Epothilone side components
Hoefle, Gerhard ; et al.
Patent Application Summary
U.S. patent application number 10/457098 was filed with the patent office on 2004-03-11 for epothilone side components. This patent application is currently assigned to Gesellschaft Fuer Biotechnologische Forschung, MBH (GBF). Invention is credited to Gerth, Klaus, Hardt, Ingo, Hoefle, Gerhard, Reichenbach, Hans, Sasse, Florenz, Steinmetz, Heinrich.
| Application Number | 20040049051 10/457098 |
| Document ID | / |
| Family ID | 7871162 |
| Filed Date | 2004-03-11 |
| United States Patent Application | 20040049051 |
| Kind Code | A1 |
| Hoefle, Gerhard ; et al. | March 11, 2004 |
Epothilone side components
Abstract
The invention concerns compounds which can be obtained by fermenting DSM 6773, especially epothilones A1, A2, A8, A9, B10, C1, C2, C3, C4, C5, C6, C7, C8, C9, D1, D2, D5, G1, G2, H1, H2, I1, I2, I3, I4, I5, I6 and K and trans-epothilones C1 and C2.
| Inventors: | Hoefle, Gerhard; (Braanschweig, DE) ; Reichenbach, Hans; (Braunschweig, DE) ; Gerth, Klaus; (Braunschweig, DE) ; Hardt, Ingo; (Braunschweig, DE) ; Sasse, Florenz; (Braunschweig, DE) ; Steinmetz, Heinrich; (Braunschweig, DE) |
| Correspondence Address: |
PENNIE AND EDMONDS
1155 AVENUE OF THE AMERICAS
NEW YORK
NY
100362711
|
| Assignee: | Gesellschaft Fuer Biotechnologische
Forschung, MBH (GBF) |
| Family ID: | 7871162 |
| Appl. No.: | 10/457098 |
| Filed: | June 6, 2003 |
Related U.S. Patent Documents
| Application Number | Filing Date | Patent Number | ||
|---|---|---|---|---|
| 10457098 | Jun 6, 2003 | |||
| 09719932 | Mar 21, 2001 | |||
| 6624310 | ||||
| 09719932 | Mar 21, 2001 | |||
| PCT/EP99/04244 | Jun 18, 1999 | |||
| Current U.S. Class: | 548/181 |
| Current CPC Class: | C07D 413/06 20130101; C07D 277/24 20130101; C07D 417/06 20130101; C07D 493/04 20130101 |
| Class at Publication: | 548/181 |
| International Class: | C07D 417/02 |
Foreign Application Data
| Date | Code | Application Number |
|---|---|---|
| Jun 18, 1998 | DE | 19826988.9 |
Claims
What we claim is:
1. Epothilone of formula 14
2. Epothilone of formula 15
3. Epothilone of formula 16
4. Epothilone of formula 17
5. Epothilone of formula 18
6. Epothilone of formula 19
7. Epothilone of formula 20
8. Epothilone of formula 21
9. Epothilone of formula 22
10. Epothilone of formula 23
11. Epothilone of formula 24
12. Compound of formula 25
13. Compound of formula 26
Description
[0001] This application is a division of U.S. application Ser. No. 09/719,932, filed Mar. 21, 2001, allowed, which is the U.S. National Stage of PCT/EP99/04244, filed Jun. 18, 1999, which claims priority to DE 19826988.9, filed Jun. 18, 1998, the disclosure of each of which is incorporated by reference herein in its entirety.
[0002] The invention concerns compounds which in the present context are designated epothilone side components, viz. compounds 5 to 13 and 16 to 39. These compounds can be produced by fermenting DSM 6773 in accordance to DE 41 38 042.8.
[0003] Characterizing data of the compounds according to the invention will be compiled as follows.
[0004] Production: The processing of a raw epothilone mixture, which has been produced by fermenting DSM 6773 in a 900 1 fermentor, can be drawn schematically from FIGS. 1 to 2.
[0005] Activities: cf. table 1 1 2 3 4 5 6 7 8 9 10 11 12 13
[0006] Epothilone A.sub.1 (5): colorless amorphous solid; [.alpha.].sup.22D-69 (c 0.1, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 208 (19600), 247 (13600); IR (KBr) .nu..sub.max 3437, 2959, 2931, 2876, 1732, 1710, 1455, 1259, 978 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.95 (1H, s, H-19), 6.60 (1H, bs, H-17), 5.68 (1H, dd, J=4.4, 4.0 Hz, H-15), 4.12 (1H, m, H-33), 3.71 (1H, m, H-7), 3.52 (1H, bs, 7-OH), 3.37 (1H, bd, J=7.5 Hz, 3-OH), 3.21 (1H, dq, J=7.7, 7.0 Hz, H-4), 3.02 (1H, ddd, J=9.2, 4.5, 2.8 Hz, H-13), 2.87 (1H, ddd, J=8.3, 4.5, 3.7 Hz, H-12), 2.78 (1H, dd, J=16.8, 4.3 Hz, H-2a), 2.70 (3H, s, H-21), 2.66 (1H, dq, J=3.9, 7.0 Hz, H-6), 2.65 (1H, dd, J=16.8, 5.2 Hz, H-2b), 2.16 (1H, ddd, J=15.4, 4.4, 2.8 Hz, H-14 a), 2.12 (3H, bs, H-27), 1.91 (1H, ddd, J=15.4, 9.2, 4.0 Hz, H-14b), 1.63 (1H, m, H-10a), 1.62 (2H, m, H-11), 1.59 (1H, m, H-9a), 1.52 (1H, m, H-10b), 1.39 (1H, m, H-8), 1.35 (1H, m, H-9b), 1.211 (3H, d; J=7.0 Hz, H-2-3), 1.207 (3H, d, J=7.0 Hz, H-24), 0.89 (3H, d, J=6.9 Hz, H-25); EIMS m/z 479 [M].sup.+ (21), 3322 (31), 306 (65), 304 (47), 168 (45), 166 (73), 164 (100), 151 (30), 140 (35); HREIMS m/z 479.2317 (calcd. for C.sub.27H.sub.41NO.sub.5S- , 479.2342).
[0007] Epothilone A.sub.2 (6): colorless amorphous solid; [.alpha.].sup.22D+12.0 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 210 (15100), 248 (15500); IR (KBr) .nu..sub.max 3438, 2963, 2929, 2875, 1734, 1706, 1458, 1262, 981 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.98 (1H, s, H-19), 6.63 (1H, bs, H-17), 5.40 (1H, dd, J=8.3, 3.4 Hz, H-15), 4.26 (1H, ddd, J=8.5, 4.8, 4.7 Hz, H-3), 3.85 (1H, dd, J=7.9, 2.6 Hz, H-7), 3.54 (1H, bs, 3-OH), 3.09 (1H, dq, J=4.8, 7.0 Hz, H-4), 3.01 (1H, ddd, J-8.3, 4.8, 4.6 Hz, H-13), 2.98 (1H, dq, J=7.9, 7.0 Hz, H-6), 2.89 (1H, ddd, J=6.7, 4.6, 4.4 Hz, H12), 2.68 (3H, s, H-21), 2.60 (1H, dd, J=15.1, 8.5 Hz, H-2a), 2.52 (1H, bs, 7-OH), 2.50 (1H, dd, J=15.1, 4.7 Hz, H-2b), 2.18 (1H, ddd, J=15.0, 4.8,3.4 Hz, H-14a), 2.11 (3H, d, J=1.3 Hz, H-27), 1.82 (1H, ddd, J=15.0, 8.3, 8.1 Hz, H-14b), 1.63 (1H, m, H-8), 1.61 (2H, m, H-11a and H-10a), 1.46 (1H, m, H-1 lb), 1.39 (2H, m, H-9), 1.31 (1H, m, H-10b), 1.22 (3H, d, J=7.0 Hz, H-24), 1.15 (3H, d, J=7.0 Hz, H-22), 1.01 (3H, d, J=6.9 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 216.2 (s, C-5), 170.1 (s, C-1), 164.9 (s, C-20), 152.0 (s, C-18), 137.0 (s, C-16), 120.3 (d, C-17), 116.5 (d, C-19), 76.7 (d, C-15), 75.6 (d, C-7), 69.1 (d, C-3), 57.1 (d, C-12), 54.3 (d, C-13), 50.3 (d, C-4), 49.6 (d, C-6), 39.4 (t, C-2), 35.5 (d, C-8), 32.2 (t, C-14), 29.6 (t, C-9), 27.6 (t, C-11), 23.9 (t, C-10), 19.2 (q, C-21), 18.0 (q, C-25), 15.6 (q, C-27), 13.9 (q, C-24), 12.4 (q, C-22); EIMS m/z 479 [M].sup.+ (18), 322 (38), 306 (78), 304 (59), 168 (48), 166 (96), 164 (100), 151 (33), 140 (38); HREIMS m/z 479.2318 (calcd. for C.sub.27H.sub.41NO.sub.5S, 479.2342).
[0008] Epothilone A.sub.8 (7): colorless amorphous solid; [.alpha.].sup.22D-76.2 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (6) 210 (15300), 248 (15500); IR (KBr) .nu..sub.max 3440, 2967, 2932, 2876, 1736, 1691, 1467, 1252, 979 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.95 (1H, s, H-19), 6.64 (1H, dd, J=15.6, 0.9 Hz, H-17), 6.52 (1H, dd, J=15.6, 6.6 Hz, H-16), 5.68 (1H, dddd, J=7.8, 6.6, 3.2, 0.9 Hz, H 15), 4.11 (1H, ddd, J=10.1, 6.6, 3.5 Hz, H-3), 3.78 (1H, ddd, J=5.2, 3.2, 3.2 Hz, H-7), 3.66 (1H, d,.1=6.6 Hz, 3-01-1), 3.23 (1H, dq, 1=5.2, 6.9 Hz,1-1-6), 3.08 (1H, ddd, J=7.3, 5.5,4.1 Hz, H-13), 2.90 (1H, ddd, 0.1=6.6, 4.6, 4.1 Hz, H-12), 2.69 (3H, s, H-21), 2.52 (1H, dd, J=14.7, 10.1 Hz, H-2a), 2.44 (1H, bd, J=3.2 Hz, 7-OH), 2.41 (1H, dd, J=14.7, 3.5 Hz, H-2b), 2.10 (1H, ddd, J=15.0, 5.5, 3.2 Hz, H-14a), 1.90 (1H, ddd, J=15.0, 7.8, 7.3 Hz, H-14b), 1.711 (1H, m, H-8), 1.65 (1H, m, H-11a), 1.50 (1H, m, H-10a), 1.47 (1H, m, H-11b), 1.40(2K m, H-9), 1.39 (1H, m, H-10b), 1.33 (3H, s, H-2-3)), 1.16 (3H, d, J=6.9 Hz, H-24), 1.08 (3H, s, H-22), 0.98 (3H, d, J=7.0 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 75 MHz) .delta. 220.3 (s, C-5), 170.7 (s, C-1), 166.5 (s, C-20), 152.2 (s, C-18), 128.4 (d, C-16), 125.9 (d, C-17), 116.4 (d, C-19), 75.0 (d, C-7), 73.6 (d, C-3), 72.7 (d, C-15), 57.3 (d, C-12), 54.1 (d, C-13), 52.6 (s, C-4), 43.8 (d, C-6), 38.9 (t, C-2), 36.3 (d, C-8), 32.5 (t, C-14), 30.3 (t, C-9), 26.7 (t, C-11), 24.0 (t, C-10), 21.3 (q, C-23), 21.0 (q, C-22), 19.3 (q, C-21), 17.1 (q, C-25), 14.5 (q, C-24); EIMS m/z 479 [M].sup.+ XXX; HRDCIMS m/z 480.2401 (calcd. for C.sub.25H.sub.38NO.sub.6S, 480.2401).
