U.S. patent application number 10/193137 was filed with the patent office on 2003-07-10 for inhibitors of alpha 4beta1 mediated cell adhesion.
This patent application is currently assigned to Tanabe Seiyaku Co., Ltd.. Invention is credited to Chrusciel, Robert A., Fisher, Jed F., Lobl, Thomas J., Polinsky, Alexander, Rishton, Gilbert M., Tanis, Steven, Teegarden, Bradley R., Thomas, Edward W., Yamagishi, Masafumi.
Application Number | 20030130349 10/193137 |
Document ID | / |
Family ID | 21965686 |
Filed Date | 2003-07-10 |
United States Patent
Application |
20030130349 |
Kind Code |
A1 |
Lobl, Thomas J. ; et
al. |
July 10, 2003 |
Inhibitors of alpha 4beta1 mediated cell adhesion
Abstract
The present invention relates to small molecules according to
the formula [I]: 1 which are potent inhibitors of
.alpha..sub.4.beta..sub.1 mediated adhesion to either VCAM or CS-1
and which can be used for treating or preventing
.alpha..sub.4.beta..sub.1 adhesion mediated conditions in a mammal
such as a human.
Inventors: |
Lobl, Thomas J.; (Foster
City, CA) ; Teegarden, Bradley R.; (San Diego,
CA) ; Polinsky, Alexander; (San Diego, CA) ;
Rishton, Gilbert M.; (Thousand Oaks, CA) ; Yamagishi,
Masafumi; (Hyogo-ken, JP) ; Tanis, Steven;
(Kalamazoo, MI) ; Fisher, Jed F.; (Three Rivers,
MI) ; Thomas, Edward W.; (Kalamazoo, MI) ;
Chrusciel, Robert A.; (Portage, MI) |
Correspondence
Address: |
BIRCH STEWART KOLASCH & BIRCH
PO BOX 747
FALLS CHURCH
VA
22040-0747
US
|
Assignee: |
Tanabe Seiyaku Co., Ltd.
|
Family ID: |
21965686 |
Appl. No.: |
10/193137 |
Filed: |
July 12, 2002 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10193137 |
Jul 12, 2002 |
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09102584 |
Jun 23, 1998 |
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60050515 |
Jun 23, 1997 |
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Current U.S.
Class: |
514/563 ;
514/519; 514/616; 558/432; 562/503; 564/152 |
Current CPC
Class: |
C07D 295/192 20130101;
C07C 233/83 20130101; C07C 235/84 20130101; C07C 271/22 20130101;
C07D 233/64 20130101; C07D 295/185 20130101; C07C 271/28 20130101;
C07D 213/81 20130101; C07C 235/40 20130101; C07D 207/16 20130101;
C07C 233/60 20130101; C07C 275/26 20130101; C07C 237/40 20130101;
C07C 275/42 20130101; C07C 237/42 20130101; C07C 255/29 20130101;
C07C 235/64 20130101; C07D 213/64 20130101; C07C 233/63 20130101;
C07D 217/26 20130101; C07D 307/68 20130101; C07C 237/22 20130101;
C07D 213/82 20130101; C07C 255/46 20130101; C07C 259/08 20130101;
C07D 333/24 20130101; C07C 311/21 20130101; C07C 229/46 20130101;
C07D 209/20 20130101; C07C 233/87 20130101; C07C 311/64
20130101 |
Class at
Publication: |
514/563 ;
514/616; 562/503; 564/152; 558/432; 514/519 |
International
Class: |
A61K 031/275; A61K
031/195; A61K 031/16 |
Claims
What is claimed is:
1. A compound of the formula (I): 401wherein n is an integer of 0
or 1; R.sup.1 is a hydrogen atom or methyl group, R.sup.2 is a
group of the formula: --CN, --COOH, --(C.sub.1-6 alkylene)OH,
--CH.sub.2O(C.sub.1-6 alkyl), --(C.sub.1-6 alkylene)COOH,
--CH.sub.2O(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl)
--CH.sub.2O(C.sub.1-6 alkylene)COOH, --(C.sub.2-7 alkenylene)COOH,
--CO(C.sub.1-6 alkylene)COOH, --CO(C.sub.2-7 alkenylene)COOH,
--CO(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl) --CO(C.sub.1-6
alkylene)CO(C.sub.1-6 alkyl), --CONH(C.sub.1-6 alkyl),
--CONHO(C.sub.1-6 alkyl), --CONH(C.sub.1-6 alkylene)COOH,
--CONH.sub.2, --CONH(C.sub.3-7 cycloalkyl), 402--CONHOCH.sub.2Ph,
--CONH(C.sub.1-6 alkylene)CN, --COO(C.sub.1-6 alkyl),
--CH.sub.2O(C.sub.1-6 alkylene)CONH.sub.2, --CONH(C.sub.1-6
alkylene)CONH.sub.2, --CONHOH, --NHCOOCH.sub.2Ph, 403R.sup.3 is a
hydrogen atom or a methyl group; X is a methylene group or a group
of the formula: --CO--, R.sup.4 is a hydrogen atom or a C.sub.1-6
alkyl group; R.sup.5 is a group of the formula: --COOH or an ester
or an amide thereof, --(C.sub.1-6 alkylene)COOH or an ester or an
amide thereof, --(C.sub.1-7 alkylene)O(C.sub.1-6 alkyl),
--(C.sub.1-7 alkylene)OH, --COO(C.sub.1-6 alkyl), --CONH(C.sub.1-6
alkyl), or --CONH.sub.2; R.sup.6 is a substituent of the formula:
404wherein, R.sup.7, which occurs one or more times and which may
be the same or different in each occurrence, is --H, --OH,
--NO.sub.2, --NH.sub.2, --C.sub.1-C.sub.5 alkyl, --F, --Cl, --Br,
--I, --COOH, --COO(C.sub.1-6 alkyl), --O(C.sub.1-C.sub.8 alkyl),
--CONH(C.sub.1-6 alkylene)COOH, --OCH.sub.2(C.sub.3-C.sub.7
cycloalkyl) or a substituent of the formula 405R.sup.8, which
occurs one or more times and which may be the same or different in
each occurrence, is --H, --OH, --NH.sub.2, --NO.sub.2,
--C.sub.1-C.sub.7 alkyl, --F, --Cl, --Br, --I, --CF.sub.3, phenyl,
or --O(C.sub.1-6 alkyl); R.sup.9 is selected from a group of the
formula: --H, --C.sub.1-C.sub.5 alkyl, --C.sub.3-C.sub.7
cycloalkyl, --(--C.sub.1-C.sub.6 alkylene)aryl, aryl, or a
substituent of the formula: 406R.sup.10, which occurs one or more
times and which may be the same or different in each occurrence, is
--H, --F, --Cl, --Br, or --I, --NO.sub.2, --C.sub.1-6 alkyl or
--O(C.sub.1-6 alkyl); R.sup.11 is selected from a group of the
formula: 407R.sup.12, which occurs one or more times and which may
be the same or different in each occurrence, is --H, --CF.sub.3,
--OCF.sub.3, --F, --Br, --Cl, or --I; with the proviso that R.sup.1
and R.sup.3 must be different and also with the proviso that when
R.sup.2 or R.sup.6 is a moiety of the formula --COOH or contains a
moiety of the formula --COOH, then a pharmaceutically acceptable
ester or a pharmaceutically acceptable amide thereof are included,
and also with the proviso that when R.sup.7 is the formula
408R.sup.9 is other than hydrogen, and also with the proviso that
when R.sup.6 is the formula 409or a pharmaceutically acceptable
salt thereof.
2. The compound according to claim 1, which is a compound of the
formula (1-1): 410wherein n, R.sup.1 through R.sup.6 and X are as
defined above.
3. The compound according to claim 1, which is a compound of the
formula (1-2): 411wherein n, R.sup.1 through R.sup.4, R.sup.6 and X
are as defined above and R.sup.5 is a group of the formula: --COOH,
--(C.sub.1-6 alkylene)COOH, --(C.sub.1-7 alkylene)O(C.sub.1-6
alkyl) --(C.sub.1-7 alkylene)OH, --COO(C.sub.1-6
alkyl)-CONH(C.sub.1-6 alkyl), or --CONH.sub.2.
4. The compound according to claim 1, wherein R.sup.6 is 412wherein
Y is a hydrogen atom or a chlorine atom.
5. The compound according to claim 1, wherein R.sup.2 is a group of
the formula: is a group of the formula: --COOH or an ester or an
amide thereof, --CONHCH.sub.2COOH, --CONHOCH.sub.2Ph or
--CONHCH.sub.2CONH.sub.- 2.
6. The compound according to claim 1, wherein said compound is
selected from the group consisting of Examples 4, 24, 26, 28, 29,
31, 36, 38, 77, 130, 146, 194, 208, 209, 210, 212, 213, 215, and
225.
7. The compound according to claim 6, wherein said compound is
selected from the group consisting of Examples 4, 24, 26, 28, 38,
77, 130, 146, 194, 208, 209, 210, 213, 215, and 225.
8. The compound according to claim 7, wherein said compound is
selected from the group consisting of Examples 208, 209, 210, 215,
and 225.
9. The compound according to claim 1, wherein said compound is
selected from the group consisting of Examples 4, 24, 26, 28, 29,
36, 38, 77, 130, 194, 208, 209, 210, 212, 213, 215, and 225.
10. The compound according to claim 9, wherein said compound is
selected from the group consisting of Examples 4, 24, 28, 38, 77,
130, 194, 208, 209, 210, 212, 213, 215, and 225.
11. The compound according to claim 10, wherein said compound is
selected from the group consisting of Examples 209, 210, and
225.
12. The compound according to claim 1, wherein said compound is (1S
cis)-N-[3-carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-[(3,4-dichloroph-
enyl)methyl]-L-histidine.
13. A pharmaceutical composition comprising: a therapeutically
effective amount of the compound as set forth in claims 1, 2, 3, 4,
5, 6, 7, 8, 9, 10, 11, or 12; and a pharmaceutically acceptable
carrier or diluent.
14. A method for treating or preventing (4 .mu.l adhesion mediated
conditions in a human which comprises administering to a patient an
effective amount of the compound according to claim 1, 2, 3, 4, 5,
6, 7, 8, 9, 10, 11, or 12.
15. A method according to claim 14, wherein said condition is
selected from the group consisting of rheumatoid arthritis, asthma,
allergy conditions, allograft rejection, psoriasis, eczema, contact
dermatitis and other skin inflammatory diseases, inflammatory and
immunoinflammatory conditions including ophthalmic inflammatory
conditions, inflammatory bowel diseases, atherosclerosis, and
ulcerative colitis.
Description
BACKGROUND OF THE INVENTION
[0001] 1. Field of the Invention
[0002] The present invention relates to small molecules that are
potent inhibitors of .beta..sub.4.beta..sub.1 mediated adhesion to
either VCAM or CS-1 and which could be useful for the treatment of
inflammatory diseases.
[0003] 2. Description of Related Art
[0004] The extracellular matrix (ECM) is the major component of
connective tissue which provides structural integrity, and promotes
cell migration and differentiation. As part of these functions,
extracellular matrix molecules such as fibronectin, collagen,
laminin, von Willebrand factor, thrombospondin, fibrinogen, and
tenascin have been shown to support adhesion of cells in vitro.
This adhesive interaction is critical for a number of biological
processes including hemostasis, thrombosis, wound healing, tumor
metastasis, immunity and inflammation.
[0005] Fibronectin (FN) is the prototype ECM molecule. The major
cell attachment site in the fibronectin molecule has been
reproduced synthetically with the amino acid sequence
arginine-glycine-aspartic acid, or RGD using single letter
nomenclature. Peptides containing the RGD sequence which either
inhibit or promote cell adhesion have been described (U.S. Pat.
Nos. 4,589,881; 4,661,111; 4,517,686; 4,683,291; 4,578,079;
4,614,517; and 4,792,525) Changes in the peptide as small as the
exchange of alanine for glycine or glutamic acid for aspartic acid,
which constitute the addition of a single methyl or methylene group
to the tripeptide, eliminates these activities (Pierschbacher et
al., Proc. Natl. Acad. Sci. USA 81:5985 (1984)). Recently, a second
FN cell binding domain has been identified within the alternatively
spliced region of the A chain of the molecule, known as the
connecting segment 1 (CS-1). The most active cell-binding site
within this alternatively spliced region is composed of 25 amino
acids where the carboxy terminus contains the sequence EILDVPST The
amino acid sequence EILDVPST forms a recognition motif on FN for
cell surface receptors. (Wayner et al., J. Cell Biol. 109:1321
(1989); Guan et al., Cell 60:53 (1990)).
[0006] The receptors which recognize these sites on FN belong to a
gene superfamily called integrins which consist of heterodimeric
complexes of non-covalently associated alpha and beta subunits. A
common .beta. subunit combines with unique a subunits to form an
adhesion receptor of defined specificity. To date, 8 .beta.
subunits have been identified which can dimerize with 16 distinct
.alpha. subunits forming 22 distinct integrins. The .beta.1
subfamily, also known as the VLA family (Very Late Activation
Antigens), binds to ECM molecules such as FN, collagen and laminin.
For reviews, see, Hynes, Cell 48:549 (1987); Hemler, Annu. Rev.
Immunol. 8:365 (1990). Leukocyte interaction with FN at the two
spatially separate binding domains is mediated by two distinct
integrins. The RGD site is recognized by the Integrin
.alpha..sub.5.beta..sub.1, while, EILDV is recognized by
.alpha..sub.4.beta..sub.1 (Pytela et al., Cell 40:191 (1985);
Wayner et al., J. Cell Biol. 109:1321 (1989); Guan et al, Cell
60:53 (1990)).
[0007] Vascular endothelial cells form the interface between blood
and tissues and control the passage of leukocytes as well as plasma
fluid into tissues. A variety of signals generated at the site of
inflammation can activate both endothelial cells as well as
circulating leukocytes so that they become more adhesive to one
another. Following this initial adhesion the leukocytes migrate
into the tissues to perform host defense functions. Several
adhesion molecules have been identified which are involved in
leukocyte-endothelial interactions.
[0008] In the .beta..sub.1 subfamily, in addition to binding to
fibronectin, .alpha..sub.4.beta..sub.1 interacts with a cytokine
inducible protein on endothelial cells termed vascular cell
adhesion molecule (VCAM). Further involved in the
leukocyte-endothelial adhesion process is the .beta..sub.2 integrin
subfamily. .beta..sub.2 integrins include CD11a/CD18, CD11b/CD18,
and CD11c/CD18. In addition, the .beta..sub.7 subunit associates
with .alpha..sub.4 to form a unique .alpha..sub.4.beta..sub.7
heterodimer which binds to FN, to VCAM, and to Mucosal Addressin
Cell Adhesion Molecule-1 (MAdCAM) (Ruegg et al, J. Cell.Biol.
117:179 (1992); Andrew et al., J. Immunol. 153:3847 (1994); Briskin
et al., Nature 363:461 (1993); Shyjan et al, J. Immunol. 156:2851
(1996)). .alpha..sub.4 integrins are widely expressed on different
cell types including hematopoietic progenitors, lymphocytes,
natural killer cells, monocytes, eosinophils, basophils, and mast
cells (Helmer, M. E., Annu. Rev. Immunol. 8:365 (1990)). Other
molecules on endothelial cells which bind to the leukocytes include
ICAM-1, ICAM-2, E-selectin and P-selectin (Carlos and Harlan,
Immunol. Rev. 114:1 (1990); Osborn, L., Cell 62:3 (1990); Springer
T., Nature 346:425 (1990); Geng et al., Nature 347:757 (1990);
Stoolman, Cell 56:907 (1989)).
[0009] A number of in vitro and in vivo studies indicate that
.alpha..sub.4.beta..sub.1 plays a critical role in the pathogenesis
of a variety of diseases. Monoclonal antibodies directed against
.alpha..sub.4 have been tested in a variety of disease models.
Anti-.alpha..sub.4 antibodies block adhesion of lymphocytes to
synovial endothelial cells; this adhesion plays a potential role in
rheumatoid arthritis (van Dinther-Janssen et al, J. Immunol.
147:4207 (1991). .alpha..sub.4 has also been implicated with
respect to rheumatoid arthritis in separate studies (Laffon et al,
J. Clin. Invest. 88:546 (1991); Morales-Ducret et al, J. Immunol.
149:1424 (1992)). A significant number of studies have evaluated
the role of .alpha..sub.4 in allergy and asthma. For example,
monoclonal antibodies to .alpha..sub.4 block adhesion of basophils
and eosinophils to cytokine activated endothelial cells (Walsh et
al, J. Immunol. 146:3419 (1991); Bochner et al, J. Exp. Med.
173:1553 (1991)) Monoclonal antibodies to .alpha..sub.4 were also
effective in several lung antigen challenge models (Abraham et al,
J. Clin. Invest. 93:776 (1994), Weg et al, J. Exp. Med. 177:561
(1993)). The cotton-top tamarin, which experiences spontaneous
chronic colitis, showed a significant attenuation of their colitis
when anti-.alpha..sub.4 antibody was administered (Podolsky et al,
J. Clin. Invest. 92:372 (1993); Bell et al, J. Immunol. 151:4790
(1993)). In a rat and mouse model, autoimmune encephalomyelitis was
blocked by anti-.alpha..sub.4 antibody (Yednock et al, Nature
356:63 (1992); Baron et al, J. Exp. Med. 177:57 (1993))
Anti-.alpha..sub.4 monoclonal antibodies also inhibit insulitis and
delay the onset of diabetes in the non-obese diabetic mouse (Baron
et al, J. Clin. Invest. 93:1700 (1994); Yang et al, Proc. Natl.
Acad. Sci. USA 90:10494 (1993); Burkly et al, Diabetes 43:529
(1994)). .alpha..sub.4 is also implicated in atherosclerosis due to
its endothelial expression during atherogenesis (Cybuisky et al,
Science 251:788 (1991)). The migration of leukocytes to an
inflammatory site can also be blocked by anti-.alpha..sub.4
antibodies. In addition to the blocking of migration, inhibitors of
leukocyte endothelial adhesion may block the costimulatory signals
mediated by integrins and thus inhibit overproduction of
inflammatory cytokines. In a separate set of experiments not using
anti-.alpha..sub.4 antibodies, the peptides GRDGSP or EILDV were
tested against contact hypersensitivity response. The contact
hypersensitivity response was found to be blocked by GRDGSP or
EILDV suggesting that both .alpha..sub.4.beta..sub.1, and
.alpha..sub.5.beta..sub.1 are involved in this inflammatory
response.
[0010] Other ailments which may involve
.alpha..sub.4.beta..sub.1-mediated conditions include the
inflammatory disorders rheumatoid arthritis, allergic disorders,
asthma, spontaneous chronic colitis, insulitis, contact
hypersensitivity response, atherosclerosis and autoimmune
encephalomyelitis., These studies illustrate that small molecules
that are potent inhibitors of .alpha..sub.4.beta..sub.1 mediated
adhesion to either VCAM-1 or CS-1 may be used as a form of
treatment in numerous inflammatory diseases. However, these
inflammatory conditions could be expanded to include adult
respiratory distress syndrome, AIDS, cardiovascular diseases,
thrombosis or harmful platelet aggregation, reocclusion following
thrombolys's, allograft rejection, reperfusion injury, psoriasis,
eczema, contact dermatitis and other skin inflammatory diseases,
osteoporosis, osteoarthritis, atherosclerosis, neoplastic diseases
including metastasis of neoplastic or cancerous growth, wound
healing enhancement, treatment of certain eye diseases such as
detaching retina, Type I diabetes, multiple sclerosis, systemic
lupus erythematosus (SLE), inflammatory and immunoinflammatory
conditions including ophthalmic inflammatory conditions and
inflammatory bowel diseases, ulcerative colitis, regional enteritis
and other autoimmune diseases. Accordingly, a compound which could
inhibit these conditions is desirable.
SUMMARY OF THE INVENTION
[0011] The present invention is directed to a compound of the
formula [I]: 2
[0012] In the above formula [I], n is an integer of 0 or 1, R.sup.1
is a hydrogen atom or a methyl group, and R.sup.2 can be selected
from the following: a --CN group; a --COOH group; a
--(C.sub.1-6alkylene)OH group, preferably a --(C.sub.1-3alkylene)OH
group; a CH.sub.2O(C.sub.1-6 alkyl) group, preferably a
--CH.sub.2O(C.sub.1-3 alkyl) group; a --(C.sub.1-3 alkylene)COOH
group, preferably a --(C.sub.1-13 alkylene)COOH group; a
--CH.sub.2O(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl) group, preferably
a --CH.sub.2O(C.sub.1-3 alkylene)O(C.sub.6 alkyl) group or a
--CH.sub.2O(C.sub.1-6 alkylene)O(C.sub.1-3 alkyl) group, more
preferably a --CH.sub.2O(C.sub.1-3 alkylene)O(C.sub.1-3 alkyl)
group; a --CH.sub.2O(C.sub.1-6 alkylene)COOH group, preferably a
--CH.sub.2O(C.sub.1-3 alkylene)COOH group; a --(C.sub.2-7
alkenylene)COOH group, preferably a --(C.sub.2-4 alkenylene)COOH
group; a --CO(C.sub.1-6 alkylene)COOH group, preferably a
--CO(C.sub.1-3 alkylene)COOH group; a --CO(C.sub.2-7
alkenylene)COOH group, preferably a --CO(C.sub.2-4 alkenylene)COOH
group; a --CO(C.sub.1-6 alkylene)O(C.sub.6 alkyl) group, preferably
a --CO(C.sub.1-3 alkylene)O(C.sub.1-6 alkyl) group or a
--CO(C.sub.1-6 alkylene)O(C.sub.1-3 alkyl) group, more preferably a
--CO(C.sub.1-3 alkylene)O(C.sub.1-3 alkyl) group; a --CO(C.sub.1-6
alkylene)CO(C.sub.1-6 alkyl) group, preferably a --CO(C.sub.1-3
alkylene)CO(C.sub.1-6 alkyl) group or a --CO(C.sub.1-6
alkylene)CO(C.sub.1-3 alkyl) group, more preferably a
--CO(C.sub.1-3 alkylene)CO(C.sub.1-3 alkyl) group; a
--CONH(C.sub.1-6 alkyl) group, preferably a --CONH(C.sub.1-3 alkyl)
group; a --CONHO(C.sub.1-6 alkyl) group, preferably a
--CONHO(C.sub.1-3 alkyl) group; a --CONH(C.sub.1-6 alkylene) COOH
group, preferably a --CONH(C.sub.1-3 alkylene)COOH group, a
--CONH.sub.2 group; a --CONH(C.sub.3-7 cycloalkyl) group,
preferably a --CON (C.sub.3-6 cycloalkyl) group; a group as
follows: 3
[0013] a --CONHOCH.sub.2Ph group; a --CONH(C.sub.1-6 alkylene)CN
group, preferably a --CONH(C.sub.1-3 alkylene)CN group; a
--COO(C.sub.1-6 alkyl) group, preferably a --COO(C.sub.1-3 alkyl)
group; a --CH.sub.2O(C.sub.1-6 alkylene)CONH.sub.2 group,
preferably a --CH.sub.2O(C.sub.1-3 alkylene)CONH.sub.2 group; a
--CONH(C.sub.1-6 alkylene)CONH.sub.2 group, preferably a
--CONH(C.sub.1-3 alkylene)CONH.sub.2 group; a --CONHOH group; a
--NHCOOCH.sub.2Ph group; or a group selected from the following
formula: 4
[0014] In the above Formula (I), in R.sup.2, the C.sub.1-6 alkylene
is preferably C.sub.1-3 aklylene, the C.sub.2-7 alkenylene is
preferably C.sub.2-4 alkenylene, the C.sub.1-6 alkyl is preferably
C.sub.1-3 alkyl and the C.sub.3-7 cycloalkyl is preferably
C.sub.3-6 cycloalkyl.
[0015] In the above formula [I], R.sup.3 can be a hydrogen atom or
a methyl group, X can be a methylene group or a --CO-- group, and
R.sup.4 can selected from the following: a hydrogen atom; or a
C.sub.1-6 alkyl group, preferably a C.sub.1-3 alkyl group.
[0016] In the above formula [I], R.sup.5 can be a group selected
from the following: a --COOH group or an ester or an amide thereof;
a --(C.sub.1-6 alkylene)COOH group, preferably a --(C.sub.1-3
alkylene)COOH group, or an ester or an amide thereof; a
--(C.sub.1-7 alkylene)O(C.sub.1-6 alkyl) group, preferably a
--(C.sub.1-4 alkylene)O(C.sub.1-6 alkyl) group or a --(C.sub.1-7
alkylene)O(C.sub.1-3 alkyl) group, more preferably a --(C.sub.1-4
alkylene)O(C.sub.1-3 alkyl) group; a --(C.sub.1-7 alkylene)OH
group, preferably a --(C.sub.1-4 alkylene)OH group; a
--COO(C.sub.1-6 alkyl) group, preferably a --COO(C.sub.1-3 alkyl)
group; a --CONH(C.sub.1-6 alkyl) group, preferably a
--CONH(C.sub.1-3 alkyl) group; or a --CONH.sub.2 group.
[0017] In the above formula [I], R.sup.6 can be a substituted or
unsubstituted monocyclic or bicyclic aryl group, a substituted or
unsubstituted monocyclic or bicyclic heteroaryl group, a
substituted or unsubstituted monocyclic or bicyclic
arylcarbonylamino-C.sub.1-6 alkyl group, a substituted or
unsubstituted monocyclic or bicyclic aliphatic heterocyclic
carbonyl group, a 9- fluorenylmethyloxycarbonylamino --C.sub.1-6
alkyl group, a 3-tosylguanidino-C.sub.1-6 alkyl group; 5
[0018] provided that R.sup.1 and R.sup.3 must be different and when
R.sup.2 or R.sup.6 is a --COOH group or contains a --COOH group,
then a pharmaceutically acceptable ester or a pharmaceutically
acceptable amide thereof are included and also with the proviso
that [1S-[1, (R*)
3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]amino-
]-4-(2,6-dichlorobenzoyl)-.gamma.-oxo-1-pyrazinebutanoic acid
methyl ester or [1S-[1.alpha.,
(R*),3.alpha.]]-.beta.-[[(3-Carboxy-2,2,3-trimethylcycl- opentyl)
carbonyl]amino]-4-(2,6-dichlorobenzoyl)-.gamma.-oxo-1-pyrazinebut-
anoic acid methyl ester are excluded.
[0019] In the above formula [I], in R.sup.6, an aryl group or aryl
moiety in the arylcabonylamino group is a 5- or 6-membered aromatic
hydrocarbon ring; and including any bicyclic group in which any of
the above ring is fused to another above ring; and substituted by
zero (0) to three (3) substituents.
[0020] Examples of aryl can include phenyl, a C.sub.1-6
alkoxyphenyl group and naphthyl group. Each of these moieties may
be substituted as appropriate.
[0021] In R.sup.6, a heteroaryl is a 5- or 6-membered partially
saturated or unsaturated ring containing from one (1) to four (4)
heteroatoms selected from the group consisting of nitrogen, oxygen
and sulfur; and including any bicyclic group in which any of the
above heterocyclic rings is fused to a benzene ring,
C.sub.3-C.sub.8 cycloalkyl, or another heterocycle; and if
chemically feasible, the nitrogen and sulfur atoms may be in the
oxidized forms; and substituted by zero (0) to three (3)
substituents.
[0022] Examples of heteroaryl can include 2-pyridyl, 3-pyridyl,
4-pyridyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl,
3-pyridazinyl, 4-pyridazinyl, 3-pyrazinyl, 2-quinolyl, 3-quinolyl,
1-isoquinolyl, 3-isoquinolyl, 4-isoquinolyl, 2-quinazolinyl,
4-quinazolinyl, 2-quinoxalinyl, 1-phthalazinyl, 2-imidazolyl,
4-imidazolyl, 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl,
3-pyrazolyl, 4-pyrazolyl, 5-pyrazolyl, 2-oxazolyl, 4-oxazolyl,
5-oxazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 2-indolinyl,
3-indolyl, 3-indazolyl, 2-benzoazolyl, 2-benzothiazolyl,
2-benzimidazolyl, 2-benzofuryl, 3-benzofuryl, 2-furyl, 3-furyl,
2-thienyl, 3-thienyl, 2-pyrrolyl, 3-pyrrolyl,
1,2,4-oxadiazolyl-3-yl, 1,2,4-oxadiazol-5-yl,
1,2,4-thiadiazol-3-yl, 1,2,4-thiadiazol-5-yl, 1,2,4-triazol-3-yl,
1,2,4-triazol-5-yl, 1,2,3,4-tetrazol-5-yl, 5-oxazolyl, 1-pyrrolyl,
1-pyrazolyl, 1,2,3-triazol-1-yl, 1,2,4-triazol-1-yl, 1-tetrazolyl,
1-indolinyl, 1-indazolyl, 2-isoindolyl, 1-purinyl, 3-isothiazolyl,
4-isothiazolyl and 5-isothiazolyl. Each of these moieties may be
substituted as appropriate.
[0023] In R.sup.6, an aliphatic heterocyclic moiety in aliphatic
heterocyclic carbonyl group is a 5- or 6-membered saturated ring
containing from one (1) to four (4) heteroatoms selected from the
group consisting of nitrogen, oxygen and sulfur; and including any
bicyclic group in which any of the above heterocyclic rings is
fused to a benzene ring, C.sub.3-C.sub.8 cycloalkyl, or another
heterocycle; and if chemically feasible, the nitrogen and sulfur
atoms may be in the oxidized forms; and substituted by zero (0) to
three (3) substituents.
[0024] Examples of aliphatic heterocyclic can include piperazinyl
group, pyrrolidinyl group, piperidyl group, homopiperidyl group,
thiomorpholino group, and morpholino group. Each of these moieties
may be substituted as appropriate.
[0025] According to the present invention, the term "C.sub.1-6
alkyl" represents an alkyl group having 1 to 6 carbon atoms. This
group may be straight or branched. Illustrative but non-limiting
examples of a C.sub.1-6 alkyl group are methyl, ethyl, n-propyl,
isopropyl, n-butyl, iso-butyl, tert-butyl, sec-butyl, n-pentyl,
isopentyl and n-hexyl. It is understood that this type of
nomenclature extends to terms such as "C.sub.1-6 methoxy" and
therefore encompasses both straight and branced methoxy groups
having 1 to 6 carbon atoms.
[0026] Also, in the above formula [I] with all substituents as
described above, a pharmaceutically acceptable salt thereof is
included.
[0027] The desired compounds of the present invention have
preferred steric configurations. Accordingly, a preferred steric
configuration is represented by compounds of the formula [I-1]:
6
[0028] wherein n, R.sup.1 through R.sup.6 and X are as defined
above.
[0029] A more preferred steric configuration is represented by
compounds according to the formula [I-2]: 7
[0030] wherein n, R.sup.1 through R.sup.4, R.sup.6 and X are as
defined above and R.sup.5 can be selected from the following: a
--COOH group; a --(C.sub.1-6 alkylene)COOH group, preferably a
--(C.sub.1-3 alkylene)COOH group; a --(C.sub.1-7
alkylene)O(C.sub.1-6 alkyl) group, preferably a --(C.sub.1-4
alkylene)O(C.sub.1-6 alkyl) group or a --(C.sub.1-7
alkylene)O(C.sub.1-3 alkyl) group, more preferably a --(C.sub.1-4
alkylene)O(C.sub.1-3 alkyl) group; a --(C.sub.1-7 alkylene)OH
group, preferably a --(C.sub.1-4 alkylene)OH group; a
--COO(C.sub.1-6 alkyl) group, preferably a --COO(C.sub.1-3 alkyl)
group; a --CONH(C.sub.1-6 alkyl) group, preferably a
--CONH(C.sub.1-3 alkyl) group or a --CONH.sub.2 group.
[0031] In a preferred embodiment of the present invention, R.sup.6
can be selected from the following formula: 8
[0032] In the above, R.sup.7, which occurs one or more times and
which may be the same or different in each occurrence, may be
selected from the following: a --H group; a --OH group; a
--NO.sub.2 group; a --NH.sub.2 group; a --C.sub.1-5 alkyl group,
preferably a --C.sub.1-3 alkyl group; a --F group; a --Cl group; a
--Br group; a --I group; a --COOH group; a --COO(C.sub.1-6 alkyl)
group, preferably a --COO(C.sub.1-3 alkyl) group; a
--O(C.sub.1-C.sub.8 alkyl) group, preferably a --O(C.sub.1-4 alkyl)
group; a CONH(C.sub.1-6 alkylene)COOH group, preferably a
--CONH(C.sub.1-3 alkylene)COOH group; a --OCH.sub.2(C.sub.3-7
cycloalkyl) group, preferably a --OCH.sub.2(C.sub.3-6 cycloalkyl)
group; or a substituent selected from the following formula: 9
[0033] In the above, R.sup.8, which occurs one or more times and
which may be the same or different in each occurrence, may be
selected from the following: a --H group; an --OH group; a
--NH.sub.2 group; a --NO.sub.2 group; a --C.sub.1-7 alkyl group,
preferably a --C.sub.1-4 alkyl group; a --F group; a --Cl group; a
--Br group; a --I group; a --CF.sub.3 group; a phenyl group, or a
--O(C.sub.1-6 alkyl) group, preferably a --O(C.sub.1-3 alkyl)
group.
[0034] In the above, R.sup.9 may be selected from the following: a
--H group; a --Cl alkyl group, preferably a --C.sub.1-3 alkyl
group; a --C.sub.3-7 cycloalkyl group, preferably a --C.sub.3-6
cycloalkyl group; a --(C.sub.1-6alkylene)aryl group, preferably a
--(C.sub.1-3 alkylene)aryl group; an aryl group; or a group
selected from the following formula: 10
[0035] In the above, R.sup.10, which occurs one or more times and
which may be the same or different in each occurrence, may be
selected from the following: a --H group; a --F group; a --Cl
group; a --Br group; an --I group; a --NO.sub.2 group; a
--C.sub.1-6 alkyl group, preferably a --C.sub.1-3 alkyl group; or a
--O(C.sub.1-6 alkyl) group, preferably a --O(C.sub.1-3 alkyl)
group.
[0036] In the above, R.sup.11 may be selected from the following:
11
[0037] In the above, R.sup.12, which occurs one or more times and
which may be the same or different in each occurrence, may be
selected from the following: a --H group; a --CF.sub.3 group; a
--OCH.sub.3 group; a --F group; a --Br group; a --Cl group; or an
--I group;
[0038] The above embodiments carrying the proviso that when R.sup.7
is the formula: 12
[0039] then R.sup.9 is other than hydrogen.
[0040] In a more preferred embodiment of the present invention,
R.sup.6 is selected from the following: 13
[0041] In the above, Y is selected from either a hydrogen atom or a
chlorine atom.
[0042] In a more preferred embodiment of the present invention,
R.sup.2 is selected from the following: a --COOH group or an ester
or an amide thereof; a --CONHCH.sub.2COOH group; a
--CONHOCH.sub.2Ph group; or a --CONHCH.sub.2CONH.sub.2 group.
[0043] In another preferred embodiment of the present invention,
R.sup.1 is a --CH.sub.3 group, and R.sup.2 is a --COOH group; a
--CONHCH.sub.2COOH group; a CONHOCH.sub.2Ph group or a
--CONHCH.sub.2CONH.sub.2 group, and R.sup.3 and R.sup.4 are
hydrogen atoms. Also, X is --CO--, R.sup.5 is --COOH, n is 1,
and
[0044] R.sup.6 is represented by the following formula 14
[0045] wherein R.sup.7 is 15
[0046] and R.sup.8 occurs 2 or 3 times and is a chlorine atom.
[0047] Other compounds within the scope of the present invention
are compounds of the formula [I-3]: 16
[0048] In the above formula [I-3], R.sup.1 may be a hydrogen atom
or a methyl group. Also in the above formula [I-3], R.sup.2 may be
selected from the following: a --CN group; a --COOH group; a
--CONH.sub.2 group; a --CONHOH group; a --CON(CH.sub.3).sub.2
group; a --CH.sub.2OCH.sub.2COOH group; a --CH.dbd.CHCOOH group; a
--CONHCH.sub.2COOH group; a --CONH(CH.sub.2).sub.2COOH group; a
CONHCH.sub.2CONH.sub.2 group; a --CONH(CH.sub.2).sub.2CN group; a
group selected from the following: 17
[0049] In the above formula [I-3], R.sup.3 may be a hydrogen atom
or a methyl group and R.sup.5 may be a --COOH group or a COOMe
group.
[0050] In the above formula [I-3], R.sup.6 may be selected from the
following: 18
[0051] In the above, Z.sup.1 may be selected from the following: a
--O-- group; a --NHCO-- group; a --NHCH.sub.2-- group, a
--OCH.sub.2-- group; a --CONH-group; a --NHSO.sub.2-- group; a
--NHCOCH.sub.2-- group; or a --N(CH.sub.3)CH.sub.2-- group.
[0052] In the above, R.sup.13 may be selected from the following: a
--H group; a -iBuO group; a --CH.sub.3 group; a i-Bu group; or a
group selected from the following: 19
[0053] In the above, R.sup.14, which occurs one or more times and
which may be the same or different in each occurence, may be
selected from the following: a --H group; a --F group; a --Cl
group; a --Br group; an --I group; a --CH.sub.3 group; a
--OCH.sub.3 group; a --CF.sub.3 group; a --NO, group; a --NH.sub.2
group; or a -n-C.sub.7H.sub.15 group.
[0054] In the above, R.sup.15 may be selected from the following: a
--H group; a --OH group; a --NO.sub.2 group; or a group selected
from the following: 20
[0055] In the above, R.sup.16 may be selected from the following
21
[0056] In the above, R.sup.17, which occurs one or more times and
which may be the same of different in each occurrence, may be
selected from the following: a --H group; a --Cl group; a
--OCH.sub.3 group; or a --CF.sub.3 group, provided that R.sup.1 and
R.sup.3 must be different.
[0057] In a another embodiment of the compounds according to
formula [I-3], R.sup.1 is a hydrogen atom or a methyl group, and
R.sup.2 is selected from the following: a --CN group; a --COOH
group; a --COOMe group; a --CONH.sub.2 group; a --CONHOH group; a
--CON(CH.sub.3).sub.2 group; a --CH.sub.2OCH.sub.2COOH group; a
--CH.dbd.CHCOOH group; a --CONHCH.sub.2COOH group; a
--CONH(CH.sub.2).sub.2COOH group; a --CONHCH.sub.2CONH.sub.2 group;
a --CONH(CH.sub.2).sub.2CN group; or a group selected from the
following: 22
[0058] and R.sup.3 is a hydrogen atom or a methyl group.
[0059] In the another embodiment of formula [I-3], R.sup.5 is a
--COOH group or a --COOMe group and R.sup.6 is selected from the
following: 23
[0060] In the above embodiment of formula [I-3], Z.sup.1 may be
selected from the following: a --O-- group; a --NHCO-- group; a
--NHCH.sub.2-- group; a --OCH.sub.2-- group; a --CONH-- group; a
--NHSO.sub.2-- group; or a --NHCOCH.sub.2-- group.
[0061] In the above embodiment of formula [I-3], R.sup.13 may be
selected from the following: a --H group; a -iBuO group; a -i-Bu
group; or a group selected from the following: 24
[0062] In the above embodiment of formula [I-3], R.sup.14, which
occurs one or more times and which may be the same or different in
each occurence, may be selected from the following: a --H group; a
--F group; a --Cl group; a --Br group; an --I group; a --CH.sub.3
group; a --OCH.sub.3 group; a --CF.sub.3 group; a --NO.sub.2 group;
a --NH, group; or a -n-C.sub.7H.sub.15 group.
[0063] In the above embodiment of formula [I-3], R.sup.15 may be
selected from the following: a --H group; a --OH group; a
--NO.sub.2 group; or a group selected from the following: 25
[0064] In the above embodiment of formula [I-3], R.sup.16 may be
selected from the following: 26
[0065] In the above embodiment of formula [I-3] R.sup.17, which
occurs one or more times and which may be the same of different in
each occurrence, is a chlorine atom, provided that R.sup.1 and
R.sup.3 must be different.
[0066] In another embodiment of formula [I-3], R.sup.1 is methyl
group and R.sup.2 may be selected from the following: a --CN group;
a --COOH group; a --CONH.sub.2 group; a --CONHOH group; a
--CH.sub.2OCH.sub.2COOH group; a --CH.dbd.CHCOOH group; a
--CONHCH.sub.2COOH group; a --CONH(CH.sub.2).sub.2COOH group; a
--CONHCH.sub.2CONH.sub.2 group; a --CONH(CH.sub.2).sub.2CN group;
or a group selected from the following: 27
[0067] In the above, R.sub.3 is a hydrogen atom, R.sup.5 is a
--COOH group or a --COOMe group and R.sup.6 may be selected from
the following: 28
[0068] In the above embodiment of formula [I-3], Z.sup.1 may be
selected from the following: a --NHCO-- group; a --OCH.sub.2-group;
a --NHCH.sub.2-- group; a --CONH-- group; or a
--NHSO.sub.2-group.
[0069] In the above embodiment of formula [I-3], R.sup.13 may be
selected from the following: 29
[0070] In another embodiment of formula [I-3], R.sup.14, which
occurs one or more times and which may be the same or different in
each occurence, may be selected from the following: a --F group; a
--Cl group; a --Br group; an --I group; a --CH.sub.3 group; a
--OCH.sub.3 group; a --CF.sub.3 group; or a --NO.sub.2 group.
[0071] In another embodiment of formula [I-3], R.sup.15 may be a
--H group or a --OH group.
[0072] In a another embodiment of formula [I-3], R.sup.1 is a
hydrogen atom or a methyl group and R.sup.2 may be selected from
the following: a --CN group; a --COOH group; a --CONH.sub.2 group;
a --CONHOH group; a --CONHOCH.sub.3 group; a
--CH.sub.2OCH.sub.2COOH group; a --CH.dbd.CHCOOH group; a
--CONHCH.sub.2COOH group; a --CONH(CH.sub.2).sub.2COOH group; a
--CONHCH.sub.2CONH.sub.2 group; a --CONH(CH.sub.2).sub.2CN group;
or a group selected from the following: 30
[0073] In another embodiment of formula [I-3], R.sup.3 is a
hydrogen atom or a methyl group, R.sup.5 is a --COOH group or a
--COOMe group, and R.sup.6 may be selected from the following:
31
[0074] In another embodiment of formula [I-3], Z.sup.1 may be
selected from the following: a --O-- group; a --NHCO-- group; a
--NHCH.sub.2-- group; a --OCH.sub.2-- group; a --CONH-- group; or a
--NHSO.sub.2-- group.
[0075] In another embodiment of formula [I-3], R.sup.13, may be
selected from the following: a --H group; a -iBuO group; or a group
selected from the following: 32
[0076] In another embodiment of formula [I-3], R.sup.14, which
occurs one or more times and which may be the same or different in
each occurence, may be selected from the following: a --H group; a
--OH; a --F group; a --Cl group; a --Br group; an --I group; a
--CH.sub.3 group; a --OCH.sub.3 group; a --CF.sub.3 group; a
--NO.sub.2 group; or a --NH.sub.2 group.
[0077] In another embodiment of formula [I-3], R.sup.15 may be
selected from the following: a --H group; a --OH group; a
--NO.sub.2 group; or a group selected from the following: 33
[0078] In another embodiment of formula [I-3], R.sup.16 may be
selected from the following: 34
[0079] In another embodiment of formula [I-3], R.sup.17, which
occurs one or more times and which may be the same or different in
each occurrence, may be a --Cl group or a --CF.sub.3 group,
provided that R.sup.1 and R.sup.3 must be different.
[0080] In another embodiment of formula [I-3], R.sup.1 may be a
hydrogen atom or methyl group, R.sup.2 may be selected from the
following: a --CN group; a --COOH group; a --CONH.sub.2 group; a
--CONHOH group; a --CH.sub.2OCH.sub.2COOH group; a --CH.dbd.CHCOOH
group; a --CONHCH.sub.2COOH group; a --CONH(CH.sub.21).sub.2COOH
group; a --CONHCH.sub.2CONH.sub.2 group; a --CONH(CH.sub.2).sub.2CN
group; or a group selected from the following: 35
[0081] and R.sup.3 may be a hydrogen atom or a methyl group.
