol-3(2H)-yl]-N-[1-(3,4-dichlorobenz-
yl)-4-piperidinyl]acetamide,
[0121]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[(4,6-dimethyl-2-pyrimid-
inyl)sulfanyl]acetamide,
[0122]
2-(1-Benzothiophen-3-yl)-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]ac-
etamide,
[0123]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(3,4-dimethoxyphenyl)but-
anamide,
[0124]
5-Cyclohexyl-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]pentanamide,
[0125]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-fluoro-2-methylbenzamide-
,
[0126]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1-phenyleth-
yl)phthalamide,
[0127]
2-Cyclopentyl-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]acetamide,
[0128]
4-Chloro-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-nitrobenzamide,
[0129]
2,2-Dichloro-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-1-methylcyclo-
propanecarboxamide,
[0130] tert-Butyl
4-[5-({[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}carbo-
nyl)-2-methoxyphenyl]-1-piperazinecarboxylase,
[0131]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-5-oxo-1-(2-thienylmethyl)--
3-pyrrolidinecarboxamide,
[0132]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[2-oxo-1,3-benzoxazol-3(-
2H)-yl]propanamide,
[0133]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-fluorobenzamide,
[0134]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-methylbenzamide,
[0135]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-methylbenzamide,
[0136]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(hydroxymethyl)benzamide-
,
[0137]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-{2-[(methyls-
ufanyl)methyl]-4-pyrimidinyl}-1,2-ethanediamine,
[0138]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[2-(methylsu-
fanyl)-6-(trifluoromethyl)-4-pyrimidinyl]-1,2-ethanediamine,
[0139]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[5-methoxy-2-
-(methylsulfanyl)-4-pyrimidinyl]-1,2 ethanediamine,
[0140]
2-({4-[(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)amino]-
-2-pyrimidinyl}amino)-1-ethanol,
[0141]
N.sup.4-2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-6-met-
hyl-2,4-pyrimidinediamine,
[0142]
N.sup.4-(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-N.su-
p.2,6-dimethyl-2,4-pyrimidinediamine
[0143]
2-Chloro-N.sup.4-cyclopropyl-N.sup.6-(2-{[1-3,4-dichlorobenzyl)-4-p-
iperidinyl]amino}ethyl)-4,6-pyrimidinediamine,
[0144]
N.sup.1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(4-phenyl-2-pyr-
imidinyl)-1,2-ethanediamine,
[0145]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(trifluor-
omethyl)-2-pyrimidinyl]-1,2-ethanediamine,
[0146]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(propylsu-
lfanyl)-2-pyrimidinyl]-1,2-ethanediamine,
[0147]
N.sup.2-(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-N.su-
p.4,6-dimethyl-2,4-pyrimidinediamine,
[0148]
N.sup.4-Cyclopropyl-N.sup.2-(2-{[1-(3,4-chlorobenzyl)-4-piperidinyl-
]amino}ethyl)-2,4-pyrimidinediamine,
[0149]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(3-pyridi-
nyl)-2-pyrimidinyl]-1,2-ethanediamine,
[0150]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(3-thieny-
l)-2-pyrimidinyl]-1,2-ethanediamine,
[0151]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(2-thieny-
l)-2-pyrimidinyl]-1,2-ethanediamine,
[0152]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1-methyl-1H-
-pyrazolo[3,4-d]pyrimidin-4-yl)-1,2-ethanediamine,
[0153]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1H-purin-6--
yl)-1,2-ethanediamine,
[0154]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(5-methylthi-
eno[2,3-d]pyrimidin4-yl)-1,2-ethanediamine,
[0155]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(7-methylthi-
eno[3,2-d]pyrimidin4-yl)-1,2-ethanediamine,
[0156]
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(9-methyl-9H-
-purin-6-yl)-1,2-ethanediamine,
[0157]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-{[5-(trifluoromethyl)-2--
pyridinyl]sulfanyl}acetamide,
[0158]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(5-methyl-1-phenyl-1H-py-
razol-4-yl)acetamide,
[0159]
N-[1-(3,4-Dichlorobenzyl)piperidinyl]-5-oxo-5-phenylpentanamide,
[0160]
2-[2-(4-Chlorophenyl)-5-methyl-1,3-thiazol-4-yl]-N-[1-(3,4-dichloro-
benzyl)-4-piperidinyl]acetamide,
[0161]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(phenylsulfanyl)acetamid-
e,
[0162]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(4-fluorophenyl)acetamid-
e,
[0163]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[2-(2-pyrazinyl)-1,3-thi-
azol-4-yl]acetamide,
[0164]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[(5-phenyl-2-pyrimidinyl-
)sulfanyl]acetamide,
[0165]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1,2,4-o-
xadiazol-5-yl]propanamide,
[0166]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-1H-benzimidazol-2-amine,
[0167]
2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}-N-(3-methoxyphenyl)-
acetamide, dihydrochloride salt,
[0168]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N'-(3,4-dichlorophenyl)ure-
a,
[0169]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N'-(3-methoxyphenyl)urea,
and
[0170]
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-methoxybenzyl)amine,
dihydrochloride salt.
[0171] The present invention further provides a process for the
preparation of a compound of formula (I) or (Ia) which
comprises:
[0172] (a) when n is at least 1, the CR.sup.2R.sup.3 group attached
directly to T is CHR.sup.3 and T is NR.sup.10, reacting a compound
of formula 9
[0173] wherein n' is 0 or an integer from 1 to 3 and R.sup.1,
R.sup.2, R.sup.3, m and Q are as defined above, with a compound of
formula 10
[0174] or a salt thereof, wherein X.sup.1, X.sup.2, X.sup.3,
X.sup.4, Z, R.sup.6 and R.sup.10 are as defined above, in the
presence of a reducing agent; or
[0175] (b) when n is at least 1, the CR.sup.2R.sup.3 group attached
directly to T is C(C.sub.1-C.sub.4 alkyl).sub.2 and T is NR.sup.10,
reacting a compound of formula 11
[0176] wherein n' is 0 or an integer from 1 to 3, R.sup.2' and
R.sup.3' each independently represent a C.sub.1-C.sub.4 alkyl
group, and R.sup.1, R.sup.2, R.sup.3, R.sup.10, m and Q are as
defined above, with a compound of formula 12
[0177] wherein X.sup.1, X.sup.2, X.sup.3, X, Z and R.sup.6 are as
defined above, in the presence of a reducing agent; or
[0178] (c) when T is C(O)NR.sup.10, reacting a compound of formula
13
[0179] wherein R.sup.1, R.sup.2, R.sup.3, Q, m and n are as defined
above, with a compound of formula (III) or a salt thereof as
defined in (a) above; or
[0180] (d) when m is 1 and Q is NR.sup.9, reacting a compound of
formula (VII), R.sup.1-L.sup.1, wherein L.sup.1 represents a
leaving group (e.g. a halogen atom) and R.sup.1 is as defined
above, with a compound of formula 14
[0181] or a salt thereof, wherein, T, X.sup.1, X.sup.2, X.sup.3,
X.sup.4, Z, R.sup.2, R.sup.3, R.sup.6 and R.sup.9 are as defined
above; or
[0182] (e) when at least one of R.sup.4 and R.sup.5 represents a
hydrogen atom, reacting a compound of formula 15
[0183] or a salt thereof wherein R.sup.1, R.sup.2, R.sup.3, Q, m,
n, X.sup.1, X.sup.2, X.sup.3, X.sup.4 and T are as defined above,
with a compound of general formula (X), R.sup.6--C(O)--R.sup.20,
wherein R.sup.20 represents a hydrogen atom or a C.sub.1-C.sub.4
alkyl group and R.sup.6 is as defined above, in the presence of a
reducing agent; or
[0184] (f) reacting a compound of formula (IX) as defined in (e)
above, with a compound of formula 16
[0185] wherein L.sup.2 represents a leaving group (e.g. a halogen
atom) and Z and R.sup.6 are as defined above; or
[0186] (g) when T is NR.sup.10, reacting a compound of formula
R.sup.1-(Q).sub.m-(CR.sup.2R.sup.3).sub.n-L.sup.3 (XII)
[0187] wherein L.sup.3 represents a leaving group (e.g. a halogen
atom) and R.sup.1, R.sup.2, R.sup.3, n and Q are as defined above,
with a compound of formula (III) or a salt thereof as defined in
(a) above; or
[0188] (h) when T is NHC(O)NR.sup.10, reacting a compound of
formula
R.sup.1-(Q).sub.m-(CR.sup.2R.sup.3).sub.n--N.dbd.C.dbd.O (XIII)
[0189] wherein R.sup.1, R.sup.2, R.sup.3, Q, m and n are as defined
above, with a compound of formula (III) or a salt thereof as
defined in (a) above, or
[0190] (i) when T is C(O)NH, Z is CH.sub.2, n is 1, R.sup.2 and
R.sup.3 are hydrogen or C.sub.1-C.sub.4 alkyl and Q is oxygen or
sulphur, reacting a compound of formula (XIV): 17
[0191] wherein Hal is a suitable halogen (such as bromo or chloro),
R.sup.2, R.sup.3, X.sup.1, X.sup.2, X.sup.3, X.sup.4, Z and R.sup.6
are as defined above, with R.sup.1OH or R.sup.1SH in the presence
of a suitable base (such as potassium carbonate or sodium or
potassium hydroxide);
[0192] and optionally after (a), (b), (c), (d), (e), (f), (g), (h)
or (i) forming a pharmaceutically acceptable salt or solvate of the
compound of formula (I) or (Ia) obtained.
[0193] Compounds of formulae (II) to (XIV) are either commercially
available, or are known in the literature or may be prepared using
known techniques.
[0194] It will be appreciated by those skilled in the art that in
the processes of the present invention certain functional groups
such as hydroxyl or amino groups in the starting reagents or
intermediate compounds may need to be protected by protecting
groups. Thus, the preparation of the compounds of formula (I) or
(Ia) may involve, at an appropriate stage, the addition and
subsequent removal of one or more protecting groups.
[0195] The protection and deprotection of functional groups is
described in `Protective Groups in Organic Chemistry`, edited by J.
W. F. McOrnie, Plenum Press (1973) and `Protective Groups in
Organic Synthesis`, 2nd edition, T. W. Greene and P. G. M. Wuts,
Wiley-Interscience (1991).
[0196] The compounds of the invention and intermediates may be
isolated from their reaction mixtures, and if necessary further
purified, by using standard techniques.
[0197] The compounds of formula (I) and (Ia) have activity as
pharmaceuticals, in particular as modulators of chemokine receptor
activity. More particularly, the compounds have utility as
modulators of the activity of chemokine receptors CCR1 and/or
CCR3.
[0198] A further aspect of the invention involves the use of a
compound of formula (I) or (Ia) in the treatment of conditions or
diseases in which modulation of chemokine receptor activity is
beneficial.
[0199] Thus, compounds of formula (I) or (Ia) may be used in the
treatment of autoimmune, inflammatory, proliferative and
hyperproliferative diseases and immunologically-mediated diseases
including rejection of transplanted organs or tissues and Acquired
Immunodeficiency Syndrome (AIDS). Examples of these conditions
include:
[0200] (1) (the respiratory tract) obstructive airways diseases
including chronic obstructive pulmonary disease (COPD); asthma,
such as bronchial, allergic, intrinsic, extrinsic and dust asthma,
particularly chronic or inveterate asthma (e.g. late asthma and
airways hyper-responsiveness); bronchitis; acute, allergic,
atrophic rhinitis and chronic rhinitis including rhinitis caseosa,
hypertrophic rhinitis, rhinitis purulenta, rhinitis sicca and
rhinitis medicamentosa; membranous hinitis including croupous,
fibrinous and pseudomembranous rhinitis and scrofoulous rhinitis;
seasonal rhinitis including rhinitis nervosa (hay fever) and
vasomotor rhinitis; sarcoidosis, farmer's lung and related
diseases, fibroid lung and idiopathic interstitial pneumonia;
[0201] (2) (bone and joints) rheumatoid arthritis, osteoarthritis,
seronegative spondyloarthropathies (including ankylosing
spondylitis, psoriatic arthritis and Reiter's disease), Behcet's
disease, Sjogren's syndrome and systemic sclerosis;
[0202] (3) (skin) psoriasis, atopical dermatitis, contact
dermatitis and other eczmatous dermitides, seborrheetic dermatitis,
Lichen planus, Pemphigus, bullous Pemphigus, Epidermolysis bullosa,
urticaria, angiodermas, vasculitides, erythemas, cutaneous
eosinophilias, uveitis, Alopecia areata and vemal
conjunctivitis;
[0203] (4) (gastrointestinal tract) Coeliac disease, proctitis,
eosinopilic gastro-enteritis, mastocytosis, Crohn's disease,
inflammatory bowel disease, irritable bowel syndrome, ulcerative
colitis, food-related allergies which have effects remote from the
gut e.g., migraine, rhinitis and eczema;
[0204] (5) (other tissues and systemic disease) multiple sclerosis,
atherosclerosis, Acquired Immunodeficiency Syndrome (AIDS), lupus
erythematosus, systemic lupus, erytheinatosus, Hashimoto's
thyroiditis, myasthenia gravis, type I diabetes, nephrotic
syndrome, cosinophilia fascitis, hyper IgE syndrome, lepromatous
leprosy, sezary syndrome and idiopathic thrombocytopenia pupura;
and
[0205] (6) (allograft rejection) acute and chronic following, for
example, transplantation of kidney, heart, liver, lung, bone
marrow, skin and cornea; and chronic graft versus host disease
[0206] Thus, the present invention provides a compound of formula
(I) or (Ia), or a pharmaceutically acceptable salt thereof, a
solvate thereof or a solvate of a salt thereof; as hereinbefore
defined for use in therapy.
[0207] In a further aspect, the present invention provides the use
of a compound of formula (I) or (Ia), or a pharmaceutically
acceptable salt thereof, a solvate thereof or a solvate of a salt
thereof; as hereinbefore defined in the manufacture of a medicament
for use in therapy.
[0208] In the context of the present specification, the term
"therapy" also includes "prophylaxis" unless there are specific
indications to the contrary. The terms "therapeutic" and
"therapeutically" should be construed accordingly.
[0209] Prophylaxis is expected to be particularly relevant to the
treatment of persons who have suffered a previous episode of, or
are otherwise considered to be at increased risk of, the disease or
condition in question. Persons at risk of developing a particular
disease or condition generally include those having a family
history of the disease or condition, or those who have been
identified by genetic testing or screening to be particularly
susceptible to developing the disease or condition.
[0210] In another aspect the present invention provides the use of
a compound of formula (I), wherein Z is CR.sup.4R.sup.5, C(O) or
CR.sup.4R.sup.5-Z.sup.1; Z.sup.1 is C.sub.1-4 alkylene, C.sub.2-4
alkenylene or C(O)NH; R.sup.1 represents a C.sub.1-C.sub.12 alkyl
group optionally substituted by one or more substituents
independently selected from cyano, hydroxyl, C.sub.1-C.sub.6
alkoxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-7 cycloalkyl,
C.sub.1-C.sub.6 alkoxycarbonyl and phenyl (itself optionally
substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents C.sub.2-C.sub.6 alkenyl optionally substituted
by phenyl (itself optionally substituted by one or more of halogen,
nitro, cyano, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or
R.sup.1 represents a 3- to 14-membered saturated or unsaturated
ring system which optionally comprises up to two ring carbon atoms
that form carbonyl groups and which optionally further comprises up
to 4 ring heteroatoms independently selected from nitrogen, oxygen
and sulphur, wherein the ring system is optionally substituted by
one or more substituents independently selected from: halogen,
cyano, nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.- 1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.s- ub.6 alkyl),
aryl(C.sub.1-C.sub.6 alkyl)S(O).sub.2, hetercyclyl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
hetercyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
halolylcarbonylamino, SO.sub.3H, --NR.sup.7R.sup.8,
--C(O)NR.sup.23R.sup.24, S(O).sub.2NR.sup.18R.sup.19,
S(OR).sub.2R.sup.20, R.sup.25C(O), carboxyl C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; wherein the foregoing aryl
and heterocyclyl moieties are optionally substituted by one or more
of halogen, oxo, hydroxy, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl),
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 haloalkoxy,
S(O).sub.2(C.sub.1-C.sub.6 alkyl), C(O)NH.sub.2, carboxy or
C.sub.1-C.sub.6 alkoxycarbonyl; m is 0 or 1; Q represents an oxygen
or sulphur atom or a group NR.sup.9, C(O), C(O)NR.sup.9,
NR.sup.9C(O) or CH.dbd.CH; n is 0, 1, 2, 3, 4, 5 or 6 provided that
when n is 0, then m is 0; each R.sup.2 and R.sup.3 independently
represents a hydrogen atom or a C.sub.1-C.sub.4 alkyl group, or
(CR.sup.2R.sup.3).sub.n represents C.sub.3-C.sub.7 cycloalkyl
optionally substituted by C.sub.1-C.sub.4 alkyl; T represents a
group NR.sup.10, C(O)NR.sup.10, NR.sup.11C(O)NR.sup.10 or
C(O)NR.sup.10NR.sup.11; X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are,
independently, CH.sub.2, CHR.sup.12 (wherein each R.sup.12 is,
independently, C.sub.1-C.sub.4 alkyl or C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl)) or C.dbd.O; or, when they are
CHR.sup.12, the R.sup.12 groups of X.sub.1 and X.sup.3 or X.sup.4,
or, X.sup.2 and X.sup.3 or X.sup.4 join to form a two or three atom
chain which is CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2,
CH.sub.2OCH.sub.2 or CH.sub.2SCH.sub.2; provided always that at
least two of X.sup.1, X.sup.2, X.sup.3 and X.sup.4 are CH.sub.2;
R.sup.4 and R.sup.5 each independently represent a hydrogen atom or
a C.sub.1-C.sub.4 alkyl group; R.sup.6 is aryl or heterocyclyl,
both optionally substituted by one or more of: halogen, cyano,
nitro, oxo, hydroxyl, C.sub.1-C.sub.8 alkyl, C.sub.1-C.sub.6
hydroxyalkyl, C.sub.1-C.sub.6 haloalkyl, C.sub.1-C.sub.6
alkoxy(C.sub.1-C.sub.6 alkyl), C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylthio(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylcarbonyloxy(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6
alkylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl), heterocyclyl(C.sub.1-C.sub.6 alkyl),
arylS(O).sub.2(C.sub.1-C.sub.6 alkyl),
heterocyclylS(O).sub.2(C.sub.1-C.sub.6 alkyl), aryl(C.sub.1-C.sub.6
alkyl)S(O).sub.2, heterocyclyl(C.sub.1-C.sub.6 alkyl)S(O).sub.2,
C.sub.2-C.sub.6 alkenyl, C.sub.1-C.sub.6 alkoxy,
carboxy-substituted C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6
haloalkoxy, C.sub.1-C.sub.6 hydroxyalkoxy, C.sub.1-C.sub.6
alkylcarboxy-substituted C.sub.1-C.sub.6 alkoxy, aryloxy,
heterocyclyloxy, C.sub.1-C.sub.6 alkylthio, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.6 alkylthio), C.sub.3-C.sub.6 alkynylthio,
C.sub.1-C.sub.6 alkylcarbonylamino, C.sub.1-C.sub.6
haloalkylcarbonylamino, SO.sub.3H, --NR.sup.16R.sup.17,
--C(O)NR.sup.21R.sup.22, S(O).sub.2NR.sup.13R.sup.14,
S(O).sub.2R.sup.15, R.sup.26C(O), aroxyl, C.sub.1-C.sub.6
alkoxycarbonyl, aryl and heterocyclyl; is wherein the foregoing
aryl and heterocyclyl moieties are optionally substituted by one or
more of halogen, nitro, cyano, C.sub.1-C.sub.6 alkyl,
C.sub.1-C.sub.6 haloalkyl, phenyl(C.sub.1-C.sub.6 alkyl),
C.sub.1-C.sub.6 alkoxy, C.sub.1-C.sub.6 haloalkoxy,
S(O).sub.2(C.sub.1-C.sub.6 alkyl), C(O)NH.sub.2, carboxy or
C.sub.1-C.sub.6 alkoxycarbonyl; R.sup.7, R.sup.8, R.sup.9,
R.sup.10, R.sup.11, R.sup.13, R.sup.14, R.sup.16, R.sup.17,
R.sup.18, R.sup.19, R.sup.21, R.sup.22, R.sup.23 and R.sup.24 are,
independently hydrogen, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6
haloalkyl, C.sub.1-C.sub.6 hydroxyalkyl, C.sub.3-C.sub.7
cycloalkyl, C.sub.3-C.sub.7 Cycloalkyl(C.sub.1-C.sub.4 alkyl) or
phenyl(C.sub.1-C.sub.6 alkyl); R.sup.15 and R.sup.20 are,
independently, C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 hydroxyalkyl,
C.sub.3-C.sub.6 cycloalkyl, C.sub.3-C.sub.7
cycloalkyl(C.sub.1-C.sub.4 alkyl) or C.sub.1-C.sub.6 alkyl
optionally substituted by phenyl; and, R.sup.25 and R.sup.26 are,
independently, C.sub.1-C.sub.6 alkyl or phenyl (optionally
substituted by one or more of halogen, nitro, cyano,
C.sub.1-C.sub.6 alkyl, C.sub.1-C.sub.6 haloalkyl,
phenyl(C.sub.1-C.sub.6 alkyl), C.sub.1-C.sub.6 alkoxy,
C.sub.1-C.sub.6 haloalkoxy, S(O).sub.2(C.sub.1-C.sub.6 alkyl),
C(O)NH.sub.2, carboxy or C.sub.1-C.sub.6 alkoxycarbonyl); or a
pharmaceutically acceptable salt thereof or solvate thereof or a
solvate of a salt thereof, in the manufacture of a medicament for
the modulation of a chemokine receptor (such as CCR1 or CCR3). In a
further aspect such medicament is for the treatment of asthma.
[0211] The invention also provides a method of treating an
inflammatory disease in a person suffering from, or at risk of,
said disease, which comprises administering to the person a
therapeutically effective amount of a compound of formula (I) or
(Ia), or a pharmaceutically acceptable salt thereof a solvate
thereof or a solvate of a salt thereof, as hereinbefore
defined.
[0212] For the above-mentioned therapeutic uses the dosage
administered will, of course, vary with the compound employed, the
mode of administration, the treatment desired and the disorder
indicated.
[0213] A compound of formula (I) or (Ia) or a pharmaceutically
acceptable salt, solvate or solvate of a salt, may be used on its
own but will generally be administered in the form of a
pharmaceutical composition in which the formula (I) or (Ia)
compound, salt, solvate or solvate of salt (active ingredient) is
in association with a pharmaceutically acceptable adjuvant, diluent
or carrier. Depending on the mode of administration, the
pharmaceutical composition will preferably comprise from 0.05 to
99% w (per cent by weight), more preferably from 0.05 to 80% w,
still more preferably from 0.10 to 70% w, and even more preferably
from 0.10 to 50% w, of active ingredient, all percentages by weight
being based on total composition.
