Reagents and methods for identifying and modulating expression of genes regulated by retinoids

Abstract

This invention identifies growth-inhibitory genes induced by retinoids. The invention provides reagents and methods for identifying compounds other than retinoids that induce expression of these cellular genes. The invention also provides reagents that are recombinant mammalian cells containing recombinant expression constructs that express a reporter gene under the transcriptional control of a promoter for a gene that is regulated by retinoids, and methods for using such cells to identify compounds other than retinoids that modulate expression of these cellular genes.

Description

[0001] This application claims priority to U.S. Provisional Application Ser. No. 60/207,535, filed May 26, 2000.

BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] This invention is related to changes in cellular gene expression and compounds that produce changes in cellular gene expression. In particular, the invention is related to the identification of genes the expression of which is modulated by a class of compounds known as retinoids, being chemically related to retinoic acid and Vitamin A, More specifically, the invention provides methods for identifying compounds other than retinoids that modulate expression of these cellular genes. The invention also provides reagents that are recombinant mammalian cells containing recombinant expression constructs that express a reporter gene under the transcriptional control of a promoter for a gene that is regulated by retinoids, and methods for using such cells for identifying compounds other than retinoids that modulate expression of these cellular genes.

[0005] 2. Summary of the Related Art

[0006] Retinoids are naturally-occurring or synthetic derivatives of vitamin A. They comprise a class of clinically important differentiation agents that regulate cell growth and differentiation at the level of transcription, by binding to nuclear receptors that act as ligand-dependent transcription factors. These compounds induce cellular differentiation or terminal proliferation arrest in a number of tumor cell types in vivo and in vitro that express retinoid receptors (Warrell, 1997, in CANCER. PRINCIPLES & PRACTICE OF ONCOLOGY (DeVita et al., eds.), pp.483-490 (Lippincott-Raven, Philadelphia), making them useful for treating some cancers, such as acute promyelocytic leukemia and cancer chemoprevention.

[0007] The target of retinoid action is the cell nucleus, where retinoids bind to two types of receptors, termed RARs (retinoic acid receptors) and RXRs (retinoid X receptors) (Mangelsdorf et al., 1994, "The retinoid receptors," in: The Retinoids: biology, chemistry, and medicine, Sporn et al., eds., New York: Raven Press, pp. 319-351.) Retinoid-bound receptor molecules form homo- (RXR-RXR) and heterodimers (RAR-RXR) that act as transcription factors. These dimers bind to specific cis-regulatory sequences in the promoters of retinoid-responsive target genes, termed RARE (Retinoic Acid Response Elements), regulating their transcription. The resulting changes in gene expression are caused either directly by retinoid receptor regulation of target gene expression, or indirectly through the action of retinoid-activated signal transduction pathways, for example, pathways activated by the transcription factor AP-1. These gene expression changes are ultimately responsible for the growth-inhibitory effect of retinoids (Warrell, Id.).

[0008] Although retinoids have had some clinical success in cancer treatment, their use has been limited by at least two factors: development of resistance (Miller et al., 1998, Cancer 83: 1471-1482) or toxicity. Development of resistance is due in part to alterations of retinoic acid receptors and retinoid receptor-mediated pathways (Miller et al., ibid.). Toxicity is generally attributed to the broad physiologic consequences of retinoids, resulting from pleiotropic changes in gene expression produced by treatment with retinoids.

[0009] Several growth-inhibitory genes have been previously found to be inducible by retinoids in epithelial cells. None of these genes, however, was shown to be solely responsible for the growth-inhibitory effect of retinoids.

[0010] Adamo et al., 1992, Endocrinology 131: 1858-1866 disclosed induction of insulin-like growth factor binding protein 3 (IGFBP-3) in breast carcinoma cell lines.

[0011] Swisshelm et al., 1995, Proc. Natl. Acad. Sci. USA 92: 4472-4476 identified another insulin-like growth factor binding protein, IGFBP-7 (also known as insulin-like growth factor binding protein related protein 1, or IGFBP-rP1, and mac25) as a protein induced by treatment of cells with fenretinide (4-hydroxyphenylretinamide, 4-HPR). This protein was also shown to be down-regulated in mammary carcinoma cell lines.

[0012] Kato et al., 1996, Oncogene 12: 1361-1364 showed that introduction of mac25 cDNA into an osteosarcoma cell line inhibited growth.

[0013] Gucev et al., 1996, Cancer Res. 56: 1545-1550 identified IGFBP-3 as a protein induced in breast carcinoma cells both by all-trans retinoic acid (RA) and by transforming growth factor .beta. (TGF-.beta.). RA-mediated growth inhibition in these cells was alleviated by introducing an antisense oligonucleotide into the cells that inhibited IGFBP-3 expression, or by introducing exogenous IGFBP-3 into the cells.

[0014] DiSepio et al., 1998, Proc. Natl. Acad. Sci. USA 95: 14811-14815 showed that tazarotene-induced gene 3 (TIG-3, also known as retinoid-inducible gene 1, RIG-1), a putative tumor suppressor gene, is induced in primary human keratinocytes. TIG-3 shows decreased expression in cancer cells, inhibits the growth of cancer cells when expressed, and shares sequence homology with a known tumor suppressor gene, H-rev 107.

[0015] Huang et al., 2000, Molec. Cell. Endocrinol. 159: 15-24 showed that TIG-3 was induced by retinoids in a gastric carcinoma cell line in vitro.

[0016] Liu et al., 2000, J. Cancer Res. Clin. Oncol. 126: 85-90 reported that RA-induced expression of a metastasis suppressor gene, nm23-H1 in a hepatocarcinoma cell line.

[0017] The teachings of the prior art suggest that one mechanism of retinoid-mediated growth inhibition is the activation (or re-activation) of tumor suppressor genes and other growth-inhibitory genes that have been repressed or whose expression has been down-regulated in tumor cells. However, the reports in the art fail to indicate the identity or number of growth-inhibitory genes that are activated under the conditions of retinoid-induced growth arrest. Such reports also fail to indicate if retinoid-induced genes are induced by retinoids directly, through RARE sites in their promoters, or indirectly. In the latter case, it should be possible to activate such growth-inhibitory genes even in the cells that are not responsive to retinoids.

[0018] There remains a need in this art to identify genes whose expression is modulated by retinoids, and especially growth-inhibitory genes that are induced by retinoids indirectly. There is also a need in this art to develop methods for assessing the effects of compounds on expression of retinoid-modulated cellular genes, particularly growth-inhibitory genes. There is an additional need to develop alternative compounds that mimic the effects of retinoids on cellular gene expression, to which resistance is not so easily developed and that lack the toxicity and other systemic side-effects of retinoids in current clinical use.

SUMMARY OF THE INVENTION

[0019] This invention provides genes whose expression is modulated by retinoids and reagents and methods for identifying compounds that mimic the effects of retinoids without producing resistance or toxicity to said compounds.

[0020] In a first aspect, the invention provides a recombinant expression construct encoding a reporter gene operably linked to a promoter from a gene the expression of which is induced by a retinoid and which does not contain a RARE site. In preferred embodiments, the reporter gene encodes firefly luciferase, chloramphenicol acetyltransferase, beta-galactosidase, green fluorescent protein, or alkaline phosphatase. Preferred retinoids include all-trans retinoic acid, fenretinide, 9-cis retinoic acid, 13-cis retinoic acid, etretinate and retinol (Vitamin A). Most preferred promoters comprising the recombinant expression constructs of the invention are promoters from a cellular gene that is known to be induced by a retinoid and to have a growth-inhibitory activity. In preferred embodiments, the cellular gene promoter is from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), proteasome activator PA28 subunit .alpha.(PA28.alpha.SEQ ID NO.:5), Mac-2 binding protein (Mac-2 BP; SEQ ID NO.:6), Protein C inhibitor (PCI; SEQ ID NO.:7), T cell receptor gamma (SEQ ID NO.:8), retinal oxidase (SEQ ID NO.:9), Bene (SEQ ID NO.: 10), HIF-2alpha/EPAS-1 (SEQ ID NO.: 11), or selectin L (SEQ ID NO.: 12). In particularly preferred embodiments, the promoter is from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), or proteasome activator PA28 subunit .alpha.(PA28.alpha.; SEQ ID NO. 5).

[0021] In a second embodiment, the invention provides a mammalian cell containing a recombinant expression construct of the invention encoding a reporter gene under the transcriptional control of a promoter from a retinoid-inducible cellular gene. In preferred embodiments, the reporter gene encodes firefly luciferase, chloramphenicol acetyltransferase, beta-galactosidase, green fluorescent protein, or alkaline phosphatase. Preferred retinoids include all-trans retinoic acid, fenretinide, 9-cis retinoic acid, 13-cis retinoic acid, etretinate and retinol. Most preferred promoters comprising the recombinant expression constructs of the invention are promoters from a cellular gene that is known to be induced by a retinoid and to have a growth-inhibitory activity or from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO.:5), Mac-2 binding protein (Mac-2 BP; SEQ ID NO.:6), Protein C inhibitor (PCI; SEQ ID NO.:7), T cell receptor gamma (SEQ ID NO.:8), retinal oxidase (SEQ ID NO.:9), Bene (SEQ ID NO.: 10), HIF-2alpha/EPAS-1 (SEQ ID NO.:11), or selectin L (SEQ ID NO.:12). In particularly preferred embodiments, the promoter is from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), or proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO. 5).

[0022] In a third embodiment, the invention provides a method for identifying a compound that induces expression of a retinoid-inducible gene in a mammalian cell. In this method, recombinant mammalian cells according to the invention containing a recombinant expression construct of the invention encoding a reporter gene under the transcriptional control of a promoter from a retinoid-inducible cellular gene are cultured under conditions that induce expression of at least one retinoid-induced gene in mammalian cells in the presence and absence of a compound. Reporter gene expression is compared in the cell in the presence of the compound with reporter gene expression in said cell in the absence of the compound. Compounds that induce retinoid-induced gene expression are identified if reporter gene expression is higher in the presence of the compound than in the absence of the compound. In preferred embodiments, the reporter gene encodes firefly luciferase, chloramphenicol acetyltransferase, beta-galactosidase, green fluorescent protein, or alkaline phosphatase. Preferred retinoids include all-trans retinoic acid, fenretinide, 9-cis retinoic acid, 13 -cis retinoic acid, etretinate and retinol. Most preferred promoters comprising the recombinant expression constructs of the invention are promoters of a cellular gene that is known to be induced by a retinoid and to have a growth-inhibitory activity or from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO.:5), Mac-2 binding protein (Mac-2 BP; SEQ ID NO.:6), Protein C inhibitor (PCI; SEQ ID NO.:7), T cell receptor gamma (SEQ ID NO.:8), retinal oxidase (SEQ ID NO.:9), Bene (SEQ ID NO.:10), HIF-2alpha/EPAS-1 (SEQ ID NO.:11), or selectin L (SEQ ID NO.:12). In particularly preferred embodiments, the promoter is from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), or proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO. 5). In preferred embodiments, expression of the reporter gene is detected using an immunological reagent, by hybridization to a complementary, detectably-labeled nucleic acid, or by detecting an activity of the reporter gene product.

[0023] In a fourth embodiment, the invention provides a method for identifying a compound that induces expression of a retinoid-inducible gene in a mammalian cell. In this aspect of the invention, mammalian cells are cultured in the presence and absence of the compound. The cells are then assayed for expression of at least one cellular gene whose expression is induced by a retinoid. Compounds that induce expression of a retinoid-inducible gene in a mammalian cell are identified if expression of the cellular genes of subpart (b) is higher in the presence of the compound than in the absence of the compound. Preferred retinoids include all-trans retinoic acid, fenretinide, 9-cis retinoic acid, 13-cis retinoic acid, etretinate and retinol. The gene is a cellular gene that is known to be induced by a retinoid and to have a growth-inhibitory activity or human insulin-like growth factor binding protein-3 (IGFBP-3; NCBI Accession No. M35878.1), secreted cell adhesion protein .beta.IG-H3 (Accession No. AC004503.1), epithelial protein lost in neoplasm (EPLIN; Accession No. AH009382. 1), ubiquitin-like protein FAT10 (Accession No. AL031983), Mac-2 binding protein (Mac-2 BP; Accession No. U91729), Protein C inhibitor (PCI; Accession No. AL049839.3), T cell receptor gamma (Accession No. AC006033.2), retinal oxidase (Accession No. AF010260), Bene (Accession No. AP001234.3), HIF-2alpha/EPAS-1 (Accession No. NT.sub.--005065.3), selectin L (Accession No. AL021940.1), or proteasome activator PA28 subunit .alpha.(PA28.alpha., Accession No. AL136295.2). In particularly preferred embodiments, the gene is human IGFBP-3, .beta.IG-H3, EPLIN, FAT10 or PA28.alpha.. In preferred embodiments, expression of the cellular gene is detected using an immunological reagent, by hybridization to a complementary, detectably-labeled nucleic acid, or by detecting an activity of the gene product.

