U.S. patent application number 10/103840 was filed with the patent office on 2003-09-25 for process for biological deinking of office waste paper.
Invention is credited to Bharathi, P.A. Loka, Chandramohan, D., Mohandass, C., Nair, Shanta, Oliviera, Telma, Raghukumar, Chandralata, Raghukumar, Seshagiri.
Application Number | 20030178162 10/103840 |
Document ID | / |
Family ID | 28040472 |
Filed Date | 2003-09-25 |
United States Patent
Application |
20030178162 |
Kind Code |
A1 |
Raghukumar, Chandralata ; et
al. |
September 25, 2003 |
Process for biological deinking of office waste paper
Abstract
This invention relates to a biological process for deinking of
office waste paper and more particularly, the present invention
relates to a biological process for deinking of xerographic and
inkjet printed paper using a gram-negative coccoid bacterium
deposited at National Institute of Oceanography, Dona Paula, Goa
403 043, India having accession No. NIO/DI/32 and being deposited
at an international depository ______ having accession number
______ and also deposited at Microbial Technology, Chandigarh,
India having accession No. ______.
Inventors: |
Raghukumar, Chandralata;
(Goa, IN) ; Mohandass, C.; (Goa, IN) ;
Oliviera, Telma; (Goa, IN) ; Raghukumar,
Seshagiri; (Goa, IN) ; Chandramohan, D.; (Goa,
IN) ; Bharathi, P.A. Loka; (Goa, IN) ; Nair,
Shanta; (Goa, IN) |
Correspondence
Address: |
Thomas T. Moga
DICKINSON WRIGHT PLLC
1901 L STREET N.W.
SUITE 800
WASHINGTON
DC
20036
US
|
Family ID: |
28040472 |
Appl. No.: |
10/103840 |
Filed: |
March 25, 2002 |
Current U.S.
Class: |
162/5 ; 162/72;
510/174 |
Current CPC
Class: |
D21C 5/027 20130101;
D21C 5/005 20130101; C12P 1/04 20130101; Y02W 30/648 20150501; Y02W
30/64 20150501 |
Class at
Publication: |
162/5 ; 162/72;
510/174 |
International
Class: |
D21C 005/02; D21C
003/20 |
Claims
1. A process for biological deinking of office waste paper
inclusive of xerographic and inkjet-printed paper printed with
non-impact and non-dispersible ink, said process comprises: (a)
growing coccoid bacterial isolate deposited at National Institute
of Oceanography, Dona Paula, Goa 403 043, India having accession
No. NIO/DI/32 and being deposited at an international depository
______ having accession number ______, in a nutrient broth
containing beef extract, peptic digest of animal tissue and
salinity ranging between 30 to 35 parts per thousand for at least 4
days; (b) adding the resulting biomass of step (a) at 20%
concentration to inoculate paper pulp diluted to at least 6%
consistency suspended in sea water with salinity diluted to 50%;
(c) incubating the inoculated pulp for a minimum period of 96 hours
followed by diluting the pulp tenfold; (d) adding 1% by wt. of a
surfactant and aerating for at least 10 minutes with compressed air
to let the stray residual ink particles and contaminants flow out
of the container, and (e) filtering the pulp of step (d) over a
funnel under suction, to get a sheet of pulp with uniform thickness
and reading the brightness by radiance measurement.
2. A process as claimed in claim 1, wherein in step (a), the
bacterium is grown for a minimum of 4 days in nutrient broth
containing beef extract and peptic digest of animal tissue in sea
water with salinity diluted to 50%.
3. A process as claimed in claim 1, wherein in step (b), the
resulting bacterial biomass is concentrated by centrifugation and
diluted to get final optical density of 1.0 measured at 660 nm in a
spectrophotometer.
4. A process as claimed in claim 1, wherein the diluted bacterial
culture is inoculated to at least 6% pulp suspended in seawater
with salinity diluted to 50% and incubated for a minimum period of
4 days within to completely beach the pulp.
5. A process as claimed in claim 1, wherein the resulting bleached
pulp is diluted tenfold, to which 1% surfactant is added, aerated
using compressed air, and any free residual ink particles and
contaminants are allowed to flow out of the solution.
