U.S. patent application number 11/109017 was filed with the patent office on 2005-08-25 for cell proliferation inhibitors.
Invention is credited to Barr, Kenneth J., Gwaltney, Stephen L. II, Imade, Hovis M., Li, Qun, Rosenberg, Saul, Sham, Hing, Steiner, Beth A., Woods, Keith W..
Application Number | 20050187246 11/109017 |
Document ID | / |
Family ID | 22473292 |
Filed Date | 2005-08-25 |
United States Patent
Application |
20050187246 |
Kind Code |
A1 |
Li, Qun ; et al. |
August 25, 2005 |
Cell proliferation inhibitors
Abstract
Compounds having formula (I) 1 inhibit cellular proliferation.
Processes for the preparation of the compounds, pharmaceutical
compositions containing the compounds, and methods of treatment
using the compounds are disclosed.
Inventors: |
Li, Qun; (Libertyville,
IL) ; Sham, Hing; (Mundelein, IL) ; Woods,
Keith W.; (Libertyville, IL) ; Steiner, Beth A.;
(Remington, IN) ; Gwaltney, Stephen L. II;
(Lindenhurst, IL) ; Barr, Kenneth J.; (San
Francisco, CA) ; Imade, Hovis M.; (Chicago, IL)
; Rosenberg, Saul; (Grayslake, IL) |
Correspondence
Address: |
ROBERT DEBERARDINE
ABBOTT LABORATORIES
100 ABBOTT PARK ROAD
DEPT. 377/AP6A
ABBOTT PARK
IL
60064-6008
US
|
Family ID: |
22473292 |
Appl. No.: |
11/109017 |
Filed: |
April 19, 2005 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11109017 |
Apr 19, 2005 |
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10301427 |
Nov 21, 2002 |
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10301427 |
Nov 21, 2002 |
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09579705 |
May 26, 2000 |
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6521658 |
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60136542 |
May 28, 1999 |
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Current U.S.
Class: |
514/313 ;
514/394; 514/406; 514/419; 514/602; 546/159; 548/465; 548/498;
564/86 |
Current CPC
Class: |
C07C 323/29 20130101;
C07C 311/29 20130101; C07C 317/22 20130101; C07C 311/21 20130101;
C07D 209/04 20130101; A61P 35/00 20180101; C07C 323/20 20130101;
C07C 309/75 20130101; C07C 235/56 20130101; C07C 309/76 20130101;
C07D 209/08 20130101; C07C 317/28 20130101; A61P 43/00 20180101;
C07C 311/44 20130101 |
Class at
Publication: |
514/313 ;
514/602; 564/086; 514/419; 548/498; 514/394; 514/406; 546/159;
548/465 |
International
Class: |
A61K 031/4709; A61K
031/4184; A61K 031/405; A61K 031/18 |
Claims
What is claimed is:
1. A compound having formula (I) 7or pharmaceutically acceptable
salts or prodrugs thereof, wherein L.sup.1 is selected from the
group consisting of (1) --S(O).sub.2O--, (2) --OS(O).sub.2--, (3)
--NR.sup.7SO.sub.2--, wherein R.sup.7 is selected from the group
consisting of (a) hydrogen, (b) hydroxy, (c) amidinyl, (d) a
nitrogen-protecting group, (e) alkanoyl, (f) alkyl, (g) alkenyl,
(h) alkynyl, (i) cycloalkyl, (j) cycloalkylalkyl, (k) cycloalkenyl,
(l) cycloalkenylalkyl, (m) aryloyl, (n) alkoxy, wherein (e)-(n) can
be optionally substituted with one, two, or three substituents
independently selected from the group consisting of (i) hydroxyl,
(ii) halo, (iii) cyano, (iv) azido, (v) carboxy, (vi) amidinyl,
(vii) alkyl, (viii) aryl, (ix) oxo, (x) heteroaryl, (xi)
heterocycloalkyl, (xii) --NR.sup.cR.sup.d, wherein R.sup.c and
R.sup.d are independently selected from the group consisting of
(1') hydrogen, (2') alkyl, (3') aryl, and (4') alkoxyalkyl, and
(xiii) -(alkylene)--NR.sup.cR.sup.d, wherein for (x) and (xi), the
heteroaryl and the heterocycloalkyl can be optionally substituted
with 1, 2, or 3 substituents independently selected from the group
consisting of (1') alkyl, and (2') a nitrogen protecting group, (o)
heterocycloalkyloyl, wherein the heterocycloalkyloyl can be
optionally substituted with 1, 2, or 3 substituents independently
selected from the group consisting of (i) alkyl, and (ii) a
nitrogen protecting group, and (p)
--(CH.sub.2).sub.xNR.sup.AR.sup.B, wherein x is 0-6, and R.sup.A
and R.sup.B are independently selected from the group consisting of
(i) hydrogen, (ii) alkyl, (iii) alkenyl, (iv) alkynyl, (v)
cycloalkyl, (vi) cycloalkylalkyl, (vii) cycloalkenyl, and (viii)
cycloalkenylalkyl, (4) --SO.sub.2NR.sup.7--, wherein R.sup.7 is
defined above, (5) --S(O)CR.sup.12R.sup.13--, wherein R.sup.12 and
R.sup.13 are independently selected from the group consisting of
(a) hydrogen, (b) alkyl, (c) alkenyl, and (d) alkynyl, (6)
--SO.sub.2CR.sup.12R.sup.13--, (7) --SCR.sup.12R.sup.13--, (8)
--CR.sup.12R.sup.13S(O)--, (9) --CR.sup.12R.sup.13SO.sub.2--, and
(10) --CR.sup.12R.sup.13S--, wherein (1)-(10) are shown with their
left ends attached to R.sup.1 and their right ends attached to the
phenyl ring; R.sup.1 is aryl or heteroaryl, wherein the aryl or the
heteroaryl can be optionally substituted with 1, 2, 3, 4,or 5
substituents independently selected from the group consisting of
(a) oxo, (b) azido, (c) carboxy, (d) carboxaldehyde, (e) cyano, (f)
halo, (g) hydroxy, (h) nitro, (i) perfluoroalkyl, (j)
perfluoroalkoxy, (k) alkyl, (l) alkenyl, (m) alkynyl, (n)
alkanoyloxy, (o) alkoxycarbonyl, (p) cycloalkyl, (q)
cycloalkylalkyl, (r) cycloalkenyl, (s) cycloalkenylalkyl, (t)
alkanoyl, (u) alkoxy, (v) cycloalkoxy, (w) aryloxy, (x)
heteroaryloxy, (y) thioalkoxy (z) alkylsulfinyl, (aa)
alkylsulfonyl, (bb) --NR.sup.8R.sup.9, wherein R.sup.8 and R.sup.9
are independently selected from the group consisting of (i)
hydrogen (ii) alkyl, (iii) arylalkyl, and (iv) alkanoyl, wherein
the alkanoyl can be optionally substituted with 1 or 2 substituents
independently selected from the group consisting of (1') halo (2')
hydroxy, and (3') --NR.sup.10R.sup.11 wherein R.sup.10 and R.sup.11
are independently hydrogen or alkyl, and (cc)
--SO.sub.2NR.sup.8R.sup.9, wherein R.sup.8 and R.sup.9 are defined
above; R.sup.2 and R.sup.6 are independently selected from the
group consisting of (1) hydrogen, (2) alkyl, (3) alkoxy, (4)
thioalkoxy; and (5) hydroxy, and R.sup.3, R.sup.4, and R.sup.5 are
independently selected from the group consisting of (1) alkyl, (2)
alkoxy, (3) thioalkoxy, and (4) hydroxy; all of the foregoing with
the proviso that combinations wherein L.sup.1 is
--NR.sup.7SO.sub.2-- and R.sup.1 is (1) unsubstituted or
substituted 1H-indoly-7-yl, (2) phenyl which is 2-monosubstituted
with --NR.sup.8R.sup.9, (3) pyrid-3-yl which is 2-monosubstituted
with --NR.sup.8R.sup.9, or (4) pyrimidin-5-yl which is
4-monosubstituted with --NR.sup.8R.sup.9, are excluded
therefrom.
2. A compound according to claim 1, wherein L.sup.1 is
--SO.sub.2NR.sup.7--, and R.sup.7 is defined above.
3. A compound according to claim 2, wherein R.sup.2 and R.sup.6 are
hydrogen, and R.sup.3, R.sup.4, and R.sup.5 are methoxy.
4. A compound according to claim 3, wherein R.sup.1 is optionally
substituted aryl.
5. A compound according to claim 4 selected from the group
consisting of
4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3,4-dimethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-amino-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-((2-chloroacetyl)amino)-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
2-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3-amino-4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide, and
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide.
6. A compound according to claim 3, wherein R.sup.1 is optionally
substituted heteroaryl.
7. A compound according to claim 6, wherein R.sup.1 is optionally
substituted 2,3-dihydro-1H-indol-5-yl.
8. A compound according to claim 7 selected from the group
consisting of
1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide, and
1-methyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide.
9. A compound according to claim 6, wherein R.sup.1 is optionally
substituted 1H-indol-3-yl.
10. A compound according to claim 9 selected from the group
consisting of
5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
5-amino-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
and 5-amino-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide.
11. A compound according to claim 6, wherein R.sup.1 is optionally
substituted 1H-indol-5-yl.
12. A compound according to claim 11 selected from the group
consisting of N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N,1-dimethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-ethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-(2-hydroxyethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide,
N-(2-fluoroethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-s-
ulfonamide,
N-ethyl-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide,
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-in-
dole-5-sulfonamide,
1-methyl-N-(((2S)-1-methylpyrrolidinyl)carbonyl)-N-(3,-
4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-(dimethylamino)-3-
-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonami-
de,
N-((2S)-2-amino-3-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)--
1H-indole-5-sulfonamide,
1-methyl-N-((2S)-2-methylamino)propanoyl)-N-(3,4,-
5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((.sup.2S)-2-amino-2-phenyl-
ethanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-phenylpropanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-
-indole-5-sulfonamide,
1-methyl-N-((2S)-pyrrolidinylcarbonyl)-N-(3,4,5-tri-
methoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2,6-diaminohexanoyl)-1-met-
hyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl)-1-methyl-N-(3,4,5-trimetho-
xyphenyl)-1H-indole-5-sulfonamide,
(2S)-2-amino-4-oxo-4-(3,4,5-trimethoxy(-
(1-methyl-1H-indol-5-yl)sulfonyl)anilino)butanoic acid,
(3S)-3-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)a-
nilino)butanoic acid,
(2S)-2-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H--
indol-5-yl)sulfonyl)anilino)pentanoic acid,
(4S)-4-amino-5-oxo-5-(3,4,5-tr-
imethoxy((1-methyl-1H-indol-5-yl)sulfonyl)anilino)pentanoic acid,
N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)--
1H-indole-5-sulfonamide,
1-methyl-N-(4-morpholinylacetyl)-N-(3,4,5-trimeth-
oxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((4-methyl-1-piperazinyl)ac-
etyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole--
5-sulfonamide,
N-(2-aminoacetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-in-
dole-5-sulfonamide,
N-((.sup.2S)-2-aminopropanoyl]-1-methyl-N-(3,4,5-trime-
thoxyphenyl)-1H-indole-5-sulfonamide,
N-(3-aminopropanoyl)-1-methyl-N-(3,4-
,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
(2S)-2-amino-N-((1S)-1-methy-
l-2-oxo-2-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)anilino)ethyl-
)propanamide, and
N-((2S)-2-amino-3-hydroxypropanoyl)-1-methyl-N-(3,4,5-tr-
imethoxyphenyl)-1H-indole-5-sulfonamide.
13. A compound according to claim 1, wherein L.sup.1 is
--NR.sup.7SO.sub.2--, and R.sup.7 is defined above.
14. A compound according to claim 13, wherein R.sup.2 and R.sup.6
are hydrogen, and R.sup.3, R.sup.4, and R.sup.5 are methoxy.
15. A compound according to claim 14 selected from the group
consisting of
3,4,5-trimethoxy-N-(4-methoxyphenyl)benzenesulfonamide,
N-(3-hydroxy-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1-methyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-(dimethylamino)phenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-fluoro-3-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(4-(trifluoromethoxy)phenyl)benzenesulfonamide,
3,4,5-trimethoxy-N-(2,3,4,5,6-pentafluorophenyl)benzenesulfonamide,
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-4-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-6-yl)benzenesulfonamide,
N-(1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(3-chloro-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1H-indazol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-6-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-methyl-N-(1-methyl-1H-indol-5-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(2-(dimethylamino)ethyl)-N-(1-methyl-1H-indol-5-yl)ben-
zenesulfonamide, tert-butyl
2-((1-methyl-1H-indol-5-yl)((3,4,5-trimethoxyp-
henyl)sulfonyl)amino)ethylcarbamate,
N-(2-hydroxyethyl)-3,4,5-trimethoxy-N-
-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
N-(2,3-dihydro-1,4-benzodioxi-
n-6-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(2-aminoethyl)-3,4,5-trimeth-
oxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
N-acetyl-3,4,5-trimethox-
y-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(6-quin- olinyl)benzenesulfonamide,
N-(2-aminoacetyl)-3,4,5-trimethoxy-N-(1-methyl--
1H-indol-5-yl)benzenesulfonamide,
N-((2S)-2-aminopropanoyl]-3,4,5-trimetho-
xy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
N-(3-aminopropanoyl)-3,4,-
5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
(2S)-2-amino-N-((1S)-1-methyl-2-((1-methyl-1H-indol-5-yl)((3,4,5-trimetho-
xyphenyl)sulfonyl)amino)-2-oxoethyl)propanamide, and
N-((2S)-2-amino-3-hydroxypropanoyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-
-5-yl)benzenesulfonamide.
16. A compound according to claim 1, wherein L.sup.1 is
--OSO.sub.2--.
17. A compound according to claim 16, wherein R.sup.2 and R.sup.6
are hydrogen, and R.sup.3, R.sup.4, and R.sup.5 are methoxy.
18. A compound according to claim 17 selected from the group
consisting of 1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate,
1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate, 4-methylphenyl
3,4,5-trimethoxybenzenes- ulfonate, 4-methoxyphenyl
3,4,5-trimethoxybenzenesulfonate,
4-nitrophenyl-3,4,5-trimethoxybenzenesulfonate,
4-aminophenyl-3,4,5-trime- thoxybenzenesulfonate,
4-dimethylaminophenyl-3,4,5-trimethoxybenzenesulfon- ate, and
1-methyl-2-oxo-1,2-dihydro-4-pyridinyl 3,4,5-trimethoxybenzenesul-
fonate.
19. A compound according to claim 1, wherein L.sup.1 is
--SO.sub.2O--.
20. A compound according to claim 19, wherein R.sup.2 and R.sup.6
are hydrogen, and R.sup.3, R.sup.4, and R.sup.5 are methoxy.
21. A compound according to claim 20 selected from the group
consisting of (3,4,5-trimethoxyphenyl) 4-methoxybenzenesulfonate,
3,4,5-trimethoxyphenyl) 4-methylbenzenesulfonate,
3,4,5-trimethoxyphenyl) 3-amino-4-methoxybenzenesulfonate,
(3,4,5-trimethoxyphenyl)-4-(dimethylam- ino)benzenesulfonate,
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate,
3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate,
3,4,5-trimethoxyphenyl
3-((3-aminopropanoyl)amino)-4-methoxybenzenesulfon- ate,
3,4,5-trimethoxyphenyl
3-(((2R)-2-aminopropanoyl)amino)-4-methoxybenz- enesulfonate, and
3,4,5-trimethoxyphenyl 3-(((2R)-2-amino-3-methylbutanoyl-
)amino)-4-methoxybenzenesulfonate.
22. A compound according to claim 1, wherein L.sup.1 is selected
from the group consisting of --SO.sub.2CR.sup.12R.sup.13--,
--SCR.sup.12R.sup.13--, --CR.sup.12R.sup.13S(O)--,
--CR.sup.12R.sup.13SO.sub.2--, and --CR.sup.12 R.sup.13S--.
23. A compound according to claim 22 selected from the group
consisting of
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfanyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfinyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfonyl)benzene,
1,2,3-trimethoxy-5-((1-(4-methoxyphenyl)-1-methylethyl)sulfonyl)benzene,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline,
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)aniline,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfanyl)methyl)benzene,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfonyl)methyl)benzene,
1,2,3-trimethoxy-5-(1-((4-methoxyphenyl)sulfonyl)-1-methylethyl)benzene,
2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline,
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)aniline,
and 1,2,3-trimethoxy-5-((phenylsulfonyl)methyl)benzene.
24. A method of inhibiting polymerization of tubulin in a mammal in
recognized need of such treatment comprising administering an
effective amount of a compound of claim 1.
25. A method of treating cancer in a mammal in recognized need of
such treatment comprising administering an effective amount of a
compound of claim 1.
26. A compound selected from the group consisting of
4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3,4-dimethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-amino-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-((2-chloroacetyl)amino)-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
2-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3-amino-4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
5-nitro-N-(3,4,5-trimet- hoxyphenyl)-1H-indole-3-sulfonamide,
1-methyl-N-(3,4,5-trimethoxyphenyl)in- doline-5-sulfonamide,
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indol-
e-3-sulfonamide,
5-amino-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-s-
ulfonamide,
5-amino-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N,1-dimethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
3,4,5-trimethoxy-N-(4-methoxyphenyl)benzenesulfonamide,
N-(3-hydroxy-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1-methyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-(dimethylamino)phenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-fluoro-3-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(4-(trifluoromethoxy)phenyl)benzenesulfonamide,
3,4,5-trimethoxy-N-(2,3,4,5,6-pentafluorophenyl)benzenesulfonamide,
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-4-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-6-yl)benzenesulfonamide,
N-(1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(3-chloro-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1H-indazol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-6-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-methyl-N-(1-methyl-1H-indol-5-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(2-(dimethylamino)ethyl)-N-(1-methyl-1H-indol-5-yl)ben-
zenesulfonamide, 1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate,
(3,4,5-trimethoxyphenyl) 4-methoxybenzenesulfonate,
3,4,5-trimethoxyphenyl) 4-methylbenzenesulfonate, 1H-indol-5-yl
3,4,5-trimethoxybenzenesulfonate, 3,4,5-trimethoxyphenyl)
3-amino-4-methoxybenzenesulfonate,
(3,4,5-trimethoxyphenyl)-4-(dimethylam- ino)benzenesulfonate,
4-methylphenyl 3,4,5-trimethoxybenzenesulfonate,
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate, and
4-methoxyphenyl 3,4,5-trimethoxybenzenesulfonate, tert-butyl
2-((1-methyl-1H-indol-5-yl)(-
(3,4,5-trimethoxyphenyl)sulfonyl)amino)ethylcarbamate,
N-(2-hydroxyethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulf-
onamide,
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3,4,5-trimethoxybenzenesulfo-
namide,
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzen-
esulfonamide,
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzene-
sulfonamide,
1-ethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-acetyl-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(6-quinolinyl)benzenesulfonamide,
N-(2-hydroxyethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide,
N-(2-fluoroethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-s-
ulfonamide,
N-ethyl-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide, 4-nitrophenyl-3,4,5-trimethoxybenzenesulfonate,
4-aminophenyl-3,4,5-trimethoxybenzenesulfonate,
4-dimethylaminophenyl-3,4- ,5-trimethoxybenzenesulfonate,
3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate,
1-methyl-2-oxo-1,2-dihydro-4-pyridinyl
3,4,5-trimethoxybenzenesulfonate, 3,4,5-trimethoxyphenyl
3-((3-aminopropanoyl)amino)-4-methoxybenzenesulfonate,
3,4,5-trimethoxyphenyl
3-(((2R)-2-aminopropanoyl)amino)-4-methoxybenzenes- ulfonate,
3,4,5-trimethoxyphenyl 3-(((2R)-2-amino-3-methylbutanoyl)amino)--
4-methoxybenzenesulfonate,
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-tri-
methoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(((2S)-1-methylpyrrolid-
inyl)carbonyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-(dimethylamino)-3-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxyp-
henyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-methylbutanoyl)-1-methyl-
-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((2S)-2-methylamino)propanoyl)-N-(3,4,5-trimethoxyphenyl)1H-in-
dole-5-sulfonamide,
N-((2S)-2-amino-2-phenylethanoyl)-1-methyl-N-(3,4,5-tr-
imethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-phenylpropanoyl-
)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((2S)-pyrrolidinylcarbonyl)-N-(3,4,5-trimethoxyphenyl)-1H-indo-
le-5-sulfonamide,
N-((2S)-2,6-diaminohexanoyl)-1-methyl-N-(3,4,5-trimethox-
yphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-(1H-imidazol-5-yl)prop-
anoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
(2S)-2-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)a-
nilino)butanoic acid,
(3S)-3-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H--
indol-5-yl)sulfonyl)anilino)butanoic acid,
(2S)-2-amino-5-oxo-5-(3,4,5-tri-
methoxy((1-methyl-1H-indol-5-yl)sulfonyl)anilino)pentanoic acid,
(4S)-4-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)a-
nilino)pentanoic acid,
N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl-N-(3,-
4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(4-morpholinylac-
etyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((4-methyl-1-piperazinyl)acetyl)-N-(3,4,5-trimethoxyphenyl)-1H-
-indole-5-sulfonamide,
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimet-
hoxyphenyl)-1H-indole-5-sulfonamide,
1,2,3-trimethoxy-5-((4-methoxybenzyl)- sulfanyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfinyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfonyl)benzene,
1,2,3-trimethoxy-5-((1-(4-methoxyphenyl)-1-methylethyl)sulfonyl)benzene,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline,
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)aniline,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfanyl)methyl)benzene,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfonyl)methyl)benzene,
1,2,3-trimethoxy-5-(1-((4-methoxyphenyl)sulfonyl)-1-methylethyl)benzene,
2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline,
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)aniline,
1,2,3-trimethoxy-5-((phenylsulfonyl)methyl)benzene,
N-(2-aminoacetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfona-
mide,
N-(2-aminoacetyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzene-
sulfonamide,
N-((2S)-2-aminopropanoyl]-1-methyl-N-(3,4,5-trimethoxyphenyl)-
-1H-indole-5-sulfonamide,
N-((2S)-2-aminopropanoyl]-3,4,5-trimethoxy-N-(1--
methyl-1H-indol-5-yl)benzenesulfonamide,
N-(3-aminopropanoyl)-1-methyl-N-(-
3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-(3-aminopropanoyl)-3,4,-
5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
(2S)-2-amino-N-((1S)-1-methyl-2-oxo-2-(3,4,5-trimethoxy((1-methyl-1H-indo-
l-5-yl)sulfonyl)anilino)ethyl)propanamide,
(2S)-2-amino-N-((1S)-1-methyl-2-
-((1-methyl-1H-indol-5-yl)((3,4,5-trimethoxyphenyl)sulfonyl)amino)-2-oxoet-
hyl)propanamide,
N-((2S)-2-amino-3-hydroxypropanoyl)-1-methyl-N-(3,4,5-tri-
methoxyphenyl)-1H-indole-5-sulfonamide, and
N-((2S)-2-amino-3-hydroxypropa-
noyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide.
