U.S. patent application number 10/468372 was filed with the patent office on 2004-04-29 for ocular tear growth factor-like protein.
Invention is credited to Laurie, Gordon W., Lumsden, Angela J., Rajesh, Kumar, Sanghi, Sandhya.
Application Number | 20040081984 10/468372 |
Document ID | / |
Family ID | 23029096 |
Filed Date | 2004-04-29 |
United States Patent
Application |
20040081984 |
Kind Code |
A1 |
Laurie, Gordon W. ; et
al. |
April 29, 2004 |
Ocular tear growth factor-like protein
Abstract
The present invention relates to a novel lacrimal gland protein
(designated lacritin) and the nucleic acid sequences encoding that
protein. Lacritin has activity as a growth factor on both human
corneal epithelial cells and on the lacrimal acinar cells that
produce it Accordingly, one embodiment of the present invention is
directed to the use of lacritin to treat Dry Eye and other
disorders requiring the wetting of the eye.
Inventors: |
Laurie, Gordon W.;
(Charlottesville, VA) ; Sanghi, Sandhya; (Temple,
TX) ; Rajesh, Kumar; (Temple, TX) ; Lumsden,
Angela J.; (Mosman Park, AU) |
Correspondence
Address: |
UNIVERSITY OF VIRGINIA PATENT FOUNDATION
1224 WEST MAIN STREET, SUITE 1-110
CHARLOTTESVILLE
VA
22903
US
|
Family ID: |
23029096 |
Appl. No.: |
10/468372 |
Filed: |
August 19, 2003 |
PCT Filed: |
February 20, 2002 |
PCT NO: |
PCT/US02/04971 |
Current U.S.
Class: |
435/6.15 ;
435/320.1; 435/325; 435/69.1; 514/20.8; 514/7.6; 530/399;
536/23.5 |
Current CPC
Class: |
A61P 27/02 20180101;
C07K 14/475 20130101; A61K 9/0048 20130101; A61P 27/04 20180101;
A61P 43/00 20180101 |
Class at
Publication: |
435/006 ;
435/069.1; 435/320.1; 435/325; 530/399; 514/012; 536/023.5 |
International
Class: |
C12Q 001/68; C07K
014/475; A61K 038/18; C07H 021/04 |
Goverment Interests
[0002] This invention was made with United States Government
support under Grant No. R01 EY09747 and R01 EY13143, awarded by
National Institutes of Health. The United States Government has
certain rights in the invention.
Foreign Application Data
Date |
Code |
Application Number |
Feb 20, 2001 |
US |
60269900 |
Claims
1. A purified polypeptide comprising the amino acid sequence of SEQ
ID NO: 4, or an antigenic fragment of SEQ ID NO: 4.
2. A composition comprising amino acid sequence of SEQ ID NO: 4 and
a pharmaceutically acceptable carrier.
3. The composition of claim 2, wherein the composition is a topical
ophthalmic formulation.
4. The composition of claim 3, further comprising a
pharmaceutically acceptable phospholipid or oil.
5. A purified nucleic acid sequence that hybridizes to a 100
nucleotide fragment of SEQ ID NO: 1 or its complement under
stringent conditions.
6. A purified nucleic acid sequence comprising a 25 bp nucleic acid
sequence that is identical to a contiguous 25 bp sequence of SEQ ID
NO: 2 or SEQ ID No: 5.
7. The nucleic acid sequence of claim 6 comprising a sequence
selected from the group consisting of SEQ ID NO: 3, SEQ ID NO 5 and
SEQ ID NO: 6.
8. A nucleic acid construct comprising a nucleic acid sequence
selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6,
SEQ ID NO: 7 and SEQ ID NO: 8 operably linked to a heterologous
gene.
9. The nucleic acid construct of claim 8 wherein said heterologous
gene encodes a marker.
10. The nucleic acid construct of claim 9, wherein the marker
produces a fluorescent signal.
11. The nucleic acid construct of claim 9 wherein said construct is
a plasmid.
12. A transgenic host cell comprising the nucleotide sequence of
claim 8, 9 or 11.
13. A nucleic acid construct comprising a nucleic acid sequence
selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6,
SEQ ID NO: 7 and SEQ ID NO: 8 operably linked to a polylinker.
14. The nucleic acid construct of claim 13 wherein said construct
is a plasmid.
15. A purified polypeptide comprising the amino acid sequence of
SEQ ID NO: 4, or an amino acid sequence that differs from SEQ ID
NO: 4 by one or more conservative amino acid substitutions; or an
amino acid sequence that differs from SEQ ID NO: 4 by a single
mutation, wherein the single mutation represents a single amino
acid deletion, insertion or substitution.
16. An antibody that binds specifically to the protein of SEQ ID
NO: 4.
17. A method for identifying a test compound capable of modulating
lacritin activity, said method comprising: (a) contacting a
solution containing lacritin with said test compound; and (b)
measuring lacritin activity in the presence and absence of said
test compound, such that if the level obtained in the presence of
the test compound differs from that obtained in its absence, then a
compound which modulates lacritin gene expression is
identified.
18. The method of claim 17 wherein the lacritin activity measured
is lacritin's ability to promote ductal cell proliferation.
19. The method of claim 17 wherein the lacritin activity measured
is lacritin's ability to promote tyrosine phosphorylation or
calcium signaling.
20. A method of treating Dry Eye, said method comprising the step
of contacting the ocular surface with a composition comprising a
polypeptide, wherein the polypeptide comprises the amino acid
sequence of SEQ ID NO: 4, or an amino acid sequence that differs
from SEQ ID NO: 4 by one or more conservative amino acid
substitutions; or an amino acid sequence that differs from SEQ ID
NO: 4 by a single mutation, wherein the single mutation represents
a single amino acid deletion, insertion or substitution.
21. A method of enhancing the proliferation of human corneal
epithelial cells or lacrimal acinar cells, said method comprising
the step of contacting said cells with a composition comprising a
polypeptide, wherein the polypeptide comprises the amino acid
sequence of SEQ ID NO: 4, or an amino acid sequence that differs
from SEQ ID NO: 4 by one or more conservative amino acid
substitutions; or an amino acid sequence that differs from SEQ ID
NO: 4 by a single mutation, wherein the single mutation represents
a single amino acid deletion, insertion or substitution.
22. A method for detecting a lacritin receptor, said method
comprising providing a cell that has been transfected with nucleic
acid sequences that encode for potential cell receptors; contacting
said transfected cells with lacritin; detecting cells that display
lacritin-dependent calcium signaling; isolating nucleic acid
sequences from the detected cells; and identifying a nucleic acid
sequence that confers lacritin-dependent calcium signaling on cells
transfected with that nucleic acid sequence.
Description
RELATED APPLICATION
[0001] This application claims priority under 35 USC .sctn.199(e)
to U.S. Provisional Application Serial No. 60/269,900, filed Feb.
20, 2001, the disclosure of which is incorporated herein.
FIELD OF THE INVENTION
[0003] The present invention is directed to a novel ocular protein,
designated lacritin, and nucleic acid sequences encoding that
protein. In one embodiment of the invention compositions comprising
lacritin are used to enhance corneal wound healing, and/or treat
patients having deficient tear output.
BACKGROUND OF THE INVENTION
[0004] Health of the ocular surface is dependent on tear fluid
secretions from the lacrimal gland. The lacrimal acinar cells
comprising the lacrimal gland are polarized and highly
differentiated tear secreting cells that adhere to a complex
periacinar basement membrane. The bulk of the apical cell cytoplasm
contains large secretory granules packed with tear proteins. Known
tear proteins include: lysozyme, which plays a prominent
bacteriocidal role on the corneal surface; lactoferrin, which
functions as both a bacteriocidal agent and as a potential
inhibitor of complement activation; secretory component, which
regulates the transcellular movement of IgA into acini lumen where
it acts on the comeal surface to inhibit bacterial adhesion; and
tear lipocalins (tear-specific prealbumin) and growth factors
TGF.alpha., TGF.beta.and EGF the functions of which are not known.
In rats, peroxidase is a tear component which has served as a
convenient marker in experimental studies. Tears not only have an
important bacteriocidal role, they also keep the cornea clean and
lubricated and are important for the well-being of the corneal
epithelium.
[0005] When lacrimal acinar cell tear output is collectively
deficient, `Dry Eye` (also known as keratoconjunctivitis sicca
[KCS]); is the result. Dry Eye is a common ocular manifestation of
Sjogren's syndrome, an autoimmune disease with unknown etiology
that affects millions of people worldwide. Most commonly affected
are post-menopausal women with varying degrees of severity. If
untreated, Dry Eye can lead to corneal abrasion, ulceration,
bacterial infection and loss of vision. Molecular mechanisms
underlying the pathogenic decline of secretory output by the main
lacrimal gland are potentially multiple. Lacrimal glands of
Sjogren's syndrome patients contain foci of B and T lymphocytes
whose pathogenic expansion, possibly due to viral insult, can
destroy lacrimal acini. However, acinar volume loss often appears
insufficient relative to the theoretical overcapacity of the main
lacrimal gland. Estimates suggest a potential secretory output up
to ten-fold greater than is required to maintain a normal aqueous
tear film layer. Other mechanisms therefore warrant attention, such
as aberrant secretion of one or several common cytokines that may
directly or indirectly alter lacrimal acinar cell function and/or
lead to a decline in neural innervation. Novel autocrine/paracrine
factor(s) released by lacrimal acinar cells into the tear film may
be required for the health of the lacrimal secretory. machinery,
ductal system and corneal epithelium. The periacinar basement
membrane is also required for normal secretory function, in part
via `BM180`whose apparent synergy with laminin-l promotes
stimulated tear secretion. Alteration of each of these factors,
together or independent of hormonal changes, could contribute to
decreased secretory capacity.
[0006] Existing protocols for treating Dry Eye suffer from several
limitations. In particular, topical artificial tear replacement
fluids are widely distributed by a number of pharmaceutical
companies, but the efficacy is poor and short-lived. This lack of
efficacy is due in part to the fact that constituents of natural
human tears are only partially known.
[0007] The present invention is directed to a novel human
extracellular glycoprotein termed `lacritin `that is remarkably
reduced in Sjogren's syndrome. Furthermore lacritin has been found
to act in an autocrine manner to enhance unstimulated (but not
stimulated) tear secretion. Lacritin is produced by lacrimal acinar
cells and released for the most part into tear fluid--much like
acinar cell-expressed TGF.beta.'s. This glycoprotein acts like a
growth factor when added in purified recombinant form to cultures
of human corneal epithelial cells, and in a feedback mechanism, it
also appears to act on the same lacrimal gland cells that produce
it. Accordingly in one embodiment of the present invention,
lacritin is included as an active agent in artificial tear
products.
SUMMARY OF THE INVENTION
[0008] The present invention is directed to the isolation and
characterization of a novel lacrimal gland protein and the nucleic
acid sequences encoding that protein. Purified recombinant lacritin
has activity as a growth factor on both human corneal epithelial
cells and on the lacrimal acinar cells that produce it.
Accordingly, in one embodiment of the present invention a method is
provided for treating Dry Eye and other disorders requiring the
wetting of the eye by administering compositions comprising a
lacritin polypeptide. In addition, since the gene promoter
regulating lacritin gene expression is the most specific of any
previously described lacrimal gland gene, the regulatory elements
of this gene could be used to express other gene products in the
eye.
BRIEF DESCRIPTION OF THE DRAWINGS
[0009] FIG. 1 is a graphic representation that shows recombinant
lacritin enhances unstimulated secretion by isolated rat lacrimal
acinar cells. Enhancement of unstimulated secretion was observed in
the presence of increasing amounts of lacritin on lacritin-coated
wells.
[0010] FIG. 2A and 2B represent lacritin-induced proliferation and
tyrosine phosphorylation. FIG. 2A is a graphic representation of
the number of human salivary gland (HSG) cells was determined four
days after administering various amounts of lacritin (0 to 10 ng/ml
of lacritin) to HSG cells in serum-free medium. FIG. 2B is a bar
graph representing the proliferation of HSG cells upon
administration of BSA (lane1; 10 ng/ml) or serum (lane 2; 10%) was
added for the same period of time. All experiments were performed
on laminin-1-(0.05 .mu.M) coated wells.
DETAILED DESCRIPTION OF THE INVENTION
[0011] Definitions
[0012] In describing and claiming the invention, the following
terminology will be used in accordance with the definitions set
forth below.
[0013] As used herein, "nucleic acid," "DNA," and similar terms
also include nucleic acid analogs, i.e. analogs having other than a
phosphodiester backbone. For example, the so-called "peptide
nucleic acids," which are known in the art and have peptide bonds
instead of phosphodiester bonds in the backbone, are considered
within the scope of the present invention.
[0014] The term "peptide" encompasses a sequence of 3 or more amino
acids wherein the amino acids are naturally occurring or synthetic
(non-naturally occurring) amino acids. Peptide mimetics include
peptides having one or more of the following modifications:
[0015] 1 . peptides wherein one or more of the peptidyl --C(O)NR--
linkages (bonds) have been replaced by a non-peptidyl linkage such
as a --CH2-carbamate linkage (--CH2OC(O)NR--), a phosphonate
linkage, a --CH2-sulfonamide (--CH 2--S(O)2NR--) linkage, a urea
(--HC(O)NH--) linkage, a --CH2-secondary amine linkage, or with an
alkylated peptidyl linkage (--C(O)NR--) wherein R is C1-C4
alkyl;
[0016] 2. peptides wherein the N-terminus is derivatized to a
--NRR1 group, to a --NRC(O)R group, to a --NRC(O)OR group, to a
--NRS(O)2R group, to a --NHC(O)NHR group where R and R1 are
hydrogen or C1-C4 alkyl with the proviso that R and R1 are not both
hydrogen;
[0017] 3. peptides wherein the C terminus is derivatized to
--C(O)R2 where R 2 is selected from the group consisting of C1-C4
alkoxy, and --NR3R4 where R3 and R4 are independently selected from
the group consisting of hydrogen and C1-C4 alkyl.
[0018] Naturally occurring amino acid residues in peptides are
abbreviated as recommended by the IUPAC-IUB Biochemical
Nomenclature Commission as follows: Phenylalanine is Phe or F;
Leucine is Leu or L; Isoleucine is Ile or I; Methionine is Met or
M; Norleucine is Nle; Valine is Val or V; Serine is Ser or S;
Proline is Pro or P; Threonine is Thr or T; Alanine is Ala or A;
Tyrosine is Tyr or Y; Histidine is His or H; Glutamine is Gln or Q;
Asparagine is Asn or N; Lysine is Lys or K; Aspartic Acid is Asp or
D; Glutamic Acid is Glu or E; Cysteine is Cys or C; Tryptophan is
Trp or W; Arginine is Arg or R; Glycine is Gly or G, and X is any
amino acid. Other naturally occurring amino acids include, by way
of example, 4-hydroxyproline, 5-hydroxylysine, and the like.
[0019] Synthetic or non-naturally occurring amino acids refer to
amino acids which do not naturally occur in vivo but which,
nevertheless, can be incorporated into the peptide structures
described herein. The resulting "synthetic peptide" contain amino
acids other than the 20 naturally occurring, genetically encoded
amino acids at one, two, or more positions of the peptides. For
instance, naphthylalanine can be substituted for trytophan to
facilitate synthesis. Other synthetic amino acids that can be
substituted into peptides include L-hydroxypropyl,
L-3,4-dihydroxyphenylalanyl, alpha-amino acids such as
L-alpha-hydroxylysyl and D-alpha-methylalanyl,
L-alpha.-methylalanyl, beta.-amino acids, and isoquinolyl. D amino
acids and non-naturally occurring synthetic amino acids can also be
incorporated into the peptides. Other derivatives include
replacement of the naturally occurring side chains of the 20
genetically encoded amino acids (or any L or D amino acid) with
other side chains.
