U.S. patent application number 10/404944 was filed with the patent office on 2003-09-18 for open circle probes with intramolecular stem structures.
Invention is credited to Abarzua, Patricio, Alsmadi, Osama A..
Application Number | 20030175788 10/404944 |
Document ID | / |
Family ID | 25187247 |
Filed Date | 2003-09-18 |
United States Patent
Application |
20030175788 |
Kind Code |
A1 |
Alsmadi, Osama A. ; et
al. |
September 18, 2003 |
Open circle probes with intramolecular stem structures
Abstract
Disclosed are compositions and methods for reducing or
eliminating generation of unwanted, undesirable, or non-specific
amplification products in nucleic acid amplification reactions,
such as rolling circle amplification. One form of composition is an
open circle probe that can form an intramolecular stem structure,
such as a hairpin structure, at one or both ends. The stem
structure allows the open circle probe to be circularized when
hybridized to a legitimate target sequence but results in
inactivation of uncircularized open circle probes. This
inactivation, which preferably involves stabilization of the stem
structure, extension of the end of the open circle probe, or both,
reduces or eliminates the ability of the open circle probe to prime
nucleic acid synthesis or to serve as a template for rolling circle
amplification. The disclosed method is useful for detection,
quantitation, and/or location of any desired analyte, such as
proteins and peptides.
Inventors: |
Alsmadi, Osama A.; (Hamden,
CT) ; Abarzua, Patricio; (West Caldwell, NJ) |
Correspondence
Address: |
NEEDLE & ROSENBERG, P.C.
SUITE 1000
999 PEACHTREE STREET
ATLANTA
GA
30309-3915
US
|
Family ID: |
25187247 |
Appl. No.: |
10/404944 |
Filed: |
March 31, 2003 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
10404944 |
Mar 31, 2003 |
|
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09803713 |
Mar 9, 2001 |
|
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6573051 |
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Current U.S.
Class: |
435/6.12 ;
435/91.2 |
Current CPC
Class: |
C12Q 1/6844 20130101;
C12Q 1/6844 20130101; C12Q 2525/307 20130101; C12Q 2525/301
20130101; C12Q 2531/125 20130101; C12Q 2525/301 20130101 |
Class at
Publication: |
435/6 ;
435/91.2 |
International
Class: |
C12Q 001/68; C12P
019/34 |
Claims
I claim:
1. A method of amplifying nucleic acid sequences, the method
comprising a DNA ligation operation and an amplification operation,
wherein the DNA ligation operation comprises circularization of one
or more open circle probes, wherein each open circle probe
comprises two ends, wherein at least one of the ends of at least
one of the open circle probes can form an intramolecular stem
structure, wherein circularization of the open circle probes that
can form an intramolecular stem structure is dependent on
hybridization of the open circle probe to a target sequence,
wherein the amplification operation comprises rolling circle
replication of the circularized open circle probes.
2. The method of claim 1 wherein if one or more of the open circle
probes that can form an intramolecular stem structure are not
circularized, the end of at least one of the uncircularized open
circle probes that forms the intramolecular stem structure is
extended during the amplification operation using the open circle
probe as a template.
3. The method of claim 2 wherein extension of the end of the open
circle probes prevents the extended open circle probes from serving
as a template for rolling circle replication.
4. The method of claim 2 wherein extension of the end of the open
circle probes stabilizes the intramolecular stem structure, wherein
the stabilized intramolecular stem structure prevents the extended
open circle probes from priming nucleic acid replication.
5. The method of claim 1 wherein the intramolecular stem structure
can form under the conditions used for the amplification
operation.
6. The method of claim 5 wherein the intramolecular stem structure
prevents the open circle probes from priming nucleic acid
replication.
7. The method of claim 5 wherein the intramolecular stem structure
prevents the open circle probes from serving as a template for
rolling circle replication.
8. The method of claim 1 wherein the intramolecular stem structure
prevents the open circle probes from priming nucleic acid
replication.
9. The method of claim 1 wherein the intramolecular stem structure
prevents the open circle probes from serving as a template for
rolling circle replication.
10. The method of claim 1 wherein the intramolecular stem structure
forms a hairpin structure.
11. The method of claim 1 wherein the intramolecular stem structure
forms a stem and loop structure.
12. The method of claim 11 wherein the two ends of the open circle
probe together form the intramolecular stem structure.
13. The method of claim 1 wherein one of the ends of the open
circle probes is a 3' end, wherein the 3' end of at least one of
the open circle probes can form an intramolecular stem
structure.
14. The method of claim 13 wherein the other end of the open circle
probes is a 5' end, wherein the 5' end of at least one of the open
circle probes can form an intramolecular stem structure.
15. The method of claim 12 wherein one of the ends of the open
circle probes is a 5' end, wherein the 5' end of at least one of
the open circle probes can form an intramolecular stem
structure.
16. The method of claim 1 wherein the open circle probes are each
specific for a target sequence, wherein each target sequence
comprises a 5' region and a 3' region, wherein each open circle
probe comprises a single-stranded, linear DNA molecule, wherein the
single-stranded, linear DNA molecule comprises, from 5' end to 3'
end, a 5' phosphate group, a right target probe portion, a spacer
portion, a left target probe portion, and a 3' hydroxyl group,
wherein the left target probe portion is complementary to the 3'
region of the target sequence, wherein the right target probe
portion is complementary to the 5' region of the target
sequence.
17. The method of claim 16 wherein the intramolecular stem
structure of at least one of the open circle probes forms a stem
and loop structure.
18. The method of claim 17 wherein a portion of one of the target
probe portions of at least one of the open circle probes is in the
loop of the stem and loop structure, wherein the portion of the
target probe portion in the loop can hybridize to the target
sequence, wherein hybridization of the target probe portion in the
loop to the target sequence disrupts the intramolecular stem
structure.
19. The method of claim 18 wherein disruption of the intramolecular
stem structure allows the end of the open circle probes that can
form an intramolecular stem structure to hybridize to the target
sequence.
20. The method of claim 19 wherein a hybrid between the target
sequence and the target probe portion at the end of the open circle
probes that can form an intramolecular stem structure is more
stable than the intramolecular stem structure.
21. The method of claim 18 wherein hybridization of the loop to a
sequence other than the target sequence does not disrupt the
intramolecular stem structure.
22. The method of claim 16 wherein a hybrid between the target
sequence and the target probe portion at the end of the open circle
probes that can form an intramolecular stem structure is more
stable than the intramolecular stem structure.
23. The method of claim 16 wherein the spacer portion comprises a
primer complement portion.
24. The method of claim 16 wherein at least one of the target
sequences further comprises a central region located between the 5'
region and the 3' region, wherein neither the left target probe
portion of the open circle probe specific for the target sequence
nor the right target probe portion of the open circle probe
specific for the target sequence is complementary to the central
region of the target sequence.
25. The method of claim 24 wherein the ligation operation comprises
mixing the open circle probes and one or more gap oligonucleotides
with one or more target samples, and incubating under conditions
that promote hybridization between the open circle probes and the
gap oligonucleotides and the target sequences, and ligation of the
open circle probes and gap oligonucleotides to form the
circularized open circle probes, wherein each gap oligonucleotide
comprises a single-stranded, linear DNA molecule comprising a 5'
phosphate group and a 3' hydroxyl group, wherein each gap
oligonucleotide is complementary all or a portion of the central
region of the target sequence.
26. The method of claim 24 wherein a complement to the central
region of the target sequence is synthesized during the ligation
operation.
27. The method of claim 16 wherein a plurality of the open circle
probes are each specific for a different target sequence.
28. The method of claim 27 wherein a plurality of different target
sequences are detected.
29. The method of claim 27 wherein the amplification operation
produces amplified nucleic acid, the method further comprising
detecting the amplified nucleic acid with one or more detection
probes.
30. The method of claim 29 wherein a portion of each of a plurality
of the detection probes has sequence matching or complementary to a
portion of a different one of the open circle probes, wherein a
plurality of different amplified nucleic acids are detected using
the plurality of detection probes.
31. The method of claim 1 wherein at least one of the target
sequences is coupled to a specific binding molecule.
32. The method of claim 31 wherein the method further comprises
bringing into contact the specific binding molecule and a target
molecule, wherein the specific binding molecule binds to the target
molecule.
33. The method of claim 32 wherein the specific binding molecule
and target molecule are brought into contact prior to the ligation
operation.
34. The method of claim 32 wherein the specific binding molecule
and target molecule are brought into contact following the ligation
operation.
35. The method of claim 32 wherein the specific binding molecule
and target molecule are brought into contact following the
amplification operation.
36. The method of claim 32 wherein the target molecule is a
peptide, protein, carbohydrate, lipid, nucleic acid, or
metabolite.
37. The method of claim 32 wherein the target molecule is present
in, or derived from, tissue, bodily fluid, or cells.
38. The method of claim 32 wherein the target molecule is present
in, or derived from, tissue.
39. The method of claim 31 wherein the specific binding molecule is
an antibody.
40. The method of claim 31 wherein a plurality of different target
sequences are coupled to specific binding molecules.
41. The method of claim 40 wherein at least one of the specific
binding molecules is specific for a protein or peptide.
42. The method of claim 41 wherein a plurality of specific binding
molecules are each specific for a different protein or peptide.
43. The method of claim 40 wherein a plurality of the specific
binding molecules are each specific for a different analyte.
44. The method of claim 40 wherein a plurality of the specific
binding molecules are each specific for a different target
molecule, wherein the open circle probes are each specific for a
different target sequence.
45. The method of claim 44 further comprising bringing into contact
the specific binding molecules and one or more target molecules,
wherein the specific binding molecule binds to the target molecule,
wherein a plurality of different target molecules are detected via
rolling circle replication of circularized open circle probes
specific for the target sequences coupled to the specific binding
molecules that are specific for the target molecules.
46. The method of claim 45 wherein a plurality of different target
molecules are detected in the same assay.
47. The method of claim 1 wherein rolling circle replication is
primed by a rolling circle replication primer, wherein the rolling
circle replication primer is coupled to a specific binding
molecule, wherein the specific binding molecule is bound to a
target molecule.
48. The method of claim 1 wherein the amplification operation
produces tandem sequence DNA, wherein the method further comprises
detecting the tandem sequence DNA.
49. The method of claim 1 wherein the amplification operation
produces tandem sequence DNA and secondary tandem sequence DNA,
wherein the method further comprises detecting the tandem sequence
DNA, the secondary tandem sequence DNA, or both.
50. The method of claim 1 wherein rolling circle replication is
primed by one or more rolling circle replication primers, wherein
each rolling circle replication primer comprises two ends, wherein
at least one of the ends of at least one of the rolling circle
replication primers can form an intramolecular stem structure,
wherein priming by the rolling circle replication primers that can
form an intramolecular stem structure is dependent on hybridization
of the rolling circle replication primers to the amplification
target circles.
51. The method of claim 50 wherein both ends of at least one of the
rolling circle replication primers can form an intramolecular stem
structure.
52. The method of claim 51 wherein the two ends of at least one of
the rolling circle replication primers are hybridized to each
other.
53. The method of claim 51 wherein the two ends of at least one of
the rolling circle replication primers are a 3' end and a 5' end,
wherein the 3' end and 5' end are both involved in the
intramolecular stem structure such that the 3' end has a short
unpaired overhang when the intramolecular stem structure is
formed.
54. The method of claim 50 wherein the intramolecular stem
structure of at least one of the rolling circle replication primers
forms a stem and loop structure.
55. The method of claim 54 wherein each amplification target circle
comprises a primer complement portion, wherein each rolling circle
replication primer has a complement portion, wherein the complement
portion of each rolling circle replication primer is complementary
to the primer complement portion of at least one of the
amplification target circles, wherein a portion of the complement
portion of at least one of the rolling circle replication primers
is in the loop of the stem and loop structure, wherein the portion
of the complement portion in the loop can hybridize to the primer
complement portion of at least one of the amplification target
circles, wherein hybridization of the complement portion in the
loop to the primer complement portion disrupts the intramolecular
stem structure.
56. The method of claim 55 wherein disruption of the intramolecular
stem structure allows the end of the rolling circle replication
primers that can form an intramolecular stem structure to hybridize
to the primer complement portion of the amplification target
circle.
57. The method of claim 56 wherein a hybrid between the primer
complement portion of the amplification target circle and the
complement portion of the rolling circle replication primers that
can form an intramolecular stem structure is more stable than the
intramolecular stem structure.
58. The method of claim 55 wherein hybridization of the loop to a
sequence other than the primer complement portion of the
amplification target circle does not disrupt the intramolecular
stem structure.
59. The method of claim 1 wherein the amplification operation
produces tandem sequence DNA, wherein the amplification operation
further comprises secondary DNA strand displacement.
60. The method of claim 59 wherein secondary DNA strand
displacement is primed by one or more secondary DNA strand
displacement primers, wherein each secondary DNA strand
displacement primer comprises two ends, wherein at least one of the
ends of at least one of the secondary DNA strand displacement
primers can form an intramolecular stem structure, wherein priming
by the secondary DNA strand displacement primers that can form an
intramolecular stem structure is dependent on hybridization of the
secondary DNA strand displacement primers to the tandem sequence
DNA.
61. The method of claim 60 wherein the secondary DNA strand
displacement primers prime secondary DNA strand displacement by
hybridizing to the tandem sequence DNA, wherein both ends of at
least one of the secondary DNA strand displacement primers can form
an intramolecular stem structure.
62. The method of claim 61 wherein the two ends of at least one of
the secondary DNA strand displacement primers are hybridized to
each other.
63. The method of claim 61 wherein the two ends of at least one of
the secondary DNA strand displacement primers are a 3' end and a 5'
end, wherein the 3' end and 5' end are both involved in the
intramolecular stem structure such that the 3' end has a short
unpaired overhang when the intramolecular stem structure is
formed.
64. The method of claim 60 wherein the intramolecular stem
structure of at least one of the secondary DNA strand displacement
primers forms a stem and loop structure.
65. The method of claim 64 wherein each secondary DNA strand
displacement primer has a matching portion, wherein the matching
portion of each secondary DNA strand displacement primer is
complementary to the tandem sequence DNA, wherein a portion of the
matching portion of at least one of the secondary DNA strand
displacement primers is in the loop of the stem and loop structure,
wherein the portion of the matching portion in the loop can
hybridize to the tandem sequence DNA, wherein hybridization of the
matching portion in the loop to the tandem sequence DNA disrupts
the intramolecular stem structure.
66. The method of claim 65 wherein disruption of the intramolecular
stem structure allows the end of the secondary DNA strand
displacement primers that can form an intramolecular stem structure
to hybridize to the tandem sequence DNA.
67. The method of claim 66 wherein a hybrid between the tandem
sequence DNA and the matching portion of the secondary DNA strand
displacement primers that can form an intramolecular stem structure
is more stable than the intramolecular stem structure.
68. The method of claim 65 wherein hybridization of the loop to a
sequence other than sequence in the tandem sequence DNA does not
disrupt the intramolecular stem structure.
69. A method of selectively amplifying nucleic acid sequences
related to one or more target sequences, the method comprising, (a)
mixing one or more different open circle probes with a target
sample, to produce an OCP-target sample mixture, and incubating the
OCP-target sample mixture under conditions that promote
hybridization between the open circle probes and the target
sequences in the OCP-target sample mixture, (b) mixing ligase with
the OCP-target sample mixture, to produce a ligation mixture, and
incubating the ligation mixture under conditions that promote
ligation of the open circle probes to form amplification target
circles, (c) mixing a rolling circle replication primer with the
ligation mixture, to produce a primer-ATC mixture, and incubating
the primer-ATC mixture under conditions that promote hybridization
between the amplification target circles and the rolling circle
replication primer in the primer-ATC mixture, and (d) mixing DNA
polymerase with the primer-ATC mixture, to produce a polymerase-ATC
mixture, and incubating the polymerase-ATC mixture under conditions
that promote replication of the amplification target circles,
wherein replication of the amplification target circles results in
the formation of tandem sequence DNA; wherein at least one of the
open circle probes comprises two ends, wherein at least one of the
ends of the open circle probe can form an intramolecular stem
structure.
70. The method of claim 69 wherein if the open circle probe that
can form an intramolecular stem structure is not ligated to form an
amplification target circle the end of the open circle probe is
extended during replication of the amplification target circles
using the open circle probe as a template.
71. The method of claim 70 wherein extension of the end of the open
circle probe prevents the extended open circle probes from serving
as a template for rolling circle replication.
72. The method of claim 70 wherein extension of the end of the open
circle probe stabilizes the intramolecular stem structure, wherein
the stabilized intramolecular stem structure prevents the extended
open circle probes from priming nucleic acid replication.
73. The method of claim 69 wherein the intramolecular stem
structure can form under the conditions used for replication of the
amplification target circles.
74. The method of 73 wherein the intramolecular stem structure
prevents the open circle probe that can form an intramolecular stem
structure from priming nucleic acid replication.
75. The method of 73 wherein the intramolecular stem structure
prevents the open circle probe that can form an intramolecular stem
structure from serving as a template for rolling circle
replication.
76. The method of 69 wherein the intramolecular stem structure
prevents the open circle probe that can form an intramolecular stem
structure from priming nucleic acid replication.
77. The method of 69 wherein the intramolecular stem structure
prevents the open circle probe that can form an intramolecular stem
structure from serving as a template for rolling circle
replication.
78. The method of claim 69 wherein the intramolecular stem
structure forms a hairpin structure.
79. The method of claim 69 wherein the intramolecular stem
structure forms a stem and loop structure.
80. The method of claim 79 wherein the two ends of the open circle
probe that can form an intramolecular stem structure together form
the intramolecular stem structure.
81. The method of claim 69 wherein one of the ends of the open
circle probe that can form an intramolecular stem structure is a 3'
end, wherein the 3' end of the open circle probe can form an
intramolecular stem structure.
82. The method of claim 81 wherein the other end of the open circle
probe is a 5' end, wherein the 5' end of the open circle probe can
form an intramolecular stem structure.
83. The method of claim 80 wherein one of the ends of the open
circle probe is a 5' end, wherein the 5' end of the open circle
probe can form an intramolecular stem structure.
84. The method of claim 69 wherein the target sequence to which the
open circle probe that can form an intramolecular stem structure
can hybridize comprises a 5' region and a 3' region, wherein the
open circle probe comprises a single-stranded, linear DNA molecule,
wherein the single-stranded, linear DNA molecule comprises, from 5'
end to 3' end, a 5' phosphate group, a right target probe portion,
a spacer portion, a left target probe portion, and a 3' hydroxyl
group, wherein the left target probe portion is complementary to
the 3' region of the target sequence, wherein the right target
probe portion is complementary to the 5' region of the target
sequence.
85. The method of claim 84 wherein the intramolecular stem
structure forms a stem and loop structure.
86. The method of claim 85 wherein a portion of one of the target
probe portions is in the loop of the stem and loop structure,
wherein the portion of the target probe portion in the loop can
hybridize to the target sequence, wherein hybridization of the
target probe portion in the loop to the target sequence disrupts
the intramolecular stem structure.
87. The method of claim 86 wherein disruption of the intramolecular
stem structure allows the end of the open circle probe that can
form an intramolecular stem structure to hybridize to the target
sequence.
88. The method of claim 87 wherein a hybrid between the target
sequence and the target probe portion at the end of the open circle
probe that can form an intramolecular stem structure is more stable
than the intramolecular stem structure.
89. The method of claim 84 wherein a hybrid between the target
sequence and the target probe portion at the end of the open circle
probe that can form an intramolecular stem structure is more stable
than the intramolecular stem structure.
90. The method of claim 84 wherein the spacer portion comprises a
primer complement portion.
91. The method of claim 84 wherein the target sequence further
comprises a central region located between the 5' region and the 3'
region, wherein neither the left target probe portion of the open
circle probe nor the right target probe portion of the open circle
probe is complementary to the central region of the target
sequence.
92. The method of claim 91 wherein step (a) further comprises
mixing one or more gap oligonucleotides with the target sample,
wherein each gap oligonucleotide comprises a single-stranded,
linear DNA molecule comprising a 5' phosphate group and a 3'
hydroxyl group, wherein each gap oligonucleotide is complementary
all or a portion of the central region of the target sequence.
93. The method of claim 91 wherein a complement to the central
region of the target sequence is synthesized during step (b).
94. The method of claim 69 wherein at least one of the target
sequences is coupled to a specific binding molecule.
95. The method of claim 94 wherein the method further comprises
bringing into contact the specific binding molecule and a target
molecule, wherein the specific binding molecule binds to a target
molecule.
96. The method of claim 95 wherein the specific binding molecule
and target molecule are brought into contact prior to step (a).
97. The method of claim 95 wherein the specific binding molecule
and target molecule are brought into contact following step(b).
98. The method of claim 95 wherein the specific binding molecule
and target molecule are brought into contact following step
(d).
99. The method of claim 95 wherein the target molecule is a
peptide, protein, carbohydrate, lipid, nucleic acid, or
metabolite.
100. The method of claim 95 wherein the target molecule is present
in, or derived from, tissue, bodily fluid, or cells.
101. The method of claim 95 wherein the target molecule is present
in, or derived from, tissue.
102. The method of claim 94 wherein the specific binding molecule
is an antibody.
103. The method of claim 94 wherein the rolling circle replication
primer is coupled to a specific binding molecule, wherein the
specific binding molecule is bound to a target molecule.
104. The method of claim 69 wherein the method further comprises
detecting the tandem sequence DNA.
105. The method of claim 69 wherein the tandem sequence DNA is
replicated to form secondary tandem sequence DNA, wherein the
method further comprises detecting the tandem sequence DNA, the
secondary tandem sequence DNA, or both.
106. A method of amplifying nucleic acid sequences, the method
comprising an amplification operation, wherein the amplification
operation comprises rolling circle replication of one or more
amplification target circles, wherein rolling circle replication is
primed by one or more rolling circle replication primers, wherein
each rolling circle replication primer comprises two ends, wherein
at least one of the ends of at least one of the rolling circle
replication primers can form an intramolecular stem structure,
wherein priming by the rolling circle replication primers that can
form an intramolecular stem structure is dependent on hybridization
of the rolling circle replication primers to the amplification
target circles.
107. The method of claim 106 wherein both ends of at least one of
the rolling circle replication primers can form an intramolecular
stem structure.
108. The method of claim 107 wherein the two ends of at least one
of the rolling circle replication primers are hybridized to each
other.
109. The method of claim 107 wherein the two ends of at least one
of the rolling circle replication primers are a 3' end and a 5'
end, wherein the 3' end and 5' end are both involved in the
intramolecular stem structure such that the 3' end has a short
unpaired overhang when the intramolecular stem structure is
formed.
110. The method of claim 106 wherein the intramolecular stem
structure of at least one of the rolling circle replication primers
forms a stem and loop structure.
111. The method of claim 110 wherein each amplification target
circle comprises a primer complement portion, wherein each rolling
circle replication primer has a complement portion, wherein the
complement portion of each rolling circle replication primer is
complementary to the primer complement portion of at least one of
the amplification target circles, wherein a portion of the
complement portion of at least one of the rolling circle
replication primers is in the loop of the stem and loop structure,
wherein the portion of the complement portion in the loop can
hybridize to the primer complement portion of at least one of the
amplification target circles, wherein hybridization of the
complement portion in the loop to the primer complement portion
disrupts the intramolecular stem structure.
112. The method of claim 111 wherein disruption of the
intramolecular stem structure allows the end of the rolling circle
replication primers that can form an intramolecular stem structure
to hybridize to the primer complement portion of the amplification
target circle.
113. The method of claim 112 wherein a hybrid between the primer
complement portion of the amplification target circle and the
complement portion of the rolling circle replication primers that
can form an intramolecular stem structure is more stable than the
intramolecular stem structure.
114. The method of claim 111 wherein hybridization of the loop to a
sequence other than the primer complement portion of the
amplification target circle does not disrupt the intramolecular
stem structure.
115. A method of amplifying nucleic acid sequences, the method
comprising an amplification operation, wherein the amplification
operation comprises rolling circle replication of one or more
amplification target circles, wherein the amplification operation
produces tandem sequence DNA, wherein the amplification operation
further comprises secondary DNA strand displacement.
116. The method of claim 115 wherein secondary DNA strand
displacement is primed by one or more secondary DNA strand
displacement primers, wherein each secondary DNA strand
displacement primer comprises two ends, wherein at least one of the
ends of at least one of the secondary DNA strand displacement
primers can form an intramolecular stem structure, wherein priming
by the secondary DNA strand displacement primers that can form an
intramolecular stem structure is dependent on hybridization of the
secondary DNA strand displacement primers to the tandem sequence
DNA.
117. The method of claim 116 wherein the secondary DNA strand
displacement primers prime secondary DNA strand displacement by
hybridizing to the tandem sequence DNA, wherein both ends of at
least one of the secondary DNA strand displacement primers can form
an intramolecular stem structure.
118. The method of claim 117 wherein the two ends of at least one
of the secondary DNA strand displacement primers are hybridized to
each other.