27. A compound selected from the group consisting of
4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3,4-dimethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-trifluoromethyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-amino-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-((2-chloroacetyl)amino)-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
2-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
3-amino-4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
5-nitro-N-(3,4,5-trimet- hoxyphenyl)-1H-indole-3-sulfonamide,
1-methyl-N-(3,4,5-trimethoxyphenyl)in- doline-5-sulfonamide,
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indol-
e-3-sulfonamide,
5-amino-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-s-
ulfonamide,
5-amino-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N,1-dimethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
3,4,5-trimethoxy-N-(4-methoxyphenyl)benzenesulfonamide,
N-(3-hydroxy-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1-methyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-(dimethylamino)phenyl)-3,4,5-trimethoxybenzenesulfonamide,
N-(4-fluoro-3-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(4-(trifluoromethoxy)phenyl)benzenesulfonamide,
3,4,5-trimethoxy-N-(2,3,4,5,6-pentafluorophenyl)benzenesulfonamide,
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-4-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-indol-6-yl)benzenesulfonamide,
N-(1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(3-chloro-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
N-(1H-indazol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-6-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-methyl-N-(1-methyl-1H-indol-5-yl)
benzenesulfonamide,
3,4,5-trimethoxy-N-(2-(dimethylamino)ethyl)-N-(1-methyl-1H-indol-5-yl)ben-
zenesulfonamide, 1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate,
(3,4,5-trimethoxyphenyl) 4-methoxybenzenesulfonate,
3,4,5-trimethoxyphenyl) 4-methylbenzenesulfonate, 1H-indol-5-yl
3,4,5-trimethoxybenzenesulfonate, 3,4,5-trimethoxyphenyl)
3-amino-4-methoxybenzenesulfonate,
(3,4,5-trimethoxyphenyl)-4-(dimethylam- ino)benzenesulfonate,
4-methylphenyl 3,4,5-trimethoxybenzenesulfonate,
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate, and
4-methoxyphenyl 3,4,5-trimethoxybenzenesulfonate, tert-butyl
2-((1-methyl-1H-indol-5-yl)(-
(3,4,5-trimethoxyphenyl)sulfonyl)amino)ethylcarbamate,
N-(2-hydroxyethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulf-
onamide,
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3,4,5-trimethoxybenzenesulfo-
namide,
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzen-
esulfonamide,
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzene-
sulfonamide,
1-ethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-acetyl-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide,
3,4,5-trimethoxy-N-(6-quinolinyl)benzenesulfonamide,
N-(2-hydroxyethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide,
N-(2-fluoroethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-s-
ulfonamide,
N-ethyl-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfon-
amide, 4-nitrophenyl-3,4,5-trimethoxybenzenesulfonate,
4-aminophenyl-3,4,5-trimethoxybenzenesulfonate,
4-dimethylaminophenyl-3,4- ,5-trimethoxybenzenesulfonate,
3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate,
1-methyl-2-oxo-1,2-dihydro-4-pyridinyl
3,4,5-trimethoxybenzenesulfonate. 3,4,5-trimethoxyphenyl
3-((3-aminopropanoyl)amino)-4-methoxybenzenesulfonate,
3,4,5-trimethoxyphenyl
3-(((2R)-2-aminopropanoyl)amino)-4-methoxybenzenes- ulfonate,
3,4,5-trimethoxyphenyl 3-(((2R)-2-amino-3-methylbutanoyl)amino)--
4-methoxybenzenesulfonate,
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-tri-
methoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(((2S)-1-methylpyrrolid-
inyl)carbonyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-(dimethylamino)-3-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxyp-
henyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-methylbutanoyl)-1-methyl-
-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((2S)-2-methylamino)propanoyl)-N-(3,4,5-trimethoxyphenyl)-1H-i-
ndole-5-sulfonamide,
N-((2S)-2-amino-2-phenylethanoyl)-1-methyl-N-(3,4,5-t-
rimethoxyphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-phenylpropanoy-
l)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((2S)-pyrrolidinylcarbonyl)-N-(3,4,5-trimethoxyphenyl)-1H-indo-
le-5-sulfonamide,
N-((2S)-2,6-diaminohexanoyl)-1-methyl-N-(3,4,5-trimethox-
yphenyl)-1H-indole-5-sulfonamide,
N-((2S)-2-amino-3-(1H-imidazol-5-yl)prop-
anoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
(2S)-2-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)a-
nilino)butanoic acid,
(3S)-3-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H--
indol-5-yl)sulfonyl)anilino)butanoic acid,
(2S)-2-amino-5-oxo-5-(3,4,5-tri-
methoxy((1-methyl-1H-indol-5-yl)sulfonyl)anilino)pentanoic acid,
(4S)-4-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)a-
nilino)pentanoic acid,
N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl-N-(3,-
4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-(4-morpholinylac-
etyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
1-methyl-N-((4-methyl-1-piperazinyl)acetyl)-N-(3,4,5-trimethoxyphenyl)-1H-
-indole-5-sulfonamide,
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimet-
hoxyphenyl)-1H-indole-5-sulfonamide,
1,2,3-trimethoxy-5-((4-methoxybenzyl)- sulfanyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfinyl)benzene,
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfonyl)benzene,
1,2,3-trimethoxy-5-((1-(4-methoxyphenyl)-1-methylethyl)sulfonyl)benzene,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline,
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)aniline,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfanyl)methyl)benzene,
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfonyl)methyl)benzene,
1,2,3-trimethoxy-5-(1-((4-methoxyphenyl)sulfonyl)-1-methylethyl)benzene,
2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline,
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)aniline,
and 1,2,3-trimethoxy-5-((phenylsulfonyl)methyl)benzene.
28. A compound which is
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-trimet-
hoxyphenyl)-1H-indole-5-sulfonamide.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application
Ser. No. 10/301,427, filed Nov. 21, 2002, which is a divisional of
Ser. No. 09/579,705, filed May 26, 2000, which is a
continuation-in-part of provisional U.S. Patent Application Ser.
No. 60/136,542, filed May 28, 1999.
TECHNICAL FIELD
[0002] The present invention relates to compounds useful for
treating pathological states, which arise from or are exacerbated
by cell proliferation, to pharmaceutical compositions comprising
these compounds, and to methods of inhibiting cell proliferation in
a mammal.
BACKGROUND OF THE INVENTION
[0003] Neoplastic diseases, characterized by the proliferation of
cells which are not subject to normal cell proliferating controls,
are a major cause of death in humans and other mammals. Cancer
chemotherapy has provided new and more effective drugs to treat
these diseases and has also demonstrated that drugs which disrupt
microtubule synthesis are effective in inhibiting the proliferation
of neoplastic cells.
[0004] Microtubules play a key role in the regulation of cell
architecture, metabolism, and division. The microtubule system of
eucaryotic cells comprises a dynamic assembly and disassembly
matrix in which heterodimers of tubulin polymerize to form
microtubules in both normal and neoplastic cells. Within noeplastic
cells, tubulin is polymerized into microtubules which form the
mitotic spindle. The microtubules are then depolymerized when the
mitotic spindle's use has been fulfilled. Agents which disrupt the
polymerization or depolymerization of microtubules in neoplastic
cells, thereby inhibiting the proliferation of these cells,
comprise some of the most effective cancer chemotherapeutic agents
in use.
[0005] Because of the pivotal role played by cell proliferation,
agents which inhibit microtubule polymerization have been the
subject of active current research for their clinical potential.
See, for example, U.S. Pat. Nos. 5,767,283, 5,721,246, 5,610,320,
FR 2,729,421-A1, and WO96/27295. But there is still a need for
tubulin polymerization-inhibiti- ng compounds with modified or
improved profiles of activity.
SUMMARY OF THE INVENTION
[0006] In one embodiment of the present invention are disclosed
microtubule polymerization-inhibiting compounds represented by
formula (I) 2
[0007] or pharmaceutically acceptable salts or prodrugs thereof,
wherein
[0008] L.sup.1 is selected from the group consisting of
[0009] (1) --S(O).sub.2O--,
[0010] (2) --OS(O).sub.2--,
[0011] (3) --NR.sup.7SO.sub.2--, wherein R.sup.7 is selected from
the group consisting of
[0012] (a) hydrogen,
[0013] (b) hydroxy,
[0014] (c) amidinyl,
[0015] (d) a nitrogen-protecting group,
[0016] (e) alkanoyl,
[0017] (f) alkyl,
[0018] (g) alkenyl,
[0019] (h) alkynyl,
[0020] (i) cycloalkyl,
[0021] (j) cycloalkylalkyl,
[0022] (k) cycloalkenyl,
[0023] (l) cycloalkenylalkyl,
[0024] (m) aryloyl,
[0025] (n) alkoxy,
[0026] wherein (e)-(n) can be optionally substituted with one, two,
or three substituents independently selected from the group
consisting of
[0027] (i) hydroxyl,
[0028] (ii) halo,
[0029] (iii) cyano,
[0030] (iv) azido,
[0031] (v) carboxy,
[0032] (vi) amidinyl,
[0033] (vii) alkyl,
[0034] (viii) aryl,
[0035] (ix) oxo,
[0036] (x) heteroaryl,
[0037] (xi) heterocycloalkyl,
[0038] (xii) --NR.sup.cR.sup.d, wherein R.sup.c and R.sup.d are
independently selected from the group consisting of
[0039] (1') hydrogen,
[0040] (2') alkyl,
[0041] (3') aryl,
[0042] and
[0043] (4') alkoxyalkyl,
[0044] and
[0045] (xiii) -(alkylene)-NR.sup.cR.sup.d,
[0046] wherein for (x) and (xi), the heteroaryl and the
heterocycloalkyl can be optionally substituted with 1, 2, or 3
substituents independently selected from the group consisting
of
[0047] (1') alkyl,
[0048] and
[0049] (2') a nitrogen protecting group,
[0050] (o) heterocycloalkyloyl, wherein the heterocycloalkyloyl can
be optionally substituted with 1, 2, or 3 substituents
independently selected from the group consisting of
[0051] (i) alkyl,
[0052] and
[0053] (ii) a nitrogen protecting group,
[0054] and
[0055] (p) --(CH.sub.2).sub.xNR.sup.AR.sup.B, wherein x is 0-6, and
R.sup.A and R.sup.B are independently selected from the group
consisting of
[0056] (i) hydrogen,
[0057] (ii) alkyl,
[0058] (iii) alkenyl,
[0059] (iv) alkynyl,
[0060] (v) cycloalkyl,
[0061] (vi) cycloalkylalkyl,
[0062] (vii) cycloalkenyl,
[0063] and
[0064] (viii) cycloalkenylalkyl,
[0065] (4) --SO.sub.2NR.sup.7--, wherein R.sup.7 is defined
above,
[0066] (5) --S(O)CR.sup.12R.sup.13--, wherein R.sup.12 and R.sup.13
are independently selected from the group consisting of
[0067] (a) hydrogen,
[0068] (b) alkyl,
[0069] (c) alkenyl,
[0070] and
[0071] (d) alkynyl,
[0072] (6) --SO.sub.2CR.sup.12R.sup.13--,
[0073] (7) --SCR.sup.12R.sup.13--,
[0074] (8) --CR.sup.12R.sup.13S(O)--,
[0075] (9) --CR.sup.12R.sup.13SO.sub.2--,
[0076] and
[0077] (10) --CR.sup.12R.sup.13S--,
[0078] wherein (1)-(10) are shown with their left ends attached to
R.sup.1 and their right ends attached to the phenyl ring;
[0079] R.sup.1 is aryl or heteroaryl, wherein the aryl or the
heteroaryl can be optionally substituted with 1, 2, 3, 4,or 5
substituents independently selected from the group consisting
of
[0080] (a) oxo,
[0081] (b) azido,
[0082] (c) carboxy,
[0083] (d) carboxaldehyde,
[0084] (e) cyano,
[0085] (f) halo,
[0086] (g) hydroxy,
[0087] (h) nitro,
[0088] (i) perfluoroalkyl,
[0089] (j) perfluoroalkoxy,
[0090] (k) alkyl,
[0091] (l) alkenyl,
[0092] (m) alkynyl,
[0093] (n) alkanoyloxy,
[0094] (o) alkoxycarbonyl,
[0095] (p) cycloalkyl,
[0096] (q) cycloalkylalkyl,
[0097] (r) cycloalkenyl,
[0098] (s) cycloalkenylalkyl,
[0099] (t) alkanoyl,
[0100] (u) alkoxy,
[0101] (v) cycloalkoxy,
[0102] (w) aryloxy,
[0103] (x) heteroaryloxy,
[0104] (y) thioalkoxy
[0105] (z) alkylsulfinyl,
[0106] (aa) alkylsulfonyl,
[0107] (bb) --NR.sup.8R.sup.9, wherein R.sup.8 and R.sup.9 are
independently selected from the group consisting of
[0108] (i) hydrogen
[0109] (ii) alkyl,
[0110] (iii) arylalkyl,
[0111] and
[0112] (iv) alkanoyl, wherein the alkanoyl can be optionally
substituted with 1 or 2 substituents independently selected from
the group consisting of
[0113] (1') halo
[0114] (2') hydroxy,
[0115] and
[0116] (3') --NR.sup.10R.sup.11 wherein R.sup.10 and R.sup.11 are
independently hydrogen or alkyl,
[0117] and
[0118] (cc) --SO.sub.2NR.sup.8R.sup.9, wherein R.sup.8 and R.sup.9
are defined above;
[0119] R.sup.2 and R.sup.6 are independently selected from the
group consisting of
[0120] (1) hydrogen,
[0121] (2) alkyl,
[0122] (3) alkoxy,
[0123] (4) thioalkoxy;
[0124] and
[0125] (5) hydroxy,
[0126] and
[0127] R.sup.3, R.sup.4, and R.sup.5 are independently selected
from the group consisting of
[0128] (1) alkyl,
[0129] (2) alkoxy,
[0130] (3) thioalkoxy,
[0131] and
[0132] (4) hydroxy;
[0133] all of the foregoing with the proviso that combinations
wherein L.sup.1 is --NR.sup.7SO.sub.2-- and R.sup.1 is
[0134] (1) unsubstituted or substituted 1H-indoly-7-yl,
[0135] (2) phenyl which is 2-monosubstituted with
--NR.sup.8R.sup.9,
[0136] (3) pyrid-3-yl which is 2-monosubstituted with
--NR.sup.8R.sup.9,
[0137] or
[0138] (4) pyrimidin-5-yl which is 4-monosubstituted with
--NR.sup.8R.sup.9,
[0139] are excluded therefrom.
[0140] In a preferred embodiment of the invention are compounds
wherein L.sup.1 is --SO.sub.2NR.sup.7--, and R.sup.7 is defined
above.
[0141] In another preferred embodiment of the invention are
compounds wherein R.sup.1 is aryl.
[0142] In another preferred embodiment of the invention are
compounds wherein R.sup.1 optionally substituted heteroaryl,
particularly N-methyl substituted 1H-indolyl.
[0143] In another preferred embodiment of the invention are
compounds wherein R.sup.7 is substituted alkanoyl, substituted
aryloyl, or optionally substituted heterocycloalkyloyl.
[0144] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --NR.sup.7SO.sub.2--, and R.sup.7 is
defined above.
[0145] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --SO.sub.2CR.sup.12R.sup.13--.
[0146] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --SCR.sup.12R.sup.13--.
[0147] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --CR.sup.12R.sup.13S(O)--.
[0148] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --CR.sup.12R.sup.13SO.sub.2--.
[0149] In another preferred embodiment of the invention are
compounds wherein L.sup.1 is --CR.sup.12R.sup.13S--.
[0150] In yet another preferred embodiment of the invention are
compounds wherein L.sup.1 is --OSO.sub.2--.
[0151] In still yet another preferred embodiment of the invention
are compounds wherein L.sup.1 is --SO.sub.2O--.
[0152] In another embodiment of the invention are disclosed methods
of inhibiting polymerization of tubulin in a mammal in recognized
need of such treatment comprising administering an effective amount
of a compound having formula (I).
[0153] In yet another embodiment of the invention are disclosed
methods of treating cancer in a mammal in recognized need of such
treatment comprising administering an effective amount of a
compound having formula (I).
[0154] In still yet another embodiment of the invention are
disclosed pharmaceutical compositions containing compounds having
formula (I).
DETAILED DESCRIPTION OF THE INVENTION
[0155] Definition of Terms
[0156] The term "alkanoyl," as used herein, refers to an alkyl
group attached to the parent molecular group through a carbonyl
group. The alkanoyl groups of this invention can be optionally
substituted.
[0157] The term "alkanoyloxy," as used herein, refers to an
alkanoyl group attached to the parent molecular group through an
oxygen atom.
[0158] The term "alkenyl," as used herein, refers to a monovalent
straight or branched chain group of two to six carbon atoms
containing at least one carbon-carbon double bond. The alkenyl
groups of this invention can be optionally substituted.
[0159] The term "alkoxy," as used herein, refers to an alkyl group
attached to the parent molecular group through an oxygen atom. The
alkoxy groups of this invention can be optionally substituted.
[0160] The term "alkoxyalkyl," as used herein, refers to an alkoxy
group attached to the parent molecular moiety through an alkyl
group.
[0161] The term "alkoxycarbonyl," as used herein, refers to an
alkoxy group attached to the parent molecular group through a
carbonyl group.
[0162] The term "alkyl," as used herein, refers to a monovalent
group of one to six carbon atoms derived from a straight or
branched chain saturated hydrocarbon. The alkyl groups of this
invention can be optionally substituted.
[0163] The term "alkylating agent," as used herein, represents a
reagent capable of donating an alkyl group during the course of a
reaction. Examples of alkylating agents include methyl triflate,
dimethyl sulfate, iodomethane, bromobutane, bromopropane, and the
like.
[0164] The term "alkylene," as used herein, refers to a saturated
divalent hydrocarbon group derived from a straight or branched
chain saturated hydrocarbon by the removal of two hydrogen
atoms.
[0165] The term "alkylsulfinyl," as used herein, refers to an alkyl
group attached to the parent molecular group through an --S(O)--
group.
[0166] The term "alkylsulfonyl," as used herein, refers to an alkyl
group attached to the parent molecular group through an
--SO.sub.2-- group.
[0167] The term "alkynyl," as used herein, refers to a monovalent
straight or branched chain group of two to six carbon atoms
containing at least one carbon-carbon triple bond. The alkynyl
groups of this invention can be optionally substituted.
[0168] The term "amidinyl," as used herein, refers to an
--NR.sup.10R.sup.11 group, wherein R.sup.10 and R.sup.11 are
defined above, connected to the parent molecular group through an
imine.
[0169] The term "aryl," as used herein, refers to a mono- or
bicyclic-carbocyclic ring system having at least one aromatic ring.
Aryl groups are exemplified by those derived from phenyl, naphthyl,
1,2-dihydronaphthyl, 1,2,3,4-tetrahydronaphthyl, fluorenyl,
indanyl, indenyl, azulenyl, and troponyl. Bicyclic aryl groups of
this invention can be attached to the parent molecular group
through either a saturated or unsaturated part of the group. The
aryl groups of this invention can be optionally substituted.
[0170] The term "arylalkyl," as used herein, refers to an alkyl
group to which is attached at least one aryl group.
[0171] The term "aryloxy," as used herein, refers to an aryl group
attached to the parent molecular group through an oxygen atom.
[0172] The term "aryloyl," as used herein, refers to an aryl group
attached to the parent molecular moiety through a carbonyl group.
The aryloyl groups of this invention can be optionally
substituted.
[0173] The term "azido," as used herein, refers to --N.sub.3.
[0174] The term "base," as used herein, represents a reagent
capable of accepting protons during the course of a reaction.
Examples of bases include carbonates such as potassium carbonate,
potassium bicarbonate sodium carbonate, sodium bicarbonate, and
cesium carbonate; halides such as cesium fluoride; phosphates such
as potassium phosphate, potassium dihydrogen phosphate, and
potassium hydrogen phosphate; hydroxides such as lithium hydroxide,
sodium hydroxide, and potassium hydroxide; disilylamides such as
lithium hexamethyldisilazide, potassium hexamethyldisilazide, and
sodium hexamethyldisilazide; trialkylamines such as triethylamine
and diisopropylamine; heterocyclic amines such as imidazole,
pyridine, pyridazine, pyrimidine, and pyrazine; bicyclic amines
such as DBN and DBU; and hydrides such as lithium hydride, sodium
hydride, and potassium hydride. The base chosen for a particular
conversion depends on the nature of the starting materials, the
solvent or solvents in which the reaction is conducted, and the
temperature at which the reaction is conducted.
[0175] The term "carboxaldehyde," as used herein, refers to
--CHO.
[0176] The term "carbonyl," as used herein, refers to --C(O)--.
[0177] The term "carboxy," as used herein, refers to
--CO.sub.2H.
[0178] The term "cyano," as used herein, refers to --CN.
[0179] The term "cycloalkenyl," as used herein, refers to a
monovalent cyclic or bicyclic hydrocarbon of four to twelve carbon
atoms having at least one carbon-carbon double bond.
[0180] The term "cycloalkenylalkyl," as used herein, refers to an
alkyl group, as defined herein, to which is attached at lease one
cycloalkenyl group.
[0181] The term "cycloalkyl," as used herein, refers to a
monovalent saturated cyclic hydrocarbon group of three to twelve
carbon atoms.
[0182] The term "cycloalkylalkyl," as used herein, refers to an
alkyl group, as defined herein, to which is attached at lease one
cycloalkyl group.
[0183] The term "halo," as used herein, refers to --F, --Cl, --Br
or --I.
[0184] The term "heteroaryl," as used herein, refers to a cyclic
aromatic group having five or six ring atoms, wherein at least one
ring atom is selected from the group consisting of oxygen, sulfur,
and nitrogen, and the remaining ring atoms are carbon. The nitrogen
atoms can be optionally quaternized, and the sulfur atoms can be
optionally oxidized. Heteroaryl groups of this invention include
those derived from furan, imidazole, isothiazole, isoxazole,
oxadiazole, oxazole, 1,2,3-oxadiazole, pyrazine, pyrazole,
pyridazine, pyridine, pyrimidine, pyrroline, thiazole,
1,3,4-thiadiazole, thiene, triazole, and tetrazole.
[0185] The term "heteroaryl," as used herein, also includes
bicyclic or tricyclic rings, wherein any of the aforementioned
heteroaryl rings is fused to one or two rings independently
selected from the group consisting of an aryl ring, a cycloalkyl
ring, a cycloalkenyl ring, and another monocyclic heteroaryl or
heterocyaloalkyl ring. These bicyclic or tricyclic heteroaryls
include those derived from benzo[b]furan, benzo[b]thiene,
benzimidazole, cinnoline, imidazo[4,5-c]pyridine, quinazoline,
thieno[2,3-c]pyridine, thieno[3,2-b]pyridine,
thieno[2,3-b]pyridine, indolizine, imidazo[1,2-a]pyridine,
quinoline, isoquinoline, phthalazine, quinoxaline, naphthyridine,
quinolizine, indole, isoindole, indazole, indoline, benzoxazole,
benzopyrazole, benzothiozole, imidazo[1,5-a]pyridine,
pyrazolo[1,5-a]pyridine, imidazo[1,2-a]pyrimidine,
imidazo[1,2-c]pyrimidine, imidazo[1,5-a]pyrimidine,
imidazo[1,5-c]pyrimidine, pyrrolo[2,3-b]pyridine,
pyrrolo[2.3-c]pyridine, pyrrolo[3,2-c]pyridine,
pyrrolo[3,2-b]pyridine, pyrrolo[2,3-d]pyrimidine,
pyrrolo[3,2-d]pyrimidin- e, pyrrolo[2,3-b]pyrazine,
pyrazolo[1,5-a]pyridine, pyrrolo[1,2-b]pyridazine,
pyrrolo[1,2-c]pyrimidine, pyrrolo[1,2-a]pyrimidine,
pyrrolo[1,2-a]pyrazine, triazo[1,5-a]pyridine, pteridine, purine,
carbazole, acridine, phenazine, phenothiazine, phenoxazine,
1,2-dihydropyrrolo[3,2,1-hi]indole, indolizine,
imidazo[1,2-a]pyridine, imidazo[1,5-a]pyridine,
imidazo[1,2-a]pyridine, pyrido[1,2-a]indole,
10,11-dihydro-5H-dibenzo[b,e][1,4]diazepine,
5,11-dihydrodibenzo[b,e][1,4]oxazepine, and 2(1H)-pyridinone. The
bicyclic or tricyclic heteroaryl rings and can be attached to the
parent molecular group through either the heretoaryl group itself
or the aryl, cycloalkyl, cycloalkenyl, or heterocycloalkyl group to
which it is fused.
[0186] The term "heteroaryl," as used herein, also includes
compounds having formula 3
[0187] wherein W* is --O-- or --NR.sup.10--, wherein R.sup.10 is
defined above, Y* is --C(O)-- or --(C(R.sup.10)(R.sup.11)).sub.v--,
wherein R.sup.10 and R.sup.11 are defined above, and v is 1, 2, or
3, and Z* is --CH.sub.2--, --O--, --CH.sub.2S(O).sub.t--, wherein t
is zero, one or two, --CH.sub.2O--, --CH.sub.2NR.sup.10--, or
--NR.sup.10--, wherein R.sup.10 is defined above. The heteroaryl
groups of this invention can be optionally substituted.