[0020] As used herein, the term "conservative amino acid
substitution" are defined herein as exchanges within one of the
following five groups:
[0021] I. Small aliphatic, nonpolar or slightly polar residues:
[0022] Ala, Ser, Thr, Pro, Gly;
[0023] II. Polar, negatively charged residues and their amides:
[0024] Asp, Asn, Glu, Gln;
[0025] III. Polar, positively charged residues:
[0026] His, Arg, Lys;
[0027] IV. Large, aliphatic, nonpolar residues:
[0028] Met Leu, Ile, Val, Cys
[0029] V. Large, aromatic residues:
[0030] Phe, Tyr, Trp
[0031] A "polylinker" is a nucleic acid sequence that comprises a
series of three or more different restriction endonuclease
recognitions sequences closely spaced to one another (i.e. less
than 10 nucleotides between each site).
[0032] As used herein, the term "vector" is used in reference to
nucleic acid molecules that has the capability of replicating
autonomously in a host cell, and optionally may be capable of
transferring DNA segment(s) from one cell to another. Vectors can
be used to introduce foreign DNA into host cells where it can be
replicated (i.e., reproduced) in large quantities. Examples of
vectors include plasmids, cosmids, lambda phage vectors, viral
vectors (such as retroviral vectors).
[0033] As used herein a "gene" refers to the nucleic acid coding
sequence as well as the regulatory elements necessary for the DNA
sequence to be transcribed into messenger RNA (mRNA) and then
translated into a sequence of amino acids characteristic of a
specific polypeptide.
[0034] A "marker" is an atom or molecule that permits the specific
detection of a molecule comprising that marker in the presence of
similar molecules without such a marker. Markers include, for
example radioactive isotopes, antigenic determinants, nucleic acids
available for hybridization, chromophors, fluorophors,
chemiluminescent molecules, electrochemically detectable molecules,
molecules that provide for altered fluorescence-polarization or
altered light-scattering and molecules that allow for enhanced
survival of an cell or organism (i.e. a selectable marker). A
reporter gene is a gene that encodes for a marker.
[0035] A promoter is a DNA sequence that directs the transcription
of a DNA sequence, such as the nucleic acid coding sequence of a
gene. Typically, a promoter is located in the 5' region of a gene,
proximal to the transcriptional start site of a structural gene.
Promoters can be inducible (the rate of transcription changes in
response to a specific agent), tissue specific (expressed only in
some tissues), temporal specific (expressed only at certain times)
or constitutive (expressed in all tissues and at a constant rate of
transcription).
[0036] A core promoter contains essential nucleotide sequences for
promoter function, including the TATA box and start of
transcription. By this definition, a core promoter may or may not
have detectable activity in the absence of specific sequences that
enhance the activity or confer tissue specific activity.
[0037] An "enhancer" is a DNA regulatory element that can increase
the efficiency of transcription, regardless of the distance or
orientation of the enhancer relative to the start site of
transcription.
[0038] As used herein, the terms "complementary" or
"complementarity" are used in reference to polynucleotides (i.e., a
sequence of nucleotides) related by the base-pairing rules. For
example, for the sequence "A-G-T," is complementary to the sequence
"T-C-A."
[0039] As used herein, the term "hybridization" is used in
reference to the pairing of complementary nucleic acids.
Hybridization and the strength of hybridization (i.e., the strength
of the association between the nucleic acids) is impacted by such
factors as the degree of complementarity between the nucleic acids,
stringency of the conditions involved, the length of the formed
hybrid, and the G:C ratio within the nucleic acids.
[0040] As used herein, the term "purified" and like terms relate to
the isolation of a molecule or compound in a form that is
substantially free of contaminants normally associated with the
molecule or compound in a native or natural environment.
[0041] As used herein, the term "lacritin polypeptide" and like
terms refers to peptides comprising the amino acid sequence of SEQ
ID NO: 4 and biologically active fragments thereof.
[0042] As used herein, the term "biologically active fragments" or
"bioactive fragment" of an lacritin polypeptide encompasses natural
or synthetic portions of the amino acid sequence
MKFTTLLFLAAVAGALVYAEDASSDST- GADPAQEAGTSKPNEEI
SGPAEPASPPETTTTAQETSAAAVQGTAKVTSSRQELNPLKS IVEKSILLTEQALAKAGKGM
HGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA (SEQ ID NO: 4) that are capable of
specific binding to at least one of the natural ligands of the
native lacritin polypeptide.
[0043] "Operably linked" refers to a juxtaposition wherein the
components are configured so as to perform their usual function.
Thus, control sequences or promoters operably linked to a coding
sequence are capable of effecting the expression of the coding
sequence.
[0044] As used herein, the term "pharmaceutically acceptable
carrier" encompasses any of the standard pharmaceutical carriers,
such as a phosphate buffered saline solution, water and emulsions
such as an oil/water or water/oil emulsion, and various types of
wetting agents.
[0045] As used herein, the term "treating" includes alleviating the
symptoms associated with a specific disorder or condition and/or
preventing or eliminating said symptoms.
[0046] The Invention
[0047] The present invention is directed to a novel human growth
factor-like molecule, `lacritin `and compositions comprising
lacritin. The invention also encompasses the nucleic acid sequences
encoding lacritin as well as the nucleic acid regulatory elements
controlling the expression of lacritin. The full length `lacritin
`cDNA has been cloned from a human lacrimal gland library (SEQ ID
NO:2), and the corresponding genomic gene (SEQ ID NO: 1) has been
cloned and sequenced, including 5.2 kb of upstream and 2.8 kb of
downstream genomic sequence.
[0048] In one embodiment, the present invention is directed to a
purified polypeptide comprising the amino acid sequence of SEQ ID
NO: 4, SEQ ID NO: 10, a bioactive fragment of SEQ ID NO: 4, or an
amino acid sequence that differs from SEQ ID NO: 4 by one or more
conservative amino acid substitutions. More preferably, the
purified polypeptide comprises an amino acid sequence that differs
from SEQ ID NO: 4 by 20 or less conservative amino acid
substitutions, and more preferably by 10 or less conservative amino
acid substitutions. Alternatively, the polypeptide may comprise an
amino acid sequence that differs from SEQ ID NO: 4 by 1 to 5
alterations, wherein the alterations are independently selected
from a single amino acid deletion, insertion or substitution. In
one preferred embodiment a composition is provided comprising a
polypeptide, selected from the group consisting of SEQ ID NO: 4, or
SEQ ID NO: 10, and a pharmaceutically acceptable carrier.
[0049] Also encompassed in the present invention are ligands that
bind to the lacritin polypeptide, including the natural receptor
for lacritin, as well as methods for isolating such ligands. In one
embodiment the lacritin polypeptide, or bioactive fragments
thereof, is used to isolate ligands that bind to the lacritin
polypeptide under physiological conditions. The method comprises
the steps of contacting the lacritin polypeptide with a mixture of
compounds under physiological conditions, removing unbound and
non-specifically bound material, and isolating the compounds that
remain bound to the lacritin polypeptides. Typically, the lacritin
polypeptide will be bound to a solid support using standard
techniques to allow for rapid screening of compounds. The solid
support can be selected from any surface that has been used to
immobilize biological compounds and includes but is not limited to
polystyrene, agarose, silica or nitrocellulose. In one embodiment
the solid surface comprises functionalized silica or agarose beads.
Screening for such compounds can be accomplished using libraries of
pharmaceutical agents and standard techniques known to the skilled
practitioner.
[0050] In an alternative embodiment a cell based assay is used to
detect ligands that bind to lacritin (including lacritin's natural
receptor). The method comprises contacting transfected cells with
lacritin and isolating the relevant genes from those cells that
display lacritin-dependent calcium signaling. More particularly, in
one embodiment, previously described pools of orphan G protein
coupled receptor cDNA's will be expressed in cell lines such as
HEK293T and RH7777 cells, and the transfected cells will be
contacted with lacritin. A transfectant that displays
lacritin-dependent calcium signaling should be expressing the
receptor. If the receptor is not detected in the available pool of
orphan G protein coupled receptor cDNA's, cDNA's from a salivary
ductal cell library will be transfected into 293T cells, and
expressors screened by FACS with fluorescently labeled lacritin. In
accordance with one embodiment cells expressing receptors that can
be activated by lacritin will be detected using a cell free system.
More particularly, receptor activity will be detected via a
GTP[.gamma.35 S] binding assay using isolated cell membranes from
the transfected cells.
[0051] In one aspect of the invention a method for detecting the
lacritin receptor is provided. The method comprises the steps of
providing a cell that has been transfected with nucleic acid
sequences that encode for potential cell receptors, contacting the
transfected cells with lacritin and detecting those cells that
display lacritin-dependent calcium signaling. If the cells
displaying lacritin-dependent calcium signaling were transfected
with more than one protein encoding gene sequence, than the nucleic
acid sequences encoding for the lacritin receptor will be
identified by sequence analysis or other molecular technique. For
example, the introduced recombinant nucleic acids will be isolated
from the signaling cells and further subcloned with the resulting
subclones used to transfect cells to determine the unique sequence
responsible for conferring lacritin-dependent calcium signaling to
a cell.
[0052] The present invention also encompasses nucleic acid
sequences that encode the lacritin polypeptide and derivatives
thereof. In particular the present invention is directed to nucleic
acid sequences comprising the sequence of SEQ ID NO: 1, SEQ ID NO:
2, SEQ ID NO: 3, or fragments thereof. In one embodiment, purified
nucleic acids comprising at least 8 contiguous nucleotides (i. e.,
a hybridizable portion) that are identical to any 8 contiguous
nucleotides of SEQ ID NO: 1 are provided. In other embodiments, the
nucleic acids comprises at least 25 (contiguous) nucleotides, 50
nucleotides, 100 nucleotides, 200 nucleotides, or 500 nucleotides
of SEQ ID NO: 1. In another embodiment the nucleic acid sequence
comprises the sequence of SEQ ID NO: 3 or a 25 bp nucleic acid
sequence that is identical to a contiguous 25 bp sequence of SEQ ID
NO: 3.
[0053] The present invention also includes nucleic acids that
hybridize (under conditions defined herein) to all or a portion of
the nucleotide sequence represented by SEQ ID NO: 1 or its
complement. The hybridizing portion of the hybridizing nucleic
acids is typically at least 15 (e.g., 20, 25, 30, or 50)
nucleotides in length. Hybridizing nucleic acids of the type
described herein can be used, for example, as a cloning probe, a
primer (e.g., a PCR primer), or a diagnostic probe. It is
anticipated that the DNA sequence of SEQ ID NO: 1, or fragments
thereof can be used as probes to detect homologous genes from other
vertebrate species.
[0054] Nucleic acid duplex or hybrid stability is expressed as the
melting temperature or Tm, which is the temperature at which a
nucleic acid duplex dissociates into its component single stranded
DNAs. This melting temperature is used to define the required
stringency conditions. Typically a 1% mismatch results in a 1
.degree. C. decrease in the Tm, and the temperature of the final
wash in the hybridization reaction is reduced accordingly (for
example, if two sequences having >95% identity, the final wash
temperature is decreased from the Tm by 5.degree. C.). In practice,
the change in Tm can be between 0.5.degree. C. and 1.5.degree. C.
per 1% mismatch.
[0055] The present invention is directed to the nucleic acid
sequence of SEQ ID NO: 1 and nucleic acid sequences that hybridize
to that sequence (or fragments thereof) under stringent or highly
stringent conditions. In one embodiment the invention is directed
to a purified nucleic acid sequence that hybridizes to a 100
nucleotide fragment of SEQ ID NO: 1 or its complement under
stringent conditions. In accordance with the present invention
highly stringent conditions are defined as conducting the
hybridization and wash conditions at no lower than -5.degree. C.
Tm. Stringent conditions are defined as involve hybridizing at
68.degree. C. in 5.times.SSC/5.times. Denhardt's solution/1.0% SDS,
and washing in 0.2.times. SSC/0.1% SDS at 68.degree. C. Moderately
stringent conditions include hybridizing at 68.degree. C. in
5.times. SSC/5.times. Denhardt's solution/1.0% SDS and washing in
3.times. SSC/0.1% SDS at 42.degree. C. Additional guidance
regarding such conditions is readily available in the art, for
example, by Sambrook et al., 1989, Molecular Cloning, A Laboratory
Manual, Cold Spring Harbor Press, N.Y.; and Ausubel et al. (eds.),
1995, Current Protocols in Molecular Biology, (John Wiley &
Sons, N.Y.) at Unit 2.10.
[0056] In another embodiment of the present invention, nucleic acid
sequences encoding the lacritin polypeptide can be inserted into
expression vectors and used to transfect cells to express
recombinant lacritin in the target cells. In accordance with one
embodiment, the nucleic acid sequence of SEQ ID NO: 3 are inserted
into a eukaryotic expression vector in a manner that operably links
the gene sequences to the appropriate regulatory sequences, and
lacritin is expressed in a eukaryotic host cell. Suitable
eukaryotic host cells and vectors are known to those skilled in the
art. In particular, nucleic acid sequences encoding lacritin may be
added to a cell or cells in vitro or in vivo using delivery
mechanisms such as liposomes, viral based vectors, or
microinjection. Accordingly, one aspect of the present invention is
directed to transgenic cell lines that contain recombinant genes
that express the lacritin polypeptide of SEQ ID NO: 4.
[0057] The present invention is also directed to nucleic acid
constructs for expressing heterologous genes under the control of
the lacritin gene promoter. In accordance with one embodiment a
nucleic acid construct is provided comprising a nucleic acid
sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID
NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8 operably linked to a
heterologous gene. In accordance with one embodiment the
heterologous gene is a reporter gene that encodes for a marker. The
marker can be any gene product that produces a detectable signal
and includes proteins capable of emitting light such as Green
Fluorescent Protein (GFP) (Chalfie et al., 1994, Science 11:
263:802-805) or luciferase (Gould et al., 1988, Anal. Biochem. 15:
175: 5-13), as well as proteins that can catalyze a substrate
(e.g., such as .beta.-galactosidase). The marker may also comprise
intracellular or cell surface proteins that are detectable by
antibodies. Reporter molecules additionally, or alternatively, can
be detected by virtue of a unique nucleic acid sequence not
normally contained within the cell.
[0058] As used herein, "GFP" refers to a member of a family of
naturally occurring fluorescent proteins, whose fluorescence is
primarily in the green region of the spectrum. The term includes
mutant forms of the protein with altered or enhanced spectral
properties. Some of these mutant forms are described in Cormack, et
al., 1996, Gene 173: 33-38 and Ormo, 1996, Science 273:1392-1395,
the entireties of which are incorporated herein by reference. The
term also includes polypeptide analogs, fragments or derivatives of
GFP polypeptides which differ from naturally-occurring forms by the
identity or location of one or more amino acid residues, (e.g., by
deletion, substitution or insertion) and which share some or all of
the properties of the naturally occurring forms so long as they
generate detectable signals (e.g., fluorescence). Wild type GFP
absorbs maximally at 395 nm and emits at 509 nm. High levels of GFP
expression have been obtained in cells ranging from yeast to human
cells. The term also includes Blue Fluorescent Protein (BFP), the
coding sequence for which is described in Anderson, et al., 1996,
Proc. Natl. Acad. Sci. USA 93:16, 8508-8511, incorporated herein by
reference.
[0059] Another embodiment of the present invention comprises
antibodies that are generated against the lacritin polypeptide.
