U.S. patent application number 11/119096 was filed with the patent office on 2005-09-01 for human methionine synthase reductase: cloning, and methods for evaluating risk of neural tube deffects, cardiovascular disease, cancer and down's syndrome.
Invention is credited to Gravel, Roy A., Leclerc, Daniel, Rosenblatt, David, Rozen, Rima, Wilson, Aaron.
Application Number | 20050191701 11/119096 |
Document ID | / |
Family ID | 34891279 |
Filed Date | 2005-09-01 |
United States Patent
Application |
20050191701 |
Kind Code |
A1 |
Gravel, Roy A. ; et
al. |
September 1, 2005 |
Human methionine synthase reductase: cloning, and methods for
evaluating risk of neural tube deffects, cardiovascular disease,
cancer and Down's Syndrome
Abstract
The invention features a novel gene encoding methionine synthase
reductase. The invention also features a method for detecting an
increased likelihood of hyperhomocysteinemia and, in turn, an
increased or decreased likelihood of neural tube defects,
cardiovascular disease, Down's Syndrome or cancer. The invention
also features therapeutic methods for treating and/or reducing the
risk of cardiovascular disease, Down's Syndrome, cancer, or neural
tube defects. Also provided are the sequences of the human
methionine synthase reductase gene and protein and compounds and
kits for performing the methods of the invention.
Inventors: |
Gravel, Roy A.; (Calgary,
CA) ; Rozen, Rima; (Montreal West, CA) ;
Leclerc, Daniel; (Montreal, CA) ; Wilson, Aaron;
(Calgary, CA) ; Rosenblatt, David; (Montreal,
CA) |
Correspondence
Address: |
CLARK & ELBING LLP
101 FEDERAL STREET
BOSTON
MA
02110
US
|
Family ID: |
34891279 |
Appl. No.: |
11/119096 |
Filed: |
April 29, 2005 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11119096 |
Apr 29, 2005 |
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09487841 |
Jan 19, 2000 |
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09487841 |
Jan 19, 2000 |
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09371347 |
Aug 10, 1999 |
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09371347 |
Aug 10, 1999 |
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09232028 |
Jan 15, 1999 |
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60071622 |
Jan 16, 1998 |
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Current U.S.
Class: |
435/6.14 |
Current CPC
Class: |
C12N 9/1007
20130101 |
Class at
Publication: |
435/006 |
International
Class: |
C12Q 001/68 |
Claims
What is claimed is:
1. A method for detecting an increased risk of cancer in a mammal,
said method comprising detecting the presence of a homozygous
methionine synthase reductase (MTRR) polymorphism in said mammal,
wherein detection of said polymorphism indicates said mammal has an
increased risk of developing cancer.
2. The method of claim 1, wherein said polymorphic MTRR is detected
by analyzing nucleic acid from said mammal.
3. The method of claim 2, wherein said nucleic acid is genomic
deoxyribonucleic acid (DNA).
4. The method of claim 2, wherein said nucleic acid is
complementary DNA (cDNA).
5. The method of claim 1, wherein said polymorphism is selected
from the group consisting of: (a) a G instead of an A at position
66 relative to the first nucleotide of the start codon of MTRR, (b)
a G instead of an A at position 110 relative to the first
nucleotide of the start codon of MTRR, (c) a deletion of 4
nucleotides starting from position 1675 (nucleotides 1675-1678)
relative to the first nucleotide of the start codon of MTRR, and
(d) a deletion of 3 nucleotides starting from nucleotide 1726
(nucleotides 1726-1728) relative to the first nucleotide of the
start codon of MTRR.
6. The method of claim 2, wherein said polymorphic MTRR is detected
by a method comprising: a) PCR-amplifying a segment of MTRR nucleic
acid from said future female parent, said embryo, or said fetus
using primers MSG108S (SEQ ID NO: 49) and AD292 (SEQ ID NO: 50),
and b) digesting the product of the PCR amplification reaction with
the restriction enzyme Nde I, wherein a PCR product that is
digested by Nde I indicates the presence of said polymorphic
MTRR.
7. The method of claim 1, wherein said polymorphic MTRR is detected
by analyzing MTRR polypeptide from said mammal.
8. The method of claim 1, said method further comprising detecting
the presence of a polymorphic methylenetetrahydrofolate reductase
(MTHFR) in said mammal, wherein detection of said polymorphic MTHFR
indicates an increased risk of developing cancer.
9. The method of claim 8, wherein said polymorphic MTHFR has a T
instead of a C at a nucleotide position equivalent to position 677
of SEQ ID NO: 51.
10. The method of claim 8, wherein said polymorphic MTHFR is
detected by analyzing nucleic acid from said mammal.
11. The method of claim 8, wherein said polymorphic MTHFR is
detected by analyzing MTHFR polypeptide from said mammal.
12. The method of claim 1, wherein said polymorphic MTRR contains a
methionine instead of an isoleucine at amino acid position 22.
13. The method of claim 1, wherein said cancer is colon cancer.
14. The method of claim 1, wherein said mammal is human.
15. A method for detecting an increased risk of a folate/cobalamin
metabolic disorder in a mammal, said method comprising detecting
the presence of a homozygous MTRR polymorphism that indicates an
increased risk of a folate/cobalamin metabolic disorder in said
mammal, wherein said polymorphism comprises (a) a G instead of an A
at position 66 relative to the first nucleotide of the start codon
of MTRR, (b) a deletion of 4 nucleotides starting from position
1675 (nucleotides 1675-1678) relative to the first nucleotide of
the start codon of MTRR, or (c) a deletion of 3 nucleotides
starting from nucleotide 1726 (nucleotides 1726-1728) relative to
the first nucleotide of the start codon of MTRR.
16. The method of claim 15, wherein said folate/cobalamin metabolic
disorder is megablastic anemia, developmental delay,
hyperhomocysteinuria, or hypomethionemia.
17. The method of claim 15, wherein said mammal is human.
18. The method of claim 15, further comprising measuring the level
of cobalamin in said mammal.
19. The method of claim 15, wherein said polymorphic MTRR is
detected by analyzing nucleic acid from said mammal.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. Ser. No.
09/487,841, filed Jan. 19, 2000, which claims priority from U.S.
Ser. No. 09/371,347, filed Aug. 10, 1999, which claims priority
from U.S. Ser. No. 09/232,028, filed on Jan. 15, 1999, which claims
priority from U.S. Provisional Application No. 60/071,622, filed
Jan. 16, 1998.
FIELD OF THE INVENTION
[0002] This invention relates to the diagnosis and treatment of
patients at risk for disorders associated with altered methionine
synthase activity.
BACKGROUND OF THE INVENTION
[0003] Methionine is an essential amino acid in mammals that is
required for protein synthesis. Methionine also plays a central
role in metabolic reactions involving transfer of single-carbon
moieties: in its activated form, S-adenosylmethionine, methionine
is the methyl donor in hundreds of biological transmethylation
reactions. Moreover, methionine is the propylamine donor in
polyamine synthesis. The ultimate product resulting from the
demethylation of methionine is homocysteine, the remethylation of
which is catalyzed by a cobalamin-dependent enzyme, methionine
synthase (5-methyltetrahydrofolate:homocysteine methyltransferase,
EC 2.1.1.13).
[0004] The enzyme-bound cobalamin cofactor of methionine synthase
plays an essential role in the methyl transfer reaction by acting
as an intermediate methyl carrier between methyltetrahydrofolate
and homocysteine. The upper portion of FIG. 1 illustrates the
transfer of the methyl group of methyltetrahydrofolate
(CH.sub.3-THF) to homocysteine via methionine
synthase-methylcobalamin [MetSyn-CH.sub.3--Co(III)] as an
intermediate methyl carrier. Cleavage of the methyl-cobalt bond of
the methylcob(III)alamin intermediate occurs heterolytically so as
to leave the cobalamin in the highly reactive cob(I)alamin
oxidation state. The occasional oxidation of the enzyme-cobalamin
to the cob(II)alamin state [MetSyn-Co(II)] renders the enzyme
inactive.
[0005] Severe deficiency of methionine synthase activity leads to
megaloblastic anemia, developmental delay, hyperhomocysteinemia,
and hypomethioninemia. Moreover, elevated plasma homocysteine is a
risk factor in cardiovascular disease and neural tube defects
(Rozen, Clin. Invest. Med. 19:171-178, 1996).
[0006] Two forms of methionine synthase deficiency are known
(Watkins et al., Am. J. Med. Genet. 34:427-434, 1989; Gulati et
al., J. Biol. Chem. 272:19171-19175, 1997). The first is a primary
defect of the amino acid sequence of the methionine synthase
enzyme. We recently cloned cDNAs encoding human methionine synthase
and showed that patients from the cblG complementation group of
folate/cobalamin metabolism have mutations in the methionine
synthase gene. A second class of patients, belonging to a distinct
complementation group, cblE, is also deficient in methionine
synthase enzymatic activity. The genetic basis of this deficiency
has not been determined.
[0007] An analogous methylcobalamin-dependent methionine synthase
has been well characterized in E. coli and the structures
comprising its C-terminal half have been elucidated by X-ray
crystallography. The reductive activation system required for its
maintenance is a two-component flavoprotein system consisting of
flavodoxin (a small FMN-containing electron transfer protein), and
NADPH-ferredoxin (flavodoxin) oxidoreductase, a member of a family
of electron transferases termed the "FNR family." However,
flavodoxins are not found in mammalian cells.
[0008] It would be desirable to identify the enzyme that catalyzes
the reductive activation of methionine synthase, i.e., the
methionine synthase reductase. Knowledge of the reductase wild-type
nucleotide and amino acid sequences would allow the identification
of mutations and polymorphisms associated with diseases involving
methionine metabolism. Moreover, an understanding of the reductase
structure and function will facilitate the identification of
compounds that modulate its activity. Such compounds will be useful
in treating and preventing disease and developmental defects.
BRIEF DESCRIPTION OF THE DRAWINGS
[0009] FIG. 1 is a diagram showing the enzymatic reaction that is
catalyzed by methionine synthase, and the reductive reactivation of
methionine synthase.
[0010] FIG. 2 is a diagram showing the overlapping clones and PCR
fragments used to clone and sequence human methionine synthase
reductase.
[0011] FIG. 3 is a diagram showing the nucleotide and deduced amino
acid sequence of human methionine synthase reductase.
[0012] FIG. 4 is a diagram showing an amino acid sequence
comparison among human methionine synthase reductase (HsMTRR; SEQ
ID NO: 21), C. elegans putative methionine synthase reductase
(CeMTRR; SEQ ID NO: 22) and human cytochrome P450 reductase (HsCPR;
SEQ ID NO: 23).
[0013] FIGS. 5A and 5B are representations of Northern blots
showing an analysis of methionine synthase reductase expression in
human tissues.
[0014] FIG. 6 is a diagram summarizing the FISH mapping of the
methionine synthase reductase gene to human chromosome
5p15.2-p15.3.
[0015] FIGS. 7A and 7B are representations of gels showing a
mutation analysis of cblE patient cell lines.
[0016] FIG. 7C is a diagram showing a sequence comparison of the
NADPH binding region of FNR family members (SEQ ID NOs: 25-40) FIG.
8A is a representation of two autoradiograms showing the A to G
polymorphism at MTRR coding position 66.
[0017] FIG. 8B is a representation of a gel showing a restriction
digest assay for distinguishing between the adenine 66 and guanine
66 alleles.
SUMMARY OF THE INVENTION
[0018] We have cloned the gene encoding human methionine synthase
reductase. This enzyme maintains methionine synthase in its
reduced, activated state, and hence is an essential component of
the methionine synthetic pathway. Deficiency of methionine synthase
reductase results in hyperhomocysteinemia, a condition that has
been implicated in cardiovascular disease and neural tube defects.
The presence of mutations in the methionine synthase reductase gene
that decrease methionine synthase reductase enzymatic activity are
likely to be associated with altered risk for cardiovascular
disease, neural tube defects, and cancer. The invention features
methods for risk detection and treatment of patients with
hyperhomocysteinemia, cardiovascular disease, neural tube defects,
and cancer. The invention also features compounds and kits which
may be used to practice the methods of the invention, methods and
compounds for treating or preventing these conditions and methods
of identifying therapeutics for the treatment or prevention of
these conditions.
[0019] In a first aspect, the invention features substantially pure
nucleic acid encoding a mammalian methionine synthase reductase
polypeptide. In various embodiments, the nucleic acid may encode a
human polypeptide, and the nucleic acid may be DNA, particularly
genomic DNA or cDNA. In another embodiment, the nucleic acid has
the sequence of SEQ ID NO: 1 or SEQ ID NO: 41, or degenerate
variants thereof, and the nucleic acid encodes the amino acid
sequence of SEQ ID NO: 2 or SEQ ID NO: 42. In yet another
embodiment, the nucleic acid is operably linked to regulatory
sequences for expression of methionine synthase reductase. The
regulatory sequences comprise a promoter, and the promoter may be
inducible.
[0020] In a second, related aspect, the invention features a
substantially pure nucleic acid that hybridizes at high stringency
to the nucleic acid of SEQ ID NO: 1 or SEQ ID NO: 41. In a
preferred embodiment, the nucleic acid is a naturally occurring
variant of the mammalian methionine synthase reductase gene. In
another embodiment, the nucleic acid has a sequence complementary
to at least 50% of at least 60 nucleotides of the nucleic acid
encoding the methionine synthase reductase polypeptide, and the
sequence is sufficient to allow nucleic acid hybridization under
high stringency conditions. In further embodiments, the nucleic
acid may be a probe or an antisense nucleic acid, and the sequence
may be complementary to at least 90% of at least 18 nucleotides of
the nucleic acid encoding the methionine synthase reductase
polypeptide.
[0021] In a third aspect, the invention features a nucleic acid
encoding a mutant or polymorphic mammalian methionine synthase
reductase polypeptide. In one embodiment, the nucleic acid may be
from a human. In another embodiment, the mutation is a deletion
mutation, for example, a deletion of 4 bases starting from base
1675 (bases 1675-1678) of SEQ ID NO:1 (SEQ ID NO: 47), or a
deletion of 3 bases starting from base 1726 (bases 1726-1728) of
SEQ ID NO:1 (SEQ ID NO: 45). In still another embodiment the
polymorphism is a nucleotide transition from G to A at nucleotide
position 66 (SEQ ID NO: 41), or from G to A at nucleotide position
110 (SEQ ID NO: 43). Other naturally-occurring variants associated
with altered risk for hyperhomocysteinemia are also a feature of
this aspect of the invention.
[0022] In a fourth, related aspect, the invention features a cell
containing the nucleic acid of the third aspect of the invention.
In various embodiments, the cell may be a prokaryotic cell, a
eukaryotic cell, a yeast cell, or a mammalian cell.
[0023] In a fifth, related aspect, the invention features a
non-human transgenic animal containing the nucleic acid of the
third aspect of the invention. In one embodiment, the nucleic acid
contains a mutation associated with hyperhomocysteinemia.
[0024] In a sixth, related aspect, the invention features a
non-human animal wherein one or both genetic alleles encoding a
methionine synthase reductase polypeptide are mutated. In one
embodiment of this sixth aspect, one or both genetic alleles
encoding a methionine synthase reductase polypeptide are disrupted,
deleted, or otherwise rendered nonfunctional. In further
embodiments of the fifth and sixth aspects, the animal may be a
rodent (e.g., a mouse), or a nematode (e.g., C. elegans).
[0025] In a seventh, related aspect, the invention features a cell
from the animal of the fifth and sixth aspects.
[0026] In an eighth aspect, the invention features a substantially
pure mammalian methionine synthase reductase polypeptide. In
various embodiments, the polypeptide may be recombinant, or may be
a human polypeptide, or may be the polypeptide set forth in SEQ ID
NO: 2 or SEQ ID NO: 42.
[0027] In a ninth, related aspect, the invention features a
polypeptide having conservative amino acid substitutions relative
to SEQ ID NO: 2 or SEQ ID NO: 42, and having methionine synthase
reductase biological activity.
[0028] In a tenth, related aspect, the invention features a mutant
or polymorphic polypeptide which has less methionine synthase
reductase biological activity than the polypeptide of SEQ ID NO: 2.
In preferred embodiments, the polypeptide has a frameshift
resulting in a premature stop codon (e.g., SEQ ID NO: 48), or a
deletion mutation, such as a deletion of Leu576 (SEQ ID NO: 46). In
other preferred embodiments, the polypeptide may have an amino acid
substitution, such as isoleucine instead of methionine at amino
acid position 22 (SEQ ID NO: 42), or tyrosine instead of cysteine
at amino acid position 37 (SEQ ID NO: 44).
[0029] In an eleventh, related aspect, the invention features a
mutant or polymorphic polypeptide which has higher methionine
synthase reductase biological activity than the polypeptide set
forth in SEQ ID NO: 2.
[0030] In a twelfth aspect, the invention features an antibody that
specifically binds a methionine synthase reductase polypeptide. In
one embodiment, the polypeptide is a mutant or polymorphic
polypeptide.
[0031] In a thirteenth, related aspect, the invention features a
method of generating an antibody that specifically binds a
methionine synthase reductase polypeptide. The method comprises
administering a methionine synthase reductase polypeptide, or
fragment thereof, to an animal capable of generating an immune
response, and isolating the antibody from the animal. Preferred
antibodies specifically bind mutant methionine synthase reductase
polypeptides.
[0032] In a fourteenth, related aspect, the invention features a
method of detecting the presence of a methionine synthase reductase
polypeptide. The method comprises contacting a sample with the
antibody that specifically binds a methionine synthase reductase
polypeptide and assaying for binding of the antibody to the
polypeptide.
[0033] In a fifteenth aspect, the invention features a method for
detecting sequence variants for methionine synthase reductase in a
mammal. The method comprises analyzing the nucleic acid of a test
subject to determine whether the test subject contains a mutation
or polymorphism in a methionine synthase reductase gene. The
presence of the mutation or polymorphism is an indication that the
animal has an increased or decreased likelihood of developing
hyperhomocysteinemia, cardiovascular disease, neural tube defects,
or cancer.
[0034] In one embodiment of the fifteenth aspect, primers used for
detecting a mutation are selected from: 5'-CTCCTGCTCGAACATCTTCCTAAA
(SEQ ID NO: 3); 5'-AATAGATAAT CCCTATCCTTATGCC (SEQ ID NO: 4);
5'-CCCTGGCTCCTAAGATATCCATC (SEQ ID NO: 5); 5'-CGAACAACAAA
TTCTTTCCACTTACC (SEQ ID NO: 6); 5'-CAAGGTTGGTGGAA GTCGCGTTG (SEQ ID
NO: 7); 5'-ATGCCTTGAAGTGAT GAGGAGGTTT (SEQ ID NO: 8);
5'-TTCCTACAACATAGAGAGAAACTC (SEQ ID NO: 9);
5'-TTGCACAAGGGCATCATGTACATC (SEQ ID NO: 10); 5'-AAACCTCC
TCATCACTTCAAGGCAT (SEQ ID NO: 11); 5'-CTTGCACACGAATATG GTCTGGG (SEQ
ID NO: 12); 5'-TGGCATCACCTGCATCCTTGAGG (SEQ ID NO: 13);
5'-GATGTACCTGTAAATATTCTGGGGG (SEQ ID NO: 14); 5'-AATCCACGGCTCAA
CCACAAGTTC (SEQ ID NO: 15); 5'-CTCGAAATT AACCCTCACTAAAGGG (SEQ ID
NO: 16); 5'-AACCCATACCGCAG GTGAGCAAA (SEQ ID NO: 17);
5'-TTTAGTACTTTCAGTCAAAA- AA GCTTAAT (SEQ ID NO: 18);
5'-ATAAACGACTTCAAGA GCTTGGAGC (SEQ ID NO: 19); or
5'-AGGTTTGGCACTAGTAAAGCTGACT (SEQ ID NO: 20).
[0035] In another embodiment of the fifteenth aspect of the
invention, the method further comprises the step of using nucleic
acid primers specific for the methionine synthase reductase gene.
The primers are used for DNA amplification by the polymerase chain
reaction. In yet another embodiment, the step further comprises the
step of sequencing nucleic acid encoding methionine synthase
reductase from the test subject. In still other embodiments, the
analyzing includes single strand conformational polymorphism (SSCP)
analysis, or the method is carried out by restriction fragment
length (RFLP) polymorphism analysis. In further embodiments, the
method is for the diagnosis of an altered risk for cardiovascular
disease, neural tube defects, or cancer, such as colon cancer.
[0036] In a sixteenth aspect, the invention features a kit for the
analysis of mammalian methionine synthase reductase nucleic acid.
The kit comprises nucleic acid probes for analyzing the nucleic
acid of a mammal, and the analyzing is sufficient to determine
whether the mammal contains a mutation in the methionine synthase
reductase nucleic acid. In a preferred embodiment the nucleic acid
probes allow detection of mutations associated with
hyperhomocysteinemia.
[0037] In a seventeenth aspect, the invention features a kit for
the analysis of mammalian methionine synthase reductase
polypeptides. The kit comprises antibodies for analyzing the
methionine synthase reductase polypeptide of a mammal, and the
analyzing is sufficient to determine whether the mammal contains a
mutation in the methionine synthase reductase nucleic acid.
[0038] In an eighteenth aspect, the invention features a method of
treating or preventing cancer, cardiovascular disease, or neural
tube defects. The method comprises inhibiting methionine synthase
reductase biological activity. In one embodiment, the mammal is
pregnant. In other embodiments, the method comprises administering
a therapeutically effective dose of a methionine synthase reductase
inhibitor to a mammal. The inhibitor may be a methionine synthase
reductase anti-sense nucleic acid, a peptide comprising a portion
of a mammalian methionine synthase reductase polypeptide, or a
small molecule.
[0039] In a nineteenth aspect, the invention features a method of
treating or preventing cardiovascular disease. The method comprises
administering to the subject a therapeutically effective dose of a
metabolite or cofactor selected from the group: folate, cobalamin,
S-adenosyl methionine, betaine, or methionine.
[0040] In a twentieth aspect, the invention features a method of
preventing neural tube defects, cancer, or cardiovascular disease.
The method comprises: a) detecting an increased risk of neural tube
defects, cancer, or cardiovascular disease, wherein the detecting
is performed by analyzing methionine synthase reductase nucleic
acid from one or more test subjects selected from: a mammal; a
potential parent, either male or female; a pregnant mammal; or a
developing embryo or fetus, wherein the analyzing is done by the
method of the fifteenth aspect of the invention; and b) exposing
the mammal, potential parent, pregnant mammal, and/or developing
embryo or fetus to a therapeutically effective dose of a metabolite
or cofactor selected from the group: cobalamin; S-adenosyl
methionine; betaine; or methionine, wherein the exposing is via the
administration of the dose to the mammal, the potential parent, the
pregnant mammal, and/or the developing embryo or fetus.
[0041] In a preferred embodiment of the eighteenth and twentieth
aspects of the invention, the subject has been diagnosed as having
a mutation or polymorphism in methionine synthase reductase.
[0042] In a twenty-first aspect, the invention features a method of
screening for a compound that modulates methionine synthase
reductase biological activity. The method comprises the steps of:
a) contacting a sample containing wild-type, mutated, or
polymorphic methionine synthase reductase with the compound, and b)
assaying for methionine synthase reductase enzymatic activity,
wherein increased enzymatic activity indicates an inducer of
methionine synthase reductase biological activity, and decreased
enzymatic activity indicates an inhibitor of methionine synthase
reductase biological activity.
[0043] In a twenty-second aspect, the invention features a method
for screening for a compound that modulates methionine synthase
reductase biological activity. The method comprises the steps of:
a) contacting a sample with the compound, and b) assaying for
methionine synthase reductase expression, wherein increased
expression indicates an inducer of methionine synthase reductase
biological activity, and decreased expression indicates an
inhibitor of methionine synthase reductase biological activity. The
sample is selected from: purified or partially purified methionine
synthase reductase, a cell lysate, a cell, a nematode, or a mammal.
In preferred embodiments, the sample may be the animal or cell
described by the fifth and sixth aspects of the invention. In other
preferred embodiments, the screening may be for compounds useful
for the treatment or prevention of cardiovascular disease or
cancer, or for the prevention of neural tube defects.
