U.S. patent application number 10/961368 was filed with the patent office on 2005-07-07 for amidomethyl-substituted 2-(4-sulfonylamino)-3-hydroxy-3,4-dihydro-2h-chrom- en-6-yl compounds, a process and intermediates for their production, and pharmaceutical compositions containing them.
This patent application is currently assigned to Solvay Pharmaceuticals GmbH. Invention is credited to Boecker, Christiane, Brueckner, Reinhard, Fischer, Yvan, Marrison, Lester, Mlinaric, Michael, Moloney, Brian, Sykes, David, Weske, Michael, Witte, Klaus, Ziegler, Dieter.
Application Number | 20050148659 10/961368 |
Document ID | / |
Family ID | 34713985 |
Filed Date | 2005-07-07 |
United States Patent
Application |
20050148659 |
Kind Code |
A1 |
Sykes, David ; et
al. |
July 7, 2005 |
Amidomethyl-substituted
2-(4-sulfonylamino)-3-hydroxy-3,4-dihydro-2H-chrom- en-6-yl
compounds, a process and intermediates for their production, and
pharmaceutical compositions containing them
Abstract
Compounds corresponding to formula I: 1 in which R.sup.1,
R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 have the meanings
given in the description; a process for the preparation of these
compounds; intermediate products used to prepare such compounds,
and pharmaceutical compositions containing such compounds.
Inventors: |
Sykes, David; (Didcot,
GB) ; Moloney, Brian; (Didcot, GB) ; Marrison,
Lester; (Didcot, GB) ; Ziegler, Dieter;
(Hemmingen, DE) ; Mlinaric, Michael; (Hannover,
DE) ; Boecker, Christiane; (Hannover, DE) ;
Brueckner, Reinhard; (Hannover, DE) ; Weske,
Michael; (Burgdorf, DE) ; Witte, Klaus;
(US) ; Fischer, Yvan; (US) |
Correspondence
Address: |
CROWELL & MORING LLP
INTELLECTUAL PROPERTY GROUP
P.O. BOX 14300
WASHINGTON
DC
20044-4300
US
|
Assignee: |
Solvay Pharmaceuticals GmbH
Hannover
DE
|
Family ID: |
34713985 |
Appl. No.: |
10/961368 |
Filed: |
October 12, 2004 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
60536505 |
Jan 15, 2004 |
|
|
|
Current U.S.
Class: |
514/456 ;
549/403 |
Current CPC
Class: |
C07D 407/12 20130101;
C07D 311/68 20130101 |
Class at
Publication: |
514/456 ;
549/403 |
International
Class: |
A61K 031/353; C07D
045/02; C07D 311/76 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 17, 2003 |
DE |
103 48 298.9 |
Claims
What is claimed is:
1. A compound corresponding to formula I: 17wherein R.sup.1 is
C.sub.1-4-alkyl, R.sup.2 is C.sub.1-4-alkyl, R.sup.3 is phenyl
which is optionally substituted 1 or 2 times by halogen,
C.sub.1-4-alkyl, C.sub.1-4-alkoxy or trifluoromethyl; naphthyl or
biphenyl, R.sup.4 is hydrogen; C.sub.1-6-alkyl or
C.sub.3-7-cycloalkyl-C.sub.1-4-alkyl, R.sup.5 is hydrogen, and
R.sup.6 is C.sub.1-6-alkyl; phenyl-C.sub.1-4-alkyl, the phenyl
group of which is optionally substituted once by halogen;
furyl-C.sub.1-4-alkyl or tetrahydronaphthyl, or R.sup.5 and
R.sup.6, together with the nitrogen to which they are bonded, form
a piperazine ring which may optionally be substituted by
phenyl.
2. A compound according to claim 1, wherein R.sup.1 and R.sup.2 are
each methyl.
3. A compound according to claim 1, wherein R.sup.3 is phenyl or
mono-substituted phenyl.
4. A compound according to claim 1, wherein R.sup.4 is hydrogen,
C.sub.1-6alkyl or cyclopropyl-C.sub.1-4-alkyl.
5. A compound according to claim 1, wherein R.sup.6 is
phenyl-C.sub.1-4-alkyl or tetrahydronaphthyl.
6. A compound according to claim 1, wherein in the pyran ring the
C-3 carbon bearing the hydroxy substituent is in the S
configuration and the C-4 carbon bearing the nitrogen-containing
substituent is in the R configuration.
7. A compound according to claim 1, selected from the group
consisting of:
2-(4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4-dihydro--
2H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphthalen-1-yl acetamide;
2-((3S,4R)-4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4--
dihydro-2H-chromen-6-yl)-N-[(1R)-1,2,3,4-tetrahydronaphthalen-1-yl]acetami-
de;
N-benzyl-2-{4-[[(4-ethylphenyl)sulfonyl](neopentyl)amino]-3-hydroxy-2,-
2-dimethyl-3,4-dihydro-2H-chromen-6-yl}acetamide;
2-{4-[[(4-ethylphenyl)su-
lfonyl](neopentyl)amino]-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-y-
l}-N-(2-phenylethyl)acetamide, and P0
2-(4-([(4-methylphenyl)sulfonyl]amin-
o)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-1,2,3,4-tetrahydr-
onaphthalen-1-yl acetamide.
8. A pharmaceutical composition comprising a compound according to
claim 1, and at least one pharmaceutical auxiliary or carrier.
9. A method of treating a disease state in a patient suffering from
said disease state, said method comprising administering a
pharmaceutically effective amount of at least one compound
according to claim 1 to said patient, wherein said disease state is
selected from the group consisting of cardiovascular diseases,
proliferative diseases, chronic inflammatory diseases and
autoimmune diseases.
10. A process for preparing a compound corresponding to Formula I:
18wherein R.sup.1 is C.sub.1-4-alkyl, R.sup.2 is C.sub.1-4-alkyl,
R.sup.3 is phenyl which is optionally substituted 1 to 2 times by
halogen, C.sub.1-4-alkyl, C.sub.1-4-alkoxy or trifluoromethyl;
naphthyl or biphenyl, R.sup.4 is hydrogen; C.sub.1-6-alkyl or
C.sub.3-7cycloalkyl-C.s- ub.1-4-alkyl, R.sup.5 is hydrogen, and
R.sup.6 is C.sub.1-6-alkyl; phenyl-C.sub.1-4-alkyl, the phenyl
group of which is optionally substituted once by halogen;
furyl-C.sub.1-4-alkyl or tetrahydronaphthyl, or R.sup.5 and
R.sup.6, together with the nitrogen to which they are bonded, form
a piperazine ring which may optionally be substituted by phenyl,
said method comprising reacting a compound corresponding to formula
II: 19wherein R.sup.1, R.sup.2, R.sup.4, R.sup.5 and R.sup.6 have
the above meanings, with a compound corresponding to formula III:
X--SO.sub.2--R.sup.3 III wherein R.sup.3 has the above meaning, and
X is a cleavable leaving group.
11. A compound corresponding to formula II: 20wherein R.sup.1 is
C.sub.1-4-alkyl, R.sup.2 is C.sub.1-4-alkyl, R.sup.4 is hydrogen;
C.sub.1-6-alkyl or C.sub.3-7-cycloalkyl-C.sub.1-4-alkyl, R.sup.5 is
hydrogen, and R.sup.6 is C.sub.1-6-alkyl; phenyl-C.sub.1-4-alkyl,
the phenyl group of which is optionally substituted once by
halogen; furyl-C.sub.1-4-alkyl or tetrahydronaphthyl, or R.sup.5
and R.sup.6, together with the nitrogen to which they are bonded,
form a piperazine ring which may optionally be substituted by
phenyl.
Description
BACKGROUND OF THE INVENTION
[0001] The present invention relates to novel
amidomethyl-substituted
2-(4-sulfonylamino)-3-hydroxy-3,4-dihydro-2H-chromen-6-yl
derivatives with a potassium channel-blocking effect, in particular
with an effect influencing the cardiovascular system, and also to
medicaments containing these compounds. Furthermore, the invention
relates to a process for the preparation of the novel compounds and
intermediate products of this process.
[0002] Indanes, benzopyrans and analogues of such compounds which
have potassium channel-blocking effects, and in particular effects
beneficially influencing the cardiovascular system, are known from
U.S. Pat. No. 6,150,356 (.dbd.WO 00/12077).
[0003] Published international application no. WO 00/58300
discloses chroman derivatives which are suitable as medicaments, in
particular antiarhythmically effective medicaments.
SUMMARY OF THE INVENTION
[0004] It was an object of the present invention to provide new
pharmaceutically active substances useful for treating disease
conditions.
[0005] Another object of the invention was to provide active
substances useful in treating cardiovascular diseases, especially
cardiac arrhythmias.
[0006] A further object of the invention was to provide
pharmaceutically active substances characterized by high
effectiveness and patient tolerability.
[0007] It is also an object of the invention to provide active
substances which exhibit an antiarrhythmic activity with a marked
atrial-selective activity profile. These and other objects have
been achieved in accordance with the present invention by providing
a compound corresponding to formula I: 2
[0008] wherein
[0009] R.sup.1 is C.sub.1-4-alkyl,
[0010] R.sup.2 is C.sub.1-4-alkyl,
[0011] R.sup.3 is phenyl which is optionally substituted 1 or 2
times by halogen, C.sub.1-4-alkyl, C.sub.1-4-alkoxy or
trifluoromethyl; naphthyl or biphenyl,
[0012] R.sup.4 is hydrogen; C.sub.1-6-alkyl or
C.sub.3-7cycloalkyl-C.sub.1- -4-alkyl,
[0013] R.sup.5 is hydrogen, and
[0014] R.sup.6 is C.sub.1-6-alkyl; phenyl-C.sub.1-4-alkyl, the
phenyl group of which is optionally substituted once by halogen;
furyl-C.sub.1-4-alkyl or tetrahydronaphthyl, or
[0015] R.sup.5 and R.sup.6, together with the nitrogen to which
they are bonded, form a piperazine ring which may optionally be
substituted by phenyl.
[0016] It has now surprisingly been found that a group of novel
amidomethyl-substituted
2-(4-sulfonylamino)-3-hydroxy-3,4-dihydro-2H-chro- men-6-yl
compounds according to the invention possess potassium
channel-blocking properties and are suitable for the treatment of
cardiovascular diseases, preferably for the treatment of cardiac
arrhythmias. The compounds according to the invention are
distinguished by high effectiveness with good compatibility and in
the case of anti-arrhythmic action also by a marked
atrial-selective action profile. In addition, the compounds
according to the invention have properties which lead one to expect
an additional effect influencing the immune system.