These antibodies can be formulated with standard carriers and
optionally labeled to prepare therapeutic or diagnostic
compositions. Antibodies to lacritin are generated using methods
that are well known in the art. Such antibodies may include, but
are not limited to, polyclonal, monoclonal, chimeric (i.e
"humanized" antibodies), single chain (recombinant), Fab fragments,
and fragments produced by a Fab expression library. These
antibodies can be used as diagnostic agents for the diagnosis of
conditions or diseases characterized by expression or
overexpression of lacritin, or in assays to monitor patients being
treated for a conditions or diseases characterized by inappropriate
lacritin expression. The antibodies useful for diagnostic purposes
may be prepared in the same manner as those described above for
therapeutics. The antibodies may be used with or without
modification, and may be labeled by joining them, either covalently
or non-covalently, with a marker. In accordance with one embodiment
an antibody is provided that specifically binds to the protein of
SEQ ID NO: 4, and more preferably the antibody is a monoclonal
antibody.
[0060] The invention also encompasses antibodies, including
anti-idiotypic antibodies, antagonists and agonists, as well as
compounds or nucleotide constructs that inhibit expression of the
lacritin gene (transcription factor inhibitors, antisense and
ribozyme molecules, or gene or regulatory sequence replacement
constructs), or promote expression of lacritin (e.g., expression
constructs wherein the lactritin coding sequences, such as SEQ ID
NO: 3 are operatively associated with expression control elements
such as promoters, promoter/enhancers, etc.).
[0061] The present invention also encompasses antigenic
compositions for raising antibodies against lacritin. In one
embodiment an antigenic composition is provided comprising the
polypeptide of SEQ ID NO: 4 or an antigenic fragment thereof.
[0062] Lacritin has mitogenic activity, enhances unstimulated but
not stimulated secretion, and promotes signaling in both lacrimal
acinar and corneal epithelial cells. Recombinant lacritin prepared
in E. coli specifically and rapidly activates both human corneal
epithelial cells and mouse & rat lacrimal acinar cells -the
latter in an autocrine manner to enhance tear synthesis. Lacritin
is active at ng/ml levels, and contaminating bacterial LPS
(endotoxin) is not detectable. The activities of purified
recombinant lacritin indicate that it acts as a growth factor on
both human corneal epithelial cells and on the lacrimal acinar
cells that produce it. Importantly, lacritin likely acts as a
growth factor only in the eye, and to a lesser extent in the
salivary gland. These organ-specific beneficial effects can be used
to dramatically increase the efficacy of currently available
topically artificial tear products.
[0063] Current tear supplements are not popular with patients, in
part because the relief obtained from such products is very brief
(less than 15 min). Examples of the tear substitution approach
include the use of buffered, isotonic saline solutions, aqueous
solutions containing water soluble polymers that render the
solutions more viscous and thus less easily shed by the eye. Tear
reconstitution is also attempted by providing one or more
components of the tear film such as phospholipids and oils.
Examples of these treatment approaches are disclosed in U.S. Pat.
No. 4,131,651 (Shah et al.), U.S. Pat. No. 4,370,325 (Packman),
U.S. Pat. No. 4,409,205 (Shively), U.S. Pat. Nos. 4,744,980 and
4,883,658 (Holly), U.S. Pat. No. 4,914,088 (Glonek), U.S. Pat. No.
5,075,104 (Gressel et al.) and U.S. Pat. No. 5,294,607 (Glonek et
al.) the disclosures of which are incorporated herein. Existing
ophthalmic formulations may also include TGF-beta, corticosteroids
or androgens. All are non-specific for the eye and have systemic
effects. In contrast, lacritin is highly restricted to the eye and
is a natural constituent of human tears and the tear film.
[0064] An ophthalmic formulation comprising lacritin (for example,
an artificial tear fluids containing lacritin) is highly desirable
due to the activity of lacritin and its localized effects. In
accordance with one embodiment of the invention, compositions
comprising lacritin are used to enhance corneal wound healing,
and/or treat patients having deficient tear output. More
particularly, lacritin is used in accordance with one embodiment to
treat Dry Eye syndromes, including Sjogren's syndrome and to
enhance corneal wound healing by topical application of
compositions comprising the lacritin polypeptide. In accordance
with one embodiment the composition comprises a pharmaceutically
acceptable carrier and a pharmaceutically effective amount of
substantially pure polypeptide comprising the amino acid sequence
of SEQ ID NO: 4 is used to treat Dry Eye syndromes.
[0065] The lacritin compositions of the present invention can be
formulated using standard ophthalmic components, and preferably the
compositions are formulated as solutions, suspensions and other
dosage forms for topical administration. Aqueous solutions are
generally preferred, based on ease of formulation, biological
compatibility (especially in view of the malady to be treated,
e.g., dry eye-type diseases and disorders), as well as a patient's
ability to easily administer such compositions by means of
instilling one to two drops of the solutions in the affected eyes.
However, the compositions may also be suspensions, viscous or
semi-viscous gels, or other types of solid or semi-solid
compositions.
[0066] The compositions of the present invention may include
surfactants, preservative agents, antioxidants, tonicity agents,
buffers, preservatives, co-solvents and viscosity building agents.
Various surfactants useful in topical ophthalmic formulations may
be employed in the present compositions. These surfactants may aid
in preventing chemical degradation of lacritin and also prevent the
lacritin from binding to the containers in which the compositions
are packaged. Examples of surfactants include, but are not limited
to: Cremophor.RTM. EL, polyoxyl 20 ceto stearyl ether, polyoxyl 40
hydrogenated castor oil, polyoxyl 23 lauryl ether and poloxamer 407
may be used in the compositions. Antioxidants may be added to
compositions of the present invention to protect the lacritin
polypeptide from oxidation during storage. Examples of such
antioxidants include, but are not limited to, vitamin E and analogs
thereof, ascorbic acid and derivatives, and butylated
hydroxyanisole (BHA).
[0067] Existing artificial tears formulations can also be used as
pharmaceutically acceptable carriers for the lacritin active agent.
Thus in one embodiment, lacritin is used to improve existing
artificial tear products for Dry Eye syndromes, as well as develop
products to aid corneal wound healing. Examples of artificial tears
compositions useful as carriers include, but are not limited to,
commercial products, such as Tears Naturale.RTM., Tears Naturale
II.RTM., Tears Naturale Free.RTM., and Bion Tears.RTM. (Alcon
Laboratories, Inc., Fort Worth, Tex.). Examples of other
phospholipid carrier formulations include those disclosed in U.S.
Pat. No. 4,804,539 (Guo et al.), U.S. Pat. No. 4,883,658 (Holly),
U.S. Pat. No. 4,914,088 (Glonek), U.S. Pat. No. 5,075,104 (Gressel
et al.), U.S. Pat. No. 5,278,151 (Korb et al.), U.S. Pat. No.
5,294,607 (Glonek et al.), U.S. Pat. No. 5,371,108 (Korb et al.),
U.S. Pat. No. 5,578,586 (Glonek et al.); the foregoing patents are
incorporated herein by reference to the extent they disclose
phospholipid compositions useful as phospholipid carriers of the
present invention.
[0068] Other compounds may also be added to the ophthalmic
compositions of the present invention to increase the viscosity of
the carrier. Examples of viscosity enhancing agents include, but
are not limited to: polysaccharides, such as hyaluronic acid and
its salts, chondroitin sulfate and its salts, dextrans, various
polymers of the cellulose family; vinyl polymers; and acrylic acid
polymers. In general, the phospholipid carrier or artificial tears
carrier compositions will exhibit a viscosity of 1 to 400
centipoises ("cps"). Preferred compositions containing artificial
tears or phospholipid carriers and will exhibit a viscosity of
about 25 cps.
[0069] Topical ophthalmic products are typically packaged in
multidose form. Preservatives are thus required to prevent
microbial contamination during use. Suitable preservatives include:
benzalkonium chloride, chlorobutanol, benzododecinium bromide,
methyl paraben, propyl paraben, phenylethyl alcohol, edetate
disodium, sorbic acid, polyquaternium-1, or other agents known to
those skilled in the art. Such preservatives are typically employed
at a level of from 0.001 to 1.0% w/v. Unit dose compositions of the
present invention will be sterile, but typically unpreserved. Such
compositions, therefore, generally will not contain
preservatives.
[0070] In humans, lacritin is produced by the lacrimal gland (large
amounts), salivary gland (moderate), the basal cells of the corneal
epithelium (based on inmmunostaining of human cornea by
anti-lacritin antibodies; and ELISA detection of lacritin in human
corneal epithelial cell cultures) and possibly in the thyroid, but
not elsewhere. Lacritin enhances unstimulated but not stimulated
secretion, has mitogenic activity and promotes signaling in both
lacrimal acinar and corneal epithelial cells. This glycoprotein has
a highly restricted glandular distribution, and this highly
restricted expression pattern in combination with its functional
attributes are evidence for its putative autocrine/paracrine
differentiative role in the lacrimal gland and neighboring ocular
system. Since the gene promoter regulating lacritin gene expression
is the most specific of any previously described lacrimal gland
gene, the regulatory elements of this gene could be used to express
other gene products in the eye. In particular, the lacritin gene
promoter can be operably linked to a wide variety of exogenous
genes to regulate the expression of the gene products to the
lacrimal gland and/or used as gene therapy to treat Dry Eye
syndromes.
[0071] Alternatively, recombinant constructs comprising the
lacritin promoter can be used to transform host cells in vitro as a
means of screening for agonist and antagonist of lacritin function.
In accordance with one embodiment the lacritin gene promoter is
linked to a heterologous gene and reintroduced into a patient to
provide gene therapeutic treatment of Dry Eye syndromes. Simply
stated, the promoter could be used to artificially drive the
synthesis and secretion of tear proteins in patients for which the
normal gene control of these proteins may have been lost.
[0072] Physiological experiments using recombinant lacritin
generated by E. coli suggests that it is likely a growth factor.
Lacritin stimulates calcium signaling in human corneal epithelial
cells and in mouse lacrimal acinar cells. It stimulates tyrosine
phosphorylation in rat lacrimal acinar and human salivary ductal
cells, and it enhances the quantity of tear proteins released from
the same acinar cells that produce it.
[0073] The full length `lacritin` cDNA has been cloned from a human
lacrimal gland library (SEQ ID NO:2), and the corresponding genomic
gene (SEQ ID NO: 1) has been cloned and sequenced, including 5.2 kb
of upstream and 2.8 kb of downstream genomic sequence. A mouse
homologous gene has also been partially RT-PCR cloned, and this
isolated mouse lacritin gene sequence has 99% identity to the human
sequence. Expression of lacritin is remarkably restricted.
Fifty-two different tissue polyA+ or total RNA's were screened and
lacritin mRNA was detected only in lacrimal (very abundant),
salivary (weak to moderate) and thyroid (weak) glands. A review of
the literature suggests that this level of transcriptional control
is unmatched by any other known lacrimal protein.
EXAMPLE 1
Isolation of the Lacritin Gene
[0074] cDNA and Genomic Cloning of Lacritin
[0075] Duplicate filters containing plaques (5 .times.104 per
filter) from each of ten sublibraries of a human lacrimal gland
cDNA library (Dickinson & Thiesse, 1995) were prehybridized at
42.degree. C. for 4 hr in 5.times.Denhardt's, 6.76.times.SSC, 10 mM
sodium phosphate, 1 mM EDTA, 0.5% SDS and 182 .mu.g/ml salmon sperm
DNA, and then hybridized overnight at 42.degree. C. with one of two
overlapping 23-mer oligonucleotides (`s1` [AGCTGGGGCACAGGCACCCGCAC;
SEQ ID NO: 11 ] and `S2` [GGGGTTCTGGGGCTGCAGCTGGG; SEQ ID NO: 12])
that had been end-labeled with [32P]gATP 7000 Ci/mmole (ICN, Irvine
Calif.) and purified. Final wash conditions were 2.times.SSC
(45.degree. C.), corresponding to 29.5.degree. C. less than the S1
or S2 Tm (74.5.degree. C. in 2.times.SSC for both). Plaques
positive in both filters were picked and rescreened three times in
duplicate with each oligonucleotide, giving rise to forty-seven
clones. Each was subsequently reanalyzed at increasing wash
stringency (-29.5, -24.5, -19.5, and -14.5.degree. C. Tm). Inserts
were excised into pBluescript and both strands sequenced via a
Prizm 377 DNA Sequencer (Perkin-Elmer, Branchburg, NJ; University
of Virginia Biomolecular Research Facility). Of identical clones,
most common was a novel sequence lacking homology to BM180 (BestFit
quality=16, vs random quality of 17.+-.2) from which the poly
G-rich S 1 and S2 oligonucleotides were derived. Predicted was a
417 bp open reading frame, whose expected protein product was
designated `lacritin`, in keeping with its lacrimal gland
expression. Lacritin insert was subsequently used to screen a human
P1 genomic library (carried out by Genome Systems Inc; St. Louis
MO) and three identical clones were obtained, as determined by
restriction digestion and Southern analysis. The largest
lacritin-positive fragment (12.4 kb) was subcloned intact into
pBluescript and both strands were completely sequenced. Alignment
and analyses (Kumar et al, 2000) of cDNA and genomic sequence was
primarily with Unix-based (Gelstart, Gap) and web-based (FASTA,
BestFit, Gap) Genetics Computer Group (Madison WI) software using
default settings and E values (FASTA) restricted to 5 or less.
Genomic exon searching and identification of splice sites was
facilitated by the Baylor College of Medicine Human Genome
Sequencing Center web site. All nucleotide sequences have been
submitted to the GenBank/EBI Data Bank with accession numbers
af238867 (cDNA) and ay005150 (genornic).
[0076] Northern Analysis
[0077] Human lacrimal and submandibular glands were obtained during
autopsy through the Southern division of the Cooperative Human
Tissue Network within 18 hours of death and most within 8 hours to
minimize autolytic degradation. The tenets of the Declaration of
Helsinki were followed and informed consent and full IRB approval
were obtained. Donors were without known systemic bacterial or
viral infections, and tissues were normal as determined from cause
of death, pathology reports and in most cases histological
examination. Tissues were snap frozen in liquid nitrogen after
removal and stored at -85.degree. C. until used for RNA
preparation. Total RNA was extracted from 100-300 mg of tissue
using a commercial version of the acidified guanidine
thiocyanate/phenol method (RNazol B, Tel-Test, The Woodlands, TX).
Purified RNA was dissolved in diethylpyrocarbonate-treated water,
and the concentration and purity determined from the A260/280
absorption values. A ratio close to 2.0 was considered acceptable.
RNA integrity was initially determined by electrophoresis of
ethidium bromide-complexed RNA samples in a gel containing 0.22M
formaldehyde. Samples that did not show prominent 28S and 18S rRNA
bands in a 1:1-2:1 ratio under UV light were rejected. For
blotting, RNA (5 .mu.g/lane) was separated on a 0.8% agarose gel
under denaturing conditions (Laurie et al, 1989) and transferred to
nitrocellulose. Also assayed were two purchased (cat # 7756-1 and
7751-1; Clontech Labs, Palo Alto CA) Northern blots with multiple
human fetal and adult poly A+RNA's and a dot blot (cat # 7770-1;
Clontech Labs) containing fifty different human poly A+RNA's
together with control RNA's and DNA's. Blots were hybridized with
[32P]-labeled lacritin insert, washed in 0.1.times.SSC, 0.1% SDS
(Northern) or 2.times.SSC, 0.1% SDS (dot blot) at 55.degree. C.,
and exposed to X-ray film. Dot blots were then quantitated using
NIH Image by measurement of pixel gray values of individual
dots.
