U.S. patent application number 10/493602 was filed with the patent office on 2004-12-16 for method and devices for the cross-referencing of identification of object supports for microtomised analytical samples and for the generation of said identification.
Invention is credited to Heid, Hans L., Novoa, Jose.
Application Number | 20040253662 10/493602 |
Document ID | / |
Family ID | 7705024 |
Filed Date | 2004-12-16 |
United States Patent
Application |
20040253662 |
Kind Code |
A1 |
Heid, Hans L. ; et
al. |
December 16, 2004 |
Method and devices for the cross-referencing of identification of
object supports for microtomised analytical samples and for the
generation of said identification
Abstract
The invention relates to a method and devices for the
cross-referencing of identification (1) of object supports (2), for
microtomised analytical samples still to be mounted thereon, with
identification information (3) for a support (4) of a tissue sample
(5) which is not yet microtomised. The conventional problem of
cross-referencing is improved in a simple manner, whereby the
identification information (3) for the support (4) is automatically
generated during the very allocation in the microtome (6) and an
identification (1), corresponding thereto, is automatically
transferred to at least one object support (2) and that finally
said object support (2), provided with the identification (1) is
given for the application of the microtomised tissue sample at the
moment when a microtomised tissue sample must be applied to an
object support (2).
Inventors: |
Heid, Hans L.; (Bammental,
DE) ; Novoa, Jose; (Heidelberg, DE) |
Correspondence
Address: |
PAUL J VINCENT
KOHLER SCHMID MOEBUS
D-70565 STUTTGART
DE
|
Family ID: |
7705024 |
Appl. No.: |
10/493602 |
Filed: |
April 26, 2004 |
PCT Filed: |
October 26, 2002 |
PCT NO: |
PCT/EP02/11983 |
Current U.S.
Class: |
435/40.52 ;
422/400 |
Current CPC
Class: |
G01N 2035/00782
20130101; Y10T 436/11 20150115; G01N 2035/00148 20130101; Y10T
436/25 20150115; C12Q 1/04 20130101; G01N 1/312 20130101; G01N 1/06
20130101; G01N 35/00732 20130101; Y10T 156/108 20150115 |
Class at
Publication: |
435/040.52 ;
422/102 |
International
Class: |
G01N 001/30 |
Foreign Application Data
Date |
Code |
Application Number |
Nov 8, 2001 |
DE |
101 54 843.5 |
Claims
1-29. (cancelled).
30. A method for allocating an identification to microtomised
sample tissue holders presented to a technician working on a
microtome and manually mounting a microtomised sample tissue onto
the sample tissue holder, the method comprising the steps of: a)
assigning identification information to a support of a tissue
sample prior to complete microtomisation thereof; b) automatically
detecting said identification information during disposal of said
support in the microtome; c) automatically transferring at least a
portion of said identification information to at least one sample
tissue holder; and d) providing, following step c), only those
sample tissue holders having said automatically transferred
identification information to the technician at a time during which
the micromotised tissue sample is to be manually mounted to said
tissue holder.
31. The method of claim 30, wherein, for each production of a
microtomised tissue sample, said identification information is
detected and said tissue holder is provided with said
identification information.
32. The method of claim 30, wherein said identification information
transferred to said at least one tissue holder is identical to said
identification information on said sample support.
33. The method of claim 30, wherein said identification information
on said sample support contains further information in addition to
an identification of an origin of the tissue sample.
34. The method of claim 33, wherein said further information is
allocated by means of an information distribution key, said further
information individualizing a microtomised tissue sample of a
particular sample holder.
35. The method of claim 33, wherein said further information
contains processing instructions for the microtomised tissue sample
of said tissue holder.
36. The method of claim 33, wherein said further information is
evaluation information for the microtomised tissue sample on said
tissue holder.
37. The method of claim 34, wherein said further information
characterizes said information distribution key.
