U.S. patent application number 10/615262 was filed with the patent office on 2004-06-03 for medicament comprising hgf gene.
This patent application is currently assigned to MedGene Bioscience, Inc.. Invention is credited to Morishita, Ryuichi, Nakamura, Toshikazu, Ochi, Takahiro, Ogihara, Toshio, Tomita, Tetsuya.
Application Number | 20040105882 10/615262 |
Document ID | / |
Family ID | 26399523 |
Filed Date | 2004-06-03 |
United States Patent
Application |
20040105882 |
Kind Code |
A1 |
Morishita, Ryuichi ; et
al. |
June 3, 2004 |
Medicament comprising HGF gene
Abstract
The present invention relates to a medicament comprising a HGF
gene. The medicament of the present invention may be topically
applied to the target organs so that the effects can be selectively
exhibited, resulting in minimizing the side effects of HGF.
Inventors: |
Morishita, Ryuichi; (Osaka,
JP) ; Ogihara, Toshio; (Osaka, JP) ; Nakamura,
Toshikazu; (Osaka, JP) ; Tomita, Tetsuya;
(Osaka, JP) ; Ochi, Takahiro; (Kobe-shi,
JP) |
Correspondence
Address: |
SUGHRUE MION, PLLC
2100 PENNSYLVANIA AVENUE, N.W.
SUITE 800
WASHINGTON
DC
20037
US
|
Assignee: |
MedGene Bioscience, Inc.
|
Family ID: |
26399523 |
Appl. No.: |
10/615262 |
Filed: |
July 9, 2003 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10615262 |
Jul 9, 2003 |
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09660522 |
Sep 12, 2000 |
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09660522 |
Sep 12, 2000 |
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09029497 |
Jun 9, 1998 |
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6248722 |
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09029497 |
Jun 9, 1998 |
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PCT/JP96/02359 |
Aug 22, 1996 |
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Current U.S.
Class: |
424/450 ;
424/93.21; 435/458; 514/44R |
Current CPC
Class: |
A61K 9/1271 20130101;
A61P 9/00 20180101; A61P 9/10 20180101; A61K 9/1272 20130101; A61K
48/00 20130101; A61K 38/1833 20130101 |
Class at
Publication: |
424/450 ;
424/093.21; 514/044; 435/458 |
International
Class: |
A61K 048/00; A61K
009/127; C12N 015/88 |
Foreign Application Data
Date |
Code |
Application Number |
Aug 29, 1995 |
JP |
07-245475 |
Feb 20, 1996 |
JP |
08-058467 |
Claims
What is claimed is:
1. A medicament comprising a HGF gene.
2. A liposome containing a HGF gene.
3. A liposome according to claim 2, which is a membrane fusion
liposome fused to Sendai virus.
4. A medicament comprising a liposome according to claim 2 or
3.
5. A medicament according to claim 1 or 4, which is for use in the
treatment for arterial diseases.
6. A medicament according to claim 1 or 4, which is for use in the
treatment for cartilage injury.
Description
TECHNICAL FIELD TO WHICH THE INVENTION PERTAINS
[0001] The present invention relates to a medicament for use in the
gene therapy and the like. More particularly, the present invention
relates to a medicament comprising a hepatocyte growth factor (HGF)
gene as well as a liposome containing the HGF gene.
PRIOR ARTS
[0002] HGF is a physiologically active peptide that exhibit diverse
pharmacological activities. The pharmacological activities of HGF
are described in, e.g., JIKKEN-IGAKU (Experimental Medicine), Vol.
10, No. 3 (extra issue), 330-339 (1992). In view of its
pharmacological activities, HGF is expected to be useful as a drug
for the treatment of liver cirrhosis or renal diseases; epithalial
cell growth accelerators; anticancer agents; agents for the
prevention of side effects in cancer therapy; agents for the
treatment of lung disorders, gastrointestinal damages or cranial
nerve disorders; agents for the prevention of side effects in
immunosuppression; collagen degradation accelerators; agents for
the treatment of cartilage disorders, arterial diseases, pulmonary
fibrosis, hepatic diseases, blood coagulopathy, plasma
hypoproteinosis or wounds; agents for the improvement of nervous
disorders; hematopoietic stem cell potentiators; and hair growth
promoters (Japanese Patent KOKAI (Laid-Open) Nos. 4-18028 and
4-49246, EP 492614, Japanese Patent KOKAI (Laid-Open) No. 6-25010,
WO 93/8821, Japanese Patent KOKAI (Laid-Open) Nos. 6-172207,
7-89869 and 6-40934, WO 94/2165, Japanese Patent KOKAI (Laid-Open)
Nos. 6-40935, 6-56692 and 7-41429, WO 93/3061, Japanese Patent
KOKAI (Laid-Open) No. 5-213721, etc.).
[0003] As to gene therapy, extensive studies and investigations
have been currently made all over the world for adenosine deaminase
deficiency, AIDS, cancer, pustulous fibrosis or hemophilia,
etc.
[0004] However, gene therapy using the HGF genes is unknown yet. It
is even unclear if such gene therapy is effectively applicable.
[0005] Problems to be Solved by the Invention
[0006] HGF is known to be one of the drugs that have a short half
life in blood. As a natural consequence, persistent topical
administration has been desired for HGF.
[0007] In view of the diverse pharmacological activities of HGF,
HGF is expected to be developed as a drug having extended
applications to various diseases. On the other hand, when HGF is
systemically administered, side effects might be caused due to the
diverse pharmacological activities of HGF. In addition, when HGF
itself is intravenously administered, HGF encounters such a
drawback that a considerable amount of HGF is retained in the
liver, resulting in reduction of the amount of HGF to reach the
target organ.
[0008] Means for Solving the Problems
[0009] The present invention has been made to solve the foregoing
problems. In summary, the present invention relates to:
[0010] (1) a medicament comprising a HGF gene;
[0011] (2) a liposome containing the HGF gene;
[0012] (3) a liposome according to (2), which is a membrane fusion
liposome fused to Sendai virus; (4) a medicament comprising the
liposome according to (2) or (3); (5) a medicament according to (1)
or (4), for use in the treatment for arterial disorders; and, (6) a
medicament according to (1) or (4), for use in the treatment for
cartilage injuries.
