U.S. patent application number 10/774668 was filed with the patent office on 2004-08-12 for somatostatin antagonists and agonists that act at the sst subtype 2 receptor.
This patent application is currently assigned to Pfizer Inc. Invention is credited to Cole, Bridget M., Hay, Bruce A., Ricketts, Anthony P..
Application Number | 20040157834 10/774668 |
Document ID | / |
Family ID | 26940124 |
Filed Date | 2004-08-12 |
United States Patent
Application |
20040157834 |
Kind Code |
A1 |
Hay, Bruce A. ; et
al. |
August 12, 2004 |
Somatostatin antagonists and agonists that act at the SST subtype 2
receptor
Abstract
Compounds according to the formula A-Z-W as herein described,
wherein A is selected from the groups consisting of:
A'-(CH.sub.2).sub.n--, A'-(CH.sub.2).sub.nSO.sub.2--, and
A'-(CH.sub.2).sub.nCO--, where n is 0 to 4; and A.dbd. is selected
from (a) (C.sub.6-C.sub.10)aryl-, or (b)
(C.sub.1-C.sub.9)heteroaryl-; which groups may be optionally
substituted; and pharmaceutically acceptable salts, solvates or
hydrates thereof; pharmaceutical compositions thereof; and methods
useful to facilitate secretion of growth hormone(GH) in
mammals.
Inventors: |
Hay, Bruce A.; (East Lyme,
CT) ; Cole, Bridget M.; (Stonington, CT) ;
Ricketts, Anthony P.; (Stonington, CT) |
Correspondence
Address: |
PFIZER INC.
PATENT DEPARTMENT, MS8260-1611
EASTERN POINT ROAD
GROTON
CT
06340
US
|
Assignee: |
Pfizer Inc
|
Family ID: |
26940124 |
Appl. No.: |
10/774668 |
Filed: |
February 9, 2004 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10774668 |
Feb 9, 2004 |
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09953785 |
Sep 17, 2001 |
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6720330 |
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60249514 |
Nov 17, 2000 |
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Current U.S.
Class: |
514/224.2 ;
514/230.5; 514/241; 514/248; 514/249; 514/251; 514/263.1; 514/303;
514/310; 514/311; 514/340; 514/362; 514/364; 514/367; 514/372;
514/375; 514/381; 514/383; 514/398 |
Current CPC
Class: |
C07K 5/06191 20130101;
A61K 38/00 20130101; C07K 5/06139 20130101 |
Class at
Publication: |
514/224.2 ;
514/230.5; 514/248; 514/249; 514/241; 514/263.1; 514/251; 514/310;
514/311; 514/303; 514/367; 514/375; 514/362; 514/364; 514/340;
514/372; 514/381; 514/383; 514/398 |
International
Class: |
A61K 031/5415; A61K
031/538; A61K 031/53; A61K 031/495; A61K 031/525; A61K 031/52 |
Claims
1. A compound according to the formula A-Z-W (formula I) or a
pharmaceutically acceptable salt, solvate, or hydrate thereof;
wherein A is selected from the groups consisting of
A'-(CH.sub.2).sub.n--, A'-(CH.sub.2).sub.nSO.sub.2--, and
A'-(CH.sub.2).sub.nCO--, where n is 0 to 4; and A' is selected from
(a) (C.sub.6-C.sub.10)aryl-, selected from phenyl or naphthyl; or
(b) (C.sub.1-C.sub.9)heteroaryl-, selected from the group
consisting of furyl-, thienyl-thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl- quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-; wherein said A' group (a) or (b)
is optionally substituted by zero to seven, preferably zero to five
groups, each independently selected from: hydroxy, halo, amino,
trifluoromethyl-, carboxy, (C.sub.1-C.sub.6)alkoxy-- ,
(C.sub.1-C.sub.6)acyloxy-, (C.sub.1-C.sub.6)alkylamino-,
((C.sub.1-C.sub.6)alkyl).sub.2amino-, (C.sub.1-C.sub.6)acylamino-,
cyano, nitro, (C.sub.1-C.sub.6)alkyl-, (C.sub.2-C.sub.6)alkenyl-,
(C.sub.2-C.sub.6)alkynyl-, (C.sub.1-C.sub.6)acylamino-,
cyano(C.sub.1-C.sub.6)alkyl-,
trifluoromethyl(C.sub.1-C.sub.6)alkyl-,
nitro(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C- .sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acylamino-,
amino(C.sub.1-C.sub.6)acyl-,
amino(C.sub.1-C.sub.6)acyl(C.sub.1-C.sub.6)a- lkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)acyl-,
(C.sub.3-C.sub.10)cycloalkyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkoxy(- C.sub.1-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-,
(C.sub.1-C.sub.6)alkyl(difluoromethylene)-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acyl-,
(C.sub.1-C.sub.6)alkylamin- o(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub- .6)acyl-,
(C.sub.6-C.sub.10 )aryl-, (C.sub.1-C.sub.9)heteroaryl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroary- l(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-
(C.sub.3-C.sub.10)cycloalkyl-,
(C.sub.3-C.sub.6)cycloalkyl(C.sub.1-C.sub.- 6)alkyl-,
(C.sub.3-C.sub.10)heterocycloalkyl-, (C.sub.3-C.sub.10)heterocyc-
loalkyl(C.sub.1-C.sub.6)alkyl-, hydroxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(- C.sub.2-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-, and
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)alkyl-; Z is
selected from groups (i) to (iv): 28where R.sup.13 is H, or
(C.sub.1-C.sub.6)alkyl optionally substituted by one or more halo
groups; R.sup.14 is H, (C.sub.1-C.sub.6)alkyl,
trifluoro(C.sub.1-C.sub.6)alkyl-, or phenyl(CH.sub.2)-, wherein
said alkyl and phenyl groups are each optionally substituted by one
or more halo groups, or R.sup.14 is selected from the groups A
above, optionally substituted by one or more halo groups; R.sup.15
is selected from hydroxy, halo, (C.sub.1-C.sub.6)alkyl- optionally
substituted by one or more halo, and (C.sub.1-C.sub.6)alkoxy-
optionally substituted by one or more halo; and J is S, O, --NH--,
or NCH.sub.3; 29where R.sup.16 is H, or (C.sub.1-C.sub.6)alkyl
optionally substituted by one or more, halo groups; R.sup.17 is a
group selected from R.sup.14 above, or R.sup.14K-- where K is
--C(O)-- or --SO.sub.2--; and R.sup.18 is selected from hydroxy,
halo, (C.sub.1-C.sub.6)alkyl- optionally substituted by one or more
halo, and (C.sub.1-C.sub.8)alkoxy- optionally substituted by one or
more halo; and J is S, O, --NH--, or NCH.sub.3; 30where R.sup.19 is
H, or (C.sub.1-C.sub.6)alkyl optionally substituted by one or more
halo groups; R.sup.20 is H, (C.sub.1-C.sub.6)alkyl,
trifluoro(C.sub.1-C.sub.6)- alkyl-; or phenyl(CH.sub.2)-, wherein
said alkyl and phenyl groups are each optionally substituted by one
or more halo groups, or R.sup.20 is selected from the groups A
above, optionally substituted by one or more halo groups; and
R.sup.21 is selected from hydroxy, halo, (C.sub.1-C.sub.6)alkyl-
optionally substituted by one or more halo, and
(C.sub.1-C.sub.8)alkoxy- optionally substituted by one or more
halo; and 31where R.sup.22 is H, or (C.sub.1-C.sub.6)alkyl
optionally substituted by one or more halo groups; R.sup.23 is a
group selected from R.sup.14 above, or is R.sup.14K-- where K is
--C(O)-- or --SO.sub.2--; and R.sup.24 is selected from hydroxy,
halo, (C.sub.1-C.sub.6)alkyl- optionally substituted by one or more
halo, and (C.sub.1-C.sub.8)alkoxy- optionally substituted by one or
more halo; W is (a): 32wherein n is 2-5, R.sup.3 and R.sup.6 are
each independently selected from H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)--, wherein said alkyl and phenyl groups are
optionally substituted by one or more halo groups; R.sup.4 is
selected from H, (C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--,
wherein said alkyl and phenyl groups are optionally substituted by
one or more halo groups;or is 33where groups R.sup.10, R.sup.11 and
R.sup.11, are each, independently, selected from H,
(C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--, wherein said alkyl
and phenyl groups are optionally substituted by one or more halo
groups; R.sup.5 is H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)-, wherein said alkyl and phenyl groups are
optionally substituted by one or more halo groups; or is 34wherein
R.sup.12 and R.sup.12' are each independently selected from H,
(C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--, wherein said alkyl
and phenyl groups are optionally substituted by one or more halo
groups; or W is (b) 35wherein Q is selected from the group
consisting of: (i) (C.sub.6-C.sub.10)aryl-, selected from phenyl or
naphthyl; (ii) (C.sub.1-C.sub.9)heteroaryl-, selected from the
group consisting of furyl-, thienyl-thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl- quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-; (iii)
(C.sub.3-C.sub.10)cycloalkyl that is selected from the group
consisting of cyclopropyl-, cyclobutyl-, cyclopentyl-; cyclohexyl-,
cycloheptyl-, cyclopropenyl-, cyclobutenyl-cyclopentenyl-,
cyclohexenyl-, cycloheptenyl-, 1,3-cyclobutadienyl-,
1,3-cyclopentadienyl-, 1,3-cyclohexadienyl-, 1,4-cyclohexadienyl-
1,3-cycloheptadienyl-, 1,4-cycloheptadienyl-,
1,3,5-cycloheptatrienyl- bicyclo[3.2.1]octane,
bicyclo[2.2.1]heptane and the norborn-2-ene unsaturated form
thereof; and (iv) (C.sub.3-C.sub.10)heterocycloalkyl that is
selected from the group consisting of pyrrolidinyl-,
tetrahydrofuranyl- dihydrofuranyl-, tetrahydropyranyl-, pyranyl-,
thiopyranyl-, aziridinyl-, oxiranyl-, methylenedioxyl-, chromenyl-,
isoxazolidinyl-, 1,3-oxazolidin-3-yl-isothi- azolidinyl-,
1,3-thiazolidin-3-yl-, 1,2-pyrazolidin-2-yl-,
1,3-pyrazolidin-1-yl-, piperidinyl-, thiomorpholinyl-,
1,2-tetrahydrothiazin-2-yl-, 1,3-tetrahydrothiazin-3-yl-,
tetrahydrothiadiazinyl-, morpholinyl-, 1,2-tetrahydrodiazin-2-yl-,
1,3-tetrahydrodiazin-1-yl-, tetrahydroazepinyl-, piperazinyl-, and
chromanyl; and R.sup.7, R.sup.8, and R.sup.9 are each independently
selected from H, (C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--,
wherein said alkyl and phenyl groups are optionally substituted by
one or more halo groups.
2. The compound of claim 1, wherein group A' is a
(C.sub.6-C.sub.10) aryl group selected from phenyl and
naphthyl.
3. The compound of claim 1, wherein group A' is a a
(C.sub.1-C.sub.9) heteroaryl group that is selected from the group
consisting of furyl-, thienyl-, thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl-, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl-, quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-.
4. The compound of claim 1, wherein group A' is optionally
substituted by one to five groups, each independently selected from
the group consisting of hydroxy, halo, amino, trifluoromethyl,
carboxy, (C.sub.1-C.sub.6)alkoxy-, (C.sub.1-C.sub.6)acyloxy-,
(C.sub.1-C.sub.6)alkylamino-, ((C.sub.1-C.sub.6)alkyl).sub.2amino-,
(C.sub.1-C.sub.6)acylamino-, cyano, nitro, (C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkenyl-, (C.sub.2-C.sub.6)alkynyl-,
(C.sub.1-C.sub.6)acylamino-, cyano(C.sub.1-C.sub.6)alkyl-,
trifluoromethyl(C.sub.1-C.sub.6)alkyl-,
nitro(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acylamino-,
amino(C.sub.1-C.sub.6)acyl-,
amino(C.sub.1-C.sub.6)acyl(C.sub.1-C.sub.6)a- lkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)acyl-,
(C.sub.3-C.sub.10)cycloalkyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkoxy(- C.sub.1-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-,
(C.sub.1-C.sub.6)alkyl(difluoromethylene)-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acyl-,
(C.sub.1-C.sub.6)alkylamin- o(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub- .6)acyl-,
(C.sub.6-C.sub.10)aryl-, (C.sub.5-C.sub.9)heteroaryl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.9)heteroary- l(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-(C.sub-
.3-C.sub.6)cycloalkyl-,
(C.sub.3-C.sub.6)cycloalkyl(C.sub.1-C.sub.6)alkyl-- ,
(C.sub.3-C.sub.10)heterocycloalkyl-,
(C.sub.3-C.sub.10)heterocycloalkyl(- C.sub.1-C.sub.6)alkyl-,
hydroxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(- C.sub.2-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-, and
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)alkyl.
5. The compound of claim 1, wherein group Q of group W, option (a),
is a (C.sub.6-C.sub.10) aryl group selected from phenyl and
naphthyl.
6. The compound of claim 1, wherein group Q of group W, option (a),
is a (C.sub.1-C.sub.9) heteroaryl group that is selected from the
group consisting of furyl-, thienyl-thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl-, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl-, quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-.
7. The compound of claim 1, wherein group Q of group W, option (a),
is a (C.sub.3-C.sub.10)cycloalkyl group that is selected from the
group consisting of cyclopropyl-cyclobutyl-, cyclopentyl-,
cyclohexyl-, cycloheptyl-cyclopropenyl-, cyclobutenyl-,
cyclopentenyl-, cyclohexenyl-, cycloheptenyl-,
1,3-cyclobutadienyl-, 1,3-cyclopentadienyl-, 1,3-cyclohexadienyl-,
1,4-cyclohexadienyl-, 1,3-cycloheptadienyl-, 1,4-cycloheptadienyl-,
1,3,5-cycloheptatrienyl-, bicyclo[3.2.1]octane-,
bicyclo[2.2.1]heptane-, and the norborn-2-ene unsaturated form
thereof.
8. The compound of claim 1, wherein group Q of group W, option (a),
is a (C.sub.3-C.sub.10)heterocycloalkyl group that is selected from
the group consisting of pyrrolidinyl-,
tetrahydrofuranyl-dihydrofuranyl-, tetrahydropyranyl-, pyranyl-,
thiopyranyl-, aziridinyl-, oxiranyl-, methylenedioxyl-, chromenyl-,
isoxazolidinyl-, 1,3-oxazolidin-3-yl-isothi- azolidinyl-,
1,3-thiazolidin-3-yl-, 1,2-pyrazolidin-2-yl-,
1,3-pyrazolidin-1-yl-, piperidinyl-, thiomorpholinyl-,
1,2-tetrahydrothiazin-2-yl-, 1,3-tetrahydrothiazin-3-yl-,
tetrahydrothiadiazinyl-, morpholinyl-, 1,2-tetrahydrodiazin-2-yl-,
1,3-tetrahydrodiazin-1-yl-, tetrahydroazepinyl-, piperazinyl-, and
chromanyl.
9. The compound of claim 1 wherein component W thereof is an
optionally substituted histidine residue.
10. The compound of claim 1, wherein one or more of R.sup.13,
R.sup.16, R.sup.19 and R.sup.22 is trifluoromethyl.
11. The compound of claim 1 wherein an R group selected from the
group consisting of R.sup.15, R.sup.18, R.sup.21 and R.sup.24 is
trifluoromethyl.
12. The compound of claim 1 wherein an R group selected from the
group consisting of R.sup.14, R.sup.17, R.sup.20, and R.sup.23 is
trifluoromethyl.
13. A compound selected from the group consisting of
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl]-amino}-hexanoic acid methyl ester;
6-Amino-2-{[2-(biphenyl-4--
sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexano-
ic acid methyl ester;
6-Amino-2-{[2-(biphenyl-4-carbonyl)-2,3,4,9-tetrahyd-
ro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic acid methyl
ester;
6-Amino-2-[(2-biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl)-amino]-hexanoic acid methyl ester;
6-Amino-2-{[1-(3-benzyloxy--
phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic
acid methyl ester;
6-Amino-2-({1-[4-(4-trifluoromethyl-phenoxy)-phenyl]-2-
,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic
acid methyl ester;
6-Amino-2-{[1-(4-butyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-
-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
6-Amino-2-{[1-(4-pyrrolidin-1-yl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester;
6-Amino-2-({1-[2-(4-isopropyl-phenyl)-1-methyl-ethyl]-2,3,4,9-tetrahydro--
1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl ester;
6-Amino-2-({1-[3-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1-
H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl ester;
6-Amino-2-{[1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl]-amino}-hexanoic acid methyl ester;
6-Amino-2-{[1-(3-benzyloxy-
-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoi-
c acid methyl ester;
6-Amino-2-{[1-(1-methyl-3-phenyl-butyl)-2,3,4,9-tetra-
hydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic acid methyl
ester;
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
5-Guanidino-2-{[2-(toluene-4-sulfonyl)-1,2,3,4-tetrahydro-isoquinoline-3--
carbonyl]-amino}-pentanoic acid tert-butyl ester;
5-Guanidino-2-{[2-(tolue-
ne-4-sulfonyl)-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl]-amino}-pentanoi-
c acid methyl ester;
6-Amino-2-({2-[4-(2-oxo-2,3-dihydro-benzoimidazol-1-y-
l)-piperidine-1-carbonyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbony-
l}-amino)-hexanoic acid tert-butyl ester;
6-Amino-2-({2-[(1H-indol-3-yl)-a-
cetyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic
acid methyl ester;
6-Amino-2-{[1-(4-isopropyl-phenyl)-2,3,4,9-tetrahydro--
1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
and
6-Amino-2-{[1-(3-trifluoromethyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester.