119. The method of claim 117 wherein the two ends of at least one
of the secondary DNA strand displacement primers are a 3' end and a
5' end, wherein the 3' end and 5' end are both involved in the
intramolecular stem structure such that the 3' end has a short
unpaired overhang when the intramolecular stem structure is
formed.
120. The method of claim 116 wherein the intramolecular stem
structure of at least one of the secondary DNA strand displacement
primers forms a stem and loop structure.
121. The method of claim 120 wherein each secondary DNA strand
displacement primer has a matching portion, wherein the matching
portion of each secondary DNA strand displacement primer is
complementary to the tandem sequence DNA, wherein a portion of the
matching portion of at least one of the secondary DNA strand
displacement primers is in the loop of the stem and loop structure,
wherein the portion of the matching portion in the loop can
hybridize to the tandem sequence DNA, wherein hybridization of the
matching portion in the loop to the tandem sequence DNA disrupts
the intramolecular stem structure.
122. The method of claim 121 wherein disruption of the
intramolecular stem structure allows the end of the secondary DNA
strand displacement primers that can form an intramolecular stem
structure to hybridize to the tandem sequence DNA.
123. The method of claim 122 wherein a hybrid between the tandem
sequence DNA and the matching portion of the secondary DNA strand
displacement primers that can form an intramolecular stem structure
is more stable than the intramolecular stem structure.
124. The method of claim 121 wherein hybridization of the loop to a
sequence other than sequence in the tandem sequence DNA does not
disrupt the intramolecular stem structure.
125. An open circle probe wherein the open circle probe comprises
two ends, wherein at least one of the ends of the open circle probe
can form an intramolecular stem structure.
126. The open circle probe of claim 125 wherein the intramolecular
stem structure forms a hairpin structure.
127. The open circle probe of claim 125 wherein the intramolecular
stem structure forms a stem and loop structure.
128. The open circle probe of claim 127 wherein the two ends of the
open circle probe together form the intramolecular stem
structure.
129. The open circle probe of claim 125 wherein one of the ends of
the open circle probe is a 3' end, wherein the 3' end of the open
circle probe can form an intramolecular stem structure.
130. The open circle probe of claim 129 wherein the other end of
the open circle probe is a 5' end, wherein the 5' end of the open
circle probe can form an intramolecular stem structure.
131. The open circle probe of claim 125 wherein one of the ends of
the open circle probe is a 5' end, wherein the 5' end of the open
circle probe can form an intramolecular stem structure.
132. The open circle probe of claim 125 wherein the intramolecular
stem structure can form under conditions suitable for nucleic acid
replication.
133. The open circle probe of 132 wherein the intramolecular stem
structure prevents the open circle probe from priming nucleic acid
replication.
134. The open circle probe of 132 wherein the intramolecular stem
structure prevents the open circle probe from serving as a template
for rolling circle replication.
135. The open circle probe of 125 wherein the intramolecular stem
structure prevents the open circle probe from priming nucleic acid
replication.
136. The open circle probe of 125 wherein the intramolecular stem
structure prevents the open circle probe from serving as a template
for rolling circle replication.
137. The open circle probe of claim 125 wherein the open circle
probe is specific for a target sequence, wherein the target
sequence comprises a 5' region and a 3' region, wherein the open
circle probe comprises a single-stranded, linear DNA molecule,
wherein the single-stranded, linear DNA molecule comprises, from 5'
end to 3' end, a 5' phosphate group, a right target probe portion,
a spacer portion, a left target probe portion, and a 3' hydroxyl
group, wherein the left target probe portion is complementary to
the 3' region of the target sequence, wherein the right target
probe portion is complementary to the 5' region of the target
sequence.
138. The open circle probe of claim 137 wherein the intramolecular
stem structure forms a stem and loop structure.
139. The open circle probe of claim 138 wherein a portion of one of
the target probe portions is in the loop of the stem and loop
structure, wherein the portion of the target probe portion in the
loop can hybridize to the target sequence, wherein hybridization of
the target probe portion in the loop to the target sequence
disrupts the intramolecular stem structure.
140. The open circle probe of claim 139 wherein disruption of the
intramolecular stem structure allows the end of the open circle
probe that can form an intramolecular stem structure to hybridize
to the target sequence.
141. The open circle probe of claim 140 wherein a hybrid between
the target sequence and the target probe portion at the end of the
open circle probe that can form an intramolecular stem structure is
more stable than the intramolecular stem structure.
142. The open circle probe of claim 137 wherein a hybrid between
the target sequence and the target probe portion at the end of the
open circle probe that can form an intramolecular stem structure is
more stable than the intramolecular stem structure.
143. The open circle probe of claim 137 wherein the spacer portion
comprises a primer complement portion.
144. The open circle probe of claim 137 wherein the target sequence
further comprises a central region located between the 5' region
and the 3' region, wherein neither the left target probe portion of
the open circle probe nor the right target probe portion of the
open circle probe is complementary to the central region of the
target sequence.
145. A kit for selectively detecting one or more target sequences
or selectively amplifying nucleic acid sequences related to one or
more target sequences, the kit comprising, one or more open circle
probes each comprising two ends, wherein at least one of the ends
of one of the open circle probe can form an intramolecular stem
structure, wherein portions of each open circle probe are
complementary to the one or more target sequences, and one or more
rolling circle replication primers, wherein all or a portion of
each rolling circle replication primer is complementary to a
portion of one or more of the open circle probes.
146. The kit of claim 145 wherein the end of the open circle probe
that can form an intramolecular stem structure is a 3' end.
147. The kit of claim 145 wherein each target sequence comprises a
5' region and a 3' region, wherein the open circle probes each
comprise a single-stranded, linear DNA molecule comprising, from 5'
end to 3' end, a 5' phosphate group, a right target probe portion,
a spacer portion, a left target probe portion, and a 3' hydroxyl
group, wherein the spacer portion comprises a primer complement
portion, wherein the left target probe portion is complementary to
the 3' region of at least one of the target sequences and the right
target probe portion is complementary to the 5' region of the same
target sequence, wherein the rolling circle replication primer
comprises a single-stranded, linear nucleic acid molecule
comprising a complementary portion that is complementary to the
primer complement portion of one or more of the open circle
probes.
148. The kit of claim 47 further comprising a secondary DNA strand
displacement primer comprising a single-stranded, linear nucleic
acid molecule comprising a matching portion that matches a portion
of one or more of the open circle probes.
149. The kit of claim 147 wherein at least one target sequence
further comprises a central region located between the 5' region
and the 3' region, wherein neither the left target probe portion
nor the right target probe portion of the open circle probe
complementary to the target sequence is complementary to the
central region of the target sequence.
150. The kit of claim 149 further comprising one or more gap
oligonucleotides, wherein the gap oligonucleotides are
complementary to all or a portion of the central region of the
target sequence.
151. The kit of claim 147 the target probe portions of the open
circle probes are complementary to a different target sequence for
each of a plurality of the open circle probes.
152. The kit of claim 151 further comprising one or more detection
probes.
153. The kit of claim 152 wherein a portion of each of a plurality
of the detection probes has sequence matching or complementary to a
portion of a different one of the open circle probes.
154. The kit of claim 145 further comprising one or more reporter
binding agents each comprising a specific binding molecule and an
oligonucleotide portion, wherein the oligonucleotide portion
comprises one of the target sequences.
155. The kit of claim 154 wherein the specific binding molecule of
at least one of the reporter binding agents is specific for a
protein or peptide.
156. The kit of claim 155 wherein the specific binding molecules of
a plurality of the reporter binding agents are each specific for a
different protein or peptide.
157. The kit of claim 154 wherein the specific binding molecule of
at least one of the reporter binding agents is specific for an
analyte.
158. The kit of claim 157 wherein the specific binding molecules of
a plurality of the reporter binding agents are each specific for a
different analyte.
159. The kit of claim 145 wherein the portions of the open circle
probes that are complementary to the target sequence are
complementary to a different target sequence for each of a
plurality of the open circle probes.
160. The kit of claim 147 wherein a plurality of the open circle
probes are specific for different target sequences.
161. A kit for selectively detecting one or more target sequences
or selectively amplifying nucleic acid sequences related to one or
more target sequences, the kit comprising, a plurality of open
circle probes each comprising two ends, wherein at least one of the
ends of one of the open circle probe can form an intramolecular
stem structure, wherein portions of each open circle probe are
complementary to the one or more target sequences, wherein the
portions of the open circle probes that are complementary to the
target sequence are complementary to a different target sequence
for each of a plurality of the open circle probes, one or more
rolling circle replication primers, wherein all or a portion of
each rolling circle replication primer is complementary to a
portion of one or more of the open circle probes, and a plurality
of detection probes, wherein a portion of each of a plurality of
the detection probes has sequence matching or complementary to a
portion of a different one of the open circle probes.
162. A kit for selectively detecting one or more target sequences
or selectively amplifying nucleic acid sequences related to one or
more target sequences, the kit comprising, a plurality of open
circle probes each comprising two ends, wherein at least one of the
ends of one of the open circle probe can form an intramolecular
stem structure, wherein portions of each open circle probe are
complementary to the one or more target sequences, wherein the
portions of the open circle probes that are complementary to the
target sequences are complementary to a different target sequence
for each of a plurality of the open circle probes, one or more
rolling circle replication primers, wherein all or a portion of
each rolling circle replication primer is complementary to a
portion of one or more of the open circle probes, and a plurality
of reporter binding agents each comprising a specific binding
molecule and an oligonucleotide portion, wherein the
oligonucleotide portion comprises one of the target sequences.
163. The kit of claim 162 further comprising a plurality of
detection probes, wherein a portion of each of a plurality of the
detection probes has sequence matching or complementary to a
portion of a different one of the open circle probes.
Description
[0001] The present invention is in the field of nucleic acid
amplification, and specifically in the area of reducing
non-specific amplification in nucleic acid amplification
reactions.
BACKGROUND OF THE INVENTION
[0002] Numerous nucleic acid amplification techniques have been
devised, including strand displacement cascade amplification
(SDCA)(referred to herein as exponential rolling circle
amplification (ERCA)) and rolling circle amplification (RCA)(U.S.
Pat. No. 5,854,033; PCT Application No. WO 97/19193; Lizardi et
al., Nature Genetics 19(3):225-232 (1998)); multiple displacement
amplification (MDA)(PCT Application WO 99/18241); strand
displacement amplification (SDA)(Walker et al., Nucleic Acids
Research 20:1691-1696 (1992), Walker et al., Proc. Natl. Acad. Sci.
USA 89:392-396 (1992)); polymerase chain reaction (PCR) and other
exponential amplification techniques involving thermal cycling,
self-sustained sequence replication (3SR), nucleic acid sequence
based amplification (NASBA), and amplification with Q.beta.
replicase (Birkenmeyer and Mushahwar, J. Virological Methods
35:117-126 (1991); Landegren, Trends Genetics 9:199-202 (1993));
and various linear amplification techniques involving thermal
cycling such as cycle sequencing (Craxton et al., Methods Companion
Methods in Enzymology 3:20-26 (1991)).
[0003] Rolling Circle Amplification (RCA) driven by DNA polymerase
can replicate circular oligonucleotide probes with either linear or
geometric kinetics under isothermal conditions (Lizardi et al.,
Nature Genet. 19: 225-232 (1998); U.S. Pat. Nos. 5, 854,033 and
6,143,495; PCT Application No. WO 97/19193). If a single primer is
used, RCA generates in a few minutes a linear chain of hundreds or
thousands of tandemly-linked DNA copies of a target that is
covalently linked to that target. Generation of a linear
amplification product permits both spatial resolution and accurate
quantitation of a target. DNA generated by RCA can be labeled with
fluorescent oligonucleotide tags that hybridize at multiple sites
in the tandem DNA sequences. RCA can be used with fluorophore
combinations designed for multiparametric color coding (PCT
Application No. WO 97/19193), thereby markedly increasing the
number of targets that can be analyzed simultaneously. RCA
technologies can be used in solution, in situ and in microarrays.
In solid phase formats, detection and quantitation can be achieved
at the level of single molecules (Lizardi et al., 1998).
Ligation-mediated Rolling Circle Amplification (LM-RCA) involves
circularization of a probe molecule hybridized to a target sequence
and subsequent rolling circle amplification of the circular probe
(U.S. Pat. Nos. 5, 854,033 and 6,143,495; PCT Application No. WO
97/19193).
[0004] Artifacts--that is, unwanted, unexpected, or non-specific
nucleic acid molecules--have been observed in almost all nucleic
acid amplification reactions. For example, Stump et al., Nucleic
Acids Research 27:4642-4648 (1999), describes nucleic acid
artifacts resulting from an illegitimate PCR process during cycle
sequencing. In rolling circle amplification, uncircularized open
circle probes could prime synthesis during amplification of
circularized open circle probes. Other forms of artifacts can occur
in other types of nucleic acid amplification techniques.
[0005] Therefore, it is an object of the present invention to
provide a method of reducing, preventing, or eliminating artifacts
in nucleic acid amplification reactions.
[0006] It is another object of the present invention to provide
open circle probes and primers that, when used in a nucleic acid
amplification reaction, can reduce, prevent, or eliminate artifacts
in the nucleic acid amplification reaction.
[0007] It is another object of the present invention to provide
kits for nucleic acid amplification that can reduce, prevent, or
eliminate artifacts in the nucleic acid amplification reaction.
BRIEF SUMMARY OF THE INVENTION
[0008] Disclosed are compositions and methods for reducing or
eliminating generation of unwanted, undesirable, or non-specific
amplification products in nucleic acid amplification reactions. One
form of composition is an open circle probe that can form an
intramolecular stem structure, such as a hairpin structure, at one
or both ends. Open circle probes are useful in rolling circle
amplification techniques. The stem structure allows the open circle
probe to be circularized when hybridized to a legitimate target
sequence but results in inactivation of uncircularized open circle
probes. This inactivation, which preferably involves stabilization
of the stem structure, extension of the end of the open circle
probe, or both, reduces or eliminates the ability of the open
circle probe to prime nucleic acid synthesis or to serve as a
template for rolling circle amplification.
[0009] In ligation-mediated rolling circle amplification, a linear
DNA molecule, referred to as an open circle probe or padlock probe,
hybridizes to a target sequence and is circularized. The
circularized probe is then amplified via rolling circle replication
of the circular probe. Uncircularized probe that remains in the
reaction can hybridize to nucleic acid sequences in the reaction
and cause amplification of undesirable, non-specific sequences. The
disclosed compositions and method address this problem by reducing
or eliminating the potential uncircularized open circle probes from
priming nucleic acid synthesis. A basic form of the disclosed
method involves use of the disclosed open circle probes in a
rolling circle amplification reaction or assay.
[0010] The disclosed open circle probes can be inactivated in
several ways. For example, where the 3' end of an open circle probe
is involved in an intramolecular stem structure, the 3' end can be
extended in a replication reaction using the open circle probe
sequences as template. Stabilization of the stem structure results
in a reduction or elimination of the ability of the open circle
probe to prime nucleic acid synthesis because the 3' end is stably
hybridized to sequences in the open circle probe under the
conditions used for nucleic acid replication. The open circle probe
can also be inactivated by formation of the intramolecular stem
structure during the amplification reaction. As long as the end
remains in the intramolecular stem structure, it is not available
for priming nucleic acid synthesis. A preferred form of open circle
probe includes a loop as part of the intramolecular stem structure.
Hybridization of the loop to the target sequence disrupts the
intramolecular stem structure while hybridization of the loop to a
mismatched or non-target sequence will not. Thus, the
sequence-discrimination ability of the open circle probe determines
inactivation of the open circle probe. A hybridization nucleating
loop can also be used in linear primers used for nucleic acid
replication and amplification.
[0011] The disclosed method is useful for detection, quantitation,
and/or location of any desired analyte, such as proteins and
peptides. The disclosed method can be multiplexed to detect
numerous different analytes simultaneously or used in a single
assay. Thus, the disclosed method is useful for detecting,
assessing, quantitating, profiling, and/or cataloging gene
expression and the presence of nucleic acids and protein in
biological samples. The disclosed method is also particularly
useful for detecting and discriminating single nucleotide
differences in nucleic acid sequences. Thus, the disclosed method
is useful for extensive multiplexing of target sequences for
sensitive and specific detection of the target sequences themselves
or analytes to which the target sequences have been associated. The
disclosed method is applicable to numerous areas including, but not
limited to, analysis of proteins present in a sample (for example,
proteomics analysis), disease detection, mutation detection,
protein expression profiling, RNA expression profiling, gene
discovery, gene mapping (molecular haplotyping), agricultural
research, and virus detection.
BRIEF DESCRIPTION OF THE DRAWINGS
[0012] FIG. 1 is a diagram illustrating an open circle probe that
forms hairpin intramolecular stem structures at both ends (top
left). The open circle probe is shown hybridized to a target
sequence and ligated (top right). Possible intramolecular
structures formed by the ligated open circle probe are also shown
(bottom).
[0013] FIGS. 2A and 2B are diagrams illustrating an open circle
probe that forms a stem and loop intramolecular stem structure. If
the target sequence is present, the open circle probe will
hybridize to the target sequence, be ligated, and serve as a
template in rolling circle amplification (FIG. 2A). If the target
sequence is not present, the intramolecular structure remains and
the 3' end of the open circle probe is extended using the "other"
strand as template (FIG. 2B).
[0014] FIG. 3 is a diagram illustrating hybridization, ligation,
and amplification of an open circle probe that forms a stem and
loop intramolecular stem structure. Hybridization to the target
sequence is nucleated by interaction between nucleotides in the
loop of the open circle probe and nucleotides in the target
sequence (left). This nucleation causes the intramolecular stem
structure to be disrupted (middle bottom). The freed end can now
hybridize to the target sequence, adjacent to the other end of the
probe (right bottom). The open circle probe can then be ligated,
thus circularizing the probe, followed by rolling circle
amplification of the circularized probe (right top).
[0015] FIGS. 4A, 4B, and 4C are diagrams illustrating hybridization
of an open circle probe that forms a stem and loop intramolecular
stem structure to a non-target sequence. In most cases,
hybridization of loop sequences to a non-target sequence will leave
the intramolecular stem structure intact (FIG. 4B). The open circle
probe will not be circularized. Even if hybridization of the loop
to a non-target sequence were to disrupt the intramolecular stem
structure, the non-target sequence is unlikely to have nucleotides
complementary to end sequences of the open circle probe (FIG.
4C).
[0016] FIG. 5 depicts graphs of delta Ct versus time obtained from
a real time ERCA reaction (Example 2). Delta Ct=(Ct minus ligase
control-Ct plus ligase).
DETAILED DESCRIPTION OF THE INVENTION
[0017] Disclosed are compositions and methods for reducing or
eliminating generation of unwanted, undesirable, or non-specific
amplification products in nucleic acid amplification reactions. One
form of composition is an open circle probe that can form an
intramolecular stem structure, such as a hairpin structure, at one
or both ends. Open circle probes are useful in rolling circle
amplification techniques. The stem structure allows the open circle
probe to be circularized when hybridized to a legitimate target
sequence but results in inactivation of uncircularized open circle
probes. This inactivation, which preferably involves stabilization
of the stem structure, extension of the end of the open circle
probe, or both, reduces or eliminates the ability of the open
circle probe to prime nucleic acid synthesis or to serve as a
template for rolling circle amplification.
[0018] In ligation-mediated rolling circle amplification (LM-RCA),
a linear DNA molecule, referred to as an open circle probe or
padlock probe, hybridizes to a target sequence and is circularized.
The circularized probe is then amplified via rolling circle
replication of the circular probe. Uncircularized probe that
remains in the reaction can hybridize to nucleic acid sequences in
the reaction and cause amplification of undesirable, non-specific
sequences. The disclosed compositions and method address this
problem by reducing or eliminating the potential uncircularized
open circle probes from priming nucleic acid synthesis.
[0019] The disclosed open circle probes can be inactivated in
several ways. For example, where the 3' end of an open circle probe
is involved in an intramolecular stem structure, the 3' end can be
extended in a replication reaction using the open circle probe
sequences as template (see FIG. 2B). The result is stabilization of
the intramolecular stem structure and a change in the 3' end
sequence. Stabilization of the stem structure results in a
reduction or elimination of the ability of the open circle probe to
prime nucleic acid synthesis because the 3' end is stably
hybridized to sequences in the open circle probe under the
conditions used for nucleic acid replication. Change in the
sequence of the 3' end can reduce of the ability of the open circle
probe to prime nucleic acid synthesis because the changed 3'
sequences may not be as closely related to sequences involved in
the amplification reaction or assay. Change in the sequence of the
3' end can reduce of the ability of the open circle probe to serve
as a template for rolling circle amplification. For example, even
if the open circle probe with extended 3' end were circularized,
the rolling circle replication primer could be prevented from
priming replication of such a circle if the primer complement
sequence on the open circle probe were interrupted by the added
sequences. This can be accomplished by, for example, designing the
open circle probe to have the primer complement sequence include
both 5' and 3' end sequences of the open circle probe.
[0020] The open circle probe can also be inactivated by formation
of the intramolecular stem structure during the amplification
reaction. As long as the end remains in the intramolecular stem
structure, it is not available for priming nucleic acid synthesis.
This form of inactivation is aided by design the intramolecular
stem structure, or selecting amplification conditions, such that
the intramolecular hybrid remains stable during rolling circle
amplification.
[0021] One form of the disclosed open circle probes includes a loop
as part of the intramolecular stem structure. It is preferred that
the loop contain sequences complementary to the target sequence.
This allows the loop to nucleate hybridization of the open probe to
the target sequence. Preferred forms of the loop-containing probes
are characterized by a sequence discrimination capability that is
markedly better that the comparable linear probes due to the
competition between the structural interferences between folding
due to intramolecular stem formation and linear rigidity due to
hybridization of the probe sequence to the target (Tyagi and
Kramer, Nat Biotechnol 14(3):303-8 (1996); Bonnet et al., Proc Natl
Acad Sci USA 96(11):6171-6 (1999)). Preferred open circle probes of
this type will not hybridize to mismatched sequences under suitable
conditions because duplex hybridization of probe to target does not
effectively compete with intramolecular stem formation of the
structured probe. This makes the end(s) of the open circle probe
involved in an intramolecular stem structure unavailable for
ligation to circularize the probe and leave the 3' end available
for inactivating extension. The presence of target sequence causes
the correctly matched open circle probe to unfold, allowing the
ends to hybridize to the target sequence and be coupled (see FIG.
3). Where sequences in the loop nucleate hybridization of the open
circle probe to a target sequence, loop hybridization to a
non-target sequence is unlikely to lead to circularization of the
open circle probe. This is because it is unlikely that a non-target
sequence will include adjacent sequences to which both the loop and
open circle probe end can hybridize (see FIG. 4).
[0022] A hybridization nucleating loop can also be used in linear
primers used for nucleic acid replication and amplification. Such a
primer forms an intramolecular stem structure, including a loop.
Loop-containing primers of this type will not hybridize to
mismatched sequences under suitable conditions because duplex
hybridization of probe to target does not effectively compete with
intramolecular stem formation of the structured probe. This makes
the end of the primer involved in an intramolecular stem structure
unavailable for priming. The legitimate primer complement sequence
causes the correctly matched primer to unfold, allowing the end to
hybridize to the primer complement sequence and prime synthesis.
Where sequences in the loop nucleate hybridization of the primer,
loop hybridization to an illegitimate sequence is unlikely to lead
to priming. This is because it is unlikely that an illegitimate
sequence will include adjacent sequences to which both the loop and
the primer end can hybridize. Including proximity-sensitive labels
used in molecular beacon probes in such primers allows
hybridization and priming by the primers to be detected through
activation of the label upon disruption of the intramolecular stem
structure (Tyagi and Kramer, Nat Biotechnol 14(3):303-8 (1996);
Bonnet et al., Proc Natl Acad Sci USA 96(11):6171-6 (1999)).
[0023] The disclosed method is useful for detection, quantitation,
and/or location of any desired analyte. The disclosed method can be
multiplexed to detect numerous different analytes simultaneously or
used in a single assay. Thus, the disclosed method is useful for
detecting, assessing, quantitating, profiling, and/or cataloging
gene expression and the presence of protein in biological samples.
The disclosed method is also particularly useful for detecting and
discriminating single nucleotide differences in nucleic acid
sequences. This specificity is possible due to the sensitivity of
the intramolecular stem structure in loop-containing probes and
primers to mismatches between the loop sequence and a prospective
target sequence. Thus, the disclosed method is useful for extensive
multiplexing of target sequences for sensitive and specific
detection of the target sequences themselves or analytes to which
the target sequences have been associated. The disclosed method is
also useful for detecting, assessing, quantitating, and/or
cataloging single nucleotide polymorphisms, and other sequence
differences between nucleic acids, nucleic acid samples, and
sources of nucleic acid samples.