BRIEF DESCRIPTION OF THE DRAWINGS
[0013] FIG. 1 shows an expression of HGF in rat coronary
endothelial cells sensitized with the hemagglutinating virus of
Japan (HVJ)-liposome-DNA in Test Example 1.
[0014] In FIG. 2, the (line) graph shows a cell growth rate in the
presence or absence of HGF from the HVJ-liposome-cont-sensitized
endothelial cells in Test Example 2, wherein "DSF" designates a
group of the endothelial cells sensitized with HVJ-liposome-cont
and "HGF" designates a group incubated in the presence of
recombinant human HGF in a predetermined concentration. The bar in
FIG. 2 shows a cell growth rate of the HVJ-liposome-DNA-sensitized
endothelial cells in Test Example 2, wherein "DSF" designates a
group of the endothelial cells sensitized with HVJ-liposome-cont
and "HGF vector" designates a group of the endothelial cells
sensitized with HVJ-liposome-DNA.
[0015] FIG. 3 shows a cell growth rate of endothelial cells
sensitized with HVJ-liposome-DNA in the presence or absence of
anti-HGF antibody in Test Example 2, wherein "control" represents a
group of the HVJ-liposome-cont-sensitized endothelial cells
incubated in the presence of IgG control; "HGF" represents a group
of the HVJ-liposome-DNA-sensitiz- ed endothelial cells incubated in
the presence of IgG control; and "HGFab" represents a group of the
HVJ-liposome-DNA-sensitized endothelial cells incubated in the
presence of a rabbit anti-human HGF antibody. The cell growth rate
(%) is expressed in terms of relative % when the growth rate in the
control group is made 100.
[0016] FIG. 4 is a graph showing the cell growth effect of the
culture supernatant from the HVJ-liposomeDNA-sensitized rat
vascular smooth muscle cells (hereinafter often abbreviated as
VSMCs) on rat coronary endothelial cells in Test Example 3, wherein
"control" designates a group added with the culture supernatant
from the HVJ-liposome-cont-sensitized rat VSMCs, and "HGF"
designates a group added with the culture supernatant from the
HVJ-liposome-DNA-sensitized rat VSMCs.
[0017] FIG. 5 is a graph showing the results in Test Example 3 in
which the concentration of HGF in the supernatant from the
incubated HVJ-liposome-DNA-sensitized rat VSMCs was determined
using the anti-human HGF antibody. In the figure, "no-treatment"
represents a group of the culture supernatant of non-sensitized
VSMCs; "control" represents a group of the supernatant from the
incubated HVJ-liposome-cont-sensitized rat VSMCs; and "HGF"
represents a group of the supernatant from the incubated
HVJ-liposome-DNA-sensitized rat VSMCs.
[0018] FIG. 6 is a graph showing the results in Test Example 3 in
which the concentration of HGF in the supernatant from the
incubated HVJ-liposome-DNA-sensitized rat VSMCs was determined
using the anti-rat HGF antibody. In the figure, "no-treatment"
represents a group of the culture supernatant of non-sensitized
VSMCs; "control" designates a group of the supernatant from the
incubated HVJ-liposome-cont-sensitized rat VSMCs; and "HGF"
designates a group of the supernatant from the incubated
HVJ-liposome-DNA-sensitized rat VSMCs.
[0019] FIG. 7 is a graph showing the cell growth effect of the
supernatant from the incubated HVJ-liposome-DNA-sensitized rat
coronary endothelial cells on rat coronary endothelial cells in
Test Example 4, wherein A, B and C designate, respectively, a group
added with the supernatant of the incubated
HVJ-liposome-DNA-sensitized rat coronary endothelial cells, a group
added with the supernatant of the incubated
HVJ-liposome-cont-sensi- tized rat coronary endothelial cells, and
a group of no-treatment animals.
[0020] FIG. 8 shows the cell growth effect of
HVJ-liposome-DNA-sensitized rat coronary endothelial cells on rat
coronary endothelial cells in the presence of an anti-HGF antibody
in Test Example 4. In the figure, A represents a group added with
the supernatant from the incubated HVJ-liposome-DNA-sensitized rat
coronary endothelial cells; B represents a group added with the
supernatant from the incubated HVJ-liposome-cont-sensitized rat
coronary endothelial cells; C represents a group added with the
anti-HGF antibody to the supernatant from the incubated
HVJ-liposome-DNA-sensitized rat coronary endothelial cells; and D
represents a group added with the control antibody to the
supernatant of the incubated HVJ-liposome-DNA-sensitized rat
coronary endothelial cells.
[0021] FIG. 9 is a drawing showing the cell growth of endothelial
cells in Test Example 5 when HVJ-liposomeDNA-sensitized human VSMCs
were co-incubated with non-sensitized human endothelial cells. In
the figure, "control" represents a group co-incubated with
HVJ-liposome-cont-sensitiz- ed VSMCs, and "HGF" represents a group
of the supernatant from the incubated HVJ-liposome-DNA-sensitized
VSMCs.
[0022] FIG. 10 indicates the cell growth of endothelial cells in
Test Example 6 when HVJ-liposomeDNA-sensitized rat VSMCs were
co-incubated with non-sensitized rat coronary endothelial cells. In
the figure, "control" represents a group co-incubated with the
HVJ-liposome-cont-sensitized VSMCs, and "HGF" represents a group of
the culture supernatant of the HVJ-liposome-DNA-sensitized
VSMCs.
[0023] FIG. 11 shows an increase in the number of minute blood
vessels in rat heart muscle directly injected with HVJ-liposome-DNA
in Test Example 8, wherein "HGF" denotes the number of minute blood
vessels in rat heart muscle directly injected with
HVJ-liposome-DNA, and "control" denotes the number of minute blood
vessels in rat heart muscle directly injected with
HVJ-liposome-cont.