[0024] The invention also provides methods for treating an animal having cancer to prevent or ameliorate the disease. In this aspect, a therapeutically-effective dose of a compound identified by the invention that induces expression of a retinoid-induced gene is administered to an animal, most preferably a human, in need thereof.

[0025] Specific preferred embodiments of the present invention will become evident from the following more detailed description of certain preferred embodiments and the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

[0026] FIGS. 1A and 1B are graphs showing the effects of retinoic acid (RA) on colony formation by MCF-7 cells. In each assay, 1.5.times.10.sup.5 cells were plated per P100, in the absence or in the presence of RA. For clonogenic assays, cells were trypsinized after treatment, and 2,500 cells were plated per P100 and grown in drug-free media. Colonies comprising at least 60-80 cells were scored 12-14 days after plating; these results were normalized by the number of colonies formed by untreated cells. Each point represents the mean and standard deviation for triplicate assays. FIG. 1A shows the effect of treatment with 100 nM RA for the indicated number of days. FIG. 1B shows the effect of 40 hr treatment with the indicated doses of RA.

[0027] FIGS. 2A through 2C show the results of RT-PCR analysis of changes in the expression of retinoid-inducible genes as described in Example 1. The identity of each gene is followed by the NCBI Accession Number (in parentheses). FIG. 2A shows a time course of changes in gene expression on the indicated days after the addition of 100 nM RA. The designations "R5" and "R8" correspond to days 5 and 8 after release from 5-day RA treatment, respectively. FIG. 2B shows the effects of 40 hr treatment with the indicated doses of RA. FIG. 2C shows a time course of changes in gene expression on the indicated days after the addition of 1 .mu.M fenretinide.

[0028] FIG. 3A is a photomicrograph and FIG. 3B is a photograph of an immunoblot showing induction of IGFBP-3, HIF2.alpha./EPAS- 1 and EPLIN proteins in RA-treated cells. FIG. 3A shows the results of immunocytochemical analysis of IGFBP-3, HIF2.alpha./EPAS-1 and EPLIN in untreated MCF-7 cells and in cells treated with 100 nM RA for 5 days. FIG. 3B shows immunoblotting analysis of EPLIN in untreated MCF-7 cells and in cells treated with 100 nM RA for the indicated number of days.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0029] This invention provides genes induced to express by retinoids. The invention also provides reagents and methods for identifying compounds that mimic the gene expression inducing properties of retinoids but lack toxicity and propensity for cells to develop resistance that is characteristic of retinoid treatment.

[0030] The present inventors have determined that retinoid treatment of retinoid-sensitive cells, such as human breast carcinoma MCF-7 cells, induces the expression of a group of genes that comprise a cohort of retinoid-inducible genes. Several of these genes are growth-inhibitory genes, i.e., genes whose expression inhibits the growth or tumorigenicity of tumor cells. These genes are important, inter alia, as targets to be induced in tumor cells and other cells that proliferate inappropriately or pathogenically to inhibit the growth thereof. It was known in the art that genes whose expression was regulatable by retinoids contained a specific class of sequences in their promoters, termed RARE (Mangelsdorfet al., 1994, in THE RETINOIDS: BIOLOGY, CHEMISTRY, AND MEDICINE, (Sporn et al., eds.), pp. 327-330 (Raven Press, New York). Surprisingly, however, all but one of the genes most strongly induced by retinoids in MCF-7 cells as determined by the inventors did not contain such RARE sequences in their promoters. This unexpected result indicated that retinoids activate these genes indirectly, by a mechanism that does not require RXR homodimer or RAR-RXR heterodimer binding to RARE sequences for these genes. This result also suggested that compounds other than retinoids should be capable of inducing expression of these (and perhaps other) growth-inhibitory genes in both retinoid-sensitive and retinoid-insensitive cells. Before the present invention, there was no reason to suspect that retinoid-insensitive cells could be induced to express retinoid-inducible growth inhibitory genes.

[0031] Disclosed herein is the inventors' discovery of 13 retinoid-inducible genes, including several genes having growth-inhibitory effects in mammalian cells. One of ordinary skill will appreciate that these results are not exhaustive, and other growth inhibitory genes may be induced by retinoids in mammalian cells. In view of the instant results, the skilled worker will also appreciate that some of these additional genes will be expected to lack RARE sequences in their promoters and thus be indirectly induced by retinoids. As disclosed herein, retinoid-inducible genes lacking RARE sequences in their promoters are useful targets for identifying compounds other than retinoids that mimic the physiologically-based growth inhibitory effect on cell proliferation. Identifying such compounds advantageously provides alternative agents for producing growth arrest in mammalian cells, particularly tumor cells and other cells that proliferate inappropriately or pathogenically. Such compounds are beneficial because they can mimic the growth-inhibitory effects of retinoids.

[0032] Another advantage of such compounds is that they can be expected to have a growth-inhibitory effect without producing systemic side effects found with other growth-inhibitory compounds known in the prior art. For example, many growth-inhibitory drugs and compounds known in the prior art disadvantageously induce p21 gene expression, which induces senescence, growth arrest and apoptosis by activating a plurality of genes, the expression of which is associated with the development of diseases, particularly age-related diseases such as Alzheimer's disease, atherosclerosis, renal disease, and arthritis (as disclosed in co-owned and co-pending U.S. Ser. No. 60/______, filed Feb. 1, 2001 (Attorney Docket No. 99,216-E) and U.S. Ser. No. 09/______, filed May 21, 2001 (Attorney Docket No. 99,216-F), incorporated by reference herein. Retinoic acid-induced growth inhibition in MCF-7 cells, in contrast, does not induce p21 (Zhu et al., 1997, Exp. Cell Res. 234: 293-299). The genes identified herein that are induced by retinoids are not known to be associated with any disease or disadvantageous or pathogenic effect when expressed in an animal. Thus, identification of such compounds that mimic the growth-inhibitory effects of retinoids by inducing expression of one or a plurality of the genes identified herein can be expected to have reduced or no such side-effects, making them better agents for anti-tumor and other therapies. Discovery of compounds that mimic the growth-inhibitory effects of retinoids without producing the toxic side effects of growth-inhibitory compounds known in the art is thus advantageously provided by the invention.

[0033] As provided herein, mammalian genes responsive to retinoids but not containing RARE sites in their promoters include human insulin-like growth factor binding protein-3 (IGFBP-3; NCBI Accession No. M35878.1), secreted cell adhesion protein .beta.IG-H3 (Accession No. AC004503.1), epithelial protein lost in neoplasm (EPLIN; Accession No. AH009382.1), ubiquitin-like protein FAT10 (Accession No. AL031983), Mac-2 binding protein (Mac-2 BP; Accession No. U91729), protein C inhibitor (PCI; Accession No. AL049839.3), T cell receptor gamma (Accession No. AC006033.2), retinal oxidase (Accession No. AF010260), Bene (Accession No. AP001234.3), HIF-2alpha/EPAS-1 (Accession No. NT.sub.--005065.3), selectin L (Accession No. AL021940.1), or proteasome activator PA28 subunit .alpha.(PA28.alpha., (4 Accession No. AL I 36295.2).

[0034] For the purposes of this invention, reference to "a cell" or "cells" is intended to be equivalent, and particularly encompasses in vitro cultures of mammalian cells grown and maintained as known in the art.

[0035] For the purposes of this invention, reference to "cellular genes" in the plural is intended to encompass a single gene as well as two or more genes. It will also be understood by those with skill in the art that effects of modulation of cellular gene expression, or reporter constructs under the transcriptional control of promoters derived from cellular genes, can be detected in a first gene and then the effect replicated by testing a second or any number of additional genes or reporter gene constructs. Alternatively, expression of two or more genes or reporter gene constructs can be assayed simultaneously within the scope of this invention.

[0036] As used herein, the term "RARE site" is intended to encompass two hexameric core motifs (as defined in Mangelsdorf et al., 1994, in THE RETINOIDS: BIOLOGY, CHEMISTRY, AND MEDICINE, (Spom et al., eds.), pp.327-330 (Raven Press, New York), separated by one, two or five nucleotides), wherein the hexameric motifs are arranged as direct, inverted or palindromic repeats.

[0037] The instant inventors have shown that treatment of MCF-7 human breast carcinoma cells with low doses of retinoids induces gradual growth arrest with minimal cytotoxicity and phenotypic features of cell senescence (Chang et al., 1999, Cancer Res. 59: 3761-3767). Relatively low doses of RA were found to induce irreversible growth arrest in MCF-7 cells, while producing only a minor reduction in cell numbers caused by cell death. This effect with "low dose" RA (between 10-100 nM) required 4-6 days of continuous exposure to RA to become apparent. Low-dose retinoid treatment was accompanied by the development of phenotypic changes in the treated cells characteristic for cellular senescence, including the development of an enlarged, flattened cellular morphology and expression of the senescence-associated marker, SA-.beta.-galactosidase (SA-.beta.-gal). Induction of SA-.beta.-gal was also observed in xenografts of MCF-10T neo mammary epithelial cells in vivo after treatment with fenretinide. These results suggested that retinoid treatment induces senescence in tumor cells in vivo and in vitro when administered in cytostatic doses.

[0038] Senescence can be induced in a mammalian cell in a number of ways known to those with skill in the art. For example, senescence is a natural consequence of normal cell growth, either in vivo or in vitro: there are a limited number of cell divisions, passages or generations that a normal cell can undergo before it becomes senescent. The precise number varies with cell type and species of origin (Hayflick & Moorhead, 1961, Exp. Cell Res. 25: 585-621). Senescence can also be induced in both normal and tumor cells by treatment with cytotoxic drugs such as most anticancer drugs or radiation. See, Changetal., 1999, Cancer Res. 59: 3761-3767. Senescence also can be rapidly induced in any mammalian cell by transducing into that cell a tumor suppressor gene (such as p53, p21, p16 or Rb) and expressing the gene therein. See, Sugrue et al., 1997, Proc. Natl. Acad. Sci. USA 94: 9648-9653; Uhrbom et al., 1997, Oncogene 15: 505-514; Xu et al., 1997, Oncogene 15: 2589-2596; Vogt et al., 1998, Cell Growth Differ. 9: 139-146. These and other means and methods for inducing senescence in mammalian cells will be appreciated and understood by those with skill in the art, and fall within the compass of this invention.

[0039] The reagents of the present invention include any mammalian cell, preferably a rodent or primate cell, more preferably a mouse cell and most preferably a human cell, that can induce cellular gene expression in response to a retinoid, wherein such gene is either the endogenous gene or an exogenous gene introduced by genetic engineering. Preferred cells include mammalian cells, preferably rodent or primate cells, and more preferably mouse or human cells.

[0040] Recombinant expression constructs can be introduced into appropriate mammalian cells as understood by those with skill in the art. Preferred embodiments of said constructs are produced in transmissible vectors, more preferably viral vectors and most preferably retrovirus vectors, adenovirus vectors, adeno-associated virus vectors, and vaccinia virus vectors, as known in the art. See, generally, MAMMALIAN CELL BIOTECHNOLOGY: A PRACTICAL APPROACH, (Butler, ed.), Oxford University Press: New York, 1991, pp. 57-84.