[0044] In a twenty-third aspect, the invention features a method
for detecting an increased risk of developing a neural tube defect
in a mammalian embryo or fetus. The method includes detecting the
presence of a polymorphic methionine synthase reductase (MTRR) in a
test subject, wherein the polymorphic MTRR contains a methionine
instead of an isoleucine at amino acid position 22, wherein the
test subject is a future parent of the embryo or fetus, and wherein
detection of a homozygous MTRR polymorphism in the future parent,
embryo, or fetus, or detection of either a homozygous or
heterozygous MTRR polymorphism in both future parents, indicates an
increased risk of developing a neural tube defect in the embryo or
fetus.
[0045] In various embodiments of the twenty-third aspect of the
invention, the polymorphic MTRR may be detected by analyzing
nucleic acid from the test subject. The nucleic acid may be genomic
DNA or cDNA. The nucleic acid may contain a G instead of an A at
the third position of the twenty-second codon (nucleotide position
66, relative to the first nucleotide of the start codon) of
MTRR.
[0046] In another embodiment of the twenty-third aspect of the
invention, the method may further include: a) PCR-amplifying a
segment of MTRR nucleic acid using primers MSG108S (SEQ ID NO: 49)
and AD292 (SEQ ID NO: 50), and b) digesting the product of the PCR
amplification reaction with the restriction enzyme Nde I, wherein a
PCR product that is digested by Nde I indicates an increased risk
of developing a neural tube defect in a mammalian embryo or
fetus.
[0047] In still other embodiments of the twenty-third aspect of the
invention, the polymorphic MTRR may be detected by analyzing MTRR
polypeptide from the test subject, and the test subject may be a
future female parent of the embryo or fetus, or the test subject
may be the embryo or fetus itself.
[0048] In yet further embodiments of the twenty-third aspect of the
invention, the method may further include detecting the presence of
a polymorphic methylenetetrahydrofolate reductase (MTHFR) in a test
subject, the polymorphic MTHFR having a T instead of a C at a
nucleotide position equivalent to position 677 of SEQ ID NO: 51,
wherein detection of the polymorphic MTHFR indicates an increased
risk of developing a neural tube defect in the embryo or fetus. The
polymorphic MTHFR may be detected by analyzing nucleic acid or
polypeptide from the test subject.
[0049] In still another embodiment of the twenty-third aspect of
the invention, the method may further include measuring the level
of cobalamin in the test subject, wherein a low cobalamin level
indicates an increased risk of developing a neural tube defect in
the embryo or fetus.
[0050] In a twenty-fourth aspect of the present invention, the
invention features a method for detecting an increased risk of
developing Down's Syndrome in a mammal, preferably a mammalian
embryo or fetus. In a twenty-fifth aspect the invention features a
method for detecting an increased risk of developing premature
coronary artery disease. Both aspects include detecting the
presence of a polymorphic MTRR in a test subject. Preferably, the
polymorphic MTRR contains a common A.fwdarw.G polymorphism at
position 66 of the MTRR cDNA sequence (SEQ ID NO: 1), wherein the
test subject is a mammal, preferably a future parent of an embryo
or fetus or an embryo or a fetus, and wherein detection of a
homozygous MTRR polymorphism indicates an increased risk of
developing a Down's Syndrome or coronary artery disease defect in
the embryo or fetus.
[0051] In various embodiments of the twenty-fourth aspect of the
invention, the polymorphic MTRR may be detected by analyzing
nucleic acid from the test subject. The nucleic acid may be genomic
DNA or cDNA. The nucleic acid may contain a G instead of an A at
the third position of the twenty-second codon (nucleotide position
66, relative to the first nucleotide of the start codon) of
MTRR.
[0052] In another embodiment of the twenty-fourth or twenty-fifth
aspects of the invention, the method may further include: a)
PCR-amplifying a segment of MTRR nucleic acid using primers MSG108S
(SEQ ID NO: 49) and AD292 (SEQ ID NO: 50) or A (SEQ ID NO:61) and B
(SEQ ID NO:62), and b) digesting the product of the PCR
amplification reaction with the restriction enzyme Nde I, wherein a
PCR product that is digested by Nde I indicates an increased risk
of developing a neural tube defect in a mammalian embryo or
fetus.
[0053] In still other embodiments of the twenty-fourth or
twenty-fifth aspects of the invention, the polymorphic MTRR may be
detected by analyzing MTRR polypeptide from the test subject, and
the test subject may be a future female parent of the embryo or
fetus, or the test subject may be the embryo or fetus itself.
[0054] In further aspects of the invention, the invention features
a method for detecting the presence of a polymorphic
methylenetetrahydrofolate reductase (MTHFR) in a test subject,
(preferably an MTHFR having a T instead of a C at a nucleotide
position equivalent to position 677 of SEQ ID NO: 51), wherein
detection of the polymorphic MTHFR indicates an increased risk of
developing Down's Syndrome in the embryo or fetus. The polymorphic
MTHFR may be detected by analyzing nucleic acid or polypeptide from
the test subject.
[0055] In still another embodiment of the twenty-fourth or
twenty-fifth aspects of the invention, the method may further
include measuring the level of cobalamin in the test subject,
wherein a low cobalamin level indicates an increased risk of
developing Down's Syndrome or premature cardiovascular disease in
the embryo or fetus.
[0056] By "methionine synthase reductase," "methionine synthase
reductase protein," or "methionine synthase reductase polypeptide"
is meant a polypeptide, or fragment thereof, which has at least 43%
amino acid sequence identity, or at least 53% sequence similarity,
preferably at least 47% identity (or at least 57% similarity), more
preferably at least 55% identity (or at least 65% similarity), yet
more preferably at least 65% sequence identity (or at least 75%
similarity), still more preferably at least 75% sequence identity
(or at least 85% similarity) and most preferably at least 85%
sequence identity (or at least 95% similarity) to the human
methionine synthase reductase polypeptide of SEQ ID NO: 2 (see FIG.
4), over the length of the polypeptide or fragment thereof, or over
the length of the human methionine synthase reductase polypeptide
of SEQ ID NO: 2, whichever is shorter in length. It is understood
that polypeptide products from splice variants of methionine
synthase reductase gene sequences are also included in this
definition. Preferably, the methionine synthase reductase protein
is encoded by nucleic acid having a sequence which hybridizes to a
nucleic acid sequence present in SEQ ID NO: 1 (human methionine
synthase reductase cDNA) under stringent conditions. Even more
preferably the encoded polypeptide also has methionine synthase
reductase biological activity, or is a mutant or polymorphic form
of methionine synthase reductase that is associated with an
increased risk of disease.
[0057] By "methionine synthase reductase nucleic acid" or
"methionine synthase reductase gene" is meant a nucleic acid, such
as genomic DNA, cDNA, or mRNA, that encodes methionine synthase
reductase, a methionine synthase reductase protein, methionine
synthase reductase polypeptide, or portion thereof, as defined
above.
[0058] By "mutant methionine synthase reductase," "methionine
synthase reductase mutation(s)," "mutations in methionine synthase
reductase," "polymorphic methionine synthase reductase,"
"methionine synthase reductase polymorphism(s)," "polymorphisms in
methionine synthase reductase," is meant a methionine synthase
reductase (MTTR) polypeptide or nucleic acid having a sequence that
confers an increased risk of a disease phenotype or enhanced
protection against a disease in at least some genetic and/or
environmental backgrounds. An example of a disease-associated
methionine synthase reductase polymorphism is the 22M polymorphism
(SEQ ID NO: 2), which is associated with an increased risk for
neural tube defects.
[0059] Any given methionine synthase reductase polymorphism may be
associated with an increased risk for some diseases and a decreased
risk for other dieseases. Increased or decreased disease risks
associated with specific methionine synthase reductase mutations
and polymorphisms are determined by methods known to those skilled
in the art.
[0060] Such mutations may be naturally occurring, or artificially
induced. They may be, without limitation, transition, transversion,
insertion, deletion, frameshift, or missense mutations. A mutant
methionine synthase reductase protein may have one or more
mutations, and such mutations may affect different aspects of
methionine synthase reductase biological activity (protein
function), to various degrees. Alternatively, a methionine synthase
reductase mutation may indirectly affect methionine synthase
reductase biological activity by influencing, for example, the
transcriptional activity of a gene encoding methionine synthase
reductase, or the stability of methionine synthase reductase mRNA.
For example, a mutant methionine synthase reductase gene may be a
gene that expresses a mutant methionine synthase reductase protein
or may be a gene which alters the level of methionine synthase
reductase protein in a manner sufficient to confer a disease
phenotype in at least some genetic and/or environmental
backgrounds. The presence of polymorphic or mutant methionine
synthase reductase may be determined by detecting polymorphic or
mutant methionine synthase reductase nucleic acid or polypeptide,
using methods that are known in the art.
[0061] By "biologically active" methionine synthase reductase is
meant a methionine synthase reductase protein or methionine
synthase reductase gene that provides at least one biological
function equivalent to that of the wild-type methionine synthase
reductase polypeptide or the methionine synthase reductase gene.
Biological activity of a methionine synthase reductase polypeptide
includes, but is not limited to, the ability to catalyze the
reductive methylation of enzymatically inactive methionine
synthase-cob(II)alamin to generate enzymatically active methionine
synthase-cob(III)alamin-CH3. Preferably, a biologically active
methionine synthase reductase will display activity equivalent to
at least 20-30% of wild-type activity, more preferably, at least
35-50% of wild-type activity, still more preferably, 55-75% of
wild-type activity, and most preferably, a biologically active
methionine synthase reductase will display at least 80-90% of
wild-type activity. A biologically active methionine synthase
reductase also may display more than 100% of wild-type activity.
Preferably, the biological activity of the wild-type methionine
synthase reductase is determined using the methionine synthase
reductase nucleic acid of SEQ ID NO: 1 or SEQ ID NO: 41 or
methionine synthase reductase polypeptide of SEQ ID NO: 2 or SEQ ID
NO: 42. The degree of methionine synthase reductase biological
activity may be intrinsic to the methionine synthase reductase
polypeptide itself, or may be modulated by increasing or decreasing
the number of methionine synthase reductase polypeptide molecules
present intracellularly.
[0062] By "high stringency conditions" is meant hybridization in
2.times.SSC at 40.degree. C. with a DNA probe length of at least 40
nucleotides. For other definitions of high stringency conditions,
see Ausubel et al., Current Protocols in Molecular Biology, pp.
6.3.1-6.3.6, John Wiley & Sons, New York, N.Y., 1998, hereby
incorporated by reference.
[0063] By "analyzing" or "analysis" is meant subjecting a
methionine synthase reductase nucleic acid or methionine synthase
reductase polypeptide to a test procedure that allows the
determination of whether a methionine synthase reductase gene is
wild-type or mutant. For example, one could analyze the methionine
synthase reductase genes of an animal by amplifying genomic DNA
using the polymerase chain reaction, and then determining the DNA
sequence of the amplified DNA.
[0064] By "probe" or "primer" is meant a single-stranded DNA or RNA
molecule of defined sequence that can base pair to a second DNA or
RNA molecule that contains a complementary sequence (the "target").
The stability of the resulting hybrid depends upon the extent of
the base pairing that occurs. The extent of base-pairing is
affected by parameters such as the degree of complementarity
between the probe and target molecules, and the degree of
stringency of the hybridization conditions. The degree of
hybridization stringency is affected by parameters such as
temperature, salt concentration, and the concentration of organic
molecules such as formamide, and is determined by methods known to
one skilled in the art. Probes or primers specific for methionine
synthase reductase nucleic acid preferably will have at least 35%
sequence identity, more preferably at least 45-55% sequence
identity, still more preferably at least 60-75% sequence identity,
still more preferably at least 80-90% sequence identity, and most
preferably 100% sequence identity. Probes may be
detectably-labelled, either radioactively, or non-radioactively, by
methods well-known to those skilled in the art. Probes are used for
methods involving nucleic acid hybridization, such as: nucleic acid
sequencing, nucleic acid amplification by the polymerase chain
reaction, single stranded conformational polymorphism (SSCP)
analysis, restriction fragment polymorphism (RFLP) analysis,
Southern hybridization, Northern hybridization, in situ
hybridization, electrophoretic mobility shift assay (EMSA).
[0065] By "pharmaceutically acceptable carrier" means a carrier
which is physiologically acceptable to the treated mammal while
retaining the therapeutic properties of the compound with which it
is administered. One exemplary pharmaceutically acceptable carrier
is physiological saline. Other physiologically acceptable carriers
and their formulations are known to one skilled in the art and
described, for example, in Remington's Pharmaceutical Sciences,
(18.sup.th edition), ed. A. Gennaro, 1990, Mack Publishing Company,
Easton, Pa.
[0066] By "substantially identical" is meant a polypeptide or
nucleic acid exhibiting, over its entire length, at least 50%,
preferably 85%, more preferably 90%, and most preferably 95%
identity to a reference amino acid or nucleic acid sequence. For
polypeptides, the length of comparison sequences will generally be
at least 16 amino acids, preferably at least 20 amino acids, more
preferably at least 25 amino acids, and most preferably 35 amino
acids. For nucleic acids, the length of comparison sequences will
generally be at least 50 nucleotides, preferably at least 60
nucleotides, more preferably at least 75 nucleotides, and most
preferably 110 nucleotides.
[0067] By "identity" is meant that a polypeptide or nucleic acid
sequence possesses the same amino acid or nucleotide residue at a
given position, compared to a reference polypeptide or nucleic acid
sequence to which the first sequence is aligned.
[0068] Sequence identity is typically measured using sequence
analysis software with the default parameters specified therein
(e.g., Sequence Analysis Software Package of the Genetics Computer
Group, University of Wisconsin Biotechnology Center, 1710
University Avenue, Madison, Wis. 53705). This software program
matches similar sequences by assigning degrees of homology to
various substitutions, deletions, and other modifications.
Conservative substitutions typically include substitutions within
the following groups: glycine, alanine, valine, isoleucine,
leucine; aspartic acid, glutamic acid; asparagine, glutamine;
serine, threonine; lysine, arginine; and phenylalanine,
tyrosine.
[0069] By "substantially pure polypeptide" is meant a polypeptide
that has been separated from the components that naturally
accompany it. Typically, the polypeptide is substantially pure when
it is at least 60%, by weight, free from the proteins and
naturally-occurring organic molecules with which it is naturally
associated. Preferably, the polypeptide is a methionine synthase
reductase polypeptide that is at least 75%, more preferably at
least 90%, and most preferably at least 99%, by weight, pure. A
substantially pure methionine synthase reductase polypeptide may be
obtained, for example, by extraction from a natural source (e.g., a
fibroblast) by expression of a recombinant nucleic acid encoding a
methionine synthase reductase polypeptide, or by chemically
synthesizing the protein. Purity can be measured by any appropriate
method, e.g., by column chromatography, polyacrylamide gel
electrophoresis, or HPLC analysis.
[0070] A protein is substantially free of naturally associated
components when it is separated from those contaminants which
accompany it in its natural state. Thus, a protein which is
chemically synthesized or produced in a cellular system different
from the cell from which it naturally originates will be
substantially free from its naturally associated components.
Accordingly, substantially pure polypeptides not only includes
those derived from eukaryotic organisms but also those synthesized
in E. coli or other prokaryotes.
[0071] By "substantially pure DNA" is meant DNA that is free of the
genes which, in the naturally-occurring genome of the organism from
which the DNA of the invention is derived, flank the gene. The term
therefore includes, for example, a recombinant DNA which is
incorporated into a vector; into an autonomously replicating
plasmid or virus; or into the genomic DNA of a prokaryote or
eukaryote; or which exists as a separate molecule (e.g., a cDNA or
a genomic or cDNA fragment produced by PCR or restriction
endonuclease digestion) independent of other sequences. It also
includes a recombinant DNA which is part of a hybrid gene encoding
additional polypeptide sequence.
[0072] By "transgene" is meant any piece of DNA that is inserted by
artifice into a cell, and becomes part of the genome of the
organism that develops from that cell. Preferably the coding region
of the transgene is operably linked to one or more transcriptional
regulatory elements, including a promoter (as defined below) that
direct transgene expression. Such a transgene may comprise a gene
which is partly or entirely heterologous (i.e., foreign) to the
transgenic organism, or may represent a gene homologous to an
endogenous gene of the organism.
[0073] By "transgenic" is meant any cell that includes a DNA
sequence that is inserted by artifice into a cell and becomes part
of the genome of the organism which develops from that cell. As
used herein, the transgenic organisms are generally transgenic
mammals (e.g., rodents such as rats or mice) and the DNA
(transgene) is inserted by artifice into the genome. Transgenic
organisms also may include transgenic nematodes, such as transgenic
Caenorrhabditis elegans, which are generated by methods known to
those skilled in the art.
[0074] By "knockout mutation" is meant an alteration in the nucleic
acid sequence that reduces the biological activity of the
polypeptide normally encoded therefrom by at least 80% relative to
the unmutated gene. The mutation may, without limitation, be an
insertion, deletion, frameshift mutation, or a missense mutation.
Preferably, the mutation is an insertion or deletion, or is a
frameshift mutation that creates a stop codon.
[0075] By "transformation" is meant any method for introducing
foreign molecules into a cell (e.g., a bacterial, yeast, fungal,
algal, plant, insect, or animal cell). Lipofection,
DEAE-dextran-mediated transfection, microinjection, protoplast
fusion, calcium phosphate precipitation, retroviral delivery,
electroporation, and biolistic transformation are just a few of the
methods known to those skilled in the art which may be used.
[0076] By "transformed cell" is meant a cell (or a descendant of a
cell) into which a DNA molecule encoding a methionine synthase
reductase polypeptide has been introduced, by means of recombinant
DNA techniques.
[0077] By "positioned for expression" is meant that the DNA
molecule is positioned adjacent to a DNA sequence which directs
transcription and translation of the sequence (i.e., facilitates
the production of, e.g., a methionine synthase reductase
polypeptide, a recombinant protein or a RNA molecule).
[0078] By "promoter" is meant a minimal sequence sufficient to
direct transcription. Also included in the invention are those
promoter elements which are sufficient to render promoter-dependent
gene expression controllable for cell type-specific,
tissue-specific, temporal-specific, or inducible by external
signals or agents; such elements may be located in the 5' or 3' or
intron sequence regions of the native gene.
[0079] By "operably linked" is meant that a gene and one or more
regulatory sequences are connected in such a way as to permit gene
expression when the appropriate molecules (e.g., transcriptional
activator proteins) are bound to the regulatory sequences.
[0080] By "conserved region" is meant any stretch of six or more
contiguous amino acids exhibiting at least 30%, preferably at least
50%, and most preferably at least 70% amino acid sequence identity
between two or more reductase family members, (e.g., between human
methionine synthase reductase and human cytochrome p450 reductase).
An example of a conserved region within these two reductases is the
NADPH binding region (FIG. 4).
[0081] By "detectably-labeled" is meant any means for marking and
identifying the presence of a molecule, e.g., an oligonucleotide
probe or primer, a gene or fragment thereof, or a cDNA molecule.
Methods for detectably-labeling a molecule are well known in the
art and include, without limitation, radioactive labeling (e.g.,
with an isotope such as .sup.32P or .sup.35S) and nonradioactive
labeling (e.g., chemiluminescent or fluorescent labeling, e.g.,
fluorescein labeling).
[0082] By "antisense" as used herein in reference to nucleic acids,
is meant a nucleic acid sequence that is complementary to the
coding strand of a gene, preferably, a methionine synthase
reductase gene. An antisense nucleic acid is capable of
preferentially decreasing the activity of a mutant methionine
synthase reductase polypeptide encoded by a mutant methionine
synthase reductase gene.
[0083] By "specifically binds" is meant that an antibody recognizes
and binds a human methionine synthase reductase polypeptide, but
does not substantially recognize and bind other non-methionine
synthase reductase molecules in a sample, e.g., a biological
sample, that naturally includes protein. A preferred antibody binds
to the methionine synthase reductase polypeptide sequence of SEQ ID
NO: 2 (FIG. 3).
[0084] By "neutralizing antibodies" is meant antibodies that
interfere with any of the biological activities of a wild-type or
mutant methionine synthase reductase polypeptide, for example, the
ability of methionine synthase reductase to catalyze the transfer
of a methyl group to methionine synthase-cobal(II)amin. The
neutralizing antibody may reduce the ability of a methionine
synthase reductase polypeptide to catalyze the transfer preferably
by 10% or more, more preferably by 25% or more, still more
preferably by 50% or more, yet preferably by 70% or more, and most
preferably by 90% or more. Any standard assay for the biological
activity of methionine synthase reductase may be used to assess
potentially neutralizing antibodies that are specific for
methionine synthase reductase.
[0085] By "expose" is meant to allow contact between an animal,
cell, lysate or extract derived from a cell, or molecule derived
from a cell, and a test compound.
[0086] By "treat" is meant to submit or subject an animal (e.g. a
human), cell, lysate or extract derived from a cell, or molecule
derived from a cell to a test compound.
[0087] By "test compound" is meant a chemical, be it
naturally-occurring or artificially-derived, that is surveyed for
its ability to modulate an alteration in reporter gene activity or
protein levels, by employing one of the assay methods described
herein. Test compounds may include, for example, peptides,
polypeptides, synthesized organic molecules, naturally occurring
organic molecules, nucleic acid molecules, and components
thereof.
[0088] By "assaying" is meant analyzing the effect of a treatment,
be it chemical or physical, administered to whole animals, cells,
or lysates, extracts, or molecules derived therefrom. The material
being analyzed may be an animal, a cell, a lysate or extract
derived from a cell, or a molecule derived from a cell. The
analysis may be for the purpose of detecting altered protein
biological activity, altered protein stability, altered protein
levels, altered gene expression, or altered RNA stability. The
means for analyzing may include, for example, the detection of the
product of an enzymatic reaction, (e.g., the formation of active
methionine synthase or methionine as a result of methionine
synthase reductase activity), antibody labeling,
immunoprecipitation, and methods known to those skilled in the art
for detecting nucleic acids.
[0089] By "modulating" is meant changing, either by decrease or
increase, in biological activity.
[0090] By "a decrease" is meant a lowering in the level of
biological activity, as measured by inhibition of: a) the formation
of enzymatically active methionine synthase-cob(III)alamin-CH3 or
methionine as a result of methionine synthase reductase activity;
b) protein, as measured by ELISA; c) reporter gene activity, as
measured by reporter gene assay, for example,
lacZ/.beta.-galactosidase, green fluorescent protein, luciferase,
etc.; or d) mRNA, as measured by PCR relative to an internal
control, for example, a "housekeeping" gene product such as
.beta.-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
In all cases, the decrease is preferably by at least 10% more
preferably by at least 25%, still more preferably by at least 50%,
and even more preferably by at least 70%.
[0091] By "an increase" is meant a rise in the level of biological
activity, as measured by a stimulation of: a) the formation of
methionine synthase-cob(III)alamin-CH3 or methionine as a result of
methionine synthase reductase activity; b) protein, as measured by
ELISA; c) reporter gene activity, as measured by reporter gene
assay, for example, lacZ/.beta.-galactosidase, green fluorescent
protein, luciferase, etc.; or d) mRNA, as measured by PCR relative
to an internal control, for example, a "housekeeping" gene product
such as .beta.-actin or glyceraldehyde 3-phosphate dehydrogenase
(GAPDH). Preferably, the increase is by at least 10%, more
preferably by at least 25%, still more preferably by at least 75%,
even more preferably by 2-fold, and most preferably by at least
3-fold.
[0092] By "alteration in the level of gene expression" is meant a
change in gene activity such that the amount of a product of the
gene, i.e., mRNA or polypeptide, is increased or decreased, or that
the stability of the mRNA or the polypeptide is increased or
decreased.
[0093] By "reporter gene" is meant any gene that encodes a product
whose expression is detectable and/or quantitatable by
immunological, chemical, biochemical or biological assays. A
reporter gene product may, for example, have one of the following
attributes, without restriction: fluorescence (e.g., green
fluorescent protein), enzymatic activity (e.g.,
lacZ/.beta.-galactosidase, luciferase, chloramphenicol
acetyltransferase), toxicity (e.g., ricin A), or an ability to be
specifically bound by a second molecule (e.g., biotin or a
detectably-labelled antibody). It is understood that any engineered
variants of reporter genes, which are readily available to one
skilled in the art, are also included, without restriction, in the
forgoing definition.
[0094] By "protein" or "polypeptide" or "polypeptide fragment" is
meant any chain of more than two amino acids, regardless of
post-translational modification (e.g., glycosylation or
phosphorylation), constituting all or part of a naturally-occurring
polypeptide or peptide, or constituting a non-naturally occurring
polypeptide or peptide.
