Novel method of diagnosing, monitoring, staging, imaging and treating various cancers

Abstract

The present invention provides a new method for detecting, diagnosing, monitoring, staging, prognosticating, imaging and treating selected cancers including gynecologic cancers such as breast, ovarian, uterine and endometrial cancer and lung cancer.

Description

FIELD OF THE INVENTION

[0001] This invention relates, in part, to newly developed assays for detecting, diagnosing, monitoring, staging, prognosticating, imaging and treating various cancers, particularly gynecologic cancer including ovarian, uterine endometrial and breast cancer, and lung cancer.

BACKGROUND OF THE INVENTION

[0002] The American Cancer Society has estimated that over 560,000 Americans will die this year from cancer. Cancer is the second leading cause of death in the United States, exceeded only by heart disease. It has been estimated that over one million new cancer cases will be diagnosed in 1999 alone.

[0003] In women; gynecologic cancers account for more than one-fourth of the malignancies.

[0004] Of the gynecologic cancers, breast cancer is the most common. According to the Women's Cancer Network, 1 out of every 8 women in the United States is as risk of developing breast cancer, and 1 out of every 28 women are at risk of dying from breast cancer. Approximately 77% of women diagnosed with breast cancer are over the age of 50. However, breast cancer is the leading cause of death in women between the ages of 40 and 55.

[0005] Carcinoma of the ovary is another very common gynecologic cancer. Approximately one in 70 women will develop ovarian cancer during her lifetime. An estimated 14,500 deaths in 1995 resulted from ovarian cancer. It causes more deaths than any other cancer of the female reproductive system. Ovarian cancer often does not cause any noticeable symptoms. Some possible warning signals, however, are an enlarged abdomen due to an accumulation of fluid or vague digestive disturbances (discomfort, gas or distention) in women over 40; rarely there will be abnormal vaginal bleeding. Periodic, complete pelvic examinations are important; a Pap test does not detect ovarian cancer. Annual pelvic exams are recommended for women over 40.

[0006] Also common in women is endometrial cancer or carcinoma of the lining of the uterus. According to the Women's Cancer Center endometrial cancer accounts for approximately 13% of all malignancies in women. There are about 34,000 cases of endometrial cancer diagnosed in the United States each year.

[0007] Uterine sarcoma is another type of uterine malignancy much more rare as compared to other gynecologic cancers. In uterine sarcoma, malignant cells start growing in the muscles or other supporting tissues of the uterus. Sarcoma of the uterus is different from cancer of the endometrium, a disease in which cancer cells start growing in the lining of the uterus. This uterine cancer usually begins after menopause. Women who have received therapy with high-dose X-rays (external beam radiation therapy) to their pelvis are at a higher risk to develop sarcoma of the uterus. These X-rays are sometimes given to women to stop bleeding from the uterus.

[0008] Lung cancer is the second most prevalent type of cancer for both men and women in the United States and is the most common cause of cancer death in both sexes. Lung cancer can result from a primary tumor originating in the lung or a secondary tumor which has spread from another organ such as the bowel or breast. Primary lung cancer is divided into three main types; small cell lung cancer non-small cell lung cancer; and mesothelioma. Small cell lung cancer is also called "Oat Cell" lung cancer because the cancer cells are a distinctive oat shape. There are three types of non-small cell lung cancer. These are grouped together because they behave in a similar way and respond to treatment differently to small cell lung cancer. The three types are squamous cell carcinoma, adenocarcinoma, and large cell carcinoma. Squamous cell cancer is the most common type of lung cancer. It develops from the cells that line the airways. Adenocarcinoma also develops from the cells that line the airways. However, adenocarcinoma develops from a particular type of cell that produces mucus (phlegm). Large cell lung cancer has been thus named because the cells look large and rounded when they are viewed under a microscope. Mesothelioma is a rare type of cancer which affects the covering of the lung called the pleura. Mesothelioma is often caused by exposure to asbestos.

[0009] Procedures used for detecting, diagnosing, monitoring, staging, and prognosticating each of these types of cancer are of critical importance to the outcome of the patient. In all cases, patients diagnosed early in development of the cancer generally have a much greater five-year survival rate as compared to the survival rate for patients diagnosed with a cancer which has metastasized. New diagnostic methods which are more sensitive and specific for early detection of various types of cancer are clearly needed.

[0010] In the present invention methods are provided for detecting, diagnosing, monitoring, staging, prognosticating, in vivo imaging and treating selected cancers including, but not limited to, gynecologic cancers such as ovarian, breast endometrial and/or uterine cancer, and lung cancer via detection of a Cancer Specific Genes (CSGs). Nine CGSs have been identified and refer, among other things, to native proteins expressed by the genes comprising the polynucleotide sequences of any of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 9. In the alternative, what is meant by the nine CSGs as used herein, means the native mRNAs encoded by the genes comprising any of the polynucleotide sequences of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 9 or it can refer to the actual genes comprising any of the polynucleotide sequences of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 9. Fragments of the CSGs such as those depicted in SEQ ID NO:10, 11, 12, 13 or 14 can also be detected.

[0011] Other objects, features, advantages and aspects of the present invention will become apparent to those of skill in the art from the following description. It should be understood, however, that the following description and the specific examples, while indicating preferred embodiments of the invention are given by way of illustration only. Various changes and modifications within the spirit and scope of the disclosed invention will become readily apparent to those skilled in the art from reading the following description and from reading the other parts of the present disclosure.

SUMMARY OF THE INVENTION

[0012] Toward these ends, and others, it is an object of the present invention to provide a method for diagnosing the presence of selected cancers by analyzing for changes in levels of CSG in cells, tissues or bodily fluids compared with levels of CSG in preferably the same cells, tissues, or bodily fluid type of a normal human control, wherein a change in levels of CSG in the patient versus the normal human control is associated with the selected cancer. For the purposes of this invention, by "selected dancer" it is meant to include gynecologic cancers such as ovarian, breast, endometrial and uterine cancer, and lung dancer.

[0013] Further provided is a method of diagnosing metastatic cancer in a patient having a selected cancer which is not known to have metastasized by identifying a human patient suspected of having a selected cancer that has metastasized; analyzing a sample of cells, tissues, or bodily fluid from such patient for CSG; comparing the CSG levels in such cells, tissues, or bodily fluid with levels of CSG in preferably the same cells, tissues, or bodily fluid type of a normal human control, wherein an increase in CSG levels in the patient versus the normal human control is associated with a cancer which has metastasized.

[0014] Also provided by the invention is a method of staging selected cancers in a human patient by identifying a human patient having such cancer; analyzing a sample of cells, tissues, or bodily fluid from such patient for CSG; comparing CSG levels in such cells, tissues, or bodily fluid with levels of CSG in preferably the same cells, tissues, or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the patient versus the normal human control is associated with a cancer which is progressing and a decrease in the levels of CSG is associated with a cancer which is regressing or in remission.

[0015] Further provided is a method of monitoring selected cancers in patients for the onset of metastasis. The method comprises identifying a human patient having a selected cancer that is not known to have metastasized; periodically analyzing a sample of cells, tissues, or bodily fluid from such patient for CSG; comparing the CSG levels in such cells, tissues, or bodily fluid with levels of CSG in preferably the same cells, tissues, or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the patient versus the normal human control is associated with a cancer which has metastasized.

[0016] Further provided is a method of monitoring the change in stage of selected cancers in humans having such cancer by looking at levels of CSG. The method comprises identifying a human patient having a selected cancer; periodically analyzing a sample of cells, tissues, or bodily fluid from such patient for CSG; comparing the CSG levels in such cells, tissue, or bodily fluid with levels of CSG in preferably the same cells, tissues, or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the patient versus the normal human control is associated with a cancer which is progressing and a decrease in the levels of CSG is associated with a cancer which is regressing or in remission.

[0017] Further provided are antibodies against CSG or fragments of such antibodies which can be used to detect or image localization of CSG in a patient for the purpose of detecting or diagnosing selected cancers. Such antibodies can be polyclonal or monoclonal, or prepared by molecular biology techniques. The term "antibody", as used herein and throughout the instant specification is also meant to include aptamers and single-stranded oligonucleotides such as those derived from an in vitro evolution protocol referred to as SELEX and well known to those skilled in the art. Antibodies can be labeled with a variety of detectable labels including, but not limited to, radioisotopes and paramagnetic metals. These antibodies or fragments thereof can also be used as therapeutic agents in the treatment of diseases characterized by expression of a CSG. In therapeutic applications, the antibody can be used without or with derivatization to a cytotoxic agent such as a radioisotope, enzyme, toxin, drug or a prodrug.

[0018] Other objects, features, advantages and aspects of the present invention will become apparent to those of skill in the art from the following description. It should be understood, however, that the following description and the specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only. Various changes and modifications within the spirit and scope of the disclosed invention will become readily apparent to those skilled in the art from reading the following description and from reading the other parts of the present disclosure.

DETAILED DESCRIPTION OF THE INVENTION

[0019] The present invention relates to diagnostic assays and methods, both quantitative and qualitative for detecting, diagnosing, monitoring, staging and prognosticating selected cancers by comparing levels of CSG with those of CSG in a normal human control. What is meant by levels of CSG as used herein is levels of the native protein expressed by the gene comprising the polynucleotide sequence of any of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 9. In the alternative, what is meant by levels of CSG as used herein is levels of the native mRNA encoded by the gene comprising any of the polynucleotide sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 9 or levels of the gene comprising any of the polynucleotide sequences of SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8 or 9. Fragments of CSGs such as those depicted in SEQ ID NO: 10, 11, 12, 13 and 14 can also be detected. Such levels are preferably measured in at least one of cells, tissues and/or bodily fluids, including determination of normal and abnormal levels. Thus, for instance, a diagnostic assay in accordance with the invention for diagnosing over-expression of CSG protein compared to normal control bodily fluids, cells, or tissue samples may be used to diagnose the presence of selected cancers. What is meant by "selected cancers" as used herein is a gynecologic cancer such as ovarian, breast, endometrial or uterine cancer, or lung case.

[0020] Any of the 9 CSGs can be measured alone in the methods of the invention, or all together or any combination thereof. However, for methods relating to gynecologic cancers including ovarian, breast, endometrial and uterine cancer, it is preferred that levels of CSG comprising SEQ ID NO:1 or a fragment thereof be determined. Exemplary fragments of this CSG which can be detected are depicted in SEQ ID NO: 10, 11, 12, and 13. For methods relating to lung cancer and gynecologic cancers including ovarian, endometrial and uterine, it is preferred that levels of CSG comprising SEQ ID NO:2 or 9 be determined. Fragments of this CSG such as that depicted in SEQ ID NO:14 can also be detected. For methods relating to ovarian cancer, determination of levels of CSG comprising SEQ ID NO:3 is also preferred.

[0021] All the methods of the present invention may optionally include measuring the levels of other cancer markers as well as CSG. Other cancer markers, in addition to CSG, useful in the present invention will depend on the cancer being tested and are known to those of skill in the art.