[0009] Epothilone A.sub.9 (8): colorless amorphous solid; [.alpha.].sup.22D-37.6 (c 0.5, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 211 (15500), 253 (14100); IR (KBr) .nu..sub.max 3423, 2965, 2932, 2877, 1736, 1690, 1463, 1249, 1014, 979 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.10 (1H, s, H-19), 6.72 (1H, dd, J=10.7, 4.3 Hz, 27-OH), 6.60 (1H, bs, H-17), 5.69 (1H, dd, J=11.6, 2.0 Hz, H-15), 5.59 (1H, d, J=6.6 Hz, 3-OH), 4.49 (1H, ddd, J=12.9, 4.3, 1.2 Hz, H-27a), 4.27 (1H, ddd, J=11.6, 6.6, 2.9 Hz, H-3), 4.11 (1H, ddd, J=12.9, 10.7, 1.0 Hz, H-27b), 3.71 (1H, ddd, J=4.8, 3.0, 2.8 Hz, H-7), 3.17 (1H, dq, J=3.0, 6.8 Hz, H-6), 3.04 (1H, ddd, J=9.7, 3.6, 2.2 Hz, H-13), 2.93 (1H, bs, 70H), 2.91 (1H, ddd, J=9.7, 3.6, 2.7 Hz, H-12), 2.72 (3H, s, H-21), 2.48 (1H, dd, J=14.2, 11.6 Hz, H-2a), 2.11 (111, dd, J=14.2, 2.9 Hz, H-2b), 2.03 (1H, ddd, J=14.7, 2.2, 2.0 Hz, H-14a), 1.86 (1H, m, H-11a), 1.85 (1H, m, -H-14b), 1.79 (1H, m, H-8), 1.52 (1H, m, H-10a), 1.37 (3H, m, H-9 and H-10b), 1.37 (3H, s, H-23), 1.36 (1H, m, H-11 lb), 1.19 (3H, d, J=6.8 Hz, H-24), 1.02 (3H, d,.1=7.1 Hz, H-25), 1.00 (3H, s, H-22); .sup.13C NMR (CDCl.sub.3, 75 MHz) .delta. 220.5 (s, C-5), 170.2 (s, C-1), 167.5 (s, C-20), 150.7 (s, C-18), 138.9 (s, C-16), 125.2 (d, C-17), 119.5 ((d, C19), 76.7 (d, C-15), 73.4 (d, C-7), 70.4 (d, C-3), 57.7 (d, C-12), 57.2 (t, C-27), 55.3 (d, C-13), 54.2 (s, C-4), 4i:3 (d, C-6), 40.7 (t, C-2), 37.5 (d, C-8), 31.8 (t, C-14), 31.2 (t, C-9), 28.0 (t, C11), 23.7 (q, C-23), 23.2 (t, C-10), 19.2 (q, C-21), 16.8 (q, C-22), 15.8 (q, C-25), 13.5 (q, C-24); EIMS m/z 509 [M].sup.+ (9), 491 (4), 322 (28), 321 (25), 180 (45), 167 (40), 166 (100), 165 (49), 154 (47), 138 (33); HREIMS m/z 509.2467 (calcd. for C.sub.26H.sub.39NO.sub.7S, 509.2447).
[0010] Epothilone B.sub.10 (9): colorless amorphous solid; [.alpha.].sup.22D-27 (c 0.15, MeOH); UV (MeOH) .lambda..sub.max 1M (g) 212 (15800), 247 (12500); IR (KBr) .nu..sub.max 3434, 2962, 2930, 2876, 2858, 1733, 1692, 1461, 1259, 1052, 981 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 600 MHz) .delta. 6.99 (1H, s, H-19), 6.60 (1H, bs, H-17), 5.42 (1H, dd, J=8.0, 3.0 Hz, H-15), 4.25 (1H, ddd, J=9.5, 6.3, 2.8 Hz, H-3), 4.23 (1H, bs, 3-OH), 3.77 (1H, ddd, J=4.0, 3.9, 3.8 Hz, H-7), 3.30 (1H, dq, J=4.0, 6.9 Hz, H6), 3.01 (2H, q, J=7.6 Hz, H-21), 2.81 (1H, dd, J=7.7, 4.6 Hz, H-13), 2.68 (1H, bs, 7-OH), 2.54 (1H, dd, J=13.9, 9.5 Hz, H-2a), 2.36 (1H, dd, J=13.9, 2.8 Hz, H-2b), 2.11 (1H, ddd, J=15.3, 4.6, 3.0 Hz, H-14a), 2.09 (3H, s, H-27), 1.91 (1H, ddd, J=15.3, 8.0, 7.7 Hz, H-14b), 1.74 (1H, m, H-8), 1.73 (1H, m, H-11a), 1.51 (1H, m, H-10a), 1.41 (1H, m, H-11 b), 1.39 (3H, t, J=7.6 Hz, H-28), 1.38 (3H, m, H-9 and H-10b), 1.37 (3H, s, H-23), 1.28 (3H, s, H-26),1.17 (3H, d, J=6.9 Hz, H-24), 1.09 (3H, s, H-22), 1.01 (3H, d, J=7.0 Hz, H-25); EIMS m/z 521 [M].sup.+ (22), 449 (7), 350 (18), 334 (57), 248 (16), 234 (27), 196 (41), 182 (59), 180 (96), 178 (100), 166 (44), 154 (44); HREIMS m/z 521.2808 (calcd. for C.sub.28H.sub.43NO.sub.6S, 521.2811).
[0011] Epothilone G.sub.1 (10): colorless amorphous solid; [.alpha.].sup.22D-39.7 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 203 (15200), 236 (15100); IR(KBr) .nu..sub.max3456, 2962, 2933, 2876, 1736, 1691, 1585, 1466, 1262, 980 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.47 (1H, s, H-19), 6.33 (1H, bs, H-17), 5.42 (1H, dd, J=8.3, 2.9 Hz, H-15), 4.11 (1H, ddd, J=10.1, 6.1, 3.4 Hz, H-3), 3.78 (1H, bddd, J=5.2, 3.5, 3.5 Hz, H-7), 3.63) (1H, bd, J=6.1 Hz, 3-OH), 3.21 (1H, dq, J=5.2, 7.0 Hz, H-6), 3.00 (1H, ddd, J=7.7, 4.8, 4.2 Hz, H-13), 2.88 (1H, ddd, J=7.1, 4.2, 4.2 Hz, H12), 2.53 (1H, dd, J=14.8, 10.1 Hz, H-2a), 2.51 (1H, bd, J=3.5 Hz, 7-OH), 2.43 (1H, dd, J=14.8, 3.4 Hz, H-2b), 2.43 (3H, s, H-21), 2.07 (1H, ddd, J=15.1, 4.8, 2.9 Hz, H-14a), 1.99 (3H, d, J=1.3 Hz, H-27), 1.86 (1H, ddd, J=15.1, 8.3, 7.7 Hz, H-14b), 1.71 (1H, m, H-8), 1.69 (1H, m, H-1 la), 1.53 (1H, m, H-10a), 1.42 (1H, m, H-1 ib), 1.40 (3H, m, H-9 and H-10b), 1.34 (3H, s, H-23), 1.16 (3H, d, J=7.0 Hz, H-24), 1.09 (3H, s, H-22), 0.99 (3H, d, J=6.9 Hz, H25); .sup.13C NMR, (CDCl.sub.3, 100 MHz) .delta. 220.1 (s, C-5), 170.5 (s, C-1), 161.0 (s, C-20), 1374 (s, C18), 136.7 (s, C-16), 135.9 (d, C-19), 116.4 (d, C-17), 76.4 (d, C-15), 74.9 (d, C-7), 73.7 (d, C3), 57.4 (d, C-12), 54.4 (d, C-13), 52.6 (s, C-4), 43.8 (d, C-6), 38.8 (t, C-2),") 6.2 (d, C-8), 31.4 (t, C-14), 30.4 (t, C-9), 27.0 (t, C-11), 23.9 (t, C-10), 21.3 (q, C-23), 21.2 (q, C-22), 17.2 (q, C25), 15.8 (q, C-27), 14.4 (q, C-24), 13.8 (q, C-21); EIMS m/z 477 [M].sup.+ (4), 405 (7), 290 (40), 152 (39), 150 (100), 14S (23), 124 (23); HREIMS m/z 477.2684 (calcd. for C.sub.26H.sub.39NO.sub.7, 477.2727).