[0017] The invention thus relates to novel amidomethyl-substituted
2-(4-sulfonylamino)-3-hydroxy-3,4-dihydro-2H-chromen-6-yl compounds
corresponding to formula I: 3
[0018] wherein
[0019] R.sup.1 is C.sub.1-4-alkyl,
[0020] R.sup.2 is C.sub.1-4-alkyl,
[0021] R.sup.3 is phenyl, which is optionally substituted 1 to 2
times by halogen, C.sub.1-4-alkyl, C.sub.1-4-alkoxy or
trifluoromethyl; naphthyl or biphenyl,
[0022] R.sup.4 is hydrogen; C.sub.1-6-alkyl or
C.sub.3-7-cycloalkyl-C.sub.- 1-4-alkyl,
[0023] R.sup.5 is hydrogen, and
[0024] R.sup.5 is C.sub.1-6-alkyl; phenyl-C.sub.1-4-alkyl, the
phenyl group of which is optionally substituted once by halogen;
furyl-C.sub.1-4-alkyl or tetrahydronaphthyl, or
[0025] R.sup.5 and R.sup.6, together with the nitrogen to which
they are bonded, form a piperazine ring which may optionally be
substituted by phenyl.
[0026] Furthermore, the invention relates to pharmaceutical
compositions containing the compounds of Formula I. Furthermore,
the invention relates to a process for the preparation of the
compounds of Formula I and to intermediate products of this
process.
[0027] Whenever the compounds of Formula I or other compounds
described within the context of the present invention include
substituents consisting of or comprising C.sub.1-4-alkyl or
C.sub.1-6-alkyl groups, these groups may each be straight-chain or
branched.
[0028] R.sup.1 and R.sup.2 preferably each have the meaning
methyl.
[0029] R.sup.3 preferably has the meaning phenyl which is
optionally substituted 1 to 2 times by halogen, C.sub.1-4-alkyl,
C.sub.1-4-alkoxy or trifluoromethyl. In particular, R.sup.3 has the
meaning of phenyl substituted once by C.sub.1-4-alkyl. Where
R.sup.3 is halogen-substituted phenyl, fluorine, chlorine, bromine
and iodine are considered as halogen. As a particularly preferred
meaning, R.sup.3 stands for 4-ethylphenyl.
[0030] R.sup.4 is preferably hydrogen, C.sub.1-6-alkyl or
cyclopropyl-C.sub.1-4-alkyl, in particular cyclopropylmethyl. Where
R.sup.4 stands for C.sub.1-6-alkyl, this is in particular branched
and preferably represents neopentyl, 2,2-dimethylbutyl,
2-ethylbutyl, 3-methylbutyl or 2-methylpropyl.
[0031] R.sup.5 is preferably hydrogen.
[0032] R.sup.6 preferably has the meanings phenyl-C.sub.1-4-alkyl,
in particular benzyl or phenethyl, or the meaning
tetrahydronaphthyl, in particular 1-tetrahydronaphthyl.
(R)-1-tetrahydronaphthyl is preferred.
[0033] Particularly preferred compounds of Formula I are selected
from the group consisting of:
[0034]
2-(4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4-di-
hydro-2H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl
acetamide;
[0035]
2-((3S,4R)-4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethy-
l-3,4-dihydro-2H-chromen-6-yl)-N-[(1R)-1,2,3,4-tetrahydronaphth-1-yl]aceta-
mide;
[0036]
N-benzyl-2-{4-[[(4-ethylphenyl)sulfonyl](neopentyl)amino]-3-hydroxy-
-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl}acetamide;
[0037]
2-{4-[[(4-ethylphenyl)sulfonyl](neopentyl)amino]-3-hydroxy-2,2-dime-
thyl-3,4-dihydro-2H-chromen-6-yl}-N-(2-phenylethyl)acetamide,
and
[0038]
2-(4-{[(4-methylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4-d-
ihydro-2H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl
acetamide.
[0039] According to the invention, the novel compounds of Formula I
are obtained by reacting a compound corresponding to formula II:
4
[0040] wherein R.sup.1, R.sup.2, R.sup.4, R.sup.5, and R.sup.6 have
the above meanings,
[0041] with a compound corresponding to formula III:
X--SO.sub.2--R.sup.3 III
[0042] wherein R.sup.3 has the above meaning and X is a cleavable
leaving group. The reaction can be carried out using a conventional
wet-chemical process in an organic solvent which is inert under the
reaction conditions, in particular a dipolar-aprotic solvent such
as dichloromethane or in a mixture of such solvents and in the
presence of a base. Suitable bases include non-nucleophilic organic
nitrogen bases such as tertiary lower alkylamines, for example
triethylamine. Liquid organic bases used in excess can also be used
as solvents. If desired, the reaction can be catalyzed by a known
coupling aid such as 4-N,N-dimethylaminopyridine (=DMAP). Suitable
reaction temperatures are between room temperature and 80.degree.
C., for example 65.degree. C. Suitable reaction pressures are
between normal pressure and approximately 200 bar, for example 180
bar. If the compound of Formula III which is used is liquid, it may
be advantageous to remove the solvent used from the reaction
mixture after the addition of the compound of Formula III to the
compound of Formula II dissolved in the solvent in known manner,
for example at reduced pressure. Whenever in the starting compounds
of Formula II R.sup.4 stands for hydrogen, it is advantageous to
use equimolar amounts of compound of Formula III. Usually halogen,
preferably chlorine or bromine, is used as leaving group X in
compounds of Formula III.
[0043] Furthermore, the reaction of a compound of Formula II with a
compound of Formula III can also be performed in known manner on a
solid phase, in particular on a reactive resin such as aminomethyl
polystyrene (AMPS). This reaction variant can preferably be used
for the preparation of smaller amounts of substance, for example on
a scale of 1 to 10 mmol. Where synthesis is on a solid phase,
preferably a readily filterable base such as known
polymer-supported methylpiperidine (.dbd.PS methylpiperidine) can
be used as base. Suitable reaction temperatures for solid-phase
synthesis are between 10.degree. C. and 40.degree. C., preferably
room temperature. Compounds of Formula I may be isolated in known
manner from the reaction mixture and if necessary purified in known
manner.
[0044] Compounds of Formula II can be prepared by reacting an
epoxide compound corresponding to formula IV: 5
[0045] wherein R.sup.1, R.sup.2, R.sup.5 and R.sup.6 have the above
meanings, in known manner with a nucleophilic organic nitrogen
compound, preferably ammonia in aqueous solution, in a
dipolar-protic solvent such as a lower-alkyl alcohol, preferably
ethanol. Suitable reaction temperatures are between room
temperature and 60.degree. C.
[0046] Where compounds of Formula II are desired in which R.sup.4
represents C.sub.1-6-alkyl or C.sub.3-7-cycloalkyl-C.sub.1-4-alkyl,
the resulting compound of Formula II, in which R.sup.4 represents
hydrogen, can then be alkylated in known manner. The alkylation can
be carried out in particular as an aminoalkylation, by first
reacting the compound of Formula II, in which R.sup.4 stands for
hydrogen, with an aldehyde corresponding to formula V:
R.sup.401--CHO V
[0047] wherein R.sup.401 is hydrogen, C.sub.2-5-alkyl or
C.sub.3-7-cycloalkyl-C.sub.0-3-alkyl, and then reducing the
resulting !mine intermediate product by addition of a reducing
agent to the alkylamine compound of Formula II. Examples of
suitable reducing agents include complex borohydrides such as
NaBH.sub.3CN or known polymer-supported borohydride
(.dbd.PS--BH.sub.4).
[0048] In a first variant, the reaction can be carried out in a
polar-protic organic solvent which is inert under the reaction
conditions, in particular methanol, the reduction of the imine
being performed in situ without isolating it in the same solvent.
Suitable reaction temperatures for this variant are between room
temperature and 60.degree. C., for example 50.degree. C. In a
second variant, the reaction of the compound of Formula II, wherein
R.sup.4 stands for hydrogen, with an aldehyde of Formula V to form
the imine intermediate product can be carried out in a
dipolar-aprotic solvent, in particular tetrahydrofuran (=THF). In
that case, it is advantageous to add catalytic amounts of a
hydrophilic agent, for example an orthoester, in particular
trimethyl orthoformate (=TMOF), to speed up the reaction. Then the
imine intermediate product can be isolated and taken up in a
polar-protic solvent stated above for the first variant, in order
to perform the reduction in this solvent. This second variant may
preferably be carried out at room temperature. In the nucleophilic
ring-opening reaction of epoxides of Formula IV described above in
two variants, as a rule compounds of Formula II are obtained in
which the vicinal substituents in position 3 and in position 4 of
the pyran ring, namely the hydroxyl group and the amino group, are
each in the trans position to one another.
[0049] The compounds of Formula II are themselves novel compounds
which are advantageously suitable as intermediate products for the
preparation of novel pharmacologically active substances, for
example for the preparation of the compounds of Formula I.
[0050] Compounds of Formula III and compounds of Formula V are
known per se or can be prepared in known manner from known
compounds.
[0051] Compounds of Formula IV can be prepared by reacting a
compound corresponding to formula VI: 6
[0052] wherein R.sup.1, R.sup.2, R.sup.5 and R.sup.6 have the above
meanings, in known manner with a peroxide compound capable of
epoxide formation, preferably with m-chloroperbenzoic acid (MCPBA),
in an organic polar-aprotic solvent which is inert under the
reaction conditions, preferably dichloromethane, and in the
presence of a base. An example of a particularly suitable base is
an aqueous solution of sodium hydrogen carbonate. The reaction may
preferably be carried out at room temperature.
[0053] Compounds of Formula I have at least in the vicinal carbon
atoms in position 3 and in position 4 of the pyran ring in each
case an asymmetric center or chiral center and can therefore occur
in several isomeric forms. The invention includes both the
isomerically pure compounds of Formula I and mixtures of these
isomers. The optically active compounds of Formula I can be
obtained, for example, from the mixtures of the isomers of Formula
I or from mixtures of the isomers of Formula II in known manner,
e.g. by chromatographic separation on chiral separating materials.
Mixtures of the isomers of Formula II can also be obtained by
reaction with suitable optically active acids, e.g. camphorsulfonic
acid or D- or L-tartaric acid, and subsequent fractionation into
the respective optical antipodes by fractional crystallization of
the resulting salts.
[0054] The optically active compounds of Formula I can also be
prepared directly by chiral synthesis. Where compounds of Formula I
are to be prepared wherein the hydroxy substituent in position 3 of
the pyran ring and the R.sup.4NSO.sub.2R.sup.3-substituent in
position 4 of the pyran ring are in a stereochemically defined
trans position to one another, in each case the starting point may
be epoxides of Formula IV in which the appropriate stereochemistry
is already predetermined. Epoxides of Formula IV with
correspondingly predetermined stereochemistry can for example be
prepared by epoxidizing alkenes of Formula VI in known manner with
the aid of a chiral catalyst in accordance with the method of
Jacobsen, U.S. Pat. No. 5,637,739 (=EP 521,099). Where for example
a compound of Formula I is to be prepared in which the chiral
center in position 3 of the pyran ring is in the S configuration
and in which the chiral center in position 4 of the pyran ring is
in the R configuration, an intermediate product of Formula VI can
be reacted in the presence of a chiral catalyst, in particular
(S,S)-manganese (III) salen and in the presence of an oxygen donor,
in particular sodium hypochlorite in aqueous solution, in an
organic solvent which is inert under the reaction conditions, in
particular dichloromethane. The reaction may advantageously be
carried out at a pH value between 9.5 and 11.5. To set a suitable
pH value, preferably a buffer consisting of Na.sub.2HPO.sub.4 and
pyridine-N-oxide can be added to the reaction mixture. Suitable
reaction temperatures are between -10.degree. C. and room
temperature, preferably at 0.degree. C. Where a compound of Formula
I is to be prepared in which the chiral center in position 3 of the
pyran ring is in the R configuration and in which the chiral center
in position 4 of the pyran ring is in the S configuration, the
procedure can be analogous to the directions described above, but
(R,R)-manganese (III) salen is then used instead of (S,S)-manganese
(III) salen.