[0022] Diagnostic Assays

[0023] The present invention provides methods for diagnosing the presence of selected cancers by analyzing for changes in levels of CSG in cells, tissues or bodily fluids compared with levels of CSG in cells, tissues or bodily fluids of preferably the same type from a normal human control, wherein a change in levels of CSG in the patient versus the normal human control is associated with the presence of a selected cancer.

[0024] Without limiting the instant invention, typically, for a quantitative diagnostic assay a positive result indicating the patient being tested has cancer is one in which cells, tissues or bodily fluid levels of the cancer marker, such as CSG, are at least two times higher, and most preferably are at least five times higher, than in preferably the same cells, tissues or bodily fluid of a normal human control.

[0025] The present invention also provides a method of diagnosing metastases of selected cancers in a patient having a selected cancer which has not yet metastasized for the onset of metastasis. In the method of the present invention, a human cancer patient suspected of having a selected cancer which may have metastasized (but which was not previously known to have metastasized) is identified. This is accomplished by a variety of means known to those of skill in the art. For example, in the case of ovarian cancer patients are typically diagnosed with ovarian cancer following surgical staging and monitoring of CA125 levels. Traditional detection methods are also available and well known for other selected cancers which can be diagnosed by determination of CSG levels in a patient.

[0026] In the present invention, determining the presence of CSG levels in cells, tissues or bodily fluid, is particularly useful for discriminating between a selected cancer which has not metastasized and a selected cancer which has metastasized. Existing techniques have difficulty discriminating between cancers which have metastasized and cancers which have not metastasized and proper treatment selection is often dependent upon such knowledge.

[0027] In the present invention, the cancer marker levels measured in such cells, tissues or bodily fluid is CSG, and are compared with levels of CSG in preferably the same cells, tissue or bodily fluid type of a normal human control. That is, if the cancer marker being observed is CSG in serum, this level is preferably compared with the level of CSG in serum of a normal human patient. An increase in the CSG in the patient versus the normal human control is associated with a cancer which has metastasized.

[0028] Without limiting the instant invention, typically, for a quantitative diagnostic assay a positive result indicating the cancer in the patient being tested or monitored has metastasized is one in which cells, tissues or bodily fluid levels of the cancer marker, such as CSG, are at least two times higher, and most preferably are at least five times higher, than in preferably the same cells, tissues or bodily fluid of a normal patient.

[0029] Normal human control as used herein includes a human patient without cancer and/or non cancerous samples from the patient; in the methods for diagnosing or monitoring for metastasis, normal human control may also include samples from a human patient that is determined by reliable methods to have a selected cancer which has not metastasized.

[0030] Staging

[0031] The invention also provides a method of staging selected cancers in human patients. The method comprises identifying a human patient having a selected cancer and analyzing a sample of cells, tissues or bodily fluid from such human patient for CSG. Then, the method compares CSG levels in such cells, tissues or bodily fluid with levels of CSG in preferably the same cells, tissues or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the human patient versus the normal human control is associated with a cancer which is progressing and a decrease in the levels of CSG is associated with a cancer which is regressing or in remission.

[0032] Monitoring

[0033] Further provided is a method of monitoring selected cancers in humans for the onset of metastasis. The method comprises identifying a human patient having a selected cancer that is not known to have metastasized; periodically analyzing a sample of cells, tissues or bodily fluid from such human patient for CSG; comparing the CSG levels in such cells, tissues or bodily fluid with levels of CSG in preferably the same cells, tissues or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the human patient versus the normal human control is associated with a cancer which has metastasized.

[0034] Further provided by this invention is a method of monitoring the change in stage of selected cancers in humans having such cancers. The method comprises identifying a human patient having a selected cancer; periodically analyzing a sample of cells, tissues or bodily fluid from such human patient for CSG; comparing the CSG levels in such cells, tissues or bodily fluid with levels of CSG in preferably the same cells, tissues or bodily fluid type of a normal human control sample, wherein an increase in CSG levels in the human patient versus the normal human control is associated with a cancer which is progressing in stage and a decrease in the levels of CSG is associated with a cancer which is regressing in stage or in remission.

[0035] Monitoring such patient for onset of metastasis is periodic and preferably done on a quarterly basis. However, this may be more or less frequent depending on the cancer, the particular patient, and the stage of the cancer.

[0036] Assay Techniques

[0037] Assay techniques that can be used to determine levels of gene expression, such as CSG of the present invention, in a sample derived from a patient are well known to those of skill in the art. Such assay methods include radioimmunoassays, reverse transcriptase PCR (RT-PCR) assays, immunohistochemistry assays, in situ hybridization assays, competitive-binding assays, Western Blot analyses, ELISA assays and proteomic approaches. Among these, ELISAs are frequently preferred to diagnose a gene's expressed protein in biological fluids.

[0038] An ELISA assay initially comprises preparing an antibody, if not readily available from a commercial source, specific to CSG, preferably a monoclonal antibody. In addition a reporter antibody generally is prepared which binds specifically to CSG. The reporter antibody is attached to a detectable reagent such as radioactive, fluorescent or enzymatic reagent, for example horseradish peroxidase enzyme or alkaline phosphatase.

[0039] To carry out the ELISA, antibody specific to CSG is incubated on a solid support, e.g. a polystyrene dish, that binds the antibody. Any free protein binding sites on the dish are then covered by incubating with a non-specific protein such as bovine serum albumin. Next, the sample to be analyzed is incubated in the dish, during which time CSG binds to the specific antibody attached to the polystyrene dish. Unbound sample is washed out with buffer. A reporter antibody specifically directed to CSG and linked to horseradish peroxidase is placed in the dish resulting in binding of the reporter antibody to any monoclonal antibody bound to CSG. Unattached reporter antibody is then washed out. Reagents for peroxidase activity, including a calorimetric substrate are then added to the dish. Immobilized peroxidase, linked to CSG antibodies, produces a colored reaction product. The amount of color developed in a given time period is proportional to the amount of CSG protein present in the sample. Quantitative results typically are obtained by reference to a standard curve.

[0040] A competition assay may be employed wherein antibodies specific to CSG attached to a solid support and labeled CSG and a sample derived from the host are passed over the solid support and the amount of label detected attached to the solid support can be correlated to a quantity of CSG in the sample.

[0041] Nucleic acid methods may be used to detect CSG mRNA as a marker for selected cancers. Polymerase chain reaction (PCR) and other nucleic acid methods, such as ligase chain reaction (LCR) and nucleic acid sequence based amplification (NASABA), can be used to detect malignant cells for diagnosis and monitoring of the various selected malignancies. For example, reverse-transcriptase PCR (RT-PCR) is a powerful technique which can be used to detect the presence of a specific mRNA population in a complex mixture of thousands of other mRNA species. In RT-PCR, an mRNA species is first reverse transcribed to complementary DNA (cDNA) with use of the enzyme reverse transcriptase; the cDNA is then amplified as in a standard PCR reaction. RT-PCR can thus reveal by amplification the presence of a single species of mRNA. Accordingly, if the mRNA is highly specific for the cell that produces it, RT-PCR can be used to identify the presence of a specific type of cell.

[0042] Hybridization to clones or oligonucleotides arrayed on a solid support (i.e. gridding) can be used to both detect the expression of and quantitate the level of expression of that gene. In this approach, a cDNA encoding the CSG gene is fixed to a substrate. The substrate may be of any suitable type including but not limited to glass, nitrocellulose, nylon or plastic. At least a portion of the DNA encoding the CSG gene is attached to the substrate and then incubated with the analyte, which may be RNA or a complementary DNA (cDNA) copy of the RNA, isolated from the tissue of interest. Hybridization between the substrate bound DNA and the analyte can be detected and quantitated by several means including but not limited to radioactive labeling or fluorescence labeling of the analyte or a secondary molecule designed to detect the hybrid. Quantitation of the level of gene expression can be done by comparison of the intensity of the signal from the analyte compared with that determined from known standards. The standards can be obtained by in vitro transcription of the target gene, quantitating the yield, and then using that material to generate a standard curve.

[0043] Of the proteomic approaches, 2D electrophoresis is a technique well known to those in the art. Isolation of individual proteins from a sample such as serum is accomplished using sequential separation of proteins by different characteristics usually on polyacrylamide gels. First, proteins are separated by size using an electric current. The current acts uniformly on all proteins, so smaller proteins move farther on the gel than larger proteins. The second dimension applies a current perpendicular to the first and separates proteins not on the basis of size but on the specific electric charge carried by each protein. Since no two proteins with different sequences are identical on the basis of both size and charge, the result of a 2D separation is a square gel in which each protein occupies a unique spot. Analysis of the spots with chemical or antibody probes, or subsequent protein microsequencing can reveal the relative abundance of a given protein and the identity of the proteins in the sample.

[0044] The above tests can be carried out on samples derived from a variety of patients' cells, bodily fluids and/or tissue extracts (homogenates or solubilized tissue) such as from tissue biopsy and autopsy material. Bodily fluids useful in the present invention include blood, urine, saliva or any other bodily secretion or derivative thereof. Blood can include whole blood, plasma, serum or any derivative of blood.

[0045] In Vivo Antibody Use

[0046] Antibodies against CSG can also be used in vivo in patients suspected of suffering from a selected cancer including lung cancer or gynecologic cancers such as ovarian, breast, endometrial or uterine cancer. Specifically, antibodies against a CSG can be injected into a patient suspected of having a selected cancer for diagnostic and/or therapeutic purposes. The use of antibodies for in vivo diagnosis is well known in the art. For example, antibody-chelators labeled with Indium-111 have been described for use in the radioimmunoscintographic imaging of carcinoembryonic antigen expressing tumors (Sumerdbn et al. Nucl. Med. Biol. 1990 17: 247-254). In particular, these antibody-chelators have been used in detecting tumors in patients suspected of having recurrent colorectal cancer (Griffin et al. J. Clin. One. 1991 9: 631-640). Antibodies with paramagnetic ions as labels for use in magnetic resonance imaging have also been described (Lauffer, R. B. Magnetic Resonance in Medicine 1991 22: 339-342). Antibodies directed against CSGs can be used in a similar manner. Labeled antibodies against a CSG can be injected into patients suspected of having a selected cancer for the purpose of diagnosing or staging of the disease status of the patient. The label used will be selected in accordance with the imaging modality to be used. For example, radioactive labels such as Indium-111, Technetium-99m or Iodine-131 can be used for planar scans or single photon emission computed tomography (SPECT). Positron emitting labels such as Fluorine-19 can be used in positron emission tomography. Paramagnetic ions such as Gadlinium (III) or anganese (II) can used in magnetic resonance imaging (MRI). Localization of the label permits determination of the spread of the cancer. The amount of label within an organ or tissue also allows determination of the presence or absence of cancer in that organ or tissue.