38. A device for allocating an identification to microtomised
sample tissue holders presented to a technician working on a
microtome and manually mounting a microtomsed sample tissue onto
the sample tissue holder, the device comprising: means for
assigning identification information to a support of a tissue
sample prior to complete microtomisation thereof; means for
detecting said identification information during disposal of said
support in the microtome; means for transferring at least a portion
of said identification information to at least one sample tissue
holder; and means for providing only those sample tissue holders
having said automatically transferred identification information to
the technician at a time during which the microtomised tissue
sample is to be manually mounted to said tissue holder.
39. The device of claim 38, wherein said transferring means
comprises an identification means for providing said tissue holder
with an identification label following information transfer between
said detecting means and said identification means, wherein said
providing means comprises a delivery means installed at the work
place of the microtome for removal, by the technician working on
the microtome, of tissue holders having said identification label
prior to manually mounting of the microtomised tissue sample.
40. The device of claim 39, wherein said detecting means detects
the identification information after positioning said support in
the microtome to effect identification of at least one tissue
holder.
41. The device of claim 39, wherein, when a microtomised tissue
sample is produced, a command is issued to said identification
means to label said tissue holder.
42. The device of claim 39, further comprising a data carrier for
storing said identification information, wherein said
identification information is associated with said support via an
identfication code.
43. The device of claim 39, wherein said identification information
is located on an information carrier which is physically connected
to said support.
44. The device of claim 39, wherein said delivery means interrupts
provision of said tissue holder having said identification label
when a new tissue support is placed into the microtome.
45. The device of claim 39, further comprising a controller for
carrying out identification of said tissue holder.
46. The device of claim 45, wherein said controller comprises a
command input for determining a type and/or number of
identifications.
47. The device of claim 39, further comprising a depot for
non-labelled tissue holders.
48. The device of claim 45, wherein said controller predetermines a
number of tissue holders to be provided with said
identification.
49. The device of claim 48, wherein said controller manually
increased said number of tissue holders to be provided with said
identification.
50. The device of claim 45, wherein said controller extracts
information about a number and/or type of labelling from said
identification information.
51. The device of claim 39, further comprising a display means for
displaying information and/or processing steps.
52. The device of claim 43, further comprising means for holding
said support, wherein said detecting means, said support and said
support holding means are disposed and structured such that said
detecting means can read said identification information from a
read field disposed on said support.
53. The device of claim 52, wherein said support and said support
holding means cooperate such that clamping jaws of said support
holding means are located outside of said read field of a clamped
said support.
54. The device of claim 43, wherein said assigning means comprises
means for producing said identification information on an
information carrier associated with said support, said
identification information containing identification as to origin
of the tissue sample, an information distribution key, and
information which provides an individual information content for
identification of several tissue holders having microtomised tissue
samples which are derived from a same, not yet microtomised tissue
sample.
55. The device of claim 54, wherein said means for producing said
identification information loads said identification information
onto a separate data carrier and provides same and said information
carrier of said support with an identification code for mutual
allocation.
56. The device of claim 54, wherein said means for producing said
identification information and said information carrier are
designed such that said information carrier can be loaded with a
entire amount of said identification information.
57. The device of claim 54, wherein an information content of said
tissue holder comprises processing instructions for the
microtomised tissue samples.
58. The device of claim 54, wherein an information content of said
tissue holder contains evaluation instructions for the microtomised
tissue samples.
59. The device of claim 54, wherein an information content of said
tissue holder comprises device control instructions which can be
automatically detected and processed.
Description
[0001] The invention concerns a method for cross-referencing the
identification of object supports for microtomised tissue samples
to be disposed thereon, with cross-reference to an identification
information of a support of an associated, not yet microtomised
tissue sample.
[0002] The invention also concerns a device for carrying out such a
method and a device for preparing to carry out the method to
identify object supports of microtomised tissue samples with
cross-reference to identification information of a support of a not
yet microtomised tissue sample, wherein the support is provided
with an information carrier for cross-reference, and the device
comprises a means for generating the identification
information.