[0041] The invention also provides recombinant expression constructs wherein a reporter gene is under the transcriptional control of a promoter of a gene whose expression is induced by a retinoid. In preferred embodiments of this aspect of the invention, the retinoid is all-trans retinoic acid, fenretinide, 9-cis retinoic acid, 13-cis retinoic acid, etretinate or retinol. In preferred embodiments, the promoters are derived from genes whose expression is induced or otherwise increased by treatment of the cell with a retinoid, and preferably from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO.4), proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO.:5), Mac-2 binding protein (Mac-2 BP; SEQ ID NO.:6), Protein C inhibitor (PCI; SEQ ID NO.:7), T cell receptor gamma (SEQ ID NO.:8), retinal oxidase (SEQ ID NO.:9), Bene (SEQ ID NO.:10), HIF-2alpha/EPAS-1 (SEQ ID NO.:11), or selectin L (SEQ ID NO.:12). Most preferably, the promoter is derived from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO.4), or proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO.:5), Mac-2 binding protein (Mac-2 BP; SEQ ID NO.:6), Protein C inhibitor (PCI; SEQ ID NO.:7), T cell receptor gamma (SEQ ID NO.:8), retinal oxidase (SEQ ID NO.:9), Bene (SEQ ID NO.:10), HIF-2alpha/EPAS-1 (SEQ ID NO.:11), or selectin L (SEQ ID NO.:12). Most preferably, the promoter is derived from human insulin-like growth factor binding protein-3 (IGFBP-3; SEQ ID NO. 1), secreted cell adhesion protein .beta.IG-H3 (SEQ ID NO. 2), epithelial protein lost in neoplasm (EPLIN; SEQ ID NO. 3), ubiquitin-like protein FAT10 (SEQ ID NO. 4), or proteasome activator PA28 subunit .alpha.(PA28.alpha., SEQ ID NO. 5). These reporter genes are then used as sensitive and convenient indicators of the effects of retinoid-induced gene expression, and enable compounds that mimic the effects of retinoids in mammalian cells to be easily identified. Host cells for these constructs include any mammalian cell. Reporter genes useful in the practice of this aspect of the invention include but are not limited to firefly luciferase, chloramphenicol acetyltransferase, beta-galactosidase, green fluorescent protein, and alkaline phosphatase.

[0042] The following Examples are intended to further illustrate certain preferred embodiments of the invention and are not limiting in nature.

EXAMPLE 1

Analysis of Gene Expression Modulation by Treatment With Retinoic Acid

[0043] Cytological and gene expression analyses were performed to determine the effects of retinoic acid treatment on MCF-7 cells in culture.

[0044] Clonogenic assays were performed to analyze the differences in proliferative capacity in MCF-7 cells after treatment with 100 nM RA for varying times. Cells were exposed to 100 nM RA for 1-7 days and tested for their capacity to form colonies after treatment at each time point. As shown in FIG. 1A, plating efficiency, normalized to untreated control, decreased with time of cell incubation with 100 nM RA. The decrease was initially rapid (from 100% to 40% plating efficiency after 2 days incubation with RA) and decreased more slowly to a final plating efficiency of about 10% at day 7. FIG. 1B shows the plating efficiency of MCF-7 cells at varying concentrations of RA; these results show a dose-dependent reduction in plating efficiency at all tested doses (as low as 10 nM). Significant reduction in plating efficiency was observed at concentrations much lower than conventionally used (.gtoreq.1 .mu.M) to study the effects of retinoids on cell growth.

[0045] These results were consistent with a reduction in cell growth and proliferative capacity due to retinoid-modulated changes in cellular gene expression. To study gene expression changes, poly(A)+ RNA was isolated from untreated MCF-7 cells and from cells treated for 5 days with 100 nM RA. cDNA was prepared from these RNA populations and the cDNA hybridized with a cDNA microarray (Human UniGEM V cDNA microarray, Incyte Genomics, St. Louis, Mo.) that contains >7,000 cDNAs of different human genes and ESTs. cDNA probe synthesis, hybridization with the microarray and signal analyses were conducted by IncyteGenomics as a commercial service.

[0046] None of the genes in the microarray showed an increase in relative hybridization intensity over 2.5-fold or a decrease over 3-fold in RA-treated cells. Changes in RNA levels of a total of 47 genes that showed the biggest differences in microarray hybridization were tested by reverse transcription-PCR (RT-PCR) analysis. Of these, 27 genes showed 1.4-2.5 fold increase and 20 genes showed 1.7-3.0 fold decrease in `balanced differential expression` (a measure of relative hybridization intensity).

[0047] RT-PCR analysis was carried out essentially as described (Noonan et al., 1990, Proc. Natl. Acad. Sci. USA 87: 7160-7164), using .beta.-actin as an internal normalization standard. Sequences of RT-PCR primers and PCR conditions for thirteen genes most strongly induced by RA are as follows:

1TABLE I Oligonucleotide primers for performing PCR Gene Sense Primer (5' .fwdarw. 3') Antisense Primer (5' .fwdarw. 3') IGFBP-3 TTGCACAAAAGACTGCCAAG (SEQ ID NO:14) CATGAAGTCTGGGTGCTGTG (SEQ ID NO.:15) Mac-2 BP AATTCCACACTGTGCCCTTC (SEQ ID NO.:16) GTGGAGTCTGGAAGGACTGG (SEQ ID NO.:17) beta IG-H3 TGCGACTAGCCCCTGTCTAT (SEQ ID NO.:18) CATGCACAAGGCTCACATCT (SEQ ID NO.:19) PCI GCACCCAAGAGCAAGACTTC (SEQ ID NO.:20) CGAGCTGCCTCTTTTTGAAC (SEQ ID NO.:21) FAT 10 AATGCTTCCTGCCTCTGTGT (SEQ ID NO.:22) ATCACTGGGCTTCACCACTT (SEQ ID NO.:23) EPLIN beta AGAAAGGGGACCCTGACTGT (SEQ ID NO.:24) AAGATCCTCACCGTCCTTGA (SEQ ID NO.:25) T cell AGGAGCTGTGGAAAACATGG (SEQ ID NO.:26) CATAACAGACGGTGGCACAA (SEQ ID NO.:27) receptor gamma P28 alpha ACAGGTGGATGTGTTTCGTG (SEQ ID NO.:28) TTCATCCTCCCCCTTCTTCT (SEQ ID NO.:29) Retinal oxidase GTGGTGGACATCATGACAGC (SEQ ID NO.:30) AGCGGCTCCAAGTCTTGATA (SEQ ID NO.:31) Bene CCAGGCAACAAAAGGAGAGA (SEQ ID NO.:32) TGCCTTCTGTCATTGGGAAT (SEQ ID NO.:33) HIF-2alpha/ CCAGTGCATCATGTGTGTCA (SEQ ID NO.:34) CCCGAAATCCAGAGAGATGA (SEQ ID NO.:35) EPAS-1 L-selectin GTGGCACCTCCTACGTCAAA (SEQ ID NO.:36) TGAATCCTTTCCCTTTATGGTC (SEQ ID NO.:37) RNF GAGGTGCAGTCCAAAAGGAA (SEQ ID NO.:38) TGTGTTGGCGTACAGGTCTTTG (SEQ ID NO.:39) Beta-actin TGTGTTGGCGTACAGGTCTTTG (SEQ ID NO.:40) TGTGTTGGCGTACAGGTCTTTG (SEQ ID NO.:41)

[0048]

2TABLE II Temperature conditions for PCR (in .degree. C.) Product Gene Denaturation Annealing Extension Cycles size IGFBP-3 95 60 72 27 247 Mac-2BP 95 60 72 29 249 beta IG-H3 95 60 72 27 199 PCI 95 60 72 28 249 FAT 10 95 60 72 27 246 EPLIN beta 95 60 72 26 261 T cell receptor 95 60 72 26 252 gamma PA28 alpha 95 60 72 27 250 Retinal oxidase 95 60 72 29 197 Bene 95 60 72 26 265 HIF-2 95 60 72 26 250 alpha/EPAS-1 L-selectin 95 60 72 26 195 RNF 95 60 72 29 200 beta actin 95 60 72 21 275

[0049] RT-PCR assays confirmed altered expression for 43 of 47 genes and showed that 13 upregulated genes were induced by RA much more strongly (5-10 fold or more) than indicated by microarray hybridization; these results are shown in FIGS. 2A and 2B. Time course analysis of changes in RNA levels of these 13 genes (FIG. 2A) showed that many of them increased their expression between days 1 and 4 of RA treatment, in parallel with the loss of clonogenicity (shown in FIG. 1A). Analysis of RA dose-dependence of gene expression (shown in FIG. 2B) showed that almost all of these genes were induced even by the lowest (10 nM) dose of RA that produced detectable loss of clonogenicity (shown in FIG. 1B). All 13 genes were also induced by treatment with 1 .mu.M of another retinoid, fenretinide, which is used in breast cancer chemoprevention (these results are shown in FIG. 2C).

[0050] Induction of three genes in this group was tested and confirmed at the protein level, by immunocytochemical assays, shown in FIG. 3A, using rabbit polyclonal antibody against EPLIN (a gift of Dr. David Chang, UCLA), and goat polyclonal antibodies against IGFBP-3 and EPAS-1/HIF-2.alpha. (Santa Cruz Biotechnology, Inc., Santa Cruz, Calif.) and standard techniques. Antibody staining was detected using Vectastain kit (Vector Labs, Burlingame, Calif.) according to the manufacturer's instructions. These results show that induction of IGFBP-3, EPAS-1/HIF2.alpha., and EPLIN mRNA was accompanied by increased expression of the corresponding proteins in RA-treated cells.

[0051] These results showed that RA and fenretinide strongly induced the expression of a common set of genes under the conditions where these retinoids inhibit cell growth and induce the senescent phenotype.

EXAMPLE 2

Biological Functions of Genes Induced in MCF-7 Cells by Treatment With Retinoic Acid

[0052] The genes detected as discussed in Example 1 were found by literature research to have biological functions that are relevant to the cellular effects of retinoids.

[0053] Strikingly, 4 of 13 genes that are strongly induced by retinoids have been reported to possess antiproliferative activity. The first gene encodes insulin-like growth factor binding protein-3 (IGFBP-3), a secreted factor that was shown to be inducible by RA in breast carcinoma cells and to inhibit the growth of these cells (Adamo et al., 1992, Endocrinology 131: 1858-1866; Gucevetal., 1996, Cancer Res. 56: 1545-1550). In addition to its role in IGF sequestration, IGFBP-3 was recently found to bind and modulate the transcriptional activity of a retinoid receptor RXR.alpha. (Liu et al., 2000, J. Biol. Chem. 275: 33607-33613). Induction of IGFBP-3 was confirmed by immunocytochemical assays shown in FIG. 3A.

[0054] Another growth-inhibitory gene induced by treatment with retinoids encodes secreted cell adhesion protein .beta.IG-H3, which is inducible by TGF-.beta. in several cell types (Skonier et al., 1992, DNA Cell Biol. 11: 511-522). Transfection of .beta.IG-H3 was shown to inhibit the tumorigenicity of Chinese hamster ovary cells (Skonier et al., 1994, DNA Cell Biol. 13: 571-584). .beta.IG-H3 is expressed in normal but not in neoplastically transformed human fibroblasts (Schenker & Trueb, 1998, Exp. Cell Res. 239: 161-168), suggesting that this gene may be a tumor suppressor.

[0055] The third gene encodes a LIM domain protein termed EPLIN, an actin-binding protein that is expressed in primary epithelial cells but downregulated in different types of carcinomas (Maul & Chang, 1999, Oncogene 18: 7838-7841). Ectopic expression of EPLIN was shown to suppress cell proliferation in an osteosarcoma cell line (Maul & Chang, Id.). The EPLIN gene encodes two protein isoforns, EPLIN.alpha. and EPLIN.beta., with the larger (.beta.) isoform showing a stronger growth-inhibitory effect. The observed induction of EPLIN gene expression was confirmed by immunocytochemical and immunoblotting assays (results shown in FIGS. 3A and 3B). These assays were performed using rabbit polyclonal antibody against EPLIN (a gift of Dr. David Chang, UCLA) and were carried out by standard techniques. Antibody staining was detected using Vectastain kit (Vector Labs) for immunocytochemistry and horseradish peroxidase-conjugated goat anti-rabbit IgG (Santa Cruz) for immunobloting. Electrophoretic mobility of EPLIN in MCF-7 cells (110 kDa; FIG. 3B) corresponds to the .beta. isoform, consistent with the art that showed stronger growth inhibition by this isoform.

[0056] The fourth gene encodes an ubiquitin-like protein FAT10. FAT10 interacts with a mitotic spindle protein Mad2, and its overexpression in HeLa carcinoma cells was reported to be detrimental to cell survival (Liu et al., 1999, Proc. Natl. Acad. Sci. USA 96: 4313-4318.