[0047] For patients diagnosed with a selected cancer, injection of an antibody against a CSG can also have a therapeutic benefit. The antibody may exert its therapeutic effect alone. Alternatively, the antibody is conjugated to a cytotoxic agent such as a drug, toxin or radionuclide to enhance its therapeutic effect. Drug monoclonal antibodies have been described in the art for example by Garnett and Baldwin, Cancer Research 1986 46: 2407-2412. The use of toxins conjugated to monoclonal antibodies for the therapy of various cancers has also been described by Pastan et al. Cell 1986 47: 641-648 Yttrium-90 labeled monoclonal antibodies have been described for maximization of dose delivered to the tumor while limiting toxicity to normal tissues (Goodwin and Meares Cancer Supplement 1997 80: 2675-2680). Other cytotoxic radionuclides including, but not limited to Copper-67, Iodine-131 and Rhenium-186 can also be used for labeling of antibodies against CSGs.

[0048] Antibodies which can be used in these in vivo methods include both polyclonal and monoclonal antibodies and antibodies prepared via molecular biology techniques. Antibody fragments and aptamers and single-stranded oligonucleotides such as those derived from an in vitro evolution protocol referred to as SELEX and well known to those skilled in the art can also be used.

[0049] The present invention is further described by the following examples. These examples are provided solely to illustrate the invention by reference to specific embodiments. The exemplifications, while illustrating certain aspects of the invention, do not portray the limitations or circumscribe the scope of the disclosed invention.

EXAMPLES

Example 1

[0050] Identification of CSGs were carried out by a systematic analysis of data in the LIFESEQ database available from Incyte Pharmaceuticals, Palo Alto, Calif., using the data mining Cancer Leads Automatic Search Package (CLASP) developed by diaDexus LLC, Santa Clara, Calif.

[0051] The CLASP performs the following steps: selection of highly expressed organ specific genes based on the abundance level of the corresponding EST in the targeted organ versus all the other organs; analysis of the expression level of each highly expressed organ specific genes in normal, tumor tissue, disease tissue and tissue libraries associated with tumor or disease. Selection of the candidates demonstrating component ESTs were exclusively or more frequently found in tumor libraries. The CLASP allows the identification of highly expressed organ and cancer specific genes. A final manual in depth evaluation is then performed to finalize the CSGs selection.

1TABLE 1 CSG Sequences SEQ ID NO: Clone ID Gene ID 1 16656542 234617 2 1283171 332459 3 1649377 481154 4 236044H1 none assigned 5 none assigned 255687 6 none assigned 251313 7 none assigned 12029 8 none assigned 251804

[0052] The following examples are carried out using standard techniques, which are well known and routine to those of skill in the art, except where otherwise described in detail. Routine molecular biology techniques of the following example can be carried out as described in standard laboratory manuals, such as Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2nd Ed.; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989).

Example 2

Relative Quantitation of Gene Expression

[0053] Real-Time quantitative PCR with fluorescent Taqman probes is a quantitation detection system utilizing the 5'-3' nuclease activity of Taq DNA polymerase. The method uses an internal fluorescent oligonucleotide probe (Taqman) labeled with a 5' reporter dye and a downstream, 3' quencher dye. During PCR, the 5'-3' nuclease activity of Taq DNA polymerase releases the reporter, whose fluorescence can then be detected by the laser detector of the Model 7700 Sequence Detection System (PE Applied Biosystems, Foster-City, Calif., USA).

[0054] Amplification of an endogenous control is used to standardize the amount of sample RNA added to the reaction and normalize for Reverse Transcriptase (RT) efficiency. Either cyclophilin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or 18S ribosomal RNA (rRNA) is used as this endogenous control. To calculate relative quantitation between all the samples studied, the target RNA levels for one sample were used as the basis for comparative results (calibrator). Quantitation relative to the "calibrator" can be obtained using the standard curve method or the comparative method (User Bulletin #2: ABI PRISM 7700 Sequence Detection System).

[0055] The tissue distribution and the level of the target gene for every example in normal and cancer tissue were evaluated. Total RNA was extracted from normal tissues, cancer tissues, and from cancers and the corresponding matched adjacent tissues. Subsequently, first strand cDNA was prepared with reverse transcriptase and the polymerase chain reaction was done using primers and Taqman probe specific to each target gene. The results are analyzed using the ABI PRISM 7700 Sequence Detector. The absolute numbers are relative levels of expression of the target gene in a particular tissue compared to the calibrator tissue.

[0056] Measurement of Ovr110; Clone ID16656542; Gene ID 234617 (SEQ ID NO:1, 10, 11, 12 or 13)

[0057] The absolute numbers depicted in Table 2 are relative levels of expression of Ovr110 (SEQ ID NO:1 or a fragment thereof as depicted in SEQ ID NO:10, 11, 12, or 13) in 12 normal different tissues. All the values are compared to normal stomach (calibrator). These RNA samples are commercially available pools, originated by pooling samples of a particular tissue from different individuals.

2TABLE 2 Relative Levels of Ovr110 Expression in Pooled Samples Tissue NORMAL colon 0.00 endometrium 8.82 kidney 7.19 liver 0.36 ovary 1.19 pancreas 21.41 prostate 2.79 small intestine 0.03 spleen 0.00 00000000000000stoma 1.00 testis 8.72 uterus 0.93

[0058] The relative levels of expression in Table 2 show that Ovr110 is expressed at comparable levels in most of the normal tissues analyzed. Pancreas, with a relative expression level of 21.41, endometrium (8.82), testis (8.72), and kidney (7.19) are the only tissues expressing high levels of Ovr110 mRNA.

[0059] The absolute numbers in Table 2 were obtained analyzing pools of samples of a particular tissue from different individuals. They can not be compared to the absolute numbers originated from RNA obtained from tissue samples of a single individual in Table 3.

[0060] The absolute numbers depicted in Table 3 are relative levels of expression of Ovr110 in 73 pairs of matching samples. All the values are compared to normal stomach (calibrator). A matching pair is formed by mRNA from the cancer sample for a particular tissue and mRNA from the normal adjacent sample for that same tissue from the same individual. In addition, 15 unmatched cancer samples (from ovary and mammary gland) and 14 unmatched normal samples (from ovary and mammary gland) were also tested.

3TABLE 3 Relative Levels of Ovr110 Expression in Individual Samples Matching Normal Sample ID Tissue Cancer Adjacent Normal Ovr103X Ovary 1 86.22 0.53 Ovr1040O Ovary 2 168.31 Ovr1157 Ovary 3 528.22 Ovr63A Ovary 4 1.71 Ovr773O Ovary 5 464.65 Ovr1005O Ovary 6 18.32 Ovr1028 Ovary 7 7.78 Ovr1118 Ovary 8 0.00 Ovr130X Ovary 9 149.09 Ovr638A Ovary 10 3.14 OvrA1B Ovary 11 21.26 OvrA1C Ovary 12 1.83 OvrC360 Ovary 13 0.52 Ovr18GA Ovary 14 1.07 Ovr20GA Ovary 15 1.88 Ovr25GA Ovary 16 2.52 Ovr206I Ovary 17 2.51 Ovr32RA Ovary 18 3.01 Ovr35GA Ovary 19 5.17 Ovr40G Ovary 20 0.45 Ovr50GB Ovary 21 2.69 OvrC087 Ovary 22 0.47 OvrC179 Ovary 23 1.46 OvrC004 Ovary 24 4.99 OvrC007 Ovary 25 13.36 OvrC109 Ovary 26 6.61 MamS516 Mammary 16.39 13.74 Gland 1 MamS621 Mammary 826.70 4.60 Gland 2 MamS854 Mammary 34.60 18.30 Gland 3 Mam59X Mammary 721.57 27.00 Gland 4 MamS079 Mammary 80.73 5.10 Gland 5 MamS967 Mammary 6746.90 72.80 Gland 6 MamS127 Mammary 7.00 20.00 Gland 7 MamB011X Mammary 1042.00 29.00 Gland 8 Mam12B Mammary 1342.00 Gland 9 Mam82XI Mammary 507.00 Gland 10 MamS123 Mammary 24.85 4.24 Gland 11 MamS699 Mammary 84.74 5.54 Gland 12 MamS997 Mammary 482.71 11.84 Gland 13 Mam162X Mammary 15.73 10.59 Gland 14 MamA06X Mammary 1418.35 8.20 Gland 15 Mam603X Mammary 294.00 Gland 16 Mam699F Mammary 567.40 86.60 Gland 17 Mam12X Mammary 425.00 31.00 Gland 18 MamA04 Mammary 2.00 Gland 19 Mam42DN Mammary 46.05 31.02 Gland 20 Utr23XU Uterus 1 600.49 27.95 Utr85XU Uterus 2 73.52 18.83 Utr135XO Uterus 3 178.00 274.00 Utr141XO Uterus 4 289.00 26.00 CvxNKS54 Cervix 1 2.47 0.61 CvxKS83 Cervix 2 1.00 2.00 CvxNKS18 Cervix 3 1.00 0.00 CvxNK23 Cervix 4 5.84 14.47 CvxNK24 Cervix 5 20.32 33.13 End68X Endometrium 1 167.73 544.96 End8963 Endometrium 2 340.14 20.89 End8XA Endometrium 3 1.68 224.41 End65RA Endometrium 4 303.00 5.00 End8911 Endometrium 5 1038.00 74.00 End3AX Endometrium 6 6.59 1.69 End4XA Endometrium 7 0.43 15.45 End5XA Endometrium 8 17.81 388.02 End10479 Endometrium 9 1251.60 31.10 End12XA Endometrium 312.80 33.80 10 Kid107XD Kidney 1 2.68 29.65 Kid109XD Kidney 2 81.01 228.33 Kid10XD Kidney 3 0.00 15.30 Kid6XD Kidney 4 18.32 9.06 Kid11XD Kidney 5 1.38 20.75 Kid5XD Kidney 6 30.27 0.19 Liv15XA Liver 1 0.00 0.45 Liv42X Liver 2 0.81 0.40 Liv94XA Liver 3 12.00 2.16 Lng LC71 Lung 1 5.45 3.31 LngAC39 Lung 2 1.11 0.00 LngBR94 Lung 3 4.50 0.00 LngSQ45 Lung 4 15.03 0.76 LngC20X Lung 5 0.00 1.65 LngSQ56 Lung 6 91.77 8.03 ClnAS89 Colon 1 0.79 7.65 ClnC9XR Colon 2 0.03 0.00 ClnRC67 Colon 3 0.00 0.00 ClnSG36 Colon 4 0.81 0.35 ClnTX89 Colon 5 0.00 0.00 ClnSG45 Colon 6 0.00 0.06 ClnTX01 Colon 7 0.00 0.00 Pan77X Pancreas 1 0.89 2.62 Pan71XL Pancreas 2 3.99 0.12 Pan82XP Pancreas 3 59.92 28.44 Pan92X Pancreas 4 17.21 0.00 StoAC93 Stomach 1 7.54 6.43 StoAC99 Stomach 2 19.49 3.19 StoAC44 Stomach 3 3.62 0.37 SmI21XA Small 0.00 0.00 Intestine 1 SmIH89 Small 0.00 0.00 Intestine 2 Bld32XK Bladder 1 0.00 0.21 Bld46XK Bladder 2 0.36 0.32 BldTR17 Bladder 3 0.28 0.00 Tst39X Testis 11.24 2.24 Pro84XB Prostate 1 2.60 24.30 Pro90XB Prostate 2 1.40 2.00

[0061] 0.00=Negative

[0062] Table 2 and Table 3 represent a combined total of 187 samples in 16 different tissue types. In the analysis of matching samples, the higher levels of expression were in mammary gland, uterus, endometrium and ovary, showing a high degree of tissue specificity for the gynecologic tissues. Of all the samples different than those mentioned before analyzed, only a few samples (Kid109XD, LngSQ56, and Pan82XP) showed high levels of expression of Ovr110.