6. A process as claimed in claim 1, wherein after step (d) the
xerographic or inkjet-printed paper is soaked in hot water for 1-2
hours and macerated after adding 1% surfactant to pulp.
7. A process as claimed in claim 6, wherein the paper is macerated
using conventional mixers.
8. A process as claimed in claim 1, wherein in step (e), the washed
pulp is filtered over a Buchner funnel under suction to get a pulp
sheet of uniform thickness.
9. A process as claimed in claim 1, wherein the pulp sheet is
pressed at 60.degree. C. for a minimum of 4-5 hours to make it
uniform and dried.
10. A process as claimed in claim 1, wherein the radiance of the
resultant sheet is read from 412 to 684 nm and the radiance
expressed as Lux units (LU) {wherein one LU is equivalent to
.mu.W/cm.sup.2/nm/Sr}.
11. A process as claimed in claim 1, wherein the pulp sheet made
out of recycled paper is used as a reference.
12. A process as claimed in claim 1, wherein in steps (a) and (b),
pure water containing 1.5% by wt of sodium chloride can be used in
the place of sea water.
Description
FIELD OF THE INVENTION
[0001] The present invention relates to decolorization of office
waste paper using microorganisms from marine habitats. More
particularly, the present invention relates to a process for
biological deinking of photocopied and inkjet-printed paper or
toner photocopied paper pulp using a gram-negative coccoid
bacterium deposited at National Institute of Oceanography, Dona
Paula, Goa 403 043, India having accession No. NIO/DI/32 and being
deposited at an international depository ______ having accession
number ______ and also deposited at Microbial Technology,
Chandigarh, India having accession No. ______.
BACKGROUND OF THE INVENTION
[0002] Paper manufacturing is a major industry and a continuously
growing one. Increased production of paper imposes a severe demand
on plant raw material and thus harms the environment. Recycling of
used paper is an alternative that can alleviate the stress that is
exercised on the environment. The three major sources of raw
material for such recycling are newsprint, photocopied paper and
inkjet--printed papers. Recycling of paper requires the removal of
the printing ink from the used paper, called deinking, so that the
processed material is brighter. Printing on paper is accomplished
by using two types of inks, the impact and the non-impact inks. In
impact inks, used for newsprints, the ink does not fuse with the
paper and is, therefore, easy to remove or disperse during the
deinking, or recycling process. Such recycling is now well known
and has been carried out for years. On the contrary, non-impact
inks used in photocopying, ink-jet printing and laser printing
results in the ink fusing with the paper and makes it
non-dispersible, thus rendering the deinking process much more
difficult (Jeffries, 2002). The toners used for photocopying
generally contain iron oxide (about 55%), olefins (about 5%) and
plastic or polymer (Website:http://www.eng-tips.com).
[0003] Various hydrolytic enzymes such as cellulase, lipase,
amylase of bacterial or fungal origin, individually or in
combination have been used for deinking of office waste paper.
[0004] (i) A reference may be made to a publication wherein, a
method for deinking of xerographic and printed paper, collectively
called office waste, includes applying deinking solution to the
paper to be deinked, the deinking solution being composed of a
cleaning solution and a surfactant, abrading the paper to remove
the ink from paper, and washing the paper to remove the deinking
solution from the paper (U.S. Pat. No. 6,022,423 Bhatia, S. 1996
Method for deinking paper dtd 2000-02-08). However, this patent
involves using a special device for deinking of whole sheets and
cannot be used on pulp.
[0005] (ii) Another reference may be made to wherein, a
monocomponent cellulase is used for removal of ink and toners from
printed paper (U.S. Pat. No. 5,525,193 Neal, E., Steven, B. E.,
Hans, H. C. 1994. Use of monocomponent cellulase for removing inks,
coatings and toners from printed paper). The technique of isolating
monocomponent cellulase from microorganisms by various
purifications, chromatographic steps is time consuming and
expensive.
[0006] (iii) A reference may be made to a publication wherein,
endogluconases and hemicellulases from several fungi are used
effectively for deinking of xerox and laser-printed paper (Gubitz,
G. M., Mansfield, S. D., Bohm, D. Saddler, J. N. 1998. Effect of
endoglucanases and hemicellulases in magnetic and flotation
deinking of xerographic and laser-printed papers. Journal of
Biotechnology. 65:209-215). As mentioned above the enzyme
technology is still an expensive one for using on a large
scale.