[0057] Retinoic acid treatment is known to promote proteasome-mediated degradation of retinoic acid receptor RAR.alpha. (Zhu.et al., 1999, Proc. Natl. Acad. Sci. USA 96:14807-14812) and of cyclin D (Spinella et al., 1999, J. Biol. Chem. 274: 22013-22018). Proteasome-mediated cyclin D degradation has been proposed as a mechanism for retinoid-induced growth arrest (Spinella et al., Id.). Remarkably, one of the RA-induced genes encodes proteasome activator PA28 subunit .alpha.(PA28 .alpha.). Expression of PA28.alpha. is sufficient to activate the proteasome (Groettrup et al., 1996, Nature 381:166-168), and the induction of this gene may account at least in part for proteasome activation by retinoids. Pa28.alpha. therefore can be regarded as another growth inhibitor.

[0058] Still another RA-induced gene encodes retinal oxidase (aldehyde oxidase), an enzyme that catalyses the final step of RA synthesis from vitamin A (Huang et al., 1999, Arch. Biochem. Biophys. 364: 264-272). The observed induction of retinal oxidase suggests that retinoid treatment may stimulate RA synthesis in the treated cells, providing a potential positive feedback mechanism.

[0059] Aside from .beta.IG-H3, two other induced genes encode secreted proteins that may contribute to the senescence-like flattened morphology and increased adhesion of RA- treated MCF-7 cells. One of these encodes Mac-2 binding protein (Mac-2 BP), a cell adhesion factor of the extracellular matrix (Sasaki et al., 1998, EMBO J. 17: 1606-1613). Mac-2 BP is also upregulated in p21-induced senescence of human fibrosarcoma cells (Chang et al., 2000, Proc. Natl. Acad. Sci. USA 97: 4291-4296). The other gene encodes protein C inhibitor (PCI), a non-specific serine protease inhibitor, which is normally produced by the liver. While retinoid-treated MCF-7 cells express markers of senescence, none of the genes that are strongly induced by RA in this cell line has been associated with epithelial cell differentiation. Two of the induced genes, however, encode transmembrane proteins specific for the hematopoietic lineage, including the leukocyte homing receptor L-selectin and T-cell receptor. Induction of these genes correlates with a well-documented differentiating effect of retinoids in hematopoietic malignancies (Warrell, 1997, ibid.). RA also induces another transmembrane protein, Bene, which has no known function.

[0060] The last two RA-induced genes encode known or putative transcriptional regulators. One of them is HIF-2.alpha./EPAS-1, a member of a family of PAS domain transcription factors that mediate the effects of hypoxia and some other stress factors on gene expression (Semenza, 1999, Annu. Rev. Cell. Dev. Biol. 15: 551-578). Interestingly, IGFBP-3 is also one of the hypoxia-stimulated genes (Feldser et al., 1999, Cancer Res. 59: 3915-3918). Induction of HIF-2.alpha./EPAS-1 was confirmed by immunocytochemical assays shown in FIG. 3A.

[0061] The final RA-induced gene encodes a ring finger protein RNF (accession number YO7828). While the RNF function is unknown, it shares 25-38% amino acid identity with a family of regulatory proteins, some of which have been implicated in retinoid response, senescence or differentiation. These include TIF1.alpha., which functions as a ligand-dependent transcriptional coactivator of retinoid receptors (Le Douarin et al., 1995, EMBO J. 14: 2020-2033), as well as promyelocytic leukemia (PML) gene, which is fused with RAR.alpha. in the t(15; 17) translocation in PML (Kakizuka et al., 1991, Cell 66: 663-674). PML has been recently identified as a mediator of accelerated senescence induced by mutant RAS in human fibroblasts (Ferbeyre et al., 2000, Genes Dev. 14: 2015-2027). Another member of the same family is HERF1, which is required for terminal differentiation of erythroid cells (Haradaetal., 1999, Mol. Cell. Biol. 19:3808-3815). Interestingly, HERF1, RNF and FAT10 all map to the major histocompatibility locus on chromosome 6p21.3. This locus also contains the gene for RAR.alpha., which was reported to be induced by RA (Shang et al., 1999, J. Biol. Chem. 274:18005-18010) and to be upregulated in senescent mammary cells (Swisshelm et al., 1994, Cell Growth Differ. 5: 133-141).

[0062] As disclosed herein, retinoid treatment of breast carcinoma cells concurrently induces several genes with known antiproliferative functions, including candidate tumor suppressors that are selectively downregulated in neoplastic cells (EPLIN and .beta.IG-H3). Since the UniGem V array comprises only a fraction of all human genes, the actual number of growth inhibitors that are co-induced by retinoids should be much higher than the genes identified herein. Such additional retinoid-inducible growth inhibitors can be readily identified, however, by hybridizing cDNA probes described herein with larger cDNA arrays or combinations of arrays, or by carrying differential cDNA cloning using methods that are well known in the art (see, for example, International Patent Application, Publication No. WO00/61751, incorporated by reference herein.

[0063] These results demonstrated that retinoids can induce several growth-inhibitory genes, which provide a basis for developing reagents for screening compounds capable of inducing one or more of these genes without producing retinoid-associated resistance or toxicity.

EXAMPLE 3

Construction of Retinoid-regulated Promoter-Reporter Gene Constructs That Are Induced with Retinoic Acid

[0064] In order to produce reporter gene constructs under the transcriptional control of retinoid-induced genes, promoter sequences for all 13 genes that are strongly induced by retinoids, comprising 1400-1500 bp upstream of the 5' end of the longest available cDNA sequence of the respective genes, were identified in the human genome database.

[0065] These sequences were then analyzed for the presence of two closely spaced hexameric core motifs of RARE sites (Mangelsdorf et al., 1994, in THE RETINOIDS: BIOLOGY, CHEMISTRY, AND MEDICINE, (Sporn et al., eds.), pp. 327-330 (Raven Press, New York), in variable orientations, using Regulatory Sequence Analysis Tools, available at: bttp://www.ucmb.ulb.ac.- be/bioinformatics/rsa-tools/.

[0066] A putative RARE site found in only one promoter, ring finger protein RNF, where the sequence:

3 AGGTCACAGCCAGTTCA (SEQ ID No.:42)

[0067] (boldface indicates the RARE core motifs; Mangelsdorf et al., 1994, ibid.) appears in inverse orientation about 360 bp upstream of the apparent transcription start site. None of the other promoters contained discernable RARE sequences, suggesting that most of these genes are induced by retinoids through indirect mechanisms. Interestingly, RNF is also the only gene in this group to reach its maximum expression after just one day of treatment (see FIG. 2A), suggesting that RNF is likely to be directly inducible by retinoids.

[0068] It is remarkable that none of the growth-inhibitory genes that show strong and sustained induction in RA-treated MCF-7 cells contain RARE sites in their promoters, suggesting that it may be possible to induce these growth-inhibitory genes in cells that lack retinoid receptors, and using non-retinoid inducing agents. Reporter gene-containing constructs, under the transcriptional control of a promoter from a retinoid-induced gene, particularly a gene lacking a RARE sequence in the promoter, enable screening of test compounds for the capacity to induce gene expression from these genes in a way that mimics the gene-inducing effects of retinoids without producing toxicity or development of resistance.

[0069] Such reporter gene constructs are prepared as follows. The promoter region of a retinoid-regulated gene, such as .beta.IG-H3, is identified in the genomic sequence (NCBl accession number AC004503 ) as adjacent to the 5' end of the cDNA. Polymerase chain reaction (PCR) amplification of the promoter-specific DNA is performed using genomic DNA from human MCF-7 cells as the template and the following primers for .beta.IG-H3:

4 (SEQ ID NO.:43) 5' GGCCAGGTGCCTCTTCTTAG 3' (sense) and (SEQ ID NO.:44) 5' CGGCTCCAGGGAAGTGAG 3' (antisense)

[0070] using PfuTurbo DNA Polymerase (Stratagene) and 28 cycles of PCR where each cycle consisted of 45 sec. at 95.degree. C., 1 min 30 sec. at 60.degree. C., and 2 min. at 72.degree. C. A 1020 bp fragment is amplified using this method and cloned into the TOPO TA cloning vector pCRII/TOPO (Invitrogen). The sequence identity of this construct is verified, and the HindIII-Xho I fragment containing the promoter in the correct orientation is then inserted into the HindIII and Xho I sites in a firefly luciferase-reporter vector pGL2-Basic (Promega, Madison, Wis.) using standard recombinant genetic techniques (Sambrook et al., 1990, MOLECULAR CLONING: A LABORATORY MANUAL, Cold Spring Harbor Laboratory Press: New York).

[0071] The ability of this construct to drive retinoid-inducible luciferase expression in mammalian cells is demonstrated in transient transfection assays, as described in U.S. Provisional Patent Application Ser. No. 60/______, filed Feb. 1, 2001 (Attorney Docket No. 99,216-E) and U.S. patent application Ser. No.09/______, filed May 21, 2001 (Attorney Docket No.99,216-F), incorporated by reference herein. Briefly, transfection is carried out using LIPOFECTAMINE 2000 (Life Technologies, Inc. Gaithersburg). Cells are plated at a density of 70,000 cells/well in 12 well plates in 1 mL. media containing 2 mM glutamine, 10% FBS, 0.1 mM NEAA (Non-Essential Amino Acids, GIBCO), 1 mM sodium pyruvate, and 10 g/mL insulin, and without penicillin/streptomycin. After culturing the cells for a sufficient time that they attached to the culture dish, transfection was performed in triplicate according to the manufacturer's instructions, using 1 .mu.g pGL2-basic vector DNA and 1 .mu.g pGL2-.beta.IG-H3 promoter DNA. After 10 hours, culture media is replaced with media containing penicillin/streptomycin at standard tissue culture concentrations. The cells were then incubated in the presence or absence of 100 nM atRA for 72 hours. After incubation, cells are washed twice with phosphate-buffered saline and collected in 100 .mu.L of Reporter Lysis Buffer (Promega ). The lysate is left at room temperature for 10 minutes followed by 1 cycle of freeze/thaw using a dry ice-ethanol bath for freezing the cell sample and thawing in a 37.degree. C. water bath. 50 .mu.L aliquots are transferred to fresh tubes for Firefly Luciferase Assay (Promega). Luciferase activity is measured as described above using a Turner 20/20 luminometer at 47.9% sensitivity with a 5 sec. delay period and 15 sec. integration time. An additional aliquot is removed from the cell lysate to measure protein concentration using Bio-Rad protein assay kit (Bradford assay). Luciferase activity for each sample is normalized to protein content and expressed as luciferase activity/.mu.g protein. All assays are carried out in triplicate and displayed as a mean and standard deviation.

[0072] To develop a stably transfected cell line with retinoid-regulated luciferase expression, the construct described above is introduced into a cell line that is susceptible to growth inhibition by retinoids, such as MCF7 cells by cotransfection with a vector encoding a selectable marker, such as pBabePuro, carrying puromycin N-acetyltransferaseas a selectable marker. Transfection is carried out using LIPOFECTAMINE 2000 (Life Technologies, Inc., Gaithersburg, Md.), using a 10:1 ratio of the construct and a plasmid or other vector containing a selectable marker. Stable transfectants are selected using an appropriate amount of a selecting agent specific for the selectable marker encoded by the plasmid or vector. Selective agent-resistant cell lines are isolated and tested for luciferase activity (using a Luciferase Assay System, Promega), in the presence and in the absence of 100 nM RA, or another retinoid at a concentration that produces growth inhibition in the recipient cell line.

[0073] This assay is performed as follows. Cells are plated at a density of 40,000 cells/well in 12 well plates in 1 mL of media containing penicillin/streptomycin, glutamine and 10% fetal calf serum (FCS). After attachment, cells are treated with 100 nM RA or left untreated for different periods of time. Cells are washed twice with phosphate-buffered saline and collected in 300 .mu.L of Reporter Lysis Buffer (RLB; Promega). The lysate is centrifuged briefly at 10,000 g to pellet debris, and 50 .mu.L aliquots are transferred to fresh tubes for use in the Firefly Luciferase assay (Promega). Luciferase activity is measured using a Turner 20/20 luminometer at 55.6% sensitivity with a 5 second delay period and 15 second integration time. An additional aliquot is removed from the cell lysate to measure protein concentration using the Bio-Rad protein assay kit (Bradford assay). Luciferase activity for each sample is normalized to protein content and expressed as luciferase activity/.mu.g protein. All assays are carried out in triplicate and displayed as a mean and standard deviation.