[0003] Examination of tissue samples from patients is a routine
procedure in histological technology. The most common method
consists of embedding the tissue samples in paraffin, cutting them
with microtomes, staining them in a further process, covering them
with a cover glass and supplying them to a microscope for
diagnostic evaluation. In modern laboratories, the patient samples
are automatically processed during the individual steps. The
samples are initially prepared through the following steps: fixing,
dehydration, clearing with an intermediate medium, and embedding in
paraffin in an embedding device. Production of a paraffin block is
realized in a pouring station. Microscopic, thin slices are
produced through cutting the paraffin block, with an embedded
tissue sample, in a microtome. The thin slice thereby obtained is
stained and contrasted in several steps in a staining device. After
covering the thin slice with a cover glass on a cover glass
covering device, the thin slice obtained from the patient sample is
ready for evaluation under a microscope.
[0004] One problem consists in maintaining unique identification of
the tissue samples to prevent confusion and loss of samples
throughout all of the processing steps. Some progress has been
achieved in this regard. In modern processing, immediately after
cutting, the tissue sample is inserted into a so-called tissue
cartridge which is closed and secured by a lid. In addition to good
liquid flow properties, modern tissue cartridges also offer
distinct features to prevent loss of the sample and to provide
unique identification. Towards this end, modern cartridges have a
writing or printing surface of sufficient size and surface quality
to permit writing or printing with sufficient adhesion and
durability during the subsequent processing. In this fashion, the
sample can be clearly identified in the identified cartridge during
embedding of the tissue. In the subsequent step, i.e. pouring the
paraffin block around the tissue sample, the risk of sample
confusion is still sufficiently prevented, since the same
identified cartridge serves as support of the paraffin block with
tissue sample, wherein the sample is connected to the identified
cartridge via the solidified paraffin block.
[0005] In the next step, microscopic thin slices of the tissue
sample are produced on a microtome. Towards this end, the paraffin
block with embedded tissue sample and connected cartridge support
part are clamped into the sample holder of a microtome and
microtomised. Thin slices are thereby produced on the back of the
microtome knife or on the edge of one-way blade holders, which are
transferred manually, using a brush and tweezers, to a warm water
bath for stretching the slices. The stretched, thin slices are then
placed on a conventional, glass object support. The labelling field
of the object support must now have the same allocating information
concerning the tissue as is written on the tissue cartridge serving
as the support. Conventionally, several usable thin slices are
produced from one tissue sample and are distributed on several
object supports. This means that several object supports must
contain the same identification as to origin of the tissue
sample.
[0006] The object support can be labelled by hand-writing as soon
as the thin slices have been produced. The patient tissue
information provided on the cartridges is thereby transferred onto
one or more object supports through hand-writing. Clearly, this
method increases the danger of transfer errors and therefore offers
little protection from confusion and errors. Additionally, the
hand-written information must be read and further processed by
other persons at a later time.
[0007] U.S. Pat. No. 4,276,253 discloses mounting an identification
on the sample which is maintained for each microtomised sample
tissue during cutting, i.e. is reproduced. There is no allocation
problem since the microtomised tissue sample is already
characterized during cutting. This requires extensive
identification of samples using shaped, elongated labels which are
also cut. This requires an increase in size of the paraffin block
being cut and also entails time-consuming embedding of these
labels. The considerable technical effort renders practical
applications doubtful.
[0008] Further documents (WO 00/62035, U.S. Pat. No. 5,854,075;
U.S. Pat. No. 5,746,855 and DE 100 10 140 A1) describe automated
processes which eliminate allocation problems. Such full automation
is problematic due to the extremely thin slices and can only be
realized with considerable technical effort.
[0009] A method and devices of the above-mentioned type with manual
cross-reference are also known, wherein one or more object supports
are labelled with the same information at the same time when the
tissue cartridge, which serves as support, is labelled at the start
of the sample preparation process. This reduces the risk of
confusion, in particular when the writing is carried out
automatically with a coupled cartridge and object support printing
system. This method is disadvantageous in that the previously
labelled object supports cannot accompany the patient sample with
tissue cartridge as one integrated unit in the subsequent steps.