[0007] (iv) A reference may be made to a publication, wherein
deinking of laser printed or xerographic paper is done using enzyme
containing a resistant binder. Enzyme is added in the specified
form to improve speck removal (Landley, J. G. Stockwell, J. O.
1997. Enzyme deinking of paper. Patent Number. GB 2304741 dated
26.03.97). As with the above references, the technology to produce
enzymes, their purification and application is an expensive
process.
[0008] (v) A reference may be made to a publication, wherein
deinking of office waste paper is carried out by contacting the
pulp with a deinking agent containing enzymes cellulase, preferably
active at pH 4 to 8. The treated paper is used in the production of
newsprint and high quality paper (Jobbins, J. M. Asbrand, Jr. G. R.
1996. Composition and process for deinking office waste paper.
Patent number: EP 717144 dated 19.06.96). As with the above
references, the technology to produce enzymes, their purification
and application is an expensive process.
[0009] (vi) A reference may be made to a publication, wherein
deinking of waste paper is achieved by incubating the paper pulp
with cellulase at 40.degree. C. for 1 hour under pressure.
Following dissociation of ink particles, the resulting washed pulp
is brighter (Mitsubishi Heavy Industries. 1994. Deinking of waste
paper. Patent number: JP06346390 dated 20.12.94). As with the above
references, the technology to produce enzymes, their purification
and application is an expensive process.
[0010] (vii) A reference may be made to a publication, wherein
deinking is achieved by using lipase to remove ink from used paper.
The procedure is carried out under neutral or alkaline conditions
which produces regenerated pulp having enhanced brightness (Novo.
1990. Removing ink from used paper. Patent number: JP2160984 dated
20.06.90).
[0011] (viii) A reference may be made to a publication, wherein
deinking of waste paper is done by using the yeast Hansenula
polymorpha, which degrades the ink-based resin (Jeonju-Paper. 1993.
Waste-paper deinking. Patent number: KR 9303705 dated 08.05.93).
However, the ink floats on the surface and the pulp has to be
extensively washed to remove the detached ink.
[0012] (ix) A reference may be made to a publication, wherein
decolorization of Indian ink is achieved by using thermostable
alkaline cellulase from a Bacillus sp (Kao. 1998. A
microbiologically produced thermostable alkaline cellulase and a
process for its preparation. Patent number: JP 10313859)
OBJECTS OF THE PRESENT INVENTION
[0013] The main object of the present invention is to provide a
process for decolorization of waste paper.
[0014] Another object of the present invention is to provide a
process for biological deinking of photocopied and inkjet-printed
paper pulp using a gram-negative coccoid bacterium deposited at
National Institute of Oceanography, Dona Paula, Goa 403 043, India
having accession No. NIO/DI/32 and being deposited at an
international depository ______ having accession number ______ and
also deposited at Microbial Technology, Chandigarh, India having
accession No. ______.
SUMMARY OF THE INVENTION
[0015] The present invention provides a process for decolorization
of paper pulp and more particularly, the present invention relates
to a process for biological deinking of photocopied and
inkjet-printed paper pulp using a gram negative coccoid bacteria
deposited at National Institute of Oceanography, Dona Paula, Goa
403 043, India having accession No. NIO/DI/32 and being deposited
at an international depository ______ having accession number
______ and also deposited at Microbial Technology, Chandigarh,
India having accession No. ______.
DETAILED DESCRIPTION OF THE PRESENT INVENTION
[0016] Accordingly, the present invention provides a process for
biological deinking of office waste paper inclusive of xerographic
and inkjet-printed paper printed with non-impact and
non-dispersible ink, said process comprises:
[0017] (a) growing coccoid bacterial isolate deposited at National
Institute of Oceanography, Dona Paula, Goa 403 043, India having
accession No. NIO/DI/32 and being deposited at an international
depository ______having accession number ______ and also deposited
at Microbial Technology, Chandigarh, India having accession No.