[0214] The present invention also provides a pharmaceutical
composition comprising a compound of formula (I) or (Ia), or a
pharmaceutically acceptable salt, solvate or solvate of salt
thereof as hereinbefore defined, in association with a
pharmaceutically acceptable adjuvant, diluent or carrier.
[0215] The invention further provides a process for the preparation
of a pharmaceutical composition of the invention which comprises
mixing a compound of formula (I) or (Ia), or a pharmaceutically
acceptable salt, solvate or solvate of salt thereof, as
hereinbefore defined, with a pharmaceutically acceptable adjuvant,
diluent or carrier.
[0216] The pharmaceutical compositions may be administered
topically (e.g. to the lung and/or airways or to the skin) in the
form of solutions, suspensions, heptafluoroalkane aerosols and dry
powder formulations; or systemically, e.g. by oral administration
in the form of tablets, capsules, syrups, powders, aerosols or
granules, or by parenteral administration in the form of solutions
or suspensions, or by subcutaneous administration or by rectal
administration in the form of suppositories or transdermally.
[0217] The present invention will be further explained by reference
to she following illustrative examples.
EXAMPLES 1-47
(i) tert-Butyl 1-(3,4-dichlorobenzyl)-4-piperdinylcarbamate
[0218] 18
[0219] Sodium triacetoxyborobydride (6 g) was added to a stir
solution of 3,4-dichlorobenzaldehyde (4.2 g) and 1,1-dimethylethyl
4-piperidinyl carbamate (4 g) in dichloromethane (50 ml). The
mixture was stirred at room temperature for 4 h then partitioned
between ethyl acetate and aqueous sodium hydrogencarbonate. The
organic layer was washed with water, dried and evaporated under
reduced pressure. The residue was triturated with ether to give a
white solid (3.5 g). Used directly.
(ii) 1-(3,4-Dichlorobenzyl)-4-piperidinamine, di-trifluoroacetate
salt
[0220] 19
[0221] The product from step (i) (3.5 g) was treated with
trifluoroacetic acid (10 ml) in dichloromethane (40 ml). After 72
h, the solution was evaporated, the residue triturated with ether
and the solid (4.3 g) collected.
[0222] MS: APCI(+ve) 259/61 (M+1)
(iii) EXAMPLES 1-47
[0223] The product from step (ii) (2 mg), the appropriate aldehyde
(2 equivalents), sodium triacetoxyborohydride (3 equivalents) and
diisopropylethylamine (2 equivalents) in acetonitrile (0.08 ml) and
1-methyl-2-pyrrolidinone (0.12 ml) was left at room temperature for
24 h. The reaction mixture was evaporated to dryness and the
residue dissolved in dimethylsuphoxide (0.4 ml).
EXAMPLE 1
N-[1-(3,4-Dichlorebenzyl)-4-piperidinyl]-N-(4-methylbenzyl)amine
[0224] 20
[0225] MS: AP.CI(+ve) 363 (M+1)
EXAMPLE 2
N-[4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)phenyl]acetamide
[0226] 21
[0227] MS: APCI(+ve) 406 (M+1)
EXAMPLE 3
3-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)phenol
[0228] 22
[0229] MS: APCI(+ve) 365 (M+1)
EXAMPLE 4
N-[(4-Chloro-1-methyl-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-pip-
eridinamine
[0230] 23
[0231] MS: APCI(+ve) 389 (M+1)
EXAMPLE 5
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-methyl-2-furyl)methyl]amine
[0232] 24
[0233] MS: APCI(+ve) 353 (M+1)
EXAMPLE 6
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl-N-(4-nitrobenzyl)amine
[0234] 25
[0235] MS: APCI(+ve) 394 (M+1)
EXAMPLE 7
N-Benzyl-1-(3,4-dichlorobenzyl)-4-piperidinamine
[0236] 26
[0237] MS: APCI(+ve) 349 (M+1)
EXAMPLE 8
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-fluorobenzyl)amine
[0238] 27
[0239] MS: APCI(+ve) 367 (M+1)
EXAMPLE 9
N-(2,6-Dichlorobenzyl)-1-(3,4-dichlorobenzyl)-4-piperidinamine
[0240] 28
[0241] MS: APCI(+ve) 419 (M+1)
EXAMPLE 10
N,1-Bis(3,4-chlorobenzyl)-4-piperidinamine
[0242] 29
[0243] MS: APCI(+ve) 419 (M+1)
EXAMPLE 11
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(2-pyridinylmethyl)amine
[0244] 30
[0245] MS: APCI(+ve) 350 (M+1)
EXAMPLE 12
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(3-methyl-2-thienyl)methyl}ami-
ne
[0246] 31
[0247] MS: APCI(+ve) 369 (M+1)
EXAMPLE 13
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-methyl-2-thienyl)methyl]ami-
ne
[0248] 32
[0249] MS: APCI(+ve) 369 (M+1)
EXAMPLE 14
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-methoxyphenol
[0250] 33
[0251] MS: APCI(+ve) 395 (M+1)
EXAMPLE 15
4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-nitrophenol
[0252] 34
[0253] MS: APCI(+ve) 410 (M+1)
EXAMPLE 16
3-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-4H-chromen-4-one
[0254] 35
[0255] MS: APCI(+ve) 417 (M+1)
EXAMPLE 17
N-[(5Chloro-1,3-dimethyl-1H-pyrazol-4-yl)methyl]-1-(3,4-dichlorobenzyl)-4--
piperidinamine
[0256] 36
[0257] MS: APCI(+ve) 403 (M+1)
EXAMPLE 18
N-[(4-Chloro-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-piperidinami-
ne
[0258] 37
[0259] MS: APCI(+ve) 373 (M+1)
EXAMPLE 19
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-{[1-(4-methylbenzyl)-1H-pyrazol-
-5-yl]methyl}amine
[0260] 38
[0261] MS: APCI(+ve) 443 (M+1)
EXAMPLE 20
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(2-phenyl-1H-imidazol-4-yl)met-
hyl]amine
[0262] 39
[0263] MS: APCI(+vc) 414 (M+1)
EXAMPLE 21
N-[(2-Chloro-3-quinolinyl)methyl]-1-(3,4-dichlorobenzyl)-4-piperidinamine
[0264] 40
[0265] MS: APCI(+ve) 434 (M+1)
EXAMPLE 22
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(6-methyl-2-pyridinyl)methyl]a-
mine
[0266] 41
[0267] MS: APCI(+ve) 364 (M+1)
EXAMPLE 23
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-quinolinylmethyl)amine
[0268] 42
[0269] MS: APCI(+ve) 400 (M+1)
EXAMPLE 24
[5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-furyl]methyl
acetate
[0270] 43
[0271] MS: APCI(+ve) 411 (M+1)
EXAMPLE 25
4-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-1,5-dimethyl-2-phe-
nyl-1,2-dihydro-3H-pyrazol-3-one
[0272] 44
[0273] MS: APCI(+ve) 459 (M+1)
EXAMPLE 26
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-Pyridinylmethyl)amine
[0274] 45
[0275] MS: APCI(+ve) 350 (M+1)
EXAMPLE 27
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-mitrophenol
[0276] 46
[0277] MS:APCI(+ve) 410(M+1)
EXAMPLE 28
N-[2-(tert-Butylsulfanyl)benzyl]-1-(3,4-dichlorobenzyl)-4-piperidinamine
[0278] 47
[0279] MS: APCI(+ve) 437 (M+1)
EXAMPLE 29
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-ethylbenzyl)amine
[0280] 48
[0281] MS: APCI(+ve) 377 (M+1)
EXAMPLE 30
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl-2-hydroxybenzoic
acid
[0282] 49
[0283] MS: APCI(+ve) 409 (M+1)
EXAMPLE 31
N-(1,3-Benzodioxol-4-ylmethyl)-1-(3,4-dichlorobenzyl)-4-piperidiamine
[0284] 50
[0285] MS: APCI(+ve) 393 (M+1)
EXAMPLE 32
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(1,3-thiazol-2-ylmethyl)amine
[0286] 51
[0287] MS: APCI(+ve) 356 (M+1)
EXAMPLE 33
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(5-ethyl-2-furyl)methyl]amine
[0288] 52
[0289] MS: APCI(+ve) 367 (M+1)
EXAMPLE 34
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(2-quinolinylmethyl)amine
[0290] 53
[0291] MS: APCI(+ve) 400 (M+1)
EXAMPLE 35
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-quinolinylmethyl)amine
[0292] 54
[0293] MS: APCI(+ve) 400 (M+1)
EXAMPLE 36
5-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-2-hydroxy-3-methox-
ybenzoic acid
[0294] 55
[0295] MS: APCI(+ve) 439 (M+1)
EXAMPLE 37
N-[(4-Bromo-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-piperidinamin-
e
[0296] 56
[0297] MS: APCI(+ve) 419 (M+1)
EXAMPLE 38
2-[2-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-6-methoxyphenox-
y]acetic acid
[0298] 57
[0299] MS: APCI(+ve) 453 (M+1)
EXAMPLE 39
N-[(4-Bromo-1-methyl-1H-pyrazol-3-yl)methyl]-1-(3,4-dichlorobenzyl)-4-pipe-
ridinamine
[0300] 58
[0301] MS: APCI(+ve) 433 (M+1)
EXAMPLE 40
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-iodobenzyl)amine
[0302] 59
[0303] MS: APCI(+ve) 475 (M+1)
EXAMPLE 41
3-({[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}methyl)-6,7-dimethyl-4H-ch-
romen-4-one
[0304] 60
[0305] MS: APCI(+ve) 445 (M+1)
EXAMPLE 42
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-isopropoxybenzyl)amine
[0306] 61
[0307] MS: APCI(+ve) 407 (M+1)
EXAMPLE 43
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-[(1-methyl-1H-benzimadizol-2-yl-
)methyl]amine
[0308] 62
[0309] MS: APCI(+ve) 403 (M+1)
EXAMPLE 44
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-methylbenzyl)amine
[0310] 63
[0311] MS: APCI(+ve) 363 (M+1)
EXAMPLE 45
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(3-pyridinylmethyl)amine
[0312] 64
[0313] MS: APCI(+ve) 350 (M+1)
EXAMPLE 46
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(2,4-dimethylbenzyl)amine
[0314] 65
[0315] MS: APCI(+vc) 377 (M+1)
EXAMPLE 47
Ethyl
5-({[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}methyl-2-methyl-3-fu-
roate
[0316] 66
[0317] MS: APCI(+ve) 425 (M+1)
EXAMPLES 48-73
(i) EXAMPLES 48-73
[0318] Bromo-tris-pyrrolidino-phosphonium hexafluorophosphate (2
equiv) was added to a solution of the product from Example 1 step
(ii) (hydrochloride salt) (1 mg), the appropriate acid (2
equivalents) and diisopropylethylamine (5 equivalents) in
dimethylformamide (0.17 ml) and was left at room temperature for 24
h. The reaction mixture was evaporated to dryness and the residue
dissolved in dimethylsulphoxide (0.3 ml).
EXAMPLE 48
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-furamide
[0319] 67
[0320] MS: APCI(+ve) 353 (M+1)
EXAMPLE 49
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-[3-(4-pyridinyl)-1,2,4-oxadiazo-
l-5-yl]butanamide
[0321] 68
[0322] MS: APCI(+ve) 474 (M+1)
EXAMPLE 50
2-{[5-(1-Benzyl-2-oxo-1,2-dihydro-3-pyridinyl)-4-methyl-4H-1,2,4-triazol-3-
-yl]sulfanyl}-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]propanamide
[0323] 69
[0324] MS: APCI(+ve) 611 (M+1)
EXAMPLE 51
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-6-methoxy-4-quinolinecarboxamide
[0325] 70
[0326] MS: APCI(+ve) 444 (M+1)
EXAMPLE 52
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(2-furyl)-4-quinolinecarboxamid-
e
[0327] 71
[0328] MS: APCI(+ve) 480 (M+1)
EXAMPLE 53
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(2-methyl-1-oxo-1,2-dihydro-3-i-
soquinolinyl)butanamide
[0329] 72
[0330] MS: APCI(+ve) 486 (M+1)
EXAMPLE 54
3-(1,3-Benzothiazol-2-ylsulfanyl)-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-
propanamide
[0331] 73
[0332] MS: APCI(+ve) 480 (M+1)
EXAMPLE 55
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(3,5-dimethoxyphenyl)acetamide
[0333] 74
[0334] MS: APCI(+ve) 437 (M+1)
EXAMPLE 56
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(2-methoxyphenyl)acetamide
[0335] 75
[0336] MS: APCI(+ve) 407 (M+1)
EXAMPLE 57
2-[5-Chloro-2-ozo-1,3-benzothiazol-3(2H)-yl]-N-[1-(3,4-dichlorobenzyl)-4-p-
iperidinyl]acetamide
[0337] 76
[0338] MS: APCI(+ve) 486 (M+1)
EXAMPLE 58
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[(4,6-dimethyl-2-pyrimidinyl)su-
lfanyl]acetamide
[0339] 77
[0340] MS: ACI(+ve) 439 (M+1)
EXAMPLE 59
2-(1-Benzothiophen-3-yl)-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]acetamide
[0341] 78
[0342] MS: APCI(+ve) 433 (M+1)
EXAMPLE 60
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(3,4-dimethoxyphenyl)butanamide
[0343] 79
[0344] MS: APCI(+vc) 465 (M+1)
EXAMPLE 61
5-Cyclohexyl-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]pentanamide
[0345] 80
[0346] MS: APCI(+ve) 425 (M+1)
EXAMPLE 62
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-fluoro-2-methylbenzamide
[0347] 81
[0348] MS: APCI(+vc) 395 (M+1)
EXAMPLE 63
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1-phenylethyl)phth-
alamide
[0349] 82
[0350] MS: APCI(+ve) 510 (M+1)
EXAMPLE 64
2-Cyclopentyl-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]acetamide
[0351] 83
[0352] MS: APCI(+ve) 369 (M+1)
EXAMPLE 65
4-Chloro-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-nitrobenzamide
[0353] 84
[0354] MS: APCI(+ve) 444 (M+1)
EXAMPLE 66
2,2-Dichloro-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-1-methylcyclopropane-
carboxamide
[0355] 85
[0356] MS: APCI(+ve) 411 (M+1)
EXAMPLE 67
tert-Butyl
4-[5-({[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}carbonyl)-2--
methoxyphenyl]-1-piperazinecarboxylate
[0357] 86
[0358] MS: APCI(+ve) 577 (M+1)
EXAMPLE 68
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-5-oxo-1-(2-thienylmethyl)-3-pyrro-
lidinecarboxamide
[0359] 87
[0360] MS: APCI(+ve) 466 (M+1)
EXAMPLE 69
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[2-oxo-1,3-benzoxazol-3(2H)-yl]-
propanamide
[0361] 88
[0362] MS: APCI(+ve) 448 (M+1)
EXAMPLE 70
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-fluorobenzamide
[0363] 89
[0364] MS: APCI(+ve) 381 (M+1)
EXAMPLE 71
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-methylbenzamide
[0365] 90
[0366] MS: APCI(+ve) 377 (M+1)
EXAMPLE 72
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-methylbenzamide
[0367] 91
[0368] MS: APCI(+ve) 377 (M+1)
EXAMPLE 73
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-4-(hydroxymethyl)benzamide
[0369] 92
[0370] MS: APCI(+ve) 393 (M+1)
EXAMPLES 74-93
(i) 1-(3,4-Dichlorobenzyl)-4-piperidinone
[0371] 93
[0372] A solution of 3,4-dichlorobenzyl chloride (2.8 ml),
4-ketopiperidine hydrochloride monohydrate and triethylamine (8 ml)
in dimethylformamide (30 ml) was stirred at room temperature for 20
h. The mixture was partitioned between water and ethyl acetate, the
organic layer dried and evaporated under reduced pressure.
Purification was by chromatography eluting with 40-50% ethyl
acetate/isohexane. Yield 2.1 g. MS: APCI(+ve) 258/60 (M+1)
(ii) tert-Butyl
2-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}ethylcarbam- ate
[0373] 94
[0374] A solution of the product from step (i) (1.61 g),
N-(tert-butoxycarbonyl)-ethylenediamine (1 g) and sodium
triacetoxyborohydride (2.12 g) in dichloromethane (20 ml) was
stirred at room temperature for 3 h. The mixture was partitioned
between water and ethyl acetate, the organic layer dried and
evaporated under reduced pressure. Yield 1.28 g. MS: APCI(+ve)
402/4 (M+1)
(ii) N-1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-1,2-ethanediamine,
tritrifluoroacetate salt
[0375] 95
[0376] The product from step (ii) (128 g) was treated with
trifluoroacetic acid (5 ml) in dichloromethane (10 ml). After 20 h,
the solution was evaporated, the residue triturated with ether and
the solid (1.62 g) collected.
[0377] MS: APCI(+ve) 302/4 (M+1)
(iv) EXAMPLES 74-93
[0378] The product from step (iii) (0.0026 g), the appropriate
activated halo-aromatic (1.25 equivalents) and
diisopropylethylamine (10 equivalents) in 1-methyl-2-pyrrolidinone
(0.15 ml) was heated at 100.degree. C. for 20 h The reaction
mixture was evaporated to dryness u and the residue dissolved in
dimethylsuphoxide (0.4 ml).
EXAMPLE 74
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(2-[(methylsulfanyl-
)methyl]-4-pyrimidinyl}-1,2-ethanediamine
[0379] 96
[0380] MS: APCI(+ve) 440(M+1)
EXAMPLE 75
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[2-(methylsulfanyl)-
-6-(trifluoromethyl)-4-pyrimidinyl]-1,2-ethanediamine
[0381] 97
[0382] MS: APCI(+ve) 494(M+1)
EXAMPLE 76
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[5-methoxy-2-(methy-
lsulfanyl)-4-pyrimidinyl]-1,2-ethanediamine
[0383] 98
[0384] MS: APCI(+ve) 456(M+1)
EXAMPLE 77
2-({4-[(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)amino]-2-pyri-
midinyl}amino)-1-ethanol
[0385] 99
[0386] MS: APCI(+ve) 439(M+1)
EXAMPLE 78
N.sup.4-(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-6-methyl-2,-
4-pyrimidinediamine
[0387] 100
[0388] MS: APCI(+ve) 409(M+1)
EXAMPLE 79
N.sup.4-(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-N.sup.2,6-d-
imethyl-2,4-pyrimidinediamine
[0389] 101
[0390] MS: APCI(+ve) 423(M+1)
EXAMPLE 80
2-Chloro-N.sup.4-cyclopropyl-N.sup.6-(2-{[1-(3,4-dichlorobenzyl)-4-piperid-
inyl]amino}ethyl)-4,6-pyrimidinediamine
[0391] 102
[0392] MS: APCI(+ve) 471(M+1)
EXAMPLE 81
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(4-phenyl-2-pyrimid-
inyl)-1,2-ethanediamine
[0393] 103
[0394] MS: APCI(+vc) 456(M+1)
EXAMPLE 82
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4(trifluoromethyl)-
-2-pyrimidinyl]-1,2-ethanediamine
[0395] 104
[0396] MS: APCI(+ve) 448(M+1)
EXAMPLE 83
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(propylsulfanyl)-
-2-pyrimidinyl]-1,2-ethanediamine
[0397] 105
[0398] MS: APCI(+ve) 454(M+1)
EXAMPLE 84
N.sup.2-(2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}ethyl)-N.sup.4,6-d-
imethyl-2,4-pyrimidinediamine
[0399] 106
[0400] MS: APCI(+ve) 423(M+1)
EXAMPLE 85
N.sup.4-Cyclopropyl-N.sup.2(2-{[-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-
ethyl)-2,4-pyrimidinediamine
[0401] 107
[0402] MS: APCI(+ve) 435(M+1)
EXAMPLE 86
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(3-pyridinyl)-2--
pyrimidinyl]-1,2-ethanediamine
[0403] 108
[0404] MS: APCI(+ve) 457(M+1)
EXAMPLE 87
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4-(3-thienyl)-2-py-
rimidinyl]-1,2-ethanediamine
[0405] 109
[0406] MS: APCI(+ve) 462(M+1)
EXAMPLE 88
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-[4(2-thienyl)-2-pyr-
imidinyl]-1,2-ethanediamine
[0407] 110
[0408] MS: APCI(+ve) 462(M+1)
EXAMPLE 89
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1-methyl-1H-pyrazo-
lo[3,4-d]pyrimidin-4-yl)-1,2-ethanediamine
[0409] 111
[0410] MS: APCI(+ve) 434(M+1)
EXAMPLE 90
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(1H-purin-6-yl)-1,2-
-ethanediamine
[0411] 112
[0412] MS: APCI(+ve) 420(M+1)
EXAMPLE 91
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(5methylthieno[2,3--
d]pyrimidin-4-yl)-1,2-ethanediamine
[0413] 113
[0414] MS: APCI(+ve) 450(M+1)
EXAMPLE 92
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(7-methylthieno[3,2-
d]pyrimidin-4-yl)-1,2-ethanediamine
[0415] 114
[0416] MS: APCI(+ve) 450(M+1)
EXAMPLE 93
N.sup.1-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N.sup.2-(9-methyl-9H-purin--
6-yl)-1,2-ethanediamine
[0417] 115
[0418] MS: APCI(+ve) 434(M+1)
EXAMPLE 94
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-{[5-(trifluoromethyl)-2-pyridin-
yl]sulfanyl}acetamide
[0419] 116
[0420] Carbonyldiimidazole (0.105 g) was added to a stirred
solution of 2-{[5-(trifluoromethyl)-2-pyridinyl]sulfanyl) acetic
acid (0.166 g) in dimethylformamide (2 ml). After 1 h a solution of
Is the product from Example 1 step (ii) (0.3 g) in a solution of
dimethylformamide and diisopropylethylamine (2 equivalents) (1.5
ml) was added and stirred at room temperature for 2 h The mixture
was partitioned between water and ethyl acetate, the organic layer
washed with water, dried and evaporated under reduced pressure. The
residue was triturated with ether and collected Yield 0.084 g as a
solid.
[0421] MS: APCI(+ve) 478/80 (M+1)
[0422] .sup.1H NMR: .delta. (DMSO-d6) 8.76(s, 1H), 8.11 (d, 1H),
8.02(dd, 1H), 7.59-7.53(m, 3H), 7.29(dd, 1H), 3.91(s, 1H),
3.58-3.45(m, 1H), 3.44(s, 2H), 2.70(br d, 2H), 2.03(br t, 2H),
1.70(br d, 2H), 1.46-1.37(m, 2H).
[0423] MP: 98.degree. C.
EXAMPLE 95
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl)-2-(5-methyl-1-phenyl-1H-pyrazol-4-
-yl)acetamide
[0424] 117
[0425] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and of
2-(5-methyl-1-phenyl-1H-pyrazol-4-yl)acetic acid (0.151 g) using
the method of Example 94.
[0426] Yield 0.18 g as a solid.