[0055] Compounds of Formula VI can be prepared by reacting a
compound corresponding to formula VIII: 7
[0056] wherein R.sup.1 and R.sup.2 have the above meanings,
[0057] with a compound corresponding to formula VIII:
HNR.sup.5R.sup.6 VIII
[0058] wherein R.sup.5 and R.sup.8 have the above meanings, in a
manner known for aminoacylation. The carboxylic acids of Formula
VII or their reactive derivatives such as acid halides, in
particular acid chlorides or acid bromides, may be used as
acylation agents. If the acids of Formula VII themselves are used
as acylation agents, the reaction thereof with the amino compounds
of Formula VIII can advantageously also be carried out in the
presence of a known coupling reagent, for example
1,1-carbonyldiimidazole, ethyl chloroformate or an alkyl
carbodiimide, e.g. N'-(3-dimethylaminopropyl)-N-ethylcarbodiimide
(=EDC), or a cycloalkyl carbodiimide such as
dicyclohexylcarbodiimide. The acylation may take place in an
organic solvent which is inert under the reaction conditions at
temperatures from -30.degree. C. to +50.degree. C., preferably at
room temperature. Suitable solvents include halogenated
hydrocarbons such as dichloromethane and cyclic ethers such as
tetrahydrofuran or dioxane or mixtures of these solvents.
[0059] Compounds of Formula VII can be prepared by hydrolyzing the
ester group of a compound corresponding to formula IX: 8
[0060] wherein R.sup.1 and R.sup.2 have the above meanings, and
R.sup.7 is C.sub.1-4-alkyl, in known manner. The hydrolysis can be
carried out, for example, in a polar-protic solvent such as
ethylene glycol by contacting with a base, for example a strong
base such as dilute aqueous sodium hydroxide solution. Suitable
reaction temperatures are between room temperature and the boiling
point of the solvent or of the solvent mixture.
[0061] Compounds of Formula IX can be prepared by reacting a
compound corresponding to formula X: 9
[0062] wherein R.sup.7 has the above meaning, with a compound
corresponding to formula XI: 10
[0063] wherein R.sup.1 and R.sup.2 have the above meanings, in
known manner. The reaction can be carried out in an organic solvent
which is inert under the reaction conditions, such as toluene or
xylene and in the presence of an acid with water being separated by
azeotropic distillation. Examples of suitable acids include acetic
acid or propionic acid. Advantageously, the reaction is carried out
with the addition of a catalyst such as a Lewis acid, for example
phenylboronic acid. Suitable reaction temperatures are between room
temperature and the boiling point of the solvent or of the solvent
mixture, for example around 120.degree. C.
[0064] The compounds of Formula X and Formula XI are known per se
or can be prepared in known manner from known compounds.
[0065] The advantageous effects of compounds of Formula I as
pharmacologically active substances will become apparent from the
following background. It is already known that substances which
block endogenous cardiac potassium channels can be used as active
substances to counter cardiovascular diseases, in particular to
counter cardiac arrhythmias. By blocking outward-directed potassium
currents in the heart, a prolongation of the action potential of
the heart can be brought about which has a beneficial effect on
antiarrhythmic heart conditions. Examples of this known treatment
are Class III antiarrhythmic drugs.
[0066] One problem of such non-specific potassium channel blockers
is their low degree of selectivity with respect to their effect on
different heart tissues. Thus for a relatively long time it has
been assumed that in particular Class III antiarrhythmic drugs can
lead to undesirable prolongation of the QT interval in the
electrocardiogram (=ECG) and to polymorphic ventricular
tachycardias ("torsades de pointes"), which can trigger ultimately
undesirable complications such as for example ventricular
fibrillation. For this reason, potassium channel blockers have been
sought which are capable of selectively influencing the potassium
currents of the atrium, but not of the ventricle. Since the
K.sub.v1.5-potassium channels in the heart which were discovered
some time ago are located exclusively in the atrium, but not in the
ventricle, it can be assumed that these K.sub.v1.5-potassium
channel-blocking compounds are suitable as atrial-selective
antiarrhythmic drugs. K.sub.v1.5-potassium channels and other
potassium channels are however located not only in the heart, but
e.g. also in vessels of the body. Therefore it cannot always be
ruled out that K.sub.v1.5-potassium channel-blocking compounds may
lead to increases in blood pressure resulting from the blockade of
potassium channels in the vessels. K.sub.v1.5-potassium
channel-blocking compounds which are free of side-effects which
raise blood pressure are therefore preferred. Further undesirable
side-effects which may occur on administration of many
K.sub.v1.5-potassium channel-blocking compounds are additional
Class I-antiarrhythmic side-effects and also negatively inotropic
effects.
[0067] The compounds of Formula I are distinguished by an effect
which particularly pronouncedly and selectively blocks the cardiac
K.sub.v1.5-potassium channels. In addition to particularly good
effectiveness and a marked atrial-selective antiarrhythmic activity
profile, the compounds of Formula I at most have slight undesirable
side-effects such as increase in blood pressure, Class
I-antiarrhythmic side-effects and negatively inotropic effects. The
compounds of Formula I are therefore indicated for the treatment
and/or prophylaxis of cardiovascular diseases, in particular atrial
fibrillation, atrial flutter and other cardiac arrhythmias, in
larger mammals and humans.
[0068] Furthermore, the compounds of Formula I exhibit a clear
effect of blocking the K.sub.v1.3-potassium channels.
K.sub.v1.3-potassium channels are preferentially located in cells
of the immune system. A connection is made between blockade of the
K.sub.v1.3-potassium channels and inter alia an anti-proliferative
and/or immunosuppressive effect (cf. C. Beeton et al., The Journal
of Immunology 166 (2001) 936-944). It can therefore be assumed of
compounds which are capable of blocking K.sub.v1.3-potassium
channels--for example the compounds of Formula I--that they are
also suitable for the treatment and/or prophylaxis of
proliferative, chronic inflammatory and autoimmune diseases such as
multiple sclerosis.
DESCRIPTION OF PHARMACOLOGICAL TEST METHODS
[0069] The cited example numbers relate to the subsequent
preparation examples.
[0070] 1. In-Vitro Investigation of the K.sub.V1.5-Potassium
Channel-Blocking Effect of the Substances
[0071] The K.sub.v1.5-potassium channel-blocking effect of the
substances was demonstrated in a known test model or analogously to
this test model (cf. W. Hu et al., J. Pharmacol. Toxicol. Methods
34 (1995) 1-7). In this test model, a cell line of egg cells of the
Chinese hamster (="Chinese hamster oocytes", "CHO") is used which
originates from a single cell and stably expresses the
K.sub.v1.5-channel. By incubation overnight in a nutrient medium
containing RbCl or a "loading buffer" (all values in mM: RbCl 5,
NaCl 140, CaCl.sub.2 2, MgSO.sub.4 1, HEPES buffer 10, glucose 5)
the aforementioned oocytes are loaded with Rb.sup.+ under the
influence of Na.sup.+/K.sup.+-ATPase. Thereafter, a portion of the
oocytes is incubated as a reference standard in the absence of an
inhibitor, while another portion of the oocytes is incubated in the
presence of the respective inhibitory test substance of Formula I.
Then the oocytes are depolarized by increasing the extracellular
potassium-ion concentration, which causes the K.sub.v1.5-potassium
channels of the oocytes to open. In the absence of an inhibitor,
the Rb.sup.+ ions flow through the K.sub.v1.5-potassium channels
into the liquid surrounding them. In the presence of an inhibitory
test substance of Formula I, on the other hand, the Rb.sup.+ ions
remain locked within the oocytes. The extent of the
K.sub.v1.5-potassium channel-blocking effect of the test substances
of Formula I is determined by measuring the Rb.sup.+ ion
concentration in the liquid surrounding them by means of atomic
absorption spectroscopy against a reference standard.
[0072] Chinese hamster oocytes (see above) were cultivated in a
known, RbCl-containing nutrient medium for CHO-cells and placed in
the sample wells of a 96-sample capacity sample plate ("96 well
plate"). The oocytes were allowed to grow overnight in order to
obtain monolayers of the oocytes. Then the nutrient medium was
pipetted off, and each sample well was washed three times with 100
.mu.l portions of a preincubation buffer of low potassium-ion
concentration (all values in mM: KCl 5, NaCl 140, CaCl.sub.2 2,
MgSO.sub.4 1, HEPES buffer 10, glucose 5). Then 50 .mu.l of a
solution of the respective test substance (stock solution in DMSO,
dilution with preincubation buffer, final concentration in the test
batch 10 .mu.M) or of the solvent (as negative controls) was added
to each sample well and incubated for 10 min. in each case at room
temperature. Then 50 .mu.l of a stimulation buffer with elevated
potassium-ion concentration (KCl 145 mM, NaCl 0 mM, otherwise as
preincubation buffer) was added to each sample well and the samples
were then incubated for a further 10 min. at room temperature. In
each case, 80 .mu.l of the liquid surrounding the oocytes from each
sample well was then transferred separately to the sample wells of
an analysis sample plate, and the Rb.sup.+ ion concentration in the
liquids was determined by atomic absorption spectroscopy. The test
substances were each double-tested. The signal section which
represented the K.sub.v1.5 component of the Rb.sup.+ outflow was
defined by using as positive control the known potassium channel
blocker 4-AP in a high concentration (100.times.IC.sub.50 for the
K.sub.v1.5 channel). This made it possible to determine which
portion of the Rb.sup.+ outflow was dependent on the influence of
the 4-AP and therefore is to be assigned to the K.sub.v1.5 channel.
For the substances which in the concentration of 10 .mu.M used led
to a reduction in the Rb.sup.+ outflow of at least 50%, additional
tests were performed with lower concentrations of the test
substances in order to be able to determine the half-maximum
effective concentration. In each case the concentration of
half-maximum inhibition of the test substances of Formula I
(IC.sub.50) was given as characteristic variable.
[0073] In this test model the test substances of Formula I listed
in the following Table 1 had the IC.sub.50 values given in the
table.