14. A compound selected from the group consisting of
6-Amino-2-{[4-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.--
carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
6-Amino-2-{[4-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.--
carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-{[2-(biphenyl-4-sulfonyl
)-2,3,4,9-tetrahydro-1H-.beta.-carboli-
ne-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-{[2-(biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-carbolin-
e-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-[(2-biphenyl-4-yl
methyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl)-amino]-hexanoic acid ter-butyl ester;
2-(Toluene-4-sulfonyl)--
2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxylic acid
(3-aminomethyl-cyclohexylmethyl)-amide;
2-(Toluene-4-sulfonyl)-2,3,4,9-te-
trahydro-1H-.beta.-carboline-3-carboxylic acid
(4-aminomethyl-pyridin-2-yl- methyl)-amide;
2-(Biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carbol-
ine-3-carboxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
2-(Biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxyl-
ic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
2-(Biphenyl-4-carbonyl)--
2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxylic acid
(3-aminomethyl-cyclohexylmethyl)-amide;
2-(Biphenyl-4-carbonyl)-2,3,4,9-t-
etrahydro-1H-.beta.-carboline-3-carboxylic acid
(4-aminomethyl-pyridin-2-y- lmethyl)-amide;
2-Biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carboli-
ne-3-carboxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
2-Biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxylic
acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
6-Amino-2-{[1-(3-benzyloxy-
-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoi-
c acid tert-butyl ester;
1-(3-Benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carboxylic acid
(3-aminomethyl-cyclohexylmethyl)-amide;
1-(3-Benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxyli-
c acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
1-[4-(4-Trifluoromethyl-p-
henoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxylic
acid (3-aminomethyl-cyclohexylmethyl)-amide;
1-[4-(4-Trifluoromethyl-phenoxy)--
phenyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carboxylic acid
(4-aminomethyl-pyridin-2-ylmethyl)-amide;
6-Amino-2-({1-[4-(4-trifluorome-
thyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl}-a-
mino)-hexanoic acid tert-butyl ester;
6-Amino-2-{[2-(4-methyl-benzoyl)-2,3-
,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic
acid tert-butyl ester;
6-Amino-2-{[2-(4-methyl-benzyl)-2,3,4,9-tetrahydro-1H-.-
beta.-carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-[(2-benzyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl)-a-
mino]-hexanoic acid tert-butyl ester;
6-Amino-2-[(2-benzoyl-2,3,4,9-tetrah-
ydro-1H-.beta.-carboline-3-carbonyl)-amino]-hexanoic acid
tert-butyl ester;
6-Amino-2-[(2-benzenesulfonyl-2,3,4,9-tetrahydro-1H-.beta.-carboli-
ne-3-carbonyl)-amino]-hexanoic acid tert-butyl ester;
6-Amino-2-{[1-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.--
carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-{[1(3-benzyloxy-phenyl)-1-methyl-2,3,4,9-tetrahydro-1H-.beta.-c-
arboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-({1-[3-(4-fluoro-benzyloxy)-phenyl]-2,3,4,9-tetrahydro-1H-.beta-
.-carboline-3-carbonyl}-amino)-hexanoic acid tert-butyl ester;
6-Amino-2-{[1-(2-benzyloxy-pyridin-4-yl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
6-Amino-2-({1-[3-(pyridin-2-ylmethoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carbonyl}-amino)-hexanoic acid tert-butyl ester;
6-Amino-2-({1-[3-(1-phenyl-ethoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.beta.-c-
arboline-3-carbonyl}-amino)-hexanoic acid tert-butyl ester;
6-Amino-2-{[1-(4-phenoxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-
-carbonyl]-amino}-hexanoic acid methyl ester;
2-[(2-Benzoyl-1,2,3,4-tetrah-
ydro-isoquinoline-3-carbonyl)-amino]-5-guanidino-pentanoic acid
tert-butyl ester;
2-[(2-Benzyl-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl)-amino]-5--
guanidino-pentanoic acid tert-butyl ester; and
2-[(2-Benzenesulfonyl-1,2,3-
,4-tetrahydro-isoquinoline-3-carbonyl)-amino]-5-guanidino-pentanoic
acid tert-butyl ester.
15. A pharmaceutical composition for increasing growth hormone
secretion in a mammal, comprising an effective amount of a compound
according to claim 1, and a pharmaceutical carrier.
16. A pharmaceutical composition for increasing secretion of
gastrin or glucagon in a mammal, comprising an effective amount of
a compound according to claim 1, and a pharmaceutical carrier.
17. A pharmaceutical composition for inhibiting the binding of
somatostatin to an sst2 receptor, comprising an effective amount of
a compound according to claim 1, and a pharmaceutical carrier.
18. A method for increasing growth hormone secretion in a mammal,
comprising administering an effective amount of a pharmaceutical
composition according to claim 15.
19. A method for increasing secretion of gastrin or glucagon in a
mammal, comprising administering an effective amount of a
pharmaceutical composition according to claim 16.
20. A method for decreasing somatostatin-induced downregulation of
growth hormone secretion in a mammal, comprising administering an
effective amount of a pharmaceutical composition according to claim
17.
21. A pharmaceutical composition useful to cause sustained release
of growth hormone in a mammal in need thereof, comprising a
compound according to claim 1, and a pharmaceutical carrier.
22. A method for facilitating the sustained secretion of growth
hormone in a mammal in need thereof, wherein said mammal possesses:
(a) a defect in (1) the expression of the encoding nucleotide
sequence for growth hormone, (2) the processing of resultant mRNA,
or (3) the translation or intracellular processing and packaging of
GH or precursor polypeptide thereof; or (b) an allele of the growth
hormone gene which codes for a growth hormone polypeptide that is
insufficiently active; which comprises administering an effective
amount of a pharmaceutical composition according to claim 21.
23. A method for treating a human for one or more symptoms of
insufficient growth hormone secretion, wherein said symptom is
selected from frailty, hypoglycemia, wrinkled skin, slow skeletal
growth, reduced immune function, and reduced organ function,
comprising administering an effective amount of a pharmaceutical
composition according to claim 15.
24. A method for treating a non-human mammal to enhance the growth
and performance thereof, comprising administering an effective
amount of a pharmaceutical composition according to claim 15.
25. A pharmaceutical composition according to claim 15 further
comprising growth hormone releasing peptide (GHRP) or growth
hormone releasing hormone (GHRH).
26. A method for increasing growth hormone secretion in a mammal,
comprising administering an effective amount of a pharmaceutical
composition according to claim 25.
27. A method for increasing growth hormone secretion in a mammal,
comprising administering an effective amount of a pharmaceutical
composition according to claim 15, and a further composition
comprising growth hormone releasing peptide (GHRP) or growth
hormone releasing hormone (GHRH).
Description
FIELD OF THE INVENTION
[0001] The present invention provides pharmaceutically active
compounds that facilitate secretion of growth hormone (GH) by the
anterior pituitary. Growth hormone (also known as somatotropin)
acts indirectly to promote skeletal growth in children by
stimulating the production of insulin like growth factor-1 from the
liver. Growth hormone also stimulates the differentiation of fat
cells and chondrocytes (cells that secrete collagen and
proteoglycans to form cartilage). In adults, growth hormone is
involved in the proper maintenance of connective and muscle
tissues.
[0002] Growth hormone deficiency may be congenital or acquired.
Deficiency in children causes slow skeletal growth that, if not
corrected, results in permanent short stature. In older adults,
deficiency of growth hormone results in frailty. Additional adult
symptoms of GH deficiency may include wrinkled skin and
hypoglycemia.
[0003] For veterinary application, upregulation of growth hormone
is useful to treat frailty in older animals, particularly companion
animals. With respect to livestock, upregulation of growth hormone
increases growth and performance, even in healthy animals with
normal GH levels. Improvements in feed efficiency, milk yield,
leanness, meat quality and fertility are of note.
[0004] Although direct administration of growth hormone may be
effective in certain therapeutic applications, it is difficult in
practice. Among other issues, since the half-life of growth hormone
in the body is very short, direct administration leads to
artificially increased levels in the concentration of circulating
GH, which then rapidly drop off. Sustained release, such as by a
mechanical pump, has not been optimally set to practice.
[0005] The concentration of growth hormone circulating in the body
depends on the balance of numerous biochemical pathways, including
opposing processes. Compared to the direct administration approach,
shifting the balance of these pathways indirectly provides a safer,
more reproducible method to affect GH secretion on a sustained
basis. Under this approach, since the overall regulatory framework
remains intact, secretion rates and circulatory concentrations for
GH follow a relatively normal pattern, and adverse fluctuations in
both secretion rate and circulating GH concentration are avoided.
The present invention provides for therapeutic compounds, and their
use, to indirectly elevate growth hormone secretion from the
pituitary.
Reported Developments
[0006] Growth hormone is released from the anterior pituitary in
response to stimulation by growth hormone releasing peptide (GHRP),
and growth hormone releasing hormone (GHRH), of hypothalamic
origin. However, release of growth hormone via these or other
mechanisms is inhibited by somatostatin, and thus the process is
closely regulated.
[0007] Somatostatin (SRIF) is a cyclic peptide hormone of 14 amino
acids (there is also a 28 amino acid form) having numerous
endocrine functions which, like many hormones, is cleaved from a
larger precursor protein. Somatostatin inhibits the pituitary
secretion of growth hormone, the pancreatic secretion of glucagon
and insulin, and the secretion of gastrin from the gut.
Somatostatin also acts as a neurotransmitter/neurom- odulator (see.
S. J. Hocart et al., J. Med. Chem.,41, pp. 1146-1154, 1998 for a
general discussion).
[0008] The biological effects of somatostatin are apparently all
inhibitory in nature, and are elicited upon binding to the surface
of a target cell. The receptor is an integral membrane protein
(which spans the cell membrane), and is G-protein-coupled.
G-protein coupled receptors represent a major class of cell surface
receptors. It is believed that upon binding of somatostatin to the
receptor, the receptor undergoes a conformational change
facilitating its interaction with a G-protein at the cytoplasmic
face of the receptor. This facilitates binding or release of.
GTP/GDP at the G protein, and leads to further activation and
signaling events inside the cell. In particular, somatostatin
binding at its own G-protein-coupled receptor is negatively coupled
to adenylyl cyclase activity, which is necessary for the
production, of cyclic AMP. Thus, these further signaling events
directly oppose mechanisms (for example, as mediated by calcium
ions or cyclic AMP) whereby GHRP and GHRH would otherwise trigger.
extracellular secretion of growth hormone from cytoplasmic storage
granules. For a general review thereof, see The Encyclopedia of
Molecular Biology, J. Kendrew, ed., Blackwell Science, Ltd. 1994,
at page 387.
[0009] The effects of somatostatin on target cells are mediated by
at least 5 classes of receptors (sst1-sst5). Although the receptors
may have similar affinity for somatostatin, they are differentially
expressed in different tissues, and so positioned, interact,
directly or indirectly, with different intracellular signaling
components. This tissue specificity of receptor expression accounts
in large measure for the different effects of somatostatin in
different target cell types. Somatostatin receptors are found, for
example, in tissues of the anterior pituitary, other brain tissues,
the pancreas, the lung, on lymphocytes, and on mucosa cells of the
intestinal tract.
[0010] The sst2 type receptor is known to mediate inhibition of
growth hormone secretion in the anterior pituitary. This receptor
is also reported in 2 forms, proteins sst2A and sst2B, which result
from differential splicing of the sst2 gene transcript (M. Vanetti,
et al., FEBS Letters, 311, pp.290-294, 1992). The sst2 receptor is
also known to mediate inhibition of gastrin and histamine
secretion. Additionally, the sst2 receptor is known to mediate
inhibition of glucagon release from pancreatic alpha cells.
[0011] Although numerous somatostatin agonists have been described
(see for example, WO 98/44922, WO 98/45285, and WO 98/44921), the
development of useful sst2-linked somatostatin antagonists has
lagged behind. Recent reports of such compounds include W. R.
Baumbach et al., Molecular Pharmacology, 54, pp. 864-873, 1998, and
S. J. Hocart et al., J. Med. Chem., 41, pp. 1146-1154, 1998.
However, such compounds are short peptides, a class of molecules
not often suited for successful use as pharmaceuticals because of
their typically short half life in the body. Additional relevant
disclosures include WO99/64401 and WO99/64420.
[0012] It would be advantageous to provide antagonists of
somatostatin activity, effective at the sst2 type receptor, having
superior properties as pharmaceuticals, including bioavailability,
stability, and the like. The present invention provides a series of
antagonist compounds that specifically interfere with the binding
of somatostatin to the sst subtype 2 receptors of cells in the
mammalian anterior pituitary, and which have additional valuable
properties.
