U.S. patent application number 10/029714 was filed with the patent office on 2003-08-21 for 1,6 naphthyridines useful as inhibitors of syk kinase.
Invention is credited to Bobko, Mark A., Cywin, Charles L., Des Jarlais, Renee L., Heider, Joachim, Jakes, Scott E., Player, Mark, Rinker, James, Winters, Michael, Zhao, Bao-Ping.
Application Number | 20030158195 10/029714 |
Document ID | / |
Family ID | 21850483 |
Filed Date | 2003-08-21 |
United States Patent
Application |
20030158195 |
Kind Code |
A1 |
Cywin, Charles L. ; et
al. |
August 21, 2003 |
1,6 naphthyridines useful as inhibitors of SYK kinase
Abstract
Disclosed are compounds of formula (I): 1 wherein R.sub.3,
R.sub.5, R.sub.7 and R.sub.8 are defined herein, which are useful
as inhibitors SYK kinase and are thus useful for treating diseases
resulting from inappropriate mast cell activation, which include
allergic and inflammatory diseases. Also disclosed are
pharmaceutical compositions comprising these compounds and
processes for preparing these compounds.
Inventors: |
Cywin, Charles L.; (Bethel,
CT) ; Jakes, Scott E.; (Southbury, CT) ;
Heider, Joachim; (Warthausen, DE) ; Bobko, Mark
A.; (Exton, PA) ; Des Jarlais, Renee L.; (St.
Davids, PA) ; Player, Mark; (Phoenixville, PA)
; Rinker, James; (Reading, PA) ; Winters,
Michael; (Lancaster, PA) ; Zhao, Bao-Ping;
(West Windsor, NJ) |
Correspondence
Address: |
BOEHRINGER INGELHEIM CORPORATION
900 RIDGEBURY ROAD
P O BOX 368
RIDGEFIELD
CT
06877
US
|
Family ID: |
21850483 |
Appl. No.: |
10/029714 |
Filed: |
December 21, 2001 |
Current U.S.
Class: |
514/233.8 ;
514/253.04; 514/256; 514/300; 544/127; 544/333; 544/362;
546/122 |
Current CPC
Class: |
A61P 43/00 20180101;
A61P 29/00 20180101; C07D 471/04 20130101 |
Class at
Publication: |
514/233.8 ;
514/300; 514/256; 514/253.04; 544/127; 544/333; 544/362;
546/122 |
International
Class: |
C07D 471/02; A61K
031/5377; A61K 031/496; A61K 031/506; A61K 031/4745 |
Claims
What is claimed is:
1. A compound of formula (I) 59wherein: R.sub.3 is H,
C.sub.1-3alkyl, halogen or phenyl; R.sub.5 is N(R.sub.9 )(R.sub.10)
or OR.sub.11 , wherein R.sub.9 is H or C.sub.1-3alkyl, R.sub.10 is
aminoC.sub.2-6 alkyl, C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.10 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC.sub.1-4a- lkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, or R.sub.9 and R.sub.10 together with the
nitrogen they are bonded to may form a heterocycloalkyl group
containing one or more heteroatoms which is optionally substituted
with one or more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diCi.sub.1-4alkylamino, aminoCl.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, and R.sub.11 is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.11 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC.sub.1-4a- lkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups; R.sub.7 is phenyl, naphthyl, furyl, thienyl,
pyridyl, indolyl, benzothiazolyl or pyrrolyl optionally substituted
with one or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O,
hydroxy, C.sub.1-3alkyl, amino, C.sub.1-3alkylamino,
diC.sub.1-3alkylamino, C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, C.sub.1-3alkylcarbonyl, ureido
optionally substituted with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl,
C(O)OH, acetamido or heterocycloalkyl groups, or with a phenyl
group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diCi.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and R.sub.8 is H, halogen or
cyano. or a pharmaceutically acceptable salt thereof.
2. The compound according to claim 1 wherein: R.sub.3 is H or
methyl; R.sub.5 is N(R.sub.9)(R.sub.10 ) or OR.sub.11 wherein
R.sub.9 is H or methyl, R.sub.10 is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alky- l,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.10 is C.sub.4-7cycloalkyl, heterocycloalkyl
selected from pyrrolidinyl and piperidinyl, heteroaryl selected
from oxazolyl, isoxazolyl, pyridyl and pyrimidinyl,
C.sub.3-6cycloalkylC.sub.1-4alkyl, heterocycloalkylC.sub.1-4- alkyl
wherein said heterocycloalkyl is selected from morpholinyl,
pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, tetrahydrofuranyl and
piperazinyl, phenylC.sub.1-4alkyl or heteroarylC.sub.1-4alkyl
wherein said heteroaryl is selected from pyridyl, furyl, and
imidazolyl, each optionally substituted with one or more
C.sub.1-4alkyl, amino, C.sub.1-4alkylamino, diC.sub.1-4alkylamino,
aminoC.sub.1-3alkyl, C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, or R.sub.9 and R.sub.10 together with the
nitrogen they are bonded to may form a heterocycloalkyl group
selected from piperidinyl, piperazinyl, pyrrolidinyl and
morpholinyl which is optionally substituted with one or more
C.sub.1-4alkyl, amino, C.sub.1-4alkylamino, diC.sub.1-4alkylamino,
aminoC.sub.1-3alkyl, C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, and R.sub.11, is is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.11 is C.sub.5-7cyclohexyl, heterocycloalkyl
selected from pyrrolidinyl and piperidinyl, heteroaryl selected
from oxazolyl, isoxazolyl, pyridyl and pyrimidinyl,
C.sub.3-6cycloalkylC.sub.1- -4alkyl, heterocycloalkylC.sub.1-4alkyl
wherein said heterocycloalkyl is selected from morpholinyl,
pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, tetrahydrofuranyl and
piperazinyl, phenylC.sub.1-4alkyl or heteroarylC.sub.1-4alkyl
wherein said heteroaryl is selected from pyridyl, furyl, and
imidazolyl, each optionally substituted with one or more
C.sub.1-4alkyl, amino, C.sub.1-4alkylamino, diC.sub.1-4alkylamino,
aminoC.sub.1-3alkyl, C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups; R.sub.7 is phenyl, naphthyl, furyl, thienyl,
pyridyl, indolyl, benzothiazolyl or pyrrolyl optionally substituted
with one or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O,
hydroxy, C.sub.1-3alkyl, amino, C.sub.1-3alkylamino,
diC.sub.1-3alkylamino, C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, ureido optionally substituted
with C.sub.1-3alkyl, acetamido or heterocycloalkyl groups, or with
a phenyl group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and R.sub.8 is H; or a
pharmaceutically acceptable salt thereof.
3. The compound according to claim 1 wherein: R.sub.3 is H or
methyl; R.sub.5 is N(R.sub.9)(R.sub.10 ) or OR.sub.11 wherein
R.sub.9 is H or methyl, R.sub.10 is aminoC.sub.2-6alkyl,
methylaminoC.sub.2-6alkyl, dimethylaminoC.sub.2-6alkyl,
methoxyC.sub.2-6alkyl or hydroxyC.sub.2-6alkyl, wherein one
methylene group in said C.sub.2-6alkyl is optionally replaced with
an oxygen or sulfur, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a hydroxy group, or
R.sub.10 is C.sub.4-7cycloalkyl, heterocycloalkyl selected from
pyrrolidinyl and piperidinyl, heteroaryl selected from oxazolyl,
isoxazolyl, pyridyl and pyrimidinyl, C.sub.3-6cycloalkylC.sub.1-
-4alkyl, heterocycloalkylC.sub.1-4alkyl wherein said
heterocycloalkyl is selected from, pyrrolidin-2-onyl and
piperidinyl, benzyl or heteroarylC.sub.1-4alkyl wherein said
heteroaryl is selected from pyridyl and imidazolyl, each optionally
substituted with one or more amino, aminomethyl, methylaminomethyl,
dimethylaminomethyl, hydroxy, aminocarboxy or benzyl groups, or
R.sub.9 and R.sub.10 together with the nitrogen they are bonded to
may form a heterocycloalkyl group selected from piperidinyl,
piperazinyl, pyrrolidinyl and morpholinyl which is optionally
substituted with one or more amino, hydroxy or aminocarboxy groups,
and R.sub.11 is aminoC.sub.2-6alkyl, methylaminoC.sub.2-6alkyl,
dimethylaminoC.sub.2-6alkyl, methoxyC.sub.2-6alkyl or
hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen or sulfur, and
wherein each methylene group in said C.sub.2-6alkyl is optionally
substituted with a hydroxy group, or R.sub.11 is
C.sub.5-7cycloalkyl, heterocycloalkyl selected from pyrrolidinyl
and piperidinyl, heteroaryl selected from oxazolyl, isoxazolyl,
pyridyl and pyrimidinyl, C.sub.3-6cycloalkylC.sub.1- -4alkyl,
heterocycloalkylC.sub.1-4alkyl wherein said heterocycloalkyl is
selected from, pyrrolidin-2-onyl and piperidinyl, benzyl or
heteroarylC.sub.1-4alkyl wherein said heteroaryl is selected from
pyridyl and imidazolyl, each optionally substituted with one or
more amino, aminomethyl, hydroxy, aminocarboxy, benzyl,
methylaminomethyl, or dimethylaminomethyl groups; R.sub.7 is
phenyl, optionally substituted in the 3-, 4-or 5-positions with one
or more methoxy, fluorine, chlorine, bromine, CF.sub.3, CF.sub.3O,
C.sub.1-3alkyl, diC.sub.1-3alkylamino,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(- C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl- )amino,
thiomethoxy, acetamido, 4-morpholinyl, 1-piperidinyl or
1-pyrrolidinyl groups, or substituted in the 4-position with a
phenyl group optionally substituted in the 4-position with a
methyl, methoxy, 2-thienyl or 3-pyridyl group, or R.sub.7 is a
2-naphthyl or 2-thienyl group or a 5-indoyl group optionally
substituted in the 1-position with a CF.sub.3, C.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, or
C.sub.1-3alkylaminoC.sub.1-3alkyl group; R.sub.8 is H; or a
pharmaceutically acceptable salt thereof.
4. The compound according to claim 1 wherein: R.sub.3 is H; R.sub.5
is N(R.sub.9)(R.sub.10) or OR.sub.11 wherein R.sub.9 is H or
methyl, R.sub.10 is aminoC.sub.3-4alkyl or hydroxyC.sub.3-4alkyl
wherein each methylene group in said C.sub.3-4alkyl is optionally
substituted with a hydroxy group, or R.sub.10 is
C.sub.5-7cycloalkyl substituted with an amino or hydroxy group, and
R.sub.11 is aminoC.sub.3-4alkyl or hydroxyC.sub.3-4alkyl wherein
each methylene group in said C.sub.3-4alkyl is optionally
substituted with a hydroxy group, or R.sub.11 is
C.sub.5-7cycloalkyl substituted with an amino or hydroxy group;
R.sub.7 is phenyl, substituted in the 4-position with a
diC.sub.1-2alkylamino,
(dimethylaminoC.sub.2-3alkyl)(Co.sub.3alkyl)amino, isopropyl,
4-morpholinyl, 4-piperidinyl or 4-pyrrolidinyl group and optionally
substituted in the 3-position with a chlorine, bromine or methoxy
group; and R.sub.8 is H; or a pharmaceutically acceptable salt
thereof.