[0024] FIG. 12 is a drawing showing that 3 weeks after
administration of HVJ-liposome-DNA into the joint, development of
cartilage-like cells was noted in Test Example 9, in which the
synthesis of Toluidine Blue-stained proteoglycan was observed.
[0025] FIG. 13 is a drawing showing that 4 weeks after
administration of HVJ-liposome-DNA into the joint, development of
cartilage-like cells was noted in Test Example 9, in which the
synthesis of Toluidine Blue-stained proteoglycan was observed.
[0026] FIG. 14 is a drawing showing that even 4 weeks after
administration of HVJ-liposome-DNA (TGF-.beta.) prepared in
Comparative Example 2 into the joint, such development of
cartilage-like cells as observing-Toluidine Blue-stained
proteoglycan synthesis was not noted in Test Example 9.
[0027] FIG. 15 is a drawing showing that even 4 weeks after
administration of HVJ-liposome-cont prepared in Comparative Example
1 into the joint, no such development of cartilage-like cells as
observing Toluidine Blue-stained proteoglycan synthesis was noted
in Test Example 9.
BEST MODE FOR CARRYING OUT THE INVENTION
[0028] The "HGF gene" employed in the present invention indicates a
gene capable of expressing HGF. Thus, so long as a polypeptide
expressed has substantially the same effect as that of HGF, the HGF
gene may have a partial deletion, substitution or insertion of the
nucleotide sequence, or may have other nucleotide sequence ligated
therewith at the 5'-terminus and/or 3'-terminus thereof. Typical
examples of such HGF genes include HGF genes as described in
Nature, 342, 440 (1989), Japanese Patent KOKAI (Laid-Open) No.
5-111383, Biohem. Biophys. Res. Commun., 163, 967 (1989), etc.
These genes may be used in the present invention.
[0029] The HGF gene is incorporated into an appropriate vector and
the HGF gene-bearing vector is provided for use. For example, the
HGF gene may be used in the form of a viral vector having the HGF
gene as described hereinafter, or in the form of an appropriate
expression vector having the HGF gene.
[0030] The "pharmaceutical composition" used in the present
invention indicates a medicament for the treatment or prevention of
human diseases, which is attributed to the pharmacological
activities of HGF. For example, exemplified are medicaments for the
treatment or prevention of the diseases given hereinabove.
[0031] According to the present invention, the HGF gene is
introduced into cells wherein HGF is expressed in those cells to
exhibit the pharmacological actions. Thus, the medicament of the
present invention is effectively applicable to the diseases for
which HGF itself is effective.
[0032] Where the HGF gene is introduced into, e.g., cells, the
growth of vascular endothelial cells is accelerated, while
undesired growth of vascular smooth muscle cells is not
accelerated, as demonstrated in the Examples hereinafter. Moreover,
as demonstrated in the Example hereinafter, where the HGF gene is
introduced into the heart in vivo animal test using rats,
angiogenesis is observed. Therefore, the HGF gene is effective for
the treatment and prevention of arterial disorders, in particular,
various diseases caused by disturbance which mainly involves
abnormal proliferation of vascular smooth muscle cells (e.g.,
restenosis after percutaneous transluminal coronary angioplasty
(PTCA), arteriosclerosis, insufficiency of peripheral circulation,
etc.), and for the treatment and prevention of diseases such as
myocardial infarction, myocardia, peripheral angiostenosis, cardiac
insufficiency, etc. HGF itself is also useful for the treatment and
prevention of the diseases as described above, since HGF promotes
the proliferation of vascular endothelial cells but does not
promote the growth of vascular smooth muscle cells. The
pharmacological effects of the HGF gene are attributed to those of
HGF itself.
[0033] As demonstrated in the Examples hereinafter, introduction of
the HGF gene into the joint results in promoting repair of
articular cartilage cells thereby to promote the proliferation of
proteoglycan-synthesizing cells. Therefore, the HGF gene is
effective for the prevention and treatment of various cartilage
injuries such as osteogenetic abnormality, arthritis deformans,
discopathy deformans, fracture repair and restoration
insufficiency, trauma caused by sporting, key puncher's disease,
etc. HGF itself is useful for the treatment and prevention of the
diseases described above, since HGF promotes repair and growth of
cartilage cells. The effects of the HGF gene are based on those of
HGF itself.
[0034] "Liposome" is a closed vesicle of lipid bilayer
encapsulating an aqueous compartment therein. It is known that the
lipid bilayer membrane structure is extremely similar to biological
membranes. To prepare the liposomes of the present invention,
phospholipids are employed. Typical examples of phospholipids are
phosphatidylcholines such as lecithin, lysolecithin, etc.; acidic
phospholipids such as phosphatidylserine, phosphatidylglycerol,
phosphatidylinositol, phosphatidylic acid, etc.; or phospholipids
obtained by replacing an acyl group(s) of these acidic
phospholipids with lauroyl, myristoyl, oleoyl, etc.; and
sphingophospholipids such as phosphatidylethanolamine,
sphingomyelin, etc. Neutral lipids such as cholesterol may also be
added to these phospholipids. The liposomes may be prepared, in a
conventional manner, from naturally occurring materials such as
lipids in normal cell membranes. The liposomes containing the HGF
gene of the present invention may be prepared, for example, by
suspending a thin layer of purified phospholipids in a solution
containing the HGF gene and then treating the suspension in a
conventional manner such as ultra-sonication.
[0035] The liposomes containing the HGF gene of the present
invention may be appropriately fused to viruses, etc. to form
membrane fusion liposomes. In this case, it is preferred to
inactivate viruses, e.g., through ultraviolet irradiation, etc. A
particularly preferred example of the membrane fusion liposome is a
membrane fusion liposome fused with Sendai virus (hemagglutinating
virus of Japan: HVJ). The membrane fusion liposome may be produced
by the methods as described in NIKKEI Science, April, 1994, pages
32-38; J. Biol. Chem., 266 (6), 3361-3364 (1991), etc. In more
detail, the HVJ-fused liposome (HVJ-liposome) may be prepared,
e.g., by mixing purified HVJ inactivated by ultraviolet
irradiation, etc. with a liposome suspension containing the HGF
gene vector, gently agitating the mixture and then removing unbound
HVJ by sucrose density gradient centrifugation. The liposomes may
be bound to substances having an affinity to target cells, thereby
to enhance an efficiency of gene introduction into the target
cells. Examples of the substances having an affinity to the target
cells include ligands such as an antibody, a receptor, etc.