[0078] PCR Analysis and Chromosome Mapping
[0079] Alternative splicing was examined by RT-PCR using human
submandibular or lacrimal total RNA and initial priming with oligo
dT, or in a gene specific manner with lacritin reverse primer
CGCTACAAGGGTATTTAAGGC (SEQ ID NO: 13) corresponding to nucleotides
523 to 503 from lacritin cDNA). Subsequent amplification with
lacritin forward primer ACTCACTCCTCATCCCAAAG (SEQ ID NO: 14; from
exon 1; lacritin cDNA nucleotides 32 to 51) and reverse primer
TTTTCAGCTTCTCATGCCC (SEQ ID NO: 15; from exon 5; lacritin cDNA
nucleotides 480 to 462) involved denaturation for 2 mnin at
94.degree. C., thirty cycles of amplification (94.degree. C. for 30
sec, 52.degree. C. for 30 sec & 72.degree. C. for 1 min), and a
final cycle for 5 min at 72.degree. C. PCR product was analyzed in
agarose gels.
[0080] For FISH mapping (Genome Systems; St. Louis, Mo.), lacritin
genomic DNA was labeled with digoxigenin dUTP by nick translation
and hybridized (50% formamide, 10% dextran, 2.times.SSC) to
metaphase chromosomes from PHA-stimulated human peripheral blood
lymphocytes. Following washes, specific labeling was detected with
fluoresceinated antidigoxigenin antibodies and DAPI, and examined
in a Nikon Labophot microscope. A total of eighty metaphase cells
were analyzed with sixty exhibiting specific labeling. Confirmation
was achieved by double labeling using a 12q15 marker, and by
comparison with human genome project draft sequence. Photographs
were taken on a Nikon AFX at a final magnification of
1,435.times..
[0081] Results:
[0082] Lacrimal acinar cells are polarized exocrine secretory cells
containing some mRNA's that are remarkably under-represented in
gene data banks and may code for a rich array of differentiation
factors--a presumption underlying the paired oligonucleotide
screening of a little used human lacrimal gland cDNA library. Among
the clones identified by this approach was a novel cDNA sequence
(SEQ ID NO: 2) represented by several independent clones and
corresponding to a 760 bp transcript and the corresponding amino
acid sequence (SEQ ID NO: 4). The secreted gene product of this
lacrimal gland-specific transcript was designated `lacritin`. The
lacritin nucleic acid sequence contains a 417 bp open reading frame
that predicts a 14.3 kDa hydrophilic protein core with a 19 amino
acid signal peptide giving rise to a mature secreted core protein
of 12.3 kDa with an isoelectic point of 5. Noteworthy is a
moderately high level of glycosylation with six putative
O-glycosylation sites between residues 52 and 64, and a single
N-glycosylation site near the C-terminus, indicating that lacritin
is a moderately well-glycosylated core protein much like the
neuroglycan C glycosaminoglycan binding domain and fibulin-2 amino
globular domain to which lacritin bears partial homology. Northern
Blot analysis indicates a high level of lacrimal gland
specificity.
[0083] In FASTA searches of the primate database, partial homology
is detected with the glycosaminoglycan binding region of human
neuroglycan C (32% identity over 102 amino acids; BestFit
quality=83 versus 37.+-.5 when lacritin sequence was randomized)
and with the `cysteine-free`, possibly mucin-like, amino globular
region of human fibulin-2 (30% identity over 81 amino acids;
BestFit quality=81 versus 38.+-.5 for random). Although all three
are rich in O-glycosylation, positioning of serine and threonine is
not strictly shared; and both lacritin and fibulin-2 lack
glycosaminoglycan binding sites. Neuroglycan C (af059274) is a
component of brain extracellular matrix (anchored by transmembrane
domain; Yasuda et al, 1998). Fibulin-2 (x89494) is widely dispersed
in basement membranes and stroma of embryonic and adult tissues
(Sasaki et al, 1999). Searches of non-primate databases pointed to
modest homologies with T. Cruzi mucin-like protein (af036464;
BestFit quality=78 versus 46 .+-.10); P. falciparum merozoite
surface antigen 2 (u91656; BestFit quality=76 versus 53.+-.6) and
P. Taeda putative arabinogalactan protein (af101791; BestFit
quality=74 versus37 .+-.4).
[0084] No matching or homologous EST's were detected, in keeping
with lacritin's abundance in human lacrimal gland and restricted
expression elsewhere. Northern analysis revealed a strong 760 bp
lacrimal gland message, and weaker submandibular and thyroid gland
messages of the same size. No message was detected in human adult
adrenal gland, testis, thymus, pancreas, small intestine or
stomach; nor in human fetal brain lung, liver or kidney. Similarly,
in a commercial dot blot of fifty different human tissue poly
A+RNA's that excluded lacrimal gland, lacritin expression was found
only in submandibular gland (`salivary gland`), and to a lesser
degree in thyroid. The lacritin coding sequence was subcloned into
pET-28b and pcDNA3.1/myc-His(+)C. to generate recombinant bacterial
and mammalian (293-T cell) lacritin, respectively. Both forms of
lacritin displayed anomalous migration in SDS PAGE.
EXAMPLE 2
[0085] Characterization of Lacritin Expression and Function
[0086] Preparation of Recombinant Lacritin and Anti-lacritin
Antisera
[0087] Full length lacritin CDNA was subcloned in frame into
pET-28b (Novagen, Madison Wis.), with orientation confirmed by
completely sequencing through the insert. Recombinant His-tagged
lacritin was then generated by IPTG-induction of BL-21 transformed
cells, and purified from media on Talon (Clontech; Palo Alto CA)
resin using standard denaturing procedures. Required use of
denaturing conditions for the binding step is presumed to reflect
His tag inaccessibility due to folding in the absence of
glycosylation. After elution, lacritin was extensively dialyzed
versus PBS, and the His tag was removed by thrombin cleavage.
Protein quality was assessed by SDS PAGE and Western blotting with
anti-His antibody (Santa Cruz Biotechnology; Santa Cruz CA).
Lacritin displays anomalous mobility in SDS PAGE. Lack of
contaminating bacterial lipopolysaccharide was confirmed by the
limulus amebocyte lysate assay (MLR Reference Lab; Cypress Calif.).
For analytical comparison, small amounts of mammalian lacritin were
expressed in 293T cells using pcDNA3.1/myc-His(+) (Invitrogen,
Carlsbad Calif.) containing lacritin insert, and then purified
under native conditions.
[0088] Anti-bacterial lacritin antiserum was subsequently prepared
in rabbits (Covance Research Products, Denver PA), and assessed by
ELISA (1/1000 dilution) using recombinant bacterial lacritin (4
.mu.g/ml) as coat and preimmune serum (1/1000) as control. For
immunohistochemistry, sections of zinc formalin-fixed,
paraffin-embedded human tissues and a human tissue microarray were
deparaffinized and rehydrated, and microwave heated (20 min in 10
mM citrate buffer, pH 6.0) to expose antigen. Endogenous peroxidase
was blocked, and then immunodetection was performed using the
avidin-biotin-peroxidase complex method (Vectastain Elite kit,
Vector Laboratories, Burlingame, Calif.) after incubation with
anti-lacritin antiserum or preimmune serum (1/1000) for one hour at
room temperature. Sections were counterstained with hematoxylin,
placed in cupric sulfate, and then immersed in lithium
carbonate.
[0089] Cell Function Analysis
[0090] Freshly isolated rat lacrimal acinar cells, and HSG (human
salivary gland) ductal and HCE (human corneal epithelial) cell
lines were used to study lacritin function. For secretion studies,
rat acinar cells were plated serum-free overnight on wells
co-coated with 0.05 .mu.M laminin 1 (to ensure adhesion) and 0 to
20 .mu.M lacritin, or alternatively with laminin-1 (0.05 .mu.M) and
treated the next day with serum-free medium containing 0 to 162
ng/ml of soluble lacritin for four hours. Unstimulated and
stimulated (carbachol 10-4M/VIP10-8M) secretions were then
collected, assessed (peroxidase assay) and normalized to .mu.g
cellular DNA. To study tyrosine phosphorylation, overnight
serum-free cultures of both rat lacrimal acinar and HSG cells were
washed and treated with 10 ng/ml of soluble lacritin for 0.5, 2.5,
10 and 30 min. Py(20) anti-phosphotyrosine antibody
immunoprecipitation of cell lysates was then examined in Western
blots of 7% SDS PAGE gels using Py(20) and ECL for detection.
Calcium signaling in human corneal epithelial cells was similarly
carried out in serum-free culture (Trinkaus-Randall et al, 2000;
Klepeis & Trinkaus-Randall, in preparation). HCE cells were
grown to confluency on glass coverslips in keratinocyte media (Life
Technologies, Rockville Md.) containing bovine pituitary extract
(30 .mu.g/ml), EGF (0.1 ng/ml) and penicillin/streptomycin, and
rendered quiescent 18 hrs before loading with Fluo-3AM (2 .mu.M;
Molecular Probes, Eugene Oreg.) at 37.degree. C. for 30 min. Using
an inverted Zeiss 510 LSM for visualization, 50 sec baseline images
were first recorded. While the laser was running, lacritin was
added (final concentration 4 and 40 ng/ml) and the response
continually monitored every 786 msec for a minimum of 200 sec.
[0091] ECM Binding Studies
[0092] Binding studies were carried out in 96 well plates coated
with 10 .mu.g/well of collagen IV, laminin-1, entactin/nidogen-1,
collagen I, fibronectin, vitronectin, EGF, heparin or BMS (Matter
& Laurie, 1994). Wells were washed, blocked (PBS-T), incubated
with 0-30 nM lacritin (in PBS-T containing 1% BSA) for 1 hr
(4.degree. C.), washed and detected with anti-lacritin antibody
(1/1000) by ELISA.
[0093] Results:
[0094] Antibodies prepared against bacterial lacritin were applied
to sections of human lacrimal and salivary glands and to tissue
microarrays containing formalin-fixed, paraffin embedded sections
of 75 different human tissues and organs (see Table I).
Immunoreactivity was clearly observed in secretory granules of
acinar cells in lacrimal and major and minor salivary glands, but
was not apparent in other epithelia or stroma. Presence in thyroid
was equivocal (Table I). Frequency of acinar cell staining was high
in lacrimal gland, whereas only scattered salivary acinar cells
were reactive. Immunoreactivity was also apparent in secretions
within lumens of lacrimal and salivary ducts. By ELISA, lacritin
was detected in human tears and to a lesser extent in saliva
1TABLE I Restricted Immunolocalization of Lacritin in Human
Organs.sup.a adrenal - esophagus - parathyroid - small - medulla
intestine adrenal - gall- - parotid gland + spinal - cortex bladder
cord appendix - ganglia - periph. nerve - spleen - bladder - heart
- pituitary gland - stomach - bone/marrow - kidney - placenta -
subman ++ gland brain - lacrimal ++++ prostate - testis - gland
breast - liver - testes - thymus - bronchus - lung - minor salivary
+ thyroid ? gland cerebellum - lymphatics - sem vesicle - uterus/ -
vagina colon - ovary - skel muscle - epididymis - pancreas - skin -
.sup.arelative intensity; not all tissues shown
[0095] Lacritin function was assessed in serum-free cultures of
lacrimal acinar, salivary ductal and corneal epithelial cells using
secretion (acinar), proliferation (ductal), tyrosine
phosphorylation (acinar, ductal) and calcium signaling (corneal
epithelial) assays. Freshly isolated rat lacrimal acinar cells were
plated on increasing amounts of lacritin (with a constant small
amount of laminin 1 to ensure adherence), or on laminin-l-coated
wells in which lacritin was added to the medium. Both coated and
soluble lacritin enhanced unstimulated secretion in a
dose-dependent manner (see FIG. 1), but no effect was observed on
the stimulated secretory pathways activated by the agonists
carbachol and VIP. These results suggest an autocrine or paracrine
role, possibly via receptors on the luminal acinar cell surface. As
lacritin flows from acini, it contacts ductal epithelial cells and
finally the corneal epithelium.
[0096] Quiescent human submandibular ductal (`HSG`) cells were
cultured in serurn-free media containing increasing amounts of
lacritin and cell proliferation was studied. The lacritin cultures
looked healthier; after four days, a dose-dependent increase in
ductal cell number was apparent (see FIG. 2a) that reached a level
more than twofold that of the BSA (10 ng/ml) negative control (see
FIG. 2b). The same level of lacritin promoted the transient
tyrosine phosphorylation of a 48 kDa band in both HSG and rat
lacrimal cells.
[0097] Next, calcium signals in human corneal epithelial cells were
examined. Whereas the basal level of signaling was negligible, the
addition of lacritin resulted in rapid and sustained calcium waves
that propagated throughout the cells. Wave onset preceded that of
the usual response to epidermal growth factor (20-40 sec), and the
amplitude of the response depended on the concentration of
lacritin. To ensure that bacterial lipopolysaccharide (a possible
contaminant of recombinant protein preps) was not involved, samples
were tested in the limulus amebocyte lysate assay; and no
lipopolysaccharide was detected (<0.05 EU/ml). Finally, the
ability of lacritin to bind the tear film components fibronectin or
vitronectin was examined ; as well as constituents of the
periacinar basement membrane that might harbor small amounts of
lacritin not detectable by the immunohistochemical procedure.
Lacritin displayed a remarkable avidity for fibronectin and
vitronectin, and there was a strong basement membrane binding
attributable to collagen IV, nidogen/entactin and laminin-1-similar
to that observed for fibulin-2 (Sasaki et al, 1995). No binding was
observed to collagen I, EGF or heparin.
[0098] The rather broad lacritin lacrimal gland message was
suggestive of alternatively spliced forms, or RNA degradation. The
same was not true for submandibular gland in which a discrete, but
much less intense signal was apparent. To address this issue and to
gain information on how the lacritin gene is arranged, a 12.4 kb
genomic fragment was sequenced, the largest lacritin-positive
fragment readily obtainable from the lacritin genomic clones. The
gene consists of five exons preceded by a predicted promoter
sequence 109 to 59 bp upstream of the translation start site
(promoter score=1.0; NNPP/Eukaryotic). Exon 1 encodes the complete
signal peptide and includes 38 bp of 5' untranslated sequence. Exon
3 contains sequence for all putative 0-glycosylation sites. The
predicted N-glycosylation site is formed at the exon 4/exon 5
splice junction. Exon 5 includes 53 bp of 3' untranslated sequence.
Three potential polyadenylation sites are detected 367, 474 and 534
bp downstream of exon 5, the first of which would be in keeping
with a 760 bp transcript. Sequences at exon-intron boundaries all
conform to predicted splice donors or acceptors, with the exception
of the exon 4 splice acceptor. Intronic sequences revealed common
intronic repeat elements. Also independently discovered on a
separate genomic fragment was a lacritin pseudogene lacking 38 bp
of 5' exon 1 sequence.
[0099] To examine possible alternative splicing, RT-PCR was used
with submandibular or lacrimal gland cDNA as template and forward
and reverse primers from exons 1 and 5, respectively, each
including untranslated flanking sequence. A single PCR product was
detected in both organs whose size (449 bp) was in keeping with
transcription from all five exons without alternative splicing.
FISH revealed that the lacritin gene is located on chromosome 12, a
result confirmed by double labeling with a probe for 12q15.
Measurement often specifically labeled chromosomes located the
lacritin gene approximately 16% of the distance from the centromere
to the telomere of 12q, an area that corresponds to 12q13. Also
found on 12q13 is a rare genetic alacrimia known as Triple A
Syndrome. Attempted PCR using lacritin genomic primers and BAC
templates spanning the triple A syndrome region failed to produce
PCR product. The lacritin gene is partially included in draft
sequences AC068789.4, AC025686.2 and AC025570.6 pointing to a 12q13
location approximately 65.1 to 65.9 Mbp from the centromere.