[0082] In another embodiment of formula [I-3], R.sup.1 is --COOH
group or a --COOMe group and R.sup.6 may be selected from the
following: 36
[0083] In another embodiment of formula [I-3], Z.sup.1 may be
selected from the following: a --O-- group; a --NHCO-- group; a
--NHCH.sub.2-- group; a --OCH.sub.2-- group; a --CONH-- group; or a
--NHSO.sub.2-- group.
[0084] In another embodiment of formula [I-3], R.sup.13 may be a
--H group or a group selected from the following: 37
[0085] In another embodiment of formula [I-3], R.sup.14, which
occurs one or more times and which may be the same or different in
each occurence, may be selected from the following: a --H group; a
--F group; a --Cl group; a --Br group; an --I group; a --CH.sub.3
group; a --OCH.sub.3 group; a --CF.sub.3 group; a --NO.sub.2 group;
or a --NH.sub.2 group.
[0086] In another embodiment of formula [I-3], R.sup.15 may be
selected from the following: a --H group; a --OH group, a
--NO.sub.2 group; or a group selected from the following: 38
[0087] In another embodiment of formula [I-3], R.sup.16 may be
selected from the following: 39
[0088] In another embodiment of formula [I-3], R.sup.17, which
occurs one or more times and which may be the same or different in
each occurrence, may be a --H group or a --Cl group, provided that
R.sup.1 and R.sup.3 must be different.
[0089] In another embodiment of formula [I-3], R.sup.1 is a methyl
group, and R.sup.2 may be selected from the following: a --CN
group; a --COOH group; a --CONH.sub.2 group; a --CONHOH group; a
--CH.sub.2OCH.sub.2COOH group; a --CH.dbd.CHCOOH group; a
--CONHCH.sub.2COOH group; a --CONH(CH.sub.2).sub.2COOH group; a
--CONH(CH.sub.2).sub.2CN group; a --CONHCH.sub.2CONH.sub.2 group;
or a group selected from the following: 40
[0090] In another embodiment of formula [I-3], R.sup.3 is a
hydrogen atom, R.sup.5 is a --COOH group or a --COOMe group, and
R.sup.6 may be selected from the following: 41
[0091] In another embodiment of formula [I-3], Z.sup.1 may be
selected from the following: a --NHCO-- group; a --NHCH.sub.2--
group; a --NAcCH.sub.2-- group; a --OCH.sub.2-- group; or a
--CONH-group.
[0092] In another embodiment of formula [I-3], R.sup.13 may be
selected from the following: 42
[0093] In another embodiment of formula [I-3], R.sup.14, which
occurs one or more times and which may be the same or different in
each occurence, may be selected from the following: a --F group; a
--Cl group; a --Br group; an --I group; a --OCH.sub.3 group; a
--CF.sub.3 group; or a --NO.sub.2 group.
[0094] In another embodiment of formula [I-3], R.sup.1 is a --H
group or a --NO.sub.2 group.
[0095] Preferred compounds according to formula [I] may be selected
from the group consisting of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl-
)-carbonyl]-1-[(3,4-dichlorophenyl)methyl]-L-histidine,
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichl-
orophenyl)methyl]-L-tyrosine,
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclop-
entyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine,
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichl-
orophenyl)methyl]-3-nitro-L-tyrosine,
(1S-cis)-N-[(3-Carboxy-2,2,3-trimeth-
ylcyclopentyl)carbonyl]-4-[[(2,4,6-trichlorophenyl)carbonyl]-amino]-L-phen-
ylalanine,
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trim-
ethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine,
[0096]
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcycl-
opentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine,
and
(1S-cis)-N-[(3-Cyano-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlor-
obenzoyl)amino]-L-phenylalanine.
[0097] The desired compounds of the present invention may exist in
the form of optical isomers based on asymmetric carbon atoms
thereof, and the present invention also includes these optical
isomers and mixtures thereof.
[0098] In an embodiment of the present invention, the steric
configuration of a bond need not be fixed. A bond may be of any
acceptable configuration. Further, a compound may be a mixture with
several different configurations of the same bond.
[0099] The desired compounds of the present invention may be used
in the form of an ester or amide thereof. As the ester thereof,
there may be mentioned a C.sub.1-6 alkyl ester, a C.sub.2-7 alkenyl
ester, a C.sub.2-7 alkynyl ester, a C.sub.2-7 alkanoyloxy-C.sub.1-6
alkyl ester, an aryl-C.sub.1-6 alkyl ester or an aryl ester. As the
amide thereof, there may be mentioned an, amide (--CONH.sub.2), a
mono or di N--C.sub.1-6 alkyl amide, an N--C.sub.3-8 cycloalkyl
amide, an N-aryl amide or an N-aryl-C.sub.1-6 alkyl amide.
[0100] The desired compound of the present invention may be
clinically used either in a free form or in the form of
pharmaceutically acceptable salts thereof. Pharmaceutically
acceptable salts include acid-addition salts with inorganic acid or
organic acid (e.g., hydrochloride, sulfate, nitrate, hydrobromide,
methanesulfonate, p-toluenesulfonate, acetate), salt with inorganic
base, organic base or amino acid (e.g., triethylamine salt, a salt
with lysine, an alkali metal salt, an alkali earth metal salt and
the like).
[0101] The compound may also be formulated into a pharmaceutical
composition comprising a therapeutically effective amount of the
compound as defined above and a pharmaceutically acceptable carrier
or diluent.
[0102] The compound can also be used for treating or preventing
.alpha..sub.4.beta..sub.1 adhesion mediated conditions in a mammal
such as a human. This method may comprise administering to a mammal
or a human patient an effective amount of the compound or
composition as explained above.
[0103] This method can be used to treat such inflammatory
conditions as rheumatoid arthritis, asthma, allergy conditions,
adult respiratory distress syndrome, AIDS, cardiovascular diseases,
thrombosis or harmful platelet aggregation, reocclusion following
thrombolysis, allograft rejection, reperfusion injury, psoriasis,
eczema, contact dermatitis and other skin inflammatory diseases,
osteoporosis, osteoarthritis, atherosclerosis, neoplastic diseases
including metastasis of neoplastic or cancerous growth, wound
healing enhancement, treatment of certain eye diseases such as
detaching retina, Type I diabetes, multiple sclerosis, systemic
lupus erythematosus (SLE), inflammatory and immunoinflammatory
conditions including ophthalmic inflammatory conditions and
inflammatory bowel diseases, ulcerative colitis, atherosclerosis,
regional enteritis and other autoimmune diseases.
[0104] The desired compound of the present invention or
pharmaceutically acceptable salts thereof may be administered
either orally or parenterally, and it may be used as a suitable
pharmaceutical preparation, for example, a tablet, a granule, a
capsule, a powder, an injection, and an inhalation by a
conventional process.
[0105] The dose of the desired compound of the present invention or
a pharmaceutically acceptable salt thereof varies depending on an
administration method, age, body weight, and state of a patient,
but, in general, the daily dose is preferably about 0.1 to 100
mg/kg/day, particularly preferably 1 to 100 mg/kg/day.
[0106] Preferred Routes of Administration for Asthma:
[0107] It is preferred that the compound of the present invention
be administered in the form of an Aerosol. However, other routes of
administration include intravenous, oral, intramuscular, and
subcutaneous.
[0108] In the case of aerosol administration, compositions
containing the compounds of the present invention can be prepared
to provide for an excellent means for administering in aerosol form
for inhalation therapy. Accordingly, the present invention will
provide for self-propelling compositions containing the compounds
of the present invention.
[0109] Propellants employed should be non-toxic and have a vapor
pressure suitable for the conditions under which administration
occurs. These propellants can be fluorinated or fluorochlorinated
lower saturated aliphatic hydrocarbons. The preferred propellants
of this type are the halogenated alkanes containing not more than
two carbon atoms and at least one fluorine atom. Illustrative of
these are trichloromonofluoromethane, dichlorodifluoromethane,
monochlorotrifluoromethane, dichloromonofluoromethane and
1,2-dichloro-1,1,2,2-tetrafluoroethane. These compounds are
available from E. I. duPont de Nemours and Company under the trade
name "Freon". These propellants may be employed singularly or in
admixture.
[0110] In addition to the propellant, an organic solvent may also
be employed. The organic solvent must be non-toxic and without
undesirable effects on inhalation in the amount present in the
aerosol produced. In addition, the solvent should be substantially
anhydrous, completely miscible with the propellant or mixture of
propellants employed and have a suitable boiling point. Examples of
such solvents included non-toxic aliphatic alcohols such as
ethanol; ethers such as ethyl ether and vinyl ether; ketones such
as acetone; and suitable halogenated lower alkanes.
[0111] In addition to the organic solvent, the composition may also
optionally contain a non-toxic hygroscopic glycol. The glycol must
be substantially miscible with the organic solvent and the
propellant employed. Satisfactory glycols include propylene glycol,
triethylene glycol, glycerol, butylene glycol and hexylene
glycol.
[0112] The above indicated methods of admistration and formulation
of aerosol compositions should not be viewed as limiting. The
compounds of the present invention can be formulated in anyway
deemed suitable to one of ordinary skill in the art so as to obtain
the desired effects.
[0113] Pharmaceutical Compositions
[0114] As indicated previously, the compounds of formula (I) can be
formulated into pharmaceutical compositions. In determining when a
compound of formula (I) is indicated for the treatment of a given
disease, the particular disease in question, its severity, as well
as the age, sex, weight, and condition of the subject to be
treated, must be taken into consideration and this perusal is to be
determined by the skill of the attendant physician.
[0115] For medical use, the amount of a compound of Formula (I)
required to achieve a therapeutic effect will, of course, vary both
with the particular compound, the route of administration, the
patient under treatment, and the particular disorder or disease
being treated. A suitable daily dose of a compound of Formula (I),
or a pharmaceutically acceptable salt thereof, for a mammalian
subject suffering from, or likely to suffer from, any condition as
described hereinbefore is 0.1 mg to 100 mg of the compound of
formula I, per kilogram body weight of the mammalian subject. In
the case of systematic administration, the dose may be in the range
of 0.5 to 500 mg of the compound per kilogram body weight, the most
preferred dosage being 0.5 to 50 mg/kg of mammal body weight
administered two to three times daily. In the case of topical
administration, e.g., to the skin or eye, a suitable dose may be in
the range of 0.1 .mu.g to 100 .mu.g of the compound per kilogram,
typically about 0.1 .mu.g/kg.
[0116] In the case of oral dosing, a suitable dose of a compound of
Formula (I), or a physiologically acceptable salt thereof, may be
as specified in the preceding paragraph, but most preferably is
from 1 mg to 10 mg of the compound per kilogram, the most preferred
dosage being from 1 mg to 5 mg/kg of mammal body weight, for
example, from 1 to 2 mg/kg. Most preferably, a unit dosage of an
orally administrable composition encompassed by the present
invention contains less than about 1.0 g of a formula (I)
compound.
[0117] It is understood that formulation, both for human and
veterinary use, of the present invention may be presented to the
mammal by inhalation. To achieve therapeutic effect, the dose may
be in the range of 0.5 to 500 mg of the compound, per kg body
weight. The most preferred dosage being 0.5 to 50 mg/kg of mammal
body weight administered two to three times daily.
[0118] It is understood that the ordinarily skilled physician or
veterinarian will readily determine and prescribe the effective
amount of a compound of Formula I to prevent or arrest the progress
of the condition for which treatment is administered. In so
proceeding, the physician or veterinarian could employ relatively
low doses at first, subsequently increasing the dose until a
maximum response is obtained.
[0119] The compounds and compositions of the present invention can
be administered to patients suffering from a condition listed
herein in an amount which is effective to fully or partially
alleviate undesired symptoms of the condition. The symptoms may be
caused by inappropriate cell adhesion mediated by 0401 integrins.
Such inappropriate cell adhesion would typically be expected to
occur as a result of increased VCAM-1 and/or CS-1 expression on the
surface of endothelial cells. Increased VCAM-1 and/or CS-1
expression can be due to a normal inflammation response or due to
abnormal inflammatory states. In either case, an effective dose of
a compound of the invention may reduce the increased cell adhesion
due to increased VCAM-1 expression by endothelial cells. Reducing
the adhesion observed in the disease state by 50% can be considered
an effective reduction in adhesion. More preferably, a reduction in
adhesion by 90%, is achieved. Most preferably adhesion mediated by
VCAM-1/U40, and/or CS-1 interaction is abolished by an effective
dose. Clinically, in some instances, effect of the compound can be
observed or a decrease in white cell infiltration into tissues or a
site of injury. To achieve a therapeutic effect, then, the
compounds or compositions of the present invention are administered
to provide a dose effective to reduce or eliminate inappropriate
cell adhesion or to alleviate undesired symptoms.
[0120] While it is possible for an active ingredient to be
administered alone, it is preferable to present it as a
pharmaceutical formulation comprising a compound of Formula (I) and
a pharmaceutically acceptable carrier thereof. Such formulations
constitute a further feature of the present invention.
[0121] The formulations, both for human and veterinary medical use,
of the present invention comprise an active ingredient of Formula
(I), in association with a pharmaceutically acceptable carrier
thereof and optionally other therapeutic ingredient (s), which are
generally known to be effective in treating the disease or
condition encountered. The carrier(s) must be "acceptable" in the
sense of being compatible with the other ingredients of the
formulations and not deleterious to the recipient thereof.
[0122] The formulations include those in a form suitable for oral,
pulmonary, ophthalmic, rectal, parenteral (including subcutaneous,
intramuscular, and intravenous), intra-articular, topical, nasal
inhalation (e.g., with an aerosol) or buccal administration. Such
formulation are understood to include long-acting formulations
known in the art.
[0123] The formulations may conveniently be presented in unit
dosage form and may be prepared by any of the methods well known in
the art of pharmacy. All methods may include the step of bringing
the active ingredient into association with the carrier which
constitutes one or more accessory ingredients. In general, the
formulations are prepared by uniformly and intimately bringing the
active ingredient into association with a liquid carrier or a
finely divided solid carrier or both, and then, if necessary,
shaping the product into the desired form.
[0124] Formulations of the present invention suitable for oral
administration may be in the form of discrete units such as
capsules, cachets, tablets, or lozenges, each containing a
predetermined amount of the active ingredient in the form of a
powder or granules; in the form of a solution or suspension in an
aqueous liquid. Formulations for other uses could involve a
nonaqueous liquid; in the form of an oil-in-water emulsion or a
water-in-oil emulsion; in the form of an aerosol; or in the form of
a cream or ointment or impregnated into a transdermal patch for use
in administering the active ingredient transdermally, to a patient
in need thereof. The active ingredient of the present inventive
compositions may also be administered to a patient in need thereof
in the form of a bolus, electuary, or paste.
[0125] The practitioner is referred to "Remington: The Science and
Practice of Pharmacy," 19th Edition, c. 1995 by the Philadelphia
College of Pharmacy and Science, as a comprehensive tome on
pharmaceutical preparations.
1 Abbreviations Ac.sub.2O: Acetic anhydride AcOEt: Ethyl acetate
BCECF-AM: 2',7'-bis-(2-carboxyethyl)-5-(and 6-) carboxyfluorescein
acetoxymethyl ester BOP-Cl: Bis (2-oxo-3-oxazolidinyl) phosphinic
chloride BOP Reagent: Benzotriazol-1-yloxy-tris
(dimethylamino)-phosphonium hexafluorophosphate DMEM: Dulbecco's
Minimal Eagle's Media DMF: Dimethyl formamide DIEA:
Diisopropylethylamine EDC: 1-(3-Dimethylaminopropyl)-3-
ethylcarbodiimide hydrochloride Et: Ethyl EtOH: Ethanol HATU:
N-[(Dimethylamino)-1H-1,2,3-triazolo[4,5-
b]-pyridin-1-ylmethylene]-N- methylmethanaminium
hexafluorophosphate N-oxide HBSS: Hank's Balanced Salt Solution
HBTU: O-Benzotriazol-1-yl-N,N,N',N'- tetramethyluronium
hexafluorophosphate HOBT: 1-Hydroxybenzotriazole HSA: Human serum
albumin LDA: Lithium diisopropylamide Me: Methyl meq:
milliequivalent MeOH: Methanol n-Bu: n-Butyl NMP:
1-Methyl-2-pyrrolidinone PBS: Phosphate buffered saline Pd-C:
Palladium on charcoal Ph: Phenyl SPDP: 3-(2-pyridyldithio)
propionic acid N- hydroxysuccinimide ester t-Bu: t-butyl THF:
Tetrahydrofuran TFA: Trifluoroacetic acid
[0126] According to the present invention, the desired compound [I]
can be prepared by the following methods: 43
[0127] wherein R.sup.5a is a group of the formula: --COOR.sup.22,
--(C.sub.1-6 alkylene)COOR.sup.22, C.sub.1-7 alkylene)O(C.sub.1-6
alkyl), --(C.sub.1-7alkylene) OH, --COO(C.sub.1-6
alkyl)-CONH(C.sub.1-6 alkyl) or --CONH.sub.2,
[0128] R.sup.22 is a protecting group for the carboxyl group, and
the other symbols are the same as defined above.
[0129] Method A:
[0130] The compound of the formula [I] wherein R.sup.2 is a group
of the formula: --COOH and X is a group of the formula: --CO--, an
ester thereof, an amide thereof or a pharmaceutically acceptable
salt thereof, i.e., the compound of the formula [I-a]: 44
[0131] wherein the symbols are the same as defined above, an ester
thereof, an amide thereof or a pharmaceutically acceptable salt
thereof may be prepared by
[0132] (1) reacting a compound of the formula [II]: 45
[0133] wherein the symbols are the same as defined above, with a
compound of the formula [III-a]: 46
[0134] wherein the symbols are the same as defined above, or a salt
thereof,
[0135] (2) removing the protecting group for the carboxyl group, if
desired, and
[0136] (3) converting the resulting compound into an ester thereof,
an amide thereof or a pharmaceutically acceptable salt thereof by a
conventional method, if further desired.
[0137] R.sup.22 can be selected from a conventional protecting
group for a carboxyl group, for example, a C.sub.1-6 alkyl group, a
C.sub.2-7 alkenyl group, a C.sub.2-7 alkynyl group, a C.sub.2-7
alkanoyloxy-C.sub.1-6 alkyl group, an aryl-C.sub.1-6 alkyl group
(e.g., benzyl group) or an aryl group (e.g., phenyl group) and the
like.
[0138] A salt of the compound [III-a] includes, for example, salt
with an inorganic acid (e.g., hydrochloride, sulfate) and salt with
an inorganic base (e.g., an alkali metal salt such as sodium salt
or potassium salt, an alkali earth metal salt such as magnesium
salt or calcium salt).
[0139] The reaction of the compound [II] and the compound [III-a]
or a salt thereof is carried out in the presence of a base in a
suitable solvent or without a solvent. The base can be selected
from an organic base (e.g., DIEA, DMAP, Et.sub.3N, DBU), an alkali
metal hydride (e.g., NaH, LiH), an alkali metal carbonate (e.g.,
Na.sub.2CO.sub.3, Na.sub.2KO.sub.3) an alkali metal hydrogen
carbonate (e.g., NaHCO.sub.3, KHCO.sub.3), an alkali metal amide
(e.g., NaNH.sub.2), an alkali metal alkoxide (e.g., NaOMe, KOMe),
an alkyl-alkali metal (n-BuLi, t-BuLi), an alkali metal hydroxide
(e.g., NaOH, KOH), an alkali earth metal hydroxide (e.g.,
Ba(OH).sub.2), and the like. The solvent can be selected from any
one which does not disturb the reaction, for example, DMF, THF,
benzene, toluene, DMSO, CH.sub.3CN or a mixture thereof. The
reaction is preferably carried out at a temperature from 0.degree.
C. to 100.degree. C., more preferably at a temperature from 400% to
80.degree. C.
[0140] The removal of said protecting group from the products can
be carried out by a conventional method, which is selected
according to the types of the protecting groups to be removed, for
example, hydrolysis, acid treatment, catalytic reduction, and the
like.
[0141] A more preferred method than method A is: 47
[0142] R.sup.2a is a group of the formula: --CN, --COOR.sup.23,
--COOH, --(C.sub.1-6 alkylene)OH, --CH.sub.2O(C.sub.1-6 alkyl),
--(C.sub.1-6 alkylene)COOH, --(C.sub.1-6 alkylene)COOR.sup.23,
--CH.sub.2C(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl),
--CH.sub.2O(C.sub.1-6 alkylene) COOH, --CH.sub.2O(C.sub.1-6
alkylene) COOR.sup.23, --(C.sub.2-7 alkenylene) COOH, --(C.sub.2-7
alkenylene) COOR.sup.23, --CO(C.sub.1-6 alkylene)COOH,
--CO(C.sub.1-6 alkylene)COOR.sup.23, --CO(C.sub.2-7
alkenylene)COOH, --CO(C.sub.2-7 alkenylene)COOR.sup.23,
--CO(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl), --CO(C.sub.1-6
alkylene)CO(C.sub.1-6 alkyl), --CONH(C.sub.1-6 alkyl),
--CONHO(C.sub.1-6 alkyl), --CONH(C.sub.1-6 alkylene) COOH,
--CONH(C.sub.1-6 alkylene)COOR.sup.23, --CONH(C.sub.3-7
cycloalkyl), --CONH.sub.2, --CONH(C.sub.1-6 alkylene)CONH.sub.2,
--CONHOH, --NHCO.sub.2CH.sub.2Ph, --CONHOCH.sub.2Ph,
--CONH(C.sub.1-6alkylene) CN, --COO(C.sub.1-6alkyl),
--CH.sub.2O(C.sub.1-6alkylene) CONH.sub.2, 48
[0143] R is a group of the formula: --CN, --COOR.sup.23,
--(C.sub.1-6 alkylene) OH, --CH.sub.2O(C.sub.1-6 alkyl),
--(C.sub.1-6 alkylene) COOR.sup.23, --CH.sub.2O(C.sub.1-6
alkylene)O(C.sub.1-6 alkyl), --CH.sub.2O(C.sub.1-6
alkylene)COOR.sup.23, --(C.sub.2-7 alkenylene) COOR.sup.23,
--CO(C.sub.1-6 alkylene)COOR.sup.23, --CO(C.sub.2-7 alkenylene)
COOR.sup.23, --CO(C.sub.1-6 alkylene)O(C.sub.1-6 alkyl),
--CO(C.sub.1-6 alkylene)CO(C.sub.1-6 alkyl), --CONH(C.sub.1-6
alkyl), --CONHO(C.sub.1-6 alkyl), --CONH(C.sub.1-6
alkylene)COOR.sup.23, --CONH(C.sub.3-7 cycloalkyl), --CONH.sub.2,
--CONH(C.sub.1-6 alkylene) CONH.sub.2, --CONHOH,
--NHCO.sub.2CH.sub.2Ph, --CONHOCH.sub.2Ph,
--CONH(C.sub.1-6alkylene) CN, --COO(C.sub.1-6alkyl)
--CH.sub.2O(C.sub.1-6alkylene) CONH.sub.2, 49
[0144] R.sup.23 is a protecting group for the carboxyl group, and
the other symbols are the same as defined above.
[0145] Method B:
[0146] The compound of the formula [II, an ester thereof, an amide
thereof or a pharmaceutically acceptable salt thereof,
[0147] wherein R.sup.2 is a group of the formula: --CN,
--COOR.sup.23, --COOH, --(C.sub.1-6 alkylene) OH,
--CH.sub.2O(C.sub.1-6 alkyl), --(C.sub.1-6 alkylene)COOH,
--(C.sub.1-6 alkylene)COOR.sup.23, --CH.sub.2O(16
alkylene)O(C.sub.1-16 alkyl), --CH.sub.2O(C.sub.1-6 alkylene)COOH,
--CH.sub.2O(C.sub.1-16 alkylene)COOR.sup.23, --(C.sub.2-7
alkenylene)COOH, --(C.sub.2-7 alkenylene)COOR.sup.23,
--CO(C.sub.1-16 alkylene)COOH, --CO(C.sub.1-16
alkylene)COOR.sup.23, --CO(C.sub.2-7 alkenylene)COOH,
--CO(C.sub.1-27 alkenylene)COOR.sup.23, --CO(C.sub.1-6
alkylene)O(C.sub.1-6 alkyl), --CO(C.sub.1-6 alkylene)CO(C.sub.1-6
alkyl), --CONH(C.sub.1-6 alkyl), --CONHO(C.sub.1-6 alkyl),
--CONH(C.sub.1-16 alkylene) COOH, --CONH(C.sub.1-16
alkylene)COOR.sup.23, --CONH(C.sub.3-7 cycloalkyl), --CONH.sub.2,
--CONH(C.sub.1-6 alkylene)CONH.sub.2, --CONHOH,
--NHCO.sub.2CH.sub.2Ph, CONHOCH.sub.2Ph, --CONH(C.sub.1-6alkylene)
CN, --COO(C.sub.1-6alkyl), --CH.sub.2O(C.sub.1-6alkylene)
CONH.sub.2 or 50
[0148] X is a group of the formula: --CO--, i.e., the compound of
the formula-[I-c]: 51
[0149] wherein
[0150] the symbols are the same as defined above, may be prepared
by
[0151] (1) condensing a compound of the formula [IV]: 52
[0152] wherein the symbols are the same as defined above, or a salt
thereof, with a compound of the formula [III-a]: 53
[0153] wherein the symbols are the same as defined above, or a salt
thereof,
[0154] (2) removing the protecting group for the carboxyl group and
hydroxyl group, if desired, and
[0155] (3) converting the resulting compound into an ester thereof,
an amide thereof or a pharmaceutically acceptable salt thereof by a
conventional method, if further desired.
[0156] R.sup.22 and R.sup.23 are the same or different conventional
protecting group for a carboxyl group, for example, a C.sub.1-6
alkyl group, a C.sub.2-7 alkenyl group, a C.sub.2-7 alkynyl group,
a C.sub.2-7 alkanoyloxy-C.sub.1-6 alkyl group, an aryl-C.sub.1-6
alkyl group (e.g., benzyl group) or an aryl group (e.g., phenyl
group) and the like.
[0157] A salt of the compound [III-a] and/or [IV] includes, for
example, salt with an inorganic acid (e.g., hydrochloride, sulfate)
and salt with an inorganic base (e.g., an alkali metal salt such as
sodium, potassium and calcium, an alkali earth metal salt such as
barium).
[0158] The condensation reaction of the compound [IV] or a salt
thereof with the compound [III-a] or a salt thereof is carried out
in the presence of a condensing reagent in a suitable solvent or
without a solvent. The condensing reagent can be selected from any
one which can be used for a conventional peptide synthesis, for
example, BOP-Cl, BOP reagent, DCC and WSCI.
[0159] The solvent can be selected from any one which does not
disturb the condensation reaction, for example, CH.sub.2Cl.sub.2,
DMF or a mixture thereof. The reaction is preferably carried out at
a room temperature.
[0160] The removal of said protecting group from the products can
be carried out by a conventional method, which is selected
according to the types of the protecting groups to be removed, for
example, hydrolysis, acid treatment, catalytic reduction, and the
like. 54
[0161] wherein the symbols are the same as defined above.
[0162] Method C:
[0163] The compound of the formula [I] wherein X is a methylene
group, an ester thereof, an amide thereof or a pharmaceutically
acceptable salt thereof, i.e., the compound of the formula [I-e]:
55
[0164] wherein
[0165] the symbols are the same as defined above, an ester thereof,
an amide thereof or a pharmaceutically acceptable salt thereof, may
be prepared by
[0166] (1) reacting a compound of the formula [V]: 56
[0167] wherein the symbols are the same as defined above, or a salt
thereof in the presence of a reducing agent with a compound of the
formula [III-b]: 57
[0168] wherein the symbols are the same as defined above,
[0169] (2) removing the protecting group for the carboxyl group, if
desired, and
[0170] (3) converting the resulting compound into an ester thereof,
an amide thereof or a pharmaceutically acceptable salt thereof by a
conventional method, if further desired.
[0171] A salt of the compound [V] and/or [III-b] includes, for
example, salt with an inorganic acid (e.g., hydrochloride, sulfate)
and salt with an inorganic base (e.g., an alkali metal salt, an
alkali earth metal salt).
[0172] The reductive alkylation of the compound [V] or a salt
thereof with the compound [III-b] or a salt thereof is carried out
by a conventional method in the presence of a reducing agent in a
suitable solvent or without a solvent. The reducing agent is
preferably sodium borohydride, sodium cyanoborohydride, and the
like. The solvent can be selected from any one which does not
disturb the reaction, for example, alkanol such as methanol,
alkanoic acid such as AcOH, THF or a mixture thereof. The reaction
is preferably carried out at a temperature from 0.degree. C. to a
room temperature.
[0173] The reaction of the compound [I-f] or a salt thereof and the
compound [VII] is carried out in the presence of an acid acceptor
in a suitable solvent or without a solvent. The acid acceptor and
the solvent can be selected from the base or the solvent used in
Method A. The reaction is preferably carried out at room
temperature.
[0174] The removal of said protecting group from the products can
be carried out by a conventional method, which is selected
according to the types of the protecting groups to be removed, for
example, hydrolysis, acid treatment, catalytic reduction, and the
like.
[0175] The desired compound [I] of the present invention can be
converted to each other. Such conversion of the present compound
[I] into the other compound [I] may be carried out by selecting one
of the following procedures from (a) to (e) according to the type
of substituent thereof, and if desired, followed by removing the
protecting group for the carboxyl group by a conventional
method.
[0176] Procedure (a):
[0177] The compound [I] wherein R.sup.6 is an amino-substituted
aryl group can be prepared by the reduction of the compound [I]
wherein the corresponding R.sup.6 is an aralklyoxycarbonyl amino
group- or nitro-substituted aryl group. The reduction can be, for
example, a, catalytic reduction using a palladium catalyst such as
palladium on an activated carbon, a platinum catalyst such as
platinum oxide, and the like. The catalytic reduction is preferably
carried out at a room temperature.
[0178] Procedure (b):
[0179] The compound [I] wherein R.sup.2 is a group of the formula:
--CONH.sub.2, --CONH(C.sub.1-6 alkyl), --CONHO(C.sub.1-6 alkyl),
--CONH(C.sub.1-6 alkylene)COOR.sup.23, --CONH(C.sub.3-7
cycloalkyl), --CONH(C.sub.1-6 alkylene) CONH.sub.2,
--CONHOCH.sub.2Ph, --CONH(C.sub.1-6 alkylene)CN, CONHOH, 58
[0180] can be prepared by reacting the compound [I] wherein the
corresponding R.sup.2 is a group of the formula: --COOH with a
substituted or unsubstituted amine selected from a group of the
formula: NH.sub.3, NH.sub.2(C.sub.1-6 alkyl), NH.sub.2O(C.sub.1-6
alkyl), NH.sub.2(C.sub.1-6 alkylene)COOR.sup.23, NH.sub.2(C.sub.3-7
cycloalkyl), NH.sub.2(C.sub.1-6 alkylene)CONH.sub.2, NH.sub.2OH,
NH.sub.2OCH.sub.2Ph, NH.sub.2 (C.sub.1-6 alkylene)CN, 59
[0181] wherein R.sup.23 is defined as above, in the presence of a
condensing reagent (e.g., BOP reagent) which can be used for a
conventional peptide synthesis, and removing the protecting group
for the carboxyl group, if desired. The reaction is preferably
carried out at a temperature from 0.degree. C. to a room
temperature.
[0182] Procedure (c):
[0183] The compound [I] wherein R.sup.4 is a C.sub.1-6 alkyl group
can be prepared by reacting the compound [I] wherein the
corresponding R.sup.4 is a hydrogen atom with a C.sub.1-6 alkyl
halide (e.g., methyl iodide, butyl iodide) in the presence of metal
hydride (e.g., NaH). The reaction is preferably carried out at a
temperature from 0.degree. C. to room temperature.
[0184] Procedure (d)
[0185] The compound [I] wherein R.sup.6 is a C.sub.2-6
alkanoylamino-, C.sub.3-7 cycloalkylcarbonylamino-, aryl C.sub.2-7
alkanoylamino-, arylcarbonylamino-, C.sub.1-5
alkyloxycarbonylamino-, C.sub.3-7 cycloalkyloxycarbonylamino-, aryl
C.sub.1-6 alkyloxycarbonylamino-, arylureido, or
arylsulfonylamino-substituted aryl group can be prepared by
reacting the compound [I] wherein the corresponding R.sup.6 is an
amino aryl group or a (C.sub.1-6 alkyl)-amino-substituted aryl
group with a C.sub.2-16 alkanoic acid, an anhydride of C.sub.2-6
alkanoic acid, C.sub.2-6alkanoyl halide, C.sub.3-7
cycloalkcanecarboxylic acid, anhydride of C.sub.3-7
cycloalkane-carboxylic acid, C.sub.3-7 cycloalkanoyl halide, aryl
C.sub.2-7 alkanoic acid, anhydride of aryl C.sub.2-7 alkanoic acid,
aryl C.sub.2-7 alkanoyl halide, arylcarboxylic acid, anhydride of
arylcarboxylic acid, arylcarbonyl halide, C.sub.1-5 alkyl
halogenoformate, arylisocyanate, or arylsulfonyl halide in the
presence or absence of an acid acceptor (e.g., DIEA) and in the
presence or absence of a condensing reagent (e.g., BOP-Cl) which
can be used for a conventional peptide synthesis. The reaction is
preferably carried out at a temperature from one to a room
temperature.
[0186] Procedure (e):
[0187] {circle over (P)}=a resin which is used in a conventional
solid phase peptide synthesis 60
[0188] wherein R.sup.24 is a protecting group for the amino group,
and the other symbols are the same as defined above.
[0189] The compound of the formula [I-1]: 61
[0190] can be prepared by condensing the compound [I] wherein the
corresponding R.sup.2 is a group of the formula: --COOH with a
group of the formula [VIII]: 62
[0191] wherein the symbols are the same as defined above, by a
conventional solid phase peptide synthesis method also known as
Merrifield method (Journal of American Chemical Society 85,
2149-2154 (1963)), followed by the deprotection of amino group and
carboxyl group by a conventional method.
[0192] R.sup.24 can be selected from a conventional protecting
group for an amino group, for example, tert-butoxy-carbonyl grop
(BOC), benzyloxycarbonyl group (Cbz) and the like.
[0193] The solvent used for the Procedures (a) to (e) may be
selected from any one which does not disturb the procedures, for
example, THF, methanol, DMF, CH.sub.2Cl, or a mixture thereof.
[0194] General Description for Synthesis of Intermediates
[0195] The compound [II] may be prepared by reacting a compound of
the formula [VI]: 63
[0196] wherein the symbols are the same as defined above, in the
presence of C.sub.1-6 alkanoyl halide (e.g., AcCl) and/or C.sub.1-6
alkanoic anhydride (e.g., Ac.sub.2O).
[0197] The compound [III-a] may be prepared by a conventional
method, which is selected according to the types of the
substituents, for example, by the following schemes: 646566
[0198] wherein R is (1) a substituted or unsubstituted monocyclic
or bicyclic aryl group or (2) a substituted or unsubstituted
monocyclic or bicyclic heteroaryl group, Hal is a halogen atom and
other symbols are the same as defined above.
[0199] The compound [IV] may be prepared as shown in various
locations of the present application, for example, in Schemes 7, 8,
9, 10 and 11.
[0200] The desired compound [I] of the present invention may also
be prepared by the methods as shown in the following Schemes.
67
[0201] Commercially available (L)-p-nitroPhe-OH (5a-A) (50.6 g,
240.6 mmol) was dissolved in MeOH (250 mL) and dry HCl was bubbled
through the solution for 45 minutes at 0.degree. C. The mixture was
refluxed for 15 minutes and allowed to stand overnight. The HCl
salt precipitated and the solid material was collected by
filtration and washed with Et.sub.2O (3.times.50 mL). The solid
methyl ester (5a-B) thus obtained was pale yellow (55.3 g, 88%):
mp=215-218.degree. C. (d).
[0202] The HCl salt of (L)-p-nitroPhe-OMe (5a-B) (5.2 g, 19.8 mmol)
was dissolved in THF (30 mL) containing DIEA (10.3 mL, 59.4 mmol)
To this solution was added
(1R-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl) ester (15-D) (5.1 g, 19.8 mmol) and BOP
reagent (10.6 g, 23.9 mmol) and the solution was stirred under dry
N.sub.2 for 72 hours. Work-up of the coupling reaction was
performed by the addition of 1N HCl (60 mL) and extraction with
EtOAc (2.times.20 mL). The combined organic phase was washed with
saturated NaHCO.sub.3 (20 mL), then saturated LiCl (15 mL) and
dried over Na.sub.2SO.sub.4. The solution was filtered, solvent
evaporated and the residue chromatographed (SiO.sub.2, gradient
elution: 100% hexanes.fwdarw.50% EtOAc/hexanes) to provide the
fully protected intermediate (1S-cis)-N-[3-{(1,1-dimethyletho-
xy)-2,2,3-trimethylcyclopentyl]carbonyl]-4-nitro-L-phenylalanine
methyl ester (5a-C) (7.1 g, 77%):
[0203] .sup.1H NMR (300 MHz, CDCl.sub.3), .delta. 8.14 (2H), 7.35
(2H). 6.18 (1H), 4.99 (1H), 3.76 (3H), 3.34 (1H), 3.20 (1H), 2.62
(1H), 2.5-2.6 (2H), 2.1-2.2 (1H), 1.6-1.8 (1H), 1.4-1.5 (1H), 1.45
(9H), 1.25 (3H), 1.16 (3H), 0.81 (3H); .sup.13C NMR (75 MHz,
CDCl.sub.3), .delta. 174.57, 172.44, 171.30, 146.69, 144.01,
129.87, 123.31, 79.89, 56.35, 53.98, 52.52, 52.24, 46.10, 37.38,
32.08, 27.72, 22.64, 22.27, 21.62, 20.40; ESMS (m/z) 463
(MH.sup.+).
[0204] The above compound, (5a-C) (2.7 g, 5.77 mmol), was dissolved
in MeOH (40 mL) and degassed with N.sub.2. To this solution was
added 10% Pd-C (250 mg) and H.sub.2 gas was bubbled through the
resultant slurry for 15 minutes and the reaction was stirred an
additional 3 hours under an atmosphere of H.sub.2. The mixture was
filtered through celite and the celite washed with CH.sub.3OH. The
solvent was evaporated to afford Example 56 (5a-D) (2.49 g,
100):
[0205] .sup.1H NMR (300 MHz, CDCl.sub.3), .delta. 6.88 (2H), 6.64
(2H), 5.71 (1H), 4.82 (1H), 3.72 (3H), 3.0-3.1 (2H), 2.5-2.6 (2H),
2.1-2.2 (1H), 1.6-1.8 (1H), 1.4-1.5 (1H), 1.43 (9H) 1.21 (3H), 1.14
(3H), 0.80 (3H); ESMS (m/z) 433 (MH.sup.+). 68
[0206] Commercially available Boc-(L)-Phe(4-N-Cbz)-OH (5b-A) (6.2
g, 14.9 mmol) was dissolved in MeOH (20 mL) and dry HCl was bubbled
through the solution for 10 minutes. This mixture was stirred for 1
hour. The solvent was evaporated and the solid material (5b-B) thus
obtained was washed with cold Et.sub.2O (3.times.20 mL). This solid
material was dissolved in THF (25 mL) containing DIEA (7.8 mL, 44.8
mmol). To this solution was added (15-D) (4.2 g, 16.4 mmol) and BOP
reagent (7.9 g, 17.9 mmol) and the solution was stirred under dry
N.sub.2 overnight. Work-up of the coupling reaction was performed
by the addition of 1N HCl (60 mL) and extraction with EtOAc
(2.times.50 mL). The combined organic phase was washed with
saturated LiCl (35 mL) and dried over Na.sub.2SO.sub.4. The
solution was filtered, solvent evaporated and the residue
chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.500 EtOAc/hexanes) to provide the fully protected
intermediate 4-benzyloxycarbonylamino-N-[[(1S-
,3R)-3-(tert-butoxycarbonyl)-2,2,3-trimethylcyclopentyl]carbonyl]-L-phenyl-
alanine methyl ester (5b-C) (6.5 g, 77%) .sup.1H NMR (300 MHz,
CDCl.sub.3), .delta. 7.3-7.4 (6H), 7.17 (1H), 7.01 (2H), 5.80 (1H),
5.17 (2H), 4.86 (1H), 3.70 (3H) 3.06 (2H), 2.5-2.6 (2H) 2.1-2.2
(1H), 1.6-1.8 (1H), 1.4-1.5 (1H), 1.43 (9H) 1.21 (3H), 1.13 (3H),
0.80 (3H); .sup.13C NMR (75 MHz, CDCl.sub.3) .delta. 174.88,
172.43, 172.06, 153.32, 136.98, 135.95, 130.55, 129.58, 128.46,
128.19, 128.15, 118.71, 80.05, 66.80, 56.54, 54.26, 52.98, 52.17,
46.25, 36.94, 32.21, 27.92, 22.81, 22.32, 21.82, 20.46; ESMS (m/z)
567 (MH.sup.+).
[0207] The above compound, (5b-C) (5.74 g, 10.13 mmol), was
dissolved in MeOH/THF (4:1, 50 mL) and degassed with N.sub.2. To
this solution was added 10% Pd-C (500 mg) and H.sub.2 gas was
bubbled through the resultant slurry for 1 hour. The reaction was
stirred an additional 3 hours under an atmosphere of H.sub.2. The
mixture was filtered though celite and the celite washed with
CH.sub.3OH. The solvent was evaporated to afford Example 56 (5a-D)
(4.38 g, 100%):
[0208] .sup.1H NMR (300 MHz, CDCl.sub.3), .delta. 6.90 (2H), 6.68
(2H), 5.73 (1H), 4.82 (1H), 3.72 (3H), 3.0-3.1 (2H), 2.5-2.6 (2H),
2.1-2.2 (1H), 1.6-1.8 (1H), 1.4-1.5 (1H), 1.43 (9H), 1.21 (3H),
1.14 (3H), 0.80 (3H); ESMS (m/z) 433 (MH.sup.+). 69
[0209] (1R)-Camphoric anhydride (243 mg, 1.33 mmol) was dissolved
in THF (10 mL) containing DIEA (1.2 mL, 6.67 mmol). To this
solution O-2,6-dichlorobenzyl-L-tyrosine methyl ester (6-A) (618
mg, 1.58 mmol) was added and the solution stirred at 45.degree. C.
for 1 h. The reaction was cooled to room temperature and 1 N HCl
(20 mL) was added. This was extracted with EtOAc (2.times.20 mL)
and the combined organics were dried (Na.sub.2SO.sub.4), filtered,
and the solvent removed in vacuo. The residue was chromatographed
(SiO.sub.2, 10% MeOH in CH.sub.2Cl.sub.2) to provide (6-B) (668 mg,
93%) as a colorless oil: .sup.1H NMR (300 MHz, CDCl.sub.3), (major
isomer) .delta. 7.34 (d, 2H), 7.23 (dd, 1H), 7.04 (d, 2H), 6.9-7.0
(m, 2H), 5.85 (d, 1H), 5.23 (s, 2H), 4.88 (q, 1H), 3.73 (s, 3H),
3.0-3.2 (m, 2H), 2.5-2.6 (m, 2H), 2.2-2.3 (m, 1H), 1.7-1.8 (m, 1H),
1.4-1.5 (m, 1H), 1.23 (s, 3H), 1.22 (s, 3H), 0.84 (s, 3H); ESMS
(m/z) 536 (MH.sup.+).
[0210] (6-B) (570 mg, 1.06 mmol) was dissolved in THF (2 mL). To
this solution LiOH (89 mg, 3.72 mmol) was added in H.sub.2O (2 mL)
and the mixture stirred for 12 h at RT. The reaction was acidified
with 1 N HCl (10 mL) and then extracted with EtOAc (2.times.20 mL).