SUMMARY OF THE INVENTION
[0013] Accordingly, there are provided compounds according to the
formula
A-Z-W (formula I)
[0014] or pharmaceutically acceptable salts, solvates or hydrates
thereof; wherein A is selected from the groups consisting of:
[0015] A'-(CH.sub.2).sub.n--, A'-(CH.sub.2).sub.nSO.sub.2--, and
A'-(CH.sub.2).sub.nCO--, where n is 0 to 4; and
[0016] A' is selected from
[0017] (a) (C.sub.6-C.sub.10)aryl-, selected from phenyl or
naphthyl; or
[0018] (b) (C.sub.1-C.sub.9)heteroaryl-, selected from the group
consisting of furyl-, thienyl-, thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl- quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-;
[0019] wherein said A' group (a) or (b) is optionally substituted
by zero to seven, preferably zero to five groups, each
independently selected from:
[0020] hydroxy, halo, amino, trifluoromethyl-, carboxy,
(C.sub.1-C.sub.6)alkoxy-, (C.sub.1-C.sub.6)acyloxy-,
(C.sub.1-C.sub.6)alkylamino-, ((C.sub.1-C.sub.6)alkyl).sub.2amino-,
(C.sub.1-C.sub.6)acylamino-, cyano, nitro, (C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkenyl-, (C.sub.2-C.sub.6)alkynyl-,
(C.sub.1-C.sub.6)acylamino-, cyano(C.sub.1-C.sub.6)alkyl-,
trifluoromethyl(C.sub.1-C.sub.6)alkyl-,
nitro(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acylamino-,
amino(C.sub.1-C.sub.6)acyl-,
amino(C.sub.1-C.sub.6)acyl(C.sub.1-C.sub.6)a- lkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)acyl-,
(C.sub.3-C.sub.10)cycloalkyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkoxy(- C.sub.1-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-,
(C.sub.1-C.sub.6)alkyl(difluoromethylene)-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acyl-,
(C.sub.1-C.sub.6)alkylamin- o(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub- .6)acyl-,
(C.sub.6-C.sub.10)aryl-, (C.sub.1-C.sub.9)heteroaryl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroary- l(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-
(C.sub.3-C.sub.6)cycloalkyl-,
(C.sub.3-C.sub.6)cycloalkyl(C.sub.1-C.sub.6- )alkyl-,
(C.sub.3-C.sub.10)heterocycloalkyl-, (C.sub.3-C.sub.10)heterocycl-
oalkyl(C.sub.1-C.sub.6)alkyl-, hydroxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(- C.sub.2-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-, and
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)alkyl-;
[0021] Z is selected from groups (i) to (iv): 1
[0022] where R.sup.13 is H, or (C.sub.1-C.sub.6)alkyl optionally
substituted by one or more halo groups;
[0023] R.sup.14 is H, (C.sub.1-C.sub.6)alkyl,
trifluoro(C.sub.1-C.sub.6)al- kyl-, or phenyl(CH.sub.2)--, wherein
said alkyl and phenyl groups are each optionally substituted by one
or more halo groups, or R.sup.14 is selected from the groups A
above, optionally substituted by one or more halo groups;
[0024] R.sup.15 is selected from hydroxy, halo,
(C.sub.1-C.sub.6)alkyl- optionally substituted by one or more halo,
and (C.sub.1-C.sub.6)alkoxy- optionally substituted by one or more
halo; and
[0025] J is S, O, --NH--, or NCH.sub.3; 2
[0026] where R.sup.16 is H, or (C.sub.1-C.sub.6)alkyl optionally
substituted by one or more halo groups;
[0027] R.sup.17 is a group selected from R.sup.14 above, or
R.sup.14K-- where K is --C(O)-- or --SO.sub.2--; and
[0028] R.sup.18 is selected from hydroxy, halo,
(C.sub.1-C.sub.6)alkyl- optionally substituted by one or more halo,
and (C.sub.1-C.sub.8)alkoxy- optionally substituted by one or more
halo; and
[0029] J is S, O, --NH--, or NCH.sub.3; 3
[0030] where R.sup.19 is H, or (C.sub.1-C.sub.6)alkyl optionally
substituted by one or more halo groups;
[0031] R.sup.20 is H, (C.sub.1-C.sub.6)alkyl,
trifluoro(C.sub.1-C.sub.6)al- kyl-; or phenyl(CH.sub.2)--, wherein
said alkyl and phenyl groups are each optionally substituted by one
or more halo groups, or R.sup.20 is selected from the groups A
above, optionally substituted by one or more halo groups; and
[0032] R.sup.21 is selected from hydroxy, halo,
(C.sub.1-C.sub.6)alkyl- optionally substituted by one or more halo,
and (C.sub.1-C.sub.8)alkoxy- optionally substituted by one or more
halo; and 4
[0033] where R.sup.22 is H, or (C.sub.1-C.sub.6)alkyl optionally
substituted by one or more halo groups;
[0034] R.sup.23 is a group selected from R.sup.14 above, or is
R.sup.14K-- where K is --C(O)-- or --SO.sub.2--; and
[0035] R.sup.24 is selected from hydroxy, halo,
(C.sub.1-C.sub.6)alkyl- optionally substituted by one or more halo,
and (C.sub.1-C.sub.8)alkoxy- optionally substituted by one or more
halo;
[0036] W is (a): 5
[0037] wherein n is 2-5,
[0038] R.sup.3 and R.sup.6 are each independently selected from H,
(C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--, wherein said alkyl
and phenyl groups are optionally substituted by one or more halo
groups;
[0039] R.sup.4 is selected from H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)--, wherein said alkyl and phenyl groups are
optionally substituted by one or more halo groups;or is 6
[0040] where groups R.sup.10, R.sup.11 and R.sup.11, are each,
independently, selected from H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)--, wherein said alkyl and phenyl groups are
optionally substituted by one or more halo groups;
[0041] R.sup.5 is H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)--, wherein said alkyl and phenyl groups are
optionally substituted by one or more halo groups; or is 7
[0042] wherein R.sup.12 and R.sup.12' are each independently
selected from H, (C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--,
wherein said alkyl and phenyl groups are optionally substituted by
one or more halo groups;
[0043] or W is (b) 8
[0044] wherein Q is selected from the group consisting of:
[0045] (i) (C.sub.6-C.sub.10)aryl-, selected from phenyl or
naphthyl;
[0046] (ii) (C.sub.1-C.sub.9)heteroaryl-, selected from the group
consisting of furyl-, thienyl-, thiazolyl-, pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-, 1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl- quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, and benzoxazinyl-;
[0047] (iii) (C.sub.3-C.sub.10)cycloalkyl that is selected from the
group consisting of cyclopropyl-, cyclobutyl-, cyclopentyl-;
cyclohexyl-, cycloheptyl-, cyclopropenyl-, cyclobutenyl-,
cyclopentenyl-, cyclohexenyl-, cycloheptenyl-,
1,3-cyclobutadienyl-, 1,3-cyclopentadienyl-, 1,3-cyclohexadienyl-,
1,4-cyclohexadienyl-1,3-cycl- oheptadienyl-, 1,4-cycloheptadienyl-,
1,3,5-cycloheptatrienyl- bicyclo[3.2.1]octane, bicyclo [2.2.1]
heptane and the norborn-2-ene unsaturated form thereof; and
[0048] (iv) (C.sub.3-C.sub.10)heterocycloalkyl that is selected
from the group consisting of pyrrolidinyl-, tetrahydrofuranyl-
dihydrofuranyl-, tetrahydropyranyl-, pyranyl-, thiopyranyl-,
aziridinyl-, oxiranyl-, methylenedioxyl-, chromenyl-,
isoxazolidinyl-, 1,3-oxazolidin-3-yl-isothi- azolidinyl-,
1,3-thiazolidin-3-yl-, 1,2-pyrazolidin-2-yl-,
1,3-pyrazolidin-1-yl-, piperidinyl-, thiomorpholinyl-,
1,2-tetrahydrothiazin-2-yl-, 1,3-tetrahydrothiazin-3-yl-,
tetrahydrothiadiazinyl-, morpholinyl-, 1,2-tetrahydrodiazin-2-yl-,
1,3-tetrahydrodiazin-1 -yl-, tetrahydroazepinyl-, piperazinyl-, and
chromanyl;
[0049] and R.sup.7, R.sup.8, and R.sup.9 are each independently
selected from H, (C.sub.1-C.sub.8)alkyl-, and phenyl(CH.sub.2)--,
wherein said alkyl and phenyl groups are optionally substituted by
one or more halo groups.
[0050] In a preferred embodiment of the invention, where one or
more of R.sup.13, R.sup.16, R.sup.19 and R.sup.22 is
halo(C.sub.1-C.sub.6)alkyl-, the preferred halo species is fluoro,
wherein trifluoromethyl- is most preferred.
[0051] Preferred compounds of the invention include:
[0052] 6-Amino-2-{[2-(toluene-4-sulfonyl
)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0053]
6-Amino-2-{[2-(biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0054]
6-Amino-2-{[2-(biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0055]
6-Amino-2-[(2-biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carb-
oline-3-carbonyl)-amino]-hexanoic acid methyl ester;
[0056]
6-Amino-2-{[1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0057]
6-Amino-2-({1-[4-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrah-
ydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl
ester;
[0058]
6-Amino-2-{[1-(4-butyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboli-
ne-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0059]
6-Amino-2-{[1-(4-pyrrolidin-1-yl-phenyl)-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0060]
6-Amino-2-({1-[2-(4-isopropyl-phenyl)-1-methyl-ethyl]-2,3,4,9-tetra-
hydro-1-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl
ester;
[0061]
6-Amino-2-({1-[3-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrah-
ydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl
ester;
[0062]
6-Amino-2-([1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0063]
6-Amino-2-{[1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0064]
6-Amino-2-{[1-(1-methyl-3-phenyl-butyl)-2,3,4,9-tetrahydro-1H-.beta-
.-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0065]
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0066]
5-Guanidino-2-{[2-(toluene-4-sulfonyl)-1,2,3,4-tetrahydro-isoquinol-
ine-3-carbonyl]-amino}-pentanoic acid tert-butyl ester;
[0067]
5-Guanidino-2-{[2-(toluene-4-sulfonyl)-1,2,3,4tetrahydro-isoquinoli-
ne-3-carbonyl]-amino}-pentanoic acid methyl ester;
[0068]
6-Amino-2-({2-[4-(2-oxo-2,3-dihydro-benzoimidazol-1-yl)-piperidine--
1-carbonyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexa-
noic acid tert-butyl ester;
[0069]
6-Amino-2-({2-[(1H-indol-3-yl)-acetyl]-2,3,4,9-tetrahydro-1H-.beta.-
-carboline-3-carbonyl}-amino)-hexanoic acid methyl ester;
[0070]
6-Amino-2-{[1-(4-isopropyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid methyl ester; and
[0071]
6-Amino-2-{[1-(3-trifluoromethyl-phenyl)-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester.
[0072] Additional compounds of the invention include:
[0073]
6-Amino-2-{[4-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.-
beta.-carboline-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0074]
6-Amino-2-{[4-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.-
beta.-carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl
ester;
[0075]
6-Amino-2-{[2-(biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0076]
6-Amino-2-{[2-(biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0077]
6-Amino-2-[(2-biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carb-
oline-3-carbonyl)-amino]-hexanoic acid tert-butyl ester;
[0078]
2-(Toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-car-
boxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
[0079]
2-(Toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-car-
boxylic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0080]
2-(Biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-ca-
rboxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
[0081]
2-(Biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-ca-
rboxylic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0082]
2-(Biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-ca-
rboxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
[0083]
2-(Biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-ca-
rboxylic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0084]
2-Biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carb-
oxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
[0085]
2-Biphenyl-4-ylmethyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carb-
oxylic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0086]
6-Amino-2-{[1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0087]
1-(3-Benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-car-
boxylic acid (3-aminomethyl-cyclohexylmethyl)-amide;
[0088]
1-(3-Benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-car-
boxylic acid (4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0089]
1-[4-(4-Trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carboxylic acid
(3-aminomethyl-cyclohexylmethyl)-amide;
[0090]
1-[4-(4-Trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carboxylic acid
(4-aminomethyl-pyridin-2-ylmethyl)-amide;
[0091]
6-Amino-2-({1-[4-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrah-
ydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid
tert-butyl ester;
[0092]
6-Amino-2-{[2-(4-methyl-benzoyl)-2,3,4,9-tetrahydro-1H-.beta.-carbo-
line-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0093]
6-Amino-2-{[2-(4-methyl-benzyl)-2,3,4,9-tetrahydro-1H-.beta.-carbol-
ine-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0094]
6-Amino-2-[(2-benzyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbo-
nyl)-amino]-hexanoic acid tert-butyl ester;
[0095]
6-Amino-2-[(2-benzoyl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carb-
onyl)-amino]-hexanoic acid tert-butyl ester;
[0096]
6-Amino-2-[(2-benzenesulfonyl-2,3,4,9-tetrahydro-1H-.beta.-carbolin-
e-3-carbonyl)-amino]-hexanoic acid tert-butyl ester;
[0097]
6-Amino-2-{[1-methyl-2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.-
beta.-carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl
ester;
[0098]
6-Amino-2-{[1-(3-benzyloxy-phenyl)-1-methyl-2,3,4,9-tetrahydro-1H-.-
beta.-carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl
ester;
[0099]
6-Amino-2-({1-[3-(4-fluoro-benzyloxy)-phenyl]-2,3,4,9-tetrahydro-1H-
-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid tert-butyl
ester;
[0100]
6-Amino-2-{[1-(2-benzyloxy-pyridin-4-yl)-2,3,4,9-tetrahydro-1H-.bet-
a.-carboline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester;
[0101]
6-Amino-2-({1-[3-(pyridin-2-ylmethoxy)-phenyl]-2,3,4,9-tetrahydro-1-
H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid tert-butyl
ester;
[0102]
6-Amino-2-({1-[3-(1-phenyl-ethoxy)-phenyl]-2,3,4,9-tetrahydro-1H-.b-
eta.-carboline-3-carbonyl}-amino)-hexanoic acid tert-butyl
ester;
[0103]
6-Amino-2-{[1-(4-phenoxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carbo-
line-3-carbonyl]-amino}-hexanoic acid methyl ester;
[0104]
2-[(2-Benzoyl-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl)-amino]-5--
guanidino-pentanoic acid tert-butyl ester;
[0105]
2-[(2-Benzyl-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl)-amino]-5-g-
uanidino-pentanoic acid tert-butyl ester; and
[0106]
2-[(2-Benzenesulfonyl-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl)-a-
mino]-5-guanidino-pentanoic acid tert-butyl ester.
[0107] The compounds and pharmaceutical compositions of this
invention include all conformational isomers of compounds of
formula I (e.g., cis and trans isomers, whether or not involving
double bonds). The compounds of the invention include all optical
isomers of the compounds of formula I (e.g., enantiomers and
diastereomers), as well as racemic, diastereomeric and other
mixtures of all such isomers. This invention further relates to
tautomers and stereoisomers of the compounds of formula (I), and
mixtures of any of the aforementioned forms. As will be described
below in greater detail, certain isomeric structures are
preferred.
[0108] The present invention also relates to the pharmaceutically
acceptable acid addition salts of compounds of the formula (I). The
acids which are used to prepare the pharmaceutically acceptable
acid addition salts of the aforementioned base compounds of this
invention are those which form non-toxic acid addition salts, i.e.
salts containing pharmacologically acceptable anions, such as the
hydrochloride, hydrobromide, hydroiodide, nitrate, sulfate,
bisulfate, phosphate, acid phosphate, acetate, lactate, citrate,
acid citrate, tartrate, bitartrate, succinate, maleate, fumarate,
gluconate, saccharate, benzoate, methanesulfonate, ethanesulfonate,
benzenesulfonate, p-toluenesulfonate and pamoate [i.e.,
1,1'-methylene-bis-(2-hydroxy-3- naphthoate)]salts.
[0109] With respect to the relatively limited number of compounds
that so permit, the invention also relates to base addition salts
of formula (I). The chemical bases that may be used as reagents to
prepare pharmaceutically acceptable base salts of those compounds
of formula I that are acidic in nature are those that form
non-toxic base salts with such compounds. Such non-toxic base salts
include, but are not limited to those derived from such
pharmacologically acceptable cations such as alkali metal cations
(e.g., potassium and sodium) and alkaline earth metal cations (e q,
calcium and magnesium), ammonium or water-soluble amine addition
salts such as N-methylglucamine-(meglumine), and the lower
alkanolammonium and other base salts of pharmaceutically acceptable
organic amines.
[0110] The subject invention also includes isotopically-labelled
compounds, which are identical to those recited in Formula (I), but
for the fact that one or more atoms are replaced by an atom having
an atomic mass or mass number different from the atomic mass or
mass number usually found in nature. Examples of isotopes that can
be incorporated into compounds of the invention include isotopes of
hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and
chlorine, such as .sup.2H, .sup.3H, .sup.13C, .sup.14C, .sup.15N,
.sup.18O, .sup.17O, .sup.31P, .sup.32P, .sup.35S, .sup.18F, and
.sup.36Cl, respectively. Compounds of the present invention,
prodrugs thereof, and pharmaceutically acceptable salts of said
compounds or of said prodrugs which contain the aforementioned
isotopes and/or other isotopes of other atoms are within the scope
of this invention. Certain isotopically-labelled compounds of the
present invention, for example those into which radioactive
isotopes such as .sup.3H and .sup.14C are incorporated, are useful
in drug and/or substrate tissue distribution assays. Tritiated,
i.e., .sup.3H, and carbon-14, i.e., .sup.14C, isotopes are
particularly preferred for their ease of preparation and
detectability. Further, substitution with heavier isotopes such as
deuterium, i.e., .sup.2H, can afford certain therapeutic advantages
resulting from greater metabolic stability, for example increased
in vivo half-life or reduced dosage requirements and, hence, may be
preferred in some circumstances. Isotopically labelled compounds of
Formula (I) of this invention and prodrugs thereof can generally be
prepared by carrying out the procedures disclosed in the Schemes
and/or in the Examples and Preparations below, by substituting a
readily available isotopically labelled reagent for a
non-isotopically labelled reagent.
[0111] The present invention also relates to a pharmaceutical
composition for increasing growth hormone secretion in a mammal,
including a human, comprising an effective amount of a compound
according to formula 1, and a pharmaceutical carrier. The present
invention also relates to a pharmaceutical composition for
increasing gastrin secretion or glucagon secretion in a mammal,
comprising an effective amount of a compound according to formula
1, and a pharmaceutical carrier.
[0112] The present invention also relates to a pharmaceutical
composition for the treatment of diseases characterized by
decreased levels of growth hormone, glucagon, or gastrin in a
mammal, including a human, comprising an amount of a compound of
formula (I) effective in such treatments and a pharmaceutically
acceptable carrier. The present invention also relates to a
pharmaceutical composition for the treatment of diseases in a
mammal, including a human, wherein treatment can be effected by
inhibiting the binding of somatostatin to the sst2-type receptor
therefor, comprising an effective amount of a compound according to
formula 1, and a pharmaceutical carrier.
[0113] The present invention relates to a method for treating
growth hormone deficiency in a mammal, including a human. The
present invention also relates to elevating the level of growth
hormone in a mammal, including a human, wherein this is beneficial
to the mammal nothwithstanding that the natural levels of growth
hormone present in the mammal are within the normal range. In the
practice of said method, there is administered a pharmaceutical
composition of the invention comprising a compound according to
formula (1), and a pharmceutical carrier.