This means that, after microtomising of a tissue sample, the
matching previously labelled object supports must be found and
identified through manual comparison of the written information. A
further disadvantage is the fact that the number of object supports
required for each tissue sample cannot be exactly predetermined at
the time of labelling since, in addition to the specifications of
the doctor, this number also depends on the assessment of the
expert carrying out microtomisation. In practice, it is very
difficult to adhere to a systematology which ensures
cross-reference of the object support and tissue sample of the
patient by allocating previously labelled object supports, since
the samples must be relocated several times during the individual
preparation steps. For this reason, the object support must
disadvantageously be located in intermediate positions and
comparative readings are required to provide a new
cross-reference.
[0010] Pre-fabricated object supports with corresponding
identification are difficult to allocate and would also have to be
reproduced or be provided in excessive numbers when the expert
providing the thin slices thereby realizes that more microtomised
tissue samples are required than expected by the doctor. This
problem increases when the object support identification must
contain further information concerning different processing and
evaluation, in addition to the cross-reference to the patient.
[0011] It is therefore the underlying purpose of the present
invention to provide a safe and efficient identification method for
object supports and provide devices therefor which simplify the
described cross-referencing problems in histological technology, in
particular those occurring during manual cross-referencing.
[0012] This object is achieved with a method of the above-mentioned
type in that the identification information which belongs to the
support is automatically detect during arrangement thereof in the
microtome, and an identification to be associated therewith is
automatically transferred to at least one object support and
subsequently, with only this object support bearing the
identification being provided at the work place of the microtome to
the technician working on the microtome for the manual mounting of
a microtomised tissue sample at the time when the microtomised
tissue sample must be disposed on an object support.
[0013] This object is achieved with respect to the device for
carrying out this method in that the device comprises a detecting
means for detecting the identification information while the
support comprising a non-microtomised tissue sample is disposed in
the holding means of a microtome, and a labelling means for
providing an object support with an identification, a transfer
means for information transfer between the detecting means and the
labelling means and a discharge means installed at the work place
of the microtome for removal, by the technician working on the
microtome, of object supports provided with an identification, for
manual mounting the microtomised tissue sample.
[0014] The inventive method assumes the complete task of the expert
producing the microtomised samples, i.e. the thin slices, of
allocating these samples to the non-microtomised tissue sample. In
this connection, non-microtomised tissue samples also include such
tissue samples from which the first thin slices have been cut off.
The automated detection mainly permits detection thereof when the
support including tissue sample is located in the microtome. This
is not possible in a reliable manner using the human eye, since the
identification cannot be easily read due to the restricted space.
The invention avoids detection outside of the microtome, since one
cannot guarantee that the detected tissue sample is actually
clamped. Labelling errors of the object supports are eliminated
through automatic detection of the identification information
during clamping of the support as well as through automatic
transfer and identification. Transfer may be effected electrically,
optically, through infrared, radio waves or in another manner.
[0015] The reliability is further ensured, when the expert has only
one such object support at a time whose identification as to origin
coincides with the microtomised tissue sample, since at the moment
during which the expert must place the microtomised tissue sample
onto an object support, only the object support having the correct
identification is available. All human allocation is avoided. The
avoidance of any such allocation also eliminates the associated
occurrence of errors. Even when the expert interrupts microtomising
of a sample to process another sample in the meantime, no
allocation problems can occur, since only one object support is
available at a time: namely, the object support having an
identification associated with the identification information of
the tissue sample located in the microtome. Identification
information and identification can thereby be connected physically
or electronically to the support and the object support. In the
latter case, a physically allocated identification code simplifies
electronic allocation. The latter solution is to be preferred for
large amounts of data or when a physical information link with
extensive data is problematic due to the required processing.
[0016] The inventive device provides the means required to carry
out the inventive method. These means provide that production and
provision of the correctly labelled object support--and only this
support--is possible automatically and "just in time" at the
location where required and in the desired amount.