[0012] Epothilone G.sub.2 (11): colorless amorphous solid; [.alpha.].sup.22D-22.6 (c 1.0, MeOH); W (MeOH) .lambda..sub.max nm (.epsilon.) 202 (21500), 236 (14800); IR (KBr) .nu..sub.max3456, 2965, 2934, 2877, 1737, 1690, 1586, 1464, 1250, 980 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.48 (1H, s, H-19), 6.33 (1H, bs, H-17), 5.43 (IF, dd, J=7.1, 3.6 Hz, H-15), 4.12 (1H, ddd, J=9.9, 6.4, 3.4 Hz, H-3), 3.77 (1H, ddd, J=4.7, 4.4, 4.1 Hz, H-7), 3.83 (1H, bd, J=6.4 Hz, 3-OH), 3.30 (1H, dq, J=4.7, 6.9 Hz, H-6), 2.78 (1H, dd, J=7.0, 5.4 Hz, H-13), 2.54 (1H, dd, J=14.3, 9.9 Hz, H-2a), 2.51 (1H, bd, J=4.1 Hz, 7-OH), 2.44 (3H, s, H-21), 2.40 (1H, dd, J=14.3, 3.4 Hz, H-2b), 2.03 (1H, ddd, J=15.2, 5.4, 3.611z, H-14a), 2.00 (3H, d, J=1.3 Hz, H-27), 1.92 (1H, ddd, J=15.1, 7.1, 7.0 Hz, H-14b), 1.71 (1H, m, H-8), 1.68 (1H, m, H-11a), 1.51 (1H, m, H-10a), 1.42 (1H, M, H-11b), 1.39 (3H, m, H-9 and H-10b), 1.35 (3H, s, H-23), 1.26 (3H, s, H-26), 1.16 (3H, d, J=6.9 Hz, H-24), 1.07 (3H, s, H-22), 0.99 (3H, d, J=7.0 Hz, H-25); .sup.13CNMR (CDCl.sub.3, 100 MHz) .delta. 220.7 (s, C-5), 170.5 (s, C-1), 161.0 (s, C-20), 137.4 (s, C-18), 136.5 (s, C-16), 135.9 (d, C-19), 116.3 (d, C-17), 76.6 (d, C-15), 74.6 (d, C-7), 73.5 (d, C-3), 61.3 (s, C-12), 61.1 (d, C-13), 52.7 (s, C4), 43.4 (d, C-6), 39.0 (t, C-2), 36.5 (d, C-8), 32.0 (t, C-1), 31.8 (t, C-14), 30.8 (t, C-9), 22.8 (t, C-10), 22.9 (q, C-26), 21.0 (q, C-23), 20.8 (q, C-22), 17.2 (q, C-25), 15.9 (q, C-27), 14.1 (q, C-24), 13.8 (q, C-21); EIMS m/z 491 [M].sup.+ (21), 419 (6), 320 (18), 304 (39), 166 (42), 152 (57), 150 (100), 149 (44), 148 (58), 124 (35), 109 (33); HREIMS m/z 491.2878 (calcd. for C.sub.27H.sub.41NO.sub.7, 491.2883).
[0013] Epothilone H.sub.1 (12): colorless amorphous solid; [.alpha.].sup.22D-84.2 (c 0.2, MeOH); WV (MeOH) .lambda..sub.max nm (.epsilon.) 203 (19600), 237 (12000); IR (KBr) .nu..sub.max3436, 2933, 2880, 2860, 1734, 1688, 1585, 1251, 1007 cm.sup.-1; .sup.1H NMR(CDCl.sub.3, 400 MHz) .delta. 7.47 (1H, s, H-19), 6.31 (1H, bs, H-17), 5.43 (1H, ddd, J=10.6, 10.2, 4.5 Hz, H-12), 5.36 (1H, dddd, J=10.6, 9.6, 5.0, 1.3 Hz, H-13), 5.30 (1H, dd, J=9.9, 2.0 Hz, H-15), 4.16 (1H, ddd, J=11.2, 5.3, 2.8 Hz, H-3), 3.73 (1H, ddd, .I=3.9, 2.5, 2.3 Hz, H-7), 3.12 (1H, dq, J=2.3, 6.9 Hz, H-6), 2.92 (1H, d, J=2.5 Hz, 7-OH), 2.91 (1H, d, J=5.3 Hz, 7-OH), 2.66 (1H, ddd, J=15.1, 9.9, 9.6 Hz, H-14a), 2.50 (1H, dd, J=15.4, 11.2 Hz, H-2a), 2.43 (3H, s, H-21), 2.37 (1H, dd, J=15.4, 2.8 Hz, H-2b), 2.23 (1H, m, H-14b), 2.18 (1H, m, H-11a), 2.01 (1H, m, H-11b), 2.08 (3H, d, J=1.3 Hz, H-27), 1.74 (1H, m, H-8), 1.65 (1H, m, H-10a ), 1.33 (1H, m, H-9a), 1.31 (3H, s, H-23), 1.19 (1H, m, H-10b), 1.18 (1H, m, H-9b), 1.17 (3H, d, J=6.9 Hz, H-24), 1.08 (3H, s, H-22), 0.99 (3H, d, J=7.1 Hz, H-25);.sup.13C NMR, see Table 1; EMS m/z 461 [M].sup.+ (6), 310 (5), 274 (10), 273 (7), 171 (63), 152 (100), 148 (18), 111 (15); HREIMS m/z 461.2743 (calcd. for C.sub.26H.sub.39NO.sub.6, 461.2777).
[0014] Epothilone H.sub.2 (13): colorless amorphous solid; [.alpha.].sup.22D -44.4 (c 0.25, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 203 (14500), 236 (12200); IR (KBr) .nu..sub.max. 3436, 2967, 2935, 2880, 1734, 1690, 1586, 1251, 1007 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.46 (1H, s, H-19), 6.30 (1H, bs, H-17), 5.23 (1H, dd, J=9.8, 2.1 Hz, H-15), 5.12 (1H, dd, J=10.1, 5.3 Hz, H-13), 4.20 (1H, ddd, J=10.8, 5.7, 2.9 Hz, H-3), 3.71 (1H, ddd, J=3.8, 2.6, 2.6 Hz, H-7), 3.14 (1H, dq, J=2.6, 6.9 Hz, H-6), 2.93 (d, J=5.7 Hz, 3-OH), 2.90 (1H, bd, J=2.6 Hz, 7-OH), 2.62 (1H, ddd, J=15.1, 9.8, 9.8 Hz, H-14a), 2.46 (1H, dd, J=15.1, 10.8 Hz, H-2a), 2.43 (3H, s, H-21), 2.32 (1H, dd, J=15.1, 2.9 Hz, H-2b), 2.29 (1H, m, H-11a), 2.19 (1H, bd, J=15.1 Hz, H-14b), 1.97 (3H, d, J=1.3 Hz, H-27), 1.87 (1H, m, H-1 lb), 1.73 (1H, m, H-8), 1.67 (1H, m, H-10a ), 1.65 (3H, bs, H-26), 1.32 (3H, s, H-23), 1.26 (2H, m, H-9), 1.24 (1H, m, H-10b), 1.18 (3H, d, J=6.9 Hz, H-24), 1.07 (3H, s, H-22), 1.00 (3H, d, J=7.0 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 220.6 (s, C-5), 170.3 (s, C-1), 161.0 (s, C-20), 138.6 (s, C-12), 138.4 (s, C-16), 137.5 (s, C-18), 135.6 (d, C-19), 120.8 (d, C-13), 115.8 (d, C-17), 78.9 (d, C-15), 74.3 (d, C-7), 72.7 (d, C-3), 53.3 (s, C-4), 42.0 (d, C-6), 39.6 (t, C-2), 38.6 (d, C-8), 32.4 (t. C-14), 31.9 (t, C-9), 31.6 (t, C-Il), 25.6 (t, C-10), 23.0 (q, C-26), 22.8 (q, C-23), 18.8 (q, C-22), 16.1 (q, C-27), 15.9 (q, C-25), 13.8 (q, C-21), 13.6 (q, C-24); EIMS m/z 475 [M].sup.+ (11), 288 (9), 287 (5), 188(7), 171 (32), 152 (100), 111 (10); HREIMS m/z 475.2913 (calcd. for C.sub.27H.sub.41NO.sub.6, 475.2934).
[0015] Epothilone C.sub.1 (16): colorless amorphous solid; [.alpha.].sup.22D -114.0 (c 10.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 211 (16500), 248 (12500); IR (KBr) .nu..sub.max3440, 2933, 2877, 2858, 1730, 1708, 1457, 1244, 981 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.96 (1H, s, H-19), 6.56 (1H, bs, H-17), 5.47 (1H, dd, J=9.2, 3.0 Hz, H-15), 5.43 (1H, m, H-12), 5.40 (1H, m, H-13), 4.40 (1H, ddd, J=6.2, 6.1, 6.1 Hz, H-3), 3.69 (1H, dd, J=5.7, 3.6 Hz, H-7), 3.01 (1H, dq, J=5.7, 6.9 Hz, H-6), 3.01 (1H, bs, 3-OH), 2.84 (1H, dq, J=5.2, 7.0 Hz, H-4), 2.68 (3H, s, H-21), 2.66 (1H, ddd,.1=16.4, 9.2, 7.3 Hz, H-14a), 2.64 (1H, dd, J=15.9, 7.1 Hz, H-2a), 2.54 (1H, dd, J=15.9, 6.1 Hz, H-2b), 2.38 (1H, bd, J=16.4 Hz, H-14b), 2.35 (1H, bs, 7-OH), 2.07 (3H, bs, H-27), 2.03 (2H, m, H-i 1), 1.62 (1H, m, H-10a), 1.53 (1H, m, H-8), 1.35 (1H, m, H-9a), 1.22 (1H, M, H-9b), 1.19 (3H, d, J=6.9 Hz, H-24), 1.14 (3H, d, J=6.9 Hz, H-23), 1.10 (1H, m, H-10b), 0.95 (3H, d, J=6.9 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (5), 324 (8), 290 (8), 204 (7), 168 (100), 164 (15), 139 (36); HREIMS m/z 463.2381 (calcd. for C.sub.23H.sub.37NO.sub.5S, 463.2392).