[0427] MS: APCI(+ve) 457/9 (M+1)
[0428] .sup.1H NMR: .delta. (DMSO-d6) 7.90(d, 1H), 7.59-7.38(m,
8H), 7.29(dd, 1H), 3.54-3.50(m, 1H), 3.45(s, 2H), 3.24(s, 2H),
2.72(br d, 2H), 224(s, 3H), 2.03(br t, 2H), 1.72(br d, 2H),
1.46-1.37(m, 2H).
[0429] MP: 165.degree. C.
EXAMPLE 96
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-5-oxo-5-phenylpentanamide
[0430] 118
[0431] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and of 5-oxo-5-phenylpentanoic acid (0.134 g)
using the method of Example 94. Yield 0.149 g as a solid.
[0432] MS: APCI(+ve) 433/5 (M+1)
[0433] .sup.1H NMR: .delta. (DMSO-d6) 7.96-7.93(m, 2H), 7.72(d,
1H), 7.65-7.50(m, 5H), 7.28(dd, 1H), 3.57-3.48(m, 1H), 3.44(s, 2H),
3.01(t, 2H), 2.72-2.67(m, 2H), 2.13(t, 2H), 2.04-1.98(m, 2H),
1.86-1.79(m, 2H), 1.69(br s, 2H), 1.41-1.32(m, 2H).
[0434] MP: 130.degree. C.
EXAMPLE 97
2-[2-(4-Chlorophenyl)-5-methyl-1,3-thizol-4-yl]-N-[1-(3,4-dichlorobenzyl)--
4-piperidinyl]acetamide
[0435] 119
[0436] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and 2-[2-(4-chlorophenyl)-5-methyl-1,3-thiazol
4-yl]acetic acid (0.187 g) using the method of Example 94. Yield
0.1 g as a solid.
[0437] MS: APCI(+ve) 510/2 (M+1)
[0438] 1H NMR: .delta. (DMSO-d6) 8.00(d, 1H), 7.85-7.82(m, 2H),
7.59-7.52(m, 4H), 7.29(dd, 1H), 3.57-3.51(m, 3H), 3.44(s, 2H),
2.72(br d, 2H), 2.41(s, 3H), 2.06(t, 2H), 1.73(br d, 2H),
1.48-1.38(m, 2H).
[0439] MP: 170.degree. C.
EXAMPLE 98
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(phenylsulfanyl)acetamide
[0440] 120
[0441] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and 2-(phenylsulfanyl)acetic acid (0.118 g)
using the method of Example 94. Yield 0.056 g as a solid.
[0442] MS: APCI(+ve) 409 (M+1)
[0443] .sup.1H NMR: .delta. (DMSO-d6) 8.00(d, 1H), 7.57(d, 1H),
7.53(d, 1H), 7.36-7.27(m, 5H), 7.20-7.16(m, 1H), 3.61(s, 2H),
3.55-3.47(m, 1H), 3.44(s, 2H), 2.69-2.66(m, 2H), 2.02(t, 2H),
1.67-1.64(m, 2H), 1.41-1.31(m, 2H).
MP: 97-99.degree. C.
EXAMPLE 99
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(4-fluorophenyl)acetamide
[0444] 121
[0445] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and 2-(4-fluorophenyl)acetic acid (0.108 g)
using the method of Example 94. Yield 0.15 g as a solid.
[0446] MS: APCI(+ve) 395 (M+1).
[0447] .sup.1H NMR: .delta. (DMSO-d6) 7.98(d, 1H), 7.57(d, 1H),
7.53(d, 1H), 7.30-7.25(m, 3H), 7.13-7.07(m, 2H), 3.54-3.48(m, 1H),
3.45(s, 2H), 3.37(s, 2H), 2.72-2.69(m, 2H), 2.02(t, 2H),
1.71-1.68(m, 2H), 1.44-1.34(m, 2H).
[0448] MP: 144-7.degree. C.
EXAMPLE 100
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[2-(2-pyrayl)-1,3-thiazol-4-yl]-
acetamide
[0449] 122
[0450] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and 2-[2-(2-pyrazinyl)-1,3-thiazol-4-yl]acetic
acid (0.155 g) using the method of Example 94. Yield 0.08 g as a
solid.
[0451] MS: APCI(+vc) 462 (M+1)
[0452] .sup.1H NMR: .delta. (DMSO-d6) 9.25(d, 1H), 8.74-8.71(m,
2H), 8.07(d, 1H), 7.64(s, 1H), 7.59-7.54(m, 2H), 7.31-7.28(m, 1H),
3.69(s, 2H), 3.59-3.54(m, 1H), 3.45(s, 2H), 2.74-2.71(m, 2H),
2.04(t, 2H), 1.76-1.74(m, 2H), 1.49-1.39(m, 2H).
[0453] MP: 186-9.degree. C.
EXAMPLE 101
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[(5-phenyl-2-pyrimidinyl)sulfan-
yl]acetamide
[0454] 123
[0455] The title compound was prepared from the product of Example
1 step (ii) (0.3 g) and 2-[(5-phenyl-2-pyrimidinyl)sulfanyl]acetic
acid (0.172 g) using the method of Example 94.
[0456] Yield 0.1 15 g as a solid.
[0457] MS: APCI(+ve) 487/9 (M+1)
[0458] .sup.1H NMR: .delta. (DMSO-d6) 8.96(s, 2H), 8.09(d, 1H),
7.78-7.75(m, 2H), 7.58-7.43(m, 5H), 7.28(dd, 1H), 3.91(s, 2H),
3.59-3.52(m, 1H), 3.44(s, 2H), 2.70(br d, 2H), 2.03(br t, 2H),
1.72(br d, 2H), 1.47-138(m, 2H).
[0459] MP: 157.degree. C.
EXAMPLE 102
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1,2,4oxadiazol-
-5-yl]propanamide
[0460] 124
[0461] The title compound was prepared from the product of Example
1 step (ii) (0.9 g) and
3-[3-(2-pyridinyl)1,2,4-oxadiazol-5-yl]propanoic acid (0.3 g) using
the method of Example 94.
[0462] Yield 0.074 g as a solid.
[0463] MS: APCI(+ve) 460/2 (M+1)
[0464] .sup.1H NMR: .delta. (DMSO-d6) 8.76-8.74(m, 1H),
8.05-7.99(m, 2H), 7.94(d, 1H), 7.61-7.56(m, 2H), 7.52(d, 1H),
7.28(dd, 1H), 3.56-3.48(m, 1H), 3.43(s, 2H), 3.19(t, 2H),
2.71-2.66(m, 4H), 2.03(t,2H), 1.69(brd, 2H), 1.42-133(m, 2H).
[0465] MP: 155.degree. C.
EXAMPLE 103
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-1H-benzimidazol-2-amine
[0466] 125
(i) Ethyl 4-(1H-benzimidazol-2-ylamino)-1-piperidinecarboxylate
[0467] A solution of 2-chlorobenzimidazole (1 g) and ethyl
4-amino-1-piperidinecarboxylate (2 g) in 1-methyl-2-pyrrolidinone
was heated at 130.degree. C. for 24 h The mixture was partitioned
between water and ethyl acetate, the organic layer washed with
water, dried and lo evaporated under reduced pressure. Purification
was by chromatography eluting with 1% triethylamine/5% methanol in
dichloromethane. Yield 0.630 g as a solid.
[0468] TOF MS ES+289.1652 (M+1)
(ii) N-(4-Piperidinyl)-1H-benzimidazol-2-amine, dihydrochlozide
salt
[0469] The product from step (i) (0.58 g) was heated under reflux
with 5M hydrochloric acid (20 ml) for 24 h The solvent was
evaporated under reduced pressure, the residue azeotroped with
toluene, washed with ether. Yield 0.58 g as a solid.
[0470] TOF MS ES+217.1452 (M+1)
(iii)
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-1H-benzimidazol-2-amine
[0471] Triethylamine (0.223 ml) was added to a stirred suspension
of the product from step (ii) (0.2 g) in dimethylformamide. After 5
min 3,4-dichlorobenzaldehyde (0.175 g) then sodium
triacetoxyborohydride (0.212 g) was added and the mixture stirred
at room temperature for 3 h The mixture was partitioned between 2M
hydrochloric acid and ether, the aqueous layer was basified with
aqueous sodium hydrogencarbonate and extracted with ethyl acetate.
The organic layer was dried and evaporated under reduced pressure.
The residue was triturated with ethyl acetate/ether and the solid
collected. Yield 0.045 g.
[0472] TOF MS ES+375.4257 (M+1)
[0473] .sup.1H NMR: .delta. (DMSO-d6) 10.6(br s, 1H), 7.60-7.56(m,
2H), 7.32(dd, 1H), 7.12-7.09(m, 2H), 6.86-6.83(m, 2H), 6.49(d, 1H),
3.55-3.49(m, 3H), 2.79-2.71(m, 2H), 2.13-1.91(m, 4H), 1.56-1.46(m,
2H).
[0474] MP: 125.degree. C.
EXAMPLE 104
2-{[1-(3,4-Dichlorobenzyl)-4-piperidinyl]amino}-N-(3methoxyphenyl)acetamid-
e, dihydrochloride salt
[0475] 126
[0476] 2-Chloro-N-(3-methoxyphenyl)-acetamide (0.241 g) was added
to a stirred solution of the product of Example 1 step (ii)
(dihydrochloride salt) (0.4 g), triethylamine (0.608 g) in
1-methyl-2-pyrrolidinone (5 ml). The reaction mixture was heated at
80.degree. C. for 6 h then partitioned between ethyl acetate and
brine. The organic layer was washed with brine, dried and
evaporated under reduced pressure. Purification was by
chromatography eluting with
chloroform/isohexane/triethylamine/methano- l 30:15:3:0.5. The
resulting product was converted to the hydrochloride salt using
ethereal hydrogenchloride. Yield 0.135 g.
[0477] TOF MS ES+422.1406 (M+1)
[0478] .sup.1H NMR: .delta. (DMSO-d6) 11.21(br s, 1H), 10.82(s,
1H), 9.53(br s, 2H), 7.95(s, 1H), 7.75(d, 1H), 7.60(d, 1H),
7.31-723(m, 2H), 7.15(d, 1), 6.70(dd, 1H), 4.28(br s, 2H), 3.97(br,
H), 3.73(s, 3H), 2.96(br, 2H), 228-2.05(m, 4H).
[0479] MP: 274-6.degree. C.
EXAMPLE 105
N-[1-(3,4-Dichlorebenzyl)-4-piperidinyl]-N'-(3,4-dichlorophenyl)urea
[0480] 127
[0481] 3,4-Dichlorophenyl isocyanate (0.081 g) was added to a
stirred solution of the product from Example 1 step (ii) (0.13 g),
diisopropylethylamine (0.2 g) in dichloromethane (4 ml). The
reaction mixture was stirred for 20 h and the solvent removed under
reduced pressure. Purification was by chromatography eluting with
5% methanol/dichloromethane. Yield 0.09 g as a solid.
[0482] TOF MS ES+446.0360 (M+1)
[0483] .sup.1H NMR: .delta. (DMSO-d6) 8.65(s, 1H), 7.82(d, 1H),
7.59(d, 1H), 7.54(s, 1H), 7.31 (d, 1H), 7.22(dd, 1H), 6.26(d, 1H),
3.45(br s, 3H), 2.67(m, 2H), 2.1 1(m, 2H), 1.81(m, 2H), 1.40(m,
2H).
[0484] MP: 189-190.degree. C.
EXAMPLE 106
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N'-(3-methoxyphenyl)urea
[0485] 128
[0486] 3-Methoxyphenyl isocyanate (0.064 g) was added to a stirred
solution of the product from Example 1 step (ii) (0.13 g),
diisopropylethylamine (0.2 g) in dichloromethane (4 ml). The
reaction mixture was stirred for 20 h and the solvent removed under
reduced pressure. Purification was by chromatography eluting with
5% methanol/dichloromethane. Yield 0.09 g as a solid.
[0487] MS: APCI(+ve) 408/10 (M+1)
[0488] .sup.1H NMR: .delta. (DMSO-d6) 8.32(s, 1H), 7.59(d, 1H),
7.55(d, 1H), 7.31(dd, 1H), 7.13(m, 1H), 7.09(d, 1H), 6.83(dd,1H),
6.47(dd, 1H), 6.09(d, 1H), 3.69(s, 3H), 3.46(m, 3H), 2.66(m, 2H),
2.13(m, 2H), 1.81(m, 2H), 1.42(m, 2H).
[0489] MP: 178-9.degree. C.
EXAMPLE 107
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-N-(4-methoxybenzyl)amine,
dihydrochloride salt
[0490] 129
[0491] The title compound was prepared from the product of Example
1 step (ii) (0.185 g) and 4-methoxybenzaldehyde (0.49 ul) using the
method of Example 1 step (i). Yield 0.84 g as a solid.
[0492] MS: APCI(+ve) 379/81 (M+1)
[0493] .sup.1NMR: .delta. (DMSO-d6) 11.33(br s, 1H), 9.56(br s,
2H), 7.96 (s, 1H), 7.74(d, 1H), 7.61(d, 1H), 7.52(d, 1H), 6.97(d,
1H), 4.27(s, 2H), 4.07(s,2H), 3.77(s, 3H), 3.39-2.94(m, 5H),
2.32-2.28(m, 2H), 2.15-2.07(m, 2H).
[0494] MP: >250.degree. C.
[0495] The following table lists Examples 108-348 which are of
compounds of formula (I) all of which accord to formula (Ib).
1 (Ib) 130 Example R.sup.1 (Q).sub.m n R.sup.6 108 phenyl m = 0 2
3,4-Cl.sub.2--C.sub.6H.sub.3 109 4-Br--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 110 4-NH.sub.2--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 111 2-Br--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 112 4-F--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 113 3-CH.sub.3--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 114 2-CH.sub.3--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 115 3-Cl-4-OH--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 116 2-NO.sub.2--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 117 2-Cl--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 118 4-Cl--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 119 3,4-(OH).sub.2--C.sub.6H.sub.3 m =
0 2 3,4-Cl.sub.2--C.sub.6H.sub.3 120 4-NO.sub.2--C.sub.6H.sub.4 m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 121 phenyl m = 0 4
3,4-Cl.sub.2--C.sub.6H.sub.4 122 3,4-(OCH.sub.3).sub.2--C.sub.6H.s-
ub.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 123
3-F-4-OH--C.sub.6H.sub.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 124
3,4-methylenedioxyphenyl m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 125
4-OH--C.sub.6H.sub.4 m = 0 2 3,4-Cl.sub.2--C.sub.6H.sub.3 126
4-OH--C.sub.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 127
4-phenyl-phenyl m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 128
3,4-Cl.sub.2--C.sub.6H.sub.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3
129 3-OH--C.sub.6H.sub.4 m = 0 2 3,4-Cl.sub.2--C.sub.6H.sub.4 130
4-CH.sub.3--C.sub.6H.sub.4 m = 0 2 3,4-Cl.sub.2--C.sub.6H.sub.3 131
4-NO.sub.2--C.sub.6H.sub.4 m = 0 3 3,4-Cl.sub.2--C.sub.6H.sub.3 132
3,4-(OCH.sub.3).sub.2--C.sub.6H.sub.3 m = 0 2
3,4-Cl.sub.2--C.sub.6H.sub.3 133 C.sub.6F.sub.5 m = 0 2
3,4-Cl.sub.2--C.sub.6H.sub.3 134 4-CH.sub.3--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 135 4-OCF.sub.3--C.sub.6H.sub.4 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 136 3,4-(OCH.sub.3).sub.2--C.sub.-
6H.sub.3 m = 0 3 3,4-Cl.sub.2--C.sub.6H.sub.3 137
4-OCH.sub.3--C.sub.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3
138 4-N(CH.sub.3).sub.2--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.su- b.3 139 4-OCH.sub.3--C.sub.6H.sub.4 m =
0 2 3,4-Cl.sub.2--C.sub.6H.- sub.3 140
3,4,5-(OCH.sub.3).sub.3--C.sub.6H.sub.2 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 141 3,4-methylenedioxyphenyl m = 0 2
3,4-Cl.sub.2--C.sub.6H.sub.3 142 3-NH.sub.2--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 143 naphth-1-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 144 3-OCH.sub.3-4-OH--C.sub.6H.sub.3 m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 145 3-(6-Br-1-(prop-2-en-1- m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 yl)-naphth-2- ylaxymethyl)phenyl
146 4-(4-NO.sub.2--C.sub.6H.sub.4--CH.sub.2O)-- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 C.sub.6H.sub.4 147
3-F-4-CH.sub.3O--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 148 3-CH.sub.3--C.sub.6H.sub.4 m = 0 4
3,4-Cl.sub.2--C.sub.6H.sub.3 149 3-OH--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 150
4-(C.sub.6H.sub.5--CH.sub.2O)--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 151 4-(3-NO.sub.2--C.sub.6H.sub.4)--C-
.sub.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 152
2,5-(CH.sub.3).sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 153 4-I--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 154 3-Br--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 155
2-CH.sub.3-3-NO.sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.- 3 156 3-OH-4-OCH.sub.3--C.sub.6H.sub.3
m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 157 3-F--C.sub.6H.sub.4 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 158 2-F--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 159 3,5-(OCH.sub.3).sub.2--C.sub.6H.s-
ub.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 160 3-Cl--C.sub.6H.sub.4
m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 161 phenyl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 162 3,5-(CH.sub.3).sub.2--C.sub.6H.su-
b.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 163
3-OCH.sub.3--C.sub.6H.sub.4 m = 0 2 3,4-Cl.sub.2--C.sub.6H.sub.3
164 2,4-F.sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 165 2-OCH.sub.3--C.sub.6H.sub.4 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 166 3,4-F.sub.2--C.sub.6H.sub.3 m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 167 3,5-F.sub.2--C.sub.6H.sub.3 m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub- .3 168 Pyridin-3-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 169 Pyridin-2-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 170 5-Br-pyridin-3-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 171
2,4-(OCH.sub.3).sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.- 3 172 4-(benzyloxy)phenyl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 173 3-(benzyloxy)phenyl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 174 2-methyl-naphth-1-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 175
2-CH.sub.3CH.sub.2O--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 176
3,4-(OCH.sub.3).sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 177 4-CH.sub.3(CH.sub.2).sub.3O--C.su-
b.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 178 Indol-1-yl m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 179 2-NO.sub.2--C.sub.6H.sub.4 m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 180 Thien-2-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 181 3-Cl-4-OH--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 182 2,4-Cl.sub.2--C.sub.6H.sub.3 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 183 2,6-Cl.sub.2--C.sub.6H.sub.3 m =
0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 184 2-Br--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 185 3,4-Cl.sub.2--C.sub.6H.sub.3 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 186 3-Br--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 187 3,5-F.sub.2--C.sub.6H.sub.3 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 188 3-NH.sub.2--C.sub.6H.sub.4 m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 189 2-(ClCH.sub.2C(O)NH)- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 thiazol-4-yl 190 3-Cl--C.sub.6H.sub.4
m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 191
2,5-(OCH.sub.3).sub.2--C.sub.6H.sub.3 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.- 3 192 4-OH--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 193 Indol-3-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 194 5-OCH.sub.3-indol-3-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 195 Naphth-2-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 196 4-CH.sub.2--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 197
3,4,5-(OCH.sub.3).sub.3--C.sub.6H.sub.2 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 198 4-CH.sub.3(CH.sub.2).sub.3O--C.su-
b.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 199
4-S(O).sub.2CH.sub.3--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 200
2,4,6-(CH.sub.3).sub.3--C.sub.6H.sub.2 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 201 4-F--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 202 2-(pyrazin-2-yl)-thiazol-4- m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 yl 203
2-CH.sub.3-5-(CH.sub.3).sub.2CH-indol- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub- .3 3-yl 204 5-(pyrrolidin-1-yl)- m = 0