5. A compound selected from the group
6061626364656667686970717273747576o- r a pharmaceutically
acceptable salt thereof.
6. A compound selected from the group 77787980or a pharmaceutically
acceptable salt thereof.
7. A pharmaceutical composition comprising a therapeutically
effective amount of a compound according to claim 1.
8. A pharmaceutical composition comprising a therapeutically
effective amount of a compound according to claim 5.
9. A pharmaceutical composition comprising a therapeutically
effective amount of a compound according to claim 6.
10. A method for modulating the effects of a disease mediated by
SYK kinase in a warm-blooded animal comprising administering to the
animal a therapeutically effective amount of a compound as recited
in claim 1.
11. A method for modulating the effects of allergic or inflammatory
disease in a warm-blooded animal comprising administering to the
animal a therapeutically effective amount of a compound as recited
in claim 1.
12. The method according to claim 11 wherein the allergic or
inflammatory disease is selected from asthma, chronic obstructive
pulmonary disease (COPD), adult respiratory distress syndrome
(ARDS), ulcerative colitis, Crohn's disease bronchitis,
conjunctivitis, psoriasis, scleroderma, urticaria, dermatitis,
allergic rhinitis, lupus and rheumatoid arthritis.
13. A method of making a compound of formula (I) below: 81wherein
R.sub.3 is H, C.sub.1-3alkyl, halogen or phenyl; R.sub.5 is
N(R.sub.9)(R.sub.10) wherein R.sub.9 is H or C.sub.1-3alkyl;
R.sub.10 is aminoC.sub.2-6alkyl, C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.10 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC1-4alkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, or R.sub.9 and R.sub.10 together with the
nitrogen they are bonded to may form a heterocycloalkyl group
containing one or more heteroatoms which is optionally substituted
with one or more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups; R.sub.7 is phenyl, naphthyl, furyl, thienyl,
pyridyl, indolyl, benzothiazolyl or pyrrolyl optionally substituted
with one or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O,
hydroxy, C.sub.1-3alkyl, amino, C.sub.1-3alkylamino,
diC.sub.1-3alkylamino, C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-.sub.3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, C.sub.1-3alkylcarbonyl, ureido
optionally substituted with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl,
C(O)OH, acetamido or heterocycloalkyl groups, or with a phenyl
group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and R.sub.8is H; said method
comprising: (a) reacting the compound of formula (II) with a
nitrile of the formula R.sub.7CN in the presence of a strong base
in a suitable solvent to form a compound of formula (III): 82(b)
reacting the compound of formula (III) with a suitable chlorinating
agent to form the compound of formula (IV): 83(c) reacting the
compound of formula (IV) with an amine of the formula
HN(R.sub.8)(R.sub.9) to provide the desired compound of formula (I)
wherein R.sub.5 is N(R.sub.8)(R.sub.9) and R.sub.8 is H: 84
14. A method of making a compound of formula (I) below: 85wherein
R.sub.3 is H, C.sub.1-3alkyl, halogen or phenyl; R.sub.5 is
OR.sub.11 wherein R.sub.11 is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.11 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC.sub.1-4alkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups; R.sub.7 is phenyl, naphthyl, furyl, thienyl,
pyridyl, indolyl, benzothiazolyl or pyrrolyl optionally substituted
with one or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O,
hydroxy, C.sub.1-3alkyl, amino, C.sub.1-3alkylamino,
diC.sub.1-3alkylamino, C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, C.sub.1-3alkylcarbonyl, ureido
optionally substituted with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl,
C(O)OH, acetamido or heterocycloaklyl groups, or with a phenyl
group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC,.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and R.sub.8 is H; said
method comprising: (a) reacting the compound of formula (II) with a
nitrile of the formula R.sub.7CN in the presence of a strong base
in a suitable solvent to form a compound of formula (III): 86(b)
reacting the compound of formula (III) with a suitable chlorinating
agent to form the compound of formula (IV): 87(c) reacting the
compound of formula (IV) with an alcohol of the formula HO(R.sub.10
) in the presence of a suitable base in a suitable solvent to
provide the desired compound of formula (I) wherein R.sub.5 is
OR.sub.10 and R.sub.8 is H: 88
Description
TECHNICAL FIELD OF THE INVENTION
[0001] This invention relates to substituted [1,6]-naphthyridines
that inhibit SYK kinase. In one embodiment, this invention relates
to a novel class of substituted [1,6]-naphthyridines and
pharmaceutical compositions comprising these compounds. This
invention also relates to methods for producing such novel
[1,6]-naphthyridines. Because of the ability of these compounds to
inhibit SYK kinase, the compounds and pharmaceutical compositions
of this invention are particularly well suited for preventing and
treating inflammatory and allergic diseases.
BACKGROUND OF THE INVENTION
[0002] Mast cells play a critical role in asthma and allergic
disorders by releasing pro-inflammatory mediators and cytokines.
Antigen-mediated aggregation of Fc.epsilon.RI, the high-affinity
receptor for IgE results in activation of mast cells. This triggers
a series of signaling events resulting in the release of mediators,
including histamine, proteases, leukotrienes and cytokines. These
mediators cause increased vascular permeability, mucus production,
bronchoconstriction, tissue degradation and inflammation, thus
playing key roles in the etiology and symptoms of asthma and
allergic disorders.
[0003] One of the key events in the signaling pathway following the
activation of mast cells is activation of the tyrosine kinase SYK.
SYK kinase acts as a central initiator of all subsequent signaling
leading to mediator release. The critical role of SYK kinase in the
signaling path was demonstrated by the complete inhibition of
mediator release by a protein containing the SH2 domains of SYK
kinase that functioned as an inhibitor of SYK kinase (J. A. Taylor
et al., Molec. and Cell Biol., 1995, 15, 4149). Furthermore, direct
clustering of SYK, introduced into a mast cell line as part of a
chimeric transmembrane protein, was found to be sufficient to
stimulate the events leading to mediator release normally induced
by clustering of Fc.epsilon.RI (V. M. Rivera and J. S. Brugge,
Molec. and Cell. Biol., 1995, 15, 1582).
[0004] ER-27319(3,4-dimethyl-10-(3-aminopropyl)-9-acridone
oxalate), a compound reported to interfere with the activation of
SYK, has been shown to inhibit anti-IgE mediated degranulation in
rodent and human mast cells (K. Moriya et al., Proc. Nat. Acad.
Sci. USA, 1997, 94, 12539). Concentrations of piceatannol (3, 4,
3'5'-tetrahydroxy-trans-stilbene), a non-selective SYK kinase
inhibitor, that inhibited the antigen-stimulated phosphorlation of
SYK also inhibited functional responses in mast cells, including
mediator release (J. M. Oliver et. al., J. Biol. Chem., 1994, 269,
29697).
[0005] The conclusion from the studies described above is that SYK
kinase activation and activity is required for
Fc.epsilon.RI-mediated release of mediators from mast cells.
Therefore, agents that block the activity of SYK kinase act to
block the release of allergic and pro-inflammatory mediators and
cytokines. These agents have potential utility in treating
inflammatory and allergic disorders including asthma, chronic
obstructive pulmonary disease (COPD), adult respiratory distress
syndrome (ARDS), ulcerative colitis, Crohn's disease, bronchitis,
conjunctivitis, psoriasis, scleroderma, urticaria, dermatitis and
allergic rhinitis.
[0006] In addition to the inhibitors mentioned above, WO 0109134
discloses purine derivatives as inhibitors of SYK kinase. WO
9931073 describes pyrimidine-5-carboxamide derivatives as
inhibitors of SYK kinase. WO 0147922 describes substituted
azaindoles useful in the treatment of disease states capable of
being modulated by the inhibition of protein kinases, in particular
SYK kinase. WO 9818782 describes inhibitors of ZAP70 that are also
reported to inhibit SYK.
[0007] In addition to mast cells, SYK is expressed in other
hematopoietic cells including B cells, where it is thought to play
an essential role in transducing signals required for the
transition of immature B cells into mature recirculating B cells
(M. Turner et al., Immunology Today, 2000, 21, 148). B cells are
reported to play an important role in some inflammatory conditions
such as lupus (O. T. Chan et al., Immunological Rev., 1999, 169,
107) and rheumatoid arthritis (A. Gause and C. Borek, Biodrugs,
2001, 15, 73).
[0008] SYK was also reported to be an element of the signaling
cascade in beta-amyloid and prion fibrils leading to production of
neurotoxic products (C. K. Combs et al., J. Neurosci., 1999, 19,
928). Furthermore, an inhibitor of SYK blocked the production of
these neurotoxic products. Thus a SYK inhibitor would potentially
be useful in the treatment of Alzheimer's disease and related
neuroinflammatory diseases. Another report (Y. Kuno et al., Blood,
2001, 97, 1050) demonstrates that SYK plays an important role in
malignant progression. A TEL-SYK fusion protein was found to
transform hematopoietic cells suggesting a role in the pathogenesis
of hematopoietic malignancies. Therefore a SYK inhibitor may be
useful in the treatment of certain types of cancers.
[0009] A recent report suggests that SYK is a mediator of
epithelial cell growth and suggests that it could be a potential
tumor-suppressor in human breast carcinomas (P. J. P. Coopman et
al., Nature, 2000, 406, 742). One could conclude from this report
that while inhibition of SYK kinase activity could be desirable for
treatment of inflammatory and allergic disease and asthma, a
complete, irreversible blockade of SYK kinase activity may not be
desirable.
[0010] BE 835,770 describes 5-amino-1,6-naphthyridines having
antimicrobial activity. U.S. Pat. No. 3,928,367, U.S. Pat. No.
4,017,500, U.S. Pat. No. 4,115,395 and U.S. Pat. No. 4,260,759
describe 5-amino-1,6-naphthyridines having antifungal and
antibacterial activity. WO 9918077 describes
5-piperazinyl-1,6-naphthyridines as serotonin antagonists.
BRIEF DESCRIPTION OF THE INVENTION
[0011] The work cited above supports the principle that inhibition
of SYK kinase will be beneficial in the treatment of various
disease states. It is therefore an object of the invention to
provide novel compounds which inhibit SYK kinase
[0012] It is a further object of the invention to provide methods
for treating diseases and pathological conditions mediated by SYK
kinase such as inflammatory and allergic disorders including
asthma, chronic obstructive pulmonary disease (COPD), adult
respiratory distress syndrome (ARDS), ulcerative colitis, Crohn's
disease, bronchitis, conjunctivitis, psoriasis, scleroderma,
urticaria, dermatitis allergic rhinitis, lupus and rheumatoid
arthritis, using the novel compounds of the invention.
[0013] It is yet a further object of the invention to provide
processes of preparation for the above-mentioned novel compounds
and pharmaceutical compositions comprising the same.