[0024] The disclosed method is useful for detecting any desired
sequence or other analyte, such as proteins and peptides. In
particular, the disclosed method can be used to localize or amplify
signal from any desired analyte. For example, the disclosed method
can be used to assay tissue, transgenic cells, bacterial or yeast
colonies, cellular material (for example, whole cells, proteins,
DNA fibers, interphase nuclei, or metaphase chromosomes on slides,
arrayed genomic DNA, RNA), and samples and extracts from any of
biological source. Where target sequences are associated with an
analyte, different target sequences, and thus different analytes,
can be sensitively distinguished. Specificity of such detection is
aided by sensitivity of a loop in an open circle probe to
mismatches.
[0025] The disclosed method is applicable to numerous areas
including, but not limited to, analysis of proteins present in a
sample (for example, proteomics analysis), disease detection,
mutation detection, protein expression profiling, RNA expression
profiling, gene discovery, gene mapping (molecular haplotyping),
agricultural research, and virus detection. Preferred uses include
protein and peptide detection in situ in cells, on microarrays,
protein expression profiling; mutation detection; detection of
abnormal proteins or peptides (for example, overexpression of an
oncogene protein or absence of expression of a tumor suppressor
protein); expression in cancer cells; detection of viral proteins
in cells; viral protein expression; detection of inherited diseases
such as cystic fibrosis, muscular dystrophy, diabetes, hemophilia,
sickle cell anemia; assessment of predisposition for cancers such
as prostate cancer, breast cancer, lung cancer, colon cancer,
ovarian cancer, testicular cancer, pancreatic cancer. The disclosed
method can also be used for detection of nucleic acids in situ in
cells, on microarrays, on DNA fibers, and on genomic DNA arrays;
detection of RNA in cells; RNA expression profiling; molecular
haplotyping; mutation detection; detection of abnormal RNA (for
example, overexpression of an oncogene or absence of expression of
a tumor suppressor gene); expression in cancer cells; detection of
viral genome in cells; viral RNA expression; detection of inherited
diseases such as cystic fibrosis, muscular dystrophy, diabetes,
hemophilia, sickle cell anemia; assessment of predisposition for
cancers such as prostate cancer, breast cancer, lung cancer, colon
cancer, ovarian cancer, testicular cancer, pancreatic cancer.
[0026] Rolling Circle Amplification
[0027] The disclosed probes and primers are generally useful in
rolling circle amplification (RCA) reactions. Rolling circle
amplification is described in U.S. Pat. Nos. 5,854,033 and
6,143,495. Rolling circle amplification involves amplifying nucleic
acid sequences based on the presence of a specific target sequence
or analyte, such as a protein or peptide. The method is useful for
detecting specific nucleic acids or analytes in a sample with high
specificity and sensitivity. The method also has an inherently low
level of background signal. Preferred embodiments of the method,
referred to as ligation-mediated RCA (LM-RCA), consist of a DNA
ligation operation, an amplification operation, and, optionally, a
detection operation. The DNA ligation operation circularizes a
specially designed nucleic acid probe molecule (referred to as an
open circle probe). This step is dependent on hybridization of the
probe to a target sequence and forms circular probe molecules in
proportion to the amount of target sequence present in a sample.
The amplification operation is rolling circle replication of the
circularized probe. By coupling a nucleic acid tag to a specific
binding molecule, such as an antibody, amplification of the nucleic
acid tag can be used to detect analytes in a sample. This is
preferred for detection of analytes where a target nucleic acid
sequence is part of a reporter binding molecule, where an
amplification target circle serves as an amplifiable tag on a
reporter binding molecule, or where an amplification target circle
is amplified using a rolling circle replication primer that is part
of a reporter binding molecule. Optionally, an additional
amplification operation can be performed on the DNA produced by
rolling circle replication. Rolling circle amplification can also
be performed independently of a ligation operation.
[0028] Following amplification, the amplified sequences can be
detected and quantified using any of the conventional detection
systems for nucleic acids such as detection of fluorescent labels,
enzyme-linked detection systems, antibody-mediated label detection,
and detection of radioactive labels. Major advantages of this
method are that the ligation operation can be manipulated to obtain
allelic discrimination and the amplification operation is
isothermal. In multiplex assays, the primer oligonucleotide used
for DNA replication can be the same for all probes, or subsets of
probes can be used for different sets of amplified nucleic acids to
be detected. Rolling circle amplification is especially suited to
sensitive detection of multiple analytes, such as proteins and
peptides, in a single assay, reaction, or assay system.
[0029] Rolling circle amplification has two features that provide
simple and consistent amplification and detection of a target
nucleic acid sequence. First, target sequences are amplified via a
small diagnostic probe with an arbitrary primer binding sequence.
This allows consistency in the priming and replication reactions,
even between probes having very different target sequences. Second,
amplification takes place not in cycles, but in a continuous,
isothermal replication: rolling circle replication. This makes
amplification less complicated and much more consistent in
output.
[0030] The disclosed compositions can also be used in methods for
of multiplex detection of molecules of interest involving rolling
circle replication. The methods are useful for simultaneously
detecting multiple specific nucleic acids in a sample with high
specificity and sensitivity. The methods also have an inherently
low level of background signal. A preferred form of such a method
consists of an association operation, an amplification operation,
and a detection operation. The method preferably also includes a
ligation operation. The association operation involves association
of one or more specially designed reporter binding molecules,
either wholly or partly nucleic acid, to target molecules of
interest. The reporter binding molecules can target any molecule of
interest but preferably target proteins or peptides. This operation
associates the reporter binding molecules to a target molecules
present in a sample. The amplification operation is rolling circle
replication of circular nucleic acid molecules, termed
amplification target circles, that are either a part of, or
hybridized to, the probe molecules. By coupling a nucleic acid tag
to a specific binding molecule, such as an antibody, amplification
of the nucleic acid tag can be used to detect analytes in a
sample.
[0031] Following rolling circle replication, the amplified
sequences can be detected using combinatorial multicolor coding
probes (or other multiplex detection system) that allow separate
and simultaneous detection of multiple different amplified target
sequences representing multiple different target molecules. Major
advantages of this method are that a large number of distinct
target molecules can be detected simultaneously, and that
differences in the amounts of the various target molecules in a
sample can be accurately quantified. The target molecules can be
analytes of any nature (such as proteins and peptides) by
associating the target sequences to be amplified with the target
molecules.
[0032] Materials
[0033] A. Open Circle Probes
[0034] An open circle probe (OCP) is a linear single-stranded DNA
molecule, preferably containing between 50 to 1000 nucleotides,
more preferably between about 60 to 150 nucleotides, and most
preferably between about 70 to 100 nucleotides. The OCP has a 5'
phosphate group and a 3' hydroxyl group. This allows the ends to be
ligated (to each other or to other nucleic acid ends) using a
ligase, coupled, or extended in a gap-filling operation. Preferred
open circle probes for use in the disclosed method can form an
intramolecular stem structure involving one or both of the OCP's
ends. Such open circle probes are referred to herein as hairpin
open circle probes. An intramolecular stem structure involving an
end refers to a stem structure where the terminal nucleotides (that
is, nucleotides at the end) of the OCP are hybridized to other
nucleotides in the OCP (FIGS. 1 and 2).
[0035] The intramolecular stem structure can form a hairpin
structure or a stem and loop structure. If both ends of an OCP are
involved in an intramolecular stem structure, the two ends of the
OCP can each form a separate intramolecular stem structure or can
together form a single intramolecular stem structure. In the latter
case the two ends would be hybridized together. It is preferred
that the 3' end of the open circle probe form an intramolecular
stem structure. The 5' end of the open circle probe can also form
an intramolecular stem structure, either alone, or in the same open
circle probe having an intramolecular stem structure at the 3' end.
The intramolecular stem structure preferably forms under conditions
suitable for nucleic acid replication, and in particular under
conditions used for nucleic acid replication when the open circle
probe is being used. For example, the intramolecular stem structure
can be designed to form under conditions used for rolling circle
replication. The formation of the intramolecular stem structure
during replication allows the structure to reduce or prevent
participation of uncircularized open circle probes in nucleic acid
replication. In particular, the intramolecular stem structure
prevents the open circle probe in which the structure forms from
serving as a template for rolling circle replication, from priming
nucleic acid replication, or both. This follows from the
sequestration of the end of uncircularized open circle probe in the
stem. The end of the open circle probe cannot hybridize to, and
prime from, another sequence while sequestered in the
intramolecular stem structure. It is also preferred that the
intramolecular stem structure be more stable than hybrids between
the open circle probe and mismatched sequences. In this way, the
intramolecular stem structure will be thermodynamically favored
over undesired primer hybridizations. Open circle probes that form
intramolecular stem structures at the 3' end will have the 3' end
extended during replication (using open circle probe sequences as
template). This serves to stabilize the intramolecular stem
structure in the uncircularized open circle probes, making them
unavailable for priming.
[0036] Portions of the OCP have specific functions making the OCP
useful for RCA and LM-RCA. These portions are referred to as the
target probe portions, the primer complement portion, the spacer
region, the detection tag portions, the secondary target sequence
portions, the address tag portions, and the promoter portion. The
target probe portions and the primer complement portion are
required elements of an open circle probe. The primer complement
portion is preferably part of the spacer region. Detection tag
portions, secondary target sequence portions, and promoter portions
are optional and, when present, are part of the spacer region.
Address tag portions are optional and, when present, may be part of
the spacer region. The primer complement portion, and the detection
tag portions, the secondary target sequence portions, the address
tag portions, and the promoter portion, if present, are preferably
non-overlapping. However, various of these portions can be
partially or completely overlapping if desired. Generally, an open
circle probe is a single-stranded, linear DNA molecule comprising,
from 5' end to 3' end, a 5' phosphate group, a right target probe
portion, a spacer region, a left target probe portion, and a 3'
hydroxyl group, with a primer complement portion present as part of
the spacer region. Those segments of the spacer region that do not
correspond to a specific portion of the OCP can be arbitrarily
chosen sequences. It is preferred that OCPs do not have any
sequences that are self-complementary. It is considered that this
condition is met if there are no complementary regions greater than
six nucleotides long without a mismatch or gap. It is also
preferred that OCPs containing a promoter portion do not have any
sequences that resemble a transcription terminator, such as a run
of eight or more thymidine nucleotides.
[0037] The open circle probe, when ligated and replicated, gives
rise to a long DNA molecule containing multiple repeats of
sequences complementary to the open circle probe. This long DNA
molecule is referred to herein as tandem sequences DNA (TS-DNA).
TS-DNA contains sequences complementary to the target probe
portions, the primer complement portion, the spacer region, and, if
present on the open circle probe, the detection tag portions, the
secondary target sequence portions, the address tag portions, and
the promoter portion. These sequences in the TS-DNA are referred to
as target sequences (which match the original target sequence),
primer sequences (which match the sequence of the rolling circle
replication primer), spacer sequences (complementary to the spacer
region), detection tags, secondary target sequences, address tags,
and promoter sequences.
[0038] 1. Target Probe Portions
[0039] There are two target probe portions on each OCP, one at each
end of the OCP. The target probe portions can each be any length
that supports specific and stable hybridization between the target
probes and the target sequence. For this purpose, a length of 10 to
35 nucleotides for each target probe portion is preferred, with
target probe portions 15 to 25 nucleotides long being most
preferred. The target probe portion at the 3' end of the OCP is
referred to as the left target probe, and the target probe portion
at the 5' end of the OCP is referred to as the right target probe.
These target probe portions are also referred to herein as left and
right target probes or left and right probes. The target probe
portions are complementary to a target nucleic acid sequence.
[0040] The target probe portions are complementary to the target
sequence, such that upon hybridization the 5' end of the right
target probe portion and the 3' end of the left target probe
portion are base-paired to adjacent nucleotides in the target
sequence, with the objective that they serve as a substrate for
ligation.
[0041] Where the intramolecular stem structure of an open circle
probe forms a stem and loop structure, it is preferred that a
portion of one of the target probe portions of the open circle
probe is in the loop of the stem and loop structure. This portion
of the target probe portion in the loop can then hybridize to the
target sequence of the open circle probe. Such an arrangement
allows design of hairpin open circle probes where the stability of
the intramolecular stem structure depends on the presence or
absence of the specific target sequence. In particular, an open
circle probe that forms a stem and loop structure with a portion of
the target probe portion in the loop can be designed so that
hybridization of the target probe portion in the loop to the target
sequence disrupts the intramolecular stem structure (FIG. 2; Tyagi
and Kramer, Nat Biotechnol 14(3):303-8 (1996); Bonnet et al., Proc
Natl Acad Sci USA 96(11):6171-6 (1999)). In this way, the
intramolecular stem structure remains intact in the absence of the
target sequence and thus reduces or eliminates the ability of the
open circle probe to prime nucleic acid replication (or to serve as
a template for rolling circle replication). In the presence of the
target sequence, disruption of the intramolecular stem structure
allows the end of the open circle probe to hybridize to the target
sequence. This hybrid between the target sequence and the end of
the open circle probe allows the ends of the open circle probe to
come into proximity on the target sequence which in turn allows
ligation of the ends (FIG. 3). For this form of hairpin open circle
probe, it is preferred that hybridization of the loop to a sequence
other than the target sequence does not disrupt the intramolecular
stem structure. Preferably, the hybrid between the target sequence
and the target probe portion at the end of the open circle probe is
more stable than the intramolecular stem structure. This helps
stabilize hybridization of the open circle probe to the target
sequence in competition with the intramolecular stem structure.
[0042] Discrimination of open circle probe hybridization also can
be accomplished by hybridizing probe to target sequence under
conditions that favor only exact sequence matches leaving other
open circle probes unhybridized. The unhybridized open circle
probes will retain or re-form the intramolecular hybrid and the end
of the open circle probe involved in the intramolecular stem
structure will be extended during replication.
[0043] In another form of open circle probe, the 5' end and the 3'
end of the target probe portions may hybridize in such a way that
they are separated by a gap space. In this case the 5' end and the
3' end of the OCP may only be ligated if one or more additional
oligonucleotides, referred to as gap oligonucleotides, are used, or
if the gap space is filled during the ligation operation. The gap
oligonucleotides hybridize to the target sequence in the gap space
to a form continuous probe/target hybrid. The gap space may be any
length desired but is generally ten nucleotides or less. It is
preferred that the gap space is between about three to ten
nucleotides in length, with a gap space of four to eight
nucleotides in length being most preferred. Alternatively, a gap
space could be filled using a DNA polymerase during the ligation
operation. When using such a gap-filling operation, a gap space of
three to five nucleotides in length is most preferred. As another
alternative, the gap space can be partially bridged by one or more
gap oligonucleotides, with the remainder of the gap filled using
DNA polymerase.
[0044] 2. Primer Complement Portion
[0045] The primer complement portion is part of the spacer region
of an open circle probe. The primer complement portion is
complementary to the rolling circle replication primer (RCRP). Each
OCP preferably has a single primer complement portion. This allows
rolling circle replication to initiate at a single site on ligated
OCPs. The primer complement portion and the cognate primer can have
any desired sequence so long as they are complementary to each
other. The sequence of the primer complement portion is referred to
as the primer complement sequence. In general, the sequence of the
primer complement can be chosen such that it is not significantly
similar to any other portion of the OCP. The primer complement
portion can be any length that supports specific and stable
hybridization between the primer complement portion and the primer.
For this purpose, a length of 10 to 35 nucleotides is preferred,
with a primer complement portion 16 to 20 nucleotides long being
most preferred. The primer complement portion can be located
anywhere within the spacer region of an OCP. It is preferred that
the primer complement portion is adjacent to the right target
probe, with the right target probe portion and the primer
complement portion preferably separated by three to ten
nucleotides, and most preferably separated by six nucleotides. This
location prevents the generation of any other spacer sequences,
such as detection tags and secondary target sequences, from
unligated open circle probes during DNA replication.
[0046] 3. Detection Tag Portions
[0047] Detection tag portions are part of the spacer region of an
open circle probe. Detection tag portions have sequences matching
the sequence of the complementary portion of detection probes.
These detection tag portions, when amplified during rolling circle
replication, result in TS-DNA having detection tag sequences that
are complementary to the complementary portion of detection probes.
If present, there may be one, two, three, or more than three
detection tag portions on an OCP. It is preferred that an OCP have
two, three or four detection tag portions. Most preferably, an OCP
will have three detection tag portions. Generally, it is preferred
that an OCP have 60 detection tag portions or less. There is no
fundamental limit to the number of detection tag portions that can
be present on an OCP except the size of the QCP. When there are
multiple detection tag portions, they may have the same sequence or
they may have different sequences, with each different sequence
complementary to a different detection probe. It is preferred that
an OCP contain detection tag portions that have the same sequence
such that they are all complementary to a single detection probe.
For some multiplex detection methods, it is preferable that OCPs
contain up to six detection tag portions and that the detection tag
portions have different sequences such that each of the detection
tag portions is complementary to a different detection probe. The
detection tag portions can each be any length that supports
specific and stable hybridization between the detection tags and
the detection probe. For this purpose, a length of 10 to 35
nucleotides is preferred, with a detection tag portion 15 to 20
nucleotides long being most preferred.
[0048] 4. Secondary Target Sequence Portions
[0049] Secondary target sequence portions are part of the spacer
region of an open circle probe. Secondary target sequence portions
have sequences matching the sequence of target probes of a
secondary open circle probe. These secondary target sequence
portions, when amplified during rolling circle replication, result
in TS-DNA having secondary target sequences that are complementary
to target probes of a secondary open circle probe. If present,
there may be one, two, or more than two secondary target sequence
portions on an OCP. It is preferred that an OCP have one or two
secondary target sequence portions. Most preferably, an OCP will
have one secondary target sequence portion. Generally, it is
preferred that an OCP have 50 secondary target sequence portions or
less. There is no fundamental limit to the number of secondary
target sequence portions that can be present on an OCP except the
size of the OCP. When there are multiple secondary target sequence
portions, they may have the same sequence or they may have
different sequences, with each different sequence complementary to
a different secondary OCP. It is preferred that an OCP contain
secondary target sequence portions that have the same sequence such
that they are all complementary to a single target probe portion of
a secondary OCP. The secondary target sequence portions can each be
any length that supports specific and stable hybridization between
the secondary target sequence and the target sequence probes of its
cognate OCP. For this purpose, a length of 20 to 70 nucleotides is
preferred, with a secondary target sequence portion 30 to 40
nucleotides long being most preferred. As used herein, a secondary
open circle probe is an open circle probe where the target probe
portions match or are complementary to secondary target sequences
in another open circle probe or an amplification target circle. It
is contemplated that a secondary open circle probe can itself
contain secondary target sequences that match or are complementary
to the target probe portions of another secondary open circle
probe. Secondary open circle probes related to each other in this
manner are referred to herein as nested open circle probes.
[0050] 5. Address Tag Portion
[0051] The address tag portion is part of either the target probe
portions or the spacer region of an open circle probe. The address
tag portion has a sequence matching the sequence of the
complementary portion of an address probe. This address tag
portion, when amplified during rolling circle replication, results
in TS-DNA having address tag sequences that are complementary to
the complementary portion of address probes. If present, there may
be one, or more than one, address tag portions on an OCP. It is
preferred that an OCP have one or two address tag portions. Most
preferably, an OCP will have one address tag portion. Generally, it
is preferred that an OCP have 50 address tag portions or less.
There is no fundamental limit to the number of address tag portions
that can be present on an OCP except the size of the OCP. When
there are multiple address tag portions, they may have the same
sequence or they may have different sequences, with each different
sequence complementary to a different address probe. It is
preferred that an OCP contain address tag portions that have the
same sequence such that they are all complementary to a single
address probe. Preferably, the address tag portion overlaps all or
a portion of the target probe portions, and all of any intervening
gap space. Most preferably, the address tag portion overlaps all or
a portion of both the left and right target probe portions. The
address tag portion can be any length that supports specific and
stable hybridization between the address tag and the address probe.
For this purpose, a length between 10 and 35 nucleotides long is
preferred, with an address tag portion 15 to 20 nucleotides long
being most preferred.
[0052] 6. Promoter Portion
[0053] The promoter portion corresponds to the sequence of an RNA
polymerase promoter. A promoter portion can be included in an open
circle probe so that transcripts can be generated from TS-DNA. The
sequence of any promoter may be used, but simple promoters for RNA
polymerases without complex requirements are preferred. It is also
preferred that the promoter is not recognized by any RNA polymerase
that may be present in the sample containing the target nucleic
acid sequence. Preferably, the promoter portion corresponds to the
sequence of a T7 or SP6 RNA polymerase promoter. The T7 and SP6 RNA
polymerases are highly specific for particular promoter sequences.
Other promoter sequences specific for RNA polymerases with this
characteristic would also be preferred. Because promoter sequences
are generally recognized by specific RNA polymerases, the cognate
polymerase for the promoter portion of the OCP should be used for
transcriptional amplification. Numerous promoter sequences are
known and any promoter specific for a suitable RNA polymerase can
be used. The promoter portion can be located anywhere within the
spacer region of an OCP and can be in either orientation.
Preferably, the promoter portion is immediately adjacent to the
left target probe and is oriented to promote transcription toward
the 3' end of the open circle probe. This orientation results in
transcripts that are complementary to TS-DNA, allowing independent
detection of TS-DNA and the transcripts, and prevents transcription
from interfering with rolling circle replication.
[0054] B. Gap Oligonucleotides
[0055] Gap oligonucleotides are oligonucleotides that are
complementary to all or a part of that portion of a target sequence
which covers a gap space between the ends of a hybridized open
circle probe. Gap oligonucleotides have a phosphate group at their
5' ends and a hydroxyl group at their 3' ends. This facilitates
ligation of gap oligonucleotides to open circle probes, or to other
gap oligonucleotides. The gap space between the ends of a
hybridized open circle probe can be filled with a single gap
oligonucleotide, or it can be filled with multiple gap
oligonucleotides. For example, two 3 nucleotide gap
oligonucleotides can be used to fill a six nucleotide gap space, or
a three nucleotide gap oligonucleotide and a four nucleotide gap
oligonucleotide can be used to fill a seven nucleotide gap space.
Gap oligonucleotides are particularly useful for distinguishing
between closely related target sequences. For example, multiple gap
oligonucleotides can be used to amplify different allelic variants
of a target sequence. By placing the region of the target sequence
in which the variation occurs in the gap space formed by an open
circle probe, a single open circle probe can be used to amplify
each of the individual variants by using an appropriate set of gap
oligonucleotides.
[0056] C. Amplification Target Circles
[0057] An amplification target circle (ATC) is a circular
single-stranded DNA molecule, preferably containing between 40 to
1000 nucleotides, more preferably between about 50 to 150
nucleotides, and most preferably between about 50 to 100
nucleotides. Portions of ATCs have specific functions making the
ATC useful for rolling circle amplification (RCA). These portions
are referred to as the primer complement portion, the detection tag
portions, the secondary target sequence portions, the address tag
portions, and the promoter portion. The primer complement portion
is a required element of an amplification target circle. Detection
tag portions, secondary target sequence portions, address tag
portions, and promoter portions are optional. The primer complement
portion, and the detection tag portions, the secondary target
sequence portions, the address tag portions, and the promoter
portion, if present, are preferably non-overlapping. However,
various of these portions can be partially or completely
overlapping if desired. Generally, an amplification target circle
is a single-stranded, circular DNA molecule comprising a primer
complement portion. Those segments of the ATC that do not
correspond to a specific portion of the ATC can be arbitrarily
chosen sequences. It is preferred that ATCs do not have any
sequences that are self-complementary. It is considered that this
condition is met if there are no complementary regions greater than
six nucleotides long without a mismatch or gap. It is also
preferred that ATCs containing a promoter portion do not have any
sequences that resemble a transcription terminator, such as a run
of eight or more thymidine nucleotides. Ligated open circle probes
are a type of ATC, and as used herein the term amplification target
circle includes ligated open circle probes. An ATC can be used in
the same manner as described herein for OCPs that have been
ligated.
[0058] An amplification target circle, when replicated, gives rise
to a long DNA molecule containing multiple repeats of sequences
complementary to the amplification target circle. This long DNA
molecule is referred to herein as tandem sequences DNA (TS-DNA).
TS-DNA contains sequences complementary to the primer complement
portion and, if present on the amplification target circle, the
detection tag portions, the secondary target sequence portions, the
address tag portions, and the promoter portion. These sequences in
the TS-DNA are referred to as primer sequences (which match the
sequence of the rolling circle replication primer), spacer
sequences (complementary to the spacer region), detection tags,
secondary target sequences, address tags, and promoter sequences.
Amplification target circles are useful as tags for specific
binding molecules.
[0059] D. Rolling Circle Replication Primer
[0060] A rolling circle replication primer (RCRP) is an
oligonucleotide having sequence complementary to the primer
complement portion of an OCP or ATC. This sequence is referred to
as the complementary portion of the RCRP. The complementary portion
of a RCRP and the cognate primer complement portion can have any
desired sequence so long as they are complementary to each other.
In general, the sequence of the RCRP can be chosen such that it is
not significantly complementary to any other portion of the OCP or
ATC. The complementary portion of a rolling circle replication
primer can be any length that supports specific and stable
hybridization between the primer and the primer complement portion.
Generally this is 10 to 35 nucleotides long, but is preferably 16
to 20 nucleotides long.