[0016] Epothilone D.sub.1 (17): colorless amorphous solid; [.alpha.].sup.22D -118.6 (c 0.5, MeOH); UV (MeOH) .lambda..sub.max n-m (.epsilon.) 208 (18300), 249 (11900); IR (KBr) .nu..sub.max3439, 2965, 2934, 2877, 1729, 1707, 1456, 1250, 980 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.98 (1H, s, H-19), 6.56 (1H, bs, H-17), 5.51 (1H, dd, J=9.5, 3.4 Hz, H-15), 5.16 (1H, dd, J=8.0, 4.2 Hz, H-13), 4.42 (1H, ddd, J=7.1, 6.3, 5.5 Hz, H-3), 3.70 (1H, dd, J=6.5, 2.9 Hz, H-7), 3.07 (1H, dq, J=6.5, 6.9 Hz, H-6), 2.95 (1H, dq. J=4.7, 7.0 Hz, H-4), 2.71 (3H, s, H-21), 2.69 (1H, dd, J=16.0, 6.3 Hz, H-2a), 2.64 (1H, m, H-14a), 2.59 (1H, dd, J=16.0, 7.1 Hz, H-2b), 2.46 (1H, bs, 3-OH), 2.38 (1H, bd, J=16.0 Hz, H-14b), 2.19 (1H, ddd, J=13.3,8.6,5.7 Hz,H-11a), 2.10(3H, d, J=1.4 Hz, H-27), 2.02 (1H, bs, 7-OH), 1.91 (1H, ddd, J=13.3, 6.0, 6.0 Hz, H-1 lb), 1.68 (1H, m, H-10a ), 1.66 (3H, bs, H-26), 1.53 (1H, m, H-8), 1.37 (1H, m, H-9a), 1.26 (1H, m, H-9b), 1.24 (3H, d, J=6.9 Hz, H-24), 1.19 (1H, m, H-10b), 1.14 (3H, d. J=7.0, H-23), 0.99 (3H, d, J=6.9 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 217.0 (s, C-5), 169.7 (s, C-1), 165.0 (s, C-20), 152.2 (s, C18), 1-38.5 (s, C-12), 137.7 (s, C-16), 120.7 (d, C-13), 120.1 (d, C-17), 116.3 (d, C-19), 78.8 (d, C-15), 77.2 (d, C-7), 67.7 (d, C-3), 52.1 (d, C-4), 46.5 (d, C-6), 40.6 (t, C-2), 37.6 (d, C-8), 32.3 (t, C-14), 31.8 (t, C-11), 29.5(t, C-9), 25.5 (t, C-10), 23.1 (q, C-26), 19.2 (q, C-21), 15.5 (q, C27), 16.6 (q, C-25), 14.5 (q, C-24), 9.7 (q, C-23); EIMS m/z 477 [M].sup.+ (13), 304 (19), 303 (31), 218 (40), 204 (41), 168 (100), 164 (45), 157 (25), 139 (18); HREIMS m/z 477.2544 (calcd. for C.sub.26H.sub.39NO.sub.5S, 477.2549).
[0017] Epothilone C.sub.2 (18): colorless amorphous solid; [.alpha.].sup.22D -11.6 (c 10.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 212 (15500), 249 (12100); IR (KBr) .nu..sub.max3428, 2962, 2929, 2877, 2859, 1734, 1705, 1460, 1251, 9S2 cm.sup.-1;.sup.1H NM (CDCl.sub.3, 300 MHz) .delta. 6.99 (1H, s, H-19), 6.66 (1H, bs, H-17), 5.55 (1H, ddd, J=10.4, 9.2, 6.1 Hz, H-12), 5.38 (1H, ddd, J=10.4, 9.3, 6.2 147, H-13), 5.22 (1H, dd, J=8.8, 2.8 Hz, H-15), 4.42 (1H, dddd, J=9.4, 5.6, 4.2, 4.1 Hz, H-3), 3.93 (1H, d, J=5.6 Hz, 3-OH), 3.86 (1H, m, H-7), 3.15 (1H, bs, 7-OH), 3.12 (1H, dq, J=4.2, 7.0 Hz, H-4), 3.00 (1H, dq, J=6.9, 7.0 Hz, H-6), 2.70 (3H, s, H-21), 2.62 (1H, dddd, J=15.1, 9.3, 8.8, 0.8 Hz, H-14a), 2.58 (1H, dd, J=15.4, 9.4 Hz, H-2a), 2.38 (1H, dd, J=15.4, 4.1 Hz, H-2b), 2.31 (1H, ddd,.1=15.1, 6.2, 2.8 Hz, H-14b), 2.08 (3H, d, J=1.3 Hz, H-27), 2.15 (1H, m, H-11a), 2.04 (1H, m, H-11b), 1.71 (1H, m, H-8), 1.59 (1H, m, H-10a), 1.43 (1H, m, H-9a), 1.31 (114, m, H-9b), 1.26 (3H, d, J=7.0 Hz, H-24), 1.15 (3H, d, J=7.0 Hz, H-23), 1.11 (1H, m, H-10b), 1.00 (3H, d, J=6.9 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (7), 324 (7), 306 (8), 290 (17), 168 (100), 164 (14), 139 (27); HREIMS m/z 463.2392 (calcd. for C.sub.25H.sub.37NO.sub.5S- , 463.2392).
[0018] Epothilone D.sub.2 (19): colorless amorphous solid; [.alpha.].sup.22D -12.5 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 210 (15400), 248 (11200); IR (KBr) .nu..sub.max 3436, 2965, 2930, 2877, 1732, 1705, 1458, 1253, 980 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.97 (1H, s, H-19), 6.56 (1H, bs, H-17), 5.18 (IF, dd, J=7.9, 4.9 Hz, H-15), 5.18 (1H, ddd, J=9.6, 5.4, 1.0 Hz, H-13), 4.27 (1H, m, H-3), 3.88 (1H, dd, J=5.6, 4.6 Hz, H-7), 3.19 (1H, bs, 3-OH), 3.07 (1H, dq, J=4.3, 7.0 Hz, H-4), 2.95 (1H, dq, J=5.6, 7.0 Hz, H-6), 2.70 (3H, s, H-21), 2.62 (1H, dd, J=14.9, 7.8 Hz, H-2a), 2.56 (1H, ddd, J=14.7, 9.6, 7.9 Hz, H-14a), 2.43 (1H, dd, J=14.9, 5.6 Hz, H-2b), 2.38 (1H, bs, 7-OH), 2.26 (1H, ddd, J=14.5, 5.4, 4.9 Hz, H-14b), 2.19 (1H, ddd, J=13.0, 10.4, 5.4 Hz, H-11a), 2.10 (3H, d, 0.1=1.4 Hz, H-27), 1.95 (1H, ddd, J=13.0, 10.3, 5.3 Hz, H-11b), 1.72 (1H, m, H--S), 1.68 (3H, bs, H-26), 1.61 (1H, m, H-10a ), 1.39 (2H, m, H-9), 1.21 (1H, m, H-10b), 1.19 (3H, d, J=6.9 Hz, H-24),1.17 (3H, d. J=7.0, H-22),1.00 (3H, d, J=6.9 Hz, H-25J; .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 216.8 (s, C-5), 170.4 (s, C-1), 164.9 (s, C-20), 152.3 (s, C-18), 139.8 (s, C-12), 137.5 (s, C-16), 120.5 (d, C-17), 119.2 (d, C-13), 116.3 (d, C-19), 80.0 (d, C-15), 74.3 (d, C-7), 69.7 (d, C-3), 48.6 (d, C-4), 48.4 (d, C-6), 39.9 (t, C-2), 36.6 (d, C-8), 32.2 (t, C-14), 32.7 (t, C-11), 30.9 (t, C-9), 26.0 (t, C-10), 23.6 (q, C-26), 19.2 (q, C-21), 15.4 (q, C-27), 17.1 (q, C-25), 12.4 (q, C-24), 12.7 (q, C-23); EIMS m/z 477 [M].sup.+ (22), 304 (19), 303 (17), 218 (22), 204 (25), 163 (100), 164 (28), 157 (31), 139 (21); HREIMS m/z 477.2545 (calcd. for C.sub.26H.sub.39NO.sub.5S, 477.2549).
[0019] Epothilone C.sub.3 (20): colorless amorphous solid; [.alpha.].sup.22D -62.1 (c 5.0, MeOH); UV (MeOH)).lambda..sup.max nm (.epsilon.) 212 (16200), 248 (12300); IR (KBr) .nu..sub.max 3432, 2928, 2878, 2858, 1736, 1698, 1252, 1040 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.95 (1H, s, H-19), 6.56 (1H, bs, H-17), 5.44 (1H, ddd, J=10.9, 10.3, 5.4 Hz, H-12), 5.33 (1H, ddd, J=10.9, 9.3, 4.6 Hz, H-13), 5.23 (1H, dd, J=9.5, 2.2 Hz, H-15), 4.36 (1H, ddd, J=11.3, 5.6, 2.3 Hz, H-3), 4.04 (1H, d, J=5.6 Hz, 3-OH), 3.93 (1H, ddd,.1=9.5, 2.3, 1.4 Hz, H-7), 3.56 (1H, bd, J=2.3 Hz, 7-OH), 2.70 (1H, dd, J=18.0, 1.4 Hz, H-6a), 2.67 (3H, s, H-21), 2.61 (1H, ddd, J=15.3, 9.5, 9.3 Hz, H-14a), 2.38 (1H, dd,.1=14.3, 11.3 Hz, H-2a), 2.36 (1H, dd, J=18.0, 9.5 Hz, H-6b), 2.28 (1H, bd, J=15.3 Hz, H-14b), 2.12 (1H, m, H-11a), 2.06 (1H, dd, J=14.3, 2.3 Hz, H-2b), 2.03 (3H, d, J=1.3 Hz, H-27),1.96 (1H, m, H-1 lb), 1.75 (1H, m, H-8), 1.54 (1H, m, H-10a), 1.26 (1H, m, H-9a), 1.25.(3H, s, H-23), 1.17 (1H, m, H-10b), 1.15 (1H, m, H-9b), 1.03 (3H, s, H-22), 0.91 (3H, d. J=6.8 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (28), 290 (14), 168 (100), 164 (36), 157 (44). 151 (25), HREIMS m/z 463.2379 (calcd. for C.sub.25H.sub.37NO.sub.5S, 463.2392).
[0020] Epothilone C.sub.4 (21): colorless amorphous solid; [.alpha.].sup.22D -75.6 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 212 (17200), 248 (12500); IR (KBr) .nu..sub.max 3434, 2974, 2932, 2859, 1735, 1686, 1252, 1046 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.96 (1H, s, H-19), 6.60 (1H, bs, H-17), 5.43 (1H, m, H-12), 5.40 (1H, m, H-13), 5.26 (1H, dd, J=9.6, 2.3 Hz, H-15), 4.41 (1H, ddd, J=11.4, 5.8,2.5 Hz, H-3), 3.78 (1H, m, H-7), 3.70 (1H, bs, 3-OH), 3.46 (1H, d, J=0.9 Hz, 7-OH), 3.01 (1H, dq, J=0.5, 7.0 Hz, H-6), 2.69 (3H, s, H-21), 2.66 (1H, ddd, J=15.3, 9.6, 8.8 Hz, H-14a), 2.47 (1H, dd, J=14.5, 11.4 Hz, H-2a), 2.29 (1H, m, H-1.4b), 2.25 (1H, dd, J=14.5, 2.5 Hz, H-2b), 2.24 (1H, m, H-11a), 2.07 (3H, d, J=1.4 Hz, H-27), 1.96 (1H, m, H-11b), 1.51 (2H, m, H-8), 1.44 (2H, m, H-10), 1.37 (2H, m, H-9), 1.32 (3H, s, H-23), 1.17 (3H, d, J=7.0 Hz, H-24), 1.07 (3H, s, H-22); .sup.13C NMR , see Table 1; EIMS m/z 463 [M].sup.+ (7), 276 (15), 171 (33), 168 (100), 164 (23), 151 (22), 111 (13); HREIMS m/z 463.2373 (calcd. for C.sub.25H.sub.37NO.sub.5S, 463.2392).