[0100] Discovery of lacritin developed from the hypothesis that
multiple extracellular factors trigger glandular differentiation,
particularly growth factors and components of the surrounding
extracellular matrix. Indeed, partial or failed acinar formation
has been reported in mice lacking the TGFb superfamily members or
receptors, ErbB4, the progesterone receptor, the extracellular
matrix glycoprotein osteopontin, EGF receptor (with TGFa and
amphiregulin), fibroblast growth factor receptor 2 (IIIb), or the
growth factor FGF-10. Linking such factors to the secretory
function of acinar cells in culture has proven more complex.
Nonetheless, it is clear that the periacinar mesenchymal and
hormonal environment affect glandular development and function, and
that both autocrine and paracrine regulation play important roles.
Most delicate are primary cultures of freshly isolated exocrine
cells, particularly lacrimal acinar cells that functionally
dedifferentiate in the absence of lacrimal-1 and lower molecular
mass factors derived from the extracellular matrix and
elsewhere.
[0101] Introduction of recombinant lacritin to cultures of lacrimal
acinar, salivary ductal and corneal epithelial cells provided
interesting functional insights. Lacrimal acinar cells displayed
enhanced unstimulated (but not stimulated) secretion and rapid
tyrosine phosphorylation of a 48 kDa protein. Ductal cells
phosphorylated the same 48 kDa band and were proliferative. A rapid
and sustained calcium transient was noted in comeal epithelial
cells. Thus all cell types contributing to or benefiting from
lacritin outflow appear to be lacritin-inducible, whereas controls
were negative and there was no evidence of contaminating bacterial
lipopolysaccharide (known to be proliferative in immune cell
cultures). How lacritin acts remains to be elucidated. Possibly a
common receptor(s) is mediatory, ligation of which may be jointly
linked to tyrosine phosphorylation and calcium release as in neural
retina where tyrosine kinases have been associated with
capacitative calcium entry and inositol-3-phosphate induced release
of intracellular calcium stores. Alternatively, lacritin signaling
in the three cell types may differ. Lacrimal acinar, ductal and
corneal epithelial cells perform strikingly different functions.
Although some intracellular signaling machinery may be common,
others are unique, and some common machinery may be put to
different use. Calcium signaling in lacrimal acinar cells is most
frequently a downstream effect of muscarinic receptor ligation that
mediates the release of tear proteins by the stimulated secretory
pathway, a pathway apparently unaffected by lacritin. Yet,
subtleties in calcium amplitude, frequency and localization,
dependent on the nature and dose of the agonist, can have
dramatically different effects. Contrasting lacritin is BM180, a
periacinar basement membrane constituent that appears to act only
on the stimulated secretory pathway. Balancing the amounts of
available lacritin and BM180 may offer a simple mechanism by which
secretory capacity in adult and developing glands may be
controlled.
[0102] Immunolocalization of lacritin in secretory granules, in
secretory content of ducts and in tears was extended by binding
studies revealing a remarkable affinity for tear constituents
fibronectin and vitronectin. Though not immunodetected elsewhere,
lacritin also bound the common periacinar basement membrane
components nidogen/entactin, collagen IV, and laminin-1; but not
collagen I, EGF or heparin. Similar binding properties have been
reported for fibulin-2 (Sasaki et al, 1995). Although the
significance and precise nature of these interactions remains to be
determined, basement membrane binding is perhaps analogous to
growth factors whose extracellular matrix accumulation, although
functionally potent, is often too low for reliable immunodetection.
Alternatively, basement membrane binding (if any) could possibly
occur secondary to tissue damage.
EXAMPLE 3
[0103] Characterization of the Lacritin Promoter
[0104] The working hypothesis is that lacritin gene activity is
attributable to an atypically restrictive and powerful promoter
working hand in hand with unique enhancer (and possibly repressor)
elements in a milieu of appropriate transcription factors and
co-regulators. Such tissue-specific transcriptional control equals
or exceeds that of the aA-crystallin (lens), rhodopsin (retina),
aldehyde dehydrogenase class 3 and keratocan genes (cornea), and
offers a unique opportunity to initiate a new body of literature on
nuclear management of gene expression in the human lacrimal
gland.
[0105] Mapping of Lacritin Gene Regulatory Elements
[0106] Elucidating how lacritin gene expression is targeted to the
lacrimal gland will be determined as described below to better
understanding lacrimal gene regulation. First of all the identify
the lacritin transcription initiation site(s) will be confirmed
experimentally. Based on computational promoter analysis,
transcription is anticipated to be initiated at a single site
located 69 bp upstream (`-69 bp`; `Neural Network`) of the ATG
translation start site. The `TATA-box`and/or `Initiator` (`Inr`)
elements of the core promoter play an important role in
establishing the start site of transcription in many genes,
particularly those highly expressed. As an example, Inr elements at
+1,+220 (also TATA-box at +190 bp) and +316 bp (intronic) designate
transcription start sites in the human keratocan gene as
experimentally confirmed by primer extension (see Tasheva ES,
Conrad AH, Conrad GW. Identification and characterization of
conserved cis-regulatory elements in the human keratocan gene
promoter. Biochim Biophys Acta. Jul. 24, 2000 ;1492(2-3):452-9);
and a TATA-box figures prominently in transcription initiation of
aA crystallin, rhodopsin, and aldehyde dehydrogenase gene
promoters. If the Neural Network-predicted -94 to -46 bp region
does indeed comprise the lacritin core promoter with transcription
start site at -69 bp (score=1.0), then putative TATA-box and Inr
elements at -52 and -67 bp, respectively should play a key role in
transcription initiation. Alternatively, transcription could begin
at -62 bp, as suggested by `CorePromoter`. Primer extension and RNA
ligase-mediated 5'-RACE will resolve this question.
[0107] For primer extension, advantage will be taken of a 20-mer
reverse primer (`LacP83`) designed by `Prime` (GCG, Madison Wis.)
which is complementary to nucleotides 64 to 83 bp of lacritin mRNA.
As per routine procedure, LacP83 will be end-labeled by
phosphorylation with T4 polynucleotide kinase in the presence of
[g.sup.32P]ATP, annealed with total lacrimal RNA (100 fmol primer
per 10 .mu.g RNA) for 20 min at 58.degree. C., cooled, and then
incubated for 30 min at 41.degree. C. with AMV reverse
transcriptase (Promega, Madison Wis.) in the presence of
deoxynucleotides. Size of newly formed cDNA(s), as analyzed by
denaturing SDS PAGE analysis/radiography, provides sufficient
information to calculate the approximate transcription start site
location(s)-with identification of the 5' terminus(i) determined by
semiautomatic ABI sequencing of cDNA from a scaled up
non-radioactive extension reaction and RNA ligase-mediated 5'-RACE.
Primer extension controls will include replacement of lacrimal RNA
with total yeast RNA (or no RNA), and use of an RNA prepared by in
vitro transcription with accompanying primer (Promega, Madison
Wis.) for which primer extension conditions have been previously
established.
[0108] For confirmation, RNA ligase-mediated 5'-RACE (`GeneRacer`-2
; Invitrogen) will be utilized. This is a powerful PCR-based
modification of primer extension. For this purpose, 1-5.mu.g of
total human lacrimal RNA will be treated with calf intestinal
phosphatase (1 U per 10 .mu.l reaction mix) to remove 5' phosphates
from degraded RNA and non-mRNA contaminants. Incubation with
tobacco acid pyrophosphatase (0.5-1 U per 10 .mu.l reaction mix)
eliminates the 540 -CAP structure present only on authentic
5'-ends, and makes possible ligation of a kit-specific RNA
oligonucleotide (`GeneRacer RNA Oligo`) with T4 RNA ligase (5 U per
10 .mu.l reaction mix). Subsequent LacP83-primed reverse
transcription will generate a single strand cDNA. The cDNA will
then be PCR amplified using LacP83 and a primer complementary to
the 5'RNA oligo as primer pair, and sequenced to identify the start
site(s). In negative PCR controls, amplifications will be attempted
in the absence of LacP83 or GeneRacer RNA Oligo or without
template, and if banding or smearing is observed further PCR
optimization will be carried out (ie. use of less template or fewer
PCR cycles or do nested PCR to increase amplicon amount, or use
touchdown PCR).
[0109] I t is anticipated that a single primer extended cDNA band
of 152 (or 145) bp will be observed in keeping with a transcription
start site at -69 bp (or -62 bp) and inclusion of 83 bp from the 5'
end of the primer to the translation start site [69+83 bp (or 62+83
bp)]. This expectation is in agreement with the single transcript
apparent by Northern analysis of human salivary gland. The broader
human lacrimal band has been interpreted as attributable to MRNA
abundance combined with possibly some slight degradation. Although
no alternative splicing has been observed, the possibility of a
second transcript cannot be completely ruled out.
[0110] A luciferase reporter constructs will also be generated and
transfection-based regional mapping of lacritin gene regulatory
elements will be initiated. It is hypothesized that Bayesian
alignment of human and mouse lacritin genes will provide an
excellent foundation for interpretation of reporter construct
activity, and that evolutionary conservation similarly will make
feasible utilization of a rabbit lacrimal acinar cell line as
transfection host-the only immortalized cell line from lacrimal
gland of any species. This exploratory approach will lay the
conceptual groundwork for more detailed studies both in vitro and
in vivo.
[0111] Lacritin's tissue specificity is presumably founded in the
nature and assortment of transcription factor binding modules that
comprise its gene promoter and putative enhancer region(s).
Lens-preferred expression of the aA-crystallin gene, for example,
is governed by a transcription complex of CREB/CREM, aA-CRYBP1, Pax
6, TBP, USF, AP-1 (context of AP-1 important for tissue
specificity) and L-maf that nucleates on the 150 bp aA-crystallin
promoter. Transfected plasmid constructs that artificially position
luciferase or chloramphenicol acetyltransferase expression under
the control of intact or progressively 5' shortened (or mutated)
promoter regions, has been used previously to identify cis-acting
regulatory region of a promoter. The versatile and sensitive
`Dual-Luciferase Reporter Assay System`(Promega) for example
sequentially assays both the transfected gene promoter under
investigation (as manifested by the level of expressed firefly
luciferase) and a co-transfected internal positive HSV-TK control
promoter designed to independently drive expression of a synthetic
sea pansy luciferase with distinct substrate properties at a
constant baseline level (see below). Subsequent investigation in
transgenic mice using b-galactosidase as reporter brings
chromosomal context into play. Recent availability of a rabbit
lacrimal cell line Nguyen DH, Beuerman RW, Halbert CL, Ma Q, Sun G.
Characterization of immortalized rabbit lacrimal gland epithelial
cells. In Vitro Cell Dev Biol Anim. 1999 April;35(4):198-204.) and
genomic cloning of lacritin now open up this line of investigation
to the lacrimal gland field.
[0112] If transcription is indeed initiated at -69 or -62 bp,
upstream genomic constructs spanning -2435 to -10 bp
(`Lacrgen2.4`), -1619 to -10 bp (`Lacrgen1.6`) or -856 to -10 bp
(`Lacrgen0.9`) could include all or most elements necessary for
tissue specific and elevated expression. Preparation of each will
take advantage of parent amplicon `LacrgenInit`(-2960 to -10 bp) to
be generated by PCR from the 12.4 kb lacritin genomic fragment
using reverse primer `LacP-10/Xho I`(-10 to -31 bp) with an Xho I
site incorporated, and forward primer `LacP-2960`(-2960 to -2942
bp). Primer pairs are designed by `Prime` (GCG, Madison Wis.).
Subsequent digestion of LacrgenInit with XhoI, Bgl II/Xho I or Hind
III/Xho I yields fragments Lacrgen2.4, Lacrgenl 16 or Lacrgen 0.9,
respectively with ends suitable for ready ligation (after gel
purification) into the multiple cloning region of pGL3-Basic just
upstream of the promoterless and enhancerless luciferase gene
(luc+).
[0113] A new rabbit lacrimal acinar cell line (Nguyen et al, `99),
that has been cultured for twelve months without difficulty will be
used for the transfection studies. The cells display a strong
epithelial morphology and synthesize secretory component,
transferrin and transferrin receptor. Importantly, they also
express lacritin and are readily transfectable. To carry out
transfections, .apprxeq.80% confluent serum-containing cultures in
96 well plates will be transiently transfected with Lacrgen2.4,
Lacrgen 16 or Lacrgen0.9 in pGL3-Basic plus internal control
phRL-TK plasmid (total of 0.24 .mu.g plasmid/well; 50:1 ratio of
pGL3-Basic to phRL-TK) using .apprxeq.0.8 .mu.l/well LipofectAMINE
2000 reagent (Invitrogen Life Technologies). 48 hours later,
cultures will be gently washed three times in PBS, lysed for 15 min
in 1.times. `Passive Lysis Buffer`(20 .mu.l/well; Promega), and
assayed for firefly luciferase upon addition of0 `Luciferase Assay
Reagent II`(100 .mu.l/well) in an L-Max 96 well plate luminometer
(Molecular Devices, Menlo CA; online with computer). Readings are
zeroed to similarly treated wells containing lysate of cells not
transfected. Subsequently, `stop & Glo Reagent`(100 .mu.l/well)
is added for assay of sea pansy (Renilla) luciferase. Inclusion of
identically transfected human 293 cells will serve as a negative
control, whereas Araki-Sasaki human corneal epithelial cells
(HCE-T) and HSG human salivary cells (both secrete lacritin) are
suitable positive controls. Optimal lacrimal LipofectAMINE
transfection, and `Bright-Glo` luciferase assay conditions
(Promega), will take advantage of the pGL3-Control vector whereby
transfected cells benefit from luc+expression under SV40 promoter
and enhancer control. It is expected that transfection efficiency
will be 75-90%, and that one of Lacrgen2.4, Lacrgen1.6 or
Lacrgen0.9--likely Lacrgenl.6 or LacrgenO.9--will best define the
minimal sequence required for lacritin promoter activity.
[0114] This course of investigation offers a logical starting point
for the generation and testing of Lacrgen2.4, Lacrgen1.6 or
Lacrgen0.9-derived constructs progressively shortened 5' by nested
deletion, an approach applied to the genomic sequencing of the
lacritin gene and flanking regions. Making this possible are single
Kpn I and Sac I sites just upstream of each insert in the
pGL3-Basic multiple cloning region, and lack of any internal Kpn I
or Sac I sites in Lacrgen2.4, Lacrgen1.6 or Lacrgen0.9-the latter
as determined by Map (GCG). Thus when digested with Kpn I and Sac
I, a linear plasmid will be generated in which the Kpn I end is
exonuclease III resistant (3' protruding) and the Sac I end (3'
recessed) is sensitive. Proximity of Lacrgen2.4, Lacrgen1.6 or
Lacrgen0.9 to the Sac I site makes them sensitive to exonuclease
shortening. To carry this out, 2 .mu.g of plasmid construct is Kpn
I and Sac I digested. After enzyme inactivation (10 min at
70.degree. C.) and cooling on ice, linear plasmid in exonuclease
III buffer is treated with exonuclease III in a final volume of 40
.mu.l at 25.degree. C. or 15.degree. C. such as to achieve
successive 50-100 bp deletions at 3 min intervals. 2 .mu.l aliquots
of each 3 min time point are removed to tubes on ice containing Sl
nuclease. After all timed aliquots have been taken, plasmid digests
are removed from ice, incubated at room temperature for 30 min for
Sl nuclease digestion of overhangs, heat inactivated,
recircularized by blunt end ligation in the presence of T4 DNA
ligase, examined in agarose gels and transformed into competent
cells with ampicillin selection. Plasmid preps of each are then
applied to the transfection (with internal control plasmid) of
lacrimnal acinar cells, and assessment of luciferase expression.