DETAILED DESCRIPTION OF THE INVENTION
[0014] In its broadest generic aspect, the invention provides novel
compounds of the formula (I) below: 2
[0015] wherein:
[0016] R.sub.3 is H, C.sub.1-3alkyl, halogen or phenyl;
[0017] R.sub.5 is N(R.sub.9 )(R.sub.10) or OR.sub.11 , wherein
[0018] R.sub.9 is H or C.sub.1-3alkyl,
[0019] R.sub.10 is aminoC.sub.2-6 alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl- ,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.10 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC.sub.1-4alkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, or R.sub.9 and R.sub.10 together with the
nitrogen they are bonded to may form a heterocycloalkyl group
containing one or more heteroatoms which is optionally substituted
with one or more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diCi.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, and
[0020] R.sub.11 is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylamninoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.11 is C.sub.3-7cycloalkyl,
heterocycloalkyl, heteroaryl, C.sub.3-7cycloalkylC.sub.1-4alkyl,
heterocycloalkylC.sub.1-4alkyl, arylC.sub.1-4alkyl or
heteroarylC.sub.1-4alkyl each optionally substituted with one or
more C.sub.1-4alkyl, amino, C.sub.1-4alkylamino,
diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups;
[0021] R.sub.7 is phenyl, naphthyl, furyl, thienyl, pyridyl,
indolyl, benzothiazolyl or pyrrolyl optionally substituted with one
or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, C.sub.1-3alkylcarbonyl, ureido
optionally substituted with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl,
C(O)OH, acetamido or heterocycloaklyl groups, or with a phenyl
group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diCi.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and
[0022] R.sub.8 is H, halogen or cyano.
[0023] In another embodiment of the invention, there are provided
compounds of the formula (I) as described immediately above, and
wherein:
[0024] R.sub.3 is H or methyl;
[0025] R.sub.5 is N(R.sub.9)(R.sub.10) or OR.sub.11 wherein
[0026] R.sub.9 is H or methyl,
[0027] R.sub.10 is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alkyl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.10 is C.sub.4-7cycloalkyl, heterocycloalkyl
selected from pyrrolidinyl and piperidinyl, heteroaryl selected
from oxazolyl, isoxazolyl, pyridyl and pyrimidinyl, C.sub.3-6
cycloalkylC.sub.1-4alkyl, heterocycloalkylC.sub.1-- 4alkyl wherein
said heterocycloalkyl is selected from morpholinyl, pyrrolidinyl,
pyrrolidin-2-onyl, piperidinyl, tetrahydrofuranyl and piperazinyl,
phenylC.sub.1-4alkyl or heteroarylC.sub.1-4alkyl wherein said
heteroaryl is selected from pyridyl, furyl, and imidazolyl, each
optionally substituted with one or more C.sub.1-4alkyl, amino,
C.sub.1-4alkylamino, diC.sub.1-4alkylamino, aminoC.sub.1-3alkyl,
C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, or R.sub.9 and R.sub.10 together with the
nitrogen they are bonded to may form a heterocycloalkyl group
selected from piperidinyl, piperazinyl, pyrrolidinyl and
morpholinyl which is optionally substituted with one or more
C.sub.1-4alkyl, amino, C.sub.1-4alkylamino, diC.sub.1-4alkylamino,
aminoC.sub.1-3alkyl, C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups, and
[0028] R.sub.11 is is aminoC.sub.2-6alkyl,
C.sub.1-4alkylaminoC.sub.2-6alk- yl,
diC.sub.1-4alkylaminoC.sub.2-6alkyl, C.sub.1-4alkoxyC.sub.2-6alkyl
or hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen, sulfur, NH,
or NCH.sub.3, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a halogen, cyano or
hydroxy group, or R.sub.11 is C.sub.5-7cyclohexyl, heterocycloalkyl
selected from pyrrolidinyl and piperidinyl, heteroaryl selected
from oxazolyl, isoxazolyl, pyridyl and pyrimidinyl,
C3-6cycloalkylC.sub.1-4alkyl, heterocycloalkylC.sub.1-4alkyl
wherein said heterocycloalkyl is selected from morpholinyl,
pyrrolidinyl, pyrrolidin-2-onyl, piperidinyl, tetrahydrofuranyl and
piperazinyl, phenylC.sub.1-4alkyl or heteroarylC.sub.1-4alkyl
wherein said heteroaryl is selected from pyridyl, furyl, and
imidazolyl, each optionally substituted with one or more
C.sub.1-4alkyl, amino, C.sub.1-4alkylamino, diC1-4alkylamino,
aminoC.sub.1-3alkyl, C.sub.1-4alkylaminoC.sub.1-3alkyl,
diC.sub.1-4alkylaminoC.sub.1-3alkyl, halogen, hydroxy, aminocarboxy
or benzyl groups;
[0029] R.sub.7 is phenyl, naphthyl, furyl, thienyl, pyridyl,
indolyl, benzothiazolyl or pyrrolyl optionally substituted with one
or more C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl)amino,
C.sub.1-3alkylthio, aminocarboxy, ureido optionally substituted
with C.sub.1-3alkyl, acetamido or heterocycloalkyl groups, or with
a phenyl group which is optionally substituted with one or more
C.sub.1-3alkoxy, halogen, CF.sub.3, CF.sub.3O, hydroxy,
C.sub.1-3alkyl, amino, C.sub.1-3alkylamino, diC.sub.1-3alkylamino,
C.sub.1-3alkylaminoC.sub.1-3a- lkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, C.sub.1-3alkylthio,
aminocarboxy, C.sub.1-3alkylcarbonyl, ureido optionally substituted
with C.sub.1-3alkyl, C(O)OC.sub.1-4alkyl, C(O)OH, acetamido,
heteroaryl or heterocycloalkyl groups; and
[0030] R.sub.8 is H.
[0031] In another embodiment of the invention there are provided
compounds of the formula (I) as described immediately above, and
wherein:
[0032] R.sub.5 is N(R.sub.9)(R.sub.10) or OR.sub.11 wherein
[0033] R.sub.9 is H or methyl,
[0034] R.sub.10 is aminoC.sub.2-6alkyl, methylaminoC.sub.2-6alkyl,
dimethylaminoC.sub.2-6alkyl, methoxyC.sub.2-6alkyl or
hydroxyC.sub.2-6alkyl, or hydroxyC.sub.2-6alkyl, wherein one
methylene group in said C.sub.2-6alkyl is optionally replaced with
an oxygen or sulfur, and wherein each methylene group in said
C.sub.2-6alkyl is optionally substituted with a hydroxy group, or
R.sub.10 is C.sub.4-7cycloalkyl, heterocycloalkyl selected from
pyrrolidinyl and piperidinyl, heteroaryl selected from oxazolyl,
isoxazolyl, pyridyl and pyrimidinyl,
C.sub.3-6cycloalkylC.sub.1-4alkyl, heterocycloalkylC.sub.1-4- alkyl
wherein said heterocycloalkyl is selected from, pyrrolidin-2-onyl
and piperidinyl, benzyl or heteroarylC.sub.1-4alkyl wherein said
heteroaryl is selected from pyridyl and imidazolyl, each optionally
substituted with one or more amino, aminomethyl, methylaminomethyl,
dimethylaminomethyl, hydroxy, aminocarboxy or benzyl groups, or
R.sub.9 and R.sub.10 together with the nitrogen they are bonded to
may form a heterocycloalkyl group selected from piperidinyl,
piperazinyl, pyrrolidinyl and morpholinyl which is optionally
substituted with one or more amino, hydroxy or aminocarboxy groups,
and
[0035] R.sub.11 is aminoC.sub.2-6alkyl, methylaminoC.sub.2-6alkyl,
dimethylaminoC.sub.2-6alkyl, methoxyC.sub.2-6alkyl or
hydroxyC.sub.2-6alkyl, wherein one methylene group in said
C.sub.2-6alkyl is optionally replaced with an oxygen or sulfur, and
wherein each methylene group in said C.sub.2-6alkyl is optionally
substituted with a hydroxy group, or R.sub.11 is
C.sub.5-7cycloalkyl, heterocycloalkyl selected from pyrrolidinyl
and piperidinyl, heteroaryl selected from oxazolyl, isoxazolyl,
pyridyl and pyrimidinyl, C.sub.3-6cycloalkylC.sub.1- -4alkyl,
heterocycloalkylC.sub.1-4alkyl wherein said heterocycloalkyl is
selected from, pyrrolidin-2-onyl and piperidinyl, benzyl or
heteroarylC.sub.1-4alkyl wherein said heteroaryl is selected from
pyridyl and imidazolyl, each optionally substituted with one or
more amino, aminomethyl, hydroxy, aminocarboxy, benzyl,
methylaminomethyl, or dimethylarninomethyl groups;
[0036] R.sub.7 is phenyl, optionally substituted in the 3-, 4-or
5-positions with one or more methoxy, fluorine, chlorine, bromine,
CF.sub.3, CF.sub.3O, C.sub.1-3alkyl, diC.sub.1-3alkylamino,
diC.sub.1-3alkylaminoC.sub.1-3alkyl,
(C.sub.1-3alkylaminoC.sub.1-3alkyl)(- C.sub.0-3alkyl)amino,
(diC.sub.1-3alkylaminoC.sub.1-3alkyl)(C.sub.0-3alkyl- )amino,
thiomethoxy, acetamido, 4-morpholinyl, 1-piperidinyl or
1-pyrrolidinyl groups, or substituted in the 4-position with a
phenyl group optionally substituted in the 4-position with a
methyl, methoxy, 2-thienyl or 3-pyridyl group, or R.sub.7 is a
2-naphthyl or 2-thienyl group or a 5-indoyl group optionally
substituted in the 1-position with a CF.sub.3, C.sub.1-3alkyl,
diC.sub.1-3alkylaminoC.sub.1-3alkyl, or
C.sub.1-3alkylaminoC.sub.1-3alkyl group; and
[0037] R.sub.8 is H.
[0038] In yet another embodiment of the invention there are
provided compounds of the formula (I) as described immediately
above, and wherein:
[0039] R.sub.3 is H;
[0040] R.sub.5 is N(R.sub.9)(R.sub.10) or OR.sub.11 wherein
[0041] R.sub.9 is H or methyl,
[0042] R.sub.10 is aminoC.sub.3-4alkyl or hydroxyC.sub.3-4alkyl
wherein each methylene group in said C.sub.3-4alkyl is optionally
substituted with a hydroxy group, or R.sub.10 is
C.sub.5-7cycloalkyl substituted with an amino or hydroxy group,
and
[0043] R.sub.11 is aminoC.sub.3-4alkyl or hydroxyC.sub.3-4alkyl
wherein each methylene group in said C.sub.3-4alkyl is optionally
substituted with a hydroxy group, or R.sub.11 is
C.sub.5-7cycloalkyl substituted with an amino or hydroxy group;
[0044] R.sub.7 is phenyl, substituted in the 4-position with a
diC.sub.1-2alkylamino,
(dimethylaminoC.sub.2-3alkyl)(C.sub.0-3alkyl)amino- , isopropyl,
4-morpholinyl, 4-piperidinyl or 4-pyrrolidinyl group and optionally
substituted in the 3-position with a chlorine, bromine or methoxy
group; and
[0045] R.sub.8 is H.