1 3,4-Cl.sub.2--C.sub.6H.sub.3 tetrazol-2-yl 205
5-(4-CH.sub.3-phenyl)-tetrazol- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 2-yl 206 3,5-F.sub.2--C.sub.6H.sub.3 m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 207 3-OCH.sub.3--C.sub.6H.sub.4
m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 208 5-Cl-benzo[b]thiophen-3- m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 yl 209
3,4-Cl.sub.2--C.sub.6H.sub.3 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3
210 2-phenyl-5-methyl-thiazol- m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3
4-yl 211 4-OCF.sub.3--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 212 3-methyl-5-Cl- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 benzo[b]thiophen-2-yl 213 3-methyl- m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 benzo[b]thiophen-2-yl 214
2-NO.sub.2--C.sub.6H.sub.4 m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 215
3-NO.sub.2--1,2,4-triazol-1-yl m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3
216 3,4-(NO.sub.2).sub.2-5-CH.s- ub.3- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 pyrazol-1-yl 217
4-(CH.sub.3).sub.2CH(CH.sub.2).sub.2O--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 218 2,3-(CH.sub.3).sub.2-indol-5-yl m
= 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 219 3,5-(CH.sub.3).sub.2-4-Cl-p-
yrazol- m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 1-yl 220
3,5-(CH.sub.3).sub.2-4-NO.sub.2- m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 pyrazol-1-yl 221
2,4-(NO.sub.2).sub.2-imidazol-1-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 222 4-NO.sub.2--imidazol-1-yl m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 223 3,5-(CH.sub.3).sub.2--pyrazol-1-y-
l m = 0 1 3,4-Cl.sub.2--C.sub.6H.sub.3 224
4-CH.sub.3(CH.sub.2).sub- .5--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 225 2-CN--C.sub.6H.sub.4 m = 0 1
3,4-Cl.sub.2--C.sub.6H.sub.3 226 4-Cl--C.sub.6H.sub.4 O 1
4-Cl--C.sub.6H.sub.4 227 4-Cl--C.sub.6H.sub.4 O 1
2-Br--C.sub.6H.sub.4 228 4-Cl--C.sub.6H.sub.4 O 1
3-(CO.sub.2CH.sub.3)4-Br-- C.sub.6H.sub.4 229 4-Cl--C.sub.6H.sub.4
O 1 4-NO.sub.2--C.sub.6H.sub.4 230 4-Cl--C.sub.6H.sub.4 O 1
3-benzoyl-phenyl 231 4-Cl--C.sub.6H.sub.4 O 1 5-OCH.sub.3-
benzimidazol-2-yl 232 4-Cl--C.sub.6H.sub.4 O 1 4-Br--C.sub.6H.sub.4
233 4-Cl--C.sub.6H.sub.4 O 1 4-(1,2,3-thiadiazol- 4-yl)-phenyl 234
4-Cl--C.sub.6H.sub.4 O 1 4-CH.sub.3--C.sub.6H.sub.4 235
4-Cl--C.sub.6H.sub.4 O 1 4-(2,6-Cl.sub.2--
C.sub.6H.sub.3)CH.sub.2S(O).sub.2-- C.sub.6H.sub.4 236
4-Cl--C.sub.6H.sub.4 O 1 3,S-Br.sub.2--C.sub.6H.sub.3 237
4-Cl--C.sub.6H.sub.4 O 1 Indan-5-yl 238 4-Cl--C.sub.6H.sub.4 O 1
2-F-3-Cl--C.sub.6H.sub.3 239 4-Cl--C.sub.6H.sub.4 O 1
benzofurazan-5-yl 240 4-Cl--C.sub.6H.sub.4 O 1 7-Cl-quinolin-2-yl
241 4-F--C.sub.6H.sub.4 m = 0 1 2,5-Cl.sub.2--C.sub.6H.sub.3 242
4-F--C.sub.6H.sub.4 m = 0 1 2,3-Cl.sub.2--C.sub.6H.sub.3 243
4-F--C.sub.6H.sub.4 m = 0 1 4-F--C.sub.6H.sub.4 244
4-F--C.sub.6H.sub.4 m = 0 1 3-CO.sub.2CH.sub.3-4-Br--
C.sub.6H.sub.3 245 4-F--C.sub.6H.sub.4 m = 0 1
4-NO.sub.2--C.sub.6H.sub.4 246 4-F--C.sub.6H.sub.4 m = 0 1
3-benzoyl-phenyl 247 4-F--C.sub.6H.sub.4 m = 0 1
4-CH.sub.3-naphth-1-yl 248 4-F--C.sub.6H.sub.4 m = 0 1
3,4-methylene- dioxyphenyl 249 4-F--C.sub.6H.sub.4 m = 0 1
5-OCH.sub.3- benzimidazol-2-yl 250 4-F--C.sub.6H.sub.4 m = 0 1
3-NO.sub.2--4-CH.sub.3-- C.sub.6H.sub.3 251 4-F--C.sub.6H.sub.4 m =
0 1 3,4-(CH.sub.3).sub.2--C.sub.6H.sub.3 252 4-F--C.sub.6H.sub.4 m
= 0 1 3-CH.sub.3-4-OCH.sub.3-- C.sub.6H.sub.3 253
4-F--C.sub.6H.sub.4 m = 0 1 4-(2-C(O)NH.sub.2--
C.sub.6H.sub.4)--C.sub.6H.sub.4 254 4-F--C.sub.6H.sub.4 m = 0 1
4-Br--C.sub.6H.sub.4 255 4-F--C.sub.6H.sub.4 m = 0 I
4-(2,6-Cl.sub.2-- C.sub.6H.sub.4CH.sub.2S(O).sub.2-- C.sub.6H.sub.4
256 3-(pyridin-2-yl)-1,2,4- m = 0 2 4-Cl--C.sub.6H.sub.4
oxadiazol-5-yl 257 3-(pyridin-2-yl)-1,2,4- m = 0 2
3-Cl-4-OCH.sub.3--C.sub.6H.sub.3 oxadiazol-5-yl 258
3-(pyridin-2-yl)-1,2,4- m = 0 2 2,3-Cl.sub.2--C.sub.6H.sub.3
oxadiazol-5-yl 259 3-(pyridin-2-yl)-1,2,4- m = 0 2
4-F--C.sub.6H.sub.4 oxadiazol-5-yl 260 3-(pyridin-2-yl)-1,2,4- m =
0 2 3-CF.sub.3--C.sub.6H.sub.4 oxadiazol-5-yl 261
3-(pyridin-2-yl)-1,2,4- m = 0 2 4-NO.sub.2--C.sub.6H.sub.4
oxadiazol-5-yl 262 3-(pyridin-2-yl)-1,2,4- m = 0 2 3-benzoyl-phenyl
oxadiazol-5-yl 263 3-(pyridin-2-yl)-1,2,4- m = 0 2 3,4-methylene-
oxadiazol-5-yi dioxyphenyl 264 3-(pyridin-2-yl)-1,2,4- m = 0 2
3,5-(CH.sub.3).sub.2--C.sub.6H.sub.3 oxadiazol-5-yl 265
3-(pyridin-2-yl)-1,2,4- m = 0 2 3-NO.sub.2-4-CH.sub.3--
oxadiazol-5-yl C.sub.6H.sub.3 266 3-(pyridin-2-yl)-1,2,4- m = 0 2
3,4-(CH.sub.3).sub.2--C.sub.6H.sub.3 oxadiazol-5-yl 267
3-(pyridin-2-yl)-1,2,4- m = 0 2 3-CH.sub.3--C.sub.6H.sub.4
oxadiazol-5-yl 268 3-(pyridin-2-yl)-1,2,4- m = 0 2
3-CH.sub.3-4-OCH.sub.3-- oxadiazol-5-yl C.sub.6H.sub.4 269
3-(pyridin-2-yl)-1,2,4- m = 0 2 4-Br--C.sub.6H.sub.4 oxadiazol-5-yl
270 3-(pyridin-2-yl)-1,2,4- m = 0 2 Indan-5-yl oxadiazol-5-yl 271
3-(pyridin-2-yl)-1,2,4- m = 0 2 4-CF.sub.3--C.sub.6H.sub.4
oxadiazol-5-yl 272 3,4-pyridin-2-yl)-1,2,4- m = 0 2 Naphth-2-yl
oxadiazol-5-yl 273 3-(pyridin-2-yl)-1,2,4- m = 0 2
4-CH.sub.3--C.sub.6H.sub.4 oxadiazol-5-yl 274
3-(pyridin-2-yl)-1,2,4- m = 0 2 benzofurazan-5-yl oxadiazol-5-yl
275 3-(pyridin-2-yl)-1,2,4- m = 0 2 3,4-F.sub.2--C.sub.6H.sub.3
oxadiazol-5-yl 276 3-(pyridin-2-yl)-1,2,4- m = 0 2
7-Cl-quinolin-2-yl oxadiazol-5-yl 277 3-(pyridin-2-yl)-1,2,4- m = 0
2 3-Cl--C.sub.6H.sub.4 oxadiazol-5-yl 278 3-(pyridin-2-yl)-1,2,4- m
= 0 2 4-CF.sub.3--C.sub.6H.sub.4 oxadiazol-5-yl 279
3-(pyridin-2-yl)-1,2,4- m = 0 2 4-CH.sub.3--C.sub.6H.sub.4
oxadiazol-5-yl 280 4-OCH.sub.3--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 281 4-Cl--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 282 4-NO.sub.2--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 283 4-NHC(O)CH.sub.3--C.sub.6H.sub.4 O
1 3,4-Cl.sub.2--C.sub.6H.sub.3 284 4-O(CH.sub.2).sub.2CH.sub.3---
C.sub.6H.sub.4 O 1 3,4-Cl.sub.2--C.sub.6H.sub.3 285
3-CO.sub.2CH.sub.2CH.sub.3--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub- .3 286
2-C(CH.sub.3).sub.3--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 287 2-NHC(O)CH.sub.3--C.sub.6H.sub.4 O
1 3,4-Cl.sub.2--C.sub.6H.sub.3 288 3,5-(OCH.sub.3).sub.2--C.sub.-
6H.sub.3 O 1 3,4-Cl.sub.2--C.sub.6H.sub.3 289
2-OCH.sub.3-5-NO.sub.2--C.sub.6H.sub.3 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 290 4-CN--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 291 2-Cl-5-CF.sub.3--C.sub.6H.sub.3 O
1 3,4-Cl.sub.2--C.sub.6H.sub.3 292
2-NO.sub.2-5-CH.sub.3--C.sub.6H.sub.3 O 1
3,4-Cl.sub.2--C.sub.6H.sub.- 3 293 3-Cl-5-OCH.sub.3--C.sub.6H.sub.3
O 1 3,4-Cl.sub.2--C.sub.6H.s- ub.3 294 3-NO.sub.2--C.sub.6H.sub.4 O
1 3,4-Cl.sub.2--C.sub.6H.sub.- 3 295 3-Br--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 296 4-I--C.sub.6H.sub.4 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 297 3,5-F.sub.2--C.sub.6H.sub.3 O 1
3,4-Cl.sub.2--C.sub.6H.sub.3 298
4,6-(NH.sub.2).sub.2-pyrimidin-2-yl S I 3,4-F.sub.2--C.sub.6H.sub.3
299 Benzimidazol-2-yl S 1 3,4-F.sub.2--C.sub.6H.sub.3 300
Thiazol-2-yl S 1 3,4-F.sub.2--C.sub.6H.sub.3 301 131 S 1
3,4-F.sub.2--C.sub.6H.sub.3 302 5-NO.sub.2-benzimidazol-2- -yl S 1
3,4-F.sub.2--C.sub.6H.sub.3 303 Pyridin-2-yl S 1
3,4-F.sub.2--C.sub.6H.sub.3 304 132 S 1 3,4-F.sub.2--C.sub.6H.sub.3
305 1H-1,2,4-triazol-3-yl S I 3,4-F.sub.2--C.sub.6H.sub.3 306
Pyrimidin-2-yl S I 3,4-F.sub.2--C.sub.6H.sub.3 307
1-phenyl-tetrazol-5-yl S 1 3,4-F.sub.2--C.sub.6H.sub.3 308
4,6-(CH.sub.3).sub.2-pyrimidin-2-y- l S 1
3,4-F.sub.2--C.sub.6H.sub.3 309 4-(thiophen-2-yl)- S 1
3,4-F.sub.2--C.sub.6H.sub.3 pyrimidin-2-yl 310
2-(cyclopropyl-CH.sub.2S)- S 1 3,4-F.sub.2--C.sub.6H.sub.3
1,3,4-thiadiazol-5-yl 311 4-methyl-3-(thiophen-2- S 1
3,4-F.sub.2--C.sub.6H.sub.3 yl)-1,2,4-triazol-5-yl 312
3-CN-6-(CH.sub.3C(O))- S 1 3,4-F.sub.2--C.sub.6H.sub.3 pyridin-2-yl
313 1H-pyrazolo[3,4- S 1 3,4-F.sub.2--C.sub.6H.sub.3
d]pyrimidin-4-yl 314 5-OCH.sub.3-benzimidazol-2- S 1
3,4-F.sub.2--C.sub.6H.sub.3 315 5-F-6-Cl-benzimidazol-2- S 1
3,4-F.sub.2--C.sub.6H.sub.3 yl 316 4,5-dihydrothiazol-2-yl S 1
3,4-F.sub.2--C.sub.6H.sub.3 317 1H-5-phenyl-1,2,4-triazol- S 1
3,4-F.sub.2--C.sub.6H.sub.3 3-yl 318 2-(thiophen-2-yl)-1,3,4- S 1
3,4-F.sub.2--C.sub.6H.sub.3 oxadiazol-5-yl 319 Quinoxalin-2-yl S 1
3,4-F.sub.2--C.sub.6H.sub.3 320 2,5-Cl.sub.2--C.sub.6H.sub.3 S 1
3,4-F.sub.2--C.sub.6H.sub.3 321 2-(pyridin-2-yl)-1,3,4- S 1
3,4-F.sub.2--C.sub.6H.sub.3 oxadiazol-5-yl 322
7-CF.sub.3-quinolin-4-yl S 1 3,4-F.sub.2--C.sub.6H.sub.3 323
2-(pyridin-2-yl)-4-CH.sub.3- S 1
3,4-F.sub.2--C.sub.6H.sub.3 pyrimidin-6-yl 324 Naphth-1-yl S 1
3,4-F.sub.2--C.sub.6H.sub.3 325 3,4-(OCH.sub.3).sub.2--C.sub.6-
H.sub.3 S 1 3,4-F.sub.2--C.sub.6H.sub.3 326 1,3,4-thiadiazol-2-yl S
1 3,4-F.sub.2--C.sub.6H.sub.3 327 3-CF.sub.3--C.sub.6H.sub.4 S 1
3,4-F.sub.2--C.sub.6H.sub.3 328 133 S 1 3,4-F.sub.2--C.sub.6H.sub.3
329 3,4-Cl.sub.2--C.sub.6H.su- b.3 S 1 3,4-F.sub.2--C.sub.6H.sub.3
330 3-CN-5-CH.sub.3-pyridin-2-y- l S 1 3,4-F.sub.2--C.sub.6H.sub.3
331 4-phenyl-thiazol-2-yl S 1 3,4-F.sub.2--C.sub.6H.sub.3 332 134 S
1 3,4-F.sub.2--C.sub.6H.sub.3 333 2-CH.sub.3-1,3,4-thiadiaz- ol-5-
S 1 3,4-F.sub.2--C.sub.6H.sub.3 yl 334 135 S 1
3,4-F.sub.2--C.sub.6H.sub.3 335 136 S 1 3,4-F.sub.2--C.sub.6H.sub.3
336 2-phenoxy-phenyl S 1 3,4-F.sub.2--C.sub.6H.sub.3 337
2-OCH.sub.3--C.sub.6H.sub.4 S 1 3,4-F.sub.2--C.sub.6H.sub.3 338
2-CH.sub.3-4-Cl--C.sub.6H.sub.3 S 1 3,4-F.sub.2--C.sub.6H.sub.3 339
2-CH.sub.3-6-Cl--C.sub.6H.sub.3 S 1 3,4-F.sub.2--C.sub.6H.sub.3 340
2-(HC.ident.C--CH.sub.2S)-1,3,- 4- S 1 3,4-F.sub.2--C.sub.6H.sub.3
thiadiazol-5-yl 341 2-CO.sub.2CH.sub.3--C.sub.6H.sub.4 S 1
3,4-F.sub.2--C.sub.6H.sub.3 342 4-CN--C.sub.6H.sub.4 O 1
3,4-F.sub.2--C.sub.6H.sub.3 343
4-((CH.sub.3).sub.2NCH.sub.2)--C.sub.6H.sub.4 O 1
3,4-F.sub.2--C.sub.6H.s- ub.3 344 137 O 1
3,4-F.sub.2--C.sub.6H.sub.3 345 3-CH.sub.2OH--C.sub.6H.sub.4 O 1
3,4-F.sub.2--C.sub.6H.sub.3 346
2-OCH.sub.2CH.sub.2OH--C.sub.6H.sub.4 O 1
3,4-F.sub.2--C.sub.6H.sub.3 347
4-CH.sub.3(CH.sub.2).sub.2O--C.sub.6H.sub.4 O 1
3,4-F.sub.2--C.sub.6H.sub.3 348 3-Cl-5-OCH.sub.3--C.sub.6H.sub.3 O
1 3,4-F.sub.2--C.sub.6H.sub.3
GENERAL PREPARATION OF EXAMPLES 108-225
[0496] PyBroP.RTM. (bromo-tris-pyrrolidino-phosphonium
hexafluorophosphate, 2 equivalents) was added to a solution of the
product from Example 1 step (ii) (hydrochloride salt, 1 mg) the
appropriate acid (2 equivalents) and triethylamine in
1-methyl-2-pyrrolidone (0.2 ml) and was left for 24 h. The reaction
mixture was evaporated to dryness and the residue was dissolved in
dimethylsulfoxide (0.3 ml).
GENERAL PREPARATION OF EXAMPLES 225-240
Step i: tert-Butyl
4-{[(4-chlorophenoxy)acetyl]amino}-1-piperidinecarboxyl- ate
[0497] Prepared following the method of Example 94 using
(4-chlorophenoxy)acetic acid (0.50 g), 1,1-carbonyldiimidazole
(0.50 g) and tert-butyl 4-amino-1-piperidinecarboxylate (0.46 g) to
give the subtitle compound (0.54 g).
[0498] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 134-1.40 (2H,
m), 1.46 (9H, s), 1.90-1.95 (2H, m), 2.86-2.88 (2H, m), 4.01-4.14
(3H, m), 4.45 (2H, s), 638-6.41 (1H, m), 6.84-6.87 (2H, m),
726-7.30 (2H, m).
Step ii: 2-(4chlorophenoxy)-N-(4-piperidinyl)acetamide
[0499] Prepared following the method of Example 1 step (ii) using
tert-butyl
4-{[(4-chlorophenoxy)acetyl]amino}-1-piperidinecarboxylate (0.52 g)
to give the subtitle compound (0.35 g).
[0500] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.32-1.45 (2H,
m), 1.93-1.97 (2H, m), 2.68-2.77 (2H, m), 3.07-3.11 (2H, m),
3.91-4.04 (1H, m), 4.45 (2H, s), 6.38-6.40 (1H, m), 6.84-6.89 (2H,
m), 7.26-7.31 (2H, m).
Step iii: Final Product
[0501] A mixture of the product from step (ii) (1.07 mg), the
appropriate alkyl halide (2 equivalents) and
N,N-diisopropylethylamine (3 equivalents) in
1-methyl-2-pyrrolidinone (0.18 ml) was left at room temperature for
24 h. The mixture was evaporated to dryness and the residue was
dissolved in dimethylsulfoxide (0.4 ml).
GENERAL PREPARATION OF EXAMPLES 241-255
[0502] A mixture of 2-(4-fluorophenyl)-N-(4-piperidinyl)acetamide
(WO97/36871; 0.94 mg), the appropriate alkyl halide (2 equivalents)
and N,N-diisopropylethylamine (3 equivalents) in
1-methyl-2-pyrrolidinone (0.18 ml) was left at room temperature for
24 h. The mixture was evaporated to dryness and the residue was
dissolved in dimethylsulfoxide (0.4 ml).
GENERAL PREPARATION OF EXAMPLES 256-279
Step i: tert-Butyl
4-({3-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]propanoyl}a-
mino)-1-piperidinecarboxylate
[0503] 3-[3-(2-Pyridinyl)-1,2,4-oxadiazol-5-yl]propanoic acid (0.60
g) was dissolved in dichloromethane (10 ml).
1,1-Carbonyldiimidazole (0.33 g) was added followed by tert-butyl
4-amino-1-piperidinecarboxylate hydrochloride (0.5 g) and
triethylamine (0.31 ml). After 2 hours water, brine and
dichloromethane were added and the phases separated. The organic
phase was dried, filtered and evaporated and the residue was
purified by chromatography eluting with ethyl acetate:methanol
(33:1) to give the subtitle compound (0.40 g).
[0504] .sup.1H NMR (399.98 MHz, DMSO) .delta. 1.22-1.24 (2H, m),
1.39 (9H, s), 1.62-1.71 (2H, m), 2.66-2.71 (4H, m), 3.18-3.23 (2H,
m), 3.65-3.83 (3H, m), 7.58-7.63 (1H), 8.01-8.04 (3H, m),
8.74-8.76(1H, m).
Step ii:
N-(4-Piperidinyl)-3-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]propana-
mide
[0505] tert-Butyl
4-({3-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]propanoyl}am-
ino)-1-piperidinecarboxylate (0.40 g) was dissolved in
dichloromethane (6 ml) and trifluoroacetic acid (3 ml) was added.
After 2 hours water, 2N sodium hydroxide and dichloromethane were
added and the phases were separated. The organic phase was dried,
filtered and evaporated to give the subtitle compound (0.19 g).
[0506] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.35-1.45 (2H,
m), 1.86-1.97 (2H, m), 2.69-2.84 (4H, m), 3.09-3.13 (2H, m),
3.32-3.36 (2H, m), 3.86-3.95 (1H, m), 5.82-5.84 (1H, m), 7.42-7.45
(1H, m), 7.83-7.87 (1H, m), 8.10-8.12 (1H, m), 8.78-8.79 (1H,
m).
Step iii: Final Product
[0507] A mixture of the product from step (ii) (1.21 mg), the
appropriate alkyl halide (2 equivalents) and
N,N-diisopropylethylamine (3 equivalents) in
1-methyl-2-pyrrolidinone (0.18 ml) was left at room temperature for
24 h. The mixture was evaporated to dryness and the residue was
dissolved in dimethylsulfoxide (0.4 ml).
GENERAL PREPARATION OF EXAMPLES 280-296
Step i:
2Chloro-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]acetamide
[0508] Prepared following the general preparation method of
Examples 297-357 step (iii) using
1-(3,4-dichlorobenzyl)-4-piperidinamine hydrochloride (2.0 g),
N,N-diisopropylethylamine (5.55 ml) and chloroacetyl chloride (0.55
ml) to give the subtitle compound (1.0 g).
[0509] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.48-1.61 (2H,
m), 1.91-1.94 (2H, m), 1.95-2.18 (2H, m), 2.77-2.80 (2H, m), 3.44
(2H, s), 3.78-3.87 (1H, m), 4.04 (2H, s), 7.13-7.16 (1H, m),
7.37-7.43 (2H, m).
Step ii: Final Product
[0510] A mixture of the product from step (i) (1.34 mg), the
appropriate phenol (1.5 equivalents) and potassium tert-butoxide
(1.4 equivalents) in 1-methyl-2-pyrrolidinone (0.13 ml) was left at
room temperature for 24 hours. The mixture was evaporated to
dryness and the residue was dissolved in dimethylsulfoxide (0.4
ml).
GENERAL PREPARATION OF EXAMPLES 297-340
Step i: Carbamic acid,
[1-[(3,4-difluorophenyl)methyl]-4-piperidinyl]-, 1,1-dimethylethyl
ester
[0511] Carbamic acid, 4-piperidinyl-, 1,1-dimethylethyl ester (6.95
g) was dissolved in N,N-dimethylformamide (70 ml).
3,4-Difluorobenzylbromide (4.55 ml) and potassium carbonate (16.0
g) were added The mixture was heated to reflux for 16 hours, then
allowed to cool to room temperature. Ammonium chloride solution was
added and the mixture was extracted thrice with ethyl acetate. The
organic phases were washed with water (twice) and brine, then
dried, filtered and evaporated. The residue was triturated with
ether:iso-hexane (1:1) to give the subtitle compound (8.13 g)
[0512] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.36-1.43 (m,
2H), 1.44 (s, 9H), 1.91 (d, J=11.8 Hz, 2H), 2.08 (td, J=11.4, 2.7
Hz, 2H), 2.75 (d, J=11.3 Hz, 2H), 3.41 (s, 2H), 3.42-3.55 (m, 1H),
4.38-4.47 (m, 1H), 6.96-7.02 (m, 1H), 7.04-7.11 (m, 2H), 7.13-7.19
(m, 1H)
Step ii: 1-[(3,4-Difluorophenyl)methyl]-piperidin-4-ylamine
dihydrochloride
[0513] Carbamic acid,
[1-[(3,4-difluorophenyl)methyl)-4-piperidinyl]-, 1,1-dimethylethyl
ester was suspended in 6N hydrochloric acid (100 ml). After 16
hours excess hydrochloric acid was evaporated and the residue
azeotroped with toluene, dried and evaporated to give the subtitle
compound (8.10 g).
[0514] .sup.1H NMR (399.98 MHz, DMSO) .delta. 1.91-2.01(2H,m),
2.31-2.47(2H,m), 2.86-3.20(2H,m), 3.54-3.66(3H,m), 4.75-4.83(2H,s),
7.26-7.61(3H,m).
Step iii:
2-Chloro-N-[1-[(3,4-difluorophenyl)methyl]-piperidin-4-yl]-aceta-
mide
[0515] 1-[(3,4-Difluorophenyl)methyl]-piperidin-4-ylamine
dihydrochloride (3.18 g) was dissolved in tetrahydrofuran (40 ml).