[0017] Clearly, the method and device are not restricted to the
above-mentioned technique for providing microtomised tissue
samples, since the inventive solution does not depend on the manner
in which the tissue sample is treated and cut. The device may
comprise a microtome, with the knife being moved or with the knife
being fixed and the sample being moved. One of these elements can
be moved on a slide or a rotating disc and be manually or
automatically driven. Of course, a laser beam or another method for
producing tissue slices can also be used. The tissue slices may be
human, animal, or plant tissue slices.
[0018] In the device of the above-mentioned type for preparing an
identification of object support, this means, the information
carrier, and a data carrier associated with the support are
designed to produce identification information which contains, in
addition to identification as to the origin of the tissue sample,
an information distribution key and information which provides an
individual information content for identification of several object
supports with microtomised tissue samples which are derived from
the same, not yet microtomised tissue sample.
[0019] This device prepares labelling of the object support in that
the required information is stored as identification information
such that it is linked mechanically or electronically with the
support of the sample tissue such that, on this basis, the
above-mentioned method can be carried out with the above-mentioned
device, wherein this device for preparing the identification
includes applications with which the identification information
contains cross-reference information providing individual
information content for individual object supports having thin
slices derived from the same tissue sample. In this manner, not
only correct allocation of the identification of object supports
for microtomised tissue samples with the corresponding not yet
microtomised tissue samples is obtained but it is also ensured that
a desired number of tissue slices is produced on object supports
having the desired additional information.
[0020] Further developments of the method and of the mentioned
devices and the inventive processing device are described
below.
[0021] The inventive method is advantageously further developed
such that every time a microtomised tissue sample is produced, the
identification information is detected and an object support having
an identification is produced and offered. This ensures that an
object support is provided for each microtomised tissue sample. It
is thereby possible to assure that the object support is produced
only when the expert working on the microtome regards the
microtomised tissue sample as useful and initiates production of
the identified object support.
[0022] In the simplest case, the identification of the at least one
object support contains the same identification information which
is associated with the non-microtomised tissue sample. In most
cases, only reference is thereby made to the origin of the
non-microtomised tissue sample or to the corresponding patient. The
identification information may contain further information in
addition to the identification as to origin. Such information may
include pre-treatment of the tissue sample, date or place of
removal or organ of removal. Further information is feasible, as
will be exemplified below.
[0023] If different treatment or different evaluation of the
microtomised tissue samples is required or their sequence is to be
fixed, it is advisable to load the identification of the object
support with further information, in addition to identification as
to origin, using an information distribution key to individualize
individual microtomised tissue samples on the object supports,
which are derived from the same non-microtomised tissue sample.
This permits further treatment and evaluation on the basis of the
identification and avoids the necessity of separately adding such
information to the object supports. This also reduces associated
problems in histological technology. The information processing key
and the further information are advantageously contained in the
identification information and thereby physically or electronically
connected to the support and thereby to the non-microtomised tissue
sample.
[0024] The device for carrying out the above-mentioned method may
be designed in different ways. The detecting means may be designed
such that it detects the identification information after
positioning of the support in the microtome and initiates
identification of at least one object support. The detecting means
may also be designed such that the identification information is
detected when a microtomised tissue sample is produced and a
command is issued to the identification means to initiate labelling
of an object support. Continuous detection of the identification
information and labelling of object supports is also possible if
required e.g. by pressing a button.
[0025] Identification information and identification may, of
course, be designed in the most different of ways. It may be in
writing which can be read by a person, or be mechanically evaluated
such as e.g. a bar code, or also be a magnetic or electronic
storage medium of any type. The identification information or
identification can thereby be provided on a separate data carrier
which can be allocated to a support or an object support by means
of an identification code. In this case, it must of course be
ensured that only the correct allocation is admissible. This
solution is advantageous when additional data must be allocated,
usually in the form of additional identification information. It is
often sufficient and straightforward to load the identification
information on an information carrier which is physically connected
to the support.