[0063] Furthermore, the level of mRNA expression was compared in cancer samples and the isogenic normal adjacent tissue from the same individual. This comparison provides an indication of specificity for the cancer stage (e.g. higher levels of mRNA expression in the cancer sample compared to the normal adjacent). Table 3 shows overexpression of Ovr110 in 15 of 16 mammary gland cancer tissues compared with their respective normal adjacent (mammary gland samples MamS516, MamS621, MamS854, Mam59X, MamS079, MamS967, MamB011X, MamS123, MamS699, MamS997, Mam162X, MamA06X, Mam699F, Mam12X, and Mam42DN). There was overexpression in the cancer tissue for 94% of the mammary gland matching samples tested.

[0064] For uterus, Ovr110 is overexpressed in 3 of 4 matching samples (uterus samples Utr23XU, Utr85XU, and Utr141XO). There was overexpression in the cancer tissue for 75% of the uterus matching samples analyzed.

[0065] For endometrium, Ovr110 is overexpressed in 6 of 10 matching samples (endometrium samples End8963, End65RA, End8911, End3AX, End10479, and End12XA). There was overexpression in the cancer tissue for 60% of the endometrium matching samples.

[0066] For ovary, Ovr110 shows overexpression in 1 of 1 matching sample. For the unmatched ovarian samples, 8 of 12 cancer samples show expression values of Ovr110 higher than the median (2.52) for the normal unmatched ovarian samples. There was overexpression in the cancer tissue for 67% of the unmatched ovarian samples.

[0067] Altogether, the level of tissue specificity, plus the mRNA overexpression in most of the matching samples tested are indicative of Ovr110 (including SEQ ID NO:1, 10, 11, 12 or 13) being a diagnostic marker for gynecologic cancers, specifically, mammary gland or breast, uterine, ovarian and endometrial cancer.

[0068] Measurement of Ovr114; Clone ID1649377; Gene ID 481154 (SEQ ID NO:3)

[0069] The numbers depicted in Table 4 are relative levels of expression in 12 normal tissues of Ovr114 compared to pancreas (calibrator). These RNA samples were obtained commercially and were generated by pooling samples from a particular tissue from different individuals.

4TABLE 4 Relative Levels of Ovr114 Expression in Pooled Samples Tissue Normal Colon 2.3 Endometrium 7.6 Kidney 0.5 Liver 0.6 Ovary 5.2 Pancreas 1.0 Prostate 2.1 Small Intestine 1.3 Spleen 2.4 Stomach 1.5 Testis 15.8 Uterus 8.8

[0070] The relative levels of expression in Table 4 show that Ovr114 mRNA expression is detected in all the pools of normal tissues analyzed.

[0071] The tissues shown in Table 4 are pooled samples from different individuals. The tissues shown in Table 5 were obtained from individuals and are not pooled. Hence the values for mRNA expression levels shown in Table 4 cannot be directly compared to the values shown in Table 5.

[0072] The numbers depicted in Table 5 are relative levels of expression of Ovr114 compared to pancreas (calibrator), in 46 pairs of matching samples and 27 unmatched tissue samples. Each matching pair contains the cancer sample for a particular tissue and the normal adjacent tissue sample for that same tissue from the same individual. In cancers (for example, ovary) where it was not possible to obtain normal adjacent samples from the same individual, samples from a different normal individual were analyzed.

5TABLE 5 Relative Levels of Ovr114 Expression in Individual Samples Normal & Borderline Matching Normal Tissue Sample ID Cancer Type Cancer Malignant Adjacent Ovary 1 Ovr1037O/1038O Papillary serous 17.04 3.93 adenocarcinoma, G3 Ovary 2 OvrG021SPI/SN2 Papillary serous 1.62 4.34 adenocarcinoma Ovary 3 OvrG010SP/SN Papillary serous 0.50 1.12 adenocarcinoma Ovary 4 OvrA081F/A082D Mucinous tumor, low 0.84 0.96 malignant potential Ovary 5 OvrA084/A086 Mucinous tumor, grade G-B, 5.24 6.00 borderline Ovary 6 Ovr14604A1C Serous cystadenofibroma, 5.33 low malignancy Ovary 7 Ovr14638A1C Follicular cysts, low 8.11 malignant potential Ovary 8 Ovr1040O Papillary serous 13.27 adenocarcinoma, G2 Ovary 9 Ovr1157O Papillary serous 106.08 adenocarcinoma Ovary 10 Ovr1005O Papillary serous 77.04 endometricarcinoma Ovary 11 Ovr1028O Ovarian carcinoma 14.78 Ovary 12 Ovr14603A1D Adenocarcinoma 22.23 Ovary 13 Ovr9410C360 Endometrioid 4.74 adenocarcinoma Ovary 14 Ovr1305X Papillary serous 96.49 adenocarcinoma Ovary 15 Ovr773O Papillary serous 8.40 adenocarcinoma Ovary 16 Ovr988Z Papillary serous 6.40 adenocarcinoma Ovary 17 Ovr9702C018GA Normal Cystic 12.06 Ovary 18 Ovr2061 Normal left atrophic, 10.11 small cystic Ovary 19 Ovr9702C020GA Normal-multiple ovarian 12.70 cysts Ovary 20 Ovr9702C025GA Normal-hemorrhage CL cysts 22.09 Ovary 21 Ovr9701C050GB Normal-multiple ovarian 9.01 cysts Ovary 22 Ovr9701C087RA Normal-small follicle 1.86 cysts Ovary 23 Ovr9702C032RA 7.81 Ovary 24 Ovr9701C109RA Normal 1.50 Ovary 25 Ovr9411C057R Benign large endometriotic 5.22 cyst Ovary 26 Ovr9701C179a Normal 3.09 Ovary 27 Ovr1461O Serous cystadenofibroma, 3.53 no malignancy Ovary 28 Ovr9701C035GA Normal 6.32 Ovary 29 Ovr9702C007RA Normal 0 Ovary 30 Ovr9701C087RA Normal-small follicle 1.97 cysts Ovary 31 Ovr9411C109 Normal 9.49 Ovary 32 Ovr9701C177a Normal-cystic follicles 3.85 Endometrium 1 End14863A1A/A2A Moderately differ. Endome. 1.30 0.70 carcinoma/NAT Endometrium 2 End9709C056A/55A Endometrial 1.83 11.90 adenocarcinoma/NAT Endometrium 3 End9704C281A/2A Endometrial 13.32 7.76 adenocarcinoma/NAT Endometrium 4 End9705A125A/6A Endometrial 3.62 3.34 adenocarcinoma/NAT Mammary Gland 1 Mam00042D01/N01 3.13 0.76 Mammary Gland 2 MamS99-522A/B 4.45 0.45 Mammary Gland 3 Mam1620F/1621F 0.74 1.91 Mammary Gland 4 Mam4003259a/g 3.48 2.00 Uterus 1 Utr850U/851U Stage 1 endometrial 46.96 11.96 cancer/NAT Uterus 2 Utr233U96/234U96 Adenocarcinoma/NAT 20.02 5.90 Uterus 3 Utr1359O/1358O Tumor/NAT 10.23 7.74 Uterus 4 Utr1417O/1418O Malignant tumor/NAT 7.52 4.92 Cervix 1 CvxVNM00083/83 Keratinizing squamous cell 5.47 14.31 carcinoma Cervix 2 CvxIND00023D/N Large cell nonkeratinizing 4.99 3.99 carcinoma Cervix 3 CvxIND00024D/N Large cell nonkeratinizing 10.14 14.22 carcinoma Bladder 1 Bld665T/664T 1.43 4.03 Bladder 2 Bld327K/328K Papillary transitional 1.15 0.99 cell carcinoma/NAT Kidney 1 Kid4003710C/F 0.03 0.35 Kidney 2 Kid1242D/1243D 1.61 0.14 Lung 1 Lng750C/751C Metastatic osteogenic 2.44 5.73 sarcoma/NAT Lung 2 Lng8890A/8890B Cancer/NAT 1.11 5.19 Lung 3 Lng9502C109R/10R 1.99 0.80 Liver 1 Liv1747/1743 Hepatocellular 0.67 1.07 carcinoma/NAT Liver 2 LivVNM00175/175 Cancer/NAT 15.46 2.85 Skin 1 Skn2S9821248A/B Secondary malignant 2.83 0.70 melanoma Skin 2 Skn4005287A1/B2 0.91 4.02 Small Int. 1 SmI9802H008/009 0.87 0.82 Stomach 1 Sto4004864A4/B4 Adenocarcinoma/NAT 0.81 1.22 Stomach 2 StoS9822539A/B Adenocarcinoma/NAT 1.22 1.39 Stomach 3 StoS99728A/C Malignant gastrointestinal 0.47 0.35 stromal tumor Prostate 1 Pro1012B/1013B Adenocarcinoma/NAT 2.39 2.61 Prostate 2 Pro1094B/1095B 0.10 0.38 Pancreas 1 Pan776p/777p Tumor/NAT 2.39 0.52 Pancreas 2 Pan824p/825p Cystic adenoma 1.66 1.22 Testis 1 Tst239X/240X Tumor/NAT 1.24 1.72 Colon 1 Cln9706c068ra/69ra Adenocarcinoma/NAT 0.38 0.65 Colon 2 Cln4004732A7/B6 Adenocarcinoma/NAT 0.44 1.26 Colon 3 Cln4004695A9/B8 1.94 1.53 Colon 4 Cln9612B006/005 Asc. Colon, Cecum, 3.38 1.10 adenocarcinoma Colon 5 Cln9704C024R/25R Adenocarcinoma/NAT 1.66 2.77

[0073] Table 4 and Table 5 represent a combined total of 129 samples in 17 human tissue types. Among 117 samples in Table 5 representing 16 different tissues high levels of expression are seen only in ovarian cancer samples. The median expression of Ovr114 is 14.03 (range: 0.5-106.08) in ovarian cancer and 4.34 (range: 0-22.09) in normal ovaries. In other words, the median expression levels of Ovr114 in cancer samples is increased 3.5 fold as compared with that of the normal ovarian samples. Five of 12 ovarian cancers (42%) showed increased expression relative to normal ovary (with 95% specificity). The median expression of Ovr114 in other gynecologic cancers is 4.99, and 2 out of 15 samples showed expression levels comparable with that in ovarian cancer. The median of the expression levels of Ovr114 in the rest of the cancer samples is 1.24, which is more than 11 fold less than that detected in ovarian cancer samples. No individual showed an expression level comparable to that of ovarian cancer samples (except Liver 2; LivVNM00175/175).