[0046] In a further embodiment of the invention, there are provided
the following compounds: 3456789101112131415161718192021
[0047] In another embodiment of the invention, there are provided
the following compounds: 2223242526
[0048] The invention includes pharmaceutically acceptable
derivatives of compounds of formula (I). A "pharmaceutically
acceptable derivative" refers to any pharmaceutically acceptable
acid, salt or ester of a compound of this invention, or any other
compound which, upon administration to a patient, is capable of
providing (directly or indirectly) a compound of this invention, a
pharmacologically active metabolite or pharmacologically active
residue thereof.
[0049] Pharmaceutically acceptable salts of the compounds of this
invention include those derived from pharmaceutically acceptable
inorganic and organic acids and bases. Examples of suitable acids
include hydrochloric, hydrobromic, sulfuric, nitric, perchloric,
fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic,
toluene-p-sulfonic, tartaric, acetic, citric, methanesulfonic,
formic, benzoic, malonic, naphthalene-2-sulfonic and
benzenesulfonic acids. Other acids, such as oxalic acid, while not
themselves pharmaceutically acceptable, may be employed in the
preparation of salts useful as intermediates in obtaining the
compounds of this invention and their pharmaceutically acceptable
acid addition salts. Salts derived from appropriate bases include
alkali metal (e.g., sodium), alkaline earth metal (e.g.,
magnesium), ammonium and N--(C.sub.1-C.sub.4 alkyl).sub.4.sup.+
salts.
[0050] In addition, the compounds of this invention include
prodrugs of compounds of the formula (I). Prodrugs include those
compounds that, upon simple transformation, are modified to produce
the compounds of the invention. Simple chemical transformations
include hydrolysis, oxidation and reduction which occur
enzymatically, metabolically or otherwise. Specifically, when a
prodrug of this invention is administered to a patient, the prodrug
may be transformed into a compound of formula (I), thereby
imparting the desired pharmacological effect.
[0051] Any compounds of this invention containing one or more
asymmetric carbon atoms may occur as racemates and racemic
mixtures, single enantiomers, diastereomeric mixtures and
individual diastereomers. All such isomeric forms of these
compounds are expressly included in the present invention. Each
stereogenic carbon may be in the R or S configuration, or a
combination of configurations.
[0052] Some of the compounds of the invention can exist in more
than one tautomeric form. The invention includes all such
tautomers.
[0053] The compounds of the invention are only those which are
contemplated to be `chemically stable` as will be appreciated by
those skilled in the art. For example, a compound which would have
a `dangling valency`, or a `carbanion` are not compounds
contemplated by the invention.
[0054] As used herein, the following abbreviations are used:
[0055] BuOH is butanol;
[0056] DMF is dimethylformamide;
[0057] DMSO is dimethyl sulfoxide
[0058] EtOAc is ethyl acetate;
[0059] EtOH is ethanol;
[0060] HPLC is high-performance liquid chromatography
[0061] LDA is lithium diisopropylamide;
[0062] MeOH is methanol;
[0063] THF is tetrahydrofuran;
[0064] TLC is thin layer chromatography
[0065] All terms as used herein in this specification, unless
otherwise stated, shall be understood in their ordinary meaning as
known in the art. For example, "C.sub.1-6alkoxy" is a
C.sub.1-6alkyl with a terminal oxygen, such as methoxy, ethoxy,
propoxy, pentoxy and hexoxy. All alkyl, alkylene or alkynyl groups
shall be understood as being branched or unbranched unless
otherwise specified. Other more specific definitions are as
follows:
[0066] The term "alkyl" refers to a saturated aliphatic radical
containing from one to ten carbon atoms or a mono- or
polyunsaturated aliphatic hydrocarbon radical containing from two
to twelve carbon atoms unless otherwise stated. The mono- or
polyunsaturated aliphatic hydrocarbon radical contains at least one
double or triple bond, respectively. "Alkyl" refers to both
branched and unbranched alkyl groups. Examples of "alkyl" include
alkyl groups which are straight chain alkyl groups containing from
one to eight carbon atoms and branched alkyl groups containing from
three to ten carbon atoms. Other examples include lower alkyl
groups which are straight chain alkyl groups containing from one to
six carbon atoms and branched alkyl groups containing from three to
six carbon atoms. It should be understood that any combination term
using an "alk" or "alkyl" prefix refers to analogs according to the
above definition of "alkyl". For example, terms such as "alkoxy",
"alkythio" refer to alkyl groups linked to a second group via an
oxygen or sulfur atom. "Alkanoyl" refers to an alkyl group linked
to a carbonyl group (C.dbd.O). Each alkyl or alkyl analog described
herein shall be understood to be optionally partially or fully
halogenated.
[0067] The term "cycloalkyl" refers to the cyclic analog of an
alkyl group, as defined above. Examples of cycloalkyl groups are
saturated or unsaturated nonaromatic cycloalkyl groups containing
from three to eight carbon atoms, and other examples include
cycloalkyl groups having three to six carbon atoms.
[0068] The term "heterocycloalkyl" refers to a stable 4-8 membered
(but preferably, 5 or 6 membered) monocyclic or 8-11 membered
bicyclic heterocycle radical which may be either saturated or
unsaturated, and is non-aromatic. Each heterocycle consists of
carbon atoms and from 1 to 4 heteroatoms chosen from nitrogen,
oxygen and sulfur. The heterocycle may be attached by any atom of
the cycle, which results in the creation of a stable structure.
Examples of "heterocycloalkyl" include radicals such as pyrrolinyl,
pyrrolidinyl, pyrazolinyl, pyrazolidinyl, piperidinyl, morpholinyl,
thiomorpholinyl, piperazinyl, indolinyl, azetidinyl,
tetrahydropyranyl, tetrahydrothiopyranyl, tetrahydrofuranyl,
hexahydropyrimidinyl, hexahydropyridazinyl, dihydro-oxazolyl,
1,2-thiazinanyl-1,1-dioxide, 1,2,6-thiadiazinanyl-1,1-dioxide,
isothiazolidinyl-1,1-dioxide and imidazolidinyl-2,4-dione.
[0069] The term "halogen" refers to bromine, chlorine, fluorine or
iodine.
[0070] The term "aryl" shall be understood to mean a 6-12 membered
aromatic carbocycle, which can be a single ring or can be multiple
rings fused together or linked covalently. The term "aryl"
includes, for example, phenyl and naphthyl; other terms comprising
"aryl" will have the same definition for the aryl component,
examples of these moieties include: arylalkyl, aryloxy or
arylthio.
[0071] The term "heteroaryl" refers to a stable 5-8 membered (but
preferably, 5 or 6 membered) monocyclic or 8-11 membered bicyclic
aromatic heterocycle radical. Each heterocycle consists of carbon
atoms and from 1 to 4 heteroatoms chosen from nitrogen, oxygen and
sulfur. The heteroaryl group may be attached by any atom of the
ring which results in the creation of a stable structure. Examples
of "heteroaryl" include radicals such as furanyl, thienyl,
pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl,
isothiazolyl, oxadiazolyl, triazolyl, tetrazolyl, thiadiazolyl,
pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl,
indolyl, isoindolyl, benzofuranyl, benzothienyl, indazolyl,
benzimidazolyl, benzthiazolyl, benzoxazolyl, purinyl, quinolizinyl,
quinolinyl, isoquinolinyl, cinnolinyl, phthalazinyl, quinazolinyl,
quinoxalinyl, naphthyridinyl, pteridinyl, carbazolyl, acridinyl,
phenazinyl, phenothiazinyl and phenoxazinyl,
[0072] As used herein above and throughout this application,
"nitrogen" and "sulfur" include any oxidized form of nitrogen and
sulfur and the quatemized form of any basic nitrogen.
[0073] General Synthetic Methods
[0074] The invention additionally provides for methods for making
the compounds of the formula (I).
[0075] The compounds of the invention may be prepared by the
general methods and examples presented below, and methods known to
those of ordinary skill in the art.
[0076] Reaction progress may be monitored by conventional methods
such as thin layer chromatography (TLC). Intermediates and products
may be purified by methods known in the art, including column
chromatography, HPLC or recrystallization.
[0077] As illustrated in Scheme I, a 2-methylnicotinic acid
derivative (II) is treated with a strong base such as lithium
diisopropylamide (LDA) in a suitable solvent such as THF at about
-78.degree. C. to 0.degree. C. to form the dianion. This is
followed by addition of a nitrile bearing the desired R.sub.7,
preferably at about -78.degree. C., and allowing the reaction to
warm to about room temperature until reaction is complete to
provide the [1,6]-naphthyridin-5-ol (III), which may also exist as
the tautomeric 6H-[1,6]-naphthyridin-5-one (IIIa).
[0078] The [1,6]-naphthyridin-5-ol is then treated with a suitable
halogenating agent. For example, treatment with POCl.sub.3,
optionally in the presence of a suitable base such as
N,N-diethylaniline, at a temperature of about 0.degree. C. to
140.degree. C., preferably at about 100.degree. C. to 135.degree.
C., provides the 5chloro-[1,6]-naphthyridin- e IV. This may then be
reacted with an excess of the desired amine (R'NH.sub.2) at a
suitable temperature, preferably about 100.degree. C. to
140.degree. C., optionally in a sealed reaction vessel, to provide
a compound of formula (I) having an amine at R.sub.5. If a compound
of formula (I) having an ether link at R.sub.5 is desired, one may
react IV with the desired alcohol (R'OH), in the presence of a
suitable base such as sodium hydride in a suitable solvent such as
DMF at a temperature of about 0.degree. C. to 100.degree. C.,
preferably at about room temperature. 27
[0079] If a compound of formula (I) having R.sub.8 being a
substituent other than hydrogen is desired, one may react an
intermediate of formula (III) with a halogenating agent, preferably
N-iodosuccinamide, in a suitable solvent such as DMF, at about room
temperature, to provide the 8-iodo intermediate V (Scheme II). The
8-iodo substituent may then be converted to other desired R.sub.8
by methods known to those skilled in the art. Likewise, R.sub.5 or
R.sub.7 may be reacted further by methods known to those skilled in
the art to prepare additional compounds of formula (I). Several
illustrative examples are provided in the Experimental section.
28
[0080] The [1,6]-naphthyridin-5-ol III may also be prepared by an
alternative procedure illustrated in Scheme III (Method E). 29
[0081] In this procedure, described in the literature (D. E. Ames
and W. D. Dodds, J. C. S. Perkin I, 1972, 705), a carbanion is
reacted with a 2-halonicotinic acid, preferably a 2-chloro- or
2-bromonicotinic acid, (VI), in the presence of a copper catalyst,
preferably copper acetate, to give a
2-(2-substituted-2-oxo-ethyl)nicotinic acid intermediate (VIII).
The carbanion may be generated from a 1,3-dicarbonyl compound such
as VII, and a base, preferably sodium ethoxide. Subsequent
ethanolysis cleaves the acetyl group to provide VIII. Treatment of
VIII with ammonia in a suitable solvent, such as dioxane or
ethanol, at a suitable temperature, preferably the reflux
temperature of the solvent, provides III.