[0114] Similarly, the methods of the invention provide for
increasing gastrin secretion or glucagon secretion in a mammal,
including a human, where this is medically appropriate. For
example, gastrin is involved in protection of gastric mucosa
against damage by chemical substances, e.g. alcohol (S. J. Konturek
et al., European Journal of Pharmacology, 278(3), pp. 203-212,
1995). Glucagon is a counter-regulatory hormone that is used to
treat hypoglycemia, and causes positive inotropic and chronotropic
effects without the need for beta-1 adrenoceptor stimulation. It
also can be used to correct beta-blocker, verapamil and imipramine
overdose, and is used as adjunctive therapy in shock situations,
for heart failure, and in treating postcountershock asystole (see
C. M. White, Journal of Clinical Pharmacology, 39(5), pp. 442-447,
1999).
[0115] In preferred examples of the invention, there are provided
methods for treating a human for one or more symptoms of
insufficient growth hormone secretion, or one or more conditions
that may occur therewith and be exacerbated thereby, wherein said
condition is selected from frailty, hypoglycemia, wrinkled skin,
slow skeletal growth, reduced immune function, reduced organ
function, fertility disorders; bone disease, AIDS-related complex,
cachexia, cardiac failure, ischemic heart disease, colon disease,
metabolic disorders, renal failure, muscular dystrophy, and Turners
syndrome, comprising administering an effective amount of a
pharmaceutical composition as aforementioned. It will be
appreciated that numerous of the above conditions also affect
non-human mammals, and treatment of such conditions is also within
the practice of the invention.
[0116] In a further preferred example of the invention, there is
provided a method for treating a non-human mammal to enhance the
growth and performance thereof, comprising administering an
effective amount of a pharmaceutical composition as aforementioned.
Enhancement of growth and performance includes, for example,
increased feed efficiency, improved milk yield or fertility, and
increased leanness.
[0117] A highly preferred example of the invention provides a
method wherein by secretion of growth hormone, gastrin, or glucagon
can be increased on a sustained basis in a mammal, including a
human, in need thereof, comprising administering a dose of a
pharmaceutical composition as aforementioned. According to this
example of the invention, physiologically adverse consequences of
artificial fluctuations in the circulating (or locally needed)
concentrations of these hormones can be avoided.
[0118] Although the pharmaceutical compositions and methods of the
invention are described primarily in terms of use with humans, and
non-human mammals, the skilled practitioner will immediately
appreciate that the invention, in many of its aspects, may be
usefully practiced with respect to birds, such as chickens and
turkeys, and also fishes.
Definitions
[0119] In connection with the practice of the invention, the
following definitions will generally apply.
[0120] The term "treating", as used herein, refers to reversing,
alleviating, inhibiting the progress of, or preventing the disorder
or condition to which such term applies, or one or more symptoms of
such disorder or condition. The term "treatment", as used herein,
refers to the act of treating, as "treating" is defined immediately
above.
[0121] The term "alkyl", as used herein, unless otherwise
indicated, includes saturated monovalent hydrocarbon radicals
having straight, branched or cyclic moieties or combinations
thereof. Similarly, the terms "alkenyl" and "alknyl" define
hydrocarbon radicals having straight, branched or cyclic moities
wherein at least one double bond, or at least one triple bond,
respectively, is present. Such definitions also apply when the
alkyl, alkenyl or alkynyl group is present within another group,
such as alkoxy or alkylamine.
[0122] The term "alkoxy", as used herein, includes O-alkyl groups
wherein "alkyl" is as defined above.
[0123] The term "halo", as used herein, unless otherwise indicated,
includes fluoro, chloro, bromo or iodo.
[0124] An "aryl" group as used herein, unless otherwise indicated,
includes an organic radical derived from a monocyclic or bicylic
(C.sub.6-C.sub.10) aromatic hydrocarbon compound by removal of a
hydrogen radical from a ring carbon of the aryl compound. An aryl
group is optionally substituted by one or more substituents
wherein, unless otherwise indicated, selection of each optional
substituent is independent of selection of any other optional
substituents, and perferably the number of optional substituents is
between 0 and 3, more preferably between 0 and 2. It will be
appreciated that the preferred number of substituents is determined
in part by facility of synthesis. Representative aryl groups are
phenyl and naphthyl.
[0125] A "heteroaryl" group as used herein, unless otherwise
indicated, includes an organic radical derived from a monocyclic or
bicyclic (C.sub.1-C.sub.9) aromatic heterocyclic compound by
removal of a hydrogen radical from a ring atom of the heteroaryl
compound, said ring atom being uncharged in said compound. A
heteroaryl group is optionally substituted by one or more
substituents wherein, unless otherwise indicated, selection of each
optional substituent is independent of selection of any other
optional substituents, and perferably the number of optional
substituents is between 0 and 3, more preferably between 0 and 2.
It will be appreciated that the preferred number of substituents is
determined in part by facility of synthesis. Representative
heteroaryl groups include furyl-, thienyl-, thiazolyl- pyrazolyl-,
isothiazolyl-, oxazolyl-, isoxazolyl-, pyrrolyl-, triazolyl-,
tetrazolyl-, imidazolyl-, 1,3,5-oxadiazolyl-, 1,2,4-oxadiazolyl-,
1,2,3-oxadiazolyl-, 1,3,5-thiadiazolyl-, 1,2,3-thiadiazolyl-,
1,2,4-thiadiazolyl-, pyridyl-, pyrimidyl-, pyrazinyl-,
pyridazinyl-, 1,2,4-triazinyl-1,2,3-triazinyl-, 1,3,5-triazinyl-,
pyrazolo[3,4-b]pyridinyl-, cinnolinyl-, pteridinyl-, purinyl-,
6,7-dihydro-5H-[1]pyrindinyl-, benzo[b]thiophenyl-,
5,6,7,8-tetrahydro-quinolin-3-yl-, benzoxazolyl-, benzothiazolyl-,
benzisothiazolyl-, benzisoxazolyl-, benzimidazolyl-,
thianaphthenyl-, isothianaphthenyl-, benzofuranyl-,
isobenzofuranyl-, isoindolyl-, indolyl-, indolizinyl-, indazolyl-,
isoquinolyl-, quinolyl-, phthalazinyl-, quinoxalinyl-,
quinazolinyl-, benzoxazinyl-; and the like.
[0126] A "cycloalkyl" group as used herein, unless otherwise
indicated, includes an organic radical derived from a monocyclic
(C.sub.3-C.sub.10)cycloalkyl compound, by removal of a hydrogen
radical from a ring carbon of the cycloalkyl compound. A cycloalkyl
group is optionally substituted by one or more substituents
wherein, unless otherwise indicated, selection of each optional
substituent is independent of selection of any other optional
substituents, and perferably the number of optional substituents is
between 0 and 3, more preferably between 0 and 2. It will be
appreciated that the preferred number of substituents is determined
in part by facility of synthesis. Representative cycloalkyl groups
include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl,
cycloheptyl, cyclopropenyl, cyclobutenyl, cyclopentenyl,
cyclohexenyl, cycloheptenyl, 1,3-cyclobutadienyl,
1,3-cyclopentadienyl, 1,3-cyclohexadienyl, 1,4-cyclohexadienyl,
1,3-cycloheptadienyl, 1,4-cycloheptadienyl,
1,3,5-cycloheptatrienyl, bicyclo[3.2.1]octane, bicyclo [2.2.1]
heptane, and the norborn-2-ene unsaturated form thereof. Thus, the
term cycloalkyl also includes cycloalkenyl groups having one or two
double bonds.
[0127] A "heterocycloalkyl" group as used herein, unless otherwise
indicated, includes an organic radical derived from a monocyclic
(C.sub.3-C.sub.10)heterocycloalkyl compound by removal of a
hydrogen radical from a ring atom of the heterocycloalkyl compound.
A heterocycloalkyl group is optionally substituted by one or more
substituents wherein, unless otherwise indicated, selection of each
optional substituent is independent of selection of any other
optional substituents, and perferably the number of optional
substituents is between 0 and 3, more preferably between 0 and 2.
It will be appreciated that the preferred number of substituents is
determined in part by facility of synthesis. Representative
heterocycloalkyl groups include pyrrolidinyl-, tetrahydrofuranyl-,
dihydrofuranyl-, tetrahydropyranyl-, pyranyl-, thiopyranyl-,
aziridinyl-, oxiranyl-, methylenedioxyl-, chromenyl-,
isoxazolidinyl-, 1,3-oxazolidin-3-yl-, isothiazolidinyl-,
1,3-thiazolidin-3-yl, 1,2-pyrazolidin-2-yl, 1,3-pyrazolidin-1-yl,
piperidinyl-, thiomorpholinyl-, 1,2-tetrahydrothiazin-2-yl,
1,3-tetrahydrothiazin-3-yl, tetrahydrothiadiazinyl-, morpholinyl-,
1,2-tetrahydrodiazin-2-yl, 1,3-tetrahydrodiazin-1-yl,
tetrahydroazepinyl, piperazinyl-, and chromanyl-.
[0128] In connection with the terms "aryl" group, "heteroaryl"
group, "cycloalkyl" group and "heterocycloalkyl" group, as herein
defined, the term "optionally substituted" means that one or more
chemically and pharmaceutically acceptable functional groups may be
bonded thereto. Such a group contributes properties useful to
production, storage, or use of the inventive compounds as
pharmaceuticals, or at least does not substantially negate their
pharmacological activity. Such suitable substituents may be
determined by those skilled in the art. Illustrative examples of
suitable substituents include, but are not limited to, hydroxy,
halo, amino, trifluoromethyl, carboxy, (C.sub.1-C.sub.6)alkoxy-,
(C.sub.1-C.sub.6)acyloxy-, (C.sub.1-C.sub.6)alkylamino-,
((C.sub.1-C.sub.6)alkyl).sub.2amino-, (C.sub.1-C.sub.6)acylamino-,
cyano, nitro, (C.sub.1-C.sub.6)alkyl-, (C.sub.2-C.sub.6)alkenyl-,
(C.sub.2-C.sub.6)alkynyl-, (C.sub.1-C.sub.6)acylamino-,
cyano(C.sub.1-C.sub.6)alkyl-,
trifluoromethyl(C.sub.1-C.sub.6)alkyl-,
nitro(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C- .sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acylamino-,
amino(C.sub.1-C.sub.6)acyl-,
amino(C.sub.1-C.sub.6)acyl(C.sub.1-C.sub.6)a- lkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)acyl-,
(C.sub.3-C.sub.10)cycloalkyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.2-C.sub.6)alkoxy(- C.sub.1-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-,
(C.sub.1-C.sub.6)alkyl(difluoromethylene)-,
(C.sub.1-C.sub.3)alkyl(difluoromethylene)(C.sub.1-C.sub.3)alkyl-,
(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)acyl-,
(C.sub.1-C.sub.6)alkylamin- o(C.sub.1-C.sub.6)acyl-,
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub- .6)acyl-,
(C.sub.6-C.sub.10)aryl-, (C.sub.1-C.sub.9)heteroaryl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroary- l(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl-,
(C.sub.6-C.sub.10)aryl(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkyl-
(C.sub.3-C.sub.10)cycloalkyl-,
(C.sub.3-C.sub.6)cycloalkyl(C.sub.1-C.sub.- 6)alkyl-,
(C.sub.3-C.sub.10)heterocycloalkyl-, (C.sub.3-C.sub.10)heterocyc-
loalkyl(C.sub.1-C.sub.6)alkyl-, hydroxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acyloxy(C.sub.2-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkoxy(- C.sub.2-C.sub.6)alkyl-,
piperazinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)acylamino(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)aryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.9)heteroaryl(C.sub.1-C.sub.6)alkoxy(C.sub.1-C.sub.6)alkyl--
, (C.sub.1-C.sub.6)alkylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylthio(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfinyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylsulfonyl(C.sub.1-C.sub.6)alkyl-,
(C.sub.6-C.sub.10)arylsulfonyl(C.sub.1-C.sub.6)alkyl-,
amino(C.sub.1-C.sub.6)alkyl-,
(C.sub.1-C.sub.6)alkylamino(C.sub.1-C.sub.6- )alkyl-, and
((C.sub.1-C.sub.6)alkyl).sub.2amino(C.sub.1-C.sub.6)alkyl.
[0129] Further aspects of the invention are described in accord
with the Detailed Description of the invention which follows
directly.
DETAILED DESCRIPTION OF THE INVENTION
[0130] According to the practice of the present invention, the
secretion of growth hormone (GH) from cells (such as those of the
anterior pituitary) is facilitated by inhibiting the
somatostatin-induced (and G-protein coupled) mechanisms that
otherwise naturally act to oppose said secretion. Without being
limited as to theory, these somatostatin-induced mechanisms act to
oppose both calcium ion and cyclic AMP-mediated signals that
otherwise enhance fusion with the cell membrane of cytoplasmic
granule structures that contain growth hormone, and thus the
subsequent release (secretion) of GH.
[0131] The present invention provides an effective approach to the
treatment of frailty in older persons, which may be caused, in
whole or part, by insufficient levels of growth hormone (GH), or
impairment of any of several downstream physiological effects
normally associated with growth hormone secretion.
[0132] It is generally recognized that GH is important to the
maintenance of connective and muscle tissue in adults, and may
help, to some extent, to increase muscle mass. Thus growth hormone
may be used to assist elderly patients even when growth hormone
levels per se are not the cause of, for example, weakness, or
attrition of muscle and connective tissues.
[0133] The practice of the invention benefits other patients, such
as children, when it can be demonstrated that secretion of GH is
inadequate, but is subject to enhancement. Deficiency in GH
secretion, or resultant GH activity, may arise in several ways. For
example, the gene sequence that encodes GH may be expressed in the
nucleus at subnormal levels, processing of resultant RNA transcript
or nascent polypeptide may be defective, or fusion of cytoplasmic
GH storage granules with the cell membrane (with resultant release
of GH) may be defective. Additionally, the patient may possess an
allele of the GH gene that encodesa mutant protein having less
biological activity. Alternatively, there may be an underlying
deficiency of GHRH, or a defect in the GHRH receptor, or defects in
the GHRP receptor or deficiency of its endogenous ligand, or in
respective signaling mechanisms. Additionally, there may be an
excess of somatostatin. In all such cases, the resultant
physiological deficiency can be treated by administration of the
pharmaceutical compounds of the invention.
[0134] In a further aspect of the invention, the performance and
growth rate of non-human mammals, such as livestock, is enhanced by
appropriate administration of the compounds disclosed herein.
Additionally, companion animals, and particularly older companion
animals also benefit upon administration of the present
compounds.
[0135] Under appropriate circumstances, somatostatin antagonists
may also exhibit the properties of agonists, and are thus
recognized as useful therapeutics in the treatment of diabetes, for
example, see H. Gronbaeck et al., Prog. Basic Clin Pharmacol.
(Basel), 10, pp. 103-128, 1996. Somatostatin agonists are also
recognized (see WO 98/44922) as useful therapeutics in the
treatment of, for example, diabetic retinopathy, acromegaly,
rheumatoid arthritis, neuropathic and visceral pain, irritable
bowel syndrome, Crohn's disease, and are useful to inhibit cell
proliferation associated with cancer, and to prevent restenosis
following angioplasty.
[0136] Additionally, sst2 ligands can evidence affinity for other G
protein-coupled receptors including the melanocortin receptor, the
MCH receptor, and MCR4. It is also expected that sst2 ligands will
evidence affinity for the MCH receptor SLC1 (somatostatin-like
receptor 1) since it is more than 50% homologous to sst2.
Accordingly, the compounds of the present invention are also useful
in the treatment of medical conditions mediated through these
receptors including, for example, treatment or prevention of
obesity, diabetes mellitus, erectile dysfunction and female sexual
dysfunction. Additionally, the compounds of the present invention
are useful to modulate appetite and metabolic rate. In particular,
the compounds of the present invention are useful to stimulate the
appetite of mammals for the treatment of diseases/disorders
associated with inappropriate food intake and weight loss, and for
example, to enhance growth and survivability of neonates in
livestock.
[0137] Although the compounds of the present invention act to
indirectly facilitate release of mature growth hormone from the
cytoplasmic storage granules of cells, additional therapeutic
substances are known that can directly enhance such secretion, and
further, can indirectly enhance production of growth hormone by via
enhanced expression of GH-encoding DNA in the cell nucleus. In this
regard, both growth hormone releasing peptide (GHRP) and growth
hormone releasing hormone (also known as growth hormone releasing
factor, GHRH/GRF) which act to release GH from cytoplasmic storage
granules have been mentioned. Since the release of GH from such
granules has been implicated as a signal triggering production of
additional GH protein in the cells, it is expected that GH levels
may be properly maintained in patients using a "push-pull"
approach.