Sequence CWU 1
1
15 1 12354 DNA Homo sapiens exon (5194)..(5251) 1 acatttttaa
aattttttca ctcattgctt tgtctttaca cctccccgat ggccaaggtg 60
gaagatcgga ggcatcacag gagtgtggca gagcttgtgc aggccacagg gcttggcaga
120 gaagacaagc catgtcgagc acagcagcca gggtagaatg gccctcggag
atcaacgtgt 180 gcctgtgtct ccaatgcagg agcagtctac cctaaatagt
ccatgtcaat tcctcccttt 240 ggagtctctg cttccccacc agcccccaga
acatggccta acacacaggg aggggaatga 300 ggaaaagaca ttcatcacag
ttcagacagg aagtggtgta tcagtggaga ggtccaagta 360 gaaaacaaat
ggcacactca ggagggctta tatatatata taaatacttt aagttctagt 420
gtacatgtgc acaatgtgca ggtttgttac atatgtatac atgtgccgtg ttggtttgct
480 gcacccatta actcatcatt taccttaggt atttctccta atgctatccc
tcccccatcc 540 ccccacccca caacaggcct cggtgtgtga tgttccccac
cctgtgtcca agtgttgtca 600 ttgttcaatt cccacctatg agtgagaaca
tgtggtgttt ggttttctgt ccttgcgata 660 gtttgctcag aatgatggtt
tccagcttta tccatgtccc tacaaaggac atgaactcaa 720 ccttgtttat
ggctgcatag tattccatgg tgtatatgtg ccacattttc ttaacccagt 780
ctatcattga tggacatttg ggttggttcc aagtctttac tattgtgaat agtgccacaa
840 taaacataca tgtgcatgca tctttatagt agcatgattt ataatccttt
gggtatatac 900 ctagtaatgg gatctttggg ttaaatggta tttctagttc
tagatccttg aggaatcgcc 960 acactgtctt ccacaatggt tgagctagtt
tacactccca ccgatggtgt aaaagcattc 1020 ctatttctcc acatcctctc
cagcacctgt tgtttcctga ctttttaatt attgccattc 1080 taactactgt
gagatgatat ctcattgtgg ttttgatttg catttctctg atggccagtg 1140
atgatgagca ttttttcatg tgtctgctgg ctgcataaat ctcttctttt caaaagtgtc
1200 tgtccatatc ctttgcccac tttttgatgg ggttgtttga ttttttcttg
taaatttgtt 1260 taagttcttt gtagattctg gatattagcc ctttgtcaga
tgggtagatt gcaaaaattt 1320 tctcccattc tgtaggctgc ctgttcactc
tgatggtagt ttcttttgct gtgcagaagc 1380 tctttagttt aattagatcc
catttgtcta ttttggcttt tgttgccatt gcttttggtg 1440 ttttagtcat
gaagtacttg cccatgccta tgtcctgaat ggtattgccc aggttttctt 1500
ctagggtttt tatggtttta ggtctaacat ttaagtcttt aatctatctt gaattaattt
1560 ttgtataagg tgtaaggaag ggatccagtt tcagctttct acatatggct
agccagtttt 1620 cccagcacca tttattaaat aaggaatcct ttccccattt
cttgtttttg tcaggtttgt 1680 caaagatcag atggttgtag atgtgtggtg
ttatttctga ggcctctgtt ctgttccatt 1740 ggtctatatc tgttttggca
ccagtaccat gctgttttgg ttactgtaag ctggtagtat 1800 agtttgaagt
caggtaatgt gatgcctcca gctttgttct ttttgcttag gattgtcttg 1860
gcaatgcagg cccttttttg gttccatatg aactttaaag tagttttttc cacttctgtg
1920 aagaaagtca ttggtagctt gatggggatg gcattgaatc tctaaattac
cttgggcagt 1980 atggccattt tcacgatatt gattcttcct atccaagagc
atggaatgtt cttccatttg 2040 tttgtgtcct cttatttcat tgagcagtgg
tttgtggttc tcaggagggc tttttttaaa 2100 aaaggttctt taaggaaaaa
tgtatctatt atttacagag attcaagtat ggactagcaa 2160 cagcaggaag
tctgaagcag cgtgttgggg ggtgggggtg aataatgtca cctgacaaca 2220
agagatggcg ctctgaaaca gggaccagac agaagctgca gtcagagaga aacagccatt
2280 gccacaccat gctgcattaa gtatccgtgg ctgtaaaaat tattcaaaca
tttggtggct 2340 tcacacatga tttcttatcc acagttgtta tggatcagca
gtccaggtac agatgcgccc 2400 tcagacacag agtctctaat gaggctgcag
tcaaggtatc atcgaggctg cggtcacctc 2460 aaaactcagc tgaggaaaga
cccacttcca agctcagtca cgtggtagct ggcagggttg 2520 agttcctcac
agatagctgg actaagggcc tcggctcctt actggctaca ggctggaggc 2580
tgtcctcggt ttcttgccac gtaggtctct ccacaggaca gcctcacaca tggccactca
2640 cttcatcaga gcaagctgag aagatccaga gacagagtgt gtgtaaacaa
gactaaagtc 2700 atagtctcag aactgactac ctcatcactt tgccatcttc
tatttgttag gagtgaatct 2760 cgagacccag cccagttaag cagaggaaat
tacagcaggg caccaatgcc aagacgtagg 2820 catcaccagg agctatctct
gaagtcgtcc taccacacat gccaaagcaa gagagggagc 2880 aggaggaaca
aatgccttga tctctccctt ttcccaccct cccatctccc acctgtgtct 2940
cccattggcc aaactcaact cgaagctaga gggcatggga gcccaggtga tacagcccat
3000 agaggcatcc ttccagacca gtgcagagat gagagagaat aaacctgagg
cagaggggag 3060 agaggtacaa cgagcagccc atgagacaag acacagatca
gcgaactgac agtcatcatg 3120 gaggtcagat aacctagaaa acaagacatc
acacagagca gcccctcacc catatccatg 3180 aggtccgtca agacattgca
cagagcagcc tctcacccat atccatgagg tctgcctctt 3240 taatctatgc
acccaattct ttcccaggct ctataatttg gggtaacatt tctctgggcc 3300
ctgttataga ataatgaaaa ttttctataa aacaaatgtc cttactttca cctcatccct
3360 atttatgtaa atgcttgcct ctttaattac tgaggccagg aggaagattt
gggagaggaa 3420 agggctatgc ggtgacattt ggaaagaccc tgctctgtga
cagttctagt gttgacgcca 3480 aagttctcat ttcctcttag aaaagtcttg
tgcaaatagc ataatttgct cctgttgact 3540 tttttaatgt gctcatggag
actgctcggg atctagatct gtttgggatc tgcaggactt 3600 ctccttctgc
agtgtacaca cacgtgcaca cacgtatgtg cacataccca gggcactggt 3660
gccatcaaaa ctttctcttg ttcttcagcc ttccccattc caggtaaggc caccaccacc
3720 ttcaaggctc ccaggcccag accctcaggc aggtagcaat tgccaaggct
ttaatgtccc 3780 accccattaa ttttatcttc ctttatctcc tgaaaagatt
aatgttctaa accctggcac 3840 ccaaaacacc ctcatgttga aaactcttca
ataccccctt ctcacactca tcttcagagc 3900 taccatgagg cagagaagcc
tccggaatca gcccacatgg ggctgggtga atgccaacac 3960 caagcaaggg
gaaagtcaca aattgacatc cagcacctta ttctccaccc ttcagcccct 4020
caactgactc ctgctccacg gcccgttcta ttaatatcta gcatttagca ccagcctgga
4080 caaaaaccta cttggaaaga tggtacaaga accccacaca actccataga
acttcgctgt 4140 ctaaaaaaat gctttgccgt atattatccc acttaatctc
caccactatg ctgtacatag 4200 gagccacaac tcctagacaa caaataaaaa
tcctatcact tttcaaatcc taacattttc 4260 atatgacaaa gccagaactc
aaaatccaga cctctagagt cccagatcag gaaaggaaga 4320 aacgccaagt
caaagagaag cttctttaga ataatctgct tttctggatt attcacacca 4380
tgggtcagct ccccacttga agtcagaacc aagctccaat ttcagtgaac caccatcatg
4440 ctttgaccag gagattctct cagaaatgtg gggtcccatt gagtaggcct
gaagacagag 4500 attgacaggc ctatgtgagc ctggaggagt tctttttagg
ggctggataa tgtcaagaac 4560 agagaacaac tccagagaag gcacacacgc
cttcaaaccc atcccctcat ggggagaaag 4620 cagccaggaa ctcaggcctc
aagtgttcta ggtgtggtct cccaaggaaa cgggctcact 4680 tagtttgggg
aaaccttcaa accctgcact gagtcctatg tagactggga cagaaggtgg 4740
acaatgtaat cccctgagcc ctcaacctcc tcctggagag atgacaagat taagatttct
4800 ctaccagaac cctcaacaga cacatcccag aatctcccca agtgaaatgt
gctctaccta 4860 ccgtccctga gagcccaggg gtgtgaaccc agagggcagg
tgtggtgggg aagggaggag 4920 ggagaaagaa aagggatggc tgggagttag
agaaaggctc ctatccagga cctgcctgca 4980 aggatcccag gtatcagcca
gcccaaccta gcccttgttg acttagcagg tgacagtttg 5040 gggaagaagg
ggaggaggat gcggaagtca cacctctcca ggcttggttc ccattggccc 5100
ttgatatcct taaaagggcc cagcaatttc agcatcctta ttccccagac cttctgcaga
5160 ttctgtggtt atactcactc ctcatcccaa aga atg aaa ttt acc act ctc
ctc 5214 Met Lys Phe Thr Thr Leu Leu 1 5 ttc ttg gca gct gta gca
ggg gcc ctg gtc tat gct g gtgagtatgg 5261 Phe Leu Ala Ala Val Ala
Gly Ala Leu Val Tyr Ala 10 15 cctttcctct gcgccccaca agagtcctcc
cagtccaagg agcccctcac tcctgccttc 5321 acccctctcc tcctctctca
gtgctattct ggtttccctg cctctgcaag tgactcctct 5381 cccagttctc
cacacgtggc ctctgcaccc cactggccag aggaacccag aactctctgg 5441
cctctgcctg ccctcccagc tcatctcctc acacaccatt gtttacccac tatgcctcag
5501 ctacactggc ttctctggtg tcccctgcat gtagttgagc agggtgtccc
ctacacgagg 5561 gtgcccaggc aaggagtggt agaagctaaa atctggccga
cactctactt gccaagcagt 5621 gagcctggcc cctggctgtg tctcttagga
ggaagggatg cctttttttt tttttttttt 5681 ttgagaccga gtctccctct
gttgcccagg ctggagtgca gtggcacgat ctctgctcac 5741 tgcaacatcc
acctcctggg ttccagcgat tatcttgcct cagcctcctg ggtagctggg 5801
actacaggct catgccacct tgcccagcta attttgtatt tttagtagag acggtgtttc
5861 accatgttgg ccaggttggt ctcgaactcc tgacctcagg tgatccgact
gccttggcct 5921 cccaaagtgt tggaattaca ggcgtgagcc accgtgccct
gctgggatgc cttttttgat 5981 ccacagaagc actatttggg ccatgatgat
cctgctgttc cttgaacatc aggatcttcc 6041 ttcttgtcct ttcctcgtct
agaatgcttt ccctctccct ggccccttcc cccaaccaac 6101 tctaatgtca
cctggccaat gatttttcat ctagaaaatc tcagtttaca tataattccc 6161
caaaaaggcc ttccatgcac atgcggaaca aatcagatcc atgtgccctt ctcgcaccag
6221 gctgcacgtt cccttccagc actgtcacac cagccattaa ataatttcgt
aaaaggacag 6281 atgtaagctc tgtcagggca ggggtcttgt ctgccctctt
cagcactgca cctccatctc 6341 ttggcacaga gctttgcata aatgttgtgt
tgaaagaata aagggaatca aggctggggt 6401 ctcaatcctg caaatcgctc
aaatatggcc ccataacccc cacatactgt cctcctccac 6461 cacagaggag
gttgagcccc tctgaccatg gccagctcca tgacagacac ctcagggaag 6521
cctaccaagc caggggccag tcaggaggaa ggcactgttc caagagacat tacacttctc
6581 agaggggaag ttatttcaaa agccacagga gttaaacatc agagagtgcc
ccagtagacc 6641 cgctgatatg gtggaagggc atgtccaacc caaagggaaa
ttgatcccct tctatccatg 6701 agcattccca ggagataagc tttgggaatg
ggaggggagg gtggctcgag taggtccggt 6761 tcggtccttg ctctcatctg
gcatgtttcc cccattgca ga aga tgc ctc ctc 6814 Gly Arg Cys Leu Leu
tga ctc gac ggg tgc tga tcc tgc cca gga agc tgg gac ct 6855 Leu Asp
Gly Cys Ser Cys Pro Gly Ser Trp Asp Leu 25 30 35 gtgagtcctc
ctctccctgc tgccctagcc ctcgttggga aggtttaaca gttagggatg 6915
tgagtggtgg ctgggagaag aagccagtgg gaggagatct ggattctgtg cttggtggta
6975 atggggaggg gcaggtaata taataaagaa ggtggcatgg gttgaaatgg
tacaaggcta 7035 aggacaaaag aggatgaccc agagaggcaa ggacaatagg
gagcatgggg aaaaggttat 7095 tgtgaataaa agggagagaa acatgaggtt
aagtggtaag ggcaatgtct cacatggcat 7155 taataccttc acctgcaaac
acctcccatt actcccaatt ccttagcaag ataaccatta 7215 tctggcctct
aacctcattt tccaacccca ttttccatga ctctctttta tgtcgcaccc 7275
ccatcagcta aattgaactt gtttccattc cccacacatg ccttcgcctg acctcttact
7335 cactgcctgc ccccagggaa gcccctttgg tacatcctct cctgctaaca
tcctgccttc 7395 aagatccagc ttctctatga agtgctcccc gattctcacc
atcccctagt ccaaatcctt 7455 ccccaaccct gcccgctgca ttccaagaga
cacacagcat gcagaaatgc tatctccctt 7515 aagggggcag cgtttaagcc
atatcacttc tgtatcctgg cacccagcac acattaggta 7575 tcctggggcc
ctgcaaccca ttccaaaaga aacaaacact ttcactttgc taaaatccat 7635
caatttgtgc attcaca g cta agc cta atg aag aga tct cag gtc cag cag
7686 Leu Ser Leu Met Lys Arg Ser Gln Val Gln Gln 40 45 aac cag ctt
cac ccc cag aga caa cca caa cag ccc agg aga ctt cgg 7734 Asn Gln
Leu His Pro Gln Arg Gln Pro Gln Gln Pro Arg Arg Leu Arg 50 55 60
cgg cag cag ttc agg gga cag cca agg tca cct caa gca ggc agg aac
7782 Arg Gln Gln Phe Arg Gly Gln Pro Arg Ser Pro Gln Ala Gly Arg
Asn 65 70 75 taa acc ccc tga gtaagtctct gtctctatgc cagatcaaca
acctagaaaa 7834 Thr Pro 80 gtctctggct gcaggcccac atcacacctc
cacgcacaga gataagcctg gtgagaagca 7894 ggtagactca aacagctgaa
cacaaaggca caaatgggat tgtgcattgc acccacacac 7954 aacgttttca
caatagtaga tgttgcagcc tgcacaatac atggtttctg tcctggctca 8014
cacaacttcc tatgagagaa gtgctggagc cctcagcaaa acttctgcac tttaggactt
8074 tctgtaggga tgatgtcctg ggtggagtgg gggtgggggg cgggtgcagg
tggggcaatg 8134 cagagttctc tttaaatgag gtgatttttc tgctgatgtg