1TABLE 1 K.sub.v1.5-potassium channel-blocking effect of the test
substances in vitro Example No. IC.sub.50 2 2.0 4 1.6 6 5.0 7 1.5 8
0.5 9 2.9 10 1.6 11 3.2 12 3.2 13 6.5 15 4.0 16 6.3 17 2.65 18 2.7
19 2.5 20 2.9 21 3.3 22 3.4 23 4.3 24 5 25 5.2 26 5.2 27 5.6
[0074] 2. In-Vitro Investigation of the K.sub.v1.3-Potassium
Channel-Blocking Effect of the substances
[0075] The K.sub.v1.3-potassium channel-blocking effect of the
substances was demonstrated in a known test model (e.g. from
Genion, Hamburg) or analogously to this test model (cf. J. Plsek
and Sigler, J. Photochem. Photobiol. 33 (1996) 101-124). In this
test model, known oocytes of the Chinese hamster (.dbd.CHO) are
used which are stably transfected with the K.sub.v1.3-potassium
channel. The blockade of the cell-inherent K.sub.v1.3-potassium
channel activity in the transfected cells is accompanied by a
positive shift in the membrane potential from approx. -40 mV to -30
mV, whereas in the wild-type CHO cells investigated in parallel no
significant shift in the membrane potential is triggered. A change
in the membrane potential is thus connected to the reduction in the
K.sub.v1.3-potassium channel activity. By blocking the
K.sub.v1.3-potassium channels e.g. with substances of Formula I and
the resulting change in the membrane potential, an accumulation of
a membrane potential-sensitive fluorescent dye in intracellular
compartments of the oocytes and ultimately increasing fluorescence
occurs. The change in the membrane potential of the oocytes is
therefore measured indirectly via the increase in fluorescence of
the membrane potential-senstive dyes.
[0076] The cells were transfected with the K.sub.v1.3 plasmid in
known manner with a commercially obtainable transfection reagent
(DMRIE-C from Gibco BRL, Germany). The successful transfection was
verified by immunofluorescence and by "patch-clamp" investigations
of the potassium ion current. The fluorescence measurements were
performed on a Tecan Safire fluorescence reader from Tecan,
Germany. In each case, the increase in the fluorescent intensity
caused by the blockade of the K.sub.v1.3-potassium channels in the
oocytes with substances of Formula I in a concentration of 10 .mu.M
was determined as characteristic variable. The increase in the
fluorescent intensity was given in each case in percent (%)
compared with an increase in the fluorescent intensity caused by
the reference substance margatoxin. Margatoxin is known as a
selective K.sub.v1.3-potassium channel blocker (see e.g. M.
Farcia-Calvo et al., J. Biol. Chem. 268 (1993) 18866-18874).
[0077] In this test model the test substances of Formula I listed
in the following Table 2 had the percentages given in the
table:
2TABLE 2 K.sub.v1.3-potassium channel-blocking effect of the test
substances in vitro Increase in the fluorescent intensity Example
No. (% margatoxin) 2 72 5 97 6 82 9 107 10 82 11 99 12 111 13 53 17
72 18 141 19 171 20 97 21 86 22 71 23 41
[0078] 3. Investigation of the Functional Effectiveness of the
Substances on the Atrium of Rats' Hearts In Vitro
[0079] The functional antiarrhythmic effectiveness of the
substances was demonstrated in the test model set forth below. In
this test model it is determined to what extent the
K.sub.v1.5-blocking substances of Formula I result in a
prolongation of the functional refractory period in the left atrium
of rats. The refractory period is the minimum possible elapsed time
between the basic stimulus and additional stimulus in which a
renewed contraction can be triggered. The extent of the
prolongation of the functional refractory period is a measurement
of the antiarrhythmic effectiveness of the substances according to
the invention. The functional refractory period is determined by
testing on the electrically stimulated preparation at what elapsed
time from the preceding contraction a renewed contraction can be
triggered by additional electrical stimuli.
[0080] The hearts were removed from freshly sacrificed rats
(Sprague-Dawley, Charles-River, Germany). The left atria were
isolated and fastened to force transducers in a
temperature-controlled (30.degree. C.), gasified (O.sub.2 95%,
CO.sub.2 5%) organ bath which was filled with modified Tyrode
solution (all values in mM: NaCl 137; KCl 2.7; CaCl.sub.2 1.8;
MgCl.sub.2 0.8; NaHCO.sub.3 11.9; NaH.sub.2PO.sub.4 0.6; glucose
5). In order to trigger regular contractions, the preparations were
electrically stimulated (rectangular pulses, pulse magnitude
3.5.times. threshold stimulus, pulse width 1.5 ms, frequency 1 Hz).
Initially, the initial value of the functional refractory period
was determined by applying extra pulses in addition to the basic
stimulus, the elapsed time from the preceding basic stimulus being
shortened until no further additional contraction could be
triggered. Then the cumulative addition of increasing
concentrations (0.1-10 .mu.M) of the substances of Formula I took
place at intervals of 20 min. each, the refractory period being
determined again in each case 18 min. after the addition had taken
place. Before the measurement, stock solutions of the test
substances (3.2 and 0.32 mM in 100% DMSO) were prepared. In order
to achieve the desired final concentrations of the substances
(0.1-10 .mu.M) in the organ bath (volume 100 ml), corresponding
volumes of these stock solutions were then poured into the organ
bath.
[0081] In each case the prolongation of the functional refractory
period (FRP) in the left atrium of the rats' hearts in milliseconds
observed after the addition of 10 .mu.M of the respective substance
of Formula I to the atrial preparations was given as characteristic
variable.
[0082] In this test model the test substances of Formula I listed
in the following Table 3 exhibited the prolongations of refractory
period given in the table, with higher values representing a
stronger antiarrhythmic effectiveness:
3TABLE 3 FRP-prolonging effect of the test substances (10 .mu.M) on
the left atria of rats' hearts in vitro Example No. FRP
prolongation [ms] 1 15 2 20 4 17 7 24 8 17 9 30 10 20 11 24 12 14
13 28 14 22 15 30 16 20
[0083] 4. Investigation of the Functional Effectiveness of the
Substances on Guinea-Pig Hearts In Vivo
[0084] In the test model shown below, it was shown that the
substances according to the invention at most have slight
undesirable proarrhythmic effects on repolarization in the
ventricle. To this end, the influence of the compounds of Formula I
on the effective refractory period (ERP) and other influencing
variables on guinea-pig hearts in vivo were investigated. In this
test model, non-selective potassium channel blockers not in
accordance with the invention, which also block HERG and/or
K.sub.vLQT1 channels, result in undesirable prolongation of the ERP
and the QT time on an electrocardiogram (=ECG). The QT time is
likewise a measurement of the repolarization in the heart.
Prolongations of both the ERP and the QT time, respectively, which
are due to the substances were independently interpreted as
indications of the risk of the occurrence of undesirable
torsade-de-pointes arrhythmias. Furthermore, the QRS interval also
was determined in each case from the ECG as a measurement of the
ventricular rate of spread of stimulus. Even a prolongation of the
QRS interval caused by a test substance is connected with an
increased risk of undesirable pro-arrhythmic side-effects.
Therefore in this test model the lack of an ERP and QT time
prolongation signifies a low risk, but the occurrence of a relevant
ERP and QT prolongation on the other hand signifies an elevated
risk of undesirable pro-arrhythmic effects. Also the lack of a
prolongation of the QRS interval which is due to the substances due
to the substances of Formula I investigated indicates a low risk of
undesirable pro-arrhythmic side-effects, since lack of QRS
prolongation indicates an undisturbed spread of stimulus in the
ventricle. Conversely, a QRS prolongation, which is typically
triggered by Class I antiarrhythmic drugs indicates slowing of the
conduction rate and may promote the occurrence of ventricular
tachycardias to ventricular fibrillation.
[0085] Male guinea pigs (Dunkin-Hartley from Charles River) were
anaesthetized (ketamine 50 mg/kg, xylazine 10 mg/kg) and each of
them was provided with a venous access via one jugular vein for
administration of compounds of Formula I or a vehicle. A bipolar
stimulation catheter was fed into the right ventricle of the guinea
pigs via the other jugular vein (stimulation frequency 5 Hz). The
arterial blood pressure was measured by a catheter located in the
carotid artery which was connected to a Statham pressure
transducer. The ECG was recorded via needle electrodes. The
measured data were digitized via an analog/digital converter,
recorded on a computer with suitable software (Ponemah Physiology
Platform from Gould, USA) and printed out in parallel on a
multichannel printer. After an equilibration period of 45 min.,
increasing doses of the compounds of Formula I or of the vehicle
were administered intravenously (=i.v.) to the guinea pigs at
12-minute intervals. Before the first administration and in each
case one minute after administration of increasing doses (0.1-max.
30 .mu.mole/kg) of the substances of Formula I, the effective
refractory period was measured. For this, after five normal stimuli
in each case an additional pulse was applied and the elapsed time
thereof from the preceding pulse was increased until a heart action
was triggered. The observed time interval corresponds to the ERP of
the ventricular myocardium.
[0086] In order to detect possible effects of the test substances
on the blood pressure, in the same test model after each
administration of substance the systolic and diastolic blood
pressure was determined and compared with the previous
blood-pressure level. The parameters were recorded automatically 1
and 8 min. after each administration of substance. Table 4 also
shows changes in systolic blood pressure due to the compounds of
Formula I given below (minus effects due to the vehicle). None of
the compounds listed resulted in a significant blood pressure
increase.
[0087] In this test model, the test substances of Formula I listed
in the following Table 4 had the effects given in the table. Only
statistically significant effects were listed, with a t-test with a
significance limit of P<0.05 being used for the statistical
testing. In Table 4, the indication "n.s." (="not statistically
significant") means that the substance of the corresponding example
does not have any statistically significant influence on the
measured variable listed.
4TABLE 4 Effect of the test substances (1 min. after administration
of 10 .mu.mole/kg i.v.) on the ERP, QT and QRS intervals in the
ventricle of guinea pigs and simultaneously measured changes in the
systolic blood pressure in vivo (n.s. = not statistically
significant, negative values indicate shortening or reduction)
syst. blood ERP QT QRS pressure Ex. No. (ms) (ms) (ms) (mm Hg) 1
n.s. n.s. n.s. n.s. 2 n.s. n.s. n.s. n.s. 4 n.s. n.s. n.s. n.s. 7
-8.2 n.s. n.s. -15.3 8 n.s. n.s. n.s. -10.7 10 -8.0 n.s. n.s. -15.9
11 n.s. n.s. n.s. n.s. 12 n.s. n.s. n.s. n.s. 13 n.s. n.s. n.s.
n.s. 16 -7.5 n.s. n.s. -8.6
[0088] 5. Investigation of the Functional Effectiveness of the
Substances on the Hearts of anaesthetised Cats In Vivo
[0089] In the test model shown below, it was shown that the
substances according to the invention at most have a marked
atrial-selective effect on the heart. After administration of the
substances according to the invention, a significant increase in
the atrial fibrillation threshold--i.e. the current intensity at
which atrial fibrillation occurs--was observed. At the same time,
on the other hand, the ventricular fibrillation threshold is
influenced only minimally.