[0138] Accordingly, a further preferred example of the invention
provides for the co-administration of the somatostatin-antagonist
compounds of the present invention and GHRP or GHRH, or other
substances of like effects. Medical treatment with GHRP (or GHRH)
alone is described in the following representative publications: M.
Thorner et al., Journal Of Clinical Endocrinology And Metabolism,
81(3), pp. 1189-1196, 1996; S. G. Celia et al., Peptides, 16(1),
pp. 81-86, 1995; M. A. Bach et al., Journal Of The American
Geriatrics Society, 44(9), S10, 1996; and J. A. Aloi et al.,
Journal Of Clinical Endocrinology And Metabolism, 79(4), pp.
943-949, 1994.
[0139] Further, since growth hormone is very labile, and its
half-life in the body is very short, it is difficult to provide a
safe dosing program for direct administration of growth hormone
itself, which avoids wide swings in circulating levels of the
hormone. Current sustained release technologies for direct
administration of growth hormone can be improved upon. In this
regard, the practice of the present invention is particularly
valuable to the clinician, since by only indirectly raising GH
levels, the hormone's release profile remains, at least in part,
under the control of the body's own regulatory feedback systems,
and fluctuations in the levels of circulating GH are damped over
time. Additionally, the compounds of the present invention may
themselves be administered by sustained release mechanisms. It is
also recognized that patients sometimes inadvertently skip doses,
and various technologies exist to provide continuous dosing via the
digestive tract including, for example, osmotic systems. In this
regard, the pharmaceutical compositions of the invention are
preferably administered according to the technology disclosed in
U.S. Pat. No. 4,612,008.
[0140] In the preferred practice of the invention, compounds show
selectivity for the sst2 receptor compared with other receptor
subtypes, for example sst1, sst3, sst4 and sst5. This selectivity
minimizes the chance that other molecular biological or biochemical
pathways will be adversely affected while growth hormone secretion
is being upregulated. Most preferably, the affinity of a compound
for the sst2 type receptor should be at least about 10 times
greater than for receptors of the other sst-subtypes.
[0141] It should be noted that the compounds of the invention may
work by more than one mechanism, including those unrelated to
interaction at an sst-type receptor, and the utility of the present
compounds in the practice of the invention, including for use in
treating other disease states not particularly mentioned herein, is
not limited by any particular theory as described herein or by
those theories that is generally recognized by those skilled in the
art.
[0142] Additionally, the compounds of the present invention may
interact beneficially with sst-type receptors other than sst2, and
may provide therapeutic benefits by acting as somatostatin
agonists, rather than antagonists, at sst2 or other sst-type
receptors.
[0143] As aforementioned the compounds of this invention include
all conformational isomers (e., cis and trans isomers, whether or
not involving double bonds), tautomers, and all optical isomers of
compounds of the formula I (eq., enantiomers and diastereomers), as
well as racemic, diastereomeric and other mixtures of all such
isomers.
[0144] With respect to the design of the compounds of the
invention, particular features involving conformational and optical
isomerism are of note.
[0145] In connection with the above general formula, particular
structural features are of note. In formula 1.
[0146] Additionally, where substitution by one or more halo groups
is permitted above, a preferred example is by one or two halo
atoms. Preferably the halo atom(s) is selected from chlorine and
fluorine. Generally speaking, trifluoromethyl is the preferred
species of trifluoro(C.sub.1-C.sub.6)alkyl group. Where
substitution by one or more trifluoromethyl groups is permitted, it
is preferred that only a single trifluoromethyl group be
incorporated.
[0147] Additionally, many of the groups of the present compounds
may be optionally substituted. As aforementioned, such substituents
contribute properties useful to production, storage, or use of the
inventive compounds as pharmaceuticals, or at least does not
substantially negate their pharmacological activity. It will be
appreciated that selection of optional substituents is further
guided by principles recognized in the art, and/or is capable of
validation through the use of the assays described in the present
specification.
Preferred Structural Features
[0148] Group Z may exist in the form of various optical isomers. In
the below structures (i) to (iv), optically active centers are
indicated. 9
[0149] According to the practice of the invention it is preferred,
but not required, that the L-configuration be present at the carbon
atom in the carboline provided by the alpha-carbon of the component
tryptophan, and similarly that the L-configuration be present at
the carbon atom of 1,2,3,4-tetrahydroisoquinoline that is provided
by the alpha-carbon of component, phenylalanine, both as indicated
below. 10
[0150] With respect to the remaining optically active centers, each
resultant optically active species is an example of the present
invention.
[0151] Referring to structures (i) and (ii), J may be S, O, --NH--,
or NCH.sub.3. Such structures Z may be prepared by the
Pictet-Spengler reaction from amino acids that are commercially
available, or are readily prepared.
[0152] According to a still further embodiment of the invention,
group Z may be more broadly defined (group Z') than as otherwise
presented herein. In a group Z', the exemplified
(C.sub.1-C.sub.9)heteroaryl group of structures Z(i) or Z(ii), that
is, indole, benzofuran, or benzothiophene is subject to replacement
by any other (C.sub.1-C.sub.9)heteroaryl group within the
definition thereof as aforementioned, which group may be also be
optionally substituted. Similarly for group Z', the exemplified
(C.sub.6-C.sub.10) aryl group (phenyl) in structures Z(iii) and
Z(iv) may be replaced with naphthyl, which may also be optionally
substituted.
[0153] When group "W" of formula (I) is option (a), it is preferred
that this group thereof have a stereospecificity at the indicated
position (which corresponds to the .alpha.-carbon of an amino
acids), such that L-amino acids, or other structures having the
same absolute stereospecificity, are defined. 11
[0154] In preferred examples, the W group defines an L-lysine group
or a (C.sub.1-C.sub.8)alkyl ester thereof. In further preferred
examples, the W group is L-diaminopimelic acid, L-canavanine,
L-ornithine, L-2,4-diaminobutyric acid, L-5-hydroxylysine,
L-epsilon-N-methyllysine, or (C.sub.1-C.sub.8)alkyl esters thereof.
In further preferred examples, the W group is an L-arginine group
or a (C.sub.1-C.sub.8)alkyl ester thereof.
[0155] In an additional embodiment of the invention, group W
according to formula (a) is replaced by L-histidine,
L-3-methylhistidine, or an (C.sub.1-C.sub.8)alkyl ester thereof,
even though such structures are not derivable from the depicted
formula.
[0156] Again with respect to group W option (a), in a preferred
example, R.sup.3 is selected from option (ii) or option (iii)
thereof 12
[0157] and each of R.sup.6, R.sup.7' and R.sup.7" if present, is
(C.sub.1-C.sub.6) alkyl for example, methyl, ethyl, and
t-butyl.
[0158] When the "Z" component is a .beta.-carbolene (for example a
"bridged tryptophan"), such as options (i) and (ii), the "W"
component is preferably selected from L-lysine or a
(C.sub.1-C.sub.8)alkyl ester thereof, L-diaminopimelic acid,
L-canavanine, L-ornithine, L-2,4-diaminobutyric acid,
L-5-hydroxylysine, L-epsilon-N-methyllysine, or
(C.sub.1-C.sub.8)alkyl esters of any thereof.
[0159] Similarly, when the "Z" component is a
tetrahydroisoquinoline (for example, a "bridged phenylalanine"),
such as options (iii) and (iv), the "W" component is preferably
selected from L-Arginine, or a (C.sub.1-C.sub.8)alkyl ester
thereof. For the purposes of the invention, tetrahydroisoquinolines
can be formed using, for example, 2-fluorophenylalaninyl-,
3-fluorophenylalaninyl-, 4-fluorophenylalaninyl- or
diphenylalaninyl-.
[0160] Alternatively, group W can represent option (b) moiety,
wherein structures such as the following are preferred (refer to
Schemes 5A, 5B) 13
[0161] Additionally, many of the groups of the present compounds
may be "optionally substituted", as previously defined. As
aforementioned, such substituents contribute properties useful to
production, storage, or use of the inventive compounds as
pharmaceuticals, or at least does not substantially negate their
pharmacological activity. It will be appreciated that selection of
optional substituents is further guided by principles recognized in
the art, and/or is capable of validation through the use of the
assays described in the present specification.
Pharmaceutical Formulations
[0162] The compounds of the present invention that are basic in
nature are capable of forming a wide variety of different salts
with various inorganic and organic acids. Although such salts must
be pharmaceutically acceptable for administration to animals, it is
often desirable in practice to initially isolate the compound of
the present invention from the reaction mixture as a
pharmaceutically unacceptable salt and then simply convert the
latter back to the free base compound by treatment with an alkaline
reagent and subsequently convert the latter free base to a
pharmaceutically acceptable acid addition salt. The acid addition
salts of the base compounds of this invention are readily prepared,
for example, by treating the base compound with a substantially
equivalent amount of the chosen mineral or organic acid in an
aqueous solvent medium or in a suitable organic solvent, such as
methanol or ethanol. Upon careful evaporation of the solvent, the
desired solid salt is readily obtained. The desired acid salt can
also be precipitated from a solution of the free base in an organic
solvent by adding to the solution an appropriate mineralor organic
acid.
[0163] Those compounds of the present invention that are acidic in
nature, are capable of forming base salts with various
pharmacologically acceptable cations. Examples of such salts
include the alkali metal or alkaline-earth metal salts and
particularly, the sodium and potassium salts. These salts are all
prepared by conventional techniques. The chemical bases which are
used as reagents to prepare the pharmaceutically acceptable base
salts of this invention are those which form non-toxic base salts
with the acidic compounds of the present invention. Such non-toxic
base salts include those derived from such pharmacologically
acceptable cations as sodium, potassium calcium and magnesium, etc.
These salts can easily be prepared by treating the corresponding
acidic compounds with an aqueous solution containing the desired
pharmacologically acceptable cations, and then evaporating the
resulting solution to dryness, preferably under reduced pressure.
Alternatively, they may also be prepared by mixing lower alkanolic
solutions of the acidic compounds and the desired alkali metal
alkoxide together, and then evaporating the resulting solution to
dryness in the same manner as before. In either case,
stoichiometric quantities of reagents are preferably employed in
order to ensure completeness of reaction and maximum yields of the
desired final product.
[0164] In a preferred example of the invention, the compounds of
the present invention may be formulated with additional
pharmaceutically active substances that directly or indirectly (1)
facilitate production and storage in cells of additional growth
hormone, or precursor polypeptides thereof, or (2) facilitate
release of GH. Such additional substances include growth hormone
releasing peptide (GHRP), growth hormone releasing hormone (GHRH),
pituitary adenylate cyclase activating polypeptide (PACAP),
dopaminergic agonists (e.g. bromocriptine), beta-adrenergic
agonists (e.g. isoproterenol) and alpha 1-adrenergic agonists (e.g.
methoxamine). For background information see E. O Soyoola et al.,
Proceedings of the Society for Experimental Biology & Medicine,
207(1), pp. 26-33, 1994; V. Locatelli et al., Pediatric Research,
36(2), pp. 169-74, 1994; and B. Velkeniers et al., Journal of
Endocrinology, 143(1), pp. 1-11, 1994.
[0165] Equivalently, the additional pharmaceutically active
substances may be provided as a separate formulation which is
co-administered, or administered at some other timepoint(s) in the
course of treatment.
[0166] This invention also encompasses pharmaceutical compositions
containing prodrugs of compounds of the formula 1. This invention
also encompasses methods of treating or preventing disorders that
can be treated or prevented by decreasing the levels of
somatostatin comprising administering prodrugs of compounds of the
formula 1. Compounds of formula I having free amino, amido, hydroxy
or carboxylic groups can be converted into prodrugs. Prodrugs
include compounds wherein an amino acid residue, or a polypeptide
chain of two or more (e.g., two, three or four) amino acid residues
which are covalently joined through peptide bonds to free amino,
hydroxy or carboxylic acid groups of compounds of formula 1. The
amino acid residues include the 20 naturally occurring amino acids
commonly designated by three letter symbols and also include,
4-hydroxyproline, hydroxylysine, demosine, isodemosine,
3-methylhistidine, norvalin, beta-alanine, gamma-aminobutyric acid,
citrulline, homocysteine, homoserine, ornithine and methionine
sulfone. Prodrugs also include compounds wherein carbonates,
carbamates, amides and alkyl esters which are covalently bonded to
the above substituents of formula I through the carbonyl carbon
prodrug sidechain.
[0167] One of ordinary skill in the art will also appreciate that
when using the compounds of the invention in the treatment of a
specific disease, that the compounds of the invention may be
combined with various existing therapeutic agents used for that
disease, or for other metabolically related or unrelated disease
states that may occur simultaneously. As aforementioned, the
additional pharmaceutically active substances may be provided as a
separate formulation which is co-administered, or administered at
some other timepoint(s) in the course of treatment.
[0168] The compounds of the invention can also be used in
combination with existing therapeutic agents such as the
above-mentioned growth hormone secretagogues for the treatment of
growth hormone deficiency.
[0169] For the treatment of growth hormone deficiency, the
compounds of the invention may be combined with agents such as
recombinant growth hormone which is marketed by Genentech and
licensees (Neutropin, Genotropin and Protropin), Bio-Technology
General and licensees (Zomacton, Growject, Elvetium and SciTropin),
Novo Nordisk (Norditropin), LG Chem (Eutropin), Ares Serono (Saizen
and Serostim), Eli Lilly Co (Humatrope), Monsanto (Posilac brand of
bovine growth hormone) and Alpharma (Reporcin brand of swine growth
hormone).
[0170] The compounds of the invention can also be used in
combination with existing therapeutic agents such as Geref
(sermorelin, GHRH) from Serono Laboratories Inc.
[0171] The compounds of the invention can also be used in
combination with existing therapeutic agents such as anabolic
steroids, e.g. androisoxazol androstanolone (DHT,
dihydrotestosterone, Stanolone, Anabolex, Andractrim), bolandiol,
bolasterone, bolazin, boldenone (Equipoise), calusterone, clostebol
(chlortestosterone, Steranabol, Alfa Trofodermin, Dermanabol,
Trofodermin, Trofoseptine), danazol (Cyclomen, Danocrine),
dehydrochlormethyltestosterone (turinabol, Oral-turinabol),
drostanolone (dromostanolone, Drolban, Masterid, Masteril,
Masteron, Metormon, Premastril), estradiol, ethylestrenol,
fluoxymesterone (Halotestin, Ora-Testryl, Android-F), formebolone,
furazabol (Miotolon), mestanolone, mesterolone (Proviron,
Pluriviron), methandienone (methandrostenolone, Metaboline),
methandriol, methenolone (Primobolan), methyltestosterone
(Methandren, Premarin with methyltestosterone, Android, Oreton,
Testred, Methyltestosterone tabs, Geri-Bons, Geri-tabs, Dermonal),
mibolerone (Cheque), nandrolone (Deca-Durabolin, Durabolin,
Nandrabolin, Anabolin, Androlone, Hybolin, Nandrobolic),
norclostebol, norethandrolone (Nilevar), oxabolone, oxandrolone
(Anavar), oxymesterone (Oranabol), oxymetholone (Anapolon 50,
Androyd, Anadrol, Anasteron, Dynasten, Oxitosona, Plenastril,
Synasteron, Zenalosyn), penmesterol, prasterone, quinbolone,
stanozolol (Winstrol, Winstrol-V, Stromba, Strombaject),
stenbolone, testosterone (Malogen, Delatestryl, Malogen, Neo-pause,
PMS-testosterone Enanthate, Andriol, Duogex, Neo-Pause,
Climacteron, Orchisterone-P, Oreton, Anadiol, Anatest, Testos-100,
Heifer-aid, Synovex-H), tibolone, trenbolone (Parabolan, Finaject)
or zeranol.
[0172] The compounds of the invention can also be used in
combination with existing therapeutic agents such as Somazon
(mecasermin, recombinant insulin-like growth factor 1) from
Fujisawa.
[0173] For the treatment of older patients with osteoporosis,
suitable agents to be used in combination with the compounds of the
invention include standard non-steroidal anti-inflammatory agents
(hereinafter NSAID's) such as piroxicam, diclofenac, propionic
acids such as naproxen, flubiprofen, fenoprofen, ketoprofen and
ibuprofen, feramates such as mefenamic acid, indomethacin,
sulindac, apazone, pyrazolones such as phenylbutazone, salicylates
such as aspirin, COX-2 inhibitors such as celecoxib and rofecoxib,
analgesics and intraarticular therapies such as corticosteroids and
hyaluronic acids such as hyalgan and synvisc.
[0174] The compounds of the present invention may also be used in
combination with osteoporosis agents such as lasofoxifene,
raloxifene, droloxifene or fosomax and immunosuppressant agents
such as FK-506 and rapamycin.
[0175] The compounds of the present invention may also be used in
combination with immunostimulant agents for the treatment of
reduced immune function.