[0074] Such constructs and cells provide a basis for a screening assay for identifying compounds that induce retinoid-induced gene expression. The same type of screening can also be conducted using transient transfection assays with promoter constructs of retinoid-inducible genes rather than stably-transfected cell lines. The methods for high-throughput screening based on luciferase expression are well known in the art (see Storz et al., 1999, Analyt. Biochem. 276: 97-104 for a recent example of a transient transfection-based assay and Roos et al., 2000, Virology 273: 307-315 for an example of screening based on a stably transfected cell line). Compounds identified using these cells and assays are in turn useful for developing therapeutic agents that can induce gene expression of retinoid-inducible genes without the concomitant toxicity or tendency to produce retinoid resistance.

[0075] The absence of retinoid responsive elements in the promoters of almost all of the genes shown herein to be induced by retinoid treatment of MCF-7 cells also suggests that compounds other than retinoids can be screened for their capacity to induce retinoid-inducible gene expression in the absence of retinoids or in cells lacking retinoid receptors. Such screening assays are performed as follows. A recombinant expression construct, prepared as described above and verified for inducibility by retinoids, is introduced by transfection into any mammalian cell line, whether sensitive or insensitive to retinoids, for example HT1080 cells (A.T.C.C. Accession No. CCL121) that are known to lack retinoic acid receptors. Stable transfectants are selected as described above using an appropriate amount of a selecting agent specific for the selectable marker encoded by the plasmid or vector, and selective agent-resistant cell lines are isolated thereby.

[0076] These cells are used in luciferase activity assays (using a Luciferase Assay System, Promega) as described above. These assays are performed on cells cultured in the presence or absence of increasing amounts of the compound to be tested for different periods of time, or cultured in compound-free media.

[0077] This assay is performed substantially as described above. Cells are plated at a density of 40,000 cells/well in 12 well plates in 1 mL of media containing penicillin/streptomycin, glutamine and 10% fetal calf serum (FCS). After attachment, cells are treated with increasing concentration of a compound to be tested, or left untreated for different periods of time. Cells are washed with PBS, collected in 300 .mu.L RLB, centrifuged briefly to remove debris, and then assayed as above using the Firefly Luciferase assay. Protein concentrations are determined to normalize the luciferase results, which are expressed as luciferase activity/.mu.g protein. All assays are carried out in triplicate and displayed as a mean and standard deviation. Finally, cells are assayed in parallel for growth inhibition by the tested compound using cell counting or measuring cell number after staining with MTT (3-(4,5-dimethylthiazol-- 2-yl)-2,5-diphenyltetrazolium bromide), methylene blue or other cell-specific stain

[0078] It should be understood that the foregoing disclosure emphasizes certain specific embodiments of the invention and that all modifications or alternatives equivalent thereto are within the spirit and scope of the invention as set forth in the appended claims.