Diisopropylethylamine (6.84 g) and chloroacetyl chloride (1.33 g)
were added. After 3 hours water, brine and ethyl acetate were added
the phase were separated. The organic phase was dried, filtered and
evaporated and the residue was purified by chromatography eluting
with ethyl acetate to give the subtitle compound (0.728 g).
[0516] .sup.1H NMR (CDCl.sub.3) .delta. 1.46-1.59 (2H, m), 1.93
(2H, dt), 2.14 (2H, td), 2.78 (2H, d), 3.43 (2H, s), 3.76-3.91 (1H,
m), 4.04 (2H, s), 639-6.51 (1H, m), 6.98-7.02 (1H, m), 7.08 (1H,
dd), 7.17 (1H, ddd).
Step iv: Final Product
[0517] The product from step (iii) (1.21 mg) was dissolved in
dimethylsulfoxide (50 .mu.l) and diisopropylethylamine (1.55 mg, 3
equivalents) was added as a solution in dimethylsulfoxide (50
.mu.l). The appropriate thiol was added (1 equivalent) in
dimethylsulfoxide (40 .mu.l) and the reaction mixture was left at
room temperature for 24 hours. The reaction mixture was evaporated
to dryness and the residue was dissolved in dimethylsulfoxide (400
.mu.l).
GENERAL PREPARATION OF EXAMPLES 341-348
[0518] Prepared from the product of general preparation for
Examples 297-340 step (iii) and the appropriate phenol following
the method of Examples 280-296 step (ii).
EXAMPLE 351
3-[3-(4-Bromo-1-methyl-1H-pyrazol-3-yl)-1,2,4-oxadiazol-5-yl]-N-[1-(3,4-di-
chlorobenzyl)-4-piperidinyl]propanamide
[0519] 138
Step i: Methyl
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-4-oxobutano-
ate
[0520] To a solution of 1-(3,4-dichlorobenzyl)-4-piperidinamine
hydrochloride (3.50 g) in to dichloromethane (100 ml) was added
methyl 4-chloro-4-oxobutanoate (2.00 g) dropwise. Triethylamine
(3.90 g) was added and the reaction stirred under nitrogen for 2
hours. Saturated sodium hydrogen carbonate solution was then added,
with the solution being extracted three times with dichloromethane.
The pooled organic phase was washed once with water, once with
saturated brine and dried over anhydrous magnesium sulfate. After
filtration the solvent was removed under reduced pressure to leave
methyl 4-{[1-(3,4-dichlorobenzyl)-
-4-piperidinyl]amino}-4-oxobutanoate (3.00 g).
[0521] MS (+veES) 373 ((M+H).sup.+)
Step ii: Lithium
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-4-oxobuta-
noate
[0522] To a solution of methyl
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]am- ino}-4-oxobutanoate
(3.72 g) in methanol (30 ml) was added lithium hydroxide (0.41 g)
in water (10 ml) which was stirred under nitrogen for 48 hours. The
solvent was removed under reduced pressure, the residue was
triturated with ether and filtered to leave lithium
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-4-oxobutanoate
(3.50 g).
[0523] MS (+veES) 359 ((M+H).sup.+)
Step iii:
3-[3-(4-Bromo-1-methyl-1H-pyrazol-3-yl)-1,2,4-oxadiazol-5-yl]-N--
[1-(3,4-dichlorobenzyl)-4-piperidinyl]propanamide
[0524] To lithium
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-4-oxobut- anoate
(0.292 g) in dichloromethane (6 ml) was added dimethylformamide
(1.5 ml), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (0.183 g), 1-hydroxybenzotriazole hydrate (0.130 g),
4-bromo-N'-hydroxy-1-methyl-1H-pyrazole-3-carboximidamide (0.175 g)
and triethylamine (0.161 g). Reaction was left to stir for 24 hours
before removal of dichloromethane under reduced pressure. Pyridine
(5 ml) was added and heated at reflux for 5 hours. Pyridine was
removed under reduced pressure, followed by the addition of water.
The solution was extracted three times with dichloromethane. The
pooled organic phase was washed once with water, once with
saturated brine and dried over magnesium sulfate. After filtration
the product was azeotroped twice with toluene and was purified by
reverse phase hplc (RPHPLC; 75%-5%, 0.1% ammonium
acetate/acetonitrile). Solvent was removed under reduced pressure
to give the titled compound (0.164 g).
[0525] MS (+veAPC) 543 ((M+H).sup.+)
[0526] .sup.1H NMR (DMSO): .delta. 8.21-8.17(1H,m);
7.95-7.76(1H,m); 7.60-7.54(1H,m); 7.35-7.25(1H,m); 4.35-4.21(1H,m);
3.93(2H,s); 3.44-3.35(2H,m); 3.19-3.14(3H,m); 2.73-2.64(2H,m);
2.58(3H,s); 2.00-1.89(2H,m); 1.73-1.60(2H,m); 1.36-1.24(1H,m).
EXAMPLE 352
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyrazinyl)-1,2,4-oxadiazo-
l-5-yl]propanamide
[0527] 139
[0528] To lithium
4-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}-4-oxobut- anoate
(Example 351, step ii) (0.292 g) in dichloromethane (6 ml) was
added N,N-dimethylformamide (1.5 ml),
1-(3-ethylaminopropyl)-3-ethylcarbo- diimide hydrochloride (0.183
g), 1-hydroxybenzotriazole hydrate (0.130 g),
N'-hydroxy-2-pyrazinecarboximidamide (0.110 g) and triethylamine
(0.161 g). The reaction mixture was left to stir for 24 hours
before removal of dichloromethane under reduced pressure. Pyridine
(5 ml) was added and heated at reflux for 5 hours. Pyridine was
removed under reduced pressure followed by the addition of water.
The solution was extracted three times with dichloromethane. The
pooled organic phase was washed once with water, once with
saturated brine and dried over magnesium sulfate. After filtration
the product was azeotroped twice with toluene and was purified by
RPHPLC (75%-5%, 0.1% ammonium acetate/acetonitrile). Solvent was
removed under reduced pressure to give the title compound (0.067
g).
[0529] MS (+veAPC) 461 ((M+H).sup.+)
[0530] .sup.1H NMR (DMSO) .delta. 9.23(1H,s); 8.81-8.45(2H,m);
7.96-7.94(1H,m); 7.58-7.56(1H,m); 7.53-7.52(1H,m); 7.29-7.26(1H,m);
3.55-3.48(1H,m); 3.43(2H,s); 3.24-3.20(2H,m); 2.71-2.68(4H,m);
2.03-1.98(2H,m); 1.70-1.68(2H,m); 1.42-1.33(2H,m).
EXAMPLE 353
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-{3-[(2-thienylsulfonyl)methyl]--
1,2,4-oxadiazol-5-yl}propanamide hydrochloride
[0531] 140
Step i:
3-{3-[(2-Thienylsulfonyl)methyl]-1,2,4-oxadiazol-5-yl}propanoic
acid
[0532] (1Z)-N'-hydroxy-2-(2-thienylsulfonyl)ethanimidamide (0.250
g) with dihydro-2,5-furandione (0.114 g) in dimethylformamide (0.2
ml) was heated at 120.degree. C. for 2 hours. The reaction was
allowed to cool and triturated with diethyl ether and filtered to
leave
3-{3-[(2-thienylsulfonyl)methyl]-1,2,4-oxadiazol-5-yl}propanoic
acid (0.332 g).
[0533] MS (+veES) 303 ((M+H).sup.+)
Step ii:
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-{3-[(2-thienylsulfonyl-
)methyl]-1,2,4-oxadiazol-5-yl}propanamide hydrochloride
[0534]
3-{3-[(2-Thienylsulfonyl)methyl]-1,2,4-oxadiazol-5-yl}propanoic
acid (0.332 g) in dichloromethane was stirred under nitrogen.
Oxalyl chloride (0.252 g) was added dropwise followed by the
addition of one drop of dimethylformamide. After 30 minutes the
solvent and oxalyl chloride was removed under reduced pressure
followed by the addition of dichloromethane (10 ml),
1-(3,4-dichlorobenzyl)-4-piperidinamine hydrochloride (0.347 g),
and triethylamine (0.202 g) and allowed to stir for 2 hours under
nitrogen. Saturated sodium hydrogen carbonate was added to the
reaction with the resulting solution being extracted three times
with dichloromethane. The pooled organic phases were washed once
with water, once with brine, dried over magnesium sulfate, filtered
and the solvent removed under reduced pressure to leave a brown
oil. This oil was purified by RPHPLC (75%-5%, 0.1% ammonium
acetate/acetonitrile) followed by chromatography using 3%
ethanol/dichloromethane. The solvent was removed under reduced
pressure, followed by the addition of hydrogen chloride in diethyl
ether, filtered and dried to leave
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-{3-[(2-thienylsulfonyl)methyl]-
-1,2,4-oxadiazol-5-yl}propanamide hydrochloride (0.04 g) as a pale
yellow solid.
[0535] MS (+veES) 545 ((M+H).sup.+)
[0536] .sup.1H NMR (DMSO) .delta. 10.51(1H,s); 8.21-8.13(2H,m);
7.91(1H,s); 7.77-7.71(2H,m); 7.58-7.55(1H,m); 7.28-7.26(1H,m);
5.07-5.05(2H,m); 4.26-4.25(2H,m); 3.92(1H,m); 3.34-3.31(2H,m);
3.15-3.08(2H,m); 3.02-2.94(2H,m); 2.60-2.58(2H,m); 1.92-1.84(2H,m);
1.80-1.70(2H,m).
EXAMPLE 354
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(4-pyridinyl)-1,2,4-oxadiazo-
l-5-yl]propanamide
Step i: 3-[3-(4-Pyridinyl)-1,2,4-oxadiazol-5-yl]propanoic acid
[0537] N'-hydroxy-4-pyridinecarboximidamide (0.300 g) with
dihydro-2,5-furandione (0.217 g) in dimethylformamide (2 drops) was
heated for 4 times 30 seconds in a CEM MARS 5 microwave at 100% of
300 W to leave a fused mass. The reaction was allowed to cool and
triturated with ethanol and filtered to leave
3-[3-(4-pyridinyl)-1,2,4-oxadiazol-5-y- l]propanoic acid (0.241
g).
[0538] MS (+veES) 220 ((M+H).sup.+)
Step ii:
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(4-pyridinyl)-1,2,4-
-oxadiazol-5-yl]propanamide
[0539] For method refer to Example 353 step ii
[0540] Purification was performed via chromatography (2.5%
ethanol/dichloromehane). Solvent removed under reduced pressure to
leave
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-[3-(4-pyridinyl)-1,2,4-oxadiaz-
ol-5-yl]propanamide (0.154 g) as a pale cream solid.
[0541] MS (+veES) 460 ((M+H).sup.+)
[0542] .sup.1H NMR (DMSO) .delta. 8.81-8.79(2H,m); 7.96-7.90(3H,m);
7.60-7.56(2H,m); 7.30-7.27(1H,m); 3.53-3.51(1H,m); 3.44(2H,s);
3.23-3.19(2H,m); 2.71-2.68(4H,m); 2.05-1.97(2H,m); 1.71-1.67(2H,m);
1.44-1.32(2H,m).
EXAMPLE 355
Cis-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1,2,4-oxad-
iazol-5-yl]cyclopropanecarboxamide
Step i:
Cis-2-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]cyclopropanecarboxylic
acid
[0543] N'-hydroxy-2-pyridinecarboximidamide (0.137 g) with
3-oxabicyclo[3.1.0]hexane-2,4-dione (0.112 g) in dimethylformamide
(2 drops) was heated for 4 times 30 seconds in a CEM MARS 5
microwave at 100% of 300 W to leave a fused mass. The reaction was
allowed to cool and triturated with diethyl ether and filtered to
leave
cis-2-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]cyclopropanecarboxylic
acid (0.200 g).
[0544] MS (+veES) 232 ((M+H).sup.+)
Step ii:
Cis-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1-
,2,4-oxadiazol-5-yl]cyclopropanecarboxamide
[0545]
Cis-2-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]cyclopropanecarboxylic
acid (0.139 g) and
[0546] N,N'-carbonyldiimidazole (0.110 g) in dichloromethane was
stirred under nitrogen for 1 hour.
1-(3,4-dichlorobenzyl)-4-piperidinamine hydrochloride (0.198 g),
and triethylamine (0.121 g) was then added and allowed to stir for
24 hours under nitrogen. Saturated sodium hydrogen carbonate was
added to the reaction with the resulting solution being extracted
three times with dichloromethane. The pooled organic phases were
washed once with water, once with brine, dried over magnesium
sulfate, filtered and the solvent removed under reduced pressure to
leave an oil. This oil was purified by RPHPLC (75%-5%, 0.1%
ammonium acetate/acetonitrile). The solvent was removed under
reduced pressure to leave
Cis-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1,2-
,4-oxadiazo-5-yl]cyclopropanecarboxamide (0.054 g) as a white
solid.
[0547] MS (+veES) 472 ((M+H).sup.+)
[0548] .sup.1H NMR (DMSO) .delta. 8.74-8.73(1H,m); 8.26-8.24(1H,m);
8.03-7.98(2H,m); 7.59-7.55(2H,m); 7.51(1H,s); 7.27-7.25(1H,m);
3.44-3.37(3H,m); 2.67-2.63(3H,m); 2.27-2.21(1H,m); 2.00-1.89(2H,m);
1.66-1.65(2H,m); 1.59-1.56(1H,m); 1.48-1.43(1H,m);
1.37-1.32(2H,m).
EXAMPLE 356
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1H-1,2,4-triaz-
ol-5-yl]propanamide
Step i: 3-[3-(2-Pyridinyl)-1H-1,2,4-triazol-5-yl]propanoic acid
[0549] 2-Pyridinecarbohydrazonamide (0.136 g) and
dihydro-2,5-furandione (0.100 g) in 1 ml of dimethylacetamide was
heated for 10 times 30 seconds in a CEM MARS 5 microwave at 100% of
300 W under nitrogen to leave
3-[3-(2-pyridinyl)-1H-1,2,4-triazol-5-yl]propanoic acid in 1 ml of
dimethylacetamide.
[0550] MS (-veES) 217 ((M-H).sup.+)
Step ii:
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1H-1,-
2,4-triazol-5-yl]propanamide
[0551] 3-[3-(2-Pyridinyl)-1H-1,2,4-triazol-5-yl]propanoic acid
(0.218 g in 1 ml dimethylacetamide) and N,N'-carbonyldiimidazole
(0.250 g) in dichloromethane was stirred under nitrogen for 30
minutes. 1-(3,4-Dichlorobenzyl)-4-piperidinamine hydrochloride
(0.316 g), and triethylamine (0.218 g) was then added and allowed
to stir for 2 hours under nitrogen. 1M sodium hydroxide was added
to the reaction with the resulting solution being washed three
times with dichloromethane. The aqueous phase was acidified with
glacial acetic acid, with the water/acetic acid being removed under
reduced pressure. Water was then added and extracted three times
with dichloromethane. The pooled organic phases were extracted once
with water and the water removed under reduced pressure to leave a
white solid. This was then triturated with diethyl
ether/dichloromethane, filtered and was purified by RPHPLC (75%-5%,
0.1% ammonium acetate/acetonitrile), solvent removed to leave
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1H-1,2,4-tria-
zol-5-yl]propanamide (0.02 g).
[0552] MS (+veES) 459 ((M+H).sup.+)
[0553] .sup.1H NMR (DMSO) .delta. 8.66-8.65(1H,m); 8.03-8.01(1H,m);
7.95-7.91(1H,m); 7.83-7.81(1H,m); 7.58-7.56(1H,m); 7.52(1H,m);
7.47-7.44(1H,m); 7.29-7.27(1H,m); 3.55-3.50(1H,m); 3.43(2H,s);
2.93-2.89(2H,m); 2.68-2.67(2H,m); 2.55-2.49(2H,m); 2.04-1.98(2H,m);
1.70-1.68(2H,m); 1.42-132(2H,m).
EXAMPLE 357
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(3-phenyl-1H-1,2,4-triazol-5-yl-
)acetamide
[0554] (3-Phenyl-1H-1,2,4-triazol-5-yl)acetic acid (0.020 g) and
N,N'-carbonyl diimidazole (0.016 g) in dichloromethane was stirred
under nitrogen for 30 minutes.
1-(3,4-Dichlorobenzyl)-4-piperidinamine hydrochloride (0.031 g) and
triethylamine (0.036 g) was then added and allowed to stir for 1
hour under nitrogen. Saturated sodium hydrogen carbonate was added
to the reaction with the resulting solution being extracted three
times with dichloromethane. The pooled organic phases were washed
once with water, once with brine, dried over magnesium sulfate,
filtered and the solvent removed under reduced pressure to a white
solid. This was purified by RPHPLC (75%-5%, 0.1% ammonium
acetate/acetonitrile). Saturated sodium hydrogen carbonate was
added to the pooled collected fractions with the resulting solution
being extracted three times with dichloromethane. The pooled
organic phases were washed once with water, once with brine, dried
over magnesium sulfate, filtered and the solvent removed under
reduced pressure to leave
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-(3-phenyl-1H-1,2,4-triazol-5-y-
l)acetamide (0.031 g).
[0555] MS (+veES) 444 ((M+H).sup.+)
[0556] .sup.1H NMR (DMSO) .delta. 8.18-8.15(1H,m); 7.98-7.95(2H,m);
7.59-7.54(2H,m); 7.49-7.41(3H,m); 7.31-7.29(1H,m); 3.63(2H,s);
3.57-3.47(1H,m); 3.45(2H,s); 2.74-2.70(2H,m); 2.08-2.01(2H,m);
1.77-1.74(2H,m); 1.48-1.38(2H,m).
EXAMPLE 358
N-1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-(5-phenyl-1,3,4-oxadiazol-2-yl)a-
cetamide acetate
[0557] 3-(5-Phenyl-1,3,4-oxadiazol-2-yl)propanoic acid (0.175 g)
and N,N'-carbonyldiimidazole (0.148 g) in dichloromethane was
stirred under nitrogen for 30 minutes.
1-(3,4-Dichlorobenzyl)-4-piperidinamine hydrochloride (0.263 g),
and triethylamine (0.126 g) was then added and allowed to stir for
2 hours under nitrogen. Saturated sodium hydrogen carbonate was
added to the reaction, with the resulting solution being extracted
three times with dichloromethane. The pooled organic phases were
washed once with water, once with brine, dried over magnesium
sulfate, filtered and the solvent removed under reduced pressure to
leave a cream solid. This solid was purified by chromatography
using 2.5% ethanol/dichloromethane. The solvent was removed under
reduced pressure and was purified by RPHPLC (75%-5%, 0.1% ammonium
acetate/acetonitrile), followed by 1 ml of glacial acetic acid
being added and the solvent removed under reduced pressure to leave
N-[1-(3,4-dichlorobenzyl)-4-piper-
idinyl]-2-(5-phenyl-1,3,4-oxadiazol-2-yl)acetamide acetate (0.024
g).
[0558] MS (+veES) 445 ((M+H).sup.+)
[0559] .sup.1H NMR (DMSO) .delta. 8.31-8.29(1H,m); 7.98-7.96(2H,m);
7.66-7.54(5H,m); 7.31-7.29(1H,m); 3.92(2H,s); 3.57-3.56(1H,m);
3.46(2H,s); 2.74-2.71(2H,m); 2.07-2.02(2H,m); 1.78-1.75(2H,m);
1.47-1.39(2H,m).
EXAMPLE 359
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1,2,4-oxadiazo-
l-5-yl]acetamide
Step i: Lithium[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]acetate
[0560] 2-(5-Methyl-1,2,4-oxadiazol-3-yl)pyridine (0.150 g) was
stirred at -78.degree. C. in dry tetrahydrofuran under nitrogen.
(1.6M) n-butyl lithium (0.757 ml) was added dropwise so as to
maintain the temperature at -78.degree. C. After 30 minutes carbon
dioxide was passed through the solution and the reaction was
allowed to return to room temperature. Once the reaction had
reached room temperature, water (1 ml) was added and all solvents
were removed under reduced pressure to leave a yellow solid. This
solid was triturated with ethyl acetate and filtered to leave a
pale yellow solid (0.150 g).
[0561] .sup.1H NMR (DMSO+D.sub.2O) .delta. 8.75-8.73(1H,m);
8.12-8.00(2H,m); 7.65-7.61 (1H,m); 3.77(2H,s).
Step ii:
N-[1-(3,4-Dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1,2,4-
-oxadiazol-5-yl]acetamide
[0562] Lithium[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]acetate (0.140
g), 1-(3,4-dichlorobenzyl)-4-piperidinamine (0.170 g), PyBroP.TM.
(0.400 g) were stirred under nitrogen in dimethylformamide (15 ml).
N,N-Diisopropylethylamine (0.171 g) was added and left to stir for
2 hours. 1M sodium hydroxide was added to the reaction, with the
resulting solution being extracted three times with
dichloromethane. The pooled organic phases were washed once with
water, once with brine, dried over magnesium sulfate, filtered and
the solvent removed under reduced pressure to leave product plus
dimethylformamide. Water was added which resulted in precipitation
of the product. The product was filtered and was purified by RPHPLC
(75%-5%, 0.1% ammonium acetate/acetonitrile). After removal of the
solvent under reduced pressure the resulting white solid was
triturated with diethyl ether, filtered and dried to leave
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[3-(2-pyridinyl)-1,2,4-oxadiaz-
ol-5-yl]acetamide (0.067 g).
[0563] m.p. 145.degree. C.
[0564] MS (+veES) 446 ((M+H).sup.+)
[0565] .sup.1H NMR (DMSO) .delta. 8.77-8.75(1H,m); 8.37-8.35(1H,m);
8.07-8.00(2H,m); 7.62-7.54(3H,m); 7.31-7.30(1H,m); 4.02(2H,s);
3.60-3.55(1H,m); 3.46(2H,s); 2.74-2.67(2H,m); 2.08-2.03(2H,m);
1.78-1.76(2H,m); 1.48-1.39(2H,m).
EXAMPLE 360
N-[1-(4-Bromobenzyl)-4-piperidinyl]-2-(4-fluorophenyl)acetamide
[0566] 2-(4-Fluorophenyl)-N-(4-piperidinyl)acetamide (WO97/36871;
1.00 g), 1-bromo-4-(bromomethyl)benzene (1.06 g) and potassium
carbonate (0.877 g) in dimethylformamide (15 ml) were heated to
70.degree. C., under nitrogen for 1 hour. Water was added to the
reaction, with the resulting solution being extracted three times
with dichloromethane. The pooled organic phases were washed once
with water, once with brine, dried over magnesium sulfate, filtered
and the solvent removed under reduced pressure to leave a cream
solid. This solid was triturated with diethyl ether, filtered and
recrystallised from ethanol/water to give white crystalline needles
of
N-[1-(4-bromobenzyl)-4-piperidinyl]-2-(4-fluorophenyl)acetamide.
[0567] m.p. 144.degree. C.