[0036] For introduction of the HGF gene into cells, conventional
methods are employed, which are roughly classified into
introduction via viral vectors and other strategies (NIKKEI
Science, April, 1994, pages 20-45; GEKKAN YAKUJI, 36 (1), 23-48
(1994) and references cited therein). Both methods are available
for the preparation of the medicament of the present invention.
[0037] The former method using viral vectors comprises the step of
incorporating the HGF gene into, e.g., a retrovirus, an adenovirus,
an adeno-related virus, a herpes virus, a vaccinia virus, a
poliovirus, a sindbis virus or other RNA viruses. Of these viruses,
a retrovirus, an adenovirus and an adeno-related virus are
particularly preferably employed for the introduction.
[0038] Examples of the other methods include the liposome method,
lipofectin method, microinjection method, calcium phosphate method,
electroporation method. Of these methods, particularly preferred is
the liposome method.
[0039] For practical use of the HGF gene as a medicament, it is
advantageous to introduce the HGF directly into the body (in vivo
method). Alternatively, certain cells are collected from human, the
HGF gene is then introduced into the cells outside the body and the
HGF gene-introduced cells are returned to the body (ex vivo
method). These methods are described in NIKKEI Science, April,
1994, pages 20-45; GEKKAN-YAKUJI, 36 (1), 23-48 (1994) and
references cited therein. Any of these methods are suitably chosen
depending upon a disease to be treated, target organs, etc. and
applied to the medicament compositions of the present
invention.
[0040] The in vivo method is less costly, less laborious and
therefore more convenient than the ex vivo method, but the latter
method provides a higher efficiency of introduction of the HGF gene
into cells.
[0041] Where the medicament of the present invention is
administered by the in vivo method, the medicament may be
administered through any route appropriate for diseases to be
treated, target organs, etc. The medicament may be administered
intravenously, intraarterially, subcutaneously, intramuscularly,
etc., or directly to the objective organ of diseases, e.g., kidney,
liver, lung, brain, nerve, etc. Direct administration to the
objective site can treat the target organ selectively. For example,
in gene therapy using a gene for restenosis after PTCA, the
composition may be administered intraarterially (JIKKEN-IGAKU, 12
(extra issue 15), 1298-1933 (1994). Preferably, the medicament of
the present invention is applied at the tip of a balloon used for
PTCA and rubbed the tip against blood vessel, whereby the
medicament may be introduced directly into vascular endothelial
cells and vascular smooth muscle cells.
[0042] Where the ex vivo method as described above is used to
introduce the HGF gene, human cells (e.g., lymphocytes or
hematopoietic stem cells) are harvested in a conventional manner
and the harvested cells are sensitized with the medicament of the
present invention for gene introduction. Thereafter the
HGF-producing cells are inserted back to human.
[0043] Where the medicament is administered by the in vivo method,
the medicament may take various preparation forms, including the
form of liquid preparation. In general, the medicament may be
preferably prepared into an injection comprising the HGF gene as an
active ingredient. If necessary and desired, conventional carriers
may be added to the composition. The injection may be prepared in a
conventional manner, e.g., by dissolving the HGF gene in an
appropriate solvent (e.g., sterilized water, a buffered solution, a
physiological saline solution, etc.), filtering the solution
through a filter, etc. for sterilization, filling up the solution
in a sterile container. The medicament may be prepared using the
HGF gene-incorporated viral vector, instead of the HGF gene itself.
Where the liposomes containing the HGF gene embedded therein (or
HVJ-liposomes) are employed, the medicament may be in the form of
liposome preparations such as a suspension, a frozen preparation, a
centrifugally concentrated frozen preparation, etc.
[0044] The content of the HGF gene in the medicament may be
appropriately varied depending upon diseases to be treated, target
organs, patients' ages or body weights, etc. However, it is
appropriate to administer in a dose of 0.0001 mg to 100 mg,
preferably 0.001 mg to 10 mg when calculated as the HGF gene. The
dose may be divided into several days or a few months.
EXAMPLES
[0045] Hereinafter the present invention will be described in more
detail with reference to the examples but is not deemed to be
limited thereto. Materials and methods used in the following
examples are outlined below.
[0046] Materials and Methods
[0047] (1) HGF Expression Vector
[0048] The HGF expression vector was prepared by inserting human
HGF cDNA (2.2 kb, Biochem. Biophys. Res. Commun., 172, 321-327
(1990); Japanese Patent KOKAI (Laid-Open) No. 5-111383) between the
EcoRI and NotI sites of pUC-SR.alpha. expression vector (FEBS, 333,
61-66 (1993)). In this plasmid vector, transcription of HGF cDNA is
regulated by SRa promoter (Nature, 342, 440-443 (1989)).
[0049] (2) Cell culture
[0050] Rat coronary endothelial cells were isolated from the
enzymatically digested heart of 8 weeks aged Sprague-Dawley (SD)
rats by density gradient centrifugation (Transplantation, 57,
1653-1660 (1994)). Rat aortic vascular smooth muscle cells (VSMCs)
were obtained from 12 weeks aged SD rats by enzymatic treatment (J.
Clin. Invest., 93, 355-360 (1994)). These cells were maintained in
DMEM medium supplemented with 10% (vol/vol) calf fetal serum,
penicillin (100 U/ml) and streptomycin (100 .mu.g/ml). The cells
were incubated at 37.degree. C. in a humidified 95% air-5% CO.sub.2
atmosphere. The culture medium was routinely changed at 2
day-intervals. Both immunopathological and morphological
observation revealed that these cells were endothelial cells and
smooth muscle cells, respectively.