attgttctgc tccaaaatta 8194 gaa tcc ata gtg gag aaa agt atc tta cta
aca gaa caa gcc ctt gca 8242 Glu Ser Ile Val Glu Lys Ser Ile Leu
Leu Thr Glu Gln Ala Leu Ala 85 90 95 aaa gca gga aaa gga atg cac
gga ggc gtg cca ggt gga aaa caa ttc 8290 Lys Ala Gly Lys Gly Met
His Gly Gly Val Pro Gly Gly Lys Gln Phe 100 105 110 atc gaa a
gtgagtgcat cccaaggcaa ggctttgtgg gaatgagaat actcaccacc 8347 Ile Glu
115 caccatccgg gggtgggata tgggacagaa cttgccccca tttccacctc
acatatgaga 8407 cttggaattg ccacagcccc tgctgttgaa gacccctcac
tttgtgcttt catatgtttc 8467 caatttctca tccagattca aattgccagc
tgggcacggt ggttcacgcc tgtaatccca 8527 gcactttggg aggccaaggc
aggtggatcg cttgagccca ggagttcaag actagcctgg 8587 gcaacatggc
gataccccat ctctacaaaa aaatacaaaa attagccagg cgtggtggca 8647
catgcctgta gtcccagcta cttgggaggc taaggtggga ggatcacctg agcccgtgag
8707 gcagagattg cagtgggccg agattgtgcc actgcactcc atcctgggtg
acagagaaag 8767 accctgtctc aaaaaaaaag aacagattca atgtgccatg
ttgtctgata ttgattcacc 8827 tggggtctaa ccccctacct tcccgcagca
gagcctgctt gtttctattc ttgtcccctg 8887 cccctgccaa ggtggggaag
agggtaggtc cttcaggctc tggtgaatct aatctcaatc 8947 cctccaactt
ctgtgtaagc ctctccagag tctcagtaag tctggaaagc agagatggaa 9007
ttgaggagaa atggaagggg tggagctggt gcctggggtc ctaaaagcct catttgtctc
9067 atctttcctt cta ga tgg aag tga att tgc aca aaa att act gaa gaa
9115 Arg Trp Lys Ile Cys Thr Lys Ile Thr Glu Glu 120 125 att cag
tct att aaa acc atg ggc atg a gaagctgaaa agaatgggat 9163 Ile Gln
Ser Ile Lys Thr Met Gly Met 130 135 cattggactt aaagccttaa
atacccttgt agcccagagc tattaaaacg aaagcatcca 9223 acttgctgtg
tgcctgtgct ctatgggatg ggccctggag gaagtgcagg gagaaaagcc 9283
ctccctggac caacacaagg cataggatgt cctgacccag gcccttggcc agtcacaggc
9343 tgcctggaag gcagagcctc taacaagccc ttttattcac ttggagccac
atccacattg 9403 ctgagcctcc tttgagtcca aatgccactc cagttttcgt
ccccctctta ctcttcacac 9463 attactccta gtgacatttg agcatttcca
aaaattaaat caaattccaa agaaccagga 9523 tttatcatcc tgaaaataat
caaagcctga gccatttata ctaaagccac tttctggtac 9583 ctttatcaga
aattcatctc tcctgccctc tattcgtaca ttctacactg ggccaaagtg 9643
gctggcaatg gctaattagg tcagacagta aagtaatgag ctactacagt gacaactggc
9703 acttggctaa gaagaccaat tgaatccatt aaggttattc ttgtgatgtg
gtgcagagaa 9763 accacttttg actgtgctct agatgtgcaa attatcttcc
ccaaaggact aaagtctctc 9823 aaaggggtct tggtcacctc tttctcctcc
tgcaactttg ttttcctccc ctacagctca 9883 tggctgtgtc ttgcacacac
atgaaccagg gaagatcact catgacttca gggggcaaag 9943 aaagcagtca
gatcttctgc cagacccctc cccaggccag gcacagggtc ttctgctctt 10003
taacatgccc ggagccattg attctagact gttcttccca ccccatctta gtttattttc
10063 tgttgctcat aacagaatat ctaaaactgg ataatttata agacgcaaaa
tgtacttctt 10123 acagttctag agctaggaag tccaaggtca agggggcatg
tctggcaaga gctttcttgc 10183 tggcagggaa tctctgcagg atcccaagct
ggaatagaga atcacatagt gaggtggctg 10243 tccacgctag ctcagctctc
ttcctcttct tagaaagcct ccagtctcac tcctgtggca 10303 aaccattaat
ccattaaccc attaattcat taatccatag atggattaat ctattcacaa 10363
gggcaaagac ctcatgaccc aatcatttct ttttcttttt gttttttaag acagagtcct
10423 gttctgtcgc ccacactgga gttcaacggc tcaatctcgg ctcactgcaa
cctctgcctc 10483 ccgggttcaa gcaattctcc tgcctcagcc tcccaagcag
ctaggattac aggtgcccac 10543 cgccacacct ggctaatttg ttgtattttt
agtagagacg gggtttcacc atgttggcca 10603 ggatggtctc aaactcctga
cctcatatga tccacctgcc taggcctccc aaagtgctga 10663 gattacagac
atgagccact gcgcccagca tgaaccaatc atttcttaat aaccctgcct 10723
ttcaatattg ttactttagg gattaagttt caatgtaagt tttggagggg acaaacactc
10783 aaactatacc attctactcc tggccctctg aaactcatgt ccttctcaaa
tataaatata 10843 ttcattccaa ctccatagcc ccaaaatctt agctcattcc
agcaccaact caaaagtcca 10903 aagtccagag tatcatctgt gagcctgtga
aatacaaacg agttatctac ttttaagata 10963 cagttgtggt aaaagcataa
aacagacatt cccattccaa aatggaggaa tagacaaaaa 11023 gaaacgagta
acaggtctca agcaaatctg aaacccagca gggcagacat taaatcttaa 11083
agctgaagaa taatttcttt tgactctgtg tgtggcctcc catccacaac ggggtatggg
11143 ttaggccccc aagacttcag gcagcctcac ccttatggct ttgctcagtg
cagcccatgt 11203 gactgctcct aggtattgga gtctggtgcc tgaagctttc
ccaggtgggt gttgcatact 11263 gccagtgact gcacacttct gggttcccag
tagtggtccc actcccacag ctctactagg 11323 cattacccta atggagactc
tctacggtgg caccacttcc atggctctgc tagatgggga 11383 ctctttgcag
tggctctgcc cctgtgacaa atctttgcct gggctcctag gcttttgatg 11443
atatcctttg aaatcttggt ggaggctgcc aagctgccac agcttttgct gtctgcaagc
11503 ctgcagagtc agcaccacct ggacactgcc aaggtttatg gcttctacct
tccaaaactg 11563 cagcacaagc tacaattggg gtcacttgag ccttggctag
ggcagccatg aagctctgca 11623 ctggggtttc agggcagagt cccaaggcac
cattctgccc ttctagacct ctgggcctat 11683 aacaggaggg gcaccctcaa
agatctctga aatgcatttc aggtctttct tcatcgtctt 11743 gagaaatagc
atccggctcc cttctatctg tgctaatctt tttagctgca cccttgatct 11803
cctcttctga atgtgctttt tcactcttca tgtggccagg ctgacagttt tccaactctt
11863 tccactctgc ttccagttta atgtaaattt tctttatctt tataattgtc
tttgaattat 11923 tcctttgctc ccaaatctca gcataagtgg ccaaaagtaa
ccatgcacct ccttctatat 11983 tttgcttaga aatttcttct gcagatactc
tagttcgtca ctctcaagtt tggccttcca 12043 caaagccctt aaatgtagac
acagttcagt caagttctct gtcaatttat aacaaggatg 12103 gtctttactc
cagtttccaa taccttattc ctcagttcca tctgaaatct catcagaatg 12163
gccttactgt tcatatttca actagcattc tggtcacaat cacttaacaa atctctaaga
12223 agttccaaac tttccaaaga actgaggtgc tccatgagtt ctccacccct
gcagcaaact 12283 tctgcctgga catctaggtg ttttcataca tcctctgaaa
tctaggtgga ggttcccaaa 12343 ccccaattct t 12354 2 522 DNA Homo
sapiens 2 gaattcgcgg ccgcgcagat tctgtggtta tactcactcc tcatcccaaa
gaatgaaatt 60 taccactctc ctcttcttgg cagctgtagc aggggccctg
gtctatgctg aagatgcctc 120 ctctgactcg acgggtgctg atcctgccca
ggaagctggg acctctaagc ctaatgaaga 180 gatctcaggt ccagcagaac
cagcttcacc cccagagaca accacaacag cccaggagac 240 ttcggcggca
gcagttcagg ggacagccaa ggtcacctca agcaggcagg aactaaaccc 300
cctgaaatcc atagtggaga aaagtatctt actaacagaa caagcccttg caaaagcagg
360 aaaaggaatg cacggaggcg tgccaggtgg aaaacaattc atcgaaaatg
gaagtgaatt 420 tgcacaaaaa ttactgaaga aattcagtct attaaaacca
tgggcatgag aagctgaaaa 480 gaatgggatc attggactta aagccttaaa
tacccttgta gc 522 3 416 DNA Homo sapiens 3 tgaaatttac cactctcctc
ttcttggcag ctgtagcagg ggccctggtc tatgctgaag 60 atgcctcctc
tgactcgacg ggtgctgatc ctgcccagga agctgggacc tctaagccta 120
atgaagagat ctcaggtcca gcagaaccag cttcaccccc agagacaacc acaacagccc
180 aggagacttc ggcggcagca gttcagggga cagccaaggt cacctcaagc
aggcaggaac 240 taaaccccct gaaatccata gtggagaaaa gtatcttact
aacagaacaa gcccttgcaa 300 aagcaggaaa aggaatgcac ggaggcgtgc
caggtggaaa acaattcatc gaaaatggaa 360 gtgaatttgc acaaaaatta
ctgaagaaat tcagtctatt aaaaccatgg gcatga 416 4 138 PRT Homo sapiens
4 Met Lys Phe Thr Thr Leu Leu Phe Leu Ala Ala Val Ala Gly Ala Leu 1
5 10 15 Val Tyr Ala Glu Asp Ala Ser Ser Asp Ser Thr Gly Ala Asp Pro
Ala 20 25 30 Gln Glu Ala Gly Thr Ser Lys Pro Asn Glu Glu Ile Ser
Gly Pro Ala 35 40 45 Glu Pro Ala Ser Pro Pro Glu Thr Thr Thr Thr
Ala Gln Glu Thr Ser 50 55 60 Ala Ala Ala Val Gln Gly Thr Ala Lys
Val Thr Ser Ser Arg Gln Glu
65 70 75 80 Leu Asn Pro Leu Lys Ser Ile Val Glu Lys Ser Ile Leu Leu
Thr Glu 85 90 95 Gln Ala Leu Ala Lys Ala Gly Lys Gly Met His Gly
Gly Val Pro Gly 100 105 110 Gly Lys Gln Phe Ile Glu Asn Gly Ser Glu
Phe Ala Gln Lys Leu Leu 115 120 125 Lys Lys Phe Ser Leu Leu Lys Pro
Trp Ala 130 135 5 5193 DNA Homo sapiens 5 acatttttaa aattttttca
ctcattgctt tgtctttaca cctccccgat ggccaaggtg 60 gaagatcgga
ggcatcacag gagtgtggca gagcttgtgc aggccacagg gcttggcaga 120
gaagacaagc catgtcgagc acagcagcca gggtagaatg gccctcggag atcaacgtgt
180 gcctgtgtct ccaatgcagg agcagtctac cctaaatagt ccatgtcaat
tcctcccttt 240 ggagtctctg cttccccacc agcccccaga acatggccta
acacacaggg aggggaatga 300 ggaaaagaca ttcatcacag ttcagacagg
aagtggtgta tcagtggaga ggtccaagta 360 gaaaacaaat ggcacactca
ggagggctta tatatatata taaatacttt aagttctagt 420 gtacatgtgc
acaatgtgca ggtttgttac atatgtatac atgtgccgtg ttggtttgct 480
gcacccatta actcatcatt taccttaggt atttctccta atgctatccc tcccccatcc
540 ccccacccca caacaggcct cggtgtgtga tgttccccac cctgtgtcca
agtgttgtca 600 ttgttcaatt cccacctatg agtgagaaca tgtggtgttt
ggttttctgt ccttgcgata 660 gtttgctcag aatgatggtt tccagcttta
tccatgtccc tacaaaggac atgaactcaa 720 ccttgtttat ggctgcatag
tattccatgg tgtatatgtg ccacattttc ttaacccagt 780 ctatcattga
tggacatttg ggttggttcc aagtctttac tattgtgaat agtgccacaa 840
taaacataca tgtgcatgca tctttatagt agcatgattt ataatccttt gggtatatac
900 ctagtaatgg gatctttggg ttaaatggta tttctagttc tagatccttg
aggaatcgcc 960 acactgtctt ccacaatggt tgagctagtt tacactccca
ccgatggtgt aaaagcattc 1020 ctatttctcc acatcctctc cagcacctgt
tgtttcctga ctttttaatt attgccattc 1080 taactactgt gagatgatat
ctcattgtgg ttttgatttg catttctctg atggccagtg 1140 atgatgagca
ttttttcatg tgtctgctgg ctgcataaat ctcttctttt caaaagtgtc 1200
tgtccatatc ctttgcccac tttttgatgg ggttgtttga ttttttcttg taaatttgtt
1260 taagttcttt gtagattctg gatattagcc ctttgtcaga tgggtagatt
gcaaaaattt 1320 tctcccattc tgtaggctgc ctgttcactc tgatggtagt
ttcttttgct gtgcagaagc 1380 tctttagttt aattagatcc catttgtcta
ttttggcttt tgttgccatt gcttttggtg 1440 ttttagtcat gaagtacttg
cccatgccta tgtcctgaat ggtattgccc aggttttctt 1500 ctagggtttt
tatggtttta ggtctaacat ttaagtcttt aatctatctt gaattaattt 1560
ttgtataagg tgtaaggaag ggatccagtt tcagctttct acatatggct agccagtttt
1620 cccagcacca tttattaaat aaggaatcct ttccccattt cttgtttttg
tcaggtttgt 1680 caaagatcag atggttgtag atgtgtggtg ttatttctga
ggcctctgtt ctgttccatt 1740 ggtctatatc tgttttggca ccagtaccat
gctgttttgg ttactgtaag ctggtagtat 1800 agtttgaagt caggtaatgt
gatgcctcca gctttgttct ttttgcttag gattgtcttg 1860 gcaatgcagg
cccttttttg gttccatatg aactttaaag tagttttttc cacttctgtg 1920
aagaaagtca ttggtagctt gatggggatg gcattgaatc tctaaattac cttgggcagt
1980 atggccattt tcacgatatt gattcttcct atccaagagc atggaatgtt
cttccatttg 2040 tttgtgtcct cttatttcat tgagcagtgg tttgtggttc
tcaggagggc tttttttaaa 2100 aaaggttctt taaggaaaaa tgtatctatt
atttacagag attcaagtat ggactagcaa 2160 cagcaggaag tctgaagcag
cgtgttgggg ggtgggggtg aataatgtca cctgacaaca 2220 agagatggcg
ctctgaaaca gggaccagac agaagctgca gtcagagaga aacagccatt 2280
gccacaccat gctgcattaa gtatccgtgg ctgtaaaaat tattcaaaca tttggtggct
2340 tcacacatga tttcttatcc acagttgtta tggatcagca gtccaggtac
agatgcgccc 2400 tcagacacag agtctctaat gaggctgcag tcaaggtatc
atcgaggctg cggtcacctc 2460 aaaactcagc tgaggaaaga cccacttcca
agctcagtca cgtggtagct ggcagggttg 2520 agttcctcac agatagctgg
actaagggcc tcggctcctt actggctaca ggctggaggc 2580 tgtcctcggt