[0095] By "missense mutation" is meant the substitution of one
purine or pyrimidine base (i.e. A, T, G, or C) by another within a
nucleic acid sequence, such that the resulting new codon may encode
an amino acid distinct from the amino acid originally encoded by
the reference (e.g. wild-type) codon.
[0096] By "deletion mutation" is meant the deletion of at least one
nucleotide within a polynucleotide coding sequence. A deletion
mutation alters the reading frame of a coding region unless the
deletion consists of one or more contiguous 3-nucleotide stretches
(i.e. "codons"). Deletion of a codon from a nucleotide coding
region results in the deletion of an amino acid from the resulting
polypeptide.
[0097] By "frameshift mutation" is meant the insertion or deletion
of at least one nucleotide within a polynucleotide coding sequence.
A frameshift mutation alters the codon reading frame at and/or
downstream from the mutation site. Such a mutation results either
in the substitution of the encoded wild-type amino acid sequence by
a novel amino acid sequence, or a premature termination of the
encoded polypeptide due to the creation of a stop codon, or
both.
[0098] By "low serum cobalamin level" is meant a serum cobalamin
concentration of less than 328 pmol/L in a child, fetus, or embryo
that has a neural tube defect or is at risk for developing a neural
tube defect, or a serum cobalamin concentration of less than 259
pmol/L in the mother or future parent of a child having a neural
tube defect.
[0099] By "polymorphic methylenetetrahydrofolate reductase" or
"mutant methylenetetrahydrofolate reductase" is meant
methylenetetrahydrofolate reductase (MTHFR) polypeptide or nucleic
acid having a sequence that confers an increased risk of a disease
phenotype in at least some genetic and/or environmental
backgrounds, for example, in combination with an MMTR polymorphism
or mutation.
[0100] By "677C.fwdarw.T polymorphism in MTHFR" is meant a
substitution of cytosine in place of thymine in nucleic acid
encoding MTHFR at a nucleotide position equivalent to MTHFR
nucleotide position 677 as disclosed in Frosst et al. (Nat. Genet.
10:111-113, 1995) and in Genbank Accession No. U09806 (SEQ ID NO:
51).
[0101] By "future parent" is meant a male or female who has
contributed or may potentially contribute genetic material (e.g., a
sperm or an egg) to form a zygote. A future parent is also a female
who gestates or may potentially gestate an embryo or fetus in her
uterus, irrespective of whether she has contributed or may
potentially contribute genetic material to the embryo or fetus; an
example of such a future parent is a surrogate mother).
[0102] By "test subject" is meant a future parent as defined above,
an embryo, or a fetus.
[0103] By "sample from a test subject" is meant a specimen, for
example, and not limited to, blood, serum, cells, or amniotic
fluid, that would allow one of skill in the art to determine
whether the test subject has a mutant or polymorphic methionine
synthase reductase.
[0104] By "cardiovascular disease" is meant cardiovascular disease
associated with elevated plasma homocysteine as described in
(Rozen, Clin. Invest. Med. 19:171-178, 1996). As used herein, the
term cardiovascular disease includes premature coronary artery
disease.
DETAILED DESCRIPTION OF THE INVENTION
[0105] Methionine synthase catalyzes the remethylation of
homocysteine to methionine in a reaction in which methylcobalamin
serves as an intermediate methyl carrier.
[0106] Over time, the cob(I)alamin cofactor of methionine synthase
may become oxidized to cob(II)alamin, thus rendering the enzyme
inactive. Regeneration of the functional enzyme occurs through the
reductive methylation of the cob(II)alamin in a reaction in which
S-adenosylmethionine is utilized as methyl donor (FIG. 1). The
reductive activation system in the lower part of the scheme shown
in FIG. 1 is the mechanism by which S-adenosylmethionine (Ado-Met)
together with an electron reactivates the enzyme to the functional,
methionine synthase-CH3-Co(III) state, resulting in the formation
of S-adenosylhomocysteine (Ado-Hcy) as a reaction by-product.
[0107] Patients of the cblE complementation group of disorders of
folate/cobalamin metabolism, who are defective in the reductive
activation of methionine synthase, have megaloblastic anemia,
developmental delay, hyperhomocysteinemia, and hypomethioninemia.
We have cloned a cDNA corresponding to the "methionine synthase
reductase" reducing system required for maintenance of the
methionine synthase in a functional state. Using primers comprising
sequences of consensus binding sites for FAD, FMN and NADPH, we
performed RT-PCR and inverse PCR to clone a methionine synthase
reductase cDNA. The cDNA hybridizes to an mRNA of 3.6 kb (as
detected by Northern blot). The deduced protein is a novel member
of the FNR family of electron transferases, containing 698 amino
acids with a predicted Mr of 77,700. It shares 38% identity with
human cytochrome P450 reductase and 43% with the C. elegans
putative methionine synthase reductase (see below). Methionine
synthase reductase was localized to human chromosome 5p 15.2-15.3
by fluorescence in situ hybridization (FISH).
[0108] A survey of the NCBI databases for homology to the human
methionine synthase reductase using BLASTP or TBLASTN yielded the
putative methionine synthase reductase of C. elegans (P
value=9.times.10-92). Proteins of the FNR family were also found
using the BLAST programs. The strongest homology was found with
cytochrome P450 reductase (P values>3.times.10-68), followed by
nitric oxide synthase (three isoforms, P values>4.times.10-52),
and sulfite reductase (P values>6.times.10-39). Lower, but still
significant homology was found with E. coli NADPH-ferredoxin
(flavodoxin) reductase (P values>2.times.10-9) and flavodoxin (P
values>3.times.10-2). Our finding suggests a convergent
evolution of the two-gene flavodoxin/NADPH-ferredoxin (flavodoxin)
reductase system to a single gene encoding a fused version of the
two proteins in human cells. Alignment of the proteins provides for
a large linker region bridging the two components.
[0109] The identity of our cloned cDNA sequence as that encoding
methionine synthase reductase was confirmed by the identification
of mutations in the corresponding gene in cblE patients having a
functional deficiency of methionine synthase. Our key finding
confirming the identification of the cDNA was a 4 bp frameshift
mutation in two affected siblings. The occurrence of a functionally
null mutation in a candidate gene provides compelling evidence that
the mutation is causative of disease in the affected patients.
Furthermore, a 3 bp deletion detected in a third patient is also
highly likely to cause an enzyme defect, and the direct sequencing
of PCR products suggested that the patient's second allele contains
a mutation that renders the mRNA very unstable or poorly
transcribed. In all, seven of ten tested cblE cell lines showed
evidence of mutation although the sequence changes have yet to be
determined in the remaining four.
[0110] The two mutations we have identified associated with cblE
disease are located in the vicinity of the NADPH binding domain by
comparison with proteins of the FNR family. The 4 bp deletion
yields a truncated protein that is expected to be deficient in
NADPH binding and possibly in FAD binding, since the C-terminus of
the enzyme may be involved in both. The 3 bp deletion results in
the deletion of Leu576, which is located between two sequences that
may be involved in NADPH binding. Leu576 is well conserved among
reductases that are similar to the methionine synthase reductase
(FIG. 6C). This supports the idea that deletion of the Leu576 codon
(1726delTTG) results in an enzymatic defect, although confirmation
will require expression of the mutant protein. This residue is also
conserved in the NADPH-ferredoxin (flavodoxin) reductase enzymes of
several organisms, although the homology with this portion of the
protein is low or absent in some cases. It is possible that the
deletion affects the relationship between the two NADPH-binding
sequences that are in its vicinity.
[0111] The cloning of human methionine synthase reductase cDNA
enables the determination of the enzymatic mechanism involved in
the reductive activation of methionine synthase. Furthermore, it is
now possible to identify additional mutations in patients with
severe deficiency of the enzyme activity, and to determine whether
there exist common amino acid polymorphisms which lead to mildly
elevated homocysteine levels. Such elevations may be a risk factor
in cardiovascular disease, neural tube defects, and cancer.
[0112] Mutations in the human methionine synthase reductase gene
that result in altered homocysteine and/or folate levels may be
risk factors for the diseases listed above. The methods of the
invention therefore provide diagnostic assays for such risk
factors, as well as methods of treating or preventing
cardiovascular disease, neural defects, cancer, megaloblastic
anemia, and hypomethioninemia. In addition, the invention provides
methods for screening assays for the isolation of potential
therapeutic compounds that modulate methionine synthase reductase
activity.
[0113] The assays described herein can be used to test for
compounds that modulate methionine synthase activity and hence may
have therapeutic value in the prevention of neural tube defects,
prevention and/or treatment of cancer, cardiovascular disease,
homocysteinemia, and megaloblastic anemia.
[0114] Test Compounds
[0115] In general, novel drugs for prevention of neural tube
defects, or prevention and/or treatment of cancer, cardiovascular
disease, and megaloblastic anemia are identified from large
libraries of both natural product or synthetic (or semi-synthetic)
extracts or chemical libraries according to methods known in the
art. Those skilled in the field of drug discovery and development
will understand that the precise source of test extracts or
compounds is not critical to the screening procedure(s) of the
invention. Accordingly, virtually any number of chemical extracts
or compounds can be screened using the exemplary methods described
herein. Examples of such extracts or compounds include, but are not
limited to, plant-, fungal-, prokaryotic- or animal-based extracts,
fermentation broths, and synthetic compounds, as well as
modification of existing compounds. Numerous methods are also
available for generating random or directed synthesis (e.g.,
semi-synthesis or total synthesis) of any number of chemical
compounds, including, but not limited to, saccharide-, lipid-,
peptide-, and nucleic acid-based compounds. Synthetic compound
libraries are commercially available from Brandon Associates
(Merrimack, N.H.) and Aldrich Chemical (Milwaukee, Wis.).
Alternatively, libraries of natural compounds in the form of
bacterial, fungal, plant, and animal extracts are commercially
available from a number of sources, including Biotics (Sussex, UK),
Xenova (Slough, UK), Harbor Branch Oceangraphics Institute (Ft.
Pierce, Fla.), and PharmaMar, U.S.A. (Cambridge, Mass.). In
addition, natural and synthetically produced libraries are
produced, if desired, according to methods known in the art, e.g.,
by standard extraction and fractionation methods. Furthermore, if
desired, any library or compound is readily modified using standard
chemical, physical, or biochemical methods.
[0116] In addition, those skilled in the art of drug discovery and
development readily understand that methods for dereplication
(e.g., taxonomic dereplication, biological dereplication, and
chemical dereplication, or any combination thereof) or the
elimination of replicates or repeats of materials already known for
their therapeutic activities for homocysteinemia, megaloblastic
anemia, cardiovascular disease, cancer, and neural tube defects
should be employed whenever possible.
[0117] When a crude extract is found to modulate methionine
synthase reductase biological activity, further fractionation of
the positive lead extract is necessary to isolate chemical
constituents responsible for the observed effect. Thus, the goal of
the extraction, fractionation, and purification process is the
careful characterization and identification of a chemical entity
within the crude extract that modulates methionine synthase
reductase biological activity. The same assays described herein for
the detection of activities in mixtures of compounds can be used to
purify the active component and to test derivatives thereof.
Methods of fractionation and purification of such heterogenous
extracts are known in the art. If desired, compounds shown to be
useful agents for treatment are chemically modified according to
methods known in the art. Compounds identified as being of
therapeutic value may be subsequently analyzed using mammalian
models of homocysteinemia, megaloblastic anemia, cardiovascular
disease, cancer, and neural tube defects.
[0118] Methionine Synthase Reductase Assays for the Detection of
Compounds that Modulate Methionine Synthase Reductase Activity and
Expression
[0119] Potentially useful therapeutic compounds that modulate (e.g.
increase or decrease) methionine synthase reductase activity or
expression may be isolated by various screens that are well-known
to those skilled in the art. Such compounds may modulate methionine
synthase reductase expression at the pre- or post-transcriptional
level, or at the pre- or post-translational level.
[0120] A. Screens for Compounds that Modulate Methionine Synthase
Reductase Enzymatic Activity
[0121] Screens for potentially useful therapeutic compounds that
modulate methionine synthase reductase activity may be readily
performed. For example, the effect of a test compound on methionine
synthase reductase activity may be determined by measuring
formation of .sup.14CH.sub.3-cob(III)alamin, which results from the
transfer of .sup.14CH.sub.3 from S-adenosylmethionine to methionine
synthase-cob(II)alamin. A test compound that increases the
enzymatic activity of a methionine synthase reductase would result
in increased levels of methionine
synthase-.sup.14CH.sub.3-cob(III)alamin, and a compound that
decreases the enzymatic activity of a methionine synthase reductase
would result in decreased levels of methionine
synthase-.sup.14CH.sub.3-cob(III)alamin.
[0122] The effect of a test compound on methionine synthase
reductase activity also may be determined by measuring the
resulting activity of methionine synthase. The amount of reaction
product (i.e., methionine) formation reflects the relative activity
of methionine synthase, which in turn reflects the relative
activity of methionine synthase reductase, which in turn indicates
the effect of the test compound on methionine synthase reductase
activity. For example, a sample containing methionine synthase and
homocysteine may contain a mutant, inactive methionine synthase
reductase which does not reduce oxidized methionine synthase, and
hence, no methionine is formed. However, a test compound that
increases the enzymatic activity of the mutant methionine synthase
reductase will result in increased levels of methionine formation,
relative to control samples not containing the test compound.
Analogously, a compound that decreases methionine synthase
reductase activity will result in the formation of decreased levels
of methionine formation in reactions containing active methionine
synthase reductase. That a test compound directly modulates
methionine synthase reductase enzymatic activity, as opposed to
methionine synthase enzymatic activity, can be confirmed by
including control reactions that lack methionine synthase
reductase. Such control reactions should not show altered levels of
methionine production if the test compound directly modulates
methionine synthase reductase activity.
[0123] Examples of methionine synthase activity assays, in vitro
and in whole cells, are well-known to those skilled in the art
(see, for example, Gulati et al., 1997, J. Biol. Chem.
272:19171-19175; see also Rosenblatt et al., 1984, J. Clin. Invest.
74:2149-2156).
[0124] B. ELISA for the Detection of Compounds that Modulate
Methionine Synthase Reductase Expression
[0125] Enzyme-linked immunosorbant assays (ELISAs) are easily
incorporated into high-throughput screens designed to test large
numbers of compounds for their ability to modulate levels of a
given protein. When used in the methods of the invention, changes
in a given protein level of a sample, relative to a control,
reflect changes in the methionine synthase reductase expression
status of the cells within the sample. Protocols for ELISA may be
found, for example, in Ausubel et al., Current Protocols in
Molecular Biology, John Wiley & Sons, New York, N.Y., 1997.
Lysates from cells treated with potential modulators of methionine
synthase reductase expression are prepared (see, for example,
Ausubel et al., supra), and are loaded onto the wells of microtiter
plates coated with "capture" antibodies specific for methionine
synthase reductase. Unbound antigen is washed out, and a methionine
synthase reductase-specific antibody, coupled to an agent to allow
for detection, is added. Agents allowing detection include alkaline
phosphatase (which can be detected following addition of
colorimetric substrates such as p-nitrophenolphosphate),
horseradish peroxidase (which can be detected by chemiluminescent
substrates such as ECL, commercially available from Amersham) or
fluorescent compounds, such as FITC (which can be detected by
fluorescence polarization or time-resolved fluorescence). The
amount of antibody binding, and hence the level of a methionine
synthase reductase polypeptide within a lysate sample, is easily
quantitated on a microtiter plate reader.
[0126] As a baseline control for methionine synthase reductase
expression, a sample that is not exposed to test compound is
included. Housekeeping proteins are used as internal standards for
absolute protein levels. A positive assay result, for example,
identification of a compound that increases or decreases methionine
synthase reductase expression, is indicated by an increase or
decrease in methionine synthase reductase polypeptide within a
sample, relative to the methionine synthase reductase level
observed in cells which are not treated with a test compound.
[0127] C. Reporter Gene Assays for Compounds that Modulate
Methionine Synthase Reductase Expression
[0128] Assays employing the detection of reporter gene products are
extremely sensitive and readily amenable to automation, hence
making them ideal for the design of high-throughput screens. Assays
for reporter genes may employ, for example, colorimetric,
chemiluminescent, or fluorometric detection of reporter gene
products. Many varieties of plasmid and viral vectors containing
reporter gene cassettes are easily obtained. Such vectors contain
cassettes encoding reporter genes such as
lacZ/.beta.-galactosidase, green fluorescent protein, and
luciferase, among others. Cloned DNA fragments encoding
transcriptional control regions of interest (e.g. that of the
mammalian methionine synthase reductase gene) are easily inserted,
by DNA subcloning, into such reporter vectors, thereby placing a
vector-encoded reporter gene under the transcriptional control of
any gene promoter of interest. The transcriptional activity of a
promoter operatively linked to a reporter gene can then be directly
observed and quantitated as a function of reporter gene activity in
a reporter gene assay.
[0129] Cells are transiently- or stably-transfected with methionine
synthase reductase control region/reporter gene constructs by
methods that are well known to those skilled in the art. Transgenic
mice containing methionine synthase reductase control
region/reporter gene constructs are used for late-stage screens in
vivo. Cells containing methionine synthase reductase/reporter gene
constructs are exposed to compounds to be tested for their
potential ability to modulate methionine synthase reductase
expression. At appropriate timepoints, cells are lysed and
subjected to the appropriate reporter assays, for example, a
colorimetric or chemiluminescent enzymatic assay for
lacZ/.beta.-galactosidase activity, or fluorescent detection of
GFP. Changes in reporter gene activity of samples treated with test
compounds, relative to reporter gene activity of appropriate
control samples, indicate the presence of a compound that modulates
methionine synthase reductase expression.
[0130] D. Quantitative PCR of Methionine Synthase Reductase mRNA as
an Assay for Compounds that Modulate Methionine Synthase Reductase
Expression
[0131] The polymerase chain reaction (PCR), when coupled to a
preceding reverse transcription step (rtPCR), is a commonly used
method for detecting vanishingly small quantities of a target mRNA.
When performed within the linear range, with an appropriate
internal control target (employing, for example, a housekeeping
gene such as actin), such quantitative PCR provides an extremely
precise and sensitive means of detecting slight modulations in mRNA
levels. Moreover, this assay is easily performed in a 96-well
format, and hence is easily incorporated into a high-throughput
screening assay. Cells are treated with test compounds for the
appropriate time course, lysed, the mRNA is reverse-transcribed,
and the PCR is performed according to commonly used methods, (such
as those described in Ausubel et al., Current Protocols in
Molecular Biology, John Wiley & Sons, New York, N.Y., 1997),
using oligonucleotide primers that specifically hybridize with
methionine synthase reductase nucleic acid. Changes in product
levels of samples exposed to test compounds, relative to control
samples, indicate test compounds that modulate methionine synthase
reductase expression.
[0132] Secondary Screens of Test Compounds that Appear to Modulate
Methionine Synthase Reductase Activity
[0133] After test compounds that appear to have methionine synthase
reductase-modulating activity are identified, it may be necessary
or desirable to subject these compounds to further testing. At late
stages testing will be performed in vivo to confirm that the
compounds initially identified to affect methionine synthase
reductase activity will have the predicted effect in vivo. Such
tests may be performed using cells or animals that have wild-type,
mutated, or deleted methionine synthase reductase genes, or
wild-type or mutated methionine synthase reductase transgenes.
[0134] Therapy
[0135] Compounds identified using any of the methods disclosed
herein, may be administered to patients or experimental animals
with a pharmaceutically-acceptable diluent, carrier, or excipient,
in unit dosage form. Conventional pharmaceutical practice may be
employed to provide suitable formulations or compositions to
administer such compositions to patients or experimental animals.
Although intravenous administration is preferred, any appropriate
route of administration may be employed, for example, parenteral,
subcutaneous, intramuscular, intracranial, intraorbital,
ophthalmic, intraventricular, intracapsular, intraspinal,
intracisternal, intraperitoneal, intranasal, aerosol, or oral
administration. Therapeutic formulations may be in the form of
liquid solutions or suspensions; for oral administration,
formulations may be in the form of tablets or capsules; and for
intranasal formulations, in the form of powders, nasal drops, or
aerosols.
[0136] Methods well known in the art for making formulations are
found in, for example, "Remington's Pharmaceutical Sciences."
Formulations for parenteral administration may, for example,
contain excipients, sterile water, or saline, polyalkylene glycols
such as polyethylene glycol, oils of vegetable origin, or
hydrogenated naphthalenes. Biocompatible, biodegradable lactide
polymer, lactide/glycolide copolymer, or
polyoxyethylene-polyoxypropylene copolymers may be used to control
the release of the compounds. Other potentially useful parenteral
delivery systems for antagonists or agonists of the invention
include ethylene-vinyl acetate copolymer particles, osmotic pumps,
implantable infusion systems, and liposomes. Formulations for
inhalation may contain excipients, for example, lactose, or may be
aqueous solutions containing, for example, polyoxyethylene-9-lauryl
ether, glycocholate and deoxycholate, or may be oily solutions for
administration in the form of nasal drops, or as a gel.
[0137] The following examples are to illustrate, not limit the
invention.
EXAMPLE 1
General Methods
[0138] Materials
[0139] Radiolabeled compounds were from DuPont (Wilmington, Del.).
A human multiple tissue Northern blot and .beta.-actin probe were
from Clontech (Palo Alto, Calif.). The random-primed DNA labelling
kit was from Boehringer Mannheim (Indianapolis, Ind.). The T/A
cloning kit was from Invitrogen (Carlsbad, Calif.), the Geneclean
III kit was obtained from Bio101 Inc. (Vista, Calif.), and the
Wizard Mini-Preps were from Promega (Madison, Wis.). Taq
polymerase, AMV reverse transcriptase, Trizol reagent, and were
purchased from Gibco BRL (Gaithersburg, Md.), and restriction
enzymes were purchased from Gibco BRL and New England Biolabs
(Beverly, Mass.). The Sequenase kits for manual sequencing of crude
PCR products or plasmids were from United States Biochemicals
(Cleveland, Ohio). The oligonucleotides (SEQ ID NOs: 3-20 and
49-50) were synthesized by ACGT Corporation (Toronto, Canada) or by
the Sheldon Biotechnology Centre, McGill University. The sequences
of oligonucleotides are shown in Table 1 and in FIG. 2. A human
cDNA library, made in Lambda-ZAP from RNA derived from the human
colon carcinoma line Caco-2, was used as template in some PCR
reactions to obtain 5' extensions of the cDNA.
[0140] Homology Matches
[0141] Comparisons were made between putative FMN, FAD and NADPH
binding sites and sequences in the NCBI databases (dbEST and nr)
using the BLAST programs (Altschul et al., Nat. Genet. 6:119-129,
1994). The cytochrome P450 reductase and nitric oxide synthase full
sequences were also used for homology searching.
[0142] PCR Cloning and DNA Sequencing
[0143] Total cellular RNA was isolated by the method of Chirgwin et
al. (Biochemistry, 18:5294-5299, 1979) and reverse-transcribed
using oligo-dT15 as primer. PCR was conducted as described
previously (Triggs et al., Am. J. Hum. Genet. 49:1041-1054, 1991).
The PCR products were purified using Geneclean, subcloned in the
pCR2.1 vector and transformed into E. coli according to the
supplier's protocol (TA cloning kit). The resulting clones were
sequenced manually to confirm the specificity of PCR products.
Automated sequencing was done by Bio S&T Inc. (Montreal,
Canada) or by the DNA Sequencing Core Facility of the Canadian
Genetic Diseases Network.
[0144] Northern Blot
[0145] The multiple tissue Northern blot, prepared from poly(A)+
RNA (2 .mu.g/lane) of the indicated human tissues, was probed with
an EcoRI segment of a subclone in pCRII containing an insert
spanning positions 335-2148 of the methionine synthase reductase
cDNA. Hybridization with human .beta.-actin cDNA served as a
control for the quantity and integrity of the RNA in the blot.
[0146] Chromosomal Localization
[0147] We performed PCR analysis of DNA from the NIGMS human/rodent
somatic cells hybrid mapping panel (#2). The oligonucleotide
primers, which were specific for the 3'-UTR region of the gene,
amplified a 111 nucleotide product (accession #G19837 in dbSTS). A
P1-derived artificial chromosome (PAC) clone (104K2) was identified
from a total human genomic library (Ioannou, P. A. et al., Nat.