[0090] Living cats were anaesthetised with chloralose/urethane
(501300 mg/kg i.v.) and were ventilated with ambient air. Then,
following thoracotomy, stimulating electrodes were attached to the
right atrium and the ventricle. The atrial and ventricular
fibrillation threshold was determined in known manner by
administration of rectangular pulses of increasing current
intensity until atrial or ventricular fibrillation occurred (for
performance see in detail: Br. J. Pharmac. 17 (1961) 167; Hdb. exp.
Pharmacol. XVI/3 (1975) 131; Pharmacol. Res. 25 Suppl. 2 (1992)
156). The test substances of Formula I were dissolved in propylene
glycol (80%) and administered intravenously in increasing doses
(5-30 .mu.mol/kg). Atrial and ventricular fibrillation thresholds
were then determined at 5-minute intervals in known manner after
the addition of the respective dose. The increase in the respective
fibrillation threshold due to the substances investigated was
expressed in percent of the value before administration of
substance, i.e. a doubling of the fibrillation threshold
corresponds to an increase of 100%.
[0091] In this test model, the test substances of Formula I listed
in the following Table 5 had the effects given in the table.
5TABLE 5 Increase in the atrial (AFT) and ventricular fibrillation
threshold (VFT) in anaesthetized cats in vivo (dose 30 .mu.mol/kg
i.v.) Selectivity factor Ex. No. AFT (%) VFT (%) (AFT:VFT) 1 66 22
3 2 261 15 17 4 226 19 12 9 213 29 7 10 241 19 13
[0092] The particularly good physiological tolerability of the
compounds according to the invention can also be demonstrated in
further pharmacological test models. Thus for example it can be
demonstrated in an in vitro test on cardiac muscle preparations of
guinea pigs that the compounds of Formula I at most have slight
Class I-side-effects. antiarrhythmic side-effects. Furthermore, it
can be demonstrated in an in vitro model on rats'hearts and in
another in vitro model on guinea pigs' hearts that the compounds of
Formula I at most cause slight negatively inotropic effects.
[0093] The compounds of Formula I may be administered in
conventional pharmaceutical preparations. In an individual case,
special dosage forms may be indicated. The doses to be used may
vary individually and will naturally vary depending on the type of
condition to be treated and the substance used. In general,
however, pharmaceutical dosage forms with an active substance
content of 0.2 to 500 mg, in particular 10 to 200 mg, active
substance per individual dose are suitable for administration to
humans and larger mammals. The compounds may be contained according
to the invention, together with conventional pharmaceutical
auxiliaries and/or carriers, in solid or liquid pharmaceutical
preparations. Examples of solid preparations include preparations
which can be administered orally, such as tablets, coated tablets,
capsules, powders or granules, or alternatively suppositories.
These preparations may contain conventional pharmaceutical
inorganic and/or organic carriers, such as talcum, lactose or
starch, in addition to conventional pharmaceutical auxiliaries, for
example lubricants or tablet disintegrating agents. Liquid
preparations such as suspensions or emulsions of the active
substances may contain the usual diluents such as water, oils
and/or suspension agents such as polyethylene glycols and the like.
Other auxiliaries may additionally be added, such as preservative,
taste correctives and the like.
[0094] The active substances may be mixed and formulated with the
pharmaceutical auxiliaries and/or carriers in known manner. For the
preparation of solid medicament forms, the active substances may
for example be mixed with the auxiliaries and/or carriers in
conventional manner and may be wet or dry granulated. The granules
or powder may be poured directly into capsules or be pressed into
tablet cores in conventional manner. These may be coated in known
manner if desired.
[0095] The following examples are intended to illustrate the
invention further, without limiting its scope.
EXAMPLE 1
2-(4-{[(4-Ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4-dihydro-2-
H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl acetamide
[0096] 11
[0097] A) 25 g methyl-4-hydroxyphenylacetate, 14.5 ml
3-methylbut-2-enal and 18.3 g phenylboronic acid were placed
together under nitrogen atmosphere in 1 l dry toluene and heated to
boiling for 7 hours under reflux cooling. Then 60 ml glacial acetic
acid was added to this receiving solution at room temperature (=RT)
and the mixture was again heated to boiling for 7 hours under
reflux cooling. It was allowed to cool to room temperature, the
solvent was largely evaporated at reduced pressure and the
remaining residue was poured into 300 ml of a 1:1 (v/v) mixture of
ethyl acetate (=EA) and water. The pH value was adjusted to 5 by
addition of solid sodium bicarbonate, the organic phase was
separated off and largely evaporated at reduced pressure.
Chromatography of the remaining residue on silica gel (mobile
phase: petroleum ether/EA 10:1 v/v) yielded 16 g
methyl-(2,2-dimethyl-2H-chromen-6-yl)acetate as pale yellow
oil.
[0098] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.15 (s,
3H) 1.27 (t, 3H) 1.43 (s, 3H) 1.60-1.85 (3H) 1.97 (m, 1H) 2.66-2.82
(4H) 3.20-3.29 (3H) 3.54 (dd, 1H) 4.23 (dd, 1H) 5.06 (m, 1H) 5.28
(d, 1H) 5.59 (d, 1H) 6.55 (d, 1H) 6.66 (d, 1H) 6.97 (dd, 1H)
7.03-7.18 (4H) 7.35 (m, 2H) 7.87 (m, 2H); .sup.13C-NMR (101 MHz,
CDCl.sub.3) .delta. [ppm]: 15.1 (q) 18.5 (q) 20.1 (t) 26.6 (q) 28.8
(t) 29.2 (t) 30.2 (t) 42.9 (t) 47.7 (d) 55.1 (d) 74.8 (d) 78.7 (s)
117.9 (d) 121.3 (s) 126.3 (d) 127.2 (s) 127.4 (d, 3 C) 128.2 (d)
128.8 (d) 128.9 (d, 2 C) 129.3 (d) 130.3 (d) 136.5 (s) 137.6 (s)
137.7 (s) 150.2 (s) 152.3(s) 170.3 (s).
[0099] B) 46 g of the methyl-(2,2-dimethyl-2H-chromen-6-yl)acetate
obtained above (total amount from several analogous batches), 150
ml ethylene glycol and 400 ml of a 20%-strength aqueous sodium
hydroxide solution were heated to boiling for 3 hours in 440 ml
tetrahydrofuran (=THF) under reflux cooling. Then the resulting
mixture was allowed to cool to room temperature, the solvent was
largely evaporated at reduced pressure, and 200 ml tert. butylether
and 300 ml water were added to the remaining residue. It was
stirred for 10 min. and then the aqueous phase was acidulated by
addition of a 20%-strength aqueous hydrochloric acid solution to pH
6. The aqueous phase was extracted twice with 300 ml portions of
dichloromethane and the combined organic phases were dried over 20
g sodium sulfate. The solvent was largely evaporated at reduced
pressure, petroleum ether was added to the remaining residue, and a
first resulting crystal fraction was filtered out from the solvent.
The filtrate was concentrated again at reduced pressure, with
another crystal fraction being produced. The combined crystal
fractions were dried and 33.8 g
2,2-(dimethyl-2H-chromen-6-yl)acetic acid was obtained, which was
used without further purification for the subsequent reaction.
[0100] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.41 (s,
6H) 3.52 (s, 2H) 5.59 (d, 1H) 6.27 (d, 1H) 6.72 (d, 1H) 6.88 (d,
1H) 6.99 (dd, 1H); .sup.13C-NMR (101 MHz, CDCl.sub.3) .delta.
[ppm]: 28.1 (q, 2 C) 40.2 (t) 76.3 (s) 116.5 (d) 121.4 (s) 122.1
(d) 125.3 (s) 127.2 (d) 129.9 (d) 131.1 (d) 152.3 (s) 177.8
(s).
[0101] C) 9.6 g 1,1-carbonyldiimidazole (=CDl) dissolved in 85 ml
THF was slowly added to a solution of 11.7 g of the
2,2-dimethyl-2H-chromen-6-yl acetic acid obtained above in 100 ml
THF and stirred for 30 min. at room temperature. 8.8 ml
1,2,3,4-tetrahydro-1-naphthylamine, dissolved in 30 ml THF, was
dropped slowly into this receiving solution, the resulting mixture
was stirred for 1 hour and left to stand overnight at room
temperature. The solvent was largely evaporated at reduced
pressure, and the remaining residue was stirred with a mixture of
diethyl ether and isopropanol (100:1 v/v) and crystallized. The
resulting crystals were removed by suction and dried at 65.degree.
C. and 20 bar. Chromatography of the diethyl ether/isopropanol
washing liquid on silica gel yielded further intermediate product,
which was combined with the main quantity and dried. 17.5 g
2-(2,2-dimethyl-2H-chromen-6-yl)-N-1,2,3,4-tetrahydrona- phth-1-yl
acetamide was obtained as colorless solid, which was used without
further purification for the subsequent reaction.
[0102] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.41 (s,
6H) 1.60-1.85 (3H) 1.98-2.08 (1H) 2.65-2.80 (2H) 3.45-3.55 (2H)
5.12-5.20 (1H) 5.60 (d, 1H) 5.66 (d, 1H) 6.26 (d, 1H) 6.71 (d, 1H)
6.86 (d, 1H) 6.96 (dd, 1H) 7.02-7.07 (1H) 7.08-7.17 (3H);
.sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 20.2 (t) 28.0 (q,
2 C) 29.2 (t) 30.3 (t) 43.2 (t) 47.7 (d) 76.3 (s) 116.8 (d) 121.7
(s) 122.0 (d) 126.2 (d) 126.9 (s) 127.2 (d) 128.2 (d) 129.1 (d)
129.8 (d) 131.3 (d) 136.7 (s) 137.5 (s) 152.2 (s) 170.7 (s).
[0103] D) 950 ml of a saturated aqueous sodium hydrogen carbonate
solution was added to a solution of 11 g of the
2-(2,2-dimethyl-2H-chromen-6-yl)-N- -1,2,3,4-tetrahydronaphth-1-yl
acetamide obtained above in 600 ml dichloromethane. A total of 15 g
m-chloroperoxybenzoic acid (=MCPBA) in three 5 g portions was added
to this receiving solution at 5 min. intervals, and the mixture was
stirred for 18 hours at room temperature. The organic phase was
separated and substantially entirely evaporated on a rotary
evaporator at 65.degree. C. and 20 bar. 2-(2,2-dimethyl-1a,7b-di-
hydro-2H-oxireno[c]chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl
acetamide was obtained as crude oil, which was used without further
purification for the subsequent reaction.