[0074] The 3.5 fold increase in expression in 42% of the individual ovarian cancer samples and no compatible expression in other non-gynecologic cancers is indicative of Ovr114 being a diagnostic marker for detection of ovarian cancer cells. It is believed that the Ovr114 marker may also be useful in detection of additional gynecologic cancers.

[0075] Measurement of Ovr115; Clone ID1283171; Gene ID 332459 (SEQ ID NO:2 or 14)

[0076] The numbers depicted in Table 6 are relative levels of expression Ovr115 compared to their respective calibrators. The numbers are relative levels of expression in 12 normal tissues of ovaries compared to Testis (calibrator). These RNA samples were obtained commercially and were generated by pooling samples from a particular tissue from different individuals.

6TABLE 6 Relative Levels of Ovr115 Expression in Pooled Samples Tissue Normal Colon 858.10 Endometrium 12.34 Kidney 3.76 Liver 0.00 Ovary 0.43 Pancreas 0.00 Prostate 8.91 Small Intestine 62.25 Spleen 0.00 Stomach 37.53 Testis 1.00 Uterus 47.67

[0077] The relative levels of expression in Table 6 show that Ovr115 mRNA expression is detected in all the 12 normal tissue pools analyzed.

[0078] The tissues shown in Table 6 are pooled samples from different individuals. The tissues shown in Table 7 were obtained from individuals and are not pooled. Hence the values for mRNA expression levels shown in Table 6 cannot be directly compared to the values shown in Table 7.

[0079] The numbers depicted in Table 7 are relative levels of expression of Ovr115 compared to testis (calibrator), in 46 pairs of matching samples and 27 unmatched tissue samples. Each matching pair contains the cancer sample for a particular tissue and the normal adjacent tissue sample for that same tissue from the same individual. In cancers (for example, ovary) where it was not possible to obtain normal adjacent samples from the same individual, samples from a different normal individual were analyzed.

7TABLE 7 Relative Levels of Ovr115 Expression in Individual Samples Normal & Borderline Matching Normal Tissue Sample ID Cancer Type Cancer Malignant Adjacent Ovary 1 Ovr1037O/1038O Papillary serous 193.34 0.24 adenocarcinoma, G3 Ovary 3 OvrG021SPI/SN2 Papillary serous 0.38 0.31 adenocarcinoma Ovary 4 OvrG010SP/SN Papillary serous 231.25 0.45 adenocarcinoma Ovary 2 OvrA084/A086 Mucinous tumor, grade G- 143.34 16.65 B, borderline Ovary 5 OvrA081F/A082D Mucinous tumor, low 314.13 0 malignant potential Ovary 19 Ovr14604A1C Serous cystadenofibroma, 299.87 low malignancy Ovary 26 Ovr14638A1C Follicular cysts, low 1278.32 malignant potential Ovary 6 Ovr1040O Papillary serous 144.25 adenocarcinoma, G2 Ovary 22 Ovr9410C360 Endometrioid 0.29 adenocarcinoma Ovary 23 Ovr1305X Papillary serous 157.41 adenocarcinoma Ovary 27 Ovr773O Papillary serous 340.04 adenocarcinoma Ovary 28 Ovr988Z Papillary serous 464.75 adenocarcinoma Ovary 7 Ovr1157O Papillary serous 432.07 adenocarcinoma Ovary 8 Ovr1005O Papillary serous 74.23 endometricarcinoma Ovary 9 Ovr1028O Ovarian carcinoma 1408.79 Ovary 10 Ovr14603A1D Adenocarcinoma 0.00 Ovary 11 Ovr9702C018GA Normal Cystic 0.16 Ovary 12 Ovr2061 Normal left atrophic, 0.00 small cystic Ovary 13 Ovr9702C020GA Normal-multiple ovarian 0.00 cysts Ovary 14 Ovr9702C025GA Normal-hemorrhage CL 0.00 cysts Ovary 15 Ovr9701C050GB Normal-multiple ovarian 0.91 cysts Ovary 16 Ovr9701C087RA Normal-small follicle 0.00 cysts Ovary 17 Ovr9702C032RA 0.28 Ovary 18 Ovr9701C109RA Normal 0.00 Ovary 20 Ovr9411C057R Benign large 38.87 endometriotic cyst Ovary 21 Ovr9701C179a Normal 0.08 Ovary 24 Ovr1461O Serous cystadenofibroma, 0.00 no malignancy Ovary 25 Ovr9701C035GA Normal 0.00 Ovary 29 Ovr9702C007RA Normal 0.00 Ovary 30 Ovr9701C087RA Normal-small follicle 0.00 cysts Ovary 31 Ovr9411C109 Normal 0.00 Ovary 32 Ovr9701C177a Normal-cystic follicles 0.00 Uterus 1 Utr850U/851U Stage 1 endometrial 39.95 13.60 cancer/NAT Uterus 2 Utr233U96/234U96 Adenocarcinoma/NAT 140.37 22.67 Uterus 3 Utr1359O/1358) Tumor/NAT 16.45 32.50 Uterus 4 Utr1417O/1418O Malignant tumor/NAT 288.52 5.29 Endometrium 1 End14863A1A/A2A Moderately differ. 2.61 6.24 Endome. carcinoma/NAT Endometrium 2 End9709C056A/55A Endometrial 2.10 49.40 adenocarcinoma/NAT Endometrium 3 End9704C281A/2A Endometrial 480.77 19.22 adenocarcinoma/NAT Endometrium 4 End9705A125A/6A Endometrial 322.07 31.08 adenocarcinoma/NAT Lung 1 Lng750C/751C Metastatic osteogenic 38.81 7.36 sarcoma/NAT Lung 2 Lng8890A/8890B Cancer/NAT 690.12 14.71 Lung 3 Lng9502C109R/10R 1756.90 2.86 Skin 1 Skn2S9821248A/B Secondary malignant 10.56 0.00 melanoma Skin 2 Skn4005287A1/B2 331.30 47.23 Prostate 1 Pro1012B/1013B Adenocarcinoma/NAT 14.64 4.39 Prostate 2 Pro1094B/1095B 0.09 2.54 Bladder 1 B1d665T/664T 404.56 90.20 Bladder 2 B1d327K/328K Papillary transitional 77.35 177.37 cell carcinoma/NAT Kidney 1 Kid4003710C/F 0.17 12.72 Kidney 2 Kid1242D/1243D 0.00 13.74 Mammary Gland 1 Mam1620F/1621F 0.27 0.12 Mammary Gland 2 Mam4003259a/g 5.71 0.00 Liver 1 Liv1747/1743 Hepatocellular 0.14 0.69 carcinoma/NAT Liver 2 LivVNM00175/175 Cancer/NAT 0.00 0.00 Small Int. 1 SmI9802H008/009 128.44 151.38 Stomach 1 Sto4004864A4/B4 Adenocarcinoma/NAT 303.01 116.72 Stomach 2 StoS9822539A/B Adenocarcinoma/NAT 24.12 17.76 Stomach 3 StoS99728A/C Malignant 0.00 9.10 gastrointestinal stromal tumor Pancreas 1 Pan776p/777p Tumor/NAT 0.00 0.43 Pancreas 2 Pan824p/825p Cystic adenoma 0.00 3.17 Testis 1 Tst239X/240X Tumor/NAT 24.05 1.37 Colon 1 Cln9706c068ra/69ra Adenocarcinoma/NAT 605.60 169.77 Colon 2 Cln4004732A7/B6 Adenocarcinoma/NAT 367.20 281.32 Colon 3 Cln4004695A9/B8 316.15 295.77 Colon 4 Cln9612B006/005 Asc. Colon. Cecum, 820.89 543.52 adenocarcinoma Colon 5 Cln9704C024R/25R Adenocarcinoma/NAT 161.18 150.07 Cervix 1 CvxVNM00083/83 Keratinizing squamous 738.17 1195.88 cell carcinoma Cervix 2 CvxIND00023D/N Large cell 1473.04 1229.80 nonkeratinizing carcinoma Cervix 3 CvxIND00024D/N Large cell 2877.48 1275.02 nonkeratinizing carcinoma

[0080] Table 6 and Table 7 represent a combined total of 129 samples in 17 human tissue types. Comparisons of the level of mRNA expression in ovarian cancer samples and the normal adjacent tissue from the same individuals or normal tissues from other individuals are shown in Table 7. Ovr115 was expressed at higher levels in 9 of 12 cancer tissues (75%), relative to the maximum level detected in all 21 normal or normal adjacent ovarian samples. All 4 of 4 (100%) ovarian tumors with borderline malignancy had elevated Ovr115 expression. The median expression in ovarian cancers (including the ones with borderline malignancy) was 212.30 while the median expression in normal ovaries was 0. When compared with their own normal adjacent tissue samples, expression levels of Ovr115 were also elevated in 3 of 3 (100%) lung cancers, 3 of 4 (75%) uterus cancers and 2 of 4 (50%) endometrial cancers.

[0081] The relatively high expression levels of Ovr115 in ovarian and other selected cancer samples is indicative of Ovr115 being a diagnostic marker for detection of ovarian, lung, uterine and endometrial cancer.

[0082] A homolog of Ovr115 has also been identified in public data base; g2597613 as gi.vertline.2507612.vertline.gb.vertline.U75329.1.vertline.HS- U75329 Human serine protease mRNA, complete CDS. This homolog is depicted herein as SEQ ID NO:9. It is believed that SEQ ID NO:9 or the protein encoded thereby (SEQ ID NO:15) may also be useful as a diagnostic marker for detection of ovarian, lung, uterine and endometrial cancer in human patients.