Genet. 6:84-89, 1994) by hybridization screening with a methionine
synthase reductase cDNA probe (clone 704947, accession #AA279726 in
dbEST) and this genomic clone was then used for FISH mapping (Heng,
H. H. et al., Proc. Natl. Acad. Sci. USA 89:9509-9513, 1992; Heng,
H. H and Tsui, L. C., Chromosoma 102:325-332, 1993).
[0148] Cell Lines
[0149] Ten fibroblast cell lines from patients with homocystinuria
(cblE complementation group) were used to identify mutations and
polymorphisms in the MTRR gene using reverse transcription-PCR of
total cellular RNA. Three of the cell lines displayed mutations:
WG788 from the original cblE patient (Schuh et al., N. Engl. J.
Med. 310:686-690, 1984); WG1146 from his younger brother, who had
been diagnosed before birth, and whose mother was treated with
hydroxocobalamin during pregnancy (Rosenblatt et al., Lancet
1:1127-1129, 1985); and WG1836 from a patient who had previously
been described as having dihydrofolate reductase deficiency (case 1
in Tauro et al., N. Engl. J. Med. 294:466, 1976) and subsequently
as having a "new mutation" associated with low methylcobalamin
levels and reduced cellular folate uptake (Brasch et al, Aust. N.
Z. J. Med. 18 Supp. 434, 1988). In our laboratory, we have shown
that the fibroblast line from this last patient falls into the cblE
complementation group.
[0150] The fibroblast cell line WG1401 was the first to show the
polymorphism, an A to G substitution at bp 66. WG1401 is from
patient B.S.S. 17, with megaloblastic anemia, hyperhomocysteinemia,
and mild methylmalonic aciduria. The polymorphism was also found in
a control cell line, MCH64.
[0151] Twenty-two other cell lines were used as normal controls for
mutation analysis.
[0152] Mutation Analysis by RT-PCR of Fibroblast RNA
[0153] Total cellular RNA was isolated from fibroblast pellets
(Chirgwin et al., Biochemistry, 18:5294-5299, 1979). It was reverse
transcribed using 25 .mu.g total RNA in reactions containing 2.5 U
of AMV reverse transcriptase and 500 ng of methionine synthase
reductase-specific terminal oligonucleotide 2101C (SEQ ID NO: 20;
Table 1) in a total reaction volume of 54 .mu.l. The resultant cDNA
was used as template for PCR. PCR for nine overlapping cDNA
segments was performed in reactions containing 3 .mu.l of template,
1 .mu.l each of dTTP, dGTP, dATP and dCTP (10 mM), and 3 U Taq
polymerase in a 46 .mu.l volume. PCR products were verified by
agarose gel electrophoresis before testing for heteroduplex
formation. Heteroduplex analysis was carried out by mixing mutant
and control PCR products 1:1, heating the mixture to 95.degree. C.
for 3 min, cooling to room temperature, and subjecting the samples
to electrophoresis on an 8% polyacrylamide gel. Fragments
displaying shifts were subcloned and sequenced, or sequenced
directly.
[0154] MMTR Polymorphism Analysis in Genomic DNA Samples
[0155] For the screening of genomic DNA samples, restriction
digestion analysis was performed with an artificially-created NdeI
restriction site using the sense primer MSG108S
5'GCAAAGGCCATCGCAGAAGACAT (SEQ ID NO: 49) and antisense primer
AD292 5'GTGAAGATCTGCAGAAAATCCATGTA (SEQ ID NO: 50), where the
underlined C replaces the A to generate an NdeI restriction site in
the normal sequence. To test for the mutation, 10 .mu.l of PCR
product was digested by adding 6 .mu.l H2O, 2 .mu.l New England
Biolab's (NEB) buffer 4 and 2 .mu.l NdeI. The PCR fragment of 66 bp
remains uncut in the presence of the G (methionine) allele, but is
digested into fragments of 44 bp and 22 bp in the presence of the A
(isoleucine) allele.
[0156] Subjects
[0157] Patients with spina bifida (n=56) and mothers of children
with spina bifida (n=58) were recruited from the Montreal
Children's Hospital after approval of the protocol by the
Institutional Review Board. The controls (n=97) were other
outpatients who were having a venipuncture at the Pediatric Test
Center, Montreal Children's Hospital, and who were with their
mothers (n=89). Blood samples were obtained from mothers and
children after appropriate consent. Exclusion criteria were
syndromic neural tube disorder (NTD) cases, severe anemia,
neoplastic disease, renal insufficiency and immunosuppressive
therapy. Individuals who were taking vitamin supplements were also
excluded. The methylenetetrahydrofolate reductase (MTHFR) genotypes
and the levels of plasma homocysteine and serum cobalamin were
previously determined in these subjects. The concentration of serum
cobolamin was quantitated by routine methods, using an automated
system and reagents from Ciba (Ciba Corning Diagnostics Corp.,
Medfield, Mass.).
[0158] To determine total homocysteine (tHcy) levels in plasma,
blood samples were drawn to Becton-Dickinson vacutainers containing
sodium EDTA and kept on ice until plasma was separated. Plasma was
separated by centrifugation for 5 min., removed, and cetrifuged
again; the supernatant was collected and frozen at -20.degree. C.
until analysis. tHcy in plasma was determined by high pressure
liquid chromatography as reported (Gilfix et al., Clin. Chem.
43:687-688, 1997). The tHcy adduct was detected by fluorescence
after precolumn derivitization with the thiol-specific reagent
7-fluoro-benzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F) (Wako,
USA).
[0159] To detect the MTHFR polymorphism, DNA was isolated from
peripheral leukocytes by extraction with phenol-chloroform after
cell lysis in a buffer containing Nonidet-P40 (Boehringer Mannheim,
Mannheim, Germany) and stored at -20.degree. C. The presence of the
677C.fwdarw.T polymorphism in MTHFR (SEQ ID NO: 51) was determined
by PCR followed by restriction digestion with HinfI, as described
(Frosst et al., Nat. Genet. 10:111-113, 1995).
[0160] Statistics
[0161] Computer-assisted statistical analyses were carried out
using SAS for Windows (Version 6.12). Standard summary statistics,
analysis of variance, t-tests, calculation of odds ratios with
associated confidence limits, and logistic regression models were
used where appropriate. Statistical significance was interpreted as
p-values of p<0.05.
EXAMPLE II
Cloning of the Human Methionine Synthase Reductase cDNA
[0162] More than 20 overlapping sequences homologous to the FAD and
NADPH-binding domains of cytochrome P450 reductase were identified
in an initial survey of the NCBI dbEST database using TblastN. We
sequenced clones 550341 (accession #AA085543), 704947 (accession
#AA279726) and 31776 (accession #R17835) to confirm the sequence of
this part of the cDNA. Reprobing the NCBI databases with this
sequence yielded a C. elegans sequence (accession #Z35595)
containing binding sites for FMN, FAD and NADPH. We then used the
C. elegans sequence to reprobe the dbEST database using TblastN and
identified a human sequence (accession #AA192690, clone 628497)
containing a putative FMN binding site similar to the one encoded
by Z35595. We designed a sense primer based on the FMN binding
region of AA192690 and antisense primers corresponding to the
FAD/NADPH binding regions of the methionine synthase reductase
candidate and amplified a sequence by RT-PCR using human
fibroblasts as the source of RNA. FIG. 2 shows the overlapping
clones and PCR fragments used to clone and sequence human
methionine synthase reductase. The EST clones are shown as
rectangles, the subsequences that were available from the dbEST
database are shown as hatched boxes, and the PCR fragments are
represented as lines. The oligonucleotide names are indicated below
the arrows in FIG. 2 and are described in Table 1 below. The primer
in parentheses designates a mispriming outcome that generated valid
internal sequence. The letter "V" in black boxes indicates primers
annealing to the vector of the cDNA library used as a template for
PCR. The presence of a triangle above a segment indicates that it
contained a deletion of 154 bp (open triangle) or 26 bp (black
triangle), likely caused by alternative splicing.
1TABLE 1 Oligonucleotides used for cDNA cloning, mapping, and
mutation detection. Primers Sequence Location Z116 (SEQ ID NO: 3)
5'-CTCCTGCTCGAACATCTTCCTAAA 1318-1341 Z117 (SEQ ID NO: 4)
5'-AATAGATAATCCCTATCCTTATGCC 1766-1742 AD150 (SEQ ID NO: 5)
5'-CCCTGGCTCCTAAGATATCCATC 1544-1566 AD151 (SEQ ID NO: 6)
5'-CGAACAACAAATTCTTTCCACTTACC 1573-1598 AB191 (SEQ ID NO: 7)
5'-CAAGGTTGGTGGAAGTCGCGTTG -79--57 AA468 (SEQ ID NO: 8)
5'-ATGCCTTGAAGTGATGAGGAGGTTT -13-12 AB586 (SEQ ID NO: 9)
5'-TTCCTACAACATAGAGAGAAACTC 1663-1686 AB588 (SEQ ID NO: 10)
5'-TTGCACAAGGGCATCATGTACA- TC 1998-1975 Z593 (SEQ ID NO: 11)
5'-AAACCTCCTCATCACTTCAAG- GCAT 12--13 Z594 (SEQ ID NO: 12)
5'-CTTGCACACGAATATGGTCTG- GG 1370-1348 Z596 (SEQ ID NO: 13)
5'-TGGCATCACCTGCATCCTTGA- GG 506-528 Z597 (SEQ ID NO: 14)
5'-GATGTACCTGTAAATATTCTGGG- GG 760-736 1103A (SEQ ID NO: 15)
5'-AATCCACGGCTCAACCACAAGT- TC 429-406 1761 (SEQ ID NO: 16)
5'-CTCGAAATTAACCCTCACTAAAG- GG in Bluescript 1803E (SEQ ID NO: 17)
5'-AACCCATACCGCAGGTGAGCAAA 278-256 1812B (SEQ ID NO: 18)
5'-TTTAGTACTTTCAGTCAAAAAAGCTTAAT 2148-2120 1902C (SEQ ID NO: 19)
5'-ATAAACGACTTCAAGAGCTTGGAGC 335-359 2101C (SEQ ID NO: 20)
5'-AGGTTTGGCACTAGTAAAGCTGACT 2173-2149 MSG108S (SEQ ID NO: 49)
5'-GCAAAGGCCATCGCAGAAGACAT 43-65 AD292 (SEQ ID NO: 50)
5'-GTGAAGATCTGCAGAAAATCCATGTA 83-108
[0163] The sequence of the PCR products confirmed that our cDNA
contained the putative FMN, FAD and NADPH binding sites. The 5' end
of the sequence was obtained by PCR using a cDNA library as
template, with antisense primers specific for the cDNA and a sense
primer that anneals to the vector used to construct the library.
The sequences generated by PCR were taken as error-free by
comparison of the sequence of at least two, and usually three,
independent PCR reactions.
[0164] The coding sequence of human methionine synthase reductase
contains 2094 bp (SEQ ID NO: 1 and SEQ ID NO: 41) encoding a
polypeptide of 698 amino acids (SEQ ID NO: 2 and SEQ ID NO: 42) in
length. FIG. 3 shows the cDNA sequence (SEQ ID NO: 24) and deduced
amino acid sequence of human methionine synthase reductase. The
nucleotide residues are numbered on the left margin, the amino
acids residues are numbered on the right margin, and the stop codon
is indicated by three stars. The sequence has been deposited in the
GenBank database, accession #AF025794.
[0165] The predicted MW of human methionine synthase reductase is
77,700. It shares 38% sequence identity (49% similarity) with human
cytochrome P450 reductase (accession #A60557) and 43% identity (53%
similarity) with the C. elegans putative methionine synthase
reductase (accession #Z35595). FIG. 4 shows amino acid sequence
comparisons among human methionine synthase reductase (HsMTRR), C.
elegans putative methionine synthase reductase (CeMTRR) and human
cytochrome P450 reductase (HsCPR). The amino acids residues are
numbered on the right margin, and conserved residues are shown by
stars under the sequence. Alignments of similar amino acids are
dotted (A, G, S, T; D, E, N, Q; V, L, I, M; K, R; and F, W, Y), and
regions proposed to be involved in binding of FMN, FAD or NADPH are
shown above the sequences.
[0166] The first in-frame methionine residue is a candidate for the
initiation codon. It is perfectly aligned with the first methionine
of the C. elegans sequence, and the presence of a G at positions -3
and -6 places the sequence in good context for initiation of
translation (Kozak, J. Biol. Chem. 266:19867-19870, 1991). A
polyadenylation signal is present at positions 3135-3140. The
poly(A) tail is added after position 3165, although we observed
some clones with polyadenylation after residue 3157.
[0167] RT-PCR involving various pairs of primers allowed us to
detect alternatively processed methionine synthase reductase mRNA,
including one form with a deletion of 154 bp (nucleotides 129-282)
and another lacking a 26 bp segment (-52 to -27), accounting for
less than 20% and 40% of the mRNA, respectively.
EXAMPLE III
Expression of Human Methionine Reductase mRNA
[0168] A PCR product generated with primers 1902C (SEQ ID NO: 19)
and 1812B (SEQ ID NO: 18) was subcloned and used to probe a
Northern blot prepared from several human tissues.
[0169] FIGS. 5A and 5B show a Northern blot analysis of methionine
synthase reductase expression in human tissues, with the positions
of the molecular size (kb) markers indicated at the left. The 1.8
kb probe hybridized to one predominant RNA species of 3.6 kb.
Methionine synthase reductase appears to be expressed to some
degree in all tissues tested and is particularly abundant in
skeletal muscle. In addition to the 3.6 kb band, a 3.1 kb band and
a faint 6 kb band were detected in brain mRNA.
EXAMPLE IV
Chromosomal Mapping of the Human Methionine Synthase Reductase
Gene
[0170] The methionine synthase reductase gene was localized to
human chromosome 5, since the gene-specific primer pair amplified a
PCR product of the expected size only from the GM 10114 hybrid,
which contains chromosome 5 as its only human material. Moreover,
the DNA sequence we determined for the methionine synthase
reductase gene matched markers AA002A03 and STSG444, which were
also mapped by the NCBI consortium to chromosome 5 between markers
D5S406-D5S478 and D5S406-D5S635, respectively (Hudson, T. J. et
al., Science 270:1945-1954, 1995). To determine the cytogenetic
position of the gene on chromosome 5, we mapped a genomic PAC clone
encompassing the gene using fluorescence in situ hybridization
(FISH). FIG. 6 shows a summary of the FISH mapping of the
methionine synthase reductase gene to human chromosome
5p15.2-p15.3. Each dot represents a signal detected on human
chromosome 5. The hybridization efficiency was 100%, and, among 100
mitotic figures examined, each result indicated that the gene was
located on chromosome 5p15.2-p15.3. We propose MTRR as the gene
name for methionine synthase reductase, since the methionine
synthase gene has been named MTR.
EXAMPLE V
Mutations of the Methionine Synthase Reductase Gene in Patients of
the cblE Complementation Group
[0171] To confirm the identity of the candidate cDNA as methionine
synthase reductase, patient cell lines from the cblE
complementation group were analyzed by RT-PCR-dependent
heteroduplex analysis using nine RT-PCR reactions that yielded
overlapping products, in order to cover the length of the candidate
cDNA sequence. Patient samples were mixed with RT-PCR product from
normal cells to ensure the availability of wild-type DNA, in order
to enable the detection of heteroduplexes in samples in which the
mutation might be homozygous. For samples yielding heteroduplexes,
the analysis was repeated without prior mixing with wild-type DNA,
in order to determine whether the relevant changes were
heterozygous. Three cell lines showed typical heteroduplex
patterns, one of them observed in overlapping RT-PCR fragments
(FIGS. 7A and 7B).
[0172] FIGS. 7A and 7B show a mutation analysis of the methionine
synthase reductase gene in cblE patient cell lines. FIG. 7A shows
the PCR products obtained with primers Z116 (SEQ ID NO: 3) and Z117
(SEQ ID NO: 4) from RT reactions with control sample (WT) and two
cblE cell lines, WG1146 and WG1836. The bands above the 449 bp
amplification product result from heteroduplexes formed between DNA
strands bearing different allelic sequences. The pattern observed
for cell line WG1146 was also seen with cell line WG788 (the
sibling of WG1146). FIG. 7B shows RT-PCR products amplified with
primers. AB586 (SEQ ID NO: 9) and AB588 (SEQ ID NO: 10) from a
control sample and cell line WG1836. Heteroduplexes are observed
above the 336 bp band for cell line WG1836.
[0173] The heteroduplex-containing samples were subcloned and
sequenced and two mutations were identified. A heterozygous
mutation present in fibroblast line WG788 is a 4 bp deletion,
1675del4, resulting in a frameshift that creates a nearby stop
codon. The same mutation was observed in cell line WG1146 from the
brother of patient WG788. Direct sequencing of the PCR product
using primer AD150 showed overlapping sequences starting at
position 1675, consistent with the heterozygous presence of the 4
bp deletion.
[0174] The second heterozygous mutation, detected in cell line
WG1836, is an in-frame deletion of 3 bp, 1726delTTG. It results in
the loss of a highly conserved leucine at position 576 of the amino
acid sequence.
[0175] FIG. 7C shows a sequence comparison among proteins of the
FNR family in a part of the NADPH binding region in the vicinity of
the leucine residue that is deleted in a cblE patient (denoted by a
triangle; MTRR is methionine synthase reductase; CPR is cytochrome
P450 reductase; NOS is nitric oxide synthase; SR is sulfite
reductase; and FNR is NADPH-ferredoxin (flavodoxin) reductase).
[0176] Primer AD151 (SEQ ID NO: 6) was used for direct sequencing
of the WG1836 PCR product. In this case, the deletion of
nucleotides 1726-1728 was clearly visible. There was only a very
faint background contributed by the normal sequence, suggesting
that a second, unidentified mutation in this cell line was
associated with a very low level of steady-state mRNA.
EXAMPLE VI
Human Methionine Synthase Reductase Polymorphisms
[0177] We have identified two polymorphisms in methionine synthase
reductase cDNAs. The first is a G/A polymorphism at nucleotide
position 66, using the "A" of the initiator methionine as
nucleotide position number 1 (see FIG. 3), which results in either
an isoleucine or a methionine, respectively, at amino acid 22. The
second polymorphism is a G/A polymorphism at nucleotide position
110, which results in either a tyrosine or a cysteine,
respectively, at amino acid position 37. It is likely that
additional methionine synthase reductase polymorphisms will be
found, some of which will be associated with increased or decreased
risks of disease.
EXAMPLE VII
A Common Polymorphism in Methionine Synthase Reductase as a Risk
Factor for Spina Bifida
[0178] During screening for methionine synthase reductase (MTRR)
mutations in patients with homocystinuria, we identified an A/G
polymorphism at bp 66, which yields an isoleucine (22I) or a
methionine (22M), respectively, at amino acid position 22 (FIG.
8A). Since the presence of the methionine polymorphism at this
position did not create or obliterate a naturally-occurring
restriction site, a PCR-dependent diagnostic test was established
that makes use of a modified sense primer to create a NdeI site in
the isoleucine allele during the amplification reaction. The PCR
product of 66 bp remains uncut in the presence of the methionine
allele, but is digested into fragments of 44 and 22 bp in the
presence of the isoleucine allele (FIG. 8B). The cDNA sequence
reported in Leclerc, et al., Proc. Natl. Acad. Sci. USA,
95:3059-3064, 1998, contained the methionine codon.
[0179] The NdeI assay was used to assess allele frequencies in
controls. The 22I/22M polymorphism was extremely common in our
control adult population (mothers of control children, n=89).
Forty-nine percent were heterozygous while 26% were homozygous for
the methionine allele (Table 2). The allele frequency was 0.51 for
the methionine variant. Similar frequencies were observed for
control children. The controls in this study were white Caucasian
individuals with French, British, and mixed European ancestry.
Since the allele frequency is virtually identical for the two
variants, the designation of a "wild type" allele could not be
ascertained based on frequency. However, this gene has significant
homology with related FMN-binding proteins from other organisms,
including the putative methionine synthase reductase from C.
elegans, as well as sulfite reductases, nitric oxide synthases,
cytochrome P450 reductases, and flavodoxins. The equivalent codon
in these genes is isoleucine, leucine, or valine in 123 out of 130
entries in GenBank. None of the entries contained a methionine
codon. Consequently, the ancestral human MTRR sequence is likely to
contain the isoleucine codon (22I), with a subsequent mutation to
methionine (22M).
[0180] In this study, 34% (19/56) of case (spina bifida) children
and 36% (21/58) of case mothers were homozygous for the 22M
polymorphism in MTRR, compared to 30% (29/97) of control children
and 26% (23/89) of control mothers (Table 2). An increased risk for
being a case (odds ratio (OR) 1.7, 95% confidence interval (CI)
0.67-4.6)) or a case mother (O.R. 2.0, 95% CI 0.77-5.2) was
observed when the homozygous mutant (M/M) genotype was present, but
this increase was not statistically significant. Mother-child
genotype pairs were also assessed for neural tube defect (NTD) risk
to determine if the combination of mutant maternal and mutant child
genotypes conferred a greater risk than either genotype alone; an
increased risk was not observed. Homocysteine levels were not
increased in individuals who were homozygous mutant for MTRR (Table
3).
[0181] Synergistic Interaction Between MTRR Genotype and Cobalamin
Level Influences the Risk of NTD
[0182] Case children had serum cobalamin levels (pmol/L) of
487.+-.250 (n=55), whereas control children had serum cobalamin
levels of 535.+-.339 (n=95); case mothers had serum cobalamin
levels of 298.+-.186 (n=59), whereas control mothers had serum
cobalamin levels of 350.+-.135 (n=88; p=0.05). We therefore asked
whether the mutant MTRR genotype may have a greater impact on NTD
risk when cobalamin levels are low. Table 4 shows the results of
multiple logistic regression analysis, adjusted for age, to test
this hypothesis. Having a cobalamin level in the lowest quartile of
the control distribution was associated with a nonsignificant
two-fold increase in risk for the case mothers (O.R.=2.1; 95%
CI=0.86-5.2). There was no increase in risk for low cobalamin in
the children. However, the combination of homozygous mutant
genotype and low cobalamin was associated with a significant 5-fold
increase in risk for the mothers, compared to those without the M/M
genotype and with cobalamin levels in the other 3 quartiles
(O.R.=4.8, 95% CI=1.5-15.8). The risk for the children with this
combination was also increased but statistical significance was not
observed (O.R.=2.5, 95% CI=0.63-9.7). There was no increased risk
for the mutant genotype combined with low folate. Because the MTRR
genotype alone was associated with less risk, we speculate that
genotype and cobalamin levels work in unison to produce increased
risk for spina bifida in the case mothers and case children.
[0183] Synergistic Interaction Between MTRR and MTHFR Genotypes
Influences the Risk of NTD
[0184] The 677C.fwdarw.T polymorphism (SEQ ID NO: 51) in the
methylenetetrahydrofolate reductase (MTHFR) gene converts an
alanine to a valine residue in the enzyme (Frosst et al., Nat.
Genet. 10:111-113, 1995). MTHFR catalyzes the synthesis of
5-methyltetrahydrofolate, the primary circulatory form of folate
and the methyl donor in the remethylation of homocysteine to
methionine by methionine synthase. Several studies have
demonstrated an increased frequency of the homozygous mutant (V/V)
MTHFR genotype in children with NTDs and in their mothers (van der
Put et al., Lancet 346:1070-1071, 1995; Whitehead et al., Quart. J.
Med. 88:763-766, 1995; Ou et al., Am. J. Med. Genet. 63:610-614,
1996).
[0185] Table 5 shows the interaction between the MTRR genotype and
the MTHFR genotype in NTD risk, as determined by multiple logistic
regression analysis, adjusted for age. Using a genotype of either
homozygous wild type or heterozygous for MTRR and homozygous wild
type for MTHFR as the reference, a risk nearly five times as great
is conferred to case children (O.R.=4.9, 95% CI=1.1-21.8) and to
case mothers (O.R.=5.0, 95% CI 0.8-31.3) when they are homozygous
for both mutations. The risk for the combination of mutant
genotypes is clearly higher than either mutant genotype alone, in
both the cases and in their mothers.