[0104] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.23 (s,
6H) 1.56 (s, 6H) 1.63-1.85 (6H) 1.97-2.10 (2H) 2.65-2.82 (4H)
3.43-3.56 (6H) 3.84-3.89 (2H) 5.12-5.22 (2H) 5.62-5.72 (2H) 6.75
(d, 1H) 6.76 (d, 1H) 7.02-7.19 (10H) 7.23-7.27 (2H); .sup.13C-NMR
(101 MHz, CDCl.sub.3) .delta. [ppm]: 20.1 (t) 22.6 (q) 25.7 (q)
29.2 (t) 30.2 (t) 43.1 (t) 47.7 (d) 50.8 (d) 62.7 (d) 73.2 (s)
118.6 (d) 120.5 (s) 126.2 (d) 126.3 (d) 127.2 (d) 127.3 (d) 127.5
(s) 128.2 (d) 128.3 (d) 129.2 (d) 130.5 (d) 131.1 (d) 131.2 (d)
136.5 (s) 137.5 (s) 137.6 (s) 151.8 (s) 170.4 (s).
[0105] E) 88 ml of a 25%-strength aqueous ammonia solution was
added to a solution of 16 g of the
2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chrome-
n-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl acetamide obtained above in
88 ml ethanol, and the mixture was stirred for 18 hours at room
temperature. Then 200 ml dichloromethane and 50 ml methanol were
added thereto, and the mixture was stirred for a further 15
minutes. Then 200 ml water was added and the mixture was again
stirred for 15 minutes. The organic phase was separated and largely
evaporated at reduced pressure. The remaining residue was stirred
with 30 ml of ethyl acetate, filtered and dried on a rotary
evaporator at 70.degree. C. and 20 bar. 3.1 g
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-1,2,3,4--
tetrahydronaphth-1-yl acetamide was obtained as grey solid, which
was used without further purification for the subsequent
reaction.
[0106] .sup.1H-NMR (400 MHz, DMSO-D.sub.6) .delta. [ppm]: 1.08 (s,
6H) 1.35 (s, 6H) 1.60-1.95 (12H) 2.63-2.83 (4H) 3.17 (d, 2H)
3.32-3.40 (4H) 3.49 (d, 2H) 4.90-4.98 (2H) 5.32-5.38 (2H) 6.62 (d,
2H) 7.01 (dd, 2H) 7.05-7.18 (8H) 7.47 (d, 2H) 8.32-8.35 (2H);
.sup.13C-NMR (101 MHz, DMSO-D.sub.6) .delta. [ppm]: 18.6 (q) 19.9
(t) 27.0 (q) 28.7 (t) 29.8 (t) 41.7 (t) 46.2 (d) 50.8 (d) 76.6 (d)
77.8 (s) 115.6 (d) 125.5 (s) 125.6 (d) 125.7 (d) 126.5 (d) 127.9
(s) 128.0 (d) 128.1 (d) 128.3 (d) 128.4 (d) 128.6 (d) 136.9 (s)
137.5 (s) 150.5 (s) 169.8 (s).
[0107] F) 1.67 g 4-ethyl benzenesulfonyl chloride was added
dropwise to a solution of 3.75 g of the
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2-
H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl acetamide obtained
above and 8.4 ml triethylamine in 160 ml dichloromethane. In
addition 5 ml of dimethyl formamide (=DMF) was added, and the
resulting mixture was stirred for 18 hours at room temperature.
Then 100 ml water was added, and the mixture was stirred for
another 5 minutes. The organic phase was separated, and the solvent
was evaporated at reduced pressure. The remaining residue was
chromatographed on silica gel (mobile phase: ethyl
acetate/cydohexane/methanol 110:140:2 v/v/v), and the product
phases were combined and reduced in volume. The residue was stirred
with petroleum ether/diethyl ether 10:1 v/v. The resulting crystals
were removed by suction and dried on a rotary evaporator at
40.degree. C. and 25 bar. 2.3 g of the title compound was obtained
as colorless crystals.
[0108] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: m 1.14 (s,
3H) 1.16 (s, 3H) 1.26 (t, 3H) 1.28 (t, 3H) 1.44 (s, 6H) 1.60-1.85
(8H) 1.95-2.08 (2H) 2.66-2.83 (8H) 3.18-3.33 (6H) 3.53-3.63 (2H)
4.18-4.28 (2H) 5.02-5.13 (2H) 5.26-5.37 (2H) 5.52-5.60 (2H) 6.54
(d, 2H) 6.66-6.71 (2H) 6.95-7.19 (m, 10H) 7.34-7.39 (4H) 7.85-7.92
(4H); .sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 15.1 (q)
18.4 (q) 20.1 (t) 26.6 (q) 28.8 (t) 29.2 (t) 30.2 (t) 42.9 (t) 47.7
(d) 47.8 (d) 55.1 (d) 74.9 (d) 75.0 (d) 78.6 (s) 78.7 (s) 118.0 (d)
121.0 (s) 126.2 (d) 126.3 (d) 127.2 (s) 127.4 (d) 128.2 (d) 128.8
(d) 128.9 (d) 129.2 (d) 129.3 (d) 130.3 (d) 136.5 (s) 137.6 (s)
150.3 (s) 152.3 (s) 170.3 (s).
EXAMPLE 2
2-((3S,4R)-4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-dimethyl-3,4-d-
ihydro-2H-chromen-6-yl)-N-[(1R)-1,2,3,4-tetrahydronaphth-1-yl]acetamide
[0109] 12
[0110] A) 24.5 g CDl, 30 g 2,2-(dimethyl-2H-chromen-6-yl)acetic
acid (for preparation see Example 1B)) and 22.8 ml
(1R)-1,2,3,4-tetrahydro-1-naphth- ylamine were reacted according to
the procedure set forth in Example 1C). 49 g
2-(2,2-dimethyl-2H-chromen-6-yl)-N-[(1R)-1,2,3,4-tetrahydronaphth-1--
yl]acetamide was obtained as colorless crystals, which was used
without further purification for the subsequent reaction.
[0111] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.41 (s,
6H) 1.60-1.85 (3H) 1.98-2.08 (1H) 2.65-2.80 (2H) 3.45-3.55 (2H)
5.12-5.20 (1H) 5.60 (d, 1H) 5.66 (d, 1H) 6.26 (d, 1H) 6.71 (d, 1H)
6.86 (d, 1H) 6.96 (dd, 1H) 7.02-7.07 (1H) 7.08-7.17 (3H);
.sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 20.2 (t) 28.0 (q,
2 C) 29.2 (t) 30.3 (t) 43.2 (t) 47.7 (d) 76.3 (s) 116.8 (d) 121.7
(s) 122.0 (d) 126.2 (d) 126.9 (s) 127.2 (d) 128.2 (d) 129.1 (d)
129.8 (d) 131.3 (d) 136.7 (s) 137.5 (s) 152.2 (s) 170.7 (s).
[0112] B) 5 g (S,S)-manganese-(III)-salen and 7 g pyridine-N-oxide
were added to a solution of 44 g of the
2-(2,2-dimethyl-2H-chromen-6-yl)-N-[(1-
R)-1,2,3,4-tetrahydronaphth-1-yl]acetamide obtained above in 800 ml
dichloromethane. The resulting receiving solution was cooled to
0.degree. C., and a mixture of 660 ml of an aqueous sodium
hypochlorite solution (Cl>13%) and 88 ml of a 9%-strength
aqueous solution of Na.sub.2HPO.sub.4 were added over 45 minutes.
Stirring was continued for a further 3 hours at 0.degree. C., then
the organic phase was separated and stirred for 1 hour with 500 g
Celite.RTM. 503. The solid was filtered out and the filtrate was
washed with dichloromethane until it was colorless. The filtrate
was evaporated to dryness at reduced pressure. 40 g
2-[(1aS,7bS)-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chromen-6-yl]-N-1-
,2,3,4-tetrahydronaphth-1-yl acetamide was obtained as crude oil,
which was used without further purification or characterization for
the subsequent reaction.
[0113] C) 40 g of the
2-[(1aS,7bS)-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[-
c]chromen-6-yl]-N-1,2,3,4-tetrahydronaphth-1-yl acetamide obtained
above and 250 ml of a 25%-strength aqueous ammonia solution were
reacted in a manner corresponding to the procedure of Example 1E).
Chromatography of the crude product on silica gel (mobile phase:
dichloromethane/methanol/2- 5%-strength aqueous ammonia solution
(75:50:2 v/v/v)) yielded 13.2 g
2-[(3S,4R)-4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl]-N--
[(1R)-1,2,3,4-tetrahydronaphth-1-yl]acetamide as oil, which was
used without further purification for the subsequent reaction.
[0114] .sup.1H-NMR (400 MHz, DMSO-D.sub.6) .delta. [ppm]: 1.08 (s,
6H) 1.35 (s, 6H) 1.60-1.95 (12H) 2.63-2.83 (4H) 3.17 (d, 2H)
3.32-3.40 (4H) 3.49 (d, 2H) 4.90-4.98 (2H) 5.32-5.38 (2H) 6.62 (d,
2H) 7.01 (dd, 2H) 7.05-7.18 (8H) 7.47 (d, 2H) 8.32-8.35 (2H);
.sup.13C-NMR (101 MHz, DMSO-D.sub.6) .delta. [ppm]: 18.6 (q) 19.9
(t) 27.0 (q) 28.7 (t) 29.8 (t) 41.7 (t) 46.2 (d) 50.8 (d) 76.6 (d)
77.8 (s) 115.6 (d) 125.5 (s) 125.6 (d) 125.7 (d) 126.5 (d) 127.9
(s) 128.0 (d) 128.1 (d) 128.3 (d) 128.4 (d) 128.6 (d) 136.9 (s)
137.5 (s) 150.5 (s) 169.8 (s).
[0115] D) 5.94 ml 4-ethyl benzenesulfonyl chloride, 13.2 g of the
2-[(3S,4R)-4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl]-N--
[(1R)-1,2,3,4-tetrahydronaphth-1-yl]acetamide obtained above, 88 ml
triethylamine and 4 ml dimethyl formamide were reacted in a manner
corresponding to the procedure of Example 1F). 6.2 g of the title
compound was obtained as solid, amorphous foam.
[0116] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.15 (s,
3H) 1.26 (t, J=7.6 Hz, 3H) 1.45 (s, 3H) 1.60-1.85 (3H) 2.01 (m, 1H)
2.65-2.82 (4H) 3.20-3.33 (3H) 3.58 (dd, J=9.0, 3.0 Hz, 1H) 4.23
(dd, J=9.0, 8.7 Hz, 1H) 5.04 (m, 1H) 5.16 (d, J=8.7 Hz, 1H) 5.51
(d, J=8.5 Hz, 1H) 6.54 (d, J=2.0 Hz, 1H) 6.68 (d, J=8.4 Hz, 1H)
6.98 (dd, J=8.4, 2.0 Hz, 1H) 6.95-7.19 (4H) 7.37 (m, 2H) 7.87 (m,
2H); .sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 15.1 (q)
18.4 (q) 20.1 (t) 26.6 (q) 28.9 (t) 29.2 (t) 30.2 (t) 43.0 (t) 47.8
(d) 55.1 (d) 75.0 (d) 78.7 (s) 118.0 (d) 121.1 (s) 126.2 (d) 127.3
(s) 127.4 (d, 3 C) 128.2 (d) 128.7 (d) 129.0 (d, 2 C) 129.3 (d)
130.4 (d) 136.5 (s) 137.6 (s) 137.7 (s) 150.4 (s) 152.3 (s) 170.3
(s); [.alpha.].sub.D.sup.20=-8.3.degree. (c=0.1, MeOH).