______, in a nutrient broth containing beef extract, peptic digest
of animal tissue and salinity ranging between 30 to 35 parts per
thousand for at least 4 days;
[0018] (b) adding the resulting biomass of step (a) at 20%
concentration to inoculate paper pulp diluted to at least 6%
consistency suspended in sea water with salinity diluted to
50%;
[0019] (c) incubating the inoculated pulp for a minimum period of
96 hours followed by diluting the pulp tenfold;
[0020] (d) adding 1% by wt. of a surfactant and aerating for at
least 10 minutes with compressed air to let the stray residual ink
particles and contaminants flow out of the container, and
[0021] (e) filtering the pulp of step (d) over a funnel under
suction, to get a sheet of pulp with uniform thickness and reading
the brightness by radiance measurement.
[0022] In an embodiment of the present invention, wherein in step
(a), the bacterium is grown for a minimum of 4 days in nutrient
broth containing beef extract and peptic digest of animal tissue in
sea water with salinity diluted to 50%.
[0023] In another embodiment of the present invention, wherein in
step (b), the resulting bacterial biomass is concentrated by
centrifugation and diluted to get final optical density of 1.0
measured at 660 nm in a spectrophotometer.
[0024] In yet another embodiment of the present invention, the
diluted bacterial culture is inoculated to at least 6% pulp
suspended in seawater with salinity diluted to 50% and incubated
for a minimum period of 4 days within to completely beach the
pulp.
[0025] In still another embodiment of the present invention, the
resulting bleached pulp is diluted tenfold, to which 1% surfactant
is added, aerated using compressed air, and any free residual ink
particles and contaminants are allowed to flow out of the
solution.
[0026] In a further embodiment of the present invention, wherein
after step (d) the xerographic or inkjet-printed paper is soaked in
hot water for 1-2 hours and macerated after adding 1% surfactant to
pulp.
[0027] In one more embodiment of the present invention, the paper
is macerated using conventional mixers.
[0028] In one another embodiment of the present invention, wherein
in step (e), the washed pulp is filtered over a Buchner funnel
under suction to get a pulp sheet of uniform thickness.
[0029] In an embodiment of the present invention, the pulp sheet is
pressed at 60.degree. C. for a minimum of 4-5 hours to make it
uniform and dried.
[0030] In another embodiment of the present invention, the radiance
of the resultant sheet is read from 412 to 684 nm and the radiance
expressed as Lux units (LU) {wherein one LU is equivalent to
.mu.W/cm.sup.2/nm/Sr}.
[0031] In still another embodiment of the present invention, the
pulp sheet made out of recycled paper is used as a reference.
[0032] In yet another embodiment of the present invention, wherein
in steps (a) and (b), pure water containing 1.5% by wt of sodium
chloride can be used in the place of sea water.
[0033] Deinking of paper is a limiting factor in recycling of
paper. Newsprint and office waste paper is generally deinked using
chemicals which go into the effluent and cause pollution of the
water bodies. The newsprint or offset printing is done using
dispersible or non-impact ink whereas laser, xerographic and
ink-jet printer ink does not disperse and is also called impact
ink. There are several patents describing various methods of
deinking by using hydrolytic enzymes such as cellulase,
hemicellulase and lipase of microbial origin which release toner
particles from fibers. However, enzyme action is affected by the
paper constituents in the deinking condition. The chemically pulped
fibers are more susceptible than mechanically pulped fibers. Office
waste paper is high in laser and toner content and the technology
for taking the toner particles out is not very good at the moment
(Jefferies, 2002).
[0034] In the present invention, the Applicants report a process
for deinking of office waste paper comprising xerographic and
ink-jet printed paper by a bacterium isolated from marine sediment
of an estuary in Goa, India. The salinity of the water at the time
of isolation was around 15 parts per thousand which is equivalent
to half-strength sea water.
[0035] The present invention provides a process for biological
deinking of office waste paper comprising xerographed, ink-jet
printed, and similar other non-impact ink printed papers. The
process is achieved by using a coccoid bacterium deposited National
Institute of Oceanography, Dona Paula, Goa 403 043, India having
accession No. NIO/DI/32 and being deposited at an international
depository ______ having accession number ______ and also deposited
at Microbial Technology, Chandigarh, India having accession No.
______, said culture may be grown in nutrient broth containing beef
extract, and peptic digest of animal tissue and prepared with sea
water with its salinity diluted to 50%.