2TABLE 2 Frequency of MTRR genotypes in children with spina bifida
(cases) and in case mothers. I/I I/M M/M Cases 9/56 (16%) 28/56
(50%) 19/56 (34%) Controls 24/97 (25%) 44/97 (45%) 29/97 (30%) Case
mothers 10/58 (17%) 27/58 (47%) 21/58 (36%) Control mothers 22/89
(25%) 44/89 (49%) 23/89 (26%) O.R. for children, M/M vs. I/I = 1.7
(95% C.I. 0.67-4.6) O.R. for mothers, M/M vs. I/I = 2.0 (95% C.I.
0.77-5.2)
[0186]
3TABLE 3 Homocysteine levels stratified by MTRR genotype. (tHcy
(.mu.mol/L)) I/I I/M M/M n n n Children 7.7 .+-. 2.8 8.2 .+-. 3.3
8.2 .+-. 3.1 33 72 48 Mothers 9.7 .+-. 2.8 10.3 .+-. 4.7 9.4 .+-.
3.1 32 71 43
[0187]
4TABLE 4 Logistic regression analysis for NTD risk in children and
mothers. Odds ratio > (95% C.I.) Cobalamin MTRR Genotype level
Children Mothers I/I or I/M normal 1.0 (ref.) 1.0 (ref.) I/I or I/M
low 0.92 (0.37-2.3) 2.1 (0.86-5.2) M/M normal 1.1 (0.46-2.5) 1.5
(0.56-4.1) M/M low 2.5 (0.63-9.7) 4.8 (1.5-15.8) Odds ratios are
adjusted by age of children and mothers respectively. Low cobalamin
refers to the lowest quartile of the control distribution; normal
refers to the other 3 quartiles.
[0188]
5TABLE 5 Logistic regression analysis for NTD risk in children and
mothers. Odds ratio > (95% C.I.) MTHFR MTRR Genotype Genotype
Children Mothers I/I or I/M A/A 1.0 (ref.) 1.0 (ref.) I/I or I/M
V/V 0.82 (0.18-3.7) 2.4 (0.69-8.3) M/M A/A 1.2 (0.34-4.5) 1.9
(0.61-5.7) M/M V/V 4.9 (1.1-21.8) 5.0 (0.80-31.3) Odds ratios are
adjusted by age of children and mothers respectively.
EXAMPLE VIII
Human Methionine Synthase Reductase Mutations and Polymorphisms in
Disease
[0189] Alterations in metabolism of folates, homocysteine,
methionine, vitamin B12, and S-adenosylmethionine are associated
with diseases such as megaloblastic anemia and conditions such as
hyperhomocysteinemia. In turn, hyperhomocysteinemia may be
associated with a higher than normal risk for cardiovascular
disease and neural tube defects. In addition, decreased folate
levels may be predictive of a lower than normal risk for
cancer.
[0190] DNA samples from patients having a disease or developmental
defect, such as those mentioned above, are analyzed for mutations
within the methionine synthase reductase coding region and/or
transcriptional control regions, and serum folate, red blood cell
folate, plasma homocysteine, and serum cobalamin levels are
measured. Patient samples are compared to control samples.
[0191] The cloning of the methionine synthase reductase gene makes
possible the determination of whether discrete mutations and
polymorphisms in methionine synthase reductase nucleic acid confer
an increased risk for, or in contrast, protection against, diseases
and conditions such as cardiovascular disease, cancer, and neural
tube defects, (those of skill in the art will understand that
polymorphisms and mutations may either increase or decrease the
relative risk of any given disease or developmental defect). This
collection of data in turn makes possible the development of
diagnostic assays that predict whether a subject has a higher than
normal risk of developing a disease or of having offspring with
developmental defects. An understanding of disease-enhancing or
-protective mutations allows the development of therapeutics that
appropriately modulate methionine synthase reductase activity.
EXAMPLE IX
Association Between Variants in MTHFR and/or MTRR with Down'S
Syndrome
[0192] We have identified an association between the identified
polymorphism in methionine synthase reductase (MTRR) (an A.fwdarw.G
polymorphism at nucleotide position 66), the identified
polymorphism in methylenetetrahydrofolate reductase (MTHFR) (a
C.fwdarw.T polymorphism at nucleotide 677 (SEQ ID NO: 51) that
converts an alanine to a valine residue (Frosst et al., supra)) and
Down's Syndrome. In the study presented in Table 6, the genotypes
of mothers of Down's Syndrome babies (DS mother) were compared to
the genotypes of mothers of normal babies. We found that mothers of
Down's Syndrome babies had a significant 2.49-fold greater
likelihood of having a homozygous mutation for the A.fwdarw.G
polymorphism at nucleotide position 66. In addition, we found that
mothers of Down's Syndrome babies had a 2.07 fold greater
likelihood of having a heterozygous mutation or a homozygous
mutation in the MTHFR gene. Finally, we identified a positive
interaction between the MTRR and MTHFR gene mutations. Table 6
demonstrates that mothers with Down's Syndrome babies had an even
greater likelihood of having both the MTRR and MTHFR mutations than
having either the MTRR or MTHFR mutations alone. Mothers with
Down's Syndrome babies had a 3.71 fold greater likelihood of having
both a MTRR and a MTHFR mutation than control mothers. This result
indicates that the identified mutations are useful as genetic
markers for detection of Down's Syndrome in a fetus or embryo.
Alternatively, these mutations can be used to assess the risk of a
particular mother of having a Down's Syndrome baby.
EXAMPLE X
Increased Risk for Premature Coronary Artery Disease
[0193] We investigated whether an A66G polymorphism in the MTRR
gene is associated with altered levels of homocysteine and/or the
risk of developing premature coronary artery disease. The findings
described below suggest that the methionine synthase reductase
homozygous GG genotype is a risk factor for the development of
premature coronary artery disease (Relative risk 1.49; 95% CI:
1.10-2.03), by a mechanism independent of the detrimental vascular
effects of hyperhomocysteinemia.
[0194] Four hundred seventy eight Caucasian individuals undergoing
cardiac catheterization procedures at the Carolinas Heart Institute
were recruited into the study. All patient volunteers provided
blood samples for the isolation of serum, plasma and DNA. Of the
478 consenting participants, 463 had complete MTRR genotype data
(96.86%), and 180 of these patients were at risk for premature
coronary artery disease (CAD) by having ages<58 years (38.88%).
A total of 124 of these individuals at risk (66.67%) had premature
coronary artery disease (CAD) with significant atherosclerosis,
defined as .gtoreq.50% occlusion of .gtoreq.1 major artery or
20-50% occlusions in each of >2 major arteries. The remaining 62
individuals (33.33%) were free of significant occlusions (<50%
occlusion in .ltoreq.1 major artery), and therefore were defined as
controls. Among the individuals with premature CAD 21/124 (16.94%)
were female and 103/124 (83.06%) were male. Of the 116/180
age-eligible study participants reporting ethnicity (64.44%), the
majority were of British or German descent. A summary of the
characteristics of the population of individuals<58 years of age
is presented in Table 7.
[0195] Arterial blood was collected in ACD (acid-citrate-dextrose)
and serum vacutainer tubes (Beckton-Dickinson, Franklin Lanes,
N.J.) and immediately placed on ice (for <2 hours) prior to
centrifugation at 3000 rpm for 20 minutes. Plasma and serum were
removed and stored in screw cap cryovials at 70.degree. C. Plasma
homocysteine, serum folate, and vitamin B.sub.12 concentrations
were measured at the Vascular Disease Intervention and Research
Laboratory at the Oklahoma University Health Science Center.
[0196] The region surrounding the MTRR 66A.fwdarw.G polymorphism
was amplified by the polymerase chain reaction using primers
A:5'-CAGGCAAAGGCCATCGCAGAAGACAT-3' (SEQ ID NO: 61) and
B:5'-CACTTCCCAACCAAAATTCTTCAAAAG-3' (SEQ ID NO: 62). The
amplifications were performed in a 50 .mu.l volume, containing 200
ng genomic DNA, 10 mM TRIS, pH 8.8, 50 mM KCl, 1 mM each dNTP, 1
.mu.M each primer and 2.5 U Taq polymerase (Perkin-Elmer, Norwak,
Conn.). PCR cycling conditions in a GeneAmp 2400 thermal cycler
(Perkin-Elmer, Norwak, Conn.) were: 94.degree. C. for 5 minutes,
followed by 30 cycles of 94.degree. C. for 0.5 minutes, 55.degree.
C. for 0.5 minutes, 72.degree. C. for 0.5 minutes, and a final
extension of 72.degree. C. for 5 minutes. Primer A, containing a
mismatch from the MTRR sequence (underlined) creates a NdeI site in
the amplified DNA from alleles containing the 66A.fwdarw.G
polymorphism, digesting the 150 bp amplimer into 123 and 27 bp
fragments. The fragments were separated on a 2% Nusieve/1% agarose
gel containing 0.6 .mu.g/ml ethidium bromide.
[0197] Statistical analyses were performed using Stata Statistical
Software (College Station, Tex.). Contingency table analysis with
3-levels of genotype were used for comparison of disease or
genotype frequencies between groups, with sided p-values from
Pearson chi-square tests or from Fisher's Exact Test where expected
cell frequencies were .ltoreq.5, trend tests from Cuzick's
non-parametric test (Cuzick J. A, Statistics in Medicine (1985)
4:87-90) and non-parametric adjustments of relative risks with the
Mantel-Haenszel procedure. Kruskal-Wallace tests or analysis of
variance of lag transformed measurements was used to test for
differences in plasma tHcy, and serum folate and vitamin B.sub.12
levels between the three MTRR genotype levels, respectively.
Logistic regression was used to model risk of premature CAD
adjusted for other covariates. Descriptive statistics including
means and standard deviations or counts and percentages were
calculated. A p-value of less than 0.05 was considered
statistically significant.
[0198] We found that MTRR genotype analysis from 58 healthy,
unselected Caucasian individuals from North Carolina revealed a
genotype distribution of 22.4% AA, 50% AG, and 27.6% GG, indicating
the high frequency of the 66A.fwdarw.G polymorphism in the local
population.
[0199] We then determined if the MTRR genotype was associated with
premature CAD in our population of patients undergoing cardiac
catheterization procedures. A 3.times.2 contingency table analysis
displayed an association between premature CAD and both male sex
and MTRR genotype (p<0.0001) (Table 8). Among both males and
females, individuals with the homozygous GG genotype were at
greatest risk of developing premature CAD. Relative risks (RR) of
premature CAD, with Mantel-Haenszel adjustment for sex, were
RR=1.49 (95% CI: 1.10, 2.03) for GG versus AA and RR=1.21 (95% CI:
0.88, 1.65) for AG versus AA. Cuzick's non-parametric test for
trend in premature CAD risk across the ordered genotype groups
yielded a p-value of p=0.03.
[0200] A stratified analysis detected no appreciable modification
of the association between MTRR GG genotype and premature CAD by
MTHFR TT genotype, with a Mantel-Haenszel adjustment relative risk
for premature CAD for the MTRR GG versus MTRR AA genotypes across
MTHFR strata of 1.47 (95% CI: 1.04-2.06) compared to the crude
relative risk of premature CAD of 1.38 for the MTRR GG versus MTRR
AA genotypes.
[0201] As summarized in Table 9, no substantial differences in the
mean fasting plasm tHcy, serum folate, or vitamin B.sub.12
concentrations between the three MTRR genotype levels were
detected. Non-parametric Kruskal-Wallis tests for difference in the
distributions of continuous covariates yielded p-values of 0.65 for
tHcy, 0.18 for folate, and 0.69 for vitamin B.sub.12. ANOVA models
predicting log-transformed continuous variables with adjustment for
sex yielded p-values for MTRR level of 0.62 for tHcy, 0.25 for
folate, and 0.79 for vitamin B.sub.12.
[0202] We next examined the influence of vitamin B.sub.12 status on
the association between MTRR genotype and premature CAD as well as
with homocysteine level. The proportion of individuals with
premature CAD within the three MTRR genotype groups did not differ
among those with vitamin B.sub.12 levels above and below the median
value of 300 pg/mL (AA-58.8% versus 55.6%, n=35; AG-63.8% versus
67.5%, n=87; GG-77.3% versus 80.8%, n=48). The overall p-value for
premature CAD by vitamin B.sub.12 levels above and below the median
was 0.91, and adjustment for sex and MTRR level vial logistic
regression yielded a p-value for vitamin B.sub.12 levels above and
below the median of 0.79.
[0203] When combining case and control individuals, those with
B.sub.12 values below the median were found to have higher tHcy
concentrations (p=0.003). Individuals with B.sub.12 values below
the median had higher tHcy concentrations within each stratum of
MTRR genotype. The differences in .mu.mol/L and p-values from
Wilcox on rank sum tests for AA, AG and GG genotypes were 1.3
(p=0.049), 1.5 (p=0.031), and 1.6 (p=0.35), respectively.
[0204] The MTRR GG genotype was significantly associated with
premature onset coronary artery disease in the study population.
This association of genotype with disease was not modulated by
vitamin B.sub.12 status or MTHFR genotype. Without limiting the
biochemical mechanism of the invention, we propose that the
mechanism by which possession of the GG genotype predisposes a
subject to CAD does not appear to be related to the effects of
hyperhomocyteinemia, as there was no difference in tHcy
concentrations between the MTRR genotype levels. An inverse
relationship between vitamin B.sub.12 concentration and tHcy levels
was detected within the MTRR genotype groups, supporting previous
reports of an inverse relationship between homocysteine and vitamin
B.sub.12 levels (Verhoef et al. Am. J. Epidenm (1996) 143:845-859;
Folsom et al., Circulation (1998) 98:204-210).
[0205] These results indicate that the identified mutations are
useful as genetic markers for detection of premature cardiovascular
disease in a fetus or embryo. Alternatively, these mutations can be
used to assess the risk of a particular mother of having a baby
that might, in the future, develop premature cardiovascular
disease.
6TABLE 6 Association between variants in MTHFR or MTKR or both with
Down's Syndrome (DS) Interaction between MTHFR and MTRR gene
mutations MTHFR MTRR control DS mother Odds ratio (95% CI) - - 55
29 1 (reference) + - 59 65 2.07 (1.17-3.66) - + 15 20 2.49
(1.12-5.52) + + 19 38 3.71 (1.84-7.51) Total 148 152 MTHFR- =
Homozygous normal MTHFR+ = Heterozygous mutation and homozygous
mutation combined MTRR- = Homozygous normal and heterozygous
mutation combined MTRR+ = Homozygous mutation
[0206]
7TABLE 7 Comparison of characteristics among cases with coronary
artherosclerosis versus control subjects. Individuals <58 years
of age (n = 180) Patients Controls Sex - Male 99/119 (83.2%) 32/61
(52.5%) P < 0.001 -Female 20/119 (16.8%) 29/61 (47.5%)
Hypercholesterolemia 84/114 (73.7%) 33/59 (55.9%) P = 0.03
Hypertension 69/117 (59.0%) 33/60 (55.0%) P = 0.63 Diabetes 26/118
(22.0%) 8/61 (13.1%) P = 0.17 Current smoker 36/119 (30.3%) 15/61
(24.6%) P = 0.49
[0207]
8TABLE 8 Percentage of males and females <58 years of age with
CAD by MTRR genotype. MTRR Males Females Genotype Cases Controls
Cases Controls AA 64.5% 35.5% 16.7% 83.3% (n = 20) (n = 11) (n = 1)
(n = 5) AG 73.4% 26.6% 39.3% 60.7% (n = 47) (n = 17) (n = 11) (n =
17) GG 88.9% 11.1% 53.3% 46.7% (n = 32) (n = 4) (n = 8) (n = 7)
Cuzick's non-parametric test for trend in premature CAD risk across
the ordered genotype groups yielded a p-value of p = value of p =
0.03. RR of premature CAD = 1.49 (95% CI: 1.10, 2.03) for GG versus
AA.
[0208]
9TABLE 9 Distribution of tHey, folate and vitamin B.sub.12
concentrations by sex and MTRR genotype. MTRR AA MTRR AG MTRR GG
Male Female Male Female Male Female Plasma 11.0 .+-. 2.3 10.0 .+-.
3.0 12.2 .+-. 4.6 9.5 .+-. 3.1 11.1 .+-. 4.4 9.8 .+-. 2.8 tHcy
(.mu.mol/L) (n = 30) (n = 6) (n = 63) (n = 28) (n = 35) (n = 15)
Serum 16.3 .+-. 8.4 13.3 .+-. 7.5 13.4 .+-. 7.8 13.7 .+-. 9.5 14.0
.+-. 7.1 14.1 .+-. 5.2 folate (ng/mL) (n = 31) (n = 6) (n = 64) (n
= 28) (n = 35) (n = 15) Serum 338.1 .+-. 153.1 260.4 .+-. 66.4
350.8 .+-. 192.1 334.4 .+-. 176.6 320.4 .+-. 132.8 289.4 .+-. 88.7
vitamin B.sub.12 (pg/mL) (n = 30) (n = 5) (n = 59) (n = 28) (n =
35) (n = 13) p-values of 0.65 for tHcy, 0.18 for folate, and 0.69
for vitamin B.sub.12 (Kruskal-Wallis tests); p-values for 0.62 for
tHcy, 0.25 for folate, and 0.79 for vitamin B.sub.12 (ANOVA models
predicting log-transformed continuous variables with adjustment for
sex)
[0209]
Sequence CWU 1
1
63 1 2097 DNA Homo sapiens 1 atgaggaggt ttctgttact atatgctaca
cagcagggac aggcaaaggc catcgcagaa 60 gaaatgtgtg agcaagctgt
ggtacatgga ttttctgcag atcttcactg tattagtgaa 120 tccgataagt
atgacctaaa aaccgaaaca gctcctcttg ttgttgtggt ttctaccacg 180
ggcaccggag acccacccga cacagcccgc aagtttgtta aggaaataca gaaccaaaca
240 ctgccggttg atttctttgc tcacctgcgg tatgggttac tgggtctcgg
tgattcagaa 300 tacacctact tttgcaatgg ggggaagata attgataaac
gacttcaaga gcttggagcc 360 cggcatttct atgacactgg acatgcagat