The combined organics were dried (Na.sub.2SO.sub.4), filtered, and
the solvent removed in vacuo to provide Example 12 (6-C) (525 mg,
95%) as a pale yellow foam: .sup.1H NMR (300 MHz, Acetone-d.sub.6),
(major isomer) .delta. 7.4-7.5 (m, 3H), 7.23 (d, 2H), 6.99 (d, 2H),
5.30 (d, 1H), 5.28 (s, 2H), 4.6-4.7 (m, 1H), 3.13 (dd, 1H), 2.97
(dd, 1H), 2.81 (t, 1H), 2.5-2.6 (m, 1H), 2.0-2.1 (m, 1H), 1.6-1.7
(m, 1H), 1.3-1.4 (m, 1H), 1.27 (s, 3H), 1.19 (s, 3H), 0.80 (s, 3H);
.sup.13C NMR (75 MHz, Acetone-d.sub.6), (major isomer) .delta.
177.79, 173.99, 172.94, 158.53, 137.39, 133.18, 132.01, 131.23,
129.52, 115.24, 65.78, 56.75, 54.47, 53.67, 46.91, 37.19, 33.30,
23.44, 23.01, 22.38, 21.64; ESMS (m/z) 520 (M-H).sup.-. 70
Preparation 7-A
Scheme 7, 7-A Stereochemistry=1S-cis (Intermediate for Examples
181, 185, and 188)
[0211] (1R,3S)-Camphoric Acid 1-(1,1-Dimethylethyl)-3-phenylmethyl
ester (C.sub.21H.sub.30O.sub.4)
[0212] Benzyl bromide is eluted through neutral alumina (10 mL in a
30 mL sintered glass funnel) to give a colorless liquid (15 mL, 126
mmol) which is added to a stirred solution of (1R,3S)-camphoric
acid 1-(1,1-dimethylethyl) ester (15-D) (30 g, 117 mmol),
N,N-diisopropylethyl amine (24 mL, 138 mmol), and acetonitrile (90
ml). After seven days, the mixture is filtered to give a white
solid (diisopropylethyl amine hydrobromide) and a yellow liquid
which is placed in the freezer. After two days, the mixture is
filtered (two 50 mL diethyl ether rinses) to give a white solid (24
g, 59% yield).
[0213] .sup.1H NMR: (300 MHz, CDCl.sub.3): .delta. 7.37-7.29 (5H),
5.15 (1H), 5.09 (1H), 2.85-2.79 (1H), 2.54-2.46 (1H), 2.24-2.15
(1H), 1.86-1.74 (1H), 1.49-1.36 (1H), 1.43 (9H), 1.23 (,3H), 1.15
(3H), 0.78 (3H); IR (nujol) 1737, 1724, 1717, 1346, 1272, 1259,
1219, 1210, 1162, 1124, 1116, 1084, 852, 737, 696 cm.sup.-1; MS
(FAB) m/z (rel. intensity) 347 (M+H, 35), 348 (8), 347 (35), 292
(8), 291 (43), 273 (12), 109 (13), 92 (9), 91 (99), 57 (30), 41
(9); HRMS (FAB) calcd for C.sub.21H.sub.30O.sub.4+H.sup.+ 347.2222,
found 347.2232; Anal. Calcd for C.sub.21H.sub.30O.sub.4: C, 72.80;
H, 8.73; Found: C, 72.79; H, 8.90.
[0214] (1R,3S)-Camphoric Acid 3-phenylmethyl ester (7-A)
(C.sub.17H.sub.22O.sub.4)
[0215] To (1R,3S)-camphoric Acid
1-(1,1-Dimethylethyl)-3-phenylmethyl ester (24 g, 69 mmol) is added
trifluoroacetic acid (15 mL). After stirring for two days, the
solution is evaporated in vacuo to give a pale yellow oil which is
dissolved in toluene (250 mL) and shaken with water (6.times.100
mL). Evaporation of the toluene gave a colorless oil which slowly
crystallizes to give 7-A as an oily, white solid (16.4 g, 81%
yield).
[0216] .sup.1H-NMR: (300 MHz, CDCl.sub.3) .delta. 7.38-7.16 (5H),
5.17 (1H), 5.11 (1H), 2.87 (1H), 2.60-2.49 (1H) 2.30-2.21 (1H),
1.91-1.80 (1H) 11.57-1.48 (1H), 1.27 (3H), 1.25 (3H), 0.84 (3H); IR
(liq.) 3067, 3034, 2972, 2888, 1732, 1696, 1457, 1378, 1285, 1231,
1212, 1166, 1124, 752, 698 cm.sup.-1; MS (FAB) m/z (rel. intensity)
291 (M+H, 44), 391 (5), 292 (8), 291 (44), 273 (6), 245 (4), 155
(3), 109 (10), 92 (9), 91 (99), 41 (3); HRMS (FAB) m/z calcd for
C.sub.17H.sub.22O.sub.4+H.sup.+ 291.1596, found 291.1603; Anal.
Calcd for C.sub.17H.sub.22O.sub.4: C, 70.32; H, 7.64; Found: C,
70.21; H, 7.89.
Preparation 7-C-1
Scheme 7, 7-C: wherein R.sup.7-1=H Scereochemistry=1S-cis
[0217]
(1R-cis)-N-[[3-[(phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentyl-
]carbonyl]glycine (1,1-dimethylethyl) ester (7-C-1)
(C.sub.23H.sub.33NO.sub.5)
[0218] To (1R,3S)camphoric acid 3-phenylmethyl ester 7-A (0.736 g,
2.53 mm) in dry DMF (5 mL) is added diisopropylethyl amine (3 mL,
17.2 mmol) and HATU (1.05 g, 2.76 mmol). Thirty minutes later, 7-B
(R.sup.7-1=H) hydrochloride (0.855 g, 5.10 mm) is added. After
overnight stirring, the mixture is evaporated to dryness (in
vacuo/N.sub.2 flow) and then mixed with toluene (50 mL) and THF (50
mL) and washed with water (2.times.50 mL), 1N HCl (50 mL), and
water (4.times.50 mL). The organic layer is then evaporated to
dryness, giving 7-C(R.sup.7-1=H) as an off-white solid (0.9 g,
90%)
[0219] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.37-7.15 (5H)
6.05 (1H), 5.17-5.08 (2H), 3.99-3.82 (2H), 2.89-2.83 (1H),
2.51-2.40 (1H), 2.29-2.22 (1H), 1.94-1.80 (1H), 1.59-1.51 (1H),
1.47 (9H), 1.31 (3H), 1.21 (3H), 0.78 (3H). MS (ES+) m/z 404.1
(parent).
Preparation 7-D
Scheme 7, 7-D: wherein R.sup.7-1=H Stereochemistry=1S-cis
[0220]
(1R-cis)-N-[[3-carboxy-1,2,2-trimethylcyclopentyl]carbonyl]glycine
(1,1-dimethylethyl) ester (7-D-1) (C.sub.16H.sub.27NO.sub.5)
[0221] Ester 7-C (R.sup.7-1=H, 0.97 g, 2.4 mmol) in THF (12 mL) and
ethanol (6 mL) is shaken with 10% Pd/C (0.115 g) in a Parr bottle
under an H.sub.2 (38 psi) atmosphere. After 8 hours, the bottle is
removed from the shaker and filtered through Celite (with
3.times.30 mL ethanol rinses). The filtrate is evaporated to
dryness, giving 7-D (R.sup.7-1=H) as a thick, colorless oil (0.6 g,
79% yield).
[0222] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 6.13 (1H),
4.0-3.84 (2H) 2.86-2.80 (1H), 2.49-2.39 (1H), 2.27-2.17 (1H),
1.94-1.80 (1H), 1.60-1.52 (1H), 1.46 (9H), 1.34 (3H), 1.22 (3H),
0.88 (3H); IR (liq.) 3385, 2976, 2940, 2887, 1732, 1644, 1528,
1478, 1459, 1405, 1394, 1369, 1277, 1227, 1158 cm.sup.-1; MS (FAB)
m/z (rel. intensity) 314 (M+H, 99), 315 (18), 314 (99), 258 (51),
109 (22), 95 (9), 76 (20), 69 (12), 57 (28), 55 (12), 41 (14). HRMS
(FAB) m/z calcd for C.sub.16H.sub.27NO.sub.5+H1 314.1967, found
314.1974; Anal. Calcd for C.sub.16H.sub.27NO.sub.5 C, 61.32; H,
8.68; N, 4.47; Found: C, 61.70; H, 8.86; N, 4.14; Melt Solvate:
3.906 Ethanol.
Preparation 7-F-1
Scheme 7, 7-F: wherein R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl
Stereochemistry=(1S-cis)-L
[0223]
(1S-cis)-N-[[3-[[(1,1-dimethylethoxycarbonylmethyl)amino]carbonyl]--
2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phen-
ylalanine methyl ester (7-F-1)
(C.sub.33H.sub.41Cl.sub.2N.sub.3O.sub.7)
[0224]
(1R-cis)-N-[[3-carboxy-1,2,2-trimethylcyclopentyl]carbonyl]glycine
(1,1-dimethylethyl) ester 7-D (R.sup.7-1=H, 0.356 g, 1.14 mmol) is
dissolved in methylene chloride (6 mL), under N.sub.2 in a round
bottom flask, and is cooled in an ice water bath. To this stirred
solution is added N,N-diisopropylethylamine (1 mL, 5.7 mmol), EDC
(0.242 g, 1.26 mmol), HOBt (0.181 g, 1.34 mmol), and
4-N,N-dimethylaminopyridine (0.016 g, 0.13 mmol) followed 30
minutes later by 4-[(2,6-dichlorobenzoyl)amino]- -L-phenylalanine
methyl ester hydrochloride 7-E-1.HCl (7E: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]phenyl, Stereochemistry=S)
(0.487 g, 1.2 mmol). After two days of stirring (the ice bath is
allowed to melt), the reaction mixture is evaporated to dryness and
then partitioned between THF (100 mL), diethyl ether (50 mL), and
water (50 mL). The organic layer is washed with water (3.times.50
mL), aqueous HCl (0.5N, cold, 3.times.30 mL), aqueous sodium
bicarbonate (1.times.50 mL), water (3.times.30 mL, to pH 7), and
then evaporated to dryness, giving 7-F (R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
[0225] Stereochemistry=(1S-cis)-L) as a white solid (0.63 g, 80%
yield).
[0226] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.57 (2H),
7.38-7.26 (3H) 7.10 (2H), 6.07 (1H), 5.80 (1H), 4.89 (1H),
3.97-3.74 (2H), 3.21-3.05 (2H), 2.58-2.52 (1H), 2.45-2.35 (1H),
2.30-2.18 (1H), 1.90-1.75 (1H), 1.58-1.50 (1H), 1.46 (9H), 1.30
(3H), 1.20 (3H), 0.79 (3H); IR (nujol) 1739, 1668, 1643, 1609,
1560, 1538, 1516, 1431, 1414, 1327, 1288, 1256, 1235, 1195, 1161
cm.sup.-1; MS (FAB) m/z (rel. intensity) 662 (M+H, 84), 664 (58),
663 (39), 662 (84), 533 (39), 531 (55), 240 (58), 194 (37), 173
(41), 109 (99), 57 (44); HRMS (FA3) m/z calcd for
C.sub.33H.sub.41Cl.sub.- 2N.sub.3O.sub.7+H.sup.+ 662.2399, found
662.2410.
Preparation 7-G-1
Scheme 7, 7-G: wherein R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl
Stereochemistry=(1S-cis)-L
[0227]
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcycl-
opentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (7-G-1) (C.sub.29H.sub.33Cl.sub.2N.sub.3O.sub.7)
[0228]
(1S-cis)-N-[[3-[[(1,1-dimethylethyloxycarbonylmethyl)damino]carbony-
l]-2,2,3-trimethylcyclopentyl]carbonyl-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine methyl ester 7-F (R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl,
Stereochemistry=(1S-cis)-L, 0.802 g, 1.21 mmol) is stirred
overnight in trifluoroacetic acid (3 mL). The solution is then
diluted with toluene (5 mL) and evaporated to dryness in vacuo to
give an off white solid which was recrystallized from
chloroform/diethyl ether to give 7-G (R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl),amino]-phenyl, Stereochemistry
(1S-cis)-L as a white solid (0.7 g, 90% yield).
[0229] .sup.1H-NMR: (300 MHz, DMSO-d.sub.6): .delta. 10.68 (1H),
7.92 (2H), 7.58-7.44 (3H), 7.19 (2H), 4.53-4.45 (1H), 3.75-3.62
(2H), 3.58 (3H), 3.03-2.85 (2H), 2.68-2.62 (1H), 2.40-2.28 (1H),
1.98-1.81 (1H), 1.70-1.50 (1H), 1.30-1.25 (1H), 1.17 (3H), 1.09
(3H).
Preparation of Example 181
Scheme 7, 7-G: wherein R.sup.7=H R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
Stereochemistry=(1S-cis)-L
[0230]
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcycl-
opentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(Example 181) (C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7)
[0231]
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcycl-
opentenyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester. (7-G-1) (0.7 g, 1.15 mmol) is dissolved in methanol
(12 mL). To this is added a mixture of LiOH H.sub.2O (0.243 g, 5.8
mmol), aqueous H.sub.2O.sub.2 (30%, 2 mL), and H.sub.2O (2 mL).
After overnight stirring, the reaction mixture is diluted with
water (50 mL), and evaporated (room temperature, in vacuo/N.sub.2
flow) until the methanol is gone. The aqueous solution is then
transferred to a separatory funnel and shaken with diethyl ether
(2.times.20 mL). The aqueous layer is then evaporated, to remove
residual diethyl ether, and cooled in an ice water bath. The
stirred solution is then brought to pH 3-4 using aqueous HCl (1N).
The resultant precipitate is isolated by suction filtration (with
water washes) to give Example 181 as a white solid 0.4 g, 58%
yield)
[0232] .sup.1H-NMR: (300 MHz, DMSO-d.sub.6): 612.45 (1H), 10.6 (1H)
7.74 (2H), 7.57-7.44 (3H), 7.20 (2H), 4.48-4.40 (1H), 3.65 (2H),
2.94 (2H), 2.64 (1H), 2.35 (1H), 1.90 (1H), 1.58 (1H), 1.29 (1H),
1.18 (3H), 1.08 (3H), 0.60 (3H); IR (nujol) 3124, 3088, 3078, 1738,
1666, 1628, 1612, 1588, 1563, 1552, 1521, 1429, 1334, 1197, 1170
cm.sup.-1; MS (FAB) m/z (rel. intensity) 592 (M+H, 99), 595 (20),
594 (69), 593 (41), 592 (99), 519 (25), 517 (38), 240 (55), 175
(23), 173 (33), 109 (64); HRMS (FAB) m/z calcd for
C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7+H.sup.+ 592.1617, found
592.1606; Anal. Calcd for C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7:
C, 56.76; H, 5.27; N, 7.09; Found: C, 54.92; H, 5.41; N, 6.91; KF
Water: 3.05% H.sub.2O.
Preparation 7F-2
Scheme 7, 7-F: wherein wherein R.sup.7-1=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]-phenyl
Stereochemistry=(1S-cis)-L
[0233]
[1S-cis]-O-[((2,6-Dichlorophenyl)methyl)]-N-[[3-[[(1,1-diemethyleth-
oxy)carbonylmethyl)amino]-carbonyl]-2,2,3-trimethylcyclopentyl)carbonyl]-L-
-tyrosine methyl ester (7-F-2)
(C.sub.33H.sub.42Cl.sub.2N.sub.2O.sub.7) is prepared from 7-D
(R.sup.7-1=H and O-[(2,6-dichlorophenyl)methyl]-L-tyros- ine methyl
ester 7E-2 (7-E: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl, Stereochemistry=S)
as taught by Scheme 7.
[0234] .sup.1H NMR (CDCl.sub.3) .delta. 7.39-6.94 (7H), 6.09 (1H),
5.78 (1H), 5.25 (2H), 4.87 (1H), 3.90 (2H), 3.74 (3H), 3.09 (2H),
2.59-2.20 (3H), 1.80 (1H), 1.56 (1H), 1.47 (9H), 1.30 (3H), 1.26
(3H), 0.81 (3H); IR (mull) 3327, 1762, 1741, 1664, 1637, 1538,
1512, 1440, 1241, 1229, 1206, 1198, 1174, 1156, 1022 cm.sup.-1; MS
(FAB) m/z (rel. intensity) 649 (M+H, 50), 651 (34), 649 (50), 518
(21), 296 (23), 240 (44), 194 (28), 161 (26), 159 (41), 109 (99),
57 (37)
Preparation 7-G-2
Scheme 7, 7-G: wherein R.sup.7-1=H, R.sup.4=H,
R.sup.1=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl
Stereochemistry=(1S-cis)-L
[0235]
[1S-cis]-O-[((2,6-Dichlorophenyl)methyl)]-N-[[3-[[(Carboxymethyl)am-
ino]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-L-tyrosine
methyl ester (7-G-2) (C.sub.29H.sub.34Cl.sub.2N.sub.2O.sub.7) is
prepared from 7-P-2 as taught by Scheme 7.
[0236] .sup.1H NMR (CDCl.sub.3) .delta. 8.14 (1H), 7.37-6.93 (7H),
6.51 (1H), 6.02 (1H), 5.24 (2H), 4.85 (1H), 4.02 (2H), 3.73 (3H),
3.09 (2H), 2.57 (1H), 2.41 (1H), 2.25 (1H), 1.84 (1H), 1.56 (1H),
1.26 (3H), 1.20 (3H), 0.79 (3H); MS (ES+) m/z 592.9.
Preparation of Example 185
Scheme 7, 7-G: wherein R.sup.7-1=H, R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl,
Stereochemistry=(1S-cis)-L
[0237]
(1S-cis)-N-[[3-[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine (Example
185) (C.sub.28H.sub.32Cl.sub.2N.sub.2O.sub.7) is prepared from
7-G-2 (R.sup.7-1=H, R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]-phenyl, Stereochemistry=S)
as taught by Scheme 7.
[0238] .sup.1H NMR (300 MHz, DMSO-d.sub.6) 7.8-6.9 (9H), 5.18 (2H),
4.42 (1H), 3.8-3.6 (2H), 3.02-2.82 (2H), 2.65 (1H), 2.38 (1H), 1.91
(1H), 1.58 (11H), 1.30 (11H), 1.19 (3H), 1.10 (3H), 0.61 (3H); IR
(nujol) 3409, 1733, 1645, 1612, 1585, 1564, 1511, 1439, 1297, 1239,
1197, 1179, 1018, 786, 770 cm.sup.-1; MS (FAB) m/z (rel. intensity)
579 (M+H, 99), 582 (22), 581 (67), 580 (44), 579 (99), 578 (21),
240 (34), 161 (21), 159 (34), 109 (46), 91 (37); HRMS (FAB) m/z
calcd for C.sub.28H.sub.32Cl.sub.- 2N.sub.2O.sub.7+H.sup.+
579.1664, found 579.1667.
Preparation 7-C-2
Scheme 7, 7-C: wherein R.sup.7-1=CH.sub.3
Stereochemistry=[1S-[1.alpha.,3.- alpha.(R*)]]
[0239]
[1S-[1.alpha.,3.alpha.(R*)]]-3-[[[1-(1,1-Dimethylethoxycarbonyl)eth-
yl]amino]carbonyl]-2,2,3-trimethylcyclopentanecarboxylic acid
[phenyl(methyl)] ester (7-C-2) (C.sub.24H.sub.35NO.sub.5) is
prepared from 7-A and 7-B. (R.sup.7-1=CH.sub.3, Stereochemistry=s)
as taught by Scheme 7.
[0240] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.37-7.16 (5H),
6.18 (1H), 5.12 (2H), 4.41 (1H), 2.84 (1H), 2.46 (1H), 2.28 (1H),
1.87 (1H), 1.52 (1H), 1.51 (9H), 1.35 (3H), 1.28 (3H), 1.20 (3H),
0.78 (3H). MS (ES+) m/z 455.1
Preparation 7-D-2
Scheme 7, 7-D: wherein wherein R.sup.7-1=CH.sub.3
Stereochemistry=[1S-[1.a- lpha.,3.alpha.(R*)]]
[0241]
[1S-[1.alpha.,3.alpha.(R*)]]-3-[[[1-(1,1-Dimethylethoxycarbonyl)eth-
yl]amino]carbonyl]-2,2,3-trimethylcyclopentanecarboxylic acid
(7-D-2) (C.sub.17H.sub.29NO.sub.5) is prepared from 7-C-2 as taught
by Scheme 7.
[0242] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 6.28 (1H), 4.44
(1H), 2.86 (1H) 2.43 (1H), 2.23 (1H), 1.93 (1H), 1.56 (1H), 1.47
(9H), 1.36 (3H), 1.31 (3H), 1.22 (3H), 0.90 (3H); MS (ES-) m/z
326.1.
Preparation 7-F-3
Scheme 7, 7-F: wherein R.sup.7-1=CH.sub.3, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl
Stereochemistry=[1S-[1.alpha.,3.alpha.(R*)]]
[0243]
[1S-[1.alpha.,3.alpha.(R*)]]-O-[(2,6-Dichlorophenyl)methyl]-N-[[3-[-
[[1-(1,1-dimethylethoxycarbonyl)ethyl]amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-L-tyrosine methyl ester (7-F-3)
(C.sub.34H.sub.44Cl.sub.2- N.sub.2O.sub.7) is prepared from 7-D-2
and 7-E-2 (7E: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl) as taught by Scheme
7.
[0244] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.38-6.93 (7H),
6.20 (1H), 5.77 (1H), 5.25 (2H), 4.87 (1H), 4.42 (1H), 3.73 (3H),
3.09 (2H), 2.44 (2H), 1.76 (2H), 1.52 (1H), 1.46 (9H), 1.35 (3H),
1.27 (3H), 1.20 (3H), 0.80 (3H). IR (nujol) 1739, 1654, 1612, 1585,
1565, 1511, 1439, 1344, 1300, 1240, 1198, 1177, 1154, 1017, 768
cm.sup.-1. MS (FAB) m/z (rel. intensity) 663 (M+H, 82), 665 (60),
664 (37), 663 (82), 518 (27), 254 (51), 208 (24), 161 (28), 159
(45), 109 (99), 57 (33).
Preparation 7-G-3
Scheme 7, 7-G: wherein R.sup.7-1=CH.sub.3, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl
Stereochemistry=[-[1.alpha.,3.alpha.(R*)-L
[0245]
[1S-[1.alpha.,3.alpha.(R*)]]-O-[(2,6-Dichlorophenyl)methyl]-N-[[3-[-
[(1-Carboxyethyl)amino]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-L-ty-
rosine methyl ester (7-G-3)
(C.sub.30H.sub.36Cl.sub.2N.sub.2O.sub.7) is prepared from 7F-3 as
taught by Scheme 7.
[0246] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 10.55 (1H),
7.38-6.94 (7H) 6.47 (1H), 6.12 (1H), 5.25 (2H), 4.87 (2H), 4.55
(1H), 3.75 (3H), 3.10 (2H), 2.60 (1H), 2.41 (1H), 2.25 (1H), 1.87
(1H), 1.58 (1H), 1.46 (3H), 1.24 (3H), 1.21 (3H), 0.78 (3H); MS
(ES+) m/z 606.8.
Preparation of Example 188
Scheme 7, 7-G: wherein R.sup.7-1=CH.sub.3, R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl,
Stereochemistry=[1S-[1.alp- ha.,3.alpha.(R*)]
[0247]
[1S-[1.alpha.,3.alpha.(R)]]-N-[[3-[(1-Carboxyethyl)amino]carbonyl]--
2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,6-dichlorophenyl)
methyl]L-tyrosine (C.sub.29H.sub.34Cl.sub.2N.sub.2O.sub.7) is
prepared from 7-G-3 as taught by Scheme 7.
[0248] .sup.1H NMR (300 MHz, DMSO-d.sub.6 7.71 (1H), 7.54-7.43
(3H), 7.28 (1H), 7.16 (2H), 6.93 (2H), 5.16 (2H), 4.40 (1H), 4.16
(1H), 3.02-2.80 (2H), 2.63 (1H), 2.35 (1H), 1.86 (1H), 1.54 (1H),
1.35-1.23 (4H), 1,14 (3H), 1.08 (3H), 0.59 (3H); IR (nujol) 3427,
3031, 1731, 1645, 1612, 1585, 1565, 1512, 1439, 1297, 1239, 1230,
1197, 1179, 1017 cm.sup.-1; MS (FAB) m/z (rel. intensity) 593 (M+H,
99), 596 (22), 595 (69), 594 (43), 593 (99), 592 (17), 504 (22),
254 (63), 161 (44), 159 (40), 109 (72). 71
Preparation 8-C-1
Scheme 8, 8-C: wherein: R.sup.8-1=H, R.sup.8-2H
Stereochemistry=1S-cis
(1S-cis)-N-[1-[(phenylmethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbon-
yl]glycine amide (8-C-1) (C.sub.19H.sub.26N.sub.2O.sub.4)
[0249] A solution of
(1R-cis)-[3-(phenylmethoxy)carbonyl]-1,2,2-trimethylc-
yclopentanecarboxylic acid (7-A) (1 g, 3.44 mmol) in dry DMF is
cooled in an ice water bath and diisopropylethylamine (1.77 g,
13.76 mmol, 2.39 mL), HATU (1.35 g, 3.55 mmol), and 8-B
(R.sup.8-1=H, R.sup.8-2=H) (0.38 g, 3.44 mmol) are added in order.
The mixture is allowed to stir for 48 hours as the ice melts and
the solution warms to room temperature The solution is cast into
methylene chloride (0.25L) and 1N aq. NaOH (0.25L). The organic
phase is separated and washed in order with 1N aq. HCl (0.25L),
water (5.times.0.25L), and brine (0.25L). The organic phase is
dried and concentrated in vacuo to give the crude amide as an ivory
powder. The crude amide is recrystallized from hexanes-chloroform
to furnish the target amide 8-C (R.sup.8-1=H, R.sup.8-2=H) as a
fine, free flowing, white powder.
[0250] MP: 163-164.degree. C.; .sup.1H-NMR (300 MHz, CDCl.sub.3):
.delta. 7.25-7.40 (5H) 6.36 (1H), 6.08 (1H), 5.33 (3H), 5.15 (1H),
5.10 (1H), 3.93 (2H), 2.85 (1H), 2.43 (1H), 2.27 (1H), 1.40-1.65
(2H), 1.30 (3H), 1.21 (3H), 0.76 (3H); IR (nujol) 3383, 3361, 3184,
2924, 1766, 1710, 1684, 1626, 1527, 1402, 1253, 1166, 753
cm.sup.-1; MS (EI) m/z (rel intensity) 346 (M.sup.+, 4), 329 (2),
273 (3), 255 (3), 239 (3), 211 (6), 153 (11), 109 (17), 91 (base);
Anal. calcd for C.sub.19H.sub.26N.sub.2O.s- ub.4: C, 65.88; H,
7.56; N, 8.09; Found: C, 65.94, H, 7.65; N, 8.09.
Preparation 8-D-1
Scheme 8, 8-D: wherein: R.sup.8-1=H, R.sup.8-2=H
Stereochemistry=1S-cis
[0251]
(1R-cis)-N-[[3-carboxy-1,2,2-trimethylcyclopentyl]-carbonyl]glycine
amide (8-D-1) (C.sub.12H.sub.20N.sub.2O.sub.4).
[0252] A solution of 8-C (R.sup.8-1=H, R.sup.8-2=H) (3.46 g, 10
mmol) in THF (225 mL), containing 10% Pd/C (1.14 g), is
hydrogenated under 50 psi of hydrogen for 12 hours. The catalyst is
removed by filtration through a cake of Celite, the filter cake is
rinsed with THF (100 mL), and the combined filtrates are
concentrated in vacuo to give the crude product as a white foam.
The crude material is recrystallized from hexanes-THF to afford the
target compound 8-D (R.sup.8-1=H, R.sup.8-2=H) (2.4 g, 94%) as fine
white needles.
[0253] MP: 86-87.degree. C.; .sup.1H-NMR (300 MHz, DMSO-d.sub.6):
.delta. 12.00 (1H) 7.28 (1H), 7.13 (1H), 6.92 (1H), 3.56 (2H), 2.67
(1H), 2.34 (1H), 1.97 (1H) 1.72 (1H), 1.36 (1H), 1.19 (3H), 1.09
(3H), 0.68 (3H); IR (nujol) 3496, 3391, 3189, 2924, 1729, 1705,
1686, 1623, 1519, 1401, 1280, 1245, 1200, 665 cm.sup.-1; MS (EI)
m/z (rel intensity) 238 (2), 221 (6), 195 (base) 138 (26), 109
(81), 95 (67); Anal. calcd for C.sub.12H.sub.2N.sub.2O.sub.4: C,
56.24; H, 7.86; N, 10.93; Found: C, 55.90; H, 8.05; N, 10.50.
Preparation 8-F-1
Scheme 8, 8-F: wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
Stereochemistry=(1S-cis)-L
[0254]
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethy-
lcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (C.sub.29H.sub.34Cl.sub.2N.sub.4O.sub.6)
[0255] To 8-D (R.sup.8-1=H, R.sup.8-2=H)) (1.03 g, 4 mmol) in dry
DMF (25 mL), cooled in a an ice-water bath, is added in order,
diisopropylethylamine (2.07 g, 16 mmol, 2.8 mL),
4-(2,6-dichlorobenzamido- )-L-phenylalanine methyl ester
hydrochloride 7-E-1.HCl (7-E: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]phenyl) (1.48 g, 4 mmol), and
HATU (1.49 g, 4.2 mmol). The mixture is stirred for 48 hours as the
ice melts and the mixture warms to room temperature. The solution
is cast into ethyl acetate (1L) and this solution is washed
successively with 1N aq. HCl (1L), 1N aq. NaOH (1L), water
(4.times.1L), and brine (1L). The organic phase is seperated, dried
(Na.sub.2SO.sub.4), and concentrated in vacuo to give the desired
product 8-F (R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=C.sub.2CH.sub.3, R.sup.6=4-[(2,6-dichloro-
benzoyl)amino]phenyl) (1.12 g, 42%) as a fine, white powder.
[0256] MP: 232-233.degree. C.; .sup.1H-NMR (300 MHz, DMSO-d.sub.6):
.delta. 10.75 (1H), 7.94 (1H), 7.45-7.70 (5H), 7.35 (1H), 7.19
(2H), 7.13 (1H), 6.92 (1H), 4.81 (1H), 3.57 (3H), 3.55 (2H), 2.92
(2H), 2.64 (1H), 2.34 (1H), 1.88 (1H), 1.62 (1H), 1.30 (1H), 1.16
(3H), 1.07 (3H), 0.58 (3H); IR (nujol): 3344, 3251, 3194, 3126,
3072, 2924, 1743, 1699, 1669, 1652, 1623, 1528, 1432, 1328, 799
cm.sup.-1; MS (FAB): m/z (rel. intensity) 605 (M+2H), base), 531
(28), 503 (2), 367 (7), 349 (17), 256 (10), 239 (66), 194 (31), 173
(37), 137 (12), 109 (83); Anal. calcd for
C.sub.29H.sub.34Cl.sub.2N.sub.4O.sub.6.0.3H.sub.2O: C, 57.02; H,
5.71; N, 9.17; Found: C, 57.01; H, 5.86; N, 8.89.
Preparation of Example 180
Scheme 8, 8-G: wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
Stereochemistry=(1S-cis)-L
[0257]
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]-carbonyl]-2,2,3-trimeth-
ylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(Example 180) (C.sub.28H.sub.32Cl.sub.2N.sub.4O.sub.6)
[0258] To 8-F (R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3, R.sup.6
4-[(2,6-dichlorobenzoyl)amino]-phenyl) (1.05 g, 1.7 mmol),
dissolved in methanol (30 mL), is added a solution of
LiOH.sub.2H.sub.2O (0.32 g, 7.65 mmol) in water (10 mL), dropwise
over 15 minutes. The mixture stirs for 18 hours at room temperature
and the pH is then adjusted to ca. 7 by the careful addition of 1N
aq. HCl. The majority of the methanol, is removed in vacuo and the
pH of the resulting solution is adjusted to ca. 2 with 1N aq. HCl.
The resulting flocculent white precipitate is isolated by
filtration and dried. The solid is crushed and washed with water
(2.times.10 mL) and dried in vacuo at 50.degree. C. to give 0.97 g
(97%) of 8-G (R.sup.8-1=H, R.sup.8-2, R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]-phen- yl) as a white,
powdery solid.
[0259] MP: 203-205.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6):
12.51 (1H) 10.70 (1H), 7.75 (1H), 7.45-7.57 (3H), 7.33 (1H), 7.20
(2H), 7.11 (1H), 6.92 (1H), 4.43 (1H), 3.63 (1H), 3.47 (2H), 3.30
(2H), 3.01 (1H), 2.84 (1H), 2.31 (1H), 1.87 (1H), 1.55 (1H), 1.31
(1H), 1.17 (3H), 1.08 (3H), 0.59 (3H); IR (nujoll): 3511, 3325,
3128, 3082, 2868, 1722, 1697, 1664, 1614, 1555, 1537, 1417, 1337,
1246, 799 cm.sup.-1; MS (FAB) m/z (rel. intensity) 591 (M+H, base),
517 (32), 335 (26), 239 (32), 173 (39), 109 (63), 57 (80); HRMS
(FAB) m/z calcd for C.sub.28H.sub.32Cl.sub.2N.sub-
.4O.sub.6+H.sup.+ 591.1777, found 591.1747.
Preparation 8-F-2
Scheme 8, 8-F: wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.31
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl
Stereochemistry=(1S-cis)-L
[0260]
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethy-
lcyclopentyl]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
methyl ester (8-F-2) (C.sub.29H.sub.35Cl.sub.2N.sub.3O.sub.6) is
prepared from 8-D (R.sup.8-1=H, R.sup.8-2=H) and 7-E-2 (R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl) as taught by Scheme
8.
[0261] .sup.1H NMR (CDCl.sub.3) .delta. 7.37-6.93 (7H), 6.54 (1H),
6.40 (TH), 5.82 (1H), 5.60 (1H), 524 (2H), 4.85 (1H), 3.93 (2H),
3.73 (3H), 3.09 (2H), 2.54 (1H), 2.40 (1H), 2.23 (1H), 1.78 (1H),
1.52 (1H), 1.27 (3H), 1.20 (3H), 0.78 (3H); MS (ES+) m/z 591.9.
Preparation of Example 187
Scheme 8, 8-G: wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl
Stereochemistry=(1S-cis)-L
[0262]
(1S-cis)-N-[[3-[[(2-amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethy-
lcyclopentyl]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(Example 187) (C.sub.28H.sub.33Cl.sub.2N.sub.3O.sub.6) is prepared
from 8-F (R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl) as taught by Scheme
8.
[0263] .sup.1H NMR (300 MHz, DMSO-d.sub.6) 7.74 (2H), 7.55-7.40
(4H) 7.15 (3H), 6.94 (3H), 5.16 (2H), 4.41 (1H), 3.75-3.48 (2H),
3.1-2.8 (2H), 2.63 (1H), 2.33 (1H), 1.87 (1H), 1.54 (1H), 1.32
(1H), 1.17 (3H), 1.08 (3H), 0.58 (3H); MS (FA) m/z (rel. intensity)
578 (M+H, 99), 581 (30), 580 (72), 579 (57), 578 (99), 577 (19),
504 (17) 322 (18), 239 (35), 161 (29), 159 (34); HRMS (FAB) m/z
calcd for C.sub.28H.sub.33Cl.sub.2N.sub.3O- .sub.6+H.sup.+
578.1824, found 578.1836. 72
Preparation of Example 183
Scheme 9, 9-G: wherein R.sup.4=H,
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]ph- enyl
Stereochemistry=(1S-cis)-L
[0264]
(1S-cis)-N-[[3-(carboxymethoxymethyl)-2,2,3-trimethylcyclopentyl]ca-
rbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine (Example
183) 7-A (1.15 g, 3.96 mmol) was dissolved in dry THF (5 mL). The
reaction flask was immersed in a -15.degree. C. bath (ethylene
glycol/CO.sub.2), then diborane-tetrahydrofuran (1M, 1 equiv, 3.96
mmol, 3.96 mL) was added slowly. The reaction solution was stirred
at -15.degree. C. under N.sub.2 during the day, and was
equilibrated to room temperature overnight The reaction solution
was treated with potassium carbonate (1.2 g) in H.sub.2O (25 mL).
The THF layer was separated and the aqueous phase was extracted
with EtOAc (3.times.20 mL). The organic layers were combined,
washed with saturated NaCl (20 mL) and dried (MgSO.sub.4). Solvent
was removed in vacuo from the mixture to yield 0.83 g (76%) of 9-B.
The crude product was chromatographed, initially using
CH.sub.2Cl.sub.2, as eluent followed by
CH.sub.2Cl.sub.2.backslash.MeOH (56) Recovered desired product 9-B
(0.76 g, 69%) from column: .sup.1H NMR (CDCl.sub.3) .delta. 0.80
(3H), 1.00 (3H), 1.11 (3H), 1.32-1.47 (1H), 1.62-1.91 (2H),
2.09-2.22 (1H), 2.86 (1H), 3.48-3.59 (2H), 5.08-5.18 (2H),
7.28-7.41 (5H).
[0265] A solution of benzyl ester 9-B (0.76 g, 2.75 mmol) in
CH.sub.2Cl.sub.2 (10 mL) was treated at 0.degree. C. and under N,
with ethyl diazoacetate (5 equiv, 13.75 mmol, 1.4 mL) and a
catalytic amount of HBF.sub.4 (0.1 equiv, 0.03 mL). The reaction
solution was a stirred under N.sub.2 overnight while the bath
temperature equilibrated to room temperature. Solvent was removed
under reduced pressure to yield 1.8 g of product. Crude product was
subjected to silica gel (35 mm.times.15.2 cm) flash column
chromatography, eluting with 2%-10% EtOAc/hexanes, to provide 0.64
g (64%) of 9-C: .sup.1H NMR (CDCl.sub.3) .delta. 0.81 (3H), 1.05
(3H), 1.12 (3H), 1.22-1.31 (3H), 1.32-1.43 (1H), 1.54-1.62 (2H),
1.65-1.92 (2H), 2.08-2.23 (1H), 2.85 (1H), 3.28 (1H), 3.47 (1H),
3.96-4.08 (2H), 4.14-4.25 (2H), 5.07-5.18 (2H), 7.28-7.40 (5H).
[0266] Benzyl ester 9-C (0.3 g, 0.83 mmol) was dissolved in
absolute EtOH (10 mL). The solution was treated with a catalytic
amount of 10% Pd/C (0.2 g) and hydrogenated at 20 psi for 1 h on a
Parr hydrogenation apparatus. The suspension was filtered through a
Celite cake and washed cake with EtOH. Solvent was removed from the
filtrate under reduced pressure to yield 0.19 g (84%) of 9-D:
.sup.1H NMR (CDCl.sub.3) .delta. 0.90 (3H) 1.06 (3H), 1.16 (3H),
1.19-1.32 (3H), 1.33-1.45 (1H), 1.65-1.92 (2H), 2.03-2.18 (1H) 2.83
(1H), 3.29 (1H). 3.49 (1H), 3.95-4.11 (2H), 4.19 (2H).
[0267] Dissolved acid 9-D (0.18 g, 0.66 mmol), HOBT (1.2 equiv,
0.81 mmol, 0.11 g), DMAP (0.11 equiv, 0.074 mmol, 0.009 g), EDCl
(1.2 equiv, 0.78 mmol, 0.15 g) and Et.sub.3N (3.8 equiv, 2.5 mmol,
0.35 mL) in CH.sub.2Cl.sub.2 (10 mL) at 0.degree. C. The reaction
mixture was stirred for several minutes and-then 7-E-1 (1.0 equiv,
0.69 mmol, 0.28 g) was added. The mixture was stirred overnight
while the bath temperature equilibrated to room temperature. The
reaction mixture was concentrated to dryness under reduced
pressure. The residue was quenched with acidic H.sub.2O (30 mL) and
extracted with CHCl.sub.3 (3.times.15 mL). The organic layers were
combined, washed with saturated NaHCO.sub.3 (30 mL), dried
(MgSO.sub.4) and solvent was removed under reduced pressure. Crude
product was purified by silica gel (20 mm.times.22.9 cm) flash
chromatography using 90% CH.sub.2Cl.sub.2/EtOAc as eluent.
[0268] Fractions containing the compound were combined and solvent
was removed under reduced pressure. The product was dissolved in
CH.sub.3CN:H.sub.2O, frozen and lyophilized to yield 0.18 g (44%)
of 9-F (where R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3 and
R.sup.6=C.sub.6H.sub.4OCH.- sub.2C.sub.6H.sub.3Cl.sub.2): .sup.1H
NMR (CDCl.sub.3) .delta. 0.82 (3H), 1.05 (3H), 1.12 (3H), 1.27
(2H), 1.31-1.43 (1H), 1.59-1.84 (2H), 2.05-2.21 (1H), 2.55 (1H)
3.03-3.23 (2H), 3.30 (1H), 3.47 (1H), 3.74 (3H), 3.93-4.09 (2H),
4.19 (2H), 4.90 (2H), 5.76 (1H), 7.11 (2H), 7.24-7.39 (3H), 7.47
(1H), 7.57 (2H); IR (mineral oil mull) 3292, 3193, 3123, 3064,
3033, 2951, 2922, 2870, 2855, 2854, 1748, 1657, 1656, 1606, 1579,
1562, 1537, 1515, 1461, 1431, 1414, 1376, 1348, 1324, 1271, 1207,
1196, 1153, 1129, 1023, 800, 781 cm.sup.-1; MS (FAB) for
C.sub.31H.sub.38Cl.sub.2N.sub.2O.sub.7, m/z (relative intensity)
623 ([M+H].sup.+, 49), 622 ([M+H].sup.+, 29), 621 ([M+H].sup.+,
71), 620 (M.sup.+, 71, 517 (12), 351 (16), 349 (22), 175 (16), 173
(26), 151 (27), 123 (100). Anal. Calcd for
C.sub.31H.sub.38Cl.sub.2N.sub.2O.sub.7: C, 59.91; H, 6.16; N, 4.51;
Cl, 11.41; Found: C, 59.67; H, 6.09; N, 4.63; Cl, 11.50. Corrected
for 0.40% H.sub.2O found by Karl Fischer analysis.
[0269] To a solution of methyl ester 9-F (R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=C.sub.6H.sub.4OCH.sub.2C.sub.6H.sub.3Cl- .sub.2) (0.11 g,
0.18 mmol) in MeOH (5 mL) was added LiOH.sub.2O (5 equiv, 0.88
mmol, 0.04 g) in H.sub.2O (1 mL). The reaction solution was allowed
to stir for about 2 hrs. The solvent was removed in vacuo. The
residue was dissolved in H.sub.2O (30 mL) and acidified with HCl.
The resulting precipitate was filtered and washed with H.sub.2O.