[0082] In a variation of Method E illustrated in Scheme IV (Method
F), intermediate VIII may be treated with aqueous acid, such as
aqueous sulfuric acid, at about room temperature to provide a
pyrano[4,3-b]pyridin-5-one (IX). This may then be treated with
ammonia in a suitable solvent such as ethanol, at a suitable
temperature, preferably about the reflux temperature, to provide
III. 30
SYNTHETIC EXAMPLES
[0083] The following examples are for the purpose of illustrating
preferred embodiments of this invention, and are not to be
construed as limiting the scope of the invention in any way.
[0084] 7-Aryl-5-amino-[1,6]naphthyridines may be made by the
procedure described in Examples 1-3 below (Methods A-C). By
choosing the appropriately substituted aryl nitrile (Example 1) and
appropriately substituted amine (Example 3) or alcohol (Example 11)
one may obtain the desired compound of formula (I). Alternatively,
one may obtain a precursor of the desired compound, which may be
further modified synthetically by methods known to those skilled in
the art to obtain the desired compound of formula (I).
EXAMPLE 1
[0085] 7-(4-Dimethylaminophenyl)-[1,6]naphthyridin-5-ol (Method A)
31
[0086] To a suspension of 2-methylnicotinic acid (1.37 g, 10 mmol)
in THF (20 mL), lithium diisopropylamide (2 M solution in THF, 12.5
mL, 25 mmol) was added dropwise at -78.degree. C. The resulting
mixture was stiffed at -78.degree. C. for 30 minutes then slowly
warmed up to 0.degree. C. over 1.5 hours. The mixture was cooled to
-78.degree. C. and a solution of 4-dimethylaminophenylnitrile (2.19
g, 15 mmol) in THF (10 mL) was added dropwise. The mixture was
slowly warmed to room temperature and stirred for 16 hours. Water
(20 mL) was added and the THF was removed in vacuo. EtOAc (10 mL)
was added and the solution was allowed to stand for two hours. The
precipitated solid was collected by filtration to afford the title
compound as a yellow solid (1.2 g). .sup.1H NMR (CDCl.sub.3, 400
MHz) .delta.11.58 (b, 1H),8.86 (dd, 1H), 8.45 (d, 1H), 6.86 (d,
1H), 3.92 (s, 3H), 3.88 (s, 3H), 2.87 (s, 6H), MS (m/e) calculated
for C.sub.15H.sub.15N.sub.3O 265, found 266.2 (M+H).
EXAMPLE 2
[0087] 5-Chloro-7-(4-dimethylaminophenyl)-[1,6]naphthyridine
(Method B) 32
[0088] A mixture of
7-(4-dimethylaminophenyl)-[1,6]naphthyridin-5-ol (Example 1) (1.2
g, 5 mmol), POCl.sub.3 (10 mL) and N,N-diethylaniline (0.15 g, 1.0
mmol) were stirred at 110.degree. C. for 16 hours. The contents
were cooled to room temperature and excess POCl.sub.3 was removed
in vacuo. The residue was quenched with water and neutralized with
aqueous sodium carbonate. The aqueous solution was then extracted
with dichloromethane (2.times.30 mL). The combined organic extracts
were dried over anhydrous magnesium sulfate and concentrated in
vacuo to give a tan oil. Purification by flash chromatography
eluting with hexanes/EtOAc (1:1) afforded the title compound as a
yellowish solid (1.1 g). .sup.1H NMR (CDCl.sub.3, 400 MHz)
.delta.9.03 (dd, 1H), 8.54 (d, 1H), 8.13 (s, 1H), 8.08 (d, 2H),
7.46 (dd, 1H), 6.81 (d, 2H) 3.05 (s, 6H).
EXAMPLE 3
[0089]
5-(3-Aminopropylamino)-7-(4-dimethylaminophenyl)-[1,6]naphthyridine
(Method C) 33
[0090] A mixture of
5-chloro-7-(4-dimethylaminophenyl)-[1,6]naphthyridine (Example 2)
(10 mg, 0.035 mmol) and 1,3-diaminopropane (200 microL, 2.4 mmol)
was stirred at 100.degree. C. for 5 hours. After cooling to room
temperature, water was added and the resulting mixture was
extracted with dichloromethane (2.times.3 mL). Purification by
preparative thin layer chromatography afforded the title compound
as a yellow film (8.5 mg). .sup.1H NMR (CDCl.sub.3, 400 MHz)
.delta.8.85 (dd, 1H), 8.10 (d, 2H), 8.07 (d, 1H), 7.50 (s, 1H),
7.21 (dd, 1H), 6.81 (d, 2H), 3.87 (t, 2H), 3.02 (s, 6H), 2.97 (t,
2H), 1.90 (quint, 2H); MS (m/e) calculated for
C.sub.19H.sub.23N.sub.5 321.2, found 322.4 (M+H).
[0091] Examples 4-13 are illustrative of procedures that may be
used to modify intermediates to obtain desired compounds of the
invention.
EXAMPLE 4
[0092] 5-Chloro-7-(3-hydroxyphenyl)-[1,6]naphthyridine 34
[0093] To a solution of
5-chloro-7-(3-methoxyphenyl)-[1,6]naphthyridine (0.052 g, 0.2 mmol)
in dichloromethane (2 mL) was added boron tribromide (1 M solution
in dichloromethane, 0.4 mL, 0.4 mmol) at -78.degree. C. The
resulting mixture was slowly warmed to room temperature. The
residue was quenched with water and neutralized with aqueous sodium
carbonate. The aqueous solution was then extracted with
dichloromethane (2.times.30 mL). The combined organic extracts were
dried over anhydrous magnesium sulfate and concentrated in vacuo to
give a tan oil. Purification by flash chromatography eluting with
hexanes/EtOAc (1:1) afforded the title compound as a yellowish
solid (0.040 g). .sup.1H NMR (DMSO-d6, 400 MHz) .delta.9.70 (b,
1H), 9.19 (dd, 1H), 8.54-8.64 (m, 1H), 8.37 (s, 1H), 7.77(dd, 1H),
7.64 (m, 2H), 7.32 (t, 1H), 6.87 (m, 1H); MS (m/e) calculated for
C.sub.16H.sub.13ClN.sub.2O 256, found 257.5 (M+H).
EXAMPLE 5
[0094] 7-(4'-Methoxybiphenyl-4-yl)-[1,6]naphthyridin-5-ol 35
[0095] To a suspension of 7-(4-bromophenyl)-[1,6]-naphthyridin-5-ol
(0.135 g, 0.45 mmol)) in DMF (4 mL), was added
tetrakis(triphenylphosphine)palla- dium (0.025 g, 0.02 mmol). After
15 minutes, 4-methoxyphenylboronic acid (0.090 g, 0.60 mmol) and
sodium carbonate (2 M aqueous solution, 0.5 mL, 1 mmol) was added.
The mixture was heated to 100.degree. C. for 12 hours and then
cooled to room temperature. The white solid was collected by
filtration and washed with water to afford the title compound
(0.110 g).
EXAMPLE 6
[0096]
5-(3-Aminopropylamino)-7-[6-dhnethylamino-biphenyl-3-yl)-[1,6]napht-
hyridine 36
[0097] A mixture of 4-dimethylaminobenzonitrile (2.92 g, 20 mmol)
and N-bromosuccinmide (3.56 g, 20 mmol) in dichloromethane (50 mL)
was stirred at room temperature overnight. The solution was washed
with water (20 mL), dried over magnesium sulfate and concentrated
to afford 3-bromo-4-dimethylaminobenzonitrile as a white solid
(4.27 g).
[0098] Treatment of the above intermediate with 2-methylnicotinic
acid according to Method A (Example 1) provided the corresponding
[1,6]napthyridin-5-ol. Treatment of this with phenylboronic acid
using the procedure described in Example 5 provided
7-(6-dimethylamino-biphenyl- -3-yl)-[1,6]naphthyridin-5-ol.
Conversion to the 5-chloro intermediate using Method B, followed by
treatment of this with 1,3-diaminopropane by the procedure
described in Method C (Example 3) provided the title compound.
.sup.1H NMR (CDCl.sub.3, 400 MHz) .delta.8.82 (dd, 1H), 8.16 (d,
1H), 8.04 (dd, 1H), 8.00 (d, 1H), 7.63 (dd, 2H), 7.52 (s, 1H), 7.41
(t, 2H) 7.29 (t, 1H), 7.19 (dd, 1H), 7.08 (d, 1H), 3.84 (t, 2H),
3.00 (t, 2H), 2.58 (s, 6H), 1.99 (t, 2H); MS (m/e) calculated for
C.sub.25H.sub.27N.sub.5 397, found 398.3 (M+H).
EXAMPLE 7
[0099]
5-(3Aminopropylamino)-7(3-chloro-4diethylaminophenyl)-[1,6]naphthyr-
idine 37
[0100] A sealed tube with 3-chloro-4-fluorobenzonitrile (0.366 g,
2.4 mmol) and diethylamine (0.6 mL, 5.8 mmol) was heated to
100.degree. C. for 8 hours. The reaction was allowed to cool to
room temperature and water (5 mL) was added. The solution was
extracted with dichloromethane (2.times.5 mL), dried over magnesium
sulfate and concentrated in vacuo to afford
3-chloro-4-diethylaminobenzonitrile as an oil (0.38 g). This was
reacted as described in Methods A-C (Examples 1-3) to provide the
title compound. .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta.8.89 (dd,
1H), 8.26 (d, 1H), 8.11 (ddd, 1H), 7.97 (dd, 2.2 Hz, 1H), 7.53 (d,
1H), 7.27 (dd, 1H), 7.15 (d, 1H), 3.86 (b, 2H), 3.20 (q, 4H), 3.03
(t, 2H), 1.91 (quint, 2H), 1.08 (t, 6H); MS (m/e) calculated for
C.sub.21 H.sub.26ClN.sub.5 383, found 384.3 (M+H).
EXAMPLE 8
[0101]
5-(3Aminopropylamino)-7-(3-methoxy-4-dimethylaminophenyl)-[1,6]naph-
thyridine 38
[0102] To a solution of 3-methoxy-4-aminobenzoic acid (1.5 g, 9
mmol) in DMF (10 mL) was added sodium hydride (60% in mineral oil,
1.5 g, 37.5 mmol) at 0.degree. C. in portions. After stirring for
30 minutes, methyl iodide (6 g, 45 mmol) was added. The mixture was
stirred overnight and quenched by addition of water. The aqueous
mixture was extracted with dichloromethane (2.times.20 mL), dried
over sodium sulfate and concentrated in vacuo. Purification by
flash chromatography eluting with hexane/EtOAc (4:1) afforded
3-methoxy-4-dimethylaminobenzoic acid as an oil (1.8 g).
[0103] A suspension of the above intermediate (1.20 g, 5.7 mmol)
and sodium amide (1.0 g, 25 mmol) in toluene (20 mL) was heated to
108.degree. C. for 16 hours. The solution was allowed to cool to
room temperature, water was added dropwise, and the resulting
mixture was extracted with EtOAc (2.times.20 mL). The combined
organic extracts were dried over sodium sulfate and concentrated in
vacuo. Purification by flash chromatography eluting with hexane and
EtOAc (1:1) afforded 3-methoxy-4-dimethylaminobenzamide as an oil
(0.35 g).
