U.S. patent application number 10/758524 was filed with the patent office on 2004-07-15 for fatty acid elongase 3-ketoacyl coa synthase polypeptides. This patent application is currently assigned to Miami University, an Ohio corporation. Invention is credited to Blacklock, Brenda J., Jaworski, Jan G..
Application Number | 20040139498 10/758524 |
Document ID | / |
Family ID | 22782454 |
Filed Date | 2004-07-15 |
United States Patent Application | 20040139498 |
Kind Code | A1 |
Jaworski, Jan G. ; et al. | July 15, 2004 |
Elongase KCS polypeptides with altered substrate specificity and/or catalytic activity are disclosed. Such elongase KCS polypeptides are effective for producing very long chain fatty acids (VLCFA) fatty acids. Also disclosed are nucleic acids encoding elongase KCS polypeptides, and yeast and plants expressing these polypeptides.
Inventors: | Jaworski, Jan G.; (Oxford, OH) ; Blacklock, Brenda J.; (Oxford, OH) |
Correspondence Address: |
Scott R. Pribnow Cargill, Incorporated 15407 McGinty Road West Wayzata MN 55391 US |
Assignee: | Miami University, an Ohio
corporation |
Family ID: | 22782454 |
Appl. No.: | 10/758524 |
Filed: | January 15, 2004 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
10758524 | Jan 15, 2004 | |||
09877476 | Jun 8, 2001 | |||
6713664 | ||||
60210326 | Jun 8, 2000 | |||
Current U.S. Class: | 800/281 ; 435/193; 435/419; 536/23.2 |
Current CPC Class: | C07K 2319/00 20130101; C12N 9/1029 20130101 |
Class at Publication: | 800/281 ; 435/193; 435/419; 536/023.2 |
International Class: | A01H 001/00; C12N 015/82; C07H 021/04; C12N 009/10; C12N 005/04 |
Sequence CWU 1
1
56 1 1709 DNA Arabidopsis thaliana CDS (1)...(1518) 1 atg acg tcc
gtt aac gtt aag ctc ctt tac cgt tac gtc tta acc aac 48 Met Thr Ser
Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt
ttc aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe
Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25
30 gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc
144 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu
35 40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act
gtt ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr
Val Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg
gtt tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro
Val Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt cca cca ccg cat
ctc aaa gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His
Leu Lys Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc tac caa
ata aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln
Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac gtg gca tgt gat
gat ccg tcc tcg ctc gat ttc ctg agg aag 384 Arg Asn Val Ala Cys Asp
Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 att caa gag cgt
tca ggt cta ggt gat gag acg tac agt cct gag gga 432 Ile Gln Glu Arg
Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140 ctc att
cac gta cca ccg cgg aag act ttt gca gcg tca cgt gaa gag 480 Leu Ile
His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155
160 aca gag aag gtt atc atc ggt gcg ctc gaa aat cta ttc gag aac acc
528 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Glu Asn Thr
165 170 175 aaa gtt aac cct aga gag att ggt ata ctt gtg gtg aac tca
agc atg 576 Lys Val Asn Pro Arg Glu Ile Gly Ile Leu Val Val Asn Ser
Ser Met 180 185 190 ttt aat cca act cct tcg cta tcc gct atg gtc gtt
aat act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val
Asn Thr Phe Lys 195 200 205 ctc cga agc aac atc aaa agc ttt aat cta
gga gga atg ggt tgt agt 672 Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu
Gly Gly Met Gly Cys Ser 210 215 220 gct ggt gtt att gcc att gat ttg
gct aaa gac ttg ttg cat gtt cat 720 Ala Gly Val Ile Ala Ile Asp Leu
Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aac act tat gct
ctt gtg gtg agc act gag aac atc aca caa ggc 768 Lys Asn Thr Tyr Ala
Leu Val Val Ser Thr Glu Asn Ile Thr Gln Gly 245 250 255 att tat gct
gga gaa aat aga tca atg atg gtt agc aat tgc ttg ttt 816 Ile Tyr Ala
Gly Glu Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt
gtt ggt ggg gcc gcg att ttg ctc tct aac aag tcg gga gac cgg 864 Arg
Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Ser Gly Asp Arg 275 280
285 aga cgg tcc aag tac aag cta gtt cac acg gtc cga acg cat act gga
912 Arg Arg Ser Lys Tyr Lys Leu Val His Thr Val Arg Thr His Thr Gly
290 295 300 gct gat gac aag tct ttt cga tgt gtg caa caa gaa gac gat
gag agc 960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp
Glu Ser 305 310 315 320 ggc aaa atc gga gtt tgt ctg tca aag gac ata
acc aat gtt gcg ggg 1008 Gly Lys Ile Gly Val Cys Leu Ser Lys Asp
Ile Thr Asn Val Ala Gly 325 330 335 aca aca ctt acg aaa aat ata gca
aca ttg ggt ccg ttg att ctt cct 1056 Thr Thr Leu Thr Lys Asn Ile
Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gaa aag ttt
ctt ttt ttc gct acc ttc gtc gcc aag aaa ctt 1104 Leu Ser Glu Lys
Phe Leu Phe Phe Ala Thr Phe Val Ala Lys Lys Leu 355 360 365 cta aag
gat aaa atc aag cat tac tat gtt ccg gat ttc aag ctt gct 1152 Leu
Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375
380 gtt gac cat ttc tgt att cat gcc gga ggc aga gcc gtg atc gat gag
1200 Val Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Glu 385 390 395 400 cta gag aag aac tta gga cta tcg ccg atc gat gtg
gag gca tct aga 1248 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt ggg aat act
tca tct agc tca att tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gaa tta gca tac ata gag
gca aag gga aga atg aag aaa ggg aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gct tgg cag
att gct tta gga tca ggg ttt aag tgt aat agt gcg gtt 1392 Ala Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta cgc aat gtc aag gca tcg gca aat agt cct tgg caa
1440 Trp Val Ala Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp
Gln 465 470 475 480 cat tgc atc gat aga tat ccg gtt aaa att gat tct
gat ttg tca aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Leu Ser Lys 485 490 495 tca aag act cat gtc caa aac ggt cgg
tcc taatttgatg tatctgagtg 1538 Ser Lys Thr His Val Gln Asn Gly Arg
Ser 500 505 ccaacgttta ctttgtcttt cctttctttt attggttatg aattagatgt
ttactaatgt 1598 tcctctcttt ttcgttataa ataaagaagt tcaattcttc
ctatagtttc aaacgcgatt 1658 ttaagcgttt ctatttaggt ttacatgaat
ttcttttaca aaccatcttt t 1709 2 506 PRT Arabidopsis thaliana 2 Met
Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10
15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys
20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser
Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala
Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro
Asn Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro
Pro His Leu Lys Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile Phe
Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Val Ala
Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln
Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140
Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145
150 155 160 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Glu
Asn Thr 165 170 175 Lys Val Asn Pro Arg Glu Ile Gly Ile Leu Val Val
Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met
Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Ile Lys Ser Phe
Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile
Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn Thr
Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Gln Gly 245 250 255 Ile
Tyr Ala Gly Glu Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265
270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Ser Gly Asp Arg
275 280 285 Arg Arg Ser Lys Tyr Lys Leu Val His Thr Val Arg Thr His
Thr Gly 290 295 300 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu
Asp Asp Glu Ser 305 310 315 320 Gly Lys Ile Gly Val Cys Leu Ser Lys
Asp Ile Thr Asn Val Ala Gly 325 330 335 Thr Thr Leu Thr Lys Asn Ile
Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys Phe
Leu Phe Phe Ala Thr Phe Val Ala Lys Lys Leu 355 360 365 Leu Lys Asp
Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 Val
Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Glu 385 390
395 400 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser
Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser
Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met
Lys Lys Gly Asn Lys 435 440 445 Ala Trp Gln Ile Ala Leu Gly Ser Gly
Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Arg Asn Val
Lys Ala Ser Ala Asn Ser Pro Trp Gln 465 470 475 480 His Cys Ile Asp
Arg Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser Lys 485 490 495 Ser Lys
Thr His Val Gln Asn Gly Arg Ser 500 505 3 1524 DNA Brassica napus
CDS (1)...(1521) 3 atg acg tcc att aac gta aag ctc ctt tac cat tac
gtc ata acc aac 48 Met Thr Ser Ile Asn Val Lys Leu Leu Tyr His Tyr
Val Ile Thr Asn 1 5 10 15 ctt ttc aac ctt tgc ttc ttt ccg tta acg
gcg atc gtc gcc gga aaa 96 Leu Phe Asn Leu Cys Phe Phe Pro Leu Thr
Ala Ile Val Ala Gly Lys 20 25 30 gcc tat cgg ctt acc ata gac gat
ctt cac cac tta tac tat tcc tat 144 Ala Tyr Arg Leu Thr Ile Asp Asp
Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 ctc caa cac aac ctc ata
acc atc gct cca ctc ttt gcc ttc acc gtt 192 Leu Gln His Asn Leu Ile
Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 ttc ggt tcg gtt
ctc tac atc gca acc cgg ccc aaa ccg gtt tac ctc 240 Phe Gly Ser Val
Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70 75 80 gtt gag
tac tca tgc tac ctt cca cca acg cat tgt aga tca agt atc 288 Val Glu
Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile 85 90 95
tcc aag gtc atg gat atc ttt tat caa gta aga aaa gct gat cct tct 336
Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro Ser 100
105 110 cgg aac ggc acg tgc gat gac tcg tcg tgg ctt gac ttc ttg agg
aag 384 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg
Lys 115 120 125 att caa gaa cgt tca ggt cta ggc gat gaa act cac ggg
ccc gag ggg 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly
Pro Glu Gly 130 135 140 ctg ctt cag gtc cct ccc cgg aag act ttt gcg
gcg gcg cgt gaa gag 480 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala
Ala Ala Arg Glu Glu 145 150 155 160 acg gag caa gtt atc att ggt gcg
cta gaa aat cta ttc aag aac acc 528 Thr Glu Gln Val Ile Ile Gly Ala
Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 aac gtt aac cct aaa gat
ata ggt ata ctt gtg gtg aac tca agc atg 576 Asn Val Asn Pro Lys Asp
Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 ttt aat cca act
cca tcg ctc tcc gcg atg gtc gtt aac act ttc aag 624 Phe Asn Pro Thr
Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 ctc cga
agc aac gta aga agc ttt aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg
Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220
gcc ggc gtt ata gcc att gat cta gca aag gac ttg ttg cat gtc cat 720
Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225
230 235 240 aaa aat acg tat gct ctt gtg gtg agc aca gag aac atc act
tat aac 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr
Tyr Asn 245 250 255 att tac gct ggt gat aat agg tcc atg atg gtt tca
aat tgc ttg ttc 816 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser
Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gct att ttg ctc tcc
aac aag cct gga gat cgt 864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser
Asn Lys Pro Gly Asp Arg 275 280 285 aga cgg tcc aag tac gag cta gtt
cac acg gtt cga acg cat acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val
His Thr Val Arg Thr His Thr Gly 290 295 300 gct gac ggc aag tct ttt
cgt tgc gtg caa caa gga gac gat gag aac 960 Ala Asp Gly Lys Ser Phe
Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 ggc aaa atc
gga gtg agt ttg tcc aag gac ata acc gat gtt gct ggt 1008 Gly Lys
Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335
cga acg gtt aag aaa aac ata gca acg ttg ggt ccg ttg att ctt ccg
1056 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu
Pro 340 345 350 tta agc gag aaa ctt ctt ttt ttc gtt acc ttc atg ggc
aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met
Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa atc aaa cat tac tac gtc
ccg gat ttc aaa ctt gct 1152 Phe Lys Asp Lys Ile Lys His Tyr Tyr
Val Pro Asp Phe Lys Leu Ala 370 375 380 att gac cat ttt tgt ata cat
gcc gga ggc aga gcc gtg att gat gtg 1200 Ile Asp His Phe Cys Ile
His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 cta gag aag
aac cta gcc cta gca ccg atc gat gta gag gca tca aga 1248 Leu Glu
Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415
tca acg tta cat aga ttt gga aac act tca tct agc tca ata tgg tat
1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp
Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa gga agg atg aag aaa
ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys
Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct tta ggg tca ggc ttt
aag tgt aac agt gca gtt 1392 Val Trp Gln Ile Ala Leu Gly Ser Gly
Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta aac aat gtc
aaa gct tcg aca aat agt cct tgg gaa 1440 Trp Val Ala Leu Asn Asn
Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 cac tgc atc
gac aga tac ccg gtc aaa att gat tct gat tca ggt aag 1488 His Cys
Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495
tca gag act cgt gtc caa aac ggt cgg tcc taa taa 1524 Ser Glu Thr
Arg Val Gln Asn Gly Arg Ser * 500 505 4 506 PRT Brassica napus 4
Met Thr Ser Ile Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 1 5
10 15 Leu Phe Asn Leu Cys Phe Phe Pro Leu Thr Ala Ile Val Ala Gly
Lys 20 25 30 Ala Tyr Arg Leu Thr Ile Asp Asp Leu His His Leu Tyr
Tyr Ser Tyr 35 40 45 Leu Gln His Asn Leu Ile Thr Ile Ala Pro Leu
Phe Ala Phe Thr Val 50 55 60 Phe Gly Ser Val Leu Tyr Ile Ala Thr
Arg Pro Lys Pro Val Tyr Leu 65 70 75 80 Val Glu Tyr Ser Cys Tyr Leu
Pro Pro Thr His Cys Arg Ser Ser Ile 85 90 95 Ser Lys Val Met Asp
Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro Ser 100 105 110 Arg Asn Gly
Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 Ile
Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135
140 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu
145 150 155 160 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe
Lys Asn Thr 165 170 175 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val
Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala
Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Val Arg Ser
Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala
Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn
Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245
250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu
Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro
Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val
Arg Thr His Thr Gly 290 295 300 Ala Asp Gly Lys Ser Phe Arg Cys Val
Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser
Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys
Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser
Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365
Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370
375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val
Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser
Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys
Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu
Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His
Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490
495 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 5 1736 DNA
Brassica napus CDS (85)...(1599) 5 tagagcgtaa cggaccacaa aagaggatcc
atacaaatac atctcatcgc ttccattact 60 attctccgac acacacactg agca atg
acg tcc att aac gta aag ctc ctt 111 Met Thr Ser Ile Asn Val Lys Leu
Leu 1 5 tac cat tac gtc ata acc aac ctt ttc aac ctt tgt ttc ttt cca
tta 159 Tyr His Tyr Val Ile Thr Asn Leu Phe Asn Leu Cys Phe Phe Pro
Leu 10 15 20 25 acg gcg atc gtc gcc gga aaa gcc tat ctt acc ata gac
gat ctt cac 207 Thr Ala Ile Val Ala Gly Lys Ala Tyr Leu Thr Ile Asp
Asp Leu His 30 35 40 cac tta tac tat tcc tat ctc caa cac aac ctc
ata acc att gct cca 255 His Leu Tyr Tyr Ser Tyr Leu Gln His Asn Leu
Ile Thr Ile Ala Pro 45 50 55 ctc ttg gcc ttc acc gtt ttc ggt tcg
gtt ctc tac atc gca acc cgg 303 Leu Leu Ala Phe Thr Val Phe Gly Ser
Val Leu Tyr Ile Ala Thr Arg 60 65 70 ccc aaa ccg gtt tac ctc gtg
gag tac tca tgc tac ctt cca cca acg 351 Pro Lys Pro Val Tyr Leu Val
Glu Tyr Ser Cys Tyr Leu Pro Pro Thr 75 80 85 cat tgt aga tca agt
atc tcc aag gtc atg gat atc ttt ttc caa gta 399 His Cys Arg Ser Ser
Ile Ser Lys Val Met Asp Ile Phe Phe Gln Val 90 95 100 105 aga aaa
gct gat cct tct cgg aac ggc acg tgc gat gac tcg tcc tgg 447 Arg Lys
Ala Asp Pro Ser Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp 110 115 120
ctt gac ttc ttg agg aag att caa gaa cgt tca ggt cta ggc gat gaa 495
Leu Asp Phe Leu Arg Lys Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu 125
130 135 acc cac ggg ccc gag ggg ctg ctt cag gtc cct ccc cgg aag act
ttt 543 Thr His Gly Pro Glu Gly Leu Leu Gln Val Pro Pro Arg Lys Thr
Phe 140 145 150 gcg cgc gcg cgt gaa gag acg gag caa gtt atc att ggt
gcg cta gaa 591 Ala Arg Ala Arg Glu Glu Thr Glu Gln Val Ile Ile Gly
Ala Leu Glu 155 160 165 aat cta ttc aag aac acc aat gtt aac cct aaa
gat ata ggt ata ctt 639 Asn Leu Phe Lys Asn Thr Asn Val Asn Pro Lys
Asp Ile Gly Ile Leu 170 175 180 185 gtg gtg aac tca agc atg ttt aat
cca act cct tcg ctc tcc gcg atg 687 Val Val Asn Ser Ser Met Phe Asn
Pro Thr Pro Ser Leu Ser Ala Met 190 195 200 gtc gtt aac act ttc aag
ctc cga agc aac gta aga agc ttt aac ctt 735 Val Val Asn Thr Phe Lys
Leu Arg Ser Asn Val Arg Ser Phe Asn Leu 205 210 215 ggt ggc atg ggt
tgt agt gcc ggc gtt ata gcc att gat cta gca aag 783 Gly Gly Met Gly
Cys Ser Ala Gly Val Ile Ala Ile Asp Leu Ala Lys 220 225 230 gac ttg
ttg cat gtc cat aaa aat acg tat gct ctt gtg gtg agc aca 831 Asp Leu
Leu His Val His Lys Asn Thr Tyr Ala Leu Val Val Ser Thr 235 240 245
gag aac atc act tat aac att tac gct ggt gat aat agg tcc atg atg 879
Glu Asn Ile Thr Tyr Asn Ile Tyr Ala Gly Asp Asn Arg Ser Met Met 250
255 260 265 gtt tca aat tgc ttg ttc cgt gtt ggt ggg gcc gct att ttg
ctc tcc 927 Val Ser Asn Cys Leu Phe Arg Val Gly Gly Ala Ala Ile Leu
Leu Ser 270 275 280 aac aag cct aga gat cgt aga cgg tcc aag tac gag
cta gtt cac acg 975 Asn Lys Pro Arg Asp Arg Arg Arg Ser Lys Tyr Glu
Leu Val His Thr 285 290 295 gtt cga acg cat acc gga gct gac gac aag
tct ttt cgt tgc gtg caa 1023 Val Arg Thr His Thr Gly Ala Asp Asp
Lys Ser Phe Arg Cys Val Gln 300 305 310 caa gga gac gat gag aac ggc
caa acc gga gtg agt ttg tcc aag gac 1071 Gln Gly Asp Asp Glu Asn
Gly Gln Thr Gly Val Ser Leu Ser Lys Asp 315 320 325 ata acc gat gtt
gct ggt cga acg gtt aag aaa aac ata gca acg ctg 1119 Ile Thr Asp
Val Ala Gly Arg Thr Val Lys Lys Asn Ile Ala Thr Leu 330 335 340 345
ggt ccg ttg att ctt ccg tta agc gag aaa ctt ctt ttt ttc gtt acc
1167 Gly Pro Leu Ile Leu Pro Leu Ser Glu Lys Leu Leu Phe Phe Val
Thr 350 355 360 ttc atg ggc aag aaa ctt ttc aaa gac gaa atc aaa cat
tat tac gtc 1215 Phe Met Gly Lys Lys Leu Phe Lys Asp Glu Ile Lys
His Tyr Tyr Val 365 370 375 ccg gac ttc aag ctt gct atc gac cat ttt
tgt ata cat gcc gga ggc 1263 Pro Asp Phe Lys Leu Ala Ile Asp His
Phe Cys Ile His Ala Gly Gly 380 385 390 aaa gcc gtg att gat gtg cta
gag aag aac cta ggc cta gca ccg atc 1311 Lys Ala Val Ile Asp Val
Leu Glu Lys Asn Leu Gly Leu Ala Pro Ile 395 400 405 gat gta gag gca
tca aga tca acg tta cat aga ttt gga aac act tca 1359 Asp Val Glu
Ala Ser Arg Ser Thr Leu His Arg Phe Gly Asn Thr Ser 410 415 420 425
tct agc tca ata tgg tat gag ttg gca tac ata gaa ccc aaa gga agg
1407 Ser Ser Ser Ile Trp Tyr Glu Leu Ala Tyr Ile Glu Pro Lys Gly
Arg 430 435 440 atg aag aaa ggt aat aaa gtt tgg cag att gct tta ggg
tca ggc ttt 1455 Met Lys Lys Gly Asn Lys Val Trp Gln Ile Ala Leu
Gly Ser Gly Phe 445 450 455 aag tgt aac agt gca gtt tgg gtg gct cta
aac aat gtc aaa gct tca 1503 Lys Cys Asn Ser Ala Val Trp Val Ala
Leu Asn Asn Val Lys Ala Ser 460 465 470 aca aat agt cct tgg gaa cac
tgc atc gac aga tac ccg gtt aaa att 1551 Thr Asn Ser Pro Trp Glu
His Cys Ile Asp Arg Tyr Pro Val Lys Ile 475 480 485 gat tct gat tca
ggt aag tca gag act cgt gtc cca aac ggt cgg tcc 1599 Asp Ser Asp
Ser Gly Lys Ser Glu Thr Arg Val Pro Asn Gly Arg Ser 490 495 500 505
taataaatga tgtttgctct ctttcgtttc tttttattgg ttataataat ttgatggcca
1659 cgatgtttct cttgtttgtt atgaataaag aatcccacgg tgttctagta
aaaaaaaaaa 1719 aaaaaaaaaa aaaaaaa 1736 6 505 PRT Brassica napus 6
Met Thr Ser Ile Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 1 5
10 15 Leu Phe Asn Leu Cys Phe Phe Pro Leu Thr Ala Ile Val Ala Gly
Lys 20 25 30 Ala Tyr Leu Thr Ile Asp Asp Leu His His Leu Tyr Tyr
Ser Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Ile Ala Pro Leu Leu
Ala Phe Thr Val Phe 50 55 60 Gly Ser Val Leu Tyr Ile Ala Thr Arg
Pro Lys Pro Val Tyr Leu Val 65 70 75 80 Glu Tyr Ser Cys Tyr Leu Pro
Pro Thr His Cys Arg Ser Ser Ile Ser 85 90 95 Lys Val Met Asp Ile
Phe Phe Gln Val Arg Lys Ala Asp Pro Ser Arg 100 105 110 Asn Gly Thr
Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys Ile 115 120 125 Gln
Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly Leu 130 135
140 Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Arg Ala Arg Glu Glu Thr
145 150 155 160 Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys
Asn Thr Asn 165 170 175 Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val
Asn Ser Ser Met Phe 180 185 190 Asn Pro Thr Pro Ser Leu Ser Ala Met
Val Val Asn Thr Phe Lys Leu 195 200 205 Arg Ser Asn Val Arg Ser Phe
Asn Leu Gly Gly Met Gly Cys Ser Ala 210 215 220 Gly Val Ile Ala Ile
Asp Leu Ala Lys Asp Leu Leu His Val His Lys 225 230 235 240 Asn Thr
Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn Ile 245 250 255
Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe Arg 260
265 270 Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Arg Asp Arg
Arg 275 280 285 Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His
Thr Gly Ala 290 295 300 Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly
Asp Asp Glu Asn Gly 305 310 315 320 Gln Thr Gly Val Ser Leu Ser Lys
Asp Ile Thr Asp Val Ala Gly Arg 325 330 335 Thr Val Lys Lys Asn Ile
Ala Thr Leu Gly Pro Leu Ile Leu Pro Leu 340 345 350 Ser Glu Lys Leu
Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu Phe 355 360 365 Lys Asp
Glu Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala Ile 370 375 380
Asp His Phe Cys Ile His Ala Gly Gly Lys Ala Val Ile Asp Val Leu 385
390 395 400 Glu Lys Asn Leu Gly Leu Ala Pro Ile Asp Val Glu Ala Ser
Arg Ser 405 410 415 Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser
Ile Trp Tyr Glu 420 425 430 Leu Ala Tyr Ile Glu Pro Lys Gly Arg Met
Lys Lys Gly Asn Lys Val 435 440 445 Trp Gln Ile Ala Leu Gly Ser Gly
Phe Lys Cys Asn Ser Ala Val Trp 450 455 460 Val Ala Leu Asn Asn Val
Lys Ala Ser Thr Asn Ser Pro Trp Glu His 465 470 475 480 Cys Ile Asp
Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys Ser 485 490 495 Glu
Thr Arg Val Pro Asn Gly Arg Ser 500 505 7 1521 DNA Artificial
Sequence 5' 342 bp from A. thaliana FAE1 (SEQ ID NO1) and 3' 1179
bp from B. napus elongase KCS (SEQ ID NO3); designated At114 7 atg
acg tcc gtt aac gtt aag ctc ctt tac cgt tat gtc tta acc aac 48 Met
Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10
15 ttt ttc aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa
96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys
20 25 30 gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc
tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser
Tyr Leu 35 40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct
ttc act gtt ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala
Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc
aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro
Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt ccg cca
ccg cat ctc aaa gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro
Pro His Leu Lys Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc
tac caa ata aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe
Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac ggc acg
tgt gat gat tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr
Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa
gaa cgt tca ggt cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln
Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140
ctg ctt cag gtc cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480
Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145
150 155 160 acg gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag
aac acc 528 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys
Asn Thr 165 170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg
aac tca agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val
Asn Ser Ser Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg
gtc gtt aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met
Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt
aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe
Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att
gat cta gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile
Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg
tat gct ctt gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr
Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att
tac gct ggt gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile
Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265
270 cgt gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt
864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg
275 280 285 aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat
acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His
Thr Gly 290 295 300 gct gac ggc aag tct ttt cgt tgc gtg caa caa gga
gac gat gag aac 960 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly
Asp Asp Glu Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag
gac ata acc gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser
Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac
ata gca acg ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys
Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag
aaa ctt ctt ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser
Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365
ttc aaa gat aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct
1152 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu
Ala 370 375 380 att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg
att gat gtg 1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala
Val Ile Asp Val 385 390 395 400 cta gag aag aac cta gcc cta gca ccg
atc gat gta gag gca tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala
Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt
gga aac act tca tct agc tca ata tgg tat 1296 Ser Thr Leu His Arg
Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca
tac ata gaa rca aaa gga agg atg aag aaa ggt aat aaa 1344 Glu Leu
Ala Tyr Ile Glu Xaa Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445
gtt tgg cag att gct tta ggg tca ggc ttt aag tgt aac agt gca gtt
1392 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala
Val 450 455 460 tgg gtg gct cta aac aat gtc aaa gct tcg aca aat agt
cct tgg gaa 1440 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn
Ser Pro Trp Glu 465 470 475 480 cac tgc atc gac aga tac ccg gtc aaa
att gat tct gat tca ggt aag 1488 His Cys Ile Asp Arg Tyr Pro Val
Lys Ile Asp Ser Asp Ser
Gly Lys 485 490 495 tca gag act cgt gtc cca aac ggt cgg tcc taa
1521 Ser Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 8 506 PRT
Artificial Sequence VARIANT (0)...(0) Xaa = Ala or Thr 8 Met Thr
Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15
Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20
25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr
Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe
Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn
Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro
His Leu Lys Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr
Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Gly Thr Cys
Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu
Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 Leu
Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150
155 160 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn
Thr 165 170 175 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn
Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val
Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Val Arg Ser Phe Asn
Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp
Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn Thr Tyr
Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 Ile Tyr
Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270
Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275
280 285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr
Gly 290 295 300 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp
Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp
Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys Lys Asn Ile Ala
Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys Leu Leu
Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 Phe Lys Asp Lys
Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 Ile Asp
His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395
400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg
405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile
Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Xaa Lys Gly Arg Met Lys
Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe
Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Asn Asn Val Lys
Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His Cys Ile Asp Arg
Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 Ser Glu Thr
Arg Val Pro Asn Gly Arg Ser 500 505 9 1518 DNA Artificial Sequence
5' 222 bp from A. thaliana FAE1 (SEQ ID NO1) and 3' 1296 bp from B.