The precipitate was dissolved in 50S CH.sub.3CN:H.sub.2O (10 mL),
was frozen and lyophilized to yield 0.083 g (80%) of Example 183
(9-G: where R.sup.4=H and
R.sup.6=C.sub.6H.sub.4OCH.sub.2C.sub.6H.sub.3Cl.sub.2): .sup.1H NMR
(MeOD) .delta. 0.79 (3H), 1.00 (3H), 1.05 (3H), 1.23-1.41 (1H),
1.60-1.78 (2H), 1.91-2.05 (1H), 2.72 (1H), 2.98 (1H), 3.20 (1H),
3.46 (1H), 3.93-4.08 (2H), 4.66-4.78 (1H), 7.24 (2H), 7.38-7.51
(3H), 7.57 (2H), 7.73 (1H); IR (mineral oil mull) 3270, 3193, 3123,
3056, 3034, 2954, 2928, 2854, 1731, 1657, 1607, 1562, 1537, 1516,
1461, 1459, 1432, 1414, 1376, 1326, 1272, 1220, 1195, 1126, 800,
781 cm.sup.-1; MS (FAB) for C.sub.28H.sub.32Cl.sub.2N.sub.2O.sub.7,
m/z (relative intensity) 581 (([M+H].sup.+, 51), 580 ([M+H].sup.+,
29), 579 ([M+H].sup.+, 76), 578 (M.sup.+, 8), 337 (12), 335 (18),
175 (19), 173 (29), 161 (18), 151 (18), 123 (100). Anal. Calcd for
C.sub.28H.sub.32Cl.sub.2N.sub.2O.sub.7: C, 58.04; H, 5.57; N, 4.84;
Cl, 12.24; Found: C, 57.87; H, 5.44; N, 4.97; Cl, 12.29. Corrected
for 2.17% H.sub.2O found by Karl Fischer analysis. 73
Preparation of Example 192:
[0270] Scheme 10, 10-G: wherein R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl
Stereochemistry=(1S-cis)-L
[0271]
(1S-cis)-O-[(2,6-Dichlorophenyl)methyl]-N-[(3-hydroxy-2,2,3-trimeth-
ylcyclopentyl)carbonyl]-L-tyrosine (Example 192).
[0272] Into a 100 mL oven dried round bottom flask was placed
(1S-cis)-[3-carboxy-2,2,3-trimethyl]cyclopentane carboxylic acid
methyl ester (10-A) (5.0 g, 23 mmol, 1 equiv), followed by dry THF
(20 mL). The reaction flask was immersed in a -15.degree. C. bath
(ethylene glycol/dry ice) and then boron
trifluoride-tetrahydrofuran (1 equiv, 1 M, 23 mmol, 23 mL) was
slowly added. [Note: observed mild evolution of H.sub.2 gas]. The
reaction solution was allowed to stir for 24 hrs under N.sub.2. The
solution was stirred in the -15.degree. C. bath during the day, and
the bath was permitted to reach room temperature overnight. Silica
gel Thin-layer chromatography, using 1:1 hexanes/ethyl acetate as
eluent, showed that the starting material was consumed.
Consequently, the reaction solution was quenched with H.sub.2O (100
mL) and then treated with potassium carbonate (6 g). The THF phase
was separated while the aqueous phase was extracted with EtOAc
(3.times.80 mL). The organic phases were combined, washed with
saturated NaCl (100 mL) and dried (MgSO.sub.4). The solvent was
removed via rotary evaporator and then by overnight hi vacuum
conditions to yield 4.7 g of crude product. Crude product was
subjected to a silica gel (35 mm.times.25.4 cm) flash
chromatography column. Elution with 50 EtOAc/hexanes yielded 4.0 g
(87%) of 10-B as-a clear oil: .sup.1H NMR (CDCl.sub.3) .delta. 0.79
(3H), 0.99 (3H), 1.10 (3H), 1.32-1.43 (1H), 1.48 (H), 1.63-1.88
(2H), 2.04-2.15 (1H), 2.8 (1H), 3.52 (2H), 3.66 (3H); IR (mineral
oil mull) 3445, 2968, 2878, 1734, 1719, 1456, 1436, 1372, 1358,
1270, 1218, 1203, 1173, 1031, 1006 cm.sup.-1; MS (FAB) for
C.sub.11H.sub.20O.sub.3, m/z (relative intensity) 202
([M+2H].sup.+, 11), 201 ([M+H].sup.+, 100), 200 (M.sup.+, 0.9), 183
(11), 169 (8), 151 (8), 123 (29). Anal. for
C.sub.11H.sub.20O.sub.3: C, 65.97; H, 10.07; Found: C, 65.94; H,
9.91. Corrected for 0.85% H.sub.2O found by Karl Fischer
analysis.
[0273] (1S-cis)-[3-hydroxymethyl-2,2,3-trimethyl]-cyclopentane
carboxylic acid methyl ester 10B (1.5 g, 7.5 mmol) was dissolved in
CH.sub.2Cl.sub.2 (35 mL). Cooled flask to 0.degree. C., slowly
added chloromethyl methyl ether (3.3 equiv, 25 mmol, 1.9 mL) and
then added DIEA (5.87 mL, 4.5 equiv, 3.4 mmol). The bath
temperature was allowed to equilibrate to room temperature while
the reaction mixture was stirred for four days. Solvent was removed
via a rotary evaporator. Crude product was dissolved in toluene,
the insoluble precipitate was filtered and the concentrated
filtrate was chromatographed on silica gel (35 mm.times.5.2 cm)
flash chromatography using 2% EtOAc/hexanes as eluent to yield 1.37
g (75%) of 10-C (where R.sup.10-1=methoxymethyl): .sup.1H NMR
(CDCl.sub.3) .delta. 0.80 (3H), 1.02 3H), 1.12 (3H), 1.34-1.45
(1H), 1.57 (H), 1.67-1.87 (2H), 2.07-2.18 (1H), 2.82 (1H), 3.31
(1H), 3.36 (3H), 3.45 (1H), 3.67 (3H), 4.56-4.61 (2H).
[0274] Compound 10-C (1.57 g, 6.4 mmol) was dissolved in THF (20
mL) and treated with LiOH.H.sub.2O (10 equiv, 64 mmol, 2.7 g) in
H.sub.2O (30 mL), H.sub.2O.sub.2 (6 mL), H.sub.2O (16 mL) and MeOH
(16 mL). The mixture was refluxed overnight. The solvent was
removed in vacuo, crude residue was quenched with H.sub.2O (35 mL)
and the pH was lowered to 5 with 10%, 6N or 12N hydrochloric acid.
Extracted aqueous portion with EtOAc (3.times.20 mL) and then with
CHCl.sub.3 (3.times.20 mL). Organic layers were combined, dried
(MgSO.sub.4) and solvent removed on rotary evaporator.
[0275] Crude product was subjected to a silica gel (35
mm.times.15.2 cm) flash chromatography column using 80%
hexanes/EtOAc as eluent to yield 1.1 g (75%) of 10-D (where
R.sup.10-1=methoxy methyl): .sup.1H NMR (CDCl.sub.3) .delta. 0.89
(3H), 1.03 (3H), 1.16 (3H), 1.36-1.46, 1.68-1.89, 2.04-2.18 (1H),
2.85 (1H), 3.32 (1H), 3.36 (3H), 3.47 (1H), 4.57-4.62 (2H).
[0276] Compound 10-D (0.83 g, 3.6 mmol), HOBT (1.13 equiv, 4.1
mmol, 0.55 g), DMAP (0.11 equiv, 0.4 mmol, 0.048 g), EDCl (1.1
equiv, 4.0 mmol, 0.76 g) and Et.sub.3N (3.6 equiv, 13 mmol, 1.8 mL)
were mixed in CH.sub.2Cl.sub.2 (30 mL) at 0.degree. C. The reaction
mixture was stirred for several minutes, and then added
O-[(2,6-dichlorophenyl)methyl]-L-tyro- sine methyl ester
hydrochloride 7-E-2.HCl (wherein R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl) (1 equiv, 3.6 mmol,
1.4 g). The mixture was stirred overnight while bath temperature
equilibrated to room temperature. Reaction mixture was concentrated
to dryness under reduced pressure. The residue was quenched with
acidic H.sub.2O (70 mL), and extracted with CHCl.sub.3 (3.times.35
mL). Organic layers were combined, washed with saturated
NaHCO.sub.3 (40 mL), dried (MgSO.sub.4) and solvent was removed
under reduced pressure. Crude product was subjected to a silica gel
(35 mm.times.15.2 cm) flash chromatography column using 10%
EtOAc/hexanes as eluent to yield 1.6 g (78%) of 10-F-1 (10-F (where
R.sup.10-1=methoxymethyl, R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3 and
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]pheny- l-): .sup.1H NMR
(CDCl.sub.3, 400 MHz) .delta. 0.82 (3H), 1.03 (3H), 1.11 (3H),
1.33-1.42 (1H), 1.61 (2H), 1.63-1.82 (2H), 2.08-2.21 (1H), 2.56
(1H), 3.03-3.16 (2H), 3.31 (1H), 3.36 (3H), 3.46 (1H), 3.75 (3H),
4.57-4.61 (2H), 4.85-4.93 (1H), 5.26 (2H), 5.72 (1H), 6.96 (2H),
8.6 (2H), 7.22-7.28 (1H), 7.37 (2H); IR (mineral oil mull) 2951,
2879, 1746, 1668, 1657, 1565, 1511, 1468, 1439, 1382, 1371, 1241,
1216, 1197, 1179, 1148, 1108, 1096, 1047, 1019, 780, 768 cm.sup.-1;
MS (FAB) for C.sub.29H.sub.37Cl.sub.2N.sub.1O.sub.6, m/z (relative
intensity) 568 (M+H].sup.+, 69), 566 ([M+H]I+, 100), 565 (M.sup.+,
12), 506 (41), 504 (61), 336 (41), 161 (40), 159 (62), 123 (83), 45
(53). Anal. Calcd for C.sub.29H.sub.37Cl.sub.2N.sub.1O.sub.6: C,
61.48; H, 6.58; N. 2.47; Cl, 12.52; Found: C, 61.30; H, 6.55; N.
2.80; Cl, 12.57. Corrected for 0.11% H.sub.2O found by Karl Fischer
analysis.
[0277] Compound 10-F (0.74 g, 1.3 mmol) was dissolved in MeOH (30
mL) and treated with concentrated HCl (5 mL) and stirred at room
temperature for 24 hrs. The solvent was removed in vacuo to yield a
residue that was taken up in CHCl.sub.3 and washed with saturated
NaHCO.sub.3. [Note: upon treatment with saturated NaHCO.sub.3 a
precipitate formed which was filtered and washed with CHCl.sub.3.]
Filtrate volume was reduced in vacuo and subjected to a silica gel
(35 mm.times.16.5 cm) flash chromatography column using 50%
hexanes/EtOAc as eluent. Fractions containing pure compound were
combined and solvent was removed under reduced pressure. The
residue was dissolved in 50% CH.sub.3CN:H.sub.2O (10 mL), frozen
and lyophilized to yield 0.23 g (34%) of 10-G (where R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3 and R.sup.6=4-[(2,6-dichlorophenyl)me-
thoxy]phenyl-: .sup.1H NMR (CDCl.sub.3, 400 MHz) .delta. 0.84 (3H),
1.00 (3H), 1.10 (3H), 1.37-1.48 (1H), 1.69-1.82 (2H), 2.09-2.21
(1H), 2.55 (1H), 3.02-3.18 (2H), 3.51-3.59 (2H), 3.75 (3H),
4.84-4.91 (1H), 5.26 (2H), 5.75 (1H), 6.96 (2H), 7.05 (2H),
7.22-7.29 (1H), 7.37 (2H); IR (mineral oil mull) 3428, 3322, 2923,
2870, 2854, 1743, 1654, 1565, 1511, 1466, 1439, 1377, 1298, 1278,
1240, 1197, 1179, 1018, 1003, 780, 768 cm.sup.-1; MS (FAB) for
C.sub.27H.sub.33Cl.sub.2N.sub.1O.sub.5, m/z (relative intensity)
524 ([M+H].sup.+, 65), 523 ([M+H].sup.+, 41), 522 ([M+H]-F, 100),
521 (M.sup.+, 19), 354 (21), 338 (21), 336 (29), 161 (21), 159
(34), 123 (38). Anal. Calcd for C.sub.27H.sub.33Cl.sub.2N.sub.1-
O.sub.5: C, 62.07; H, 6.37; N, 2.68; Cl, 13.57; Found: C, 61.85; H,
6.27; N, 2.85; Cl, 13.50. Corrected for 0.44% H.sub.2O found by
Karl Fischer analysis.
[0278] Compound 10-G (0.19 g, 0.36 mmol) was dissolved in
CH.sub.3OH (4 mL) and treated with LiOH.H.sub.2O (10 equiv, 0.15 g,
3.6 mmol) in H.sub.2O (4 mL). Additional MeOH (2 mL) was added to
ensure solubility. The solution was stirred for 2 h at room
temperature. The solvent was removed under reduced pressure. The
residue was dissolved in warm H.sub.2O (20 mL) (Note: room
temperature H.sub.2O caused aqueous solution to gel). The pH of the
solution was lowered to 2 with 1.0 N HCl and the resulting
precipitate was filtered, washed with H.sub.2O and dried to yield
0.17 g (93.) of Example 192 (10-G: where R.sup.4=H.
R.sup.5=CO.sub.2H and
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl-): unable to
determine mp due to compound shrinkage at 80 C; .sup.1H NMR
(CDCl.sub.3) .delta. 0.81 (3H), 0.97 (3H), 1.06 (3H), 1.35-1.48
(1H), 1.65-1.88 (2H), 2.03-2.18 (1H), 2.55 (1H), 3.04-3.27 (2H),
3.48-3.58 (2H), 4.81-4.89 (1H), 5.24 (2H), 5.85 (1H), 6.96 (2H),
7.12 (2H), 7.19-7.29 (1H), 7.32-7.38 (2H); IR (mineral oil mull)
3421, 3332, 3058, 3030, 2954, 2920, 2871, 2855, 1729, 1653, 1612,
1585, 1565, 1511, 1466, 1439, 1377, 1299, 1241, 1196, 1179, 1018,
1003, 779, 769 cm.sup.4; MS (FAB) for
C.sub.26H.sub.31Cl.sub.2N.sub.1O.sub.5 m/z (relative intensity) 510
([M+H].sup.+, 66), 509 ([M+H].sup.+, 37), 508 ([M+H.sup.+, 100),
507 (M.sup.+, 15), 340 (12), 324 (15), 322 (22), 161 (23), 159
(36), 123 (45). Anal. Calcd for
C.sub.26H.sub.31Cl.sub.2N.sub.1O.sub.5: C, 61.42; H, 6.15; N, 2.76;
Cl, 13.95; Found: C, 61.33; H, 6.16; N, 2.93; Cl, 13.74. Corrected
for 1.61% H.sub.2O found by Karl Fischer analysis.
Preparation of Example 198:
Scheme 10, 10-F=wherein R.sup.10-1=methyl, R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl
Stereochemistry=(1S-cis)-L
[0279]
(1S-cis)-O-[(2,6-Dichlorophenyl)methyl]-N-[(3-methoxymethyl-2,2,3-t-
rimethylcyclopentyl)carbonyl]-L-tyrosine (Example 198).
[0280] Alcohol 10-B (1.0 g, 4.8 mmol), in Et.sub.2O (20 mL), was
treated with boron trifluoride dimethyl etherate (0.1 equiv, 0.48
mmol, 0.06 mL) and an excess amount of CH.sub.2N.sub.2Et.sub.2O.
The reaction mixture was stirred overnight. The mixture was
filtered and solvent was removed on rotary evaporator. Crude
product was subjected to a silica gel (35 mm.times.16.5 cm) flash
chromatography column using 1% EtOAc/pentane as eluent to yield
0.69 g (67%) of 10C (where R.sup.10-1=methyl): .sup.1H NMR
(CDCl.sub.3) .delta. 0.79 (3H), 1.00 (3H), 1.10 (3H), 1.32-1.43
(1H), 1.60-1.89 (2H) 2.04-2.20 (1H), 2.80 (1H), 3.16 (1H), 3.28
(1H), 3.31 (3H), 3.68 (3H).
[0281] Ester 10-C (0.37 g, 1.7 mmol) was dissolved in THF (40 mL)
and treated with LiOH.H.sub.2O (10 equiv, 17 mmol, 0.71 g) in
H.sub.2O (20 mL), MeOH (10 mL) and H.sub.2O.sub.2 (10 mL). The
mixture was heated at reflux for about 8 h. Solvent was removed via
a rotary evaporator. Dissolved residue in H.sub.2O (50 mL), lowered
pH to 5 with hydrochloric acid, extracted aqueous portion with
EtOAc (3.times.25 mL) followed by CHCl.sub.3 (3.times.25 mL).
Organic extracts were combined, dried (MgSO.sub.4), and solvent was
removed under reduced pressure to yield 0.44 g of 10-D (wherein
R.sup.10-1=Me): .sup.1H NMR (CDCl.sub.3) .delta. 0.86 (3H), 1.00
(3H), 1,14 (3H), 1.34-1.44 (1H), 1.62-1.89 (2H), 2.01-2.15 (1H),
2.83 (1H), 3.16 (1H), 3.29 (1H), 3.30 (3H).
[0282] Compound 10-D (0.25 g, 1.2 mmol), HOBT (1.13 equiv, 1.41
mmol, 0.19 g), DMAP (0.11 equiv, 0.14 mmol, 0.017 g), EDC (1.1
equiv, 1.37 mmol, 0.25 g) and Et.sub.3N (3.6 equiv, 4.49 mmol, 0.6
mL) were mixed in CH.sub.2Cl.sub.2 (10 mL) at 0.degree. C. Stirred
the reaction mixture for several minutes then added 7-E-2.HCl (0.8
equiv, 1.0 mmol, 0.4 g). The mixture was stirred overnight while
the bath temperature equilibrated to room temperature. Concentrated
reaction mixture to dryness under reduced pressure. Residue was
treated with acidic H.sub.2O (30 mL) and extracted with CHCl.sub.3
(3.times.15 mL) Combined organic layers, washed with saturated
NaHCO.sub.3 (20 mL), dried (MgSO.sub.4) and solvent was removed
under reduced pressure. Crude product was purified by silica gel
(20 mm.times.16.5 cm) flash chromatography using 10%-15%
EtOAc/hexanes as eluent. Fractions containing the compound were
combined and solvent was removed under reduced pressure. The
residue was dissolved in 50% 6 CH.sub.2CN:H.sub.2O (50 mL), frozen
and lyophilized to yield 0.24 g (45%) of 10-F (where
R.sup.10-1=methyl, R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3 and
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl-): .sup.1H NMR
(CDCl.sub.3) .delta. 0.79 (3H), 0.99 (3H), 1.09 (3H), 1.32-1.43
(1H), 1.61 (2H), 1.65-1.85 (2H), 2.08-2.23 (1H), 2.53 (1H),
3.01-3.18 (2H), 3.16 (1H), 3.28 (1H), 3.30 (3H), 3.74 (3H),
4.83-4.91 (1H), 5.25 (2H), 5.74 (1H), 6.96 (2H). 7.05 (2H),
7.23-7.29 (1H), 7.35-7.40 (2H): IR (mineral oil mull) 3317, 2956,
2924, 2871, 2857, 2855, 1745, 1652, 1612, 1565, 1511, 1466, 1439,
1378, 1298, 1278, 1240, 1197, 1178, 1106, 1096, 1017, 1000, 779,
768 cm.sup.-1; MS (FAB) for C.sub.28H.sub.35Cl.sub.2N.sub.1O.sub.5,
m/z (relative intensity) 538 (([M+H], 65), 537 ([M+H].sup.+, 40),
536 ([M+H].sup.+, 100), 535 (M.sup.+, 16), 338 (16), 336 (24), 161
(18), 159 (28), 123 (69). Anal. Calcd for
C.sub.28H.sub.35Cl.sub.2N.sub.2O.sub.5 nH.sub.2O: C, 62.69; H,
6.58; N, 2.61; Cl, 13.22; Found: C, 62.44; H, 6.35; N, 2.88; Cl,
13.11. Corrected for 0.37% H.sub.2O found by Karl Fischer
analysis.
[0283] Ester 10-F (0.27 g, 0.5 mmol) was treated with LiOH.H.sub.2O
(10 equiv, 5.0 mmol, 0.21 g) in H.sub.2O (8 mL). The reaction
mixture was stirred for 1 h. THF (3 mL) was added to complete
dissolution of starting material and MeOH (3 mL) was added to
convert reaction mixture to homogeneous solution. The volume was
reduced under vacuum. H.sub.2O (40 mL) was added and the mixture
was acidified with HCl. The resulting precipitate was filtered,
washed with H.sub.2O, and dried under house vacuum to yield 0.26 g
(99%) of Example 198 (10-F: where R.sup.10-1=methyl; R.sup.4=H;
R.sup.5=CO.sub.2H and R.sup.6=4[(2,6 dichlorophenyl)
methoxy]phenyl-stereochemistry=(1S-cis)-L): .sup.1H NMR
(CDCl.sub.3) .delta. 0.77 (3H), 0.98 (3H), 1.05 (3H), 1.31-1.42
(1H), 1.61-1.85 (2H), 2.01-2.16 (1H), 2.53 (1H), 3.07-3.28 (2H),
3.15 (1H), 3.27 (L H), 3.29 (3H), 4.79-4.87 (1H), 5.24 (2H), 5.77
(1H), 6.97 (2H), 7.12 (2H), 7.21-7.29 (1H), 7.32-7.39 (2H); IR
(mineral oil mull) 3424, 3335, 3032, 2955, 2924, 2855, 1734, 1644,
1612, 1586, 1565, 1512, 1466, 1439, 1377, 1299, 1241, 1197, 1179,
1107, 1096, 1018, 873, 779, 769 cm.sup.-1; MS (FAB) for
C.sub.27H.sub.33Cl.sub.2N.sub.1O.sub.5, m/z (relative intensity)
524 (([M+H].sup.+, 62), 523 ([M+H].sup.+, 36), 522 ([M+H].sup.+,
96), 521 (M.sup.+, 13), 324 (14), 322 (22), 161 (31), 159 (42), 123
(100). Anal. Calcd for C.sub.27H.sub.33C.sub.12N.sub.1O.sub.5nH-
.sub.2O: C, 62.07; H, 6.37; N, 2.68; Cl, 13.57; Found: C, 62.04; H,
6.24; N, 2.90; Cl, 13.91. Corrected for 2.18% H.sub.2O found by
Karl Fischer analysis.
Preparation of Example 203:
Scheme 10, 10-F; where R.sup.10-1=methoxymethyl; R.sup.4=H;
R.sup.5=CO.sub.2H and
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl-,
Stereochemistry=(1S-cis)-L
[0284] (1S-cis)-O
[(2,6-Dichlorophenyl)methyl]-N-[(3-methoxymethoxymethyl--
2,2,3-trimethylcyclopentyl) carbonyl]-L-tyrosine (Example 203)
Compound 10-F-1 (10-F: (where R.sup.10-1=methoxymethyl, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3 and R.sup.6=4
(2,6-dichlorophenyl)methoxy]phenyl- -) (0.25 g, 0.44 mmol) was
dissolved in MeOH (3 mL). Treated the reaction solution with
LiOH.H.sub.2O (10 equiv, 4.4 mmol, 0.18 g) in H.sub.2O (5 mL).
Additional MeOH (2 mL) was added to ensure solubility. [Note:
reaction mixture turned clear within 1 hour of reaction time].
Solution was allowed to stir overnight at room temperature under
N.sub.2. Solvent volume was reduced on a rotary evaporator, the
remaining material was diluted with H.sub.2O (10 mL) and acidified
with hydrochloric acid. The resulting precipitate was filtered,
washed with H.sub.2O and dried under house vacuum to yield 0.23 g
(95%) of Example 203. mp: unable to determine due to compound
shrinkage at 60.degree. C.; .sup.1H NMR (CDCl.sub.3) .delta. 0.79
(3H), 1.01 (3H), 1.07 (3H), 1.32-1.46 (1H), 1.62-1.84 (2H),
2.01-2.18 (1H), 2.55 (1H), 3.02-3.24 (2H), 3.30 (1H), 3.35 (3H),
3.45 (1H), 4.55-4.61 (2H), 4.81-4.89 (1H), 5.24 (2H), 5.76 (1H),
6.97 (2H), 7.12 (2H), 7.18-7.27 (1H), 7.31-7.37 (2H); IR (mineral
oil mull) 2930, 2872, 2855, 1736, 1638, 1612, 1511, 1466, 1439,
1377, 1241, 1196, 1179, 1147, 1108, 1045, 1020 cm.sup.-1; MS (FAB)
for C.sub.28H.sub.35Cl.sub.2N.sub.1O.sub.6nH.sub.2O, m/z (relative
intensity) 554 (([M+H].sup.+, 67), 552 ([M+H].sup.+, 100), 551
(M.sup.+, 16), 492 (38), 490 (56), 322 (33), 161 (40), 159 (64),
123 (73), 45 (61). Anal. Calcd for
C.sub.28H.sub.35Cl.sub.2N.sub.1O.sub.6: C, 60.87; H, 6.39; N, 2.54;
Cl, 12.83; Found: C, 60.73; H, 6.41; N, 2.69; Cl, 12.86. Corrected
for 0.11% H.sub.2O found by Karl Fischer analysis. 74
Preparation 11-B
Scheme 11, 11-B Stereochemistry=1S-cis
[0285] (1S-cis) 3-Formyl-2,2,3-trimethylcyclopentanecarboxylic acid
methyl ester (11-B) (C.sub.11H.sub.20O.sub.3)
[0286] To a solution of 10-B (2.57 g, 12.8 mmol) in methylene
chloride (50 mL) is added a mixture of pyridinium chlorochromate
(3.05 g, 14.15 mmol), magnesium sulfate (4 g, 33 mmol), and Celite.
After overnight stirring, the mixture is eluted through a short
column of silica gel (80 g) using methylene chloride (500 mL) as
the eluant. Evaporation in vacuo gives 11-B as a colorless liquid
(2.09 g, 82% yield).
[0287] .sup.1H NMR (CDCl.sub.3) .delta. 9.65 (1H), 3.67 (3H), 2.81
(1H), 2.40 (1H), 2.23 (1H), 1.94 (1H), 1.42 (1H), 1.16 (3H), 1.05
(3H), 0.88 (3H).
Preparation 11-C
Scheme 11, 11-C Stereochemistry=1S-cis, (E)
[0288]
(1S-cis)-3-[2-[(1,1-dimethylethyoxy)carbonyl]ethenyl]-2,2,3-trimeth-
ylcyclopentanecarboxylic acid methyl ester (11-C)
(C.sub.17H.sub.28O.sub.4- )
[0289] To a dry N.sub.2 flushed 100 mL flask is added t-butyl
diethyl phosphonoacetate (5 mL, 21.3 mmol) and THF (dry, 20 mL).
The flask is immersed in an ice water bath and, five minutes later,
NaH/oil (60% NaH, 0.5 g, 12.5 mmol) is added in portions. After
thirty minutes, 11-B (2.08 g, 10.5 mmol) is mixed with THF (dry, 15
mL) and added via syringe over a five minute period. Four hours
later, the still cold solution is diluted with toluene (200 mL),
shaken with ice water (4.times.100 mL), and the organic layer
evaporated to dryness in vacuo, giving a colorless oil which is
chromatographed on silica gel (80 g) using a gradient from 0 to 46
ethylacetate/hexane. A colorless oil 11-C is obtained (1.99 g, 63%
yield).
[0290] .sup.1H NMR (CDCl.sub.3) .delta. 6.92 (1H), 5.68 (1H), 3.67
(3H), 2.84 (1H), 2.23 (1H), 1.99 (1H), 1.90 (1H), 1.49 (1H), 1.48
(9H), 1.06 (3H), 1.01 (3H), 0.71 (3H); IR (nujol) 1728, 1712, 1651,
1438, 1358, 1316, 1288, 1270, 1228, 1220, 1191, 1171, 1151, 1132,
1001 cm.sup.-1; MS (FAB) m/z (rel. intensity) 297 (M+H, 44), 297
(44), 242 (12), 241 (87), 224 (14), 223 (99), 195 (14), 191 (14),
135 (12), 57 (34), 41 (12); HRMS (FAB) calcd for
C.sub.17H.sub.28O.sub.4 +H1 297.2065, found 297.2067; Anal. Calcd
for C.sub.17H.sub.28O.sub.4: C, 68.89; H, 9.52; N; Found: C, 69.03;
H, 9.18.
Preparation 11-D
Scheme 11, 11-D Stereochemistry=1S-cis, (E)
[0291]
(1S-cis)-3-[2-[(1,1-dimethylethoxy)carbonyl]ethenyl]-2,2,3-trimethy-
lcyclopentanecarboxylic acid (11-D) (C.sub.16H.sub.26O.sub.4)
[0292] A solution of LiOH.H.sub.2O(0.65 g, 15.4 mmol) in H.sub.2O
(15 mL) and aqueous H.sub.2O.sub.2 (5 mL, 30%) is added to a
solution of 11-C (1.52 g, 5.13 mmol) in methanol (30 mL). After
stirring for two days, the mixture is diluted with water (100 mL)
and evaporated in vacuo until all of the methanol is gone. The
aqueous remainder is then shaken with diethyl ether (3.times.40 mL)
and then cooled in an ice water bath and brought to pH 5 using 1N
aq. HCl. The resultant white precipitate 11-D is isolated by
suction filtration (0.385 g, 26% yield)
[0293] .sup.1H NMR (CDCl.sub.3): .delta. 6.94 (1H), 5.69 (1H), 2.88
(1H), 2.27-1.84 (3H), 1.53 (1H), 1.49 (9H), 1.11 (3H), 1.02 (3H),
0.81 (3H); IR (nujol) 2729, 2669, 1707, 1647, 1418, 1393, 1316,
1305, 1242, 1154, 999, 976, 960, 944, 856 cm.sup.-1; MS (FAB) m/z
(rel. intensity) 283 (M+H, 34), 566 (13), 283 (34), 228 (13), 227
(99), 210 (13), 209 (96), 191 (14), 181 (14), 57 (51), 41 (16);
Anal. Calcd for C.sub.16H.sub.26O.sub.4- : C, 68.06; H, 9.28;
Found: C, 67.84; H, 9.10.
Preparation 11-F-1
Scheme 11, 11-F: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl
Stereochemistry=[1S-[1.alpha- .,3.alpha.(E)]]-L
[0294]
[S-[1.alpha.,3.alpha.(E)]]-N-[[3-[2-[(1,1-dimethylethoxy)carbonyl]e-
thenyl]-2,2,3-trimethylcyclopentyl]carbonyl]l-4-[(2,6-dichlorobenzoyl)amin-
o]-L-phenylalanine methyl ester (11-F-1)
(C.sub.33H.sub.38Cl.sub.2N.sub.2O- .sub.6)
[0295] A solution of 11-D (0.45 g, 1.59 mmol) in methylene chloride
(15 mL) is cooled in an ice water bath. To this stirred solution
were added N,N-diisopropylethyl amine (2 mL, 11.48 mmol), EDC
(0.335 g, 1.75 mmol), HOBt (0.25 g, 1.85 mmol), and
4-N,N-dimethylaminopyridine (0.02 g, 0.16 mmol), followed thirty
minutes later by the 4-[(2,6-dichlorobenzoyl)amino-
]-L-phenylalanine methyl ester hydrochloride 7-E-1.HCl (7-E:
R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl) (0.67 g, 1.66 mmol).
After two days, the reaction mixture is evaporated to dryness,
mixed with toluene (200 mL), and shaken with cold 0.5N aq. HCl (50
mL) followed by water (4.times.50 mL). The organic layer is then
evaporated to dryness in vacuo to give an off-white solid which is
recrystallized from ethyl acetate to give a white solid (11-F-1)
(0.59 g, 58% yield).
[0296] .sup.1H NMR (CDCl.sub.3) .delta. 7.84 (1H), 7.56 (2H),
7.35-7.25 (3H), 7.08 (2H), 6.90 (1H), 5.77 (1H), 5.65 (1H), 4.87
(1H), 3.73 (3H), 3.12 (2H), 2.59 (1H), 2.21 (1H), 2.05 (1H), 1.85
(1H), 1.49 (1H), 1.47 (9H), 1.03 (3H), 0.99 (3H), 0.69 (3H); MS
(ES+) m/z 631.
Preparation 11-G-1
Scheme 11, 11-G: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl
Stereochemistry=[1S-[1.alph- a.,3.alpha.(E)]]
[0297]
[1S-[1.alpha.,3.alpha.(E)]]-N-[[3-(2-Carboxyethenyl)-2,2,3-trimethy-
lcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (11-G-1) (C.sub.29H.sub.30Cl.sub.2N.sub.2O.sub.6)
[0298] A solution of 11-F-1 (0.5 g, 0.8 mmol) in trifluoroacetic
acid (3 mL) is stirred overnight under nitrogen. The solution is
then evaporated to dryness in vacuo, diluted with toluene (50 mL)
and evaporated to dryness again, giving 11-G-1 as an oil (0.45 g,
97% yield).
[0299] .sup.1H NMR (300 MHz, DMSO-d.sub.6) 10.75 (1H), 10.60 (1H),
7.96 (1H), 7.64-7.44 (4H), 7.23-7.14 (3H), 6.88 (1H), 5.63 (1H),
4.9 (1H), 4.8 (1H), 4.50 (1H), 3.58 (3H), 3.03-2.70 (2H), 2.50
(1H), 2.1-1.2 (4H), 0.92 (3H), 0.83 (3H), 0.55 (3H).
Preparation of Example 182
Scheme 11, 11-G: wherein R.sup.4=H, R.sup.5=CO.sub.2H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl
Stereochemistry=[1S-[1.alph- a.,3.alpha.( (E)]]
[0300]
[1S-[1.alpha.,3.alpha.(E)]]-N-[[3-(2-Carboxyethenyl)-2,2,3-trimethy-
lcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
disodium salt (Example 182)
(C.sub.28H.sub.28Cl.sub.2Na.sub.2N.sub.2O.sub- .6)
[0301] To a solution 11-G-1 (0.45 g, 0.78 mmol) in methanol (5 mL),
in a flask cooled in an ice water bath, is added a solution of
LiOH.H.sub.2O (0.127 g, 3 mmol) in H.sub.2O (5 mL) After two days,
the mixture is diluted with water (50 mL), evaporated in vacuo
until the methanol is gone and then cooled to -10.degree. C. and
brought to pH 2 using 1N aq. HCl. The resultant white precipitate
is isolated by suction filtration to give a white solid which is
stirred with saturated aqueous NaHCO.sub.3 (2 mL) and then
transferred to a C-18 reversed phase HPLC column and eluted with a
gradient from 0.01% aq NaHCO.sub.3 to 10% acetonitrile/0.01% aq
NaHCO.sub.3. Evaporation is accomplished in vacuo to give Example
182 as a white solid (0.25 g, 51% yield).
[0302] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 7.51-7.42 (5H),
7.06 (2H), 6.39 (1H), 5.49 (1H), 4.10 (1H), 2.99 (1H), 2.86 (1H),
2.56 (1H), 1.85 (2H), 1.61 (1H), 1.29 (1H), 0.90 (3H), 0.85 (3H),
0.51 (3H); IR (nujol) 3393, 3257, 3124, 3035, 1654, 1604, 1562,
1544, 1515, 1431, 1398, 1325, 799, 778, 722 cm.sup.-1; MS (FAB) m/z
(rel. intensity) 605 (M+H, 44), 629 (9), 627 (14), 608 (8), 607
(30), 606 (14), 605 (44), 585 (14), 583 (21), 73 (45), 23 (99); KF
Water: 7.09%. 75
Preparation of Example 184
Scheme 12, 12-E: wherein R.sup.4=H, R.sup.5=CO.sub.211,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl
Stereochemistry=(1S-cis)-L
[0303]
(1S-cis)-N-[[3-Cyano-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-d-
ichlorobenzoyl)amino]l-L-phenylalanine Ester 10-A (5.0 g, 23.3
mmol) was dissolved in dry pyridine (100 mL). At 0.degree. C., the
reaction solution was treated with methanesulfonyl chloride (MsCl)
(1 equiv, 23.3 mmol, 1.8 mL). The mixture was stirred for two days,
while the bath temperature equilibrated to room temperature. The
reaction mixture was saturated with ammonia (NH.sub.3) gas. Excess
NH.sub.3 was removed via a rotary evaporator. The reaction mixture
was treated with MsCl (10 equiv. 18 mL, 0.23 mol) and allowed to
stir overnight. Solvent was removed under reduced pressure and
resulting crude material was purified by a silica gel (35
mm.times.15.2 cm) flash column chromatography using 90%
hexanes:EtOAc as eluent to yield 3.5 g of 12-B: .sup.1H NMR
(CDCl.sub.3) .delta. 1.08 (3H), 1.19 (3H), 1.32 (3H), 1.73-2.01
(2H), 2.25-2.41 (2H), 2.71 (1H), 3.70 (3H).
[0304] Ester 12-B (0.7 g, 3.6 mmol) was dissolved in THF (20 mL)
and treated with LiOH.H.sub.2O (10 equiv, 1.5 g, 35.8 mmol) in
H.sub.2O (20 mL) and MeOH (10 mL) After 2 h, the solvent was
removed in vacuo. The residue was dissolved in acidic H.sub.2O (50
mL), for example 1N HCl, 10% H.sub.2SO.sub.4, or 1N AcOH, and was
extracted with CHCl.sub.3 (3.times.20 mL) to yield, upon usual
work-up, 0.6 g (92%) of 12-C: .sup.1H NMR (CDCl.sub.3) .delta. 1.17
(3H), 1.25 (3H), 1.34 (3H), 1.77-2.00 (2H), 2.19-2.41 (2H),
2.69-2.81 (1H).
[0305] Acid 12-C (0.6 g, 3.3 mmol), HOBT (1.13 equiv, 0.5 g), DMAP
(0.11 equiv, 0.04 g), EDC (1.1 equiv, 0.7 g,), Et.sub.3N (3.6
equiv, 1.6 mL) and CH.sub.2Cl.sub.2 (20 mL) were combined and the
reaction was stirred for a couple of minutes. Then 7-E-1.HCl (0.75
equiv, 1.0 g) was added and the reaction mixture was stirred
overnight at room temperature. The solvent was removed under
reduced pressure and the residue was triturated with THF. The
precipitate was filtered, dissolved in a mixture of
CH.sub.3CN:H.sub.2O, frozen and lyophilized to yield 12-E (where
R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3 and
R.sup.6=4-[(2,6-dichlorobenzoyl)a- mino]phenyl): mp 170-174.degree.
C.; .sup.1H NMR (MeOD) .delta. 1.01 (3H), 1.17 (3H), 1.32 (3H),
1.72-1.87 (2H), 2.02-2.32 (2H), 2.67 (1H), 2.97 (1H), 3.18 (1H),
3.71 (3H), 4.73 (1H), 7.22 (2H), 7.38-7.50 (3H), 7.59 (2H); IR
(mineral oil mull) 3282, 3252, 3314, 3192, 3125, 3075, 2954, 2925,
2870, 2855, 2235, 1750, 1738, 1653, 1610, 1562, 1541, 1516, 1458,
1431, 1415, 1379, 1332, 1274, 1260, 1243, 1231, 1214, 1195, 799
cm.sup.-1; MS (FAB) for C.sub.27H.sub.29Cl.sub.2N.sub.3O.sub.4, m/z
(relative intensity) 532 ([M+H]I+, 68), 531 ([M+H].sup.+, 35), 530
([M+H].sup.+, 100), 529 (M.sup.+, 7), 351 (26), 349 (40), 175 (21),
173 (33), 136 (35), 109 (20). Anal. Calcd for
C.sub.27H.sub.29Cl.sub.2N.sub.3- O.sub.4nH.sub.2O: C, 61.14; H,
5.51; N, 7.92; Cl, 13.37; Found: C, 61.32: H, 5.53; N, 7.94; Cl,
13.04. Corrected for 0.66% H.sub.2O found by Karl Fischer
analysis.
[0306] To a solution of the obtained 12E (1.0 g, 1.9 mmol), in MeOH
(25 mL) was added LiOH.H.sub.2O (5 equiv, 9.4 mmol, 0.4 g) in
H.sub.2O (10 mL). The reaction solution was allowed to stir for 4
h. The reaction solution was reduced in vacuo to dryness. The
residue was treated with n acidic H.sub.2O (25 mL). The resulting
precipitate was filtered and subjected to silica gel (35
mm.times.15.2 cm) flash chromatography column using CH.sub.3CN
spiked with 0.1% acetic acid as an eluent. Fractions containing
compound were combined and solvent was removed under reduced
pressure. The residue was then dissolved in CH.sub.3CN:H.sub.2O,
frozen and lyophilized to yield 0.62 g (63%) of Example 184 (12-E:
where R.sup.4=H, R.sup.5=CO.sub.2H and
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]ph- enyl): mp: unable to
determine due to compound shrinkage at 50.degree. C.; .sup.1H NMR
(MeOD) .delta. 1.02 (3H), 1.18 (3H), 1.31 (3H), 1.72-1.87 (2H),
2.01-2.33 (2H), 2.69 (1H), 2.97 (1H), 3.23 (1H), 4.68-4.78 (1H),
7.24 (2H), 7.38-7.49 (3H), 7.58 (2H), 7.96 (1H); IR (mineral oil
mull) 3338, 3291, 3260, 3200, 3132, 3079, 3039, 2954, 2914, 2854,
2253, 1746, 1672, 1657, 1611, 1579, 1560, 1544, 1516, 1466, 1457,
1431, 1416, 1397, 1379, 1328, 1282, 1271, 1222, 1210, 1196, 1125,
812, 782, 800 cm.sup.-1; MS (FAB) for
C.sub.26H.sub.27CI.sub.2N.sub.3O.sub.4, m/z (relative intensity)
518 ([M+HI 68), 517 ([M+H].sup.+, 35), 516 ([M+H].sup.+, 100), 515
(M.sup.+, 7), 337 (16), 335 (24), 175 (18), 173 (27), 136 (23), 109
(12). Anal. Calcd for
C.sub.26H.sub.27Cl.sub.2N.sub.3O.sub.4nH.sub.2O: C, 60.47; H, 5.27;
N, 8.14; Cl, 13.73; Found: C, 60.33; H, 5.25; N, 8.03; Cl, 13.62.
Corrected for 4.58% H.sub.2O found by Karl Fischer analysis 76
[0307] The Intermediate amine according to Example 56 (619 mg, 1.43
mmol) was dissolved in CH.sub.2Cl.sub.2 (5 mL) containing pyridine
(0.3 mL, 3.58 mmol). To this solution 2,4,6-trichlorobenzoyl
chloride (246 mg, 1.58 mmol) was added and the solution stirred at
RT for 6 h. The reaction was acidified with 1 N HCl (20 mL) and
extracted with CH.sub.2Cl.sub.2 (3.times.20 mL). The combined
organics were dried (Na.sub.2SO.sub.4), filtered, and the solvent
removed in vacuo. The residue was chromatographed (SiO.sub.2,
gradient elution: 100% hexanes.fwdarw.50% EtOAc/hexanes) to provide
Example 58 (866 mg, 95%) as a colorless foam: .sup.1H NMR (300 MHz,
CDCl.sub.3), .delta. 7.56 (2H), 7.33 (2H), 7.08 (2H), 5.84 (1H),
4.8-4.9 (1H), 3.74 (3H), 3.0-3.2 (3H), 2.4-2.6 (2H), 2.0-2.2 (1H),
1.6-1.8 (1H), 1.43 (9H), 1.21 (3H), 1.14 (3H), 0.76 (3H) .sup.13C
NMR (75 MHz, CDCl.sub.3), .delta. 174.92, 172.55, 172.00, 161.81,
136.29, 135.91, 134.46, 133.00, 132.69, 129.75, 128.08, 120.51,
80.19, 56.60, 54.33, 53.04, 52.35, 46.32, 37.14, 32.26, 27.99,
22.90, 22.39, 21.90, 20.50; ESMS (m/z) 639 (MH.sup.+).
[0308] Example 58 (810 mg, 1.27 mmol) was dissolved in
CH.sub.2Cl.sub.2 (5 mL) containing TFA (5 mL). This reaction
mixture was stirred for 6 h at RT. Evaporation of the solvent under
reduced pressure provided a crude oil. The residue was
chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.50% EtOAc/hexanes) to provide Example 92 (703 mg, 95
O) as a colorless foam: .sup.1H NMR (300 MHz, Acetone-d.sub.6),
.delta. 9.68 (1H), 7.66 (2H), 7.44 (2H), 7.17 (2H), 6.93 (1H), 4.88
(1H), 3.72 (3H), 3.17 (1H), 3.05 (1H), 2.79 (1H), 2.5-2.6 (1H),
2.1-2.2 (1H), 1.7-1.8 (1H), 1.4-1.5 (1H), 1.25 (3H), 1.21 (3H),
0.84 (3H); .sup.13C NMR (75 MHz, Acetone-d.sub.6), .delta. 176.92,
172.14, 171.14, 160.87, 136.16, 134.58, 134.48, 132.16, 132.12,
128.93, 127.08, 119.23, 59.24, 55.25, 52.60, 51.06, 45.64, 36.03,
31.71, 21.92, 21.63, 20.83, 20.06; ESMS (m/z) 583 (MH.sup.+).