[0051] Human aortic endothelial cells (five passages) and human
VSMCs (five passages) were obtained from Kurabo Co. The endothelial
cells were incubated in a manner similar to the above method in
MCDB131 medium supplemented with 5% calf fetal serum, epidermal
growth factor (10 ng/ml), basic fibroblast growth factor (2 ng/ml)
and dexamethasone (1 .mu.M).
[0052] Endothelial cells in the stationary state were prepared
according to the method described in J. Clin. Invest., 86,
1690-1697 (1990), ibid., 94, 824-829 (1994).
[0053] (3) Transfection of the HGF Gene into HVJ-Liposomes In
Vitro
[0054] Endothelial cells or VSMCs were inoculated for sensitization
on a 6-well plate in a cell count of 10.sup.6 and proliferated to
reach 80% confluence. The cells were washed 3 times with a balanced
salt solution (137 mM NaCl, 5.4 mM KCl, 10 mM Tris-HCl, pH 7.6;
hereinafter abbreviated as "BSS") supplemented with 2 mM calcium
chloride. To the cells was added 1 ml of a solution of the
HVJ-liposome-DNA (containing 2.5 mg of lipids and 10 mg of the
embedded DNA) obtained in Example 1 hereinafter or 1 ml of a
solution of the HVJ-liposome-cont obtained in Comparative Example 1
hereinafter. The resulting mixture was incubated at 4.degree. C.
for 5 minutes and at 37.degree. C. for further 30 minutes. The
cells were washed and maintained in a fresh medium containing 10%
bovine serum in a CO.sub.2 incubator.
[0055] (4) Assay for the HGF Concentrations in Endothelial Cells
and VSMCs
[0056] The concentration of HGF produced from the sensitized
endothelial cells and VSMCs was assayed by ELISA. That is, rat or
human endothelial cells or VSMCs were inoculated on a 6-well plate
(made by Corning) in a cell density of 5.times.10.sup.4
cells/cm.sup.2, followed by incubation for 24 hours. The medium was
replenished 24 hours after the sensitization, and incubation was
continued for further 48 hours. To investigate if HGF was released,
the sensitized cells (48 hours after sensitization) were washed and
added to 1 ml of a serum-free medium containing 5.times.10.sup.-7 M
insulin, 5 .mu.g/ml transferrin and 0.2 mM ascorbate. After 24
hours, the culture media were collected, centrifuged at 600 g for
10 minutes and then stored at -20.degree. C.
[0057] The HGF concentration in the media was determined by an
enzyme immunoassay using an anti-rat HGF antibody or an anti-human
HGF antibody (Exp. Cell Res., 210, 326-335 (1994); Jpn. J. Cancer
Res., 83, 1262-1266 (1992)). A rabbit anti-rat or an anti-human HGF
IgG was coated onto a 96-well plate (made by Corning) at 4.degree.
C. for 15 hours. After blocking with 3% bovine serum
albumin-containing PBS (phosphate buffered saline), the culture
medium was added to each well, and incubation was performed at
25.degree. C. for 2 hours. After washing each well 3 times with PBS
containing 0.025% Tween (PBS-Tween), a biotinated rabbit anti-rat
HGF IgG or an anti-human HGF IgG was added to each well followed by
incubation at 25.degree. C. for 2 hours. After washing with
PBS-Tween, each well was incubated together with horse radish
peroxidase-bound streptoavidin-biotin complex (PBS-Tween solution).
The enzymatic reaction was initiated by adding thereto a substrate
solution (containing 2.5 mM o-phenylenediamine, 100 mM sodium
phosphate, 50 mM citrate and 0.015% hydrogen peroxide). The
reaction was terminated by adding 1 M sulfuric acid to the system.
Absorbance was measured at 490 nm. The anti-human HGF antibody is
cross-reactive only with human HGF but not with rat HGF. The
anti-rat HGF antibody is cross-reactive solely with rat HGF but not
with human HGF.
[0058] (5) HGF
[0059] The human and rat recombinant HGFs employed were purified
from the culture solution of CHO cells or C-127 cells transfected
with an expression plasmid bearing human or rat HGF cDNA (Cell, 77,
261-271 (1994); J. Clin. Invest., 93, 355-360 (1994)).
[0060] (6) Statistical Analysis
[0061] All runs were repeated at least 3 times. Data measured are
shown by mean.+-.standard error. Statistical analysis of the
measured data was made according to the Duncan's test.
[0062] (7) Hematoxylin-Eosin (HE) Staining and Azan Staining
[0063] Ten days after the gene introduction, the HGF
gene-introduced rats were sacrificed by perfusion with heparinized
physiological saline. Fixation was then made overnight with a 4%
paraformaldehyde PBS solution. After fixation, the tissue was
embedded in paraffin. Slides were prepared and stained with HE and
Azan in a conventional manner. The slides were examined on a
microscope to count the number of microvessels.
Example 1
[0064] Preparation of HVJ-Liposomes Containing the HGF Expression
Vector
[0065] Phosphatidylserine, phosphatidylcholine and cholesterol were
mixed with tetrahydrofuran in a weight ratio of 1:4.8:2. By
distilling tetrahydrofuran off through a rotary evaporator, the
lipid mixture (10 mg) was precipitated onto the container wall.
After 96 .mu.g of high mobility group (HMG) 1 nuclear protein
purified from bovine thymus was mixed with a BBS (200 .mu.l)
solution of plasmid DNA (300 .mu.g) at 20.degree. C. for an hour,
the mixture was added to the lipid mixture obtained above. The
resulting liposome-DNA-HMG 1 complex suspension was mixed with a
vortex, ultrasonicated for 3 seconds and then agitated for 30
minutes.