napus elongase KCS (SEQ ID NO3); designated At74 9 atg acg tcc gtt
aac gtt aag ctc ctt tac cgt tac gtc tta acc aac 48 Met Thr Ser Val
Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc
aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe
Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30
gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144
Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35
40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt
ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val
Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aaa ccg gtt
tac ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Lys Pro Val
Tyr Leu Val 65 70 75 80 gag tac tca tgc tac ctt cca cca acg cat tgt
aga tca agt atc tcc 288 Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys
Arg Ser Ser Ile Ser 85 90 95 aag gtc atg gat atc ttt tat caa gta
aga aaa gct gat cct tct cgg 336 Lys Val Met Asp Ile Phe Tyr Gln Val
Arg Lys Ala Asp Pro Ser Arg 100 105 110 aac ggc acg tgc gat gac tcg
tcg tgg ctt gac ttc ttg agg aag att 384 Asn Gly Thr Cys Asp Asp Ser
Ser Trp Leu Asp Phe Leu Arg Lys Ile 115 120 125 caa gaa cgt tca ggt
cta ggc gat gaa act cac ggg ccc gag ggg ctg 432 Gln Glu Arg Ser Gly
Leu Gly Asp Glu Thr His Gly Pro Glu Gly Leu 130 135 140 ctt cag gtc
cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag acg 480 Leu Gln Val
Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu Thr 145 150 155 160
gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc aac 528
Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr Asn 165
170 175 gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc atg
ttt 576 Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met
Phe 180 185 190 aat cca act cca tcg ctc tcc gcg atg gtc gtt aac act
ttc aag ctc 624 Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr
Phe Lys Leu 195 200 205 cga agc aac gta aga agc ttt aac ctt ggt ggc
atg ggt tgt agt gcc 672 Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly
Met Gly Cys Ser Ala 210 215 220 ggc gtt ata gcc att gat cta gca aag
gac ttg ttg cat gtc cat aaa 720 Gly Val Ile Ala Ile Asp Leu Ala Lys
Asp Leu Leu His Val His Lys 225 230 235 240 aat acg tat gct ctt gtg
gtg agc aca gag aac atc act tat aac att 768 Asn Thr Tyr Ala Leu Val
Val Ser Thr Glu Asn Ile Thr Tyr Asn Ile 245 250 255 tac gct ggt gat
aat agg tcc atg atg gtt tca aat tgc ttg ttc cgt 816 Tyr Ala Gly Asp
Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe Arg 260 265 270 gtt ggt
ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt aga 864 Val Gly
Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg Arg 275 280 285
cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga gct 912
Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly Ala 290
295 300 gac ggc aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac
ggc 960 Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn
Gly 305 310 315 320 aaa atc gga gtg agt ttg tcc aag gac ata acc gat
gtt gct ggt cga 1008 Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr
Asp Val Ala Gly Arg 325 330 335 acg gtt aag aaa aac ata gca acg ttg
ggt ccg ttg att ctt ccg tta 1056 Thr Val Lys Lys Asn Ile Ala Thr
Leu Gly Pro Leu Ile Leu Pro Leu 340 345 350 agc gag aaa ctt ctt ttt
ttc gtt acc ttc atg ggc aag aaa ctt ttc 1104 Ser Glu Lys Leu Leu
Phe Phe Val Thr Phe Met Gly Lys Lys Leu Phe 355 360 365 aaa gat aaa
atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct att 1152 Lys Asp
Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala Ile 370 375 380
gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg cta
1200 Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val
Leu 385 390 395 400 gag aag aac cta gcc cta gca ccg atc gat gta gag
gca tca aga tca 1248 Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val
Glu Ala Ser Arg Ser 405 410 415 acg tta cat aga ttt gga aac act tca
tct agc tca ata tgg tat gag 1296 Thr Leu His Arg Phe Gly Asn Thr
Ser Ser Ser Ser Ile Trp Tyr Glu 420 425 430 ttg gca tac ata gaa gca
aaa gga agg atg aag aaa ggt aat aaa gtt 1344 Leu Ala Tyr Ile Glu
Ala Lys Gly Arg Met Lys Lys Gly Asn Lys Val 435 440 445 tgg cag att
gct tta ggg tca ggc ttt aag tgt aac agt gca gtt tgg 1392 Trp Gln
Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val Trp 450 455 460
gtg gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa cac
1440 Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu
His 465 470 475 480 tgc atc gac aga tac ccg gtc aaa att gat tct gat
tca ggt aag tca 1488 Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser
Asp Ser Gly Lys Ser 485 490 495 gag act cgt gtc caa aac ggt cgg tcc
taa 1518 Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 10 505 PRT
Artificial Sequence 5' 74 amino acids from A. thaliana FAE1 (SEQ ID
NO2) and 3' 431 amino acids from B. napus elongase KCS (SEQ ID
NO4); designated At74 10 Met Thr Ser Val Asn Val Lys Leu Leu Tyr
Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro
Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile
Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn Leu
Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly Leu
Val Leu Tyr Ile Val Thr Arg Pro Lys Pro Val Tyr Leu Val 65 70 75 80
Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile Ser 85
90 95 Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro Ser
Arg 100 105 110 Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu
Arg Lys Ile 115 120 125 Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His
Gly Pro Glu Gly Leu 130 135 140 Leu Gln Val Pro Pro Arg Lys Thr Phe
Ala Ala Ala Arg Glu Glu Thr 145 150 155 160 Glu Gln Val Ile Ile Gly
Ala Leu Glu Asn Leu Phe Lys Asn Thr Asn 165 170 175 Val Asn Pro Lys
Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met Phe 180 185 190 Asn Pro
Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys Leu 195 200 205
Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser Ala 210
215 220 Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His
Lys 225 230 235 240 Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile
Thr Tyr Asn Ile 245 250 255 Tyr Ala Gly Asp Asn Arg Ser Met Met Val
Ser Asn Cys Leu Phe Arg 260 265 270 Val Gly Gly Ala Ala Ile Leu Leu
Ser Asn Lys Pro Gly Asp Arg Arg 275 280 285 Arg Ser Lys Tyr Glu Leu
Val His Thr Val Arg Thr His Thr Gly Ala 290 295 300 Asp Gly Lys Ser
Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn Gly 305 310 315 320 Lys
Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly Arg 325 330
335 Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro Leu
340 345 350 Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys
Leu Phe 355 360 365 Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe
Lys Leu Ala Ile 370 375 380 Asp His Phe Cys Ile His Ala Gly Gly Arg
Ala Val Ile Asp Val Leu 385 390 395 400 Glu Lys Asn Leu Ala Leu Ala
Pro Ile Asp Val Glu Ala Ser Arg Ser 405 410 415 Thr Leu His Arg Phe
Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr Glu 420 425 430 Leu Ala Tyr
Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys Val 435 440 445 Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val Trp 450 455
460 Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu His
465 470 475 480 Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser
Gly Lys Ser 485 490 495 Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505
11 1521 DNA Artificial Sequence 5' 342 bp from A. thaliana FAE1
(SEQ ID NO1) and 3' 1179 bp from B. napus elongase KCS (SEQ ID NO3)
having mutations at positions 271, 272 and 275; designated At114
L91C K92R 11 atg acg tcc gtt aac gtt aag ctc ctt tac cgt tat gtc
tta acc aac 48 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val
Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt ttg ttc ccg tta acg gcg
ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala
Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg ctt acc ata aac gat ctc
cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp Leu
His Asn Phe Leu Ser Tyr Leu 35 40 45 caa cac aac ctt ata aca gta
act tta ctc ttt gct ttc act gtt ttc 192 Gln His Asn Leu Ile Thr Val
Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc tac
atc gta acc cga ccc aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu Tyr
Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac tcg
tgt tac ctt ccg cca ccg cat tgc aga gtt agt gtc tct 288 Asp Tyr Ser
Cys Tyr Leu Pro Pro Pro His Cys Arg Val Ser Val Ser 85 90 95 aaa
gtc atg gat att ttc tac caa ata aga aaa gct gat act tct tca 336 Lys
Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105
110 cgg aac ggc acg tgt gat aat tcg tcg tgg ctt gac ttc ttg agg aag
384 Arg Asn Gly Thr Cys Asp Asn Ser Ser Trp Leu Asp Phe Leu Arg Lys
115 120 125 att caa gaa cgt tca ggt cta ggc gat gaa act cac ggg ccc
gag ggg 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro
Glu Gly 130 135 140 ctg ctt cag gtc cct ccc cgg aag act ttt gcg gcg
gcg cgt gaa gag 480 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala
Ala Arg Glu Glu 145 150 155 160 acg gag caa gtt atc att ggt gcg cta
gaa aat cta ttc aag aac acc 528 Thr Glu Gln Val Ile Ile Gly Ala Leu
Glu Asn Leu Phe Lys Asn Thr 165 170 175 aac gtt aac cct aaa gat ata
ggt ata ctt gtg gtg aac tca agc atg 576 Asn Val Asn Pro Lys Asp Ile
Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 ttt aat cca act cca
tcg ctc tcc gcg atg gtc gtt aac act ttc aag 624 Phe Asn Pro Thr Pro
Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc
aac gta aga agc ttt aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg Ser
Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gcc
ggc gtt ata gcc att gat cta gca aag gac ttg ttg cat gtc cat 720 Ala
Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230
235 240 aaa aat acg tat gct ctt gtg gtg agc aca gag aac atc act tat
aac 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr
Asn 245 250 255 att tac gct ggt gat aat agg tcc atg atg gtt tca aat
tgc ttg ttc 816 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn
Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gct att ttg ctc tcc aac
aag cct gga gat cgt 864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn
Lys Pro Gly Asp Arg 275 280 285 aga cgg tcc aag tac gag cta gtt cac
acg gtt cga acg cat acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val His
Thr Val Arg Thr His Thr Gly 290 295
300 gct gac ggc aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac
960 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn
305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc gat
gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr
Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg ttg
ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala Thr
Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt ttt
ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu
Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa
atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys Asp
Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 att
gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg 1200
Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385
390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta gag gca
tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu
Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act tca tct
agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser
Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa
gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala
Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct
tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp Gln Ile
Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg
gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa 1440 Trp
Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470
475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct gat tca ggt
aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser
Gly Lys 485 490 495 tca gag act cgt gtc cca aac ggt cgg tcc taa
1521 Ser Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 12 506 PRT
Artificial Sequence 5' 114 amino acids from A. thaliana FAE1 (SEQ
ID NO2) and 3' 392 amino acids from B. napus elongase KCS (SEQ ID
NO4) having mutations at residues 91 and 92; designated At114 L91C
K92R 12 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr
Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu
Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn
Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu
Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val
Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr
Leu Pro Pro Pro His Cys Arg Val Ser Val Ser 85 90 95 Lys Val Met
Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg
Asn Gly Thr Cys Asp Asn Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120
125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly
130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg
Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn
Leu Phe Lys Asn Thr 165 170 175 Asn Val Asn Pro Lys Asp Ile Gly Ile
Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu
Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Val
Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val
Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240
Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245
250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu
Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro
Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val
Arg Thr His Thr Gly 290 295 300 Ala Asp Gly Lys Ser Phe Arg Cys Val
Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser
Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys
Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser
Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365
Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370
375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val
Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser
Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys
Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu
Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His
Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490
495 Ser Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 13 1521 DNA
Artificial Sequence 5' 342 bp from A. thaliana FAE1 (SEQ ID NO1)
and 3' 1179 bp from B. napus elongase KCS (SEQ ID NO3), having a
mutation at position 275; designated At114 K92R 13 atg acg tcc gtt
aac gtt aag ctc ctt tac cgt tat gtc tta acc aac 48 Met Thr Ser Val
Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc
aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe
Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30
gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144
Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35
40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt
ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val
Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg gtt
tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val
Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt ccg cca ccg cat ctc
aga gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu
Arg Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc tac caa ata
aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln Ile
Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac ggc acg tgt gat gat
tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp Asp
Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt tca
ggt cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg Ser
Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt cag
gtc cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu Gln
Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160
acg gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc 528
Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165
170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc
atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser
Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt aac
act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn
Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt aac ctt ggt
ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly
Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att gat cta gca
aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala
Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg tat gct ctt
gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala Leu
Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct ggt
gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala Gly
Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt
ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg Val
Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285
aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga 912
Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290
295 300 gct gac ggc aag tct ttt cgt tgc gtg caa caa gga gac gat gag
aac 960 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu
Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc
gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile
Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg
ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala
Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt
ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu
Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat
aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys
Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380
att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg
1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta
gag gca tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act
tca tct agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa
gca aaa gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag
att gct tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa
1440 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp
Glu 465 470 475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct
gat tca ggt aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Ser Gly Lys 485 490 495 tca gag act cgt gtc cca aac ggt cgg
tcc taa 1521 Ser Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 14 506
PRT Artificial Sequence 5' 114 amino acids from A. thaliana FAE1
(SEQ ID NO2) and 3' 392 amino acids from B. napus elongase KCS (SEQ
ID NO4), having a mutation at position 92; designated At114 K92R 14
Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5
10 15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly
Lys 20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu
Ser Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe
Ala Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg
Pro Asn Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro
Pro Pro His Leu Arg Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile
Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Gly
Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 Ile
Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135
140 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu
145 150 155 160 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe
Lys Asn Thr 165 170 175 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val
Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala
Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Val Arg Ser
Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala
Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn
Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255
Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260
265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp
Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr
His Thr Gly 290 295 300 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln
Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser Leu Ser
Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys Lys Asn
Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys
Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 Phe Lys
Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380
Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385
390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala
Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg
Met Lys Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu Gly Ser
Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Asn Asn
Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His Cys Ile
Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 Ser
Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 15 1521 DNA Artificial
Sequence 5' 342 bp from A. thaliana FAE1 (SEQ ID NO1) and 3' 1179
bp from B. napus elongase KCS (SEQ ID NO3), having a mutation at
position 920; designated At114 G307D; hypothetical 15 atg acg tcc
gtt aac gtt aag ctc ctt tac cgt tat gtc tta acc aac 48 Met Thr Ser
Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt
ttc aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe
Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25
30 gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc
144 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu
35 40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act
gtt ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr
Val Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg
gtt tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro
Val Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac
ctt ccg cca ccg cat ctc aaa gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr
Leu Pro Pro Pro His Leu Lys Val Ser Val Ser 85 90 95 aaa gtc atg
gat att ttc tac caa ata aga aaa gct gat act tct tca 336 Lys Val Met
Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg
aac ggc acg tgt gat gat tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg
Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120
125 att caa gaa cgt tca ggt cta ggc gat gaa act cac ggg ccc gag ggg
432 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly
130 135 140 ctg ctt cag gtc cct ccc cgg aag act ttt gcg gcg gcg cgt
gaa gag 480 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg
Glu Glu 145 150 155 160 acg gag caa gtt atc att ggt gcg cta gaa aat
cta ttc aag aac acc 528 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn
Leu Phe Lys Asn Thr 165 170 175 aac gtt aac cct aaa gat ata ggt ata
ctt gtg gtg aac tca agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile
Leu Val Val Asn Ser Ser Met 180 185 190 ttt aat cca act cca tcg ctc
tcc gcg atg gtc gtt aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu
Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta
aga agc ttt aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val
Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt
ata gcc att gat cta gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val
Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240
aaa aat acg tat gct ctt gtg gtg agc aca gag aac atc act tat aac 768
Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245
250 255 att tac gct ggt gat aat agg tcc atg atg gtt tca aat tgc ttg
ttc 816 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu
Phe 260 265 270 cgt gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct
gga gat cgt 864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro
Gly Asp Arg 275 280 285 aga cgg tcc aag tac gag cta gtt cac acg gtt
cga acg cat acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val
Arg Thr His Thr Gly 290 295 300 gct gac gac aag tct ttt cgt tgc gtg
caa caa gga gac gat gag aac 960 Ala Asp Asp Lys Ser Phe Arg Cys Val
Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 ggc aaa atc gga gtg agt
ttg tcc aag gac ata acc gat gtt gct ggt 1008 Gly Lys Ile Gly Val
Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 cga acg gtt
aag aaa aac ata gca acg ttg ggt ccg ttg att ctt ccg 1056 Arg Thr
Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350
tta agc gag aaa ctt ctt ttt ttc gtt acc ttc atg ggc aag aaa ctt
1104 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys
Leu 355 360 365 ttc aaa gat aaa atc aaa cat tac tac gtc ccg gat ttc
aaa ctt gct 1152 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp
Phe Lys Leu Ala 370 375 380 att gac cat ttt tgt ata cat gcc gga ggc
aga gcc gtg att gat gtg 1200 Ile Asp His Phe Cys Ile His Ala Gly
Gly Arg Ala Val Ile Asp Val 385 390 395 400 cta gag aag aac cta gcc
cta gca ccg atc gat gta gag gca tca aga 1248 Leu Glu Lys Asn Leu
Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 tca acg tta
cat aga ttt gga aac act tca tct agc tca ata tgg tat 1296 Ser Thr
Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430
gag ttg gca tac ata gaa gca aaa gga agg atg aag aaa ggt aat aaa
1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn
Lys 435 440 445 gtt tgg cag att gct tta ggg tca ggc ttt aag tgt aac
agt gca gtt 1392 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys
Asn Ser Ala Val 450 455 460 tgg gtg gct cta aac aat gtc aaa gct tcg
aca aat agt cct tgg gaa 1440 Trp Val Ala Leu Asn Asn Val Lys Ala
Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 cac tgc atc gac aga tac
ccg gtc aaa att gat tct gat tca ggt aag 1488 His Cys Ile Asp Arg
Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 tca gag act
cgt gtc caa aac ggt cgg tcc taa 1521 Ser Glu Thr Arg Val Gln Asn
Gly Arg Ser 500 505 16 506 PRT Artificial Sequence 5' 114 amino
acids from A. thaliana FAE1 (SEQ ID NO2) and 3' 392 amino acids
from B. napus elongase KCS (SEQ ID NO4) having mutation at residue
307; designated At114 G307D; hypothetical 16 Met Thr Ser Val Asn
Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn
Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala
Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40
45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe
50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr
Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Lys
Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg
Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Gly Thr Cys Asp Asp Ser
Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly
Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val
Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr
Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170
175 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met
180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr
Phe Lys 195 200 205 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly
Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys
Asp Leu Leu His Val His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val
Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp
Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly
Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg
Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295
300 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn
305 310 315 320 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp
Val Ala Gly 325 330 335 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly
Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val
Thr Phe Met Gly Lys Lys Leu 355 360 365 Phe Lys Asp Lys Ile Lys His
Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 Ile Asp His Phe Cys
Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 Leu Glu
Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415
Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420
425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn
Lys 435 440 445 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn
Ser Ala Val 450 455 460 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr
Asn Ser Pro Trp Glu 465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val
Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 Ser Glu Thr Arg Val Gln
Asn Gly Arg Ser 500 505 17 1518 DNA Artificial Sequence 5' 222 bp
from A. thaliana FAE1 (SEQ ID NO1) and 3' 1296 bp from B. napus
elongase KCS (SEQ ID NO3) having a mutation at position 917;
designated At74 G306D; hypothetical 17 atg acg tcc gtt aac gtt aag
ctc ctt tac cgt tac gtc tta acc aac 48 Met Thr Ser Val Asn Val Lys
Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt
ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys
Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg
ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg
Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 caa
cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt ttc 192 Gln
His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55
60 ggt ttg gtt ctc tac atc gta acc cga ccc aaa ccg gtt tac ctc gtt
240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Lys Pro Val Tyr Leu Val
65 70 75 80 gag tac tca tgc tac ctt cca cca acg cat tgt aga tca agt
atc tcc 288 Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser
Ile Ser 85 90 95 aag gtc atg gat atc ttt tat caa gta aga aaa gct
gat cct tct cgg 336 Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala
Asp Pro Ser Arg 100 105 110 aac ggc acg tgc gat gac tcg tcg tgg ctt
gac ttc ttg agg aag att 384 Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu
Asp Phe Leu Arg Lys Ile 115 120 125 caa gaa cgt tca ggt cta ggc gat
gaa act cac ggg ccc gag ggg ctg 432 Gln Glu Arg Ser Gly Leu Gly Asp
Glu Thr His Gly Pro Glu Gly Leu 130 135 140 ctt cag gtc cct ccc cgg
aag act ttt gcg gcg gcg cgt gaa gag acg 480 Leu Gln Val Pro Pro Arg
Lys Thr Phe Ala Ala Ala Arg Glu Glu Thr 145 150 155 160 gag caa gtt
atc att ggt gcg cta gaa aat cta ttc aag aac acc aac 528 Glu Gln Val
Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr Asn 165 170 175 gtt
aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc atg ttt 576 Val
Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met Phe 180 185
190 aat cca act cca tcg ctc tcc gcg atg gtc gtt aac act ttc aag ctc
624 Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys Leu
195 200 205 cga agc aac gta aga agc ttt aac ctt ggt ggc atg ggt tgt
agt gcc 672 Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys
Ser Ala 210 215 220 ggc gtt ata gcc att gat cta gca aag gac ttg ttg
cat gtc cat aaa 720 Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu
His Val His Lys 225 230 235 240 aat acg tat gct ctt gtg gtg agc aca
gag aac atc act tat aac att 768 Asn Thr Tyr Ala Leu Val Val Ser Thr
Glu Asn Ile Thr Tyr Asn Ile 245 250 255 tac gct ggt gat aat agg tcc
atg atg gtt tca aat tgc ttg ttc cgt 816 Tyr Ala Gly Asp Asn Arg Ser
Met Met Val Ser Asn Cys Leu Phe Arg 260 265 270 gtt ggt ggg gcc gct
att ttg ctc tcc aac aag cct gga gat cgt aga 864 Val Gly Gly Ala Ala
Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg Arg 275 280 285 cgg tcc aag
tac gag cta gtt cac acg gtt cga acg cat acc gga gct 912 Arg Ser Lys
Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly Ala 290 295 300 gac
gac aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac ggc 960 Asp
Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn Gly 305 310
315 320 aaa atc gga gtg agt ttg tcc aag gac ata acc gat gtt gct ggt
cga 1008 Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala
Gly Arg 325 330 335 acg gtt aag aaa aac ata gca acg ttg ggt ccg ttg
att ctt ccg tta 1056 Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro
Leu Ile Leu Pro Leu 340 345 350 agc gag aaa ctt ctt ttt ttc gtt acc
ttc atg ggc aag aaa ctt ttc 1104 Ser Glu Lys Leu Leu Phe Phe Val
Thr Phe Met Gly Lys Lys Leu Phe 355 360 365 aaa gat aaa atc aaa cat
tac tac gtc ccg gat ttc aaa ctt gct att 1152 Lys Asp Lys Ile Lys
His Tyr Tyr Val Pro Asp Phe Lys Leu Ala Ile 370 375 380 gac cat ttt
tgt ata cat gcc gga ggc aga gcc gtg att gat gtg cta 1200 Asp His
Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val Leu 385 390 395
400 gag aag aac cta gcc cta gca ccg atc gat gta gag gca tca aga tca
1248 Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg
Ser 405 410 415 acg tta cat aga ttt gga aac act tca tct agc tca ata
tgg tat gag 1296 Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser
Ile Trp Tyr Glu 420 425 430 ttg gca tac ata gaa gca aaa gga agg atg
aag aaa ggt aat aaa gtt 1344 Leu Ala Tyr Ile Glu Ala Lys Gly Arg
Met Lys Lys Gly Asn Lys Val 435 440 445 tgg cag att gct tta ggg tca
ggc ttt aag tgt aac agt gca gtt tgg 1392 Trp Gln Ile Ala Leu Gly
Ser Gly Phe Lys Cys Asn Ser Ala Val Trp 450 455 460 gtg gct cta aac
aat gtc aaa gct tcg aca aat agt cct tgg gaa cac 1440 Val Ala Leu
Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu His 465 470 475 480
tgc atc gac aga tac ccg gtc aaa att gat tct gat tca ggt aag tca
1488 Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys
Ser 485 490 495 gag act cgt gtc caa aac ggt cgg tcc taa 1518 Glu
Thr Arg Val Gln Asn Gly Arg Ser 500 505 18 505 PRT Artificial
Sequence 5' 74 amino acids from A. thaliana FAE1 (SEQ ID NO2) and
3' 431 amino acids from B. napus elongase KCS (SEQ ID NO4) having a
mutation at residue 306; designated At74 G306D; hypothetical 18 Met
Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10
15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys
20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser
Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala
Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro
Lys Pro Val Tyr Leu Val 65 70 75 80 Glu Tyr Ser Cys Tyr Leu Pro Pro
Thr His Cys Arg Ser Ser Ile Ser 85 90 95 Lys Val Met Asp Ile Phe
Tyr Gln Val Arg Lys Ala Asp Pro Ser Arg 100 105 110 Asn Gly Thr Cys
Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys Ile 115 120 125 Gln Glu
Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly Leu 130 135 140
Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu Thr 145
150 155 160 Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn
Thr Asn 165 170 175 Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn
Ser Ser Met Phe 180 185 190 Asn Pro Thr Pro Ser Leu Ser Ala Met Val
Val Asn Thr Phe Lys Leu 195 200 205 Arg Ser Asn Val Arg Ser Phe Asn
Leu Gly Gly Met Gly Cys Ser Ala 210 215 220 Gly Val Ile Ala Ile Asp
Leu Ala Lys Asp Leu Leu His Val His Lys 225 230 235 240 Asn Thr Tyr
Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn Ile 245 250 255 Tyr
Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe Arg 260 265
270 Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg Arg
275 280 285 Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr
Gly Ala 290 295 300 Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp
Asp Glu Asn Gly 305 310 315
320 Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly Arg
325 330 335 Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu
Pro Leu 340 345 350 Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly
Lys Lys Leu Phe 355 360 365 Lys Asp Lys Ile Lys His Tyr Tyr Val Pro
Asp Phe Lys Leu Ala Ile 370 375 380 Asp His Phe Cys Ile His Ala Gly
Gly Arg Ala Val Ile Asp Val Leu 385 390 395 400 Glu Lys Asn Leu Ala
Leu Ala Pro Ile Asp Val Glu Ala Ser Arg Ser 405 410 415 Thr Leu His
Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr Glu 420 425 430 Leu
Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys Val 435 440
445 Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val Trp
450 455 460 Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp
Glu His 465 470 475 480 Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser
Asp Ser Gly Lys Ser 485 490 495 Glu Thr Arg Val Gln Asn Gly Arg Ser
500 505 19 1521 DNA Artificial Sequence 5' 342 bp from A. thaliana
FAE1 (SEQ ID NO1) and 3' 1179 bp from B. napus elongase KCS (SEQ ID
NO3) having mutations at positions 271, 272, 275 and 920;
designated At114 L91C K92R G307D; hypothetical 19 atg acg tcc gtt
aac gtt aag ctc ctt tac cgt tat gtc tta acc aac 48 Met Thr Ser Val
Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc
aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe
Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30
gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144
Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35
40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt
ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val
Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg gtt
tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val
Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt ccg cca ccg cat tgc
aga gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Cys
Arg Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc tac caa ata
aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln Ile
Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac ggc acg tgt gat aat
tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp Asn
Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt tca
ggt cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg Ser
Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt cag
gtc cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu Gln
Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160
acg gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc 528
Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165
170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc
atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser
Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt aac
act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn
Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt aac ctt ggt
ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly
Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att gat cta gca
aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala
Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg tat gct ctt
gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala Leu
Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct ggt
gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala Gly
Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt
ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg Val
Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285
aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga 912
Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290
295 300 gct gac gac aag tct ttt cgt tgc gtg caa caa gga gac gat gag
aac 960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu
Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc
gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile
Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg
ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala
Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt
ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu
Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat
aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys
Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380
att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg
1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta
gag gca tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act
tca tct agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa
gca aaa gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag
att gct tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa
1440 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp
Glu 465 470 475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct
gat tca ggt aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Ser Gly Lys 485 490 495 tca gag act cgt gtc caa aac ggt cgg
tcc taa 1521 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 20 506
PRT Artificial Sequence 5' 114 amino acids from A. thaliana FAE1
(SEQ ID NO2) and 3' 392 amino acids from B. napus elongase KCS (SEQ
ID NO4) having mutations at positions 91, 92 and 307; designated
At114 L91C K92R G307D; hypothetical 20 Met Thr Ser Val Asn Val Lys
Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys
Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg
Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln
His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55
60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val
65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Cys Arg Val Ser
Val Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala
Asp Thr Ser Ser 100 105 110 Arg Asn Gly Thr Cys Asp Asn Ser Ser Trp
Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly
Asp Glu Thr His Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro
Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln
Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn
Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185
190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys
195 200 205 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly
Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu
Leu His Val His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser
Thr Glu Asn Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg
Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala
Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser
Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala
Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310
315 320 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala
Gly 325 330 335 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu
Ile Leu Pro 340 345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe
Met Gly Lys Lys Leu 355 360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr
Val Pro Asp Phe Lys Leu Ala 370 375 380 Ile Asp His Phe Cys Ile His
Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 Leu Glu Lys Asn
Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr
Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430
Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435
440 445 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala
Val 450 455 460 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser
Pro Trp Glu 465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile
Asp Ser Asp Ser Gly Lys 485 490 495 Ser Glu Thr Arg Val Gln Asn Gly
Arg Ser 500 505 21 1521 DNA Artificial Sequence 5' 342 bp from A.
thaliana FAE1 (SEQ ID NO1) and 3' 1179 bp from B. napus elongase
KCS (SEQ ID NO3) having mutations at positions 275 and 920;
designated At114 K92R G307D; hypothetical 21 atg acg tcc gtt aac
gtt aag ctc ctt tac cgt tat gtc tta acc aac 48 Met Thr Ser Val Asn
Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc aac
ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe Asn
Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 gcc
tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144 Ala
Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40
45 caa cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt ttc
192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe
50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg gtt tat
ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr
Leu Val 65 70 75 80 gac tac tcg tgt tac ctt ccg cca ccg cat ctc aga
gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Arg
Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc tac caa ata aga
aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg
Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac ggc acg tgt gat gat tcg
tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp Asp Ser
Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt tca ggt
cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg Ser Gly
Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt cag gtc
cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu Gln Val
Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 acg
gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc 528 Thr
Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170
175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc atg
576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met
180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt aac act
ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr
Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt aac ctt ggt ggc
atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly
Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att gat cta gca aag
gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys
Asp Leu Leu His Val His 225 230 235 240 aaa aat acg tat gct ctt gtg
gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala Leu Val
Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct ggt gat
aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala Gly Asp
Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt ggt
ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg Val Gly
Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 aga
cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga 912 Arg
Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295
300 gct gac gac aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac
960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn
305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc gat
gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr
Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg ttg
ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala Thr
Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt ttt
ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu
Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa
atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys Asp
Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 att
gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg 1200
Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385
390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta gag gca
tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu
Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act tca tct
agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser
Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa
gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala
Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct
tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp Gln Ile
Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg
gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa 1440 Trp
Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470
475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct gat tca ggt
aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser
Gly Lys 485 490 495 tca gag act cgt gtc caa aac ggt cgg tcc taa
1521 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 22 506 PRT
Artificial Sequence 5' 114 amino acids from A. thaliana FAE1 (SEQ
ID NO2) and 3' 392 amino acids from B. napus elongase KCS (SEQ ID
NO4) having mutations at positions 92 and 307; designated At114
K92R G307D; hypothetical 22 Met Thr Ser Val Asn Val Lys Leu Leu Tyr
Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro
Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile
Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn Leu
Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly Leu
Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80
Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Arg Val Ser Val Ser 85
90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser
Ser 100 105 110 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe
Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr
His Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr
Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile
Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn Val Asn Pro
Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn
Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205
Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210
215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val
His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn
Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met
Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu
Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu
Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys
Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly
Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330
335 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro
340 345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys
Lys Leu 355 360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp
Phe Lys Leu Ala 370 375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly
Arg Ala Val Ile Asp Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu
Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg
Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala
Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val
Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455
460 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu
465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp
Ser Gly Lys 485 490 495 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500
505 23 1521 DNA Artificial Sequence 5' 762 bp from A. thaliana FAE1
(SEQ ID NO1) and 3' 759 bp from B. napus elongase KCS (SEQ ID NO3);
designated At254 23 atg acg tcc gtt aac gtt aag ctc ctt tac cgt tac
gtc tta acc aac 48 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr
Val Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt ttg ttc ccg tta acg
gcg ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr
Ala Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg ctt acc ata aac gat
ctc cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp
Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 caa cac aac ctt ata aca
gta act tta ctc ttt gct ttc act gtt ttc 192 Gln His Asn Leu Ile Thr
Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc
tac atc gta acc cga ccc aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu
Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac
tcg tgt tac ctt cca cca ccg cat ctc aaa gtt agt gtc tct 288 Asp Tyr
Ser Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser Val Ser 85 90 95
aaa gtc atg gat att ttc tac caa ata aga aaa gct gat act tct tca 336
Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100
105 110 cgg aac gtg gca tgt gat gat ccg tcc tcg ctc gat ttc ctg agg
aag 384 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg
Lys 115 120 125 att caa gag cgt tca ggt cta ggt gat gag acg tac agt
cct gag gga 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser
Pro Glu Gly 130 135 140 ctc att cac gta cca ccg cgg aag act ttt gca
gcg tca cgt gaa gag 480 Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala
Ala Ser Arg Glu Glu 145 150 155 160 aca gag aag gtt atc atc ggt gcg
ctc gaa aat cta ttc gag aac acc 528 Thr Glu Lys Val Ile Ile Gly Ala
Leu Glu Asn Leu Phe Glu Asn Thr 165 170 175 aaa gtt aac cct aga gag
att ggt ata ctt gtg gtg aac tca agc atg 576 Lys Val Asn Pro Arg Glu
Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 ttt aat cca act
cct tcg cta tcc gct atg gtc gtt aat act ttc aag 624 Phe Asn Pro Thr
Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 ctc cga
agc aac atc aaa agc ttt aat cta gga gga atg ggt tgt agt 672 Leu Arg
Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220
gct ggt gtt att gcc att gat ttg gct aaa gac ttg ttg cat gtt cat 720
Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225
230 235 240 aaa aac act tat gct ctc gtg gtg agc aca gag aac atc act
tat aac 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr
Tyr Asn 245 250 255 att tac gct ggt gat aat agg tcc atg atg gtt tca
aat tgc ttg ttc 816 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser
Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gct att ttg ctc tcc
aac aag cct gga gat cgt 864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser
Asn Lys Pro Gly Asp Arg 275 280 285 aga cgg tcc aag tac gag cta gtt
cac acg gtt cga acg cat acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val
His Thr Val Arg Thr His Thr Gly 290 295 300 gct gac ggc aag tct ttt
cgt tgc gtg caa caa gga gac gat gag aac 960 Ala Asp Gly Lys Ser Phe
Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 ggc aaa atc
gga gtg agt ttg tcc aag gac ata acc gat gtt gct ggt 1008 Gly Lys
Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335
cga acg gtt aag aaa aac ata gca acg ttg ggt ccg ttg att ctt ccg
1056 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu
Pro 340 345 350 tta agc gag aaa ctt ctt ttt ttc gtt acc ttc atg ggc
aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met
Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa atc aaa cat tac tac gtc
ccg gat ttc aaa ctt gct 1152 Phe Lys Asp Lys Ile Lys His Tyr Tyr
Val Pro Asp Phe Lys Leu Ala 370 375 380 att gac cat ttt tgt ata cat
gcc gga ggc aga gcc gtg att gat gtg 1200 Ile Asp His Phe Cys Ile
His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 cta gag aag
aac cta gcc cta gca ccg atc gat gta gag gca tca aga 1248 Leu Glu
Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415
tca acg tta cat aga ttt gga aac act tca tct agc tca ata tgg tat
1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp
Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa gga agg atg aag aaa
ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys
Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct tta ggg tca ggc ttt
aag tgt aac agt gca gtt 1392 Val Trp Gln Ile Ala Leu Gly Ser Gly
Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta aac aat gtc
aaa gct tcg aca aat agt cct tgg gaa 1440 Trp Val Ala Leu Asn Asn
Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 cac tgc atc
gac aga tac ccg gtc aaa att gat tct gat tca ggt aag 1488 His Cys
Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495
tca gag act cgt gtc cca aac ggt cgg tcc taa 1521 Ser Glu Thr Arg
Val Pro Asn Gly Arg Ser 500 505 24 506 PRT Artificial Sequence 5'
254 amino acids from A. thaliana FAE1 (SEQ ID NO2) and 3' 252 amino
acids from B. napus elongase KCS (SEQ ID NO4); designated At254 24
Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5
10 15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly
Lys 20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu
Ser Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe
Ala Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg
Pro Asn Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro
Pro Pro His Leu Lys Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile
Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Val
Ala Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 Ile
Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135
140 Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu
145 150 155 160 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe
Glu Asn Thr 165 170 175 Lys Val Asn Pro Arg Glu Ile Gly Ile Leu Val
Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala
Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Ile Lys Ser
Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala
Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn
Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255
Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260
265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp
Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr
His Thr Gly 290 295 300 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln
Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser Leu Ser
Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys Lys Asn
Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys
Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 Phe Lys
Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380
Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385
390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala
Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg
Met Lys Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu Gly Ser
Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Asn Asn
Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His Cys Ile
Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 Ser
Glu Thr Arg Val Pro Asn Gly Arg Ser 500 505 25 1521 DNA Artificial
Sequence 5' 519 bp from A. thaliana FAE1 (SEQ ID NO1) and 3' 1002
bp from B. napus elongase KCS (SEQ ID NO3); designated At173 25 atg
acg tcc gtt aac gtt aag ctc ctt tac cgt tac gtc tta acc aac 48 Met
Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10
15 ttt ttc aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa
96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys
20 25 30 gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc
tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser
Tyr Leu 35 40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct
ttc act gtt ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala
Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc
aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro
Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt cca cca
ccg cat ctc aaa gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro
Pro His Leu Lys Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc
tac caa ata aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe
Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac gtg gca
tgt gat gat ccg tcc tcg ctc gat ttc ctg agg aag 384 Arg Asn Val Ala
Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 att caa
gag cgt tca ggt cta ggt gat gag acg tac agt cct gag gga 432 Ile Gln
Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140
ctc att cac gta cca ccg cgg aag act ttt gca gcg tca cgt gaa gag 480
Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145
150 155 160 aca gag aag gtt atc atc ggt gcg ctc gaa aat cta ttc aag
aac acc 528 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys
Asn Thr 165 170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg
aac tca agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val
Asn Ser Ser Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg
gtc gtt aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met
Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt
aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe
Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att
gat cta gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile
Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg
tat gct ctt gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr
Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att
tac gct ggt gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile
Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265
270 cgt gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt
864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg
275 280 285 aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat
acc gga 912
Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290
295 300 gct gac ggc aag tct ttt cgt tgc gtg caa caa gga gac gat gag
aac 960 Ala Asp Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu
Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc
gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile
Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg
ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala
Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt
ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu
Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat
aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys
Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380
att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg
1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta
gag gca tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act
tca tct agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa
gca aaa gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag
att gct tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa
1440 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp
Glu 465 470 475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct
gat tca ggt aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Ser Gly Lys 485 490 495 tca gag act cgt gtc cma aac ggt cgg
tcc taa 1521 Ser Glu Thr Arg Val Xaa Asn Gly Arg Ser 500 505 26 506
PRT Artificial Sequence 5' 173 amino acids from A. thaliana FAE1
(SEQ ID NO2) and 3' 333 amino acids from B. napus elongase KCS (SEQ
ID NO4); designated At173 26 Met Thr Ser Val Asn Val Lys Leu Leu
Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe
Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr
Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn
Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly
Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70
75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser Val
Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp
Thr Ser Ser 100 105 110 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser Leu
Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp
Glu Thr Tyr Ser Pro Glu Gly 130 135 140 Leu Ile His Val Pro Pro Arg
Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155 160 Thr Glu Lys Val
Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn Val
Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190
Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195
200 205 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys
Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu
His Val His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr
Glu Asn Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser
Met Met Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala
Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys
Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp
Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315
320 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly
325 330 335 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile
Leu Pro 340 345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met
Gly Lys Lys Leu 355 360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val
Pro Asp Phe Lys Leu Ala 370 375 380 Ile Asp His Phe Cys Ile His Ala
Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 Leu Glu Lys Asn Leu
Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu
His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu
Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440
445 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val
450 455 460 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro
Trp Glu 465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Ser Gly Lys 485 490 495 Ser Glu Thr Arg Val Xaa Asn Gly Arg
Ser 500 505 27 1521 DNA Artificial Sequence 5' 528 bp from B. napus
elongase KCS (SEQ ID NO3) and 3' 993 bp from A. thaliana FAE1 (SEQ
ID NO1); designated Bn176 27 atg acg tcc att aac gta aag ctc ctt
tac cat tac gtc ata acc aac 48 Met Thr Ser Ile Asn Val Lys Leu Leu
Tyr His Tyr Val Ile Thr Asn 1 5 10 15 ctt ttc aac ctt tgc ttc ttt
ccg tta acg gcg atc gtc gcc gga aaa 96 Leu Phe Asn Leu Cys Phe Phe
Pro Leu Thr Ala Ile Val Ala Gly Lys 20 25 30 gcc tat cgg ctt acc
ata gac gat ctt cac cac tta tac tat tcc tat 144 Ala Tyr Arg Leu Thr
Ile Asp Asp Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 ctc caa cac
aac ctc ata acc atc gct cca ctc ttt gcc ttc acc gtt 192 Leu Gln His
Asn Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 ttc
ggt tcg gtt ctc tac atc gca acc cgg ccc aaa ccg gtt tac ctc 240 Phe
Gly Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70
75 80 gtt gag tac tca tgc tac ctt cca cca acg cat tgt aga tca agt
atc 288 Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser
Ile 85 90 95 tcc aag gtc atg gat atc ttt tat caa gta aga aaa gct
gat cct tct 336 Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala
Asp Pro Ser 100 105 110 cgg aac ggc acg tgc gat gac tcg tcg tgg ctt
gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu
Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt tca ggt cta ggc gat
gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp
Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt cag gtc cct ccc cgg
aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu Gln Val Pro Pro Arg
Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 acg gag caa gtt
atc att ggt gcg cta gaa aat cta ttc aag aac acc 528 Thr Glu Gln Val
Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 aaa gtt
aac cct aga gag att ggt ata ctt gtg gtg aac tca agc atg 576 Lys Val
Asn Pro Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190
ttt aat cca act cct tcg cta tcc gct atg gtc gtt aat act ttc aag 624
Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195
200 205 ctc cga agc aac atc aaa agc ttt aat cta gga gga atg ggt tgt
agt 672 Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys
Ser 210 215 220 gct ggt gtt att gcc att gat ttg gct aaa gac ttg ttg
cat gtt cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu
His Val His 225 230 235 240 aaa aac act tat gct ctt gtg gtg agc act
gag aac atc aca caa ggc 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr
Glu Asn Ile Thr Gln Gly 245 250 255 att tat gct gga gaa aat aga tca
atg atg gtt agc aat tgc ttg ttt 816 Ile Tyr Ala Gly Glu Asn Arg Ser
Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gcg
att ttg ctc tct aac aag tcg gga gac cgg 864 Arg Val Gly Gly Ala Ala
Ile Leu Leu Ser Asn Lys Ser Gly Asp Arg 275 280 285 aga cgg tcc aag
tac aag cta gtt cac acg gtc cga acg cat act gga 912 Arg Arg Ser Lys
Tyr Lys Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 gct gat
gac aag tct ttt cga tgt gtg caa caa gaa gat gat gag agc 960 Ala Asp
Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 305 310 315
320 ggc aaa atc gga gtt tgt ctg tca aag gac ata acc aat gtt gcg ggg
1008 Gly Lys Ile Gly Val Cys Leu Ser Lys Asp Ile Thr Asn Val Ala
Gly 325 330 335 aca aca ctt acg aaa aat ata gca aca ttg ggt ccg ttg
att ctt cct 1056 Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu Gly Pro
Leu Ile Leu Pro 340 345 350 tta agc gaa aag ttt ctt ttt ttc gct acc
ttc gtc gcc aag aaa ctt 1104 Leu Ser Glu Lys Phe Leu Phe Phe Ala
Thr Phe Val Ala Lys Lys Leu 355 360 365 cta aag gat aaa atc aag cat
tac tat gtt ccg gat ttc aag ctt gct 1152 Leu Lys Asp Lys Ile Lys
His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 gtt gac cat ttc
tgt att cat gcc gga ggc aga gcc gtg atc gat gag 1200 Val Asp His
Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Glu 385 390 395 400
cta gag aag aac tta gga cta tcg ccg atc gat gtg gag gca tct aga
1248 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser
Arg 405 410 415 tca acg tta cat aga ttt ggg aat act tca tct agc tca
att tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 gaa tta gca tac ata gag gca aag gga aga
atg aag aaa ggg aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly
Arg Met Lys Lys Gly Asn Lys 435 440 445 gct tgg cag att gct tta gga
tca ggg ttt aag tgt aat agt gcg gtt 1392 Ala Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta
cgc aat gtc aag gca tcg gca aat agt cct tgg caa 1440 Trp Val Ala
Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp Gln 465 470 475 480
cat tgc atc gat aga tat ccg gtt aaa att gat tct gat ttg tca aag
1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser
Lys 485 490 495 tca aag act cat gtc caa aac ggt cgg tcc taa 1521
Ser Lys Thr His Val Gln Asn Gly Arg Ser 500 505 28 506 PRT
Artificial Sequence 5' 176 amino acids from B. napus elongase KCS
(SEQ ID NO4) and 3' 330 amino acids from A. thaliana FAE1 (SEQ ID
NO2); designated Bn176 28 Met Thr Ser Ile Asn Val Lys Leu Leu Tyr
His Tyr Val Ile Thr Asn 1 5 10 15 Leu Phe Asn Leu Cys Phe Phe Pro
Leu Thr Ala Ile Val Ala Gly Lys 20 25 30 Ala Tyr Arg Leu Thr Ile
Asp Asp Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 Leu Gln His Asn
Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 Phe Gly
Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70 75 80
Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile 85
90 95 Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro
Ser 100 105 110 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe
Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr
His Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr
Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile
Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Lys Val Asn Pro
Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn
Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205
Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210
215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val
His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn
Ile Thr Gln Gly 245 250 255 Ile Tyr Ala Gly Glu Asn Arg Ser Met Met
Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu
Leu Ser Asn Lys Ser Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Lys
Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys
Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 305 310 315 320 Gly
Lys Ile Gly Val Cys Leu Ser Lys Asp Ile Thr Asn Val Ala Gly 325 330
335 Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro
340 345 350 Leu Ser Glu Lys Phe Leu Phe Phe Ala Thr Phe Val Ala Lys
Lys Leu 355 360 365 Leu Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp
Phe Lys Leu Ala 370 375 380 Val Asp His Phe Cys Ile His Ala Gly Gly
Arg Ala Val Ile Asp Glu 385 390 395 400 Leu Glu Lys Asn Leu Gly Leu
Ser Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg
Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala
Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Ala
Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455
460 Trp Val Ala Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp Gln
465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp
Leu Ser Lys 485 490 495 Ser Lys Thr His Val Gln Asn Gly Arg Ser 500
505 29 1521 DNA Artificial Sequence 5' 1197 bp from A. thaliana
FAE1 (SEQ ID NO1) and 3' 324 bp from B. napus elongase KCS (SEQ ID
NO3); designated At399 29 atg acg tcc gtt aac gtt aag ctc ctt tac
cgt tac gtc tta acc aac 48 Met Thr Ser Val Asn Val Lys Leu Leu Tyr
Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt ttg ttc ccg
tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys Leu Phe Pro
Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg ctt acc ata
aac gat ctc cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg Leu Thr Ile
Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 caa cac aac ctt
ata aca gta act tta ctc ttt gct ttc act gtt ttc 192 Gln His Asn Leu
Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 ggt ttg
gtt ctc tac atc gta acc cga ccc aat ccg gtt tat ctc gtt 240 Gly Leu
Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80
gac tac tcg tgt tac ctt cca cca ccg cat ctc aaa gtt agt gtc tct 288
Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser Val Ser 85
90 95 aaa gtc atg gat att ttc tac caa ata aga aaa gct
gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala
Asp Thr Ser Ser 100 105 110 cgg aac gtg gca tgt gat gat ccg tcc tcg
ctc gat ttc ctg agg aag 384 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser
Leu Asp Phe Leu Arg Lys 115 120 125 att caa gag cgt tca ggt cta ggt
gat gag acg tac agt cct gag gga 432 Ile Gln Glu Arg Ser Gly Leu Gly
Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140 ctc att cac gta cca ccg
cgg aag act ttt gca gcg tca cgt gaa gag 480 Leu Ile His Val Pro Pro
Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155 160 aca gag aag
gtt atc atc ggt gcg ctc gaa aat cta ttc gag aac acc 528 Thr Glu Lys
Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Glu Asn Thr 165 170 175 aaa
gtt aac cct aga gag att ggt ata ctt gtg gtg aac tca agc atg 576 Lys
Val Asn Pro Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185
190 ttt aat cca act cct tcg cta tcc gct atg gtc gtt aat act ttc aag
624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys
195 200 205 ctc cga agc aac atc aaa agc ttt aat cta gga gga atg ggt
tgt agt 672 Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly
Cys Ser 210 215 220 gct ggt gtt att gcc att gat ttg gct aaa gac ttg
ttg cat gtt cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu
Leu His Val His 225 230 235 240 aaa aac act tat gct ctt gtg gtg agc
act gag aac atc aca caa ggc 768 Lys Asn Thr Tyr Ala Leu Val Val Ser
Thr Glu Asn Ile Thr Gln Gly 245 250 255 att tat gct gga gaa aat aga
tca atg atg gtt agc aat tgc ttg ttt 816 Ile Tyr Ala Gly Glu Asn Arg
Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc
gcg att ttg ctc tct aac aag tcg gga gac cgg 864 Arg Val Gly Gly Ala
Ala Ile Leu Leu Ser Asn Lys Ser Gly Asp Arg 275 280 285 aga cgg tcc
aag tac aag cta gtt cac acg gtc cga acg cat act gga 912 Arg Arg Ser
Lys Tyr Lys Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 gct
gat gac aag tct ttt cga tgt gtg caa caa gaa gac gat gag agc 960 Ala
Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 305 310
315 320 ggc aaa atc gga gtt tgt ctg tca aag gac ata acc aat gtt gcg
ggg 1008 Gly Lys Ile Gly Val Cys Leu Ser Lys Asp Ile Thr Asn Val
Ala Gly 325 330 335 aca aca ctt acg aaa aat ata gca aca ttg ggt ccg
ttg att ctt cct 1056 Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu Gly
Pro Leu Ile Leu Pro 340 345 350 tta agc gaa aag ttt ctt ttt ttc gct
acc ttc gtc gcc aag aaa ctt 1104 Leu Ser Glu Lys Phe Leu Phe Phe
Ala Thr Phe Val Ala Lys Lys Leu 355 360 365 cta aag gat aaa atc aag
cat tac tat gtt ccg gat ttc aag ctt gct 1152 Leu Lys Asp Lys Ile
Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 gtt gac cat
ttc tgt att cat gcc gga ggc aga gcc gtg atc gat gtg 1200 Val Asp
His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395
400 cta gag aag aac cta gcc cta gca ccg atc gat gta gag gca tca aga
1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser
Arg 405 410 415 tca acg tta cat aga ttt gga aac act tca tct agc tca
ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa gga agg
atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly
Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct tta ggg
tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta
aac aat gtc aaa gct tcg aca aat agt cct tgg gaa 1440 Trp Val Ala
Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480
cac tgc atc gac aga tac ccg gtc aaa att gat tct gat tca ggt aag
1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly
Lys 485 490 495 tca gag act cgt gtc cma aac ggt cgg tcc taa 1521
Ser Glu Thr Arg Val Xaa Asn Gly Arg Ser 500 505 30 506 PRT
Artificial Sequence 5' 399 amino acids from A. thaliana FAE1 (SEQ
ID NO2) and 3' 107 amino acids from B. napus elongase KCS (SEQ ID
NO4); designated At399 30 Met Thr Ser Val Asn Val Lys Leu Leu Tyr
Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro
Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile
Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn Leu
Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly Leu
Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80
Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser Val Ser 85
90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser
Ser 100 105 110 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser Leu Asp Phe
Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr
Tyr Ser Pro Glu Gly 130 135 140 Leu Ile His Val Pro Pro Arg Lys Thr
Phe Ala Ala Ser Arg Glu Glu 145 150 155 160 Thr Glu Lys Val Ile Ile
Gly Ala Leu Glu Asn Leu Phe Glu Asn Thr 165 170 175 Lys Val Asn Pro
Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn
Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205
Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210
215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val
His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn
Ile Thr Gln Gly 245 250 255 Ile Tyr Ala Gly Glu Asn Arg Ser Met Met
Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu
Leu Ser Asn Lys Ser Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Lys
Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys
Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu Ser 305 310 315 320 Gly
Lys Ile Gly Val Cys Leu Ser Lys Asp Ile Thr Asn Val Ala Gly 325 330
335 Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro
340 345 350 Leu Ser Glu Lys Phe Leu Phe Phe Ala Thr Phe Val Ala Lys
Lys Leu 355 360 365 Leu Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp
Phe Lys Leu Ala 370 375 380 Val Asp His Phe Cys Ile His Ala Gly Gly
Arg Ala Val Ile Asp Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu
Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg
Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala
Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val
Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455
460 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu
465 470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp
Ser Gly Lys 485 490 495 Ser Glu Thr Arg Val Xaa Asn Gly Arg Ser 500
505 31 1521 DNA Artificial Sequence 5' 1197 bp from B. napus
elongase KCS (SEQ ID NO3) and 3' 324 bp from A. thaliana FAE1 (SEQ
ID NO1); designated Bn399 31 atg acg tcc att aac gtt aag ctc ctt
tac cat tac gtc ata acc aac 48 Met Thr Ser Ile Asn Val Lys Leu Leu
Tyr His Tyr Val Ile Thr Asn 1 5 10 15 ctt ttc aac ctt tgc ttc ttt
ccg tta acg gcg atc gtc gcc gga aaa 96 Leu Phe Asn Leu Cys Phe Phe
Pro Leu Thr Ala Ile Val Ala Gly Lys 20 25 30 gcc tat cgg ctt acc
ata gac gat ctt cac cac tta tac tat tcc tat 144 Ala Tyr Arg Leu Thr
Ile Asp Asp Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 ctc caa cac
aac ctc ata acc atc gct cca ctc ttt gcc ttc acc gtt 192 Leu Gln His
Asn Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 ttc
ggt tcg gtt ctc tac atc gca acc cgg ccc aaa ccg gtt tac ctc 240 Phe
Gly Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70
75 80 gtt gag tac tca tgc tac ctt cca cca acg cat tgt aga tca agt
atc 288 Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser
Ile 85 90 95 tcc aag gtc atg gat atc ttt tat caa gta aga aaa gct
gat cct tct 336 Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala
Asp Pro Ser 100 105 110 cgg aac ggc acg tgc gat gac tcg tcg tgg ctt
gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu
Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt tca ggt cta ggc gat
gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp
Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt cag gtc cct ccc cgg
aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu Gln Val Pro Pro Arg
Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 acg gag caa gtt
atc att ggt gcg cta gaa aat cta ttc aag aac acc 528 Thr Glu Gln Val
Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 aac gtt
aac cct aaa gat ata ggt ata ctt gtg gtg aac tca agc atg 576 Asn Val
Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190
ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt aac act ttc aag 624
Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195
200 205 ctc cga agc aac gta aga agc ttt aac ctt ggt ggc atg ggt tgt
agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys
Ser 210 215 220 gcc ggc gtt ata gcc att gat cta gca aag gac ttg ttg
cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu
His Val His 225 230 235 240 aaa aat acg tat gct ctt gtg gtg agc aca
gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr
Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct ggt gat aat agg tcc
atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala Gly Asp Asn Arg Ser
Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gct
att ttg ctc tcc aac aag cct gga gat cgt 864 Arg Val Gly Gly Ala Ala
Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 aga cgg tcc aag
tac gag cta gtt cac acg gtt cga acg cat acc gga 912 Arg Arg Ser Lys
Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 gct gac
ggc aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac 960 Ala Asp
Gly Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315
320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc gat gtt gct ggt
1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala
Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg ttg ggt ccg ttg
att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro
Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt ttt ttc gtt acc
ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu Phe Phe Val
Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa atc aaa cat
tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys Asp Lys Ile Lys
His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 att gac cat ttt
tgt ata cat gcc gga ggc aga gcc gtg atc gat gag 1200 Ile Asp His
Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Glu 385 390 395 400
cta gag aag aac tta gga cta tcg ccg atc gat gtg gag gca tct aga
1248 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser
Arg 405 410 415 tca acg tta cat aga ttt ggg aat act tca tct agc tca
att tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 gaa tta gca tac ata gag gca aag gga aga
atg aag aaa ggg aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly
Arg Met Lys Lys Gly Asn Lys 435 440 445 gct tgg cag att gct tta gga
tca ggg ttt aag tgt aat agt gcg gtt 1392 Ala Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta
cgc aat gtc aag gca tcg gca aat agt cct tgg caa 1440 Trp Val Ala
Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp Gln 465 470 475 480
cat tgc atc gat aga tat ccg gtt aaa att gat tct gat ttg tca aag
1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser
Lys 485 490 495 tca aag act cat gtc caa aac ggt cgg tcc taa 1521
Ser Lys Thr His Val Gln Asn Gly Arg Ser 500 505 32 506 PRT
Artificial Sequence 5' 399 amino acids from B. napus elongase KCS
(SEQ ID NO3) and 3' 107 amino acids from A. thaliana FAE1 (SEQ ID
NO1); designated Bn399 32 Met Thr Ser Ile Asn Val Lys Leu Leu Tyr
His Tyr Val Ile Thr Asn 1 5 10 15 Leu Phe Asn Leu Cys Phe Phe Pro
Leu Thr Ala Ile Val Ala Gly Lys 20 25 30 Ala Tyr Arg Leu Thr Ile
Asp Asp Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 Leu Gln His Asn
Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 Phe Gly
Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70 75 80
Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile 85
90 95 Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro
Ser 100 105 110 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe
Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr
His Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr
Phe Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile
Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn Val Asn Pro
Lys Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn
Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205
Leu Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210
215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val
His 225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn
Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met
Val Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu
Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu
Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Gly Lys
Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly
Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330
335 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro
340 345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys
Lys Leu 355 360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp
Phe Lys Leu Ala 370 375 380 Ile Asp
His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Glu 385 390 395
400 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser Arg
405 410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile
Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys
Lys Gly Asn Lys 435 440 445 Ala Trp Gln Ile Ala Leu Gly Ser Gly Phe
Lys Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Arg Asn Val Lys
Ala Ser Ala Asn Ser Pro Trp Gln 465 470 475 480 His Cys Ile Asp Arg
Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser Lys 485 490 495 Ser Lys Thr
His Val Gln Asn Gly Arg Ser 500 505 33 1524 DNA Artificial Sequence
1524 bp from B. napus elongase KCS (SEQ ID NO3) having a mutation
at position 920; designated Bn G307D; hypothetical 33 atg acg tcc
att aac gta aag ctc ctt tac cat tac gtc ata acc aac 48 Met Thr Ser
Ile Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 1 5 10 15 ctt
ttc aac ctt tgc ttc ttt ccg tta acg gcg atc gtc gcc gga aaa 96 Leu
Phe Asn Leu Cys Phe Phe Pro Leu Thr Ala Ile Val Ala Gly Lys 20 25
30 gcc tat cgg ctt acc ata gac gat ctt cac cac tta tac tat tcc tat
144 Ala Tyr Arg Leu Thr Ile Asp Asp Leu His His Leu Tyr Tyr Ser Tyr
35 40 45 ctc caa cac aac ctc ata acc atc gct cca ctc ttt gcc ttc
acc gtt 192 Leu Gln His Asn Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe
Thr Val 50 55 60 ttc ggt tcg gtt ctc tac atc gca acc cgg ccc aaa
ccg gtt tac ctc 240 Phe Gly Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys
Pro Val Tyr Leu 65 70 75 80 gtt gag tac tca tgc tac ctt cca cca acg
cat tgt aga tca agt atc 288 Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr
His Cys Arg Ser Ser Ile 85 90 95 tcc aag gtc atg gat atc ttt tat
caa gta aga aaa gct gat cct tct 336 Ser Lys Val Met Asp Ile Phe Tyr
Gln Val Arg Lys Ala Asp Pro Ser 100 105 110 cgg aac ggc acg tgc gat
gac tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp
Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt
tca ggt cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg
Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt
cag gtc cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu
Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155
160 acg gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc
528 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr
165 170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca
agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser
Ser Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt
aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val
Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt aac ctt
ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu
Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att gat cta
gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu
Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg tat gct
ctt gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala
Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct
ggt gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala
Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt
gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg
Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280
285 aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga
912 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly
290 295 300 gct gac gac aag tct ttt cgt tgc gtg caa caa gga gac gat
gag aac 960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp
Glu Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata
acc gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp
Ile Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca
acg ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile
Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt
ctt ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys
Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa
gat aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe
Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375
380 att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg
1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Val 385 390 395 400 cta gag aag aac cta gcc cta gca ccg atc gat gta
gag gca tca aga 1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt gga aac act
tca tct agc tca ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa
gca aaa gga agg atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag
att gct tta ggg tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta aac aat gtc aaa gct tcg aca aat agt cct tgg gaa
1440 Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp
Glu 465 470 475 480 cac tgc atc gac aga tac ccg gtc aaa att gat tct
gat tca ggt aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Ser Gly Lys 485 490 495 tca gag act cgt gtc caa aac ggt cgg
tcc taataa 1524 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 34
506 PRT Artificial Sequence 506 amino acids from B. napus elongase
KCS (SEQ ID NO4) having a mutation at residue 307; designated Bn
G307D; hypothetical 34 Met Thr Ser Ile Asn Val Lys Leu Leu Tyr His
Tyr Val Ile Thr Asn 1 5 10 15 Leu Phe Asn Leu Cys Phe Phe Pro Leu
Thr Ala Ile Val Ala Gly Lys 20 25 30 Ala Tyr Arg Leu Thr Ile Asp
Asp Leu His His Leu Tyr Tyr Ser Tyr 35 40 45 Leu Gln His Asn Leu
Ile Thr Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 Phe Gly Ser
Val Leu Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70 75 80 Val
Glu Tyr Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile 85 90
95 Ser Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro Ser
100 105 110 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu
Arg Lys 115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His
Gly Pro Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe
Ala Ala Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile Gly
Ala Leu Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn Val Asn Pro Lys
Asp Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn Pro
Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 Leu
Arg Ser Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215
220 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His
225 230 235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile
Thr Tyr Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val
Ser Asn Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu
Ser Asn Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu
Val His Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys Ser
Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys
Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335
Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340
345 350 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys
Leu 355 360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe
Lys Leu Ala 370 375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg
Ala Val Ile Asp Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala
Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg Phe
Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr
Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
Trp Val Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465
470 475 480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser
Gly Lys 485 490 495 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505
35 1709 DNA Artificial Sequence 1709 bp from A. thaliana FAE1 (SEQ
ID NO1) having a mutation at position 275; designated At K92R;
hypothetical 35 atg acg tcc gtt aac gtt aag ctc ctt tac cgt tac gtc
tta acc aac 48 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val
Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt ttg ttc ccg tta acg gcg
ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala
Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg ctt acc ata aac gat ctc
cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp Leu
His Asn Phe Leu Ser Tyr Leu 35 40 45 caa cac aac ctt ata aca gta
act tta ctc ttt gct ttc act gtt ttc 192 Gln His Asn Leu Ile Thr Val
Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc tac
atc gta acc cga ccc aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu Tyr
Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac tcg
tgt tac ctt cca cca ccg cat ctc aga gtt agt gtc tct 288 Asp Tyr Ser
Cys Tyr Leu Pro Pro Pro His Leu Arg Val Ser Val Ser 85 90 95 aaa
gtc atg gat att ttc tac caa ata aga aaa gct gat act tct tca 336 Lys
Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105
110 cgg aac gtg gca tgt gat gat ccg tcc tcg ctc gat ttc ctg agg aag
384 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys
115 120 125 att caa gag cgt tca ggt cta ggt gat gag acg tac agt cct
gag gga 432 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro
Glu Gly 130 135 140 ctc att cac gta cca ccg cgg aag act ttt gca gcg
tca cgt gaa gag 480 Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala
Ser Arg Glu Glu 145 150 155 160 aca gag aag gtt atc atc ggt gcg ctc
gaa aat cta ttc gag aac acc 528 Thr Glu Lys Val Ile Ile Gly Ala Leu
Glu Asn Leu Phe Glu Asn Thr 165 170 175 aaa gtt aac cct aga gag att
ggt ata ctt gtg gtg aac tca agc atg 576 Lys Val Asn Pro Arg Glu Ile
Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 ttt aat cca act cct
tcg cta tcc gct atg gtc gtt aat act ttc aag 624 Phe Asn Pro Thr Pro
Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc
aac atc aaa agc ttt aat cta gga gga atg ggt tgt agt 672 Leu Arg Ser
Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gct
ggt gtt att gcc att gat ttg gct aaa gac ttg ttg cat gtt cat 720 Ala
Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230
235 240 aaa aac act tat gct ctt gtg gtg agc act gag aac atc aca caa
ggc 768 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Gln
Gly 245 250 255 att tat gct gga gaa aat aga tca atg atg gtt agc aat
tgc ttg ttt 816 Ile Tyr Ala Gly Glu Asn Arg Ser Met Met Val Ser Asn
Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc gcg att ttg ctc tct aac
aag tcg gga gac cgg 864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn
Lys Ser Gly Asp Arg 275 280 285 aga cgg tcc aag tac aag cta gtt cac
acg gtc cga acg cat act gga 912 Arg Arg Ser Lys Tyr Lys Leu Val His
Thr Val Arg Thr His Thr Gly 290 295 300 gct gat gac aag tct ttt cga
tgt gtg caa caa gaa gac gat gag agc 960 Ala Asp Asp Lys Ser Phe Arg
Cys Val Gln Gln Glu Asp Asp Glu Ser 305 310 315 320 ggc aaa atc gga
gtt tgt ctg tca aag gac ata acc aat gtt gcg ggg 1008 Gly Lys Ile
Gly Val Cys Leu Ser Lys Asp Ile Thr Asn Val Ala Gly 325 330 335 aca
aca ctt acg aaa aat ata gca aca ttg ggt ccg ttg att ctt cct 1056
Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340
345 350 tta agc gaa aag ttt ctt ttt ttc gct acc ttc gtc gcc aag aaa
ctt 1104 Leu Ser Glu Lys Phe Leu Phe Phe Ala Thr Phe Val Ala Lys
Lys Leu 355 360 365 cta aag gat aaa atc aag cat tac tat gtt ccg gat
ttc aag ctt gct 1152 Leu Lys Asp Lys Ile Lys His Tyr Tyr Val Pro
Asp Phe Lys Leu Ala 370 375 380 gtt gac cat ttc tgt att cat gcc gga
ggc aga gcc gtg atc gat gag 1200 Val Asp His Phe Cys Ile His Ala
Gly Gly Arg Ala Val Ile Asp Glu 385 390 395 400 cta gag aag aac tta
gga cta tcg ccg atc gat gtg gag gca tct aga 1248 Leu Glu Lys Asn
Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 tca acg
tta cat aga ttt ggg aat act tca tct agc tca att tgg tat 1296 Ser
Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425
430 gaa tta gca tac ata gag gca aag gga aga atg aag aaa ggg aat aaa
1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn
Lys 435 440 445 gct tgg cag att gct tta gga tca ggg ttt aag tgt aat
agt gcg gtt 1392 Ala Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys
Asn Ser Ala Val 450 455 460 tgg gtg gct cta cgc aat gtc aag gca tcg
gca aat agt cct tgg caa 1440 Trp Val Ala Leu Arg Asn Val Lys Ala
Ser Ala Asn Ser Pro Trp Gln 465 470 475 480 cat tgc atc gat aga tat
ccg gtt aaa att gat tct gat ttg tca aag 1488 His Cys Ile Asp Arg
Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser Lys 485 490 495 tca aag act
cat gtc caa aac ggt cgg tcc taatttgatg tatctgagtg 1538 Ser Lys Thr
His Val Gln Asn Gly Arg Ser 500 505 ccaacgttta ctttgtcttt
cctttctttt attggttatg aattagatgt ttactaatgt 1598 tcctctcttt
ttcgttataa ataaagaagt tcaattcttc ctatagtttc aaacgcgatt 1658
ttaagcgttt ctatttaggt ttacatgaat ttcttttaca aaccatcttt t 1709 36
506 PRT Artificial Sequence 506 amino acids from A. thaliana FAE1
(SEQ ID NO2) having a mutation at residue 92; designated At K92R;
hypothetical 36 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val
Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala
Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu
His Asn Phe Leu Ser Tyr Leu 35
40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val
Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val
Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu
Arg Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr Gln Ile
Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Val Ala Cys Asp Asp
Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu Arg Ser
Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140 Leu Ile His
Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155 160
Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Glu Asn Thr 165
170 175 Lys Val Asn Pro Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser
Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn
Thr Phe Lys 195 200 205 Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly
Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp Leu Ala
Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn Thr Tyr Ala Leu
Val Val Ser Thr Glu Asn Ile Thr Gln Gly 245 250 255 Ile Tyr Ala Gly
Glu Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 Arg Val
Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Ser Gly Asp Arg 275 280 285
Arg Arg Ser Lys Tyr Lys Leu Val His Thr Val Arg Thr His Thr Gly 290
295 300 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Glu Asp Asp Glu
Ser 305 310 315 320 Gly Lys Ile Gly Val Cys Leu Ser Lys Asp Ile Thr
Asn Val Ala Gly 325 330 335 Thr Thr Leu Thr Lys Asn Ile Ala Thr Leu
Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys Phe Leu Phe Phe
Ala Thr Phe Val Ala Lys Lys Leu 355 360 365 Leu Lys Asp Lys Ile Lys
His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 Val Asp His Phe
Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Glu 385 390 395 400 Leu
Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp Val Glu Ala Ser Arg 405 410
415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr
420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly
Asn Lys 435 440 445 Ala Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys
Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Arg Asn Val Lys Ala Ser
Ala Asn Ser Pro Trp Gln 465 470 475 480 His Cys Ile Asp Arg Tyr Pro
Val Lys Ile Asp Ser Asp Leu Ser Lys 485 490 495 Ser Lys Thr His Val
Gln Asn Gly Arg Ser 500 505 37 1521 DNA Artificial Sequence 5' 762
bp from A. thaliana FAE1 (SEQ ID NO1) and 3' 759 bp from B. napus
elongase KCS (SEQ ID NO3) and having a mutation at position 920;
designated At254 G307D; hypothetical 37 atg acg tcc gtt aac gtt aag
ctc ctt tac cgt tac gtc tta acc aac 48 Met Thr Ser Val Asn Val Lys
Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 ttt ttc aac ctc tgt
ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa 96 Phe Phe Asn Leu Cys
Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25 30 gcc tct cgg
ctt acc ata aac gat ctc cac aac ttc ctt tcc tat ctc 144 Ala Ser Arg
Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu 35 40 45 caa
cac aac ctt ata aca gta act tta ctc ttt gct ttc act gtt ttc 192 Gln
His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55
60 ggt ttg gtt ctc tac atc gta acc cga ccc aat ccg gtt tat ctc gtt
240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val
65 70 75 80 gac tac tcg tgt tac ctt cca cca ccg cat ctc aaa gtt agt
gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser
Val Ser 85 90 95 aaa gtc atg gat att ttc tac caa ata aga aaa gct
gat act tct tca 336 Lys Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala
Asp Thr Ser Ser 100 105 110 cgg aac gtg gca tgt gat gat ccg tcc tcg
ctc gat ttc ctg agg aag 384 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser
Leu Asp Phe Leu Arg Lys 115 120 125 att caa gag cgt tca ggt cta ggt
gat gag acg tac agt cct gag gga 432 Ile Gln Glu Arg Ser Gly Leu Gly
Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140 ctc att cac gta cca ccg
cgg aag act ttt gca gcg tca cgt gaa gag 480 Leu Ile His Val Pro Pro
Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155 160 aca gag aag
gtt atc atc ggt gcg ctc gaa aat cta ttc gag aac acc 528 Thr Glu Lys
Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Glu Asn Thr 165 170 175 aaa
gtt aac cct aga gag att ggt ata ctt gtg gtg aac tca agc atg 576 Lys
Val Asn Pro Arg Glu Ile Gly Ile Leu Val Val Asn Ser Ser Met 180 185
190 ttt aat cca act cct tcg cta tcc gct atg gtc gtt aat act ttc aag
624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys
195 200 205 ctc cga agc aac atc aaa agc ttt aat cta gga gga atg ggt
tgt agt 672 Leu Arg Ser Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly
Cys Ser 210 215 220 gct ggt gtt att gcc att gat ttg gct aaa gac ttg
ttg cat gtt cat 720 Ala Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu
Leu His Val His 225 230 235 240 aaa aac act tat gct ctc gtg gtg agc
aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala Leu Val Val Ser
Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct ggt gat aat agg
tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala Gly Asp Asn Arg
Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt gtt ggt ggg gcc
gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg Val Gly Gly Ala
Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280 285 aga cgg tcc
aag tac gag cta gtt cac acg gtt cga acg cat acc gga 912 Arg Arg Ser
Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly 290 295 300 gct
gac gac aag tct ttt cgt tgc gtg caa caa gga gac gat gag aac 960 Ala
Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310
315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata acc gat gtt gct
ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile Thr Asp Val
Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca acg ttg ggt ccg
ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly
Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt ctt ttt ttc gtt
acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys Leu Leu Phe Phe
Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa gat aaa atc aaa
cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe Lys Asp Lys Ile
Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 att gac cat
ttt tgt ata cat gcc gga ggc aga gcc gtg att gat gtg 1200 Ile Asp
His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395
400 cta gag aag aac cta gcc cta gca ccg atc gat gta gag gca tca aga
1248 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser
Arg 405 410 415 tca acg tta cat aga ttt gga aac act tca tct agc tca
ata tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser
Ser Ile Trp Tyr 420 425 430 gag ttg gca tac ata gaa gca aaa gga agg
atg aag aaa ggt aat aaa 1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly
Arg Met Lys Lys Gly Asn Lys 435 440 445 gtt tgg cag att gct tta ggg
tca ggc ttt aag tgt aac agt gca gtt 1392 Val Trp Gln Ile Ala Leu
Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 tgg gtg gct cta
aac aat gtc aaa gct tcg aca aat agt cct tgg gaa 1440 Trp Val Ala
Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475 480
cac tgc atc gac aga tac ccg gtc aaa att gat tct gat tca ggt aag
1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly
Lys 485 490 495 tca gag act cgt gtc caa aac ggt cgg tcc taa 1521
Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 38 506 PRT
Artificial Sequence 5' 254 amino acids from A. thaliana FAE1 (SEQ
ID NO2) and 3' 252 amino acids from B. napus elongase KCS (SEQ ID
NO4) having a mutation at residue 307; designated At254 G307D;
hypothetical 38 Met Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val
Leu Thr Asn 1 5 10 15 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala
Phe Leu Ala Gly Lys 20 25 30 Ala Ser Arg Leu Thr Ile Asn Asp Leu
His Asn Phe Leu Ser Tyr Leu 35 40 45 Gln His Asn Leu Ile Thr Val
Thr Leu Leu Phe Ala Phe Thr Val Phe 50 55 60 Gly Leu Val Leu Tyr
Ile Val Thr Arg Pro Asn Pro Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser
Cys Tyr Leu Pro Pro Pro His Leu Lys Val Ser Val Ser 85 90 95 Lys
Val Met Asp Ile Phe Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105
110 Arg Asn Val Ala Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys
115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro
Glu Gly 130 135 140 Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala
Ser Arg Glu Glu 145 150 155 160 Thr Glu Lys Val Ile Ile Gly Ala Leu
Glu Asn Leu Phe Glu Asn Thr 165 170 175 Lys Val Asn Pro Arg Glu Ile
Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro
Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser
Asn Ile Lys Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala
Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230
235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr
Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn
Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn
Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His
Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys Ser Phe Arg
Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly
Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr
Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350
Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355
360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu
Ala 370 375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val
Ile Asp Val 385 390 395 400 Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile
Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu
Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Val Trp Gln Ile
Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val
Ala Leu Asn Asn Val Lys Ala Ser Thr Asn Ser Pro Trp Glu 465 470 475
480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys
485 490 495 Ser Glu Thr Arg Val Gln Asn Gly Arg Ser 500 505 39 1521
DNA Artificial Sequence 5' 519 bp from A. thaliana FAE1 (SEQ ID
NO1) and 3' 1002 bp from B. napus elongase KCS (SEQ ID NO3) and
having a mutation at position 920; designated At173 G307D 39 atg
acg tcc gtt aac gtt aag ctc ctt tac cgt tac gtc tta acc aac 48 Met
Thr Ser Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10
15 ttt ttc aac ctc tgt ttg ttc ccg tta acg gcg ttc ctc gcc gga aaa
96 Phe Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys
20 25 30 gcc tct cgg ctt acc ata aac gat ctc cac aac ttc ctt tcc
tat ctc 144 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser
Tyr Leu 35 40 45 caa cac aac ctt ata aca gta act tta ctc ttt gct
ttc act gtt ttc 192 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala
Phe Thr Val Phe 50 55 60 ggt ttg gtt ctc tac atc gta acc cga ccc
aat ccg gtt tat ctc gtt 240 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro
Asn Pro Val Tyr Leu Val 65 70 75 80 gac tac tcg tgt tac ctt cca cca
ccg cat ctc aaa gtt agt gtc tct 288 Asp Tyr Ser Cys Tyr Leu Pro Pro
Pro His Leu Lys Val Ser Val Ser 85 90 95 aaa gtc atg gat att ttc
tac caa ata aga aaa gct gat act tct tca 336 Lys Val Met Asp Ile Phe
Tyr Gln Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 cgg aac gtg gca
tgt gat gat ccg tcc tcg ctc gat ttc ctg agg aag 384 Arg Asn Val Ala
Cys Asp Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 att caa
gag cgt tca ggt cta ggt gat gag acg tac agt cct gag gga 432 Ile Gln
Glu Arg Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140
ctc att cac gta cca ccg cgg aag act ttt gca gcg tca cgt gaa gag 480
Leu Ile His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145
150 155 160 aca gag aag gtt atc atc ggt gcg ctc gaa aat cta ttc aag
aac acc 528 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys
Asn Thr 165 170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg
aac tca agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val
Asn Ser Ser Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg
gtc gtt aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met
Val Val Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt
aac ctt ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe
Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att
gat cta gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile
Asp Leu Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg
tat gct ctt gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr
Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att
tac gct ggt gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile
Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265
270 cgt gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt
864 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg
275 280 285 aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat
acc gga 912 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His
Thr Gly 290 295 300 gct gac gac aag tct ttt cgt tgc gtg caa caa gga
gac gat gag aac 960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly
Asp Asp Glu Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag
gac ata acc gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser
Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 cga
acg gtt aag aaa aac ata gca acg ttg ggt ccg ttg att ctt ccg 1056
Arg Thr Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340
345 350 tta agc gag aaa ctt ctt ttt ttc gtt acc ttc atg ggc aag aaa
ctt 1104 Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys
Lys Leu 355 360 365 ttc aaa gat aaa atc aaa cat tac tac gtc ccg gat
ttc aaa ctt gct 1152 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro
Asp Phe Lys Leu Ala 370 375 380 att gac cat ttt tgt ata cat gcc gga
ggc aga gcc gtg att gat gtg 1200 Ile Asp His Phe Cys Ile His Ala
Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400 cta gag aag aac cta
gcc cta gca ccg atc gat gta gag gca tca aga 1248 Leu Glu Lys Asn
Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405 410 415 tca acg
tta cat aga ttt gga aac act tca tct agc tca ata tgg tat 1296 Ser
Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp Tyr 420 425
430 gag ttg gca tac ata gaa gca aaa gga agg atg aag aaa ggt aat aaa
1344 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys Gly Asn
Lys 435 440 445 gtt tgg cag att gct tta ggg tca ggc ttt aag tgt aac
agt gca gtt 1392 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys
Asn Ser Ala Val 450 455 460 tgg gtg gct cta aac aat gtc aaa gct tcg
aca aat agt cct tgg gaa 1440 Trp Val Ala Leu Asn Asn Val Lys Ala
Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 cac tgc atc gac aga tac
ccg gtc aaa att gat tct gat tca ggt aag 1488 His Cys Ile Asp Arg
Tyr Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 tca gag act
cgt gtc caa aac ggt cgg tcc taa 1521 Ser Glu Thr Arg Val Gln Asn
Gly Arg Ser 500 505 40 506 PRT Artificial Sequence 5' 173 amino
acids from A. thaliana FAE1 (SEQ ID NO2) and 3' 333 amino acids
from B. napus elongase KCS (SEQ ID NO4) having a mutation at
residue 307; designated At173 G307D; hypothetical 40 Met Thr Ser
Val Asn Val Lys Leu Leu Tyr Arg Tyr Val Leu Thr Asn 1 5 10 15 Phe
Phe Asn Leu Cys Leu Phe Pro Leu Thr Ala Phe Leu Ala Gly Lys 20 25
30 Ala Ser Arg Leu Thr Ile Asn Asp Leu His Asn Phe Leu Ser Tyr Leu
35 40 45 Gln His Asn Leu Ile Thr Val Thr Leu Leu Phe Ala Phe Thr
Val Phe 50 55 60 Gly Leu Val Leu Tyr Ile Val Thr Arg Pro Asn Pro
Val Tyr Leu Val 65 70 75 80 Asp Tyr Ser Cys Tyr Leu Pro Pro Pro His
Leu Lys Val Ser Val Ser 85 90 95 Lys Val Met Asp Ile Phe Tyr Gln
Ile Arg Lys Ala Asp Thr Ser Ser 100 105 110 Arg Asn Val Ala Cys Asp
Asp Pro Ser Ser Leu Asp Phe Leu Arg Lys 115 120 125 Ile Gln Glu Arg
Ser Gly Leu Gly Asp Glu Thr Tyr Ser Pro Glu Gly 130 135 140 Leu Ile
His Val Pro Pro Arg Lys Thr Phe Ala Ala Ser Arg Glu Glu 145 150 155
160 Thr Glu Lys Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr
165 170 175 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser
Ser Met 180 185 190 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val
Asn Thr Phe Lys 195 200 205 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu
Gly Gly Met Gly Cys Ser 210 215 220 Ala Gly Val Ile Ala Ile Asp Leu
Ala Lys Asp Leu Leu His Val His 225 230 235 240 Lys Asn Thr Tyr Ala
Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 Ile Tyr Ala
Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 Arg
Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280
285 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly
290 295 300 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp
Glu Asn 305 310 315 320 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp Ile
Thr Asp Val Ala Gly 325 330 335 Arg Thr Val Lys Lys Asn Ile Ala Thr
Leu Gly Pro Leu Ile Leu Pro 340 345 350 Leu Ser Glu Lys Leu Leu Phe
Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 Phe Lys Asp Lys Ile
Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375 380 Ile Asp His
Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp Val 385 390 395 400
Leu Glu Lys Asn Leu Ala Leu Ala Pro Ile Asp Val Glu Ala Ser Arg 405
410 415 Ser Thr Leu His Arg Phe Gly Asn Thr Ser Ser Ser Ser Ile Trp
Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu Ala Lys Gly Arg Met Lys Lys
Gly Asn Lys 435 440 445 Val Trp Gln Ile Ala Leu Gly Ser Gly Phe Lys
Cys Asn Ser Ala Val 450 455 460 Trp Val Ala Leu Asn Asn Val Lys Ala
Ser Thr Asn Ser Pro Trp Glu 465 470 475 480 His Cys Ile Asp Arg Tyr
Pro Val Lys Ile Asp Ser Asp Ser Gly Lys 485 490 495 Ser Glu Thr Arg
Val Gln Asn Gly Arg Ser 500 505 41 1521 DNA Artificial Sequence 5'
1197 bp from B. napus elongase KCS (SEQ ID NO3) and 3' 324 bp from
A. thaliana FAE1 (SEQ ID NO1) and having a mutation at nucleotide
position 920; designated Bn399 G307D; hypothetical 41 atg acg tcc
att aac gtt aag ctc ctt tac cat tac gtc ata acc aac 48 Met Thr Ser
Ile Asn Val Lys Leu Leu Tyr His Tyr Val Ile Thr Asn 1 5 10 15 ctt
ttc aac ctt tgc ttc ttt ccg tta acg gcg atc gtc gcc gga aaa 96 Leu
Phe Asn Leu Cys Phe Phe Pro Leu Thr Ala Ile Val Ala Gly Lys 20 25
30 gcc tat cgg ctt acc ata gac gat ctt cac cac tta tac tat tcc tat
144 Ala Tyr Arg Leu Thr Ile Asp Asp Leu His His Leu Tyr Tyr Ser Tyr
35 40 45 ctc caa cac aac ctc ata acc atc gct cca ctc ttt gcc ttc
acc gtt 192 Leu Gln His Asn Leu Ile Thr Ile Ala Pro Leu Phe Ala Phe
Thr Val 50 55 60 ttc ggt tcg gtt ctc tac atc gca acc cgg ccc aaa
ccg gtt tac ctc 240 Phe Gly Ser Val Leu Tyr Ile Ala Thr Arg Pro Lys
Pro Val Tyr Leu 65 70 75 80 gtt gag tac tca tgc tac ctt cca cca acg
cat tgt aga tca agt atc 288 Val Glu Tyr Ser Cys Tyr Leu Pro Pro Thr
His Cys Arg Ser Ser Ile 85 90 95 tcc aag gtc atg gat atc ttt tat
caa gta aga aaa gct gat cct tct 336 Ser Lys Val Met Asp Ile Phe Tyr
Gln Val Arg Lys Ala Asp Pro Ser 100 105 110 cgg aac ggc acg tgc gat
gac tcg tcg tgg ctt gac ttc ttg agg aag 384 Arg Asn Gly Thr Cys Asp
Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys 115 120 125 att caa gaa cgt
tca ggt cta ggc gat gaa act cac ggg ccc gag ggg 432 Ile Gln Glu Arg
Ser Gly Leu Gly Asp Glu Thr His Gly Pro Glu Gly 130 135 140 ctg ctt
cag gtc cct ccc cgg aag act ttt gcg gcg gcg cgt gaa gag 480 Leu Leu
Gln Val Pro Pro Arg Lys Thr Phe Ala Ala Ala Arg Glu Glu 145 150 155
160 acg gag caa gtt atc att ggt gcg cta gaa aat cta ttc aag aac acc
528 Thr Glu Gln Val Ile Ile Gly Ala Leu Glu Asn Leu Phe Lys Asn Thr
165 170 175 aac gtt aac cct aaa gat ata ggt ata ctt gtg gtg aac tca
agc atg 576 Asn Val Asn Pro Lys Asp Ile Gly Ile Leu Val Val Asn Ser
Ser Met 180 185 190 ttt aat cca act cca tcg ctc tcc gcg atg gtc gtt
aac act ttc aag 624 Phe Asn Pro Thr Pro Ser Leu Ser Ala Met Val Val
Asn Thr Phe Lys 195 200 205 ctc cga agc aac gta aga agc ttt aac ctt
ggt ggc atg ggt tgt agt 672 Leu Arg Ser Asn Val Arg Ser Phe Asn Leu
Gly Gly Met Gly Cys Ser 210 215 220 gcc ggc gtt ata gcc att gat cta
gca aag gac ttg ttg cat gtc cat 720 Ala Gly Val Ile Ala Ile Asp Leu
Ala Lys Asp Leu Leu His Val His 225 230 235 240 aaa aat acg tat gct
ctt gtg gtg agc aca gag aac atc act tat aac 768 Lys Asn Thr Tyr Ala
Leu Val Val Ser Thr Glu Asn Ile Thr Tyr Asn 245 250 255 att tac gct
ggt gat aat agg tcc atg atg gtt tca aat tgc ttg ttc 816 Ile Tyr Ala
Gly Asp Asn Arg Ser Met Met Val Ser Asn Cys Leu Phe 260 265 270 cgt
gtt ggt ggg gcc gct att ttg ctc tcc aac aag cct gga gat cgt 864 Arg
Val Gly Gly Ala Ala Ile Leu Leu Ser Asn Lys Pro Gly Asp Arg 275 280
285 aga cgg tcc aag tac gag cta gtt cac acg gtt cga acg cat acc gga
912 Arg Arg Ser Lys Tyr Glu Leu Val His Thr Val Arg Thr His Thr Gly
290 295 300 gct gac gac aag tct ttt cgt tgc gtg caa caa gga gac gat
gag aac 960 Ala Asp Asp Lys Ser Phe Arg Cys Val Gln Gln Gly Asp Asp
Glu Asn 305 310 315 320 ggc aaa atc gga gtg agt ttg tcc aag gac ata
acc gat gtt gct ggt 1008 Gly Lys Ile Gly Val Ser Leu Ser Lys Asp
Ile Thr Asp Val Ala Gly 325 330 335 cga acg gtt aag aaa aac ata gca
acg ttg ggt ccg ttg att ctt ccg 1056 Arg Thr Val Lys Lys Asn Ile
Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350 tta agc gag aaa ctt
ctt ttt ttc gtt acc ttc atg ggc aag aaa ctt 1104 Leu Ser Glu Lys
Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355 360 365 ttc aaa
gat aaa atc aaa cat tac tac gtc ccg gat ttc aaa ctt gct 1152 Phe
Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu Ala 370 375
380 att gac cat ttt tgt ata cat gcc gga ggc aga gcc gtg atc gat gag
1200 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val Ile Asp
Glu 385 390 395 400 cta gag aag aac tta gga cta tcg ccg atc gat gtg
gag gca tct aga 1248 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile Asp
Val Glu Ala Ser Arg 405 410 415 tca acg tta cat aga ttt ggg aat act
tca tct agc tca att tgg tat 1296 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 gaa tta gca tac ata gag
gca aag gga aga atg aag aaa ggg aat aaa 1344 Glu Leu Ala Tyr Ile
Glu Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 gct tgg cag
att gct tta gga tca ggg ttt aag tgt aat agt gcg gtt 1392 Ala Trp
Gln Ile Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460
tgg gtg gct cta cgc aat gtc aag gca tcg gca aat agt cct tgg caa
1440 Trp Val Ala Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp
Gln 465 470 475 480 cat tgc atc gat aga tat ccg gtt aaa att gat tct
gat ttg tca aag 1488 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp
Ser Asp Leu Ser Lys 485 490 495 tca aag act cat gtc caa aac ggt cgg
tcc taa 1521 Ser Lys Thr His Val Gln Asn Gly Arg Ser 500 505 42 506
PRT Artificial Sequence 5' 399 amino acids from B. napus elongase
KCS (SEQ ID NO3) and 3' 107 amino acids from A. thaliana FAE1 (SEQ
ID NO1) having a mutation at residue 306; designated Bn399 G307D;
hypothetical 42 Met Thr Ser Ile Asn Val Lys Leu Leu Tyr His Tyr Val
Ile Thr Asn 1 5 10 15 Leu Phe Asn Leu Cys Phe Phe Pro Leu Thr Ala
Ile Val Ala Gly Lys 20 25 30 Ala Tyr Arg Leu Thr Ile Asp Asp Leu
His His Leu Tyr Tyr Ser Tyr 35 40 45 Leu Gln His Asn Leu Ile Thr
Ile Ala Pro Leu Phe Ala Phe Thr Val 50 55 60 Phe Gly Ser Val Leu
Tyr Ile Ala Thr Arg Pro Lys Pro Val Tyr Leu 65 70 75 80 Val Glu Tyr
Ser Cys Tyr Leu Pro Pro Thr His Cys Arg Ser Ser Ile 85 90 95 Ser
Lys Val Met Asp Ile Phe Tyr Gln Val Arg Lys Ala Asp Pro Ser 100 105
110 Arg Asn Gly Thr Cys Asp Asp Ser Ser Trp Leu Asp Phe Leu Arg Lys
115 120 125 Ile Gln Glu Arg Ser Gly Leu Gly Asp Glu Thr His Gly Pro
Glu Gly 130 135 140 Leu Leu Gln Val Pro Pro Arg Lys Thr Phe Ala Ala
Ala Arg Glu Glu 145 150 155 160 Thr Glu Gln Val Ile Ile Gly Ala Leu
Glu Asn Leu Phe Lys Asn Thr 165 170 175 Asn Val Asn Pro Lys Asp Ile
Gly Ile Leu Val Val Asn Ser Ser Met 180 185 190 Phe Asn Pro Thr Pro
Ser Leu Ser Ala Met Val Val Asn Thr Phe Lys 195 200 205 Leu Arg Ser
Asn Val Arg Ser Phe Asn Leu Gly Gly Met Gly Cys Ser 210 215 220 Ala
Gly Val Ile Ala Ile Asp Leu Ala Lys Asp Leu Leu His Val His 225 230
235 240 Lys Asn Thr Tyr Ala Leu Val Val Ser Thr Glu Asn Ile Thr Tyr
Asn 245 250 255 Ile Tyr Ala Gly Asp Asn Arg Ser Met Met Val Ser Asn
Cys Leu Phe 260 265 270 Arg Val Gly Gly Ala Ala Ile Leu Leu Ser Asn
Lys Pro Gly Asp Arg 275 280 285 Arg Arg Ser Lys Tyr Glu Leu Val His
Thr Val Arg Thr His Thr Gly 290 295 300 Ala Asp Asp Lys Ser Phe Arg
Cys Val Gln Gln Gly Asp Asp Glu Asn 305 310 315 320 Gly Lys Ile Gly
Val Ser Leu Ser Lys Asp Ile Thr Asp Val Ala Gly 325 330 335 Arg Thr
Val Lys Lys Asn Ile Ala Thr Leu Gly Pro Leu Ile Leu Pro 340 345 350
Leu Ser Glu Lys Leu Leu Phe Phe Val Thr Phe Met Gly Lys Lys Leu 355
360 365 Phe Lys Asp Lys Ile Lys His Tyr Tyr Val Pro Asp Phe Lys Leu
Ala 370 375 380 Ile Asp His Phe Cys Ile His Ala Gly Gly Arg Ala Val
Ile Asp Glu 385 390 395 400 Leu Glu Lys Asn Leu Gly Leu Ser Pro Ile
Asp Val Glu Ala Ser Arg 405 410 415 Ser Thr Leu His Arg Phe Gly Asn
Thr Ser Ser Ser Ser Ile Trp Tyr 420 425 430 Glu Leu Ala Tyr Ile Glu
Ala Lys Gly Arg Met Lys Lys Gly Asn Lys 435 440 445 Ala Trp Gln Ile
Ala Leu Gly Ser Gly Phe Lys Cys Asn Ser Ala Val 450 455 460 Trp Val
Ala Leu Arg Asn Val Lys Ala Ser Ala Asn Ser Pro Trp Gln 465 470 475
480 His Cys Ile Asp Arg Tyr Pro Val Lys Ile Asp Ser Asp Leu Ser Lys
485 490 495 Ser Lys Thr His Val Gln Asn Gly Arg Ser 500 505 43 25
DNA Artificial Sequence primer for PCR 43 gcgctcgaaa atctattcaa
gaaca 25 44 33 DNA Artificial Sequence primer for PCR 44 gttcttgaat
agattttcga gcgcaccgat gat 33 45 28 DNA Artificial Sequence primer
for PCR 45 cggaacggca cgtgtgatga ttcgtcct 28 46 28 DNA Artificial
Sequence primer for PCR 46 aggacggatc atcacacgcg acgttccg 28 47 23
DNA Artificial Sequence primer for PCR 47 cccaaaccgg tttacctcgt tga
23 48 23 DNA Artificial Sequence primer for PCR 48 tcaacgaggt
aaaccggatt ggg 23 49 27 DNA Artificial Sequence primer for PCR 49
ccgcattgca gagttagtgt ctctaaa 27 50 27 DNA Artificial Sequence
primer for PCR 50 tttagagaca ctaactctgc aatgcgg 27 51 27 DNA
Artificial Sequence primer for PCR 51 ccaccgcatc tcagagttag tgtctct
27 52 27 DNA Artificial Sequence primer for PCR 52 agagacacta
actctgagat gcggtgg 27 53 33 DNA Artificial Sequence primer for PCR
53 ggggatccat gacgtccgtt aacgttaagc tcc 33 54 31 DNA Artificial
Sequence primer for PCR 54 ccgaattctt aggaccgacc gttttggaca c 31 55
33 DNA Artificial Sequence primer for PCR 55 ggggatccat gacgtccatt
aacgtaaagc tcc
33 56 38 DNA Artificial Sequence primer for PCR 56 ccgaattctt
aggaccgacc gttttggaca tgagtctt 38
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