[0309] Example 92 (684 mg, 1.17 mmol) was dissolved in H.sub.2O (8
mL) containing LiOH (127 mg, 5.27 mmol). After 6 h at RT the
mixture was acidified with 3 N HCl (3 mL) and the precipitate
filtered and washed with cold H.sub.2O (3 mL). Drying under high
vacuum provided Example 93 (547 mg, 82%) as a colorless solid:
.sup.1H NMR (300 MHz, Acetone-d.sub.6), .delta. 9.96 (1H), 7.70
(2H), 7.58 (2H), 7.30 (2H), 4.8-4.9 (1H), 3.23 (1H), 3.07 (1H),
2.89 (1H), 2.5-2.6 (1H), 2.0-2.2 (1H), 1.6-1.7 (1H), 1.4-1.5 (1H),
1.26 (3H), 1.19 (3H), 0.81 (3H); .sup.13C NMR (75 MHz,
Acetone-d.sub.6), .delta. 178.52, 174.10, 173.62, 162.22, 138.12,
136.08, 134.47, 133.69, 130.77, 128.90, 120.54, 56.92, 54.56,
53.89, 47.24, 37.48, 33.37, 23.52, 23.04, 22.31, 21.73; ESMS (m/z)
569 ([M-H].sup.-). 77
[0310] The compound according to Example 56 (810 mg, 1.87 mmol) was
dissolved in Pyr/CH.sub.2Cl.sub.2 (5 mL of each) and O-nitrobenzoyl
chloride (383 mg, 2.06 mmol) was added under dry N.sub.2. The
reaction was stirred for 4 hours at RT. The solvent was evaporated
under high vacuum and 1N HCl (20 mL) was added to the residue. This
was extracted with CH.sub.2Cl.sub.2 (3.times.25 mL) and the
combined organic phases were dried over Na.sub.2SO.sub.4. The
solution was filtered, solvent evaporated and the residue
chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.50% EtOAc/hexanes) to provide the intermediate
(14-A) (808 mg, 74%): .sup.1H NMR (300 MHz, CDCl.sub.3), .delta.
8.4-8.6 (1H), 8.03 (1H), 7.6-7.7 (3H), 7.49 (2H), 7.04 (2H), 5.86
(1H), 4.7-4.8 (1H), 3.71 (3H), 3.0-3.2 (2H), 2.4-2.6 (2H), 2.0-2.1
(1H), 1.6-1.7 (1H), 1.4-1.5 (1H), 1.42 (9H), 1.20 (3H), 1.12 (3H),
0.73 (3H); .sup.13C NMR (75 MHz, CDCl.sub.3), & 174.94, 172.61,
171.97, 164.57, 146.15, 136.64, 133.77, 132.76, 132.38, 130,56,
129.62, 128.63, 124.47, 120.56, 80.16, 56.58, 54.23, 53.05, 52.28,
46.31, 37.07, 32.23, 27.96, -22.83, 22.31, 21.85, 20.50; FABMS
(m/z) 582 (MH.sup.+).
[0311] The above compound, (14-A) (706 mg, 1.21 mmol), was
dissolved in CH.sub.2Cl.sub.2 (5 mL) and TFA (15 mL) was added with
stirring at RT. After 4 hours the solvent was evaporated and the
residue chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.100% EtOAc) to provide the mono methyl ester Example
101 (623 mg, 98%): .sup.1H NMR (300 MHz, Acetone-d.sub.6), .delta.
9.79 (1H), 8.11 (1H), 7.7-7.9 (6H), 7.22 (2H), 4.81 (1H), 3.68
(3H), 3.15 (1H), 3.02 (1H), 2.86 (1H), 2.53 (1H), 2.0-2.2 (2H),
1.6-1.8 (1H), 1.43 (1H), 1.26 (3H), 1.20 (3H), 0.81 (3H); .sup.13C
NMR (75 MHz, Acetone-d.sub.6) .delta. 177.64, 173.39, 172.92,
165.03, 147.96, 138.68, 134.68, 134.11, 133.81, 131.70, 130.52,
129.97, 125.18, 120.62, 120.54, 56.90, 54.55, 53.88, 52.31, 47.16,
37.56, 33.40, 23.48, 23.00, 22.30, 21.66; ESMS (m/z) 526
(MH.sup.+).
[0312] Example 101 (605 mg, 1.15 mmol) was dissolved in H.sub.2O (5
mL) containing LiOH (138 mg, 5.76 mmol). This was stirred for 4
hours. The solution thus obtained was acidified with 2 N HCl (10
mL) and the precipitate filtered. The filter cake was washed with
cold H.sub.2O (3 mL) and then dried under high vacuum to provide
Example 102 (539 mg, 92%) as a white amorphous powder:
[0313] .sup.1H NMR (300 MHz, DMSO-d.sub.6), .delta. 10.66 (1H),
8.14 (1H), 7.85 (2H), 7.76 (2H), 7.57 (2H), 7.22 (2H), 4.4-4.5
(1H), 3.02 (1H), 2.88 (1H), 2.70 (1H), 2.3-2.4 (1H) 1.8-2.0 (1H),
1.5-1.6 (1H), 1.2-1.4 (1H), 1.17 (3H) 1.12 (3H), 0.67 (3H);
.sup.13C NMR (75 MHz, DMSO-d.sub.6), .delta. 177.00, 173.17,
172.03, 163.90, 146.50, 137.19, 134.04, 133.35, 132.69, 130.92,
129.38, 129.28, 124.23, 119.44, 55.50, 53.59, 51.90, 45.95, 36.10,
32.23, 22.30, 22.23, 21.72, 21.00; ESMS (m/z) 512 (MH.sup.+).
78
Preparation 10-A
Scheme 15, 10-A Stereochemistry=(1R-cis)
[0314] (1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid
3-methyl ester 10-A (C.sub.11H.sub.18O.sub.4).
[0315] To methanol (0.5L), cooled in an ice-water bath under
nitrogen, is added acetyl chloride (50 mL, 0.703 mol) over 30
minutes. After stirring for 30 minutes (1R, 3S)-camphoric acid 15-A
(10 g, 0.5 mol) was added in one portion. The camphoric acid
dissolves over ca. 10 minutes and the solution was allowed to warm
slowly to room temperature and stir for 72 hours. Concentration in
vacuo afforded a clear, pale yellow oil which was dissolved in
ethyl acetate (0.6L). The solution was extracted with 0.5N aq. NaOH
(4.times.0.35L). The combined aqueous phases were washed with
pentane (0.35L) and the pH of the aq. layer was adjusted to ca. 4
with 1N aq. HCl. The aqueous layer was extracted with ether
(4.times.0.35L) and the combined organic phases were concentrated
in vacuo to give a colorless oil which slowly solidified to afford
96 g (90%) of 10-A as a white crystalline solid. An analytical
sample can be obtained by recrystallizing 10-A from ether-pentane
(1:1) to provide 10-A as clear hexagonal plates.
[0316] MP: 76-77.degree. C.; 1H-NMR (CDCl.sub.3) .delta. 3.69 (s,
3H), 2.79 (m, 1H), 2.54 (m, 1H), 2.20 (m, 1H), 1.84 (m, 1H), 1.54
(m, 1H), 1.45 (m, 1H), 1.27 (s, 3H), 1.26 (s, 3H), 0.86 (s, 3H); IR
(nujol): 3201, 2925, 1730, 1700, 1237, 1210, 1150, and 1110 cm-1;
Anal: Calcd. for C.sub.11H.sub.18O.sub.4: C, 61.66; H, 8.47; Found:
C, 61.63; H, 8.75.
Preparation 15-C
Scheme 15, 15-C Stereochemistry=(1R-cis)
[0317] (1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid 1
(1,1-dimethylethyl)-3-methyl diester 15-C
(C.sub.15H.sub.26O.sub.4)
[0318] To 10-A (25 g, 0.117 mol) in a 500 mL Parr bottle, cooled in
a dry ice-iPrOH bath under nitrogen, was condensed isobutylene
until the bottle was approximately 1/2 full. In a separate
Erlenmeyer flask, ether (6 mL) was cooled (dry ice-iPrOH bath) and
conc. sulfuric acid (3 mL) was added. The mixture was allowed to
cool for ca. 5 minutes, then was slowly added via disposable pipet
to the isobutylene-15-B mixture. The Parr bottle was transferred to
the shaker apparatus and shaken for 12 hours (pressure ca. 35 psi
at the end of 12 hours). The bottle was surrounded by aluminum
foil, dry ice was added to cool the bottle and contents, and the
bottle was removed from the shaker when the pressure reading was
ca. 0 psi. The isobutylene was condensed from the reaction vessel
via a cold finger condenser over ca. 2 hours. The resulting thin
oil was dissolved in pentane (0.5L), the organic phase was washed
with water (2.times.0.25L), 0.5N aq. NaOH (2.times.50 mL), and
water (2.times.0.25L). The organic phase was concentrated in vacuo
to provide 15-C as a clear colorless oil which slowly solidified at
room temperature. Recrystallization from petroleum ether gave 15-C
as a fine white crystalline solid (26.86 g, 89%).
[0319] MP: 36-37.6.degree. C.; .sup.1H-NMR: (300 MHz, CDCl.sub.3):
.delta. 3.68 (s, 31H) 2.78 (m, 1H), 2.52 (m, 1H), 2.16 (m, 1H),
1.78 (m, 1H), 1.45 (m, 1H), 1.45 (s, 9H), 1.24 (s, 3H), 1.17 (s,
3H), 0.81 (s, 3H).
Preparation 15-D
Scheme 15, 15-D Stereochemistry=(1R-cis)
[0320] (1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl) ester 15-D (C.sub.14H.sub.24O.sub.4)
[0321] To 15-C (10.25 g, 0.38 mol) in methanol (0.1L) was added in
order water (0.1L, see considerable precipitation), LiOH di-hydrate
(10 g, 2.38 mol), and 30% aq. hydrogen peroxide (0.1L,). The
mixture was warmed in a 90.degree. C. oil bath and allowed to stir
for 20 hours. The mixture was cooled to room temperature and
concentrated in vacuo to remove methanol and the residual white
solid material was removed by filtration. The clear solution was
washed with pentane (3.times.0.15L), the aqueous layer was cooled
in an ice-water bath and carefully acidified to ca. pH 4 with 1N
aq. HCl. The resulting white precipitate was isolated by
filtration, washed with water (3.times.0.1L) and air dried to
afford 15-D (9.6 g, 96%) as a fine white solid.
[0322] MP: 98-98.6.degree. C.; .sup.1H-NMR: (300 MHz, CDCl.sub.3):
.delta. 2.82 (1H) 2.50 (1H), 2.13 (1H), 1.79 (1H), 1.46 (9H) 1.45
(1H), 1.28 (3H), 1.18 (3H), 0.89 (3H); IR (nujol): 3075, 3025,
3006, 1719, 1689, 1270, 1249, 1164, and 851 cm.sup.-1; EI/MS: 200
(3.8), 183 (7.3), 164 (7.1), 154 (20.3), 136 (14.6), 109 (32.8), 57
(base); Anal: Calcd. for C.sub.14H.sub.24O.sub.4-0.18H.sub.2O: C,
64.77; H, 9.46; Found: C, 64.79; H, 9.44; K.F.-Water: 0.86%. 79
[0323] Boc-piperazine (1.9 gm, 10.4 mmol) was dissolved in THF (10
mL) and CH.sub.2Cl.sub.2 (1 mL) containing DIEA (5.6 mL, 32.2
mmol). To this solution 2,6-dichlorobenzoyl chloride (1.6 mL, 11.4
mmol) was added at 0.degree. C. After stirring the reaction for 1
hour at 0.degree. C., 1N HCl (30 mL) was added. The mixture was
extracted with CH.sub.2Cl.sub.2 (2.times.20 mL) and the combined
organics dried (Na.sub.2SO.sub.4), filtered and the solvent removed
in vacuo. Chromatography of the residue (SiO.sub.2, gradient
elution, hexanes.fwdarw.30% EtOAc/hexanes) provided Intermediate
(16-A) as an off white solid (3.6 gm, 98%): mp=157-159.degree. C.;
ESMS (m/z) 359 (MH.sup.+). 80
[0324] Intermediate 16-A (553 mg, 1.54 mmol) was dissolved in
CH.sub.2Cl.sub.2 (5 mL) and treated with TFA (5 mL) After 2 hours
the solvent was removed under reduced pressure. The residue was
dissolved in CH.sub.2Cl.sub.2 (20 mL) containing DIEA (0.8 mL, 4.6
mmol). Boc-aspartic acid .alpha. t-butyl ester (534 mg, 1.85 mmol)
was added with BOP-Cl (470 mg, 1.85 mmol). After stirring at RT for
24 hours the reaction was quenched with 1N HCl (25 mL). The mixture
was extracted with CH.sub.2Cl.sub.2 (3.times.25 mL). The combined
organics were dried (Na.sub.2SO.sub.4), filtered and the solvent
removed in vacuo. The residue was purified by column chromatography
(SiO.sub.2, gradient elution, hexanes.fwdarw.500 EtOAc/hexanes) to
provide the intermediate aspartate (17-A) (405 mg, 50%): ESMS (m/z)
530 (MH.sup.+).
[0325] To a methanolic solution of the above mentioned intermediate
17-A (0.2 g, 0.378 mmol), HCl gas was bubbled for 5 minutes and the
reaction mixture was left to stand overnight at room temperature.
The methanol was evaporated and the residual gum was triturated
with ether. The resultant solid was washed with ether and dried
under high vacuum. The solid was suspended in THF (5 mL), and 17-B
(1R,3S)-1-(tert-butoxycarbonyl)-1,2,2-t-
rimethylcyclopentanecarboxylic acid (102 mg, 0.397 mmol), BOP (176
mg, 0.397 mmol) were added, followed by DIEA (0.207 mL, 1.19 mmol).
The mixture was stirred overnight at room temperature. The solvent
was evaporated and the residue was partitioned between 1N HCl (5
mL) and EtOAc (15 mL). The organic layer was separated and washed
successively with 1N HCl (5 mL), brine (5 mL), Sat. NaHCO.sub.3
(2.times.5 mL), Sat. LiCl (2.times.5 mL), and dried over
MgSO.sub.4. Evaporation of EtOAc produced a colorless solid, which
was purified on silica (Chromatotron, hexane:EtOAc (1:1) as eluant)
to provide Example 176 (206 mg, 87%) as a colorless solid. ESMS:
(m/z) 626 (MH.sup.+).
[0326] Example 176 (0.18 g, 0.287 mmol) was dissolved in
CH.sub.2Cl.sub.2 (1.0 mL) and TFA (1.0 mL). After stirring at room
temperature for 1 hr the solvent was evaporated and the resultant
gum triturated with ether. The solid was washed with ether and
dried under vacuum to provide
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopen-
tyl)carbonyl]amino]-4-(2,6-dichlorobenzoyl)-.gamma.-oxo-1-piperazinebutano-
ic acid methyl ester as a colorless solid (0.14-g, 87%). ESMS (m/z)
570 (MH.sup.+).
[0327] To a solution of the methyl ester obtained above (0.1 g,
0.18 mmol) in THF/CH.sub.3OH (5 mL/1 mL) was added an aqueous (1
mL) solution of LiOH mono hydrate (19 mg, 0.378 mmol) and the
mixture was stirred at room temperature for 2 h. The organics were
evaporated and the residue was taken up in 2 ml of water and
acidified with citric acid. The solid was filtered, washed with
water and vacuum dried to provide Example 177 (90 mg, 92%). ESMS
(m/z) 556 (MH.sup.+). 81
[0328] Intermediate 16-A (593 mg, 1.54 mmol) was dissolved in
CH.sub.2Cl.sub.2 (6 mL) and treated with TFA (6 mL). After 2 hours
the solvent was removed under reduced pressure. The residue was
dissolved in CH.sub.2CL. (20 mL) containing DIEA (0.9 mL, 4.95
mmol). Boc-aspartic acid .beta. t-butyl ester (573 mg, 1.98 mmol)
was added with BOP-Cl (504 mg, 1.98 mmol). After stirring at RT for
24 hours the reaction was quenched with 1N HCl (15 mL). The mixture
was extracted with CH.sub.2Cl.sub.2 (3.times.20 mL). The combined
organics were dried (Na.sub.2SO.sub.4), filtered and the solvent
removed in vacuo. The residue was purified by column chromatography
(SiO.sub.2, gradient elution, hexanes.fwdarw.50% EtOAc/hexanes) to
provide the intermediate aspartate (18-A) (360 mg, 41 ESMS (m/z)
530 (MH.sup.+).
[0329] To a methanolic solution of the above mentioned intermediate
(18-A) (0.25 g, 0.47 mmol), HCl gas was bubbled for 5 minutes and
the reaction mixture was left to stand overnight at room
temperature. The methanol was evaporated and the residual gum was
triturated with ether. The resultant solid was washed with ether
and dried under high vacuum. The solid was suspended in THF (5 mL),
and 15-D (1R,3S)-1-(tert-butoxycarbonyl)-1,2,2-t-
rimethylcyclopentanecarboxylic acid (127 mg, 0.495 mmol), BOP (219
mg, 0.495 mmol) were added, followed by DIEA (0.259 mL, 1.485
mmol). The mixture was stirred overnight at room temperature. The
solvent was evaporated and the residue was partitioned between 1N
HCl (5 ml) and EtOAc (15 ml). The organic layer was separated and
washed successively with 1N HCl (5 ml), brine (5 ml), Sat.
NaHCO.sub.3 (2.times.5 ml), Sat. LiCl (2.times.5 ml), and dried
over MgSO.sub.4. Evaporation of EtOAc produced a colorless solid,
which was purified on silica (Chromatotron, hexane:EtOAc (1:1) as
eluant) to provide Example 178 (249 mg, 77%) as a colorless solid.
ESMS: (m/z) b26 (MH.sup.+).
[0330] Example 178 (0.19 g, 0.303 mmol) was dissolved in
CH.sub.2Cl.sub.2 (1.0 mL) and TFA (1.0 mL). After stirring at room
temperature for 1 hr the solvent was evaporated and the resultant
gum triturated with ether. The solid was washed with ether and
dried under vacuum to provide
[1S-[1.alpha.(R*),3.alpha.]]-.beta.-[[(3-Carboxy-2,2,3-trimethylcyclopent-
yl)carbonylamino]-4-[2,6-dichlorobenzoyl)-.gamma.-oxo-1-piperazinebutanoic
acid methyl ester as a colorless solid (98 mg, 58%). ESMS (m/z) 570
(MH.sup.+).
[0331] To a solution of the methyl ester obtained above (60 mg,
0.105 mmol) in THF/CH.sub.3OH (5 mL/1 mL) was added an aqueous (1
mL) solution of LiOH mono hydrate (9.5 mg, 0.221 mmol) and the
mixture was stirred at room temperature for 2 h. The organics were
evaporated and the residue was taken up in 2 ml of water and
acidified with citric acid. The solid was filtered, washed with
water and vacuum dried to provide Example 195 (48 mg, 836). ESMS
(m/z) 556 (MH.sup.+). 82
Preparation 19-C-2
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-2=H,
R.sup.19-1=CH.sub.3, R.sup.19-3=OC(CH.sub.3).sub.3, m=0
Stereochemistry=[1S-[1.alpha.,3.alpha.(S*)]]
[0332] [1S[1.alpha.,3.alpha.(S*)]]
N-3-[[(1-(1,1-dimethylethyoxy)carbonyle- thyl)
amino]carbonyl]-2,2,3-trimethylcyclopentyl)carbonyl-4-[(2,6-dichioro-
benzoyl)amino]-L-phenylalanine methyl ester (19-C-1)
(C.sub.34H.sub.43Cl.sub.2N.sub.3O.sub.7)
[0333] A solution of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)car-
bonyl]-4-[(2,6-dichiorobenzoyl)amino]-L-phenylalanine methyl ester
19-A-1 (19-A: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzo- yl)amino]phenyl) (0.468 g, 0.85
mmol), HATU (0.36 g, 0.95 mmol), and diisopropylethyl amine (dry, 2
mL, 11.5 mmol) are stirred together with DMF (dry, 5 mL) in a dry
round bottom flask under a nitrogen atmosphere. After 30 minutes,
D-alanine tbutyl ester hydrochloride (19-B, (R.sup.19-1=CH.sub.3,
R.sup.19-2=H, R.sup.19-3=t-BuO, Stereochemistry D) (0.34 g, 1.87
mmol) is added. After three days, the mixture is evaporated to
dryness in vacuo to give a yellow oil which is mixed with methylene
chloride (100 mL) and shaken with water (5.times.50 mL). The
organic layer is then evaporated to dryness, giving an off-white
solid Recrystallization from ethyl acetate/diethyl ether gives
19-C-1, as a white solid (0.428 g, 74% yield). .sup.1H NMR (300
MHz, DMSO-d.sub.6) .delta. 10.70 (1H), 7.90 (1H), 7.60-7.18 (8H),
4.48 (1H), 4.15 (1H), 3.57 (3H), 2.95 (2H), 2.75 (1H), 2.37 (1H),
1.87 (1H), 1.55 (1H), 1.36 (9H), 1.30 (1H), 1.22 (3H), 1.18 (3H),
1.09 (3H), 0.57 (3H).
Preparation 19-C-2
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl R.sup.19-2=H,
R.sup.19-1=CH.sub.3, R.sup.19-3=OH, m=0
Stereochemistry=[1S-[1.alpha.,3.a- lpha.(S*)]]
[0334] [1S-[1.alpha.,3.alpha.(S*)]
J-N-[[3-[(1-Carboxyethyl)amino]carbonyl-
]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine methyl ester (19-C-2)
(C.sub.30H.sub.35Cl.sub.2N.sub.3O.sub.7)
[0335] Di-ester 19-C-1 is stirred overnight with trifluoroacetic
acid (5 mL) and then the mixture is diluted with toluene (100 mL)
followed by evaporation to dryness in vacuo, giving acid 19-C-2 as
a pale brown oil (0.34 g, 90% yield).
[0336] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 10.70 (1H), 7.96
(1H), 7.57-7.10 (8H), 4.47 (1H), 4.22 (1H), 3.57 (3H), 2.95 (2H),
2.65 (1H), 2.37 (1H), 1.89 (1H), 1.55 (1H), 1.30 (1H), 1.25 (3H),
1.18 (3H), 1.09 (3H), 0.57 (3H); MS (ES+) m/z 619.8.
Preparation of Example 211
Scheme 19, 19-D: wherein R.sup.4=H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]p- henyl R.sup.19-2=H,
R.sup.19-1=CH.sub.3, R.sup.19-3=OH, m=0
Stereochemistry=[1S-[1.alpha.,3.alpha.(S*)]]
[0337]
[1S-[1.alpha.,3.alpha.(S*)]]-N-[[3-[[(1-Carboxyethyl)amino]carbonyl-
]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine Example 211
(C.sub.29H.sub.33Cl.sub.2N.sub.3O.sub.7)
[0338] A solution of LiOH.sub.12O (0.15 g, 3.57 mm) in H.sub.2O (5
mL) is added to a solution of 19-C-2 in methanol (5 mL). After
overnight stirring, the solution is brought to pH 7 with 1.2N aq.
HCl (2 mL), evaporated in vacuo until the methanol is gone, and
shaken with diethyl ether (3.times.30 mL). The aqueous layer is
filtered, cooled in an ice bath and brought to pH 2 using 1.2N aq.
HCl. The resultant white precipitate is filtered, washed with water
(100 mL) and air dried to give Example 211 as a white solid (0.277
g, 78% yield).
[0339] .sup.1H NMR (DMSO-d.sub.6) .delta. 10.67 (1H), 7.76 (1H),
7.57-7.44 (5H) 7.31 (1H), 7.20 (2H), 4.42 (1H), 4.22 (1H),
3.03-2.82 (2H), 2.64 (1H), 2.36 (1H), 1.89 (1H), 1.54 (1H)
1.32-1.26 (4H), 1.19 (3H), 1.09 (3H), 0.58 (3H); IR (nujol) 3293,
3261, 3078, 1740, 1672, 1612, 1562, 1551, 1527, 1518, 1429, 1415,
1334, 1276, 1197 cm.sup.-1; MS (ES-) m/z 606.3, 604.3; KF Water
7.76%.
Preparation 19-C-3
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5 CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-2,
R.sup.19-1=(CH.sub.2) 3, R.sup.19-3=NH.sub.2, m=0
Stereochemistry=[1S-[1.- alpha.,3.alpha.(S*)]]
[0340]
[1S-[1.alpha.,3.alpha.(S*)]]-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidiny-
l]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)a-
mino]-L-phenylalanine methyl ester (19-C-3)
(C.sub.32H.sub.37Cl.sub.2N.sub- .4O.sub.6)
[0341] In a similar manner to that reported for the synthesis of
19-C-1, 19-A-1 (19-A: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl))(0.55 g, 1.00 mmol)
is coupled to D-prolineamide (0.3 g, 2.63 mmol) using HATU (0.40 g,
1.05 mmol) and diisopropylethyl amine (3 mL, 17.2 mmol) in dry DMF.
After overnight stirring, the reaction mixture is evaporated to
dryness in vacuo, giving a pale yellow oil which is stirred with
ethyl acetate (50 mL). A precipitate soon formed and it is isolated
by suction filtration to give 19-C-3 as a white solid (0.632 g, 900
yield).
[0342] .sup.1H NMR (DMSO-d.sub.6) .delta. 10.67 (1H), 7.95 (1H),
7.58-7.48 (5H), 7.19 (2H), 7.10 (1H), 6.65 (1H), 4.45 (1H), 4.18
(1H), 3.70-3.30 (1H), 3.57 (3H), 2.90 (2H), 2.58 (111), 2.28-1.40
(9H), 1.25 (3H), 1.12 (3H), 0.73 (3H).
Preparation of Example 212
Scheme 19, 19-D: wherein R.sup.4=H, R.sup.5=CO.sub.2Li
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-2,
R.sup.19-1=(CH.sub.2) 3, R.sup.19-3=NH.sub.2, m=0
Stereochemistry=[1S-[1.- alpha.,3.alpha.(S*)]]
[0343]
[1S-[1.alpha.,3.alpha.(S*)]]-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidiny-
l]carbonyl]-2,2,3-trimethylcyclopentyl]-carbonyl]-4-[(2,6-dichlorobenzoyl)-
amino]-L-phenylalanine monolithium salt Example 212
(C.sub.31H.sub.35Cl.sub.2N.sub.4O.sub.6Li)
[0344] A solution of LiOH.H.sub.2O (0.2 g, 4.8 mmol) in H.sub.2O (4
mL) is added to a stirred solution of the methyl ester 19C-3 (0.58
g, 0.89 mmol) in methanol (10 mL). After overnight stirring, the
mixture is evaporated in vacuo until the methanol is gone. The
reaction mixture is then brought to pH 7 with 1N aq. HCl, filtered
and the filtrate transferred to a C-18 reversed phase HPLC column
and eluted with a 0-10% acetonitrile/water gradient. Evaporation is
accomplished in vacuo to give the target compound as a white solid
(0.445 g, 78% yield).
[0345] .sup.1H NMR (300 MHz, DMSO-d,) .delta. 10.55 (1H), 7.56-7.43
(5H), 7.04 (3H), 6.79 (1H), 6.62 (1H), 4.18 (1H), 3.92 (1H) 3.62
(1H) 3.43 (1H), 2.96 (2H), 2.44 (1H), 2.25-1.5 (8H) 1.23 (3H), 1.1
(3H), 0.75 (3H); IR (nujol) 3392, 3288, 3194, 3124, 3068, 1660,
1604, 1562, 1539, 1515, 1431, 1403, 1325, 799, 686 cm.sup.-1; MS
(FAB) m/z (rel. intensity) 631 (M+H, 1), 659 (29), 653 (24), 643
(26), 639 (34), 637 (50), 279 (33), 133 (26), 109 (61), 70 (35), 23
(99); HRMS (FAB) m/z calcd for
C.sub.31H.sub.36Cl.sub.2N.sub.4O.sub.6+H.sup.+ 631.2090, found
631.2086; KF Water: 9.90%.
Preparation 19-C-4
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl, R.sup.19-2,
R.sup.19-1=(CH.sub.2) 3, R.sup.19-3=NH.sub.2, m=0
Stereochemistry=[1S-[1.- alpha.,3.alpha.(R*)]]
[0346]
[1S-[1.alpha.,3.alpha.(R*)]]-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidiny-
l]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)a-
mino]-L-phenyl alanine methyl ester (19-C-4)
(C.sub.32H.sub.37Cl.sub.2N.su- b.4O.sub.6)
[0347] In a similar manner to that reported for the synthesis of
19-C-3,19-A-1 (19-A: R.sup.4=H, R.sup.5 CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl))(0.50 g, 0.91 mmol)
is coupled to L-prolineamide (0.2 g, 1.75 mmol) using HATU (0.41 g,
1.08 mmol) and diisopropylethyl amine (1 mL, 5.74 mmol) in dry DMF
(5 mL). After overnight stirring, the mixture is evaporated to
dryness in vacuo to give a yellow oil which is shaken with
methylene chloride (100 mL) and water (50 mL); this gives a white
precipitate which is filtered and the isolated solid is washed with
water (3.times.50 mL). Recrystallization from ethyl acetate gives
19-C-4 as a white solid (0.31 g, 53% yield).
[0348] .sup.1H NMR (DMSO-d): .delta. 10.70 (1H), 7.92 (1H), 7.50
(5H) 7.19 (2H), 7.04 (1H), 6.74 (1H), 4.48 (1H), 4.11 (1H), 3.58
(3H), 3.47 (1H), 2.92 (2H), 2.56 (1H), 2.35 (1H), 2.10-1.45 (8H),
1.24 (3H), 1.09 (3H), 0.71 (3H); MS (ES+, m/z) 644.9.
Preparation of Example 213
Scheme 19, 19-D: wherein R.sup.4=Hi R.sup.5=CO.sub.2Na
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl, R.sup.19-2,
R.sup.19-1=(CH.sub.2).sub.3, R.sup.19-3=NH.sub.2, In=0
Stereochemistry=[1S-[1.alpha.(S*),3.alpha.(S*)]]
[0349]
[1S-[1.alpha.,3.alpha.(R*)]]-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidiny-
l]carbonyl]-2,2,3-trimethylcyclopentyl]-carbonyl]-4-[(2,6-dichlorobenzoyl)-
amino]-L-phenylalanine monosodium salt Example 213
(C.sub.31H.sub.35Cl.sub- .2N.sub.4O.sub.6Na)
[0350] The ester 19-C-4 (0.258 g., 0.4 mmol) is hydrolyzed in a
manner similar to that described above for Example 212. The
reaction mixture is then brought to pH 7, filtered and the filtrate
transferred to a C-18 reversed phase HPLC column and eluted with a
0-10% acetonitrile/0.01 aqueous Na.sub.2CO.sub.3 gradient.
Evaporation is accomplished in vacuo to give the target compound as
a white solid (0.2 g, 76% yield).
[0351] .sup.1H NMR (300 MHz, DMSO-d,): .delta. 10.57 (1H),
7.56-7.44 (4H), 7.02 (3H), 6.77 (2H), 4.10 (1H), 3.91 (1H), 3.6
(1H), 3.50 (1H), 3.95 (1H), 2.44 (1H), 2.10-1.50 (7H), 1.23 (3H),
1.07 (3H), 0.73 (3H); IR (nujol) 3392, 3288, 3194, 3124, 3068,
1660, 1604, 1562, 1539, 1515, 1431, 1403, 1325, 799, 686 cm.sup.-1;
MS (FAB) m/z (rel. intensity) 631 (M+H, 1), 659 (29), 653 (24), 643
(26), 639 (34), 637 (50), 279 (33), 133 (26), 109 (61), 70 (35), 23
(99); HRMS (FAB) calcd for C.sub.31H.sub.36Cl.sub.2-
N.sub.4O.sub.6+H.sup.+ 631.2090, found 631.2086; KF Water:
9.90%.
Preparation 19-C-5
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-2=H,
R.sup.19-1=H, R.sup.19=OCH.sub.3, m=1
Stereochemistry=(1S-cis)-L
[0352]
(1S-cis)-N-[[3-[[[2-(Methyloxycarbonyl)ethyl]amino]carbonyl]-2,2,3--
trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalan-
ine methyl ester (19-C-5)
(C.sub.31H.sub.37Cl.sub.2N.sub.3O.sub.7)
[0353] A solution of 19-A-1 (19-A: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.6 g, 1.09 mmol),
HATU (0.5 g, 1.32 mmol), and diisopropylethyl amine (dry, 2 mL,
11.5 mmol) are stirred together with DMF (dry, 5 mL) in a dry round
bottom flask under a nitrogen atmosphere. After 30-minutes,
O-alanine methyl ester hydrochloride (19-B, R.sup.19-1=H,
R.sup.19-2=H, R.sup.19-3=CH.sub.3O) (0.3 g, 2.15 mmol) is added.
After 18 hours, the mixture is evaporated to dryness in vacuo to
give a yellow oil which is mixed with ehtyl acetate (150 mL) and
shaken with water (50 mL). The organic layer is washed with water
and brine (2.times.60 mL, 5:1), satd aq. NaHCO.sub.3 (50 mL), water
and brine (60 mL, 5:1), the organic layer is mixed with pentane (30
mL) and is cooled to -20.degree. C. (3 days). The resulting solid
is isolated by filtration, washed with diethyl ether (2.times.50
mL), and is air dried to give 19-C-5 as a white solid (0.63 g,
91%).
[0354] .sup.1H NMR (CDCl.sub.3) .delta. 7.62 (1H), 7.57 (2H), 7.32
(3H), 7.10 (2H), 6.26 (1H), 5.81 (1H), 4.88 (1H), 3.75 (3H), 3.68
(3H), 3.48 (2H), 3.12 (2H), 2.51 (3H), 2.28 (2H), 1.80 (1H), 1.49
(1H), 1.26 (3H), 1.15 (3H), 0.74 (3H); MS (ES+) m/z 633.8.
Preparation of Example 214
Scheme 19, 19-D: wherein R.sup.4=H,
R.sub.6=4-[(2,6-Dichlorobenzoyl)amino]- phenyl, R.sup.19-2=H,
R.sup.19-1=H, R.sup.19-3=OH, m=1 Stereochemistry=(1S-cis)-L
[0355]
(1S-cis)-N-[[3-[[(2-Carboxyethyl)amino]carbonyl]-2,2,3-trimethylcyc-
lopentyl ]carbonyl]-4-[[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(Example 214) (C.sub.29H.sub.33Cl.sub.2N.sub.3O.sub.7)
[0356] To a solution of the dimethyl ester 19-C-5 (19-C: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl, R.sup.19-2=H,
R.sup.19-1=H, R.sup.19-3=OCH.sub.3, n=1,
Stereochemistry=(1S-cis)-L) (0.52 g, 0.82 mmol) in methanol (10 mL)
is added a solution of LiOH.H.sub.2O (0.18 g, 4.29 mmol) in water
(4 mL). After overnight stirring, the reaction mixture is brought
to pH 8 using 1N aq. HCl and then evaporated to dryness in vacuo.
The mixture is chromatographed on a reversed phase (C-18) HPLC
column using a gradient (0 to 10% acetonitrile/(36 methanol in
H.sub.2O)). The selected eluant is evaporated to dryness and
dissolved in water (50 mL), cooled in an ice water bath, and
brought to pH 3 using 1N aq. HCl. The resultant white precipitate
is isolated by suction filtration to give Example 214 as a white
solid (0.43 g, 85% yield).
[0357] .sup.1H NMR (DMSO-d.sub.6) .delta. 10.6 (1H), 7.91 (1H),
7.55-7.42 (5H) 7.08 (2H), 6.78 (1H), 4.02 (1H), 3.10 (2H), 2.90
(2H), 2.48 (1H), 2.25 (1H), 1.93 (3H), 1.56 (1H), 1.24 (1H), 0.99
(3H), 0.93 (3H), 0.46 (3H); IR (nujol) 3327, 3080, 1726, 1672,
1622, 1614, 1595, 1558, 1515, 1429, 1338, 1279, 1262, 1197, 783
cm.sup.-1; MS (FAB) m/z (rel. intensity) 606 (M+H, 99), 609 (24),
608 (69), 607 (42), 606 (99), 605 (15), 517 (16), 254 (691, 175
(16), 173 (25), 109 (51); HRMS (FAB) m/z calcd for
C.sub.29H.sub.33Cl.sub.2N.sub.3O.sub.7+H1 606.1774, found
606.1758.
Preparation 19-C-6
Scheme 19, 19-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-2,
R.sup.19-1=(CH.sub.2).sub.3, R.sup.19-3=NHCH.sub.3, m=0
Stereochemistry=[1S-[1.alpha.,3.alpha.(R*)]]
[0358]
[1S-[1.alpha.,3.alpha.(R*)]]-4-[(2,6-Dichlorobenzoyl)amino]-N-[3-[[-
2-[(methylamino)carbonyl]-1-pyrrolidinyl]carbonyl]-2,2,3-trimethylcyclopen-
tyl]carbonyl]-L-phenylalanine methyl ester (19-C-6)
(C.sub.33H.sub.40Cl.sub.2N.sub.4O.sub.6)
[0359] In a similar manner to that reported for the synthesis of
19-C-1,19-A-1 (19-A: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl) (0.53 g, 0.96 mmol),
HATU (0.4 g, 1.05 mmol), and diisopropylethyl amine (dry, 1.5 mL,
8.6 mmol) are stirred together with DMF (dry, 5 mL) in a dry round
bottom flask under a nitrogen atmosphere. After 30 minutes,
N-methylprolineamide hydrochloride (19-B: R.sup.19-1,
R.sup.19-2=CH.sub.2 CH.sub.2CH.sub.2, R.sup.19-3=NHCH.sub.3, n=0,
stereochemistry L) (0.5 g, 3.04 mmol) is added. After two days, the
mixture is evaporated to dryness in vacuo to give a pale brown oil
which is transferred to a silica gel column (20 g) and eluted with
a gradient from 0 to 10% methanol/chloroform to give, after solvent
evaporation, 19-C-6 as a pale oil (0.55 g, 86%)
[0360] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 10.68 (1H), 7.95
(1H), 7.58-7.44 (7H), 7.19 (2H), 4.48 (1H), 4.11 (2H), 3.57 (3H),
3.06 (4H), 2.63 (3H), 2.46-1.69 (11H), 1.31 (3H), 1.12 (3H), 0.79
(3H).
Preparation of Example 215
Scheme 19, 19-D: wherein R.sup.4=H, R.sup.5=CO.sub.2Na
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.19-1,
R.sup.19-2 CH;CH.sub.2CH.sub.2, R.sup.19-3=NHCH.sub.3, m=0
Stereochemistry [1S-[1.alpha.,3.alpha.(R*)]]
[0361]
[1S-[1.alpha.,3.alpha.(R*)]]-N-[3-[[2-[(Methylamino)carbonyl]-1-pyr-
rolidinyl]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorob-
enzoyl)amino]-L-phenylalanine monosodium salt Example 215
(C.sub.32H.sub.37Cl.sub.2NaN.sub.4O.sub.6)
[0362] A solution of LiOH.H.sub.2O (0.2 g, 4.8 mmol) in H.sub.2O (6
mL) is added to a stirred solution of the methyl ester 19-C-6
(19-C: R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[2,6-Dichlorobenzoyl)amino]phenyl) (0.53 g, 0.8 mmol) in
methanol (10 mL). After overnight stirring, the mixture is diluted
with H.sub.2O (50 mL), evaporated in vacuo until the methanol is
gone. The reaction mixture is then brought to pH 8 using 1N aq.
HCl, filtered, and the filtrate transferred to a C-18 reversed
phase HPLC column and eluted with a 10'-18 acetonitrile/0.02% aq
sodium bIcarbonate gradient. The eluant is evaporated to dryness,
stirred with isopropanol (3.times.10 mL) and filtered through a
sintered glass funnel. The combined isopropanol filtrates are
evaporated to dryness, mixed with water and evaporated to dryness
again (2.times.10 mL H.sub.2O) to give Example 215 as a white solid
(0.4 g, 74% yield).
[0363] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 10.57 (1H), 7.51
(6H), 7.04 (2H), 6.80 (1H), 4.18 (1H), 3.90 (1H), 3.55 (2H), 3.00
(2H), 2.53 (3H), 2.47 (1H), 2.12-1.50 (8H), 1.24 (3H), 1.09 (3H),
0.72 (3H).
[0364] HRMS (FAB) m/z calcd for
C.sub.32H.sub.37CL.sub.2N.sub.4O.sub.6NaI +HI 667.2066; found
667.2056. 83
[0365] (1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid
1-methyl ester (20-B)
[0366] A solution of (1R)-camphoric anhydride, prepared by the
method of Bell, K. H. Aust. J. Chem. 1981, 34 665-670, (5 g, 27.44
mmol) in toluene (400 mL) in a round bottom flask is flushed with
N, and then cooled in a dry ice/ethanol bath. To this cooled
solution is added (via dropwise addition) a one molar solution of
potassium t-butoxide (30 mL, 30 mmol) over a twenty minute period.
After stirring overnight, the room temperature reaction mixture is
again cooled on a dry ice/ethanol bath. To the cooled solution is
added methyl trifluoromethanesulfonate (3.5 mL, 30.9 mmol). After
an additional 12 hours of stirring, the reaction mixture is
acidified with trifluoroacetic acid (50 mL, 649 mmol), and is
allowed to stir for another 16 hours. The mixture is then diluted
with toluene (200 mL), shaken with water (4.times.120 mL), and
evaporated to dryness, giving 20-B as a pale brown solid (2.35 g,
10.97 mmol, 40% yield).
[0367] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 3.60 (s, 3H),
2.75 (m, 1H) 2.40 (m, 1H), 2.00 (m, 1H), 1.74 (m, 1H), 1.41 (m,
1H), 1.19 (s, 3H), 1.15 (s, 3H), 0.70 (s, 3H).
Preparation 20-D
Scheme 20, 20-D: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorophenyl) methoxy]-phenyl
Stereochemistry=[1S-cis]-- L
[0368]
(1S-cis)-O-[(2,6-Dichlorophenyl)methyl]-N-[[3-(methoxycarbonyl)-2,2-
,3-trimethylcyclopentyl]-carbonyl]-L-tyrosine methyl ester
(20-D)
[0369] A solution of camphoric acid methyl ester 20-B (0.35 g, 1.63
mmol), EDC (0.35 g, 1.83 mmol), HOBT (0.25 g, 1.85 mmol),
4-dimethylaminopyridine (0.05 g, 0.41 mmol), in methylene chloride
(10 mL) is stirred together in a 25 mL, 2-neck flask cooled in an
ice water bath. To this mixture is added
(2,6-dichlorophenyl)methyl)-L-tyrosine methyl ester hydrochloride
(0.65 g, 1.83 mmol), creating a thick, heterogeneous mixture which
becomes homogeneous after addition of triethylamine (0.3 mL, 2.15
mmol). After three days, the reaction mixture is diluted with
methylene chloride (150 mL) and shaken with water (2.times.100 mL),
aqueous HCl (0.5N, 2.times.100 mL), water (3.times.100 mL), aqueous
NaHCO.sub.3 (2.times.100 mL) and water (1.times.100 mL). The
organic layer is then evaporated to dryness, giving 20-D as an
off-white foam (0.7 g, 78% yield).