[0021] Epothilone C.sub.5 (22): colorless amorphous solid; [.alpha.].sup.22D -158.2 (c 0.5, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 205 (19500), 247 (12700); IR (KBr) .nu..sub.max. 3447, 2972, 2927, 1737, 1690, 1450,1252,1181, 936 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.93 (1H, s, H-19), 6.48 (1H, bs, H-17), 5.48 (1H, ddd,. J=10.7, 6.2, 6.2 Hz, H-12), 5.39 (1H, m, H-13), 5.37 (1H, M, H-9), 5.34 (1H, dd, J=8.0, 2.3 Hz, H-15), 4.29 (1H, dd, J=6.0, 2.6 Hz, H-7), 4.09 (1H, ddd, J=10.8, 7.1, 2.9 Hz, H-3), 3.59 (1H, d, J.=7.1 Hz, 3-OH), 3.17 (1H, dq, J=6.0, 6.9 Hz, H-6), 2.68 (3H, s, H-21).2.54 (1H, ddd, J=15.2, 8.1, 8.0 Hz, H-14a), 2.44 (1H, bs, 7-OH), 2.42 (1H, dd, J=15.1, 2.9 Hz, H-2a), 2.41 (1H, ddd, J=15.2, 2.3, 2.3 Hz, H-14b), 2.34 (1H, dd, J=15.1, 10.8 Hz, H2b), 2.20 (1H, m, H-10a ), 2.18 (2H, m, H-11), 2.12 (1H, m, H-10b), 2.06 (3H, bs, H-27), 1.67 13 (3H, bs, H-25), 1.27 (3H, s, H-23), 1.21 (3H, d, J=6.9 Hz, H-24), 1.15 (311, s, H-22); .sup.13C NMR, see Table 1; EIMS m/z 475 [M].sup.+ (6), 392 (7), 304 (6), 288 (33), 204 (76), 171 (19), 168 (100), 164 (12); REIMS m/z 475.2380 (calcd. for C.sub.26H.sub.37NO.sub.5S, 475.2392).
[0022] Epothilone D.sub.5 (23): colorless amorphous solid; [.alpha.].sup.22D -150 (c 0.2, MeOH); UV (MeOH)) .lambda..sub.max nm (.epsilon.) 205 (23300), 248 (13600); IR (KBr) .nu..sub.max 3439, 2967, 2927, 1736, 1690, 1451, 1254, 1181, 987 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) 5; 6.94 (1H, s, H-19), 6.51 (1H, bs, H-17), 5.34 (1H, bs, H-9), 5.29 (1H, dd, J=8.0, 2.4 Hz, H-15), 5.16 (1H, dd, J=8.2, 6.2 Hz, H-13), 4.30 (1H, bd, J=4.9 Hz, H-7), 4.19 (1H, ddd, J=10.8, 7.6, 3.0 Hz, H-3), 3.68 (1H, d, J=7.6 Hz, 3-OH), 3.17 (1H, dq, J=4.9, 7.0 Hz, H-6), 2.69 (3H, s, H-21), 2.65 (1H, d, J=2.1 Hz, 7-OH), 2.56 (1H, ddd, J=16.2, 8.2, 8.0 Hz, H-14a), 2.40 (1H, dd, J=15.0, 3.0 Hz, H-2a), 2.39 (1H, bd, J=16.2 Hz, H-14b), 2.34 (1H, dd, J=15.0, 10.8 Hz, H-2b), 2.25 (2H, m, H-10a and H-11a), 2.20(1H, m, H-10b), 2.17 (1H, m, H-11b), 2.05 (3H, d, J=1.0 Hz, H-27), 1.69 (3H, bs, H-25), 1.68 (3H, bs, H-26), 1.29 (3H, s, H-23), 1.23 (3H, d, J=7.0 Hz, H-24), 1.16 (3H, s, H-22); .sup.13C NMR, see Table 1; EIMS m/z 489 [M].sup.+ (4), 406 (4), 338 (7), 302 (13), 218 (35), 171 (10), 168 (100), 153 (20), 125 (10); HREIMS m/z 489.2536 (calcd. for C.sub.27H.sub.39NO.sub.5S, 489.2549).
[0023] Epothilone C.sub.6 (24): colorless amorphous solid; [.alpha.]22 D -205.2 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 218 (24600), 237 (28800); IR (KBr) .nu..sub.max 3435, 2967, 2927, 2882, 1732, 1688, 1465, 1258, 988 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.97 (1H, s, H-19), 6.58 (1H, bs, H-17), 6.43 (1H, dd, 15.5, 10.8 Hz, H-11), 6.11 (1H, dd, J=10.8, 10.6 Hz, H-12), 5.75 (1H, ddd, J.=15.5,8.3,5.6 Hz, H-10), 5.34 (1H, m, H-13), 5.34 (1H, dd, J=9.7, 2.4 Hz, H-15), 4.16 (1H, ddd, .J=9.2, 4.9, 4.3 Hz, H-33), 3.74 (1H, ddd, J=2.2, 2.1, 1.7 Hz, H-7), 3.24 (1H, dq, J=2.1, 6.9 Hz, H-6), 3.06 (1H, d, J=2.2 Hz, 7-OH), 2.93 (1H, d, J=4.9 Hz, 3-OH), 2.78 (1H, dddd, J=14.1, 9.9 9.7, 0.7, H-14a), 2.71 (3H, s, H-21), 2.48 (1H, m, H-9a), 2.47 (1H, dd, J=15.5, 9.2 Hz, H-2a), 2.40 (1H, dd, J=15.5, 4.3 Hz, H-2b), 2.38 (1H, bdd, J=14.1, 7.8 Hz, H-14b), 2.11 (3H, d, J=1.3 Hz, H-27), 1.96 (1H, m, H-8), 1.33 (3H, s, H-23), 1.11 (3H, d, J=6.9 Hz, H-24), 1.06 (3H, s, H-22), 1.05 (3H, d, J=6.8 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 475 [M].sup.+ (13), 387 (2), 316 (4), 288 (15), 230 (16), 204 (9), 171 (18), 168 (100), 164 (14), 151 (17); HREIMS m/z 475.2361 (calcd. for C.sub.26H.sub.37NO.sub.5S, 475.2392).
[0024] Epothilone C.sub.7 (25): colorless amorphous solid; [C].sup.22D -XXX (c 2.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX (XXX), XXX (XXX); IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.01 (1H, s, H-19), 6.66 (1H, bs, H-17), 5.59 (1H, ddd, J=11.1, 11.1, 3.8 Hz, H-12), 5.40 (1H, dd, J=11.1, 9.2, H-13), 5.03 (1H, d, J=9.3 Hz, H-15), 4.62 (1H, dd, J=9.3, 9.2 Hz, H-14), 4.18 (1H, bd, J=11.0 Hz, H-3), 3.72 (1H, bs, H-7), 3.20 (1H, bs, 3-OH), 3.09 (1H, dq, J=1.9, 6.8 Hz, H-6), 3.00 (1H, bs, 7-OH), 2.69 (3H, s, H-21), 2.47 (1H, dd, J=14.8, 11.0 Hz, H-2a), 2.32 (1H, dd, J=14.8, 2.6 Hz, H-2b), 2.27 (1H, m, H-11a), 2.19 (3H, bs, H-27), 2.13 (1H, m, H-11b), 1.76 (1H, m, H-8), 1.70 (1H, m, H-10a ), 1.35 (1H, m, H-9a), 1.32 (3H, s, H-23), 1.23 (1H, m, H-9b), 1.21 (1H, m, H-10b), 1.18 (3H, d, J=6.8 Hz, H-24), 1.08 (3H, s, H-22), 1.00 (3H, d,.]=6.9 Hz, H-25); EIMS m/z 493 [M].sup.+XXX; HREIMS m/z 493.XXX (calcd. for C.sub.26H.sub.39NO.sub.6S, 493.2498).
[0025] Epothilone C.sub.9 (26): colorless amorphous solid; [.alpha.].sup.22D -75.2 (c 2.5, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 210 (16803), 248 (17800); IR (KBr) .nu..sub.max 3443, 2932, 2881, 1734, 1689, 1465, 1255,1183, 976 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.93 (1H, s, H-19), 6.62 (1H, dd, J=15.6, 0.6 Hz, H-17), 6.49 (1H, dd, J=15.6, 6.6 Hz, H-16), 5.52 (1H, dddd, J=9.5, 6.6, 2.8, 0.6 Hz, H-15), 5.42 (1H, m, H-12), 5.41 (1H, m, H-13), 4.13 (1H, ddd, J=11.0, 5.3, 2.8 Hz, H-3), 3.69 (1H, ddd, J=3.7, 2.8, 2.5 Hz, H-7), 3.11 (1H, dq, J=2.5, 6.8 Hz, H-6), 2.95 (1H, d, J=5.3 Hz, 3-OH), 2.90 (1H, d, J=2.8 Hz, 7-OH), 2.69 (3H, s, H-21), 2.67 (1H, ddd, 0.1=14.9, 9.5, 8.4 Hz, H-14a), 2.48 (1H, dd, J=15.6, 11.0 Hz, H-2a), 2.33 (1H, dd, J=15.6, 2.8 Hz, H-2b), 2.30 (1H, bd, J=14.9 Hz, H-14b), 2.14 (1H, m, H-11a), 2.03 (1H, m, H-11b), 1.71 (1H, m, H-8), 1.63 (1H, m, H-10a ), 1.31 (1H, m, H-9a), 1.29 (3H, s, H-23), 1.17 (3H, d, J=6.8 Hz, H-24), 1.16 (1H, m, H-10b), 1.14 (1H, m, H-9b), 1.05 (3H, s, H-22), 0.97 (3H, d, J=7.1 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (21), 310 (10), 276 (21), 171 (83), 154 (100), 150 (27), 111 (18); HREIMS m/z 463.2382 (calcd. for C.sub.25H.sub.37NO.sub.5S, 463.2392).