[0061] Preferred rolling circle replication primers for use in the
disclosed method can form an intramolecular stem structure
involving one or both of the RCRP's ends. Such rolling circle
replication primers are referred to herein as hairpin rolling
circle replication primers. An intramolecular stem structure
involving an end refers to a stem structure where the terminal
nucleotides (that is, nucleotides at the end) of the RCRP are
hybridized to other nucleotides in the RCRP.
[0062] The intramolecular stem structure can form a hairpin
structure or a stem and loop structure. If both ends of an RCRP are
involved in an intramolecular stem structure, the two ends of the
RCRP can each form a separate intramolecular stem structure or can
together form a single intramolecular stem structure. In the latter
case the two ends would be hybridized together. It is preferred
that the 3' end of the rolling circle replication primer form an
intramolecular stem structure. The 5' end of the rolling circle
replication primer can also form an intramolecular stem structure,
either alone, or in the rolling circle replication primer having an
intramolecular stem structure at the 3' end. The intramolecular
stem structure preferably involves both ends of the primer and has
a blunt end. Also preferred is a short 3' unpaired overhang. The
intramolecular stem structure preferably forms under conditions
suitable for nucleic acid replication, and in particular under
conditions used for nucleic acid replication when the rolling
circle replication primer is being used. For example, the
intramolecular stem structure can be designed to form under
conditions used for rolling circle replication. The formation of
the intramolecular stem structure during replication allows the
structure to reduce or prevent priming by rolling circle
replication primers at unintended sequences. In particular, the
intramolecular stem structure prevents the rolling circle
replication primer in which the structure forms from priming
rolling circle replication, from priming nucleic acid replication,
or both, at sites other than primer complement sequences (that is,
the specific sequences complementary to the complementary portion
of the rolling circle replication primer). This follows from the
sequestration of the end of rolling circle replication primer in
the stem. The end of the rolling circle replication primer cannot
hybridize to, and prime from, another sequence while sequestered in
the intramolecular stem structure. For this purpose, it is
preferred that the intramolecular stem structure be less stable
that the hybrid between the primer complement sequence and the
complementary portion of the rolling circle replication primer (or,
put another way, the hybrid between the primer complement sequence
and the complementary portion of the rolling circle replication
primer should be more stable than the intramolecular stem
structure). It is also preferred that the intramolecular stem
structure be more stable than hybrids between the rolling circle
replication primer and mismatched sequences. In this way, the
intramolecular stem structure will be thermodynamically favored
over undesired primer hybridizations. Although rolling circle
replication primers that form intramolecular stem structures at the
3' end leaving the 5' end unpaired and overhanging can be used,
this is not preferred. In such a case, the 3' end could be extended
during replication (using rolling circle replication primer
sequences as template), thus inactivating the primers.
[0063] Where the intramolecular stem structure of a rolling circle
replication primer forms a stem and loop structure, it is preferred
that a portion of the complementary portion of the rolling circle
replication primer be in the loop of the stem and loop structure.
This portion of the complementary portion in the loop can then
hybridize to the primer complement sequence of the open circle
probe. Such an arrangement allows design of hairpin rolling circle
replication primers where the stability of the intramolecular stem
structure depends on the presence or absence of the specific primer
complement sequence. In particular, a rolling circle replication
primer that forms a stem and loop structure with a portion of the
complementary portion in the loop can be designed so that
hybridization of the complementary portion in the loop to the
primer complement sequence disrupts the intramolecular stem
structure (Tyagi and Kramer, Nat Biotechnol 14(3):303-8 (1996);
Bonnet et al., Proc Natl Acad Sci USA 96(11):6171-6 (1999)). In
this way, the intramolecular stem structure remains intact in the
absence of the primer complement sequence and thus reduces or
eliminates the ability of the rolling circle replication primer to
prime nucleic acid replication. In the presence of the primer
complement sequence, disruption of the intramolecular stem
structure allows the end of the rolling circle replication primer
to hybridize to the primer complement sequence. This hybrid between
the primer complement sequence and the end of the rolling circle
replication primer allows the priming of nucleic acid replication
by the primer. For this form of hairpin rolling circle replication
primer, it is preferred that hybridization of the loop to a
sequence other than the primer complement sequence does not disrupt
the intramolecular stem structure. Preferably, the hybrid between
the primer complement sequence and the end of the rolling circle
replication primer is more stable than the intramolecular stem
structure. This helps stabilize hybridization of the rolling circle
replication primer to the primer complement sequence in competition
with the intramolecular stem structure.
[0064] Discrimination of rolling circle replication primer
hybridization also can be accomplished by hybridizing primer to
primer complement portions of OCPs or ATCs under conditions that
favor only exact sequence matches leaving other rolling circle
replication primer unhybridized. The unhybridized rolling circle
replication primers will retain or re-form the intramolecular
hybrid.
[0065] It is preferred that rolling circle replication primers also
contain additional sequence at the 5' end of the RCRP that is not
complementary to any part of the OCP or ATC. This sequence is
referred to as the non-complementary portion of the RCRP. The
non-complementary portion of the RCRP, if present, serves to
facilitate strand displacement during DNA replication. The
non-complementary portion of a RCRP may be any length, but is
generally 1 to 100 nucleotides long, and preferably 4 to 8
nucleotides long. The rolling circle replication primer may also
include modified nucleotides to make it resistant to exonuclease
digestion. For example, the primer can have three or four
phosphorothioate linkages between nucleotides at the 5' end of the
primer. Such nuclease resistant primers allow selective degradation
of excess unligated OCP and gap oligonucleotides that might
otherwise interfere with hybridization of detection probes, address
probes, and secondary OCPs to the amplified nucleic acid. A rolling
circle replication primer can be used as the tertiary DNA strand
displacement primer in strand displacement cascade
amplification.
[0066] Rolling circle replication primers may also include modified
nucleotides to make them resistant to exonuclease digestion. For
example, the primer can have three or four phosphorothioate
linkages between nucleotides at the 5' end of the primer. Such
nuclease resistant primers allow selective degradation of excess
unligated OCP and gap oligonucleotides that might otherwise
interfere with hybridization of detection probes, address probes,
and secondary OCPs to the amplified nucleic acid.
[0067] E. DNA Strand Displacement Primers
[0068] Primers used for secondary DNA strand displacement are
referred to herein as DNA strand displacement primers. One form of
DNA strand displacement primer, referred to herein as a secondary
DNA strand displacement primer, is an oligonucleotide having
sequence matching part of the sequence of an OCP or ATC. This
sequence is referred to as the matching portion of the secondary
DNA strand displacement primer. This matching portion of a
secondary DNA strand displacement primer is complementary to
sequences in TS-DNA. The matching portion of a secondary DNA strand
displacement primer may be complementary to any sequence in TS-DNA.
However, it is preferred that it not be complementary TS-DNA
sequence matching either the rolling circle replication primer or a
tertiary DNA strand displacement primer, if one is being used. This
prevents hybridization of the primers to each other. The matching
portion of a secondary DNA strand displacement primer may be
complementary to all or a portion of the target sequence. In this
case, it is preferred that the 3' end nucleotides of the secondary
DNA strand displacement primer are complementary to the gap
sequence in the target sequence. It is most preferred that
nucleotide at the 3' end of the secondary DNA strand displacement
primer falls complementary to the last nucleotide in the gap
sequence of the target sequence, that is, the 5' nucleotide in the
gap sequence of the target sequence. The matching portion of a
secondary DNA strand displacement primer can be any length that
supports specific and stable hybridization between the primer and
its complement. Generally this is 12 to 35 nucleotides long, but is
preferably 18 to 25 nucleotides long.
[0069] Preferred secondary DNA strand displacement primers for use
in the disclosed method can form an intramolecular stem structure
involving one or both of the secondary DNA strand displacement
primer's ends. Such secondary DNA strand displacement primers are
referred to herein as hairpin secondary DNA strand displacement
primers. An intramolecular stem structure involving an end refers
to a stem structure where the terminal nucleotides (that is,
nucleotides at the end) of the secondary DNA strand displacement
primer are hybridized to other nucleotides in the secondary DNA
strand displacement primer.
[0070] The intramolecular stem structure can form a hairpin
structure or a stem and loop structure. If both ends of a secondary
DNA strand displacement primer are involved in an intramolecular
stem structure, the two ends of the secondary DNA strand
displacement primer can each form a separate intramolecular stem
structure or can together form a single intramolecular stem
structure. In the latter case the two ends would be hybridized
together. It is preferred that the 3' end of the secondary DNA
strand displacement primer form an intramolecular stem structure.
The 5' end of the secondary DNA strand displacement primer can also
form an intramolecular stem structure, either alone, or in the
secondary DNA strand displacement primer having an intramolecular
stem structure at the 3' end. The intramolecular stem structure
preferably involves both ends of the primer and has a blunt end.
Also preferred is a short 3' unpaired overhang. The intramolecular
stem structure preferably forms under conditions suitable for
nucleic acid replication, and in particular under conditions used
for nucleic acid replication when the secondary DNA strand
displacement primer is being used.
[0071] For example, the intramolecular stem structure can be
designed to form under conditions used for rolling circle
replication. The formation of the intramolecular stem structure
during replication allows the structure to reduce or prevent
priming by secondary DNA strand displacement primers at unintended
sequences. In particular, the intramolecular stem structure
prevents the secondary DNA strand displacement primer in which the
structure forms from priming nucleic acid replication at sites
other than primer complement sequences (that is, the specific
sequences complementary to the complementary portion of the
secondary DNA strand displacement primer) in TS-DNA. This follows
from the sequestration of the end of secondary DNA strand
displacement primer in the stem. The end of the rolling circle
replication primer cannot hybridize to, and prime from, another
sequence while sequestered in the intramolecular stem structure.
For this purpose, it is preferred that the intramolecular stem
structure be less stable that the hybrid between the primer
complement sequence and the complementary portion of the secondary
DNA strand displacement primer (or, put another way, the hybrid
between the primer complement sequence and the matching portion of
the secondary DNA strand displacement primer should be more stable
than the intramolecular stem structure). It is also preferred that
the intramolecular stem structure be more stable than hybrids
between the secondary DNA strand displacement primer and mismatched
sequences. In this way, the intramolecular stem structure will be
thermodynamically favored over undesired primer hybridizations.
Although secondary DNA strand displacement primers that form
intramolecular stem structures at the 3' end leaving the 5' end
unpaired and overhanging can be used, they are not preferred. In
such a case, the 3' end could be extended during replication (using
secondary DNA strand displacement primer sequences as template),
thus inactivating the primers.
[0072] Where the intramolecular stem structure of a secondary DNA
strand displacement primer forms a stem and loop structure, it is
preferred that a portion of the complementary portion of the
secondary DNA strand displacement primer be in the loop of the stem
and loop structure. This portion of the complementary portion in
the loop can then hybridize to the primer complement sequence in
TS-DNA. Such an arrangement allows design of hairpin secondary DNA
strand displacement primers where the stability of the
intramolecular stem structure depends on the presence or absence of
the specific primer complement sequence. In particular, a secondary
DNA strand displacement primer that forms a stem and loop structure
with a portion of the matching portion in the loop can be designed
so that hybridization of the matching portion in the loop to the
primer complement sequence disrupts the intramolecular stem
structure (Tyagi and Kramer, Nat Biotechnol 14(3):303-8 (1996);
Bonnet et al., Proc Natl Acad Sci USA 96(11):6171-6 (1999)). In
this way, the intramolecular stem structure remains intact in the
absence of the primer complement sequence and thus reduces or
eliminates the ability of the secondary DNA strand displacement
primer to prime nucleic acid replication. In the presence of the
primer complement sequence, disruption of the intramolecular stem
structure allows the end of the secondary DNA strand displacement
primer to hybridize to the primer complement sequence. This hybrid
between the primer complement sequence and the end of the secondary
DNA strand displacement primer allows the priming of nucleic acid
replication by the primer. For this form of hairpin secondary DNA
strand displacement primer, it is preferred that hybridization of
the loop to a sequence other than the primer complement sequence
does not disrupt the intramolecular stem structure. Preferably, the
hybrid between the primer complement sequence and the end of the
secondary DNA strand displacement primer is more stable than the
intramolecular stem structure. This helps stabilize hybridization
of the secondary DNA strand displacement primer to the primer
complement sequence in competition with the intramolecular stem
structure.
[0073] Discrimination of secondary DNA strand displacement primer
hybridization also can be accomplished by hybridizing primer to
primer complement portions in TS-DNA under conditions that favor
only exact sequence matches leaving other secondary DNA strand
displacement primer unhybridized. The unhybridized secondary DNA
strand displacement primers will retain or re-form the
intramolecular hybrid.
[0074] It is preferred that secondary DNA strand displacement
primers also contain additional sequence at their 5' end that does
not match any part of the OCP or ATC. This sequence is referred to
as the non-matching portion of the secondary DNA strand
displacement primer. The non-matching portion of the secondary DNA
strand displacement primer, if present, serves to facilitate strand
displacement during DNA replication. The non-matching portion of a
secondary DNA strand displacement primer may be any length, but is
generally 1 to 100 nucleotides long, and preferably 4 to 8
nucleotides long.
[0075] Another form of DNA strand displacement primer, referred to
herein as a tertiary DNA strand displacement primer, is an
oligonucleotide having sequence complementary to part of the
sequence of an OCP or ATC. This sequence is referred to as the
complementary portion of the tertiary DNA strand displacement
primer. This complementary portion of the tertiary DNA strand
displacement primer matches sequences in TS-DNA. The complementary
portion of a tertiary DNA strand displacement primer may be
complementary to any sequence in the OCP or ATC. However, it is
preferred that it not be complementary OCP or ATC sequence matching
the secondary DNA strand displacement primer. This prevents
hybridization of the primers to each other. Preferably, the
complementary portion of the tertiary DNA strand displacement
primer has sequence complementary to a portion of the spacer
portion of an OCP. The complementary portion of a tertiary DNA
strand displacement primer can be any length that supports specific
and stable hybridization between the primer and its complement.
Generally this is 12 to 35 nucleotides long, but is preferably 18
to 25 nucleotides long.
[0076] Preferred tertiary DNA strand displacement primers for use
in the disclosed method can form an intramolecular stem structure
involving one or both of the tertiary DNA strand displacement
primer's ends. Such tertiary DNA strand displacement primers are
referred to herein as hairpin tertiary DNA strand displacement
primers. An intramolecular stem structure involving an end refers
to a stem structure where the terminal nucleotides (that is,
nucleotides at the end) of the tertiary DNA strand displacement
primer are hybridized to other nucleotides in the tertiary DNA
strand displacement primer.
[0077] The intramolecular stem structure can form a hairpin
structure or a stem and loop structure. If both ends of a tertiary
DNA strand displacement primer are involved in an intramolecular
stem structure, the two ends of the tertiary DNA strand
displacement primer can each form a separate intramolecular stem
structure or can together form a single intramolecular stem
structure. In the latter case the two ends would be hybridized
together. It is preferred that the 3' end of the tertiary DNA
strand displacement primer form an intramolecular stem structure.
The 5' end of the tertiary DNA strand displacement primer can also
form an intramolecular stem structure, either alone, or in the
tertiary DNA strand displacement primer having an intramolecular
stem structure at the 3' end. The intramolecular stem structure
preferably forms under conditions suitable for nucleic acid
replication, and in particular under conditions used for nucleic
acid replication when the tertiary DNA strand displacement primer
is being used. For example, the intramolecular stem structure can
be designed to form under conditions used for rolling circle
replication. The formation of the intramolecular stem structure
during replication allows the structure to reduce or prevent
priming by tertiary DNA strand displacement primers at unintended
sequences. In particular, the intramolecular stem structure
prevents the tertiary DNA strand displacement primer in which the
structure forms from priming nucleic acid replication at sites
other than primer complement sequences (that is, the specific
sequences complementary to the complementary portion of the
tertiary DNA strand displacement primer) in TS-DNA. This follows
from the sequestration of the end of tertiary DNA strand
displacement primer in the stem. The end of the rolling circle
replication primer cannot hybridize to, and prime from, another
sequence while sequestered in the intramolecular stem structure.
For this purpose, it is preferred that the intramolecular stem
structure be less stable that the hybrid between the primer
complement sequence and the complementary portion of the tertiary
DNA strand displacement primer (or, put another way, the hybrid
between the primer complement sequence and the complementary
portion of the tertiary DNA strand displacement primer should be
more stable than the intramolecular stem structure). It is also
preferred that the intramolecular stem structure be more stable
than hybrids between the tertiary DNA strand displacement primer
and mismatched sequences. In this way, the intramolecular stem
structure will be thermodynamically favored over undesired primer
hybridizations. Tertiary DNA strand displacement primers that form
intramolecular stem structures at the 3' end will have the 3' end
extended during replication (using tertiary DNA strand displacement
primer sequences as template). This serves to stabilize the
intramolecular stem structure in the tertiary DNA strand
displacement primers, making them unavailable for priming.
[0078] Where the intramolecular stem structure of a tertiary DNA
strand displacement primer forms a stem and loop structure, it is
preferred that a portion of the complementary portion of the
tertiary DNA strand displacement primer be in the loop of the stem
and loop structure. This portion of the complementary portion in
the loop can then hybridize to the primer complement sequence in
TS-DNA. Such an arrangement allows design of hairpin tertiary DNA
strand displacement primers where the stability of the
intramolecular stem structure depends on the presence or absence of
the specific primer complement sequence. In particular, a tertiary
DNA strand displacement primer that forms a stem and loop structure
with a portion of the complementary portion in the loop can be
designed so that hybridization of the complementary portion in the
loop to the primer complement sequence disrupts the intramolecular
stem structure (Tyagi and Kramer, Nat Biotechnol 14(3):303-8
(1996); Bonnet et al., Proc Natl Acad Sci USA 96(11):6171-6
(1999)). In this way, the intramolecular stem structure remains
intact in the absence of the primer complement sequence and thus
reduces or eliminates the ability of the tertiary DNA strand
displacement primer to prime nucleic acid replication. In the
presence of the primer complement sequence, disruption of the
intramolecular stem structure allows the end of the tertiary DNA
strand displacement primer to hybridize to the primer complement
sequence. This hybrid between the primer complement sequence and
the end of the tertiary DNA strand displacement primer allows the
priming of nucleic acid replication by the primer. For this form of
hairpin tertiary DNA strand displacement primer, it is preferred
that hybridization of the loop to a sequence other than the primer
complement sequence does not disrupt the intramolecular stem
structure. Preferably, the hybrid between the primer complement
sequence and the end of the tertiary DNA strand displacement primer
is more stable than the intramolecular stem structure. This helps
stabilize hybridization of the tertiary DNA strand displacement
primer to the primer complement sequence in competition with the
intramolecular stem structure.
[0079] Discrimination of tertiary DNA strand displacement primer
hybridization also can be accomplished by hybridizing primer to
primer complement portions in TS-DNA under conditions that favor
only exact sequence matches leaving other tertiary DNA strand
displacement primer unhybridized. The unhybridized tertiary DNA
strand displacement primers will retain or re-form the
intramolecular hybrid and the end of the tertiary DNA strand
displacement primer involved in the intramolecular stem structure
will be extended during replication.
[0080] It is preferred that tertiary DNA strand displacement
primers also contain additional sequence at their 5' end that is
not complementary to any part of the OCP or ATC. This sequence is
referred to as the non-complementary portion of the tertiary DNA
strand displacement primer. The non-complementary portion of the
tertiary DNA strand displacement primer, if present, serves to
facilitate strand displacement during DNA replication. The
non-complementary portion of a tertiary DNA strand displacement
primer may be any length, but is generally 1 to 100 nucleotides
long, and preferably 4 to 8 nucleotides long. A rolling circle
replication primer is a preferred form of tertiary DNA strand
displacement primer.
[0081] DNA strand displacement primers may also include modified
nucleotides to make them resistant to exonuclease digestion. For
example, the primer can have three or four phosphorothioate
linkages between nucleotides at the 5' end of the primer. Such
nuclease resistant primers allow selective degradation of excess
unligated OCP and gap oligonucleotides that might otherwise
interfere with hybridization of detection probes, address probes,
and secondary OCPs to the amplified nucleic acid. DNA strand
displacement primers can be used for secondary DNA strand
displacement and strand displacement cascade amplification, both
described below and in U.S. Pat. No. 6,143,495.
[0082] F. Reporter Binding Agents
[0083] A reporter binding agent is a specific binding molecule
coupled or tethered to a nucleic acid such as an oligonucleotide.
The specific binding molecule is referred to as the affinity
portion of the reporter binding agent and the nucleic acid is
referred to as the oligonucleotide portion of the reporter binding
agent. As used herein, a specific binding molecule is a molecule
that interacts specifically with a particular molecule or moiety
(that is, an analyte). The molecule or moiety that interacts
specifically with a specific binding molecule is referred to herein
as a target molecule. The target molecules can be any analyte. It
is to be understood that the term target molecule refers to both
separate molecules and to portions of molecules, such as an epitope
of a protein, that interacts specifically with a specific binding
molecule. Antibodies, either member of a receptor/ligand pair, and
other molecules with specific binding affinities are examples of
specific binding molecules, useful as the affinity portion of a
reporter binding molecule. A reporter binding molecule with an
affinity portion which is an antibody is referred to herein as a
reporter antibody. The oligonucleotide portion can be a nucleic
acid molecule or a combination of nucleic acid molecules. The
oligonucleotide portion is preferably an oligonucleotide or an
amplification target circle.
[0084] By tethering an amplification target circle or coupling a
target sequence to a specific binding molecule, binding of a
specific binding molecule to its specific target can be detected by
amplifying the ATC or target sequence with rolling circle
amplification. This amplification allows sensitive detection of a
very small number of bound specific binding molecules. A reporter
binding molecule that interacts specifically with a particular
target molecule is said to be specific for that target molecule.
For example, a reporter binding molecule with an affinity portion
which is an antibody that binds to a particular antigen is said to
be specific for that antigen. The antigen is the target molecule.
Reporter binding agents are also referred to herein as reporter
binding molecules. FIGS. 25, 26, 27, 28, and 29 of U.S. Pat. No.
6,143,495 illustrate examples of several preferred types of
reporter binding molecules and their use. FIG. 29 of U.S. Pat. No.
6,143,495 illustrates a reporter binding molecule using an antibody
as the affinity portion.
[0085] Preferred target molecules are proteins and peptides. Use of
reporter binding agents that target proteins and peptides allows
sensitive signal amplification using rolling circle amplification
for the detection of proteins and peptides. The ability to
multiplex rolling circle amplification detection allows multiplex
detection of the proteins and peptides (or any other target
molecule). Thus, the disclosed method can be used for multi-protein
analysis such as proteomics analysis. Such multi-protein analysis
can be accomplished, for example, by using reporter binding agents
targeted to different proteins, with the oligonucleotide portion of
each reporter binding agent coded to allow separate amplification
and detection of each different reporter binding agent.
[0086] In one embodiment, the oligonucleotide portion of a reporter
binding agent includes a sequence, referred to as a target
sequence, that serves as a target sequence for an OCP. The sequence
of the target sequence can be arbitrarily chosen. In a multiplex
assay using multiple reporter binding agents, it is preferred that
the target sequence for each reporter binding agent be
substantially different to limit the possibility of non-specific
target detection. Alternatively, it may be desirable in some
multiplex assays, to use target sequences with related sequences.
By using different, unique gap oligonucleotides to fill different
gap spaces, such assays can use one or a few OCPs to amplify and
detect a larger number of target sequences. The oligonucleotide
portion can be coupled to the affinity portion by any of several
established coupling reactions. For example, Hendrickson et al.,
Nucleic Acids Res., 23(3):522-529 (1995) describes a suitable
method for coupling oligonucleotides to antibodies.
[0087] A preferred form of target sequence in a reporter binding
agent is an oligonucleotide having both ends coupled to the
specific binding molecule so as to form a loop. In this way, when
the OCP hybridizes to the target and is circularized, the OCP will
remain topologically locked to the reporter binding agent during
rolling circle replication of the circularized OCP. This improves
the localization of the resulting amplified signal to the location
where the reporter binding agent is bound (that is, at the location
of the target molecule).
[0088] A special form of reporter binding molecule, referred to
herein as a reporter binding probe, has an oligonucleotide or
oligonucleotide derivative as the specific binding molecule.