EXAMPLE 3
N-benzyl-2-{4-[[(4-ethylphenyl)sulfonyl](neopentyl)amino]-3-hydroxy-2,2-di-
methyl-3,4-dihydro-2H-chromen-6-yl}acetamide
[0117] 13
[0118] A) 16.2 g of CDl dissolved in 300 ml tetrahydrofuran (THF)
was slowly added to a solution of 19.7 g
2,2-dimethyl-2H-chromen-6-yl)acetic acid (for preparation see
Example 1B)) in 300 ml THF and stirred for 10 minutes at room
temperature. 10.9 ml benzylamine was added slowly dropwise to this
receiving solution, and the resulting mixture was stirred for 1
hour. The solvent was largely evaporated at reduced pressure, and
the remaining residue was extracted once with 500 ml of a mixture
of ethyl acetate and water (2:3 v/v). The organic phase was largely
evaporated at reduced pressure, and the remaining residue was
chromatographed on silica gel (mobile phase: EA/cyclohexane 1:1
v/v). Drying of the product fractions on a rotary evaporator at
70.degree. C. and 20 bar yielded 28 g
N-benzyl-2-(2,2-dimethyl-2H-chromen-6-yl)acetamid- e as oil, which
was used without further purification or characterization for the
subsequent reaction.
[0119] B) 980 ml of a saturated aqueous sodium hydrogen carbonate
solution was added to a solution of 28 g of the
N-benzyl-2-(2,2-dimethyl-2H-chrome- n-6-yl)acetamide obtained above
in 480 ml dichloromethane. A total of 44.1 g MCPBA in three 14.7 g
portions was added to this receiving solution at 5 min. intervals,
and the mixture was stirred for 18 hours at room temperature. The
organic phase was separated, extracted once with 200 ml of a
saturated aqueous sodium hydrogen carbonate solution, and the
organic phase was then substantially completely evaporated at
reduced pressure. 35 g
N-benzyl-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chrome-
n-6-yl)acetamide was obtained as crude oil, which was used without
further purification or characterization for the subsequent
reaction.
[0120] C) 250 ml of a 25%-strength aqueous ammonia solution was
added to a solution of 35 g of the
N-benzyl-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno-
[c]chromen-6-yl)acetamide obtained above in 250 ml ethanol, and the
mixture was stirred for 18 hours at room temperature. The reaction
mixture was poured into 500 ml water and extracted with 250 ml
dichloromethane. The organic phase was separated, dried over sodium
sulfate and largely evaporated under reduced pressure. 200 ml
diethyl ether was added to the remaining residue. The crystals
produced after a while were removed by suction and dried on a
rotary evaporator at 60.degree. C. and 20 bar. 11 g
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihy-
dro-2H-chromen-6-yl)-N-benzylacetamide was obtained as grey solid,
which was used without further purification or characterization for
the subsequent reaction.
[0121] D) 4 ml of trimethylacetaldehyde was added to a solution of
11 g of the
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-benz-
ylacetamide obtained above in 200 ml methanol. 2.44 g of
NaBH.sub.3CN was added in several portions, and the resulting
suspension was stirred for 2 hours at 50.degree. C. After cooling
to room temperature, the reaction mixture was poured into 200 ml
water. The aqueous phase was extracted once with 150 ml EA, the
combined organic phases were dried over sodium sulfate, and the
solvent was largely evaporated at reduced pressure. Chromatography
of the remaining residue on silica gel (mobile phase: ethyl
acetate/cyclohexane 1:1 v/v) and drying of the product fractions on
a rotary evaporator yielded 10.8 g
N-benzyl-2-[3-hydroxy-2,2-dimethyl-4-(-
neopentylamino)-3,4-dihydro-2H-chromen-6-yl]acetamide as colorless
oil.
[0122] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 1.15 (s,
3H) 1.25 (t, 3H) 1.43 (s, 3H) 2.70 (q, 2H) 3.18-3.31 (3H) 3.55 (dd,
1H) 4.22 (dd, 1H) 4.30 (d, 2H) 5.51 (d, 1H) 5.81 (t, 1H) 6.56 (d,
1H) 6.67 (d, 1H) 6.96 (dd, 1H) 7.15 (m, 2H) 7.21-7.30 (3H) 7.32 (m,
2H) 7.84 (m, 2H); .sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]:
15.1 (q) 18.5 (q) 26.6 (q) 28.8 (t) 42.7 (t) 43.5 (t) 55.1 (d) 74.8
(d) 78.7 (s) 117.9 (d) 121.2 (s) 127.0 (s) 127.4 (d, 2 C) 127.5 (d,
3 C) 128.7 (d, 2 C) 128.9 (d, 3 C) 130.4 (d) 137.6 (s) 138.1 (s)
150.2 (s) 152.3 (s) 171.0 (s).
EXAMPLE 4
N-benzyl-2-{4-[[(4-ethylphenyl)sulfonyl](neopentyl)amino]-3-hydroxy-2,2-di-
methyl-3,4-dihydro-2H-chromen-6-yl}acetamide
[0123] 14
[0124] 4.1 ml 4-ethyl benzenesulfonyl chloride was added dropwise
to a solution of 10.8 g of the
N-benzyl-2-[3-hydroxy-2,2-dimethyl-4-(neopentyl-
amino)-3,4-dihydro-2H-chromen-6-yl]acetamide obtained above in
Example 3 and 5 ml triethylamine in 100 ml dichloromethane.
Dichloromethane was immediately substantially completely evaporated
at reduced pressure, and the resulting reaction mixture was stirred
for 90 minutes at 65.degree. C. and 180 bar. The entire batch was
poured into 150 ml water, and the aqueous phase was extracted once
with 200 ml ethyl acetate. The solvent was largely evaporated at
reduced pressure, the remaining residue was dried over sodium
sulfate, and the remaining residue was chromatographed on silica
gel (mobile phase: petroleum ether/ethyl acetate 3:1 v/v). Drying
the product fractions in an oil pump vacuum yielded 3.6 g of the
title compound (2 conformers) as colorless oil.
[0125] .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 0.75 (s,
9H) 1.15 (s, 3H) 1.26 (t, 3H) 1.46 (s, 3H) 2.36 (1H) 2.65-2.75 (2H)
2.90-3.40 (4H) 3.86 (d,d 1H) 4.39 (d, 2H) 4.76 (d, 1H) 5.50 (t, 1H)
6.48 (1H) 6.73 (d, 1H) 7.03 (dd, 1H) 7.15-7.35 (7H) 7.81 (m, 2H)
major; .sup.1H-NMR (400 MHz, CDCl.sub.3) .delta. [ppm]: 0.86 (s,
9H) 1.19 (s, 3H) 1.24 (t, 3H) 1.51 (s, 3H) 2.65-2.75 (2H) 2.81 (d,
1H) 3.25-3.60 (4H) 4.25-4.61 (4H) 4.60 (d,d 1H) 5.87 (t, 1H) 6.73
(d, 1H) 6.96 (dd, 1H) 7.15-7.35 (8H) 7.66 (m, 2H) minor;
.sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 15.1 (q) 17.9 (q)
27.0 (q) 28.8 (q, 3C) 28.8 (t) 32.0 (s) 43.0 (t) 43.7 (t) 56.3 (t)
59.0 (d) 71.2 (d) 79.1 (s) 118.4 (d) 120.7 (s) 127.0-130.4 (12C)
137.2 (s) 138.1 (s) 150.4 (s) 153.0 (s) 170.7 (s) major;
.sup.13C-NMR (101 MHz, CDCl.sub.3) .delta. [ppm]: 15.1 (q) 18.6 (q)
27.2 (q) 28.0 (q, 3C) 28.8 (t) 33.5 (s) 43.3 (t) 43.6 (t) 63.0 (t)
63.4 (d) 73.2 (d) 78.8 (s) 118.0 (d) 122.0 (s) 125.7-129.8 (12C)
137.3 (s) 138.3 (s) 150.2 (s) 151.8 (s) 171.3 (s) minor.
EXAMPLE 5
N-(4-chlorobenzyl)-2-(3-hydroxy-2,2-dimethyl-4-{[(3-methylphenyl)sulfonyl]-
amino}-3,4-dihydro-2H-chromen-6-yl)acetamide
[0126] 15
[0127] A) 175.6 g methyl-4-hydroxyphenylacetate and 128.9 g
phenylboronic acid were added to 3.5 l m-xylene. 88.9 g
3-methylbut-2-enal, and 130 ml of glacial acetic acid were added to
this mixture. The mixture was heated to 140.degree. C. in a
Dean-Stark apparatus under a nitrogen atmosphere until
approximately 70% of the phenol had reacted (approximately 48-72
hours). Then the reaction mixture was allowed to cool to room
temperature, the mixture was filtered, and the solvent was
evaporated at reduced pressure. The remaining residue was dissolved
in a 1:1 mixture of tetrahydrofuran (THF) and a 25%-strength
aqueous ammonia solution (v/v) and stirred for 2 hours. The THF was
largely evaporated off at reduced pressure and ethyl acetate was
added. The organic phase was separated, washed in succession with
an aqueous 1 N NaOH and a saturated aqueous common salt solution
and finally dried over sodium sulfate. The solvent was largely
evaporated at reduced pressure, and the remaining residue was
chromatographed on silica gel (mobile phase: n-hexane/EA 15:1 to
10:1 v/v). Drying of the product fractions in an oil pump vacuum
yielded 106 g methyl-(2,2-dimethyl-2H-chromen-6-yl)acetate as pale
yellow oil, which was used without further characterization for the
subsequent reaction.
[0128] B) 106 g of the methyl-(2,2-dimethyl-2H-chromen-6-yl)acetate
obtained above was dissolved in 900 ml THF. A solution of 57.6 g
LiOH in 900 ml water was added to this receiving solution, and the
mixture was stirred for 16 hours at room temperature. The THF was
largely evaporated at reduced pressure, and the aqueous residue was
acidulated by addition of an aqueous 6N hydrochloric acid solution.
The aqueous phase was extracted with ethyl acetate, and the
combined organic phases were washed in succession with a saturated
aqueous common salt solution and with water. The organic phase was
dried over sodium sulfate, and the solvent was then substantially
completely evaporated in a vacuum. 97.6 g
2,2-(dimethyl-2H-chromen-6-yl)-acetic acid was obtained, which was
used without further purification or characterization for the
subsequent reaction.
[0129] C) 14.0 g of the 2,2-(dimethyl-2H-chromen-6-yl)acetic acid
obtained above and 13.54 g EDC.times.HCl were dissolved in
dichloromethane at room temperature. 10.0 g of 4-chlorobenzylamine
was added dropwise to this receiving solution with stirring, and
the mixture was stirred for 16 hours at room temperature. Then the
reaction mixture was washed in succession with water, 1N aqueous
hydrochloric acid solution and saturated aqueous common salt
solution, and the organic phase was dried over sodium sulfate.