[0026] Epothilone C.sub.9, (27): colorless amorphous solid; [.alpha.].sup.22D-93.4 (c 1.0, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 209 (15200), 254 (15700); IR (KBr)) .nu..sub.max 3416, 2966, 2932, 1736, 1689, 1463, 1249, 1011 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 7.06 (1H, s, H-19), 6.65 (1H, bs, H-1 7), 6.56 (1H, dd, J=10.6, 4.4 Hz, 27-OH), 5.55 (1H, d, J=6.2 Hz, 3-OH), 5.52 (1H, dd, J=11.6, 2.0 Hz, H-15), 5.44 (1H, dddd, J=11.2, 10.7, 3.1, 1.7 Hz, H-12), 5.35 (1H, dddd, J=11.0, 10.7, 3.9, 1.7 Hz, H-13), 4.47 (11-1, ddd, J=12.5, 4.4, 1.3 Hz, H-27a), 4.35 (1H, ddd, J=11.7, 6.2, 2.6 Hz, H-3), 4.20 (1H, ddd, J=12.5, 10.6, 0.9 Hz, H-27b), 3.63 (1H, ddd, J=4.6, 1.8, 0.9 Hz, H-7), 3.24 (1H, d, J=1.8 Hz, 7-OH), 3.13 (1H, dq, J=0.9, 6.8 Hz, H-6), 2.80 (1H, ddd, J=14.8, 11.6, 11.0 Hz, H-14a), 2.71 (3H, s, H-21), 2.40 (1H, dd, J=14.4, 11.7 Hz, H-2a), 2.24 (1H, m, H I 1a), 2.06 (1H, dd, J=14.4, 2.6 Hz, H-2b), 2.01 (1H, ddd; J=14.8, 3.9, 2.0 Hz, H-14b), 2.00 (1H, m, H-1 lb), 1.77 (1H, m, H-8), 1.69 (1H, m, H-10a ), 1.35 (1H, m, H-9a), 1.35 (3H, s, H 23), 1.19 (1H, m, H-10b), 1.19 (3H, d, J=6.8 Hz, H-24), 1.18 (1H, m, H-9b), 1.01 (3H, d, J=7.1 Hz, H-25), 0.98 (3H, H-22); .sup.13C NMR, see Table 1; EIMS m/z 493 [M].sup.+ (17), 306'(64), 184 (50), 171 (30), 167 (38), 166 (100), 138 (12); HREIMS m/z 493.2502 (calcd. for C.sub.26H.sub.39NO.sub.6S, 493.2498).
[0027] trans-Epothilone C.sub.1, (28): colorless amorphous solid; [.alpha.].sup.22D -84 (c 0.2, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 211 (17400), 248 (12900);1R (KBr) .nu..sub.max 3433, 2961, 2933, 2879, 1730, 1708, 1457, 1251, 975 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 600 MHz) .delta. 7.00 (1H, s, H-19), 6.64 (1H, bs, H17), 5.45 (1H, ddd, J=15.2, 6.5, 6.5 Hz, H-12), 5.42 (1H, dd, J=6.4, 3.7 Hz, H-15), 5.35 (1H, dt, J=15.2, 7.1 Hz, H-13), 4.42 (1H, m, H-3), 3.58 (1H, ddd, J=8.1, 7.9, 2.8 Hz, H-7), 3.24 (1H, m, H-6), 3.14 (1H, dq, J=4.0, 6.9 Hz, H-6), 2.92 (1H, d, J=7.9 Hz, 7-OH), 2.71 (3H, s, H-21), 2.71 (2H, m, H-2), 2.53 (2H, m, H-14), 2.17 (1H, d, J=2.17 Hz, 3-OH), 2.11 (1H, m, H-11a), 2.06 (3H, bs, H-27), 1.93 (1H, m, H-1 lb), 1.68 (1H, m, H-9a), 1.65 (1H, m, H-10a ), 1.33 (1H, m, H-8), 1.26 (3H, d, J=6.8 Hz, H-24), 1.16 (1H, m, H-10b), 1.12(3K d, J=6.9 Hz, H-22), 1.07 (1H, m, H-9b), 1.00 (3H, d, J=6.8 Hz, H-25); .sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (6), 290 (21), 289 (20), 204 (23), 194 (19), 190 (22), 168 (100), 164 (48), 157 (14), 152 (19), 151 (17), 139 (15), 111 (18); HREIMS m/z 463.2371 (calcd. for C.sub.25H.sub.37NO.sub.5S, 463.2392).
[0028] trans-Epothilone C.sub.2 (29): colorless amorphous solid; [.alpha.].sup.22D -3 (c 1.5, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 211 (15800), 248 (11900); IR (KBr) .nu..sub.max3435, 2963, 2931, 2878, 1731, 1706, 1457,1273, 979 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 600 MHz) .delta. 6.99 (1H, s, H-19), 6.57 (1H, bs, H 17), 5.56*(] H, ddd, J=15.1, 7.4, 7.0 Hz, H-12), 5.41 (1H, ddd, J=15.1, 7.0, 6.9 Hz, H-13), 5.41 (1H, dd, J=7.7, 2.8 Hz, H-15), 4.13 (1H, dddd, J=6.7, 6.2, 5.6, 5.1 Hz, H-3), 3.78 (1H, ddd, J=8.2, 6.5, 1.9 Hz, H-7), 3.18 (1H, d, J=5.6 Hz, 3-OH), 3.06 (1H, dq, J=8.2, 7.1 Hz, H-6), 2.98 (1H, dq, J=6.2, 7.0 Hz, H-4), 2.71 (3H, s, H-21), 2.64 (1H, dd, J=15.1, 6.7 Hz, H2a), 2.54 (1H, dd, J=15.1, 5.1 Hz, H-2b), 2.44 (2H, m, H-14), 2.22 (1H, dddd, J=13.8, 7.0, 6.2, 2.9 Hz, H-11a), 2.10 (3H, d, J=1.1 Hz, H-27), 2.09 (H, d, J=6.5 Hz, 7-OH), 1.88 (1H, dddd, J=13.8, 10.9, 7.4, 2.9 Hz, H-1 lb), 1.65 (1H, m, H-8), 1.63 (1H, m, H-10a ), 1.56 (1H, dddd, J=12.7, 12.7, 3.9, 3.9 Hz, H-9a), 1.20 (3H, d, J=7.1 Hz, H-24), 1.15 (3H, d, J=7.0 Hz, H-23), 1.13 (1H, m, H-10b), 1.04 (1H, m, H-9.b), 1.01 (3H, d, J=7.0.Hz, H-25);.sup.13C NMR, see Table 1; EIMS m/z 463 [M].sup.+ (13), 290 (11), 190 (10), 168 (100), 164 (20), 157 (26), 139 (17); HREIMS m/z 463.2383 (calcd. for C.sub.25H.sub.37NO.sub.5S, 463.2392).
[0029] Epothilone I.sub.1, (30): colorless amorphous solid; [.alpha.].sup.22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max f nm (.epsilon.) XXX; IR(KBr) .nu..sub.maxXXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) 6.96 (1H, s, H-1 9), 6.54 (1H, bs, H-1 7), 5.49 (Iii, ddd, J=10.3, 7.3, 7.3 Hz, H-12), 5.33 (1H, dd, J=8.3, 4.4 Hz, H-15), 5.31 (1H, m, H-133), 4.15 (1H, ddd, J=8.0, 5.0, 4.6 Hz, H-3), 3.80 (1H, m, H-7), 3.21 (1H, dq, J=6.0, 6.9 Hz, H-6), 2.89 (1H, d, J=5.0 Hz, 3-OH); 2.70 (3H, s, H-21), 2.65 (1H, ddd, J=15.8, 8.5, 8.3 Hz, H-14a), 2.42 (2H, m, H-2), 2.35 (1H, m, H-14b), 2.27 (1H, bd, 0.1=3.3 Hz, 7-OH), 2.13 (1H, m, H-11a), 2.09 (3H, d, J=1.2 Hz, H-27), 2.00 (1H, m, H-1 lb), 1.72 (1H, m, H-8), 1.40 (2H, m, H-10p), 1.37 (1H, m, H-9pa), 1.36 (2H, m, H-9a), 1.32 (3H, s, .H-23), 1.27 (3H, m, H-9b and H-10a), 1.13 (3H, d, J=6.9 Hz, H-24), 1.09 (3H, s, H-22), 0.94 (3H, d, J=6.9 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 75 MHz) .delta. 221.3 (s, C-5), 171.1 (s, C-1), 164.8 (s, C-20), 152.4 (s, C-18), 137.4 (s, C-16), 133.8 (d, C-12), 124.6 (d; C-13), 120.0 (d, C-17), 116.2 (d, C-19), 78.8 (d, C-15), 74.9 (d, C-7), 74.7 (d, C-3), 51.6 (s, C-4), 43.7 (d, C-6), 38.9 (t, C-2), 34.3 (d, C-8), 31.6 (t, C-14), 29.3 (t, C-9a, 28.6 (t, C-10.sub..beta.), 28.2 (t, C-10.sub.a), 26.6 (t, C-11), 24.8 (t, C-9.sub..beta.), 23.6 (q, C-22), 19.3 (q, C23), 19.3 (q, C-21), 16.5 (q, C-25), 15.5 (q, C:27), 13.7 (q, C-24); EIMS m/z 505 [M].sup.+ XXX; HREIMS m/z 505.XXX (calcd. for C.sub.28H.sub.43NO.sub.5S, 505.XXX).