gactgtgtag gtttagaact tgtggttgag 420 ccgtggattg ctggactctg
gccagccctc agaaagcatt ttaggtcaag cagaggacaa 480 gaggagataa
gtggcgcact cccggtggca tcacctgcat ccttgaggac agaccttgtg 540
aagtcagagc tgctacacat tgaatctcaa gtcgagcttc tgagattcga tgattcagga
600 agaaaggatt ctgaggtttt gaagcaaaat gcagtgaaca gcaaccaatc
caatgttgta 660 attgaagact ttgagtcctc acttacccgt tcggtacccc
cactctcaca agcctctctg 720 aatattcctg gtttaccccc agaatattta
caggtacatc tgcaggagtc tcttggccag 780 gaggaaagcc aagtatctgt
gacttcagca gatccagttt ttcaagtgcc aatttcaaag 840 gcagttcaac
ttactacgaa tgatgccata aaaaccactc tgctggtaga attggacatt 900
tcaaatacag acttttccta tcagcctgga gatgccttca gcgtgatctg ccctaacagt
960 gattctgagg tacaaagcct actccaaaga ctgcagcttg aagataaaag
agagcactgc 1020 gtccttttga aaataaaggc agacacaaag aagaaaggag
ctaccttacc ccagcatata 1080 cctgcgggat gttctctcca gttcattttt
acctggtgtc ttgaaatccg agcaattcct 1140 aaaaaggcat ttttgcgagc
ccttgtggac tataccagtg acagtgctga aaagcgcagg 1200 ctacaggagc
tgtgcagtaa acaaggggca gccgattata gccgctttgt acgagatgcc 1260
tgtgcctgct tgttggatct cctcctcgct ttcccttctt gccagccacc actcagtctc
1320 ctgctcgaac atcttcctaa acttcaaccc agaccatatt cgtgtgcaag
ctcaagttta 1380 tttcacccag gaaagctcca ttttgtcttc aacattgtgg
aatttctgtc tactgccaca 1440 acagaggttc tgcggaaggg agtatgtaca
ggctggctgg ccttgttggt tgcttcagtt 1500 cttcagccaa acatacatgc
atcccatgaa gacagcggga aagccctggc tcctaagata 1560 tccatctctc
ctcgaacaac aaattctttc cacttaccag atgacccctc aatccccatc 1620
ataatggtgg gtccaggaac cggcatagcc ccgtttattg ggttcctaca acatagagag
1680 aaactccaag aacaacaccc agatggaaat tttggagcaa tgtggttgtt
ttttggctgc 1740 aggcataagg atagggatta tctattcaga aaagagctca
gacatttcct taagcatggg 1800 atcttaactc atctaaaggt ttccttctca
agagatgctc ctgttgggga ggaggaagcc 1860 ccagcaaagt atgtacaaga
caacatccag cttcatggcc agcaggtggc gagaatcctc 1920 ctccaggaga
acggccatat ttatgtgtgt ggagatgcaa agaatatggc caaggatgta 1980
catgatgccc ttgtgcaaat aataagcaaa gaggttggag ttgaaaaact agaagcaatg
2040 aaaaccctgg ccactttaaa agaagaaaaa cgctaccttc aggatatttg gtcataa
2097 2 698 PRT Homo sapiens 2 Met Arg Arg Phe Leu Leu Leu Tyr Ala
Thr Gln Gln Gly Gln Ala Lys 1 5 10 15 Ala Ile Ala Glu Glu Met Cys
Glu Gln Ala Val Val His Gly Phe Ser 20 25 30 Ala Asp Leu His Cys
Ile Ser Glu Ser Asp Lys Tyr Asp Leu Lys Thr 35 40 45 Glu Thr Ala
Pro Leu Val Val Val Val Ser Thr Thr Gly Thr Gly Asp 50 55 60 Pro
Pro Asp Thr Ala Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr 65 70
75 80 Leu Pro Val Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly
Leu 85 90 95 Gly Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys
Ile Ile Asp 100 105 110 Lys Arg Leu Gln Glu Leu Gly Ala Arg His Phe
Tyr Asp Thr Gly His 115 120 125 Ala Asp Asp Cys Val Gly Leu Glu Leu
Val Val Glu Pro Trp Ile Ala 130 135 140 Gly Leu Trp Pro Ala Leu Arg
Lys His Phe Arg Ser Ser Arg Gly Gln 145 150 155 160 Glu Glu Ile Ser
Gly Ala Leu Pro Val Ala Ser Pro Ala Ser Leu Arg 165 170 175 Thr Asp
Leu Val Lys Ser Glu Leu Leu His Ile Glu Ser Gln Val Glu 180 185 190
Leu Leu Arg Phe Asp Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys 195
200 205 Gln Asn Ala Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp
Phe 210 215 220 Glu Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln
Ala Ser Leu 225 230 235 240 Asn Ile Pro Gly Leu Pro Pro Glu Tyr Leu
Gln Val His Leu Gln Glu 245 250 255 Ser Leu Gly Gln Glu Glu Ser Gln
Val Ser Val Thr Ser Ala Asp Pro 260 265 270 Val Phe Gln Val Pro Ile
Ser Lys Ala Val Gln Leu Thr Thr Asn Asp 275 280 285 Ala Ile Lys Thr
Thr Leu Leu Val Glu Leu Asp Ile Ser Asn Thr Asp 290 295 300 Phe Ser
Tyr Gln Pro Gly Asp Ala Phe Ser Val Ile Cys Pro Asn Ser 305 310 315
320 Asp Ser Glu Val Gln Ser Leu Leu Gln Arg Leu Gln Leu Glu Asp Lys
325 330 335 Arg Glu His Cys Val Leu Leu Lys Ile Lys Ala Asp Thr Lys
Lys Lys 340 345 350 Gly Ala Thr Leu Pro Gln His Ile Pro Ala Gly Cys
Ser Leu Gln Phe 355 360 365 Ile Phe Thr Trp Cys Leu Glu Ile Arg Ala
Ile Pro Lys Lys Ala Phe 370 375 380 Leu Arg Ala Leu Val Asp Tyr Thr
Ser Asp Ser Ala Glu Lys Arg Arg 385 390 395 400 Leu Gln Glu Leu Cys
Ser Lys Gln Gly Ala Ala Asp Tyr Ser Arg Phe 405 410 415 Val Arg Asp
Ala Cys Ala Cys Leu Leu Asp Leu Leu Leu Ala Phe Pro 420 425 430 Ser
Cys Gln Pro Pro Leu Ser Leu Leu Leu Glu His Leu Pro Lys Leu 435 440
445 Gln Pro Arg Pro Tyr Ser Cys Ala Ser Ser Ser Leu Phe His Pro Gly
450 455 460 Lys Leu His Phe Val Phe Asn Ile Val Glu Phe Leu Ser Thr
Ala Thr 465 470 475 480 Thr Glu Val Leu Arg Lys Gly Val Cys Thr Gly
Trp Leu Ala Leu Leu 485 490 495 Val Ala Ser Val Leu Gln Pro Asn Ile
His Ala Ser His Glu Asp Ser 500 505 510 Gly Lys Ala Leu Ala Pro Lys
Ile Ser Ile Ser Pro Arg Thr Thr Asn 515 520 525 Ser Phe His Leu Pro
Asp Asp Pro Ser Ile Pro Ile Ile Met Val Gly 530 535 540 Pro Gly Thr
Gly Ile Ala Pro Phe Ile Gly Phe Leu Gln His Arg Glu 545 550 555 560
Lys Leu Gln Glu Gln His Pro Asp Gly Asn Phe Gly Ala Met Trp Leu 565
570 575 Phe Phe Gly Cys Arg His Lys Asp Arg Asp Tyr Leu Phe Arg Lys
Glu 580 585 590 Leu Arg His Phe Leu Lys His Gly Ile Leu Thr His Leu
Lys Val Ser 595 600 605 Phe Ser Arg Asp Ala Pro Val Gly Glu Glu Glu
Ala Pro Ala Lys Tyr 610 615 620 Val Gln Asp Asn Ile Gln Leu His Gly
Gln Gln Val Ala Arg Ile Leu 625 630 635 640 Leu Gln Glu Asn Gly His
Ile Tyr Val Cys Gly Asp Ala Lys Asn Met 645 650 655 Ala Lys Asp Val
His Asp Ala Leu Val Gln Ile Ile Ser Lys Glu Val 660 665 670 Gly Val
Glu Lys Leu Glu Ala Met Lys Thr Leu Ala Thr Leu Lys Glu 675 680 685
Glu Lys Arg Tyr Leu Gln Asp Ile Trp Ser 690 695 3 24 DNA Homo
sapiens 3 ctcctgctcg aacatcttcc taaa 24 4 25 DNA Homo sapiens 4
aatagataat ccctatcctt atgcc 25 5 23 DNA Homo sapiens 5 ccctggctcc
taagatatcc atc 23 6 26 DNA Homo sapiens 6 cgaacaacaa attctttcca
cttacc 26 7 23 DNA Homo sapiens 7 caaggttggt ggaagtcgcg ttg 23 8 25
DNA Homo sapiens 8 atgccttgaa gtgatgagga ggttt 25 9 24 DNA Homo
sapiens 9 ttcctacaac atagagagaa actc 24 10 24 DNA Homo sapiens 10
ttgcacaagg gcatcatgta catc 24 11 25 DNA Homo sapiens 11 aaacctcctc
atcacttcaa ggcat 25 12 23 DNA Homo sapiens 12 cttgcacacg aatatggtct
ggg 23 13 23 DNA Homo sapiens 13 tggcatcacc tgcatccttg agg 23 14 25
DNA Homo sapiens 14 gatgtacctg taaatattct ggggg 25 15 24 DNA Homo
sapiens 15 aatccacggc tcaaccacaa gttc 24 16 25 DNA Homo sapiens 16
ctcgaaatta accctcacta aaggg 25 17 23 DNA Homo sapiens 17 aacccatacc
gcaggtgagc aaa 23 18 29 DNA Homo sapiens 18 tttagtactt tcagtcaaaa
aagcttaat 29 19 25 DNA Homo sapiens 19 ataaacgact tcaagagctt ggagc
25 20 25 DNA Homo sapiens 20 aggtttggca ctagtaaagc tgact 25 21 698
PRT Homo sapiens 21 Met Arg Arg Phe Leu Leu Leu Tyr Ala Thr Gln Gln
Gly Gln Ala Lys 1 5 10 15 Ala Ile Ala Glu Glu Met Cys Glu Gln Ala
Val Val His Gly Phe Ser 20 25 30 Ala Asp Leu His Cys Ile Ser Glu
Ser Asp Lys Tyr Asp Leu Lys Thr 35 40 45 Glu Thr Ala Pro Leu Val
Val Val Val Ser Thr Thr Gly Thr Gly Asp 50 55 60 Pro Pro Asp Thr
Ala Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr 65 70 75 80 Leu Pro
Val Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly Leu 85 90 95
Gly Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys Ile Ile Asp 100
105 110 Lys Arg Leu Gln Glu Leu Gly Ala Arg His Phe Tyr Asp Thr Gly
His 115 120 125 Ala Asp Asp Cys Val Gly Leu Glu Leu Val Val Glu Pro
Trp Ile Ala 130 135 140 Gly Leu Trp Pro Ala Leu Arg Lys His Phe Arg
Ser Ser Arg Gly Gln 145 150 155 160 Glu Glu Ile Ser Gly Ala Leu Pro
Val Ala Ser Pro Ala Ser Leu Arg 165 170 175 Thr Asp Leu Val Lys Ser
Glu Leu Leu His Ile Glu Ser Gln Val Glu 180 185 190 Leu Leu Arg Phe
Asp Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys 195 200 205 Gln Asn
Ala Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp Phe 210 215 220
Glu Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln Ala Ser Leu 225
230 235 240 Asn Ile Pro Gly Leu Pro Pro Glu Tyr Leu Gln Val His Leu
Gln Glu 245 250 255 Ser Leu Gly Gln Glu Glu Ser Gln Val Ser Val Thr
Ser Ala Asp Pro 260 265 270 Val Phe Gln Val Pro Ile Ser Lys Ala Val
Gln Leu Thr Thr Asn Asp 275 280 285 Ala Ile Lys Thr Thr Leu Leu Val
Glu Leu Asp Ile Ser Asn Thr Asp 290 295 300 Phe Ser Tyr Gln Pro Gly
Asp Ala Phe Ser Val Ile Cys Pro Asn Ser 305 310 315 320 Asp Ser Glu
Val Gln Ser Leu Leu Gln Arg Leu Gln Leu Glu Asp Lys 325 330 335 Arg
Glu His Cys Val Leu Leu Lys Ile Lys Ala Asp Thr Lys Lys Lys 340 345
350 Gly Ala Thr Leu Pro Gln His Ile Pro Ala Gly Cys Ser Leu Gln Phe
355 360 365 Ile Phe Thr Trp Cys Leu Glu Ile Arg Ala Ile Pro Lys Lys
Ala Phe 370 375 380 Leu Arg Ala Leu Val Asp Tyr Thr Ser Asp Ser Ala
Glu Lys Arg Arg 385 390 395 400 Leu Gln Glu Leu Cys Ser Lys Gln Gly
Ala Ala Asp Tyr Ser Arg Phe 405 410 415 Val Arg Asp Ala Cys Ala Cys
Leu Leu Asp Leu Leu Leu Ala Phe Pro 420 425 430 Ser Cys Gln Pro Pro
Leu Ser Leu Leu Leu Glu His Leu Pro Lys Leu 435 440 445 Gln Pro Arg
Pro Tyr Ser Cys Ala Ser Ser Ser Leu Phe His Pro Gly 450 455 460 Lys
Leu His Phe Val Phe Asn Ile Val Glu Phe Leu Ser Thr Ala Thr 465 470
475 480 Thr Glu Val Leu Arg Lys Gly Val Cys Thr Gly Trp Leu Ala Leu
Leu 485 490 495 Val Ala Ser Val Leu Gln Pro Asn Ile His Ala Ser His
Glu Asp Ser 500 505 510 Gly Lys Ala Leu Ala Pro Lys Ile Ser Ile Ser
Pro Arg Thr Thr Asn 515 520 525 Ser Phe His Leu Pro Asp Asp Pro Ser
Ile Pro Ile Ile Met Val Gly 530 535 540 Pro Gly Thr Gly Ile Ala Pro
Phe Ile Gly Phe Leu Gln His Arg Glu 545 550 555 560 Lys Leu Gln Glu
Gln His Pro Asp Gly Asn Phe Gly Ala Met Trp Leu 565 570 575 Phe Phe
Gly Cys Arg His Lys Asp Arg Asp Tyr Leu Phe Arg Lys Glu 580 585 590
Leu Arg His Phe Leu Lys His Gly Ile Leu Thr His Leu Lys Val Ser 595
600 605 Phe Ser Arg Asp Ala Pro Val Gly Glu Glu Glu Ala Pro Ala Lys
Tyr 610 615 620 Val Gln Asp Asn Ile Gln Leu His Gly Gln Gln Val Ala
Arg Ile Leu 625 630 635 640 Leu Gln Glu Asn Gly His Ile Tyr Val Cys
Gly Asp Ala Lys Asn Met 645 650 655 Ala Lys Asp Val His Asp Ala Leu
Val Gln Ile Ile Ser Lys Glu Val 660 665 670 Gly Val Glu Lys Leu Glu
Ala Met Lys Thr Leu Ala Thr Leu Lys Glu 675 680 685 Glu Lys Arg Tyr
Leu Gln Asp Ile Trp Ser 690 695 22 682 PRT Caenorhabditis elegans
22 Met Thr Asp Phe Leu Ile Ala Phe Gly Ser Gln Thr Gly Gln Ala Glu
1 5 10 15 Thr Ile Ala Lys Ser Leu Lys Glu Lys Ala Glu Leu Ile Gly
Leu Thr 20 25 30 Pro Arg Leu His Ala Leu Asp Glu Asn Glu Lys Lys
Phe Asn Leu Asn 35 40 45 Glu Glu Lys Leu Cys Ala Ile Val Val Ser
Ser Thr Gly Asp Gly Asp 50 55 60 Ala Pro Asp Asn Cys Ala Arg Phe
Val Arg Arg Ile Asn Arg Asn Ser 65 70 75 80 Leu Glu Asn Glu Tyr Leu
Lys Asn Leu Asp Tyr Val Leu Leu Gly Leu 85 90 95 Gly Asp Ser Asn
Tyr Ser Ser Tyr Gln Thr Ile Pro Arg Lys Ile Asp 100 105 110 Lys Gln
Leu Thr Ala Leu Gly Ala Asn Arg Leu Phe Asp Arg Ala Glu 115 120 125
Ala Asp Asp Gln Val Gly Leu Glu Leu Glu Val Glu Pro Trp Ile Glu 130
135 140 Lys Phe Phe Ala Thr Leu Ala Ser Arg Phe Asp Ile Ser Ala Asp
Lys 145 150 155 160 Met Asn Ala Ile Thr Glu Ser Ser Asn Leu Lys Leu
Asn Gln Val Lys 165 170 175 Thr Glu Glu Glu Lys Lys Ala Leu Leu Gln
Lys Arg Ile Glu Asp Glu 180 185 190 Glu Ser Asp Asp Glu Gly Arg Gly
Arg Val Ile Gly Ile Asp Met Leu 195 200 205 Ile Pro Glu His Tyr Asp
Tyr Pro Glu Ile Ser Leu Leu Lys Gly Ser 210 215 220 Gln Thr Leu Ser
Asn Asp Glu Asn Leu Arg Val Pro Ile Ala Pro Gln 225 230 235 240 Pro
Phe Ile Val Ser Ser Val Ser Asn Arg Lys Leu Pro Glu Asp Thr 245 250
255 Lys Leu Glu Trp Gln Asn Leu Cys Lys Met Pro Gly Val Val Thr Lys
260 265 270 Pro Phe Glu Val Leu Val Val Ser Ala Glu Phe Val Thr Asp
Pro Phe 275 280 285 Ser Lys Lys Ile Lys Thr Lys Arg Met Ile Thr Val
Asp Phe Gly Asp 290 295 300 His Ala Ala Glu Leu Gln Tyr Glu Pro Gly
Asp Ala Ile Tyr Phe Cys 305 310 315 320 Val Pro Asn Pro Ala Leu Glu
Val Asn Phe Ile Leu Lys Arg Cys Gly 325 330 335 Val Leu Asp Ile Ala
Asp Gln Gln Cys Glu Leu Ser Ile Asn Pro Lys 340 345 350 Thr Glu Lys
Ile Asn Ala Gln Ile Pro Gly His Val His Lys Ile Thr 355 360 365 Thr
Leu Arg His Met Phe Thr Thr Cys Leu Asp Ile Arg Arg Ala Pro 370 375
380 Gly Arg Pro Leu Ile Arg Val Leu Ala Glu Ser Thr Ser Asp Pro Asn
385 390 395 400 Glu Lys Arg Arg Leu Leu Glu Leu Cys Ser Ala Gln Gly
Met Lys Asp 405 410 415 Phe Thr Asp Phe Val Arg Thr Pro Gly Leu Ser
Leu Ala Asp Met Leu 420 425 430 Phe Ala Phe Pro Asn Val Lys Pro Pro
Val Asp Arg Leu Ile Glu Leu 435 440 445 Leu Pro Arg Leu Ile Pro Arg
Pro Tyr Ser Met Ser Ser Tyr Glu
Asn 450 455 460 Arg Lys Ala Arg Leu Ile Tyr Ser Glu Met Glu Phe Pro
Ala Thr Asp 465 470 475 480 Gly Arg Arg His Ser Arg Lys Gly Leu Ala
Thr Asp Trp Leu Asn Ser 485 490 495 Leu Arg Ile Gly Asp Lys Val Gln
Val Leu Gly Lys Glu Pro Ala Arg 500 505 510 Phe Arg Leu Pro Pro Leu
Gly Met Thr Lys Asn Ser Ala Gly Lys Leu 515 520 525 Pro Leu Leu Met
Val Gly Pro Gly Thr Gly Val Ser Val Phe Leu Ser 530 535 540 Phe Leu
His Phe Leu Arg Lys Leu Lys Gln Asp Ser Pro Ser Asp Phe 545 550 555
560 Val Asp Val Pro Arg Val Leu Phe Phe Gly Cys Arg Asp Ser Ser Val
565 570 575 Asp Ala Ile Tyr Met Ser Glu Leu Glu Met Phe Val Ser Glu
Gly Ile 580 585 590 Leu Thr Asp Leu Ile Ile Cys Glu Ser Glu Gln Lys
Gly Glu Arg Val 595 600 605 Gln Asp Gly Leu Arg Lys Tyr Leu Asp Lys
Val Leu Pro Phe Leu Thr 610 615 620 Ala Ser Thr Glu Ser Lys Ile Phe
Ile Cys Gly Asp Ala Lys Gly Met 625 630 635 640 Ser Lys Asp Val Trp
Gln Cys Phe Ser Asp Ile Val Ala Ser Asp Gln 645 650 655 Gly Ile Pro
Asp Leu Glu Ala Lys Lys Lys Leu Met Asp Leu Lys Lys 660 665 670 Ser
Asp Gln Tyr Ile Glu Asp Val Trp Gly 675 680 23 677 PRT Homo sapiens
23 Met Gly Asp Ser His Val Asp Thr Ser Ser Thr Val Ser Glu Ala Val
1 5 10 15 Ala Glu Glu Val Ser Leu Phe Ser Met Thr Asp Met Ile Leu
Phe Ser 20 25 30 Leu Ile Val Gly Leu Leu Thr Tyr Trp Phe Leu Phe
Arg Lys Lys Lys 35 40 45 Glu Glu Val Pro Glu Phe Thr Lys Ile Gln
Thr Leu Thr Ser Ser Val 50 55 60 Arg Glu Ser Ser Phe Val Glu Lys
Met Lys Lys Thr Gly Arg Asn Ile 65 70 75 80 Ile Val Phe Tyr Gly Ser
Gln Thr Gly Thr Ala Glu Glu Phe Ala Asn 85 90 95 Arg Leu Ser Lys
Asp Ala His Arg Tyr Gly Met Arg Gly Met Ser Ala 100 105 110 Asp Pro
Glu Glu Tyr Asp Leu Ala Asp Leu Ser Ser Leu Pro Glu Ile 115 120 125
Asp Asn Ala Leu Val Val Phe Cys Met Ala Thr Tyr Gly Glu Gly Asp 130
135 140 Pro Thr Asp Asn Ala Gln Asp Phe Tyr Asp Trp Leu Gln Glu Thr
Asp 145 150 155 160 Val Asp Leu Ser Gly Val Lys Phe Ala Val Phe Gly
Leu Gly Asn Lys 165 170 175 Thr Tyr Glu His Phe Asn Ala Met Gly Lys
Tyr Val Asp Lys Arg Leu 180 185 190 Glu Gln Leu Gly Ala Gln Arg Ile
Phe Glu Leu Gly Leu Gly Asp Asp 195 200 205 Asp Gly Asn Leu Glu Glu
Asp Phe Ile Thr Trp Arg Glu Gln Phe Trp 210 215 220 Pro Ala Val Cys
Glu His Phe Gly Val Glu Ala Thr Gly Glu Glu Ser 225 230 235 240 Ser
Ile Arg Gln Tyr Glu Leu Val Val His Thr Asp Ile Asp Ala Ala 245 250
255 Lys Val Tyr Met Gly Glu Met Gly Arg Leu Lys Ser Tyr Glu Asn Gln
260 265 270 Lys Pro Pro Phe Asp Ala Lys Asn Pro Phe Leu Ala Ala Val
Thr Thr 275 280 285 Asn Arg Lys Leu Asn Gln Gly Thr Glu Arg His Leu
Met His Leu Glu 290 295 300 Leu Asp Ile Ser Asp Ser Lys Ile Arg Tyr
Glu Ser Gly Asp His Val 305 310 315 320 Ala Val Tyr Pro Ala Asn Asp
Ser Ala Leu Val Asn Gln Leu Gly Lys 325 330 335 Ile Leu Gly Ala Asp
Leu Asp Val Val Met Ser Leu Asn Asn Leu Asp 340 345 350 Glu Glu Ser
Asn Lys Lys His Pro Phe Pro Cys Pro Thr Ser Tyr Arg 355 360 365 Thr
Ala Leu Thr Tyr Tyr Leu Asp Ile Thr Asn Pro Pro Arg Thr Asn 370 375
380 Val Leu Tyr Glu Leu Ala Gln Tyr Ala Ser Glu Pro Ser Glu Gln Glu
385 390 395 400 Leu Leu Arg Lys Met Ala Ser Ser Ser Gly Glu Gly Lys
Glu Leu Tyr 405 410 415 Leu Ser Trp Val Val Glu Ala Arg Arg His Ile
Leu Ala Ile Leu Gln 420 425 430 Asp Cys Pro Ser Leu Arg Pro Pro Ile
Asp His Leu Cys Glu Leu Leu 435 440 445 Pro Arg Leu Gln Ala Arg Tyr
Tyr Ser Ile Ala Ser Ser Ser Lys Val 450 455 460 His Pro Asn Ser Val
His Ile Cys Ala Val Val Val Glu Tyr Glu Thr 465 470 475 480 Lys Ala
Gly Arg Ile Asn Lys Gly Val Ala Thr Asn Trp Leu Arg Ala 485 490 495
Lys Glu Pro Val Gly Glu Asn Gly Gly Arg Ala Leu Val Pro Met Phe 500
505 510 Val Arg Lys Ser Gln Phe Arg Leu Pro Phe Lys Ala Thr Thr Pro
Val 515 520 525 Ile Met Val Gly Pro Gly Thr Gly Val Ala Pro Phe Ile
Gly Phe Ile 530 535 540 Gln Glu Arg Ala Trp Leu Arg Gln Gln Gly Lys
Glu Val Gly Glu Thr 545 550 555 560 Leu Leu Tyr Tyr Gly Cys Arg Arg
Ser Asp Glu Asp Tyr Leu Tyr Arg 565 570 575 Glu Glu Leu Ala Gln Phe
His Arg Asp Gly Ala Leu Thr Gln Leu Asn 580 585 590 Val Ala Phe Ser
Arg Glu Gln Ser His Lys Val Tyr Val Gln His Leu 595 600 605 Leu Lys
Gln Asp Arg Glu His Leu Trp Lys Leu Ile Glu Gly Gly Ala 610 615 620
His Ile Tyr Val Cys Gly Asp Ala Arg Asn Met Ala Arg Asp Val Gln 625
630 635 640 Asn Thr Phe Tyr Asp Ile Val Ala Glu Leu Gly Ala Met Glu
His Ala 645 650 655 Gln Ala Val Asp Tyr Ile Lys Lys Leu Met Thr Lys
Gly Arg Tyr Ser 660 665 670 Leu Asp Val Trp Ser 675 24 3259 DNA
Homo sapiens 24 caaggttggt ggaagtcgcg ttgtgcaggt tcgtgcccgg
ctggcgcggc gtggtttcac 60 tgttacatgc cttgaagtga tgaggaggtt