[0568] MS (+veES) 407 ((M+H).sup.+)
[0569] .sup.1H NMR (DMSO) .delta. 7.99-7.98(1H,m); 7.51-7.49(2H,m);
7.28-7.24(4H,m); 7.12-7.06(2H,m); 3.51-3.46(1H,n); 3.41(2H,s);
3.36(2H,s); 2.72-2.69(2H,m); 2.01-1.96(2H,m); 1.70-1.68(2H,m);
1.42-1.34(2H,m).
EXAMPLE 361
2-(4-Fluorophenyl)-N-[1-(2-quinolinylmethyl)-4-piperidinyl]acetamide
[0570] 2-(4-Fluorophenyl)-N-(4-piperidinyl)acetamide (WO97/36871;
0.05 g), 2-quinolinecarbaldehyde (0.033 g) and sodium
triacetoxyborohydride (0.067 g) in dichloroethane (3 ml) were
stirred under nitrogen for 24 hours. Saturated sodium hydrogen
carbonate was added to the reaction, with the resulting solution
being extracted three times with dichloromethane. The pooled
organic phases were washed once with water, once with brine, dried
over magnesium sulfate, filtered and the solvent removed under
reduced pressure, triturated with diethyl ether/ethyl acetate and
filtered lo leave
2-(4-fluorophenyl)-N-[1-(2-quinolinylmethyl)-4-piperidinyl]acetamid-
e (0.020 g).
[0571] MS (+veES) 378 ((M+H).sup.+)
[0572] .sup.1H NMR (DMSO) .delta. 8.34-8.31(1H,m); 8.02-7.94(3H,m);
7.75-7.71(1H,m); 7.63-7.55(2H,m); 7.28-7.25(2H,m); 7.13-7.08(2H,m);
3.74(2H,s); 3.57-3.50(1H,m); 3.30(2H,s); 2.79-2.76(2H,m);
2.16-2.11(2H,m); 1.73-1.70(2H,m); 1.48-1.39(2H,m).
EXAMPLE 362
N-[1-(3-Chloro-4-fluorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1,2,4-oxa-
diazol-5-yl]propanamide
[0573] 3-[3-(2-Pyridinyl)-1,2,4-oxadiazol-5-yl]propanoic acid
(0.218 g) and N,N'-carbonyldiimidazole (0.194 g) were stirred in
dichloromethane (10 ml) under nitrogen for 1 hour.
1-(3-Chloro-4-fluorobenzyl)-4-piperidi- namine (JP 59101483; 0.242
g) was then added and left to stir for 24 hours. Saturated sodium
hydrogen carbonate was added to the reaction, with the resulting
solution being extracted three times with dichloromethane. The
pooled organic phases were washed once with water, once with brine,
dried over magnesium sulfate, filtered and the solvent removed
under reduced pressure, triturated with ethyl acetate/ethanol and
filtered to leave
N-[1-(3-chloro-4-fluorobenzyl)-4-piperidinyl]-3-[3-(2-p-
yridinyl)-1,2,4-oxadiazol-5-yl]propanamide.
[0574] m.p. 150.degree. C.
[0575] MS (+veAPC) 444 ((M+H).sup.+)
[0576] .sup.1H NMR (DMSO) .delta. 8.75-8.74(1H,m); 8.05-7.99(2H,m);
7.95-7.93(1H, m); 7.61-7.58(1H,m); 7.48-7.45(1H,m);
7.37-7.30(1H,m); 7.30-7.26(1H,m); 3.53-3.51(1H,m); 3.42(2H,s);
3.21-3.17(2H,m); 2.71-2.66(4H,m); 2.02-1.96(2H,m); 1.70-1.67(2H,m);
1.42-1.33(2H,m).
EXAMPLE 363
N-[1-(4-Chloro-3-fluorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-1,2,4-oxa-
diazol-5-yl]propanamide
Step i: tert-Butyl
1-(4-chloro-3-fluorobenzyl)-4-piperidinylcarbamate
[0577] 4-Chloro-3-fluorobenzaldehyde (0.793 g) and tert-butyl
4-piperidinylcarbamate (1.00 g) were stirred under nitrogen in
dried tetrahydrofuran (25 ml). Sodium triacetoxyborohydride (1.266
g) was then added and left for 24 hours. Saturated sodium hydrogen
carbonate was added to the reaction, with the resulting solution
being extracted three times with dichloromethane. The pooled
organic phases were washed once with water, once with brine, dried
over magnesium sulfate, filtered and the solvent removed under
reduced pressure to leave tert-butyl
1-(4-chloro-3-fluorobenzyl)-4-piperidinylcarbamate (1.80 g) as a
white solid.
[0578] MS (+veAPC) 343 ((M+H).sup.+)
Step i: 1-(4-Chloro-3-fluorobenzyl)-4-piperidinamine
[0579] tert-Butyl
1-(4-chloro-3-fluorobenzyl)-4-piperidinylcarbamate (1.80 g) in
dichloromethane (20 ml) was stirred under nitrogen. Trifluoroacetic
acid (5 ml) was then added dropwise and the reaction was left to
stir for 2 hours. 1M sodium hydroxide was added to the reaction
until basic, with the resulting solution being extracted three
times with dichloromethane. The pooled organic phases were washed
once with water, once with brine, dried over magnesium sulfate,
filtered and the solvent removed under reduced pressure. Product
purified by chromatography (5% ethanol/dichloromethane to 10%
ethanol/dichloromethane) and solvent runoved under reduced pressure
to leave an oil which crystallised over the period of 48 hours. The
resulting solid was triturated with diethyl ether and filtered to
leave 1-(4-chloro-3-fluorobenzyl)-4-piperidinamine (0.500 g) as a
white solid.
Step iii:
N-[1-(4-Chloro-3-fluorobenzyl)-4-piperidinyl]-3-[3-(2-pyridinyl)-
-1,2,4-oxadiazol-5-yl]propanamide
[0580] 3-[3-(2-Pyridinyl)-1,2,4-oxadiazol-5-yl]propanoic acid
(0.136 g) and N,N'-carbonyldiimidazole (0.114 g) were stirred in
dichloromethane (10 ml) under nitrogen for 1 hour.
1-(4-Chloro-3-fluorobenzyl)-4-piperidi- namine (0.150 g) was then
added and left to stir for 2 hours. Saturated sodium hydrogen
carbonate was added to the reaction, with the resulting solution
being extracted three times with dichloromethane. The pooled
organic phases were washed once with water, once with brine, dried
over magnesium sulfate, filtered and the solvent removed under
reduced pressure to leave an oil. This was triturated with diethyl
ether which caused product the to crystallise. After filtration,
the product was washed with diethyl ether and dried to
N-[1-(4-chloro-3-fluorobenzyl)-4-p-
iperidinyl]-3-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]propanamide.
[0581] m.p. 132.degree. C.
[0582] MS (+veES) 444 ((M+H).sup.+)
[0583] .sup.1H NMR (DMSO) .delta. 8.76-8.74(1H,m); 8.05-7.99(2H,m);
7.95-7.94(1H,m); 7.61-7.58(1H,m); 7.54-7.50(1H,m); 7.32-7.28(1H,m);
7.16-7.14(1H,m); 3.55-3.47(1H,m); 3.44(2H,s); 3.21-3.17(2H,m);
2.71-2.66(4H,m); 2.03-1.97(2H,m); 1.70-1.67(2H,m);
1.42-1.33(2H,m).
EXAMPLE 364
2-(4-Chlorophenoxy)-N-[1-[(3,4-dichlorophenyl)methyl]-piperidin-4-yl]-acet-
amide
[0584] The product from Example 1 step (ii) was dissolved in
dichloromethane (10 ml) containing triethylamine (0.081 g) and the
solution was cooled to 0.degree. C. 4-Chlorophenoxyacetyl chloride
(88 mg) in dichloromethane (3 ml) was added dropwise, the cooling
bath was removed and the resulting solution was stirred for 1 hour.
Ethyl acetate, water and brine were added and the phases were
separated. The organic phase was dried, filtered and evaporated to
give an oil which was purified by reverse phase HPLC (with a
gradient eluent system (25% MeCN/NH.sub.4OAc.sub.aq (0.1%) to 95%
MeCN/NH.sub.4OAc.sub.aq (0.1%)) to give the title compound (0.049
g).
[0585] .sup.1H NMR: (CDCl.sub.3): .delta.]1.51 (2H, ddd), 1.89-1.96
(2H, m), 2.15 (2H, td), 2.77 (2H, d), 3.43 (2H, s), 3.85-3.96 (1H,
m), 4.44 (2H, s), 6.37 (1H, d), 6.85 (2H, dt), 7.14 (1H, dd),
7.26-7.29 (2H, m), 7.37 (1H, d), 7.43 (1H, d)
EXAMPLE 365
N-(1-benzyl-4-piperidinyl)-3-[3-(2-pyridinyl)-1,2,4-oxadiazol-5-yl]propana-
mide
[0586] To a solution of
3-(3-Pyridin-2-yl-[1,2,4]oxadiazol-5-yl)-propionic acid (1 g) in
tetrahydrofuran (5 ml), was added carbonyldiimidazole (0.74 g). The
mixture was stirred for 10 minutes before addition of
1-benzyl-piperidin-4-ylamine (1 ml) in tetrahydrofuran (5 ml). The
reaction mixture was stirred for 15 minutes then partitioned
between ethyl acetate (20 ml) and water (20 ml). The organic layer
was separated, dried (MgSO.sub.4) and solvent removed by
evaporation. Purification by Biotage.RTM. 40S eluting 3% MeOH/0.5%
880 ammonia/dichloromethane gave the title compound (0.93 g).
[0587] MS: ESI 392 (+H)
[0588] .sup.1H NMR: (CDCl.sub.3): .delta. 1.44 (2H, ddd), 1.88 (2H,
d), 2.10 (2H, t), 2.73-2.78 (2H, m), 2.80 (2H, t), 3.33 (2H, t),
3.46 (2H, s), 3.75-3.86 (1H, m), 5.57 (1H, d), 7.23-7.32 (5H, m),
7.42 (1H, ddt), 7.84 (1H, tt), 8.10 (1H, dd), 8.79 (1H, td).
EXAMPLE 366
N-(2-{[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-methyl-amino}-ethyl)-2-(2-f-
luoro-phenyl)-acetamide
Step i: (2-Methylamino-ethyl)-carbamic acid tert-butyl ester
[0589] To a solution of (2-amino-ethyl)-carbamic acid-tert-butyl
ester (5 g) and triethylamine (6.5 ml) in tetrahydrofuran (1000 ml)
at 0.degree. C. was added methyliodide (1.94 ml) dropwise over a
period of 1 hour. The mixture was allowed to warm to ambient
temperature and stirred for 72 hours before removal of solvents by
evaporation. The residue was partitioned between ethyl acetate and
water. The organic layer was separated, dried (MgSO.sub.4) and
solvent removed by evaporation to give the title compound (3.7
g).
[0590] MS: ESI 57((CH.sub.3).sub.4C+), 118
(M+H--(CH.sub.3).sub.4C)
Step ii:
(2-{[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-methyl-amino}-ethyl)-
-carbamic acid tert-butyl ester
[0591] To a solution of dichlorobenzyl-piperidin-4-one (Example 74,
step (i), 4.8 g) and acetic acid (1 ml) in dichloromethane (100 ml)
was added (2-methylamino-ethyl)-carbamic acid tert-butyl ester
(3.26 g) and the mixture was stirred for 5 minutes before addition
of sodium triacetoxyborohydride (7.9 g). The reaction mixture was
stirred for 12 hours before addition of sodium bicarbonate
solution. The mixture was stirred for 1/2 hour and then partitioned
between water and dichloromethane. The organic layer was separated,
dried (MgSO.sub.4) and solvent removed by evaporation. Purification
by Biotage.RTM. 40S eluting 10% MeOH/2%
triethylaniine/dichloromethane gave the title compound (1.7 g).
[0592] MS: ESI 316/318 (+H--(CH.sub.3).sub.4COCO)
[0593] .sup.1H NMR: (CDCl.sub.3): .delta. 1.44 (9H, s), 1.50-1.60
(4H, m), 1.65-1.72 (2H, m), 1.95 (2H, td), 2.23 (3H, s), 2.34 (1H,
tt), 2.88 (2H, d), 3.14-3.20 (2H, m), 3.41 (2H, s), 4.95-5.01 (1H,
m), 7.13-7.15 (1H, m), 7.37 (1H, d), 7.42 (1H, d).
Step iii:
N.sup.1-[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-N.sup.1-methyl--
ethane-1,2-diamine
[0594]
(2-{[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-methyl-amino}-ethyl)-c-
arbamic acid tert-butyl ester (1.7 g) was dissolved in 6M HCl (20
ml) and stirred for 12 hours. The solvent was evaporated and the
residue was azeotroped with toluene and then sodium bicarbonate
solution was added. The mixture was stirred for 10 minutes and the
product was extracted with dichloromethane. The solvent was removed
by evaporation to give the title compound (0.75 g).
[0595] MS: ESI 316/318 (+H)
Step iv:
N-(2-{[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-methyl-amino}-ethy-
l)-2-(2-fluoro-phenyl)-acetamide
[0596] Prepared by the method of Example 359 step (ii) using
N.sup.1-[1-(3,4-Dichloro-benzyl)-piperidin-4-yl]-N.sup.1-methyl-ethane-1,-
2-diamine and 2-fluorophenylacetic acid.
[0597] MS: ESI 452/454 (+H)
[0598] .sup.1H NMR: (CDCl.sub.3): .delta. 2.08-1.94 (2H, m),
2.37-2.33 (2H, m), 2.95 (3H, s), 3.18 (2H, t), 3.41 (2H,
m),3.66-3.78(4H, m), 3.75 (2H, s), 3.84 (1H, m), 4.38 (2H, s),
7.16-7.28 (2H, m), 7.36-7.42 (2H, m), 7.45 (1H, dd), 7.73 (1H, d),
7.72 (1H, d).
EXAMPLE 367
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N-methyl-2-(4-fluorophenyl)acetam-
ide
Step i: [1-(3,4-Dichlorobenzyl)-piperidin-4-yl]-methyl-amine
[0599] To a solution of 1-(3,4-Dichlorobenzyl)-piperidin-4-one (3.1
g) in dichloromethane (50 ml) and acetic acid (0.69 ml) was added
methylamine (6 ml of a 1M solution in tetrahydrofuran). The mixture
was stirred for 5 minutes before the addition of sodium
triacetoxyborohydride (3 g) and the resulting mixture stirred for
72 hours. Sodium bicarbonate solution (100 ml) added and the
mixture stirred vigorously for 5 minutes before extraction of the
product with dichloromethane (2.times.200 ml). The organics were
separated, bulked and dried, (MgSO.sub.4). Purification by
Biotage.RTM. 40S eluting 10% MeOH/0.5% 880 ammonia/dichloromethane
gave the sub-title compound (1.8 g).
[0600] MS: ESI 273/275 (+H)
[0601] .sup.1H NMR: (CDCl.sub.3): .delta. 1.36 (2H, qd), 1.82-1.91
(2H, m), 2.03 (2H, td), 2.36 (1H, tt), 2.43 (3H, s), 2.76-2.83 (2H,
m), 3.43 (2H, s), 7.15 (1H, dd), 737 (1H, d), 7.42 (1H, d).
Step ii:
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-(4-fluorophenyl)acetam-
ide
[0602] To a solution of 4-fluorophenylacetic acid (100 mg) in
tetrahydrofuran (3 ml) was added carbonyldiimidazole (105 mg). The
mixture was stirred for 10 minutes before addition of
[1-(3,4-dichlorobenzyl)-piperidin-4-yl]-methyl-amine (177 mg) in
tetrahydrofuran (2 ml). Stirring was continued for 1 hour then
solvent removed by evaporation. Purification by Biotage.RTM. 40S
eluting 2% MeOH/0.5% 880 ammnonia/dichloromethane gave the title
compound (166 mg).
[0603] MS: ESI 273/275 (M+H)
[0604] .sup.1H NMR: (CDCl.sub.3) .delta. 1.57 (1H, d), 1.69 (1H,
qd), 1.76-1.84 (1H, m), 1.88 (1H, q), 2.10 (2H, td), 2.85-2.90.(1H,
m), 2.85 (3H, s), 3.42 (2H, s), 3.58 (1H, tt), 3.67 (2H, s), 4.51
(1H, tt), 7.00 (2H, t), 7.11-7.15 (1H, m), 7.18-7.23 (2H, m), 7.37
(1H, d), 7.41 (1H, dd).
EXAMPLE 368
N-[1-[(3,4-dichlorophenyl)methyl)-4-piperidinyl]-2-(2-pyrimidinyloxy)-acet-
amide
Step i: Ethyl 2-pyrimidinyloxyacetate
[0605] Ethyl glycolate (1.04 g) was dissolved in tetrahydrofuran
(10 ml) and the solution was cooled to 0.degree. C. Sodium hydride
(60% suspension in oil, 0.43 g) was added and the suspension was
stirred and then sonicated in an ultrasonic bath.
2-Chloropyrimidine (1.14 g) was added and the mixture was sonicated
for a further 110 min. Ammonium chloride solution was added and the
mixture was extracted thrice with ethyl acetate, the organic phases
were washed with brine and dried, filtered and evaporated. The
residue was purified by chromatography eluting with
iso-hexane:ethyl acetate (13:7) to give the subtitle compound (1.40
g) as an oil.
[0606] .sup.1H NMR (299.944 MHz, CDCl.sub.3) .delta. 1.26 (t, J=6.8
Hz, 3H), 4.24 (q, J=7.1 Hz, 2H), 4.93 (s, 2H), 6.98 (t, J=4.8 Hz,
1H), 8.53 (d, J=4.8 Hz, 2H).
Step ii: 2-Pyrimidinyloxyacetic acid
[0607] Ethyl 2-pyrimidinyloxyacetate (1.4 g) was dissolved in
ethanol (10 ml). Sodium hydroxide (2M aq) was added and the mixture
was stirred for 64 h. The solvent was evaporated and the reside was
dissolved in water, filtered and the acidified with concentrated
hydrochloric acid. The resulting precipitate was collected and
dried to give the subtitle compound (0.698 g).
[0608] .sup.1H NMR (399.98 MHz, DMSO) .delta. 4.85 (s, 2H), 7.09
(t, J=4.9 Hz, 1H), 8.56 (d, J=4.8 Hz, 2H).
Step iii:
N-[1-[(3,4-dichlorophenyl)methyl]-4-piperidinyl]-2-(2-pyrimidiny-
loxy)-acetamide
[0609] The title compound was prepared from the product of Example
1 step (ii) (hydrochloride salt, 335 mg) and 2-pyrimidinyloxyacetic
acid (170 mg) using the method of Example 94.
[0610] Yield 140 mg.
[0611] m.p. 120-122.degree. C.
[0612] IH NMR (399.978 MHz, CDCl.sub.3) .delta. 1.50 (q, J=11.6 Hz,
2H), 1.91 (d, J=11.9 Hz, 2H), 2.13 (t, J=11.1 Hz, 2H), 2.77 (d,
J=11.4 Hz, 2H), 3.42 (s, 2H), 3.86-3.95 (m, 1H), 4.87 (s, 2H), 6.49
(d, J=6.9 Hz, 1H), 7.05 (t, J=4.9 Hz, 1H), 7.14 (m, 1H), 7.37 (d,
J=8.3 Hz, 1H), 7.42 (s, 1H), 8.57 (d, J=4.8 Hz, 2H).
EXAMPLE 369
N-[2-[[8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl]amino]et-
hyl]-3-methoxy-benzamide, bis toluene sulfonic acid salt
[0613] 141
Step i:
8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-one
[0614] 2,5-Dimethoxytetrahydrofuran (4.92 g) was stirred in
hydrochloric acid (1M, 25 ml) for 1 hour. 3,4-Dichlorobenzylamine
(5 ml) was added to hydrochloric acid (1M, 15 ml) and the resulting
suspension was added to the first solution. Phosphate buffer
solution (pH 5.5, 250 ml) was added followed by sodium hydroxide
(1.6 g). A solution of acetone dicarboxylic acid (4.77 g) in
phosphate buffer solution (pH 5.5, 90 ml) was added to the mixture
and the solution was stirred. A yellow solid formed and the mixture
was left to stand for 64 h. The aqueous supernatant was decanted
and hydrochloric acid (2.5M) was added to the solid along with
ethyl acetate. The layers were separated and the aqueous phase was
extracted twice with dichloromethane containing a little methanol.
The organic layers were combined and evaporated to give a crude oil
(ca 7 g). A portion of the product (ca 2.5 g) was purified by
chromatography eluting with dichloromethane:methanol (19:1) to give
the subtitle compound (1.62 g) as a yellow oil.
[0615] .sup.1H NMR (299.944 MHz, CDCl.sub.3) .delta. 1.62-1.70 (m,
2H), 2.09-2.15 (m, 2H), 2.23 (d, J=15.9 Hz, 2H), 2.67 (d, J=16.7
Hz, 2H), 3.43-3.49 (m, 2H), 3.68 (s, 2H), 7.26 (d, J=8.7 Hz, 1H),
7.41 (d, J=7.5 Hz, 1H), 7.54 (s, 1H)
Step ii: Carbamic acid,
Endo-[2-[[8-[(3,4-dichlorophenyl)methyl]-8-azabicy-
clo[3.2.1]oct-3-yl]amino]ethyl]-1,1-dimethylethyl ester
[0616]
8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-one (751
mg) and carbamic acid, (2-aminoethyl)-1,1-dimethylethyl ester (520
mg) were dissolved in dichloroethane (23 ml). Sodium
triacetoxyborohydride (697 mg) was added and the suspension was
stirred at room temperature for 20 hours. Dichloromethane was added
and the solution was to washed with sodium bicarbonate solution,
then with water and then with brine. Chromatography of the residue
eluting with ethyl acetate:methanol:triethy- lamine (80:19:1) gave
the subtitle compound (688 mg) as an oil.
[0617] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.45 (s, 9H),
1.52 (d, J=14.4 Hz, 2H), 1.96-2.09 (m, 6H), 2.67 (t, J=5.8 Hz, 2H),
2.88 (t, J=6.4 Hz, 1H), 3.08-3.12 (m, 2H), 3.21 (q, J=5.7 Hz, 2H),
3.48 (s, 2H), 4.80-4.95 (m, 1H), 7.22 (dd, J=8.3, 2.0 Hz, 1H), 7.37
(d, J=7.9 Hz, 1H), 7.49 (d, J=2.0 Hz, 1H)
Step iii:
N-[2-[[8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-y-
l]amino]ethyl]-3-methoxy-benzamide, bis toluene sulfonic acid
salt
[0618] Carbamic acid,
[2-[[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2-
.1]oct-3-yl]amino]ethyl]-, 1,1-dimethylethyl ester (337 mg) was
dissolved in dichloromethane (3 ml) and trifluoroacetic acid (3 ml)
was added. The resulting solution was stirred for 1 hour then the
volatiles were evaporated. The residue was dissolved in
dichloromethane (3 ml) and triethylamine (1 ml) was added followed
by 3-methoxybenzoyl chloride (120 .mu.l). The solution was stirred
overnight. The solvent was evaporated and the residue was purified
by RPHPLC (gradient ammonium acetate 1% aqueous: acetonitrile (25%
acetonitrile to 95% acetonitrile)). Excess tosic acid in ether was
added to the residue and the resultant salt was recystallised from
a mixture of ethyl acetate--ethanol with a little cyclohexane to
give the title compound (77 mg).