Reporter binding probes are designed for and used to detect
specific nucleic acid sequences. Thus, the target molecule for
reporter binding probes are nucleic acid sequences. The target
molecule for a reporter binding probe can be a nucleotide sequence
within a larger nucleic acid molecule. It is to be understood that
the term reporter binding molecule encompasses reporter binding
probes. The specific binding molecule of a reporter binding probe
can be any length that supports specific and stable hybridization
between the reporter binding probe and the target molecule. For
this purpose, a length of 10 to 40 nucleotides is preferred, with a
specific binding molecule of a reporter binding probe 16 to 25
nucleotides long being most preferred. It is preferred that the
specific binding molecule of a reporter binding probe is peptide
nucleic acid. As described above, peptide nucleic acid forms a
stable hybrid with DNA. This allows a reporter binding probe with a
peptide nucleic acid specific binding molecule to remain firmly
adhered to the target sequence during subsequent amplification and
detection operations. This useful effect can also be obtained with
reporter binding probes with oligonucleotide specific binding
molecules by making use of the triple helix chemical bonding
technology described by Gasparro et al., Nucleic Acids Res. 1994
22(14):2845-2852 (1994). Briefly, the affinity portion of a
reporter binding probe is designed to form a triple helix when
hybridized to a target sequence. This is accomplished generally as
known, preferably by selecting either a primarily homopurine or
primarily homopyrimidine target sequence. The matching
oligonucleotide sequence which constitutes the affinity portion of
the reporter binding probe will be complementary to the selected
target sequence and thus be primarily homopyrimidine or primarily
homopurine, respectively. The reporter binding probe (corresponding
to the triple helix probe described by Gasparro et al.) contains a
chemically linked psoralen derivative. Upon hybridization of the
reporter binding probe to a target sequence, a triple helix forms.
By exposing the triple helix to low wavelength ultraviolet
radiation, the psoralen derivative mediates cross-linking of the
probe to the target sequence. FIGS. 25, 26, 27, and 28 of U.S. Pat.
No. 6,143,495 illustrate examples of reporter binding molecules
that are reporter binding probes.
[0089] The specific binding molecule in a reporter binding probe
can also be a bipartite DNA molecule, such as ligatable DNA probes
adapted from those described by Landegren et al., Science
241:1077-1080 (1988). When using such a probe, the affinity portion
of the probe is assembled by target-mediated ligation of two
oligonucleotide portions which hybridize to adjacent regions of a
target nucleic acid. Thus, the components used to form the affinity
portion of such reporter binding probes are a truncated reporter
binding probe (with a truncated affinity portion which hybridizes
to part of the target sequence) and a ligation probe which
hybridizes to an adjacent part of the target sequence such that it
can be ligated to the truncated reporter binding probe. The
ligation probe can also be separated from (that is, not adjacent
to) the truncated reporter binding probe when both are hybridized
to the target sequence. The resulting space between them can then
be filled by a second ligation probe or by gap-filling synthesis.
For use in the disclosed methods, it is preferred that the
truncated affinity portion be long enough to allow target-mediated
ligation but short enough to, in the absence of ligation to the
ligation probe, prevent stable hybridization of the truncated
reporter binding probe to the target sequence during the subsequent
amplification operation. For this purpose, a specific step designed
to eliminate hybrids between the target sequence and unligated
truncated reporter binding probes can be used following the
ligation operation.
[0090] In another embodiment, the oligonucleotide portion of a
reporter binding agent includes a sequence, referred to as a
rolling circle replication primer sequence, that serves as a
rolling circle replication primer for an ATC. This allows rolling
circle replication of an added ATC where the resulting TS-DNA is
coupled to the reporter binding agent. Because of this, the TS-DNA
will be effectively immobilized at the site of the target molecule.
Preferably, the immobilized TS-DNA can then be collapsed in situ
prior to detection. The sequence of the rolling circle replication
primer sequence can be arbitrarily chosen. The rolling circle
replication sequence can be designed to form and intramolecular
stem structure as described for rolling circle replication primers
above.
[0091] In a multiplex assay using multiple reporter binding agents,
it is preferred that the rolling circle replication primer sequence
for each reporter binding agent be substantially different to limit
the possibility of non-specific target detection. Alternatively, it
may be desirable in some multiplex assays, to use rolling circle
replication primer sequences with related sequences. Such assays
can use one or a few ATCs to detect a larger number of target
molecules. When the oligonucleotide portion of a reporter binding
agent is used as a rolling circle replication primer, the
oligonucleotide portion can be any length that supports specific
and stable hybridization between the oligonucleotide portion and
the primer complement portion of an amplification target circle.
Generally this is 10 to 35 nucleotides long, but is preferably 16
to 20 nucleotides long. FIGS. 25, 26, 27, 28, and 29 of U.S. Pat.
No. 6,143,495 illustrate examples of reporter binding molecules in
which the oligonucleotide portion is a rolling circle replication
primer.
[0092] In another embodiment, the oligonucleotide portion of a
reporter binding agent can include an amplification target circle
which serves as a template for rolling circle replication. In a
multiplex assay using multiple reporter binding agents, it is
preferred that address tag portions and detection tag portions of
the ATC comprising the oligonucleotide portion of each reporter
binding agent be substantially different to unique detection of
each reporter binding agent. It is desirable, however, to use the
same primer complement portion in all of the ATCs used in a
multiplex assay. The ATC is tethered to the specific binding
molecule by looping the ATC around a tether loop. This allows the
ATC to rotate freely during rolling circle replication while
remaining coupled to the affinity portion. The tether loop can be
any material that can form a loop and be coupled to a specific
binding molecule. Linear polymers are a preferred material for
tether loops.
[0093] A preferred method of producing a reporter binding agent
with a tethered ATC is to form the tether loop by ligating the ends
of oligonucleotides coupled to a specific binding molecule around
an ATC. Oligonucleotides can be coupled to specific binding
molecules using known techniques. For example, Hendrickson et al.
(1995), describes a suitable method for coupling oligonucleotides
to antibodies. This method is generally useful for coupling
oligonucleotides to any protein. To allow ligation,
oligonucleotides comprising the two halves of the tether loop
should be coupled to the specific binding molecule in opposite
orientations such that the free end of one is the 5' end and the
free end of the other is the 3' end. Ligation of the ends of the
tether oligonucleotides can be mediated by hybridization of the
ends of the tether oligonucleotides to adjacent sequences in the
ATC to be tethered. In this way, the ends of the tether
oligonucleotides are analogous to the target probe portions of an
open circle probe, with the ATC containing the target sequence.
Similar techniques can be used to form tether loops containing a
target sequence.
[0094] Another preferred method of producing a reporter binding
agent with a tethered ATC is to ligate an open circle probe while
hybridized to an oligonucleotide tether loop on a specific binding
molecule. In this method, both ends of a single tether
oligonucleotide are coupled to a specific binding molecule. This
can be accomplished using known coupling techniques as described
above. Ligation of an open circle probe hybridized to a tether loop
is analogous to the ligation operation of LM-RCA. In this case, the
target sequence is part of an oligonucleotide with both ends
coupled to a specific binding molecule. This same ligation
technique can be used to circularize open circle probes on target
sequences that are part of reporter binding agents. This
topologically locks the open circle probe to the reporter binding
agent (and thus, to the target molecule to which the reporter
binding agent binds).
[0095] The ends of tether loops can be coupled to any specific
binding molecule with functional groups that can be derivatized
with suitable activating groups. When the specific binding molecule
is a protein, or a molecule with similar functional groups,
coupling of tether ends can be accomplished using known methods of
protein attachment. Many such methods are described in Protein
immobilization: fundamentals and applications Richard F. Taylor,
ed. (M. Dekker, New York, 1991).
[0096] Antibodies useful as the affinity portion of reporter
binding agents, can be obtained commercially or produced using well
established methods. For example, Johnstone and Thorpe, on pages
30-85, describe general methods useful for producing both
polyclonal and monoclonal antibodies. The entire book describes
many general techniques and principles for the use of antibodies in
assay systems.
[0097] G. Detection Labels
[0098] To aid in detection and quantitation of nucleic acids
amplified using RCA and RCT, detection labels can be directly
incorporated into amplified nucleic acids or can be coupled to
detection molecules. As used herein, a detection label is any
molecule that can be associated with amplified nucleic acid,
directly or indirectly, and which results in a measurable,
detectable signal, either directly or indirectly. Many such labels
for incorporation into nucleic acids or coupling to nucleic acid or
antibody probes are known to those of skill in the art. Examples of
detection labels suitable for use in RCA and RCT are radioactive
isotopes, fluorescent molecules, phosphorescent molecules, enzymes,
antibodies, and ligands.
[0099] Examples of suitable fluorescent labels include fluorescein
(FITC), 5,6-carboxymethyl fluorescein, Texas red,
nitrobenz-2-oxa-1,3-diazol-4-yl (NBD), coumarin, dansyl chloride,
rhodamine, 4'-6-diamidino-2-phenylinodo- le (DAPI), and the cyanine
dyes Cy3, Cy3.5, Cy5, Cy5.5 and Cy7. Preferred fluorescent labels
are fluorescein (5-carboxyfluorescein-N-hydroxysuccini- mide ester)
and rhodamine (5,6-tetramethyl rhodamine). Preferred fluorescent
labels for combinatorial multicolor coding are FITC and the cyanine
dyes Cy3, Cy3.5, Cy5, Cy5.5 and Cy7. The absorption and emission
maxima, respectively, for these fluors are: FITC (490 nm; 520 nm),
Cy3 (554 nm; 568 nm), Cy3.5 (581 nm; 588 nm), Cy5 (652 nm: 672 nm),
Cy5.5 (682 nm; 703 nm) and Cy7 (755 nm; 778 nm), thus allowing
their simultaneous detection. The fluorescent labels can be
obtained from a variety of commercial sources, including Molecular
Probes, Eugene, Oreg. and Research Organics, Cleveland, Ohio.
[0100] Labeled nucleotides are preferred form of detection label
since they can be directly incorporated into the products of RCA
and RCT during synthesis. Examples of detection labels that can be
incorporated into amplified DNA or RNA include nucleotide analogs
such as BrdUrd (Hoy and Schimke, Mutation Research 290:217-230
(1993)), BrUTP (Wansick et al., J. Cell Biology 122:283-293 (1993))
and nucleotides modified with biotin (Langer et al., Proc. Natl.
Acad. Sci. USA 78:6633 (1981)) or with suitable haptens such as
digoxygenin (Kerkhof, Anal. Biochem. 205:359-364 (1992)). Suitable
fluorescence-labeled nucleotides are
Fluorescein-isothiocyanate-dUTP, Cyanine-3-dUTP and Cyanine-5-dUTP
(Yu et al., Nucleic Acids Res., 22:3226-3232 (1994)). A preferred
nucleotide analog detection label for DNA is BrdUrd (BUDR
triphosphate, Sigma), and a preferred nucleotide analog detection
label for RNA is Biotin-16-uridine-5' -triphosphate
(Biotin-16-dUTP, Boehringher Mannheim). Fluorescein, Cy3, and Cy5
can be linked to dUTP for direct labeling. Cy3.5 and Cy7 are
available as avidin or anti-digoxygenin conjugates for secondary
detection of biotin- or digoxygenin-labeled probes.
[0101] Detection labels that are incorporated into amplified
nucleic acid, such as biotin, can be subsequently detected using
sensitive methods well-known in the art. For example, biotin can be
detected using streptavidin-alkaline phosphatase conjugate (Tropix,
Inc.), which is bound to the biotin and subsequently detected by
chemiluminescence of suitable substrates (for example,
chemiluminescent substrate CSPD: disodium,
3-(4-methoxyspiro-[1,2,-dioxetane-3-2'-(5'-chloro)tricyclo
[3.3.1.1.sup.3,7]decanel-4-yl) phenyl phosphate; Tropix, Inc.).
[0102] A preferred detection label for use in detection of
amplified RNA is acridinium-ester-labeled DNA probe (GenProbe,
Inc., as described by Arnold et al., Clinical Chemistry
35:1588-1594 (1989)). An acridinium-ester-labeled detection probe
permits the detection of amplified RNA without washing because
unhybridized probe can be destroyed with alkali (Arnold et al.
(1989)).
[0103] Molecules that combine two or more of these detection labels
are also considered detection labels. Any of the known detection
labels can be used with the disclosed probes, tags, and method to
label and detect nucleic acid amplified using the disclosed method.
Methods for detecting and measuring signals generated by detection
labels are also known to those of skill in the art. For example,
radioactive isotopes can be detected by scintillation counting or
direct visualization; fluorescent molecules can be detected with
fluorescent spectrophotometers; phosphorescent molecules can be
detected with a spectrophotometer or directly visualized with a
camera; enzymes can be detected by detection or visualization of
the product of a reaction catalyzed by the enzyme; antibodies can
be detected by detecting a secondary detection label coupled to the
antibody. Such methods can be used directly in the disclosed method
of amplification and detection. As used herein, detection molecules
are molecules that interact with amplified nucleic acid and to
which one or more detection labels are coupled.
[0104] H. Detection Probes
[0105] Detection probes-are labeled oligonucleotides having
sequence complementary to detection tags on TS-DNA or transcripts
of TS-DNA. The complementary portion of a detection probe can be
any length that supports specific and stable hybridization between
the detection probe and the detection tag. For this purpose, a
length of 10 to 35 nucleotides is preferred, with a complementary
portion of a detection probe 16 to 20 nucleotides long being most
preferred. Detection probes can contain any of the detection labels
described above. Preferred labels are biotin and fluorescent
molecules. A particularly preferred detection probe is a molecular
beacon. Molecular beacons are detection probes labeled with
fluorescent moieties where the fluorescent moieties fluoresce only
when the detection probe is hybridized (Tyagi and Kramer, Nature
Biotechnology 14:303-308 (1996)). The use of such probes eliminates
the need for removal of unhybridized probes prior to label
detection because the unhybridized detection probes will not
produce a signal. This is especially useful in multiplex
assays.
[0106] A preferred form of detection probe, referred to herein as a
collapsing detection probe, contains two separate complementary
portions. This allows each detection probe to hybridize to two
detection tags in TS-DNA. In this way, the detection probe forms a
bridge between different parts of the TS-DNA. The combined action
of numerous collapsing detection probes hybridizing to TS-DNA will
be to form a collapsed network of cross-linked TS-DNA. Collapsed
TS-DNA occupies a much smaller volume than free, extended TS-DNA,
and includes whatever detection label present on the detection
probe. This result is a compact and discrete detectable signal for
each TS-DNA. Collapsing TS-DNA is useful both for in situ
hybridization applications and for multiplex detection because it
allows detectable signals to be spatially separate even when
closely packed. Collapsing TS-DNA is especially preferred for use
with combinatorial multicolor coding.
[0107] TS-DNA collapse can also be accomplished through the use of
ligand/ligand binding pairs (such as biotin and avidin) or
hapten/antibody pairs. As described in U.S. Pat. No. 6,143,495
(Example 6), a nucleotide analog, BUDR, can be incorporated into
TS-DNA during rolling circle replication. When biotinylated
antibodies specific for BUDR and avidin are added, a cross-linked
network of TS-DNA forms, bridged by avidin-biotin-antibody
conjugates, and the TS-DNA collapses into a compact structure.
Collapsing detection probes and biotin-mediated collapse can also
be used together to collapse TS-DNA.
[0108] I. Address Probes
[0109] An address probe is an oligonucleotide having a sequence
complementary to address tags on TS-DNA or transcripts of TS-DNA.
The complementary portion of an address probe can be any length
that supports specific and stable hybridization between the address
probe and the address tag. For this purpose, a length of 10 to 35
nucleotides is preferred, with a complementary portion of an
address probe 12 to 18 nucleotides long being most preferred.
Preferably, the complementary portion of an address probe is
complementary to all or a portion of the target probe portions of
an OCP. Most preferably, the complementary portion of an address
probe is complementary to a portion of either or both of the left
and right target probe portions of an OCP and all or a part of any
gap oligonucleotides or gap sequence created in a gap-filling
operation (see FIG. 6 of U.S. Pat. No. 6,143,495). Address probe
can contain a single complementary portion or multiple
complementary portions. Preferably, address probes are coupled,
either directly or via a spacer molecule, to a solid-state support.
Such a combination of address probe and solid-state support are a
preferred form of solid-state detector.
[0110] J. Oligonucleotide Synthesis
[0111] Open circle probes, gap oligonucleotides, rolling circle
replication primers, detection probes, address probes,
amplification target circles, DNA strand displacement primers, and
any other oligonucleotides can be synthesized using established
oligonucleotide synthesis methods. Methods to produce or synthesize
oligonucleotides are well known. Such methods can range from
standard enzymatic digestion followed by nucleotide fragment
isolation (see for example, Sambrook et al., Molecular Cloning: A
Laboratory Manual, 2nd Edition (Cold Spring Harbor Laboratory
Press, Cold Spring Harbor, N.Y., 1989) Chapters 5, 6) to purely
synthetic methods, for example, by the cyanoethyl phosphoramidite
method using a Milligen or Beckman System 1Plus DNA synthesizer
(for example, Model 8700 automated synthesizer of
Milligen-Biosearch, Burlington, Mass. or ABI Model 380B). Synthetic
methods useful for making oligonucleotides are also described by
Ikuta et al., Ann. Rev. Biochem. 53:323-356 (1984),
(phosphotriester and phosphite-triester methods), and Narang et
al., Methods Enzymol., 65:610-620 (1980), (phosphotriester method).
Protein nucleic acid molecules can be made using known methods such
as those described by Nielsen et al., Bioconjug. Chem. 5:3-7
(1994).
[0112] Many of the oligonucleotides described herein are designed
to be complementary to certain portions of other oligonucleotides
or nucleic acids such that stable hybrids can be formed between
them. The stability of these hybrids can be calculated using known
methods such as those described in Lesnick and Freier, Biochemistry
34:10807-10815 (1995), McGraw et al., Biotechniques 8:674-678
(1990), and Rychlik et al., Nucleic Acids Res. 18:6409-6412
(1990).
[0113] K. Solid-State Detectors
[0114] Solid-state detectors are solid-state substrates or supports
to which address probes or detection molecules have been coupled. A
preferred form of solid-state detector is an array detector. An
array detector is a solid-state detector to which multiple
different address probes or detection molecules have been coupled
in an array, grid, or other organized pattern.
[0115] Solid-state substrates for use in solid-state detectors can
include any solid material to which oligonucleotides can be
coupled. This includes materials such as acrylamide, cellulose,
nitrocellulose, glass, polystyrene, polyethylene vinyl acetate,
polypropylene, polymethacrylate, polyethylene, polyethylene oxide,
glass, polysilicates, polycarbonates, teflon, fluorocarbons, nylon,
silicon rubber, polyanhydrides, polyglycolic acid, polylactic acid,
polyorthoesters, polypropylfumerate, collagen, glycosaminoglycans,
and polyamino acids. Solid-state substrates can have any useful
form including thin films or membranes, beads, bottles, dishes,
fibers, woven fibers, shaped polymers, particles and
microparticles. A preferred form for a solid-state substrate is a
microtiter dish.
[0116] Address probes immobilized on a solid-state substrate allow
capture of the products of RCA and RCT on a solid-state detector.
Such capture provides a convenient means of washing away reaction
components that might interfere with subsequent detection steps. By
attaching different address probes to different regions of a
solid-state detector, different RCA or RCT products can be captured
at different, and therefore diagnostic, locations on the
solid-state detector. For example, in a microtiter plate multiplex
assay, address probes specific for up to 96 different TS-DNAs (each
amplified via a different target sequence) can be immobilized on a
microtiter plate, each in a different well. Capture and detection
will occur only in those wells corresponding to TS-DNAs for which
the corresponding target sequences were present in a sample.
[0117] Methods for immobilization of oligonucleotides to
solid-state substrates are well established. Oligonucleotides,
including address probes and detection probes, can be coupled to
substrates using established coupling methods. For example,
suitable attachment methods are described by Pease et al., Proc.
Natl. Acad. Sci. USA 91(11):5022-5026 (1994), and Khrapko et al.,
Mol Biol (Mosk) (USSR) 25:718-730 (1991). A method for
immobilization of 3' -amine oligonucleotides on casein-coated
slides is described by Stimpson et al., Proc. Natl. Acad. Sci. USA
92:6379-6383 (1995). A preferred method of attaching
oligonucleotides to solid-state substrates is described by Guo et
al., Nucleic Acids Res. 22:5456-5465 (1994).
[0118] Some solid-state detectors useful in RCA and RCT assays have
detection antibodies attached to a solid-state substrate. Such
antibodies can be specific for a molecule of interest. Captured
molecules of interest can then be detected by binding of a second,
reporter antibody, followed by RCA or RCT. Such a use of antibodies
in a solid-state detector allows RCA assays to be developed for the
detection of any molecule for which antibodies can be generated.
Methods for immobilizing antibodies to solid-state substrates are
well established. Immobilization can be accomplished by attachment,
for example, to aminated surfaces, carboxylated surfaces or
hydroxylated surfaces using standard immobilization chemistries.
Examples of attachment agents are cyanogen bromide, succinimide,
aldehydes, tosyl chloride, avidin-biotin, photocrosslinkable
agents, epoxides and maleimides. A preferred attachment agent is
glutaraldehyde. These and other attachment agents, as well as
methods for their use in attachment, are described in Protein
immobilization: fundamentals and applications, Richard F. Taylor,
ed. (M. Dekker, New York, 1991), Johnstone and Thorpe,
Immunochemistry In Practice (Blackwell Scientific Publications,
Oxford, England, 1987) pages 209-216 and 241-242, and Immobilized
Affinity Ligands, Craig T. Herrnanson et al., eds. (Academic Press,
New York, 1992). Antibodies can be attached to a substrate by
chemically cross-linking a free amino group on the antibody to
reactive side groups present within the solid-state substrate. For
example, antibodies may be chemically cross-linked to a substrate
that contains free amino or carboxyl groups using glutaraldehyde or
carbodiimides as cross-linker agents. In this method, aqueous
solutions containing free antibodies are incubated with the
solid-state substrate in the presence of glutaraldehyde or
carbodiimide. For crosslinking with glutaraldehyde the reactants
can be incubated with 2% glutaraldehyde by volume in a buffered
solution such as 0.1 M sodium cacodylate at pH 7.4. Other standard
immobilization chemistries are known by those of skill in the
art.
[0119] L. Solid-State Samples
[0120] Solid-state samples are solid-state substrates or supports
to which target molecules or target sequences have been coupled or
adhered. Target molecules or target sequences are preferably
delivered in a target sample or assay sample. A preferred form of
solid-state sample is an array sample. An array sample is a
solid-state sample to which multiple different target samples or
assay samples have been coupled or adhered in an array, grid, or
other organized pattern.
[0121] Solid-state substrates for use in solid-state samples can
include any solid material to which target molecules or target
sequences can be coupled or adhered. This includes materials such
as acrylamide, cellulose, nitrocellulose, glass, polystyrene,
polyethylene vinyl acetate, polypropylene, polymethacrylate,
polyethylene, polyethylene oxide, glass, polysilicates,
polycarbonates, teflon, fluorocarbons, nylon, silicon rubber,
polyanhydrides, polyglycolic acid, polylactic acid,
polyorthoesters, polypropylfumerate, collagen, glycosaminoglycans,
and polyamino acids. Solid-state substrates can have any useful
form including thin films or membranes, beads, bottles, dishes,
slides, fibers, woven fibers, shaped polymers, particles and
microparticles. Preferred forms for a solid-state substrate are
microtiter dishes and glass slides.
[0122] Target molecules and target sequences immobilized on a
solid-state substrate allow formation of target-specific TS-DNA
localized on the solid-state substrate. Such localization provides
a convenient means of washing away reaction components that might
interfere with subsequent detection steps, and a convenient way of
assaying multiple different samples simultaneously. Diagnostic
TS-DNA can be independently formed at each site where a different
sample is adhered. For immobilization of target sequences or other
oligonucleotide molecules to form a solid-state sample, the methods
described above for can be used. Where the target molecule is a
protein or peptide, the protein or peptide can be immobilized on a
solid-state substrate generally as described above for the
immobilization of antibodies. Proteins and peptides within target
samples are preferred targets of detection on solid-state
substrates. Such an arrangement is a preferred platform for
proteomics analysis using the disclosed materials and method.
[0123] A preferred form of solid-state substrate is a glass slide
to which up to 256 separate target or assay samples have been
adhered as an array of small dots. Each dot is preferably from 0.1
to 2.5 mm in diameter, and most preferably around 2.5 mm in
diameter. Such microarrays can be fabricated, for example, using
the method described by Schena et al., Science 270:487-470 (1995).
Briefly, microarrays can be fabricated on poly-L-lysine-coated
microscope slides (Sigma) with an arraying machine fitted with one
printing tip. The tip is loaded with 1 .mu.l of a DNA sample (0.5
mg/ml) from, for example, 96-well microtiter plates and deposited
.about.0.005 .mu.l per slide on multiple slides at the desired
spacing. The printed slides can then be rehydrated for 2 hours in a
humid chamber, snap-dried at 100.degree. C. for 1 minute, rinsed in
0.1% SDS, and treated with 0.05% succinic anhydride prepared in
buffer consisting of 50% 1-methyl-2-pyrrolidinone and 50% boric
acid. The DNA on the slides can then be denatured in, for example,
distilled water for 2 minutes at 90.degree. C. immediately before
use. Microarray solid-state samples can scanned with, for example,
a laser fluorescent scanner with a computer-controlled XY stage and
a microscope objective. A mixed gas, multiline laser allows
sequential excitation of multiple fluorophores.
[0124] M. DNA Ligases
[0125] Any DNA ligase is suitable for use in the disclosed
amplification method. Preferred ligases are those that
preferentially form phosphodiester bonds at nicks in
double-stranded DNA. That is, ligases that fail to ligate the free
ends of single-stranded DNA at a significant rate are preferred.