Sequence CWU 1

1

44 1 1806 DNA Homo sapiens misc_feature IGFBP-3 NCBI acc. number M35878.1 1 ggggattcgt tttgtttcct tcaattttcc aatgaaatca gagatcctgt tcttgggtgt 60 caacgcagat actagaagga ggtgatacaa gagaaaggaa cagcaagcga cgattatggc 120 acggtttcct gtaaacaagg ttgagtgtag ccacagcctg agcactgtgg gagaagagct 180 cataagaaaa tgacggtgct gggccttcgt caccccgggg ccctccattg ttcttgtctt 240 tggtctcttt ttatttgtag aggtccaatt atttatttat ttagtacaag agggaacgaa 300 attgatcttt ccattctaaa aggagagtat atatgtataa aaggaagctg tatagatatg 360 ggggaagagg tggacagggg gaaaagggga gaggacgaga gagagaaagg gagggagagg 420 gacaaggaga gacactgggc gagagatcga ttaggagaga cagaaatgat gaatgaagat 480 taacttcacc caaggcttcg tcgctggagg ggaatggagg agctcctgat ttgctattac 540 tactccaaac tgcaaagggc tccttcaagt cacctatcca cctcctaagg caagcgtcca 600 atttcaacag cgttcaggaa agtctcctcc cgcggaggtc tcaccgcttc ccactccacc 660 cccacaaact ctttggaaaa gtgccttgaa aaatttaatc ctcaatccaa tcctggacca 720 ccagcgtcct ctgttggtca ccgaaggagg gggtgcgcag acaaaactga agaaactcga 780 gtgccagaga aggccgacag gagttacagc gacctcagcg cgcaattgcg ccccgaactt 840 tactgaaaag tgtttagatt gcagagataa gctagaatcc caacgcatcg agaatacagt 900 aatacgaagt cgccttcaaa aaatgacaat gaaaattgcc tattaaagga ctatttggtt 960 aattacgttt cagcagtgcc cagtttattg tctttattat tcttttgtcg tgggtgtaaa 1020 ctccatttga aaacataatc agggagaata cccaagacaa gaagaacagt tgtcatttaa 1080 aatatttgaa aagccctgcc ttaaggagca ttcgcttgcc ggtccactct taattgggga 1140 cttgcggtgt agcaacacgt gagagtcttc ttgcgttgag aagtaagcct ggaaaggcga 1200 aggccccggg gcatcttcag atgcgtattt gtgggcccct ggggatataa acagcccagc 1260 gggtgtaaat taaaccccgc agtgccttgg ctccctgaga cccaaatgta agtcagaaat 1320 gtcccaagac ttcgcctgcc aacggaatta aattttagaa agctccacga ggtacacacg 1380 aatgcggagc gctgtatgcc agtttccccg acaccggctc gccgcaggga gacctcaccc 1440 cgagagcgga aggggtaagg gcggcggggt caaggagatc gggggtgctg agttggccag 1500 gagtgactgg ggtgaccggg ggtgctgagg tggcctggag tgccggggtg gccgggcaca 1560 ccttggttct tgtagacgac aaggtgacgg gctccgggcg tgagcacgag gagcaggtgc 1620 ccgggcgagt ctcgagctgc acgcccccga gctcggcccc ggctgctcag ggcgaagcac 1680 gggccccgca gccgtgcctg cgccgacccg cccccctccc aacccccact cctgggcgcg 1740 cgttccgggg cgtgtcctgg gccaccccgg cttctatata cgggccggcg cgcccgggcc 1800 gcccag 1806 2 1553 DNA Homo sapiens misc_feature Beta IG-H3 promoter NCBI acc. number AC004503.1 2 gactcagggt gtcccaaacc actcatctac ctggcaagcc tgcactctgc atgtgcctca 60 ttctgaacat ggcaccatca ctgctgcaat gtccagacca caaacaccct acaatatcct 120 tgactctcct ttctcccctt ctccctgtat acagactcca aattctattg agactattac 180 ctcctacacc cctcacattt gcccagcctt ccccatctct gcctctacca ccatagttca 240 agctctccca tggtcccttc ctggttacct gttcttcttg cctccttaag cctctcatga 300 cactggccat gtcacttgcc tccacccatc acccgctagg ctcttagctg gagtctgggc 360 cctgctacct tcctcccctt cttccctacc cttgactcca cctccctgtg cttcagccaa 420 ccagataact tgagtttcgt gaatgcatgc ctcagtttac ctgattaact cattttcatc 480 tttcaggcct cagagcaggt atcaccctgt cagggccagg tgcctcttct tagctcccaa 540 agccccagct actcttcatg gaacatcatt ggcttgggct acggatcttc ccaaattgga 600 gctttttcac aaagggctta ggtctcactc attctattaa tccatctgtg tctccccagg 660 gctagcagtg ccaagtaact gacaggtgat taatagatgc ttgggtaagt atcacctctt 720 taccatgtga caatttgttt acctgccttg agctcctcca gggcaggact cttgcctttg 780 cagaatctat ctggcaggta ctgttgcaga gatgtttact gaagaaggga atgaattagt 840 accaaggtga ggaccccacc cttccccacg ggctccaaaa gcagcttaga gcccaacaaa 900 acctgcccca catttttggc gtttctgtgg atcacacgat ttactcatct gtctttcaat 960 gagcatgaca ggtggggtgg gggtggaggg attagagatt gaggagctgg ggagggtggt 1020 cagctcctgg ggtgcagaaa caagtctgat gggccatggt gttctgggaa tcagcactgc 1080 ctcccctcac ccctccctgc agtgttttgt agcctcaaga tcagtgaggg aatcttcggg 1140 cccccagcat gcaggaccga agcccccgag acagctgtcc ctcagtccca aggtccccat 1200 ttggaagcag ccacaggagg cctaagggac ctataccctt ggtttgagga agactgtggc 1260 gagggagaga gggagggagg gctggcagtg agggcaaggg ctgggaaaac tgagcacggg 1320 cacagtgcgg gagcgggtgg gtgcccaggg cagccagggg cgcacgggtt gggaggcgcc 1380 aggcggcccg ccctccttgc acgggccggc ccagcttccc cgcccctggc gtccgctccc 1440 tcccgctcgc agcttactta acctggcccg ggcggcggag gcgctctcac ttccctggag 1500 ccgcccgctt gcccgtcggt cgctagctcg ctcggtgcgc gtcgtcccgc tcc 1553 3 840 DNA Homo sapiens misc_feature EPLIN beta promoter NCBI acc. number AH009382.1 3 tggcatattc atcacctgtt ggaaatctct cttctcacac attttcatta acttcttgag 60 gaagtgtaat tacaagcgtt ttcccacgag cagcccctac taataacgca tcaagctgca 120 tgaattccga aaagcttcag aaaacttgtg gtctgaaacc ctactatgct tgaggtacag 180 gaaagaagga tactatcaaa aggcatcatg cagctggcac ggaactggga caagaatttg 240 ggggcaggat ggccaagtgc taggccaatt gacggtctcc aaaccattag cacagctcct 300 attctgaatg gaggagtaaa aacagctgtt ggagaacttg gacgtcatct gcccttgtca 360 aaccgttttc aggattaatg tacttaattc agctttttcc actacaccac acagcctcct 420 gtaaaacacc ctccctgcac acaacttacc cccaaagcac caggaaccta gctggctaga 480 gttgagctta ggaaaaacct gagtggctcc agagtcaaac tgcgataacg ttgagtcaga 540 ggagttaagg acaaagtaga gctgcacaga ggcccacgtc gtgcaagtgc gtgtctcctt 600 cagagaaagg cgtgccgagg tagactaggc cccgggcagc aaaaaccctg tcccgtcgcc 660 agcgcccgca ccgccagagc gactggagca gacgcgagcg ctgggcacgt agccggtggc 720 gcgcacgctc agcccgaggc cgcacgggag gctgtctggc gtgcgcgccc ccgcggcggt 780 gggcggggtc cggggcgggg ccgcaggagc agtaggtgtt agcagcttgg tcgcgacagg 840 4 1200 DNA Homo sapiens misc_feature FAT10 promoter NCBI acc. number AL031983 4 agtagaaacc tacttcaagt tcccataaaa tcatctgctt ttcactttca gtacagtatt 60 taataactta aatgagatat ttcacacttc agtagtaaat acactttttg ttagataatt 120 ttgtccaact gtatgctaat gtaagtgttc tgagcatgtt taaggcaggt taggttaagc 180 tatgatgttt ggtgggttag gtgtattaaa tgcatttctg attttggata ttttcagtgt 240 acaatgggtt tacagggatg taaccccatc ataagtgaag gagcacctgt acttacttca 300 ttaaaatgct gaaacagtaa ataaggtaac atttaataat atgttgtgca gttcttgaaa 360 tttaagtact caccaaatat tacttttcct ttttttgtta tttacttact tttcattcat 420 ttattaattc atttgtgcat ttagtaaaca tttataaatt atttcctgtg cctgacagca 480 tgctggaaca gtgctaaaga tacaagttaa ttaagacaca atcacgaccc ccaagattcc 540 tactcttttc taaagattac agacaagcag acgatgctat tgttgaagaa acatgctctg 600 agaggcattt gaaggaagtg tagaggatag aagatggaca cataacccag gatggggagg 660 aaaagagtta gggaaggctt tttgacgaag atactgttta caccgtgtgt tcttataaat 720 tcatggtggt ggggatagag ttggaggaaa aggcatgctc agtggcgtgg agatggcaga 780 gagattgggg tgttcaagga tatgccggga attcaaggaa cgagaattcc catagacaca 840 gacacagcta gacatagaga tctgcagctt aggtttgggc tgtgggtata gatccaggtg 900 gcttcaacag acaaagatct ttcctgagaa aagggaaaag ttttcaacac agaaagacca 960 tcccatgttt ggaatgaggt ttgcaaatag attgcttgag gagagaagta tgtgatcaga 1020 aagcattctt tgtctattaa ctcctgccca gcaaaagtga aagaaaattc atgggagcat 1080 gcaagaacaa agagcacagc aaagctggac aaacacagca atccaggcag gggatttcca 1140 actcaactct ggtatataag ctgcatgcaa agtccttttt ctgtctctgg tttctggccc 1200 5 1478 DNA Homo sapiens misc_feature PA 28 alpha promoter NCBI acc. number AL136295.2 5 gtggagtggg caccgcgaag ggaaagcgaa agcggtaggg cgctcgcacc cagctccgcc 60 ttcctgggta gtggggcggg gagggctgga ggaggcgggg tcggatggag gaggcggggt 120 cagccctgcc atggaagggg cggtgccgca gaggagggcg gcgggtgagg ctccgcctag 180 cgcgcacagg cgctcttcac ccaccctccc cgcgccgcct cgcgagggcc agcttccagg 240 ctccccacct ccgctggccc ggtccccgcc tcccgcctcc cgccacctgc cgcccgccgc 300 ctgaggacgg cgggtccggg tcctcccatc gccaagccca agccagtctc tccgcgtccc 360 aacctccacc tcccgcctcc caactggctc cccacctgct ctcttcttcc cctgctccgc 420 ctgggacaga taagctttac ttagggctct tccgtctttc cctagagatg gaataagacc 480 tggcctgtct ttcctagcct tgctagcgtg ggcctctggc tctgccgaca tcctgcgcac 540 ctgtagcaac ggcaccagag ctggcagcat ccctatccaa gcccggacca actctctcct 600 ggaccacggc tgcagatgcc attctataac ccactctcac ggagcagcgg gttcatgcct 660 ctctctggct taaaaccctt tctcagctgc ctgttgacct ttgaatgaaa tccaaaagct 720 gtcgcacctg tctccccttt aagtccactt tctactgctt gccccttcat tcattacact 780 tgcaaatatt tgggctattt gtttctcaga tgtgtcatgt tcatttctgc ctcagggcct 840 ttgcccttgc taataatccc tttgcctaaa acacccttac cctgggtgtt gtcattggtt 900 ctcagtttag atgtcacctc ttcagagaga actattttca tcatccttta taaggaacct 960 ccctccaaat ctattacaaa tcatcctgtt ttagtacatt catagcattt atctctctga 1020 tattagccag gtgttttttt ttttttttcc aggataattt actgatttaa accaggtttt 1080 ggcaaacgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt gggggacgga gtctcgctct 1140 gtcgcccagg gtggagtgca gtggcaccat ctaggctcac tgcaacctcc acctccaggg 1200 ttcaagcgat tctcctgctt cagcctacgg agtagctagg actacaggcg cgtcccacca 1260 cgcccggcta attttttgta tttttagtag agacggggtt tcaccatgtt agccaggatg 1320 gactcgatct cctgacctcg tgatccgccc gcctcggcct cccaaagtgc tgggattgca 1380 ggcatgagcc accgcgtctg gccaactttt tctataaagg gccagagagt aaatatttta 1440 ggctttatag gccttacagt gtctgtctac tcaactct 1478 6 3271 DNA Homo sapiens misc_feature Mac-2 BP promoter NCBI acc. number U91729.1 6 aagcctcccg aatagctggg attaaaggcg cctaccacca tgtttggcta attatttgta 60 tttttttgta gacacggggt ttcaccatct tgaccaggct ggtcttgaac tcctgacctc 120 gtgatctacc cacctcagcc tcctgaagtg ctgggtagtt tcttaaaaag gtaaacatat 180 atctaccata tgacccagta atcctgctcc taggtattta cacaaaataa atacttattt 240 tcacacaaag acttgtatcc aaatgtttcc agcagcttta tgcataatag tggaagatgg 300 aatgacccaa atgtccatca gtgcaaacat gtattaacag tggtgttctg tccatacagt 360 gggccgccac ccagcaaacc caggagccag ttactgattg ttgagatagc atggatggat 420 ctcagaagca ctgtggtaag taaaagaagc cacatgcaaa atattaaata ctgtatgatt 480 ccatttagag ggaattctag ggtccaggag tggtgcctca tgcctgtaat cccagcactt 540 tgggaggcag aggcagggcg ggatcacctg agttcagggg ttcgaggcca gcctggccaa 600 tgtggagaaa ccccttctct actaaaaata caaaaattag ctggccgtgg tggtgggcgc 660 acctgtaatc ccagctactc gggaggctga ggcaggagaa tcacttggac ctgagaggca 720 gagattgcag tgagccgaga ttgttccact gcactccagc ctgggcaatg gagggagact 780 gtgtcttaaa aaagaagaca aaatagaggg aattctagga aaggcaacca gcagtggcag 840 aagctgagag gtggttgctg ggaaggggct gggggaggtg gtggctgcag aggggbataa 900 gagaattctt aggggtgatt gaaacgccct aggtaatgat tgttgtcatg ataccatcgc 960 tacacatttg ccaaaacttt gcacgtaaat tatatgccaa gaaagccaat ttttaaaaag 1020 aaggaaagga tgggtttgaa accccagttc ttcccctacc agctgcacaa ctttagccga 1080 ttacgtcgcc tcactgagcc tctgttttct catctgtaac agggaatata agagcagctg 1140 cttcccatca tggctggaag tattaaatgc attcatttgt ggcaaggctt atagtaatgc 1200 ctggcgaaat ccatattagc tattataggg agcgttcctc aatttgcgga gaggtttggg 1260 gtagaggcac aaaagatgac cttacaggcc agttaaccat tctcatctct gaaatgcccc 1320 gcactttccc ttccatgtct tgggagcggc ttcctgatga cagcagttct gtccacacga 1380 atctgaggct ttcacccagc tgtcttctca gagccgagcc gctgcccctt cccctgcctg 1440 tcccctgtca gcgcttccct ccaccccatg gtcatcgcac accggaaagg ccttgcgagc 1500 cccaggggag cagatgktyg gtgctccgat tccacgagga ggcctctggg ttttccattt 1560 tacctgcctg gatggcttag gactttcccg gactctgggg ctaaagattc ggcacctgag 1620 ttttaaaacc tttcccagca cttcccagag atgccctccc gtcctctgca ctcctgtcct 1680 tccctggcca cttgggcaga agtcattagc actgctgaga agggatgatg ctggggtttc 1740 tgtgcactca ggcccttaat ccggatgaga tttttttaaa ctccccacag ccagttctat 1800 ttccagctgc acctgcccct ggatcttcac aagttcctct ggaggggatt aggcaaaccg 1860 tgcagctgcc taaaacctca caccttgaag gaaatagtca ttgaatgtct gacctctggg 1920 ctggctgtct cggactctaa gctgccaggg aaccagggcc ttccacccag tgggactgcc 1980 tgggggcttt taaatgcccc tgcctgtccc ctactcccag agatggtgac ttcctgggtc 2040 taggcattag gagtttgtaa aactccctga tgattccttc tgtccagccc aggctgagaa 2100 ccactggtca gaggcctggg cacatcccaa ggctcatcca gaaccatggg gtgcaagtga 2160 cagaaacaag agcggctgct gattgcctca ctgagcagtg aagcccagcc ttgaccatgg 2220 attaggccag ctggacccag gagctcaggc cggaggatgc ctgcttccct ctgctctgcc 2280 ccaccggccc cagcagcctg ggcccacatc ctctcagtca gaagctggct ctcaccggct 2340 ggctgggctc acagccccac cctgaaacca gcagtgtggc ccggggcccc cgcaggctca 2400 gacagccagg ccttgggtgg ttgaaggcca agagctgggg gccctctggg aaccacacag 2460 ccgggaatgg gagggggtgc tccccaaggg acagttgagg tgccggcttt cagtgggagg 2520 aaagggaatg ggtatgagct ggacagagcc attatgtcac ccagagaggc tctgtccccc 2580 gccccgctga gggggagaca gtaggagagt ggccacaggt ccagcagtgg cgagcacagg 2640 ctctggggtc aggtgttgga gcagggtcca gctcctccac tggccagctg catacctggt 2700 tctcagtgcc tccctcccct ggggacaggg gacagtgcca tgcaaccttg tggggcacag 2760 gccctctgtg tggtcagcat gccaagagca cagagagggt ggatttgcac atgagcagcc 2820 ccctgtgtgg tgttcaccca gccagcaacg tgctagaccc aggaaaagac tcggagcgct 2880 ctgtcagagt ccacagccac accaccaggt gcagactgtc tgggcccaga gcctctgctt 2940 cttcccctcc cgtccaccaa acgccagccc ctgaccacct ggcggccttt ccaactgagt 3000 gtggctgtta gtcctcttgc aggccttgct ccagccagac tcccaccttg ggcctctgcc 3060 agcctggcac tgatagccac aggcagagct gagacaaaag agaggggccc tggggagtat 3120 cagcagcagc caatcccgga agacatctat gtcaggtggt ttctggaaat cgaaagtaga 3180 ctcttttctg aagcatttcc tgggatcagc ctgaccacgc tccatactgg gagaggcttc 3240 tgggtcaaag gaccagtctg cagagggatc c 3271 7 1500 DNA Homo sapiens misc_feature PCI promoter NCBI acc. number AL049839.3 7 ctgccatgcc tactgctcac acttccatag cacgtgcccc caagcacccc atggtgtagg 60 tgctgttatt atcactatct tacagttatg gagcagtggc tcaaggtgta actgacttgc 120 ccaaaatcac actacaagga cacagcaggg ctgagatttg aacccaggca gtggcttcag 180 agcctgagct gtttcctact gcagagggag gaggcaagac ttctacccgt agccagatgg 240 ggaggcatgg gcacaggaac ggctcttggg tgaagtggag ggaggaagag gaggactgaa 300 ggccaaggcc acgtcaggag tgatgggaga ccccacaaag gcctccctga gaagagctag 360 agacaaagat gagtgcctcc tcatctggaa gatgaaaaga tgtctttgcc tgcatgggct 420 gctgtcacaa agtcccaggg gctagggggc ttcaacaaca gaaatttctt tctttacaac 480 tctggaagct ggaagtctga gattaaggca ccagcaggat ttgttccttc caaggcccct 540 ctccttggct cacaggtggc tgccttctcc ctgtcttcac ctggtcttcc ctctgtgcat 600 gtctctatcc tgatctcctc tttttaattt ttgtgtaagg acgtagtcat attgggttgg 660 ggcccactct agtgacctca ttctaactca gtcccctctt taaaagccct atctccagat 720 atagtcacat tctggggtat tgaaggtaag gacttcagca tatgcatttt gggggcacaa 780 ttcagccaga acaggaggac ggtggggatg tccacatgaa gaggttcagg cagaattcct 840 ttaggagggg aagatgtctc tctgtgggac aagggtggca tggagcagcc cctgggggaa 900 ggagaagggg acagtttgca tactggtatt ctgcctaccc cagggtggac actcactcag 960 cgtttgctga atgaacaggg caaggccagc agtgctgatg gtcccaggca tgtagctggt 1020 ctgagttcat agaaggacca cagcgccctg ccatgtgcca aaccaggaca ccagagtgaa 1080 ggccagaagc tcacatggaa gcagcttagt tccctggtaa cctcgagatg ctgatgagac 1140 agagcagagc agagggaacc ctctccctcc atatcccatc ctccaaaatg tgtcccttga 1200 tgtggatggg tagacaggat tcctgccctg gcagccagac ccctgccttg ggtctgcacc 1260 tcctctccct ccttcctctc cccgtcatcc ctaaatcttg tcctcgagcc actgccaccc 1320 tgtgtaaacc ctcatgccca gtcttgcggg tgccatccct tctctttgaa gctgaatgga 1380 ccaaacatac ccattgagtg ttgggtgggg acatctctgg aaagtcagca cctggaccag 1440 ctccacccct ctctgaggac accttctttc cctttcagaa caaagaacag ccaccatgca 1500 8 1674 DNA Homo sapiens misc_feature T cell receptor gamma promoter NCBI acc. number AC006033.2 8 cttacgagcc ccaaggactg ccagcgttga tgtgtggagc agtgacagca tgtctgcagg 60 cactgtgctt tctgccaggg cagcctgaaa tcaccgcaga gaagcgttag acctctgtgg 120 cttggctgag tccaccaagt caaacttccc atgggtgagg gtgacatggg gccccctgca 180 tcgttgtaaa gcggtgtcct caccacctgt tagcacttcc agtctgtgtg aagacagtcc 240 tgcaaggtct gcagcctgag atcagaccac atagtttaga cggccacctc agaccacaag 300 gcggccaccc cagcaagttg tcaggctggt gttgttggtt gctggggctg tgacatgcag 360 cattgtcttc tgagagcttg tcttcaacca gctggagaga tgttggtccg tcaagccgct 420 tagctctgct cagctaccca cacagcctgc agcaccgggt ccttctggtc accttcatga 480 ggctgggccc tccatcccag tttgtttgct tctgttgaag attcaggttc ctgtcccctc 540 cctcagctac ctgaataatg tacatctcct gaatcccggc ttctcctcaa tgacagtttc 600 ctattgtctg ttgttctttc tctaagccaa gacatttaat ccgtcccaag gtatttttac 660 aaactgctct ccccagtgca tcccttaaaa gctgctgtgt tccagatttc catgcttaat 720 ttacccactg ggagctgcag ctcactgcca ctgcccagca tcgcaagaga gtatcataac 780 cttatatcac tgtcctgggg aaacagcaaa ggtcaaatta tgttttctac caaatgcgtg 840 tcacttttgc accatcataa agtaaaaaaa aatcttaagt cggacctcag ttaaatcgaa 900 agctgtctgt acccatatcc agctaactct tggacatttt caagtacgtc tgacatggga 960 tctcaaacaa agtctgctca tagccagagt gaactcattc ccttccccca aaccatatct 1020 tcttccgagt tccctgtatg cataactacc cacattgccc aagccaggag cttgagcatc 1080 agcctcaatt ctcccctcta attcaccgca ctctaattcc tgaggattct acgtcctaaa 1140 catttcctgg ctcaccactg cctccacctc acgcacctcc atacatccca tctcggcccc 1200 tcccacccac cttccccatg gccactagac tgacctggtc cttttcctgc tctaaagtcc 1260 ttgctctctc taccttgcct ctgaagatga agtccagagt tcttaggata agaggttctc 1320 tgtgatgtgg ccaccccctc cctgtccttc catcttcatt tagtcacttt ctgcctggaa 1380 ttccacgacc cacttctatg gattgacttg aatttttttg tgtttggact gcattctact 1440 ccacattccc tggctaattc ctgtttatcc ttctgggctc agccccaggc agtcttcccc 1500 aggaagctta cctcacccag taagtccagg atggagatgc ttcaaattgc tttctcttca 1560 cgccacctag tgccttcctc tctcctagca cctcctaccc aagtcttggc ctgtttaccc 1620 atttctctct cattctaaac gacagttaag agttcccagg ggagtaagat catg 1674 9 1510 DNA Homo sapiens misc_feature Retinal oxidase promoter NCBI acc. number AF010260 9 cacctatgaa gtgttcttgc cctctccccc ccaaaaattg aacctgaatt taatcaaagc 60 tttagatctt aatatttagt gcatagggaa ataagtagag tagaaaaaca ataccaggga 120 gaagcaatta gccacattca aaaaatggtt catttcaata gaacaactga cctggtttct 180 ttttcaatgt gttaatagtg ttaaaaagtc tgttttagat gaaaagagac tgatgagacc 240 acatgcaaat acattgcaca gctttgtttg attcaaagaa gtcaagtgta aaagacattt 300 tttagacacg tgaaatggtg ttgcggctga ctaaaaggat gtgtatgtgt gtttttgaag 360 tatttagggc taaaatatgt ccaggattgg cttttaaata ctacaaaaaa tggagtatgc 420 caaaacgttg accattgtta aagctcagtg aagggcaggt agatgccaat tgcactcttc 480 acttttatgt gcaaagtttg gaaaatttca caataaaatt tttgtgttta cataaatgaa 540 ataacatggg aaaatgttct aaggtatggc aagtgaaaag aagcctggaa aataactagt 600 ttgtcccact taagttttta aaggtgttaa aagtacatgt ttcaaaaagt aaggatagac 660 taaaagtgaa cgtggtaagt tctaagttgt gggcttacag gtgttcttta tttttatgct 720 ttgctgtatt ttccaagttt tttttttaag ttttccaaga tgttttacat ctctgttctt 780 atttaccaga taaactttgt ggttagttac tggataaact gtaaatagtg ataaaatttt 840 taagtttata tcaagatagc acttcatttt aaaaccagta attattaggt tggtgcaaaa 900 ttaattgcag