[0104] A mixture of the above benzamide (0.220 g, 1.25 mmol),
POCl.sub.3 (1 mL) and diisopropylethylamine (0.5 mL) was heated to
105.degree. C. for 16 hours and then cooled to room temperature.
The solution was poured into ice water and neutralized with sodium
carbonate, then extracted with EtOAc (2.times.20 mL). The combined
organic extracts were dried over sodium sulfate and concentrated in
vacuo. Purification by flash chromatography eluting with
hexanes/EtOAc (2:1) afforded 3-methoxy-4-dimethylaminobenzonitrile
as an oil (0.20 g).
[0105] The above benzonitrile was reacted as described in Methods
A-C (Examples 1-3) to provide the title compound. .sup.1H NMR
(CD.sub.3OD) .delta.8.88 (b, 1H), 8.13 (d, 1H), 7.74-7.77 (m, 2H),
7.56 (s, 1H), 7.21 (dd, 1H), 7.08 (b, 1H), 7.02 (d, 1H), 4.00 (s,
3H), 3.88 (b, 2H), 2.85 (s, 6H), 1.96 (b, 2H); MS (m/e) calculated
for C.sub.20H.sub.25N.sub.5O 351, found 352 (M+H).
EXAMPLE 9
[0106]
5-(3-Aminopropylamino)-7(4-diethylaminophenyl)-3-methyl-[1,6]naphth-
yridine 39
[0107] To a solution of N,N-diethyl-2,5-dichloronicotinamide (0.984
g, 4.0 mmol) in THF (15 mL) was added palladium acetate (12 mg,
0.05 mmol) and 2-(di-t-butylphosphino)biphenyl (28 mg, 0.09 mmol).
After the mixture was stirred for 5 minutes, methylzinc chloride (2
M solution in dichloromethane, 4.5 mL, 9 mmol) was added. The
contents were stirred at room temperature for 14 hours. Water was
added and THF was removed in vacuo. The solution was extracted with
EtOAc (2.times.20 mL) and the combined organic extracts were dried
over sodium sulfate and concentrated in vacuo. Purification by
flash chromatography eluting with hexanes/EtOAc (1:1) afforded
N,N-diethyl-2,5-dimethylnicotinamide as an oil (0.31 g).
[0108] The above diethylamide was reacted with
4-diethylaminobenzonitrile under conditions described in Method A
(Example 1) and the resulting intermediate reacted as described in
Methods B and C (Examples 2 and 3) to provide the title compound.
.sup.1H NMR (CDCl.sub.3, 400 MHz) .delta.8.85 (d, 1H), 7.29 (d,
1H), 3.65 (b, 2H), 3.13 (q, 2H), 2.47 (s, 3H), 2.31 (s, 3H), 1.27
(t, 3H), 1.05 (t, 3H); MS (m/e) calculated for
C.sub.12H.sub.18N.sub.2O 206, found 207 (M+H).
EXAMPLE 10
[0109]
5-(3Aminopropylamino-7-(3'-fluorobiphenyl-3-yl)-[1,6]-naphthyridine
40
[0110] This example is representative of a procedure using a Suzuki
reaction carried out on a resin-bound intermediate. This procedure
may be used to prepare compounds with a substituted biphenyl in the
7-position.
[0111] A suspension of
5-(3-aminopropylamino)-7-(3-bromophenyl)-6H-[1,6]na- phthyridine
(250 mg, 0.69 mmol) and p-nitrophenylcarbamate Wang resin (500 mg,
0.93 mmol/g loading, 0.47 mmol) in DMF (5 mL) was stirred overnight
at room temperature. The suspension was filtered and the resin was
washed successively three times with DMF, MeOH, and
dichloromethane, then dried under vacuum overnight to afford resin
bound compound.
[0112] The resin-bound compound above (50 mg, 0.0465 mmol),
dichloro(bis-triphenylphosphine) palladium (3 mg, 0.0042 mmol),
potassium carbonate (50 .mu.l of a 400 mg/mL solution in water) and
3-fluorophenylboronic acid (64 mg, 0.232 mmol) were suspended in a
7:3:2 mixture of dimethoxyethane/water/EtOH and allowed to heat to
140.degree. C. for 30 minutes in a Smith Synthesizer microwave
apparatus from Personal Chemistry. The reaction was filtered and
washed three times successively with DMF, MeOH, and
dichloromethane. The resin was then dried overnight under vacuum
and product was cleaved from the resin using 50% trifluoroacetic
acid/dichloromethane (1 mL). The resin was filtered, washed once
with 50% trifluoroacetic acid/dichloromethane (1 mL), and the
filtrate concentrated to dryness in vacuo to afford the title
compound as a film (8 mg). MS (m/e) calculated for
C.sub.23H.sub.21FN.sub.4 372.17, found 373.2 (M+H).
EXAMPLE 11
[0113]
5-(4-Aminobutoxy)-7-(4-dimethylarninophenyl)-[1,6]naphthyridine
(Method D)
[0114] This example is illustrative of a procedure that may be used
to prepare compounds with a substituted alkoxy in the 5-position.
41
[0115] To a solution of 4-amino-1-butanol (100 .mu.L, 1 mmol) in
DMF (500 .mu.L) was added sodium hydride (60% suspension in oil, 5
mg, 0.1 mmol), and the contents stirred at room temperature for 20
minutes . 5-Chloro-7-(4-dimethylaminophenyl)-[1,6]naphthyridine
(Example 2) (15 mg, 50 .mu.mol) was added, and the contents stirred
at room temperature for 2 hours. Water (200 .mu.L) was added to
quench the reaction. Purification by preparative TLC plate
developed using 10% 2 M ammonia-MeOH in dichloromethane afforded
the title compound as a yellow solid (3 mg). MS calculated for
C.sub.20H.sub.24N.sub.4O 336, found (M+H) 337 (100%).
EXAMPLE 12
[0116] 7-(4-Dimethylaminophenyl)-8-iodo-[1,6]naphthyridin-5-ol
42
[0117] The following example illustrates how an iodo substituent
may be introduced in the 8-position. Further synthetic modification
of the iodo group by methods known to those skilled in the art may
be carried out to obtain other desired substituents in the
8-position (see for instance, Example 13).
[0118] A solution of
7-(4-dimethylaminophenyl)-[1,6]naphthyridin-5-ol (Example 1) (293
mg, 1.10 mmol) and N-iodosuccinimide (261 mg, 1.16 mmol) in DMF (7
mL) was stirred in the dark at room temperature for 4 hours. The
solution was poured into dichloromethane (50 mL), washed with
aqueous sodium bicarbonate (2.times.20 mL), dried over anhydrous
magnesium sulfate and concentrated in vacuo to afford the title
compound as a yellow solid (390 mg). .sup.1H NMR (CD.sub.3OD, 400
MHz) .delta.8.95 (dd, 1H), 8.60 (dd, 1H), 7.53 (dd, 1H), 7.40 (d,
2H), 6.84 (d, 2H), 2.86 (s, 6H).
EXAMPLE 13
[0119] 8-Cyano-7-(4-dimethylaminophenyl)-[1,6]naphthyridin-5-ol
43
[0120] A solution of
7-(4-dimethylaminophenyl)-8-iodo-[1,6]naphthyridin-5-- ol (Example
12) (134 mg, 0.34 mmol), cuprous cyanide (123 mg, 1.37 mmol),
tris(dibenzylideneacetone)dipalladium (13 mg, 0.0136 mmol) and
1,1'-bis(diphenylphosphino)-ferrocene (30 mg, 0.054 mmol) in
1,4-dioxane (4 mL) was heated to 100.degree. C. for 16 hours. The
dark solution was allowed to cool to room temperature and EtOAc (10
mL) was added. This solution was poured through an extraction tube
filled with diatomaceous earth loaded with saturated sodium
bicarbonate solution, and a further 20 mL EtOAc was added to wash
the tube. The combined organic extracts were concentrated in vacuo.
Purification by flash chromatography eluting with 2.5%
MeOH/dichloromethane afforded the title compound as a yellow solid
(24 mg). .sup.1H NMR (DMSO-d6, 400 MHz) .delta.9.01 (dd, 1H), 8.52
(m, 1H), 7.64 (d, 2H), 7.59 (dd, 1H), 6.85 (d, 2H), 3.03 (s, 6H),
MS (m/e) calculated for C17H14N4O 290, found (M+H) 291 (100%).
[0121] The cyano group may be modified by methods known in the art
to obtain additional substituents in the 8-position. Intermediates
may be converted to compounds of formula (I) by Methods B and
C.
EXAMPLE 14
[0122] 7-(4-Methoxyphenyl)-[1,6]naphthyridin-5-ol (Method E) 44
[0123] 1-(4-Methoxyphenyl)acetylacetone (81 g, 0.5 mol) was added
to a solution of NaOEt in EtOH (18.7 g Na in 400 mL abs. EtOH) at
room temperature. Then 2-chloronicotinic acid (53.5 g) and CuOAc
(2.7 g) in 100 mL of EtOH was added and the reaction was refluxed
for 4 hours. The cooled solution was acidified with HOAc (330 mL).
The material was purified by pouring into CHCl.sub.3/MeOH,
filtering and evaporating. The resultant solid was then washed with
benzene to give 61.7 g (67%)
2-[2-(4-methoxyphenyl)-2-oxo-ethyl]-nicotinic acid, mp
158-163.degree. C.
[0124] The above nicotinic acid derivative (20 g) was combined with
dioxane (100 mL) and conc. NH.sub.3 (200 mL) and heated to reflux.
The product was washed with H.sub.2O and yielded 13.2 g (71%) of
the title compound as a beige solid, mp 295-298.degree. C.
EXAMPLE 15
[0125] 5-Chloro-7-(4-methoxyphenyl)-[1,6]naphthyridine (Method B)
45
[0126] 7-(4-Methoxyphenyl)-[1,6]naphthyridin-5-ol (13 g) and
POCl.sub.3 (275 mL) was heated to 135.degree. C. for 26 hours in a
sealed tube. The mixture was cooled and the POCl.sub.3 was
evaporated. The residue was poured onto ice and neutralized with 2M
Na.sub.2CO.sub.3 and the precipitate was filtered and washed with
H.sub.2O to yield 13.8 g of the title compound (98.5%), mp
126-127.degree. C.
EXAMPLE 16
[0127] 5-(3-Aminoethylamino)-7-(4-methoxyphenyl)-[1,6]naphthyridine
46
[0128] 5-Chloro-7-(4-methoxyphenyl)-[1,6]naphthyridine (50 mg) was
dissolved in ethylenediamine (1 mL) and heated to 140.degree. C.
for 16 hours. The mixture was concentrated and azeotroped with
n-BuOH. The residue was treated with 1N NaOH (10 mL) and extracted
with dichloromethane (2.times.50 mL). The product was purified by
column chromatography (dichloromethane/MeOH 0 to 10%) to yield the
title compound (20 mg, 92%), mp 134-136.degree. C.