Thermostable ligases are especially preferred. Many suitable
ligases are known, such as T4 DNA ligase (Davis et al., Advanced
Bacterial Genetics--A Manual for Genetic Engineering (Cold Spring
Harbor Laboratory, Cold Spring Harbor, N.Y., 1980)), E. coli DNA
ligase (Panasnko et al., J. Biol. Chem. 253:4590-4592 (1978)),
AMPLIGASE.RTM. (Kalin et al., Mutat. Res., 283(2): 119-123 (1992);
Winn-Deen et al., Mol Cell Probes (England) 7(3):179-186 (1993)),
Taq DNA ligase (Barany, Proc. Natl. Acad. Sci. USA 88:189-193
(1991), Thermus thermophilus DNA ligase (Abbott Laboratories),
Thermus scotoductus DNA ligase and Rhodothernius marinus DNA ligase
(Thorbjarnardottir et al., Gene 151:177-180 (1995)). T4 DNA ligase
is preferred for ligations involving RNA target sequences due to
its ability to ligate DNA ends involved in DNA:RNA hybrids (Hsuih
et al., Quantitative detection of HCV RNA using novel
ligation-dependent polymerase chain reaction, American Association
for the Study of Liver Diseases (Chicago, Ill., Nov. 3-7,
1995)).
[0126] The frequency of non-target-directed ligation catalyzed by a
ligase can be determined as follows. LM-RCA is performed with an
open circle probe and a gap oligonucleotide in the presence of a
target sequence. Non-targeted-directed ligation products can then
be detected by using an address probe specific for the open circle
probe ligated without the gap oligonucleotide to capture TS-DNA
from such ligated probes. Target directed ligation products can be
detected by using an address probe specific for the open circle
probe ligated with the gap oligonucleotide. By using a solid-state
detector with regions containing each of these address probes, both
target directed and non-target-directed ligation products can be
detected and quantitated. The ratio of target-directed and
non-target-directed TS-DNA produced provides a measure of the
specificity of the ligation operation. Target-directed ligation can
also be assessed as discussed in Barany (1991).
[0127] N. DNA Polymerases
[0128] DNA polymerases useful in the rolling circle replication
step of the disclosed method must perform rolling circle
replication of primed single-stranded circles. Such polymerases are
referred to herein as rolling circle DNA polymerases. For rolling
circle replication, it is preferred that a DNA polymerase be
capable of displacing the strand complementary to the template
strand, termed strand displacement, and lack a 5' to 3' exonuclease
activity. Strand displacement is necessary to result in synthesis
of multiple tandem copies of the ligated OCP. A 5' to 3'
exonuclease activity, if present, might result in the destruction
of the synthesized strand. DNA polymerases for use in the disclosed
method can also be highly processive, if desired. The suitability
of a DNA polymerase for use in the disclosed method can be readily
determined by assessing its ability to carry out rolling circle
replication. Preferred rolling circle DNA polymerases are Bst DNA
polymerase, VENT.RTM. DNA polymerase (Kong et al., J. Biol. Chem.
268:1965-1975 (1993)), ThermoSequenase.TM., delta Tts DNA
polymerase, bacteriophage .phi.29 DNA polymerase (U.S. Pat. Nos.
5,198,543 and 5,001,050 to Blanco et al.), phage M2 DNA polymerase
(Matsumoto et al., Gene 84:247 (1989)), phage .phi.PRD1 DNA
polymerase (Jung et al., Proc. Natl. Acad. Sci. USA 84:8287
(1987)), Klenow fragment of DNA polymerase I (Jacobsen et al., Eur.
J. Biochem. 45:623-627 (1974)), T5 DNA polymerase (Chatterjee et
al., Gene 97:13-19 (1991)), PRD1 DNA polymerase (Zhu and Ito,
Biochim. Biophys. Acta. 1219:267-276 (1994)), modified T7 DNA
polymerase (Tabor and Richardson, J. Biol. Chem. 262:15330-15333
(1987); Tabor and Richardson, J. Biol. Chem. 264:6447-6458 (1989);
Sequenase.TM. (U.S. Biochemicals)), and T4 DNA polymerase
holoenzyme (Kaboord and Benkovic, Curr. Biol. 5:149-157 (1995)).
More preferred are Bst DNA polymerase, VENT.RTM. DNA polymerase,
ThermoSequenase.TM. , and delta Tts DNA polymerase. Bst DNA
polymerase is most preferred.
[0129] Strand displacement can be facilitated through the use of a
strand displacement factor, such as helicase. It is considered that
any DNA polymerase that can perform rolling circle replication in
the presence of a strand displacement factor is suitable for use in
the disclosed method, even if the DNA polymerase does not perform
rolling circle replication in the absence of such a factor. Strand
displacement factors useful in the disclosed method include BMRF1
polymerase accessory subunit (Tsurumi et al., J. Virology
67(12):7648-7653 (1993)), adenovirus DNA-binding protein
(Zijderveld and van der Vliet, J. Virology 68(2):1158-1164 (1994)),
herpes simplex viral protein ICP8 (Boehmer and Lehman, J. Virology
67(2):711-715 (1993); Skaliter and Lehman, Proc. Natl. Acad. Sci.
USA 91(22):10665-10669 (1994)), single-stranded DNA binding
proteins (SSB; Rigler and Romano, J. Biol. Chem. 270:8910-8919
(1995)), and calf thymus helicase (Siegel et al., J. Biol. Chem.
267:13629-13635 (1992)).
[0130] The ability of a polymerase to carry out rolling circle
replication can be determined by using the polymerase in a rolling
circle replication assay such as those described in Fire and Xu,
Proc. Natl. Acad. Sci. USA 92:4641-4645 (1995) and in U.S. Pat. No.
6,143,495 (Example 1).
[0131] Another type of DNA polymerase can be used if a gap-filling
synthesis step is used, such as in gap-filling LM-RCA (see U.S.
Pat. No. 6,143,495, Example 3). When using a DNA polymerase to fill
gaps, strand displacement by the DNA polymerase is undesirable.
Such DNA polymerases are referred to herein as gap-filling DNA
polymerases. Unless otherwise indicated, a DNA polymerase referred
to herein without specifying it as a rolling circle DNA polymerase
or a gap-filling DNA polymerase, is understood to be a rolling
circle DNA polymerase and not a gap-filling DNA polymerase.
Preferred gap-filling DNA polymerases are T7 DNA polymerase
(Studier et al., Methods Enzymol. 185:60-89 (1990)), DEEP VENT.RTM.
DNA polymerase (New England Biolabs, Beverly, Mass.), modified T7
DNA polymerase (Tabor and Richardson, J. Biol. Chem.
262:15330-15333 (1987); Tabor and Richardson, J. Biol. Chem.
264:6447-6458 (1989); Sequenase.TM. (U.S. Biochemicals)), and T4
DNA polymerase (Kunkel et al., Methods Enzymol. 154:367-382
(1987)). An especially preferred type of gap-filling DNA polymerase
is the Thermus flavus DNA polymerase (MBR, Milwaukee, Wis.). The
most preferred gap-filling DNA polymerase is the Stoffel fragment
of Taq DNA polymerase (Lawyer et al., PCR Methods Appl.
2(4):275-287 (1993), King et al., J. Biol. Chem.
269(18):13061-13064 (1994)).
[0132] The ability of a polymerase to fill gaps can be determined
by performing gap-filling LM-RCA. Gap-filling LM-RCA is performed
with an open circle probe that forms a gap space when hybridized to
the target sequence. Ligation can only occur when the gap space is
filled by the DNA polymerase. If gap-filling occurs, TS-DNA can be
detected, otherwise it can be concluded that the DNA polymerase, or
the reaction conditions, is not useful as a gap-filling DNA
polymerase.
[0133] O. RNA Polymerases
[0134] Any RNA polymerase which can carry out transcription in
vitro and for which promoter sequences have been identified can be
used in the disclosed rolling circle transcription method. Stable
RNA polymerases without complex requirements are preferred. Most
preferred are T7 RNA polymerase (Davanloo et al., Proc. Natl. Acad.
Sci. USA 81:2035-2039 (1984)) and SP6 RNA polymerase (Butler and
Chamberlin, J. Biol. Chem. 257:5772-5778 (1982)) which are highly
specific for particular promoter sequences (Schenborn and
Meirendorf, Nucleic Acids Research 13:6223-6236 (1985)). Other RNA
polymerases with this characteristic are also preferred. Because
promoter sequences are generally recognized by specific RNA
polymerases, the OCP or ATC should contain a promoter sequence
recognized by the RNA polymerase that is used. Numerous promoter
sequences are known and any suitable RNA polymerase having an
identified promoter sequence can be used. Promoter sequences for
RNA polymerases can be identified using established techniques.
[0135] P. Kits
[0136] The materials described above can be packaged together in
any suitable combination as a kit useful for performing the
disclosed method. It is preferred that the kit components in a
given kit be designed and adapted for use together in the disclosed
method. A kit can include one or more open circle probes and one or
more rolling circle replication primers. A kit can also include a
secondary DNA strand displacement primer, a tertiary DNA strand
displacement primer, or both. A kit can also include one or more
gap oligonucleotides. The target probe portions of the open circle
probes in a kit preferably are each complementary to a different
target sequence. A kit can also include one or more detection
probes. Preferably, a portion of each of the detection probes in a
kit has sequence matching or complementary to a portion of a
different one of the open circle probes in that kit.
[0137] A kit can also include one or more reporter binding agents
where the oligonucleotide portion of the reporter binding agents
include one of the target sequences. The specific binding molecules
of the reporter binding agents in a kit each can be specific for an
analyte, preferably specific for a protein or peptide.
[0138] A preferred kit for selectively detecting one or more target
sequences or selectively amplifying nucleic acid sequences related
to one or more target sequences can include a plurality of open
circle probes, one or more rolling circle replication primers, and
a plurality of detection probes. In this kit, it is preferred that
at least one of the ends of one of the open circle probe can form
an intramolecular stem structure, and that portions of each open
circle probe are complementary to one or more target sequences such
that the portions of the open circle probes that are complementary
to the target sequence are complementary to a different target
sequence for each of a plurality of the open circle probes. It is
also preferred that all or a portion of each rolling circle
replication primer is complementary to a portion of one or more of
the open circle probes, and that a portion of each of the detection
probes has sequence matching or complementary to a portion of a
different one of the open circle probes.
[0139] Another preferred kit for selectively detecting one or more
target sequences or selectively amplifying nucleic acid sequences
related to one or more target sequences can include a plurality of
open circle probes, one or more rolling circle replication primers,
and a plurality of reporter binding agents. In this kit, it is
preferred that at least one of the ends of one of the open circle
probe can form an intramolecular stem structure, and that portions
of each open circle probe are complementary to one or more target
sequences such that the portions of the open circle probes that are
complementary to the target sequence are complementary to a
different target sequence for each of a plurality of the open
circle probes. It is also preferred that all or a portion of each
rolling circle replication primer is complementary to a portion of
one or more of the open circle probes, and that the oligonucleotide
portion of each reporter binding agent include target sequences. It
is also preferred that the reporter binding agent s are specific
for proteins or peptides. This kit can also include a plurality of
detection probes, preferably where a portion of each of the
detection probes has sequence matching or complementary to a
portion of a different one of the open circle probes.
[0140] Method
[0141] The disclosed method involves the use of hairpin open circle
probes, hairpin rolling circle replication primers, hairpin DNA
strand displacement primers, or a combination, in a rolling circle
amplification technique. For use of hairpin open circle probes, the
basic method involves a ligation operation and an amplification
operation. The ligation operation involves circularization of the
open circle probe and is mediated by a target sequence to which the
open circle probe is hybridized. A key feature of this operation is
connection between an open circle probe and its cognate target
sequence. Unless the target sequence is present (so that the open
circle probe can hybridize to it), the open circle probe will not
be circularized. The disclosed hairpin open circle probes can
improve the specificity of open circle probe circularization by
improving the specificity of hybridization.
[0142] The amplification operation involves rolling circle
replication of the circularized open circle probe. The disclosed
hairpin open circle probes can improve the amplification operation
by reducing or eliminating non-specific priming by uncircularized
open circle probes. Such non-specific priming can result in
amplification of non-specific sequences, thus reducing the
specificity of the amplification operation. The amplification
operation uses rolling circle replication primers to prime
replication of circularized open circle probes. The disclosed
hairpin rolling circle replication primers can improve the
amplification operation by reducing or eliminating non-specific
priming by the rolling circle replication primers.
[0143] The amplification operation can also include replication of
the product of rolling circle replication (referred to as secondary
DNA strand displacement). This involves priming and replication of
the tandem repeat DNA produced by rolling circle replication using
secondary DNA strand displacement primers. The disclosed hairpin
secondary DNA strand displacement primers can improve the
amplification operation by reducing or eliminating non-specific
priming by the secondary DNA strand displacement primers. The
amplification operation can also include replication of the product
of secondary DNA strand displacement (referred to as tertiary DNA
strand displacement). This involves priming and replication of the
tandem repeat DNA produced by secondary DNA strand displacement
using tertiary DNA strand displacement primers. The disclosed
hairpin tertiary DNA strand displacement primers can improve the
amplification operation by reducing or eliminating non-specific
priming by the tertiary DNA strand displacement primers.
[0144] The disclosed method is useful for detection, quantitation,
and/or location of any desired analyte. The disclosed method can be
multiplexed to detect numerous different analytes simultaneously or
used in a single assay. Thus, the disclosed method is useful for
detecting, assessing, quantitating, profiling, and/or cataloging
gene expression and the presence of protein in biological samples.
The disclosed method is also particularly useful for detecting and
discriminating single nucleotide differences in nucleic acid
sequences. This specificity is possible due to the sensitivity of
the intramolecular stem structure in loop-containing probes and
primers to mismatches between the loop sequence and a prospective
target sequence. Thus, the disclosed method is useful for extensive
multiplexing of target sequences for sensitive and specific
detection of the target sequences themselves or analytes to which
the target sequences have been associated. The disclosed method is
also useful for detecting, assessing, quantitating, and/or
cataloging single nucleotide polymorphisms, and other sequence
differences between nucleic acids, nucleic acid samples, and
sources of nucleic acid samples.
[0145] The disclosed method is useful for detecting any desired
sequence or other analyte, such as proteins and peptides. In
particular, the disclosed method can be used to localize or amplify
signal from any desired analyte. For example, the disclosed method
can be used to assay tissue, transgenic cells, bacterial or yeast
colonies, cellular material (for example, whole cells, proteins,
DNA fibers, interphase nuclei, or metaphase chromosomes on slides,
arrayed genomic DNA, RNA), and samples and extracts from any of
biological source. Where target sequences are associated with an
analyte, different target sequences, and thus different analytes,
can be sensitively distinguished. Specificity of such detection is
aided by sensitivity of a loop in an open circle probe to
mismatches.
[0146] The disclosed method is applicable to numerous areas
including, but not limited to, analysis of proteins present in a
sample (for example, proteomics analysis), disease detection,
mutation detection, protein expression profiling, RNA expression
profiling, gene discovery, gene mapping (molecular haplotyping),
agricultural research, and virus detection. Preferred uses include
protein and peptide detection in situ in cells, on microarrays,
protein expression profiling; mutation detection; detection of
abnormal proteins or peptides (for example, overexpression of an
oncogene protein or absence of expression of a tumor suppressor
protein); expression in cancer cells; detection of viral proteins
in cells; viral protein expression; detection of inherited diseases
such as cystic fibrosis, muscular dystrophy, diabetes, hemophilia,
sickle cell anemia; assessment of predisposition for cancers such
as prostate cancer, breast cancer, lung cancer, colon cancer,
ovarian cancer, testicular cancer, pancreatic cancer. The disclosed
method can also be used for detection of nucleic acids in situ in
cells, on microarrays, on DNA fibers, and on genomic DNA arrays;
detection of RNA in cells; RNA expression profiling; molecular
haplotyping; mutation detection; detection of abnormal RNA (for
example, overexpression of an oncogene or absence of expression of
a tumor suppressor gene); expression in cancer cells; detection of
viral genome in cells; viral RNA expression; detection of inherited
diseases such as cystic fibrosis, muscular dystrophy, diabetes,
hemophilia, sickle cell anemia; assessment of predisposition for
cancers such as prostate cancer, breast cancer, lung cancer, colon
cancer, ovarian cancer, testicular cancer, pancreatic cancer.
[0147] A. The Ligation Operation
[0148] An open circle probe, optionally in the presence of one or
more gap oligonucleotides, is incubated with a sample containing
nucleic acids, under suitable hybridization conditions, and then
ligated to form a covalently closed circle. The ligated open circle
probe is a form of amplification target circle. This operation is
similar to ligation of padlock probes described by Nilsson et al.,
Science, 265:2085-2088 (1994). The ligation operation allows
subsequent amplification to be dependent on the presence of a
target sequence. Suitable ligases for the ligation operation are
described above. Ligation conditions are generally known. Most
ligases require Mg.sup.++. There are two main types of ligases,
those that are ATP-dependent and those that are NAD-dependent. ATP
or NAD, depending on the type of ligase, should be present during
ligation.
[0149] The disclosed hairpin open circle probes reduce the
incidence of non-specific ligation of open circle probe ends
because one or both of the ends remain in the intramolecular stem
structure unless hybridized to a target sequence. Loop-containing
open circle probes allow better discrimination of target sequence
hybridization by the open circle probes. As discussed below,
hybridization of sequences in the loop to target sequence can
disrupt the intramolecular stem structure. In the absence of target
sequence, the stem structure remains intact.
[0150] The target sequence for an open circle probe can be any
nucleic acid or other compound to which the target probe portions
of the open circle probe can hybridize in the proper alignment.
Target sequences can be found in any nucleic acid molecule from any
nucleic acid sample. Thus, target sequences can be in nucleic acids
in cell or tissue samples, reactions, and assays. Target sequences
can also be artificial nucleic acids (or other compounds to which
the target probe portions of the open circle probe can hybridize in
the proper alignment). For example, nucleic acid tags can be
associated with various of the disclosed compounds to be detected
using open circle probes. Thus, a reporter binding agent can
contain a target sequence to which an open circle probe can
hybridize. In these cases, the target sequence provides a link
between the target molecule being detected and the amplification of
signal mediated by the open circle probe.
[0151] When RNA is to be detected, it is preferred that a reverse
transcription operation be performed to make a DNA target sequence.
Alternatively, an RNA target sequence can be detected directly by
using a ligase that can perform ligation on a DNA:RNA hybrid
substrate. A preferred ligase for this is T4 DNA ligase.
[0152] B. The Amplification Operation
[0153] The basic form of amplification operation is rolling circle
replication of a circular DNA molecule (that is, a circularized
open circle probe or an amplification target circle). The circular
open circle probes formed by specific ligation and amplification
target circles serve as substrates for a rolling circle
replication. This reaction requires two reagents: (a) a rolling
circle replication primer, which is complementary to the primer
complement portion of the OCP or ATC, and (b) a rolling circle DNA
polymerase. The DNA polymerase catalyzes primer extension and
strand displacement in a processive rolling circle polymerization
reaction that proceeds as long as desired, generating a molecule of
100,000 nucleotides or more that contains up to approximately 1000
tandem copies or more of a sequence complementary to the
amplification target circle or open circle probe. This tandem
sequence DNA (TS-DNA) consists of, in the case of OCPs, alternating
target sequence and spacer sequence. Note that the spacer sequence
of the TS-DNA is the complement of the sequence between the left
target probe and the right target probe in the original open circle
probe.
[0154] During rolling circle replication one may additionally
include radioactive, or modified nucleotides such as
bromodeoxyuridine triphosphate, in order to label the DNA generated
in the reaction. Alternatively, one may include suitable precursors
that provide a binding moiety such as biotinylated nucleotides
(Langer et al. (1981)). Unmodified TS-DNA can be detected using any
nucleic acid detection technique.
[0155] As well as rolling circle replication, the amplification
operation can include additional nucleic acid replication or
amplification processes. For example, TS-DNA can itself be
replicated to form secondary TS-DNA. This process is referred to as
secondary DNA strand displacement. The combination of rolling
circle replication and secondary DNA strand displacement is
referred to as linear rolling circle amplification (LRCA). The
secondary TS-DNA can itself be replicated to form tertiary TS-DNA
in a process referred to as tertiary DNA strand displacement.
Secondary and tertiary DNA strand displacement can be performed
sequentially or simultaneously. When performed simultaneously, the
result is strand displacement cascade amplification. The
combination of rolling circle replication and strand displacement
cascade amplification is referred to as exponential rolling circle
amplification (ERCA). Secondary TS-DNA, tertiary TS-DNA, or both
can be amplified by transcription.
[0156] After RCA, a round of LM-RCA can be performed on the TS-DNA
produced in the first RCA. This new round of LM-RCA is performed
with a new open circle probe, referred to as a secondary open
circle probe, having target probe portions complementary to a
target sequence in the TS-DNA produced in the first round. When
such new rounds of LM-RCA are performed, the amplification is
referred to as nested LM-RCA. Nested LM-RCA can also be performed
on ligated OCPs or ATCs that have not been amplified. In this case,
LM-RCA can be carried out using either ATCs or target-dependent
ligated OCPs. This is especially useful for in situ detection. For
in situ detection, the first, unamplified OCP, which is
topologically locked to its target sequence, can be subjected to
nested LM-RCA. By not amplifying the first OCP, it can remain
hybridized to the target sequence while LM-RCA amplifies a
secondary OCP topologically locked to the first OCP. Nested LM-RCA
is described in U.S. Pat. No. 6,143,495.
[0157] C. Extension
[0158] The disclosed method uses hairpin probes and primers to
reduce or eliminate non-specific and other undesired nucleic acid
replication. This is accomplished by virtue of the probe and primer
design (as described above) and results in "inactivation" of the
probes and primer if they are not involved in legitimate hybrid.
Such inactivation refers to the reduced ability of the probe or
primer to hybridize to sequences other than their intended target
sequence. As used herein, inactivation of probes and primers does
not require complete loss of non-specific hybridization; reduction
in non-specific hybridization is sufficient.
[0159] The disclosed open circle probes can be inactivated in
several ways. For example, where the 3' end of an open circle probe
is involved in an intramolecular stem structure, the 3' end can be
extended in a replication reaction using the open circle probe
sequences as template (see FIG. 2B). The result is stabilization of
the intramolecular stem structure and a change in the 3' end
sequence. Stabilization of the stem structure results in a
reduction or elimination of the ability of the open circle probe to
prime nucleic acid synthesis because the 3' end is stably
hybridized to sequences in the open circle probe under the
conditions used for nucleic acid replication. Change in the
sequence of the 3' end can reduce of the ability of the open circle
probe to prime nucleic acid synthesis because the changed 3'
sequences may not be as closely related to sequences involved in
the amplification reaction or assay. Change in the sequence of the
3' end can reduce of the ability of the open circle probe to serve
as a template for rolling circle amplification. For example, even
if the open circle probe with extended 3' end were circularized,
the rolling circle replication primer could be prevented from
priming replication of such a circle if the primer complement
sequence on the open circle probe were interrupted by the added
sequences. This can be accomplished by, for example, designing the
open circle probe to have the primer complement sequence include
both 5' and 3' end sequences of the open circle probe.
[0160] D. Sequestration
[0161] The open circle probe can also be inactivated by formation
of the intramolecular stem structure during the amplification
reaction. As long as the end remains in the intramolecular stem
structure (that is, as long as it is sequestered in the stem
structure), it is not available for priming nucleic acid synthesis.
This form of inactivation is aided by design the intramolecular
stem structure, or selecting amplification conditions, such that
the intramolecular hybrid remains stable during rolling circle
amplification. Extension of the end as described above also results
in sequestration of the end in the intramolecular stem
structure.
[0162] Discrimination of rolling circle replication primer
hybridization also can be accomplished by hybridizing primer to
primer complement portions of OCPs or ATCs under conditions that
favor only exact sequence matches leaving other rolling circle
replication primers unhybridized. The unhybridized rolling circle
replication primers will retain or re-form the intramolecular
hybrid. Discrimination of DNA strand displacement primer
hybridization can be accomplished in a similar manner by
hybridizing primer to TS-DNA under conditions that favor only exact
sequence matches leaving other DNA strand displacement primers
unhybridized.
[0163] E. Loop Hybridization Disruption
[0164] One form of the disclosed open circle probes includes a loop
as part of the intramolecular stem structure. It is preferred that
the loop contain sequences complementary to the target sequence.
This allows the loop to nucleate hybridization of the open probe to
the target sequence. Preferred forms of the loop-containing probes
are characterized by a sequence discrimination capability that is
markedly better that the comparable linear probes due to the
competition between the structural interferences between folding
due to intramolecular stem formation and linear rigidity due to
hybridization of the probe sequence to the target (Tyagi and
Kramer, Nat Biotechnol 14(3):303-8 (1996); Bonnet et al., Proc Natl
Acad Sci USA 96(11):6171-6 (1999)). Preferred open circle probes of
this type will not hybridize to mismatched sequences under suitable
conditions because duplex hybridization of probe to target does not
effectively compete with intramolecular stem formation of the
structured probe. This makes the end(s) of the open circle probe
involved in an intramolecular stem structure unavailable for
ligation to circularize the probe and leave the 3' end available
for inactivating extension. The presence of target sequence causes
the correctly matched open circle probe to unfold, allowing the
ends to hybridize to the target sequence and be coupled (see FIG.