ttgttgccat tggaagtgat ggtaaaaacc gcaattactt ttgcaccaac 960 cttatatttc taaaagatca agttgtaaac ctatttgttt tccctaagat ccgctcttgc 1020 agagttccaa taaatatgat tgtttacact taagagtcca ggactacagc aggcctggtt 1080 ggaggggagt tactaatgtt cccagactta aatccagctg gaacaccacc taaaatatgc 1140 agtaacataa gaccatcaaa agcaatgtcc caggacttac aatgtttgct aagacgcaag 1200 agggtgtgac acagacgcta agcgccactg gcgaggagat gaaggggtcg tcttcatctt 1260 cgccggatga tttccgccca catagagggc gccagtgacg cccacacacg tgctggtgtc 1320 ccgggaagag ttcctggcaa agagctcagg taacgttgga tcttaattca aggctttctc 1380 cgttcggggt ggatgggttg gtactttagg ctccagcaag ccccgcccca ctcggcgggt 1440 cggtgccgcc gggtcccagg tgcccgctac ttcccagaac ctccgcctcc cgctccgggc 1500 cctcgaacca 1510 10 1954 DNA Homo sapiens misc_feature Bene promoter NCBI acc. number AP001234.3 10 caaatatctg gaagataaaa gcataaaaga aggagcttca ttagccagta tagagcatgt 60 ttccctttgc agggcatctc ttttctgtct ctagttaaaa gctcaggtga agttaggagg 120 gaaccaaggg ggaaatggag caggaagcct ggcccctctg agtcatggta aagtcacatc 180 cgattgttag gaaattcaag gggttgaaaa gcatgggcaa ggacttcatg tctaaaacac 240 caaaagcatc agcaacaaaa gccaaaattg agaaatggga tctaattaaa ctaaagagct 300 tctgcacagc aaaagaaaca accatcagag tgaacaggca acctacagaa tgggagaaaa 360 tttttgcaat ctacccatct gacaaagggc taatatccag aatctacaaa gaacttaaac 420 aaatttacaa gaaaaaaatc aaacaacccc atcaaaaagt gggtgaagga tatgaacaga 480 cacttctcaa aagatgacat ttatgcagcc aacagacaca tgaaaaaatg ctcatcatca 540 ctggccatca gagaaatgca aatcaaaacc ataatgagat accatctcac accagttaga 600 atggtgatca ttaaaaagtc aggaaacaac aggtgctgga gaggatgtgg agaaatagga 660 acacttttac actgttggtg ggactgtaaa ctagttcaac cattgtgaaa gacagtgtgg 720 cgattcctca aggattgaga actagaaata ccatttgacc cagccatccc gttactgggg 780 atatacccaa aggattataa atcatgctgc tataaagaca catgcacacg tatgtttatt 840 gttgcactat tcacaatagc aaagacttgg aaccaaccca aatgtccaac aatgatagac 900 tggattaaga aaatgtggca catatacacc gtggaatact atgcagccat aaaaaatgat 960 gagttcacgt cctttgtagg gacatggatg aaactggaaa ccatcattct gagcaaacta 1020 ttgcaaggac agaaaaccaa acactgcatg ttctgactca tagatgggaa ttgaacaatg 1080 agaacacttg gacacagtgt ggggaacacc acacaccagg gcctgttgtg gggtaggggg 1140 aggggggagg gatagcatta ggagatatac ctaatgtaaa tgaggagtta atgggtgcag 1200 cacaccaaca tggcacatgt atacacatga aacaaacctg cactttgtgc acatgtatcc 1260 tagaacttaa agtataataa aaaaataaaa taaaataaat aaaaaataaa aaaagaaatt 1320 caagggttta atgcagaaat cgtgaacaga gggactctcg accaactctg gcctgtgaat 1380 atgtcttgtt ggctcaagca gtattggcat atacactttt aaacaattct gaataagttg 1440 ccaacattta aaacaggata tttcacatgg aaaatccata aattcggtta tattgcttag 1500 tatatacgtc tttggcacgc gattgaaacg cgctaattgc atcagcctat ctttctatgc 1560 aagaatgcaa gaaaaattga tgtgatgtgc cttatcacaa ttcattacct cctatttcct 1620 ctgcagcaac aagtttcctt gattataaag gtctttagcg tgagaggtac aggtgttatg 1680 gcacgtgcga ataagggcag aaattaatca aatttatcaa ctatttggcg atggctcgag 1740 acaggtatag aaccactact aggtgatatt gaggcttttg tacaatttat agcaagtttt 1800 tgagagtccc ttcaagtttg ttacataatc ttctttgtgc aacgtacaag agcaaagtag 1860 aaaaatttgg tttttatttt tttaagcaac atcagctgca ctagttgagc ttttgacaag 1920 acatactgct caaaaaatct tcataacatt attt 1954 11 1520 DNA Homo sapiens misc_feature HIF-2alpha/ EPAS promoter NCBI acc. number NT_005065.3 11 caacttcaag ttacacctgt gaaactcatg ggtccttcca cagccttcaa aaactaaggg 60 cgtcccctgt cctctcccca gatgtccctt ccccatcgcc ggtagcgagt gggagacagc 120 tcagcgcggg gcaggggagc actgggcccg gagatggaag gcagcgtcaa aagcgccgct 180 ggaaaatccc tgagcgctaa ccgttgcctg tgtgagccct taaatctaca aatttccaac 240 acctgtagcc tttgggtttc ccaggacttc catcgaccct ggcggcagag agggcaggcc 300 tgagatgcag tgacttgagg gcacatggcc aactcttgtc actccaagat cacactgggg 360 aaccagactg acttctccaa ttctgaactc gccccggcct cgggcggctc aaagggcctc 420 ctctgccgca tccccgccaa aaccaaaccg cctggcacaa gccggtaagc aaccaccctg 480 ctgggagagg gaaggaagag taggcgcagc cctagatcaa tttccttgca ctgcttctcc 540 cagacggtca agtcagctgc gtcccaccga aaagggcgca tcgcccacgc ccgaaacgca 600 gccgctgggg gccgagaaat tatccccacc tggcccgagg gccagggacg caggagcgca 660 gcagcgtgga ggggctccgc gctggcccgg cgctgcccgc ggtcctgccc tcgttccaag 720 ggcacggcgc cggtacgagg acaccgacgc tgtggcgcaa ctgccgtccc ccgcagcaat 780 cccggagccc ggctcccggc cgcccctcgg ccctgcgcag gctgcctctc cccgacgcgg 840 agtcccaccc cgctacccgc cgcccagacg acctcataaa caagtcctcg aagtgcggag 900 gcaggaggcg gggcgcagcg cgggggcagg aggcgggcca gggtcagggc agaggctgcg 960 gccgcgcgtc cccattggcc gggacgcagt gagccgcccg gagctcggcg cgggcggggc 1020 ctgccggcgc gtgcccgccc acacacccgc gccggtgccc gccccccgcc ctccgcgccc 1080 gccccgtgcc cgccccaagc cggccgacgg agtttttaaa gtgggctgcc ggccgcggga 1140 gctttacact cgcgagcgga ccgccacacg ggtccggtgc ccgctgcgct tccgccccag 1200 cgctcctgag gcggccgtac aatcctcggc agtgtcctga gactgtatgg tcagctcagc 1260 ccggcctccg actccttccg actcccagca ttcgagccac tttttttttt ctttgaaaac 1320 tcagaaaagt gactcctttt ccagggaaaa aggaacttgg gttcccttct ctccgtcctc 1380 ttttcgggtc tgacagcctc cacccactcc ttccccggac cccgcctccg cgcgcaggtt 1440 cctcccagtc acctttctcc acccccgccc ccgcacctag cccgccgcgc gccaccttcc 1500 acctgactgc gcggggcgct 1520 12 1469 DNA Homo sapiens misc_feature Selectin promoter NCBI acc. number AL021940.1 12 catggctttg cttggtcctt ctctagttct tctgcagccc attgagcctc ttgacttagc 60 acaagggtct caggtccttg cccaaaggga gtgtgctgtg ctgcaggtag actgcactga 120 atgtcaacag aaagccttgc tttctttcat ttctctaacc cagtctcaca tcctcctcct 180 cctccccttt tccctcccct tcctcctgca cttctctttc ctctttcccc acccctttcc 240 tagactggcc tctattgcct cccactgaga caaaaatgaa ctgctgatca aaagtaatgt 300 gactagattc tctcttcctt ccctcctttc tatccttcct tccattctcc tatgcatctt 360 tccttaccct cctcctcctt cactcattgt tgttgctgtt cttcttcctc ttctttttcc 420 tcctgctcct cttcttctac ttgttcttgt tcttgttttt gtttggttct tgttctcctc 480 ttcctccttc tctctctcct cctcctcctt cttttccacc accctcccct atctttttca 540 taaatgctaa actaactctt ggctacctgt ggtaaatggc ccttggaaat tgcaaatact 600 acaaatcaaa actgcatttc agacatattt atgatgtttg caaaacttca gtagagctaa 660 gcagtggact tgactcgttt cggttccttc acctccgtct ttccttgctc accacctagt 720 ggacgtcctt gttagtggca cttcctgaag ttaacccctg aagagagccc atgctctcta 780 gcttttcacc gtgtaggttt gggagcctac aagtaccttt aatattcttg gactataaaa 840 tgagatggtt ttataagact gcatgtgaaa ttaggaccca tatgatgaag gacaataaaa 900 aggaagaccc actgatgtga gtcaatgagt caaatgcaaa tcagatttgc atttttagga 960 aaataataat aacaacaaca aaaactctga agctcagcgc cccatattta ttatattgtt 1020 taatctttat aacagctctc tgctatagat atgattatta tccccattct aaagagtctc 1080 aaagaggtta agaaacaaat tcaaaaacta gcgaaagaca agaaataact aagatcagag 1140 cagaaccata ggaggtagag acacgaaaaa gccttcaaaa aatcaataaa tccaggagct 1200 gcattttgaa aagattaaca aaatagatgg accactagct agactaataa gaaagaagaa 1260 tcaatagaca caataaaaaa tggtaaaggg gatattacca ctgatcccgt agaaatacaa 1320 actaccatca gagattacta taaacatctt tacacaaata aactagaaaa tctagaagaa 1380 atggataaat tcctggacac atacaccctc ccaagactaa accaggaaga agtcaaatcc 1440 ctgaatagac taataacaag ttctgaaat 1469 13 1490 DNA Homo sapiens misc_feature Ring finger protein RNF promoter acc. number AP000518.1 13 taaatcatca tgccagtcaa acaaaacctg tcaaaagtta aattctgctt ctaggggcca 60 gcagtttagg agtataagta aaacaattta cagatgttag actgttttaa tttaacccta 120 aaacaaacaa aaagaaaggt ctggaggtat aacatttctg aaagtctttg gtttacagca 180 gttgctataa ggggagccac ataatttata gtccaaactg gacatttctg aaagtgaaag 240 gaggtgctat taataattac accaggacaa agtgaaaccc aggatggttc caggcaaagc 300 agagtgtatg atcactctgg ctattattat aataatcatc cacaagccct gtttgaccta 360 agattaagat cagacaaaaa ttaatggtgt acttcttgct ggggacagac ggctgataat 420 ggagagtgag gaggtgaggg tggaagctat accaagagaa ggggtaggga ggaagcaccc 480 ttttccttaa gacaagaggc aaggagggaa ggttaggaca tgaatgtaca gaagggaatg 540 tatgtaacac tggttgatat attcctagtc ataacaaaag ccatagaagg caagtcaggg 600 atcagagaag caccaagaag gaagaagaag aacatataga cagaattggc aaagcaaaga 660 atgggcacgg agacaccagc atactggaga catacagaga aaaaatcaac agaggacaga 720 ctactacagg tgttgtgggg aggcagaaga tcaccagggg caagagcaaa gtgcaaaaac 780 aaagaacaac tctttagaaa ggaagttcct tgcctatcct actgagctag agagtggttg 840 gttgaccctg tgactggaaa ttccccaagg taggtgatga taacctctac attttcacaa 900 aattctgtga ggagccaaag cacctgaggt agagaattgc ccttccccta ctttccagat 960 gctctaccaa ggcttgaact ttgcatacaa gatgcccaaa gcattgcagt gaactggctg 1020 tgacctttca gtaggcatca ccacccaccc ctccactccc tactcagagc tgattgggaa 1080 atcccccata agtggtgttt ggtgccccgg tcattctgat tttagtcaac caccatacaa 1140 acataccttt agtccaaagt tcaggacaac ttatttcact ttataagcag cctattacac 1200 attcaaagta tccatttgtt ctcaagaggt agcaaggtag gactgcccat ctgttttcct 1260 ctctttataa tattttctag atcctaaatt ttacgctttt ctatcattta tttttttctc 1320 cctcttcttt tcctctctct ctgctcttct aactaattgg cagaatctct gacctccact 1380 ttctctgact cccttctccc ttcctagaaa cagtatccac agtggactcc ggggctccta 1440 cagacttggc acagcttcct acagtcttga aacagccctg ttgttctgtc 1490 14 20 DNA Homo sapiens misc_feature Sense primer for IGFBP-3 14 ttgcacaaaa gactgccaag 20 15 20 DNA Homo sapiens misc_feature Antisense primer for IGFBP-3 15 catgaagtct gggtgctgtg 20 16 20 DNA Homo sapiens misc_feature Sense primer for Mac-2 BP 16 aattccacac tgtgcccttc 20 17 20 DNA Homo sapiens misc_feature Antisense primer for Mac-2 BP 17 gtggagtctg gaaggactgg 20 18 20 DNA Homo sapiens misc_feature Sense primer for beta IG-H3 18 tgcgactagc ccctgtctat 20 19 20 DNA Homo sapiens misc_feature Antisense primer for beta IG-H3 19 catgcacaag gctcacatct 20 20 20 DNA Homo sapiens misc_feature Sense primer for PCI 20 gcacccaaga gcaagacttc 20 21 20 DNA Homo sapiens misc_feature Antisense primer for PCI 21 cgagctgcct ctttttgaac 20 22 20 DNA Homo sapiens misc_feature Sense primer for FAT 10 22 aatgcttcct gcctctgtgt 20 23 20 DNA Homo sapiens misc_feature Antisense primer for FAT 10 23 atcactgggc ttcaccactt 20 24 20 DNA Homo sapiens misc_feature Sense primer for EPLIN beta 24 agaaagggga ccctgactgt 20 25 20 DNA Homo sapiens misc_feature Antisense primer for EPLIN beta 25 aagatcctca ccgtccttga 20 26 20 DNA Homo sapiens misc_feature Sense primer for T cell receptor gamma 26 aggagctgtg gaaaacatgg 20 27 20 DNA Homo sapiens misc_feature Antisense primer for T cell receptor gamma 27 cataacagac ggtggcacaa 20 28 20 DNA Homo sapiens misc_feature Sense primer for P28 alpha 28 acaggtggat gtgtttcgtg 20 29 20 DNA Homo sapiens misc_feature Antisense primer for P28 alpha 29 ttcatcctcc cccttcttct 20 30 20 DNA Homo sapiens misc_feature Sense primer for Retinal oxidase 30 gtggtggaca tcatgacagc 20 31 20 DNA Homo sapiens misc_feature Antisense primer for Retinal oxidase 31 agcggctcca agtcttgata 20 32 20 DNA Homo sapiens misc_feature Sense primer for Bene 32 ccaggcaaca aaaggagaga 20 33 20 DNA Homo sapiens misc_feature Antisense primer for Bene 33 tgccttctgt cattgggaat 20 34 20 DNA Homo sapiens misc_feature Sense primer for HIF-2alpha/ EPAS-1 34 ccagtgcatc atgtgtgtca 20 35 20 DNA Homo sapiens misc_feature Antisense primer for HIF-2alpha/ EPAS-1 35 cccgaaatcc agagagatga 20 36 20 DNA Homo sapiens misc_feature Sense primer for Selectin 36 gtggcacctc ctacgtcaaa 20 37 22 DNA Homo sapiens misc_feature Antisense primer for Selectin 37 tgaatccttt ccctttatgg tc 22 38 20 DNA Homo sapiens misc_feature Sense primer for ring finger protein RNF 38 gaggtgcagt ccaaaaggaa 20 39 22 DNA Homo sapiens misc_feature Antisense primer for ring finger protein RNF 39 tgtgttggcg tacaggtctt tg 22 40 22 DNA Homo sapiens misc_feature Sense primer for Beta-actin 40 tgtgttggcg tacaggtctt tg 22 41 22 DNA Homo sapiens misc_feature Antisense primer for Beta-actin 41 tgtgttggcg tacaggtctt tg 22 42 17 DNA Homo sapiens misc_feature RARE sequence from ring finger protein RNF promoter 42 aggtcacagc cagttca 17 43 20 DNA Homo sapiens misc_feature Sense primer for beta-IG-H3 reporter gene construction 43 ggccaggtgc ctcttcttag 20 44 18 DNA Homo sapiens misc_feature Antisense primer for beta-IG-H3 reporter gene construction 44 cggctccagg gaagtgag 18