EXAMPLE 17
[0129] 7-(4-Methoxyphenyl)-[1,6]naphthyridin-5-yl]-methyl-amine
(Method C) 47
[0130] 5-Chloro-7-(4-methoxyphenyl)-[1,6]naphthyridine (75 mg) was
suspended in aqueous methylamine (0.8 mL) and heated to 150.degree.
C. for 18 hours. The mixture was concentrated and azeotroped with
EtOH (2.times.25 mL). The residue was purified by column
chromatography (dichloromethane/5% MeOH) and finally recrystallized
from ether to yield the title compound (39 mg, 53%), mp
183-185.degree. C.
EXAMPLE 18
[0131] 7-Phenyl-8-cyano-6H-[1,6]naphthyridin-5-one (Method E)
48
[0132] NaH (36 mg 1.5 mmol) was added in portions to anhydrous EtOH
(4 mL) under N.sub.2. After 30 minutes, benzoylacetonitrile (218
mg, 1.5 mmol) was added, and the reaction mixture was allowed to
stir under a nitrogen atmosphere for 30 minutes.
2-Chloronicotinamide (236 mg, 1.5 mmol) and CuOAc (36 mg, 0.3 mmol)
were then added and the reaction mixture was heated to 50.degree.
C. The temperature was maintained for 10 days, after which time the
reaction mixture was allowed to cool, was diluted with a 10%
aqueous solution of NH.sub.4Cl (25 mL), and extracted with three
portions of EtOAc (30 mL). The combined extracts were washed with
brine, dried and evaporated to an oil which was triturated with
CH.sub.2Cl.sub.2. The resulting solid (39 mg) was recrystallized
from EtOH providing 30 mg (7.3%) of the title compound, mp
292-5.degree. C.
EXAMPLE 19
[0133] 7-(4-Methoxyphenyl)-2-trifluoromethyl-[1,6]naphthyridin-5-ol
(Method A) 49
[0134] A solution of LDA (2.4 mmol) was prepared by adding n-BuLi
(1.7 mL, 2.4 mmol, 1.4 M in hexanes) via syringe to a solution of
diisopropylamine (247 mg, 2.4 mmol) in THF (10 mL) at 0.degree. C.
and stirring for 15 minutes. The solution was then cooled to
-40.degree. C., then 2-methyl-6-trifluoromethylnicotinic acid (250
mg, 1.2 mmol) in THF (5 mL) was added via syringe. After stirring
at -40.degree. C. for 30 minutes, 4-methoxybenzonitrile (162 mg,
1.2 mmol) in THF (5 mL) was added via syringe. The reaction mixture
was allowed to warm to room temperature and stirred for 16 hours.
The reaction was quenched by addition of sat. NH.sub.4Cl solution.
A precipitate formed which was collected by vacuum filtration
giving the title compond (150 mg, 40%) as an off white solid.
EXAMPLE 20
[0135]
5-Chloro-7-(4-methoxyphenyl)-2-trifluoromethyl-[1,6]naphthyridine
(Method B) 50
[0136] POCl.sub.3 (3 mL) was added to
7-(4-methoxyphenyl)-2-trifluoromethy- l-[1,6]naphthyridin-5-ol
(Example 19) (125 mg, 0.4 mmol). The mixture was heated at
100.degree. C. for 3 hours. The excess POCl.sub.3 was concentrated
under reduced pressure. The residue was diluted with
CH.sub.2Cl.sub.2, washed with sat. NaHCO.sub.3, dried over
Na.sub.2SO.sub.4, and concentrated giving the title compound (73
mg, 55%) as an orange solid.
EXAMPLE 21
[0137]
5-(3-Aminopropylamino)-2-trifluoromethyl-7-(4-methoxyphenyl)-[1,6]n-
aphthyridine (Method C) 51
[0138] A mixture of
5-chloro-7-(4-methoxy-phenyl)-2-trifluoromethyl-[1,6]n-
aphthyridine (55 mg, 0.2 mmol) and 1,3-diaminopropane (2.5 mL) in a
sealed reaction vessel was heated at 100.degree. C. for 3 hours.
The mixture was diluted with toluene and concentrated three times
to azeotropically remove excess 1,3-diaminopropane. The residue was
diluted with EtOAc, washed sequentially with sat. NaHCO.sub.3,
H.sub.2O, sat. NaCl, dried over Na.sub.2SO.sub.4, and concentrated
giving the title compound (60 mg, quantitative) as a yellow
solid.
EXAMPLE 22
[0139] 7-(4-Methoxyphenyl)-3-bromo -[1,6]naphthyridin-5-ol (Method
E) 52
[0140] A solution of NaOEt (0.6 mmol) was prepared by adding Na (15
mg, 0.6 mmol) to EtOH (5 mL) and stirring at room temperature for
30 minutes. 1-(4-Methoxyphenyl)-butane-1,3-dione (121 mg, 0.6 mmol)
was added and the mixture was stirred at room temperature for 15
minutes. Then 5-bromo-2-chloronicotinic acid (100 mg, 0.4 mmol) and
CuOAc (10 mg, catalytic) was added. The mixture was then heated to
reflux for 4 hours. The mixture was cooled to room temperature and
quenched by addition of HOAc (0.5 mL). The mixture was
concentrated, then diluted with EtOAc and washed with 1M NaOH
(.times.2). The basic washings were acidified with 6N HCl
(pH.about.3). A precipitate formed and was collected by vacuum
filtration giving after air drying
5-bromo-2-[2-(4-methoxyphenyl)-2-oxo-e- thyl]-nicotinic acid (65
mg, 44%) as a light brown solid.
[0141] The above nicotinic acid derivative (310 mg, 0.5 mmol) in
ethanolic ammonia (2 mL, 0.2M) was heated at 90.degree. C. in a
sealed reaction vessel for 20 hours. A precipitate formed upon
cooling, and was collected by vacuum filtration giving the title
compound (120 mg, 73%) as an off white solid.
EXAMPLE 23
[0142]
5-(3-Aminopropylamino)-3-bromo-7-(4-methoxyphenyl)-[1,6]naphthyridi-
ne (Methods B and C) 53
[0143] POCl.sub.3 (3 mL) was added to
7-(4-methoxyphenyl)-3-bromo-[1,6]nap- hthyridin-5-ol (Example 22)
(115 mg, 0.4 mmol). The mixture was heated at 100.degree. C. for 3
hours. The excess POCl.sub.3 was concentrated under reduced
pressure. The residue was diluted with CH.sub.2Cl.sub.2, washed
with sat. NaHCO.sub.3, dried over Na.sub.2SO.sub.4, and
concentrated giving
5-chloro-7-(4-methoxyphenyl)-3-bromo-[1,6]naphthyridine (115 mg,
94%) as a yellow solid.
[0144] A mixture of the above intermediate (98 mg, 0.3 mmol) and
1,3-diaminopropane (3 mL) in a sealed reaction vessel was heated at
100.degree. C. for 3 hours. The mixture was diluted with toluene
and concentrated three times to azeotropically remove excess
1,3-diaminopropane. The residue was triturated with 25%
MeOH/Et.sub.2O giving the title compound (7 mg, 7%) as an orange
solid.
EXAMPLE 24
[0145]
5-(3-Aminopropylamino)-7-(4-methoxyphenyl)-3-phenyl-[1,6]naphthyrid-
ine 54
[0146] Argon gas was bubbled through a mixture of the product of
Example 23 (68 mg, 0.2 mmol), PhB(OH).sub.2 (33 mg, 0.3 mmol), and
Pd(PPh.sub.3).sub.4 (25 mg, 0.02 mmol) in 2M Na.sub.2CO.sub.3 (1
mL/EtOH (1 mL)/benzene (3 mL) for 10 minutes. The mixture was then
heated to reflux for 20 hours. After cooling to room temperature
the mixture was partitioned between EtOAc and H.sub.2O. The organic
material was washed with sat. NaCl, dried over Na.sub.2SO.sub.4,
and concentrated. The crude residue was fractionated by column
chromatography on silica gel (50% EtOAc/45% EtOH/5% NH.sub.4OH)
giving the title compound (10 mg, 15%) as a light yellow solid.
EXAMPLE 25
[0147] 2-Chloro-7-(4-methoxy-phenyl)-[1,6]naphthyridin-5-1 (Method
F) 55
[0148] Elemental sodium (173 mg, 7.5 mmol) was added to 40 mL of
absolute EtOH stirring under nitrogen. After all of the sodium
reacted, 4-methoxyacetophenone (1.44 g, 7.5 mmol) was added, and
the reaction stirred an additional 15 minutes.
2,6-Dichloronicotinic acid (1 g, 5.2 mmol) and copper acetate (80
mg, 0.65 mmol) was added and the reaction was stirred at reflux for
16 hours. The reaction was cooled to room temperature and quenched
with 1 mL concentrated acetic acid. The mixture was concentrated to
a solid, and redissolved in EtOAc. A small portion of fine
inorganic precipitate was filtered off. The organic layer was
extracted with 3.times.100 mL 1N NaOH. This was acidified with 1 N
HCl to a pH of .about.4. A precipitate formed and was collected by
vacuum filtration, yielding
6-chloro-2-[2-(4-methoxyphenyl)-2-oxo-ethyl]-nicotin- ic acid (1 g,
63%) as a tan solid.
[0149] The above nicotinic acid derivative (1 g, 3.27 mmol) was
dissolved in 50% aqueous sulfuric acid v/v (10 mL) and stirred at
room temperature for 20 hours. The reaction started as a yellow
solution, and a precipitate formed after an hour. The reaction was
added to ice water and a fine yellow precipitate formed. This was
collected by vacuum filtration, yielding
2-chloro-7-(4-methoxyphenyl)-pyrano[4,3-b]pyridin-5-- one (876 mg,
93%) as a light yellow solid.
[0150] The above intermediate (225 mg, 0.78 mmol) and 2M ammonia in
EtOH (15 mL) were combined and heated to 80.degree. C. for 16
hours. A heavy white precipitate formed. Vacuum filtration yielded
the title compound (164 mg, 73%) as a white solid.
EXAMPLE 26
[0151]
2,5-Bis-(Aminopropylamino)-7-(4-methoxyphenyl)-[1,6]naphthyridine
56
[0152] 2-Chloro-7-(4-methoxyphenyl)-[1,6]naphthyridin-5-ol (135 mg,
0.47 mmol) was dissolved in phosphorus oxychloride (2 mL, 21.5
mmol) and refluxed for 3 hours. An orange solid precipitated. The
reaction mixture was diluted with dichloromethane (25 mL) and
concentrated to an orange solid. The solid was re-dissolved in
EtOAc (300 mL) and washed with saturated sodium bicarbonate
solution (100 mL), brine (100 mL) and dried over magnesium sulfate.
Filtration and concentration yielded
2,5-dichloro-7-(4-methoxy-phenyl)-[1,6]naphthyridine (138 mg, 86%)
as a yellow solid.