[0176] The compounds of the present invention may also be used in
combination with fertility agents such as human menopausal
gonadotropin, chorionic gonadotropin, follicle stimulating hormone,
nafarelin, triptorelin, cetrorelix, and ganirelix for the treatment
of infertility.
[0177] The compounds of the present invention may also be used in
combination with AIDS therapies for the treatment of AIDS-related
complex.
[0178] The compounds of the present invention may also be used in
combination with anti-tumor necrosis factor agents such as
infliximab (TNF monoclonal antibody) or etanercept (soluble TNF
receptor) for the treatment of cachexia.
[0179] The compounds of the present invention may also be used in
combination with potassium channel blockers, beta-blockers,
anticoagulants or vasodilators for the treatment of heart
disease.
[0180] The compounds of the present invention may also be used in
combination with angiotensin II (ATII) antagonists or
erythropoietin for the treatment of renal failure.
[0181] For administration to livestock, the compounds of the
invention may also be used in combination with feed additives such
as antibiotics (e.g. monensin, lasalocid, salinomycin,
semduramicin, narasin, maduramicin, virginiamycin, polymixin,
efrotomycin, avoparcin, lincomycin, bacitracin, bambermycins,
novobiocin, erythromycin, oleandomycin, streptomycin, tylosin,
penicillin, tetracycline, oxytetracycline, chlortetracycline,
carbadox, olaquindox, neomycin, moenomycin, avilamycin, and
flavophospholipol), repartitioning agents, beta-agonists ( e.g.
Paylean, ractopamine, from Elanco), and also amiterol, bambuterol,
bitolterol, broxaterol, buphenine, carbuterol, cimaterol,
clenbuterol, clorprenaline, colterol, denopamine, dioxethedrine,
dioxifedrine, dobutamine, dopexamine, doxaminol, etanterol,
fenoterol, flerobuterol, formoterol, hexoprenaline, ibuterol,
imoxiterol, isoetarine, isoxsuprine, levisoprenaline, mabuterol,
mesuprine, metaterol, methoxyphenamine, nardeterol, orciprenaline,
picumeterol, pirbuterol, prenalterol, procaterol, protokylol,
quinprenaline, rimiterol, ritodrine, salbutamol, salmeterol,
terbutaline, tretoquinol, tulobuterol, xamoterol and
zilpaterol.
[0182] The compositions of the present invention may be formulated
in a conventional manner using one or more pharmaceutically
acceptable carriers. Thus, the active compounds of the invention
may be formulated for oral, buccal, intranasal, parenteral (e.g.,
intravenous, intramuscular or subcutaneous) or rectal
administration or in a form suitable for administration by
inhalation or insufflation. The active compounds of the invention
may also be formulated for sustained delivery.
[0183] For oral administration, the pharmaceutical compositions may
take the form of, for example, tablets, chewable. tablets, or
capsules prepared by conventional means with pharmaceutically
acceptable excipients such as binding agents (e.g., pregelatinized
maize starch, polyvinylpyrrolidone or hydroxypropyl
methylcellulose); fillers (e.g., lactose, microcrystalline
cellulose or calcium phosphate); lubricants (e.g., magnesium
stearate, talc or silica); disintegrants (e.g., potato starch or
sodium starch glycolate); or wetting agents (e.g., sodium lauryl
sulphate). The tablets may be coated by methods well known in the
art. Liquid preparations for oral administration may take the form
of, for example, solutions, syrups or suspensions, or they may be
presented as a dry product for constitution with water or other
suitable vehicle before use. Such liquid preparations may be
prepared by conventional means with pharmaceutically acceptable
additives such as suspending agents (e.g., sorbitol syrup, methyl
cellulose or hydrogenated edible fats); emulsifying agents (e.g.,
lecithin or acacia); non-aqueous vehicles (eq, almond oil, oily
esters or ethyl alcohol); and preservatives (e.g., methyl or propyl
p-hydroxybenzoates or sorbic acid).
[0184] For buccal administration, the composition may take the form
of tablets or lozenges formulated in conventional manner, or
blended with petfood or animal feed, or as a pre-mix for blending
with animal feed.
[0185] The active compounds of the invention may be formulated for
parenteral administration by injection, including using
conventional catheterization techniques or infusion. Formulations
for injection may be presented in unit dosage form, e.g., in
ampules or in multi-dose containers, with an added preservative.
The compositions may take such forms as suspensions, solutions or
emulsions in oily or aqueous vehicles, and may contain formulating
agents such as suspending, stabilizing and/or dispersing agents.
Alternatively, the active ingredient may be in powder form for
reconstitution with a suitable vehicle, e.g., sterile pyrogen-free
water, before use.
[0186] The active compounds of the invention may also be formulated
in rectal compositions such as suppositories or retention enemas,
e.g., containing conventional suppository bases such as cocoa
butter or other glycerides.
[0187] For intranasal administration or administration by
inhalation, the active compounds of the invention are conveniently
delivered in the form of a solution or suspension from a pump spray
container that is squeezed or pumped by the patient or as an
aerosol spray presentation from a pressurized container or a
nebulizer, with the use of a suitable propellant, e.g.,
dichlorodifluoromethane, trichlorofluoromethane,
dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In
the case of a pressurized aerosol, the dosage unit may be
determined by providing a valve to deliver a metered amount. The
pressurized container or nebulizer may contain a solution or
suspension of the active compound. Capsules and cartridges (made,
for example, from gelatin) for use in an inhaler or insufflatdr may
be formulated containing a powder mix of a compound of the
invention and a suitable powder base such as lactose or starch.
[0188] A proposed dose of the active compounds of the invention for
oral, parenteral or buccal administration to the average adult
human is 0.1 to 100 mg of the active ingredient per unit dose which
could be administered, for example, 1 to 4 times per day.
[0189] Aerosol formulations for treatment of the conditions
referred to above in the average adult human are preferably
arranged so that each metered dose or "puff" of aerosol contains 20
.mu.g to 1000 .mu.g of the compound of the invention. The overall
daily dose with an aerosol will be within the range 0.1 mg to 100
mg. Administration may be several times daily, for example 2, 3, 4
or 8 times, giving for example, 1, 2 or 3 doses each time.
[0190] Injected doses are preferably administered from about once a
month, up to about 1 to 4 times per day, at an individual dosing of
0.01-1 mg/kg (of active ingredient) and may be intramuscular,
intravenous, or subcutaneous, for example.
[0191] As is well recognized, the precise dose, and method and
timing of administration thereof, are capable of determination by
those skilled in the art, and depend upon numerous factors
including the activity of the therapeutic compound, the properties
of the formulation thereof, the nature and location of the target
tissue, and the particulars of the disease state as it exists in a
particular patient. Additionally, when the compounds of the present
invention are administered to a patient with additional
pharmaceutically active substances, one or more pharmaceutical
compositions may be used to deliver all of the active agents, which
may be administered together, or at different times, as determined
by those skilled in the pharmaceutical or medical arts.
[0192] The following reaction schemes illustrate preparation of
compounds of the present invention. It will be appreciated that
certain groups represented by letters (R.sub.2, for example) in the
Schemes do not always correspond with similarly defined component
groups of the formula (I) compounds themselves, since certain
functionalities of the reactants are modified, by definition, when
the products are formed R.sup.1, R.sub.2 and R.sub.3 typically
represent (C.sub.1-C.sub.6),alkyl groups, whether primary,
secondary, or tertiary, but can also be other groups such as
(C.sub.6-C.sub.10)aryl or benzyl, for example. 14 15 16 1718 19
20
[0193] General Reaction Conditions
[0194] Generally speaking, the compounds of the present invention
are made by a series of condensation reactions in which certain
reactive groups are appropriately protected, and the sequence of
condensation is controlled. Typically, alternative pathways exist
to the same products, as the reactants may be coupled in more than
one sequence. The starting materials herein are commercially
available or are readily prepared. Compounds such as BOC
derivatives or CBZ derivatives are also readily prepared.
[0195] Referring to Scheme 1A, the compounds of formula 1 which
include an L-arginine moiety, may be prepared from the compounds of
formula 2 by removal of the guanidine-protecting nitro group via a
reduction reaction using formic acid as reducing agent in the
presence of palladium on carbon. In a typical procedure, the
reaction mixture is stirred overnight under nitrogen, filtered, and
the solvent then removed under reduced pressure. Recovered material
may then be triturated with diethylether, and dried overnight under
high vacuum to yield the final product. Although nitro is the
preferred protecting group, Boc may also be used, in which case
suitable reaction conditions for deprotection are stirring with
trifluoracetic acid or hydrochloric acid.
[0196] Again referring to Scheme IA, the compounds of formula 2 may
be prepared by reaction of compounds 3 with a compound that
provides group A, that is, A'-(CH.sub.2).sub.n--,
A'-(CH.sub.2).sub.nSO.sub.2--, and A'-(CH.sub.2).sub.nCO--, where n
is 0 to 4; and wherein A' is selected from (C.sub.6-C.sub.10)aryl-,
or (C.sub.1-C.sub.9)heteroaryl-. Suitable forms of group A include
acid chlorides and sulfonyl chlorides which are reacted with
compounds 3 under well known conditions. In the case where A is
A'-(CH.sub.2).sub.n--, A may first be provided as an
A'-(CH.sub.2).sub.n-1CHO group, which is subject to reductive
amination on compound 3 using sodium cyanoborohydride.
[0197] .beta.-carbolene compounds of formula 3 are prepared from
compounds of formula 4 using the appropriate aldehyde by
application of Pictet-Spengler reaction. As will be discussed
below, the Pictet-Spengler cyclization can be performed at
different points depending in the overall scheme. Generally this
reaction is conducted under strongly acidic conditions.
[0198] Compounds of formula 4 are prepared from compounds of
formula 5 by hydrolysis (deprotection) of the BOC group at the
.alpha.-amino group of the amino acid. Compounds of formula 5 are
prepared by condensation of of the compounds of formula 6 and 7,
for example in the presence of
1,3-dimethylaminopropyl-3-ethylcarbodiimidehydrochloride (EDC),
hydroxybenzotriazole, and dimethylaminopyridine. The reaction
mixture may then be washed successfully with portions of of 10%
aqueous hydrochloric acid solution, followed by washes with 50%
saturated sodium bicarbonate solution, and saturated brine. The
resulting product 5 may then be dried over anhydrous magnesium
sulfate, filtered, and the solvent removed under reduced
pressure.
[0199] In the preferred practice of the invention, compounds of
formulas 6 and 7 include amino acid moieties which confer
peptide-like structure on the final product compounds, consistent
with their activity as somatostatin analogs. Compound 7 may
represent any one of several suitably protected amino acids wherein
the the carboxyl groups thereof is protected by a suitable alkyl
group (R.sub.2). The stereospecificity atithis subregion of the
product compound, defined as "W" herein, is determined by the
stereospecificity of the participating amino acid. In the practice
of the invention, stereospecificity corresponding to an L-amino
acid is preferred. Compound 6 typically represents a BOC-protected
reactant having an indole, benzofuran, or benzothiophene moiety,
with a preferred example defining tryptophan or a derivative
thereof.
[0200] Scheme 1B defines a similar reaction pathway except that
reactant 13 represents a BOC-protected phenylalanine, or derivative
thereof, and the Pictet-Spengler reaction thus produces a
tetrahydroisoquinoline group.
[0201] As aforementioned, when the "Z" component is a
tetrahydroisoquinoline (a "bridged phenylalanine"), such as
Z-options (iii) and (iv), the "W" component is preferably selected
from L-Arginine, or a (C.sub.1-C.sub.8)alkyl ester thereof. For the
purposes of the invention, tetrahydroisoquinolines can be formed
using, for example, 2-fluorophenylalaninyl-,
3-fluorophenylalaninyl-, 4-fluorophenylalaninyl- or
diphenylalaninyl-.
[0202] Note that L-lysine is preferably selected to provide the "W"
component, when Trp derivatives (whether L or R) as a carbolene are
used to provide the "Z" component.
[0203] Additionally, when the "Z" component is a .beta.-carbolene
(for example, a "bridged tryptophan"), such as Z-options (i) and
(ii), the "W" component is preferably selected from L-lysine or a
(C.sub.1-C.sub.8)alkyl ester thereof, L-diaminopimelic acid,
L-canavanine, L-ornithine, L-2,4-diaminobutyric acid,
L-5-hydroxylysine, L-epsilon-N-methyllysine, or
(C.sub.1-C.sub.8)alkyl esters of any thereof. Under such
circumstances, the tryptophan derivative may be L or R, defined at
the .alpha.-carbon thereof. When the "W" component is so selected,
synthesis via a variant of Scheme 1A is preferred. For reaction
with compound 6, compound 7 is replaced by a CBZ derivative, in
which the alpha carboxy group is also protected. 21
[0204] The synthesis is then continued, as outlined in Scheme 1A,
except that the final reduction (reaction 2.fwdarw.1) is
accomplished using palladium/carbon with H.sub.2 to remove CBZ.
[0205] Product compounds are also preferably made according to
Scheme 3 (product 23), wherein condensation of a CBZ-protected
lysine (28) and a BOC-protected heteroaryl amino acid (29) using
EDC is depicted. Scheme 3 is also useful where reactant 29
contributes a phenylalanine moiety, with the Pictet-Spengler
reaction being similarly conducted as reaction 26.fwdarw.25.
[0206] As aforementioned, and indepedent of the general formulas
otherwise provided, the W component of the product compound may
also define a histidine residue, preferably L-histidine, which may
be optionally substituted, preferably by a methyl group, so that W
is 2-methylhistidine, for example. Additionally, and making
reference to group R.sup.10, which can modify the W group as
otherwise defined herein, the alpha carboxy group of the optionally
substituted histidine residue can comprise a (C.sub.1-C.sub.8)alkyl
ester or benzyl ester.
[0207] In a variation on the above procedures, the Pictet-Spengler
reaction can become the first step in the synthetic pathway. In
Scheme 2, compounds 21 are first produced from compounds 22 using
this reaction. As before, the reaction is preferably conducted
under strongly acidic conditions. The pathway is equally applicable
when compound 22 instead represents phenylalanine or a derivative
thereof. Product compounds 15 result, and the chemistry described
above for attachment of the A group (here 17.fwdarw.16) also
applies. Many simple carbolines and tetrahydroisoquinoline
caroboxylic acids are commercially available, or are readily
prepared.
[0208] Referring again to Scheme 2, where Z is defined by option
(ii), and R.sup.17 is, for example, hydrogen, 22
[0209] it is apparent that compound 17 may also represent
essentially the final product of the process. In this case (Scheme
2), the aldehyde R.sup.14CHO is selected to not only close the ring
in the Pictet-Spengler reaction, but also to provide the A group.
If the .alpha.-amino group of the .beta.-carbolene is present as
free NH, the protecting BOC group is then removed from compound 17
under acidic conditions. In the case where the .alpha.-amino group
of the .beta.-carbolene is alkyated in the final product, the alkyl
group is preferably added to compound 17 by reductive deamination
of an appropriate aldehyde, followed by reduction with sodium
cyanoborohydride. The BOC group is removed under acidic conditions.
This approach also works where Z is defined by option (iv).
[0210] Scheme 4 is also efficiently used both in cases where group
A is to be attached at the nitrogen atom of group Z that is
contributed by the amino acid .alpha.-amino group, and where
attachment of group A is to a ring carbon of Z that is proximal to
said nitrogen. In Scheme 4, group R.sub.1 of compound 33 represents
attachment of the compound to oxime resin (see Example 2).
Localizing compounds on the resin facilitates purification
procedures and also limits unwanted side reactions. Compounds 32
are produced from compounds 31 by application of the
Pictet-Spengler reaction using appropriate aldehyde, R.sup.14CHO.
The overall pathway is similar to that outlined in Scheme 2, except
that localization on the oxime resin permits the use of fewer
blocking groups on the reactants.
[0211] As mentioned similarly for Scheme 2, incorporation of group
A into the design of R.sup.14CHO permits simultaneous placement of
group A, and also ring closure according to the Pictet-Spengler
reaction. In such cases, Z groups according to option (ii) thereof
are formed, and R.sup.14 groups A'-(CH.sub.2).sub.n-- where n is 0
to 4; and A' is selected from (C.sub.6-C.sub.10)aryl-, and
(C.sub.1-C.sub.9)heteroaryl- are incorporated. It should be noted
however that Scheme 4 is substantially less efficient where group Z
is patterned on a phenylalanine moiety as conditions of stronger
acidity would typically be needed, releasing reactants from the
oxime resin. In such cases, resort to Scheme 2 (using a
phenylalanine-containing moiety in the reaction 22.fwdarw.21, under
strong acid conditions) is preferred. BOC is removed from compounds
30 under acidic conditions which permit retention of the ester
group at R.sub.3, if this is desired.