[0370] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.38 (m, 2H), 7.26
(m, 1H), 7.05 (m, 2H), 6.96 (m, 2H), 5.76 (m, 1H), 5.25 (s, 2H),
4.88 (m, 1H), 3.75 (s, 3H), 3.67 (s, 3H), 3.17-3.03 (m, 2H),
2.61-2.50 (m, 2H), 2.26-2.13 (m, 1H), 1.84-1.70 (m, 1H), 1.54-1.45
(m, 1H), 1.22 (s, 3H), 1.19 (s, 31H), 0.76 (s, 3H), MS (ES+) m/z
549.8 84
[0371]
(1R-cis)-N-[[3-Methoxycarbonyl-1,2,2-trimethylcyclopentyl]carbonyl--
L-proline 1,1-dimethylethyl ester 21-A
[0372] The camphoric acid mono ester 10-A (1.41 g, 6.58 mmol) in
DMF (dry, 5 mL) in a dry flask under N2 is cooled in an ice water
bath. Diisopropylethyl amine (4.6 mL, 26.41 mmol) is added followed
by HATU (2.6 g, 6.83 mmol). After thirty minutes, L-proline t-butyl
ester (1.3 mL, 7.44 mmol) is added. After overnight stirring, the
mixture is evaporated to dryness, to give a pale yellow oil.
Recrystallization from CHCl.sub.3 to give 21-A as a white solid
(1.6 g, 66% yield). .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 4.32
(1H), 3.67 (3H), 3.59 (2H), 2.71 (1H), 2.39 (1H), 2.20 (2H),
2.10-1.70 (5H), 1.43 (9H), 1.38 (3H)l 1.20 (3H), 0.93 (3H); IR
(nujol) 1741, 1733, 1617, 1430, 1396, 1359, 1343, 1218, 1202, 1185,
1174, 1157, 1127, 1014, 771 cm.sup.-1; MS (FAB) m/z (rel.
intensity) 368 (M+H, 99), 369 (22), 368 (99), 312 (15), 280 (12),
197 (72), 169 (23), 137 (29), 109 (38), 70 (11), 57 (14). Anal.
Calcd for C.sub.20H.sub.33NO.sub.5: C, 65.37; H, 9.05; N, 3.81.
Found: C, 65.53; H, 8.88; N, 3.83.
[0373]
(1R-cis)-[3-Carboxy-1,2,2-trimethylcyclopentyl]carbonyl-L-proline
1,1-dimethylethyl ester 21-B
[0374] To a solution of the diester 21-A (0.74 g, 2.01 mmol) in
methanol (5 mL) is added a solution of LiOH.H.sub.2O (0.15 g, 3.62
mmol) in aq H.sub.2O.sub.2 (306, 2 mL) and water (5 mL). After
overnight stirring, the mixture is evaporated until all of the
methanol is gone, and then cooled in an ice water bath and brought
to pH 5 using 1N aq. HCl. The resultant white precipitate is
isolated by suction filtration (with water washes, 3.times.30 mL)
to give 21-B as a white solid (0.45 g, 63% yield).
[0375] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 4.34 (1H), 3.60
(2H), 2.75 (1H) 2.41 (1H), 2.18 (1H), 2.00-1.70 (5H), 1.44 (9H),
1.43 (3H), 1.22 (3H), 1.02 (3H).
[0376]
[1R-[1.alpha.,3.alpha.(S*)]]-N-[[3-[[[1-Carbomethoxy-2-[4-[(2,6-dic-
hlorophenyl)methoxy]phenyl]ethyl]amino]carbonyl]-1,2,2-trimethylcyclopenty-
l]carbonyl]-L-proline 1,1-dimethylethyl ester 21-C
[0377] A solution of the acid 21-B (0.41 g, 1.16 mmol) in methylene
chloride (20 mL) under N.sub.2 is cooled in an ice water bath. To
this is added diisopropylethyl amine (2 mL), EDC (0.26 g, 1.36
mmol), HOBT (0.19 g, 1.41 mmol) and dimethylaminopyridine (0.02 g,
0.16 mmol). Forty minutes later, 7-E-2 (7-E. R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=0.4-[(2,6-Dichlorophenyl)methoxy)phenyl) (0.45 g, 1.27
mmol) is added in one portion. After three days, the mixture is
evaporated to dryness, giving a colorless oil which is mixed with
THF (100 mL) and water (50 mL) and then shaken sequentially with
water (2.times.50 mL), aq. HCl (0.5N, 4.times.30 mL), satd. aq.
NaHCO.sub.3 (2.times.50 mL), and water (2.times.50 mL). The organic
layer is then evaporated to dryness, giving a colorless oil (0.91
g) which is chromatographed on silica gel with 10%
methanol/chloroform to give 21-C as a white, foamy solid (0.57 g,
70% yield).
[0378] .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 7.31-6.87 (7H),
5.80 (1H) 5.18 (2H), 4.81 (1H), 4.25 (1H), 3.67 (3H), 3.53 (2H),
3.04 (2H), 2.44-1.58 (9H), 1.37 (9H), 1.33 (3H), 1.13 (3H), 0.90
(3H). IR (nujol) 1739, 1658, 1622, 1612, 1585, 1511, 1439, 1298,
1241, 1204, 1177, 1153, 1122, 1017, 768 cm.sup.-1; MS (FAB) m/z
(rel. intensity) 689 (M+H, 24), 689 (24), 520 (69), 519 (32), 518
(99), 336 (44), 280 (21), 161 (24), 159 (40), 109 (75), 57
(22).
Preparation of Example 200
[0379]
[1R-[1.alpha.,3.alpha.(S*)]]-N-[[3-[[[1-Carboxy-2-[4-[(2,6-dichloro-
phenyl)methoxy]phenyl]ethyl]amino]carbonyl]-1,2,2-trimethylcyclopentyl]car-
bonyl]-L-proline 1,1-dimethylethylester, monosodium salt (Example
200)
[0380] To a solution of 21-C (0.43 g, 0.62 mmol) in methanol is
added a solution of LiOH.H.sub.2O (0.083 g, 1.98 mmol) in aqueous
H.sub.2O.sub.2 (30%, 3 mL) plus H.sub.2O (3 mL) After overnight
stirring, the mixture is diluted with water (50 mL) and evaporated
in vacuo until the methanol is gone. The aqueous layer is then
washed with diethyl ether (3.times.30 mL) and brought to pH 6 using
1 N aq. HCl. The resultant white precipitate is isolated by suction
filtration to give a white solid (0.4 g). This is stirred overnight
with NaHCO.sub.3 (0.1 g, 1.2 mmol) in H.sub.2O (5 mL). The aqueous
solution is brought to pH 7-8 using 1 N HCl and then transferred to
a C-18 HPLC column and eluted with a gradient of 0-12%
acetonitrile/aq. Na.sub.2CO.sub.3 (0.02%). Evaporation is
accomplished in vacuo to give
[1R-[1.alpha.,3.alpha.(S*)]]-N-r[3-[[[1-Carboxy-2-[4-[(2,6--
dichlorophenyl)methoxy]
phenyl]ethyl]amino]carbonyl]-1,2,2-trimethylcyclop-
entyl]carbonyl]-L-proline 1,1-dimethylethylester, monosodium salt
as a white solid (0.2 g, 460%)
[0381] .sup.1H NMR (DMSO-d.sub.6) .delta. 7.55-7.41 (3H), 7.01
(2H), 6.82 (2H), 6.80 (1H), 5.13 (2H), 4.08 (1H), 3.90 (1H), 3.65
(1H), 3.40 (1H), 3.31 (3H), 2.95 (2H), 2.44 (1H), 2.25-1.5 (8H),
1.34 (9H), 1.23 (3H), 1.08 (3H), 0.76 (3H), IR (nujol) 3405, 1735,
1610, 1565, 1511, 1439, 1299, 1240, 1195, 1175, 1153, 1093, 1018,
779, 769 cm.sup.-1; MS (FAB) m/z (rel. intensity) 675 (M+H, O), 720
(27), 701 (25), 700 (67), 699 (39), 698 (99), 336 (32), 280 (23),
159 (20), 109 (53), 23 (28). HRMS (FAB) m/z calcd for
C.sub.35H.sub.4Cl.sub.2N.sub.2O.sub.7 +Na 697.2424, found 697.2418.
85
Preparation 22-C-1
Scheme 22, 22-C: wherein R.sup.4=H R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
Stereochemistry=(1S-cis)-L
[0382]
(1S-cis)-N-[[3-[[(2-Cyanoethyl)amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (22-C-1) (C.sub.30H.sub.34Cl.sub.2N.sub.4O.sub.5)
[0383] A solution of 19-A-1 (19-A: R.sup.4=Hi
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.5 g, 0.91 mmol),
HOBT (0.12 g, 0.91 mmol), and DCC (0.18 g, 0.91 mmol are stirred
together in CH.sub.2Cl.sub.2 (dry, 10 mL) in a dry round bottom
flask under a nitrogen atmosphere. After 30 minutes, 2-cyanoethyl
amine 22-B (0.064 g, 0.91 mmol) is added. After 24 hours a
precipitate is observed and methanol (5 mL) is added to achieve a
homogenous solution and an additional portion of 2-cyanoethyl amine
22-B (0.064 g, 0.91 mmol) is added. After an additional 8 days of
stirring the solvent is removed in vacuo and the residue is
dissolved in THF and purified by chromatography on a column of
silica gel to give 22-C-1 (0.42 g, 78%) as a white solid.
[0384] .sup.1H-NMR (300 MHz, MeOH-d.sub.4): .delta. 7.58 (2H), 7.43
(3H) 7.22 (2H), 5.48 (1H), 4.72 (1H), 3.69 (3H), 3.58 (2H), 3.29
(1H), 3.02 (1H), 2.65-2.77 (3H), 2.40 (1H), 2.04 (1H), 1.76 (1H),
1.47 (1H), 1.27 (3H), 1.20 (3H), 0.74 (3H); Anal. Calcd for
C.sub.30H.sub.34Cl.sub.2N.sub- .4O.sub.7-0.54H.sub.2O: C, 58.95; H,
5.78; N, 9.17; Found: C, 59.04; H, 5.75; N, 9.22; KF Water
1.59%.
Preparation of Example 216
Scheme 22, 22-D: wherein R.sup.4=H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]p- henyl,
Stereochemistry=(1S-cis)-L
[0385]
(1S-cis)-N-[[3-[[(2-Cyanoethyl)amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(Example 216) (C.sub.29H.sub.32Cl.sub.2N.sub.4O.sub.5)
[0386] To the L-phenylalanine methyl ester 22-C-1 (22-C: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.16 g, 0.266 mmol),
in methanol (10 mL) is added a mixture of LiOH.H.sub.2O (0.056 g,
1.33 mmol), in H.sub.2O (3 mL). The mixture is allowed to stir at
room temperature for 2 hours, then the solvent is removed in vacuo.
The resulting solid is dissolved in water (10 mL) and the pH is
adjusted to ca. 2 with 1N aq. HCl to give a white precipitate the
precipitate is isolated by filtration, washed with water (10 mL)
and then dissolved in acetonitrile (25 mL). The organic phase is
dried (Na.sub.2SO.sub.4) and the solvent is removed in vacuo to
give a sticky solid which is dissolved in acetonitrile/water (25
mL, 1:3) and lyophilized to give 0.104 g (67%) of the target
compound as a white solid.
[0387] .sup.1H-NMR (300 MHz, MeOH-d.sub.4): .delta. 7.82 (1H), 7.57
(2H), 7.46 (3H), 7.24 (2H), 4.72 (1H), 3.40 (2H), 3.30 (1H), 2.99
(1H), 2.63-2.77 (3H), 2.40 (1H), 2.03 (1H), 1.74 (1H), 1.48 (1H),
1.27 (3H), 1.20 (3H), 0.75 (3H); IR (nujol): 3317, 3262, 1762,
1673, 1638, 1608, 1546, 1515, 1432, 1325, 1203, 811, 801, 780
cm.sup.-1; MS (FAB) m/z (rel. intensity) 587 (M.sup.+, base), 517
(12.4), 335 (9.8), 252 (8.5), 235 (80); Arial. Calcd for
C.sub.29H.sub.32Cl.sub.2N.sub.4O.sub.7.2.14H.sub.2- 0 : C, 55.64;
H, 5.84; N, 8.95; Found: C, 55.74; H. 5.72; N, 8.99; KF Water
6.16%. 86
Preparation 23-C-1
Scheme 23, 23-C: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
Stereochemistry=(1S-cis)-L
[0388]
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[2,2,3-trimethyl-3-[[(ph-
enylmethoxy)amino]carbonyl]cyclopentyl]carbonyl]-L-phenylalanine
methyl ester (23-C-1) (C.sub.34H.sub.37Cl.sub.2N.sub.3O.sub.6).
[0389] A solution of 19-A-1 (19-A: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.5 g, 0.91 mmol),
HOBT (0.14 g, 1.04 mmol), EDC (0.19 g, 1.0 mmol), triethylamine
(0.46 mL, 3.28 mmol), and DMAP (0.012 g, 0.1 mmol) are stirred
together in CH.sub.2Cl.sub.2 (dry, 15 mL) in a dry round bottom
flask under a nitrogen atmosphere. Stir for 30 minutes at room
temperature and benzyloxyamine-HCl 23-B (0.26 g, 1.64 mmol) is
added in one portion. The resulting mixture is allowed to stir for
72 hours at room temperature and the solvent is removed in vacuo.
The residue is dissolved in CHCl.sub.3 (50 mL) and the solution is
washed with 1N aq. HCl (50 mL), saturated aq. NaHCO.sub.3 (50 mL),
and the organic layer is dried (MgSO.sub.4). Concentration in vacuo
gives the crude product as a sticky oil which is purified by flash
chromatography on a column of silica gel (5% MeOH, 956
CH.sub.2Cl.sub.2) to give 0.27 g (45%) of 23-C-1 (23-C: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) as a white solid.
[0390] .sup.1H-NMR (300 MHz, CDCl.sub.3): .delta. 8.13 (1H), 7.57
(2H), 7.49 (1H), 7.35 (6H), 7.10 (2H), 5.78 (1H), 4.89 (2H), 3.76
(3H), 3.14 (2H), 2.47 (1H), 2.18 (2H), 1.30-1.90 (4H), 1.27 (3H),
1.15 (3H), 0.80 (3H); Anal. Calcd for
C.sub.34H.sub.37Cl.sub.2N.sub.3O.sub.6.0.24H.sub.2O- : C, 61.97; H,
5.73; N, 6.38; Found: C, 62.02; H, 5.75; N, 6.39; KF Water
0.66%.
Preparation of Example 217
Scheme 23, 23-D: wherein R.sup.4=H, R.sup.5=CO.sub.2H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.23=Phenylmethyl
Stereochemistry=(1S-cis)-L
[0391]
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[2,2,3-trimethyl-3-[[(ph-
enylmethoxy)amino]carbonyl]cyclopentyl]carbonyl-L-phenylalanine
(Example 217) (C.sub.33H.sub.35Cl.sub.2N.sub.3O.sub.6).
[0392] A solution of 23-C-1 (23-C: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3, R.sup.6
4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.075 g, 0.11 mmol) in
methanol (10 mL) is treated with a solution of LiOH--H.sub.2O
(0.024 g, 0.57 mmol) in water (5 mL) over 5 minutes. The mixture is
allowed to stir for 4 hours at room temperature, then the solvent
is removed in vacuo. The crude residue is dissolved in water (10
mL), is filtered through a sintered glass funnel and then the
solution is brought to ca. pH 4 by the addition of 1N aq. HCl. The
resulting solid is isolated by suction filtration, washed with
water (2.times.10 mL), and is then dissolved in acetonitrile-water
(25 mL, 1:3). The solution is frozen and lyophylized to give
Example 217 (23-D: R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl,
R.sup.23=phenylmethyl) (0.048 g, 68%) as a white solid.
[0393] .sup.1H-NMR (300 MHz, MeOH-d.sub.4): .delta. 7.85 (1H), 7.57
(2H), 7.32-7.49 (8H), 7.24 (2H), 4.81 (2H), 4.70 (1H), 3.21 (1H),
2.98 (1H), 2.69 (1H), 2.24 (1H), 1.98 (1H), 1.72 (1H), 1.40 (1H),
1.25 (3H), 1.15 (3H), 0.71 (3H); IR (nujol): 3264, 3195, 3063,
3032, 1731, 1658, 1607, 1502, 1538, 1516, 1432, 1326, 1195, 800
cm.sup.-1; MS (ES+) m/z 640 (M+H.sup.+); Anal. Calcd for
C.sub.33H.sub.35Cl.sub.2N.sub.3O.sub.6-1.19H- .sub.2O: C, 59.87; H,
5.69; N, 6.35; Found: C, 59.70; H, 5.78; N, 6.37; KF Water
3.24%.
Preparation of Example 218
Scheme 23, 23-D: wherein R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.23=H
Stereochemistry (1S-cis)-L
[0394]
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[3-[(hydroxyamino)carbon-
yl]-2,2,3-trimethylcyclopentyl]carbonyl]-L-phenylalanine methyl
ester (Example 218) (C.sub.27H.sub.31Cl.sub.2N.sub.3O.sub.6)
[0395] A solution of 23-C-1 (23-C: R.sup.4=H
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl) (0.7 g, 1.07 mmol) in
THF (120 mL) is hydrogenated over Pd(OH).sub.2 (0.42 g) under 46
psi of hydrogen for 2.75 hours. The catalyst is removed by
filtration through a pad of Celite.RTM., the filter cake is rinsed
with THF (75 mL) and the solvent is removed in vacuo to afford the
crude product as a sticky solid. The crude material is purified by
flash chromatography on a column of silica gel (EtOAc/HOAc,
99.9:0.1) to give Example 218 (23-D: R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3, R.sup.6=4-[(2,6-Dichlorobenzoyl)amin-
o]phenyl, R.sup.23=H) (0.34 g, 56%) as a white solid.
[0396] .sup.1H-NMR (300 MHz, MeOH-d.sub.4): .delta. 7.58 (2H),
7.31-7.49 (3H), 7.22 (2H), 4.72 (1H), 3.69 (3H), 3.18 (1H), 2.99
(1H), 2.73 (1H), 2.29 (1H), 2.02 (1H), 1.75 (1H), 1.42 (1H), 1.27
(3H), 1.18 (3H), 0.75 (3H); IR (nujol): 3313, 3292, 3245, 3194,
3129, 3073, 1749, 1668, 1653, 1606, 1547, 1517, 1459, 1434, 1336,
1211, 1021, 801, 779 cm.sup.-1; MS (FAB) m/z (rel. intensity): 564
(M.sup.++H, 71), 548 (3), 531 (base), 109 (95).
Preparation of Example 219
Scheme 23, 23-D: wherein R.sup.4=H, R.sup.5=CO.sub.2H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]phenyl, R.sup.23=H
Stereochemistry=(1S-cis)-L
[0397]
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[3-[(hydroxyamino)carbon-
yl]-2,2,3-trimethylcyclopentyl]carbonyl]-L-phenylalanine (Example
219) (C.sub.26H.sub.29Cl.sub.2N.sub.3O.sub.6)
[0398] A solution of Example 218 (0.14 g, 0.25 mmol) in methanol (8
mL) is treated with a solution of LiOH--H.sub.2O (0.053 g, 1.27
mmol) in water (4 mL) over 15 minutes. The mixture is allowed to
stir for 1.5 hours at room temperature, then the solvent is removed
in vacuo. The residue is dissolved in water (25 mL), the pH is,
adjusted to ca. 4 with 1N aq. HCl, and the mixture is extracted
with ethyl acetate (3.times.25 mL). The combined organic extracts
are dried (MgSO.sub.4), and concentrated in vacuo to give the crude
material as a sticky solid. The crude product is dissolved in
acetonitrile/water (25 mL, 1:3), and the solution is frozen and
lyophylized to provide Example 219 (23-D: R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl,
R.sup.23=H) (0.098 g, 71%) as a beige solid.
[0399] .sup.1H-NMR (300 MHz, MeOH-d.sub.4): .delta. 7.57 (2H),
7.40-7.47 (3H) 7.24 (2H), 4.71 (1H), 3.20 (1H), 2.99 (1H), 2.69
(1H), 2.29 (1H), 2.03 (1H), 1.75 (1H), 1.43 (1H), 1.28 (3H), 1.18
(3H), 0.76 (3H); IR (nujol): 3262, 3197, 3127, 3070, 1725, 1657,
1607, 1584, 1562, 1535, 1516, 1432, 1326, 1234, 1194, 800, 781
cm.sup.-1; MS (FAB) m/z (rel. intensity): 550 (M.sup.++H, 70), 517
(75), 198 (base); Anal. Calcd for
C.sub.26H.sub.29Cl.sub.2N.sub.3O.sub.6-0.95H.sub.2O: C, 55.02; H,
5.49; N, 7.40; Found: C, 55.29; H, 5.93; N, 7.26; KF Water 3.020.
87
[0400] Boc-Tryptophan-O-methyl ester 24-A (636 mg, 2.00 mmol, 1 eq)
was dissolved in dry DMF. To this solution NaH (88 mg, 2.20 mmol,
1.1 eq) was added with evolution of H.sub.2. To this mixture benzyl
bromide (285 .mu.L, 2.40 mmol, 1.2 eq) was added and the reaction
stirred for 3 hours at room temperature. The reaction was quenched
with brine (15 mL) and extracted with Et.sub.2O (3.times.15 mL).
The combined organics were dried (Na.sub.2SO.sub.4), filtered and
the solvent removed in vacuo. The residue was purified by column
chromatography (SiO.sub.2, Hexanes to 30% EtOAc/Hexanes gradient
elution) to provide 426 mg (52 W) of the benzyl indole 24-B. 88
[0401] The final compound Example 196 was produced as described in
Example 2. ESMS (m/z): 475 (M-H).sup.-. 89
[0402] The preparation of Example 190 is as follows. Boc
Tyr(2,6-dichlorobenzyl)-OH (25-A) (1.31 gm, 2.97 mmol) was
dissolved in THF (5 mL) and cooled to -78.degree. C. under dry
N.sub.2. BH.sub.3 THF (5.9 mL, 5.9 mmol, 1N) was added and the
reaction warmed slowly to room temperature with stirring for 3
hours. The reaction was cooled to 0.degree. C. and quenched with
H.sub.2O (1 mL) and warmed to room temperature. After the addition
of 1 N HCl (25 mL), the mixture was extracted with EtOAc
(3.times.25 ml) and the combined organic phases were dried over
Na.sub.2SO.sub.4. The solution was filtered, solvent evaporated and
the residue chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.50% EtO Ac/hexanes) to provide the intermediate 25-B
(665 mg, 53%): ESMS (m/z) 448 (M+Na).sup.+.
[0403] The above compound, (25-B) (270 mg, 0.634 mmol), was
dissolved dry DMF (5 mL) containing methyl iodide (51 .mu.l, 0.824
mmol). To this solution was added NaH (28 mg, 0.697 mmol: in 60%
oil) and the mixture was stirred for 5 minutes. The reaction was
quenched with the addition of H.sub.2O (1 mL) followed by 1N HCl
(10 ml). The mixture was extracted with EtOAc (3.times.15 mL) and
the combined organics were dried over Na.sub.2SO.sub.4. The
solution was filtered, solvent evaporated and the residue
chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.25% EtOAc/hexanes) to provide intermediate 25-C (130
mg, 47%): ESMS (m/z)462 (M+Na).sup.+.
[0404] 25-C (115 mg, 0261 mmol) was dissolved in 3 N HCl/EtOAc (3
mL) and stirred for 1 h. The solvent was removed under reduced
pressure and dried thoroughly under high vacuum. The residue was
dissolved in THF (5 mL) and DIEA (228 .mu.L, 1.31 mmol) and
(1R)-camphoric anhydride (57 mg, 0.314 mmol) was added. The
reaction was warmed to 60.degree. C. with stirring for 48 h. After
cooling to room temperature, 1N HCl (15 mL) was added and the
mixture extracted with EtOAc (3.times.15 mL). The combined organics
were dried over Na.sub.2SO.sub.4, filtered and the solvent removed
under reduced pressure. The residue was then chromatographed
(SiO.sub.2, gradient elution: 100% hexanes.fwdarw.100% EtOAc) to
provide Example 190 (113 mg. 83%): ESMS (m/z) 422 (MH.sup.+).
90
[0405] Example 56 (0.27 gm, 0.62 mmol) was dissolved in
CH.sub.2Cl.sub.2 (10 mL) and 2,6-dichlorophenylisocyanate (0.18 gm,
0.94 mmol) and DIEA (327 .mu.L, 1.86 mmol) was added and the
reaction stirred overnight. After the addition of 1 N HCl (20 mL),
the mixture was extracted with EtOAc (3.times.25 mL) and the
combined organic phases were dried over Na.sub.2SO.sub.4. The
solution was filtered, solvent evaporated and the residue
chromatographed (SiO.sub.2, gradient elution: 100%
hexanes.fwdarw.33% EtOAc/hexanes) to provide Example 227 (310 mg,
82%): ESMS (m/z) 620 (MH.sup.+).
[0406] Example 227 (250 mg, 0.40 mmol) was dissolved in
CH.sub.2Cl.sub.2 (1.5 mL) and TFA (1.5 mL). After 1 h, the solvent
was removed and the residue triturated with Et.sub.2O (3.times.5
mL) to form a gum. The residue was purified by column
chromatography (SiO.sub.2, gradient elution: 100% hexanes 25%
acetone/hexanes) to provide Example 228 (170 mg, 73%): ESMS (m/z)
564 (MH.sup.+).
[0407] Example 228 (130 mg, 0.23 mmol) was dissolved in
THF/CH.sub.3OH (5 mL/mL, respectively) and LiOH (22 mg, 0.53 mmol)
was added in H.sub.2O (1 mL). After 2 h the solvent was evaporated
and the residue dissolved in H.sub.2O (3 mL). The solution was
precipitated with the addition of 1 N HCl (2 mL). The solvent was
collected by vacuum filtration and washed with cold H.sub.2O
(2.times.2 mL). The solid material was then thoroughly dried under
high vacuum to afford Example 229 (80 mg, 64%) as a white solid:
ESMS (m/z) 550 (MH.sup.+). 91
[0408] Scheme 27 is as follows:
[0409] Example 54 (387 mg, 0.704 mmol) and morpholine (0.14 mL,
1.55 mmol) were dissolved in CH.sub.2Cl.sub.2 (15 mL). This
solution was treated with BOP-Cl (215 mg, 0.845 mmol) and stirred
under dry N.sub.2 at room temperature. After 18 h the reaction was
treated with 1 N HCl (10 mL) and extracted with CH.sub.2Cl.sub.2
(3.times.15 mL). The combined organics were dried over
Na.sub.2SO.sub.4, filtered and the solvent evaporated. The residue
was then purified by column chromatography (SiO.sub.2, gradient
elution: 100% hexanes.fwdarw.100% EtOAc) to provide Example 226
(160 mg, 37%) as a colorless oil: ESMS (m/z) 618 (M+Na).sup.+.
[0410] The methyl ester (Example 226) (160 mg, 0.258 mmol), was
dissolved in THF (5 mL) and LiOH (12 mg, 0.52 mmol) was added in
H.sub.2O (5 mL). After 4 h 1 N HCl (3 mL) was added and the
precipitate collected by vacuum filtration washing with cooled
H.sub.2O (3.times.3 mL) The product was thoroughly dried under high
vacuum to provide Example 225 (148 mg, 95%) as an amorphous powder:
ESMS (m/z) 602 (M-H). 92
[0411] Intermediate used in solid phase synthesis
[0412] Intermediate Urea.
[0413] Camphoric acid methyl ester (10-A) (2.15 g, 11.65 mmol) was
dissolved in THF (25 mL). To this solution was added DPPA
(diphenylphosphoryl azide) (3.33 g, 12.1 mmol) and DIEA (1.73 g,
13.4 mmol). The reaction was warmed at 45.degree. C. with stirring.
After 2.5 h tert-butyl alcohol was added and heated at 85.degree.
C. for an additional 2.5 h. The reaction was worked-up by removing
the volatile components under reduced pressure. The residue was
then purified by flash chromatography (SiO.sub.2, gradient elution:
2% EtOAc/hexanes.fwdarw.20% EtOAc/hexanes) to provide the
symmetrical urea diester (28-A) (2.0 g, 43%): ESMS (m/z) 397
(MH.sup.+).
[0414] The intermediate ester (28-A) (2 g, 5.0 mmol) was dissolved
in THF/CH.sub.3OH (5 mL/2 mL, respectively) and LiOH (490 mg, 11.6
mmol) was added in H.sub.2O (1 mL). After 2 h the solvent was
evaporated, and the residue dissolved in H.sub.2O (5 mL). The
solution was precipitated with the addition of 1 N HCl (1.5 mL).
The solvent was collected by vacuum filtration and washed with cold
H.sub.2O (2.times.2 mL). The solid material was then thoroughly
dried under high vacuum to afford the intermediate symmetrical urea
diacid (28-B) (1.6 g, 89%) as a white solid: ESMS (m/z) 369
(MH.sup.+). 93
[0415] To a solution of
N-(tert-butoxycarbonyl)-4-(2,6-dichlorobenzamido)-- L-phenylalanine
(29-A) (9.25 g, 20.3 mmol) in DMF (100 mL) was added Merrifield
resin (10.0 g, 10.0 meq/g) and anhydrous potassium fluoride (1.57
g, 20.0 mmol). The reaction mixture was stirred for 1 day at
80.degree. C. and the resulting resin bound amino acid was
collected by filtration, washed sequentially with DMF (2.times.200
mL), 50% aqueous DMF (3.times.200 mL), CH.sub.3OH (3.times.300 mL),
CH.sub.2Cl.sub.2 (3.times.300 mL) and CH.sub.3OH (3.times.100 mL)
then dried in vacuo to provide the resin bound
N-(tert-butoxycarbonyl)-4-(2,6-dichlorobenzamido)- -L-phenylalanine
(29-B) (0.53 meq/g). Substitution of the
Boc(L)Phe[4-(2,6-dichlorobenzamido)]-OH onto the resin was
estimated using the picric acid method.
[0416] To the obtained resin (29-B) (150 mg, 0.107 mmol) was added
50% TFA/CH.sub.2Cl.sub.2 (3 mL) and the mixture was shaken for 30
min. The resin was collected by filtration, washed sequentially
with CH.sub.2Cl.sub.2 (2.times.10 mL), CH.sub.3OH (2.times.10 mL),
and CH.sub.2Cl.sub.2 (2.times.10 mL). To the washed resin was added
the symmetrical urea diacid (28-B) (118 mg, 0.320 mmol), 0.5 M DMF
solution of HBTU-HOBT (0.70 mL, 0.320 mmol), DIEA (0.139 mL, 0.799
mmol) and DMF (3.0 mL) and the mixture was vortexed for 2 hrs. at
room temperature. The resin was collected by filtration, washed
sequentially with DMF (2.times.10 mL), CH.sub.2Cl.sub.2 (2 10 mL),
CH.sub.3OH (2.times.10 mL), CH.sub.2Cl.sub.2 (2.times.10 mL). To
the resin bound substrate was added THF (1.6 mL), CH.sub.3OH (0.5
mL) and 2N LiOH (0.310 mL) and the mixture was shaken for 15 mins.
The supernatant was collected by filtration and the resin washed
with THF/5% CH.sub.3OH (2.times.2 mL) and the combined filtrate was
evaporated on a Pierce block evaporator. The concentrate was
diluted with H.sub.2O (1 mL) and the aqueous solution acidified
with 1N HCl (1.5 mL). The precipitate was collected by
centrifugation and the solid washed with H.sub.2O (3.times.3 mL).
The solid material was dried under high vacuum to furnish Example
236 (25 mg, 33%): ESMS (m/z) 701 (M-H).sup.-. 94
Scheme 2, III-a: wherein R.sup.4=H, R.sup.5a=--CH.sub.2CO.sub.2Et,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]-phenyl-Stereochemistry=(S)
[0417] (Intermediate for Examples 201 and 204)
[0418]
(S)-4-[(2,6-Dichlorophenyl)methoxy]-.beta.-[(1,1-Dimethylethoxy)car-
bonyl]amino]benzenebutanoic acid ethyl ester (30-A)
(C.sub.24H.sub.29Cl.sub.2NO.sub.5).
[0419] To a mixture of
Boc-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine (25-A) (5.0 g, 11.36
mmol) and N-methylmorpholine in dry Et.sub.2O at -10.degree. C.
under Ar is added isobutyl chloroformate (1.49 mL, 11.36 mmol). The
reaction is warmed to room temperature, stirred for 1 h, and
filtered. The filtrate is reacted at 0.degree. C. with an excess of
ethereal CH.sub.2N.sub.2. The solution is stirred for 1 h at
0.degree. C., and then is concentrated. The residue is dissolved in
absolute EtOH, and a solution of C.sub.6H.sub.5CO.sub.2Ag (2.86 g,
12.38 mmol) in Et.sub.3N (14 mL) is added slowly. The resulting
mixture is stirred for 1 h at room temperature under Ar and
filtered. The filtrate is concentrated to a dark brown paste. The
product is purified by silica flash chromatography (9:1 and 8:2
hexanes/EtOAc), from which is isolated 2.95 g (6.09 mmol, 54%) of
30-A: TLC R.sub.f=0.32 (7:3 hexanes/EtOAc); [.alpha.].sub.D (C=0.9,
CHCl.sub.3)=-2.degree.; IR (mull) 3360, 2984, 2954, 2925, 2869,
2855, 1721, 1678, 1585, 1524, 1510, 1467, 1447, 1441, 1378, 1373,
1299, 1263, 1251, 1236, 1197, 1177, 1163, 1020, 1016, 783
cm.sup.-1; .sup.1H NMR .delta. 1.27 (3H), 1.41 (9H), 2.38-2.57
(2H), 2.73-2.96 (2H), 4.10-4.20 (3H), 7.22-7.26 (1H), 7.37 (2H); MS
(FAB) m/z 482, 426, 382, 364, 348, 338, 319, 294, 268, 216, 159,
133, 116, 107, 57; Anal. C, 59.67; H, 6.09; Cl, 14.59; N, 3.03
(calcd C, 59.75; H, 6.06; Cl, 14.70; N, 2.90).
[0420]
(S)-4-[(2,6-Dichlorophenyl)methoxy]-.beta.-aminobenzenebutanoic
acid ethyl ester (C.sub.19H.sub.21C.sub.2NO.sub.3). A solution of
the Boc-aminoester (30-A) (0.74 g, 1.53 mmol) in 1:1
CH.sub.2Cl.sub.2/TFA at 0.degree. C. under Ar is stirred for 30 min
at 0.degree. C. and for 1.5 h at room temperature. It is
concentrated, azeotroped thrice wish toluene, and dried to give the
aminoester (30-B) as a solid: TLC R.sub.f=0.15 (EtOAc); .sup.1H NMR
(CHCl.sub.3) .delta. 1.24 (3H), 2.63-2.73 (2H), 2.77-2.92 (1H),
3.07-3.23 (1H), 3.64-3.82 (1H), 4.15 (2H), 5.23 (2H), 6.97 (2H),
7.13 (2H), 7.16-7.25 (1H), 7.36 (2H), 8.16 (2H); MS (FAB) m/z 382,
365, 348, 294, 268, 224, 159, 133, 116, 70. 95
[0421] Scheme 2, III-a: wherein R.sup.4=(CH.sub.3).sub.3CO]C(O)--,
R.sup.5a=-CO.sub.2Me, R.sup.6=4-aminophenyl-, Stereochemistry=L
[0422] (Intermediate for Examples 208, 209, 210)
[0423] N-[(1,1-Dimethylethoxy)carbonyl]-4-nitro-L-phenylalanine
methyl ester (31-B) (C.sub.15H.sub.20N.sub.2O.sub.6) A solution of
N-Boc-4-nitrophenylalanine (31-A) (25.2 g, 81.28 mmol) and DMAP
(0.82 g, 6.7 mmol) in dry DMF is cooled to 0.degree. C. under Ar,
and treated with MeOH (7.55 mL, 186 mmol) and DCC (18.975 g, 91.04
mmol). The reaction mixture is stirred overnight at room
temperature and filtered. The filtrate is washed with satd
NaHCO.sub.3 and brine. The aqueous washes are back-extracted with
CH.sub.2Cl.sub.2. The organics are dried, filtered and concentrated
to a yellow solid. This product is purified by silica flash
chromatography (3:1 hexanes/EtOAc) to give 24.6 g (75.85 mmol, 93%)
of 31-B: TLC R.sub.f=0.36 (7:3 hexanes/EtOAc); .sup.1H NMR
(CHCl.sub.3) .delta. 1.41 (9H), 3.12 (1H), 3.28 (1H), 3.73 (3H),
4.63 (1H), 5.05 (1H), 7.31 (2H), 8.16 (2H); .sup.13C NMR .delta.
28.25, 38.38, 52.56, 54.08, 80.35, 123.67, 130.25, 144.03, 147.12,
154.90, 171.64.
[0424] 4-Amino-N-[(1,1-dimethylethoxy)carbonyl]-L-phenylalanine
methyl ester (31-C) (C.sub.15H.sub.22N.sub.2O.sub.4). A solution of
the above product (2.87 g, 8.85 mmol) in MeOH is treated at room
temperature under N.sub.2 with 100 Pd/C (0.190 g), and hydrogenated
at 40 psi for 3.5 h. The reaction mixture is filtered, and the
filtrate is concentrated to give (31-C) as a dark foam: TLC
R.sub.f=0.34 (1:1 EtOAc/hexane); .sup.1H NMR (CHCl.sub.3) .delta.
1.42 (9H), 2.97 (2H), 3.48 (2H), 3.70 (3H), 4.51 (1H), 4.93 (1H),
5.05 (1H), 6.61 (2H), 6.90 (2H); MS (EI) m/z 294, 238, 221, 207,
193, 177, 161, 135, 118, 106, 91, 77, 57. 96
Scheme 2, III-a: wherein R.sup.4=H.HCl,
R.sup.5a=--CO.sub.2CH.sub.3, R.sup.6=4-benzoylphenyl-,
Stereochemistry=L
[0425] (Intermediate to Examples 40, 191 and 197)
[0426] 4-Benzoyl-L-phenylalanine methyl ester, HCl salt (32-B)
(C.sub.17H.sub.17NO.sub.3.HCl). To cold MeOH (100 mL) under N.sub.2
is added AcCl (10 mL). The solution is stirred at room temperature
for 30 min. 4-Benzoyl-L-phenylalanine (32-A) (0.99 g, 3.7 mmol) is
treated with the methanolic HCl solution (60 mL) at room
temperature for 26 h. The reaction mixture is concentrated to give
1.05 g of the aminoester.HCl salt (32-B) as a solid: TLC (UV)
R.sub.f=0.40 (95:5 CHCl.sub.3MeOH); HPLC t.sub.R=3.0 min (isocratic
650:350:1 CH.sub.3CN/H.sub.2O/TFA); .sup.1H NMR (CD.sub.3OD)
.delta. 7.80-7.70 (4H), 7.68-7.60 (1H), 7.55-7.41 (4H); 4.84 (2H),
4.44 (1H), 3.82 (3H), 3.44-3.27 (2H); .sup.13C NMR (CD.sub.3OD)
.delta. 196.66, 168.86, 139.22, 137.29, 136.91, 132.59, 130.34,
129.62, 129.35, 128.23, 53.56, 52.39, 35.83. 97
[0427] (1S)-1,8,8-Trimethyl-3-oxabicyclo[3.2.1]octane-2,4-dione
[(1S)-camphoric anhydride] (33-A)
(1S-cis)-1,2,2-Trimethylcyclopentane-1,- 3-dicarboxylic acid)
[(1S,3R)-camphoric acid] (1.0 g, 5 mmol) and acetic anhydride (10
mL) were heated at reflux for 3 h. The reaction was cooled and the
solvent was removed on a rotovap (bath temp 60.degree. C.). To the
remainder of the material, saturated NaHCO.sub.3 (2 mL) was added.
The aqueous portion was extracted with CH.sub.2Cl.sub.2 (3.times.5
mL), dried and concentrated in vacuo to 1.08 g. This was triturated
with methyl-t-butyl ether to afford after filtering 0.94 g (103%)
of (1S)-Camphoric anhydride (33-A): mp 222-223.degree. C.;
[.alpha.].sub.D+3.8.degree. (c=0.8, toluene); .sup.1H NMR
(CDCl.sub.3) .delta. 1.01, 1.10, 1.27, 1.89-2.35, 2.84; .sup.13C
NMR (CDCl.sub.3) ppm 172.7, 170.0, 54.33, 53.8, 43.7, 33.5, 24.5,
20.8, 20.2, 14.1; IR (mineral oil mull) 2925, 1804, 1763, 1180,
1128, 1043, 983, 943 cm.sup.-1; MS for C.sub.10H.sub.14O.sub.3, m/z
(relative intensity) 169 (1), 138 (37), 123 (17), 110 (16), 95
(100); Anal. Calcd for C.sub.10H.sub.14O.sub.3: C, 65.92; H, 7.75.
Found: C, 65.86; H, 7.74. 9899
Preparation of Intermediate (34B) (C.sub.5H.sub.3ClINO).
[0428] To a solution of 2-chloro-3-hydroxypyridine (34-A) (10.2 g,
78.7 mmol) and K.sub.2CO.sub.3 (38.87 g, 0.27 mmol) in H.sub.2O
(120 mL) at rt is added 12 (24.33 g, 95.8 mmol). The solution is
stirred at rt for 4 h, and the reaction then is quenched with satd
aqueous Na.sub.2S.sub.2O.sub.5.5H.sub.2O. The pH of the reaction
mixture is adjusted to pH 2 with 12 M aqueous HCl. The mixture is
extracted with EtOAc (3.times.100 mL), and the combined EtOAc
portions dried (MgSO.sub.4), filtered and concentrated to a yellow
solid. Recrystallization of this solid from 120:25 heptane/EtOAc
(145 mL) gives 11.2 g (43.8 mmol, 56%) of intermediate (34-B): IR
3113, 3068, 3056, 3021, 2991, 2955, 2925, 2871, 2855, 2832, 2808,
2749, 2668, 2601, 2530, 1554, 1457, 1398, 1304, 1289, 1226, 1086,
828, 711, 617 cm.sup.-1; .sup.1H NMR (DMSO-d) .delta. 7.06 (1H),
7.59 (1H), 11.06 (1H); .sup.13C NMR (DMSO-d.sub.6) .delta. 101.18,
127.02, 134.98, 138.07, 150.68; Anal. C, 23.32; H, 1.23; Cl, 13.73;
N, 5.42 (calcd C, 23.51; H 1.18, Cl, 13.88; N, 5.48).
[0429] Preparation of Intermediate 34-C
(C.sub.12H.sub.7Cl.sub.3INO).
[0430] To a mixture of (34-B) (5.11 g, 20.0 mmol), Ph.sub.3P (5.30
g, 20.0 mmol) and 2,6-dichlorobenzyl alcohol (3.54 g, 20.0 mmol) in
dry THF (100 mL) at 0.degree. C. is added dropwise DEAD (3.15 mmol,
20.0 mmol). The reaction mixture is stirred an additional 1.5 h at
0.degree. C. and 1.5 h at rt, and then is concentrated. The
reaction product is purified by silica flash chromatography (85:15
hexanes/EtOAc) co give 7.61 g (18.36 mmol, 92%) of (34-C) as a
white solid: TLC R.sub.f=0.57 (7:3 hexanes/EtOAc); .sup.1H NMR
(DMSO-d) .delta. 5.34 (2H), 7.48 (1H), 7.55-7.63 (3H), 7.85 (1H);
MS (ES) m/z 413.8, 327.9, 288.0, 255.9, 183.0, 150.9, 136.9.
[0431] Preparation of Intermediate (34-E)
(C.sub.16H.sub.23NO.sub.7S).