[0026] To prevent an object support of the previously processed
tissue sample from remaining in the discharge means thereby
producing an allocation error, it is advisable to design the
discharge means such that it interrupts presenting an object
support provided with an identification when a new support is
placed in the microtome. Towards this end, the discharge means may
reject or withdraw the remaining object support to write new
information on it.
[0027] Should the device perform functions in addition to the pure
copying of identification as to origin, it is advantageous to
provide it with a controller for labelling the object support. If
such a controller is provided it is moreover advisable to provide
the device with a command input for determining the type and/or
number of identifications. This command input maybe a manual
command input or a command input through identification
information. An external command input via a data line connected to
a computer or command input via a data carrier is also
feasible.
[0028] The device, preferably the identification means, should
comprise a depot for non-identified object supports which should be
large enough to be able to produce labelled object supports of
sufficient number without requiring constant recharging.
[0029] The controller may be designed such that the number of the
object supports to be provided with an identification can be
predetermined. This corresponds to the fact that the doctor
generally decides how many microtomised tissue samples are required
on object supports. Moreover, the controller should be designed
such that the number of object supports to be labelled can be
manually increased. This addresses the fact that the expert working
on the microtome will often realize that more object supports are
required than are predetermined, either because the quality of the
tissue sample is doubtful or the expert can see already from the
thin slice that it is peculiar and more thin slices are required
for diagnosis.
[0030] As mentioned above in connection with command input, the
controller may obtain the information in the most differing of
ways. In an advantageous embodiment which minimizes errors, the
controller is designed to receive information about the number
and/or type of identification from identification information
containing this information.
[0031] In particular, if the device comprises a command input
and/or controller, it is advisable to also provide it with a
display means for displaying information and/or work steps which
will inform the microtomising expert about the number and type of
thin slices to be produced and about their associated information.
This provides additional control and supplementary inputs.
[0032] In a practical embodiment of the device, the detecting
means, the supports and the holding means are designed and disposed
such that the detecting means can read the identification
information from a read field of the support. Towards this end, the
support and holding means may be designed and mutually adapted such
that the clamping jaws of a holding means designed as clamping
means are located outside of the read field of a clamped support.
This may be achieved through corresponding arrangement and design
of the detecting means or through modification of the clamping jaws
compared to the previous clamping devices, wherein the aim is to
provide enough space on the read field for a detecting means. The
term "read field" means of course that not only optical information
but also magnetically or electronically stored information can be
read.
[0033] The device for preparing identification of object supports
through preparing identification information to be associated with
the supports can be designed in the most differing of ways. The
information carriers may thereby have widely varying designs and
the means for generating identification information may also have
correspondingly different designs. The means for generating
identification information may be designed such that the latter is
loaded onto a separate data carrier, wherein this data carrier or a
certain data content of a data carrier and the information carrier
of the support of the tissue sample are provided with an
identification code for mutual cross-reference. This design is
particularly advantageous when the sample tissues which are not
microtomised and/or microtomised must be associated with a large
amount of data and the support is not large enough for mounting a
storage medium of sufficient space thereon or when the intended
processing of a sample does not accept such a storage medium. In an
alternative embodiment, the means and the information carrier are
designed such that the entire identification information can be
loaded onto the information carrier.
[0034] The object supports may obtain different information
contents by means of the information distribution key. It is
possible to e.g. provide the object supports with different
processing instructions for the microtomised tissue samples or the
information content of the object support may contain individual
evaluation information for the microtomised tissue samples.
[0035] Finally, the above-mentioned device can also be used
together with the method and the device for carrying out the method
in a largely automated fashion. Towards this end, the
above-mentioned information content is advantageously designed as
device control instructions which can be mechanically read and
realized.
[0036] For realization, a device for processing microtomised tissue
samples disposed on object supports is advantageous which comprises
a means for detecting identifications of object supports and a
device controller which is designed to realize device controller
instructions containing the identifications of the object supports.
Such automation which is based on the above-mentioned inventive
measures facilitates cross-referencing, in the present case, for
further processing.