tctgttacta tatgctacac agcagggaca 120 ggcaaaggcc atcgcagaag
aaatgtgtga gcaagctgtg gtacatggat tttctgcaga 180 tcttcactgt
attagtgaat ccgataagta tgacctaaaa accgaaacag ctcctcttgt 240
tgttgtggtt tctaccacgg gcaccggaga cccacccgac acagcccgca agtttgttaa
300 ggaaatacag aaccaaacac tgccggttga tttctttgct cacctgcggt
atgggttact 360 gggtctcggt gattcagaat acacctactt ttgcaatggg
gggaagataa ttgataaacg 420 acttcaagag cttggagccc ggcatttcta
tgacactgga catgcagatg actgtgtagg 480 tttagaactt gtggttgagc
cgtggattgc tggactctgg ccagccctca gaaagcattt 540 taggtcaagc
agaggacaag aggagataag tggcgcactc ccggtggcat cacctgcatc 600
cttgaggaca gaccttgtga agtcagagct gctacacatt gaatctcaag tcgagcttct
660 gagattcgat gattcaggaa gaaaggattc tgaggttttg aagcaaaatg
cagtgaacag 720 caaccaatcc aatgttgtaa ttgaagactt tgagtcctca
cttacccgtt cggtaccccc 780 actctcacaa gcctctctga atattcctgg
tttaccccca gaatatttac aggtacatct 840 gcaggagtct cttggccagg
aggaaagcca agtatctgtg acttcagcag atccagtttt 900 tcaagtgcca
atttcaaagg cagttcaact tactacgaat gatgccataa aaaccactct 960
gctggtagaa ttggacattt caaatacaga cttttcctat cagcctggag atgccttcag
1020 cgtgatctgc cctaacagtg attctgaggt acaaagccta ctccaaagac
tgcagcttga 1080 agataaaaga gagcactgcg tccttttgaa aataaaggca
gacacaaaga agaaaggagc 1140 taccttaccc cagcatatac ctgcgggatg
ttctctccag ttcattttta cctggtgtct 1200 tgaaatccga gcaattccta
aaaaggcatt tttgcgagcc cttgtggact ataccagtga 1260 cagtgctgaa
aagcgcaggc tacaggagct gtgcagtaaa caaggggcag ccgattatag 1320
ccgctttgta cgagatgcct gtgcctgctt gttggatctc ctcctcgctt tcccttcttg
1380 ccagccacca ctcagtctcc tgctcgaaca tcttcctaaa cttcaaccca
gaccatattc 1440 gtgtgcaagc tcaagtttat ttcacccagg aaagctccat
tttgtcttca acattgtgga 1500 atttctgtct actgccacaa cagaggttct
gcggaaggga gtatgtacag gctggctggc 1560 cttgttggtt gcttcagttc
ttcagccaaa catacatgca tcccatgaag acagcgggaa 1620 agccctggct
cctaagatat ccatctctcc tcgaacaaca aattctttcc acttaccaga 1680
tgacccctca atccccatca taatggtggg tccaggaacc ggcatagccc cgtttattgg
1740 gttcctacaa catagagaga aactccaaga acaacaccca gatggaaatt
ttggagcaat 1800 gtggttgttt tttggctgca ggcataagga tagggattat
ctattcagaa aagagctcag 1860 acatttcctt aagcatggga tcttaactca
tctaaaggtt tccttctcaa gagatgctcc 1920 tgttggggag gaggaagccc
cagcaaagta tgtacaagac aacatccagc ttcatggcca 1980 gcaggtggcg
agaatcctcc tccaggagaa cggccatatt tatgtgtgtg gagatgcaaa 2040
gaatatggcc aaggatgtac atgatgccct tgtgcaaata ataagcaaag aggttggagt
2100 tgaaaaacta gaagcaatga aaaccctggc cactttaaaa gaagaaaaac
gctaccttca 2160 ggatatttgg tcataaaacc agaaattaaa gaaagaggat
taagcttttt tgactgaaag 2220 tactaaaagt cagctttact agtgccaaac
ctttaaattt tcaaaagaaa attttctttc 2280 aacatttctt gaaggacatg
gagtggagat tggatcattt aacaatataa caaaacttcc 2340 tgatttgatt
ttacgtatct tctatctacg cccttcctgt gcctgtgact ctccccaaat 2400
tgccctgttg ccttgagctc ttctgagcta aaggcagcct tcagtcccta tcagcgcctc
2460 ctttacttcc cagagaactt cacagagact ctgtccttcc atgcaaaggc
ttcctgaaat 2520 aggggagact gactgagtag ctcattcttg tgacttacag
tgccaacatt taaaaaagta 2580 tgaaaatgat ttatttttat atgatgtata
cccataaaga atgctcatat taatgtactt 2640 aaattacaca tgtagagcat
atctgttata tgtttatgta actatcaaat ggttatttgt 2700 tactaaagct
atatttctga taaaaaatat tttaggataa ttgcctacag agggatttat 2760
ttttatgatg ctgggaaata tgaaatgtat tttaaaattt cactctgggc atatggattt
2820 atctatcacc attacttttt tttaagtcac aatttcagaa ttttgggaca
tttgcattca 2880 atttacaggt accagtacgt acatatttta atagaaagat
acaacctttt tattttcact 2940 ccttttattt ctgctgcttg gcacattttt
gagttttccc acattatttg tctccatgat 3000 accactcaag cagtgtgctg
gacctaaaat actgacttta gttagtatcc ttggattttt 3060 agattcccca
gtgtctaatt ccctgttata atttgcacaa acaaaacaaa atgttatgat 3120
aatctttctc cactgttcta atatatattg tatttttatt tgatagcttg ggatttaaaa
3180 catctctgtt gaaggctttt gatccttttg agaaataaag atctgaaaga
aatggcataa 3240 tcttaaaaaa aaaaaaaaa 3259 25 18 PRT Homo sapiens 25
Gly Ala Met Trp Leu Phe Phe Gly Cys Arg His Lys Asp Arg Asp Tyr 1 5
10 15 Leu Phe 26 18 PRT Homo sapiens 26 Gly Glu Thr Leu Leu Tyr Tyr
Gly Cys Arg Arg Ser Asp Glu Asp Tyr 1 5 10 15 Leu Tyr 27 18 PRT
Oryctolagus cuniculus 27 Gly Glu Thr Leu Leu Tyr Tyr Gly Cys Arg
Arg Ala Ala Glu Asp Tyr 1 5 10 15 Leu Tyr 28 18 PRT Drosophila
melanogaster 28 Gly Glu Ser Ile Leu Tyr Phe Gly Cys Arg Lys Arg Ser
Glu Asp Tyr 1 5 10 15 Ile Tyr 29 18 PRT Vigna radiata 29 Gly Pro
Ala Leu Leu Phe Phe Gly Cys Arg Asn Arg Gln Met Asp Phe 1 5 10 15
Ile Tyr 30 18 PRT Aspergillus niger 30 Gly Pro Thr Val Leu Phe Phe
Gly Cys Arg Lys Ser Asp Glu Asp Phe 1 5 10 15 Leu Tyr 31 18 PRT
Homo sapiens 31 Cys Pro Met Val Leu Val Phe Gly Cys Arg Gln Ser Lys
Ile Asp His 1 5 10 15 Ile Tyr 32 18 PRT Homo sapiens 32 Gly Arg Met
Thr Leu Val Phe Gly Cys Arg Arg Pro Asp Glu Asp His 1 5 10 15 Ile
Tyr 33 18 PRT Homo sapiens 33 Thr Pro Met Thr Leu Val Phe Gly Cys
Arg Cys Ser Gln Leu Asp His 1 5 10 15 Leu Tyr 34 18 PRT Oryctolagus
cuniculus 34 Gly Arg Met Thr Leu Val Phe Gly Cys Arg His Pro Glu
Glu Asp His 1 5 10 15 Leu Tyr 35 18 PRT Gallus gallus 35 Gly Asp
Met Ile Leu Leu Phe Gly Cys Arg His Pro Asp Met Asp His 1 5 10 15
Ile Tyr 36 18 PRT Escherichia coli 36 Gly Lys Asn Trp Leu Phe Phe
Gly Asn Pro His Phe Thr Glu Asp Phe 1 5 10 15 Leu Tyr 37 18 PRT
Saccharomyces cerevisiae 37 Gly Glu Val Phe Leu Tyr Leu Gly Ser Arg
His Lys Arg Glu Glu Tyr 1 5 10 15 Leu Tyr 38 18 PRT Thiocapsa
roseopersicina 38 Gly Arg Asn Trp Leu Ile Phe Gly Asn Arg His Phe
His Arg Asp Phe 1 5 10 15 Leu Tyr 39 19 PRT Pisum sativum 39 Gly
Leu Ala Trp Leu Phe Leu Gly Val Ala Asn Val Asp Ser Leu Leu 1 5 10
15 Tyr Asp Asp 40 18 PRT Spinacia oleracea 40 Gly Leu Ala Trp Leu
Phe Leu Gly Val Pro Thr Ser Ser Ser Leu Leu 1 5 10 15 Tyr Lys 41
2097 DNA Homo sapiens 41 atgaggaggt ttctgttact atatgctaca
cagcagggac aggcaaaggc catcgcagaa 60 gaaatatgtg agcaagctgt
ggtacatgga ttttctgcag atcttcactg tattagtgaa 120 tccgataagt
atgacctaaa aaccgaaaca gctcctcttg ttgttgtggt ttctaccacg 180
ggcaccggag acccacccga cacagcccgc aagtttgtta aggaaataca gaaccaaaca
240 ctgccggttg atttctttgc tcacctgcgg tatgggttac tgggtctcgg
tgattcagaa 300 tacacctact tttgcaatgg ggggaagata attgataaac
gacttcaaga gcttggagcc 360 cggcatttct atgacactgg acatgcagat
gactgtgtag gtttagaact tgtggttgag 420 ccgtggattg ctggactctg
gccagccctc agaaagcatt ttaggtcaag cagaggacaa 480 gaggagataa
gtggcgcact cccggtggca tcacctgcat ccttgaggac agaccttgtg 540
aagtcagagc tgctacacat tgaatctcaa gtcgagcttc tgagattcga tgattcagga
600 agaaaggatt ctgaggtttt gaagcaaaat gcagtgaaca gcaaccaatc
caatgttgta 660 attgaagact ttgagtcctc acttacccgt tcggtacccc
cactctcaca agcctctctg 720 aatattcctg gtttaccccc agaatattta
caggtacatc tgcaggagtc tcttggccag 780 gaggaaagcc aagtatctgt
gacttcagca gatccagttt ttcaagtgcc aatttcaaag 840 gcagttcaac
ttactacgaa tgatgccata aaaaccactc tgctggtaga attggacatt 900
tcaaatacag acttttccta tcagcctgga gatgccttca gcgtgatctg ccctaacagt
960 gattctgagg tacaaagcct actccaaaga ctgcagcttg aagataaaag
agagcactgc 1020 gtccttttga aaataaaggc agacacaaag aagaaaggag
ctaccttacc ccagcatata 1080 cctgcgggat gttctctcca gttcattttt
acctggtgtc ttgaaatccg agcaattcct 1140 aaaaaggcat ttttgcgagc
ccttgtggac tataccagtg acagtgctga aaagcgcagg 1200 ctacaggagc
tgtgcagtaa acaaggggca gccgattata gccgctttgt acgagatgcc 1260
tgtgcctgct tgttggatct cctcctcgct ttcccttctt gccagccacc actcagtctc
1320 ctgctcgaac atcttcctaa acttcaaccc agaccatatt cgtgtgcaag
ctcaagttta 1380 tttcacccag gaaagctcca ttttgtcttc aacattgtgg
aatttctgtc tactgccaca 1440 acagaggttc tgcggaaggg agtatgtaca
ggctggctgg ccttgttggt tgcttcagtt 1500 cttcagccaa acatacatgc
atcccatgaa gacagcggga aagccctggc tcctaagata 1560 tccatctctc
ctcgaacaac aaattctttc cacttaccag atgacccctc aatccccatc 1620
ataatggtgg gtccaggaac cggcatagcc ccgtttattg ggttcctaca acatagagag
1680 aaactccaag aacaacaccc agatggaaat tttggagcaa tgtggttgtt
ttttggctgc 1740 aggcataagg atagggatta tctattcaga aaagagctca
gacatttcct taagcatggg 1800 atcttaactc atctaaaggt ttccttctca
agagatgctc ctgttgggga ggaggaagcc 1860 ccagcaaagt atgtacaaga
caacatccag cttcatggcc agcaggtggc gagaatcctc 1920 ctccaggaga
acggccatat ttatgtgtgt ggagatgcaa agaatatggc caaggatgta 1980
catgatgccc ttgtgcaaat aataagcaaa gaggttggag ttgaaaaact agaagcaatg
2040 aaaaccctgg ccactttaaa agaagaaaaa cgctaccttc aggatatttg gtcataa
2097 42 698 PRT Homo sapiens 42 Met Arg Arg Phe Leu Leu Leu Tyr Ala
Thr Gln Gln Gly Gln Ala Lys 1 5 10 15 Ala Ile Ala Glu Glu Ile Cys
Glu Gln Ala Val Val His Gly Phe Ser 20 25 30 Ala Asp Leu His Cys
Ile Ser Glu Ser Asp Lys Tyr Asp Leu Lys Thr 35 40 45 Glu Thr Ala
Pro Leu Val Val Val Val Ser Thr Thr Gly Thr Gly Asp 50 55 60 Pro
Pro Asp Thr Ala Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr 65 70
75 80 Leu Pro Val Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly
Leu 85 90 95 Gly Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys
Ile Ile Asp 100 105 110 Lys Arg Leu Gln Glu Leu Gly Ala Arg His Phe
Tyr Asp Thr Gly His 115 120 125 Ala Asp Asp Cys Val Gly Leu Glu Leu
Val Val Glu Pro Trp Ile Ala 130 135 140 Gly Leu Trp Pro Ala Leu Arg
Lys His Phe Arg Ser Ser Arg Gly Gln 145 150 155 160 Glu Glu Ile Ser
Gly Ala Leu Pro Val Ala Ser Pro Ala Ser Leu Arg 165 170 175 Thr Asp
Leu Val Lys Ser Glu Leu Leu His Ile Glu Ser Gln Val Glu 180 185 190
Leu Leu Arg Phe Asp Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys 195
200 205 Gln Asn Ala Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp
Phe 210 215 220 Glu Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln
Ala Ser Leu 225 230 235 240 Asn Ile Pro Gly Leu Pro Pro Glu Tyr Leu
Gln Val His Leu Gln Glu 245 250 255 Ser Leu Gly Gln Glu Glu Ser Gln
Val Ser Val Thr Ser Ala Asp Pro 260 265 270 Val Phe Gln Val Pro Ile
Ser Lys Ala Val Gln Leu Thr Thr Asn Asp 275 280 285 Ala Ile Lys Thr
Thr Leu Leu Val Glu Leu Asp Ile Ser Asn Thr Asp 290
295 300 Phe Ser Tyr Gln Pro Gly Asp Ala Phe Ser Val Ile Cys Pro Asn
Ser 305 310 315 320 Asp Ser Glu Val Gln Ser Leu Leu Gln Arg Leu Gln
Leu Glu Asp Lys 325 330 335 Arg Glu His Cys Val Leu Leu Lys Ile Lys
Ala Asp Thr Lys Lys Lys 340 345 350 Gly Ala Thr Leu Pro Gln His Ile
Pro Ala Gly Cys Ser Leu Gln Phe 355 360 365 Ile Phe Thr Trp Cys Leu
Glu Ile Arg Ala Ile Pro Lys Lys Ala Phe 370 375 380 Leu Arg Ala Leu
Val Asp Tyr Thr Ser Asp Ser Ala Glu Lys Arg Arg 385 390 395 400 Leu
Gln Glu Leu Cys Ser Lys Gln Gly Ala Ala Asp Tyr Ser Arg Phe 405 410
415 Val Arg Asp Ala Cys Ala Cys Leu Leu Asp Leu Leu Leu Ala Phe Pro
420 425 430 Ser Cys Gln Pro Pro Leu Ser Leu Leu Leu Glu His Leu Pro
Lys Leu 435 440 445 Gln Pro Arg Pro Tyr Ser Cys Ala Ser Ser Ser Leu
Phe His Pro Gly 450 455 460 Lys Leu His Phe Val Phe Asn Ile Val Glu
Phe Leu Ser Thr Ala Thr 465 470 475 480 Thr Glu Val Leu Arg Lys Gly
Val Cys Thr Gly Trp Leu Ala Leu Leu 485 490 495 Val Ala Ser Val Leu
Gln Pro Asn Ile His Ala Ser His Glu Asp Ser 500 505 510 Gly Lys Ala
Leu Ala Pro Lys Ile Ser Ile Ser Pro Arg Thr Thr Asn 515 520 525 Ser
Phe His Leu Pro Asp Asp Pro Ser Ile Pro Ile Ile Met Val Gly 530 535
540 Pro Gly Thr Gly Ile Ala Pro Phe Ile Gly Phe Leu Gln His Arg Glu
545 550 555 560 Lys Leu Gln Glu Gln His Pro Asp Gly Asn Phe Gly Ala
Met Trp Leu 565 570 575 Phe Phe Gly Cys Arg His Lys Asp Arg Asp Tyr
Leu Phe Arg Lys Glu 580 585 590 Leu Arg His Phe Leu Lys His Gly Ile
Leu Thr His Leu Lys Val Ser 595 600 605 Phe Ser Arg Asp Ala Pro Val
Gly Glu Glu Glu Ala Pro Ala Lys Tyr 610 615 620 Val Gln Asp Asn Ile
Gln Leu His Gly Gln Gln Val Ala Arg Ile Leu 625 630 635 640 Leu Gln
Glu Asn Gly His Ile Tyr Val Cys Gly Asp Ala Lys Asn Met 645 650 655
Ala Lys Asp Val His Asp Ala Leu Val Gln Ile Ile Ser Lys Glu Val 660
665 670 Gly Val Glu Lys Leu Glu Ala Met Lys Thr Leu Ala Thr Leu Lys
Glu 675 680 685 Glu Lys Arg Tyr Leu Gln Asp Ile Trp Ser 690 695 43
2097 DNA Homo sapiens 43 atgaggaggt ttctgttact atatgctaca
cagcagggac aggcaaaggc catcgcagaa 60 gaaatgtgtg agcaagctgt
ggtacatgga ttttctgcag atcttcacta tattagtgaa 120 tccgataagt
atgacctaaa aaccgaaaca gctcctcttg ttgttgtggt ttctaccacg 180
ggcaccggag acccacccga cacagcccgc aagtttgtta aggaaataca gaaccaaaca
240 ctgccggttg atttctttgc tcacctgcgg tatgggttac tgggtctcgg
tgattcagaa 300 tacacctact tttgcaatgg ggggaagata attgataaac
gacttcaaga gcttggagcc 360 cggcatttct atgacactgg acatgcagat
gactgtgtag gtttagaact tgtggttgag 420 ccgtggattg ctggactctg
gccagccctc agaaagcatt ttaggtcaag cagaggacaa 480 gaggagataa
gtggcgcact cccggtggca tcacctgcat ccttgaggac agaccttgtg 540
aagtcagagc tgctacacat tgaatctcaa gtcgagcttc tgagattcga tgattcagga
600 agaaaggatt ctgaggtttt gaagcaaaat gcagtgaaca gcaaccaatc
caatgttgta 660 attgaagact ttgagtcctc acttacccgt tcggtacccc
cactctcaca agcctctctg 720 aatattcctg gtttaccccc agaatattta
caggtacatc tgcaggagtc tcttggccag 780 gaggaaagcc aagtatctgt
gacttcagca gatccagttt ttcaagtgcc aatttcaaag 840 gcagttcaac
ttactacgaa tgatgccata aaaaccactc tgctggtaga attggacatt 900
tcaaatacag acttttccta tcagcctgga gatgccttca gcgtgatctg ccctaacagt
960 gattctgagg tacaaagcct actccaaaga ctgcagcttg aagataaaag
agagcactgc 1020 gtccttttga aaataaaggc agacacaaag aagaaaggag
ctaccttacc ccagcatata 1080 cctgcgggat gttctctcca gttcattttt
acctggtgtc ttgaaatccg agcaattcct 1140 aaaaaggcat ttttgcgagc
ccttgtggac tataccagtg acagtgctga aaagcgcagg 1200 ctacaggagc
tgtgcagtaa acaaggggca gccgattata gccgctttgt acgagatgcc 1260
tgtgcctgct tgttggatct cctcctcgct ttcccttctt gccagccacc actcagtctc
1320 ctgctcgaac atcttcctaa acttcaaccc agaccatatt cgtgtgcaag
ctcaagttta 1380 tttcacccag gaaagctcca ttttgtcttc aacattgtgg
aatttctgtc tactgccaca 1440 acagaggttc tgcggaaggg agtatgtaca
ggctggctgg ccttgttggt tgcttcagtt 1500 cttcagccaa acatacatgc
atcccatgaa gacagcggga aagccctggc tcctaagata 1560 tccatctctc
ctcgaacaac aaattctttc cacttaccag atgacccctc aatccccatc 1620
ataatggtgg gtccaggaac cggcatagcc ccgtttattg ggttcctaca acatagagag
1680 aaactccaag aacaacaccc agatggaaat tttggagcaa tgtggttgtt
ttttggctgc 1740 aggcataagg atagggatta tctattcaga aaagagctca
gacatttcct taagcatggg 1800 atcttaactc atctaaaggt ttccttctca
agagatgctc ctgttgggga ggaggaagcc 1860 ccagcaaagt atgtacaaga
caacatccag cttcatggcc agcaggtggc gagaatcctc 1920 ctccaggaga
acggccatat ttatgtgtgt ggagatgcaa agaatatggc caaggatgta 1980
catgatgccc ttgtgcaaat aataagcaaa gaggttggag ttgaaaaact agaagcaatg
2040 aaaaccctgg ccactttaaa agaagaaaaa cgctaccttc aggatatttg gtcataa
2097 44 698 PRT Homo sapiens 44 Met Arg Arg Phe Leu Leu Leu Tyr Ala
Thr Gln Gln Gly Gln Ala Lys 1 5 10 15 Ala Ile Ala Glu Glu Met Cys
Glu Gln Ala Val Val His Gly Phe Ser 20 25 30 Ala Asp Leu His Thr
Ile Ser Glu Ser Asp Lys Tyr Asp Leu Lys Thr 35 40 45 Glu Thr Ala
Pro Leu Val Val Val Val Ser Thr Thr Gly Thr Gly Asp 50 55 60 Pro
Pro Asp Thr Ala Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr 65 70
75 80 Leu Pro Val Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly
Leu 85 90 95 Gly Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys
Ile Ile Asp 100 105 110 Lys Arg Leu Gln Glu Leu Gly Ala Arg His Phe
Tyr Asp Thr Gly His 115 120 125 Ala Asp Asp Cys Val Gly Leu Glu Leu
Val Val Glu Pro Trp Ile Ala 130 135 140 Gly Leu Trp Pro Ala Leu Arg
Lys His Phe Arg Ser Ser Arg Gly Gln 145 150 155 160 Glu Glu Ile Ser
Gly Ala Leu Pro Val Ala Ser Pro Ala Ser Leu Arg 165 170 175 Thr Asp
Leu Val Lys Ser Glu Leu Leu His Ile Glu Ser Gln Val Glu 180 185 190
Leu Leu Arg Phe Asp Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys 195
200 205 Gln Asn Ala Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp
Phe 210 215 220 Glu Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln
Ala Ser Leu 225 230 235 240 Asn Ile Pro Gly Leu Pro Pro Glu Tyr Leu
Gln Val His Leu Gln Glu 245 250 255 Ser Leu Gly Gln Glu Glu Ser Gln
Val Ser Val Thr Ser Ala Asp Pro 260 265 270 Val Phe Gln Val Pro Ile
Ser Lys Ala Val Gln Leu Thr Thr Asn Asp 275 280 285 Ala Ile Lys Thr
Thr Leu Leu Val Glu Leu Asp Ile Ser Asn Thr Asp 290 295 300 Phe Ser
Tyr Gln Pro Gly Asp Ala Phe Ser Val Ile Cys Pro Asn Ser 305 310 315
320 Asp Ser Glu Val Gln Ser Leu Leu Gln Arg Leu Gln Leu Glu Asp Lys
325 330 335 Arg Glu His Cys Val Leu Leu Lys Ile Lys Ala Asp Thr Lys
Lys Lys 340 345 350 Gly Ala Thr Leu Pro Gln His Ile Pro Ala Gly Cys
Ser Leu Gln Phe 355 360 365 Ile Phe Thr Trp Cys Leu Glu Ile Arg Ala
Ile Pro Lys Lys Ala Phe 370 375 380 Leu Arg Ala Leu Val Asp Tyr Thr
Ser Asp Ser Ala Glu Lys Arg Arg 385 390 395 400 Leu Gln Glu Leu Cys
Ser Lys Gln Gly Ala Ala Asp Tyr Ser Arg Phe 405 410 415 Val Arg Asp
Ala Cys Ala Cys Leu Leu Asp Leu Leu Leu Ala Phe Pro 420 425 430 Ser
Cys Gln Pro Pro Leu Ser Leu Leu Leu Glu His Leu Pro Lys Leu 435 440
445 Gln Pro Arg Pro Tyr Ser Cys Ala Ser Ser Ser Leu Phe His Pro Gly
450 455 460 Lys Leu His Phe Val Phe Asn Ile Val Glu Phe Leu Ser Thr
Ala Thr 465 470 475 480 Thr Glu Val Leu Arg Lys Gly Val Cys Thr Gly
Trp Leu Ala Leu Leu 485 490 495 Val Ala Ser Val Leu Gln Pro Asn Ile
His Ala Ser His Glu Asp Ser 500 505 510 Gly Lys Ala Leu Ala Pro Lys
Ile Ser Ile Ser Pro Arg Thr Thr Asn 515 520 525 Ser Phe His Leu Pro