Evaporation of the solvent at reduced pressure and drying of the
remaining residue in an oil pump vacuum yielded 21.92 g of the
crude N-4-chlorobenzyl-2-(2,2-dimethyl-2H-chromen-6-yl)acetamide,
which was used without further purification or characterization for
the subsequent reaction.
[0130] D) 700 ml of a saturated aqueous sodium hydrogen carbonate
solution was added to a solution of 21.92 g of the
N-4-chlorobenzyl-2-(2,2-dimethy- l-2H-chromen-6-yl)acetamide
obtained above in 600 ml dichloromethane. A total of 31.6 g MCPBA
(72%) was added in portions to this receiving solution and stirred
for 16 hours at room temperature. The organic phase was separated,
washed twice with a 5%-strength aqueous sodium hydrogen carbonate
solution, dried over sodium sulfate and substantially completely
evaporated at reduced pressure. Drying in an oil pump vacuum
yielded
N-(4-chlorobenzyl)-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chr-
omen-6-yl)acetamide as crude oil, which was used without further
purification or characterization for the subsequent reaction.
[0131] E) The
N-(4-chlorobenzyl)-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[-
c]chromen-6-yl)acetamide obtained above was immediately poured into
a mixture of so much ethanol and 25%-strength aqueous ammonia
solution (6:5 v/v) that a 0.2 M solution of the compound was
obtained, and the resulting solution was stirred for 16 hours at
50.degree. C. Then the solution was allowed to cool to room
temperature, and the solvent was largely evaporated at reduced
pressure. The remaining residue was chromatographed on silica gel
(mobile phase: gradient dichloromethane/methanol/25%-strength
aqueous ammonia solution 97.5:2:0.5 to 90:9.5:0.5 v/v/v). Drying of
the product fractions yielded 7.3 g
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-4-chloro-
benzyl acetamide, which was used without further purification for
the subsequent reaction. The other regioisomer,
2-[3-amino-4-hydroxy-2,2-dime- thylchromen-6-yl]-N-4-chlorobenzyl
acetamide, was not observed.
[0132] F) 15 mg PS-methylpiperidine and a solution of 6.0 mg
3-methylbenzenesulfonyl chloride in 0.5 ml dichloromethane were
added in succession in a sample well of a sample plate for
automatic parallel synthesis to a solution of 9 mg of the
2-(4-amino-3-hydroxy-2,2-dimethyl--
3,4-dihydro-2H-chromen-6-yl)-N-4-chlorobenzyl acetamide obtained
above in 0.5 ml dichloromethane. The sample plate was shaken for 40
hours at room temperature, and then 20 mg AMPS was added to the
well. The plate was shaken for a further 16 hours at room
temperature before the liquid reaction phase was separated from the
resin, and the resin was washed twice with 1 ml portions of
dichloromethane. The solvent of the combined organic phases was
evaporated at reduced pressure, and the title compound was obtained
in a purity of 95% (determination by HPLC-MS), [M+H]+529.
EXAMPLE 6
N-butyl-2-{(4-[[(2,5-dimethoxyphenyl)sulfonyl](isobutyl)amino]-3-hydroxy-2-
,2-dimethyl-3,4-dihydro-2H-chromen-6-yl}acetamide
[0133] 16
[0134] A) 10.0 g 2,2-(dimethyl-2H-chromen-6-yl)acetic acid (for
preparation see Example 5B)), 9.67 g EDC.times.HCl and 5.41 g
n-butylamine were reacted according to the procedure set forth in
Example 5C). 17.5 g crude
N-(n-butyl)-2-(2,2-dimethyl-2H-chromen-6-yl)acetamide was obtained,
which was used without further purification or characterization for
the subsequent reaction.
[0135] B) 700 ml of a saturated aqueous sodium hydrogen carbonate
solution was reacted with a solution of 17.5 g of the
N-(n-butyl)-2-(2,2-dimethyl-- 2H-chromen-6-yl)acetamide obtained
above in 600 ml dichloromethane and 31.6 g MCPBA (72%) in a manner
corresponding to the procedure of Example 5D). The crude
N-butyl-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chromen-
-6-yl)acetamide was obtained, which was used immediately without
further purification or characterization for the subsequent
reaction.
[0136] C) The
n-butyl-2-(2,2-dimethyl-1a,7b-dihydro-2H-oxireno[c]chromen-6-
-yl)acetamide obtained above was immediately poured into a mixture
of so much ethanol and 25%-strength aqueous ammonia solution (6:5
v/v) that a 0.2 M solution of the compound was obtained and was
processed further in a manner corresponding to the procedure of
Example 5E). 7.4 g
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-(n-butyl-
)acetamide was obtained, which was used without further
purification for the subsequent reaction. The other regioisomer,
2-[3-amino-4-hydroxy-2,2--
dimethylchromen-6-yl]-N-(n-butyl)acetamide, was not observed.
[0137] D) 610 mg of the
2-(4-amino-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-c-
hromen-6-yl)-N-(n-butyl)acetamide obtained above was dissolved in
20 ml tetrahydrofuran (THF) and 220 .mu.l TMOF was added. Then 197
mg ethyl butyraldehyde was added to this receiving solution, and
the reaction mixture was shaken for 16 hours at room temperature.
The solvent was removed at reduced pressure, the remaining residue
was taken up with 20 ml methanol, 7.9 g PS--BH.sub.4 was added, and
the reaction mixture was shaken for a further 16 hours at room
temperature. The reaction mixture was then filtered, and the
reactive resin was washed with methanol. The combined filtrates
were largely evaporated at reduced pressure, the remaining residue
was dissolved in 20 ml dichloromethane, and 0.4 equivalents of a
known polymer-supported aldehyde (PS--CHO) and 0.6 equivalents of
AMPS were added in succession. The mixture was shaken again for 16
hours at room temperature, the liquid phase was filtered off from
the reactive resin, and the resin was subsequently washed with THF.
The combined liquid organic phases were evaporated at reduced
pressure, and 572 mg of 95% pure (determination by HPLC)
2-[4-(2-ethylbutylamino)-3-
-hydroxy-2,2-dimethylchromen-6-yl]-N-(n-butyl)acetamide was
obtained which was used without further purification or
characterization for the subsequent reaction.
[0138] E) 20 mg PS-methylpiperidine resin and a solution of 37.1 mg
3,5-dimethoxybenzenesulfonyl chloride in 0.4 ml dichloromethane
were added successively to in a sample well of a sample plate for
automatic parallel synthesis containing a solution of 14.8 mg of
the
2-[4-(2-ethylbutylamino)-3-hydroxy-2,2-dimethylchromen-6-yl]-N-(n-butyl)a-
cetamide obtained above in 0.6 ml dichloromethane. The mixture was
shaken for 168 hours at room temperature, the resin was filtered
out, and then 120 mg PS-AMPS was added to the filtrate. It was
shaken for a further 16 hours at room temperature before the liquid
reaction phase was separated from the resin, and the resin was
washed twice with 1 ml portions of dichloromethane. The solvent of
the combined organic phases was evaporated at reduced pressure, and
the title compound was obtained in a purity of 96% (determination
by HPLC-MS), [M+H]+591.
[0139] The compounds of Formula I listed in the following Table 6
can also be prepared according to the processes described in the
foregoing examples or analogously thereto.
6TABLE 6 Further compounds of Formula I: Ex. No. R.sup.1 R.sup.2
R.sup.3 R.sup.4 R.sup.5 R.sup.6 *C-3 *C-4 7 Me Me 4-ethylphenyl
neopentyl H benzyl S R 8 Me Me 4-ethylphenyl neopentyl H benzyl R S
9 Me Me 4-ethylphenyl H H (S)-tetra- S R hydronaphth-1-yl 10 Me Me
4-ethylphenyl neopentyl H phenylethyl trans 11 Me Me 4-ethylphenyl
H H benzyl S R 12 Me Me 4-ethylphenyl H H phenylethyl trans 13 Me
Me 3-fluorophenyl H H (S)-tetra- trans hydronaphth-1-yl 14 Me Me
phenyl H H (S)-tetra- trans hydronaphth-1-yl 15 Me Me
4-methylphenyl H H (S)-tetra- trans hydronaphth-1-yl 16 Me Me
4-ethylphenyl H --(CH.sub.2).sub.2N--C.sub.6H.sub.5 trans 17 Me Me
4-iodophenyl H n-butyl trans 18 Me Me 4-methylphenyl
cyclopropylmethyl H benzyl trans 19 Me Me 4-methylphenyl
3-methylbutyl H benzyl trans 20 Me Me 2,5-dimethoxyphenyl
2-ethylbutyl H n-butyl trans 21 Me Me 2,5-dimethoxyphenyl
2-ethylbutyl H 2-furylmethyl trans 22 Me Me 3-methoxyphenyl
n-pentyl H 1,2-dimethytpropyl trans 23 Me Me
4-trifluoromethylphenyl 3-methylbutyl H benzyl trans 24 Me Me
3-chloro-4-fluorophenyl 2-methylpropyl H n-propyl trans 25 Me Me
2-naphthyl 3-methylbutyl H benzyl trans 26 Me Me 4-biphenyl
3-methylbutyl H n-propyl trans 27 Me Me 3-methoxyphenyl
3-methylbutyl H 4-chlorobenzyl trans Notes to Table 6: trans =
trans position of the substituents at C-3 and C-4, but mixture of
the stereoisomers; Me = methyl; "S" and "R" each relate to the
absolute configuration on the corresponding carbon.
EXAMPLE I
Capsules Containing
2-(4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2-di-
methyl-3,4-dihydro-2H-chromen-6-yl)-N-1,2,3,4-tetrahydronaphth-1-yl
Acetamide
[0140] Capsules with the following composition per capsule were
prepared:
7 2-(4-{[(4-ethylphenyl)sulfonyl]amino}-3-hydroxy-2,2- 20 mg
dimethyl-3,4-dihydro-2H-chromen-6-yl)-N-1,2,3,4-
tetrahydronaphth-1-yl acetamide Corn starch 60 mg Lactose 300 mg
Ethyl acetate q.s.
[0141] The active substance, the corn starch and the lactose were
processed into a homogenous pasty mixture using the ethyl acetate.
The paste was ground, and the resulting granules were placed on a
suitable tray and dried at 45.degree. C. in order to remove the
solvent. The dried granules were passed through a crusher and mixed
in a mixer with the following additional auxiliaries:
8 Talcum 5 mg Magnesium stearate 5 mg Corn starch 9 mg and then
filled into 400 mg capsules (=capsule size 0).
[0142] The foregoing description and examples have been set forth
merely to illustrate the invention and are not intended to be
limiting. Since modifications of the described embodiments
incorporating the spirit and substance of the invention may occur
to persons skilled in the art, the invention should be construed
broadly to include all variations within the scope of the appended
claims and equivalents thereof.
* * * * *