[0066] The purified HVJ (strain Z) was inactivated by UV
irradiation (110 erg/mm.sup.2 sec) for 3 minutes immediately before
use. BSS was added to and mixed with the liposome suspension (0.5
ml, containing 10 mg of the lipids) obtained above and HVJ (20,000
hemagglutinating units) to make the whole volume 4 ml. The mixture
was incubated at 4.degree. C. for 10 minutes and gently agitated at
37.degree. C. for further 30 minutes. The unreacted HVJ was removed
from the HVJ-liposomes by sucrose density gradient centrifugation.
That is, the upper layers in the sucrose density gradient were
collected to give the HVJ-liposomes containing the HGF expression
vector (containing 10 .mu.g/ml of the HGF expression vector). The
HVJ-liposomes containing the HGF expression vector is hereinafter
often referred to as HVJ-liposome-DNA.
Example 2
[0067] Administration of the HVJ-Liposome Containing the HGF
Expression Vector to Rats
[0068] The HVJ-liposomes containing the HGF expression vector were
prepared by the method as described in the above Example, using 64
.mu.g of HMG 1 nuclear protein and 200 .mu.g of plasmid DNA. BSS
was added to and mixed with the liposome suspension (0.5 ml,
containing 10 mg of the lipids) and HVJ (35,000 hemagglutinating
units) to make the whole volume 2 ml.
[0069] SD rats (weighing 400-500 g; purchased from Japan Charles
River) were anesthetized with intraperitoneal administration of
sodium pentobarbital (0.1 ml/100 mg), warmed and maintained
breathing by an automated breather. The rats were subjected to
thoracotomy at the left side. The HVJ-liposome-DNA or
HVJ-liposome-cont (20 .mu.l) was carefully injected directly
through the cardiac apex using a 30 G syringe.
Comparative Example 1
[0070] Preparation of HVJ-Liposomes Containing no HGF Expression
Vector
[0071] A vector bearing no HGF gene was treated in the same manner
as described in Example 1 to prepare the HVJ-liposomes containing
no HGF expression vector. The HGF expression vector-free
HVJ-liposomes are hereinafter referred to as HVJ-liposome-cont.
Comparative Example 2
[0072] Preparation of HVJ-Liposomes Containing Human TGF-.beta.
Expression Vector
[0073] HVJ-liposomes containing human TGF-.beta. expression vector
were prepared in a manner similar to Example 1 except for using
human TGF-.beta. expression vector.
[0074] The HVJ-liposomes containing human TGF-expression vector are
hereinafter referred to as HVJ-liposome-DNA (TGF-.beta.).
Test Example 1
[0075] Expression of HGF in Rat Coronary Endothelial Cells
Sensitized with HVJ-Liposome-DNA
[0076] HVJ-liposome-DNA (concentration of the HGF expression vector
in liposomes: 10 .mu.g/ml) was sensitized to rat coronary
endothelial cells (cell count: 10.sup.6). HGF production was
determined by ELISA. For control, a similar test was conducted
using HVJ-liposome-cont. HGF production was also determined on the
non-sensitized rat coronary endothelial cells (no-treatment group).
The results are shown in FIG. 1 (n=6), wherein "HGF" represents the
group of rat coronary endothelial cells sensitized with
HVJ-liposome-DNA.
[0077] As shown in FIG. 1, the rat coronary endothelial cells
sensitized with HVJ-liposome-DNA produced and secreted HGF on a
high level. On the other hand, HGF production was not substantially
observed either in the intact group or in the group of the rat
coronary endothelial cells sensitized with HVJ-liposome-cont.
[0078] Cell counting in the groups tested reveals that the HGF
expression group showed significantly high cell counts.
Test Example 2
[0079] Effects of the Sensitized HGF Expression Vector on
Proliferation of Endothelial Cells
[0080] Human endothelial cells were sensitized with
HVJ-liposome-cont. The sensitized cells were incubated in the
presence or absence of added exogenously recombinant human HGF (1,
10 and 100 ng/ml) and a cell growth rate (%) was determined. The
results are shown in FIG. 2 ((line) graph, n=6), wherein "DSF"
represents the group of the endothelial cells sensitized with
HVJ-liposome-cont and "HGF" represents shows the group incubated in
the presence of recombinant human HGF in a definite concentration
(*: P<0.05, **: P<0.01 for DSF).
[0081] The (line) graph shown in FIG. 2 reveals that the growth of
endothelial cells is promoted by the exogenously added HGF.
[0082] The endothelial cells sensitized with HVJ-liposome-DNA
(concentration: 10 .mu.g/ml) were similarly incubated, and the
increased cells were counted to determine a cell growth rate (%).
For control, the endothelial cells sensitized with
HVJ-liposome-cont were also incubated, and the increased cells were
counted to determine a cell growth rate (%). The results are shown
in FIG. 2 (bar, n=6), wherein "DSF" designates the group of the
endothelial cells sensitized with HVJ-liposome-cont, and "HGF"
designates the group of the endothelial cells sensitized with
HVJ-liposome-DNA (**: P<0.01 for DSF, #: P<0.05 for HGF, 100
ng/ml).
[0083] As is noted from the bar shown in FIG. 2, the results reveal
that the cell growth rate of the HVJ-liposome-DNA-sensitized
endothelial cells is markedly higher than that of the control group
and significantly high even when compared to that of the
exogenously added HGF.
[0084] The aforesaid endothelial cells sensitized with
HVJ-liposome-DNA were incubated in the presence or absence of a
rabbit anti-human HGF antibody. The cells increased were counted to
determine a cell growth rate. For control, the endothelial cells
sensitized with HVJ-liposome-cont were incubated, and the cells
increased were counted in a similar manner to determine a cell
growth rate. The rabbit anti-human HGF antibody (10 .mu.g/ml) was
purified by the method as described in Jpn. J. Cancer Res., 83,
1262-1266 (1992). This antibody is capable of neutralizing the
biological activity of 10 ng/ml in its concentration of 10
.mu.g/ml. The anti-human HGF antibody is cross-reactive only with
human HGF but not with rat HGF, whereas the anti-rat HGF antibody
is cross-reactive only with rat HGF but not with human HGF. Normal
rabbit serum IgG (10 .mu.g/ml) was used for control.