[0188] The term "heteroaryloxy," as used herein, refers to a
heteroaryl group attached to the parent molecular group through an
oxygen atom. The heteroaryloxy groups of this invention can be
optionally substituted.
[0189] The term "heterocycloalkyl," as used herein, refers to a
non-aromatic five-, six- or seven-membered ring having between one
and three heteroatoms independently selected from oxygen, sulfur,
and nitrogen, wherein each 5-membered ring has zero to one double
bonds and each six-membered ring has zero to 2 double bonds.
Representative heterocycloalkyl groups include
3,4-dihydropyridinyl, pyrrolidinyl, piperidinyl, piperazinyl,
morpholinyl, tetrahydrofuryl, and 1,2,3,4-tetrahydropyridinyl. The
heterocycloalkyl groups of this invention can be optionally
substituted.
[0190] The term "heterocycloalkyloyl," as used herein, refers to a
heterocycloalkyl group attached to the parent molecular moiety
through a carbonyl group.
[0191] The term "hydroxy," as used herein, refers to --OH.
[0192] The term "imine," as used herein, refers to
--C(.dbd.NR.sup.21)--, wherein R.sup.21 is defined above.
[0193] The term "nitro," as used herein, refers to --NO.sub.2.
[0194] The term "nitrogen-protecting group," as used herein, refers
to groups intended to protect an amino group against undesirable
reactions during synthetic procedures. Commonly used
nitrogen-protecting groups are disclosed in Greene, "Protective
Groups In Organic Synthesis," (John Wiley & Sons, New York
(1991)). Common N-protecting groups comprise (a) acyl groups such
as formyl, acetyl, propionyl, pivaloyl, tert-butylacetyl,
2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl,
phthalyl, o-nitrophenoxyacetyl, .alpha.-chlorobutyryl, benzoyl,
4-chlorobenzoyl, 4-bromobenzoyl, and 4-nitrobenzoyl, (b) sulfonyl
groups such as benzenesulfonyl, and para-toluenesulfonyl, (c)
carbamate forming groups such as benzyloxycarbonyl,
para-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl,
p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl,
p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl,
3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl,
4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl,
3,4,5-trimethoxybenzyloxycarbonyl,
1-(p-biphenylyl)-1-methylethoxycarbony- l,
.alpha.,.alpha.-dimethyl-3,5-dimethoxybenzyloxycarbonyl,
benzhydryloxycarbonyl, tert-butyloxycarbonyl,
diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl,
methoxycarbonyl, allyloxycarbonyl, 2,2,2,-trichloroethoxycarbonyl,
phenoxycarbonyl, 4-nitrophenoxy carbonyl, cyclopentyloxycarbonyl,
adamantyloxycarbonyl, cyclohexyloxycarbonyl, and
phenylthiocarbonyl, (d) arylalkyl groups such as benzyl,
triphenylmethyl, and benzyloxymethyl, and (e) silyl groups such as
trimethylsily. Preferred N-protecting groups are formyl, acetyl,
benzoyl, pivaloyl, tert-butylacetyl, phenylsulfonyl, benzyl,
tert-butyloxycarbonyl (Boc) and benzyloxycarbonyl (Cbz).
[0195] The term "oxo," as used herein, refers to (.dbd.O).
[0196] The term "perfluoroalkyl," as used herein, refers to an
alkyl group in which all of the hydrogen atoms have been replaced
by fluorine atoms.
[0197] The term "perfluoroalkoxy," as used herein, refers to a
perfluoroalkyl group attached to the parent molecular group through
an oxygen atom.
[0198] The term "perfluoroalkyl," as used herein, refers to an
alkyl group in which all of the hydrogen atoms have been replaced
by fluoride atoms.
[0199] The term "pharmaceutically acceptable salt," as used herein,
refers to salts which are, within the scope of sound medical
judgment, suitable for use in contact with the tissues of humans
and lower animals without undue toxicity, irritation, or allergic
response and are commensurate with a reasonable benefit/risk ratio.
Pharmaceutically acceptable salts are well-known in the art. For
example, S. M. Berge, et al. describe pharmaceutically acceptable
salts in detail in J. Pharmaceutical Sciences, 1977, 66: 1 et seq,
hereby incorporated by reference. The salts may be prepared in situ
during the final isolation and purification of the compounds of the
invention or separately by reacting a free base function with a
suitable acid. Representative acid addition salts include acetate,
adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate,
bisulfate, butyrate, camphorate, camphorsufonate, digluconate,
glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate,
hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethansulfonate
(isethionate), lactate, maleate, methanesulfonate, nicotinate,
2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate,
3-phenylpropionate, picrate, pivalate, propionate, succinate,
tartrate, thiocyanate, phosphate, glutamate, bicarbonate,
p-toluenesulfonate and undecanoate. Also, the basic
nitrogen-containing groups can be quaternized with such agents as
lower alkyl halides such as methyl, ethyl, propyl, and butyl
chlorides, bromides and iodides; dialkyl sulfates such as dimethyl,
diethyl, dibutyl and diamyl sulfates; long chain halides such as
decyl, lauryl, myristyl, and stearyl chlorides, bromides and
iodides; and arylalkyl halides such as benzyl and phenethyl
bromides. Water or oil-soluble or dispersible products are thereby
obtained. Examples of acids which may be employed to form
pharmaceutically acceptable acid addition salts include such
inorganic acids as hydrochloric acid, hydrobromic acid, sulphuric
acid and phosphoric acid and such organic acids as oxalic acid,
maleic acid, succinic acid, and citric acid.
[0200] Basic addition salts can be prepared in situ during the
final isolation and purification of compounds of this invention by
reacting a carboxylic acid-containing moiety with a suitable base
such as the hydroxide, carbonate or bicarbonate of a
pharmaceutically acceptable metal cation or with ammonia or an
organic primary, secondary or tertiary amine. Pharmaceutically
acceptable salts include, but are not limited to, cations based on
alkali metals or alkaline earth metals such as lithium, sodium,
potassium, calcium, magnesium and aluminum salts and the like and
nontoxic quaternary ammonia and amine cations including ammonium,
tetramethylammonium, tetraethylammonium, methylamine,
dimethylamine, trimethylamine, triethylamine, diethylamine, and
ethylamine. Other representative organic amines useful for the
formation of base addition salts include ethylenediamine,
ethanolamine, diethanolamine, piperidine, and piperazine.
[0201] The term "pharmaceutically acceptable prodrugs," as used
herein refers to, those prodrugs of the compounds of the present
invention which are, within the scope of sound medical judgment,
suitable for use in contact with the tissues of humans and lower
animals with undue toxicity, irritation, allergic response, and the
like, commensurate with a reasonable benefit/risk ratio, and
effective for their intended use, as well as the zwitterionic
forms, where possible, of the compounds of the invention.
[0202] The term "prodrug," as used herein, represents compounds
which are rapidly transformed in vivo to parent compounds having
formula (I), for example, by hydrolysis in blood. A thorough
discussion is provided in T. Higuchi and V. Stella, Prodrugs as
Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series, and
in Edward B. Roche, ed., Bioreversible Carriers in Drug Design,
American Pharmaceutical Association and Pergamon Press, 1987, both
of which are hereby incorporated by reference. Particularly
preferred prodrugs of the invention include compounds having
formula (I), wherein a nitrogen, hydroxy, or thiol group has
attached thereto an aminoacyl, bisaminoacyl (2-mer), or
trisaminoacyl (3-mer) group optionally capped with a carboxyl
protecting group. The term "aminoacyl," as used herein, refers to a
group derived from naturally or unnaturally occuring amino acids.
Representative aminoacyl groups include those derived from glycine,
alanine, .beta.-alanine, valine, leucine, iso-leucine, methionine,
serine, threonine, cysteine, phenylalanine, and tyrosine in the
racemic, D or L configurations. The aminoacyl groups of this
invention can be optionally substituted. The terms "bisaminoacyl"
and "trisaminoacyl," as used herein, refer to di-and tri-aminoacyl
groups, respectively. Representative examples of bisaminoacyl and
trisaminoacyl groups include 2-mers and 3-mers derived from
glycine, alanine, .beta.-alanine, valine, leucine, iso-leucine,
methionine, serine, threonine, cysteine, phenylalanine, and
tyrosine in the racemic, D or L configurations.
[0203] The term "thioalkoxy," as used herein, refers to an alkyl
group attached to the parent molecular group through a sulfur
atom.
[0204] The present invention contemplates metabolites formed by in
vivo biotransformation of compounds having formula (I). The term
"metabolite," as used herein, refers to compounds formed by in vivo
biotransformation of compounds having formula (I) by oxidation,
reduction, hydrolysis, or conjugation. The present invention also
contemplates compounds which undergo in vivo biotransformation such
as by oxidation, reduction, hydrolysis, or conjugation to form
compounds having formula (I). A thorough discussion of
biotransformation is provided in Goodman and Gilman's, The
Pharmacological Basis of Therapeutics, seventh edition, hereby
incorporated by reference.
[0205] Asymmetric or chiral centers may exist in the compounds of
the present invention. The present invention contemplates the
various stereoisomers and mixtures thereof. Individual
stereoisomers of compounds of the present invention are prepared
synthetically from commercially available starting materials which
contain asymmetric or chiral centers or by preparation of mixtures
of enantiomeric compounds followed by resolution well-known to
those of ordinary skill in the art. These methods of resolution are
exemplified by (1) attachment of a racemic mixture of enantiomers
to a chiral auxiliary, separation of the resulting diastereomers by
recrystallization or chromatography and liberation of the optically
pure product from the auxiliary or (2) direct separation of the
mixture of optical enantiomers on chiral chromatographic
columns.
[0206] Geometric isomers may also exist in the compounds of the
present invention. The present invention contemplates the various
geometric isomers and mixtures thereof resulting from the
arrangement of substituents around a carbon-carbon double bond.
[0207] Compounds falling within the scope of formula (I) include,
but are not limited to
[0208] 4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0209]
3,4-dimethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0210]
4-trifluoromethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0211]
4-trifluoromethyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0212] 4-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0213] 4-amino-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0214]
4-((2-chloroacetyl)amino)-N-(3,4,5-trimethoxyphenyl)benzenesulfonam-
ide,
[0215] 2-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0216]
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0217]
3-amino-4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide,
[0218]
1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
[0219] N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
[0220]
5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
[0221]
1-methyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide,
[0222]
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide-
,
[0223]
5-amino-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide-
,
[0224]
5-amino-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide,
[0225] N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
[0226]
1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
[0227]
N,1-dimethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
[0228] 3,4,5-trimethoxy-N-(4-methoxyphenyl)benzenesulfonamide,
[0229]
N-(3-hydroxy-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
[0230]
N-(1-methyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
[0231]
N-(4-(dimethylamino)phenyl)-3,4,5-trimethoxybenzenesulfonamide,
[0232]
N-(4-fluoro-3-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
[0233]
3,4,5-trimethoxy-N-(4-(trifluoromethoxy)phenyl)benzenesulfonamide,
[0234]
3,4,5-trimethoxy-N-(2,3,4,5,6-pentafluorophenyl)benzenesulfonamide,
[0235]
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide,
[0236]
3,4,5-trimethoxy-N-(1-methyl-1H-indol-4-yl)benzenesulfonamide,
[0237]
3,4,5-trimethoxy-N-(1-methyl-1H-indol-6-yl)benzenesulfonamide,
[0238] N-(1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
[0239]
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
[0240]
N-(3-chloro-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
[0241] N-(1H-indazol-5-yl)-3,4,5-trimethoxybenzenesulfonamide,
[0242]
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-6-yl)benzenesulfonamid-
e,
[0243]
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-5-yl)benzenesulfonamid-
e,
[0244]
3,4,5-trimethoxy-N-methyl-N-(1-methyl-1H-indol-5-yl)benzenesulfonam-
ide,
[0245]
3,4,5-trimethoxy-N-(2-(dimethylamino)ethyl)-N-(1-methyl-1H-indol-5--
yl)benzenesulfonamide,
[0246] 1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate,
[0247] (3,4,5-trimethoxyphenyl) 4-methoxybenzenesulfonate,
[0248] 3,4,5-trimethoxyphenyl) 4-methylbenzenesulfonate,
[0249] 1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate,
[0250] 3,4,5-trimethoxyphenyl)
3-amino-4-methoxybenzenesulfonate,
[0251]
(3,4,5-trimethoxyphenyl)-4-(dimethylamino)benzenesulfonate,
[0252] 4-methylphenyl 3,4,5-trimethoxybenzenesulfonate,
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate, and
[0253] 4-methoxyphenyl 3,4,5-trimethoxybenzenesulfonate.
[0254] tert-butyl
2-((1-methyl-1H-indol-5-yl)((3,4,5-trimethoxyphenyl)sulf-
onyl)amino)ethylcarbamate,
[0255]
N-(2-hydroxyethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benze-
nesulfonamide,
[0256]
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3,4,5-trimethoxybenzenesulfona-
mide,
[0257]
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzene-
sulfonamide,
[0258]
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzenesulfona-
mide,
[0259]
1-ethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
[0260]
N-acetyl-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonam-
ide,
[0261] 3,4,5-trimethoxy-N-(6-quinolinyl)benzenesulfonamide,
[0262]
N-(2-hydroxyethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5--
sulfonamide,
[0263]
N-(2-fluoroethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-s-
ulfonamide,
[0264]
N-ethyl-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide-
,
[0265] 4-nitrophenyl-3,4,5-trimethoxybenzenesulfonate,
[0266] 4-aminophenyl-3,4,5-trimethoxybenzenesulfonate,
[0267] 4-dimethylaminophenyl-3,4,5-trimethoxybenzenesulfonate,
[0268] 3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate,
[0269] 1-methyl-2-oxo-1,2-dihydro-4-pyridinyl
3,4,5-trimethoxybenzenesulfo- nate.
[0270] 3,4,5-trimethoxyphenyl
3-((3-aminopropanoyl)amino)-4-methoxybenzene- sulfonate,
[0271] 3,4,5-trimethoxyphenyl
3-(((2R)-2-aminopropanoyl)amino)-4-methoxybe- nzenesulfonate,
[0272] 3,4,5-trimethoxyphenyl
3-(((2R)-2-amino-3-methylbutanoyl)amino)-4-m-
ethoxybenzenesulfonate,
[0273]
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-in-
dole-5-sulfonamide,
[0274]
1-methyl-N-(((2S)-1-methylpyrrolidinyl)carbonyl)-N-(3,4,5-trimethox-
yphenyl)-1H-indole-5-sulfonamide,
[0275]
N-((2S)-2-(dimethylamino)-3-methylbutanoyl)-1-methyl-N-(3,4,5-trime-
thoxyphenyl)-1H-indole-5-sulfonamide,
[0276]
N-((2S)-2-amino-3-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxypheny-
l)-1H-indole-5-sulfonamide,
[0277]
1-methyl-N-((2S)-2-methylamino)propanoyl)-N-(3,4,5-trimethoxyphenyl-
)-1H-indole-5-sulfonamide,
[0278]
N-((2S)-2-amino-2-phenylethanoyl)-1-methyl-N-(3,4,5-trimethoxypheny-
l)-1H-indole-5-sulfonamide,
[0279]
N-((2S)-2-amino-3-phenylpropanoyl)-1-methyl-N-(3,4,5-trimethoxyphen-
yl)-1H-indole-5-sulfonamide,
[0280]
1-methyl-N-((2S)-pyrrolidinylcarbonyl)-N-(3,4,5-trimethoxyphenyl)-1-
H-indole-5-sulfonamide,
[0281]
N-((2S)-2,6-diaminohexanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-
-indole-5-sulfonamide,
[0282]
N-((2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl)-1-methyl-N-(3,4,5-tr-
imethoxyphenyl)-1H-indole-5-sulfonamide,
[0283]
(2S)-2-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulf-
onyl)anilino)butanoic acid,
[0284]
(3S)-3-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulf-
onyl)anilino)butanoic acid,
[0285]
(2S)-2-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulf-
onyl)anilino)pentanoic acid,
[0286]
(4S)-4-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulf-
onyl)anilino)pentanoic acid,
[0287] N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl
-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide,
[0288]
1-methyl-N-(4-morpholinylacetyl)-N-(3,4,5-trimethoxyphenyl)-1H-indo-
le-5-sulfonamide,
[0289]
1-methyl-N-((4-methyl-1-piperazinyl)acetyl)-N-(3,4,5-trimethoxyphen-
yl)-1H-indole-5-sulfonamide,
[0290]
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-i-
ndole-5-sulfonamide,
[0291] 1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfanyl)benzene,
[0292] 1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfinyl)benzene,
[0293] 1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfonyl)benzene,
[0294]
1,2,3-trimethoxy-5-((1-(4-methoxyphenyl)-1-methylethyl)sulfonyl)ben-
zene,
[0295]
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline,
[0296]
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline,
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline,
[0297]
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)ani-
line,
[0298]
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfanyl)methyl)benzene,
[0299]
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfonyl)methyl)benzene,
[0300]
1,2,3-trimethoxy-5-(1-((4-methoxyphenyl)sulfonyl)-1-methylethyl)ben-
zene,
[0301] 2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline,
[0302]
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)ani-
line,
[0303] 1,2,3-trimethoxy-5-((phenylsulfonyl)methyl)benzene,
[0304]
N-(2-aminoacetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-s-
ulfonamide,
[0305]
N-(2-aminoacetyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzen-
esulfonamide,
[0306]
N-((2S)-2-aminopropanoyl]-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-in-
dole-5-sulfonamide,
[0307]
N-((2S)-2-aminopropanoyl]-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-y-
l)benzenesulfonamide,
[0308]
N-(3-aminopropanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole--
5-sulfonamide,
[0309]
N-(3-aminopropanoyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)ben-
zenesulfonamide,
[0310]
(2S)-2-amino-N-((1S)-1-methyl-2-oxo-2-(3,4,5-trimethoxy((1-methyl-1-
H-indol-5-yl)sulfonyl)anilino)ethyl)propanamide,
[0311]
(2S)-2-amino-N-((1S)-1-methyl-2-((1-methyl-1H-indol-5-yl)((3,4,5-tr-
imethoxyphenyl)sulfonyl)amino)-2-oxoethyl)propanamide,
[0312]
N-((2S)-2-amino-3-hydroxypropanoyl)-1-methyl-N-(3,4,5-trimethoxyphe-
nyl)-1H-indole-5-sulfonamide, and
[0313]
N-((2S)-2-amino-3-hydroxypropanoyl)-3,4,5-trimethoxy-N-(1-methyl-1H-
-indol-5-yl)benzenesulfonamide.
[0314] A more preferred compound for the practice of the present
invention is
[0315]
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-in-
dole-5-sulfonamide.
[0316] Determination of Biological Activity
[0317] Compounds of this invention were tested in a 48-hour
cellular proliferation assay which uses human colon adenocarcinoma,
MDR positive (HCT-15) cells, and human lung large cell carcinoma,
MDR negative (NCI-H460) cells, in the 96-well microtitre format
described in Skehan P., et al. New Colorimetric Cytotoxicity Assay
for Anticancer Drug Screening. 1990, J. Natl. Cancer Inst.
82:1107-1112, hereby incorporated by reference. Briefly, the wells
of a microtitre plate were charged sequentially with cultured cells
and compounds of the invention (1.0.times.10.sup.-4 to
1.0.times.10.sup.-11 M in 10% DMSO prepared by dissolving compounds
of the invention in DMSO and adding 11 .mu.L of the DMSO solution
to 100 .mu.L of culture medium for a final DMSO concentration of
10%). Two of the following controls were also present in each
microtitre plate: a solvent (DMSO) control without drug that
yielded a 0% inhibition level and a trichloroacetic acid-treated
well that yielded a 100% inhibition level. The cells were grown in
culture (37.degree. C., 5% CO.sub.2 atmosphere) for 48 hours then
fixed by the addition of trichloroacetic acid. The wells were
stained with sulforhodamine, washed with 1% acetic acid, and
treated with 0.01M tris buffer (100 .mu.L) to solubilize the
adherent dye. The absorbance of the dye solution was measured with
a Molecular Devices SpectraMax340 plate reader. The percent
inhibition values were obtained by calculating the proportional
response of the experimental values to the absorbance values of the
controls. The results for representative examples of compounds
having formula (I) are shown in Table 1.
1TABLE 1 Inhibitory Potency of Representative Compounds NCI-460
HCT-15 % inhibition at % inhibition at Example 10.sup.-4 M
10.sup.-4 M 1 98.5 99.4 2 99.6 >95 3 100.0 94.4 4 99.8 82.6 5
99.9 68.5 6 >95 54.8 7 89.2 96.7 8 50.0 53.8 9 47.4 77.2 10 99.6
99.8 11 74.0 79.9 12 59.3 86.2 13 41.7 15.9 14 96.1 97.6 15 45.5
50.6 16 87.0 91.9 17 <9.1 37.0 18 89.1 94.1 19 100.0 100.0 20
99.9 100.0 21 96.0 97.8 22 99.5 99.8 23 100.0 100.0 24 92.0 95.8 25
57.8 62.1 26 46.7 70.9 27 61.6 57.0 28 99.8 99.9 29 90.8 95.1 30
83.8 94.9 31 95.1 98.6 32 99.8 99.8 33 2.8 92.0 34 18.5 47.6 35
34.5 65.2 36 99.5 99.3 37 100.0 99.9 38 81.0 87.5 39 100.0 100.0 40
99.9 99.9 41 99.5 99.8 42 100.0 100.0 43 100.0 100.0 44 99.9 100.0
45 99.4 99.9 46 99.5 99.6 47 99.9 100.0 85 99.6 99.8 86 96.5 99.4
87 99.6 99.9 88 58.4 85.5 89 99.9 99.9 90 99.8 99.5 91 99.8 95.0 92
9.1 67.1 93 98.6 99.9 94 90.1 97.7 95 9.1 84.8 96 99.9 99.8
[0318] As shown by the data in Table 1, the compounds of the
invention, including, but not limited to those specified in the
examples, are useful for the treatment of disease caused or
exascerbated by cell proliferation. As cell proliferation
inhibitors, these compounds are useful in the treatment of both
primary and metastatic solid tumors and carcinomas of the breast,
colon, rectum, lung, oropharynx, hypopharynx, esophagus, stomach,
pancreas, liver, gallbladder, bile ducts, small intestine, urinary
tract including kidney, bladder and urothelium, female genital
tract including cervix, uterus, ovaries, choriocarcinoma, and
gestational trophoblastic disease, male genital tract including
prostate, seminal vesicles, testes, and germ cell tumors, endocrine
glands including thyroid, adrenal, and pituitary, skin including
hemangiomas, melanomas, sarcomas arising from bone or soft tissues
including Kaposi's sarcoma, tumors of the brain, nerves, and eyes,
meninges including astrocytomas, gliomas, glioblastomas,
retinoblastomas, neuromas, neuroblastomas, Schwannomas and
meningiomas, solid tumors arising from hematopoietic malignancies
including leukemias and chloromas, plasmacytomas, plaques, tumors
of mycosis fungoides, cutaneous T-cell lymphoma/leukemia, lymphomas
including Hodgkin's and non-Hodgkin's lymphomas, prophylaxis of
autoimmune diseases including rheumatoid, immune and degenerative
arthritis, ocular diseases including diabetic retinopathy,
retinopathy of prematurity, corneal graft rejection, retrolental
fibroplasia, neovascular glaucoma, rubeosis, retinal
neovascularization due to macular degeneration, hypoxia, abnormal
neovascularization conditions of the eye, skin diseases including
psoriasis, blood vessel diseases including hemagiomas and capillary
proliferation within atherosclerotic plaques, Osler-Webber
Syndrome, myocardial angiogenesis, plaque neovascularization,
telangiectasia, hemophiliac joints, angiofibroma, and wound
granulation.