Sequence CWU 1

1

16 1 2587 DNA Homo sapien 1 ggaaggcagc gggcagctcc actcagccag tacccagata cgctgggaac cttccccagc 60 catggcttcc ctggggcaga tcctcttctg gagcataatt agcatcatca ttattctggc 120 tggagcaatt gcactcatca ttggctttgg tatttcaggg agacactcca tcacagtcac 180 tactgtcgcc tcagctggga acattgggga ggatggaatc ctgagctgca cttttgaacc 240 tgacatcaaa ctttctgata tcgtgataca atggctgaag gaaggtgttt taggcttggt 300 ccatgagttc aaagaaggca aagatgagct gtcggagcag gatgaaatgt tcagaggccg 360 gacagcagtg tttgctgatc aagtgatagt tggcaatgcc tctttgcggc tgaaaaacgt 420 gcaactcaca gatgctggca cctacaaatg ttatatcatc acttctaaag gcaaggggaa 480 tgctaacctt gagtataaaa ctggagcctt cagcatgccg gaagtgaatg tggactataa 540 tgccagctca gagaccttgc ggtgtgaggc tccccgatgg ttcccccagc ccacagtggt 600 ctgggcatcc caagttgacc agggagccaa cttctcggaa gtctccaata ccagctttga 660 gctgaactct gagaatgtga ccatgaaggt tgtgtctgtg ctctacaatg ttacgatcaa 720 caacacatac tcctgtatga ttgaaaatga cattgccaaa gcaacagggg atatcaaagt 780 gacagaatcg gagatcaaaa ggcggagtca cctacagctg ctaaactcaa aggcttctct 840 gtgtgtctct tctttctttg ccatcagctg ggcacttctg cctctcagcc cttacctgat 900 gctaaaataa tgtgccttgg ccacaaaaaa gcatgcaaag tcattgttac aacagggatc 960 tacagaacta tttcaccacc agatatgacc tagttttata tttctgggag gaaatgaatt 1020 catatctaga agtctggagt gagcaaacaa gagcaagaaa caaaaagaag ccaaaagcag 1080 aaggctccaa tatgaacaag ataaatctat cttcaaagac atattagaag ttgggaaaat 1140 aattcatgtg aactagacaa gtgtgttaag agtgataagt aaaatgcacg tggagacaag 1200 tgcatcccca gatctcaggg acctccccct gcctgtcacc tggggagtga gaggacagga 1260 tagtgcatgt tctttgtctc tgaattttta gttatatgtg ctgtaatgtt gctctgagga 1320 agcccctgga aagtctatcc caacatatcc acatcttata ttccacaaat taagctgtag 1380 tatgtaccct aagacgctgc taattgactg ccacttcgca actcaggggc ggctgcattt 1440 tagtaatggg tcaaatgatt cactttttat gatgcttcca aaggtgcctt ggcttctctt 1500 cccaactgac aaatgccaaa gttgagaaaa atgatcataa ttttagcata aacagagcag 1560 tcggcgacac cgattttata aataaactga gcaccttctt tttaaacaaa caaatgcggg 1620 tttatttctc agatgatgtt catccgtgaa tggtccaggg aaggaccttt caccttgact 1680 atatggcatt atgtcatcac aagctctgag gcttctcctt tccatcctgc gtggacagct 1740 aagacctcag ttttcaatag catctagagc agtgggactc agctggggtg atttcgcccc 1800 ccatctccgg gggaatgtct gaagacaatt ttggttacct caatgaggga gtggaggagg 1860 atacagtgct actaccaact agtggataaa ggccagggat gctgctcaac ctcctaccat 1920 gtacaggacg tctccccatt acaactaccc aatccgaagt gtcaactgtg tcaggactaa 1980 gaaaccctgg ttttgagtag aaaagggcct ggaaagaggg gagccaacaa atctgtctgc 2040 ttctcacatt agtcattggc aaataagcat tctgtctctt tggctgctgc ctcagcacag 2100 agagccagaa ctctatcggg caccaggata acatctctca gtgaacagag ttgacaaggc 2160 ctatgggaaa tgcctgatgg gattatcttc agcttgttga gcttctaagt ttctttccct 2220 tcattctacc ctgcaagcca agttctgtaa gagaaatgcc tgagttctag ctcaggtttt 2280 cttactctga atttagatct ccagaccctt cctggccaca attcaaatta aggcaacaaa 2340 catatacctt ccatgaagca cacacagact tttgaaagca aggacaatga ctgcttgaat 2400 tgaggccttg aggaatgaag ctttgaagga aaagaatact ttgtttccag cccccttccc 2460 acactcttca tgtgttaacc actgccttcc tggaccttgg agccacggtg actgtattac 2520 atgttgttat agaaaactga ttttagagtt ctgatcgttc aagagaatga ttaaatatac 2580 atttcct 2587 2 2070 DNA Homo sapien 2 cacagagaga ggcagcagct tgctcagcgg acaaggatgc tgggcgtgag ggaccaaggc 60 ctgccctgca ctcgggcctc ctccagccag tgctgaccag ggacttctga cctgctggcc 120 agccaggacc tgtgtgggga ggccctcctg ctgccttggg gtgacaatct cagctccagg 180 ctacagggag accgggagga tcacagagcc agcatgttac aggatcctga cagtgatcaa 240 cctctgaaca gcctcgatgt caaacccctg cgcaaacccc gtatccccat ggagaccttc 300 agaaaggtgg ggatccccat catcatagca ctactgagcc tggcgagtat catcattgtg 360 gttgtcctca tcaaggtgat tctggataaa tactacttcc tctgcgggca gcctctccac 420 ttcatcccga ggaagcagct gtgtgacgga gagctggact gtcccttggg ggaggacgag 480 gagcactgtg tcaagagctt ccccgaaggg cctgcagtgg cagtccgcct ctccaaggac 540 cgatccacac tgcaggtgct ggactcggcc acagggaact ggttctctgc ctgtttcgac 600 aacttcacag aagctctcgc tgagacagcc tgtaggcaga tgggctacag cagcaaaccc 660 actttcagag ctgtggagat tggcccagac caggatctgg atgttgttga aatcacagaa 720 aacagccagg agcttcgcat gcggaactca agtgggccct gtctctcagg ctccctggtc 780 tccctgcact gtcttgcctg tgggaagagc ctgaagaccc cccgtgtggt gggtggggag 840 gaggcctctg tggattcttg gccttggcag gtcagcatcc agtacgacaa acagcacgtc 900 tgtggaggga gcatcctgga cccccactgg gtcctcacgg gcagcccact gcttcaggaa 960 acataccgat gtgttcaact ggaaggtgcg ggcaggctca gacaaactgg gcagcttccc 1020 atccctggct gtggccaaga tcatcatcat tgaattcaac cccatgtacc ccaaagacaa 1080 tgacatcgcc ctcatgaagc tgcagttccc actcactttc tcaggcacag tcaggcccat 1140 ctgtctgccc ttctttgatg aggagctcac tccagccacc ccactctgga tcattggatg 1200 gggctttacg aagcagaatg gagggaagat gtctgacata ctgctgcagg cgtcagtcca 1260 ggtcattgac agcacacggt gcaatgcaga cgatgcgtac cagggggaag tcaccgagaa 1320 gatgatgtgt gcaggcatcc cggaaggggg tgtggacacc tgccagggtg acagtggtgg 1380 gcccctgatg taccaatctg accagtggca tgtggtgggc atcgttagct ggggctatgg 1440 ctgcgggggc ccgagcaccc caggagtata caccaaggtc tcagcctatc tcaactggat 1500 ctacaatgtc tggaaggctg agctgtaatg ctgctgcccc tttgcagtgc tgggagccgc 1560 ttccttcctg ccctgcccac ctggggatcc cccaaagtca gacacagagc aagagtcccc 1620 ttgggtacac ccctctgccc acagcctcag catttcttgg agcagcaaag ggcctcaatt 1680 cctataagag accctcgcag cccagaggcg cccagaggaa gtcagcagcc ctagctcggc 1740 cacacttggt gctcccagca tcccagggag agacacagcc cactgaacaa ggtctcaggg 1800 gtattgctaa gccaagaagg aactttccca cactactgaa tggaagcagg ctgtcttgta 1860 aaagcccaga tcactgtggg ctggagagga gaaggaaagg gtctgcgcca gccctgtccg 1920 tcttcaccca tccccaagcc tactagagca agaaaccagt tgtaatataa aatgcactgc 1980 cctactgttg gtatgactac cgttacctac tgttgcattg ttattacagc tatggccact 2040 attattaaag agctgtgtaa catctctggc 2070 3 1709 DNA Homo sapien 3 agcagactca caccagaact acattccctg gccccctgcc tgtgtgcttc tggccaggcc 60 ttggttggca agtctgaccc gagaaaagga tctgcagaaa atcagactat gggatcactt 120 tgtttgtgca ttgggaatga cattctttcc caccccagga aaacctttgg gactttcaga 180 gacattgtgg ctagccaacc acatggtcag cctcaaagtt gagaggctca gtaaccctcc 240 tatccctaga gaattccaaa gtgtggatgt aatttaacta gaaagccatt ggtgactatc 300 tgtgatcctc tggaagtatg ctatgttgtg tatatcttgc atccaaagcc agagggaacc 360 acaatgacta gtaaaacggt ggtctcaatg cccacttagc ctctgcctct gaatttgacc 420 atagtggcgt tcagctgata gagcgggaag aagaaatatg cattttttat gaaaaaataa 480 atatccaaga gaagatgaaa ctaaatggag aaattgaaat acatctactg gaagaaaaga 540 tccaattcct gaaaatgaag attgctgaga agcaaagaca aatttgtgtg acccagaaat 600 tactgccagc caagaggtcc ctggatgccg acctagctgt gctccaaatt cagttttcac 660 agtgtacaga cagaattaaa gacctggaga aacagttcgt aaagcctgat ggtgagaata 720 gagctcgctt ccttccaggg aaagatctga ccgaaaaaga aatgatccaa aaattagaca 780 agctggaact acaactggcc aagaaggagg agaagctgct ggagaaggat ttcatctatg 840 agcaggtctc caggctcaca gacaggctct gcagcaaaac tcagggctgc aagcaggaca 900 cactgctctt agccaagaag atgaatggct atcaaagaag gatcaaaaat gcaactgaga 960 aaatgatggc tcttgttgct gagctgtcca tgaaacaagc cctaaccatt gaactccaaa 1020 aggaagtcag ggagaaagaa gacttcatct tcacttgcaa ttccaggata gaaaaaggtc 1080 tgccactcaa taaggaaatt gagaaagaat ggttgaaagt ccttcgagat gaagaaatgc 1140 acgccttggc catcgctgaa aagtctcagg agttcttgga agcagataat cgccagctgc 1200 ccaatggtgt ttacacaact gcagagcagc gtccgaatgc ctacatccca gaagcagatg 1260 ccactcttcc tttgccaaaa ccttatggtg ctttggctcc ttttaaaccc agtgaacctg 1320 gagccaatat gaggcacata aggaaacctg ttataaagcc agttgaaatc tgaatatgtg 1380 aacaaatcca ggcctctcaa ggaaaagact tcaaccaggc ttccttgtac ccacaggtga 1440 aaaatgtgag cataatactt ctaatattat tgataagtaa ggtaaccaca attagtcagc 1500 aacagagtac aacagggttt ctatttaccc accaactact atacctttca tgacgttgaa 1560 tgggacatag aactgtccta catttatgtc aaagtatata tttgaatcgc ttatattttc 1620 tttttcactc tttatattga gtacattcca gaaatttgta gtaggcaagg tgctataaaa 1680 atgcactaaa aataaatctg ttctcaatg 1709 4 257 DNA Homo sapien 4 ttaatgggta agtatttttt atatgcttta gctatagcta aagaaaactg atacttaaca 60 aagttgaata gtattattca ctggtgctcc taaaatattg tttttcagtg taaaatatgc 120 atatcttcta tatttaatat gaaagtcttg aaatgtatca gacagaaggg gatttcagtt 180 tgcaaataat gagcaatgta gcaattttaa cacatttcat aaatatatat tttgtcattg 240 gtggagagca ccatttg 257 5 359 DNA Homo sapien 5 gcctgagagc acttagcgtt catgagtgtc cccaccatgg cctggatgat gcttctcctc 60 ggactccttg cttatggatc aggtcaggga gtggattctc agactgtggt gacccaagag 120 ccatcgttat cagtgtcccc tggagggaca gtcacactca cttgtggctt ggcctctgac 180 tcagtctcta ctaatttctt ccccacctgg taccagcaga ccccaggcca ggctccacgc 240 acgctcatct acagcacaag cactcgctct tctggggtcc ctgatcgttt ctctggctcc 300 atccttggga acaaagctgc cctcaccatt acgggggccc aggcagatga tgaatctga 359 6 1372 DNA Homo sapien misc_feature (6)..