[0085] The results are shown in FIG. 3 (n=6), wherein "control"
designates the group of HVJ-liposome-cont-sensitized endothelial
cells incubated in the presence of IgG control; "HGF" designates
the group of HVJ-liposome-DNA-sensitized endothelial cells
incubated in the presence of IgG control; and "HGFab" designates
the group of HVJ-liposome-DNA-sensitized endothelial cells
incubated in the presence of the rabbit anti-human HGF antibody.
The cell growth rate (%) is expressed in terms of relative % when
the growth rate in the control group is made 100 (*: P<0.01 for
the control group, # P<0.05 for HGF). As shown in FIG. 3, the
growth of HVJ-liposome-DNA-sensitized endothelial cells was
arrested in the presence of the anti-human HGF antibody, and the
cell growth rate was thus substantially the same as that of the
control group. These results clearly demonstrate that HGF is the
growth factor of endothelial cells.
Test Example 3
[0086] Effects of the Supernatant of the Incubated
HVJ-Liposome-DNA-Sensit- ized Rat VSMCs on Rat Coronary Endothelial
Cells
[0087] The supernatant from the incubated
HVJ-liposome-DNA-sensitized rat VSMCs were added to the rat
coronary endothelial cell culture system (cell count: 10.sup.5)
during the stationary phase. After incubation was conducted for 3
days, the count of the endothelial cells increased was examined.
For control, the supernatant of the incubated
HVJ-liposome-cont-sensitized rat VSMCs were treated in the similar
manner as described above, and the endothelial cells increased were
counted as described above. The results are shown in FIG. 4 (n=6),
wherein "control" indicates the group added with the supernatant
from the incubated HVJ-liposome-cont-sensitized rat VSMCs, and
"HGF" represents the group added with the supernatant of the
incubated HVJ-liposome-DNA-sensitized rat VSMCs.
[0088] As shown in FIG. 4, a significant increase in the count of
endothelial cells was noted in the group added with the supernatant
of the incubated HVJ-liposome-DNA-sensitized rat VSMCs.
[0089] The concentration of HGF in the culture supernatant of the
rat VSMCs sensitized with HVJ-liposome-DNA or HVJ-liposome-cont as
described above was assayed by ELISA using an anti-human HGF
antibody and an anti-rat HGF antibody. The HGF concentration in the
culture supernatant of non-sensitized VSMCs was also assayed
(no-treatment group).
[0090] The results obtained using the anti-human HGF antibody and
the anti-rat HGF antibody are shown in FIGS. 5 and 6, respectively
(n=6 in both tests). In the figure, "control" designates the group
of the supernatant from the incubated HVJ-liposome-cont-sensitized
rat VSMCs; and "HGF" designates the group of the supernatant from
the incubated HVJ-liposome-DNA-sensitized rat VSMCs.
[0091] As shown in FIG. 5, HGF was detected in the supernatant of
the HVJ-liposome-DNA-sensitized rat VSMCs, and the HGF
concentration was significantly higher than that of the control
group.
[0092] FIG. 6 also reveals that rat HGF was further detected in the
supernatant of the HVJ-liposome-DNA-sensitized rat VSMCs, and the
HGF concentration was significantly higher than that of the control
group.
[0093] As observed in FIGS. 5 and 6, no HGF was present in an
amount detectable by ELISA, in both the supernatants of the intact
group and the control group.
Test Example 4
[0094] Effects of the Supernatant from the Incubated
HVJ-Liposome-DNA-Sensitized Rat Coronary Endothelial Cells on Rat
Coronary Endothelial Cells
[0095] The supernatant of the incubated HVJ-liposomeDNA-sensitized
rat coronary endothelial cells were added to the rat coronary
endothelial cell culture system (cell count: 10.sup.5) during the
stationary phase. After incubation for 3 days, the count of the
increased endothelial cells was examined. For control, the
endothelial cells were incubated in the similar manner, using the
culture supernatant of the HVJ-liposome-cont-sensitized rat
coronary endothelial cells, and the increased endothelial cells
were counted. The results are shown in FIG. 7, wherein A, B and C
represents, respectively, the group added with the culture
supernatant of the HVJ-liposome-DNA-sensitized rat coronary
endothelial cells (n=8), the group added with the culture
supernatant of the HVJ-liposome-cont-sensitized rat coronary
endothelial cells (n=8), and the no-treatment group (n=15).
[0096] As shown in FIG. 7, a significant increase in the count of
endothelial cells was noted in the group added with the culture
supernatant of the HVJ-liposomeDNA-sensitized rat coronary
endothelial cells, whereas in the control group, the cell count was
almost the same as that of the no-treatment group (control group:
0.117.+-.0.002, group A: 0.148.+-.0.03, P<0.01).
[0097] Next, an anti-HGF antibody was added to the culture
supernatant of the HVJ-liposome-DNA-sensitized rat coronary
endothelial cells. The count of increased endothelial cells was
examined as described above. The results are shown in FIG. 8 (n=8),
wherein A represents the group added with the culture supernatant
of the HVJ-liposome-DNA-sensitized rat coronary endothelial cells;
B represents the group added with the culture supernatant of the
HVJ-liposome-cont-sensitized rat coronary endothelial cells; C
represents the group added with the anti-HGF antibody to the
culture supernatant of the HVJ-liposome-DNA-sensitized rat coronary
endothelial cells; and D represents the group added with a control
antibody to the supernatant from the incubated
HVJ-liposome-DNA-sensitize- d rat coronary endothelial cells.
[0098] As shown in FIG. 8, A and C, the cell growth promoting
activity of the culture supernatant of the
HVJ-liposome-DNA-sensitized rat coronary endothelial cells
completely disappeared by adding the anti-HGF antibody thereto. The
results reveal that the cell growth promoting activity of the
culture supernatant of the HVJ-liposome-DNA-sensitized rat coronary
endothelial cells is attributed to HGF.