ttcttgccac gtaggtctct ccacaggaca gcctcacaca tggccactca 2640
cttcatcaga gcaagctgag aagatccaga gacagagtgt gtgtaaacaa gactaaagtc
2700 atagtctcag aactgactac ctcatcactt tgccatcttc tatttgttag
gagtgaatct 2760 cgagacccag cccagttaag cagaggaaat tacagcaggg
caccaatgcc aagacgtagg 2820 catcaccagg agctatctct gaagtcgtcc
taccacacat gccaaagcaa gagagggagc 2880 aggaggaaca aatgccttga
tctctccctt ttcccaccct cccatctccc acctgtgtct 2940 cccattggcc
aaactcaact cgaagctaga gggcatggga gcccaggtga tacagcccat 3000
agaggcatcc ttccagacca gtgcagagat gagagagaat aaacctgagg cagaggggag
3060 agaggtacaa cgagcagccc atgagacaag acacagatca gcgaactgac
agtcatcatg 3120 gaggtcagat aacctagaaa acaagacatc acacagagca
gcccctcacc catatccatg 3180 aggtccgtca agacattgca cagagcagcc
tctcacccat atccatgagg tctgcctctt 3240 taatctatgc acccaattct
ttcccaggct ctataatttg gggtaacatt tctctgggcc 3300 ctgttataga
ataatgaaaa ttttctataa aacaaatgtc cttactttca cctcatccct 3360
atttatgtaa atgcttgcct ctttaattac tgaggccagg aggaagattt gggagaggaa
3420 agggctatgc ggtgacattt ggaaagaccc tgctctgtga cagttctagt
gttgacgcca 3480 aagttctcat ttcctcttag aaaagtcttg tgcaaatagc
ataatttgct cctgttgact 3540 tttttaatgt gctcatggag actgctcggg
atctagatct gtttgggatc tgcaggactt 3600 ctccttctgc agtgtacaca
cacgtgcaca cacgtatgtg cacataccca gggcactggt 3660 gccatcaaaa
ctttctcttg ttcttcagcc ttccccattc caggtaaggc caccaccacc 3720
ttcaaggctc ccaggcccag accctcaggc aggtagcaat tgccaaggct ttaatgtccc
3780 accccattaa ttttatcttc ctttatctcc tgaaaagatt aatgttctaa
accctggcac 3840 ccaaaacacc ctcatgttga aaactcttca ataccccctt
ctcacactca tcttcagagc 3900 taccatgagg cagagaagcc tccggaatca
gcccacatgg ggctgggtga atgccaacac 3960 caagcaaggg gaaagtcaca
aattgacatc cagcacctta ttctccaccc ttcagcccct 4020 caactgactc
ctgctccacg gcccgttcta ttaatatcta gcatttagca ccagcctgga 4080
caaaaaccta cttggaaaga tggtacaaga accccacaca actccataga acttcgctgt
4140 ctaaaaaaat gctttgccgt atattatccc acttaatctc caccactatg
ctgtacatag 4200 gagccacaac tcctagacaa caaataaaaa tcctatcact
tttcaaatcc taacattttc 4260 atatgacaaa gccagaactc aaaatccaga
cctctagagt cccagatcag gaaaggaaga 4320 aacgccaagt caaagagaag
cttctttaga ataatctgct tttctggatt attcacacca 4380 tgggtcagct
ccccacttga agtcagaacc aagctccaat ttcagtgaac caccatcatg 4440
ctttgaccag gagattctct cagaaatgtg gggtcccatt gagtaggcct gaagacagag
4500 attgacaggc ctatgtgagc ctggaggagt tctttttagg ggctggataa
tgtcaagaac 4560 agagaacaac tccagagaag gcacacacgc cttcaaaccc
atcccctcat ggggagaaag 4620 cagccaggaa ctcaggcctc aagtgttcta
ggtgtggtct cccaaggaaa cgggctcact 4680 tagtttgggg aaaccttcaa
accctgcact gagtcctatg tagactggga cagaaggtgg 4740 acaatgtaat
cccctgagcc ctcaacctcc tcctggagag atgacaagat taagatttct 4800
ctaccagaac cctcaacaga cacatcccag aatctcccca agtgaaatgt gctctaccta
4860 ccgtccctga gagcccaggg gtgtgaaccc agagggcagg tgtggtgggg
aagggaggag 4920 ggagaaagaa aagggatggc tgggagttag agaaaggctc
ctatccagga cctgcctgca 4980 aggatcccag gtatcagcca gcccaaccta
gcccttgttg acttagcagg tgacagtttg 5040 gggaagaagg ggaggaggat
gcggaagtca cacctctcca ggcttggttc ccattggccc 5100 ttgatatcct
taaaagggcc cagcaatttc agcatcctta ttccccagac cttctgcaga 5160
ttctgtggtt atactcactc ctcatcccaa aga 5193 6 2436 DNA Homo sapiens 6
tctcgagacc cagcccagtt aagcagagga aattacagca gggcaccaat gccaagacgt
60 aggcatcacc aggagctatc tctgaagtcg tcctaccaca catgccaaag
caagagaggg 120 agcaggagga acaaatgcct tgatctctcc cttttcccac
cctcccatct cccacctgtg 180 tctcccattg gccaaactca actcgaagct
agagggcatg ggagcccagg tgatacagcc 240 catagaggca tccttccaga
ccagtgcaga gatgagagag aataaacctg aggcagaggg 300 gagagaggta
caacgagcag cccatgagac aagacacaga tcagcgaact gacagtcatc 360
atggaggtca gataacctag aaaacaagac atcacacaga gcagcccctc acccatatcc
420 atgaggtccg tcaagacatt gcacagagca gcctctcacc catatccatg
aggtctgcct 480 ctttaatcta tgcacccaat tctttcccag gctctataat
ttggggtaac atttctctgg 540 gccctgttat agaataatga aaattttcta
taaaacaaat gtccttactt tcacctcatc 600 cctatttatg taaatgcttg
cctctttaat tactgaggcc aggaggaaga tttgggagag 660 gaaagggcta
tgcggtgaca tttggaaaga ccctgctctg tgacagttct agtgttgacg 720
ccaaagttct catttcctct tagaaaagtc ttgtgcaaat agcataattt gctcctgttg
780 acttttttaa tgtgctcatg gagactgctc gggatctaga tctgtttggg
atctgcagga 840 cttctccttc tgcagtgtac acacacgtgc acacacgtat
gtgcacatac ccagggcact 900 ggtgccatca aaactttctc ttgttcttca
gccttcccca ttccaggtaa ggccaccacc 960 accttcaagg ctcccaggcc
cagaccctca ggcaggtagc aattgccaag gctttaatgt 1020 cccaccccat
taattttatc ttcctttatc tcctgaaaag attaatgttc taaaccctgg 1080
cacccaaaac accctcatgt tgaaaactct tcaatacccc cttctcacac tcatcttcag
1140 agctaccatg aggcagagaa gcctccggaa tcagcccaca tggggctggg
tgaatgccaa 1200 caccaagcaa ggggaaagtc acaaattgac atccagcacc
ttattctcca cccttcagcc 1260 cctcaactga ctcctgctcc acggcccgtt
ctattaatat ctagcattta gcaccagcct 1320 ggacaaaaac ctacttggaa
agatggtaca agaaccccac acaactccat agaacttcgc 1380 tgtctaaaaa
aatgctttgc cgtatattat cccacttaat ctccaccact atgctgtaca 1440
taggagccac aactcctaga caacaaataa aaatcctatc acttttcaaa tcctaacatt
1500 ttcatatgac aaagccagaa ctcaaaatcc agacctctag agtcccagat
caggaaagga 1560 agaaacgcca agtcaaagag aagcttcttt agaataatct
gcttttctgg attattcaca 1620 ccatgggtca gctccccact tgaagtcaga
accaagctcc aatttcagtg aaccaccatc 1680 atgctttgac caggagattc
tctcagaaat gtggggtccc attgagtagg cctgaagaca 1740 gagattgaca
ggcctatgtg agcctggagg agttcttttt aggggctgga taatgtcaag 1800
aacagagaac aactccagag aaggcacaca cgccttcaaa cccatcccct catggggaga
1860 aagcagccag gaactcaggc ctcaagtgtt ctaggtgtgg tctcccaagg
aaacgggctc 1920 acttagtttg gggaaacctt caaaccctgc actgagtcct
atgtagactg ggacagaagg 1980 tggacaatgt aatcccctga gccctcaacc
tcctcctgga gagatgacaa gattaagatt 2040 tctctaccag aaccctcaac
agacacatcc cagaatctcc ccaagtgaaa tgtgctctac 2100 ctaccgtccc
tgagagccca ggggtgtgaa cccagagggc aggtgtggtg gggaagggag 2160
gagggagaaa gaaaagggat ggctgggagt tagagaaagg ctcctatcca ggacctgcct
2220 gcaaggatcc caggtatcag ccagcccaac ctagcccttg ttgacttagc
aggtgacagt 2280 ttggggaaga aggggaggag gatgcggaag tcacacctct
ccaggcttgg ttcccattgg 2340 cccttgatat ccttaaaagg gcccagcaat
ttcagcatcc ttattcccca gaccttctgc 2400 agattctgtg gttatactca
ctcctcatcc caaaga 2436 7 1620 DNA Homo sapiens 7 tagatctgtt
tgggatctgc aggacttctc cttctgcagt gtacacacac gtgcacacac 60
gtatgtgcac atacccaggg cactggtgcc atcaaaactt tctcttgttc ttcagccttc
120 cccattccag gtaaggccac caccaccttc aaggctccca ggcccagacc
ctcaggcagg 180 tagcaattgc caaggcttta atgtcccacc ccattaattt
tatcttcctt tatctcctga 240 aaagattaat gttctaaacc ctggcaccca
aaacaccctc atgttgaaaa ctcttcaata 300 cccccttctc acactcatct
tcagagctac catgaggcag agaagcctcc ggaatcagcc 360 cacatggggc
tgggtgaatg ccaacaccaa gcaaggggaa agtcacaaat tgacatccag 420
caccttattc tccacccttc agcccctcaa ctgactcctg ctccacggcc cgttctatta
480 atatctagca tttagcacca gcctggacaa aaacctactt ggaaagatgg
tacaagaacc 540 ccacacaact ccatagaact tcgctgtcta aaaaaatgct
ttgccgtata ttatcccact 600 taatctccac cactatgctg tacataggag
ccacaactcc tagacaacaa ataaaaatcc 660 tatcactttt caaatcctaa
cattttcata tgacaaagcc agaactcaaa atccagacct 720 ctagagtccc
agatcaggaa aggaagaaac gccaagtcaa agagaagctt ctttagaata 780
atctgctttt ctggattatt cacaccatgg gtcagctccc cacttgaagt cagaaccaag
840 ctccaatttc agtgaaccac catcatgctt tgaccaggag attctctcag
aaatgtgggg 900 tcccattgag taggcctgaa gacagagatt gacaggccta
tgtgagcctg gaggagttct 960 ttttaggggc tggataatgt caagaacaga
gaacaactcc agagaaggca cacacgcctt 1020 caaacccatc ccctcatggg
gagaaagcag ccaggaactc aggcctcaag tgttctaggt 1080 gtggtctccc
aaggaaacgg gctcacttag tttggggaaa ccttcaaacc ctgcactgag 1140
tcctatgtag actgggacag aaggtggaca atgtaatccc ctgagccctc aacctcctcc
1200 tggagagatg acaagattaa gatttctcta ccagaaccct caacagacac
atcccagaat 1260 ctccccaagt gaaatgtgct ctacctaccg tccctgagag
cccaggggtg tgaacccaga 1320 gggcaggtgt ggtggggaag ggaggaggga
gaaagaaaag ggatggctgg gagttagaga 1380 aaggctccta tccaggacct
gcctgcaagg atcccaggta tcagccagcc caacctagcc 1440 cttgttgact
tagcaggtga cagtttgggg aagaagggga ggaggatgcg gaagtcacac 1500
ctctccaggc ttggttccca ttggcccttg atatccttaa aagggcccag caatttcagc
1560 atccttattc cccagacctt ctgcagattc tgtggttata ctcactcctc
atcccaaaga 1620 8 862 DNA Homo sapiens 8 aaagagaagc ttctttagaa
taatctgctt ttctggatta ttcacaccat gggtcagctc 60 cccacttgaa
gtcagaacca agctccaatt tcagtgaacc accatcatgc tttgaccagg 120
agattctctc agaaatgtgg ggtcccattg agtaggcctg aagacagaga ttgacaggcc
180 tatgtgagcc tggaggagtt ctttttaggg gctggataat gtcaagaaca
gagaacaact 240 ccagagaagg cacacacgcc ttcaaaccca tcccctcatg
gggagaaagc agccaggaac 300 tcaggcctca agtgttctag gtgtggtctc
ccaaggaaac gggctcactt agtttgggga 360 aaccttcaaa ccctgcactg
agtcctatgt agactgggac agaaggtgga caatgtaatc 420 ccctgagccc
tcaacctcct cctggagaga tgacaagatt aagatttctc taccagaacc 480
ctcaacagac acatcccaga atctccccaa gtgaaatgtg ctctacctac cgtccctgag
540 agcccagggg tgtgaaccca gagggcaggt gtggtgggga agggaggagg
gagaaagaaa 600 agggatggct gggagttaga gaaaggctcc tatccaggac
ctgcctgcaa ggatcccagg 660 tatcagccag cccaacctag cccttgttga
cttagcaggt gacagtttgg ggaagaaggg 720 gaggaggatg cggaagtcac
acctctccag gcttggttcc cattggccct tgatatcctt 780 aaaagggccc
agcaatttca gcatccttat tccccagacc ttctgcagat tctgtggtta 840
tactcactcc tcatcccaaa ga 862 9 19 PRT Homo sapiens 9 Met Lys Phe
Thr Thr Leu Leu Phe Leu Ala Ala Val Ala Gly Ala Leu 1 5 10 15 Val
Tyr Ala 10 119 PRT Homo sapiens 10 Glu Asp Ala Ser Ser Asp Ser Thr
Gly Ala Asp Pro Ala Gln Glu Ala 1 5 10 15 Gly Thr Ser Lys Pro Asn
Glu Glu Ile Ser Gly Pro Ala Glu Pro Ala 20 25 30 Ser Pro Pro Glu
Thr Thr Thr Thr Ala Gln Glu Thr Ser Ala Ala Ala 35 40 45 Val Gln
Gly Thr Ala Lys Val Thr Ser Ser Arg Gln Glu Leu Asn Pro 50 55 60
Leu Lys Ser Ile Val Glu Lys Ser Ile Leu Leu Thr Glu Gln Ala Leu 65
70 75 80 Ala Lys Ala Gly Lys Gly Met His Gly Gly Val Pro Gly Gly
Lys Gln 85 90 95 Phe Ile Glu Asn Gly Ser Glu Phe Ala Gln Lys Leu
Leu Lys Lys Phe 100 105 110 Ser Leu Leu Lys Pro Trp Ala 115 11 23
DNA Artificial Oligonucleotide probe for detecting genomic lacritin
clone 11 agctggggca caggcacccg cac 23 12 23 DNA Artificial
Oligonucleotide probe for detecting genomic lacritin clone 12
ggggttctgg ggctgcagct ggg 23 13 21 DNA Artificial PCR primer
corresponding to nucleotides 523 to 503 of the lacritin gene 13
cgctacaagg gtatttaagg c 21 14 20 DNA Artificial PCR primer for exon
1 of the lacritin gene 14 actcactcct catcccaaag 20 15 19 DNA
Artificial PCR primer for exon 5 of the lacritin gene 15 ttttcagctt
ctcatgccc 19
* * * * *