[0153] The above intermediate (135 mg, 0.44 mmol),
(3-aminopropyl)-carbami- c acid tert-butyl ester (0.31 mL, 1.77
mmol), and diethyl propylamine (0.15 mL, 0.88 mmol) were combined
in methoxyethanol (5 mL) and stirred at 120.degree. C. for 16
hours. Column chromatography afforded
{3-[5-chloro-7-(4-methoxyphenyl)-[1,6]naphthyridin-2-ylamino]-propyl}-car-
baric acid tert-butyl ester (148 mg, 76%).
[0154] A mixture of the above intermediate (50 mg, 0.113 mmol) and
1,3-diaminopropane (1 mL, 12 mmol) in a sealed reaction vessel was
heated at 115.degree. C. for 3 hours. The mixture was diluted with
toluene and concentrated three times to azeotropically remove
excess 1,3-diaminopropane. The residue was triturated to a solid
with ether and filtered. The product was purified on a preparatory
TLC plate, providing
{3-[5-(3-aminopropylamino)-7-(4-methoxyphenyl)-[1,6]naphthyridin-2-ylamin-
o]-propyl}-carbamic acid tert-butyl ester (53 mg, 99%).
[0155] The above carbamic acid tert-butyl ester (50 mg, 0.104 mmol)
was stirred in 4N HCl in dioxane (2 mL) for 4 hours. A fine
precipitate formed which was filtered and re-dissolved in slightly
acidic MeOH. Solid phase extraction on a Varian SCX cartridge (2 g)
afforded the title compound (4 mg, 8%).
EXAMPLE 27
[0156]
5-(3-Aminopropylamino)-2-benzyloxy-7-(4-methoxyphenyl)-[1,6]naphthy-
ridine 57
[0157] 2,5-Dichloro-7-(4-methoxyphenyl)-[1,6]naphthyridine (Example
26) (700 mg, 2.29 mmol) was stirred in saturated sodium bicarbonate
solution (100 mL) for 20 minutes. A white solid precipitated and
was isolated by vacuum filtration to afford
5-chloro-7-(4-methoxy-phenyl)-1H-[1,6]naphthy- ridin-2-one (209 mg,
32%).
[0158] The above intermediate (100 mg, 0.35 mmol), benzyl bromide
(0.05 mL, 0.42 mmol), and silver carbonate (115 mg, 0.42 mmol) were
combined in THF (5 mL) and stirred at room temperature for 72
hours. The reaction mixture was filtered to remove inorganic salts,
and concentrated to an oil. Column chromatography afforded
2-benzyloxy-5-chloro-7-(4-methoxyphen- yl)-[1,6]naphthyridine (61
mg, 46%).
[0159] A mixture of the above intermediate (61 mg, 0.162 mmol),
1,3-diaminopropane (2 mL, 24 mmol), and methoxyethanol (2 mL) in a
sealed reaction vessel was heated at 120.degree. C. for 24 hours.
The mixture was diluted with toluene and concentrated three times
to azeotropically remove excess 1,3-diaminopropane. Column
chromatography yielded the title compound (45 mg, 67%).
EXAMPLE 28
[0160]
5-(3-Aminopropylamino)-2-benzylamino-7-(4-methoxyphenyl)-[1,6]napht-
hyridine 58
[0161] 2,5-Dichloro-7-(4-methoxyphenyl)-[1,6]naphthyridine (147 mg,
0.48 mmol) and benzyl amine (0.055 mL, 0.5 mmol) were combined in
methoxyethanol (2 mL) and stirred at 80.degree. C. for 72 hours.
The crude reaction mixture was concentrated, and column
chromatography afforded
benzyl-[5-chloro-7-(4-methoxyphenyl)-[1,6]naphthyridin-2-yl]-ami-
ne (55 mg, 30%).
[0162] A mixture of the above intermediate (50 mg, 0.133 mmol),
1,3-diaminopropane (2 mL, 24 mmol), and methoxyethanol (2 mL) in a
sealed reaction vessel was heated at 120.degree. C. for 24 hours.
The mixture was diluted with toluene and concentrated three times
to azeotropically remove excess 1,3-diaminopropane. Preparatory TLC
afforded the title compound (46 mg, 83%).
[0163] Assessment of Biological Properties
[0164] The inhibition of SYK kinase was measured with the following
assay.
[0165] SYK was purified as a GST-fusion protein. The kinase
activity was measured using DELFIA (Dissociation Enhanced
Lanthanide Fluoroimmunoassay) which utilizes europium
chelate-labeled anti-phosphotyrosine antibodies to detect phosphate
transfer to a random polymer, poly Glu.sub.4: Tyr.sub.1
(PGTYR).
[0166] The kinase assay was performed in kinase assay buffer (50 mM
HEPES, pH 7.0, 25 mM MgCl.sub.2, 5 mM MnCl.sub.2, 50 mM KCl, 100
.mu.M Na.sub.3VO.sub.4, 0.2% BSA, 0.01% CHAPS). Test samples
initially dissolved in DMSO at 1 mg/mL, were pre-diluted for dose
response (11 doses with starting final concentration of 30
.mu.g/mL, 1 to 3.5 serial dilutions) with the assay buffer in
96-well polypropylene microtiter plates. A 25 .mu.L aliquot of this
diluted sample was added to neutravidin coated 96-well white plate
(PIERCE). A 25 .mu.L volume of diluted enzyme (0.6 ng/mL final
conc.) and a 50 .mu.L volume of a mixture of substrates containing
200 nM ATP and 3.6 ng/.mu.L PGTYR-biotin (CIS Biointemational) in
kinase buffer was sequentially added to the assay plates.
Background wells were incubated with buffer, rather than 25 .mu.L
enzyme. The assay plates were incubated for 30 minutes at room
temperature. Following incubation, the assay plates were washed
three times with 300 .mu.L wash buffer (50 mM Tris-HCL, pH 7.4, 150
mM NaCl, 0.05% Tween 20, 0.2% BSA). A 100 .mu.L aliquot of
europium-labeled anti-phosphotyrosine (Eu.sup.3+-PT66, Wallac
CR04-100) diluted in 50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 10 .mu.M
DTPA, 0.05% Tween 40, 0.2% BSA, 0.05% BGG (1 nM final conc.) was
added to each well and incubated for 30 minutes at room
temperature. Upon completion of the incubation, the plate was
washed four times with 300 .mu.L of wash buffer and 100 .mu.L of
DELFIA Enhancement Solution (Wallac) was added to each well. After
10 minutes or longer, time-resolved fluorescence was measured on
the LJL's Analyst (excitation at 360 nm, emission at 620 nm, EU 400
Dichroic Mirror) after a delay time of 250 .mu.s.
[0167] Compounds of the invention including those in the Synthetic
Examples section and those listed individually in the Detailed
Description of the Invention section were evaluated for inhibition
of SYK kinase in this assay. All had IC.sub.50s below 30 .mu.M,
preferred compounds had IC.sub.50s below 1 .mu.M.
[0168] Methods of Therapeutic Use
[0169] The compounds of the invention are useful in inhibiting the
activity of SYK kinase. In doing so, these compounds are useful in
blocking disease processes mediated by SYK kinase. Compounds of the
invention effectively block activation of mast cells by inhibiting
SYK kinase. This in turn prevents the release of inflammatory
mediators including histamine, proteases, leukotrienes and
cytokines. These mediators play a key role in the etiology of
allergic and inflammatory disorders. Preventing the release of
these mediators is a desirable means for treating these conditions.
Thus there are provided methods for treating these conditions using
the compounds of the invention. These include inflammatory and
allergic conditions involving mast cell activation, including but
not limited to asthma, chronic obstructive pulmonary disease
(COPD), adult respiratory distress syndrome (ARDS), ulcerative
colitis, Crohn's disease bronchitis, conjunctivitis, psoriasis,
scleroderma, urticaria, dermatitis and allergic rhinitis. They also
include inflammatory conditions where B cells contribute to the
etiology of the disease, including but not limited to lupus and
rheumatoid arthritis. The compounds of the invention can also be
used to treat other disorders associated with inappropriate mast
cell or B cell activation, unrelated to those listed above or
discussed in the Background of the Invention. Therefore, the
invention also provides methods of treating allergic and
inflammatory diseases comprising administering to a patient in need
of such treatment a pharmaceutically effect amount of a compound
according to the invention.
[0170] For therapeutic use, the compounds of the invention may be
administered in any conventional dosage form in any conventional
manner. Routes of administration include, but are not limited to,
intravenously, intramuscularly, subcutaneously, intrasynovially, by
infusion, sublingually, transdermally, orally, topically or by
inhalation. The preferred modes of administration are oral and
intravenous. Compositions comprising the compounds of the invention
for each of the aforementioned routes of administration will be
apparent to the skilled artisan. The invention also provides for
pharmaceutical compositions including a therapeutically effective
amount of the compounds according to the invention. Such
pharmaceutical compositions will include pharmaceutically
acceptable carriers and adjuvants as further described below.
[0171] The compounds of this invention may be administered alone or
in combination with adjuvants that enhance stability of the
inhibitors, facilitate administration of pharmaceutical
compositions containing them in certain embodiments, provide
increased dissolution or dispersion, increase inhibitory activity,
provide adjunct therapy, and the like, including other active
ingredients. Advantageously, such combination therapies utilize
lower dosages of the conventional therapeutics, thus avoiding
possible toxicity and adverse side effects incurred when those
agents are used as monotherapies. Compounds of the invention may be
physically combined with the conventional therapeutics or other
adjuvants into a single pharmaceutical composition. Advantageously,
the compounds may then be administered together in a single dosage
form. In some embodiments, the pharmaceutical compositions
comprising such combinations of compounds contain at least about
15%, but more preferably at least about 20%, of a compound of the
invention (w/w) or a combination thereof. Alternatively, the
compounds may be administered separately (either serially or in
parallel). Separate dosing allows for greater flexibility in the
dosing regime.
[0172] As mentioned above, dosage forms of the compounds of this
invention include pharmaceutically acceptable carriers and
adjuvants known to those of ordinary skill in the art. These
carriers and adjuvants include, for example, ion exchangers,
alumina, aluminum stearate, lecithin, serum proteins, buffer
substances, water, salts or electrolytes and cellulose-based
substances. Preferred dosage forms include, tablet, capsule,
caplet, liquid, solution, suspension, emulsion, lozenges, syrup,
reconstitutable powder, granule, suppository and transdermal patch.
Methods for preparing such dosage forms are known (see, for
example, H. C. Ansel and N. G. Popovish, Pharmaceutical Dosage
Forms and Drug Delivery Systems, 5th ed., Lea and Febiger (1990)).
Dosage levels and requirements are well-recognized in the art and
may be selected by those of ordinary skill in the art from
available methods and techniques suitable for a particular patient.
In some embodiments, dosage levels range from about 10-1000 mg/dose
for a 70 kg patient. Although one dose per day may be sufficient,
up to 5 doses per day may be given. For oral doses, up to 2000
mg/day may be required. As the skilled artisan will appreciate,
lower or higher doses may be required depending on particular
factors. For instance, specific dosage and treatment regimens will
depend on factors such as the patient's general health profile, the
severity and course of the patient's disorder or disposition
thereto, and the judgment of the treating physician.
* * * * *