3). Where sequences in the loop nucleate hybridization of the open
circle probe to a target sequence, loop hybridization to a
non-target sequence is unlikely to lead to circularization of the
open circle probe. This is because it is unlikely that a non-target
sequence will include adjacent sequences to which both the loop and
open circle probe end can hybridize (see FIG. 4).
[0165] A hybridization nucleating loop can also be used in linear
primers used for nucleic acid replication and amplification. Such a
primer forms an intramolecular stem structure, including a loop.
Loop-containing primers of this type will not hybridize to
mismatched sequences under suitable conditions because duplex
hybridization of probe to target does not effectively compete with
intramolecular stem formation of the structured probe. This makes
the end of the primer involved in an intramolecular stem structure
unavailable for priming. The legitimate primer complement sequence
causes the correctly matched primer to unfold, allowing the end to
hybridize to the primer complement sequence and prime synthesis.
Where sequences in the loop nucleate hybridization of the primer,
loop hybridization to an illegitimate sequence is unlikely to lead
to priming. This is because it is unlikely that an illegitimate
sequence will include adjacent sequences to which both the loop and
the primer end can hybridize. Including proximity-sensitive labels
used in molecular beacon probes in such primers allows
hybridization and priming by the primers to be detected through
activation of the label upon disruption of the intramolecular stem
structure (Tyagi and Kramer, Nat Biotechnol 14(3):303-8 (1996);
Bonnet et al., Proc Natl Acad Sci USA 96(11):6171-6 (1999)).
[0166] G. Reporter Binding Agents With Target Sequences
[0167] A preferred form of the disclosed method uses reporter
binding agents having target sequences as the oligonucleotide
portion. The oligonucleotide portion of the reporter binding agent
serves as a target sequence. The affinity portion of the reporter
binding agent is a specific binding molecule specific for a target
molecule of interest, such as proteins or peptides. The reporter
binding agent is associated with the target molecule and detection
of this interaction is mediated by rolling circle amplification.
Unbound reporter binding agents can be removed by washing. Once the
reporter binding agent is associated with a target molecule, a open
circle probe is hybridized to the target sequence of the reporter
binding agent, ligated, and amplified. The resulting TS-DNA is
associated with the ligated open circle probe, thus associating the
TS-DNA to the site of the target molecule.
[0168] Reporter binding agents are preferably used with a
solid-state substrate and in combination with combinatorial
multicolor coding. For this purpose, samples to be tested are
incorporated into a solid-state sample, as described above. The
solid-state substrate is preferably a glass slide and the
solid-state sample preferably incorporates up to 256 individual
target or assay samples arranged in dots. Multiple solid-state
samples can be used to either test more individual samples, or to
increase the number of distinct target sequences to be detected. In
the later case, each solid-state sample has an identical set of
samples dots, and the assay will be carried out using a different
set of reporter binding agents and open circle probes, collectively
referred to as a probe set, for each solid-state sample. This
allows a large number of individuals and target sequences to be
assayed in a single assay. By using up to six different labels,
combinatorial multicolor coding allows up to 63 distinct targets to
be detected on a single solid-state sample. When using multiple
solid-state substrates and performing RCA with a different set of
reporter binding agents and open circle probes for each solid-state
substrate, the same labels can be used with each solid-state sample
(although differences between OCPs in each set may require the use
of different detection probes). For example, 10 replica slides,
each with 256 target sample dots, can be subjected to RCA using 10
different sets of reporter binding agents and open circle probes,
where each set is designed for combinatorial multicolor coding of
63 targets. This results in an assay for detection of 630 different
target molecules.
[0169] After rolling circle amplification, a cocktail of detection
probes is added, where the cocktail contains color combinations
that are specific for each OCP. The design and combination of such
detection probes for use in combinatorial multicolor coding is
described elsewhere herein. It is preferred that the OCPs be
designed with combinatorially coded detection tags to allow use of
a single set of singly labeled detection probes. It is also
preferred that collapsing detection probes be used.
[0170] H. Detection Of Amplification Products
[0171] Products of the amplification operation can be detected
using any nucleic acid detection technique. Many techniques are
known for detecting nucleic acids. Several preferred forms of
detection are described below. The nucleotide sequence of the
amplified sequences also can be determined using any suitable
technique.
[0172] 1. Primary Labeling
[0173] Primary labeling consists of incorporating labeled moieties,
such as fluorescent nucleotides, biotinylated nucleotides,
digoxygenin-containing nucleotides, or bromodeoxyuridine, during
rolling circle replication in RCA, or during transcription in RCT.
For example, one may incorporate cyanine dye UTP analogs (Yu et al.
(1994)) at a frequency of 4 analogs for every 100 nucleotides. A
preferred method for detecting nucleic acid amplified in situ is to
label the DNA during amplification with BrdUrd, followed by binding
of the incorporated BUDR with a biotinylated anti-BUDR antibody
(Zymed Labs, San Francisco, Calif.), followed by binding of the
biotin moieties with Streptavidin-Peroxidase (Life Sciences, Inc.),
and finally development of fluorescence with Fluorescein-tyramide
(DuPont de Nemours & Co., Medical Products Dept.).
[0174] 2. Secondary Labeling
[0175] Secondary labeling consists of using suitable molecular
probes, such as detection probes, to detect the amplified nucleic
acids. For example, an open circle may be designed to contain
several repeats of a known arbitrary sequence, referred to as
detection tags. A secondary hybridization step can be used to bind
detection probes to these detection tags (see FIG. 7 in U.S. Pat.
No. 6,143,495). The detection probes may be labeled as described
above with, for example, an enzyme, fluorescent moieties, or
radioactive isotopes. By using three detection tags per open circle
probe, and four fluorescent moieties per each detection probe, one
may obtain a total of twelve fluorescent signals for every open
circle probe repeat in the TS-DNA, yielding a total of 12,000
fluorescent moieties for every ligated open circle probe that is
amplified by RCA.
[0176] 3. Multiplexing and Hybridization Array Detection
[0177] RCA is easily multiplexed by using sets of different open
circle probes, each set carrying different target probe sequences
designed for binding to unique targets. Note that although the
target probe sequences designed for each target are different, the
primer complement portion may remain unchanged, and thus the primer
for rolling circle replication can remain the same for all targets.
Only those open circle probes that are able to find their targets
will give rise to TS-DNA. The TS-DNA molecules generated by RCA are
of high molecular weight and low complexity; the complexity being
the length of the open circle probe. There are two alternatives for
capturing a given TS-DNA to a fixed position in a solid-state
detector. One is to include within the spacer region of the open
circle probes a unique address tag sequence for each unique open
circle probe. TS-DNA generated from a given open circle probe will
then contain sequences corresponding to a specific address tag
sequence. A second and preferred alternative is to use the target
sequence present on the TS-DNA as the address tag.
[0178] 4. Combinatorial Multicolor Coding
[0179] A preferred form of multiplex detection involves the use of
a combination of labels that either fluoresce at different
wavelengths or are colored differently. One of the advantages of
fluorescence for the detection of hybridization probes is that
several targets can be visualized simultaneously in the same
sample. Using a combinatorial strategy, many more targets can be
discriminated than the number of spectrally resolvable
fluorophores. Combinatorial labeling provides the simplest way to
label probes in a multiplex fashion since a probe fluor is either
completely absent (-) or present in unit amounts (+); image
analysis is thus more amenable to automation, and a number of
experimental artifacts, such as differential photobleaching of the
fluors and the effects of changing excitation source power
spectrum, are avoided.
[0180] The combinations of labels establish a code for identifying
different detection probes and, by extension, different target
molecules to which those detection probes are associated with. This
labeling scheme is referred to as Combinatorial Multicolor Coding
(CMC). Such coding is described by Speicher et al., Nature Genetics
12:368-375 (1996). Use of CMC in connection with rolling circle
amplification is described in U.S. Pat. No. 6,143,495. Any number
of labels, which when combined can be separately detected, can be
used for combinatorial multicolor coding. It is preferred that 2,
3, 4, 5, or 6 labels be used in combination. It is most preferred
that 6 labels be used. The number of labels used establishes the
number of unique label combinations that can be formed according to
the formula 2.sup.N-1, where N is the number of labels. According
to this formula, 2 labels forms three label combinations, 3 labels
forms seven label combinations, 4 labels forms 15 label
combinations, 5 labels form 31 label combinations, and 6 labels
forms 63 label combinations.
[0181] For combinatorial multicolor coding, a group of different
detection probes are used as a set. Each type of detection probe in
the set is labeled with a specific and unique combination of
fluorescent labels. For those detection probes assigned multiple
labels, the labeling can be accomplished by labeling each detection
probe molecule with all of the required labels. Alternatively,
pools of detection probes of a given type can each be labeled with
one of the required labels. By combining the pools, the detection
probes will, as a group, contain the combination of labels required
for that type of detection probe. Where each detection probe is
labeled with a single label, label combinations can also be
generated by using OCPs or ATCs with coded combinations of
detection tags complementary to the different detection probes. In
this scheme, the OCPs or ATCs will contain a combination of
detection tags representing the combination of labels required for
a specific label code. Further illustrations are described in U.S.
Pat. No. 6,143,495.
[0182] As described below, rolling circle amplification can be
engineered to produce TS-DNA of different lengths in an assay
involving multiple ligated OCPs or ATCs. The resulting TS-DNA of
different length can be distinguished simply on the basis of the
size of the detection signal they generate. Thus, the same set of
detection probes could be used to distinguish two different sets of
generated TS-DNA. In this scheme, two different TS-DNAs, each of a
different size but assigned the same color code, would be
distinguished by the size of the signal produced by the hybridized
detection probes. In this way, a total of 126 different targets can
be distinguished on a single solid-state sample using a code with
63 combinations, since the signals will come in two flavors, low
amplitude and high amplitude. Thus one could, for example, use the
low amplitude signal set of 63 probes for detection of an oncogene
mutations, and the high amplitude signal set of 63 probes for the
detection of a tumor suppressor p53 mutations.
[0183] Speicher et al. describes a set of fluors and corresponding
optical filters spaced across the spectral interval 350-770 nm that
give a high degree of discrimination between all possible fluor
pairs. This fluor set, which is preferred for combinatorial
multicolor coding, consists of 4'-6-diamidino-2-phenylinodole
(DAPI), fluorescein (FITC), and the cyanine dyes Cy3, Cy3.5, Cy5,
Cy5.5 and Cy7. Any subset of this preferred set can also be used
where fewer combinations are required. The absorption and emission
maxima, respectively, for these fluors are: DAPI (350 nm; 456 nm),
FITC (490 nm; 520 nm), Cy3 (554 nm; 568 nm), Cy3.5 (581 nm; 588
nm), Cy5 (652 nm; 672 nm), Cy5.5 (682 nm; 703 nm) and Cy7 (755 nm;
778 nm). The excitation and emission spectra, extinction
coefficients and quantum yield of these fluors are described by
Ernst et al., Cytometry 10:3-10 (1989), Mujumdar et al., Cytometry
10:11-19 (1989), Yu, Nucleic Acids Res. 22:3226-3232 (1994), and
Waggoner, Meth. Enzymology 246:362-373 (1995). These fluors can all
be excited with a 75W Xenon arc.
[0184] To attain selectivity, filters with bandwidths in the range
of 5 to 16 nm are preferred. To increase signal discrimination, the
fluors can be both excited and detected at wavelengths far from
their spectral maxima. Emission bandwidths can be made as wide as
possible. For low-noise detectors, such as cooled CCD cameras,
restricting the excitation bandwidth has little effect on
attainable signal to noise ratios. A list of preferred filters for
use with the preferred fluor set is listed in Table 1 of Speicher
et al. It is important to prevent infra-red light emitted by the
arc lamp from reaching the detector; CCD chips are extremely
sensitive in this region. For this purpose, appropriate IR blocking
filters can be inserted in the image path immediately in front of
the CCD window to minimize loss of image quality. Image analysis
software can then be used to count and analyze the spectral
signatures of fluorescent dots.
[0185] Discrimination of individual signals in combinatorial
multicolor coding can be enhanced by collapsing TS-DNA generated
during amplification. As described elsewhere herein, this is
preferably accomplished using collapsing detection probes,
biotin-antibody conjugates, or a combination of both. A collapsed
TS-DNA can occupy a space of no more than 0.3 microns in diameter.
Based on this, it is expected that up to a million discrete signals
can be detected in a 2.5 mm sample dot. Such discrimination also
results in a large dynamic range for quantitative signal detection.
Thus, the relative numbers of different types of signals (such as
multicolor codes) can be determined over a wide range. This is
expected to allow determination of, for example, the relative
amount of different target molecules, such as proteins, in a
sample. Such comparative detections would be useful in, for
example, proteomics analyses of cell and tissue samples. This would
also allow determination of whether a particular target sequence is
homozygous or heterozygous in a genomic DNA sample, whether a
target sequence was inherited or represents a somatic mutation, and
the genetic heterogeneity of a genomic DNA sample, such as a tumor
sample.
[0186] 5. Detecting Multiple Target Sequences
[0187] Multiplex RCA assays are particularly useful for detecting
multiple proteins. A single LM-RCA assay can be used to detect the
presence of one or more members of a group of any number of target
sequences. By associating different target sequences with different
proteins (using reporter binding agents specific for the proteins
of interest), each different protein can be detected by
differential detection of the various target sequences. This can be
accomplished, for example, by designing an open circle probe (and
associated gap oligonucleotides, if desired) for each target
sequence in the group, where the target probe portions and the
sequence of the detection tag portions of each open circle probe
are different but the sequence of the primer portions of all the
open circle probes are the same. All of the open circle probes are
placed in the same OCP-target sample mixture, and the same primer
is used to amplify. Different detection probes are used to detect
the various TS-DNAs (each having specific detection tag sequences).
For each target sequence present in the assay (those associated
with proteins present in the target sample, for example), the OCP
for that target will be ligated into a circle and the circle will
be amplified to form TS-DNA. Since the detection tags on TS-DNA
resulting from amplification of the OCPs are the different, TS-DNA
resulting from ligation each OCP can be detected individually in
that assay.
[0188] 6. Detecting Groups of Target Sequences
[0189] Multiplex RCA assays are particularly useful for detecting
any of a set of target sequences in a defined group. For example,
the disclosed method can be used to detect mutations in genes where
numerous distinct mutations are associated with certain diseases or
where mutations in multiple genes are involved. For example,
although the gene responsible for Huntington's chorea has been
identified, a wide range of mutations in different parts of the
gene occur among affected individuals. The result is that no single
test has been devised to detect whether an individual has one or
more of the many Huntington's mutations. A single LM-RCA assay can
be used to detect the presence of one or more members of a group of
any number of target sequences. This can be accomplished, for
example, by designing an open circle probe (and associated gap
oligonucleotides, if desired) for each target sequence in the
group, where the target probe portions of each open circle probe
are different but the sequence of the primer portions and the
sequence of the detection tag portions of all the open circle
probes are the same. All of the open circle probes are placed in
the same OCP-target sample mixture, and the same primer and
detection probe are used to amplify and detect TS-DNA. If any of
the target sequences are present in the target sample, the OCP for
that target will be ligated into a circle and the circle will be
amplified to form TS-DNA. Since the detection tags on TS-DNA
resulting from amplification of any of the OCPs are the same,
TS-DNA resulting from ligation of any of the OCPs will be detected
in that assay. Detection indicates that at least one member of the
target sequence group is present in the target sample. This allows
detection of a trait associated with multiple target sequences in a
single tube or well.
[0190] If a positive result is found, the specific target sequence
involved can be identified by using a multiplex assay. This can be
facilitated by including an additional, different detection tag in
each of the OCPs of the group. In this way, TS-DNA generated from
each different OCP, representing each different target sequence,
can be individually detected. It is convenient that such multiple
assays need be performed only when an initial positive result is
found.
[0191] The above scheme can also be used with arbitrarily chosen
groups of target sequences in order to screen for a large number of
target sequences without having to perform an equally large number
of assays. Initial assays can be performed as described above, each
using a different group of OCPs designed to hybridize to a
different group of target sequences. Additional assays to determine
which target sequence is present can then be performed on only
those groups that produce TS-DNA. Such group assays can be further
nested if desired.
[0192] 7. In Situ Detection Using RCA
[0193] In situ detection of target sequences is a powerful
application of the disclosed method. For example, open circle
probes can be ligated on targets immobilized on a substrate, and
incubated in situ with fluorescent precursors during rolling circle
replication. The circle will remain topologically trapped on the
chromosome unless the DNA is nicked (Nilsson et al. (1994)). The
resulting TS-DNA will then be associated with the location of the
target sequence.
[0194] A preferred method of in situ detection uses reporter
binding agents having target sequences as the oligonucleotide
portion. In this form of the method, reporter binding agents having
target sequences as the oligonucleotide portion are associated with
target molecules (such as proteins) that are immobilized or
otherwise attached to a substrate. Once the reporter binding agent
is associated with a target molecule, an open circle probe is
hybridized to the target sequence of the reporter binding agent and
circularized. The circularized open circle probe is then amplified.
The resulting TS-DNA is associated with the site of the target
molecule via the open circle probe and reporter binding agent.
[0195] Localization of the TS-DNA for in situ detection can also be
enhanced by collapsing the TS-DNA using collapsing detection
probes, biotin-antibody conjugates, or both, as described elsewhere
herein. Multiplexed in situ detection can be carried out as
follows: Rolling circle replication is carried out using unlabeled
nucleotides. The different TS-DNAs are then detected using standard
multi-color FISH with detection probes specific for each unique
target sequence or each unique detection tag in the TS-DNA.
Alternatively, and preferably, combinatorial multicolor coding, as
described above, can be used for multiplex in situ detection.
[0196] Another method of in situ detection uses reporter binding
agents having rolling circle replication primers as the
oligonucleotide portion (this is referred to as Reporter Binding
Agent Unimolecular Rolling Amplification (RBAURA) in U.S. Pat. No.
6,143,495). In RBAURA, a reporter binding agent is used where the
oligonucleotide portion serves as a rolling circle replication
primer. Once the reporter binding agent is associated with a target
molecule, an amplification target circle is hybridized to the
rolling circle replication primer sequence of the reporter binding
agent followed by amplification of the ATC by RCA. The resulting
TS-DNA has the rolling circle replication primer sequence of the
reporter binding agent at one end, thus anchoring the TS-DNA to the
site of the target molecule. Peptide Nucleic Acid Probe
Unimolecular Rolling Amplification (PNAPURA) and Locked Antibody
Unimolecular Rolling Amplification (LAURA), described in U.S. Pat.
No. 6,143,495, are preferred forms of RBAURA.
[0197] 8. Enzyme-Linked Detection
[0198] Amplified nucleic acid labeled by incorporation of labeled
nucleotides can be detected with established enzyme-linked
detection systems. For example, amplified nucleic acid labeled by
incorporation of biotin-16-UTP (Boehringher Mannheim) can be
detected as follows. The nucleic acid is immobilized on a solid
glass surface by hybridization with a complementary DNA
oligonucleotide (address probe) complementary to the target
sequence (or its complement) present in the amplified nucleic acid.
After hybridization, the glass slide is washed and contacted with
alkaline phosphatase-streptavidin conjugate (Tropix, Inc., Bedford,
Mass.). This enzyme-streptavidin conjugate binds to the biotin
moieties on the amplified nucleic acid. The slide is again washed
to remove excess enzyme conjugate and the chemiluminescent
substrate CSPD (Tropix, Inc.) is added and covered with a glass
cover slip. The slide can then be imaged in a Biorad
Fluorimager.
[0199] 9. Collapse of Nucleic Acids
[0200] Tandem sequence DNA or TS-RNA, which are produced as
extended nucleic acid molecules, can be collapsed into a compact
structure. It is preferred that the nucleic acid to be collapsed is
immobilized on a substrate. A preferred means of collapsing nucleic
acids is by hybridizing one or more collapsing probes with the
nucleic acid to be collapsed. Collapsing probes are
oligonucleotides having a plurality of portions each complementary
to sequences in the nucleic acid to be collapsed. These portions
are referred to as complementary portions of the collapsing probe,
where each complementary portion is complementary to a sequence in
the nucleic acid to be collapsed. The sequences in the nucleic acid
to be collapsed are referred to as collapsing target sequences. The
complementary portion of a collapsing probe can be any length that
supports specific and stable hybridization between the collapsing
probe and the collapsing target sequence. For this purpose, a
length of 10 to 35 nucleotides is preferred, with a complementary
portion of a collapsing probe 16 to 20 nucleotides long being most
preferred. It is preferred that at least two of the complementary
portions of a collapsing probe be complementary to collapsing
target sequences which are separated on the nucleic acid to be
collapsed or to collapsing target sequences present in separate
nucleic acid molecules. This allows each detection probe to
hybridize to at least two separate collapsing target sequences in
the nucleic acid sample. In this way, the collapsing probe forms a
bridge between different parts of the nucleic acid to be collapsed.
The combined action of numerous collapsing probes hybridizing to
the nucleic acid will be to form a collapsed network of
cross-linked nucleic acid. Collapsed nucleic acid occupies a much
smaller volume than free, extended nucleic acid, and includes
whatever detection probe or detection label hybridized to the
nucleic acid. This result is a compact and discrete nucleic acid
structure which can be more easily detected than extended nucleic
acid. Collapsing nucleic acids is useful both for in situ
hybridization applications and for multiplex detection because it
allows detectable signals to be spatially separate even when
closely packed. Collapsing nucleic acids is especially preferred
for use with combinatorial multicolor coding. Collapsing probes can
also contain any of the detection labels described above. TS-DNA
collapse can also be accomplished through the use of ligand/ligand
binding pairs (such as biotin and avidin) or hapten/antibody pairs.
Nucleic acid collapse is further described in U.S. Pat. No.
6,143,495.
[0201] I. DNA Strand Displacement
[0202] DNA strand displacement is one way to amplify TS-DNA.
Secondary DNA strand displacement is accomplished by hybridizing
secondary DNA strand displacement primers to TS-DNA and allowing a
DNA polymerase to synthesize DNA from these primed sites (see FIG.
11 in U.S. Pat. No. 6,143,495). Because a complement of the
secondary DNA strand displacement primer occurs in each repeat of
the TS-DNA, secondary DNA strand displacement can result in a high
level of amplification. The product of secondary DNA strand
displacement is referred to as secondary tandem sequence DNA or
TS-DNA-2. Secondary DNA strand displacement can be accomplished by
performing RCA to produce TS-DNA, mixing secondary DNA strand
displacement primer with the TS-DNA, and incubating under
conditions promoting replication of the tandem sequence DNA. The
disclosed hairpin open circle probes are especially useful for DNA
strand displacement because inactivated hairpin open circle probes
will not compete with secondary DNA strand displacement primers for
hybridization to TS-DNA. The DNA strand displacement primers are
preferably hairpin DNA strand displacement primers.
[0203] Secondary DNA strand displacement can also be carried out
simultaneously with rolling circle replication. This is
accomplished by mixing secondary DNA strand displacement primer
with the reaction prior to rolling circle replication. As a
secondary DNA strand displacement primer is elongated, the DNA
polymerase will run into the 5' end of the next hybridized
secondary DNA strand displacement molecule and will displace its 5'
end. In this fashion a tandem queue of elongating DNA polymerases
is formed on the TS-DNA template. As long as the rolling circle
reaction continues, new secondary DNA strand displacement primers
and new DNA polymerases are added to TS-DNA at the growing end of
the rolling circle. The generation of TS-DNA-2 and its release into
solution by strand displacement is shown diagrammatically in FIG.
11 in U.S. Pat. No.6,143,495. For simultaneous rolling circle
replication and secondary DNA strand displacement, it is preferred
that the rolling circle DNA polymerase be used for both
replications. This allows optimum conditions to be used and results
in displacement of other strands being synthesized downstream.
Secondary DNA strand displacement can follow any DNA replication
operation, such as RCA, LM-RCA or nested LM-RCA.
[0204] Generally, secondary DNA strand displacement can be
performed by, simultaneous with or following RCA, mixing a
secondary DNA strand displacement primer with the reaction mixture
and incubating under conditions that promote both hybridization
between the tandem sequence DNA and the secondary DNA strand
displacement primer, and replication of the tandem sequence DNA,
where replication of the tandem sequence DNA results in the
formation of secondary tandem sequence DNA.