[0432] To a soluition of N-Boc-L-serine methyl ester (34-D) (10.0
g, 45.6 mmol) in anhydrous pyridine (78 mL) at -10.degree. C. under
Ar is added TsCl (10.0 g, 52.4 mmol). The reaction mixture is
stirred for 3 h at -10.degree. C., and then kept at -15.degree. C.
for 66 h. The reaction is quenched with ice, stirred for 2 h, and
then extracted with EtOAc (4.times.300 mL). The combined EtOAc
portions are washed with 0.2 M aqueous KHSO.sub.4 (3.times.300 mL),
H.sub.2O (300 mL), satd aqueous NaHCO.sub.3 (300 mL), and H.sub.2O
(300 mL); and then dried (Na.sub.2SO.sub.4) filtered and
concentrated to a pale yellow-colored oil. The reaction product is
purified by silica flash chromatography (3:1 hexanes/EtOAc) to give
13.0 g (34.8 mmol, 76%) of (34-E) as an off-white colored solid:
TLC Pf=0.25 (7:3 hexanes/EtOAc); IR 3400, 2407, 2313, 2291, 1928,
1741, 1708, 1513, 1350, 1245, 1174, 1159, 1060, 995, 941 cm.sup.-1;
.sup.1H NMR (CDCl.sub.3) .delta. 1.41 (9H), 2.44 (3H), 3.69 (3H),
4.28 (1H), 4.39 (1H), 4.49 (1H), 5.29 (1H), 7.34 (2H), 7.75 (2H);
MS (FAB) m/z 747, 527, 374, 319, 318, 274, 146, 102, 57, 41, 29;
Anal. C, 51.41; H, 6.32; N, 3.87; S, 8.27 (calcd C, 51.46; H, 6.21;
N, 3.75; S, 8.59).
[0433] Preparation of Intermediate (34-F)
(C.sub.9H.sub.16INO.sub.4).
[0434] To a solution of (34-E) (12.82 g, 34.3 mmol) in dry acetone
(40 mL), in an amber reaction flask at rt under Ar, is added
dropwise a solution of NaI (7.73 g, 51.5 mmol) in dry acetone (40
mL). The reaction mixture is stirred at rt for 42 h, and then is
concentrated. The residue is dissolved in CHCl.sub.3 (300 mL). This
CHCl.sub.3 solution is extracted with H.sub.2O (2.times.300 mL),
aqueous 1 M Na.sub.2S.sub.2O.sub.3.5H.sub.2O (300 mL), and H.sub.2O
(3.times.300 mL); and then is dried (Na.sub.2SO.sub.4), filtered,
and concentrated to give a yellow oil. The product is purified by
silica flash chromatography (4:1 hexanes/EtOAc) to give 9.49 g
(28.8 mmol, 84%) of (34-F) as a white solid: TLC R.sub.f=0.52 (7:3
hexanes/EtOAc); .sup.1H NMR (CDCl.sub.3) .delta. 1.45 (9H), 3.56
(2H), 3.79 (3H), 4.51 (1H); MS (FAB) m/z 330, 274, 230, 211, 170,
146, 102, 57, 41.
[0435] Preparation of Intermediate (34-G)
(C.sub.21H.sub.23Cl.sub.3N.sub.2- O.sub.5).
[0436] To a dry amberized reaction flask under Ar, containing
activated Zn dust (0.777 g, 11.89 mmol) and (34-F) (3.91 g, 11.9
mmol), is added dry THF (11.8 mL) and CH.sub.3C(O)N(Me).sub.2 (11.8
mL). Residual O.sub.2 is removed by bubbling Ar through the
suspension for 5 min. The reaction mixture is stirred at
65.+-.5.degree. C. for 2 h, and then is cooled to 0.degree. C. The
PdCl.sub.2[P(Ph).sub.3].sub.2 catalyst (0.41 g) is added, folowed
immediately by an O.sub.2-free solution of (34-C) (2.46 g, 5.94
mmol) in dry 1:1 THF/CH.sub.3C(O)N(Me).sub.2 (17.8 mL). The
resulting reaction mixture is stirred at 65.+-.5.degree. C. under
Ar for 5 h. It is quenched with satd aqueous NH.sub.4Cl (150 mL).
The resulting mixture is extracted with EtOAc (3.times.300 mL). The
combined EtOAc portions are washed with brine (300 mL), dried
(Na.sub.2SO.sub.4), filtered and concentrated to a yellow-green
colored oil. The product is purified by silica flash chromatography
(3:1 hexanes/EtOAc) to give 1.90 g (3.88 mmol, 65%) of (34-G): TLC
R.sub.f=0.32 (7:3 hexanes/EtOAc); IR 3391, 1734, 1702, 1567, 1561;
1508, 1439, 1287, 1256, 1225, 1214, 1198, 1179, 1167, 1152, 1099,
1087, 1070, 1022, 992, 989, 846, 784, 772, 718 cm.sup.-1; .sup.1H
NMR (DMSO-d.sub.6) .delta. 1.31 (9 U), 2.94-3.03 (2H), 3.60 (3H),
4.32 (1H), 5.30 (2H), 7.28 (2H); 7.45-7.58 (3H), 7.76 (1H); MS (ES)
m/z 490.8, 434.8, 388.9.
[0437] Preparation of Intermediate (34-H)
(C.sub.16H.sub.15Cl.sub.3N.sub.2- O.sub.3.HCl).
[0438] A solution of (34-G) (1.90 g, 3.88 mmol) in 4 M HCl in
1,4-dioxane (35 mL) is stirred at rt under Ar for 20 h. The
reaction mixture is concentrated, diluted with H.sub.2O (40 mL),
and extracted with Et.sub.2O (3.times.40 mL). The Et.sub.2O
portions are discarded. The aqueous solution is lyophilized to give
1.39 g (3.26 mmol, 84%) of (34-H) as a beige-colored solid: .sup.1H
NMR (DMSO-d.sub.6) .delta. 3.27 (2H), 3.72 (3H), 4.37 (1H), 5.32
(2H), 7.37 (1H), 7.48 (1H), 7.58 (1H), 7.81 (1H), 8.62 (3H);
.sup.13C NMR (DMSO-d.sub.6) .delta. 36.29, 51.98, 53.14, 66.74,
123.33, 125.04, 129.38, 131.21, 132.50, 136.63, 138.99, 147.02,
149.88, 169.75.
[0439] Preparation of Intermediate (34-I)
(C.sub.30H.sub.37Cl.sub.3N.sub.2- O.sub.6).
[0440] To a reaction mixture containing acid (15D) (0.513 g, 2.0
mmol), EDC (0.403 g, 2.06 mmol), HOBt (0.284 g, 2.10 mmol), DMAP
(0.076 g, 0.62 mmol) and (34-H) (0.878 g, 2.06 mmol) in
CH.sub.2Cl.sub.2 (20.4 mL) at 0.degree. C. is added Et.sub.3N (1.02
mL, 7.24 mmol). The mixture is stirred for 2 h at 0.degree. C. and
44 h at rt. It is diluted with CH.sub.2Cl.sub.2 (200 mL). The
CH.sub.2Cl.sub.2 mixture is washed with H.sub.2O (3.times.200 mL),
0.5 M aqueous HCl (2.times.200 mL), satd aqueous NaHCO.sub.3
(2.times.200 mL), and H.sub.2O (2.times.200 mL). The combined
aqueous washes are extracted with one portion of CH.sub.2Cl.sub.2
(200 mL). The combined CH.sub.2Cl.sub.2 portions are dried
(Na.sub.2SO.sub.4), filtered, and concnetrated to give a
yellow-colored oil. The product is purified by silica flash
chromatography (3:2 hexanes/EtOAc) to give 0.919 g (1.46 mmol, 73%)
of (34-I): TLC R.sub.f=0.15 (7:3 hexanes/EtOAc); .sup.1H NMR
(CDCl.sub.3) .delta. 0.82 (3H), 1.18 (3H), 1.31 (3H), 1.38-1.48
(1H), 1.44 (9H), 1.58-1.81 (1H), 2.14-2.27 (1H), 2.49-2.70 (2H),
3.17 (1H), 3.29 (1H), 3.69 (3H), 4.93 (1H), 5.33 (2H), 7.06 (2H),
7.26-7.39 (4H); .sup.13C NMR (CDCl.sub.3) .delta. 14.19, 20.40,
21.93, 22.19, 22.91, 28.07, 32.36, 37.04, 46.41, 51.76, 52.28,
54.50, 56.78, 60.38, 61.17, 66.73, 80.07, 123.08, 123.23, 128.60,
130.92, 130.96, 137.08, 140.97, 149.43, 149.77, 171.71, 172.70,
175.11.
[0441] Preparation of Intermediate (34-J)
(C.sub.26H.sub.29Cl.sub.3N.sub.2- O.sub.6).
[0442] To the solid (34-I) (0.910 g, 1.45 mmol) at 0.degree. C.
under Ar is added slowly TFA (9 mL). The resulting solution is
stirred for 30 min at 0.degree. C. and 2 h at rt. The reaction
mixture is concentrated in vacuo, thrice azeotroped with
PhCH.sub.3, and dried under vacuum to give (34-J) as an
amber-colored foam: TLC R.sub.f=0.37 (750;250:5
hexanes/EtOAc/HCO.sub.2H); IR 3321, 3061, 1746, 1728, 1696, 1655,
1584, 1565, 1523, 1497, 1440, 1355, 1286, 1209, 1200, 1171, 1119,
1094, 1088, 995, 781, 769, 731, 717, 695 cm.sup.-1; .sup.1H NMR
(CDCl.sub.3) .delta. 0.87 (3H) 1.26 (3H), 1.33 (3H), 1.46-1.58
(1H), 1.73-1.87 (1H), 2.14-2.30 (1H), 2.53-2.65 (2H), 2.69 (1H),
3.19 (1H), 3.29 (1H), 3.69 (3H), 4.93 (1H), 5.33 (2H), 7.07 (1H),
7.23-7.40 (4H), 10.18 (1H).
[0443] Preparation of Example 194
(C.sub.26H.sub.29Cl.sub.3N.sub.2O.sub.6)- .
[0444] To a solution of (34-J) (0.908 g, 1.41 mmol) in THF (28.9
mL) is added a solution of LiOH.H.sub.2O (0.291 g, 6.94 mmol) in
H.sub.2O (14.4 mL). The reaction mixture is stirred for 5 h, and
then is diluted with H.sub.2O (70 mL). It is cooled to 0.degree.
C., acidified with 1 M aqueous HCl, and extracted with EtOAc
(3.times.200 mL). The combined EtOAc portions are washed with brine
(200 mL), dried (Na.sub.2SO.sub.4), filtered and concentrated to a
viscous colorless oil. The product is purified by silica flash
chromatography (200:50:1 hexanes/EtOAc/HCO.sub.2- H), azeotroped
thrice from PhCH.sub.3, dissolved in 1:1 CH.sub.3CN/H.sub.2O (40
mL), and lyophilized to give 0.752 g (1.35 mmol, 96%) of Example
194 as a white solid: mp 120-122.degree. C.; TLC R.sub.f=0.28
(200:1 EtOAc/HCO.sub.2H); IR 3323, 3064, 2730, 2668, 1714, 1700,
1648, 1584, 1564, 1522, 1440, 1354, 1284, 1235, 1198, 1162, 1118,
1096, 1089, 995, 862, 828, 780, 769, 716 cm.sup.-1; .sup.1H NMR
(DMSO-d.sub.6) .delta. 0.63 (3H) 1.09 (3H), 1.12 (3H), 1.24-1.37
(1H), 1.46-1.58 (1H), 1.78-1.94 (1H), 2.27-2.43 (2H), 2.62 (1H),
2.96-3.14 (2H), 3.31 (1H), 4.57 (1H), 5.30 (2H), 7.29 (1H),
7.45-7.58 (3H), 7.75 (1H), 7.85 (1H) MS (EI) m/z 556, 538, 379,
353, 335, 301, 159, 142, 123, 109, 95. 100101
Preparation 35-B: Scheme 35
[0445] To a cooled (0-5.degree. C.) mixture of Wang polystyrene
resin 35-A (Advanced Chemtech, 2.0 g, ca. 1.5 mmol),
N-Boc-4-iodo-L-phenylalanine (4.00 g, 10 mmol), and PPh.sub.3 (1.30
g, 5.0 mmol) in THF (20 mL) was added diethyl azodicarboxylate
(0.80 mL, 5.0 mmol) in 4 approximately equal portions at 5 min
intervals. When the orange color had discharged the mixture was
warmed to ambient temperature and stirred for 5 h. The mixture was
diluted with THF (30 mL) and filtered. The resin was washed with
DMF (5.times.50 mL), THF (5.times.50 mL), and MeOH (3.times.50 mL)
and then dried in vacuo to afford the esterified resin 35-B (2.68
g) as a colorless powder: .sup.13C NMR (100 MHz, CD.sub.2Cl.sub.2,
4 mm MAS probe) .delta. 171.86, 155.33, 137.85, 136.40, 131.87,
128.00, 92.74, 80.09, 54.05, 38.05, 28.51.
[0446] Preparation 35-C-1: Scheme 35 where R.sup.35 is
2,4,6-trichlorophenyl.
[0447] N.sub.2 was bubbled through a mixture of
N-Boc-4-iodo-L-phenylalani- ne functionalized Wang resin (35-B)
(500 mg, ca. 0.3 mmol), PPh.sub.3 (105 mg, 0.4 mmol),
2,4,6-trichloroaniline (490 mg, 2.5 mmol) and DIEA (1.74 mL, 10
mmol) in NMP (10 mL) for 10 min. Pd.sub.2 dba.sub.3 (92 mg, 0.1
mmol) was added and the reaction mixture was placed under a CO
atmosphere and heated (bath temp. 70.degree. C.) for 18 h. Upon
cooling to ambient temperature the mixture was diluted with 3%
(w/v) sodium diethyldithiocarbamate in 95:5 NMP:DIEA (10 mL). After
an additional 10 min the mixture was filtered and the resin washed
with NMP (5.times.10 mL), THF (3.times.10 mL), and MeOH (3.times.10
mL) and dried in vacuo to afford 35-C-1 as a colorless powder.
[0448] Preparation 35-D-1: Scheme 35 where R.sup.35 is
2,4,6-trichlorophenyl.
[0449] Resin 35-C-1 was swollen with methylene chloride (0.5 mL)
and diluted with 95:5 TFA:H.sub.2O (10 mL) After 90 min the mixture
was filtered and the resin washed with TFA (3.times.5 mL),
CH.sub.2Cl.sub.2 (3.times.5 mL) and MeOH (3.times.5 mL). The
combined filtrates were concentrated in vacuo and the residue
lyophilized from glacial acetic acid to provide the amino acid
35-D-1 (152 mg, 91%) as a powder which was used without
purification: MS (FAB) m/z (rel. intensity) 387 (M+H, 42), 427
(26), 426 (80), 389 (46), 387 (42), 366 (33), 279 (99), 177 (54),
146 (18), 119 (26), 23 (26); HRMS (FAB) calcd for
C.sub.16H.sub.13Cl.sub.3N.s- ub.2O.sub.3+H.sub.1 387.0070, found
387.0084.
[0450] Preparation 35-E-1: Scheme 35 where R.sup.35 is
2,4,6-trichlorophenyl.
[0451] The amino acid 35-D-1 was dissolved in methanolic HCl (20
mL) and heated at 55.degree. C. for 18 h. Concentration in vacuo
afforded the methyl ester 35-E-1 which was used without
purification: MS (ES-+) for C.sub.17H.sub.15Cl.sub.3N.sub.2O.sub.3
m/z 400.9 (M+H).sup.+.
[0452] Preparation 35-F-1: Scheme 35 where R.sup.35 is
2,4,6-trichlorophenyl.
[0453] To a cooled (0-5.degree. C.) solution of the methyl ester
35-E-1,15-D (97 mg, 0.38 mmol), and 1-hydroxy-7-azabenzotriazole
(52 mg, 0.38 mmol) in CH.sub.2Cl.sub.2/DMF (1:2, 6 mL) was added
EDC (73 mg, 0.38 mmol) followed by DIEA (0.23 ml, 1.14 mmol). The
solution was gradually allowed to warm to ambient temperature and
stirred an additional 16 h. Volatiles were removed in vacuo and the
residue partioned between ethyl acetate and 0.25N aq. HCL. The
organic layer was washed with saturated aq. NaHCO.sub.3 and brine,
dried (MgSO.sub.4), filtered and concentrated in vacuo.
Purification of the residue by flash chromatography using ethyl
acetate/CH.sub.2Cl.sub.2/hexane (1:1:6) as eluant afforded 35-F-1
(115 mg) as an amorphous powder: .sup.1H NMR (300 MHz, CDCl.sub.3)
.delta. 7.96 (1H), 7.86 (2H), 7.20 (2H), 5.86 (1H), 4.92 (1H), 3.72
(3H), 3.17 (2H), 2.49 (2H), 2.10 (1H), 1.69 (1H), 1.41 (10H), 1.25
(3H), 1.19 (3H), 0.76 (3H); .sup.13C NMR (75 MHz, CDCl.sub.3)
.delta. 174.90, 172.66, 171.88, 165.41, 140.88, 134.42, 133.50,
132.08, 131.27, 129.58, 128.44, 127.97, 80.25, 60.39, 56.64, 54.43,
52.91, 52.47, 46.38, 37.71, 32.33, 28.03, 22.99, 22.51, 21.93,
20.59, 14.17; MS (FAB) m/z (rel. intensity) 639 (M+H, 17), 641
(17), 639 (17), 583 (16), 403 (27), 401 (28), 189 (23), 137 (18),
109 (99), 57 (59), 41 (20); HRMS (FAB) calcd for
C.sub.31H.sub.37Cl.sub.3N.sub.2O.sub.6+H, 639.1795, found
639.1779.
Preparation of Example 220
[0454]
(1S-cis)-N-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]-carbonyl]-4-[[[-
(1,1'-biphenyl)-4-yl)amino]-carbonyl]-L-phenylalanine
[0455] Example 220 was prepared as described in Schemes 35 and 2
starting from 4-aminobiphenyl and 35-B: physical properties as
follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.87 (1H), 7.76
(2H), 7.62 (4H), 7.44 (4H), 7.30 (1H), 4.79 (1H), 3.30 (1H), 3.01
(1H), 2.72 (1H), 2.54 (1H), 2.02 (1H), 1.62 (1H), 1.58 (1H), 1.28
(3H), 1.20 (3H), 0.78 (3H); .sup.13C NMR (75 MHz, CD.sub.3OD)
.delta. 179.75, 175.49, 174.98, 168.82, 143.38, 142.03, 139.42,
138.75, 134.74, 130.70, 130.01, 128.86, 128.38, 128.31, 127.87,
122.68, 57.56, 54.69, 47.81, 38.43, 33.87, 23.94, 23.25, 22.57,
21.99; MS (FAB) m/z (rel. intensity) 543 (M.sup.+, 17), 109 (41),
83 (43), 81 (37), 71 (45), 69 (82), 67 (35), 57 (81), 55 (99), 43
(80); HRMS (EI) calcd for C.sub.32H.sub.34N.sub.2O.sub.6 542.2416,
found 542.2429. Anal. Calcd for
C.sub.32H.sub.34N.sub.2O.sub.6.0.5H.sub.2O: C, 69.67; H, 6.39; N,
5.08. Found: C, 69.72; H, 6.65; N, 4.75.
Preparation of Example 221
[0456]
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(4-c-
hlorophenyl)amino]-carbonyl]-L-phenylalanine
[0457] Example 221 was prepared as described in Schemes 35 and 2
starting from 4-chloroaniline and 35-B: physical properties as
follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.85 (2H), 7.68
(2H), 7.40 (2H), 7.36 (2H), 4.82 (1H), 3.29 (1H), 3.05 (1H), 2.81
(1H), 2.54 (1H), 1.96 (1H), 1.70 (1H), 1,24 (1H), 0.91 (3H), 0.86
(3H), 0.77 (3H); .sup.13C NMR (75 MHz, CD.sub.3OD) .delta. 179.74,
175.55, 174.65, 168.73, 143.39, 138.93, 134.-5, 130.66, 130.55,
128.86, 123.68, 57.54, 54.62, 47.81, 38.33, 35.92, 33.86, 30.30,
29.00, 26.34, 23.21, 22.51, 21.98, 21.16; HRMS (FAB) calcd for
C.sub.26H.sub.29ClN.sub.2O.sub.6+H.sub.1 501.1792, found
501.1790.
Preparation of Example 222
[0458]
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]-carbonyl]-4-[[(2--
trifluoromethylphenyl)amino]-carbonyl]-L-phenylalanine
[0459] Example 222 was prepared as described in Schemes 35 and 2
starting from 2-(triflouromethyl)aniline and 35-B. Physical
properties as follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta.
7.86 (2H), 7.66 (4H), 7.40 (2H), 4.78 (1H), 3.30 (1H), 3.10 (1H),
2.72 (1H), 2.49 (1H), 1.98 (1H), 1.62 (1H), 1.48 (1H), 1.28 (3H),
1.20 (3H), 0.78 (3H); .sup.13C NMR (75 MHz, CD.sub.3OD) .delta.
179.70, 175.77, 173.54, 169.59, 143.34, 136.74, 124.18, 133.85,
131.74, 130.73, 128.89, 128.30, 127.13, 123.51, 57.56, 55.05,
62.54, 57.56, 55.05, 54.58, 50.06, 47.80, 38.21, 33.84, 23.89,
23.19, 22.52, 22.30, 21.85; IR (mull) 3302, 1708, 1656, 1613, 1592,
1530, 1508, 1320, 1294, 1260, 1206, 1173, 1123, 1059, 767
cm.sup.-1; MS (FAB) m/z (rel. intensity) 535 (M.sup.+, 99), 536
(32), 535 (99), 517 (25), 353 (46), 109 (57), 69 (14), 57 (13), 55
(14), 43 (13); HRMS (FAB) m/z calcd for
C.sub.27H.sub.29F.sub.3N.sub.2O.sub.6+H.sub.1 535.2056, found
535.2049. Anal. Calcd for
C.sub.27H.sub.29F.sub.3N.sub.2O.0.5H.sub.2O: C, 59.66; H, 5.56; N,
5.15. Found: C, 59.75; H, 5.73; N, 4.72.
Preparation of Example 223
[0460]
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(2,4-
,6-trichlorophenyl)amino]carbonyl]-L-phenylalanine
[0461] 35-F-1 (110 mg, 0.16 mmol) was deprotected as described in
Scheme 2 to afford example 223 (90 mg) as an amorphous powder:
.sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.90 (2H), 7.60 (1H),
7.41 (2H), 4.82 (1H), 3.29 (1H), 3.10 (1H), 2.72 (1H), 2.63 (1H),
1.92 (1H), 1.68 (1H), 1.44 (1H), 0.88 (6H), 0.77 (3H); .sup.13C NMR
(75 MHz, CD.sub.3OD) .delta. 179.72, 175.52, 174.72, 168.90,
143.97, 136.86, 135.15, 133.59, 133.02, 130,82, 129.66, 129.09,
62.70, 57.53, 54.65, 38.41, 33.85, 23.91, 23.20, 22.53, 21.97; IR
(mull) 3263, 3079, 1709, 1657, 1614, 1573, 1556, 1524, 1495, 1287,
1246, 1205, 1190, 869, 857 cm.sup.-1; MS (ES+) for
C.sub.26H.sub.27Cl.sub.3N.sub.2O.sub.6 m/z 568.9 (M+H).sup.+; Anal.
Calcd for O.sub.26H.sub.27Cl.sub.3N.sub.2O.sub.6: C, 54.80; H,
4.78; N, 4.92. Found: C, 55.00; H, 5.08; N, 4.64.
Preparation of Example 224
[0462]
[1S-[1.alpha.(R*),3.alpha.]]-4-[[[(1-Carboxy-3-methylbutyl]-amino]c-
arbonyl]-N-[[(3-carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-L-phenylalani-
ne
[0463] Example 224 was prepared as described in Schemes 35 and 2
starting from methyl L-leucinate and 35-B: Physical properties as
follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 7.78 (2H), 7.34
(2H), 4.87 (1H), 4.72 (1H), 3.30 (1H), 3.02 (1H), 2.82 (1H), 2.68
(1H), 1.98 (1H), 1.74 (5H), 1.23 (3H), 0.97 (9H), 0.77 (3H);
.sup.13C NMR (75 MHz, CD.sub.3OD) .delta. 179.73, 176.33, 175.23,
174.66, 170.35, 143.05, 133.87, 130.52, 128.72, 62.41, 57.53,
54.63, 52.77, 47.79, 41.51, 38.30, 26.42, 23.90, 23.56, 23.18,
22.51, 21.93; MS (FAB) m/z (rel. intensity) 505 (M.sup.+, 99), 506
(27), 505 (99), 487 (20), 109 (29), 71 (20), 69 (34), 57 (34), 55
(33), 43 (36); HRMS (FAB) calcd for
C.sub.26H.sub.36N.sub.2O.sub.8H.su- b.1 505.2549, found 505.2570.
102
[0464] Where R.sup.36-1 and R.sup.36-2 are independently defined as
--CH.sub.3, t-Bu, or --CH.sub.2C.sub.6H.sub.5 and relative
configuration is depicted by bold and dotted lines.
[0465] Scheme 36 teaches a general method (etherification,
epimerization and ester deprotection) for the preparation of
selectively protected camphoric acid isomers 36-B, 36-D, 36-G and
36-F. 103
[0466] Where R.sup.37-1 and R.sup.37-2 are independently defined as
--H or --CH.sub.3 and R.sup.37-3 and R.sup.37-4 are independently
defined as --H, --CH.sub.3, t-Bu, or --CH.sub.2C.sub.6H.sub.5.
[0467] Scheme 37 teaches methods for the coupling of camphoric acid
monoester isomers to isomers of 37-B and ester deprotection for the
preparation of isomers of Example 54. 37-C-1 through 37-C-15.
Preparation of Example 237
Scheme 37: 37-C-1 where R.sup.3 is H, R.sup.1 is CH.sub.3, and the
stereochemistry is (1S-cis) and D-Phenylalanine
[0468]
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-
-dichlorobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.- sub.6), 37-C-1 was prepared as
follows:
[0469] To a solution of
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxy- lic acid
1-(1,1-dimethylethyl)-3-methyl diester (502.7 mg), 15-C, in
methylene chloride (8 mL) at 0.degree. C. was added DIEA (1 mL),
EDC (413.1 mg), HOBT (291.1 mg), and dimethylaminopyridine (26.4
mg). The reaction was stirred at 0.degree. C. for 15 minutes and
then 4-[(2,6-dichlorobenzoyl)amino]-D-phenylalanine methyl ester,
hydrochloride salt (803 mg), 37-B-2, was added and stirred at
ambient temperature for 50 hours. The reaction was diluted with
water and extracted with methylene chloride. The extracts
concentrated in vacuo and the crude material purified by flash
chromatography over silica gel. The crude material was applied to
the column by concentrating it on a plug of silica gel and adding
this plug to the top of the column. The column was eluted with
methanol in methylene chloride to obtain
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]-
carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenylalanine methyl
ester (890 mg). Physical properties as follows: m.p.
265-270.degree. C.; .sup.1H NMR (CDCl.sub.3) .delta. 7.63 (2H),
7.35 (3H), 7.19 (2H), 4.84 (1H), 3.76 (3H), 3.18 (1H), 3.01 (1H),
2.61 (1H), 2.47 (1H), 2.06 (1H), 1.74 (1H), 1.44 (10H), 1.14 (3H),
1.03 (3H), 0.66 (3H); MS-ESI (m/z): 603 ([M-H.sup.-]); MS-ESI
(m/z): 605 ([M+H.sup.+]). The deprotection of the carboxylic acids
follows that of Examples 53, and 54 to obtain
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,
6-dichlorobenzoyl)amino]-D-phenylalanine. Physical properties as
follows: m.p. 263-267.degree. C.; .sup.1H NMR (300 MHz,
DMSO-d.sub.6). .delta. 10.6 (1H), 7.83 (1H), 7.52 (5H), 7.2 (2H),
4.45 (1H), 3.1 (1H), 2.82 (1H), 2.62 (1H), 2.3 (1H), 1.94 (1H),
1.55 (1H), 1.3 (1H), 1.05 (3H), 0.83 (3H), 0.45 (3H); MS-ESI (m/z):
533 ([M-H.sup.-); MS-ESI (m/z): 535 ([M+H.sup.+]).
Preparation of Example 238
[0470] Scheme 37: 37-C-2 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1S-trans) and D-Phenylalanine
(1S-trans)-N-[(3-Carbox-
y-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-2 was
prepared as follows:
[0471] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1S-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-G-1, and
4-[(2,6-dichlorobenzoyl)ami- no]-D-phenylalanine methyl ester,
hydrochloride salt, 37-B-2. Physical properties as follows: m.p.
158-168.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6). .delta.
12.3 (1H), 10.5 (1H), 7.95 (1H), 7.5 (5H), 7.19 (2H), 4.43 (1H),
3.04 (1H), 2.80 (1H), 2.66 (1H), 1.97 (2H), 1.66 (1H), 1.42 (1H),
0.96 (3H), 0.70 (3H), 0.44 (3H); MS-ESI-- (m/z): 533 ([M-H.sup.-]);
MS-ESI (m/z): 535 ([M+H.sup.+]).
Preparation of Example 239
[0472] Scheme 37: 37-C-3 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1S-trans) and L-Phenylalanine
(1S-trans)-N-[(3-Carbox-
y-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-3 was
prepared as follows:
[0473] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1S-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-G-1, and
4-[(2,6-dichlorobenzoyl)ami- no]-L-phenylalanine methyl ester,
hydrochloride salt, 37-B-1. Physical properties as follows: m.p.
172-178.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
10.6 (1H), 7.93 (1H), 7.5 (5H), 7.19 (2H), 4.43 (1H), 2.98 (1H),
2.86 (1H), 2.68 (1H), 2.01 (2H), 1.89 (1H), 1.63 (1H), 1.43 (1H),
1.02 (3H), 0.99 (3H), 0.72 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]
Preparation of Example 240
[0474] Scheme 37: 37-C-4 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1R-trans) and L-Phenylalanine
(1R-trans)-N-[(3-Carbox-
y-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-4 was
prepared as follows:
[0475] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1R-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-G-2, and
4-[(2,6-dichlorobenzoyl)ami- no]-L-phenylalanine methyl ester,
hydrochloride salt, 37-B-1. Physical properties as follows: m.p.
168-170.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
12.3 (2H), 10.6 (1H), 7.96 (1H), 7.51 (5H), 7.20 (2H), 4.43 (1H),
3.05 (1H), 2.80 (1H), 1.97 (2H), 1.66 (1H), 1.42 (1H), 0.96 (3H),
0.70 (3H), 0.44 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI (m/z)
535 ([M+H.sup.+]).
Preparation of Example 241
[0476] Scheme 37: 37-C-5 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1R-trans) and D-Phenylalanine
(1R-trans)-N-[(3-Carbox-
y-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6) 37-C-5 was
prepared as follows:
[0477] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1R-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-G-2, and
4-[(2,6-dichlorobenzoyl)ami- no]-D-phenylalanine methyl ester,
hydrochloride salt, 37-B-2. Physical properties as follows: m.p.
158-165.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6). 10.6 (1H),
7.93 (1H), 7.51 (5H), 7.19 (2H), 4.43 (1H) 2.98 (1H), 2.86 (1H)
2.68 (1H), 2.02 (1H), 1.88 (1H), 1.62 (1H)J, 1.43 (1H), 1.02 (3H),
0.991 (3H), 0.73 (3H); MS-ESI (m/z): 533 ([M-H]); MS-ESI (m/z):
5351 (M+H.sup.+])
Preparation of Example 242
[0478] Scheme 37: 37-C-6 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1R-cis) and D-Phenyalaanine
(1R-cis)-N-[(3-Carboxy-2,-
2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenyl-
alanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-6 was
prepared as follows:
[0479] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-D, and
4-[(2,6-dichlorobenzoyl)amino]-D-p- henylalanine methyl ester,
hydrochloride salt, 37-B-2. Physical properties as follows: m.p.
166-170.degree. C.; .sup.1H NMR (300 MHz, DMSO-d,) .delta. 10.65
(1H), 7.81 (1H), 7.51 (5H), 7.20 (2H), 4.44 (1H), 2.99 (1H), 2.85
(1H), 2.68 (11H), 2.34 (1H), 1.88 (1H), 1.52 (1H), 1.30 (1H), 1.15
(3H), 1.10 (3H), 0.65 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI
(m/z): 535 ([M+H.sup.+]).
Preparation of Example 243
[0480] Scheme 37: 37-C-7 where R.sup.3 is H, R.sup.1 is CH.sub.3,
and the stereochemistry is (1R-cis) and L-Phenylalanine
(1R-cis)-N-[(3-Carboxy-2,-
2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenyl-
alanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-7 was
prepared as follows:
[0481] The preparation follows that of Preparation 37-C-1. The
starting materials are
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-ester, 36-D, and
4-[(2,6-dichlorobenzoyl)amino]-L-p- henylalanine methyl ester,
hydrochloride salt, 37-B-1. Physical properties as follows: m.p.
171-172.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
10.6 (1H), 7.82 (1H), 7.51 (5H), 7.18 (2H), 4.45 (1H), 3.1 (1H),
2.82 (1H), 2.62 (1H), 2.32 (1H), 1.93 (1H), 1.58 (1H), 1.34 (1H),
1.06 (3H), 0.83 (3H), 0.45 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]);
MS-ESI (m/z) 535 ([M+H.sup.+]).
Preparation of Example 244
[0482] Scheme 37: 37-C-8 where R.sup.3 is CH.sub.3, R.sup.3 is H,
and the stereochemistry is (1S-cis) and L-Phenylalanine
(1S-cis)-N-[(3-Carboxy-1,-
2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenyl-
alanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-8 was
prepared as follows:
[0483] To a solution of
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxy- lic acid
3-methyl ester, 36-B (501.3 mg) in DMF (5 mL) and DIEA (3 mL) was
added O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium
hexafluorophosphate (1.0445 g) and the reaction stirred at ambient
temperature for 1 hour. To the reaction was added
4-[(2,6-dichlorobenzoyl- )amino]-L-phenylalanine methyl ester,
hydrochloride salt (1.0093 g), 37-B-1, and the reaction stirred for
3 days. The reaction was then diluted with water and extracted with
AcOEt. The concentrated extract was purified by flash
chromatography on silica gel eluting with methanol in methylene
chloride to obtain (1S-cls)-N-[(3-Methoxycarbonyl)-1,2,2-trimet-
hylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (2.272 g). A solution of LiOH (552 mg) in H.sub.2O (20
mL) and 4 mL of 30% hydrogen peroxide was added to a solution of
the above
(1S-cis)-N-[((3-Methoxycarbonyl)-1,2,2-trimethylcyclopentyl)carbonyl]-4-[-
(2,6-dichlorobenzoyl)amino]-L-phenylalanine methyl ester
(C.sub.28H.sub.32Cl.sub.2N.sub.2O.sub.6) (2.272 g) in 15 mL of
methanol. The solution was stirred for 6 days. The methanol is then
removed in vacuo. The aqueous layer is further diluted with water
and extracted with diethyl ether and the extract discarded. The
aqueous layer is acidified to pH=3-4 with 0.6N HCl resulting in a
precipitate. The precipitate is filtered washing with water to
obtain (1S-cis)-N-[(3-Carboxy-1,2,2-trimet-
hylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(1.1265 g. Physical properties as follows: m.p. 152-157.degree. C.
.sup.1H NMR (300 MHz, DMSO-d.sub.6). .delta. 12.3 (1H), 10.63 (1H),
7.50 (5H) 7.27 (1H), 7.18 (2H), 4.45 (1H), 2.99 (2H), 2.63 (1H),
2.25 (1H), 1.93 (1H), 1.69 (1H), 1.28 (1H), 1.16 (3H), 1.05 (3H),
0.50 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI (m/z): 535
([M+H.sup.+]).
Preparation of Example 245
[0484] Scheme 37: 37-C-9 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1S-cis) and D-Phenylalanine
(1S-cis)-N-[(3-Carboxy-1,-
2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenyl-
alanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6) 37-C-9 was
prepared as follows:
[0485] The preparation follows that of Preparation 37-C-8. The
starting materials are
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid 3-methyl
ester, 36-B, and 4-[(2,6-dichlorobenzoyl)amino]-D-phenylalanine
methyl ester, hydrochloride salt, 37-B-2. Physical properties as
follows: m.p. 155-163.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.)
.delta. 12.3 (1H), 10.6 (1H), 7.51 (5H), 7.29 (1H), 7.18 (2H), 4.44
(1H), 3.07 (1H), 2.94 (1H), 2.25 (1H), 2.62 (1H), 1.94 (1H), 1.67
(1H), 1.28 (1H), 1.04 (3H), 0.99 (3H), 0.48 (3H); MS-ESI (m/z): 533
([M-H.sup.-]); MS-ESI (m/z): 535 ([M+H.sup.+]).
Preparation of Example 246
[0486] Scheme 37: 37-C-10 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1S-trans) and D-Phenylalanine
(1S-trans)-N-[(3-Carbox-
y-1,2,2-trimethylcyclopentyl)carbonyl]-4-[[(2,6-dichlorobenzoyl)amino]-D-p-
henylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6) 37-C-10 was
prepared as follows:
[0487] To a solution of
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trime-
thylcyclopentane-1-carboxylic acid, 36-F-1, (503.7 mg) in DMF (5
mL) and DIEA (3 mL) was added
O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluro- nium
hexafluorophosphate (731.7 mg) and the reaction stirred at ambient
temperature for 1 hour. To the reaction was added
4-[(2,6-dichlorobenzoyl- )amino]-D-phenylalanine methyl ester,
hydrochloride salt (698.6 mg), 37-B-2, and the reaction stirred for
5 days. The reaction was then diluted with water and extracted with
AcOEt. The concentrated extract was purified by flash
chromatography on silica gel eluting with methanol in methylene
chloride to obtain (1S-trans)-N-[(3-(Phenylmethoxy)carbonyl)-1,-
2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenyl-
alanine methyl ester (1.1024 g). Physical properties as follows:
.sup.1H NMR (CDCl.sub.3) .delta. 7.82 (1H), 7.58 (2H), 7.28 (8H),
7.05 (2H), 6.07 (1H), 5.06 (2H), 4.74 (1H), 3.66 (3H), 3.07 (3H),
2.05 (3H), 1.53 (1H), 1.21 (3H), 0.90 (3H), 0.75 (3H); MS-ESI
(m/z): 637 ([M-H.sup.-]); MS-ESI (m/z): 661 ([M+Na].sup.+).
[0488] The product from above was dissolved in THF (10 mL) and 10%
palladium on carbon (75 mg) was added and the mixture hydrogenated
at atmospheric pressure for 26 hours. The reaction was then
filtered and the filtrate concentrated to obtain
(1S-trans)-N-1(3-Carboxy-1,2,2-trimethylc-
yclopentyl)carbonyl]-4-[(2,
6-dichlorobenzoyl)amino]-D-phenylalanine methyl ester (896.3 mg).
Physical properties as follows; MS-ESI (m/z): 547 ([M-H.sup.-]);
MS-ESI (m/z): 571 ([M+Na].sup.+).
[0489] A solution of LiOH (213.2 mg) in H.sub.2O (10 mL) and 2 mL
of 30% hydrogen peroxide was added to a solution of
(1S-trans)-N-[(3-Carboxy-1,2-
,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenyla-
lanine methyl ester (896.3 mg) in 10 mL of methanol. The solution
was stirred for 26 hours. The methanol is then removed in vacuo.
The aqueous layer is further diluted with water and extracted with
diethyl ether and the extract discarded. The aqueous layer is
acidified to pH=3-4 with 0.6N HCl resulting in a precipitate. The
precipitate is filtered washing with water to obtain
(1S-trans)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbo-
nyl]-4-[(2,6-dichlorobenzoyl)amino]-D-phenylalanine (692.2 mg).
Physical properties as follows: m.p. 145-150.degree. C.; .sup.1H
NMR (300 MHz, DMSO-d.sub.6). .delta. 12.3 (1H), 10.6 (1H), 7.51
(5H), 7.29 (1H), 7.18 (2H), 4.44 (1H), 3.07 (1H), 2.94 (1H), 2.25
(1H), 2.62 (1H), 194 (1H), 1.67 (1H), 1.28 (1H), 1.04 (3H), 0.99
(3H), 0.48 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI (m/z): 535
([M+H.sup.+]).
Preparation of Example 247
[0490] Scheme 37: 37-C-11 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1S-trans) and L-Phenylalanine
(1S-trans)-N-[(3-Carbox-
y-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-11 was
prepared as follows:
[0491] The preparation follows that of Preparation 37-C-10. The
starting materials are
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclo-
pentane-1-carboxylic acid, 36-F-1, and
4-[(2,6-dichlorobenzoyl)amino]-L-ph- enylalanine methyl ester,
hydrochloride salt, 37-B-1 Physical properties as follows: m.p.
145-153.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6). .delta.
12.3 (1H)l, 10.6 (1H), 7.51 (5H), 7.29 (1H), 7.18 (2H), 4.42 (1H),
3.01 (2H), 2.70 (1H), 1.88 (2H), 1.73 (1H), 1.36 (1H), 1.04 (3H),
0.94 (3H), 0.78 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI
(m/z): 535 ([M+H.sup.+]).
Preparation of Example 248
[0492] Scheme 37: 37-C-12 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1R-cis) and L-Phenylalanine
(1R-cis)-N-[(3-Carboxy-1,-
2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenyl-
alanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-12 was
prepared as follows:
[0493] The preparation follows that of Preparation 37-C-8. The
starting materials are:
(1R-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid 3-methyl
ester, 10-A, and 4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester, hydrochloride salt, 37-B-1. Physical properties as
follows: m.p. 154-160.degree. C. .sup.1H NMR (300 MHz,
MeOH-d.sub.4). .delta. 7.58 (2H), 7.42 (3H), 7.24 (3H), 4.74 (1H),
3.3 (1H), 3.03 (1H), 2.75 (1H), 2.41 (1H), 2.12 (1H), 1.80 (1H),
1.43 (1H), 1.14 (3H), 1.11 (3H), 0.63 (3H); MS-ESI (m/z): 533
([M-H.sup.-]); MS-ESI (m/z) 535 ([M+H.sup.+]).
Preparation of Example 249
Scheme 37: 37-C-13 where R.sup.3 is CH.sub.3, R.sup.1 is H, and the
stereochemistry is (1R-cis) and D-Phenylalanine
[0494]
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-
-dichlorobenzoyl)amino]-D-phenylalanine
(q2.sub.6H.sub.28Cl.sub.2N.sub.2O.- sub.6), 37-C-13 was prepared as
follows:
[0495] The preparation follows that of Preparation 37-C-8. The
starting materials are
(1R-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid 3-methyl
ester, 10-A, and 4-[(2,6-dichlorobenzoyl)amino]-D-phenylalanine
methyl ester, hydrochloride salt, 37-B-2. Physical properties as
follows: m.p. 155-159.degree. C.; .sup.1H NMR (300 MHz,
DMSO-d.sub.6). .delta. 12.4 (1H), 10.6 (1H), 7.51 (5H), 7.19 (3H),
4.42 (1H), 2.99 (2H), 2.64 (1H), 2.26 (1H), 1.94 (1H), 1.68 (1H),
1.29 (1H), 1.17 (3H), 1.05 (3H), 0.51 (3H); MS-ESI (m/z): 533
([M-H.sup.-].
Preparation of Example 250
[0496] Scheme 37: 37-C-14 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1R-trans) and L-Phenylalanine
(1R-trans)-N-[(3-Carbox-
y-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-14 was
prepared as follows:
[0497] The preparation follows that of Preparation 37-C-10. The
starting materials are
(1R-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclo-
pentane-1-carboxylic acid, 36-F-2, and
4-[(2,6-dichlorobenzoyl)amino]-L-ph- enylalanine methyl ester,
hydrochloride salt, 37-B-1 . . Physical properties as follows: m.p.
148-155.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6). .delta.
12.3 (2H), 10.6 (1H), 7.53 (5H), 7.27 (1H)f 7.21 (2H), 4.38 2 (1H),
3.03 (2H), 2.73 (1H), 1.92 (2H), 1.79 (1H), 1.43 (1H), 1.04 (3H),
0.75 (3H), 0.71 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI
(m/z): 535 ([M+H]).