[0319] The compounds of the present invention may also be useful
for the prevention of metastases from the tumors described above
either when used alone or in combination with radiotherapy and/or
other chemotherapeutic treatments conventionally administered to
patients for treating cancer. For example, when used in the
treatment of solid tumors, compounds of the present invention may
be administered with chemotherapeutic agents such as alpha
inteferon, COMP (cyclophosphamide, vincristine, methotrexate, and
prednisone), etoposide, mBACOD (methortrexate, bleomycin,
doxorubicin, cyclophosphamide, vincristine, and dexamethasone),
PRO-MACE/MOPP (prednisone, methotrexate (w/leucovin rescue),
doxorubicin, cyclophosphamide, paclitaxel,
etoposide/mechlorethamine, vincristine, prednisone, and
procarbazine), vincristine, vinblastine, angioinhibins, TNP-470,
pentosan polysulfate, platelet factor 4, angiostatin, LM-609,
SU-101, CM-101, Techgalan, thalidomide, SP-PG, and the like. Other
chemotherapeutic agents include alkylating agents such as nitrogen
mustards (mechloethamine, melphan, chlorambucil, cyclophosphamide
and ifosfamide), nitrosoureas including carmustine, lomustine,
semustine and streptozocin, alkyl sulfonates including busulfan,
triazines including dacarbazine, ethyenimines including thiotepa
and hexamethylmelamine, folic acid analogs including methotrexate,
pyrimidine analogues including 5-fluorouracil and cytosine
arabinoside, purine analogs including 6-mercaptopurine and
6-thioguanine, antitumor antibiotics including actinomycin D,
anthracyclines including doxorubicin, bleomycin, mitomycin C and
methramycin, hormones and hormone antagonists including tamoxifen,
cortiosteroids and miscellaneous agents including cisplatin and
brequinar. For example, a tumor may be treated conventionally with
surgery, radiation, or chemotherapy, and compounds having formula
(I), then treated with additional compound having formula (I) to
extend the dormancy of micrometastases and to stabilize and inhibit
the growth of any residual primary tumor.
[0320] Methods of Treatment
[0321] The present invention also provides pharmaceutical
compositions which comprise compounds of the present invention
formulated together with one or more non-toxic pharmaceutically
acceptable carriers. The pharmaceutical compositions may be
specially formulated for oral administration in solid or liquid
form, for parenteral injection, or for rectal administration.
[0322] The pharmaceutical compositions of this invention can be
administered to humans and other animals orally, rectally,
parenterally, intracisternally, intravaginally, intraperitoneally,
topically (as by powders, ointments, or drops), bucally, or as an
oral or nasal spray. The term "parenteral" administration as used
herein refers to modes of administration which include intravenous,
intramuscular, intraperitoneal, intrasternal, subcutaneous and
intraarticular injection and infusion.
[0323] Pharmaceutical compositions of this invention for parenteral
injection comprise pharmaceutically acceptable sterile aqueous or
nonaqueous solutions, dispersions, suspensions or emulsions as well
as sterile powders for reconstitution into sterile injectable
solutions or dispersions just prior to use. Examples of suitable
aqueous and nonaqueous carriers, diluents, solvents or vehicles
include water, ethanol, polyols (such as glycerol, propylene
glycol, polyethylene glycol, and the like), and suitable mixtures
thereof, vegetable oils (such as olive oil), and injectable organic
esters such as ethyl oleate. Proper fluidity can be maintained, for
example, by the use of coating materials such as lecithin, by the
maintenance of the required particle size in the case of
dispersions, and by the use of surfactants.
[0324] These compositions may also contain adjuvants such as
preservative, wetting agents, emulsifying agents, and dispersing
agents. Prevention of the action of microorganisms may be ensured
by the inclusion of various antibacterial and antifungal agents,
for example, paraben, chlorobutanol, phenol sorbic acid, and the
like. It may also be desirable to include isotonic agents such as
sugars, sodium chloride, and the like, Prolonged absorption of the
injectable pharmaceutical form may be brought about by the
inclusion of agents which delay absorption such as aluminum
monostearate and gelatin.
[0325] In some cases, in order to prolong the effect of the drug,
it is desirable to slow the absorption of the drug from
subcutaneous or intramuscular injection. This may be accomplished
by the use of a liquid suspension of crystalline or amorphous
material with poor water solubility. The rate of absorption of the
drug then depends upon its rate of dissolution which, in turn, may
depend upon crystal size and crystalline form. Alternatively,
delayed absorption of a parenterally administered drug form is
accomplished by dissolving or suspending the drug in an oil
vehicle.
[0326] Injectable depot forms are made by forming microencapsule
matrices of the drug in biodegradable polymers such as
polylactide-polyglycolide. Depending upon the ratio of drug to
polymer and the nature of the particular polymer employed, the rate
of drug release can be controlled. Examples of other biodegradable
polymers include poly(orthoesters) and poly(anhydrides) Depot
injectable formulations are also prepared by entrapping the drug in
liposomes or microemulsions which are compatible with body
tissues.
[0327] The injectable formulations can be sterilized, for example,
by filtration through a bacterial-retaining filter, or by
incorporating sterilizing agents in the form of sterile solid
compositions which can be dissolved or dispersed in sterile water
or other sterile injectable medium just prior to use.
[0328] Solid dosage forms for oral administration include capsules,
tablets, pills, powders, and granules. In such solid dosage forms,
the active compound is mixed with at least one inert,
pharmaceutically acceptable excipient or carrier such as sodium
citrate or dicalcium phosphate and/or a) fillers or extenders such
as starches, lactose, sucrose, glucose, mannitol, and silicic acid,
b) binders such as, for example, carboxymethylcellulose, alginates,
gelatin, polyvinylpyrrolidone, sucrose, and acacia, c) humectants
such as glycerol, d) disintegrating agents such as agar-agar,
calcium carbonate, potato or tapioca starch, alginic acid, certain
silicates, and sodium carbonate, e) solution retarding agents such
as paraffin, f) absorption accelerators such as quaternary ammonium
compounds, g) wetting agents such as, for example, cetyl alcohol
and glycerol monostearate, h) absorbents such as kaolin and
bentonite clay, and i) lubricants such as talc, calcium stearate,
magnesium stearate, solid polyethylene glycols, sodium lauryl
sulfate, and mixtures thereof. In the case of capsules, tablets and
pills, the dosage form may also comprise buffering agents.
[0329] Solid compositions of a similar type may also be employed as
fillers in soft and hard-filled gelatin capsules using such
excipients as lactose or milk sugar as well as high molecular
weight polyethylene glycols and the like.
[0330] The solid dosage forms of tablets, dragees, capsules, pills,
and granules can be prepared with coatings and shells such as
enteric coatings and other coatings well known in the
pharmaceutical formulating art. They may optionally contain
opacifying agents and can also be of a composition that they
release the active ingredient(s) only, or preferentially, in a
certain part of the intestinal tract, optionally, in a delayed
manner. Examples of embedding compositions which can be used
include polymeric substances and waxes.
[0331] The active compounds can also be in micro-encapsulated form,
if appropriate, with one or more of the above-mentioned
excipients.
[0332] Liquid dosage forms for oral administration include
pharmaceutically acceptable emulsions, solutions, suspensions,
syrups and elixirs. In addition to the active compounds, the liquid
dosage forms may contain inert diluents commonly used in the art
such as, for example, water or other solvents, solubilizing agents
and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl
carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate,
propylene glycol, 1,3-butylene glycol, dimethyl formamide, oils (in
particular, cottonseed, groundnut, corn, germ, olive, castor, and
sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene
glycols and fatty acid esters of sorbitan, and mixtures
thereof.
[0333] Besides inert diluents, the oral compositions can also
include adjuvants such as wetting agents, emulsifying and
suspending agents, sweetening, flavoring, and perfuming agents.
[0334] Suspensions, in addition to the active compounds, may
contain suspending agents as, for example, ethoxylated isostearyl
alcohols, polyoxyethylene sorbitol and sorbitan esters,
microcrystalline cellulose, aluminum metahydroxide, bentonite,
agar-agar, and tragacanth, and mixtures thereof.
[0335] Compositions for rectal or vaginal administration are
preferably suppositories which can be prepared by mixing the
compounds of this invention with suitable non-irritating excipients
or carriers such as cocoa butter, polyethylene glycol or a
suppository wax which are solid at room temperature but liquid at
body temperature and therefore melt in the rectum or vaginal cavity
and release the active compound.
[0336] Compounds of the present invention can also be administered
in the form of liposomes. As is known in the art, liposomes are
generally derived from phospholipids or other lipid substances.
Liposomes are formed by mono-or multi-lamellar hydrated liquid
crystals that are dispersed in an aqueous medium. Any non-toxic,
physiologically acceptable and metabolizable lipid capable of
forming liposomes can be used. The compositions in liposome form
can contain, in addition to a compound of the present invention,
stabilizers, preservatives, excipients, and the like. The preferred
lipids are the phospholipids and the phosphatidyl cholines
(lecithins), both natural and synthetic.
[0337] Methods to form liposomes are known in the art. See, for
example, Prescott, Ed., Methods in Cell Biology, Volume XIV,
Academic Press, New York, N.Y. (1976), p. 33 et seq.
[0338] Dosage forms for topical administration of a compound of
this invention include powders, sprays, ointments and inhalants.
The active compound is mixed under sterile conditions with a
pharmaceutically acceptable carrier and any needed preservatives,
buffers, or propellants which may be required. Opthalmic
formulations, eye ointments, powders and solutions are also
contemplated as being within the scope of this invention. Actual
dosage levels of active ingredients in the pharmaceutical
compositions of this invention may be varied so as to obtain an
amount of the active compound(s) that is effective to achieve the
desired therapeutic response for a particular patient,
compositions, and mode of administration. The selected dosage level
will depend upon the activity of the particular compound, the route
of administration, the severity of the condition being treated, and
the condition and prior medical history of the patient being
treated. However, it is within the skill of the art to start doses
of the compound at levels lower than required for to achieve the
desired therapeutic effect and to gradually increase the dosage
until the desired effect is achieved.
[0339] Generally dosage levels of about 1 to about 50, more
preferably of about 5 to about 20 mg of active compound per
kilogram of body weight per day are administered orally to a
mammalian patient. If desired, the effective daily dose may be
divided into multiple doses for purposes of administration, e.g.
two to four separate doses per day.
[0340] Synthetic Methods
[0341] The compounds and processes of the present invention will be
better understood in connection with the following synthetic
schemes, which illustrate methods by which the compounds of the
invention may be prepared. The compounds having formula (I) may be
prepared by a variety of synthetic routes. Representative
procedures are shown in Scheme 1. The groups R.sup.1, R.sup.2,
R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.12, R.sup.13, and
L.sup.1, are as previously defined unless otherwise noted. It will
be readily apparent to one of ordinary skill in the art that other
compounds within formula (I) can be synthesized by substitution of
the appropriate reactants and agents in the syntheses shown below.
It will further be apparent to one skilled in the art that the
selective protection and deprotection steps, as well as order of
the steps themselves, can be carried out in varying order,
depending on the nature of groups R.sup.1, R.sup.2, R.sup.3,
R.sup.4, R.sup.5, R.sup.6, R.sup.12, R.sup.13, and L.sup.1, to
successfully complete the syntheses of compounds having formula
(I). Commonly used protecting groups are disclosed in Greene,
"Protective Groups In Organic Synthesis," John Wiley & Sons,
New York (1981), hereby incorporated by reference. It will still
further be apparent to one of ordinary skill in the art that the
substituents R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6,
R.sup.12, R.sup.13, and L.sup.1, can be determined by selection of
the appropriate commercially available or known starting materials
or introduced synthetically by known chemical methods such as those
disclosed in Larock, "Comprehensive Organic Transformations. A
Guide to Functional Group Preparations," VCH Publishers, New York
(1989), hereby incorporated by reference.
[0342] Abbreviations
[0343] Abbreviations used in the descriptions of the schemes the
examples are: THF for tetrahydrofuran; DMF for
N,N-dimethylformamide; DMSO for dimethylsulfoxide; DEAD for diethyl
azodicarboxylate; DIAD for diisopropyl azodicarboxylate; EDC for
1-(3-dimethylaminopropyl)-3-ethylca- rbodiimide hydrochloride; LDA
for lithium diisopropylamide; TFA for trifluoroacetic acid; DMSO
for dimethylsulfoxide; DMAP for 4-(N,N-dimethylamino)pyridine; HATU
for O-(azabenzotriazole-1-yl)-N,N,N',-
N'-tetramethyluroniumhexafluorophosphate; Boc for
tert-butylcarbonyloxy; DPPA for diphenylphosphoryl azide; DCC for
dicyclohexylcarbodiimide; HOOBT for
3-hydroxy-1,2,3-benzotriazin-4(3H)-one; HOBT for
1-hydroxybenzotriazole hydrate; EDCI for
1-(3-dimethylaminopropyl)-3-ethy- lcarbodiimide hydrochloride; CDI
for 1,1'-carbonyldiimidazole; and DMAP for
N,N-dimethylaminopyridine. 4
[0344] As shown in Scheme 1, the compounds having formula (I) were
prepared by reacting intermediate (i) with intermediate (ii),
wherein X.sup.1 and X.sup.2 together are L.sup.1. With either (i)
or (ii), X.sup.1 or X.sup.2 can be any conventional activated
sulfonic acid, examples of which include sulfonyl halides, sulfonic
acid anhydrides, and N-sulfonylimidazolides, preferably sulfonyl
halides. Although the solvent used in the coupling reactions is not
particularly limited, a solvent in which the starting materials are
both soluble and which is little reactive with the materials is
preferably used. Examples of such solvents are pyridine,
triethylamine, THF, dioxane, benzene, toluene, diethyl ether,
dichloromethane, DMF, DMSO, or mixtures thereof. When an acid is
liberated with the progress of a reaction, such as when using a
halide derivative of a sulfonic acid and an amine or alcohol, it is
preferable that the reaction is carried out in the presence of a
suitable deacidifying agent. For this reason, the use of a basic
solvent such as pyridine or triethylamine is particularly
preferred, although the reaction can be run in any of the
aforementioned solvents with at least a stoichiometric amount of
basic solvent present. Although the reactions generally proceed at
room temperature, they can be run at lower or elevated
temperatures, as needed. The reaction time is generally 30 minutes
to 18 hours and can be arbitrarily selected depending on the types
of starting materials and reaction temperature. When the product
has a protected amino or hydroxyl group, the product, if necessary,
can be converted to a compound having formula (I) having a free
amino or hydroxyl group by a conventional deprotection method such
as treatment with acid, piperidine, or catalytic hydrogenation in
the presence of a catalyst such as palladium on carbon. When the
compound having formula (D has a nitro group, the nitro group can
also be reduced. Although the reduction can be conducted by any
conventional process, the conversion of a nitro group to an amine
is preferably conducted by catalytic hydrogenation using palladium
on carbon or platinum oxide as the catalyst or reduction using an
acid together with zinc, iron, or tin. The catalytic reduction is
conducted in an organic solvent such as methanol, ethanol, or THF
under normal or elevated temperature. Groups on the compounds
having formula (I) having endogenous or exogenous amino groups can
optionally alkylated, formylated, acetylated or otherwise reacted
with any number of amine-derivatization reagents well-known to
those of ordinary skill in the art. For example, acidic N--H groups
can be reacted with alcohols under Mitsunobu conditions. Preferable
Mitsunobu conditions include reacting the compounds having formula
(I) with alcohols in the presence of a phosphine, preferably
triphenylphosphine or tri n-butylphosphine and an activating agent
such as DEAD or DIAD. Although the solvent to be used in the
reaction is not particularly limited, polar, aprotic solvents such
as THF or dioxane are particularly preferable for Mitsunobu
reactions. The compounds having formula (I) can also be alkylated
with any number of reagents well-known to those of ordinary skill
in the art. For example, compounds having formula (I) can be
reacted with an unsubstituted or substituted alkylating agent in
the presence of a non-nucleophilic base such as sodium or potassium
hydride or lithium, sodium, or potassium bis(trimethylsilyl)amide.
Although the solvent to be used in the reaction is not particularly
limited, polar, aprotic solvents such as THF, DMF, DMSO, or dioxane
are particularly preferable for alkylation reactions. Compounds
having formula (I) can be reacted with halogenation agents.
Examples of halogenating agents include N-chlorosuccinamide,
N-bromosuccinamide, 1,3-bibromo-5,5-dimethylhydantoi- n,
N-bromoacetamide, bromine, chlorine, or iodine. Although the
solvent to be used in the reaction is not particularly limited,
chloroalkanes such as dichloromethane, chloroform, or carbon
tetrachloride, halogenated aromatic rings such as chlorobenzene and
dichlorobenzene, water, or organic acids, such as acetic acid, are
particularly preferable. 5
[0345] As shown in Scheme 2, compounds of formula (I) (R.sup.7 is
H) can be intraconverted to compounds of formula (I) (R.sup.7is an
aminoacyl, bisaminoacyl (2-mer), or trisaminoacyl (3-mer) residue
optionally capped with a carboxyl protecting group) by reaction
with naturally or unnaturally occurring amino acids or with 2-mers
and 3-mers derived from amino acids. Representative amino acids
include N,N-dimethylglycine, N-methyl-L-proline,
N,N-dimethyl-L-valine, N-tert-butoxycarbonyl)-L-valin- e,
N-(tert-butoxy-carbonyl)-L-N-methylalanine,
(S)-N-(tert-butoxycarbonyl)- -2-phenylglycine,
N-(tert-butoxycarbonyl)-L-phenylalanine,
N-(tert-butoxycarbonyl)-L-proline,
N,N-di-(tert-butoxycarbonyl)-L-lysine,
N-(tert-butoxycarbonyl)-L-valine,
N-(tert-butoxycarbonyl)-L-aspartic acid 1-tert-butyl ester,
N-(tert-butoxycarbonyl)-L-aspartic acid 4-tert-butyl ester,
N-(tert-butoxycarbonyl)-L-glutamic acid 1-tert-butyl ester,
N-(tert-butoxycarbonyl)-L-glutamic acid 5-tert-butyl ester,
(bis(2-methoxyethyl)amino)acetic acid, 4-morpholinylacetic acid,
(4-methyl-1-piperazinyl)acetic acid, and
4-(((tert-butoxy-carbonyl)amino)- methyl)benzoic acid in the
presence of base and an activating agent. Naturally occurring amino
acids can be purchased commercially, while unnaturally occurring
amino acids can be synthesized by methods well-known in the art.
Representative bases include 4-pyrrolidinylpyridine, DMAP, and
triethylamine. Examples of activating used in these reactions
include DCC, EDCI, HOBT, and CDI. Typical solvents used in these
reactions include dichloromethane, carbon tetrachloride, and
chloroform. The reaction temperature is about 0.degree. C. to about
30.degree. C. and depends on the method chosen. Reaction times are
typically about 2 to about 24 hours. In a preferred embodiment,
compounds of formula (D (R is H) in dichloromethane at room
temperature are reacted with a naturally or unnaturally occurring
amino acid in the presence of DCC and 4-pyrrolidinylpyridine for 16
hours to provide compounds of formula (I) (R.sup.7 is an aminoacyl,
bisaminoacyl (2-mer), or trisaminoacyl (3-mer) residue optionally
capped with a carboxyl protecting group). 6
[0346] Scheme 3 shows the method of preparation for compounds of
formula (I) (L.sup.1 is --S(O)CR.sup.12R.sup.13--,
--SO.sub.2CR.sup.12R.sup.13--, --SCR.sup.12R.sup.13--,
--CR.sup.12R.sup.13S(O)--, --CR.sup.12R.sup.13SO.sub.2--, or
--CR.sup.12R.sup.13S--). Intermediates (iii) and (iv) (one of
A.sup.1 and A.sup.2 is --CH.sub.2Cl; the other is SH) can be
combined in the presence of base to provide the desired products.
Examples of bases used in these reactions include KOH, NaOH, and
LiOH. Representative solvents include N,N-dimethylformamide,
dioxane, N-methylpyrrolidinone, and mixtures thereof. The reaction
temperature is about 25.degree. C. to about 50.degree. C. and
reaction times are typically about 1 to about 12 hours.
[0347] Compounds of formula (I) (L.sup.1 is --SCR.sup.12R.sup.13--
or --CR.sup.12R.sup.13S--) can be intraconverted to compounds of
formula (I) (L.sup.1 is S(O)CR.sup.12R.sup.13--,
--SO.sub.2CR.sup.12R.sup.13--, --SCR.sup.12R.sup.13--,
--CR.sup.12R.sup.13S(O)--, or --CR.sup.12R.sup.13SO.sub.2--) by
treatment with an oxidizing agent. Representative oxidizing agents
include H.sub.2O.sub.2 with acetic anhydride, and potassium
peroxymonosulfate (OXONE.RTM.). Examples of solvents used in these
reactions include dichloromethane, acetone, 1,2-dichloroethane,
chloroform, and mixtures thereof. The reaction temperature is about
25.degree. C. to about 40.degree. C. and depends on the method
chosen. Reaction times are typically about 8 hours to about 24
hours.
[0348] Compounds of formula (I) wherein L.sup.1 is
--SO.sub.2CR.sup.12R.su- p.13-- or --CR.sup.12R.sup.13SO.sub.2--
(R.sup.12 and R.sup.13 are hydrogen) can be intraconverted to
compounds of formula (I) wherein L.sup.1 is
--SO.sub.2CR.sup.12R.sup.13-- or --CR.sup.12R.sup.13SO.sub.2--
(R.sup.12 and R.sup.13 are alkyl) by treatment with a base and an
alkylating agent. Representative bases include lithium
hexamethyldisilazide, sodium hexamethyldisilazide, potassium
hexamethyldisilazide, and lithium diisopropylamide. Representative
alkylating agents include iodomethane, bromopropane, iodobutane,
and the like. Examples of solvents used in these reactions include
tetrahydrofuran, dioxane, diethyl ether, and methyl tert butyl
ether. Reaction times are about 2 hours to about 6 hours, and
reaction temperatures are typically about 0.degree. C. to about
30.degree. C.
[0349] The compounds and processes of the present invention will be
better understood in connection with the following examples, which
are intended as an illustration of, and not a limitation upon, the
scope of the invention as defined in the appended claims.
EXAMPLE 1
4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0350] A solution of 3,4,5-trimethoxyaniline (500 mg, 2.8 mmol) in
pyridine (5 mL) was treated with 4-methoxybenzenesulfonyl chloride
(564 mg, 2.8 mmol) in THF (5 mL), stirred at room temperature for
18 hours, concentrated, redissolved in THF (1 mL), treated with
water with stirring, and filtered to provide 820 mg of the desired
product.
[0351] MS (DCI/NH.sub.3) m/z 354 (M+H).sup.+ and 371
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.85
(d, J=7.5 Hz, 2H), 6.91 (d, J=7.5 Hz, 2H), 6.29 (s, 2H), 3.84 (s,
3H), 3.78 (s, 3H), 3.75 (s, 6H).
EXAMPLE 2
3,4-dimethoxy-N-(3,4,5-trimethoxyphenyl)benzamide
[0352] 3,4,5-trimethoxyaniline (232 mg) was processed as described
in Example 1 (substituting 3,4-dimethoxybenzenesulfonyl chloride
for 4-methoxybenzenesulfonyl chloride with) to provide 375 mg of
the desired product.
[0353] MS (DCI/NH.sub.3) m/z 384 (M+H).sup.+ and 401
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.38
(dd, J=2.4, 8.4 Hz, 1H), 7.18 (d, J=2.4 Hz, 1H), 6.87 (d, J=8.4 Hz,
1H), 6.30 (s, 2H), 3.92 (s, 3H), 3.82 (s, 3H), 3.79 (s, 3H), 3.76
(s, 6H).
EXAMPLE 3
4-trifluoromethoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0354] 3,4,5-trimethoxyaniline (200 mg) was processed as described
in Example 1 (substituting 4-trifluoromethoxybenzenesulfonyl
chloride for 4-methoxybenzenesulfonyl chloride with) to provide 330
mg of the desired product.
[0355] MS (DCI/NH.sub.3) m/z 408 (M+H).sup.+ and 425
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.05 (s, 1H), 7.08 (d, J=9.3 Hz, 1H), 6.98 (s, 2H), 6.86 (d, J=9.3
Hz, 1H), 3.76 (s, 6H), 3.70 (s, 3H), 3.64 (s, 3H).
EXAMPLE 4
4-trifluoromethyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0356] 3,4,5-trimethoxyaniline (200 mg) was processed as described
in Example 1 (substituting 4-trifluoromethylbenzenesulfonyl
chloride for 4-methoxybenzenesulfonyl chloride with) to provide 375
mg of the desired product.