[0212] Should the product compound instead include a group A
attached at the .alpha.-amino group of Z, such may be introduced
into compound 30 using a sulfonyl chloride, an acid chloride, or by
reductive amination of an appropriate aldehyde, as
aforementioned.
[0213] Schemes 5A and 5B provide approaches to group "W", in the
general structure of formula (I), where W is alternative (b).
23
[0214] and in particular, outline representative syntheses of
component W wherein Q is, for example, either cyclohexane or
pyridine. Thus, Schemes 5A and 5B permit synthesis of compounds
similar to compounds of Schemes 1-4, but wherein the representative
arginine or lysine moiety thereof is replaced by a moiety that
includes a cyclohexane or pyridine group, for example.
[0215] Referring to Scheme 5A, compounds of formula 35 may be
prepared from compounds of 36 by reduction with hydrogen under
appropriate conditions. Compounds of formula 36 may be prepared
from compounds of formula 37 via reaction using NaN.sub.3 to
displace the mesylate ester of compounds 37. Compounds 37 may be
prepared from compounds 38 with mesyl (methanesulfonyl) chloride
under basic conditions, for example, in
triethylamine/dichloromethane at 0.degree. C., in good yield.
Compounds 38 may be prepared from compounds 39 by reduction at the
carboxyl group thereof using BH.sub.3. Compounds 39, having the
stereospecificity indicated in Scheme 5A, are prepared from racemic
compounds 41 by chiral resolution with stereospecific
.alpha.-methylbenzylamine 40, followed by selective purification,
such as by crystallization. Compounds 41 may be prepared from the
corresponding aromatic compounds 42 by reduction with hydrogen, for
example, under appropriate conditions. Compounds 42 in turn are
prepared from the corresponding (unprotected) compounds 43 by
reaction with BOC anhydride under standard conditions. Finally,
compounds 44 may be prepared from available starting materials 44,
by reduction of the cyano group with hydrogen over a Raney nickel
preparation. In Scheme III(b), advantage is taken of available
starting materials to generate compounds of the formula 34 in 2
steps, first from compounds of formula 45 using BOC anhydride.
Compounds 45 are generated from compounds of formula 46 by
reduction of both cyano groups, again with hydrogen and Raney
nickel as catalyst.
Additional Embodiments of the Invention
[0216] In additional embodiments of the invention, group A is
replaced by one of the following structures: 24
[0217] wherein A" is selected from the groups defined for A' above;
25
[0218] wherein R.sup.2 is
[0219] (a) selected from H, (C.sub.1-C.sub.8)alkyl-, and
phenyl(CH.sub.2)--, wherein said alkyl and phenyl groups are each
optionally substituted by one or more halo groups, or
[0220] (b) is selected from groups defined for A' above; and
[0221] A" is selected from the groups defined for A' above; and
26
[0222] wherein each occurrence of R.sup.1 is independently selected
from hydroxy, halo, (C.sub.1-C.sub.8)alkyl- optionally substituted
by one or more halo, and (C.sub.1-C.sub.8)alkoxy- optionally
substituted by one or more halo;
[0223] In the practice of the invention, the above compounds were
less effective than some of the aforementioned compounds under the
particular assay conditions chosen.
The Following are Representative Compounds of the Invention
EXAMPLE 1
5-Guanidino-2-{[2-(toluene-4-sulfonyl)-1,2,3,4-tetrahydro-isoquinoline-3-c-
arbonyl]-amino}-pentanoic acid methyl ester
[0224] Preparation of Tos-L-Tic
[0225] 993 mg (24.8 mmole) sodium hydroxide was dissolved in 50 mL
of water, after which 2.00 gm (11.29 mmol) of
S-1,2,3,4-tetrahydro-3-isoquin- oline carboxylic acid (Tic) was
added with stirring until dissolved. A solution of 2.152 gm (11.29
mmole) tosyl chloride in 50 mL of diethyl ether was then added, and
the 2 phase reaction stirred vigorously overnight. The aqueous
layer was separated out and acidified to pH 2.0 with 10% aqueous
hydrochloric acid. The product was then extracted with three 100 mL
portions of diethyl ether, and the combined organic layers were
washed with saturated sodium chloride, dried over magnesium
sulfate, and then filtered. The solvent was removed under reduced
pressure to yield 3.367 gm (90%) of product.
[0226] Step 2
[0227] To a solution of 100 mg of Tos-L-Tic (0.30 mmol), 122 mg of
Arg(NO.sub.2)--OMe HCl (0.45 mmol), 61 mg of hydroxybenzotriazole
(0.39 mmol), and 184 mg of 4-dimethylaminopyridine (1.51 mmol) in
50 mL of methylene chloride was added 174 mg (0.78 mmol) of
1,3-dimethylaminopropyl-3-ethylcarbodiimide hydrochloride. After
stirring for 15 hours, 100 mL of additional methylene chloride was
added to the reaction, and it was washed three times with 30 mL
portions of 10% aqueous hydrochloric acid solution, then twice with
20 mL of 50% saturated sodium bicarbonate solution, and finally
once with 20 mL of saturated brine. The product was then dried over
anhydrous magnesium sulfate, filtered, and the solvent removed
under reduced pressure. The product was next dissolved in 20 mL of
methanol, and 230 mg of 10% palladium on carbon was added under
nitrogen, followed by addition of 1 mL of formic acid, after which
the reaction stirred for 15 hours. The catalyst was filtered off,
and the solvent was then removed rapidly under reduced pressure to
yield 22 mg of product. This material can also be synthesized by
coupling a suitably protected arginine fragment with a suitably
protected Tic fragment, deprotecting the Tic amino group,
condensing this material with p-toluenesulfonyl chloride, and
finally, deprotecting the arginine sidechain.
EXAMPLE 2
General Procedure for Synthesis of .beta.-Carbolene Analogs
[0228] 27
[0229] Oxime resin (Novabiochem, 0.6 mmol, g; 150 mg. 0.09 mmol)
was placed into a 10 mL Bio-Rad (Richmond, Calif.) poly-prep column
and washed sequentially with CH.sub.2Cl.sub.2 (3 times.times.5 mL),
dimethylformamide (2 times.times.5 mL), and CH.sub.2Cl.sub.2 (3
times.times.5 mL), respectively. Then, a solution consisting of
N-Boc-L-Trp (109 mg, 0.36 mmol), DIC (diisopropylcarbodiimide, 0.36
mmol, 69 mg), and 10% DMAP (dimethylaminopyridine, 5 mg, 0.036
mmol) in 3 ml of CH.sub.2Cl.sub.2. was added, and the mixture was
agitated by rotation at room temperature for 16 hours. The mixture
was then washed sequentially with CH.sub.2Cl.sub.2 (3 times.times.5
mL), DMF (2 times.times.5 mL), ethanol (2 times.times.5 mL), and
finally CH.sub.2Cl.sub.2 (3 times.times.5 mL). 3 mL of 25% TFA in
CH.sub.2Cl.sub.2 was then added, and the mixture rotated for 30
minutes, followed by washing with CH.sub.2Cl.sub.2 (3 times.times.5
mL), DMF (2 times.times.5 mL), 10% DIEA in CH.sub.2Cl.sub.2
(diisopropylethylamine, 2 times.times.5 mL), ethanol (2
times.times.5 mL), and finally CH.sub.2Cl.sub.2 (3 times.times.5
mL).
[0230] At this point, the synthesis continues with step
33.fwdarw.32 of Scheme 4. A solution of aldehyde R.sup.14CHO (0.9
mmol, 10 equiv to reactant 33) in 25% TFA in CH.sub.2Cl.sub.2 (3
mL) was added, and rotated for 6 h. The mixture was then washed
sequentially with CH.sub.2Cl.sub.2 (3 times.times.5 mL), DMF (2
times.times.5 mL), 10% DIEA in CH.sub.2Cl.sub.2 (2 times.times.5
mL), ethanol (2 times.times.5 mL), and CH.sub.2Cl.sub.2 (3
times.times.5 mL), respectively. A solution of H-Lys(Boc)OMe.HCl
(94 mg, 0.36 mmol) and triethylamine (50 uL, 0.36 mmol) in 3 ml
CH.sub.2Cl.sub.2 was then added, followed by rotation at room
temperature for 48 hours. The contents of the tube were then
filtered and concentrated. The crude product material was further
purified on a Biotage Quad 3 system (1:3 hexane:ethyl acetate) to
isolate 10-40% product. Resulting products were stirred in 4 M HCl
in dioxane for 20 minutes, concentrated, and tritrated with
isopropylether to deliver the HCl salts.
EXAMPLE 3
6-Amino-2-{[1-(3-benzyloxy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl]-amino}-hexanoic acid methyl ester
[0231] Following generally the procedure of Example 2, the compound
was prepared from 3-benzyloxybenzaldehyde to give 24 mg of a 1.9:1
mixture of cis:trans adducts. MS (APCl) M+541.6.
[0232] A sample of cis and trans isomers was separated by HPLC to
give 8 mg of the cis isomer,
6-Amino-2-{[1-(S)-(3-benzyloxy-phenyl)-2,3,4,9-tetr-
ahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexanoic acid methyl
ester, and 3.5 mg of the corresponding trans adduct
6-Amino-2-{[1-(R)-(3-benzylo-
xy-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl]-amino}-hexan-
oic acid methyl ester.
EXAMPLE 4
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl]-amino}-hexanoic acid methyl ester
[0233] Step 1--Initial Preparation of
6-tert-Butoxycarbonylamino-2-(S)-[(2-
,3,4,9-tetrahydro-1H-.beta.-carboline-3-(S)-carbonyl)-amino]-hexanoic
acid methyl ester.
[0234] L-norharmane (.beta.-carbolene, 1.08 g, 5 mmol) was added to
a solution of 1 N NaOH (20 mL) and saturated NaHCO.sub.3 (20 mL),
followed by sonication until completely dissolved. Benzyl
chloroformate (1 mL, 7 mmol) was added and the resulting solution
was stirred overnight. The aqueous phase was washed with ether (2
times.times.25 mL), acidified to pH 1, and extracted with
CH.sub.2Cl.sub.2 (3 times.times.50 mL). The combined extracts were
dried (MgSO.sub.4) and evaporated to give 1.56 g
1,3,4,9-tetrahydro-.beta.-carboline-2,3-dicarboxylic acid 2-benzyl
ester. The acid (1.4 g, 4 mmol) was dissolved into CH.sub.2Cl.sub.2
(30 mL) and TEA (1 mL, 8 mmol), after which EDC
(1,3-dimethylaminopropyl-3-ethylcarbo- diimide hydrochloride, 802
mg, 4.2 mmol) and H-L-Lys(Boc)-OMe (1.24 g, 4.2 mmol) were added.
After stirring overnight, the mixture was diluted with water (50
mL) and extracted with CH.sub.2Cl.sub.2 (3 times.times.50 mL). The
combined extracts were washed sequentially with citric acid,
saturated NaHCO.sub.3 and brine solutions, dried over (MgSO.sub.4),
and evaporated to give 1.82 g of dipeptide. A sample (1.2 g, 2
mmol) of the CBZ-protected dipeptide was combined with Pd/C (25 mg,
0.2 mmol) in ethanol (20 mL) and agitated under H.sub.2 (40 psi)
overnight. The resulting mixture was filtered through celite and
evaporated to deliver 0.961 g product,
6-tert-Butoxycarbonylamino-2-(S)-[(2,3,4,9-tetrahydro-1H-
-.beta.-carboline-3-(S)-carbonyl)-amino]-hexanoic acid methyl
ester, MS (APCl) M+459.6
[0235] Step 2
[0236] A solution of
6-tert-Butoxycarbonylamino-2-(S)-[(2,3,4,9-tetrahydro-
-1H-.beta.-carboline-3-(S)-carbonyl)-amino]-hexanoic acid methyl
ester (37 mg, 0.08 mmol), TEA (0.21 mL, 0.15 mmol) and
toluene-4-sulfonyl chloride (19 mg, 0.1 mmol) in CH.sub.2Cl.sub.2
(2 mL) wa stirred at room temperature overnight. The mixture was
diluted with water (10 mL), extracted with CH.sub.2Cl.sub.2, washed
with brine, dried (over MgSO.sub.4) and evaporated to give 39 mg
crude product. Purification by flash chromatography (SiO2 gel; 2:1
EtOAc/hexanes) delivered 24 mg pure Boc-protectected product. A
sample (15 mg) of this material was stirred in a 50% solution of
TFA in CH.sub.2Cl.sub.2 for 30 minutes and evaporated to afford 12
mg of the TFA salt. MS (APCl) M+513.5.
EXAMPLE 5
6-Amino-2-{[2-(biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl]-amino}-hexanoic acid methyl ester
[0237]
6-Amino-2-{[2-(biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-ca-
rboline-3-carbonyl]-amino}-hexanoic acid methyl ester was prepared
according to the above procedure from
6-tert-Butoxycarbonylamino-2-(S)-[(-
2,3,4,9-tetrahydro-1H-.beta.-carboline-3-(S)-carbonyl)-amino]-hexanoic
acid methyl ester (37 mg, 0.08 mmol) and biphenyl-4-sulphonyl
chloride (24 mg, 0.1 mmol) to deliver 6 mg of the TFA salt. MS
(APCl) M+575.5
EXAMPLE 6
6-Amino-2-{[2-(biphenyl-4-carbonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-
-3-carbonyl]-amino}-hexanoic acid methyl ester
[0238] A solution of
6-tert-butoxycarbonylamino-2-(S)-[(2,3,4,9-tetrahydro-
-1H-.beta.-carboline-3-(S)-carbonyl)-amino]-hexanoic acid methyl
ester (37 mg, 0.08 mmol), TEA (0.1 mL, 0.12 mmol), PPAA (n-propane
phosphonic acid cyclic anhydride, 64 uL, 50% solution in EtOAc, 0.1
mmol) and 4-phenyl benzoic acid (20 mg, 0.1 mmol) in
CH.sub.2Cl.sub.2 (2 mL) was stirred overnight at room temperature.
The mixture was diluted with water (10 mL), extracted with
CH.sub.2Cl.sub.2, washed with brine, dried (MgSO.sub.4) and then
evaporated to give 39 mg crude product. Purification by flash
chromatography (SiO2 gel; 2:1 EtOAc/hexanes) delivered 22 mg pure
Boc-protectected product. A sample (14 mg) of this material was
stirred in a 50% solution of TFA in CH.sub.2Cl.sub.2 for 30 minutes
and evaporated to afford 12 mg of the TFA salt. MS (APCl)
M+539.5.
EXAMPLE 7
6-Amino-2-[(2-biphenyl-4-yl-methyl-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl)-amino]-hexanoic acid methyl ester
[0239]
6-tert-butoxycarbonylamino-2-(S)-[(2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-(S)-carbonyl)-amino]-hexanoic acid methyl ester (23 mg,
0.05 mmol), 4-phenyl benzaldehde (14 mg, 0.075 mmol) and
NaCNBH.sub.3 (7 mg, 0.1 mmol) were stirred in methanol (5 mL)
overnight. The mixture was filtered through celite and evaporated.
The resulting residue was taken up in CH.sub.2Cl.sub.2, washed with
water and then brine, dried (MgSO.sub.4), and evaporated to afford
33 mg crude amine. Purification by flash chromatography (SiO2 gel;
1:1 ETOAc/hexanes) delivered 18 mg Boc-protected adduct. A sample
(15 mg) of this material was stirred in 50% TFA in CH.sub.2Cl.sub.2
for 30 minutes and evaporated to give 15 mg of the TFA salt. MS
(APCl) M+525.4.
EXAMPLE 8
6-Amino-2-({1-[4-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1H-
-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl ester
[0240] Following generally the procedure of Example 2, the compound
was prepared from 4-(4-trifluoromethyl-phenoxy)-benzaldehyde to
give 21 mg of a 1.7:1 mixture of cis:trans adducts MS M+595.6.
EXAMPLE 9
6-Amino-2-{[1-(4-butyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-ca-
rbonyl]-amino}-hexanoic acid methyl ester
[0241] Following generally the procedure of Example 2, the compound
was prepared from 4-n-butylbenzaldehyde to give 6 mg of product. MS
M+491.6.
EXAMPLE 10
6-Amino-2-{[1-(4-pyrrolidin-1-yl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carb-
oline-3-carbonyl]-amino}-hexanoic acid methyl ester
[0242] Following generally the procedure of Example 2, the compound
was prepared from 4-pyrrolidin-1-yl-benzaldehyde to give 10 mg of a
1.8:1 mixture of cis:trans adducts. MS M+504.2.