Claims

We claim:

1. A method for identifying a non-retinoid compound that induces expression of a retinoid-inducible gene in a mammalian cell, comprising the steps of: (a) culturing the cell in the presence and absence of the compound; (b) assaying the cell for changes in expression of at least one cellular gene whose expression is induced by a retinoid wherein the promoter does not contain a RARE site; and (c) identifying the compound as an inducer of retinoid-induced gene expression if expression of the cellular genes of subpart (b) is higher in the presence of the compound.

2. The method of claim 1, wherein the cellular gene is insulin-like growth factor binding protein-3 (IGFBP-3), secreted cell adhesion protein .beta.IG-H3, epithelial protein lost in neoplasm (EPLIN), ubiquitin-like protein FAT10, Mac-2 binding protein (Mac-2 BP), Protein C inhibitor (PCI), T cell receptor gamma, retinal oxidase, Bene, HIF-2alpha/EPAS- 1, selectin L, or proteasome activator PA28 subunit .alpha.(PA28.alpha.).

3. The method of claim 1, wherein the cellular gene is a gene wherein expression thereof in a mammalian cell is induced by a retinoid and inhibits growth of the cell thereby.

4. The method of claim 1, wherein the cellular gene is human insulin-like growth factor binding protein-3 (IGFBP-3), secreted cell adhesion protein .beta.IG-H3, epithelial protein lost in neoplasm (EPLIN), ubiquitin-like protein FAT10 or proteasome activator PA28 subunit .alpha.(PA28.alpha.).

5. The method of claim 1, wherein expression of the cellular gene is detected using an immunological reagent.

6. The method of claim 1, wherein expression of the cellular gene is detected by assaying for an activity of the cellular gene product, wherein the cellular gene is insulin-like growth factor binding protein-3 (IGFBP-3), secreted cell adhesion protein .beta.IG-H3, epithelial protein lost in neoplasm (EPLIN), ubiquitin-like protein FAT10, Mac-2 binding protein (Mac-2 BP), Protein C inhibitor (PCI), T cell receptor gamma, retinal oxidase, Bene, HIF-2alpha/EPAS-1, selectin L, or proteasome activator PA28 subunit .alpha.(PA28.alpha.).

7. The method of claim 1, where expression of the cellular gene is detected by hybridization to a complementary nucleic acid.