[0357] MS (DCI/NH.sub.3) m/z 392 (M+H).sup.+ and 409
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.35 (s, 1H), 7.98 (s, 4H), 6.37 (s, 2H), 3.65 (s, 6H), 3.57 (s,
3H).
EXAMPLE 5
4-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0358] 3,4,5-trimethoxyaniline (500 mg) was processed as described
in Example 1 (substituting 4-nitrobenzenesulfonyl chloride for
4-methoxybenzenesulfonyl chloride) to provide 850 mg of the desired
product.
[0359] MS (DCI/NH.sub.3) m/z 369 (M+H).sup.+ and 386
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.46 (s, 1H), 8.39 (d, J=8.4 Hz, 2H), 8.02 (d, J=8.4 Hz, 2H), 6.39
(s, 2H), 3.66 (s, 6H), 3.56 (s, 3H).
EXAMPLE 6
4-amino-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0360] A solution of Example 5 (100 mg, 0.20 mmol) in in 1:1
THF:methanol (2 mL) was purged with nitrogen, treated with and 10%
palladium on carbon (100 mg), stirred under hydrogen (1 atm) for 2
hours, filtered through diatomaceous earth (Celite.RTM.), and
concentrated to provide 90 mg of the desired product. MS
(DCI/NH.sub.3) m/z 339 (M+H).sup.+ and 356 (M+NH.sub.4).sup.+;
.sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta. 9.69 (s, 1H), 7.41 (d,
J=8.7 Hz, 2H), 6.54 (d, J=8.7 Hz, 2H), 6.36 (s, 2H), 5.98 (br s,
2H), 3.64 (s, 6H), 3.55 (s, 3H).
EXAMPLE 7
4-((2-chloroacetyl)amino)-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0361] A suspension of Example 6 (30 mg, 0.09 mmole) in
dichloromethane (1 mL) was treated sequentially with triethylamine
(19 .mu.L) and chloroacetic anhydride (46 mg) to form a clear
solution, and concentrated to provide diacylated product. The
diacylated product was dissolved in methanol (1 mL), treated with
NaHCO.sub.3, and stirred for 18 hours. The slurry was washed with
water, dried (Na.sub.2SO.sub.4), filtered, and concentrated to
provide 15 mg of the desired product.
[0362] MS (DCI/NH.sub.3) m/z 415 (M+H).sup.+ and 437 (M+Na).sup.+;
.sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta. 10.67 (s, 1H), 7.74 (s,
4H), 6.36 (s, 2H), 4.28 (s, 2H), 3.64 (s, 6H), 3.55 (s, 3H).
EXAMPLE 8
2-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0363] 3,4,5-trimethoxyaniline (413 mg) was processed as described
in Example 1 (substituting 2-nitrobenzenesulfonyl chloride for
4-methoxybenzenesulfonyl chloride) to provide 800 mg of the desired
product.
[0364] MS (DCI/NH.sub.3) m/z 369 (M+H).sup.+ and 386
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
7.95 (m, 2H), 7.50 (m, 2H), 6.41 (s, 2H), 3.67 (s, 6H), 3.57 (s,
3H).
EXAMPLE 9
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
EXAMPLE 9A
4-methoxy-3-nitrobenzenesulfonyl chloride
[0365] A solution of 4-methoxy sulfonyl chloride (2 g) in sulfuric
acid (8 mL) at 0.degree. C. was treated dropwise with nitric acid,
stirred at 0.degree. C. for about 10 minutes, and carefully poured
into a separatory funnel containing diethyl ether and ice. The
water layer was extracted with diethyl ether (3--), and the
combined extracts were dried (Na2SO.sub.4), filtered, and
concentrated. The concentrate was dissolved into dichloromethane,
washed with brine, dried (Na.sub.2SO.sub.4), filtered,
concentrated, placed under high vacuum, and purified by flash
column chromatography on silica gel with 4:1 hexane/ethyl acetate
to provide the desired product.
EXAMPLE 9B
4-methoxy-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0366] 3,4,5-trimethoxyaniline (146 mg) was processed as described
in example 1 (substituting Example 9A for 4-methoxybenzenesulfonyl
chloride) to provide 280 mg of the desired product.
[0367] MS (DCI/NH.sub.3) m/z 416 (M+NH.sub.4).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 8.26 (d, J=2.1 Hz, 1H), 7.99 (dd,
J=2.1, 9 Hz, 1H), 7.53 (d, J=9 Hz, 1H), 6.39 (s, 2H), 3.98 (s, 3H),
3.67 (s, 6H), 3.57 (s, 3H).
EXAMPLE 10
3-amino-4-methoxy-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
hydrochloride
[0368] A solution of Example 9B (150 mg, 0.38 mmol) in 1:1
THF:methanol (8 mL) was treated with 10% palladium on carbon (100
mg), stirred under hydrogen (1 atm) for 1 hour, filtered through
diatomaceous earth (Celite.RTM.), and concentrated. The concentrate
was dissolved in methanol (0.5 mL), treated with 1M HCl in diethyl
ether to form a white solid, treated with additional diethyl ether
to cause the salt to fully precipitate, and filtered. The
hydroscopic salt was dissolved in water (2 mL) and lyophilized to
provide 100 mg of the desired product.
[0369] MS (DCI/NH.sub.3) m/z 369 (M+H).sup.+ 386
(M+NH.sub.4).sup.+;
[0370] 1H NMR (DMSO-d.sub.6, 300 MHz) .delta. 9.85 (s, 1H), 7.05
(d, J=2.4 Hz, 1H), 6.98 (dd, J=2.4, 8.1 Hz, 1H), 6.88 (d, J=8.1 Hz,
1H), 6.38 (s, 2H), 5.19 (s, 1H), 3.80 (s, 3H), 3.65 (s, 6H), 3.55
(s, 3H).
EXAMPLE 11
1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide
EXAMPLE 11A
N-formylindoline
[0371] A solution of indoline (5.0 g) and 98% formic acid (3.0 g)
in toluene (17 mL) was heated at reflux for 6 hours with a
Dean-Stark trap, cooled, washed with water and concentrated. The
resulting dark brown solid was dissolved in methanol, concentrated
to a fraction of its original volume, treated with 1:1 diethyl
ether/hexane, and concentrated dryness to provide 5.5 g of the
desired product.
EXAMPLE 11B
5-chlorosulfonylindoline-1-carboxaldehyde
[0372] Chlorosulfonic acid (4.6 mL) at 0.degree. C. was treated
portionwise with a sample of example 11A (2.0 g) over 30 minutes,
stirred for 5 minutes at 0.degree. C., heated at 100.degree. C.
until all bubbling ceased, carefully poured over ice and water,
stirred vigorously for 2 hours, filtered, and dried overnight in a
vacuum oven to provide 2.5 g of the desired product.
EXAMPLE 11C
25 1-formyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide
[0373] 3,4,5-trimethoxyaniline (2.51 g) was processed as described
in example 1 (substituting Example 11B for 4-methoxybenzenesulfonyl
chloride) and purified by column chromatography on silica gel with
2% methanol/dichloromethane to provide 4.8 g of the desired
product.
[0374] MS (DCI/NH.sub.3) 410 (M+NH.sub.4).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 9.85 (s, 1H), 7.05 (d, J=2.4 Hz,
1H), 6.98 (dd, J=2.4, 8.1 Hz, 1H), 6.88 (d, J=8.1 Hz, 1H), 6.38 (s,
2H), 5.19 (s, 1H), 3.80 (s, 3H), 3.65 (s, 6H), 3.55 (s, 3H).
EXAMPLE 12
N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide
[0375] A solution of Example 11C (4.8 g) in methanol (60 mL) at
room temperature was treated with HCl gas for about 8 minutes,
concentrated to dryness, and treated with ethyl acetate and water.
The organic layer was washed with saturated aqueous NaHCO.sub.3
until the aqueous washings were slightly basic, dried
(Na.sub.2SO.sub.4), filtered, and concentrated to provide 4.0 g of
the desired product.
[0376] MS (DCI/NH.sub.3) m/z 365 (M+H).sup.+ 382
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
9.69 (s, 1H), 7.34 (m, 1H), 6.42 (m, 2H), 6.37 (s, 3H), 3.65 (s,
6H), 3.53 (s, 3H), 3.50 (t, J=9 Hz, 2H), 2.94 (t, J=9 Hz, 2H).
EXAMPLE 13
5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide
EXAMPLE 13A
5-nitro-1H-indole-3-sulfonyl chloride
[0377] A solution of chlorosulfonic acid (3 mL, 45 mmol) and
Na.sub.2SO.sub.4 (700 mg, 4.9 mmol) in dichloromethane (30 mL) was
treated dropwise with a solution of 5-nitroindole (800 mg, 4.9
mmol) in dichloromethane (20 mL) over 1 hour, stirred for another
30 minutes, and decanted to provide a thick brown oil. The oil was
slowly treated with water (20 mL), stirred for 10 minutes,
filtered, and dried in a vacuum oven to provide 651 mg of the
desired product.
EXAMPLE 13B
5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide
[0378] 3,4,5-trimethoxyaniline (100 mg) was processed as described
in Example 1 (substituting Example 13A for 4-methoxybenzenesulfonyl
chloride) and purified by column chromatography on silica gel with
2% methanol/dichloromethane to provide 120 mg of the desired
product.
[0379] MS (DCI/NH.sub.3) m/z 407 (M+H).sup.+ 425
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.15 (s, 1H), 8.71 (d, J=2.1 Hz, 1H), 8.27 (s, 1H), 8.10 (dd,
J=2.1, 9 Hz, 1H), 7.66 (d, J=9 Hz, 1H), 6.38 (s, 2H), 3.60 (s, 6H),
3.50 (s, 3H).
EXAMPLE 14
1-methyl-N-(3,4,5-trimethoxyphenyl)indoline-5-sulfonamide
[0380] A solution of Example 11C (300 mg, 0.77 mmol) in THF (15 mL)
at room temperature was treated with 1M LiAlH.sub.4 (7.7 mL, 7.7
mmol) to form a solid which later dissolved to give a cloudy yellow
solution, stirred overnight at room temperature, cooled to
0.degree. C., treated sequentially with water (0.3 mL), 15% NaOH
(0.3 mL) and water (0.9 mL), stirred 30 minutes, and filtered to
remove the aluminum complex. The layers comprising the filtrate
were separated, and the organic layer was treated with ethyl
acetate (50 mL), washed with water (2.times.20 mL), dried
(Na.sub.2SO.sub.4), filtered and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 2%
methanol:dichloromethane to provide 260 mg of the desired
product.
[0381] MS (DCI/NH.sub.3) m/z 379 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 9.76 (s, 1H), 7.45 (dd, J=2.1, 8.4
Hz, 1H), 7.34 (d, 1H), 6.46 (d, J=8.4 Hz, 1H), 6.37 (s, 2H), 3.65
(s, 6H), 3.55 (s, 3H), 3.42 (t, J=8.4 Hz, 2H) 2.92 (t, J=8.4 Hz,
2H) 2.76 (s, 3H).
EXAMPLE 15
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide
EXAMPLE 15A
1-methyl-5-nitro-1H-indole-3-sulfonyl chloride
[0382] A solution of chlorosulfonic acid (1.9 mL, 28 mmol) and
Na.sub.2SO.sub.4 (403 mg, 2.8 mmol) in dichloromethane (17 mL) was
treated with a solution of 1-methyl-5-nitro-1H-indole (500 mg, 2.8
mmol) in dichloromethane (11 mL) over 1 hour, stirred for 30
minutes, and decanted to provide a thick brown oil. The oil was
slowly treated with water (20 mL), stirred for 10 minutes, and
filtered. The filtrate was dried in a vacuum oven to provide 80 mg
of the desired compound which was used in the next step without
further purification.
EXAMPLE 15B
1-methyl-5-nitro-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide
[0383] 3,4,5-trimethoxyaniline (47 mg) was processed as described
in example 1 (substituting Example 15A for 4-methoxybenzenesulfonyl
chloride) to provide 59 mg of the desired product.
[0384] MS (DCI/NH.sub.3) m/z 439 (M+NH.sub.4).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 10.20 (s, 1H), 8.71 (d, J=2.1 Hz,
1H), 8.34 (s, 1H), 8.15 (dd, J=2.1, 9 Hz, 1H), 7.76 (d, J=9 Hz,
1H), 6.40 (s, 2H), 3.91 (s, 3H), 3.61 (s, 6H) 3.51 (s, 3H).
EXAMPLE 16
5-amino-1-methyl-N-(3,4
5-trimethoxyphenyl)-1H-indole-3-sulfonamide
[0385] A solution of Example 15 (50 mg, 0.12 mmole) in 1:1
methanol:THF (2 mL) was treated with 10% palladium on carbon,
stirred under hydrogen (1 atm) for 2.5 hours, filtered through
diatomaceous earth (Celite.RTM.), concentrated, redissolved in a
small amount of methanol, treated with several drops of 1M HCl in
diethyl ether until the solution became cloudy and acidic, treated
with additional diethyl ether until a solid precipitated, filtered
and dried in a vacuum oven to provide 35 mg of the desired the
product.
[0386] MS (DCI/NH.sub.3) m/z 392 (M+H).sup.+ 409
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
9.88 (s, 1H), 7.82 (s, 1H), 7.20 (d, J=9 Hz, 1H), 6.98 (d, J=2.4
Hz, 1H), 6.64 (dd, J=2.4, 9 Hz, 1H), 6.39 (s, 2H), 3.71 (s, 3H),
3.62 (s, 6H), 3.52 (s, 3H).
EXAMPLE 17
5-amino-N-(3,4,5-trimethoxyphenyl)-1H-indole-3-sulfonamide
[0387] Example 13B (100 mg) was processed as described in Example
16 to provide 80 mg of the desired product.
[0388] MS (DCI/NH.sub.3) m/z 378 (M+H).sup.+ 395
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 8.10
(d, 1H), 7.83 (m, 1H), 7.60 (d, 1H), 7.26 (m, 1H), 6.39 (s, 2H),
3.61 (s, 6H), 3.52 (s, 3H).
EXAMPLE 18
N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide
[0389] A solution of Example 12 (0.909 g) and salcomine (0.082 g)
in methanol (125 mL) was treated with O.sub.2 gas over 18 hours and
concentrated. The concentrate was purified by flash column
chromatography on silica gel with 2% methanol/dichloromethane to
provide the desired compound.
[0390] MS (DCI/NH.sub.3) m/z 363 (M+H).sup.+ 380
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 9.94
(s, 1H), 8.07 (s, 1H), 7.52 (m, 2H), 6.61 (m, 1H), 6.39 (s, 2H),
3.61 (s, 6H), 3.51 (s, 3H).
EXAMPLE 19
1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide
[0391] A solution of Example 18 (1.01 g, 2.8 mmol) in THF (100 mL)
at 0.degree. C. was treated with sodium bis(trimethylsilyl)amide
(1M in THF, 7 mL, 6.89 mmol), stirred for 20 minutes, treated
dropwise with CH.sub.3I (195 .mu.L, 3.1 mmol), stirred over 18
hours while warming to room temperature, treated with water, and
extracted with ethyl acetate. The extract was dried
(Na.sub.2SO.sub.4), filtered and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 1%
methanol/dichloromethane to provide 684 mg of the desired
compound.
[0392] MS (DCI/NH.sub.3) m/z 377 (M+H).sup.+ 394
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
9.94 (s, 1H), 8.06 (t, 1H), 7.57 (m, 2H), 7.49 (d, J=3.3 Hz, 1H),
6.64 (d, J=3.3 Hz, 1H), 6.39 (s, 1H), 3.81 (s, 3H), 3.62 (s, 6H),
3.51 (s, 3H).
EXAMPLE 20
N,1-dimethyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide
[0393] A solution of example Example 18 (50 mg, 0.14 mmol) in THF
at 0.degree. C. was treated portionwise with NaH (60% dispersion in
mineral oil, 26 mg, 0.70 mmol), stired for 20 minutes, treated
dropwise with CH.sub.3I (52 .mu.L, 0.84 mmol), warmed to room
temperature, stirred for 18 hours, treated with water, and
extracted with ethyl acetate (10 mL). The organic layer was washed
sequentially with water (5 mL) and brine (5 mL), dried
(Na.sub.2SO.sub.4), filtered, and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 1%
methanol:dichloromethane to provide 48 of the desired product.
[0394] MS (DCI/NH.sub.3) m/z 391 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 7.86 (d, J=1.5 Hz, 1H), 7.64 (d,
J=8.4 Hz, 1H), 7.54 (d, J=2.7 Hz, 1H), 7.32 (dd, J=1.5, 8.4 Hz,
1H), 6.65 (d, J=2.7 Hz, 1H), 6.29 (s, 2H), 3.86 (s, 3H), 3.64 (s,
3H), 3.58 (s, 6H), 3.06 (s, 3H).
EXAMPLE 21
3,4,5-trimethoxy-N-(4-methoxyphenyl)benenesulfonamide
EXAMPLE 21A
3,4,5-trimethoxybenzenesulfonyl chloride
[0395] The procedure in J. Het. Chem. 23, 1253 (1986) was followed.
A solution of 3,4,5-trimethoxyaniline (5.0 g) in acetic acid (26
mL) and 12M HCl (47 mL) at -10-5.degree. C. was treated slowly with
a solution of NaNO.sub.2 (2 g) in water (7 mL), stirred at
-5.degree. C. for another 30 minutes, added in portions to a cold
(-5.degree. C.) solution of CuCl.sub.2 and SO.sub.2 in acetic acid
(35 mL) and water (6 mL), stirred at -5-0.degree. C. for 3 hours,
warmed to room temperature overnight, poured over ice, filtered,
and dried to provide the desired product.
EXAMPLE 21B
3,4,5-trimethoxy-N-(4-methoxyphenyl)benenesulfonamide
[0396] A solution of 4-methoxyaniline (139 mg, 1.1 mmol) in
pyridine (2 mL) was treated with Example 21A (300 mg, 1.1 mmol) in
THF (2 mL), stirred at room temperature for 18 hours, concentrated,
redissolved in THF (1 mL), treated with water with stirring, and
filtered to provide 300 mg of the desired product.
[0397] MS (DCI/NH.sub.3) m/z 353 (M+H).sup.+ and 371
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 6.99
(d, J=9 Hz, 1H), 6.80 (d, J=9 Hz, 1H), 6.85 (s, 2H), 3.87 (s, 3H),
3.77 (s, 3H), 3.76 (s, 6H).
EXAMPLE 22
N-(3-hydroxy-4-methoxyphenyl)-3,4,5-trimethoxybenzamide
[0398] Example 21A was processed as described in Example 21B
(substituting 5-amino-2-methoxyphenol for 4-methoxyaniline) to
provide the desired product.
[0399] MS (DCI/NH.sub.3) m/z 369 (M+H).sup.+ and 387
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 6.92
(s, 2H), 6.72 (d, J=8.7 Hz, 1H), 6.69 (d, 1H), 6.57 (dd, J=2.7, 8.7
Hz, 1H), 3.87 (s, 3H), 3.85 (s, 3H), 3.78 (s, 6H).
EXAMPLE 23
N-(1-methyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
[0400] Example 21A (456 mg) was processed as described in Example
21B (substituting 1H-indol-5-amine for 4-methoxyaniline) to provide
480 mg of the desired product.
[0401] MS (DCI/NH.sub.3) m/z 377 (M+H).sup.+ and 394
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.32
(d, J=3 Hz, 1H), 7.19 (d, J=8.4 Hz, 1H), 7.05 (d, J=3 Hz, 1H), 6.90
(dd, J=3.0, 8.4 Hz, 1H), 6.86 (s, 2H), 6.40 (d, J=3 Hz, 1H), 3.85
(s, 3H), 3.76 (s, 3H), 3.68 (s, 6H).
EXAMPLE 24
N-(4-(dimethylamino)phenyl)-3,4,5-trimethoxybenzenesulfonamide
[0402] Example 21A (195 mg) was processed as described in Example
21B (substituting N',N'-dimethyl-1,4-benzenediamine for
4-methoxyaniline) to provide 200 mg of the desired product.
[0403] MS (DCI/NH.sub.3) m/z 367 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 9.55 (s, 1H), 6.93 (s, 2H), 6.90
(d, J=9 Hz, 2H), 6.86 (s, 1H), 6.61 (d, J=9 Hz, 2H), 3.75 (s, 3H),
3.74 (s, 6H), 2.81 (s, 6H).
EXAMPLE 25
N-(4-fluoro-3-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide
[0404] Example 21A (250 mg) was processed as described in Example
21B (substituting 3-fluoro-4-methoxyaniline for 4-methoxyaniline)
to provide 310 mg of the desired product.
[0405] MS (DCI/NH.sub.3) m/z 371 (M+H).sup.+ and 389
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.05 (s, 1H), 7.08 (d, J=9.3 Hz, 1H), 6.98 (s, 2H), 6.86 (d, J=9.3
Hz, 1H), 3.76 (s, 6H), 3.70 (s, 3H), 3.64 (s, 3H).
EXAMPLE 26
3,4,5-trimethoxy-N-(4-(trifluoromethoxy)phenyl)benzenesulfonamide
[0406] Example 21A (125 mg) was processed as described in Example
21B (substituting 4-trifluoromethoxyaniline for 4-methoxyaniline)
to provide 125 mg of the desired product.
[0407] MS (DCI/NH.sub.3) m/z 407 (M+H).sup.+ and 425
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.37 (s, 1H), 7.29 (d, J=9.3 Hz, 2H), 7.21 (d, J=9.3 Hz, 2H), 6.99
(s, 2H), 3.75 (s, 6H), 3.69 (s, 3H).
EXAMPLE 27
3,4,5-trimethoxy-N-(2,3,4,5,6-pentafluorophenyl)benzenesulfonamide
[0408] Example 21A (125 mg) was processed as described in Example
21B (substituting 2,3,4,5,6-pentafluoroaniline for
4-methoxyaniline) to provide 120 mg of the desired product.
[0409] MS (DCI/H.sub.3) m/z 413 (M+H).sup.+ and 431
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
7.02 (s, 2H), 3.81 (s, 3H), 3.79 (s, 6H).
EXAMPLE 28
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide
hydrochloride
Example 28A
tert-butyl 2-methoxy-5-nitrophenylcarbamate
[0410] A solution of 2-methoxy-5-nitroaniline (2.0 g, 12 mmol) in
dichloromethane was treated sequentially with NaHCO.sub.3 and
di(tert-butyl) dicarbonate, stirred at room temperature overnight,
treated with DMAP (10 mgs) and triethylamine (600 .mu.L, 4 mmol),
stirred at room temperature for 2 days, neutralized with 1M HCl,
and extracted with ethyl acetate (500 mL). The organic layer was
dried (Na.sub.2SO.sub.4), filtered, and concentrated. The
concentrate was purified by flash chromatography on silica gel with
5% methanol:dichloromethane to provide 3 g of the desired
product.
EXAMPLE 28B
tert-butyl 5-amino-2-methoxyphenylcarbamate
[0411] A solution of Example 28A in 1:1 THF:methanol (40 mL) was
treated with 10% palladium on carbon (800 mg), stirred under
hydrogen (1 atm) for one hour, filtered through diatomaceous earth
(Celite.RTM.), and concentrated to provide the desired product.
EXAMPLE 28C
N-(3-tert-butoxycarbonylamino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulf-
onamide
[0412] Example 21A (200 mg) was processed as described in Example
21B (substituting Example 28B for 4-methoxyaniline) to provide 300
mg of the desired product.
EXAMPLE 28D
N-(3-amino-4-methoxyphenyl)-3,4,5-trimethoxybenzenesulfonamide
hydrochloride
[0413] A solution of Example 28C in dichloromethane (1 mL) was
treated with 4.0M HCl in dioxane, stirred for 1 hour, concentrated
to a fraction of its original volume, and treated with diethyl
ether to precipitate 250 mg of the desired product.
[0414] MS (DCI/NH.sub.3) m/z 369 (M+H).sup.+ and 386
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
10.01 (s, 1H), 7.03 (d, 1H), 7.00 (s, 2H), 6.97 (d, 1H), 6.94 (s,
1H), 3.77 (s, 6H), 3.70 (s, 3H).