[0037] Further designs and developments are of course feasible, in
particular, many method features can be realized as device features
and vice versa.
[0038] The invention is explained below with reference to
embodiments of the device.
[0039] FIG. 1 shows an embodiment of a device for carrying out the
method;
[0040] FIG. 2a shows an embodiment of a support designed as
cartridge including read field;
[0041] FIG. 2b shows a cartridge lid for the above-mentioned
cartridge;
[0042] FIG. 2c shows a cartridge with identification information of
different design;
[0043] FIG. 3a shows a conventional holding means;
[0044] FIG. 3b shows a holding means designed for arrangement of a
detecting means; and
[0045] FIG. 4 shows an embodiment of a device for producing
identification information.
[0046] FIG. 1 shows an embodiment of the inventive device for
carrying out the inventive method. A microtome 6 includes holding
means 8 into which a support 4 is clamped on a displaceable slide,
the support comprising a not yet microtomised tissue sample 5. The
not yet microtomised tissue sample 5 is displaced in the direction
of a double arrow 21 to produce thin slices via the knife 20 of the
microtome 6. The slide guidance and adjustment to obtain the slice
thickness are substantial functions of the microtome 6. These thin
slices are the microtomised tissue samples which are disposed on
the delivery 22 and removed as described above to be supplied to a
water bath 23. The warm water bath 23 smoothes the microtomised
tissue samples, i.e. the thin slices, which can then be disposed
onto an object support 2.
[0047] The invention solves the problem that the identification 1
of the object supports 2 of the identification information 3 of the
not yet microtomised tissue sample 5 is reliably and efficiently
allocated. Towards this end, the microtome 6 has a detecting means
7 which detects the identification information 3 of the support 4
and transfers it to an identification means 9 for object supports 2
via a transfer means 10. In correspondence with the identification
information 3, this identification means 9 generates identification
1 of an object support 2 which can then be removed at the delivery
means 11 to receive the microtomised tissue sample from the water
bath 23. In this manner, cross-reference of the identification 1 on
the object support 2 with the identification information 3 of the
support 4 is always ensured. It is essential that production of the
identification 1 of the object support 2 is always connected to the
identification information 3 of the support 4 located in the
holding means 8. When the support 4 is removed, no identification 1
which could be associated therewith can be produced. When a new
support 4 is clamped or when the old support 4 is removed, any
labelled object supports 2 must be removed from the delivery means
11 and, of course, from the region of the work place of the
microtome 6 to ensure that they do not remain and are possibly
associated with a microtomised tissue sample 5 which does not
correspond to its identification information 3. Towards this end,
the labelling means 9 is preferably designed such that it withdraws
unused labelled object supports 2 before processing of a new, not
yet microtomised tissue sample 5.
[0048] The embodiment comprises a transfer means 10 which is
designed e.g. as infrared transmission. A corresponding receiver is
disposed on the labelling means 9. Reference numeral 13' indicates
that the detected identification information 3 may also include a
command input 13' which is also transmitted through the transfer
means 10. Such a command input facilitates individualized labels 1
of object supports 2 in a manner described above. Alternatively,
manual command input 13 or external command input 13" is also
feasible. The latter may be connected e.g. to a computer. The
operator may be informed about the number and type of
identifications 1 etc. through a display means 14.
[0049] The labelling means 9 is disposed in a housing which also
contains a control 12 which e.g. processes commands or converts
identification information 3 into individualized identifications 1
of object supports 2 (see description above). The identification
means 9 furthermore comprises a depot 16 into which non-labelled
object supports 2 can be introduced to provide sufficient
supply.
[0050] FIG. 2a shows an embodiment of a support 4 designed as
cartridge 24 including identification information 3 which is
disposed on an information carrier 15 designed as read field 15'.
Of course, other embodiments of an information carrier 15 are
feasible such as electromagnetic strips, an electronic storage or a
bar code, as shown in FIG. 2c. The non-microtomised tissue samples
5 are placed into the cartridges 24 and closed with a lid 25, shown
in FIG. 2b. The tissue samples are then treated as described above.