[0619] m.p. 180-182.5.degree. C.
[0620] .sup.1H NMR (399.98 MHz, DMSO) .delta. 2.10-2.24 (m, 4H),
2.29 (s, 6H), 2.39-2.47 (m, 4H), 3.21-3.28 (m, 2H), 3.52-3.57 (m,
1H), 3.57-3.63 (m, 2H), 3.80 (s, 3H), 3.85-3.91 (m, 2H), 4.21 (d,
J=5.4 Hz, 2H), 7.11 (d, J=9.4 Hz, 4H), 7.13-7.18 (m, 1H), 7.38-7.45
(m, 3H), 7.48 (d, J=7.9 Hz, 4H), 7.56 (d, J=6.7 Hz, 1H), 7.78 (d,
J=8.2 Hz, 1H), 7.84-7.90 (m, 1H), 8.38-8.52 (m, 2H), 8.81-8.87 (m,
1H), 9.44-9.51 (m, 1H).
EXAMPLE 370
Endo-N-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl]-3-(2-p-
yridinyl)-1,2,4-oxadiazole-5-propanamide hydrochloride
Step i:
Endo-8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-ami-
ne
[0621]
8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-one (350
mg) was dissolved in dry methanol (12 ml) and ammonium acetate (1
g) was added. The mixture was stirred to get partial solution and
then sodium cyanoborohydride (106 mg) was added. The mixture was
heated under reflux for 150 minutes, then allowed to cool to room
temperature. The methanol was evaporated, the residue was
partitioned between sodium hydroxide and dichloromethane, and the
aqueous phase was extracted twice with dichloromethane. The organic
phases were combined, dried, filtered and evaporated to give the
subtitle compound.
[0622] [M+H].sup.+ (ES+) 285
Step ii:
Endo-N-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-y-
l]-3-(2-pyridinyl)-1,2,4-oxadiazole-5-propanamide hydrochloride
[0623] 3-(2-Pyridinyl)-1,2,4-oxadiazole-5-propanoic acid (305 mg)
was suspended in dichloromethane (6 ml) and oxalyl chloride (0.5
ml) was added. The mixture was stirred overnight. Toluene (1 ml)
was added to the solution, the volatiles were evaporated, then the
residue was redissolved in dichloromethane (2 ml).
Endo-8-[(3,4-dichlorophenyl)methyl]-8-azabicyc-
lo[3.2.1]octan-3-amine (all from step(i)) was dissolved in
dichloromethane (4 ml) containing triethylamine (0.5 ml) and then
cooled in an ice bath. The acid chloride solution was added to the
amine and the mixture was stirred for 1 hour. Water was added to
the reaction mixture and the phases were separated. The aqueous
phase was extracted twice with dichloromethane, the organic phases
were dried, filtered and evaporated. The residue was purified by
RPHPLC (gradient ammonium acetate 1% aqueous:acetonitrile (25%
acetonitrile to 95% acetonitrile)). The product was suspended in
ether and the ethereal hydrochloric acid was added, the suspension
was stirred and then the diethyl ether was evaporated. The residue
was dissolved in hot ethyl acetate containing ethanol and
crystallisation was induced by adding iso-hexane to give the title
compound (47 mg).
[0624] .sup.1H NMR (399.98 MHz, DMSO) .delta. 1.99-1.90 (m, 2H),
2.41-2.20 (m, 6H), 2.77 (t, J=6.8 Hz, 2H), 3.23 (t, J=6.9 Hz, 2H),
3.81-3.72(m, 3H), 4.15 (d, J=6.2 Hz, 2H), 7.63-7.58 (m, 1H), 7.67
(dd, J=7.6, 2.3 Hz, 2H), 7.76 (d, J=9.3 Hz, 1H), 8.06-7.99 (m, 3H),
8.11 (d, J=4.1 Hz, 1H), 8.75 (d, J=4.6 Hz, 1H), 10.13 (t, J=5.6 Hz,
1H).
EXAMPLE 371
2-[4-(acetylamino)phenoxy]-N-[1-[(3,4-dichlorophenyl)methyl]-4-piperidinyl-
]-acetamide
Step i: Methyl (4-acetaminophenoxy)acetate
[0625] 4-Acetaminophenol (1.51 g), potassium carbonate (1.38 g) and
methyl bromoacetate (1.0 ml) were combined in acetone (40 ml) and
heated to reflux for 5 hours. The mixture was allowed to cool to
room temperature, filtered and evaporated. The residue was
dissolved in ethyl acetate, washed with water and then with brine
then dried, filtered and evaporated to give the subtitle compound
(232 g).
[0626] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 2.16 (s, 3H),
3.80 (s, 3H), 4.62 (s, 2H), 6.87 (d, J=9.1 Hz, 2H), 7.07 (br s,
1H), 7.40 (d, J=9.0 Hz, 2H)
Step ii: (4-Acetaminophenoxy)acetic acid
[0627] Methyl(4-acetaminophenoxy)acetate was hydrolysed following
the method of Example 368 step (ii) to give the subtitle compound
(1.85 g).
[0628] .sup.1H NMR (399.98 MHz, DMSO) .delta. 2.00 (s, 3H), 4.61
(s, 2H), 6.84 (d, J=9.0 Hz, 2H), 7.46 (d, J=9.0 Hz, 2H), 9.80.(s,
1H).
Step iii:
2-[4-(acetylamino)phenoxy]-N-[1-[(3,4-dichlorophenyl)methyl]-4-p-
iperidinyl]-acetamide
[0629] The title compound was prepared from the product of Example
1 step (ii) (free base, 281 mg) and (4-acetaminophenoxy)acetic acid
(229 mg) using a method hereinbefore described (yield 40 mg).
[0630] m.p. 177-178.5.degree. C.
[0631] .sup.1H NMR (299.946 MHz, DMSO) .delta. 1.51 (qd, J=10.5,
3.7 Hz, 2H), 1.72-1.63 (m, 2H), 2.00 (s, 3H), 2.05 (t, J=3.7 Hz,
2H), 2.77-2.68 (m, 2H), 3.45 (s, 2H), 3.70-3.57 (m, 1H), 4.39 (s,
2H), 6.88 (d, J=9.0 Hz, 2H), 7.29 (dd, J=8.1, 1.7 Hz, 1H), 7.47 (d,
J=8.8 Hz, 2H), 7.54 (d, J=1.5 Hz, 1H), 7.58 (d, J=8.1 Hz, 1H), 7.89
(d, J=8.1 Hz, 1H), 9.79 (s, 1H).
EXAMPLE 372
N-[1-[(3,4-dichlorophenyl)methyl-4-piperidinyl]-4-hydroxy-benzeneacetamide
[0632] The title compound was prepared from the product of Example
1 step (ii) (free base, 172 mg) and 4-hydroxyphenylacetic acid (135
mg) using a method hereinbefore described (yield 57 mg).
[0633] m.p. 72-97.degree. C.
[0634] .sup.1H NMR (399.98 MHz, DMSO) .delta. 1.37 (q, J=7.0 Hz,
2H), 1.69 (d, J=11.3 Hz, 2H), 2.02 (t, J=5.3 Hz, 2H), 2.71 (d,
J=11.3 Hz, 2H), 3.23 (s, 2H), 3.44 (s, 2H), 3.55-3.42 (m, 1H), 6.66
(d, J=8.5 Hz, 2H), 7.02 (d, J=8.5 Hz, 2H), 7.29 (d, J=8.2 Hz, 1H),
7.53 (s, 1H), 7.58 (d, J=8.2 Hz, 1H), 7.87 (d, J=7.9 Hz, 1H), 9.18
(s, 1H).
EXAMPLE 373
Exo-N-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl]-3-(2-py-
ridinyl)-1,2,4-oxadiazole-5-propanamide
Step i:
Endo-8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-ol
[0635]
8-[(3,4-Dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-one (330
mg) was dissolved in tetrahydrofuran (5 ml) and cooled to 0.degree.
C. Lithium tris(3-ethylpentyl-3-oxy)aluminohydride solution (0.5M,
2.5 ml) was added dropwise and the mixture was allowed to attain
room temperature overnight. Sodium sulfite decahydrate (ca 2 g) was
added and the suspension was stirred for 1 hour. The reaction
mixture was diluted with ethyl acetate, filtered through kieselguhr
and evaporated. The residue was purified by chromatography eluting
with dichloromethane:methanol (9:1) to give the subtitle compound
161 mg.
[0636] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.59 (d, J=8.1
Hz, 2H), 1.64 (t, J=11.4 Hz, 2H), 1.86-1.81 (m, 2H), 2.00-1.97 (m,
2H), 3.21-3.18 (m, 2H), 3.55 (s, 2H), 3.95 (septet, J=5.6 Hz, 1H),
7.21 (d, J=8.2 Hz, 1H), 7.37 (d, J=7.4 Hz, 1H), 7.50 (s, 1H).
Step ii:
Exo-2-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl-
]-1H-isoindole-1,3(2H)dione
[0637]
Endo-8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-ol
(556 mg), phthalimide (321 mg) and polymer bound triphenylphosphine
(821 mg) were combined in tetrahydrofuran (10 ml).
Diethylazodicaboxylate (330 .mu.l) was added and the mixture was
stirred gently overnight Additional phosphine (0.5 g) and
diethylazodicaboxylate (200 .mu.l) were added and the mixture was
stirred for an additional 5 days. The reaction mixture was diluted
with ethyl acetate and filtered; the residue was washed with ethyl
acetate and methanol. The filtrate was evaporated, and
chromatographed eluting with 9:1 ethyl acetate:methanol. RPHPLC of
the product (gradient ammonium acetate 1% aqueous: acetonitrile
(25% acetonitrile to 100% acetonitrile)) gave the subtitle compound
(90 mg).
[0638] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.47-1.39 (m,
2H), 1.78 (d, J=7.7 Hz, 2H), 2.14-2.02 (m, 2H), 2.64 (t, J=11.8 Hz,
2H), 3.36-3.25 (m, 2H), 3.92-3.81 (m, 2H), 4.56 (septet, J=6.1 Hz,
1H), 7.41-7.32 (m, 2H), 7.59-7.55 (m, 1H), 7.74-7.69 (m, 2H),
7.86-7.82 (m, 2H).
Step iii:
Exo-8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]octan-3-am-
ine
[0639]
Exo-2-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl]--
1H-isoindole-1,3(2H)-dione (90 mg) was dissolved in ethanol (6 ml)
containing dichloromethane (3 ml); hydrazine hydrate (0.2 ml) was
added and the resulting solution was stirred at room temperature
for 26 hours. The suspension was filtered and the filtrate was
evaporated to give the subtitle compound (55 mg).
[0640] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.51-1.43 (m,
2H), 1.59 (q, J=4.9 Hz, 2H), 1.75-1.67 (m, 2H), 2.00-1.94 (m, 2H),
3.02-2.92 (m, 1H), 3.18-3.12 (m, 2H), 3.50 (s, 3H), 7.21 (d, J=8.2
Hz, 1H), 7.37 (d, J=8.2 Hz, 1H), 7.50 (s, 1H).
Step iv:
Exo-N-[8-[(3,4-dichlorophenyl)methyl]-8-azabicyclo[3.2.1]oct-3-yl-
]-3-(2-pyridinyl)-1,2,4-oxadiazole-5-propanamide
[0641] Prepared following the method of Example 370 step (iii) but
without salt formation to give the title compound (15 mg).
[0642] m.p. 177.5-178.degree. C.
[0643] .sup.1H NMR (299.946 MHz, DMSO): .delta. 1.63-1.43 (m, 6H),
1.99-1.90 (m, 2H), 2.64 (t, J=7.1 Hz, 2H), 3.11-3.06 (m, 2H), 3.18
(t, J=6.2 Hz, 2H), 3.49 (s, 2H), 3.97-3.83 (m, 1H), 7.32 (dd,
J=8.3, 1.9 Hz, 1H), 7.62-7.56 (m, 3H), 7.87 (d, J=8.1 Hz, 1H),
8.06-7.97 (m, 2H), 8.75 (dt, J=3.7, 0.8 Hz, 1H).
EXAMPLE 374
(R)
N-[1-[1-(4-bromophenyl)ethyl]-4-piperidinyl]-3-(2-pyridinyl)-1,2,4-oxa-
diazole-5-propanamide
Step i: (R)-1-[1-(4-Bromophenyl)ethyl]-4-piperidinone
[0644] (R)-(4-Bromophenyl)ethylamine (1.01 g) and potassium
carbonate (1.45 g) were dissolved in a mixture of ethanol (13 ml)
and water (6 ml) and then heated to a vigorous reflex. A solution
of 4-hydroxy-4-methoxy-1,1-dimethyl-piperidinium iodide (J. Chem.
Soc. Perkin Trans. 2, (1984) 1647) (1.47 g) in warm water (6 ml)
was added dropwise over 40 minutes; reflux was maintained for a
further 12 hours, then the reaction was allowed to cool to room
temperature. The mixture was evaporated and ethyl acetate and water
were added and the phases were separated. The aqueous phase was
extracted twice with ethyl acetate, the organic layer was washed
with brine, dried, filtered and evaporated. Chromatography of the
residue eluting with iso-hexane:ethyl acetate (3:2) gave the
subtitle compound (804 mg).
[0645] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 2.66-2.80 (m,
4H), 1.38 (d, J=6.9 Hz, 3H), 2.42 (t, J=6.2 Hz, 4H), 3.58 (q, J=6.7
Hz, 1H), 7.24 (d, J=8.5 Hz, 2H), 7.46 (d, J=9.0 Hz, 4H).
Step ii: (R)-1-[1-(4-Bromophenyl)ethyl]-4-piperidinamine
[0646] Prepared following the general method of Example 370 step
(i) (R)-1-[1-(4-bromophenyl)ethyl]-4-piperidinone (420 mg) ammonium
acetate (0.80 g) and sodium cyanoborohydride (120 mg) to give the
subtitle compound (449 mg).
[0647] .sup.1H NMR (399.978 MHz, CDCl.sub.3) .delta. 1.33 (d, J=6.9
Hz, 3H), 1.43-1.26 (m, 2H), 1.73 (d, J=12.3 Hz, 1H), 1.81 (d,
J=12.6 Hz, 1H), 2.03-1.90 (m, 2H), 2.60 (tt, J=10.6, 5.1 Hz, 1H),
2.71 (d, J=13.6 Hz, 1H), 2.94 (d, J=11.3 Hz, 1H), 3.37 (q, J=6.7
Hz, 1H), 7.18 (d, J=8.5 Hz, 2H), 7.43 (d, J=8.2 Hz, 2H)
Step iii: (R)
N-[1-[1-(4-bromophenyl)ethyl]-4-piperidinyl]-3-(2-pyridinyl)-
-1,2,4-oxadiazole-5-propanamide
[0648] Prepared following a method as hereinbefore described using
(R)-1-[1-(4-bromophenyl)ethyl]-4-piperidinamine (449 mg),
3-(2-pyridinyl)-1,2,4-oxadiazole-5-propanoic acid (0.3 1 g),
1-hydroxybenzotriazole (0.20 g), 4-(N,N-dimethylamino)-pyridine
(0.13 g) and 1-ethyl-3-[3-dimethylamino)-propyl]carbodiimide
hydrochloride (0.30 g) to give the title compound (40 mg).
[0649] m.p. 153-155.degree. C.
[0650] .sup.1H NMR (399.98 MHz, DMSO) .delta. 1.23 (d, J=6.7 Hz,
3H), 1.40-1.26 (m, 2H), 1.66-1.61 (m, 1H), 1.73-1.67 (m, 1H),
1.97-1.86 (m, 2H), 2.64-2.59 (m, 1H), 2.66 (t, J=7.2 Hz, 2H),
2.84-2.79 (m, 1H), 3.18 (t, J=7.2 Hz, 2H), 3.42 (q, J=6.4 Hz, 1H),
3.48-3.39 (m, 1H), 7.25 (d, J=8.5 Hz, 2H), 7.49 (d, J=8.5 Hz, 2H),
7.59 (ddd, J=6.7, 4.6, 2.1 Hz, 1H), 7.91 (d, J=7.4 Hz, 1H),
8.04-7.99 (m, 2H), 8.75 (dt, J=4.4, 1.4 Hz, 1H).
EXAMPLE 375
(S)
N-[1-[1-(4-bromophenyl)ethyl]-4-piperidinyl]-3-(2-pyridinyl)-1,2,4-oxa-
diazole-5-propanamide
[0651] Prepared following an analogous series of steps to example
374 but using (S)-(4-bromophenyl)ethylamine to give the title
compound.
[0652] m.p. 141.5-143.degree. C.
[0653] .alpha..sub.D 29.55.degree. (c=0.13, methanol 21.degree.
C.)
[0654] .sup.1H NMR (299.946 MHz, DMSO) .delta. 1.23 (d, J=6.7 Hz,
3H), 1.26-1.41 (m, 2H), 1.64 (t, J=8.1 Hz, 2H), 1.92 (q, J=11.2 Hz,
2H), 2.58-2.67 (m, 1H), 2.67 (t, J=7.2 Hz, 2H), 2.78-2.85 (m, 1H),
3.18 (t, J=7.1 Hz, 2H), 3.37-3.46 (m, 1H), 3.42 (q, J=6.7 Hz, 1H),
7.25 (d, J=6.7 Hz, 2H), 7.49 (d, J=8.5 Hz, 2H), 7.57-7.62 (m, 1H),
7.91 (d, J=7.7 Hz, 1), 7.98-8.05 (m, 2H), 8.75 (d, J=7.5 Hz,
1H).
EXAMPLE 385
1-[3,4-Dichlorobenzyl]-N-[3-(3-pyridinyl)propyl]-4-piperidinamine
[0655] 142
[0656] The title compound was prepared from
1-(3,4-dichlorobenzyl)piperidi- ne-4-amine (free base 187 mg),
3-(3-pyridinyl)propanal (125 mg), sodium triacetoxyborohydride (70
mg), and 0.02 ml acetic acid, stirred together for 2 hrs in
dichloromethane (10 ml). Water was added, the mixture neutralised
with sodium bicarbonate and the organic phase separate, dried and
chromatographed on silica with ethyl acetate/methanol (9:1) as
eluant, to give the title compound (70 mg) as a colourless oil.
[0657] MS [M+H].sup.+ (ES+) 378
[0658] .sup.1H NMR: (CDCl.sub.3) .delta. 1.36-1.40 (2H, m),
1.75-1.85 (4H m), 2.0 (2H, t), 2.1-2.2 (2H, m), 2.4-2.45 (1H m),
2.6-2.7 (3H, m), 2.75-2.79 (2H, m), 3.4 (2H, s), 7.1-7.54 (5H, d),
8.44 (2H, m).
EXAMPLE 386
2-[(1,1'-Biphenyl)-4-yloxy]-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]acetam-
ide
[0659] 143
[0660] MS [M+H].sup.+ (ES+) 469
[0661] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.7-1.8
(2H, m), 2.0-2.1 (2H, m), 2.5-2.6 (2H, m), 3.45 (2H, s), 3.65-3.7
(1H, m), 4.5 (2H, s), 7.25-7.3 (2H, m), 7.27-7.63 (9H, m), 8.0 (1H,
d).
EXAMPLE 387
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-4-phenyl-3-butenamide
[0662] 144
[0663] MS [M+H].sup.+ (ES+) 403
[0664] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.40 (2H, m),
1.85-1.95 (2H, d), 2.05-2.15 (2H, t), 2.75-2.79 (2H, d), 3.1 (2H,
d), 3.4 (2H, s), 3.85-3.95 (1H, m), 5.45 (1H, m), 6.3 (1H, m), 6.5
(1H, d), 7.07-7.43 (8H, m).
EXAMPLE 388
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-(3-methoxyphenyl)-2-propenamide
[0665] 145
[0666] MS [M+H].sup.+ (ES+) 419
[0667] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 2.0
(2H, m), 2.15-2.25 (2H, m), 2.75-2.85 (2H, m), 3.4 (2H, s), 3.8
(3H, s), 3.94-4.05 (1H, m), 5.5 (1H, d), 6.35-6.4 (1H, d), 6.9-7.5
(7H, m), 7.6 (1H, d).
EXAMPLE 389
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-(4-iodophenoxy)propanamide
[0668] 146
[0669] MS [M+H].sup.+ (ES+) 533
[0670] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.9
(2H, d), 2.1-2.2 (2H, t), 2.6 (2H, m), 2.75-2.85 (2H, d), 3.4 (2H,
d), 3.8-3.9 (1H, m), 4.20 (2H, m), 6.65-6.7 (2H m), 7.1-7.2 (1H,
d), 7.35-7.45 (2H, m), 7.54-7.6 (2H, m).
EXAMPLE 390
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N'-(4-methoxyphenyl)succinamide
[0671] 147
[0672] MS [M+H].sup.+ (ES+) 464
[0673] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4 (2H, m), 1.6-1.65 (2H,
m), 2.05 (2H, m), 2.45 (2H, m), 2.65-2.75 (2H, m), 3.0 (2H, m) 3.45
(2H, s), 3.5 (1H, m), 3.7 (3H, s), 5.9 (1H, m), 6.85 (2H, d),
7.3-7.6 (4H, m), 7.7 (1H, d), 9.7 (1H, s).
EXAMPLE 391
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[(5-phenyl-2-pyrimidinyl)oxy]ac-
etamide
[0674] 148
[0675] MS [M+H].sup.+ (ES+) 471
[0676] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.9-2.0
(2H, m), 2.0-2.1 (2H, m), 2.75-2.85 (2H, m), 3.4 (2H, s), 3.9-4.0
(1H, m), 4.92 (2H, s), 6.53 (1H, d), 7.1-7.6 (7H, m), 7.7 (1H, s),
8.76 (2H, s).
EXAMPLE 392
N-[1-(4-iodobenzyl)-4-piperidinyl]-2-(5-phenyl-2-pyrimidinyl)thio]acetamid-
e
[0677] 149
[0678] MS [M+H].sup.+ (ES+) 545
[0679] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.8
(2H, m), 2.1-2.2 (2H, t), 2.65 (2H, m), 3.4 (2H, s), 3.9(3H, m),
6.8 (1H, d), 7.0 (2H, m), 7.5-7.7 (7H, m), 8.8 (2H, d).
EXAMPLE 393
N-[1-(3,4-dichlorobenzy)-4-piperidinyl]-2-[(2-pyrimidinyl)thio]acetamide
[0680] 150
[0681] MS [M+H].sup.+ (ES+) 412
[0682] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.8
(2H, m), 2.1 (2H, m), 2.65 (2H, m), 3.4 (2H, s), 3.8 (3H, m), 6.90
(1H m), 7.05-7.2 (4H, m), 8.58 (2H, d).