EXAMPLE 29
3,4,5-trimethoxy-N-(1-methyl-1H-indol-4-yl)benzenesulfonamide
EXAMPLE 29A
1-methyl-4-nitro-1H-indole
[0415] A solution of 4-nitroindole (500 mg, 3.1 mmol) in THF (15
mL) at 0.degree. C. was treated portionwise with NaH (290 mg, 9.3
mmol), stirred for 30 minutes, treated dropwise with methyl iodide
(0.95 mL, 15.5 mmol), warmed to room temperature for 18 hours,
treated sequentially with water and ethyl acetate (200 mL), and
washed with brine (100 mL). The organic layer was dried
(Na.sub.2SO.sub.4), filtered, and concentrated to provide the
desired product, which was used in the next step without further
purification.
EXAMPLE 29B
1-methyl-1H-indol-4-amine
[0416] Example 29A (500 mg) was processed as described in Example
28B to provide 400 mg of the desired product.
EXAMPLE 29C
3,4,5-trimethoxy-N-(1-methyl-1H-indol4-yl)benzenesulfonamide
[0417] Example 29B (82 mg, 0.56 mmol) was dissolved in pyridine (1
mL), treated portionwise with Example 21A, (150 mg, 0.56 mmol) in
THF (1 mL), stirred for 18 hours, concentrated, treated with a
small amount of THF to dissolve the concentrate, and treated with
water with vigorous stirring. The precipitate was filtered and
dried in a vacuum oven to provide 180 mg of the desired
product.
[0418] MS (DCI/NH.sub.3) m/z 377 (M+H).sup.+ and 394
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 9.93
(s, 1H), 7.20 (d, J=3 Hz, 1H), 7.18 (d, J=6 Hz, 1H), 7.05 (d, J=7.5
Hz, 1H), 6.99 (s, 2H), 6.95 (dd, J=0.6, 7.5 Hz, 1H), 3.71 (s, 3H),
3.68 (s, 6H), 3.64 (s, 3H).
EXAMPLE 30
3,4,5-trimethoxy-N-(1-methyl-1H-indol-6-yl)benzenesulfonamide
[0419] Example 21A (150 mg) was processed as described in Example
21B (substituting 1-methyl-1H-indol-6-amine for 4-methoxyaniline)
to provide 200 mg of the desired product.
[0420] MS (DCI/NH.sub.3) m/z 377 (M+H).sup.+ and 394
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
9.92 (s, 1H), 7.39 (d, J=8.4 Hz, 1H), 7.25 (d, J=3 Hz, 1H), 7.16
(s, 1H), 7.00 (s, 2H), 6.78 (dd, J=1.85, 8.4 Hz, 1H), 6.33 (d, 1H),
3.70 (s, 6H), 3.68 (s, 3H), 3.66 (s, 3H).
EXAMPLE 31
N-(1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
[0421] Example 21A (100 mg) was processed as described in Example
21B (substituting 1H-indol-5-amine for 4-methoxyaniline) to provide
110 mg of the desired product.
[0422] MS (DCI/NH.sub.3) m/z 363 (M+H).sup.+ and 380
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
11.06 (s, 1H), 9.72 (s, 1H), 7.31 (t, J=2.7 Hz, 1H), 7.26 (d, J=1.8
Hz, 1H), 7.24 (s, 1H), 6.95 (s, 2H), 6.85 (dd, J=1.8, 8.7 Hz, 1H),
6.35 (t, J=2.7 Hz, 1H), 3.69 (s, 6H), 3.66 (s, 3H).
EXAMPLE 32
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
Example 32A
1,2-dimethyl-1H-indol-5-amine
[0423] A solution of 1,2-dimethyl-5-nitro-1H-indole (500 mg) in 1:1
THF:methanol (10 mL) was treated with 10% palladium on carbon (100
mg), stirred under hydrogen (1 atm) for 1 hour, filtered through
diatomaceous earth (Celite.RTM.), and concentrated. The crude
product was used without further purification in the next step.
EXAMPLE 32B
N-(1,2-dimethyl-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
[0424] Example 21A (100 mg) was processed as described in Example
23B (substituting Example 32A for 4-methoxyaniline) to provide 115
mg of the desired product.
[0425] MS (DCI/NH.sub.3) m/z 391 (M+H).sup.+ and 408
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
9.71 (s, 1H), 7.23 (d, J=9 Hz, 1H), 7.15 (d, J=1.8 Hz, 1H), 6.96
(s, 2H), 6.82 (dd, J=1.8, 9 Hz, 1H), 6.12 (s, 1H), 3.70 (s, 6H),
3.66 (s, 3H), 3.58 (s, 3H), 2.34 (s, 3H).
EXAMPLE 33
N-(3-chloro-1H-indol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
[0426] A solution of Example 31 (51 mg, 0.14 mmol) in
dichloromethane (1.5 mL) and DMF (50 .mu.L) was treated with
N-chlorosuccinimide (21 mg, 0.15 mmol), stirred for 2 hours, and
treated sequentially with water (1 mL) and ethyl acetate (10 mL).
The organic layer was washed sequentially with 0.2 MHCl (5 mL) and
saturated aqueous NaHCO.sub.3 (5 mL), dried (Na.sub.2SO.sub.4),
filtered, and concentrated to provide 25 mg of the desired
product.
[0427] MS (DCI/NH.sub.3) 414 (M+NH.sub.4).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 9.88 (s, 1H), 7.49 (d, 1H), 7.32
(s, 1H), 7.29 (s, 1H), 7.18 (d, J=1.8 Hz, 1H), 6.97 (s, 2H), 6.94
(d, J=1.8 Hz, 1H), 3.70 (s, 6H), 3.67 (s, 3H).
EXAMPLE 34
N-(1H-indazol-5-yl)-3,4,5-trimethoxybenzenesulfonamide
[0428] Example 21A (100 mg) was processed as described in Example
21B (substituting 1H-indazol-5-amine for 4-methoxyaniline) to
provide 110 mg of the desired product.
[0429] MS (DCI/NH.sub.3) m/z 364 (M+H).sup.+ and 381
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
13.02 (s, 1H), 9.95 (s, 1H), 8.00 (s, 1H), 7.45 (d, J=2.1 Hz, 1H),
7.42 (s, 1H), 7.11 (dd, J=2.1, 8.7 Hz, 1H), 6.95 (s, 2H), 3.69 (s,
6H), 3.66 (s, 3H).
EXAMPLE 35
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-6-yl)
benzenesulfonamide
[0430] 5-Nitrobenzimidazole (500 mg) was processed as described for
4-nitroindole in Examples 29A, 29B, and 29C to provide both
methylated isomers. The isomers were separated by flash column
chromatography on silica gel with 1% methanol:dichloromethane to
provide 133 mg of the desired compound as the less polar
isomer.
[0431] MS (DCI/NH.sub.3) m/z 378 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 10.08 (s, 1H), 8.10 (s, 1H), 7.50
(d, J=8.4 Hz, 1H), 7.30 (d, J=1.8 Hz, 1H), 7.01 (s, 2H), 6.93 (dd,
J=1.8, 8.4 Hz, 1H), 3.75 (s, 3H), 3.71 (s, 6H), 3.67 (s, 3H).
EXAMPLE 36
3,4,5-trimethoxy-N-(1-methyl-1H-benzimidazol-5-yl)benzenesulfonamide
[0432] 5-Nitrobenzimidazole (500 mg) was processed as described in
Example 35 to provide 167 mg of the desired compound as the more
polar isomer.
[0433] MS (DCI/NH.sub.3) m/z 378 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6, 300 MHz) .delta. 9.98 (s, 1H), 8.13 (s, 1H), 7.44
(d, J=8.7 Hz, 1H), 7.35 (d, J=1.8 Hz, 1H), 7.05 (dd, J=1.8, 8.7 Hz,
1H), 6.99 (s, 2H), 3.77 (s, 3H), 3.71 (s, 6H), 3.66 (s, 3H).
EXAMPLE 37
3,4,5-trimethoxy-N-methyl-N-(1-methyl-1H-indol-5-yl)
benzenesulfonamide
[0434] A solution of Example 23 (50 mg, 0.13 mmol) in THF (1 mL) at
0.degree. C. was treated with NaH (60% suspension in mineral oil,
15 mg, 0.39 mmol) in portions, stirred at 0.degree. C. for 30
minutes, treated dropwise with methyl iodide (42 .mu.L, 0.65 mmol
warmed to room temperature overnight. The product was absorbed on
silica gel and purified by flash column chromatography using 1%
Methanol:Dichloromethane as the eluent. This afforded the product
as a solid (35 mg, 68%) plus some unreacted starting material.
[0435] MS (DCI/NH.sub.3) m/z 391 (M+H).sup.+ and 408
(M+NH.sub.4).sup.+; .sup.1H NMR (DMSO-d.sub.6, 300 MHz) .delta.
7.40 (d, J=8.7 Hz, 1H), 7.37 (d, J=3 Hz, 1H), 7.25 (d, J=2.1 Hz,
1H), 6.87 (dd, J=2.1, 8.7 Hz, 1H), 6.68 (s, 2H), 6.27 (d, J=8.1 Hz,
1H), 4.03 (s, 3H), 3.75 (s, 3H), 3.73 (s, 6H).
EXAMPLE 38
3,4,5-trimethoxy-N-(2-(dimethylamino)ethyl)-N-(1-methyl-1H-indol-5-yl)benz-
enesulfonamide
[0436] A solution of Example 23 (50 mg, 0.13 mmol) in THF (2 mL)
was treated sequentially with triphenylphosphine (52 mh, 0.19
mmol), N,N-dimethyl-2-aminoethanol (17 .mu.L, 0.16 mmol), and
diethylazodicarboxylate (31 .mu.L, 0.19 mmol), stirred at room
temperature overnight, treated with silica gel, and concentrated.
The mixture was purified by flash column chromatography on silica
gel with 1% methanol:dichloromethane to provide 46 mg of the
desired product.
[0437] MS (DCI/NH.sub.3) m/z 448 (M+H).sup.+ and 470 (M+Na).sup.+;
.sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.40 (d, J=8.7 Hz, 1H),
7.38 (d, J=3 Hz, 1H), 7.26 (d, J=2.1 Hz, 1H), 6.83 (dd, J=2.1, 8.7
Hz, 1H), 6.79 (s, 2H), 6.41 (d, J=3 Hz, 1H), 3.78 (s, 3H), 3.75 (s,
3H), 3.70 (s, 6H), 3.64 (t, 2H), 2.22 (t, 2H) 2.08 (s, 6H).
EXAMPLE 39
1H-indol-5-ol, (3,4,5-trimethoxybenzenesulfonate) ester
[0438] A solution of 5-hydroxyindole (CAS number 13523-92-7, 253
mg, 1.9 mmol) in dichloromethane (15 mL) and pyridine (0.5 mL) was
treated sequentially with Example 21A (507 mg, 1.9 mmol) and a
catalytic amount of DMAP, stirred for 1 week, and washed with
saturated CuSO.sub.4. The organic layer was dried (MgSO.sub.4),
filtered, and concentrated. Chromatography of the concentrate on
silica gel with 30% ethyl acetate/hexane provided 520 mg of the
desired compound as a white crystalline solid.
[0439] MS (ESI/NH.sub.3) m/z 364 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 8.24 (br s, 1H), 7.29-7.25 (m, 3H), 7.02 (s,
2H), 6.82 (dd, J=2.2, 8.8, 1H), 6.51 (m, 1H), 3.92 (s, 3H), 3.78
(s, 6H).
EXAMPLE 40
3,4,5-trimethoxyphenyl) 4-methoxybenzenesulfonate
[0440] 3,4,5-trimethoxyphenol (504 mg) was processed as described
in Example 43 (substituting 4-methoxybenzenesulfonyl chloride for
4-methoxymetanilyl fluoride) to provide 500 mg of the desired
product.
[0441] MS (ESI/NH.sub.3) m/z 355 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 7.81-7.77 (m, 2H), 7.01-6.98 (m, 2H), 6.20 (s,
2H), 3.89 (s, 3H), 3.80 (s, 3H), 3.72 (s, 6H).
EXAMPLE 41
(3 4,5-trimethoxyphenyl) 4-methylbenzenesulfonate
[0442] 3,4,5-trimethoxyphenol (497 mg) was processed as described
in Example 43 (substituting para-toluenesulfonyl chloride for
4-methoxymetanilyl fluoride) to provide 770 mg of the desired
product.
[0443] MS (ESI/NH.sub.3) m/z 339 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 7.75 (d, J=8.5, 2H), 7.34 (d, J=8.1, 2H), 6.19
(s, 2H), 3.80 (s, 3H), 3.70 (s, 6H), 2.46 (s, 3H).
EXAMPLE 42
1H-indol-5-yl 3,4,5-trimethoxybenzenesulfonate
[0444] Example 21A (726 mg) was processed as described in Example
39 (substituting 1-methyl-1H-indol-5-ol for 5-hydroxyindole to
provide 650 mg of the desired product. MS (ESI/NH.sub.3) m/z 395
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.24
(d, J=2.4, 1H), 7.19 (d, J=8.8, 1H), 7.09 (d, J=3.1, 1H), 7.02 (s,
2H), 6.85 (dd, J=2.4, 8.8, 1H), 6.43 (dd, J=0.7, 3.1, 1H), 3.92 (s,
3H), 3.79 (s, 6H), 3.77 (s, 3H).
EXAMPLE 43
(3,4,5-trimethoxyphenyl) 3-amino-4-methoxybenzenesulfonate
[0445] A solution of 3,4,5-trimethoxyphenol (505.7 mg, 2.75 mmol)
in dichloromethane (28 mL) was treated sequentially with
triethylamine (1.2 mL), 4-methoxymetanilyl fluoride (572.5 mg, 2.75
mmol), and tert-butylammonium iodide (106 mg, 0.287 mmol), stirred
overnight, and washed once with 1M Na.sub.2CO.sub.3. The organic
layer was separated, dried (MgSO.sub.4), filtered, and
concentrated. Chromatography of the concentrate on silica gel with
40% ethyl acetate/hexanes provided 356 mg of the desired
compound.
[0446] MS (ESI/NH.sub.3) m/z 370 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 7.25-7.20 (m, 2H). 6.84 (d, J=8.5, 1H), 6.23
(s, 2H), 3.93 (s, 3H), 3.75 (s, 3H), 3.73 (s, 6H).
EXAMPLE 44
(3,4,5-trimethoxyphenyl)-4-(dimethylamino)benzenesulfonate
[0447] 4-(Dimethylamino)benzenesulfonyl chloride (prepared as
described in J. Am. Chem. Soc. 1997, 99:3, 851-858, 1.93 g) was
processed as described for 4-methoxymetanilyl fluoride in Example
43 to provide 2.55 g of the desired product.
[0448] MS (ESI/NH.sub.3) m/z 368 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 7.66 (d, J=8.8, 2H), 6.66 (d, J=9.2, 2H), 6.22
(s, 2H), 3.79 (s, 3H), 3.71 (s, 6H), 3.07 (s, 6H).
EXAMPLE 45
4-methylphenyl 3,4,5-trimethoxybenzenesulfonate
[0449] Example 21A (103 mg) was processed as described in Example
39 (substituting 4-methylphenol for 5-hydroxyindole to provide 26.6
mg of the desired product.
[0450] MS (ESI/NH.sub.3) m/z 356 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, CDCl.sub.3) .delta. 7.11-7.08 (m, 2H), 7.01 (s, 2H),
6.90-6.87 (m, 2H), 3.92 (s, 3H), 3.83 (s, 6H), 2.32 (s, 3H).
EXAMPLE 46
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate
EXAMPLE 46A
1-methyl-5-indolinesulfonyl chloride
[0451] A solution of chlorosulfonic acid at 0.degree. C. (5 mL, 75
mmol) was treated portionwise with 1-methylindoline (CAS No.
[824-21-5], 1.96 g, 14.7 mmol), warmed to room temperature, heated
at 75.degree. C. for 40 minutes, cooled, and poured onto ice to
provide a solid. The solid was collected by suction filtration,
washed with water, and dried to provide 927 mg of the desired
product.
[0452] .sup.1H NMR (300 MHz, CDCl.sub.3) 7.33 (dd, J=1.8, 7.7, 1H),
7.19 (d, J=7.7, 1H), 6.93 (d, J=1.8, 1H), 3.51 (t, J=8.5, 2H), 3.07
(t, J=8.5, 2H), 2.86 (s, 3H).
EXAMPLE 46B
3,4,5-trimethoxyphenyl 1-methyl-5-indolinesulfonate
[0453] 3,4,5-trimethoxyphenol (103 mg) was processed as described
in Example 43 (substituting Example 52A for 4-methoxymetanilyl
fluoride) to provide 22 mg of the desired product.
[0454] MS (ESI/NH.sub.3) m/z 380 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) .delta. 7.12 (s, 2H), 6.79 (s, 1H), 6.24 (s, 2H), 3.80
(s, 3H), 3.72 (s, 6H), 3.46 (t, 2H), 3.02 (t, 2H), 2.77 (s,
3H).
EXAMPLE 47
4-methoxyphenyl 3,4,5-trimethoxybenzenesulfonate
[0455] Example 21A (104 mg) was processed as described in Example
39 (substituting 4-methoxyphenol for 5-hydroxyindole to provide
69.6 mg of the desired product. MS (ESI/NH.sub.3) m/z 372
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.00
(s, 2H), 6.93-6.89 (m, 2H), 6.82-6.78 (m, 2H), 3.92 (s, 3H), 3.83
(s, 6H), 3.68 (s, 3H).
EXAMPLE 48
tert-butyl
2-((1-methyl-1H-indol-5-yl)((3,4,5-trimethoxyphenyl)sulfonyl)am-
ino)ethylcarbamate
[0456] Example 23 (50 mg) was processed as described in Example 38
(substituting N-(tert-butoxycarbonyl)ethanolamine for
N,N-dimethyl-2-aminoethanol) to provide 60 mg of the desired
product.
[0457] MS (DCI/NH.sub.3) m/z 520 (M+H).sup.+ and 537
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
7.40 (d, 1H), 7.38 (d, J=3 Hz, 1H), 7.28 (d, 1H), 6.83 (m, 1H),
6.76 (s, 2H), 6.40 (d, J=3 Hz, 1H), 3.79 (s, 3H), 3.75 (s, 3H),
3.71 (s, 6H), 3.57 (s, 2H), 3.16 (d, 1H), 2.96 (q, 2H), 1.32 (s,
9H).
EXAMPLE 49
N-(2-hydroxyethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfo-
namide
[0458] Example 23 (50 mg) was processed as described in Example 38
(substituting ethylene glycol for N,N-dimethyl-2-aminoethanol) to
provide 60 mg of the desired product.
[0459] MS (DCI/NH.sub.3) m/z 420 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 8.97 (s, 1H), 7.28 (d, J=2.7 Hz, 1H), 7.26
(d, J=2.1 Hz, 1H), 6.96 (s, 2H), 6.90 (dd, J=8.4 Hz, 2.1 Hz, 1H),
6.34 (d, J=2.7 Hz, 1H), 4.03 (q, J=6.9 Hz, 2H), 3.72 (s, 3H), 3.69
(s, 3H), 3.66 (s, 6H), 1.17 (t, J=6.9 Hz, 2H).
EXAMPLE 50
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3,4,5-trimethoxybenzenesulfonamide
[0460] Example 21A (500 mg) was processed as described in Example
21B (substituting 1,4-benzodioxane-6-amine for 4-methoxyaniline) to
provide 651 mg of the desired product.
[0461] MS (DCI/NH.sub.3) m/z 381 (M+H).sup.+ and 399
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
9.85 (s, 1H), 6.97 (s, 2H), 6.73 (d, 1H), 6.57 (m, 2H), 4.16 (s,
2H), 3.76 (s, 6H), 3.70 (s, 3H).
EXAMPLE 51
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfona-
mide hydrochloride
EXAMPLE 51A
benzyl
2-((1-methyl-1H-indol-5-yl)((3,4,5-trimethoxyphenyl)sulfonyl)amino)-
ethylcarbamate
[0462] Example 23 (50 mg) was processed as described in Example 38
(substituting benzyl N-(2-hydroxyethyl) carbamate for
N,N-dimethyl-2-aminoethanol) to provide 46 mg of the desired
product.
EXAMPLE 51B
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfona-
mide
[0463] A solution of Example 51A in methanol:THF (1 mL:1 mL) was
treated with 10% palladium on carbon (30 mg), stirred under with
hydrogen (1 atm) for 2 hours, filtered through diatomaceous earth
(Celite.RTM.), and concentrated to provide the desired
compound.
EXAMPLE 51C
N-(2-aminoethyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfona-
mide hydrochloride
[0464] Example 51B, in less than 1 mL of methanol, was treated with
several drops of 1M HCl in diethyl ether to provide a cloudy,
acidic solution. Diethyl ether was added to cause precipitation.
The precipitate was filtered and oven dried to provide 20 mg of the
desired product.
[0465] MS (DCI/NH.sub.3) m/z 420 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.41 (d, J=8.7 Hz, 1H), 7.38 (d, J=3 Hz, 1H),
7.28 (d, J=2.1 Hz, 1H), 6.83 (dd, J=8.7 Hz, 2.1 Hz, 1H), 6.77 (s,
2H), 6.41 (d, J=3 Hz, 1H), 3.79 (s, 3H), 3.76 (s, 3H), 3.70 (s,
6H).
EXAMPLE 52
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
Example 52A
4-methoxy-N-methyl-3-nitro-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0466] A solution of Example 9B (100 mg, 0.25 mmol) in THF (2 mL)
at .degree. C. was treated with sodium hydride (29 mg, 60%
dispersion in mineral oil, 0.75 mmol), stirred for 20 minutes,
treated dropwise with methyl iodide (94 .mu.L, 1.5 mmol), warmed to
room temperature, stirred for 18 hours, treated with water, and
extracted with ethyl acetate. The extract was concentrated, and the
concentrate was used in the next step without further
purification.
EXAMPLE 52B
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0467] A solution of Example 52A (00 mg) in methanol:THF (1 mL:1
mL) was treated with 10% palladium on carbon, stirred under
hydrogen (1atm) for 4 hours, filtered through diatomaceous earth
(Celite.RTM.), and concentrated. The concentrate was purified by
flash column chromatography on silica gel with 2%
methanol/dichloromethane to provide 31 mg of the desired
product.
EXAMPLE 52C
3-amino-4-methoxy-N-methyl-N-(3,4,5-trimethoxyphenyl)benzenesulfonamide
[0468] A solution of Example 52B in less than 1 mL of methanol was
treated with several drops of 1M HCl in diethyl ether to provide a
cloudy, acidic solution and treated with diethyl ether to
precipitate the product. The precipitate was filtered and oven
dried to provide 13.5 mg of the desired product.
[0469] MS (DCI/NH.sub.3) m/z 383 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 6.83 (d, J=2.1 Hz, 1H), 6.75 (d, J=8.1 Hz,
1H), 6.66 (dd, J=8.1 Hz, 2.1 Hz, 1H), 6.31 (s, 2H), 3.66 (s, 6H),
3.64 (s, 3H), 3.02 (s, 3H).
EXAMPLE 53
1-ethyl-N-(3 4 5-trimethoxyphenyl)-1H-indole-5-sulfonamide
[0470] Example 18 (50 mg) was processed as described in Example 19
(substituting ethyl iodide for methyl iodide) to provide 40 mg of
the desired product.
[0471] MS (DCI/NH.sub.3) m/z 391 (M+H).sup.+ and 408
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
9.93 (s, 1H), 8.06 (d, J=2.1 Hz, 1H), 7.70 (d, J=3 Hz, 1H), 7.63
(m, 1H), 7.60 (d, J=2.1 Hz, 1H), 6.62 (d, J=3 Hz, 1H), 6.39 (s,
2H), 4.23 (q, J=7.5 Hz, 2H), 3.61 (s, 6H), 3.52 (s, 3H), 1.34 (t,
J=7.5 Hz, 3H).
EXAMPLE 54
N-acetyl-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfonamide
[0472] A solution of Example 23 (50 mg, 0.13 mmol) in THF (1 mL) at
-25.degree. C. was treated with sequentially with triethylamine (24
.mu.L, 0.17 mmol), lithium chloride (6 mg, 0.14 mmol), and acetic
anhydride (25 .mu.L, 0.26 mmol)dropwise, warmed to room
temperature, stirred for 18 hours, and concentrated. The
concentrate was treated with ethyl acetate and washed with water
and brine. The organic layer was dried (Na.sub.2SO.sub.4), filtered
and concentrated. The concentrate was purified by flash column
chromatography on silica gel with 1% methanol:dichloromethane to
provide 35 mg of the desired product.