EXAMPLE 11
6-Amino-2-({1-[2-(4-isopropyl-phenyl)-1-methyl-ethyl]-2,3,4,9-tetrahydro-1-
H-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl
ester
[0243] Following generally the procedure of Example 2, the compound
was prepared from 3-(4-Isopropyl-phenyl)-2-methyl-propionaldehyde
to give 3.5 mg of a mixture of cis:trans adducts. MS M+519.5.
EXAMPLE 12
6-Amino-2-({1-[3-(4-trifluoromethyl-phenoxy)-phenyl]-2,3,4,9-tetrahydro-1H-
-.beta.-carboline-3-carbonyl}-amino)-hexanoic acid methyl ester
[0244] Following generally the procedure of Example 2, the compound
was prepared from 3-(4-trifluoromethyl-phenoxy)-benzaldehyde to
give 4 mg of a 1.6:1 mixture of cis:trans isomers. MS M+595.6.
EXAMPLE 13
6-Amino-2-{[1-(1-methyl-3-phenyl-butyl)-2,3,4,9-tetrahydro-1H-.beta.-carbo-
line-3-carbonyl]-amino}-hexanoic acid methyl ester
[0245] Following generally the procedure of Example 2, the compound
was prepared from 1-methyl-3-phenyl-butanal to give 5 mg of product
as a mixture of isomers. MS M+505.1.
EXAMPLE 14
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl]-amino}-hexanoic acid tert-butyl ester
[0246]
6-Amino-2-{[2-(toluene-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-car-
boline-3-carbonyl]-amino}-hexanoic acid tert-butyl ester was
prepared from
6-Amino-2-{[2-(biphenyl-4-sulfonyl)-2,3,4,9-tetrahydro-1H-.beta.-carbolin-
e-3-carbonyl]-amino}-hexanoic acid tert-butyl ester (25 mg) and
toluene-4-sulfonyl according to the above procedures to deliver 15
mg of the TFA salt. MS (APCl) M+555.5.
EXAMPLE 15
5-Guanidino-2-{[2-(toluene-4-sulfonyl)-1,2,3,4-tetrahydro-isoquinoline-3-c-
arbonyl]-amino}-pentanoic acid tert-butyl ester
EXAMPLE 16
6-Amino-2-({2-[4-(2-oxo-2,3-dihydro-benzoimidazol-1-yl)-piperidine-1-carbo-
nyl]-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carbonyl}-amino)-hexanoic
acid tert-butyl ester.
EXAMPLE 17
6-Amino-2-({2-[(1H-indol-3-yl)-acetyl]-2,3,4,9-tetrahydro-1H-.beta.-carbol-
ine-3-carbonyl}-amino)-hexanoic acid methyl ester
[0247] A solution of
6-tert-butoxycarbonylamino-2-(S)-[(2,3,4,9-tetrahydro-
-1H-.quadrature.-carboline-3-(S)-carbonyl)-amino]-hexanoic acid
methyl ester (23 mg, 0.05 mmol), TEA (20 uL, 0.2 mmol), PPAA
(propanephosphonic acid cyclic anhydride, 35 uL, 50% solution in
EtOAc, 0.1 mmol) and (1H-Indol-3-yl)-acetic acid (20 mg, 0.07 mmol)
in CH.sub.2Cl.sub.2 (2 mL) was stirred overnight at room
temperature. The mixture was diluted with water (10 mL), extracted
with CH.sub.2Cl.sub.2, washed with brine, dried (MgSO.sub.4) and
then evaporated to give 31 mg crude product. Purification by flash
chromatography (SiO2 gel; 2:1 EtOAc/hexanes) delivered 18 mg pure
Boc-protectected product. A sample (5 mg) of this material was
stirred in a 50% solution of HCl in EtOH for 30 minutes and
evaporated to afford 5 mg of the HCl salt. MS (APCl) M+516.4.
EXAMPLE 18
6-Amino-2-{[1-(4-isopropyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carboline--
3-carbonyl]-amino}-hexanoic acid methyl ester
[0248] Following generally the procedure of Example 2, the compound
was prepared from 3-I-propyl-benzaldehyde to give 3 mg of a 1:1
mixture of cis:trans adducts. MS M+476.6.
EXAMPLE 19
6-Amino-2-{[1-(3-trifluoromethyl-phenyl)-2,3,4,9-tetrahydro-1H-.beta.-carb-
oline-3-carbonyl]-amino}-hexanoic acid methyl ester
[0249] Following generally the procedure of Example 2, the compound
was prepared from 4-(3-trifluoromethyl-phenoxy)-benzaldehyde to
give 4 mg of a 1.6:1 mixture of cis:trans adducts MS M+595.6.
EXAMPLE 20
6-Amino-2-[(1-anthracen-9-yl-2,3,4,9-tetrahydro-1H-.beta.-carboline-3-carb-
onyl)-amino]-hexanoic acid methyl ester
[0250] Following generally the procedure of Example 2, the compound
was prepared from anthracene-9-carbaldehyde. 8 mg of a 2.7:1
mixture of cis:trans adducts was recovered. MS (APCl) M+535.6.
Biological Assays
[0251] Various types of somatostain agonists are well known in the
art, and the capacity of a compound of the present invention to act
as an agonist, an antagonist, or as either, depending on
physiological circumstances, can be predicted from the assays which
are known in the art and/or described below. For example,
measurement of cyclic-AMP, growth hormone release, microphysiometry
responses, cell proliferation or protein kinase activity can be
measured in cultured pituitary cells, cell lines or other cells
such as neuroblastoma cells that express somatostatin receptors,
and cells transfected with recombinant somatostatin receptors
including transfected yeast cells. (Y. C. Patel et al., Biochemical
& Biophysical Research Communications, 198(2), pp. 605-612,
1994; M. G. Cattaneo et al., FEBS Letters, 397(2-3), pp. 164-168,
1996; J. A. Koenig et al., British Journal of Pharmacology, 120(1),
pp. 45-51, 1997; D. Djordjijevic et al., Endocrinology, 139(5), pp.
2272-2277, 1998; W. R. Baumbach et al., Molecular Pharmacology,
54(5), pp. 864-73, 1998).
[0252] Generally, somatostatin or agonists thereof demonstrate
inhibitory activity, hence a stimulus is first applied (e.g.
forskolin for cyclic-AMP) and the inhibitory effect of somatostatin
observed. Antagonists reverse the inhibitory effects of
somatostatin.
[0253] The ability of compounds of formula (I), and the
pharmaceutically acceptable salt, solvates or hydrate thereof
(hereinafter referred to as the compounds of the present invention)
to act as somatostatin antagonists, or agonists, and consequently
to demonstrate their effectiveness in the treatment of disease
states, is shown by the following assays.
EXAMPLE 21
Bovine ("b")sst2 Binding Assay
[0254] The present example describes an assay for binding of
pharmaceutically useful somatostatin agonists and antagonists at
the bovine sst2 receptor.
[0255] The methods for culturing Neuro2A cells and measuring
competitive binding potency (IC.sub.50) were similar to those
described by J. A. Koenig et al., "Somatostatin receptors in
Neuro2A neuroblastoma cells: operational characteristics", British
J. Pharmacol., 120, 45-51, 1997, with the following
modifications.
[0256] Binding assays were conducted 72 hours after transiently
transfecting the Neuro2A cells with a plasmid (PCl-bsst2)
containing an insert coding for the bovine sst2 receptor, placed
downstream of the cytomegalovirus promoter. In the transfection
step, 6.5.times.10.sup.6 Neuro2A cells were added in 35 ml of media
to each tissue culture flask (162 cm.sup.2 surface area). The next
day, transfection was conducted using Fugene 6 (Boehringer
Mannheim, 1 814 443) according to the manufacturer's directions.
The Fugene 6 (30 .mu.l/flask) was equilibrated with 8 .mu.g of
PCl-bsst2 plasmid, and added to the Neuro2A cells in the absence of
fetal bovine serum. After 3 hours, fresh serum-containing media was
added. The assay buffer was modified to contain 50 mM HEPES, 5 mM
MgCl.sub.2, 1 mg/ml bovine serum albumin (BSA), 0.02 mg/ml
bacitracin, and 10 .mu.M each of aprotinin, leupeptin and AEBSF.
The transfected Neuro2A cells were dissociated in the absence of
trypsin/EDTA, in ice cold assay buffer (5.5 ml/flask), and cells
were homogenized in a 55 ml Wheaton Dounce homogenizer (15-20
strokes). Membrane preparations were stored in aliquots at
-70.degree. C. Competitive binding assays and separation of bound
from free radioactivity were conducted in polyethyleneimine-soaked
Millipore 96 Well GF/C Filterplates, (MAFC NOB10). An amount of
membrane was used that bound approximately 20% of
[.sup.125I]-somatostatin 14 tracer (Amersham, IM161), which was
added to all wells at 15,000 cpm/well (approximately 15 nCi/well).
Somatostatin was included in each experiment as positive control,
at 7 concentrations from 0.0042 to 1.667 nM, and test compounds
were included at 7 concentrations from 33 nM to 13.33 .mu.M. The
reaction volume was 300 .mu.l and the incubation was conducted for
1 hour at 37.degree. C. Non-specific binding was defined using 0.83
.mu.M somatostatin 14. The incubation was terminated by vacuum
filtration through the glass fiber plate bottom, followed with a
250 .mu.l wash with assay buffer minus BSA and protease inhibitors.
The plate bottom was then sealed, scintillation fluid was added
(Wallac Supermix, 250 .mu.l/well), and radioactivity was measured
in a 96 well microtiter liquid scintillation counter.
[0257] IC.sub.50 values are determined by polynomial regression and
analzyed using a MACRO program. An IC.sub.50 value of less than
about 5 .mu.M is preferred.
EXAMPLE 22
Rat Pituitary Assay for Somatostatin Receptor Antagonists
[0258] This assay is designed to quantitate the activity of
antagonists of somatostatin that interact directly at the
somatostatin receptor. The assay facilitates discovery of agents
which increase growth hormone secretion by modulating the
inhibitory effects of somatostatin. As aforementioned, somatostatin
(also abbreviated SRIF) inhibits GH secretion in the anterior
pituitary by binding to a high affinity membrane-bound (and
G-protein coupled) receptor which is coupled negatively to adenyl
cyclase, thereby reducing intracellular levels of cAMP that would
otherwise facilitate, for example, secretion/release of GH from
cytoplasmic granules. Vasoactive intestinal peptide (VIP) is one of
several endogenous peptides that stimulates GH secretion by binding
to a high affinity membrane-bound receptor coupled to a G
protein-dependent signal transduction pathway. VIP activates
adenylate cyclase and produces increased intracellular cAMP levels.
These peptides may be involved in the coordinate regulation of GH
secretion under physiologic conditions and be mediated through
cAMP. The cell line used in the screen is a clonal pituitary cell
that synthesizes and secretes GH in response to VIP and SRIF, and
many other regulatory hormones, as expected for normal pituitary
cells. The screen is designed to quantitate the ability of test
agents to reverse SRIF's inhibition of the elevated intracellular
cAMP levels produced by VIP.
[0259] In particular, cyclic AMP (cAMP) content of the pituitary
cell line GH.sub.4C.sub.1 was used to differentiate somatostatin
agonists from antagonists. The method was similar to that described
by L. J. Dorflinger et al. ("Somatostatin inhibits vasoactive
intestinal peptide-stimulated cyclic adenosine monophdsphate
accumulation in GH pituitary cells", Endocrinology, 113, pp.
1541-50, 1983 ) with the following modifications. Aliquots (50
.mu.l) of GH.sub.4C.sub.1 cell suspension at 1-2 million cells/ml
were added to 50 .mu.l of each solution of test compound in
Adenylyl Cyclase Activation FlashPlate.RTM. Assay plates from
NEN.TM. Life Science Products (catalog SMP004A). Putative
somatostatin agonists or antagonists were typically tested at
concentrations of 10, 1 and 0.1 .mu.M, in the presence of 100 nM
vasoactive intestinal peptide (VIP; Sigma V3628) and 10 nM
somatostatin 14.(cell culture tested, Sigma S1763). The
FlashPlates.RTM., which are coated with antibody against cAMP and
contain scintillant integral to the plastic, are supplied as part
of a kit with all necessary reagents to estimate cAMP content of
whole cell preparations, including Stimulation Buffer, Detection
Buffer, cAMP Standard, and [.sup.125I]-cAMP Tracer. This afforded a
convenient way to conduct a homogenous immunoradiometric assay of
cAMP content in cells lysed in situ, following incubation of the
cells with test compound. cAMP content in the GH.sub.4C.sub.1 cells
was determined according to the manufacturer's instructions, by
comparison with standards at concentrations from.10 to 1,000 nM
cAMP. In this assay, VIP increased cAMP content of the
GH.sub.4C.sub.1 cells, and somatostatin caused a partial
inhibition. Test compounds acting as somatostatin antagonists were
detected by their tendancy to increase cAMP content in comparison
to control wells containing VIP and somatostatin but no test
compound. Somatostatin agonists conversely decreased cAMP
content.
[0260] IC.sub.50 values are determined by polynomial regression and
analzyed using a MACRO program. An IC.sub.50 value of less than
about 5 .mu.M is preferred.
EXAMPLE 23
Effect of a Somatostatin Antagonist on GH Release in 12 kg Pigs
[0261] Studies indicate that concentrations of GH increase in small
pigs within 10 minutes of administration of somatostatin
antagonists, and then return to pre-treatment levels within 40
minutes post-administration.
[0262] The following protocol describes the effects of various
doses of a somatostatin antagonist on release of endogenous porcine
GH (or pST, porcine somatatrophin). Methods used to evaluate
effects of compounds on plasma GH concentrations in barrows
(castrated male pigs) were similar to those reported by M. J.
Estienne et al., "Methyl-D,L-aspartate-induced growth hormone
secretion in barrows: possible mechanisms of action", Journal of
Animal Science 74, pp. 597-602, 1996, with the following
modifications. Forty cross-bred barrows weighing approximately 12
kg were acclimatized for 2 days at 10 pigs per 36 sq. ft. pen, 4
pens per study, with feed (PS-9 swine starter diet) and water
provided ad libitum. To enhance uniformity, two pigs/pen were
eliminated based on being smallest or largest, or for health
reasons, bringing the group size to 8 pigs/treatment. An equal
number of pigs in each pen received 1 of 4 possible treatments at
random, i.e. one of 3 doses of test compound or diluent alone.
Compounds diluted in approximately 1 ml/pig sterile saline were
administered by intramuscular injection into the rear leg (ham),
about 1 minute after collection of the first blood sample into 7 ml
heparinized evacuated tubes via jugular venepuncture. Blood samples
were similarly collected at 10 minute intervals up to 40 minutes
after injection of test compound or diluent. Plasma was separated
by centrifugation and frozen at -20.degree. C.).
EXAMPLE 24
RIA Procedure for Determination of GH Levels in Plasma
[0263] The present assay is used to determine GH levels (for
example, porcine GH or canine GH) in plasma samples.
[0264] The double antibody radioimmunoassay (RIA) used to determine
porcine GH concentrations in plasma samples was similar to that
described by Y. N. Sinha et al., "Studies of GH secretion in mice
by a homologous radioimmunoassay for mouse GH", Endocrinology, 91,
pp.784-92, 1972, and that of F. Cocola et al., "A rapid
radioimmunoassay method of growth hormone in dog plasma",
Proceedings of the Society for Experimental Biology and Medicine,
151, pp. 140-14, 1976. Modifications were as follows. Native
porcine GH (pGH) for radioiodination as tracer, canine GH for use
as standard (cGH; AFP-1983B; the aminoacid sequence of canine and
porcine GH are the same), and primary antibody (monkey anti-cGH;
AFP-21452) were supplied by A. F. Parlow, Harbor UCLA Medical
Center. Recombinant porcine GH from Biogenesis was alternatively
used for radioiodination as tracer. Radioiodinations were conducted
by Biomedical Technologies Inc, Stoughton, Mass. Primary antibody
(1:50,000 or 1:100,000 final dilution), normal monkey serum (ICN
55988; 1:1,000 final dilution), and plasma sample or standard (0.08
to 2.5 ng cGH/tube) were mixed and incubated for 2 hours at ambient
temperature, then tracer (10,000 cpm/tube) was added and the
incubation continued for a further 20 hours at ambient temperature
in a total volume of 500 .mu.l. Secondary antibody (goat
anti-monkey IgG ICN 55418; final dilution 1:160) and
polyethyleneglycol 8,000 (final concentration 44 mg/ml) were added
and mixed in a final volume of 1.6 ml. Tubes were incubated at
4.degree. C. for 2 hours with shaking, then they were centrifuged,
supernates discarded, and the gamma-emission of the pellets
determined.
[0265] Plasma growth hormone concentrations, expressed as ng/ml,
were calculated from the standard line following log-logit
transformation.
* * * * *