[0030] Epothilone I.sub.2, (31): colorless amorphous solid; [.alpha.].sup.22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX; IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .alpha. 6.95 (1H, s, H-19), 6.53 (1H, bs, H-17), 5.40 (1H, m, H-12), 5.38 (1H, dd, J=9.8, 3.3 Hz, H-15), 5.37 (1H, m, H-133).4.21 (1H, ddd, 0.1=8.6, 3.8, 3.6 Hz, H-3), 3.85 (1H, ddd, J=8.5, 5.8, 2.2 Hz, H7), 3.18 (1H, dq, J=8.5, 7.0 Hz, H-6), 2.70 (3H, s, H-21), 2.65 (1H, ddd, J=15.2, 9.8, 9.0 Hz, H-14a), 2.51 (1H, d, J=3.6 Hz, 3-OH), 2.37 (2H, m, H-2), 2.32 (1H, bd, J=15.2 Hz, H-14b), 2.09 (3H, d, J=1.3 Hz, H-27), 2.07 (2H, m, H-11), 1.78 (1H, m, H-8), 1.65 (1H, d, J=5.8 Hz, 7-OH), 1.57 (1H, m, H-10.sub..beta.a), 1.44 (1H, m, H-10.sub..alpha.a), 1.42 (1H, m, H-9.sub..beta., 1.32 (3H, s, H-23), 1.21 (1H, m, H-10.sub..beta.b), 1.17 (3H, d, J=7.0 Hz, H-24), 1.13 (2H, m, H-9.sub..alpha.),1.06 (3H, s, H-22), 0.95 (3H, d, J=7.0 Hz, H-25.sub..alpha.), 0.91 (3H, d, J=6.5 Hz, H-25.sub..beta.), 0.68 (1H, m, H-10.b); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 220.4 (s, C-5), 171.3) (s, C-1), XXX (s, C-20), 152.4 (s, C-18), 137.6 (s, C-16), 13)4.5 (d, C-12), 125.3) (d, C-13), 119.6 (d, C-17), 116.2 (d, C-19), 78.6 (d, C-15), 77.2 (d, C-7), 75.0 (d, C-3), 51.0 (s, C-4), 44.6 (d, C-6), 38.2 (t, C-2), 36.9 (t, C-9.sub..alpha.), 34.5 (t, C-10.sub..alpha.), 32.6 (d, C-8), 32.0 (t, C-14), 30.0 (d, C-9.sub..beta.), 27.4 (t, C-11), 26.6 (t, C-10p), 25.0 (q, C-22), 21.5 (q, C-25.sub..beta.), 19.3 (q, C-21), 17.9 (q, C-25.sub..alpha.), 17.7 (q, C-23), 15.8 (q, C-24), 15.6 (q, C-27); EIMS m/z 519 MV XXX; HREIMS ,n/. 519.XXX (calcd. for C.sub.29H.sub.45NO.sub.5S, 519.XXX).
[0031] Epothilone I.sub.3 (32): colorless amorphous solid; [.alpha.].sup.22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX; IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.95 (1H, s, H-19), 6.52 (1H, bs, H-17), 5.32 (1H, dd, J=9.1, 3.0 Hz, H-15), 5.08 (1H, dd, J=8..about., 3.9 Hz, H-13), 4.13 (1H, ddd, J=9.4,4.3, 3.2 Hz, H-3), 3.81 (1H, m, H-7), 3.18 (1H, dq, J=6.8, 7.0 Hz, H-6), 2.83 (1H, d, J=4.3 Hz, 3-OH), 2.70 (3H, s, H-21), 2.61 (1H, ddd, J=15.8,9.1, 8.5 Hz, H-14 a), 2.43 (1H, dd, J=14.0, 3.2 Hz, H-2a), 2.3 8 (2H, dd, J=14.0, 9.4 Hz, H-2b), 2.30 (1H, bd, J=15.8 Hz, H-14b), 2.16 (1H, ddd, J=14.1, 8.3, 7.4 Hz, H-11a), 2.08 (3H, d, J=1.0 Hz, H-27), 1.99 (1H, d, J=4.7 Hz, 7-OH), 1.92 (1H, ddd, J=14.1, 6.3, 6.3 Hz, H-1 Ib), 1.82 (1H, m, H-8), 1.67 (3H, s, H-26), 1.51 (1H, m, H-10pa), 1.40 (1H, m, H-9.sub..beta.), 1.33 (1H, m, H-10.sub..beta.b), 1.31 (3H, s, H-23), 1.27 (1H, M H-10.sub..alpha.a), 1.23 (1H, m, H-9.sub..alpha.a), 1.16 (3H, d, J=7.0 Hz, H-24), 1.10 (1H, m, H-9.sub..alpha.b), 1.07 (3H, s, H-22), 0.95 (3H, d, J=7.0 Hz, H-25.sub..alpha.), 0.92 (3H, d, J=6.5 Hz, H-25.sub..beta.), 0.75 (1H, m, H-10.sub..alpha.b); EIMS m/z 533 M+XXX; HREIMS m/z 533.XXX (calcd. for C.sub.30H.sub.47NO.sub.5S, 533.XXX).
[0032] Epothilone 14 (33): colorless amorphous solid; [.alpha.].sup.22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX; IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.95 (1H, s, H-19), 6.53 (1H, bs, H-17), 5.47 (1H, dt, J=11.1, 5.8 Hz, H-12), 5.33 (1H, ddd, J=9.2, 3.9, 0.5 Hz, H-15), 5.33 (1H, m, H-13), 4.09 (1H, dddd, J=9.6, 8.1, 4.5, 3.3 Hz, H-3), 3.83 (1H, m, H-7), 3.57 (1H, bs, 3-OH), 2.89 (1H, dq, J=7.4, 7.1 Hz, H-6), 2.83 (1H, dq, J=8.1, 7.1 Hz, H-4), 2.70 (3H, s, H-21), 2.64 (1H, m, H-14a), 2.42 (1H, dd, J=14.2, 3.3 Hz, H-2a), 2.43 (1H, dd, J=14.2, 9.6 Hz, H-2b), 2.30 (1H, m, H-14b), 2.10 (3H, d, J=1.3 Hz, H-27), 2.09 (2H, m, H-11), 1.81 (1H, m, H-8), 1.74 (1H, bd, J=5.6 Hz, 7-OH), 1.53 (1H, m, H-10.sub..beta.a), 1.49 (1H, m, H-9.sub..beta.), 1.47 (1H, m, H-10.sub..alpha.a), 1.27 (1H, m, H-10.sub..beta.b), 1.24 (1H, m, H-9.sub..alpha.a) 1.17 (3H, d, J=7.1 Hz, H-23), 1.14 (1H, m, H-9.sub..alpha.b) 1.08 (3H, d, J=7.1 Hz, H-24), 0.97 (3H, d, J=6.9 Hz,H-25.sub..alpha.), 0.91 (3H, d, J=6.5 Hz, H-25.sub..beta.), 0.79 (1H, m, H-10.sub..alpha.b); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 217.0 (s, C-5), 170.8 (s, C-1), 164.8 (s, C-20), 152.4 (s, C-18), 137.1 (s, C-16), 134.6 (d, C-12), 124.7 (d, C-13), 120.2 (d, C-17), 116.4 (d, C-19), 78.7 (d, C-15), 76.4 (d, C-7), 71.3 (d, C-3), 50.7 (d, C4), 50.1 (d, C-6), 40.7 (t, C-2), 38.5 (t, C-9.sub..alpha.), 35.5 (t, C-10.sub..alpha.), 33.4 (d, C-8), 31.8 (t, C-14), 30.0 (d, C-9.sub..beta.), 27.2 (t, C-11), 26.7 (t, C-10.sub..beta.), 21.4 (q, C-25.sub..beta.p), 19.3 (q, C-21), 18.2 (q, C-25.sub..alpha.), 15.4 (q, C-27), 14.4 (q, C-24), 13.1 (q, C-23); EIMS m/z 505 [M].sup.+ XXX; HREIMS m/z 505.XXX (calcd. for C.sub.28H.sub.43NO.sub.5S, 505.XX.X).
[0033] Epothilone 15, (34): colorless amorphous solid; [.alpha.]22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX; IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.97 (1H, s, H-19), 6.52 (1H, bs, H-17), 5.32 (1H, dd, J=7.1, 6.2 Hz, H-15), 5.03 (1H, dd, J=8.4, 5.0 Hz, H-13), 4.05 (1H, dddd, J=7.5, 7.2, 5.9, 4.6 Hz, H-3), 3.91 (1H, m, H-7), 3.17 (1H, d, J=5.9 Hz, 3-OH), 2.94 (1H, d q, J=7.2, 7.1 Hz, H-4), 2.87 (1H, dq, J=6.5, 6.9 Hz, H-6), 2.70 (3H, s, H-21), 2.62 (1H, dd, J=14.6, 4.6 Hz, H-2a), 2.60 (1H, M, H-14a), 2.53 (1H, dd, J=14.6, 7.5 Hz, H-2b), 2.31 (1H, m, H-14b), 2.10 (3H, d, J=1.1 Hz, H-27), 2.10 (1H, M, H-11a), 2.02 (1H, m, H-I I b), 1.97 (1H, bd, 1=5.6 Hz, 7-OH), 1.84 (1H, m, H-8), 1.66 (3H, s, H-26), 1.55 (1H, m, H-9p) 1.49 (1H, m, H-100a), 1.39 (1H, m, H-100b), 1.33. (1H, m, H-10.sub..alpha.a), 1.31 (1H, m, H-9.sub..alpha.), 1.15 (3H, d, J=7.1 Hz, H-23), 1.12 (1H, m, H-9.sub..alpha.b), 1.11 (3H d, J=6.9 Hz, H-24), 0.97 (3H, d, J=6.9 Hz,H-25.sub..alpha.), 0.94 (1H, m, H-10.sub..alpha.b), 0.93 (3H, d, J=6.6 Hz, H-25p); EIMS m/z 519 .mu.l+XXX; HREIMS ink 519.XXX (calcd. for C.sub.29H.sub.45NO.sub.5S,519.XXX).
[0034] Epothilone I.sub.6 (35): colorless amorphous solid; [C].sup.22D -XXX (c XXX, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) XXX; IR (KBr) .nu..sub.max XXX cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.97 (1H, s, H-19), 6.52 (1H, bs, H-1 7), 5.24 (1H, dd, J=6.9, 6.9 Hz, H-15), 5.02 (1H, dd, J=8.8, 5.2 Hz, H-13), 4.22 (1H, tdd, J=6.1, 5.6, 4.8 Hz, H-3), 3.76 (1H, ddd, J=6.1, 5.7, 5.6 Hz, H-7), 3.13 (1H, d, J=5.6 Hz, 3-OH), 3.05 (1H, dq, J=4.8, 7.0 Hz, H-4), 2.79 (1H, dq, J=5.6, 6.9 Hz, H-6), 2.70 (3H, s, H-21), 2.62 (1H, m, H-14a), 2.57 (2H, d, J=6.1 Hz, H-2a), 2.30 (1H, m, H-14b), 2.08 (3H, d, J=1.0 Hz, H-27), 2.02 (2H, m, H-11), 1.73 (1H, d, J=6.1 Hz, 7-OH), 1.69 (1H, m, H-8), 1.66 (3H, s, H-26), XXX (H-9.sub..alpha., H-9.sub..beta., H-10.sub..alpha., H-10.sub..beta.), 1.21 (3H, d, J=7.0. Hz, H-22), 1.16 (3H, d, J=6.9 Hz, H-24), 0.94 (3H, d, J=6.9 Hz,H-25.sub..alpha.), 0.91 (3H, d, J=6.4 Hz, H-25.sub..beta.); EIMS m/z 519 [M].sup.+ XXX; HREIMS m/z 519.XXX (calcd. for C.sub.29H.sub.45NO.sub.5S, 519.XXX).