[0473] MS (DCI/NH.sub.3) m/z 419 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.59 (d, J=2.1 Hz, 1H), 7.57 (d, J=8.7 Hz,
1H), 7.47 (d, J=3.3 Hz, 1H), 7.17 (s, 2H), 7.13 (dd, J=8.7, 2.1 Hz,
1H), 6.53 (dd, J=2.1, 3.3 Hz, 1H), 3.86 (s, 6H), 3.85 (s, 3H), 3.73
(s, 3H), 1.81 (s, 3H).
EXAMPLE 55
3,4,5-trimethoxy-N-(6-quinolinyl)benzenesulfonamide
[0474] Example 21A (185 mg) was processed as described in Example
21B (substituting 6-aminoquinoline for 4-methoxyaniline) to provide
180 mg of the desired product.
[0475] MS (DCI/NH.sub.3) m/z 375 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 10.58 (s, 1H), 8.77 (dd, J=1.5 Hz, 4.0 Hz,
1H), 8.31 (d, J=8.7 Hz 1H), 7.91 (d, J=9 Hz, 1H), 7.68 (d, J=2.4,
1H), 7.53 (dd, J=9 Hz, 2.4 Hz, 1H), 7.46 (dd, J=8.7 Hz, 4.2 Hz,
1H), 3.73 (s, 6H), 3.65 (s, 3H).
EXAMPLE 56
N-(2-hydroxyethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfona-
mide
[0476] Example 19 (50 mg) was processed as described in Example 38
(substituting ethylene glycol for N,N-dimethyl-2-aminoethanol) to
provide 25.5 mg of the desired product.
[0477] MS (DCI/NH.sub.3) m/z 421 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.86 (d, J=1.8 Hz, 1H), 7.64 (d, J=8.7 Hz,
1H), 7.54 (d, J=3.3 Hz, 1H), 7.32 (dd, J=8.7 Hz, 1.8 Hz, 1H), 6.65
(d, J=3.3 Hz, 1H), 6.29 (s, 2H), 4.03 (q, J=6.9 Hz, 2H), 3.84 (s,
3H), 3.64 (s, 3H), 3.58 (s, 6H), 2.52 (d, J=6.9 Hz, 2H).
EXAMPLE 57
N-(2-fluoroethyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonam-
ide
[0478] Example 19 (50 mg) was processed as described in Example 38
(substituting 2-fluoroethanol for N,N-dimethyl-2-aminoethanol) to
provide 30 mg of the desired product.
[0479] MS (DCI/NH.sub.3) m/z 423 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.90 (d, J=1.8 Hz, 1H), 7.64 (d, J=8.7 Hz,
1H), 7.54 (d, J=3.3 Hz, 1H), 7.40 (dd, J=8.7 Hz, 1.8 Hz, 1H), 6.63
(d, J=3.3 Hz, 1H), 6.23 (s, 2H), 4.48 (t, 2H), 4.32 (t, 2H), 3.86
(s, 3H), 3.65 (s, 3H), 3.54 (s, 6H).
EXAMPLE 58
N-ethyl-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonamide
[0480] Example 19 (50 mg) was processed as described in Example 38
(substituting ethanol for N,N-dimethyl-2-aminoethanol) to provide
35 mg of the desired product.
[0481] MS (DCI/NH.sub.3) m/z 405 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.89 (d, J=1.8 Hz, 1H), 7.64 (d, J=8.7 Hz,
1H), 7.54 (d, J=3.3 Hz, 1H), 7.40 (dd, J=8.7 Hz, 1.8 Hz, 1H), 6.64
(d, J=3.3 Hz, 1H), 6.22 (s, 2H), 3.86 (s, 3H), 3.65 (s, 3H), 3.56
(s, 6H), 3.49 (q, 2H), 0.97 (t, 3H).
EXAMPLE 59
4-nitrophenyl-3,4,5-trimethoxybenzenesulfonate
[0482] A solution of 21A (2.02 g, 7.57 mmol) in dichloromethane (31
mL) was treated with 4-nitrophenol (1.06 g, 7.62 mmol), pyridine,
and DMAP, stirred overnight at room temperature, diluted with ethyl
acetate and washed with 1M Na.sub.2CO.sub.3. The organic layer was
dried (MgSO.sub.4), filtered, and concentrated to provide 1.76 g of
the title compound.
[0483] MS (APCI) m/z 368 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) 8.23 (m, 2H), 7.23 (m, 2H), 7.05 (s, 2H), 3.94 (s, 3H),
3.86 (s, 6H).
EXAMPLE 60
4-aminophenyl-3,4,5-trimethoxybenzenesulfonate
[0484] A slurry of Example 59 (917 mg, 2.48 mmol) and Pd/C in ethyl
acetate was stirred under hydrogen for 18 hours, filtered and
concentrated to provide 741 mg of the desired product.
[0485] MS (ESI) m/z 357 (M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) 7.01 (s, 2H), 6.80 (d, J=8.1, 2H), 6.61 (d, J=8.5, 2H),
3.93 (s, 3H), 3.85 (s, 6H).
EXAMPLE 61
4-dimethylaminophenyl-3,4,5-trimethoxybenzenesulfonate
[0486] A stirred solution of Example 60 (142 mg, 0.418 mmol) in
acetic acid (4.5 mL) was treated with paraformaldehyde (126 mg, 419
mmol) and NaCNBH.sub.3 (131 mg, 2.09 mmol), stirred overnight at
room temperature, and concentrated. The concentrate was dissolved
in ethyl acetate, washed with saturated sodium bicarbonate, dried
(MgSO.sub.4), filtered, and concentrated to provide 76 mg of the
desired product.
[0487] MS (ESI) m/z 368 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) 7.01 (s, 2H), 6.84 (d, J=8.8, 2H), 6.57 (d, J=8.8, 2H),
3.92 (s, 3H), 3.83 (s, 6H).
EXAMPLE 62
3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate
EXAMPLE 62A
2-methoxy-5-pyridinesulfonylchloride
[0488] The procedure in J. Het. Chem. 23, 1253 (1986) was slightly
modified to make the title compound. A stirred solution of
2-methoxy-5-aminopyridine (1.06 g, 8.61 mmol) in acetic acid (8.2
mL) and 12M HCl (15 mL) at -10.degree. C. was slowly treated with a
solution of NaNO.sub.2 (633 mg, 9.17 mmol) in water (2.2 mL),
stirred at -5.degree. C. for 30 minutes, treated sequentially with
a solution of CuCl.sub.2 (463 mg, 3.44 mmol), SO.sub.2 (4 mL) in
acetic acid (11 mL), and water (2 mL), stirred for 2 hours at
-5.degree. C. and at room temperature for 18 hours, poured over
ice, filtered, and concentrated to provide 725 mg of the desired
product. .sup.1H NMR (300 MHz, CDCl.sub.3) 8.84 (d, J=2.7, 1H),
8.11 (dd, J=2.7, 8.8, 1H), 6.91 (d, J=9.2, 1H), 4.07 (s, 3H).
EXAMPLE 62B
3,4,5-trimethoxyphenyl 6-methoxy-3-pyridinesulfonate
[0489] A solution of 2-methoxypyridine-5-sulfonylchloride (314 mg,
1.51 mmol) in dichloromethane (15 mL) was treated with
triethylamine (0.63 mL) and 3,4,5-trimethoxyphenol (279 mg, 1.51
mmol), stirred for 18 hours at room temperature, diluted with ethyl
acetate, washed three times with 1M Na.sub.2CO.sub.3, dried
(MgSO.sub.4), filtered, and concentrated to provide 447 mg of the
desired product.
[0490] MS (ESI) m/z 356 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) 8.61 (d, J=2.7, 1H), 7.96 (dd, J=2.4, 11.2, 1H), 6.85
(dd, J=0.7, 9.5, 1H), 6.26 (s, 2H), 4.02 (s, 3H), 3.80 (s, 3H),
3.75 (s, 6H).
EXAMPLE 63
1-methyl-2-oxo-1,2-dihydro-4-pyridinyl
3,4,5-trimethoxybenzenesulfonate
[0491] A solution of 4-hydroxy-1-methyl-1H-pyridin-2-one
([40357-87-7], 44.9 mg, 0.358 mmol) in dioxane (2 mL) and DMF (1
mL) was treated with triethylamine (0.15 mL) and
3,4,5-trimethoxybenzenesulfonylchloride (97.1 mg, 0.358 mmol),
stirred for 48 hours at room temperature, diluted with ethyl
acetate, washed with 1.1M NaHSO.sub.4, dried (MgSO.sub.4), filtered
and concentrated. Chromatography of the concentrate on silica gel
with 40% ethyl acetate/hexane provided 47 mg of the desired
compound.
[0492] MS (ESD m/z 356 (M+H).sup.+; .sup.1H NMR (300 MHz,
CDCl.sub.3) 7.29 (d, J=7.5, 1H), 7.11 (s, 2H), 6.21 (d, J=2.4, 1H),
6.13 (dd, J=2.7, 7.5, 1H), 3.94 (s, 3H), 3.92 (s, 6H), 3.51 (s,
3H).
EXAMPLE 64
3,4,5-trimethoxyphenyl
3-((3-aminopropanoyl)amino)-4-methoxybenzenesulfona- te
[0493] A solution of Example 43 (0.148 g, 0.400 mmol) and
N-tert-Boc-.beta.-alanine (0.228 g, 1.20 mmol) in DMF (4 mL) was
treated with HOOBT (0.260 g, 1.60 mmol),
1-ethyl-3-(3-dimethylaminopropyl)carbodi- imide (EDCI) (0.306 g,
1.60 mmol), and triethylamine (0.162 g, 1.60 mmol), heated at
50.degree. C. for 30 hours, cooled, diluted with ethyl acetate (40
mL), washed sequentially with 2:1:1 water/saturated aqueous sodium
bicarbonate/brine (20 mL followed by 2.times.10 mL) and brine (10
mL), filtered through silica gel with ethyl acetate rinses, and
concentrated. The concentrate was purified by radial chromatography
with 1:1 hexane/ethyl acetate to provide 0.138 g of the N-Boc
amide. The amide was stirred in 4M HCl in dioxane (2 mL) for two
hours and concentrated. The concentrate was dissolved in a minimal
amount of water and lyophilized to provide 0.123 g of the desired
product.
[0494] LRMS (ESI(+)) m/z 441 (M+H).sup.+; (ESI(-)) m/z 439
(M-H).sup.-; .sup.1H NMR (DMSO-d.sub.6) .delta. 2.82 (t, J=6.4 Hz,
3H), 3.06 (t, J=6.4 Hz, 3H), 3.61 (s, 3H), 3.63 (s, 6H), 3.96 (s,
3H), 6.30 (s, 2H), 7.29 (d, J=8.8 Hz, 1H), 7.60 (dd, J=2.2, 8.8 Hz,
1H), 7.75-7.81 (br, 3H), 8.62 (d, J=2.2 Hz, 1H), 9.85-9.89 (br,
1H).
EXAMPLE 65
3,4,5-trimethoxyphenyl
3-(((2R)-2-aminopropanoyl)amino)-4-methoxybenzenesu- lfonate
[0495] Example 43 was processed as described in Example 64 but
substituting N-t-Boc-alanine for N-tert-Boc-.beta.-alanine to
provide the desired product.
[0496] LRMS (ESI(+)) m/z 441 (M+H).sup.+; (ESI(-)) n/z 439
(M-H).sup.-; .sup.1H NMR (DMSO-d.sub.6) .delta. 1.44 (d, J=6.7 Hz,
3H), 3.61 (s, 3H), 3.63 (s, 6H), 3.98 (s, 3H), 4.19-4.28 (br, 1H),
6.31 (s, 2H), 7.43 (d, J=8.9 Hz, 1H), 7.67 (dd, J=2.4, 8.9 Hz, 1H),
8.25-8.32 (br, 3H), 8.49 (d, J=2.4 Hz, 1H), 10.17 (s, 1H).
EXAMPLE 66
3,4,5-trimethoxyphenyl
3-(((2R)-2-amino-3-methylbutanoyl)amino)-4-methoxyb-
enzenesulfonate
[0497] Example 43 was processed as described in Example 64 but
substituting N-t-Boc-valine for N-tert-Boc-.beta.-alanine to
provide the desired product.
[0498] LRMS (ESI(+)) m/z 469 (M+H).sup.+; (ESI(-)) m/z 467
(M-H).sup.-; .sup.1H NMR (DMSO-d.sub.6) .delta. 0.98 (d, J=7.0 Hz,
6H), 2.09-2.21 (m, 1H), 3.61 (s, 3H), 3.63 (s, 6H), 3.98 (s, 3H),
4.04 (d, J=5.2 Hz, 1H), 6.30 (s, 2H), 7.35 (d, J=9.0 Hz, 1H), 7.69
(dd, J=2.2, 9.0 Hz, 1H), 8.18-8.34 (Br, 3H), 8.46 (d, J=2.2 Hz,
1H), 10.17-10.21 (Br, 1H).
EXAMPLE 67
N-((dimethylamino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5--
sulfonamide
[0499] A solution of Example 19 (1.00 g, 2.66 mmol) in
dichloromethane (25 mL) at room temperature was treated with DCC
(2.75 g, 13.3 mmol), 4-pyrrolidinylpyridine (0.20 g, 1.32 mmol) and
N,N-dimethylglycine (0.68 g, 6.40 mmol), stirred for 16 hours,
diluted with dichloromethane, and filtered. The filtrate was washed
with water, dried (MgSO.sub.4), filtered, and concentrated. The
concentrate was treated with dichloromethane, filtered, and
concentrated. The concentrate was purified by flash column
chromatography on silica gel with 1.5% methanol/dichloromethane,
dissolved in dichloromethane (5 mL) and diethyl ether (5 mL),
treated with 4M HCl in dioxane (0.55 mL), stirred for 10 minutes,
treated with ether, and filtered to provide the desired
product.
[0500] mp: 200-203.degree. C.; MS (ESI(+)) m/z 462 (M+H).sup.+;
.sup.1H NMR (DMSO-d.sub.6): .delta. 8.36 (d, J=1.8 Hz, 1H), 7.78
(dd, J.sub.1=8.7 Hz, J.sub.2=1,8 Hz, 1H), 7.73 (d, J=8.7 Hz, 1H),
7.61 (d, J=3.0 Hz, 1H), 6.76 (d, J=3.0 Hz, 1H), 6.73 (s, 2H), 3.92
(s, 2H), 3.82 (s, 3H), 3.82 (s, 6H), 3.75 (s, 3H), 2.67 (s, 6H);
Anal. calcd. for C.sub.22H.sub.27N.sub.3O.sub.6S.HCl.1.5H.sub.2O:
C, 50.43; H, 5.77; N, 8.02. Found: C, 50.50; H, 5.93; N, 8.01.
EXAMPLE 68
1-methyl-N-(((2S)-1-methylpyrrolidinyl)carbonyl)-N-(3,4,5-trimethoxyphenyl-
)-1H-indole-5-sulfonamide
[0501] The desired product was prepared by substituting
N-methyl-L-proline for N,N-dimethylglycine in Example 67.
[0502] MS (APCI(+)) m/z 488 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.36 (d, J=2 Hz, 1H), 7.75 (m, 2H), 7.61 (d, J=3 Hz, 1H),
6.82 (s, 2H), 4.76 (d, J=3 Hz, 1H), 4.06 (m, 1H), 3.90 (s, 3H),
3.81 (s, 6H), 3.77 (s, 3H), 3.00 (m, 2H), 2.69 (s, 3H), 1.90 (m,
3H), 1.73 (m, 1H).
EXAMPLE 69
N-((2S)-2-(dimethylamino)-3-methylbutanoyl)-1-methyl-N-(3.4,5-trimethoxyph-
enyl)-1H-indole-5-sulfonamide
[0503] The desired product was prepared by substituting
N,N-dimethyl-L-valine for N,N-dimethylglycine in Example 67.
[0504] MS (ESI(+)) m/z 504 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.38 (s, 1H), 7.75 (m, 2H), 7.60 (d, J=3 Hz, 1H), 6.76 (d,
J=3 Hz, 1H), 6.55 (br s, 2H), 3.89 (s, 3H), 3.82 (m, 1H), 3.79 (s,
9H), 2.68 (m, 3H), 2.55 (br s, 3H), 2.26 (br s, 1H), 0.91 (m, 3H),
0.75 (m, 3H).
EXAMPLE 70
N-((2S)-2-amino-3-methylbutanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-i-
ndole-5-sulfonamide
[0505] A solution of Example 19 (400 mg, 1.06 mmol) in
dichloromethane (8 mL) at room temperature was treated with DCC
(482 mg, 2.42 mmol), 4-pyrrolidinopyridine (16 mg, 0.11 mmol), and
N-(tert-butoxycarbonyl)-L-v- aline (462 mg, 2.13 mmol), stirred for
16 hours, treated with additional DCC (50 mg, 0.25 mmol) and
N-(tert-butoxycarbonyl)-L-valine (50 mg, 0.23 mmol), stirred for 4
hours, and filtered. The filtrate was treated with dichloromethane
(50 mL), washed with water, dried (Na.sub.2SO.sub.4), filtered, and
concentrated. The concentrate was purified by flash column
chromatography on silica gel with 2% methanol/dichloromethane,
dissolved in dioxane (5 mL), treated with 4M HCl in dioxane,
stirred for 2 hours, treated with ether (75 mL), filtered, and
dried to provide the desired product.
[0506] MS (ESI(+)) m/z 576 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.33 (m, 1H), 7.73 (m, 2H), 7.60 (d, J=3 Hz, 1H), 6.75 (d,
J=3 Hz, 1H), 6.65 (br s, 2H), 3.80 (br s, 7H), 3.76 (s, 3H), 3.57
(s, 3H), 2.10 (m, 1H), 0.80 (d, J=2.4 Hz, 3H), 0.78 (d, J=2.7 Hz,
3H).
EXAMPLE 71
1-methyl-N-((2S)-2-(methylamino)propanoyl)-N-(3,4,5-trimethoxyphenyl)-1H-i-
ndole-5-sulfonamide
[0507] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-N-methylalanine for
N-(tert-butoxycarbonyl)-L-v- aline in Example 70.
[0508] MS (DCI/NH.sub.3) m/z 462 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6) .delta. 8.95 (br s, 2H), 8.33 (m, 1H), 7.68-7.78 (m,
2H), 7.60 (d, J=4 Hz, 1H), 6.72 (d, J=4 Hz, 1H), 6.70 (br s, 2H),
3.89 (s, 3H), 3.81 (s, 6H), 3.77 (s, 3H), 3.62-3.71 (m, 1H), 2.37
(s, 3H), 1.38 (d, J=8 Hz, 3H).
EXAMPLE 72
N-((2R)-2-amino-2-phenylethanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-i-
ndole-5-sulfonamide
[0509] The desired product was prepared by substituting
(S)-N-(tert-butoxycarbonyl)-2-phenylglycine for
N-(tert-butoxycarbonyl)-L- -valine in Example 70.
[0510] MS (ESI(+)) m/z 510 (M+H).sup.+; 532 (M+Na).sup.+; .sup.1H
NMR (DMSO-d.sub.6) .delta. 8.52 (br s, 3H), 8.34 (m, 1H), 7.74 (s,
1H), 7.63(d, J=3.4 Hz, 1H), 7.41-7.27 (m, 3H), 6.83-6.67 (m, 4H),
5.51 (br s, 1H), 4.92 (s, 1H), 3.91 (s, 3H), 3.87-3.85 (m, 3H),
3.73 (s, 3H), 3.57 (s, 3H).
EXAMPLE 73
N-((2S)-2-amino-3-phenylpropanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H--
indole-5-sulfonamide
[0511] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-phenylalanine for
N-(tert-butoxycarbonyl)-L-val- ine in Example 70.
[0512] MS (ESI(+)) m/z 524 (M+H).sup.+; 546 (M+Na).sup.+; .sup.1H
NMR (DMSO-d.sub.6) .delta. 8.44 (br s, 3H), 8.32 (m, 1H), 7.74 (d,
J=1.6 Hz, 1H), 7.63 (d, J=3.4 Hz, 1H), 7.28-7.11 (m, 3H), 6.82-6.77
(m, 4H), 6.00 (br s, 11), 3.91 (s, 3H), 3.72-3.67 (m, 9H).
EXAMPLE 74
1-methyl-N-((2S)-pyrrolidinylcarbonyl)-N-(3,4,5-trimethoxyphenyl)-1H-indol-
e-5-sulfonamide
[0513] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-proline for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0514] MS (ESI(+)) m/z 474 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.35 (m, 1H), 7.76 (m, 2H), 7.61 (d, J=3 Hz, 1H), 6.75 (d,
J=3 Hz, 1H), 6.70 (br s, 2H), 4.03 (m, 1H), 3.89 (s, 3H), 3.81 (br
s, 6H), 3.76 (s, 3H), 3.20 (m, 2H), 1.95-1.75 (m, 4H).
EXAMPLE 75
N-((2S)-2,6-diaminohexanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-
-5-sulfonamide
[0515] The desired product was prepared by substituting
N,N-di-(tert-butoxycarbonyl)-L-lysine for
N-(tert-butoxycarbonyl)-L-valin- e in Example 70.
[0516] MS (ESI(+)) m/z 505 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.30 (m, 1H), 7.73 (m, 2H), 7.60 (d, J=3 Hz, 1H), 6.75 (d,
J=3 Hz, 1H), 6.65 (br s, 2H), 3.89 (s, 3H), 3.80 (m, 6H), 3.76 (s,
3H), 3.59 (m, 1H), 2.67 (m, 2H), 1.75-1.20 (m, 6H).
EXAMPLE 76
N-((2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl)-1-methyl-N-(3,4,5-trimethox-
yphenyl)-1H-indole-5-sulfonamide
[0517] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-histidine for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0518] mp: 175-177.degree. C.; MS (ESI(+)) m/z 514 (M+H).sup.+;
.sup.1H NMR (DMSO-d.sub.6) .delta.9.03 (s, 1H), 8.29 (s, 1H), 7.71
(m, 2H), 7.61 (d, J=3 Hz, 1H), 7.28 (s, 1H), 6.75 (d, J=3 Hz, 1H),
6.70 (br s, 2H), 4.01 (m, 1H), 3.89 (s, 3H), 3.79 (br s, 6H), 3.76
(s, 3H), 1.72 (m, 2H).
EXAMPLE 77
(2S)-2-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)an-
ilino)butanoic acid
[0519] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-aspartic acid 1-tert-butyl ester for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0520] mp: 156-159.degree. C.; MS (ESI(+)) m/z 492 (M+H).sup.+;
.sup.1H NMR (DMSO-d.sub.6) .delta. 8.35 (d, J=2 Hz), 7.75 (m, 2H),
7.60 (d, J=1H), 6.73 (d, J=3 Hz, 1H), 6.63 (br s, 2H), 4.01 (m,
1H), 3.88 (s, 3H), 3.82 (s, 6H), 3.76 (s, 3H), 2.80-2.70 (m,
2H).
EXAMPLE 78
(3S)-3-amino-4-oxo-4-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)an-
ilino)butanoic acid
[0521] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-aspartic acid 4-tert-butyl ester for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0522] MS (ESI(+)) m/z 492 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6):
.delta. 8.33 (m, 1H), 7.73 (m, 2H), 7.61 (d, J=3 Hz, 1H), 6.62 (d,
J=3 Hz, 1H), 6.40 (s, 2H), 3.95 (m, 1H), 3.88 (s, 3H), 3.76 (s,
6H), 3.74 (s, 3H), 2.75 (m, 2H).
EXAMPLE 79
(2R)-2-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)an-
ilino)pentanoic acid
[0523] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-glumatic acid 1-tert-butyl ester for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0524] MS (ESI(+)) m/z 506 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.31 (br s, 1H), 7.75 (dd, J.sub.1=9 Hz, J.sub.2=2 Hz, 1H),
7.69 (d, J=9 Hz, 1H), 7.58 (d, J=3 Hz, 1H), 6.72 (d, J=3 Hz, 1H),
6.69 (s, 1H), 6.66 (s, 1H), 4.06 (m, 1H), 3.88 (s, 3H), 3.80 (s,
6H), 3.72 (s, 3H), 2.30 (m, 1H), 2.22 (m, 1H), 1.95 (m, 1H), 1.80
(m, 1H).