(6) n = a, c, g or t 6 ccttanagnc ttggttgcca aacagaatgc ccatatccgt cttacttgtg aggaagcttg 60 ccttgggcgc cctctgctgg ccctcctgaa gctaacaggg gcgagtgctc ggtggtttac 120 aaattgcctc catgcagact atgaaactgt tcagcctgct atagttagat ctctggcact 180 ggcccaggag gtcttgcaga tttgcagatc aaggagaacc caggagtttc aaagaagcgg 240 ctagtaaagg tctctgagat ccttgcacta gctacatcct cagggtagga ggaagatggc 300 ttccagaagc atgcggctgc tcctattgct gagctgcctg gccaaaacag gagtcctggg 360 tgatatcatc atgagaccca gctgtgctcc tgggatggtt ttaccacaag tccaattgct 420 atggttactt caggaagctg aggaactggt ctgatgccga gctcgagtgt cagtcttacg 480 gaaacggagc ccacctggca tctatcctga gtttaaagga agccagcacc atagcagagt 540 acataagtgg ctatcagaga agccagccga tatggattgg cctgcacgac ccacagaaga 600 ggcagcagtg gcagtggatt gatggggcca tgtatctgta cagatcctgg tctggcaagt 660 ccatgggtgg gaacaagcac tgtgctgaga tgagctccaa taacaacttt ttaacttgga 720 gcagcaacga atgcaacaag cgccaacact tcctgtgcaa gtaccgacca tagagcaaga 780 atcaagattc tgctaactcc tgcacagccc cgtcctcttc ctttctgcta gcctggctaa 840 atctgctcat tatttcagag gggaaaccta gcaaactaag agtgataagg gccctactac 900 actggctttt ttaggcttag agacagaaac tttagcattg gcccagtagt ggcttctagc 960 tctaaatgtt tgccccgcca tccctttcca cagtatcctt cttccctcct cccctgtctc 1020 tggctgtctc gagcagtcta gaagagtgca tctccagcct atgaaacagc tgggtctttg 1080 gccataagaa gtaaagattt gaagacagaa ggaagaaact caggagtaag cttctagccc 1140 ccttcagctt ctacaccctt ctgccctctc tccattgcct gcaccccacc ccagccactc 1200 aactcctgct tgtttttcct ttggccatgg gaaggtttac cagtagaatc cttgctaggt 1260 tgatgtgggc catacattcc tttaataaac cattgtgtac ataagaggtt gctgtgttcc 1320 agttcagtaa atggtgaatg tggaaaagtg aaataagacc aagaaataca aa 1372 7 291 DNA Homo sapien misc_feature (277)..(277) n= a, c, g, or t 7 agaatggtag tagtaagaag aagaaaaata gaggatctga atgtattttg aaggtagagt 60 ccactggact tagagatgga ttgaatgtgg aagattaagg aaagggagaa atgaaagata 120 gtcttaggtt tcatcttcag atgactgggt gaacagcagt gttctttgct aagatgggga 180 agactaggga aaagagccag ttctgtattg agcatattat atttaagaca atcccatctg 240 ggtccaaaga caatgttgat tttttttctt agatacntgc cctttagacc t 291 8 1275 DNA Homo sapien misc_feature (410)..(410) n= a, c, g, or t 8 attctagaac atatgtataa gctaaaaaca gtattttact cagatcagta gttatcgtgt 60 ctatcagcta taaaaaaaat caactgccag ccaagaactt taaaacttta agctgtgtat 120 tatagaaccg ttttgtgtag cattggaata ttgtccattt tgtaagtcat tgtgaatgtt 180 cttaattatc agcttgaagg tatttttgta ttaaaagttg acattgaaga acctaagtgg 240 atgatgggat ttggggccag tagtgaaagt atgtttcctc taaaatattt ccctaaacag 300 tggtatacat ggttatttta ttatgagatt tgtatatgtt ctgtgtttct ctgtgaacaa 360 tgtttcagtc tctctgtcac catatgtaag gggaagtcca caaatatagn actacattgc 420 acaaaactaa aattgttaat tacaagaaaa tataggtgct taccttttga aggtttatta 480 atacatatgg ttgtcacaat acgtatatat gataaatggt gtacatatac agatgtttat 540 ggtgtataaa tttttctata cccaattaga attatcttcc tgattcttta ttcaataaca 600 tgctaattcc tcttctatgt tctatagtga cagaatgcta acttttctta taccctggca 660 gaggacagag gagtctggtc taggatgggg aactgaattt ttgaacgaaa aggaaagaga 720 aaggatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaat gtttcttagt cattttgatt 780 ggccatttga acagtctaca agtttaacgt tatttccagt gaagtaggat ggctgaccta 840 gcaatacatg tttcttcaaa agggtaaaca tgctttagtg acctaaagct aaattttgta 900 catttgacat caggggtgtt ataagtactg cacttaatac aaagctattt ctcaatngtg 960 ttatttttga gacaaatttt tcttcaccat taacttcttg ttggtagctt tttgttttgt 1020 aaaaattgag agatggcaat gcttatctca accagattat ccatctgcag aattaaggta 1080 tgcaactggt aaataaaaga caaatgctcc agtttgtctt tctcaacctt tgagttctta 1140 acctttgagt taaaacctag tctaaatagt gggaatgtct tggtttacag taaggttttc 1200 ttgggaagga tcttggtttt gtgatctatt tgtgaattaa ggagtagatg ttaaccatta 1260 ttttatagat aagtg 1275 9 2479 DNA Homo sapien 9 gtcatattga acattccaga tacctatcat tactcgatgc tgttgataac agcaagatgg 60 ctttgaactc agggtcacca ccagctattg gaccttacta tgaaaaccat ggataccaac 120 cggaaaaccc ctatcccgca cagcccactg tggtccccac tgtctacgag gtgcatccgg 180 ctcagtacta cccgtccccc gtgccccagt acgccccgag ggtcctgacg caggcttcca 240 accccgtcgt ctgcacgcag cccaaatccc catccgggac agtgtgcacc tcaaagacta 300 agaaagcact gtgcatcacc ttgaccctgg ggaccttcct cgtgggagct gcgctggccg 360 ctggcctact ctggaagttc atgggcagca agtgctccaa ctctgggata gagtgcgact 420 cctcaggtac ctgcatcaac ccctctaact ggtgtgatgg cgtgtcacac tgccccggcg 480 gggaggacga gaatcggtgt gttcgcctct acggaccaaa cttcatcctt cagatgtact 540 catctcagag gaagtcctgg caccctgtgt gccaagacga ctggaacgag aactacgggc 600 gggcggcctg cagggacatg ggctataaga ataattttta ctctagccaa ggaatagtgg 660 atgacagcgg atccaccagc tttatgaaac tgaacacaag tgccggcaat gtcgatatct 720 ataaaaaact gtaccacagt gatgcctgtt cttcaaaagc agtggtttct ttacgctgtt 780 tagcctgcgg ggtcaacttg aactcaagcc gccagagcag gatcgtgggc ggtgagagcg 840 cgctcccggg ggcctggccc tggcaggtca gcctgcacgt ccagaacgtc cacgtgtgcg 900 gaggctccat catcaccccc gagtggatcg tgacagccgc ccactgcgtg gaaaaacctc 960 ttaacaatcc atggcattgg acggcatttg cggggatttt gagacaatct ttcatgttct 1020 atggagccgg ataccaagta caaaaagtga tttctcatcc aaattatgac tccaagacca 1080 agaacaatga cattgcgctg atgaagctgc agaagcctct gactttcaac gacctagtga 1140 aaccagtgtg tctgcccaac ccaggcatga tgctgcagcc agaacagctc tgctggattt 1200 ccgggtgggg ggccaccgag gagaaaggga agacctcaga agtgctgaac gctgccaagg 1260 tgcttctcat tgagacacag agatgcaaca gcagatatgt ctatgacaac ctgatcacac 1320 cagccatgat ctgtgccggc ttcctgcagg ggaacgtcga ttcttgccag ggtgacagtg 1380 gagggcctct ggtcacttcg aacaacaata tctggtggct gataggggat acaagctggg 1440 gttctggctg tgccaaagct tacagaccag gagtgtacgg gaatgtgatg gtattcacgg 1500 actggattta tcgacaaatg aaggcaaacg gctaatccac atggtcttcg tccttgacgt 1560 cgttttacaa gaaaacaatg gggctggttt tgcttccccg tgcatgattt actcttagag 1620 atgattcaga ggtcacttca tttttattaa acagtgaact tgtctggctt tggcactctc 1680 tgccatactg tgcaggctgc agtggctccc ctgcccagcc tgctctccct aaccccttgt 1740 ccgcaagggg tgatggccgg ctggttgtgg gcactggcgg tcaattgtgg aaggaagagg 1800 gttggaggct gcccccattg agatcttcct gctgagtcct ttccaggggc caattttgga 1860 tgagcatgga gctgtcactt ctcagctgct ggatgacttg agatgaaaaa ggagagacat 1920 ggaaagggag acagccaggt ggcacctgca gcggctgccc tctggggcca cttggtagtg 1980 tccccagcct acttcacaag gggattttgc tgatgggttc ttagagcctt agcagccctg 2040 gatggtggcc agaaataaag ggaccagccc ttcatgggtg gtgacgtggt agtcacttgt 2100 aaggggaaca gaaacatttt tgttcttatg gggtgagaat atagacagtg cccttggtgc 2160 gagggaagca attgaaaagg aacttgccct gagcactcct ggtgcaggtc tccacctgca 2220 cattgggtgg ggctcctggg agggagactc agccttcctc ctcatcctcc ctgaccctgc 2280 tcctagcacc ctggagagtg aatgcccctt ggtccctggc agggcgccaa gtttggcacc 2340 atgtcggcct cttcaggcct gatagtcatt ggaaattgag gtccatgggg gaaatcaagg 2400 atgctcagtt taaggtacac tgtttccatg ttatgtttct acacattgat ggtggtgacc 2460 ctgagttcaa agccatctt 2479 10 576 DNA Homo sapien 10 ttcaaagaca tattagaagt tgggaaaata attcatgtga actagacaag tgtgttaaga 60 gtgataagta aaatgcacgt ggagacaagt gcatccccag atctcaggga cctccccctg 120 cctgtcacct ggggagtgag aggacaggat agtgcatgtt ctttgtctct gaatttttag 180 ttatatgtgc tgtaatgttg ctctgaggaa gcccctggaa agtctatccc aacatatcca 240 catcttatat tccacaaatt aagctgtagt atgtacccta agacgctgct aattgactgc 300 cacttcgcaa ctcaggggcg gctgcatttt agtaatgggt caaatgattc actttttatg 360 atgcttccaa aggtgccttg gcttctcttc ccaactgaca aatgccaaag ttgagaaaaa 420 tgatcataat tttagcataa acagagcagt cggcgacacc gattttataa ataaactgag 480 caccttcttt ttaaacaaac aaatgcgggt ttatttctca gatgatgttc atccgtgaat 540 ggtccaggga aggacctttc accttgacta tatggc 576 11 890 DNA Homo sapien 11 caagctctga ggcttctcct ttccatcctg cgtggacagc taagacctca gttttcaata 60 gcatctagag cagtgggact cagctggggt gatttcgccc cccatctccg ggggaatgtc 120 tgaagacaat tttggttacc tcaatgaggg agtggaggag gatacagtgc tactaccaac 180 tagtggataa aggccaggga tgctgctcaa cctcctacca tgtacaggga cgtctcccca 240 ttacaactac ccaatccgaa gtgtcaactg tgtcaggact aagaaaccct ggttttgagt 300 agaaaagggc ctggaaagag gggagccaac aaatctgtct gcttcctcac attagtcatt 360 ggcaaataag cattctgtct ctttggctgc tgcctcagca cagagagcca gaactctatc 420 gggcaccagg ataacatctc tcagtgaaca gagttgacaa ggcctatggg aaatgcctga 480 tgggattatc ttcagcttgt tgagcttcta agtttctttc ccttcattct accctgcaag 540 ccaagttctg taagagaaat gcctgagttc tagctcaggt tttcttactc tgaatttaga 600 tctccagacc cttcctggcc acaattcaaa ttaaggcaac aaacatatac cttccatgaa 660 gcacacacag acttttgaaa gcaaggacaa tgactgcttg aattgaggcc ttgaggaatg 720 aagctttgaa ggaaaagaat actttgtttc cagccccctt cccacactct tcatgtgtta 780 accactgcct tcctggacct tggagccacg gtgactgtat tacatgttgt tatagaaaac 840 tgattttaga gttctgatcg ttcaagagaa tgattaaata tacatttcct 890 12 406 DNA Homo sapien misc_feature (30)..(30) n= a, c, g, or t 12 gtgaatgtgg actataatgc cagctcagan accttgcggt gtgaggctcc ccgatggttc 60 ccccagccca cagtggtctg ggcatcccaa gttgaccagg gagccaactt ctcggaagtc 120 tccaatacca gctttgagct gaactctgag aatgtgacca tgaaggttgt gtctgtgctc 180 tacaatgtta cgatcaacaa cacatactcc tgtatgattg aaaatgacat tgccaaagca 240 acaggggnta tcaaagtgac agaatcggag atcaaaaggc ggagtcacct acagctgcta 300 aactcaaagg cttctctgtg tgtctcttct ttctttgcca tcagctgggc acttctgcct 360 ctcagccctt acctgatgct aanataatgt gccttggcca caaaaa 406 13 462 DNA Homo sapien 13 ggaaggcagc ggcagctcca