[0035] Epothilone K (36): colorless amorphous solid; [.alpha.].sup.22D -7 (c 0.08, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 212 (16700), 248 (12500); IR (KBr) .nu..sub.max3431, 2963, 2927, 2856, 1731, 1712, 1262, 1093, 1021, 802 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.95 (1H, s, H-19), 6.51 (1H, bs, H-17), 5.49 (3H, m, H-15, H-13, and H-12), 4.04 (1H, dddd, J=7.9, 7.6, 6.9, 3.3 Hz, H-3), 3.36 (1H, dq, J=6.9, 6.8 Hz, H-6), 2.83 (1H, d, J=7.6 Hz, 3-OH), 2.75 (1H, ddd, J=16.1, 6.6, 3.4 Hz, H-14a), 2.74 (1H, dd, J=15.3, 3.3 Hz, H-2a), 2.71 (3H, s, H-21), 2.58 (2H, m, H-14b and H-8), 2.50 (1H, dd, J=15.3, 7.9 Hz, H-2b), 2.29 (1H, m, H-11a), 2.10 (1H, m, H-1 lb), 2.09 (311, d, J=0.7 Hz, H-27), 1.78 (1H,-m, H-9a), 1.65 (1H, m, H-10a ), 1.48 (1H, m, H-10b), 1.18 (1H, m, H-9b), 1.15 (3H, d, J=6.8 Hz, H-22), 1.03 (3H, d, J=6.5 Hz, H-25); EIMS m/z 405 [M].sup.+ (38), 317 (12), 260 (9), 232 (10), 204 (14), 190 (16), 168 (100), 164 (30), 151 (28); HREIMS m/z 405."X (calcd. for C.sub.26H.sub.39NO.sub.5S, 405.XXX).
[0036] (37): colorless amorphous solid; [.alpha.]22D -27.5 (c 0.4, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 211 (16100), 247 (12100); IR (KBr) .nu..sub.max 3431, 2967, 2929, 2875, 1704, 1462, 1381, 1010 cm.sub.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.94 (1H, s, H-19), 6.55 (1H, bs, H-17), 5.56 (1H, dtt, J=10.8, 7.3, 1.4 Hz, H-12), 5.39 (1H, dtt, J=10.8, 7.3, 1.4 Hz, H-13), 4.17 (1H, t, J=6.6 Hz, H-15), 3.50 (1H, ddd, J=8.7,2.6,2.6 Hz, H-7), 3.10 (1H, d, J=2,6,7-OH), 2.90 (1H, dq, J=2.6, 7.2 Hz, H-6), 2.77 (1H, sep, J=6.9 Hz, H-4), 2.70 (3H, s, H-21), 2.40 (2H, m, H-14), 2.07 (2H, m, H-11), 2.04 (3H, d, J=1.1 Hz, H-27), 1.78 (1H, bs, 15-OH), 1.74 (1H, m, H-9a),.1.50 (1H, m, H-8), 1.46 (1H, m, H-10a ), 1.27 (1H, m, H-10b), 1.11 (1H, m, H-9b), 1.094 (3H, d, J=6.9 Hz, H-23), 1.089 (3H, d, J=6.9 Hz, H-22), 1.08 (3H, d, J=7.2 Hz, H-24), 0.82 (3H, d, J=6.7 Hz, H-25); .sup.13C NMR (CDCl.sub.3, 100 MHz) .delta. 220.5 (s, C-5), 164.6 (s, C-20), 152.9 (s, C-18), 141.5 (s, C-16), 133.4 (d, C-12), 125.0 (d, C-13), 119.2 (d, C-17), 115.6 (d, C-19), 77.2 (d, C-15), 74.9 (d, C-7), 44.9 (d, C-6), 40.0 (d, C-4), 35.5 (d, C-8), 33.5 (t, C-14), 32.3 (t, C-9), 27.9 (t, C-11), 26.9 (t, C-10), 19.2 (q, C-21), 18.6 (q, C-23), 18.1 (q, C-22), 15.6 (q, C-25), 14.4 (q, C-27), 9-3 (q, C-24); EIMS m/z 407 [M].sup.+ (0.1), 204 (0.8), 168 (100), 140 (3.4); HREIMS m/z 407.XXX (calcd. for C.sub.23H.sub.37NO.sub.3S, 407.XXX).
[0037] (38): colorless amorphous solid; [.alpha.].sup.22D+25.0 (c 0.5, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 212 (17700), 247 (13400); IR (KBr) .nu..sub.max 3427, 2971, 2933, 2878, 2858, 1709, 1457, 1377, 1186,1023 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 6.95 (1H, s, H-19), 6.55 (1H, bs, H-17), 5.52 (1H, dtt, J=10.9, 7.2, 1.4 Hz, H-12), 5.39 (1H, dtt, J=10.9, 7.1, 1.2 Hz, H-13), 4.18 (1H, ddt, J=3.4, 0.4, 6.7 Hz, H-1 5), 2.71 (3H, s, H-21), 2.51 (1H, bq, J=6.8 Hz, H-8), 2.48 (1H, dq, J=17.7, 7.4 Hz, H-6a), 2.41 (1H, dq, 1=17.7, 7.2 Hz, H-6b), 2.39 (2H, ddd, J=7.1, 6.7, 1.4 Hz, H-14), 2.06 (2H, ddt, 7.2, 1.2, 7.0 Hz, H-11), 2.05 (3H, d, J=1.4 Hz, H-27), 1.81 (1H, d, J=3.4 Hz, 15-01-1), 1.66 (1H, m, H-9a), 1.32 (1H, m, H-9b), 1.31 (2H, m, H-10), 1.06 (3K d, J=6JHz, H-25), 1.04 (3H, dd, J=7.4, 7.2 Hz, H-24); .sup.13C NMR (CDCl.sub.3, 75 MHz) .delta. 215.3 (s, C-7), 164.6 (s, C-20), 152.9 (s, C-18), 141.5 (s, C-16), 132.7 (d, C-12), 125.3 (d, C-13), 119.2 (d, C-17), 115.6 (d, C-19), 77.2 (d, C-15), 46.0 (d, C-8), 34.3 (t, C-14), 33.5 (t, C-6), 32.7 (t, C-9), 27.5 (t, C-11), 27.3 (t, C-10), 19.2 (q, C-21), 16.5 (q, C-25), 14.4 (q, C-27), 7.8 (q, C-24); EIMS m/z 335 [M].sup.+ (2), 317 (4), 170 (27), 169 (67), 168 (100), 140 (20); HREIMS m/z 335.1912 (calcd. for C.sub.19H.sub.29NO.sub.2S, 335.1919).
[0038] (39): colorless amorphous solid; [.alpha.].sup.22D+26.4 (c 0.27, MeOH); UV (MeOH) .lambda..sub.max nm (.epsilon.) 203 (19100), 244 (12500); IR (KBr) .nu..sub.max3430, 2970, 2934, 2877, 1710, 1458, 1377, 1184 cm.sup.-1; .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 6.94 (1H, s, H-19), 6.55 (1H, bs, H-17), 5.17 (1H, t, J=7.3 Hz, H-13), 4.13 (1H, m, H-15), 2.70 (3H, s, H-21), 2.51 (1H, bq, J=6.8 Hz, H-8), 2.47 (1H, dq, J=17.7, 7.2 Hz, H-6a), 2.41 (1H, dq, J=17.7, 7.2 Hz, H-6b), 2.33 (2H, bdd, J=7.3, 6.8 Hz, H-14), 2.05 (3H, d, J=1.2 Hz, H-27), 2.03 (2H, m, H-11), 1.71 (1H, d, J=3.2 Hz, 15-OH), 1.69 (3H, d, J=1.3 Hz, H-26), 1.62 (1H, m, H-9a), 1.32 (3H, m, H-10 and H-9b), 1.06 (3H, d, J=6.9 Hz, H-25), 1.03 (3H, t, J=7.2 Hz, H-24); EIMS m/z 349 [M].sup.+ (0.7), 331 (1.7), 168 (100), 140 (5.1); HREIMS m/., 349.XXX (calcd. for C.sub.20H.sub.31NO.sub.2S, 349.XXX).
1TABLE 1 Activity of epothilones and compounds (1) to (39) against mouse fibroblasts (L929, IC 50/ng/ml) Epothilone Structural type A.gamma. B.gamma. C.gamma. D.gamma. trans C.gamma. Starting (1) 4 (2) 1-2 (14) 50-100 (15) 20 -- epothilone 21-Hydroxy (3) 10 (4) 1.5 -- -- -- (E&F) Oxozoles (10) 6 (11) 1 (12) 120 (13) 11 -- (G&H) (R)-4- (5) 20 -- (16) 200 (17) 20 (28) 400 Desmethyl (X.sub.1) (S)-4- (6) 7 -- (10) 25-30 (19) 12 (29) 80 Desmethyl (X.sub.2) 6-Desmethyl -- -- (20 1500 -- -- (X.sub.3) 8-Desmethyl -- -- (21) 800 -- -- (X.sub.4) 8,9-Dehydro -- -- (22) 1500 (23) 200 (X.sub.5) 10,11-Dehydro -- -- (24) 120 -- -- (X.sub.6) 14-Hydroxy -- -- (25) -- -- (X.sub.7) 16-Desmethyl (7) 20 -- (26) 250 (X.sub.8) 27-Hydroxy (8) 100 -- (27) 200 -- -- (X.sub.9) 21-Methyl -- (9) 1.5 -- -- -- (X.sub.10) Compound -- -- (36) 180 Compound -- -- (37) 50 -- -- Compound -- -- (38) 2000 (39) 500 --
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