[0036] The present invention more particularly provides a process
wherein the aforesaid pre-grown bacterial culture is inoculated
onto the office pulp suspended in seawater with its salinity
diluted to 50% and incubated for a minimum period of 4 days to
decolorize the paper pulp.
[0037] The paper pulp becomes brighter, the black ink is totally
decolorized and the supernatant water remains clear. The water
needs to be simply drained to recover the deinked pulp. The pulp
can be diluted tenfold Optionally to remove any free residual ink
particles and other contaminants. Conventional surfactant at 1%
concentration may be added to the diluted paper pulp, aerated for
at least 10 minutes by which the particles float and go out of the
pulp slurry. The washed pulp can be filtered over a large funnel
under suction and dried at 60.degree. C. for at least 6 hours to
obtain decolorized paper pulp.
[0038] The organism given in the present invention is a
gram-negative, coccoid bacterium isolated from coastal marine
sediment. The coccoid bacterium has been deposited at National
Institute of Oceanography, Dona Paula, Goa 403 043, India having
accession No. NIO/DI/32 and being deposited at an international
depository ______ having accession number ______ and also deposited
at Microbial Technology, Chandigarh, India having accession No.
______. Said bacterium can be grown in nutrient broth containing
beef extract, peptic digest of animal tissue, prepared with sea
water with salinity diluted to 50% and having a pH of at least 7.5.
The bacterium when grown in nutrient agar medium appears as
circular colonies off-white in colour, having 2 mm diameter
colonies in the beginning and growing to a size of 5 mm within 3-4
days. The bacterium is capable of producing lipase and amylase in
the presence of Tween 80 and starch respectively. It is
fermentative and catalase-negative bacterium. The bacterium is
grown at room temperature (30.degree. C.) for about 4 days, the
bacterial cells are collected by centrifugation under sterile
conditions, diluted appropriately to get optical density of 1 at
660 nm. Xerographic, ink-jet printed paper and other papers having
printed with impact ink are pulped by soaking in hot water for a
minimum of 2 hrs, macerated in a domestic mixer with surfactant
such as Tween 80. An example for the process for deinking involves
suspending such a pulp at least at 6% consistency in seawater with
salinity diluted to 50%, inoculating with 20% bacterial suspension
prepared as described above and incubating at room temperature for
a minimum of 4 days to get completely decolorized pulp and the ink
is disappeared from water also. The pulp can be diluted, aerated
from bottom to remove free contaminating particles, salts and
bacterial cells. A surfactant such as Tween 80 can be added at 1%
concentration for flotation of any residual ink particles. The pulp
is filtered over a Buchner funnel under suction, pressed with flat
stainless steel plates to make the pulp sheet uniform in thickness.
The radiance of the resultant sheet is read from 412 to 684 nm and
the radiance expressed in Lux units (Lu). One LU is equivalent to
.mu.W/cm.sup.2/nm/Sr. The ratio of radiance between recycled paper
purchased from the market and biologically deinked paper is taken
as a measure of brightness.
[0039] In a typical example plain toner powder of xerox machine
suspended in seawater with its salinity diluted to 50% also became
completely colorless within 7 days after inoculating it with the
said bacterial suspension.
[0040] Most of the organisms used for biological deinking release
ink particles from the fibers and they need to be subsequently
washed off from the pulp. In the process described in the present
invention, this problem does not arise as the ink and toner
particles are totally decolorized. The method is very cost
effective as the only step involved is raising the bacterial
inoculum in any conventional nutrient broth containing assimilable
carbon and nitrogen source. A comparative statement given in Table
1 explains the novelty of our invention in comparison with prior
art known in this field.
1TABLE 1 Comparative statement Type of Reference paper or Source of
(Prior art) ink deinking agent Remarks Patent Impact Chemical &
a surfactant, Not US6022423 ink and abrading the practical on
office paper and washing a large waste the deinked paper scale
using a special device to put whole sheet US5525193 Toner,
Monocomponent cellulase Enzyme impact purified from technology is
ink cellulolytic enzymes expensive from of various mixed
microorganisms office or genetically waste engineered orgainsm
which produces only monocomponent cellulase Gubitz et al. Xerox and
Endogluconases and Enzyme 1998 laser- hemicellulases technology is
printed from fungi expensive. paper GB2304741 Laser- Enzyme
containing Purification printed or a resistant and xerographic
binder application paper of enzyme technology is still expensive
Patent Office Cellulase Expensive EP 717144 waste enzyme paper
technology JP06346390 Office Contacting with Enzyme high waste
cellulase at temperature paper 40.degree. C. 1 h and pressure under
pressure will make the cost of technology not very practical.