Asp Asp Pro Ser Ile Pro Ile Ile Met Val Gly 530 535 540 Pro Gly Thr
Gly Ile Ala Pro Phe Ile Gly Phe Leu Gln His Arg Glu 545 550 555 560
Lys Leu Gln Glu Gln His Pro Asp Gly Asn Phe Gly Ala Met Trp Leu 565
570 575 Phe Phe Gly Cys Arg His Lys Asp Arg Asp Tyr Leu Phe Arg Lys
Glu 580 585 590 Leu Arg His Phe Leu Lys His Gly Ile Leu Thr His Leu
Lys Val Ser 595 600 605 Phe Ser Arg Asp Ala Pro Val Gly Glu Glu Glu
Ala Pro Ala Lys Tyr 610 615 620 Val Gln Asp Asn Ile Gln Leu His Gly
Gln Gln Val Ala Arg Ile Leu 625 630 635 640 Leu Gln Glu Asn Gly His
Ile Tyr Val Cys Gly Asp Ala Lys Asn Met 645 650 655 Ala Lys Asp Val
His Asp Ala Leu Val Gln Ile Ile Ser Lys Glu Val 660 665 670 Gly Val
Glu Lys Leu Glu Ala Met Lys Thr Leu Ala Thr Leu Lys Glu 675 680 685
Glu Lys Arg Tyr Leu Gln Asp Ile Trp Ser 690 695 45 2094 DNA Homo
sapiens 45 atgaggaggt ttctgttact atatgctaca cagcagggac aggcaaaggc
catcgcagaa 60 gaaatgtgtg agcaagctgt ggtacatgga ttttctgcag
atcttcactg tattagtgaa 120 tccgataagt atgacctaaa aaccgaaaca
gctcctcttg ttgttgtggt ttctaccacg 180 ggcaccggag acccacccga
cacagcccgc aagtttgtta aggaaataca gaaccaaaca 240 ctgccggttg
atttctttgc tcacctgcgg tatgggttac tgggtctcgg tgattcagaa 300
tacacctact tttgcaatgg ggggaagata attgataaac gacttcaaga gcttggagcc
360 cggcatttct atgacactgg acatgcagat gactgtgtag gtttagaact
tgtggttgag 420 ccgtggattg ctggactctg gccagccctc agaaagcatt
ttaggtcaag cagaggacaa 480 gaggagataa gtggcgcact cccggtggca
tcacctgcat ccttgaggac agaccttgtg 540 aagtcagagc tgctacacat
tgaatctcaa gtcgagcttc tgagattcga tgattcagga 600 agaaaggatt
ctgaggtttt gaagcaaaat gcagtgaaca gcaaccaatc caatgttgta 660
attgaagact ttgagtcctc acttacccgt tcggtacccc cactctcaca agcctctctg
720 aatattcctg gtttaccccc agaatattta caggtacatc tgcaggagtc
tcttggccag 780 gaggaaagcc aagtatctgt gacttcagca gatccagttt
ttcaagtgcc aatttcaaag 840 gcagttcaac ttactacgaa tgatgccata
aaaaccactc tgctggtaga attggacatt 900 tcaaatacag acttttccta
tcagcctgga gatgccttca gcgtgatctg ccctaacagt 960 gattctgagg
tacaaagcct actccaaaga ctgcagcttg aagataaaag agagcactgc 1020
gtccttttga aaataaaggc agacacaaag aagaaaggag ctaccttacc ccagcatata
1080 cctgcgggat gttctctcca gttcattttt acctggtgtc ttgaaatccg
agcaattcct 1140 aaaaaggcat ttttgcgagc ccttgtggac tataccagtg
acagtgctga aaagcgcagg 1200 ctacaggagc tgtgcagtaa acaaggggca
gccgattata gccgctttgt acgagatgcc 1260 tgtgcctgct tgttggatct
cctcctcgct ttcccttctt gccagccacc actcagtctc 1320 ctgctcgaac
atcttcctaa acttcaaccc agaccatatt cgtgtgcaag ctcaagttta 1380
tttcacccag gaaagctcca ttttgtcttc aacattgtgg aatttctgtc tactgccaca
1440 acagaggttc tgcggaaggg agtatgtaca ggctggctgg ccttgttggt
tgcttcagtt 1500 cttcagccaa acatacatgc atcccatgaa gacagcggga
aagccctggc tcctaagata 1560 tccatctctc ctcgaacaac aaattctttc
cacttaccag atgacccctc aatccccatc 1620 ataatggtgg gtccaggaac
cggcatagcc ccgtttattg ggttcctaca acatagagag 1680 aaactccaag
aacaacaccc agatggaaat tttggagcaa tgtggttttt tggctgcagg 1740
cataaggata gggattatct attcagaaaa gagctcagac atttccttaa gcatgggatc
1800 ttaactcatc taaaggtttc cttctcaaga gatgctcctg ttggggagga
ggaagcccca 1860 gcaaagtatg tacaagacaa catccagctt catggccagc
aggtggcgag aatcctcctc 1920 caggagaacg gccatattta tgtgtgtgga
gatgcaaaga atatggccaa ggatgtacat 1980 gatgcccttg tgcaaataat
aagcaaagag gttggagttg aaaaactaga agcaatgaaa 2040 accctggcca
ctttaaaaga agaaaaacgc taccttcagg atatttggtc ataa 2094 46 697 PRT
Homo sapiens 46 Met Arg Arg Phe Leu Leu Leu Tyr Ala Thr Gln Gln Gly
Gln Ala Lys 1 5 10 15 Ala Ile Ala Glu Glu Met Cys Glu Gln Ala Val
Val His Gly Phe Ser 20 25 30 Ala Asp Leu His Cys Ile Ser Glu Ser
Asp Lys Tyr Asp Leu Lys Thr 35 40 45 Glu Thr Ala Pro Leu Val Val
Val Val Ser Thr Thr Gly Thr Gly Asp 50 55 60 Pro Pro Asp Thr Ala
Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr 65 70 75 80 Leu Pro Val
Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly Leu 85 90 95 Gly
Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys Ile Ile Asp 100 105
110 Lys Arg Leu Gln Glu Leu Gly Ala Arg His Phe Tyr Asp Thr Gly His
115 120 125 Ala Asp Asp Cys Val Gly Leu Glu Leu Val Val Glu Pro Trp
Ile Ala 130 135 140 Gly Leu Trp Pro Ala Leu Arg Lys His Phe Arg Ser
Ser Arg Gly Gln 145 150 155 160 Glu Glu Ile Ser Gly Ala Leu Pro Val
Ala Ser Pro Ala Ser Leu Arg 165 170 175 Thr Asp Leu Val Lys Ser Glu
Leu Leu His Ile Glu Ser Gln Val Glu 180 185 190 Leu Leu Arg Phe Asp
Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys 195 200 205 Gln Asn Ala
Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp Phe 210 215 220 Glu
Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln Ala Ser Leu 225 230
235 240 Asn Ile Pro Gly Leu Pro Pro Glu Tyr Leu Gln Val His Leu Gln
Glu 245 250 255 Ser Leu Gly Gln Glu Glu Ser Gln Val Ser Val Thr Ser
Ala Asp Pro 260 265 270 Val Phe Gln Val Pro Ile Ser Lys Ala Val Gln
Leu Thr Thr Asn Asp 275 280 285 Ala Ile Lys Thr Thr Leu Leu Val Glu
Leu Asp Ile Ser Asn Thr Asp 290 295 300 Phe Ser Tyr Gln Pro Gly Asp
Ala Phe Ser Val Ile Cys Pro Asn Ser 305 310 315 320 Asp Ser Glu Val
Gln Ser Leu Leu Gln Arg Leu Gln Leu Glu Asp Lys 325 330 335 Arg Glu
His Cys Val Leu Leu Lys Ile Lys Ala Asp Thr Lys Lys Lys 340 345 350
Gly Ala Thr Leu Pro Gln His Ile Pro Ala Gly Cys Ser Leu Gln Phe 355
360 365 Ile Phe Thr Trp Cys Leu Glu Ile Arg Ala Ile Pro Lys Lys Ala
Phe 370 375 380 Leu Arg Ala Leu Val Asp Tyr Thr Ser Asp Ser Ala Glu
Lys Arg Arg 385 390 395 400 Leu Gln Glu Leu Cys Ser Lys Gln Gly Ala
Ala Asp Tyr Ser Arg Phe 405 410 415 Val Arg Asp Ala Cys Ala Cys Leu
Leu Asp Leu Leu Leu Ala Phe Pro 420 425 430 Ser Cys Gln Pro Pro Leu
Ser Leu Leu Leu Glu His Leu Pro Lys Leu 435 440 445 Gln Pro Arg Pro
Tyr Ser Cys Ala Ser Ser Ser Leu Phe His Pro Gly 450 455 460 Lys Leu
His Phe Val Phe Asn Ile Val Glu Phe Leu Ser Thr Ala Thr 465 470 475
480 Thr Glu Val Leu Arg Lys Gly Val Cys Thr Gly Trp Leu Ala Leu Leu
485 490 495 Val Ala Ser Val Leu Gln Pro Asn Ile His Ala Ser His Glu
Asp Ser 500 505 510 Gly Lys Ala Leu Ala Pro Lys Ile Ser Ile Ser Pro
Arg Thr Thr Asn 515 520 525 Ser Phe His Leu Pro Asp Asp Pro Ser Ile
Pro Ile Ile Met Val Gly 530 535 540 Pro Gly Thr Gly Ile Ala Pro Phe
Ile Gly Phe Leu Gln His Arg Glu 545 550 555 560 Lys Leu Gln Glu Gln
His Pro Asp Gly Asn Phe Gly Ala Met Trp Phe 565 570 575 Phe Gly Cys
Arg His Lys Asp Arg Asp Tyr Leu Phe Arg Lys Glu Leu 580 585 590 Arg
His Phe Leu Lys His Gly Ile Leu Thr His Leu Lys Val Ser Phe 595 600
605 Ser Arg Asp Ala Pro Val Gly Glu Glu Glu Ala Pro Ala Lys Tyr Val
610 615 620 Gln Asp Asn Ile Gln Leu His Gly Gln Gln Val Ala Arg Ile
Leu Leu 625 630 635 640 Gln Glu Asn Gly His Ile Tyr Val Cys Gly Asp
Ala Lys Asn Met Ala 645 650 655 Lys
Asp Val His Asp Ala Leu Val Gln Ile Ile Ser Lys Glu Val Gly 660 665
670 Val Glu Lys Leu Glu Ala Met Lys Thr Leu Ala Thr Leu Lys Glu Glu
675 680 685 Lys Arg Tyr Leu Gln Asp Ile Trp Ser 690 695 47 2093 DNA
Homo sapiens 47 atgaggaggt ttctgttact atatgctaca cagcagggac
aggcaaaggc catcgcagaa 60 gaaatgtgtg agcaagctgt ggtacatgga
ttttctgcag atcttcactg tattagtgaa 120 tccgataagt atgacctaaa
aaccgaaaca gctcctcttg ttgttgtggt ttctaccacg 180 ggcaccggag
acccacccga cacagcccgc aagtttgtta aggaaataca gaaccaaaca 240
ctgccggttg atttctttgc tcacctgcgg tatgggttac tgggtctcgg tgattcagaa
300 tacacctact tttgcaatgg ggggaagata attgataaac gacttcaaga
gcttggagcc 360 cggcatttct atgacactgg acatgcagat gactgtgtag
gtttagaact tgtggttgag 420 ccgtggattg ctggactctg gccagccctc
agaaagcatt ttaggtcaag cagaggacaa 480 gaggagataa gtggcgcact
cccggtggca tcacctgcat ccttgaggac agaccttgtg 540 aagtcagagc
tgctacacat tgaatctcaa gtcgagcttc tgagattcga tgattcagga 600
agaaaggatt ctgaggtttt gaagcaaaat gcagtgaaca gcaaccaatc caatgttgta
660 attgaagact ttgagtcctc acttacccgt tcggtacccc cactctcaca
agcctctctg 720 aatattcctg gtttaccccc agaatattta caggtacatc
tgcaggagtc tcttggccag 780 gaggaaagcc aagtatctgt gacttcagca
gatccagttt ttcaagtgcc aatttcaaag 840 gcagttcaac ttactacgaa
tgatgccata aaaaccactc tgctggtaga attggacatt 900 tcaaatacag
acttttccta tcagcctgga gatgccttca gcgtgatctg ccctaacagt 960
gattctgagg tacaaagcct actccaaaga ctgcagcttg aagataaaag agagcactgc
1020 gtccttttga aaataaaggc agacacaaag aagaaaggag ctaccttacc
ccagcatata 1080 cctgcgggat gttctctcca gttcattttt acctggtgtc
ttgaaatccg agcaattcct 1140 aaaaaggcat ttttgcgagc ccttgtggac
tataccagtg acagtgctga aaagcgcagg 1200 ctacaggagc tgtgcagtaa
acaaggggca gccgattata gccgctttgt acgagatgcc 1260 tgtgcctgct
tgttggatct cctcctcgct ttcccttctt gccagccacc actcagtctc 1320
ctgctcgaac atcttcctaa acttcaaccc agaccatatt cgtgtgcaag ctcaagttta
1380 tttcacccag gaaagctcca ttttgtcttc aacattgtgg aatttctgtc
tactgccaca 1440 acagaggttc tgcggaaggg agtatgtaca ggctggctgg
ccttgttggt tgcttcagtt 1500 cttcagccaa acatacatgc atcccatgaa
gacagcggga aagccctggc tcctaagata 1560 tccatctctc ctcgaacaac
aaattctttc cacttaccag atgacccctc aatccccatc 1620 ataatggtgg
gtccaggaac cggcatagcc ccgtttattg ggttcctaca acatagaaac 1680
tccaagaaca acacccagat ggaaattttg gagcaatgtg gttgtttttt ggctgcaggc
1740 ataaggatag ggattatcta ttcagaaaag agctcagaca tttccttaag
catgggatct 1800 taactcatct aaaggtttcc ttctcaagag atgctcctgt
tggggaggag gaagccccag 1860 caaagtatgt acaagacaac atccagcttc
atggccagca ggtggcgaga atcctcctcc 1920 aggagaacgg ccatatttat
gtgtgtggag atgcaaagaa tatggccaag gatgtacatg 1980 atgcccttgt
gcaaataata agcaaagagg ttggagttga aaaactagaa gcaatgaaaa 2040
ccctggccac tttaaaagaa gaaaaacgct accttcagga tatttggtca taa 2093 48
689 PRT Homo sapiens 48 Arg Arg Phe Leu Leu Leu Tyr Ala Thr Gln Gln
Gly Gln Ala Lys Ala 1 5 10 15 Ile Ala Glu Glu Met Cys Glu Gln Ala
Val Val His Gly Phe Ser Ala 20 25 30 Asp Leu His Cys Ile Ser Glu
Ser Asp Lys Tyr Asp Leu Lys Thr Glu 35 40 45 Thr Ala Pro Leu Val
Val Val Val Ser Thr Thr Gly Thr Gly Asp Pro 50 55 60 Pro Asp Thr
Ala Arg Lys Phe Val Lys Glu Ile Gln Asn Gln Thr Leu 65 70 75 80 Pro
Val Asp Phe Phe Ala His Leu Arg Tyr Gly Leu Leu Gly Leu Gly 85 90
95 Asp Ser Glu Tyr Thr Tyr Phe Cys Asn Gly Gly Lys Ile Ile Asp Lys
100 105 110 Arg Leu Gln Glu Leu Gly Ala Arg His Phe Tyr Asp Thr Gly
His Ala 115 120 125 Asp Asp Cys Val Gly Leu Glu Leu Val Val Glu Pro
Trp Ile Ala Gly 130 135 140 Leu Trp Pro Ala Leu Arg Lys His Phe Arg
Ser Ser Arg Gly Gln Glu 145 150 155 160 Glu Ile Ser Gly Ala Leu Pro
Val Ala Ser Pro Ala Ser Leu Arg Thr 165 170 175 Asp Leu Val Lys Ser
Glu Leu Leu His Ile Glu Ser Gln Val Glu Leu 180 185 190 Leu Arg Phe
Asp Asp Ser Gly Arg Lys Asp Ser Glu Val Leu Lys Gln 195 200 205 Asn
Ala Val Asn Ser Asn Gln Ser Asn Val Val Ile Glu Asp Phe Glu 210 215
220 Ser Ser Leu Thr Arg Ser Val Pro Pro Leu Ser Gln Ala Ser Leu Asn
225 230 235 240 Ile Pro Gly Leu Pro Pro Glu Tyr Leu Gln Val His Leu
Gln Glu Ser 245 250 255 Leu Gly Gln Glu Glu Ser Gln Val Ser Val Thr
Ser Ala Asp Pro Val 260 265 270 Phe Gln Val Pro Ile Ser Lys Ala Val
Gln Leu Thr Thr Asn Asp Ala 275 280 285 Ile Lys Thr Thr Leu Leu Val
Glu Leu Asp Ile Ser Asn Thr Asp Phe 290 295 300 Ser Tyr Gln Pro Gly
Asp Ala Phe Ser Val Ile Cys Pro Asn Ser Asp 305 310 315 320 Ser Glu
Val Gln Ser Leu Leu Gln Arg Leu Gln Leu Glu Asp Lys Arg 325 330 335
Glu His Cys Val Leu Leu Lys Ile Lys Ala Asp Thr Lys Lys Lys Gly 340
345 350 Ala Thr Leu Pro Gln His Ile Pro Ala Gly Cys Ser Leu Gln Phe
Ile 355 360 365 Phe Thr Trp Cys Leu Glu Ile Arg Ala Ile Pro Lys Lys
Ala Phe Leu 370 375 380 Arg Ala Leu Val Asp Tyr Thr Ser Asp Ser Ala
Glu Lys Arg Arg Leu 385 390 395 400 Gln Glu Leu Cys Ser Lys Gln Gly
Ala Ala Asp Tyr Ser Arg Phe Val 405 410 415 Arg Asp Ala Cys Ala Cys
Leu Leu Asp Leu Leu Leu Ala Phe Pro Ser 420 425 430 Cys Gln Pro Pro
Leu Ser Leu Leu Leu Glu His Leu Pro Lys Leu Gln 435 440 445 Pro Arg
Pro Tyr Ser Cys Ala Ser Ser Ser Leu Phe His Pro Gly Lys 450 455 460
Leu His Phe Val Phe Asn Ile Val Glu Phe Leu Ser Thr Ala Thr Thr 465
470 475 480 Glu Val Leu Arg Lys Gly Val Cys Thr Gly Trp Leu Ala Leu
Leu Val 485 490 495 Ala Ser Val Leu Gln Pro Asn Ile His Ala Ser His
Glu Asp Ser Gly 500 505 510 Lys Ala Leu Ala Pro Lys Ile Ser Ile Ser
Pro Arg Thr Thr Asn Ser 515 520 525 Phe His Leu Pro Asp Asp Pro Ser
Ile Pro Ile Ile Met Val Gly Pro 530 535 540 Gly Thr Gly Ile Ala Pro
Phe Ile Gly Phe Leu Gln His Arg Asn Ser 545 550 555 560 Lys Asn Asn
Thr Gln Met Glu Ile Leu Glu Gln Cys Gly Cys Phe Leu 565 570 575 Ala
Ala Gly Ile Arg Ile Gly Ile Ile Tyr Ser Glu Lys Ser Ser Asp 580 585
590 Ile Ser Leu Ser Met Gly Ser Leu Ile Arg Phe Pro Ser Gln Glu Met
595 600 605 Leu Leu Leu Gly Arg Arg Lys Pro Gln Gln Ser Met Tyr Lys
Thr Thr 610 615 620 Ser Ser Phe Met Ala Ser Arg Trp Arg Glu Ser Ser
Ser Arg Arg Thr 625 630 635 640 Ala Ile Phe Met Cys Val Glu Met Gln
Arg Ile Trp Pro Arg Met Tyr 645 650 655 Met Met Pro Leu Cys Lys Ala
Lys Arg Leu Glu Leu Lys Asn Lys Gln 660 665 670 Lys Pro Trp Pro Leu
Lys Lys Lys Asn Ala Thr Phe Arg Ile Phe Gly 675 680 685 His 49 23
DNA Homo sapiens 49 gcaaaggcca tcgcagaaga cat 23 50 26 DNA Homo
sapiens 50 gtgaagatct gcagaaaatc catgta 26 51 2187 DNA Homo sapiens
51 gccatggtga acgaagccag aggaaacagc agcctcaacc cctgcttgga
gggcagtgcc 60 agcagtggca gtgagagctc caaagatagt tcgagatgtt
ccaccccggg cctggaccct 120 gagcggcatg agagactccg ggagaagatg
aggcggcgat tggaatctgg tgacaagtgg 180 ttctccctgg aattcttccc
tcctcgaact gctgagggag ctgtcaatct catctcaagg 240 tttgaccgga
tggcagcagg tggccccctc tacatagacg tgacctggca cccagcaggt 300
gaccctggct cagacaagga gacctcctcc atgatgatcg ccagcaccgc cgtgaactac
360 tgtggcctgg agaccatcct gcacatgacc tgctgccgtc agcgcctgga
ggagatcacg 420 ggccatctgc acaaagctaa gcagctgggc ctgaagaaca
tcatggcgct gcggggagac 480 ccaataggtg accagtggga agaggaggag
ggaggcttca actacgcagt ggacctggtg 540 aagcacatcc gaagtgagtt
tggtgactac tttgacatct gtgtggcagg ttaccccaaa 600 ggccaccccg
aagcagggag ctttgaggct gacctgaagc acttgaagga gaaggtgtct 660
gcgggagccg atttcatcat cacgcagctt ttctttgagg ctgacacatt cttccgcttt
720 gtgaaggcat gcaccgacat gggcatcact tgccccatcg tccccgggat
ctttcccatc 780 cagggctacc actcccttcg gcagcttgtg aagctgtcca
agctggaggt gccacaggag 840 atcaaggacg tgattgagcc aatcaaagac
aacgatgctg ccatccgcaa ctatggcatc 900 gagctggccg tgagcctgtg
ccaggagctt ctggccagtg gcttggtgcc aggcctccac 960 ttctacaccc
tcaaccgcga gatggctacc acagaggtgc tgaagcgcct ggggatgtgg 1020
actgaggacc ccaggcgtcc cctaccctgg gctctcagtg cccaccccaa gcgccgagag
1080 gaagatgtac gtcccatctt ctgggcctcc agaccaaaga gttacatcta
ccgtacccag 1140 gagtgggacg agttccctaa cggccgctgg ggcaattcct
cttcccctgc ctttggggag 1200 ctgaaggact actacctctt ctacctgaag
agcaagtccc ccaaggagga gctgctgaag 1260 atgtgggggg aggagctgac
cagtgaagca agtgtctttg aagtctttgt tctttacctc 1320 tcgggagaac
caaaccggaa tggtcacaaa gtgacttgcc tgccctggaa cgatgagccc 1380
ctggcggctg agaccagcct gctgaaggag gagctgctgc gggtgaaccg ccagggcatc
1440 ctcaccatca actcacagcc caacatcaac gggaagccgt cctccgaccc
catcgtgggc 1500 tggggcccca gcgggggcta tgtcttccag aaggcctact
tagagttttt cacttcccgc 1560 gagacagcgg aagcacttct gcaagtgctg
aagaagtacg agctccgggt taattaccac 1620 cttgtcaatg tgaagggtga
aaacatcacc aatgcccctg aactgcagcc gaatgctgtc 1680 acttggggca
tcttccctgg gcgagagatc atccagccca ccgtagtgga tcccgtcagc 1740
ttcatgttct ggaaggacga ggcctttgcc ctgtggattg agcggtgggg aaagctgtat
1800 gaggaggagt ccccgtcccg caccatcatc cagtacatcc acgacaacta
cttcctggtc 1860 aacctggtgg acaatgactt cccactggac aactgcctct
ggcaggtggt ggaagacaca 1920 ttggagcttc tcaacaggcc cacccagaat
gcgagagaaa cggaggctcc atgaccctgc 1980 gtcctgacgc cctgcgttgg
agccactcct gtcccgcctt cctcctccac agtgctgctt 2040 ctcttgggaa
ctccactctc cttcgtgtct ctcccacccc ggcctccact cccccacctg 2100
acaatggcag ctagactgga gtgaggcttc caggctcttc ctggacctga gtcggcccca
2160 catgggaacc tagtactctc tgctcta 2187 52 20 PRT Homo sapiens 52
Phe Leu Leu Leu Tyr Ala Thr Gln Gln Gly Gln Ala Lys Ala Ile Ala 1 5
10 15 Glu Glu Met Cys 20 53 23 PRT Homo sapiens 53 Val Val Val Val
Ser Thr Thr Gly Thr Gly Asp Pro Pro Asp Thr Ala 1 5 10 15 Arg Lys
Phe Val Lys Glu Ile 20 54 29 PRT Homo sapiens 54 Ala His Leu Arg
Tyr Gly Leu Leu Gly Leu Gly Asp Ser Glu Tyr Thr 1 5 10 15 Tyr Phe
Cys Asn Gly Gly Lys Ile Ile Asp Lys Arg Leu 20 25 55 19 PRT Homo
sapiens 55 Leu Gln Pro Arg Pro Tyr Ser Cys Ala Ser Ser Ser Leu Phe
His Pro 1 5 10 15 Gly Lys Leu 56 14 PRT Homo sapiens 56 Phe Val Phe
Asn Ile Val Glu Phe Leu Ser Thr Ala Thr Thr 1 5 10 57 17 PRT Homo
sapiens 57 Leu Arg Lys Gly Val Cys Thr Gly Trp Leu Ala Leu Leu Val
Ala Ser 1 5 10 15 Val 58 22 PRT Homo sapiens 58 Ile Pro Ile Ile Met
Val Gly Pro Gly Thr Gly Ile Ala Pro Phe Ile 1 5 10 15 Gly Phe Leu
Gln His Arg 20 59 6 PRT Homo sapiens 59 Ser Phe Ser Arg Asp Ala 1 5
60 41 PRT Homo sapiens 60 Ala Pro Ala Lys Tyr Val Gln Asp Asn Ile
Gln Leu His Gly Gln Gln 1 5 10 15 Val Ala Arg Ile Leu Leu Gln Glu
Asn Gly His Ile Tyr Val Cys Gly 20 25 30 Asp Ala Lys Asn Met Ala
Lys Asp Val 35 40 61 26 DNA Homo sapiens 61 caggcaaagg ccatcgcaga
agacat 26 62 27 DNA Homo sapiens 62 cacttcccaa ccaaaattct tcaaaag
27 63 9 PRT Homo sapiens 63 Lys Arg Tyr Leu Gln Asp Ile Trp Ser 1
5
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