ctcagccagt acccagatac gctgggaacc ttccccagcc 60 atggcttccc tggggcagat cctcttctgg agcataatta gcatcatcat tattctggct 120 ggagcaattg cactcatcat tggctttggt atttcaggga gacactccat cacagtcact 180 actgtcgcct cagctgggaa cattggggag gatggaatcc tgagctgcac ttttgaacct 240 gacatcaaac tttctgatat cgtgatacaa tggctgaagg aaggtgtttt aggcttggtc 300 catgagttca aagaaggcaa agatgagctg tcggagcagg atgaaatgtt cagaggccgg 360 acagcagtgt ttgctgatca agtgatagtt ggcaatgcct ctttgcggct gaaaaacgtg 420 caactcacag atgctggcac ctacaaatgt tatatcatca ct 462 14 272 DNA Homo sapien 14 gcagcttgct cagcggacaa ggatgctggg cgtgagggac caaggcctgc cctgcactcg 60 ggcctcctcc agccagtgct gaccagggac ttctgacctg ctggccagcc aggacctgtg 120 tggggaggcc ctcctgctgc cttggggtga caatctcagc tccaggctac agggagaccg 180 ggaggatcac agagccagca tggatcctga cagtgatcaa cctctgaaca gcctcgtcaa 240 ggtgattctg gataaatact acttcctctg cg 272 15 492 PRT Homo sapien 15 Met Ala Leu Asn Ser Gly Ser Pro Pro Ala Ile Gly Pro Tyr Tyr Glu 1 5 10 15 Asn His Gly Tyr Gln Pro Glu Asn Pro Tyr Pro Ala Gln Pro Thr Val 20 25 30 Val Pro Thr Val Tyr Glu Val His Pro Ala Gln Tyr Tyr Pro Ser Pro 35 40 45 Val Pro Gln Tyr Ala Pro Arg Val Leu Thr Gln Ala Ser Asn Pro Val 50 55 60 Val Cys Thr Gln Pro Lys Ser Pro Ser Gly Thr Val Cys Thr Ser Lys 65 70 75 80 Thr Lys Lys Ala Leu Cys Ile Thr Leu Thr Leu Gly Thr Phe Leu Val 85 90 95 Gly Ala Ala Leu Ala Ala Gly Leu Leu Trp Lys Phe Met Gly Ser Lys 100 105 110 Cys Ser Asn Ser Gly Ile Glu Cys Asp Ser Ser Gly Thr Cys Ile Asn 115 120 125 Pro Ser Asn Trp Cys Asp Gly Val Ser His Cys Pro Gly Gly Glu Asp 130 135 140 Glu Asn Arg Cys Val Arg Leu Tyr Gly Pro Asn Phe Ile Leu Gln Met 145 150 155 160 Tyr Ser Ser Gln Arg Lys Ser Trp His Pro Val Cys Gln Asp Asp Trp 165 170 175 Asn Glu Asn Tyr Gly Arg Ala Ala Cys Arg Asp Met Gly Tyr Lys Asn 180 185 190 Asn Phe Tyr Ser Ser Gln Gly Ile Val Asp Asp Ser Gly Ser Thr Ser 195 200 205 Phe Met Lys Leu Asn Thr Ser Ala Gly Asn Val Asp Ile Tyr Lys Lys 210 215 220 Leu Tyr His Ser Asp Ala Cys Ser Ser Lys Ala Val Val Ser Leu Arg 225 230 235 240 Cys Leu Ala Cys Gly Val Asn Leu Asn Ser Ser Arg Gln Ser Arg Ile 245 250 255 Val Gly Gly Glu Ser Ala Leu Pro Gly Ala Trp Pro Trp Gln Val Ser 260 265 270 Leu His Val Gln Asn Val His Val Cys Gly Gly Ser Ile Ile Thr Pro 275 280 285 Glu Trp Ile Val Thr Ala Ala His Cys Val Glu Lys Pro Leu Asn Asn 290 295 300 Pro Trp His Trp Thr Ala Phe Ala Gly Ile Leu Arg Gln Ser Phe Met 305 310 315 320 Phe Tyr Gly Ala Gly Tyr Gln Val Gln Lys Val Ile Ser His Pro Asn 325 330 335 Tyr Asp Ser Lys Thr Lys Asn Asn Asp Ile Ala Leu Met Lys Leu Gln 340 345 350 Lys Pro Leu Thr Phe Asn Asp Leu Val Lys Pro Val Cys Leu Pro Asn 355 360 365 Pro Gly Met Met Leu Gln Pro Glu Gln Leu Cys Trp Ile Ser Gly Trp 370 375 380 Gly Ala Thr Glu Glu Lys Gly Lys Thr Ser Glu Val Leu Asn Ala Ala 385 390 395 400 Lys Val Leu Leu Ile Glu Thr Gln Arg Cys Asn Ser Arg Tyr Val Tyr 405 410 415 Asp Asn Leu Ile Thr Pro Ala Met Ile Cys Ala Gly Phe Leu Gln Gly 420 425 430 Asn Val Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Leu Val Thr Ser 435 440 445 Asn Asn Asn Ile Trp Trp Leu Ile Gly Asp Thr Ser Trp Gly Ser Gly 450 455 460 Cys Ala Lys Ala Tyr Arg Pro Gly Val Tyr Gly Asn Val Met Val Phe 465 470 475 480 Thr Asp Trp Ile Tyr Arg Gln Met Lys Ala Asn Gly 485 490 16 890 DNA Homo sapien misc_feature (168)..(237) n=a, c, g or t 16 caagctctga ggcttctcct ttccatcctg cgtggacagc taagacctca gttttcaata 60 gcatctagag cagtgggact cagctggggt gatttcgccc cccatctccg ggggaatgtc 120 tgaagacaat tttggttacc tcaatgaggg agtggaggag gatacagnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat 240 tacaactacc caatccgaag tgtcaactgt gtcaggacta agaaaccctg gttttgagta 300 gaaaagggcc tgggaaagag gggagccaac aaatctgtct gcttcctcac attagtcatt 360 ggcaaataag cattctgtct ctttggctgc tgcctcagca cagagagcca gaactctatc 420 gggcaccagg ataacatctc tcagtgaaca gagttgacaa ggcctatggg aaatgcctga 480 tgggattatc ttcagcttgt tgagcttcta agtttctttc ccttcattct accctgcaag 540 ccaagttctg taagagaaat gcctgagttc tagctcaggt tttcttactc tgaatttaga 600 tctccagacc ctgcctggcc acaattcaaa ttaaggcaac aaacatatac cttccatgaa 660 gcacacacag acttttgaaa gcaaggacaa tgactgcttg aattgaggcc ttgaggaatg 720 aagctttgaa ggaaaagaat actttgtttc cagccccctt cccacactct tcatgtgtta 780 accactgcct tcctggacct tggagccacg gtgactgtat tacatgttgt tatagaaaac 840 tgattttaga gttctgatcg ttcaagagaa tgattaaata tacatttcct 890

Claims

1. A method for detecting the presence of a selected cancer in a patient comprising: (a) measuring levels of CSG in cells, tissues or bodily fluids in a patient; and (b) comparing the measured levels of CSG with levels of CSG in cells, tissues or bodily fluids from a normal human control, wherein a change in measured levels of CSG in said patient versus normal human control is associated with the presence of a selected cancer.

2-5. (canceled)

6. The method of claim 1 wherein the CSG comprises SEQ ID NO:1, 10, 11, 12 or 13 and the selected cancer is a gynecologic cancer selected from the group consisting of breast, ovarian, endometrial and uterine cancer.

7. The method of claim 1 wherein the CSG comprises SEQ ID NO:2, 9 or 14 and the selected cancer is lung cancer or a gynecologic cancer selected from the group consisting of ovarian, endometrial and uterine cancer.

8. The method of claim 1 wherein the CSG comprises SEQ ID NO:1, 2, 3, 9, 10, 11, 12, 13 or 14 and the selected cancer is ovarian cancer.

9. An antibody against a CSG wherein said CSG comprises SEQ ID NO:1, 2, 3, 9, 10, 11, 12, 13 or 14.

10. A method of imaging or treating a selected cancer in a patient comprising administering to the patient an antibody of claim 9.

11. The method of claim 10 wherein said antibody is labeled with paramagnetic ions or a radioisotope.

12. (canceled)

13. The method of claim 10 wherein the antibody is conjugated to a cytotoxic agent.