JP2160984 Office Contacting Adjustment of waste pulp with pH and
enzyme paper lipase technology enzyme under will not neutral or
make it very alkaline practical. conditions KR9303705 Office
Contacting The ink floats waste pulp with the on the surface paper
culture of the and the pulp yeast Hansenula has to be polymorpha
extensively which degrades washed the ink- based resin. JP10313859
For Thermostable Recommen-ded decolor- alkaline for surfactant
ization on cellulase composition, as of Indian from a a fiber
treat- ink bacterium ment agent and as an Indian ink decolor-
ization agent. Our For Xerox Direct contact No enzyme, no invention
and inkjet- of pulp temp-erature or printed slurry with pH
adjustment, paper pulp the bacterium the pulp and NIO/DI/32 the
water are for 72 h to clear and get complete washing is
decolorization only optional. of the pulp and clear water
BRIEF DESCRIPTION OF ACCOMPANYING DRAWINGS
[0041] In the drawings accompanying the specification,
[0042] FIG. 1 shows the radiance of Xerox paper pulp (XP) is lowest
in comparison with that of recycled paper pulp (RP) and
biologically deinked pulp (BP);
[0043] FIG. 2 shows the ratio of radiance of biologically deinked
paper to that of recycled paper;
[0044] FIG. 3 compares the deinking done by the present invention
on papers of three different photocopier machines;
[0045] FIG. 4 compares the radiance of decolorized paper pulp after
4 days at different concentration of inoculum;
[0046] FIG. 5 compares the reflectance ratio of the deinked paper
pulp by at different concentrations of inoculum;
[0047] FIG. 6 compares the radiance ratio of the deinked paper pulp
with the number of days;
[0048] FIG. 7 shows the ratio of radiance between recycled paper
and deinked paper;
[0049] FIG. 8 shows that with an inoculam concentration of 2 ml,
maximum brightness is achieved after 72 hours and
[0050] FIG. 9 shows that with an inoculum concentration of 10 ml,
maximum brightness is achieved by 72 hours.
[0051] The present invention is further described with reference to
the accompanying examples, which are given by way of illustration
and therefore, should not be construed to limit the scope of the
present invention in any manner.
EXAMPLE 1
[0052] The bacterial isolate NIO/DI/32 was isolated from the
coastal esturine sediment, Dona Paula, Goa, India in nutrient agar
medium containing beef extract and peptic digest of animal tissue
and agar in sea water with its salinity diluted to 50%. The culture
was maintained in the slants of nutrient agar medium for all
further experiments.
[0053] The said bacterium can be grown in nutrient broth containing
beef extract, peptic digest of animal tissue in sea water with its
salinity diluted to 50%. The bacterium is grown at room temperature
(30.degree. C.) for about 4 days, the bacterial cells are collected
by centrifugation under sterile conditions, diluted appropriately
to get optical density of 1 at 660 nm using a spectrophotometer.
Xerographic paper printed with impact ink from HP machine are
pulped by soaking in hot water for a minimum of 2 hrs, macerated in
a conventional domestic mixer with surfactant such as Tween 80. An
example for the process for deinking involves suspending such a
pulp at least at 6% consistency (6 g wet pulp in 100 ml of water)
in sea water with its salinity diluted to 50%, inoculating with 20%
bacterial suspension prepared as described above and incubating at
room temperature for a minimum of 4 days to get completely bleached
pulp with the ink totally disappearing from the supernatant water.