Test Example 5
[0099] Effects of HVJ-Liposome-DNA-Sensitized Human VSMCs on Human
Endothelial Cells
[0100] Human VSMCs were inoculated on a cell culture insert
(manufactured by Coaster, pore diameter of 0.45 .mu.m), which were
then grown in DMEM medium supplemented with 10% bovine serum. On
the other hand, human endothelial cells were inoculated on a 6-well
plate and maintained in DMEM medium supplemented with 10% bovine
serum. When VSMCs were proliferated to reach 80% confluence, VSMCs
were incubated at 4.degree. C. for 5 minutes and at 37.degree. C.
for 30 minutes together with HVJ-liposome-DNA (DNA content in the
liposomes: 10 .mu.g) or with HVJ-liposome-cont. After
sensitization, the insert containing the sensitized VSMCs was added
to each well containing human endothelial cells in the stationary
phase. VSMCs and the endothelial cells were co-incubated for 3 days
in DMEM medium supplemented with 0.5% bovine serum. Thereafter the
cell count was determined with a WST-cell counter kit (manufactured
by Wako Co.). The results are shown in FIG. 9 (n=6). In the figure,
"control" represents the group co-incubated with the
HVJ-liposome-cont-sensitized VSMCs, and "HGF" represents the group
of the supernatant from the incubated HVJ-liposome-DNA-sensitized
VSMCs.
[0101] The results shown in FIG. 9 reveal that human VSMCs
sensitized with HVJ-liposome-DNA could significantly increase the
growth of non-sensitized human endothelial cells in the stationary
phase.
Test Example 6
[0102] Effects of the HVJ-Liposome-DNA-Sensitized Rat VSMCs on Rat
Coronary Endothelial Cells
[0103] The HVJ-liposome-DNA-sensitized rat VSMCs (cell count: 106)
were co-incubated for 3 days with rat coronary endothelial cells
(cell count: 105%) in the stationary phase. Thereafter, the count
of the increased endothelial cells was examined. For control,
endothelial cells were co-incubated in the similar manner using the
HVJ-liposome-cont-sensitized rat VSMCs, and the increased
endothelial cells were counted. The results are shown in FIG. 10
(n=6), wherein "control" represents the rat VSMCs group sensitized
with the HVJ-liposome-DNA, and "HGF" represents the rat VSMCs group
sensitized with HVJ-liposome-cont.
[0104] As shown in FIG. 10, the growth of the endothelial cells was
stimulated by HGF released from the HVJ-liposome-DNA-sensitized rat
VSMCs, and the increased cell count was observed (control group:
0.126.+-.0.006, HGF group: 0.156.+-.0.01, P<0.05).
Test Example 7
[0105] Growth of Rat VSMCs Sensitized with HVJ-Liposome-DNA
[0106] Rat VSMCs sensitized with HVJ-liposome-DNA and r at VSMCs
sensitized with HVJ-liposome-cont were incubated, respectively, to
make comparison of the increased cell count therebetween.
Sensitization with HVJ-liposome-DNA did not affect cell growth at
all. The results reveal that HGF has no cell growth promoting
effect on VSMCs.
Test Example 8
[0107] Induction of Angiogenesis in Rat Heart Muscle Directly
Injected with HVJ-Liposome-DNA
[0108] Rat heart muscle directly injected with HVJ-liposome-DNA,
rat heart muscle directly injected with HVJ-liposome-cont and rat
no-treatment heart muscle were stained with HE and Azan,
respectively, and examined on a microscope to count the number of
microvessels. The results are shown in FIG. 11, wherein "HGF"
designates the number of microvessels in rat heart muscle directly
injected with HVJ-liposome-DNA, and "control" designates the number
of microvessels in rat heart muscle directly injected with
HVJ-liposome-cont.
[0109] As is noted from FIG. 11, the number of minute blood vessels
significantly increased in the rat heart muscle injected with
HVJ-liposome-DNA, as compared to those of the rat heart muscle
injected with HVJ-liposome-cont and the rat no-treatment heart
muscle. These results reveal that HGF having the activity of
growing endothelial cells exhibits an angiogenesis activity in
vivo.
Test Example 9
[0110] Repair of Articular Cartilage by Directly Introducing
HVJ-Liposome-DNA into the Joint
[0111] Ten weeks aged Fischer's rats were injured at the femoral
intercondylaris through subcartilage using Kirschner's wires of 1.8
mm in diameter. One week after the operation, the HVJ-liposome-DNA
(100 .mu.l/knee) prepared in Example 1 was introduced directly into
the joint. For control, the HVJ-liposome-cont prepared in
Comparative Example 1 and HVJ-liposome-DNA (TGF-.beta.) prepared in
Comparative Example 2 were administered directly into the joint in
the same amount. Rats were sacrificed 1, 3 and 4 weeks after
introduction of these genes, etc. and the repaired sites were
histologically observed.
[0112] As shown in FIG. 12, the results indicate that the synthesis
of proteoglycan stained with Toluidine Blue was observed 3 weeks
after administration of the HVJ-liposome-DNA into the joint,
displaying the development of cartilage-like cells. Furthermore, as
shown in FIG. 13, 4 weeks after administration of the
HVJ-liposome-DNA into the joint, there was observed a tendency to
further extend the area of developing cartilage-like cells, in
which the synthesis of proteoglycan was recognized.
[0113] As shown in FIG. 14, where the HVJ-liposome-DNA (TGF-.beta.)
prepared in Comparative Example 2 was injected into the joint,
development of such cartilage-like cells was not observed even 4
weeks after the administration. Furthermore, as shown in FIG. 15,
where the HVJ-liposome-cont prepared in Comparative Example 1 was
injected into the joint, development of such cartilage-like cells
was not observed even 4 weeks after the administration.
INDUSTRIAL APPLICABILITY
[0114] The medicament of the present invention provides persistent
therapeutic effects, as compared to HGF itself. Moreover, the
medicament of the present invention may be topically applied to the
target organs so that the effects can be selectively exhibited,
resulting in minimizing the side effects of HGF.
* * * * *