The non-microtomised tissue sample 5 is finally soaked with
paraffin and connected to the cartridge 24 as a paraffin block such
that the latter serves as support 4 for the non-microtomised tissue
sample 5.
[0051] FIG. 3a shows a conventional holding means 8 which places
the above-described support 4, i.e. the cartridge 24, which is
connected to the not yet microtomised tissue sample 5, on the
microtome 6 for producing thin slices. The conventional holding
means 8 has clamping jaws 17 which hold the cartridge 24 at the
read field 15' and on the opposite side. This complicates
accommodation of a detecting means 7. This problem may be solved
when the holding means 8 has a design as shown in FIG. 3b, i.e.
such that the clamping jaws 17 hold the cartridge 24 on its
longitudinal sides. This is, of course, only one example. Many
other possibilities are feasible for accommodating the detecting
means 7.
[0052] FIG. 4 shows an embodiment of a device 18 for preparing
identification 1 of object supports 2. Preparation is realized by
providing supports 4 with identification information 3 which can be
converted into corresponding identification 1 of object supports 2
through control 12 of the labelling means 9 as described in detail
above. The device 18 therefore comprises a means 19 for producing
identification information 3 and supports 4 with information
carriers 15, 15'. The means 19 for producing such identification
information 3 has an input 27 which is designed e.g. as keyboard,
and a display 28, e.g. a screen. All data can be entered to load
the information carrier 15 of a support 4 with corresponding
identification information 3. Alternatively, this process can of
course be carried out by providing a data carrier receptacle 30
into which a data carrier, e.g. a disc, is inserted which contains
the corresponding identification information 3. The means 19 will
then produce the identification information 3 on the support 4 in
correspondence with this data. A data line to a computer would also
be feasible to produce identification information 3. For a good
work flow, a depot 26 for supports 4 without identification
information 3 is provided.
[0053] This device 18 must, of course, have a special means for
joining the support 4, the identification information 3 and the
correct tissue sample 5. In accordance with the present invention,
this is the last required critical task which must be performed
when a tissue sample 5 is allocated for the first time following
removal from the body. The invention prevents confusion concerning
sample identification during all further processing and evaluation
of the tissue sample.
[0054] The figures show, of course, only some of all possible
designs of the inventive device. The figures illustrate use with a
conventional microtome 6 which may also have a different design.
The labelling means 9 may be built on or have another design.
[0055] Method and Devices for the Cross-Referencing of
Identification of Object Supports for Microtomised Analytic
Samples.
[0056] List of Reference Numerals
[0057] 1 Identification of an object support for microtomised
tissue samples
[0058] 2 Object support
[0059] 3 Identification information, allocated to a support for not
yet microtomised tissue samples
[0060] 4 Support
[0061] 5 Not yet microtomised tissue sample
[0062] 6 Microtome
[0063] 7 Detecting means
[0064] 8 Holding means
[0065] 9 Identification means
[0066] 10 Transfer means
[0067] 11 Delivery means
[0068] 12 Controller
[0069] 13, 13', 13" Command input
[0070] 13 Manual command input
[0071] 13' Command input through identification information
[0072] 13" External command input or command signals via a data
carrier
[0073] 14 Display means
[0074] 15 Information carrier
[0075] 15' Read field
[0076] 16 Depot for unidentified object supports
[0077] 17 Clamping jaws
[0078] 18 Device for preparing identification of object
supports
[0079] 19 Means for producing identification information
[0080] 20 Knife of microtome
[0081] 21 Double arrow: displacement of the holding means to
produce thin slices
[0082] 22 Deposit of the thin slices
[0083] 23 Water bath
[0084] 24 Cartridge
[0085] 25 Cartridge lid
[0086] 26 Depot for support without identification information
[0087] 27 Input (keyboard)
[0088] 28 Display (screen)
[0089] 29 Output for support with identification information
[0090] 30 Data carrier reception
* * * * *