[0205] When secondary DNA strand displacement is carried out in the
presence of a tertiary DNA strand displacement primer, an
exponential amplification of TS-DNA sequences takes place. This
special and preferred mode of DNA strand displacement is referred
to as strand displacement cascade amplification (SDCA). In SDCA, a
secondary DNA strand displacement primer primes replication of
TS-DNA to form TS-DNA-2, as described above. The tertiary DNA
strand displacement primer strand can then hybridize to, and prime
replication of, TS-DNA-2 to form TS-DNA-3. Strand displacement of
TS-DNA-3 by the adjacent, growing TS-DNA-3 strands makes TS-DNA-3
available for hybridization with secondary DNA strand displacement
primer. This results in another round of replication resulting in
TS-DNA-4 (which is equivalent to TS-DNA-2). TS-DNA-4, in turn,
becomes a template for DNA replication primed by tertiary DNA
strand displacement primer. The cascade continues this manner until
the reaction stops or reagents become limiting. This reaction
amplifies DNA at an almost exponential rate. In a preferred mode of
SDCA, the rolling circle replication primer serves as the tertiary
DNA strand displacement primer, thus eliminating the need for a
separate primer.
[0206] For this mode, the rolling circle replication primer should
be used at a concentration sufficiently high to obtain rapid
priming on the growing TS-DNA-2 strands. To optimize the efficiency
of SDCA, it is preferred that a sufficient concentration of
secondary DNA strand displacement primer and tertiary DNA strand
displacement primer be used to obtain sufficiently rapid priming of
the growing TS-DNA strand to outcompete TS-DNA for binding to its
complementary TS-DNA. Optimization of primer concentrations are
described in U.S. Pat. No. 6,143,495 and can be aided by analysis
of hybridization kinetics (Young and Anderson, "Quantitative
analysis of solution hybridization" in Nucleic Acid Hybridization:
A Practical Approach (IRL Press, 1985) pages 47-71).
[0207] Generally, strand displacement cascade amplification can be
performed by, simultaneous with, or following, RCA, mixing a
secondary DNA strand displacement primer and a tertiary DNA strand
displacement primer with the reaction mixture and incubating under
conditions that promote hybridization between the tandem sequence
DNA and the secondary DNA strand displacement primer, replication
of the tandem sequence DNA--where replication of the tandem
sequence DNA results in the formation of secondary tandem sequence
DNA--hybridization between the secondary tandem sequence DNA and
the tertiary DNA strand displacement primer, and replication of
secondary tandem sequence DNA--where replication of the secondary
tandem sequence DNA results in formation of tertiary tandem
sequence DNA (TS-DNA-3).
[0208] Secondary and tertiary DNA strand displacement can also be
carried out sequentially. Following a first round of secondary DNA
strand displacement, a tertiary DNA strand displacement primer can
be mixed with the secondary tandem sequence DNA and incubated under
conditions that promote hybridization between the secondary tandem
sequence DNA and the tertiary DNA strand displacement primer, and
replication of secondary tandem sequence DNA, where replication of
the secondary tandem sequence DNA results in formation of tertiary
tandem sequence DNA (TS-DNA-3). This round of strand displacement
replication can be referred to as tertiary DNA strand displacement.
However, all rounds of strand displacement replication following
rolling circle replication can also be referred to collectively as
DNA strand displacement or secondary DNA strand displacement.
[0209] A modified form of secondary DNA strand displacement results
in amplification of TS-DNA and is referred to as opposite strand
amplification (OSA). OSA is the same as secondary DNA strand
displacement except that a special form of rolling circle
replication primer is used that prevents it from hybridizing to
TS-DNA-2. Opposite strand amplification is described in U.S. Pat.
No. 6,143,495.
[0210] The DNA generated by DNA strand displacement can be labeled
and/or detected using the same labels, labeling methods, and
detection methods described for use with TS-DNA. Most of these
labels and methods are adaptable for use with nucleic acids in
general. A preferred method of labeling the DNA is by incorporation
of labeled nucleotides during synthesis.
[0211] J. Transcription Following RCA
[0212] Once TS-DNA is generated using RCA, further amplification
can be accomplished by transcribing the TS-DNA from promoters
embedded in the TS-DNA. This combined process, referred to as
rolling circle replication with transcription (RCT), or ligation
mediated rolling circle replication with transcription (LM-RCT),
requires that the OCP or ATC from which the TS-DNA is made have a
promoter portion in its spacer region. The promoter portion is then
amplified along with the rest of the OCP or ATC resulting in a
promoter embedded in each tandem repeat of the TS-DNA. Because
transcription, like rolling circle amplification, is a process that
can go on continuously (with re-initiation), multiple transcripts
can be produced from each of the multiple promoters present in the
TS-DNA. RCT effectively adds another level of amplification of
ligated OCP sequences.
[0213] Generally, RCT can be accomplished by performing RCA to
produce TS-DNA, and then mixing RNA polymerase with the reaction
mixture and incubating under conditions promoting transcription of
the tandem sequence DNA. The OCP or ATC must include the sequence
of a promoter for the RNA polymerase (a promoter portion) in its
spacer region for RCT to work. The transcription step in RCT
generally can be performed using established conditions for in
vitro transcription of the particular RNA polymerase used.
Alternatively, transcription can be carried out simultaneously with
rolling circle replication. This is accomplished by mixing RNA
polymerase with the reaction mixture prior to rolling circle
replication. Transcription can follow any DNA replication
operation, such as RCA, LM-RCA, nested LM-RCA, DNA strand
displacement, or strand displacement cascade amplification.
[0214] The transcripts generated in RCT can be labeled and/or
detected using the same labels, labeling methods, and detection
methods described for use with TS-DNA. Most of these labels and
methods are adaptable for use with nucleic acids in general. A
preferred method of labeling RCT transcripts is by direct labeling
of the transcripts by incorporation of labeled nucleotides, most
preferably biotinylated nucleotides, during transcription.
[0215] K. Gap-Filling Ligation
[0216] The gap space formed by an OCP hybridized to a target
sequence is normally occupied by one or more gap oligonucleotides
as described above. Such a gap space may also be filled in by a
gap-filling DNA polymerase during the ligation operation. As an
alternative, the gap space can be partially bridged by one or more
gap oligonucleotides, with the remainder of the gap filled using
DNA polymerase. This modified ligation operation is referred to
herein as gap-filling ligation and is a preferred form of the
ligation operation. The principles and procedure for gap-filling
ligation are generally analogous to the filling and ligation
performed in gap LCR (Wiedmann et al., PCR Methods and Applications
(Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Laboratory, N.Y., 1994) pages S51 -S64; Abravaya et al., Nucleic
Acids Res., 23(4):675-682 (1995); European Patent Application
EP0439182 (1991)). In the case of LM-RCA, the gap-filling ligation
operation is substituted for the normal ligation operation.
Gap-filling ligation provides a means for discriminating between
closely related target sequences. Gap-filling ligation can be
accomplished by using a different DNA polymerase, referred to
herein as a gap-filling DNA polymerase. Suitable gap-filling DNA
polymerases are described above. Alternatively, DNA polymerases in
general can be used to fill the gap when a stop base is used. The
use of stop bases in the gap-filling operation of LCR is described
in European Patent Application EP0439182. The principles of the
design of gaps and the ends of flanking probes to be joined, as
described in EP0439182, is generally applicable to the design of
the gap spaces and the ends of target probe portions described
herein. Gap-filling ligation is further described in U.S. Pat. No.
6,143,495.
[0217] L. Reporter Binding Agent Unimolecular Rolling
Amplification
[0218] Reporter Binding Agent Unimolecular Rolling Amplification
(RBAURA) is a form of RCA where a reporter binding agent provides
the rolling circle replication primer for amplification of an
amplification target circle. In RBAURA, the oligonucleotide portion
of the reporter binding agent serves as a rolling circle
replication primer. The rolling circle replication primer should be
a hairpin rolling circle replication primer. RBAURA allows RCA to
produce an amplified signal (that is, TS-DNA) based on association
of the reporter binding agent to a target molecule. The specific
primer sequence that is a part of the reporter binding agent
provides the link between the specific interaction of the reporter
binding agent to a target molecule (via the affinity portion of the
reporter binding agent) and RCA. In RBAURA, once the reporter
binding agent is associated with a target molecule, an
amplification target circle is hybridized to the rolling circle
replication primer sequence of the reporter binding agent, followed
by amplification of the ATC by RCA. The resulting TS-DNA
incorporates the rolling circle replication primer sequence of the
reporter binding agent at one end, thus anchoring the TS-DNA to the
site of the target molecule. RBAURA is a preferred RCA method for
iii situ detections. For this purpose, it is preferred that the
TS-DNA is collapsed using collapsing detection probes,
biotin-antibody conjugates, or both, as described above. RBAURA can
be performed using any target molecule. Preferred target molecules
are nucleic acids, including amplified nucleic acids such as TS-DNA
and amplification target circles, antigens and ligands. Examples of
the use of such target molecules are described in U.S. Pat. No.
6,143,495. Peptide Nucleic Acid Probe Unimolecular Rolling
Amplification (PNAPURA) and Locked Antibody Unimolecular Rolling
Amplification (LAURA), described in U.S. Pat. No. 6,143,495, are
preferred forms of RBAURA.
[0219] M. Discrimination Between Closely Related Target
Sequences
[0220] Open circle probes, gap oligonucleotides, and gap spaces can
be designed to discriminate closely related target sequences, such
as genetic alleles. Where closely related target sequences differ
at a single nucleotide, it is preferred that open circle probes be
designed with the complement of this nucleotide occurring at one
end of the open circle probe, or at one of the ends of the gap
oligonucleotide(s). Where gap-filling ligation is used, it is
preferred that the distinguishing nucleotide appear opposite the
gap space. This allows incorporation of alternative (that is,
allelic) sequence into the ligated OCP without the need for
alternative gap oligonucleotides. Where gap-filling ligation is
used with a gap oligonucleotide(s) that partially fills the gap, it
is preferred that the distinguishing nucleotide appear opposite the
portion of gap space not filled by a gap oligonucleotide. Ligation
of gap oligonucleotides with a mismatch at either terminus is
extremely unlikely because of the combined effects of hybrid
instability and enzyme discrimination. When the TS-DNA is
generated, it will carry a copy of the gap oligonucleotide sequence
that led to a correct ligation. Gap oligonucleotides may give even
greater discrimination between related target sequences in certain
circumstances, such as those involving wobble base pairing of
alleles. Features of open circle probes and gap oligonucleotides
that increase the target-dependency of the ligation operation are
generally analogous to such features developed for use with the
ligation chain reaction. These features can be incorporated into
open circle probes and gap oligonucleotides for use in LM-RCA. In
particular, European Patent Application EP0439182 describes several
features for enhancing target-dependency in LCR that can be adapted
for use in LM-RCA. The use of stop bases in the gap space, as
described in European Patent Application EP0439182, is a preferred
mode of enhancing the target discrimination of a gap-filling
ligation operation.
[0221] A preferred form of target sequence discrimination can be
accomplished by employing two types of open circle probes. In one
embodiment, a single gap oligonucleotide is used which is the same
for both target sequences, that is, the gap oligonucleotide is
complementary to both target sequences. In a preferred embodiment,
a gap-filling ligation operation can be used (Example 3 in U.S.
Pat. No. 6,143,495). Target sequence discrimination would occur by
virtue of mutually exclusive ligation events, or extension-ligation
events, for which only one of the two open-circle probes is
competent. Preferably, the discriminator nucleotide would be
located at the penultimate nucleotide from the 3' end of each of
the open circle probes. The two open circle probes would also
contain two different detection tags designed to bind alternative
detection probes and/or address probes. Each of the two detection
probes would have a different detection label. Both open circle
probes would have the same primer complement portion. Thus, both
ligated open circle probes can be amplified using a single primer.
Upon array hybridization, each detection probe would produce a
unique signal, for example, two alternative fluorescence colors,
corresponding to the alternative target sequences.
[0222] N. Size Classes of Tandem Sequence DNA
[0223] Rolling circle amplification can be engineered to produce
TS-DNA of different lengths in an assay involving multiple ligated
OCPs or ATCs. This can be useful for extending the number of
different targets that can be detected in a single assay. TS-DNA of
different lengths can be produced in several ways. In one
embodiment, the base composition of the spacer region of different
classes of OCP or ATC can be designed to be rich in a particular
nucleotide. Then a small amount of the dideoxy nucleotide
complementary to the enriched nucleotide can be included in the
rolling circle amplification reaction. After some amplification,
the dideoxy nucleotides will terminate extension of the TS-DNA
product of the class of OCP or ATC enriched for the complementary
nucleotide. Other OCPs or ATCs will be less likely to be
terminated, since they are not enriched for the complementary
nucleotide, and will produce longer TS-DNA products, on
average.
[0224] In another embodiment, two different classes of OCP or ATC
can be designed with different primer complement portions. These
different primer complement portions are designed to be
complementary to a different rolling circle replication primer.
Then the two different rolling circle replication primers are used
together in a single rolling circle amplification reaction, but at
significantly different concentrations. The primer at high
concentration immediately primes rolling circle replication due to
favorable kinetics, while the primer at lower concentration is
delayed in priming due to unfavorable kinetics. Thus, the TS-DNA
product of the class of OCP or ATC designed for the primer at high
concentration will be longer than the TS-DNA product of the class
of OCP or ATC designed for the primer at lower concentration since
it will have been replicated for a longer period of time. These and
other techniques for producing size classes of TS-DNA are described
in U.S. Pat. No. 6,143,495.
EXAMPLES
Example 1:
[0225] Primer Extension Assay
[0226] This example demonstrates that a hairpin open circle probe
with a 5' overhanging end can be extended from the 3' end. Such
extension would lead to an inactive open circle probe. Open circle
probe 1822ocT was used as a model for the new design of 3' hairpin
open circle probes. The new design was compared to the conventional
design.
1 Conventional OCP design sequence: 5'-phosphate-GAAGAACTGG-
ACAGATTTACTACGTATGTTGACTGGT
CACACGTCGTTCTAGTACGCTTCTACTCCCTCTTGAGAT- GTTCTGCTTTG TT 3' New
(hairpin) OCP design sequence:
5'-phosphate-GAAGAACTGGACAGATTTACTACGTATGTTGACTGGT
CACACGTCGTTCTAGTAACAAAGCACTCCCTCTTGAGATGTTCTGCTTTG TT 3'
[0227] .gamma..sup.32P ATP exchange reaction end labeling: M13
bacteriophage forward primer and OCPs were end labeled with
radioactive .sup.32P in an exchange reaction as follows:
[0228] 30 minutes exchange reactions were carried out in 20 .mu.l
volume containing 10 .mu.l of M13 bacteriophage forward primer or
open circle probe DNA (1pM/.mu.l), 4 .mu.l 5X exchange buffer
(Gibco BRL, cat # 10456-010) (250 mM imidazole-HCl (pH 6.4), 60 mM
MgCh.sub.2, 5 mM 2-mercaptoethanol, and 0.35 mM ADP), 0.5 .mu.l T4
polynucleotide kinase (NEB) (10 .mu.l), 2.5 .mu.l dH2O, and 3 .mu.l
10 mm .gamma..sup.32P ATP (10 .mu.Ci/.mu.l). DNA was purified and
eluted into 100 .mu.l of EB buffer (10 mM Tris-HCl, pH 8.5) using
QIAquick nucleotide removal kit (QIAGEN).
[0229] Annealing of .gamma..sup.32P ATP labeled forward primer to
M13 DNA: Annealing reaction was carried out by mixing: 3.3 .mu.l
forward primer, 7.6 .mu.l M13 mp19 ssDNA (0.25 .mu.g/.mu.l), 1
.mu.l Tris-HCl (pH 7.54), and 18.1 .mu.l dH.sub.2O. Heated to
95.degree. C. for 2 minutes, and cooled to room temperature.
[0230] M13 ladder preparation: 4 .mu.l of the above reaction was
added to 1 .mu.l 0.1M DTT, 7.6 .mu.l 50X sequenase buffer (260 mM
Tris-HCl, pH 9.5, 65 mM MgCl.sub.2), 7.6 .mu.l dH.sub.2O, and 0.4
.mu.l sequenase (USB) (4 units/.mu.l). The mix was divided into 4
equal portions and 2.5 .mu.l of ddG, ddA, ddT, or ddC was added
into each portion. All four portions were then incubated at
37.degree. C. for 5 minutes.
[0231] Primer extension reactions: 30 .mu.l extension reactions
were performed as follows: 0.1 .mu.l of labeled open circle probe
was added to ligation and ERCA reaction mix containing: 1 .mu.l
Ampligase buffer (20 mM Tris-HCl, pH 8.3) (Epicentre Technologies),
3 .mu.l 10.times. modified ThermoPol reaction buffer (200 mM
Tris-HCl, pH 8.8, 100 mM KCl, 100 mM (NH.sub.4).sub.2SO.sub.4 and
1% Triton X-100), 3 .mu.l 50% TMA oxalate, 20.7 .mu.l dH.sub.2O,
1.2 .mu.l 10 mM dNTP mix (dATP, dCTP, dGTP, and TTP), and 1 .mu.l
Bst polymerase (8 units/.mu.l) (New England Biolabs, Mass.), the
mix was incubated at 60.degree. C.
[0232] 3 .mu.l Aliquots were pipetted out between 15 seconds and 2
hr time points and added into3 .mu.l 2.times. stop buffer (95%
formamide, 20 mM EDTA, 0.05% bromophenol blue, and 0.05% xylene
cyanol FF). Samples were then boiled for 5 minutes and
electrophoresed on a 8% denaturing polyacrylamide gel. For each
open circle probe, aliquot number one was taken prior to adding the
polymerase enzyme, which represents the unextended open circle
probe.
[0233] Results: A hairpin forms at the 3' end of the open circle
probe that allowed the ERCA DNA polymerase to extend 54 bases from
the self-annealed 3' end of the open circle probe. Full extension
should have converted the hairpin open circle probe to an inert
double-stranded form. This reaction, called the "suicide pathway",
inactivates the open circle probe. The reaction was completed
within 15 sec of the start of primer extension reaction.
Example 2
[0234] VCAM SNiPer Assay
[0235] This example describes single nucleotide polymorphism (SNP)
detection on genomic DNA, using exponential rolling circle
amplification (ERCA). Specifically, Exponential Rolling Circle
Amplification is used for allele discrimination on genomic DNA on
an ABI Prism 7700 Sequence Detection System using generic P1
Amplifluors as detection probes.
[0236] Oligonucleotide sequences:
2 VCAMinA sequence: 5'-phosphate-AAATTGATTCAGGAAATACTAGCTTA-
TAAAGACTCGT CATGTCTCAGCTCTAGTTTCTGATCCCATGACTTCACCTACCAAATATCT
AGGGATCAGAA-3' VCAMocG sequence: 5'-
phosphate-AAATTGATTCAGGAAATACTAGCTTATAAAATGTTG ACT GGT CAC ACG
TCGCTCTGATCCC ATG ACT TCA CCT ACC AAA TAT CTA GGG ATC AGA G-3'
VCAMinA P2: CTTCACCTACCAAATATCTAGGGATCAGAA VCAMocG P21:
CTTCACCTACCAAATATCTAGGGATCAGAG P1 in Amplifluor:
5'-FAM-TCGATGACTGACGGTCATCG-Dabcyl-dT)-ACTAGAGCTGA GACATGACGAGTC-3'
P1 oc Amplifluor:
5'-TET-TCGATGACTGACGGTCATCG-(Dabcyl-dT)-ACGACGTGTG
ACCAGTCAACAT-3'
[0237] Primer (P1) is an Amplifluor and is complementary to the
region of the spacer region of an open circle probe. The sequence
of the allele-specific primer (P2) is homologous to the 3' arm of
an open circle probe. The Amplifluor P1s have either FAM or TET
fluorophores at the 5' ends for the two alleles. The Tms of both
these primers is approximately 65.degree. C.
[0238] DNA Annealing and Ligation: The reactions were set up in
96-well MicroAmp Optical plates (Perkin Elmer) in a 10 .mu.l
reaction volume containing 1 unit Ampligase (Epicentre
Technologies), 20 mM Tris-HCl (pH 8.3), 25 mM KCl, 10 mM
MgCl.sub.2, 0.5 mM AND, and 0.01% Triton.RTM. X-100. Standard
reactions contained 0.01 nM open circle probes and 100 ng of Alu I
digested genomic DNA. DNA was denatured by heating the reactions at
95.degree. C. for 3 min followed by annealing and ligation at
60.degree. C. for 30 min.
[0239] ERCA.TM. Reaction: For each 30 .mu.l reaction to be run, 20
.mu.l of ERCA mix was added to the 10 .mu.l ligation mix. ERCA mix
was prepared as follows: 3 .mu.l of 10.times. Bst Thermopol buffer
(200 mM Tris-HCl, pH 8.8, 100 mM KCl, 100 mM
(NH.sub.4).sub.2SO.sub.4 and 1% Triton X-100) containing no
Mg.sup.2+, 3 .mu.l 50 mM TMA oxalate, 1.2 .mu.l 10 mM dNTP mix
(dATP, dGTP, dCTP, and dTTP), 3 .mu.l 10 .mu.M Amplifluor P1
primer, 3 .mu.l 10 .mu.M P2 primer, 2.5 .mu.l 20 .mu.M ROX dye, 1
.mu.l of 8 units/.mu.l Bst polymerase (New England Biolabs, Mass.),
and 3.3 .mu.l water. 20 .mu.l of ERCA mix was added to the 10 .mu.l
ligation reaction. Real time ERCA reaction was performed in 96-well
MicroAmp Optical Plates (Perkin-Elmer) and run on real time ABI
Prism 7700 (Perkin-Elmer) for 3 hrs. Specific signal was expressed
as "delta Ct".multidot.Delta Ct=(Ct minus ligase control-Ct plus
ligase).
[0240] Results: Genotyping assays with the hairpin open circle
probe for the SNP VCAM gave a typical real time profiles (FIG. 5),
and 98% overall accuracy (Table 1) when 92 genomic DNA samples were
analyzed.
3 TABLE 1 Genotype Accuracy % Accuracy CT 31/31 100.0% TT (FAM-A)
55/56 98.2% CC (TET-G) 4/5 80.0% Total 90/92 98%
[0241] It is understood that the disclosed invention is not limited
to the particular methodology, protocols, and reagents described as
these may vary. It is also to be understood that the terminology
used herein is for the purpose of describing particular embodiments
only, and is not intended to limit the scope of the present
invention which will be limited only by the appended claims.
[0242] It must be noted that as used herein and in the appended
claims, the singular forms "a ", "an", and "the" include plural
reference unless the context clearly dictates otherwise. Thus, for
example, reference to "a host cell" includes a plurality of such
host cells, reference to "the antibody" is a reference to one or
more antibodies and equivalents thereof known to those skilled in
the art, and so forth.
[0243] Unless defined otherwise, all technical and scientific terms
used herein have the same meanings as commonly understood by one of
skill in the art to which the disclosed invention belongs. Although
any methods and materials similar or equivalent to those described
herein can be used in the practice or testing of the present
invention, the preferred methods, devices, and materials are as
described. Publications cited herein and the material for which
they are cited are specifically incorporated by reference. Nothing
herein is to be construed as an admission that the invention is not
entitled to antedate such disclosure by virtue of prior
invention.
[0244] Those skilled in the art will recognize, or be able to
ascertain using no more than routine experimentation, many
equivalents to the specific embodiments of the invention described
herein. Such equivalents are intended to be encompassed by the
following claims.
Sequence CWU 1
1
10 1 89 DNA Artificial Sequence Description of artificial sequence
note = synthetic construct 1 gaagaactgg acagatttac tacgtatgtt
gactggtcac acgtcgttct agtacgcttc 60 tactccctct tgagatgttc tgctttgtt
89 2 89 DNA Artificial Sequence Description of artificial sequence
note = synthetic construct 2 gaagaactgg acagatttac tacgtatgtt
gactggtcac acgtcgttct agtaacaaag 60 cactccctct tgagatgttc tgctttgtt
89 3 98 DNA Artificial Sequence Description of artificial sequence
note = synthetic construct 3 aaattgattc aggaaatact agcttataaa
gactcgtcat gtctcagctc tagtttctga 60 tcccatgact tcacctacca
aatatctagg gatcagaa 98 4 94 DNA Artificial Sequence Description of
artificial sequence note = synthetic construct 4 aaattgattc
aggaaatact agcttataaa atgttgactg gtcacacgtc gctctgatcc 60
catgacttca cctaccaaat atctagggat caga 94 5 30 DNA Artificial
Sequence Description of artificial sequence note = synthetic
construct 5 cttcacctac caaatatcta gggatcagaa 30 6 30 DNA Artificial
Sequence Description of artificial sequence note = synthetic
construct 6 cttcacctac caaatatcta gggatcagag 30 7 20 DNA Artificial
Sequence Description of artificial sequence note = synthetic
construct 7 tcgatgactg acggtcatcg 20 8 24 DNA Artificial Sequence
Description of artificial sequence note = synthetic construct 8
actagagctg agacatgacg agtc 24 9 20 DNA Artificial Sequence
Description of artificial sequence note = synthetic construct 9
tcgatgactg acggtcatcg 20 10 22 DNA Artificial Sequence Description
of artificial sequence note = synthetic construct 10 acgacgtgtg
accagtcaac at 22
* * * * *