EXAMPLE 80
(4S)-4-amino-5-oxo-5-(3,4,5-trimethoxy((1-methyl-1H-indol-5-yl)sulfonyl)an-
ilino)pentanoic acid
[0525] The desired product was prepared by substituting
N-(tert-butoxycarbonyl)-L-glumatic acid 5-tert-butyl ester for
N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0526] MS (ESI(+)) m/z 506 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta. 8.33 (m, 1H), 7.74 (m, 2H), 7.60 (d, J=3 Hz, 1H), 6.75 (d,
J=3 Hz, 1H), 6.71 (br s, 2H), 4.06 (m, 1H), 3.89 (s, 3H), 3.80 (s,
6H), 3.75 (s, 3H), 3.72 (s, 3H), 2.19 (m, 2H), 1.95-1.70 (m,
2H).
EXAMPLE 81
N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1-
H-indole-5-sulfonamide
EXAMPLE 81A
benzyl (bis(2-methoxyethyl)amino)acetate
[0527] A solution of benzyl bromoacetate (28.9 g; 96.7 mmol) in
dichloromethane (100 mL) at room temperature was treated with
2-methoxy-N-(2-methoxyethyl)ethanamine (40.6 g; 305 mmol), stirred
for 30 minutes, diluted with dichloromethane, washed sequentially
with saturated NH.sub.4Cl, water, and brine, dried
(Na.sub.2SO.sub.4), filtered, and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 40%
diethyl ether/dichloromethane to provide the desired product.
EXAMPLE 81B
(bis(2-methoxyethyl)amino)acetic acid
[0528] A solution of Example 81A (19.4 g; 69 mmol) and 10% Pd/C
(3.5 g) in methanol (150 mL) at room temperature was stirred under
4 atm of H.sub.2 for 17 hours, filtered through diatomaceous earth
(Celite.RTM.), and concentrated to provide the desired product.
EXAMPLE 81C
N-((bis(2-methoxyethyl)amino)acetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1-
H-indole-5-sulfonamide
[0529] The desired product was prepared by substituting Example 81B
for N,N-dimethylglycine in Example 67.
[0530] MS (DCI/NH.sub.3) m/z 550 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6) .delta. 8.29 (d, J=2 Hz, 1H), 7.77-7.68 (m, 2H),
7.57 (d, J=4 Hz, 1H), 6.73 (d, J=4 Hz, 1H), 6.61 (s, 2H), 3.88 (s,
3H), 3.78 (s, 6H), 3.72 (s, 3H), 3.19 (s, 2H), 3.16 (t, J=6 Hz,
4H), 3.04 (s, 6H), 2.61 (t, J=6 Hz, 4H); Anal. calcd. for
C.sub.26H.sub.36ClN.sub.3O.sub.8S: C, 53.28; H, 6.19; N, 7.17; Cl,
6.05. Found: C, 53.28; H, 6.03; N, 7.10; Cl, 5.97.
EXAMPLE 82
1-methyl-N-(4-morpholinylacetyl)-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-su-
lfonamide
[0531] The desired product was prepared by substituting morpholine
for 2-methoxy-N-(2-methoxyethyl)ethanamine in Example 81.
[0532] MS (DCI/NH.sub.3) m/z 504 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6) .delta. 8.29-7.77 (d, J=2 Hz, 1H), 7.68 (m, 2H),
7.57 (d, J=4 Hz, 1H), 6.73 (d, J=4 Hz, 1H), 6.63 (s, 2H), 3.88 (s,
3H), 3.80 (s, 6H), 3.72 (s, 3H), 3.47-3.41 (m, 4H), 2.94 (s, 2H),
2.31-2.27 (m, 4H).
EXAMPLE 83
1-methyl-N-((4-methyl-1-piperazinyl)acetyl)-N-(3,4,5-trimethoxyphenyl)-1H--
indole-5-sulfonamide
[0533] The desired product was prepared by substituting
N-methylpiperazine for 2-methoxy-N-(2-methoxyethyl)ethanamine in
Example 81.
[0534] MS (DCI/NH.sub.3) m/z 517 (M+H).sup.+; .sup.1H NMR
(DMSO-d.sub.6) .delta. 8.29 (d, J=2 Hz, 1H), 7.77-7.68 (m, 2H),
7.57 (d, J=4 Hz, 1H), 6.73 (d, J=4 Hz, 1H), 6.61 (s, 2H), 3.88 (s,
3H), 3.78 (s, 6H), 3.72 (s, 3H), 2.91 (s, 2H), 2.29-2.13 (m, 8H),
2.07 (s, 3H).
EXAMPLE 84
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-
-sulfonamide
EXAMPLE 84A
4-(((tert-butoxycarbonyl)amino)methyl)benzoic acid
[0535] A solution of 4-(aminomethyl)benzoic acid (1.51 g, 10 mmol),
di(tert-butyl) dicarbonate (2.62 g, 12 mmol), and sodium hydroxide
(0.48 g, 12 mmol) in tert-butanol (20 mL) was stirred for 16 hours,
treated with water (200 mL), and extracted with hexanes. The
aqueous layer was cooled to 5.degree. C., adjusted to pH 4 with 1M
NaHSO.sub.4, and extracted with ethyl acetate. The combined
extracts were dried (Na.sub.2SO.sub.4), filtered, and concentrated
to provide the desired product.
[0536] MS (ESI(+)) m/z 252 (M+H).sup.+, 269 (M+NH.sub.4).sup.+;
.sup.1H NMR (DMSO-d.sub.6) .delta. 8.06 (d, J=8.5 Hz, 2H), 7.38 (d,
J=8.5 Hz, 2H), 4.95 (br s, 1H), 4.40 (d, J=5.9 Hz, 2H), 1.48 (s,
9H).
EXAMPLE 84B
N-(4-(aminomethyl)benzoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-
-sulfonamide
[0537] The desired product was prepared by substituting Example 84A
for N-(tert-butoxycarbonyl)-L-valine in Example 70.
[0538] MS (ESI(+)) m/z 510 (M+H).sup.+; .sup.1H NMR (DMSO-d.sub.6)
.delta.8.35 (br s, 3H), 8.24 (m, 1H), 7.74 (s, 1H), 7.68(m, 2H),
7.59 (m, 3H), 7.34 (m, 2H), 6.73 (m, 1H), 6.57 (s, 2H), 3.95 (m,
2H), 3.88 (s, 3H), 3.64 (m, 6H), 3.56 (s, 3H).
EXAMPLE 85
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfanyl)benzene
EXAMPLE 85A
3,4,5-trimethoxybenzenethiol
[0539] A room temperature suspension of zinc powder (430 mg, 6.56
mmol) and dichlorodimethylsilane (0.80 mL, 6.59 mmol) in
1,2-dichloroethane (15 mL) was treated with a solution of
3,4,5-trimethoxybenzenesulfonyl chloride (500 mg, 1.87 mmol) and
1,3-dimethyl-2-imidazolidinone (647 mg, 5.67 mmol) in
1,2-dichloroethane (15 mL). The reaction was heated to 75.degree.
C. for 1 hour, cooled to room temperature, filtered, and
concentrated. The concentrate was dissolved in methanol,
concentrated, dissolved in methanol, and concentrated. The
concentrate was purified by flash column chromatography on silica
gel with dichloromethane to provide the desired product.
EXAMPLE 85B
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfanyl)benzene
[0540] A room temperature solution of Example 85A (194 mg, 0.97
mmol), 1-(chloromethyl)-4-methoxybenzene (162 mg, 1.03 mmol), and
KOH (70 mg, 1.25 mmol) in DMF (5 mL) was stirred for 2 hours,
treated with saturated NH.sub.4Cl, and extracted with ethyl
acetate. The combined extracts were washed with brine, dried
(MgSO.sub.4), filtered, and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 25%
ethyl acetate/hexanes to provide the desired product.
[0541] mp 65-67.degree. C.; MS (DCI/NH.sub.3) m/e 321 (M+H).sup.+;
.sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 7.27 (d, J=9 Hz, 2H),
6.87 (d, J=9 Hz, 2H), 6.59 (s, 2H), 4.19 (s, 2H), 3.72 (s, 3H),
3.61 (s, 3H), 3.32 (s, 6H); Anal. calcd. for
C.sub.17H.sub.20O.sub.4S: C, 63.72; H, 6.29. Found: C, 63.71; H,
6.39.
EXAMPLE 86
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfinyl)benzene
[0542] A room temperature mixture of Example 85B (100 mg, 0.31
mmol), acetic anhydride (38 mg, 0.37 mmol), and silica gel (65 mg)
in dichloromethane (1.5 mL) was treated with 30% H.sub.2O.sub.2 (45
.mu.L), stirred for 16 hours, filtered, and washed with
dichloromethane. The filtrate was washed sequentially with 10%
Na.sub.2SO.sub.3, saturated NaHCO.sub.3, and brine, dried
(Na.sub.2SO.sub.4), filtered, and concentrated. The concentrate was
purified by flash column chromatography on silica gel with 1%
methanol/dichloromethane to provide the desired product.
[0543] mp 103-105.degree. C.; MS (DCI/NH.sub.3) m/e 337
(M+H).sup.+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 7.01 (d,
J=9 Hz, 2H), 6.87 (d, J=9 Hz, 2H), 6.74 (s, 2H), 4.08 (dd, J=8 Hz,
54 Hz, 2H), 3.72 (s, 3H), 3.68 (s, 3H), 3.32 (s, 6H); Anal. calcd.
for C.sub.17H.sub.20O.sub.5- S: C, 60.70; H, 5.99. Found: C, 60.77;
H, 5.82.
EXAMPLE 87
1,2,3-trimethoxy-5-((4-methoxybenzyl)sulfonyl)benzene
[0544] A solution of Example 85B (160 mg, 0.50 mmol) in acetone (3
mL) was treated with water (3 mL), NaHCO.sub.3 (500 mg), and
potassium peroxymonosulfate (OXONE.RTM.) (415 mg, 0.67 mmol),
stirred for 3 hours, diluted with water, treated with solid
Na.sub.2SO.sub.3, and extracted with ethyl acetate. The combined
extracts were washed with water and brine, dried (MgSO.sub.4),
filtered, and concentrated to provide the desired product.
[0545] mp 95-97.degree. C.; MS (DCI/NH.sub.3) m/e 370
(M+NH.sub.4).sup.+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
7.09 (d, J=9 Hz, 2H), 6.91 (s, 2H), 6.87 (d, J=9 Hz, 2H), 4.58 (s,
2H), 3.77 (s, 3H), 3.72 (s, 3H), 3.32 (s, 6H); Anal. calcd. for
C.sub.17H.sub.20O.sub.6S: C, 57.94; H, 5.72. Found: C, 57.95; H,
5.68.
EXAMPLE 88
1,2,3-trimethoxy-5-((1-(4-methoxyphenyl)-1-methylethyl)sulfonyl)benzene
[0546] A 0.degree. C. solution of Example 87 (105 mg, 0.30 mmol) in
THF (2 mL) was treated with 1.0M lithium hexamethyldisilazide in
THF (0.36 mL, 0.36 mmol), stirred for 15 minutes, treated with
iodomethane (80 mg, 0.56 mmol), stirred for 15 minutes, treated
with a second portion of 1.0M lithium hexamethyldisilazide in THF
(0.36 mL, 0.36 mmol), stirred for 15 minutes, treated with
iodomethane (80 mg, 0.56 mmol), stirred for 15 minutes, acidified
with 10% HCl, and extracted with ethyl acetate. The combined
extracts were washed with brine, dried (MgSO.sub.4), filtered, and
concentrated. The concentrate was purified by flash column
chromatography on silica gel with 30% ethyl acetate/hexanes to
provide the desired product.
[0547] MS (DCI/NH.sub.3) m/e 398 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 7.27 (d, J=9 Hz, 2H), 6.87 (d, J=9
Hz, 2H), 6.48 (s, 2H), 3.75 (s, 3H), 3.72 (s, 3H), 3.63 (s, 6H),
1.70 (s, 6H); Anal. calcd. for C.sub.19H.sub.24O.sub.6S: C, 59.98;
H, 6.36. Found: C, 59.71; H, 6.38.
EXAMPLE 89
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline
EXAMPLE 89A
1,2,3-trimethoxy-5-((4-methoxy-3-nitrobenzyl)sulfanyl)benzene
[0548] The desired product was prepared by substituting
4-(chloromethyl)-2-nitrophenyl methyl ether for
1-(chloromethyl)-4-methox- ybenzene in Example 95.
EXAMPLE 89B
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfanyl)methyl)aniline
[0549] A room temperature suspension of zinc dust (2.35 g) in
acetic acid (20 mL) was treated dropwise with a solution of Example
89A (100 mg, 0.27 mmol) and acetic acid (0.40 mL) in
dichloromethane (1 mL), stirred at room temperature for 30 minutes,
diluted with ethyl acetate (100 mL), filtered, and concentrated.
The concentrate was dissolved in ethyl acetate, washed with
saturated NaHCO.sub.3 and brine, dried (MgSO.sub.4), filtered, and
concentrated. The concentrate was purified by flash column
chromatography on silica gel with 5% ethyl acetate/dichloromethane
to provide the desired product.
[0550] MS (DCI/NH.sub.3) m/e 336 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 6.70-6.65 (m, 2H), 6.58 (s, 2H), 6.50 (m,
1H), 4.68 (s, 2H), 4.05 (s, 2H), 3.72 (s, 6H), 3.71 (s, 3H), 3.63
(s, 3H).
EXAMPLE 90
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline
EXAMPLE 90A
1,2,3-trimethoxy-5-((4-methoxy-3-nitrobenzyl)sulfinyl)benzene
[0551] The desired product was prepared by substituting Example 99A
for Example 95B in Example 96.
EXAMPLE 90B
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfinyl)methyl)aniline
[0552] A room temperature solution of Example 90A (75 mg, 0.20
mmol) in ethanol (5 mL) was treated with 10% Pd/C (10 mg), stirred
under a hydrogen atmosphere for 3 hours, heated to reflux, stirred
for 3 hours, cooled to room temperature, filtered through
diatomaceous earth (Celite.RTM.), and concentrated. The concentrate
was purified by flash column chromatography with 50% ethyl
acetate/dichloromethane to provide the desired product.
[0553] MS (DCI/NH.sub.3) m/e 352 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 6.80 (s, 2H), 6.71 (d, J=8 Hz, 1H), 6.50 (d,
J=3 Hz, 1H), 6.31 (dd, J=3 Hz, 8 Hz, 1H), 4.76 (s, 2H), 3.93 (dd,
J=15 Hz, 45 Hz, 2H), 3.79 (s, 6H), 3.73 (s, 3H), 3.70 (s, 3H);
Anal. calcd. for C.sub.17H.sub.21NO.sub.5S: C, 58.10; H, 6.02; N,
3.99. Found: C, 58.14; H, 5.92; N, 3.85.
EXAMPLE 91
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline
EXAMPLE 91A
1,2,3-trimethoxy-5-((4-methoxy-3-nitrobenzyl)sulfonyl)benzene
[0554] The desired product was prepared by substituting Example 89A
for Example 85B in Example 87.
EXAMPLE 91B
2-methoxy-5-(((3,4,5-trimethoxyphenyl)sulfonyl)methyl)aniline
[0555] A solution of Example 91A (490 mg, 1.23 mmol) in methanol
(10 mL) was treated with SnCl.sub.2.2H.sub.2O (1.39 g; 6.17 mmol),
heated to reflux for I hour, and concentrated. The concentrate was
partitioned between saturated NaHCO.sub.3 and ethyl acetate, and
the aqueous phase was extracted with ethyl acetate. The combined
extracts were dried (MgSO.sub.4), filtered, and concentrated. The
concentrate was purified by flash column chromatography on silica
gel with 10% ethyl acetate/dichloromethane to provide the desired
product.
[0556] MS (DCI/NH.sub.3) m/e 385 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 6.92 (s, 2H), 6.70 (d, J=8 Hz, 1H),
6.53 (d, J=3 Hz, 1H), 6.30 (dd, J=3 Hz, 8 Hz, 1H), 4.76 (s, 2H),
4.44 (s, 2H), 3.79 (s, 6H), 3.73 (s, 6H).
EXAMPLE 92
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)aniline
EXAMPLE 92A
1,2,3-trimethoxy-5-((1-(4-methoxy-3-nitrophenyl)-1-methylethyl)sulfonyl)be-
nzene
[0557] The desired product was prepared by substituting Example 91A
for Example 87 in Example 88.
EXAMPLE 92B
2-methoxy-5-(1-methyl-1-((3,4,5-trimethoxyphenyl)sulfonyl)ethyl)aniline
[0558] The desired product was prepared by substituting Example 92A
for Example 90A in Example 90B.
[0559] MS (DCI/NH.sub.3) m/e 413 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 6.72 (d, J=3 Hz, 1H), 6.69 (d, J=8
Hz, 1H), 6.50 (s, 2H), 6.44 (dd, J=3 Hz, 9 Hz, 1H), 4.71 (s, 2H),
3.76 (s, 3H), 3.72 (s, 3H), 3.64 (s, 6H), 1.63 (s, 6H); Anal.
calcd. for C.sub.19H.sub.25NO.sub.6S: C, 57.70; H, 6.37; N, 3.54.
Found: C, 57.56; H, 6.27; N, 3.60.
EXAMPLE 93
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfanyl)methyl)benzene
[0560] The desired product was prepared by substituting
4-methoxybenzenethiol and 5-(chloromethyl)-1,2,3-trimethoxybenzene
for Example 85A and 1-(chloromethyl)-4-methoxybenzene,
respectively, in Example 85B.
[0561] MS (DCI/NH.sub.3) m/e 321 (M+H).sup.+; .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.30 (d, J=8 Hz, 2H), 6.89 (d, J=8 Hz, 2H),
6.51 (s, 2H), 4.02 (s, 2H), 3.73 (s, 3H), 3.68 (s, 6H), 3.33 (s,
3H).
EXAMPLE 94
1,2,3-trimethoxy-5-(((4-methoxyphenyl)sulfonyl)methyl)benzene
[0562] The desired product was prepared by substituting Example 93
for Example 85B in Example 87.
[0563] MS (DCI/NH.sub.3) m/e 370 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta.7.62 (d, J=8 Hz, 2H), 7.11 (d, J=8
Hz, 2H), 6.38 (s, 2H), 4.51 (s, 2H), 3.85 (s, 3H), 3.63 (s, 3H),
3.61 (s, 6H).
EXAMPLE 95
1,2,3-trimethoxy-5-(1-((4-methoxyphenyl)sulfonyl)-1-methylethyl)benzene
[0564] The desired product was prepared by substituting Example 94
for Example 87 in Example 88.
[0565] MS (DCI/NH.sub.3) m/e 398 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 7.31 (d, J=8 Hz, 2H), 7.03 (d, J=8
Hz, 2H), 6.51 (s, 2H), 3.82 (s, 3H), 3.67 (s, 3H), 3.65 (s, 6H),
1.69 (s, 6H).
EXAMPLE 96
2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline
EXAMPLE 96A
sodium 4-methoxy-3-nitrobenzenesulfinate
[0566] A solution of Na.sub.2SO.sub.3 (400 mg, 3.2 mmol) and
NaHCO.sub.3 (270 mg, 3.2 mmol) in H.sub.2O (10 mL) was treated
slowly with a solution of 4-methoxy-3-nitrobenzenesulfonyl chloride
(400 mg, 1.6 mmol) in acetone (3 mL), heated to 50.degree. C. for 2
hours, cooled to room temperature, and washed with dichloromethane.
The aqueous wash was filtered through cotton, lyophilized,
suspended in methanol, filtered, concentrated, and dried under
vacuum to provide the desired product.
EXAMPLE 96B
1,2,3-trimethoxy-5-(((4-methoxy-3-nitrophenyl)sulfonyl)methyl)benzene
[0567] A suspension of Example 96A (380 mg, 1.6 mmol) in DMF (10
mL) was treated slowly with a solution of
5-(chloromethyl)-1,2,3-trimethoxybenzen- e (344 mg, 1.6 mmol) in
DMF (5 mL), heated to 120.degree. C. for hour, cooled to room
temperature, and partitioned between ethyl acetate and brine. The
aqueous layer was extracted with ethyl acetate, and the combined
extracts were washed with water and brine, dried (MgSO.sub.4),
filtered, and concentrated. The concentrate was purified by flash
column chromatography on silica gel with 10% ethyl
acetate/dichloromethane to provide the desired product.
EXAMPLE 96C
2-methoxy-5-((3,4,5-trimethoxybenzyl)sulfonyl)aniline
[0568] The desired product was prepared by substituting Example 96B
for Example 90A in Example 90B.
[0569] MS (DCI/NH.sub.3) m/e 385 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 6.98-6.85 (m, 3H), 6.37 (s, 2H),
5.21 (s, 2H), 4.38 (s, 2H), 3.84 (s, 3H), 3.63 (s, 9H); Anal.
calcd. for C.sub.17H.sub.21NO.sub.6S: C, 55.57; H, 5.76; N, 3.81.
Found: C, 55.52; H, 5.77; N, 3.49.
EXAMPLE 97
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)aniline
EXAMPLE 97A
1
2,3-trimethoxy-5-(1-((4-methoxy-3-nitrophenyl)sulfonyl)-1-methylethyl)be-
nzene
[0570] The desired product was prepared by substituting Example 96B
for Example 87 in Example 88.
EXAMPLE 97B
2-methoxy-5-((1-methyl-1-(3,4,5-trimethoxyphenyl)ethyl)sulfonyl)aniline
[0571] The desired product was prepared by substituting Example 97A
for Example 90A in Example 90B.
[0572] MS (DCI/NH.sub.3) m/e 413 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 6.87 (d, J=8 Hz, 1H), 6.78 (d, J=8
Hz, 1H), 6.59 (dd, J=3 Hz, 8 Hz, 1H), 6.51 (s, 2H), 5.13 (s, 2H),
3.82 (s, 3H), 3.67 (s, 9H), 1.69 (s, 6H).
EXAMPLE 98
1,2,3-trimethoxy-5-((phenylsulfonyl)methyl)benzene
[0573] The desired product was prepared by substituting sodium
benzenesulfinate for Example 96A in Example 96B.
[0574] MS (DCI/NH.sub.3) m/e 340 (M+NH.sub.4).sup.+; .sup.1H NMR
(300 MHz, DMSO-d.sub.6) .delta. 7.72-7.57 (m, 5H), 6.38 (s, 2H),
4.58 (s, 2H), 3.64 (s, 3H), 3.40 (s, 6H).
[0575] Following Scheme 1 and the examples described above, the
following compounds can be prepared:
EXAMPLE 99
N-(2-aminoacetyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfonam-
ide
EXAMPLE 100
N-(2-aminoacetyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesulfon-
amide
EXAMPLE 101
N-((2S)-2-aminopropanoyl]-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5--
sulfonamide
EXAMPLE 102
N-((2S)-2-aminopropanoyl-1-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benz-
enesulfonamide
EXAMPLE 103
N-(3-aminopropanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-indole-5-sulfo-
namide
EXAMPLE 104
N-(3-aminopropanoyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol-5-yl)benzenesul-
fonamide
EXAMPLE 105
(2S)-2-amino-N-((1S)-1-methyl-2-oxo-2-(3,4,5-trimethoxy((1-methyl-1H-indol-
-5-yl)sulfonyl)anilino)ethyl)propanamide
EXAMPLE 106
(2S)-2-amino-N-((1S)-1-methyl-2-((1-methyl-1H-indol-5-yl)((3,4,5-trimethox-
yphenyl)sulfonyl)amino)-2-oxoethyl)propanamide
EXAMPLE 107
N-((2S)-2-amino-3-hydroxypropanoyl)-1-methyl-N-(3,4,5-trimethoxyphenyl)-1H-
-indole-5-sulfonamide
EXAMPLE 108
N-((2S)-2-amino-3-hydroxypropanoyl)-3,4,5-trimethoxy-N-(1-methyl-1H-indol--
5-yl)benzenesulfonamide
* * * * *