EXAMPLE 394
2-[(5-Bromo-2-pyrimidinyl)thio]-N-[1-(3,4-dichlorobenzy)-4-piperidinyl]ace-
tamide
[0683] 151
[0684] MS [M+H].sup.+ (ES+) 491
[0685] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.8
(2H, m), 2.15 (2H, m), 2.6 (2H, m), 3.4 (2H, s), 3.8 (3H, m), 6.6
(1H, d), 7.1 (1H m), 7.3-7.4 (2H, m) 8.58 (2H, d).
EXAMPLE 395
N-[1-(3,4-difluororobenzyl)-4-piperidinyl]-2-(4-pyridinylthio)acetamide
[0686] 152
[0687] MS [M+H].sup.+ (ES+) 378
[0688] .sup.1H NMR: (CDCl.sub.3) .delta. 1.36-1.40 (2H, m), 1.8
(2H, m), 2.05 (2H m), 2.65 (2H, m), 3.4 (2H,m), 3.67 (2H, s), 3.8
(1H, m), 6.5 (1H, m), 6.9-7.24 (4H, m) 8.48 (2H, d).
EXAMPLE 396
N-[1-(3,4-dichlorobenzy)-4-piperidinyl]-3-(5-phenyl-1H-pyrrol-2-yl)propana-
mide
[0689] 153
[0690] MS [M+H].sup.+ (ES+) 454
[0691] .sup.1H NMR: (CDCl.sub.3) .delta. 1.36-1.40 (2H, m), 1.87
(2H, m), 2.05 (2H m), 2.5 (2H, m), 2.65 (2H, m), 2.96 (2H, m), 3.4
(2H, s), 3.8 (1H, m), 5.35 (1H, d), 5.95-6.0 (1H, m) 6.38 (1H, m),
7.1-7.5 (8H, m), 9.5 (1H m).
EXAMPLE 397
N-[1-(3,4-dichlorobenzy)-4-piperidinyl]-N'-(5-phenyl-2-pyrimidinyl)-1,2-et-
handiamine
[0692] 154
[0693] The title compound (20 mg) was prepared by heating at reflux
N.sup.1-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-1,2-ethanediamine
(100 mg) and 2-chloro-5-phenypyrimidine (100 mg) and Hunigs' base
(10 mg) in toluene for 8 hours. The mixture was purified by
chromatography on silica, with ethyl acetate methanol (9:1) as
eluant to give the title compound as a yellow oil.
[0694] MS [M+H].sup.+ (ES+) 456/8
[0695] .sup.1H NMR: (CDCl.sub.3) .delta. 1.51 (2H, m), 1.75 (2H,
m), 2.15 (2H, td), 2.9 (2H, m), 3.05 (1H, m), 3.15 (2H, m), 3.44
(2H, m), 3.8 (2H, m), 6.65 (1H, m), 7.0-7.4 (8H, m), 8.5 (2H,
m).
EXAMPLE 398
N-[5-bromo-2-pyrimidinyl]-N'-[1-(3,4-dichlorobenzy)-4-piperidinyl-1,2-etha-
ndiamine
[0696] 155
[0697] Prepared by the method of Example 397 amine (200 mg),
2-chloro-5-bromopyrimidine (130 mg), Hunigs' base (200 mg) to give
the title compound (20 mg).
[0698] MS [M+H].sup.+ (ES+) 458/60
[0699] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4 (2H, m), 1.75 (2H, m),
2.05 (2H, td), 2.85 (2H, m), 3.0 (1H, m), 3.15 (2H, m), 3.44 (2H,
m), 3.75 (2H, m), 6.8 (1H, m), 7.0-7.4 (3H, m), 8.25 (2H, m).
EXAMPLE 399
2-[(2-Chloro-4-pyrimidinyl)amino]-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-
acetamide
[0700] 156
[0701] MS [M+H].sup.+ (ES+) 430/32
[0702] .sup.1H NMR: (CDCl.sub.3) .delta. 1.40-1.45 (2H, m), 1.97
(2H, m), 2.15 (2H m), 2.75 (2H, d), 3.4 (2H, s), 3.8 (1H, m), 4.05
(2H, d), 5.75 (1H, d), 5.84 (1H m), 6.38 (1H, d), 7.1-7.15 (1H d),
7.36-7.42(2H m), 8.0 (1H d).
EXAMPLE 401
2-[(5-Bromo-2-pyrimidinyl)oxy]-N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2--
acetamide
[0703] 157
[0704] MS [M+H].sup.+ (ES+) 475
[0705] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.87
(2H, m), 2.15 (2H, m), 2.75 (2H, m), 3.4 (2H, s), 3.9 (1H, m) 4.8
(2H, s), 6.38 (1H, d), 7.1-7.15 (1H m), 7.4 (2H, m), 8.6 (2H,
s).
EXAMPLE 402
N-[1-(3,4-dichlorobenzy)-4-piperidinyl]-2-(1,3-dioxo-1,3-dihydro-2H-isoind-
ol-2-yl)acetamide
[0706] 158
[0707] MS [M+H].sup.+ (ES+) 446
[0708] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46-1.50 (2H, m), 1.87
(2H, m), 2.15 (2H m), 2.75 (2H, m), 3.4 (2H, s), 3.8 (1H, m), 4.3
(2H s), 5.65 (1H, m), 7.1-7.36 (3H, m) 7.38-7.78 (2H, m), 7.87-7.95
(2H, m).
EXAMPLE 403
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N-[2-(2-pyridinylthio)ethylamine,
dihydrochloride
[0709] 159
[0710] .sup.1H NMR: (CDCl.sub.3) (of the free base): .delta. 1.4
(2H, m) 1.85 (2H, m), 2.05 (2H, m) 2.55 (2H, td), 2.8 (2H, m), 3.0
(1H, m), 3.3 (2H, m), 3.42 (2H, s), 6.9-7.5 (4H, m), 8.5 (2H,
m).
EXAMPLE 404
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-(phenylthio)propanamide
[0711] 160
[0712] MS [M+H].sup.+ (ES+) 423
[0713] .sup.1H NMR: (CDCl.sub.3) .delta. 136-1.40 (2H, m), 1.87
(2H, m), 2.15 (2H m), 2.45 (2H, m), 2.76 (2H, m), 3.2 (2H, m), 3.4
(2H, s), 3.8 (1H, m), 5.4 (1H, d), 7.1-7.5 (8H m).
EXAMPLE 405
N'-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-[trifluoromethoxy)phenoxy]acet-
ohydrazide
[0714] 161
[0715] The title compound was prepared from
3,4-dichlorobenzyl-4-piperidon- e (J. Med. Chem, 1999, 42, 3629;
100 mg), 2-[4-(trifluoromethoxy)phenoxy]a- cetohydrazide (100 mg),
sodium triacetoxyborohydride (100 mg), and 0.02 ml acetic acid,
stirred together for 2 hours in dichloromethane by the method of
Example 369 step ii.
[0716] MS [M+H].sup.+ (ES+) 492
[0717] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4-1.6 (3H, m) 1.7 (2H,
m), 2.0 (2H, m) 2.7-2.9 (2H, m), 3.4 (2H, m), 4.4 (3H, m), 5.3 (1H,
s) 6.9 (2H, m), 7.2-7.5 (4H, m), 7.8 (1H, d).
EXAMPLE 406
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N-[3-[3-(2-pyridinyl)-1,2,4-oxadi-
azo-5-yl]propyl]amine
[0718] 162
[0719] The title compound (29 mg) was prepared from
3,4-dichlorobenzylpiperidine-4-amine (100 mg free base),
2-[5-(3-bromopropyl)-1,2,4-oxadiazol-3-yl]pyridine (100 mg),
potassium carbonate (100 mg) in dimethyl formamide (1 ml) were
heated together in the microwave for 30 secs, water was added and
the product extracted into dichloromethane and chromatographed on
silica with ethyl acetate/methanol (9:1) as eluant.
[0720] MS [M+H].sup.+ (ES+) 446
[0721] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4 (2H, m) 1.7-1.9 (4H,
m), 2.0-2.1 (4H, m) 2.46 (1H, m), 2.75 (2H, m), 3.1 (2H, t), 3.4
(2H, s), 7.15-7.45 (4H, m), 7.85(1H, t) 8.1 (1H, d) 8.8 (1H,
d).
EXAMPLE 407
N-[2-[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino]ethyl]-3-(methylsulphonyl-
)benzamide
[0722] 163
[0723] Prepared from
N-(2-aminoethyl)-N-[1-(3,4-dichlorobenzyl)-4-piperidi-
nyl]-2,2,2-trifluoroacetamide (100 mg), 3-methylsulphonylbenzoic
acid (50 mg) and carbonyldiimidazole (40 mg). The product obtained
was stirred together with sodium hydroxide (40 mg) in 50:50
methanol/water for 12 hrs, extracted into dichloromethane and
purified by chromatography on silica with ethyl acetate/methanol
(9:1) as eluant, to give the title compound (25 mg).
[0724] MS [M+H].sup.+ (ES+) 485
[0725] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4 (2H, m) 1.9 (2H, m),
2.0-2.1 (1H, m) 2.6 (1H, m), 2.8 (2H, m), 2.95 (2H, m) 3.1 (3H, m)
3.4 (2H, s), 3.6 (2H, m), 7.15 (2H, m), 7.4 (2H, m), 7.65(1H, t)
8.1 (2H d) 8.4 (1H, d).
EXAMPLE 408
3-[5-(4-chlorophenyl-4H-1,2,4-triazol-3-yl]-N-[1-(3,4-dichlorobenzyl)-4-pi-
peridinyl])propanamide
[0726] 164
[0727] MS [M+H].sup.+ (ES+) 493
[0728] .sup.1H NMR: (CDCl.sub.3) .delta. 1.6 (2H, m), 1.87 (2H, m),
2.25 (2H m), 2.65 (2H, m), 2.86 (96(2H, m), 3.14 (2H, m), 3.5 (2H,
s), 3.85 (1H, m), 6.0 (1H, m) 7.23 (1H, m), 7.4 (3H m), 7.45 (1H
m), 8.0 (2H m).
EXAMPLE 409
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-3-(2-pyridinyl)propanamide
[0729] 165
[0730] MS [M+H].sup.+ (ES+) 394
[0731] .sup.1H NMR: (CDCl.sub.3) .delta. 1.46 (2H, m), 1.8 (2H, m),
2.15 (2H m), 2.75 (4H, m), 3.3 (2H m), 3.45 (2H, s), 3.8 (1H, m),
6.05 (1H, m), 7.1 (2H, m) 7.38 (1H, m), 7.45 (1H m), 8.65 (2H
m)
EXAMPLE 410
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-4-(4-(methylsulphonyl)phenyl-4-ox-
obutanamide
[0732] 166
[0733] MS [M+H].sup.+ (ES+) 497
[0734] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4-1.5 (2H, m), 1.9 (2H,
m), 2.15 (2H m), 2.65 (2H, m), 2.78 (2H m), 3.1 (3H s), 3.35 (2H
m), 3.4 (2H, s), 3.8 (1H, m), 5.55 (1H, m), 7.16 (1H, m) 7.38 (2H,
m), 8.05 (2H m), 8.2 (2H m).
EXAMPLE 411
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N'-[4-(methylsulphonyl)benzylamin-
e
[0735] 167
[0736] MS [M+H].sup.+ (ES+) 427
[0737] .sup.1H NMR: (CDCl.sub.3) .delta. 1.4 (2H, m), 1.8-1.9 (2H,
m), 2.0 (2H, m), 2.5 (2H, td), 2.8 (2H, m), 3.0 (2H, s), 3.4 (2H,
s), 3.94 (2H, s), 7.15 (1H, m), 7.4 (2H, m), 7.55 (2H, d) 7.9 (2H,
d).
EXAMPLE 412
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-N'-[(2-pyridinyl)succinamide
[0738] 168
[0739] MS [M+H].sup.+ (ES+) 435
[0740] .sup.1H NMR: (CDCl.sub.3) .delta. 1.6 (2H, m), 1.87 (2H, m),
2.05 (2H m), 2.65 (2H, m), 2.76 (4H m), 3.4 (2H, s), 3.8 (1H m),
5.65 (1H, m), 7.0 (1H, m), 7.1 (1H, m), 7.38 (2H, d), 7.7 (1H m),
8.2 (1H m), 8.27 (1H m), 8.65 (1H m).
EXAMPLE 413
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-(4-phenyl-1,3-thiazol-2-yl))ace-
tamide
[0741] 169
[0742] MS [M+H].sup.+ (ES+) 461
[0743] .sup.1H NMR: (CDCl.sub.3) .delta. 1.50 (2H, m), 1.87 (2H,
m), 2.15 (2H m), 2.65 (2H, m), 3.4 (2H, s), 3.85 (1H m), 4.0 (2H,
s), 7.15 (1H, d) 73-7.5 (6H, m), 7.9 (2H d).
EXAMPLE 414
N-[1-(3,4-dichlorobenzyl)-4-piperidinyl]-2-(2-phenyl-1,3-thiazol-4-yl))ace-
tamide
[0744] 170
[0745] MS [M+H].sup.+ (ES+) 461
[0746] .sup.1H NMR: (CDCl.sub.3): .delta. 1.45 (2H, m), 1.90 (2H,
m), 2.15 (2H m), 2.65 (2H, m), 3.25 (2H, s), 3.7 (2H, s), 3.85 (1H,
m), 7.15 (2H, m) 7.4 (2H, d), 7.5 (3H m), 8.0 (2H m).
EXAMPLE 415
N-[1-(3,4-difluorobenzyl)-4-piperidinyl]-3-(3-2-pyridinyl-1,2,4-oxadiazol--
5-yl]propanamide
[0747] 171
[0748] MS [M+H].sup.+ (ES+) 428
[0749] .sup.1H NMR: (CDCl.sub.3) .delta. 1.36-1.45 (2H, m), 2.0
(2H, m), 2.1-2.2 (2H, t), 2.7-2.85 (4H, m), 3.34 (2H, d), 3.4(2H,
d), 3.8 (1H, m), 5.6 (1H, d), 7.0-7.2 (3H m), 7.4 (1H m), 7.8 (1H,
m) 8.1 (1H, d), 8.8 (1H, d)
EXAMPLE 416
N-trifluoroacetyl-N-[2-[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino]ethyl]--
3-methoxybenzamide
[0750] 172
a) tert-butyl
2-{[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino}ethylcarbamat- e
[0751] The sub-title compound (800 mg) was prepared from
3,4-dichlorobenzyl-4-piperidone (1.3 g) tert-butyl
2-aminoethylcarbamate (0.8 g), sodium triacetoxyborohydride (10
mg), and 0.02 ml acetic acid, stirred together for 2 hrs in
dichloromethane. The sub-titled compound was isolated by standard
procedures.
[0752] MS [M+H].sup.+ (ES+) 402
b)
N-(2-aminoethyl)-N-[1-(3,4-dichlorobenzyl-4-piperidinyl]-2,2,2-trifluor-
oacetamide
[0753] A mixture of the above amine (800 mg), and triethylamine
(0.5 ml) in dichloromethane (50 ml), treated with trifluoroacetic
anhydride (420 mg) over 30 mins, evaporated to dryness and
dichloromethane (20 ml) and trifluoroacetic acid (2 ml) added,
stirred for 3 hrs, then neutralised with aqueous sodium
bicarbonate, the organic phase separated, dried and evaporated to
give the title compound (250 mg) as a yellow oil.
[0754] MS [M+H].sup.+ (ES+) 496/8
c)
N-trifluoroacetyl-N-[2-[1-(3,4-dichlorobenzyl)-4-piperidinyl]amino]ethy-
l]-3-methoxybenzamide
[0755] The title compound (30 mg) was prepared from the product
above (40 mg) 3-methoxybenzoyl chloride (20 mg) and triethylamine
(50 mg) using one of the methods described above.
[0756] MS [M+H].sup.+ (ES+) 580
[0757] .sup.1H NMR: (CDCl.sub.3) .delta. 0.9 (6H, m) 1.2-1.4 (6H,
m), 1.6-1.85 (4H, m) 2.8 (1H, m), 3.3 (4H, m), 3.6-3.8 (5H, m), 3.8
(2H, s), 7.0 (1H, m), 7.1 (1H, m), 7.35-7.45 (3H, m), 8.25(1H,
t).
[0758] Further compounds of formula (I), all according to formula
(Ic), are shown in the table below.
2 (Ic) 173 Example R.sup.1 (Q).sub.m (CR.sup.2R.sup.3).sub.n
T.sup.1 R* Z R.sup.6 380 4-Cl--C.sub.6H.sub.4 O CH.sub.2 C(O) H
CH.sub.2C(O)NH 2-Cl-5- CH.sub.3--C.sub.6H.sub.3 381
4-Cl--C.sub.6H.sub.4 O CH.sub.2 C(O) H (CH.sub.2).sub.3
C.sub.6H.sub.5 382 3-(pyridin-2-yl)- O CH.sub.2 C(O) H allyl
C.sub.6H.sub.5 1,2,4-oxadiazol-5-yl 383 2-(cyclopropyl-NH)- m = 0 n
= 0 -- CH.sub.3 CH.sub.2 3,4-Cl.sub.2-- pyrimidin-4-yl
C.sub.6H.sub.3 384 2-(pyridin-3-yl)- m = 0 n = 0 -- CH.sub.3
CH.sub.2 3,4-Cl.sub.2-- pyrimidin-4-yl C.sub.6H.sub.3 400
pyrimidin-2-yl S CH.sub.2 C(O) H C(O) 3,4-Cl.sub.2--
C.sub.6H.sub.3
[0759] Pharmacological Analysis
[0760] Calcium Flux [Ca.sup.2+].sub.i assay
[0761] a) Human Eosinophils
[0762] Human eosinophils were isolated from EDTA anticoagulated
peripheral blood as previously described (Hansel et al., J.
Immunol. Methods, 1991, 145, 105-110). The cells were resuspended
(5.times.10.sup.6 ml.sup.-1) and loaded with 5 .mu.M
FLUO-3/AM+Pluronic F127 2.2 .mu.l/ml (Molecular Probes) in low
potassium solution (LKS; NaCl 118 mM, MgSO.sub.4 0.8 mM, glucose
5.5 mM, Na.sub.2CO.sub.3 8.5 mM, KCl 5 mM, HEPES 20 mM, CaCl.sub.2
1.8 mM, BSA 0.1%, pH 7.4) for one hour at room temperature. After
loading, cells were centrifuged at 200 g for 5 min and resuspended
in LKS at 2.5.times.10.sup.6 Ml.sup.-1. The cells were then
transferred to 96 well FLIPr plates (Poly-D-Lysine plates from
Becton Dickinson pre-incubated with 5 .mu.M fibronectin for two
hours) at 100 ml/well. The plate was centrifuged at 200 g for 5 min
and the cells were washed twice with LKS (200 .mu.l; room
temperature).
[0763] A compound of the Examples was pre-dissolved in
dimethylsulphoxide and added to a final concentration of 0.1% (v/v)
dimethylsulphoxide. Assays were initiated by the addition of an
A.sub.50 concentration of eotaxin and the transient increase in
fluo-3 fluorescence (I.sub.Ex=490 nm and I.sub.Em=520 nm) monitored
using a FLIPR (Fluorometric Imaging Plate Reader, Molecular
Devices, Sunnyvale, U.S.A).
[0764] b) Human Monocytes
[0765] Human monocytes were isolated from EDTA anticoagulated
peripheral blood as previously described (Cunoosamy & Holbrook
J. Leukocyte Biology, 1998, S2, 13). Cells were resuspended
(5.times.10.sup.6 ml.sup.-1) in LKS and loaded with 5 .mu.M
FLUO3/AM+Pluronic F127 2.2 .mu.l/ml (Molecular Probes) for one hour
at room temperature. After loading, cells were centrifuged at 200 g
for 5 min and resuspended in LKS at 0.5.times.10.sup.6 ml.sup.-1.
The cells were then transferred to 96 well FLIPr plates (Costar).
To each well 100 .mu.l of cells were added at a concentration of
0.5.times.10.sup.6 ml.sup.-1. The plates were centrifuged (200 g, 5
mins; room temperature) to allow the cells to adhere. After
centrifugation the cells were washed twice with LKS (200 .mu.l;
room temperature).
[0766] A compound of the Examples was pre-dissolved in
dimethylsulphoxide and added to a final concentration of 0.1% (v/v)
dimethylsulphoxide. Assays were initiated by the addition of an
A.sub.50 concentration of MIP-1.alpha. and the transient increase
in fluo-3 fluorescence (I.sub.Ex=490 nm and I.sub.Em=520 nm)
monitored using a FLIPR (Fluorometric Imaging Plate Reader,
Molecular Devices, Sunnyvale, U.S.A).
[0767] The compounds of the Examples were found to be antagonists
of the eotaxin mediated [Ca.sup.2+].sub.i in human eosinophils
and/or antagonists of the MIP-1.alpha. mediated [Ca.sup.2+].sub.i
in human monocytes.
[0768] Human Eosinophil Chemotaxis
[0769] Human eosinophils were isolated from EDTA anticoagulated
peripheral blood as previously described (Hansel et al., J.
Immunol. Methods, 1991, 145 105-110). The cells were resuspended at
10.times.10.sup.6 ml.sup.-1 in RPMI containing 200 IU/ml
penicillin, 200 .mu.g/ml streptomycin sulphate and supplemented
with 10% HIFCS, at room temperature.
[0770] Eosinophils (700 .mu.l) were pre-incubated for 15 mins at
37.degree. C. with 7 .mu.l of either vehicle or compound
(100.times. required final concentration in 10%
dimethylsulphoxide). The chemotaxis plate (ChemoTx, 3 .mu.m pore,
Neuroprobe) was loaded by adding 28 .mu.l of a concentration of
eotaxin (0.1 to 100 nM) containing a concentration of a compound
according to the Examples or solvent to the lower wells of the
chemotaxis plate. The filter was then placed over the wells and 25
.mu.l of eosinophil suspension were added to the top of the filter.
The plate was incubated for 1 hr at 37.degree. C. in a humidified
incubator with a 95% air/5% CO.sub.2 atmosphere to allow
chemotaxis.
[0771] The medium, containing cells that had not migrated, was
carefully aspirated from above the filter and discarded. The filter
was washed once with phosphate buffered saline (PBS) containing 5
mM EDTA to remove any adherent cells. Cells that had migrated
through the filter were pelleted by centrifugation (300.times.g for
5 mins at room temperature) and the filter removed and the
supernatant transferred to each well of a 96-well plate (Costar).
The pelleted cells were lysed by the addition of 28 .mu.l of PBS
containing 0.5% Triton.times.100 followed by two cycles of
freeze/thawing. The cell lysate was then added to the supernatant.
The number of eosinophils migrating was quantified according to the
method of Strath et al., J. Immunol. Methods, 1985, 83, 209 by
measuring eosinophil peroxidase activity in the supernatant.
[0772] Certain compounds of the Examples were found to be
antagonists of the eotaxin mediated human eosinophil
chemotaxis.
* * * * *