The pulp can be diluted, aerated from bottom to remove free
contaminating particles, salts and bacterial cells. A surfactant
such as Tween 80 can be added at 1% concentration for flotation of
any residual ink particles. The pulp is filtered over a Buchner
funnel under suction, pressed with flat stainless steel plates to
make the pulp sheet uniform in thickness. The radiance of the
resultant sheet is read from 412 to 684 nm and the radiance
expressed in Lux units (Lu). One LU is equivalent to
.mu.W/cm.sup.2/nm/Sr. The ratio of radiance between recycled paper
available in any stationery shop and biologically deinked paper is
taken as a measure of brightness.
[0054] Accordingly, FIG. 1 shows the radiance of Xerox paper pulp
(XP) is lowest in comparison with that of recycled paper pulp (RP)
and biologically deinked pulp (BP). This brightness was achieved 4
days after inoculation with the said bacterium. As the target was
to achieve brightness of recycled paper, the Applicants have shown
the same data as ratio of radiance of Xerox paper to recycled paper
and similarly, the ratio of radiance of biologically deinked paper
to that of recycled paper is shown in FIG. 2.
[0055] Xerographic printed paper obtained from various Xerox
machines were compared for their efficiency in being deinked by the
said bacterium. Accordingly FIG. 3 shows that out of 3 different
machines, Xerox paper obtained from Lanier machine were deinked
effectively by the process described in the present invention.
EXAMPLE 2
[0056] As seen in the previous example, the bacterium could grow in
the plain paper pulp suspended in water, and bleach the pulp in 4
days, the Xerox toner (HP make) at 1% concentration was suspended
in sterile sea water and inoculated with the said bacterial
suspension and incubated at room temperature for 15 days. The
control flask did not receive any bacterial inoculum. The contents
of experimental flask and the control flask were filtered over
pre-weighed filter paper, rinsed with sterile distilled water to
remove salts and dried to a constant weight at 60.degree. C. The
difference in weight of residual toner powder was compared in the
two sets.
[0057] Accordingly, in the experimental flask after 15 days the dry
weight was 143 mg whereas, in the control flask it was 220 mg.
Thus, almost 65% toner was degraded in the inoculated flasks.
Besides, the water in the experimental flask became almost
colorless.
EXAMPLE 3
[0058] The ability of the bacterial isolate having accession number
NIO/DI/32 (also being deposited at an international depository) to
deink ink-jet printed paper was tried as follows:
[0059] The inkjet printed-paper was prepared into pulp as described
in Example 1 and inoculated with the said bacterium as described in
Example 1. However, the inoculum dose varied from 2 ml to 10 ml
with the increment of 2 ml. The other procedures used were the same
as described n the Example 1.
[0060] Accordingly, FIG. 4 shows radiance of decolorized paper pulp
after 4 days in comparison with Xerox and recycled paper. The
radiance of biologically deinked paper was more than the recycled
paper and there was not much difference between inoculum
concentration of 6 to 10 ml in the final radiance achieved.
[0061] FIG. 5 shows the ratio of reflectance between Xerox paper to
recycled paper in comparison with paper pulp deinked using
different concentrations of the said bacterial suspension. The
brightness achieved by using 8 ml or 10 ml inoculum was almost
similar.
EXAMPLE 4
[0062] The deinking of inkjet-printed paper in relation to time of
incubation (in days) was carried out as follows:
[0063] The preparation of pulp, inoculum and experimental set up
were same as in the previous example, but the inoculum
concentration used here was 10 ml and radiance of the pulp was
tested on day 2, 4, 6 and 8 days after incubating the pulp with the
said bacterial culture.
[0064] Accordingly, FIG. 6 shows that by day 6 maximum brightness
is achieved and here also the radiance of biologically deinked pulp
was slightly greater than that of recycled paper purchased from the
market. The control was the inkjet printed paper pulp.
[0065] FIG. 7 shows the ratio of radiance between recycled paper
and deinked paper. The control was the ink-jet printed paper pulp
which showed lowest radiance ratio.
EXAMPLE 5
[0066] Ability of the said bacterium to deink the inkjet--printed
paper with two concentrations of the inoculum at varying incubation
period was compared to arrive at a combination where deinking is
achieved in a shortest time.
[0067] Accordingly FIG. 8 shows that with an inoculam concentration
of 2 ml, maximum brightness is achieved after 72 hours.
[0068] FIG. 9 also shows that with an inoculum concentration of 10
ml, maximum brightness is achieved by 72 hours.
* * * * *
References