Preparation of Example 251
[0498] Scheme 37: 37-C-15 where R.sup.3 is CH.sub.3, R.sup.1 is H,
and the stereochemistry is (1R-trans) and D-Phenylalanine
(1R-trans)-N-[(3-Carbox-
y-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-D-ph-
enylalanine (C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6), 37-C-15 was
prepared as follows:
[0499] The preparation follows that of Preparation 37-C-10. The
starting materials are
(1R-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclo-
pentane-1-carboxylic acid, 36-F-2, and
4-[(2,6-dichlorobenzoyl)amino]-D-ph- enylalanine methyl ester,
hydrochloride salt, 37-B-2.. Physical properties as follows: m.p.
134-140.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6). .delta.
12.3 (1H), 10.6 (1H), 7.50 (5H), 7.43 (1H), 7.21 (2H), 4.43 (1H),
3.03 (2H), 2.73 (1H), 1.92 (2H), 1.78 (1H), 1.41 (1H), 1.06 (3H),
0.96 (3H), 0.80 (3H); MS-ESI (m/z): 533 ([M-H.sup.-]); MS-ESI
(m/z): 535 ([M+H.sup.+]),
Preparation of Example 252
[0500] The synthesis for Example 252,
(1S-cis)-N-[(3-Carboxy-2,2,3-trimeth-
ylcyclopentyl)carbonyl]-3-bromo-4-[(2,
6-dichlorobenzoyl)amino]-L-phenylal- anine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6) is taught by Scheme 2
(Method B) as follows:
[0501] To a solution of
(1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxy- lic acid
1-(1,1-dimethylethyl) ester (153.9 mg), 15-D, in methylene chloride
(6 mL) at 0.degree. C. was added DIEA (1 mL), EDC (113.2 mg), HOET
(80.3 mg), and dimethylaminopyridine (20.1 mg). The reaction was
stirred at 0.degree. C. for 20 minutes and then
3-Bromo-4-[(2,6-dichlorob- enzoyl)amino]-L-phenylalanine methyl
ester hydrochloride salt (259.4 mg) was added as a solution in
methylene chloride (4 mL) and stirred at ambient temperature for 50
hours. The reaction was diluted with water and extracted with
methylene chloride. The extracts were washed with 0.5 N HCl, dried
over sodium sulfate and concentrated in vacuo. The crude material
was purified by flash chromatography over silica gel eluting with
AcOEt in hexane to obtain
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbon-
yl]-2,2,3-trimethylcyclopentyl]carbonyl]-3-bromo-4-[(2,6-dichlorobenzoyl)a-
mino]-L-phenylalanine methyl ester (153.8 mg). Physical properties
as follows: .sup.1H NMR (CDCl.sub.3) .delta. 8.33 (1H), 7.82 (1H),
7.31 (4H), 7.08 (1H), 5.87 (1H), 4.85 (1H), 3.74 (3H), 3.10 (2H),
2.52 (2H), 2.14 (1H), 1.73 (1H), 4.42 (10H), 1.23 (3H), 1.14 (3H),
0.79 (3H). MS-ESI (m/z): 681 ([M-H--I); MS-ESI (m/z): 683
([M+H.sup.+]).
[0502] The deprotection of the carboxylic acids follows that of
Examples 53, and 54 to obtain
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)ca-
rbonyl]-3-bromo-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine.
Physical properties as follows: m.p. 150-152.degree. C.; .sup.1H
NMR (CD.sub.3OD) .delta. 7.95 (1H), 7.67 (1H), 7.57 (1H), 7.45
(3H), 7.35 (1H), 4.73 (1H), 3.25 (1H), 2.99 (1H), 2.75 (1H), 2.52
(1H), 2.00 (1H), 1.70 (1H), 1.44 (1H), 1.24 (3H), 1.21 (3H), 0.79
(3H); MS-ESI (m/z): 611 ([M-H.sup.-]); MS-ESI (m/z): 613
([M+H.sup.+]). 104105
Preparation of Example 253
[0503]
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-
-dichloro-4-[(1,1-dimethyl)ethyl]benzoyl)amino]-L-phenylalanine
(C.sub.30H.sub.37Cl.sub.1N.sub.2O.sub.6).
[0504] 4-[(1,1-Dimethyl)ethyl]acetanilide (C.sub.12H.sub.17NO,
38-B)
[0505] To a solution of 4-tert-butylaniline 38-A (14.9 g, 99.8
mmol) and pyridine (11 mL, 0.14 mol) in CH.sub.2Cl.sub.2 (50 mL),
under N.sub.2 and at 0.degree. C., is added dropwise acetic
anhydride (12 mL, 0.13 mmol). The reaction mixture is stirred at rt
for 20 h, and is then quenched with 0.5 M aqueous HCl (100 mL). The
reaction mixture is extracted with CH.sub.2Cl.sub.2. The combined
CH.sub.2Cl.sub.2 extracts are washed with 0.5 M NaOH and brine, and
then are dried, filtered and concentrated to give 38-B as an
orange-colored solid (18.5 g). The solid is recrystallized from
MeOH/Heptane to give a white solid (10.3 g, 54%): mp 172-173 0C;
TLC (85:15 hexane/acetone) R.sub.f=0.19; .sup.13C NMR
(CD.sub.3OD).sub.--.delta. 171.48, 148.15, 137.19, 126.52, 121.03,
35.14, 31.81, 23.74; MS (+ESI) m/z 190.
[0506] N,2-Dichloro-4-[(1,1-dimethyl)ethyl]acetanilide
(C.sub.12H.sub.15Cl.sub.2NO, 38-C)
[0507] To a suspension of 38-B (17.5 g, 91.5 mmol) and anhydrous
NaOAc (19 g, 0.23 mmol) in HOAc (100 mL) under N.sub.2 at
10.degree. C. is added portionwise a solution of Cl.sub.2 (7 g) in
HOAc (100 ml). Upon complete addition (approximately 15 min) the
reaction mixture is allowed to warm to rt and is stirred for 1 h. A
second portion of Cl.sub.2 (approximately 8 g) in HOAc (100 mL) is
added and the resulting mixture is stirred at rt for 4.5 h.
Finally, Cl.sub.2 gas is bubbled directly into the stirred mixture
for 30 min. This mixture is stirred at rt for 17 h. It is
concentrated under reduced pressure, chasing the residual HOAc with
two portions of toluene. The solid is dissolved in EtOAc. The
solution is filtered to give, after evaporation, an orange-colored
oil (24.7 g). The oil is purified by silica chromatography (90:10
heptane/EtOAc) to give an orange-colored oil (18.4 g) that is
recrystallized from pentane to give 38-C as a white solid (12.8 g,
54%): mp 64-65.degree. C.; TLC (90:10 heptane/EtOAc) R.sub.f=0.21;
.sup.13C NMR (CDCl.sub.3).sub.--.delta. 167.84, 155.65, 137.57,
133,57, 130.23, 127.91, 125.61, 35.11, 31.03, 21.52; MS (FAB) m/z
228, 226.
[0508] 2-Chloro-4-[(1,1-dimethyl) ethyl]acetanilide
(C.sub.12H.sub.16Cl.sub.1NO, 38-D).
[0509] To a solution of 18.6 g (71.5 mmol) of 38-C in absolute EtOH
(100 mL) is added 10 M NaOH (7.1 mL) An exothermic reaction ensues.
After the temperature had moderated the mixture is heated at reflux
for 1 h. The pH of the cooled mixture is adjusted to pH 7-8 with
concentrated HCl. The resulting mixture is partially concentrated
(to remove EtOH), and then is diluted with CH.sub.2Cl.sub.2 and and
brine. The CH.sub.2Cl.sub.2 layer is separated. The aqueous
solution is extracted twice additionally with CH.sub.2Cl.sub.2. The
combined CH.sub.2Cl.sub.2 extracts are dried, filtered and
concentrated to brown-colored oil. The oil is purified by silica
chromatography (steps of 90:10 and 85:15 heptane/acetone) to give a
solid (12.6 g), that is recrystallized from MeOH/pentane to give
38-D (7.49 g, 47%) as a white solid: mp 152-153.degree. C.; TLC
(85:15 heptane/acetone) R.sub.f=0.33; .sup.13C NMR
(CDCl.sub.3).sub.--.delta. 168.23, 148.25, 131.92, 125.89, 124.70,
122.59, 121.64, 34.49,31.17, 24.70; MS (FAB) m/z 226.0995.
[0510] 2-Chloro-4-[(1,1-dimethyl)ethyl]aniline(C.sub.10H.sub.14ClN,
38-E).
[0511] To a suspension of 38-D (6.0 g, 26 mmol) in EtOH (90 mL) is
added 10 N NaOH (10 mL). The resulting mixture is heated at reflux.
The suspended solid dissolves gradually. After 17 h at reflux the
solution is cooled to 0.degree. C. and is neutralized to pH 7 with
concentrated HCl. The mixture is concentrated partially (to remove
EtOH). the resulting aqueous mixture is diluted with brine, and is
extracted with five portions of CH.sub.2Cl.sub.2. The combined
CH.sub.2Cl.sub.2 extracts are dried, filtered and concentrated to
give 38-E as an orange-colored oil (5.5 g): TLC (85:15
heptane/acetone)=0.53; .sup.1H NMR (CDCl.sub.3).sub.--.delta. 7.25
(1H), 7.09 (1H), 6.72 (1H), 3.82 (2H), 1.27 (9H); MS (+ESI; MeOH)
m/z 186, 184.
[0512] 2-Chloro-4-[(1,1-dimethyl)ethyl]benzonitrile
(C.sub.11H.sub.12ClN, 38-F).
[0513] To a solution of aniline 38-E (5.5 g, 30 mmol) in 10:6
HOAc/H.sub.2O (32 mL) is added concentrated H.sub.2SO.sub.4 (4.7
mL, 85 mmol). The brown-colored solution is cooled to 10.degree. C.
and is treated dropwise with a solution of NaNO.sub.2 (2.3 g, 33
mmol) in H.sub.2O (5 mL). After this addition is complete the
reaction mixture is stirred at 10.degree. C. for 1 h, yielding a
yellow-colored solution. During this time a solution of KCN (9.8 g,
150 mmole) in H.sub.2O (25 mL) is added to a cold (ice bath),
mechanically stirred solution of CuSO.sub.4.5H.sub.2O (9.0 g, 36
mmol) in H.sub.2O (25 mL). To this mixture is added NaHCO.sub.3 (20
g, 0.24 mmol) and benzene (30 mL), and the entire mixture is heated
to 50-55.degree. C. to dissolve all of the solids. The this
solution is added dropwise the solution of the diazonium salt over
20 min under N.sub.2 and at 50-55.degree. C. The reaction mixture
is kept for 30 min at 50-55.degree. C. for 0.5 h after the
addition. The mixture is cooled, and extracted thrice with benzene.
The combined benzene extracts are washed with 1N NaOH and brine,
and then dried, filtered and concentrated to give a reddish-brown
oil (6.8 g). The oil is purified by silica flash chromatography
(steps of 95:5 and 90:10 heptane/CH.sub.2Cl.sub.2) to give 38-F
(2.4 g, 41's): TLC (75:25 heptane/CH.sub.2Cl.sub.2) f=0.31; .sup.1H
NMR (CDCl.sub.3).sub.--.delta. 7.58 (1H), 7.49 (1H), 7.37 (1H),
1.31 (9H); .sup.13C NMR (CDCl.sub.3).sub.--.delta. 158.53, 136.59,
133.64, 127.22, 124.49, 116.29, 110.21, 35.49, 30.80.
[0514] 2-Chloro-4-[(1,1-dimethyl)ethyl]benzoic acid
(C.sub.11H.sub.13ClO.sub.2, 38-G).
[0515] A solution of 38-F (2.28 g, 11.8 mmol), H.sub.2O (7.4 mL),
10 N NaOH (5.9 mL), and 30. H.sub.2O.sub.2 (6.7 mL) in EtOH (80 mL)
is refluxed for 28 h. The solution is cooled to 0.degree. C. and
neutralized to pH 7 with concentrated HCl.
[0516] A solution of NaHSO.sub.3 (7 g), dissolved in the minimal
amount of H.sub.2O, is added. The reaction mixture is concentrated
partially (to remove most of the EtOH), basified to pH 12 with 1 N
NaOH, and extracted twice with CH.sub.2Cl.sub.2. The combined
CH.sub.2Cl.sub.2 extracts are discarded. The aqueous solution is
acidified with concentrated HCl to pH 3, and then is extracted with
CH.sub.2Cl.sub.2. The combined CH.sub.2Cl.sub.2 extracts are dried,
filtered and concentrated to give 38-G (2.07 g, 83%) as a white
crystalline solid: .sup.1H NMR 11.62 (1H), 8.02 (1H), 7.51 (1H),
7.39 (1H), 1.36 (9H); .sup.13C NMR (CDCl.sub.3).sub.--.delta.
171.17, 158.05, 134.84, 132.58, 128.75, 125.25, 123.96, 35.18,
30.91; MS (+ESI) m/z 237, 235 [M+Na].sup.+; MS (-ESI; MeOH) m/z
213, 211.
[0517]
(1S-cis)-N-[[3-(tert-Butoxycarbonyl)-2,2,3-trimethylcyclopentyl]car-
bonyl]-4-((2,6-dichloro-4-tert-butylbenzoyl)amino]-L-phenylalanine
Methyl Ester (C.sub.35H.sub.47ClN.sub.2O.sub.6, 38-H).
[0518] A mixture of
(1S-cis)-N-[[3-(tert-Butoxycarbonyl)-2,2,3-trimethylcy-
clopentyl]-carbonyl]-4-nitro-L-phenylalanine methyl ester (406 mg,
0.88 mmol) and 10% Pd/C (39 mg) in 1:1 MeOH:THF (10 mL) is
hydrogenated (30 psi H.sub.2) for 1 h. The reaction mixture is
filtered and concentrated to give the aniline as a colorless oil.
This aniline is coupled directly with acid 15-D (190 mg, 0.89
mmol), as described by the general procedure for the synthesis of
intermediates 7-F, to give after silica flash chromatography (steps
of 99:1, 98:2, and 98:3 CHCl.sub.3/Acetone) to give 38-H (224 mg,
41%): TLC (95:5 CHCl.sub.3/Acetone) R.sub.f=0.52; .sup.13C NMR
(CDCl.sub.3) .delta. 175.02, 172.53, 172.14, 164.53, 155.92,
136.86, 132.21, 131.96, 130.38, 130.31, 129.84, 127.48,
124.52,120.26, 80.19, 56.69, 54.44, 53.11, 52.36, 46.39, 37.20,
35.03, 32.36, 31.00, 28.07, 22.98, 22.48, 21.98, 20.61; MS (FAB)
m/z 627.3201.
[0519]
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-
-dichloro-4-[(1,1-dimethyl)ethyl]benzoyl)amino]-L-phenylalanine
(C.sub.30H.sub.37C.sub.1N.sub.2O.sub.6, Example 253). A solution of
38-H (101 mg, 0.16 mmol) in TFA (2 mL) is stirred at rt for 2 h.
The solution is diluted with CH.sub.2Cl.sub.2 and concentrated
thrice under reduced pressure. The residue is diluted with toluene
and again concentrated under reduced pressure to an oil. The oil is
dissolved in MeOH (1.0 mL) and then treated with H.sub.2O (0.65 mL)
and 1.00 M LiOH 0.35 mL). After 16 h a second portion of 1.00 M
LiOH is added and the hydrolysis is allowed to proceed for an
additional 4 h. The solution was diluted with H.sub.2O and the pH
adjusted to ca. 8-9. The neutralized solution is diluted with MeOH
and then concentrated. The aqueous concentrate is diluted with
additional H.sub.2O, basified to pH 13 (1N NaOH), and extracted
with Et.sub.2O. The Et.sub.2O extract is discarded. The aqueous
phase is acidified to pH 2 (conc. HCl) and is extracted with EtOAc.
The combined EtOAc extracts are dried, filtered and concentrated to
give Example 253 (93 mg) as a colorless oil: .sup.1H NMR
(CD.sub.3OD) .delta. 7.61 (2H), 7.55-7.44 (3H), 7.25 (2H),
4.69-4.78 (1H), 3.23 (1H), 3.00 (1H), 2.80-2.70 (1H), 2.60-2.46
(1H), 2.10-1.94 (1H), 1.78-1.61 (1H), 1.52-1.39 (1H), 1.36 (9H);
1.27 (3H), 1.23 (3H), 0.81 (3H); MS (+ESI) m/z 557, 555. 106107
Preparation of Example 254
[0520]
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcy-
clopentyl)carbonyl]amino]-3-[(2,6-dichlorophenyl)methoxy]-6-pyridinepropan-
oic acid (C.sub.25H.sub.28Cl.sub.2N.sub.2O.sub.6).
[0521] (.+-.)-2-Chloro-3-[(2-tetrahydropyranyl)oxyl-6-iodopyridine
(C.sub.10H.sub.11ClINO.sub.2, 39-A):
[0522] To a solution of chloroiodopyridinol 34-B (1.00 g, 3.91
mmol) and dihydropyran (1.0 mL, 10.6 mmol) in CH.sub.2Cl.sub.2 (10
mL) under Ar at rt is added pyridinium chloride (0.050 g). The
reaction mixture is stirred for 72 h. It is diluted with
CH.sub.2Cl.sub.2, and is washed with satd aq NaHCO.sub.3 and brine.
The CH.sub.2Cl.sub.2 solution is dried, filtered and concentrated
to an oil, that is purified by silica flash chromatography (19:1
hexanes/EtOAc) to give 1.06 g (3.12 mmol, 80%) of 39-A: TLC (19:1
hexanes/EtOAc) R.sub.f=0.24; .sup.1H NMR (CDCl.sub.3, 300 MHz)
.delta..sub.--7.55 (1H), 7.17 (1H), 5.50 (1H), 3.77 (1H), 3.61
(1H), 2.07-1.57 (6H); MS (+ESI) m/z 361.9, 339.9.
[0523]
(2S)-2-Chloro-.alpha.-[[(1,1-dimethylethoxy)carbonyl]amino]-3-[(2-t-
etrahydropyranyl)-oxy]-6-pyridinepropanoic Acid Methyl Ester
(C.sub.19H.sub.27ClN.sub.2O.sub.6, 39-B):
[0524] To an amberized flask containing Reference Example 57 (1.81
g, 5.52 mmol) and activated Zn dust (0.349 g, 5.51 mmol) under Ar
is added THF (2 mL) and 1,2-dibromoethane (0.018 mL, 0.21 mmol).
The suspension is brought to reflux for several minutes, cooled to
approximately 30.degree. C., and TMSCl (0.17 mL of a 1 M solution
in THF) is added. The reaction mixture is stirrred at
40.+-.5.degree. C. for 30 min, cooled in an ice bath, and solid
PdCl.sub.2 (PPh.sub.3).sub.2 (0.192 g) is added. A degassed
solution of the iodide 39-A (0.936 g, 2.76 mmol) in 1:1
THF/dimethylacetamide (5.6 mL) is added. This reaction mixture is
stirred for 4 h at 45.+-.5.degree. C. It is then cooled to
0.degree. C., quenched with satd aq NH.sub.4Cl, and extracted with
EtOAc. The combined EtOAc portions are washed with satd aq
NH.sub.4Cl and brine, and are dried, filtered and concentrated to a
green-yellow colored foam. This foam is purified by silica flash
chromatography (7:3 hexanes/EtOAc) to give 0.879 g (1.85 mmol, 60%)
of 39-B: TLC (7:3 hexanes/EtOAc) R.sub.f 0.21; .sup.1H NMR
(CDCl.sub.3, 300 MHz) .delta..sub.--7.39 (1H), 7.00 (1H), 5.46
(1H), 4.61 (1H), 4.13 (11H), 3.80 (3H), 3.62 (1H), 3.20 (1H),
2.13-1.53 (6H), 1.42 (9H); MS (+ESI) m/z 474.0
[0525]
(S)-.alpha.-[[(1,1-Dimethylethoxy)carbonyl]amino]-3-[(2-tetrahydrop-
yranyl)oxy]-6-pyridinepropanoic Acid Methyl Ester
(C.sub.19H.sub.28N.sub.2- O.sub.6, 39-C):
[0526] A suspension of pre-reduced Pd/CaCO.sub.3 (3.5 g) and 39-B
(1.15 g, 2.77 mmol) in EtOH (40 mL) is hydrogenated (30 psi
H.sub.2) for 19 h at rt. The mixture is filtered, and the filtrate
is evaporated to give a yellow-colored foam that is purified by
silica flash chromatography (600:400:1 hexanes/EtOAc/iPrOH) to give
0.367 g (0.96 mmol, 35%) of 39-C: TLC (1:1 hexanes/EtOAc) R.sub.f
0.27; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta..sub.--8.30 (1H),
7.29 (1H), 7.03 (1H), 5.81 (1H), 5.39 (1H), 4.65 (1H), 3.86 (1H),
3.73 (3H), 3.62 (1H), 3.21 (2H), 1.96-1.53 (6H), 1.42 (9H); MS
(+ESI) m/z 381.1.
[0527]
(S)-.alpha.-[[(1,1-Dimethylethoxy)carbonyl]amino]-5-hydroxy-2-pyrid-
inepropanoic Acid Methyl Ester (C.sub.14H.sub.20N.sub.2O.sub.5,
39-D):
[0528] A solution of 39-C (0.346 g, 0.91 mmol) and pyridinium
p-toluenesulfonate (0.031 g, 0.12 mmol) in EtOH (8 mL) is stirred
at 55.+-.5.degree. C. for 20 h. The reaction mixture is cooled to
rt, and concentrated in vacuo. The residue is taken up in EtOAc
(150 mL). This solution is washed with brine, dried, filtered and
concentrated to a pale yellow-colored oil that is purified by
silica flash chromatography (500:500:1 hexanes/EtOAc/iPrOH).
Evaporation of the column fractions gives recovered 39-C (0.27
mmol) and 0.132 g (0.45 mmol, 49%) of 39-D: TLC (1:1 hexanes/EtOAc)
R.sub.f=0.18; .sup.1H NMR (CDCl.sub.3, 300 MHz) .delta..sub.--8.13
(1H), 7.13 (1H), 7.03 (1H), 5.71 (1H), 4.65 (1H), 3.70 (3H), 3.20
(2H), 1.39 (9H); MS (+ESI) m/z 297.1.
[0529]
(S)-5-[(2,6-Dichlorophenyl)methoxy]-.alpha.-[((1,1-dimethylethoxy)c-
arbonyl]amino]-2-pyridinepropanoic Acid Methyl Ester
(C.sub.21H.sub.4Cl.sub.2N.sub.2O.sub.5, 39-E):
[0530] To a solution of 39-D (0.126 g, 0.43 mmol),
2,6-dichlorobenzylalcoh- ol (0.075 g, 0.43 mmol) and PPh.sub.3
(0.113 g, 0.43 mmol) in dry THF (4 mL) at 0.degree. C. under Ar is
added DEAD (0.068 mL). The reaction mixture is permitted to warm to
rt, and is stirred for 18 h. It is concentrated. The residue is
purified by silica flash chromatography (700:300:1
hexanes/EtOAc/iPrOH) to give 0.149 g (0.33 mmol, 76%) of 39-E: TLC
(7:3 hexanes/EtOAc) R.sub.f=0.34; .sup.1H NMR (CDCl.sub.31 300 MHz)
.delta..sub.--8.31 (1H), 7.37 (2H), 7.25 (2H), 7.08 (1H), 5.81
(1H), 5.29 (2H), 4.65 (1H), 3.70 (3H) 3.24 (2H), 1.63 (1H) 1.43
(9H); .sup.13C NMR (CDCl.sub.3, 75 MHz) .delta..sub.--172.47,
155.50, 153.82, 149.71, 137.33, 137.00, 131.51, 130.72, 128.56,
123.99, 122.78, 79.74, 65.64, 53.25, 52.27, 38.43, 28.33; MS (+ESI)
m/z 454.9.
[0531]
(S)-.alpha.-Amino-5-[(2,6-dichlorophenyl)methoxy]-2-pyridinepropano-
ic Acid Methyl Ester Dihydrogen Chloride Salt
(C.sub.16H.sub.16Cl.sub.2N.s- ub.2O.sub.3.2HCl, 39-F):
[0532] A solution of carbamate 39-E (0.546 g, 1.20 mmol) in 4 M HCl
in dioxane (12 mL) is stirred at rt under Ar for 16 h. The reaction
mixture is concentrated in vacuo. The residue is dissolved in
H.sub.2O (40 mL), and this solution is extracted with Et.sub.2O.
The aqueous solution is frozen and lyophilized to give 0.485 g
(1.13 mmol, 94%) of 39-F as a light yellow-colored solid: .sup.1H
NMR (CD.sub.3SOCD.sub.3, 300 MHz) .delta..sub.--8.75 (3H), 8.47
(1H), 7.81 (1H), 7.57 (3H), 7.48 (1H), 5.35 (2H), 4.49 (1H), 3.67
(3H), 3.42 (2H); .sup.13C NMR (CD.sub.3SOCD.sub.3, 75 MHz)
.delta..sub.--169.42, 154.95, 146.54, 136.57, 134.35, 132.50,
131.30, 129.36, 126.72, 126.52, 66.40, 53.32, 51.79, 34.81.
[0533]
[1S-[1.alpha.(R*),3.alpha.]]-3-[(2,6-Dichlorophenyl)methoxy]-.alpha-
.-[[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-
amino]-6-pyridinepropanoic Acid Methyl Ester
(C.sub.3H.sub.38Cl.sub.2N.sub- .2O.sub.6, 39-G):
[0534] To a mixture of 15-D (0.141 g, 0.55 mmol), EDC (0.108 g,
0.57 mmol), HOBt (0,079 g, 0.58 mmol), DMAP (0.020 g, 0.16 mmol)
and amine 39-F (0.246 g, 0.57 mmol) in CH.sub.2Cl.sub.2 (6 mL) at
0.degree. C. under Ar is added Et.sub.3N (0.18 mL, 1.26 mmol). The
yellow-colored reaction mixture is stirred at rt for 90 h. It is
diluted with CH.sub.2Cl.sub.2, and washed with H.sub.2O, 0.5 M aq
HCl, H2O, satd aq NaHCO.sub.3, and H.sub.2O. The CH.sub.2Cl.sub.2
solution is dried, filtered and concentrated to a pale
yellow-colored foam, that is purified by silica flash
chromatography (600:400:1 hexanes/EtOAc.iPrOH) to give 0.195 g
(0.33 mmol, 60%) of 39-G: TLC (3:2 hexanes/EtOAc) R.sub.f=0.49;
.sup.1H NMR (CDCl.sub.3, 300 MHz) .delta. 8.28 (1H), 7.40-7.28
(4H), 7.13 (1H), 5.31 (2H), 4.92 (1H), 3.68 (3H), 3.33 (1H), 3.25
(1H), 2.65 (1H), 2.53 (1H), 2.21 (1H), 1.67 (1H), 1.44 (9H, 1.31
(3H), 1.18 (3H), 0.83 (3H).
[0535]
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcy-
clopentyl)carbonyl]amino]-3-[(2,6-dichlorophenyl)methoxy3-6-pyridinepropan-
oic Acid Methyl Ester (C.sub.2H.sub.30Cl.sub.2N.sub.2O.sub.6,
39-H):
[0536] A solution of diester 39-G (0.195 g, 0.33 mmol) in TFA (4
mL) under Ar is stirred at 0.degree. C. for 1 h and at rt for 2 h.
The solution is concentrated, azeotroped thrice from toluene, and
dried under vacuum to give 39-H as a yellow-colored glass: TLC
(400:600:5 hexanes/EtOAc/HCO.sub.2H) 4-0.29; .sup.1H NMR
(CDCl.sub.3, 300 MHz) .delta..sub.--8.46 (1H), 7.87 (1H), 7.74
(1H), 7.51 (1H), 7.42-7.26 (2H), 5.42 (2H), 5.01 (1H), 3.80 (3H),
3.62 (1H), 3.45 (1H), 2.70 (1H), 2.48 (1H), 1.97 (1H), 1.65 (1H),
1.49 (1H), 1.28 (3H), 1.24 (3H), 0.76 (3H).
[0537]
[1S-[1.alpha.(R*)-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopentyl)c-
arbonyl]amino)-3-[(2,6-dichlorophenyl)methoxy]-6-pyridinepropanoic
acid (C.sub.5H.sub.2Cl.sub.2N.sub.2O.sub.6, Example 254):
[0538] A solution of 39-H (0.33 mmol) and LiOH.H.sub.2O (0.068 g,
1.62 mmol) in 2:1 THF/H.sub.2O (10.5 mL) is stirred under Ar for 5
h at rt. The reaction mixture is diluted with cold H.sub.2O,
acidified with aq 1 M HCl, and extracted with EtOAc. The combined
EtOAc extracts are washed with brine, and are dried, filtered and
concentrated to a pale yellow-colored foam that is purified by
silica flash chromatography (600:400:2 hexanes/EtOAc/HCO.sub.2H).
The purified product is azeotroped thrice from toluene to remove
HCO.sub.2H. It is dissolved in MeCN (10 mL) and the solution is
diluted with H.sub.2O (10 mL). The solution is frozen and
lyophilized to give, as an beige-colored solid, 0.170 g (0.32 mmol,
98%) of Example 254: mp 118-122.degree. C.; TLC (150:50:1
EtOAc/hexanes/HCO.sub.2H) R.sub.f=0.41;
[0539] .sup.1H NMR (CD.sub.3SOCD.sub.3, 300 MHz) .delta. 8.43 (1H),
7.86 (2H) 7.58-7.47 (3H), 7.32 (1H), 5.28 (2H), 4.64 (1H),
3.24-2.99 (2H), 2.61 (1H), 2.32 (1H), 1.83 (1H), 1.51 (1H), 1.30
(1H), 1.12 (3H), 1.09 (3H), 0.63 (3H).
EXAMPLES
Example 1
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-(phenylmethy-
l)-L-tyrosine methyl ester (C.sub.27H.sub.33NO.sub.6)
[0540] 108
[0541] DIEA (0.65 g) was added dropwise to a mixture of
(1R)camphoric anhydride (0.18 g) and O-benzyl-L-tyrosine methyl
ester hydrochloride (0.33 g) in DMF (2 ml) at 0.degree. C. The
mixture was stirred at 40.degree. C. for 15 hr, cooled, diluted
with AcOEt, and acidified with 1N HCl to pH 5. The organic layer
was washed with H.sub.2O, brine, dried over Na.sub.2SO.sub.4, and
the solvent was removed in vacuo. The residue was purified by
column chromatography on silica gel (eluent; 98:2, CHCl.sub.3/MeOH)
to give (1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopenty-
l)carbonyl]-O-(phenylmethyl)-L-tyrosine methyl ester
(C.sub.27H.sub.33NO.sub.6) (0.45 g) as a gum. MS (m/z): 468
(MH.sup.+)
Example 2
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-(phenylmethy-
l)-L-tyrosine (C.sub.26H.sub.31NO.sub.6)
[0542] 109
[0543] LiOH (72 mg) was added to a mixture of (1S-cis)
--N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-(phenylmethyl)-L-t-
yrosine methyl ester (C.sub.27H.sub.33NO.sub.6) (403 mg), THF (3
ml), and H.sub.2O (3 ml). The mixture was stirred at room
temperature for 4 hr, acidified with 1N HCl, and extracted with
AcOEt. The extract was dried over Na.sub.2SO.sub.4 and the solvent
was removed in vacuo to give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-(phenylmeth-
yl)-L-tyrosine (391 mg), mp 146-149.degree. C., MS (m/z): 452
([M-H]-).
Example 3
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-[(3,4-dichlo-
rophenyl)methyl]-L-histidine Methyl Ester
(C.sub.24H.sub.29Cl.sub.2N.sub.3- O.sub.5)
[0544] 110
[0545] DIEA (4.48 g) was added to a mixture of
1-(3,4-dichlorobenzyl)-L-hi- stidine methyl ester (5.56 g) and
(1R)-camphoric anhydride (2.53 g) in DMF (50 ml). The mixture was
stirred at 40.degree. C. for 17 hr, cooled, diluted with H.sub.2O,
acidified with 5% HCl to pH 5, and extracted with CHCl.sub.3. The
extract was washed with H.sub.2O, brine, and dried over NaSO.sub.4.
The solvent was removed in vacuo and the residue was purified by
flash chromatography on silica gel (eluent: 100:1,
CHCl.sub.3/MeOH), followed by recrystallization from AcOEt/MeOH to
give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-[(3,4-dichl-
orophenyl)methyl]-L-histidine methyl ester (5.90 g), mp
174-175.degree. C. (dec), MS (m/z): 510 (MH.sup.+).
Example 4
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-[(3,4-dichlo-
rophenyl)methyl]-L-histidine
(C.sub.23H.sub.27Cl.sub.2N.sub.3O.sub.5)
[0546] 111
[0547] 1N NaOH (7.3 ml) was added dropwise to a mixture of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-((3,4-dichl-
orophenyl)methyl]-L-histidine methyl ester
(C.sub.24H.sub.19Cl.sub.2N.sub.- 3O.sub.5) (1.50 g) in MeOH (20 ml)
at 0.degree. C. The mixture was stirred at room temperature for 15
hr, concentrated in vacuo, diluted with H.sub.2O, and acidified
with 1N HCl to pH 5-6. The resulting precipitate was collected by
filtration, washed with H.sub.2O, dried, and recrystallized from
DMF/H.sub.2O to give (1S-cis)-N-[(3-Carboxy-2,2,3-tri-
methylcyclopentyl)carbonyl]-1-[(3,4-dichlorophenyl)methyl]-L-histidine
(0.87 g), mp 148-149.degree. C. (dec), MS (m/z): 496 (MH.sup.+)
Example 5
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopent-
yl)carbonyl]amino]-2-naphthalenepropanoic Acid Phenylmethyl Ester
(C.sub.30H.sub.33NO.sub.5)
[0548] 112
[0549] Benzyl (S)-2-amino-3-(2-naphthyl) propionate tosylate (0.20
g) was partitioned between AcOH and sat. NaHCO.sub.3. The organic
layer was washed with H.sub.2O, brine, dried over Na.sub.2SO.sub.4,
and the solvent was removed in vacuo. DMF (5 ml) and (1R)-camphoric
anhydride (0.20 g) were added to the residue. The mixture was
stirred at 30-40.degree. C. for 17 hr, cooled, and poured into
H.sub.2O. The resulting mixture was extracted with AcOH. The
extract was washed with H.sub.2O, brine, dried over
Na.sub.2SO.sub.4, and the solvent was removed in vacuo. The residue
was purified by column chromatography on silica gel (eluent; 9:1,
CHCl.sub.3/AcOEt) to give
[1S-[1.alpha.(R*),3.alpha.]-.alpha.-[[(3-Carbox-
y-2,2,3-trimethylcyclopentyl)carbonyl]amino]-2-naphthalenepropanoic
acid phenylmethyl ester (0.51 g) as an oil. MS (m/z) 488
(MH.sup.+).
Example 6
(1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopent-
yl)carbonyl]amino]-2-naphthalenepropanoic Acid
(C.sub.23H.sub.27NO.sub.5)
[0550] 113
[0551] 10% Pd-C (0.05 g) was added to a solution of
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopen-
tyl)carbonyl]amino]-2-naphthalenepropanoic acid phenylmethyl ester
(C.sub.30H.sub.33NO.sub.5) (0.20 g) in MeOH (10 ml) and the mixture
was subjected to hydrogenolysis at a hydrogen pressure of 50 psi.
The catalyst was filtered off and the filtrate was evaporated in
vacuo to give
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyc-
lopentyl)carbonyl]amino]-2-naphthalenepropanoic acid (0.18 g), MS
(m/z): 398 (MH.sup.+).
[0552] Examples 7 through 51 were prepared in a similar manner as
described in Examples 1-6, and are shown in Tables 1, 2, and 3.
2TABLE 1 Examples 7 through 20: 114 Ex. physicochemical No. *
R.sup.0 R.sup.15 Z.sup.1 R.sup.13 R.sup.18 property 7 S H H single
t-BuO-- H M.P.: 98--100.degree. C. bond MS(m/z) 420 (MH.sup.+) 8 S
CH.sub.3 H --OCH.sub.2-- 115 H gum MS(m/z): 518 (MH.sup.+) 9 S H H
--OCH.sub.2-- 116 H M.P.: 92-93.degree. C. MS (m/z): 502 ([M -
H].sup.-) 10 S H H --OCH.sub.2-- 117 H MS (m/z): 504 (MH.sup.+) 11
S CH.sub.3 H --OCH.sub.2-- 118 H gum MS (m/z): 536 (MH.sup.+) 12 S
H H --OCH.sub.2-- 119 H M.P.: 95-97.degree. C. MS (m/z): 520 ([M -
H].sup.-) 13 S H H --OCH.sub.2-- 120 H M.P.: 102-105.degree. C. MS
(m/z): 460 (MH.sup.+) 14 S H H --OCH.sub.2-- 121 H M.P.:
94-97.degree. C. MS (m/z): 476 (MH.sup.+) 15 S H I --OCH.sub.2--
122 I M.P. 210-212.degree. C. (dec.) MS (m/z): 784, 786 (MH.sup.+)
16 S H H --OCH.sub.2-- 123 H MS (m/z): 520 ([M - H].sup.-) 17 S H
PhCH.sub.2O --OCH.sub.2-- Ph H MS (m/z): 558 ([M - H].sup.-) 18 S H
H --OCH.sub.2-- 124 H MS (m/z): 530 (MH.sup.+) 19 R CH.sub.3 H
--OCH.sub.2-- Ph H gum MS (m/z): 468 (MH.sup.+) 20 R H H
--OCH.sub.2-- Ph H MS (m/z): 454 (MH.sup.+)
[0553]
3TABLE 2 Examples 21 through 38: 125 Ex. physicochemical No.
R.sup.0 Z.sup.2 R.sup.16 property 21 CH.sub.3 single --C(Ph).sub.3
gum bond MS (m/z): 594 (MH.sup.+) 22 H single --C(Ph).sub.3 MS
(m/z): 58O (MH.sup.+) bond 23 CH.sub.3 CH.sub.2 126 gum MS (m/z):
510 (MH.sup.+) 24 H CH.sub.2 127 MS (m/z): 496 (MH.sup.+) 25
CH.sub.3 CH.sub.2 128 gum MS (m/z): 510 (MH.sup.+) 26 H CH.sub.2
129 MS (m/z) 496 (MH.sup.+) 27 CH.sub.3 CH.sub.2 130 gum MS (m/z):
476(MH.sup.+) 28 H CH.sub.2 131 MS (m/z): 462 (MH.sup.+) 29 H
CH.sub.2 Ph M.P. 258-259.degree. C. (dec.) MS (m/z): 428 (MH.sup.+)
30 CH.sub.3 CH.sub.2 132 gum MS (m/z): 472 (MH.sup.+) 31 H CH.sub.2
133 MS (m/z): 458 (MH.sup.+) 32 H CH.sub.2 PhCH.sub.2O MS (m/z):
458 (MH.sup.+) 33 CH.sub.3 CH.sub.2 134 gum MS (m/z): 460
(MH.sup.+) 34 H CH.sub.2 135 MS (m/z): 446 (MH.sup.+) 35 CH.sub.3
CH.sub.2 136 gum MS (m/z): 510 (MH.sup.+) 36 H CH.sub.2 137 MS
(m/z): 496 (MH.sup.+) 37 CH.sub.3 CH.sub.2 138 gum MS (m/z): 492
(MH.sup.+) 38 H CH.sub.2 139 MS (m/z): 478 (MH.sup.+)
Example 39
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlo-
rophenyl)methyl]-D-tyrosine (C.sub.26H.sub.29Cl.sub.2NO.sub.6)
[0554] 140
[0555] Example 39 is an isomer of Example 12 and is therefore
synthesized in a manner similar to that of Example 12.
4TABLE 3 Examples 40 through 51: 141 physicochemicali Ex. No. *
R.sup.0 R.sup.6 property 40 S H 142 MS(m/z): 452 (MH.sup.+) 41 S H
143 M.P.: 163-165.degree. C. MS (m/z): 354 (MH.sup.+) 42 S H 144 MS
(m/z): 398 (MH.sup.+) 43 S H 145 MS (m/z): 509 ([M - H.sup.-]) 44 S
H Ph MS (m/z): 348 (MH.sup.+( 45 S CH.sub.3 146 gum MS (m/z): 468
(MH.sup.+) 46 S H 147 M.P.: 88-90.degree. C. MS (m/z): 452 ([M -
H].sup.-) 47 S H 148 MS (m/z): 502 ([M - H].sup.-) 48 S H 149 MS
(m/z): 422 ([M - H].sup.-) 49 S H 150 MS (m/z): 475 ([M - H].sup.-)
50 S H 151 MS (m/z): 537 (MH.sup.+) 51 R H 152 MS (m/z): 364
(MH.sup.+)
Example 52
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine Methyl
Ester (C.sub.27H.sub.30Cl.sub.2N.sub.2O.sub.6)
[0556] 153
[0557] HCl gas was bubbled through a solution of
N-(tert-butoxycarbonyl)-4- -(2,6-dichlorobenzamido)-L-phenylalanine
(800 mg) in MeOH (15 ml) for 5 minutes and the mixture was stirred
for 3 hr. at room temperature. Excess HCl was removed by bubbling
N.sub.2 through the mixture and the solvent was removed in vacuo.
The residue was washed with ether and dried. To the resulting solid
was added THF (10 ml) containing DIEA (1.3 ml), BOP Reagent (938
mg) and (1S,3R)-3-(tert-butoxycarbonyl)-2,2,3-trimethylcyclo-
pentanecarboxylic acid (480 mg), which was prepared by the
saponification of methyl
(1S,3R)-3-(tert-butoxycarbonyl)-2,2,3-trimethylcyclopentanecarb-
oxylate derived from methyl (1S,3R)-3-carboxy (or
chlorocarbonyl)-2,2,3-tr- imethylcyclopentanecarboxylate and
t-BuOH. The mixture was stirred overnight under N, and the solvent
was removed in vacuo.
[0558] 1N HCl (10 ml) was added to the residue and the mixture was
extracted with AcOEt. The extract was washed with 1N HCl, brine;
sat. NaHCO.sub.3, brine, sat. LiCl, and brine, dried over
MgSO.sub.4, and evaporated in vacuo.
[0559] The residue was purified by column chromatography on silica
gel (eluent; 3:2, Hexane/AcOEt) to give
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)c-
arbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amin-
o]-L-phenylalanine methyl ester
(C.sub.27H.sub.30Cl.sub.2N.sub.2O.sub.6) (1.05 g) as a colorless
solid.
[0560] .sup.1H NMR (300 MHz, CDCl.sub.3), .delta. 7,58 (2H),
7.3-7,4 (3H), 7.11 (2H), 5.78 (1H), 4.8-5.0 (1H), 3.75 (3H), 3.10
(2H), 2.50 (1H), 2.0-2.2 (1H), 1.6-1.8 (1H), 1.44 (9H), 1.23 (3H),
1.16 (3H), 0.80 (3H); .sup.13C NMR (75 MHz, CDCl.sub.3), .delta.
175.07, 172.59, 172.17, 162.54, 136.32, 132.83, 132.48, 131.11,
130.01, 128.28, 120.56, 80.28, 56.76, 54.53, 53.12, 52.48, 46.46,
37.26, 32.41, 28.14, 23.06, 22.55, 22.06, 20.68; ESMS (m/z) 605
(MH.sup.+); Anal. Calcd for
C.sub.31H.sub.38Cl.sub.2N.sub.2O.sub.6.1/2H.sub.2O: C, 60.53; H.
6.35; N, 4.56; Found: C, 60.71; H, 6.31; N, 4.52. MS (m/z): 605
(MH.sup.+).
Example 53
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-L-phenylalanine Methyl Ester
(C.sub.27H.sub.30Cl.sub.2N.s- ub.2O.sub.6)
[0561] 154
[0562] TFA (1.5 ml) was added to a solution of
(1S-cis)-N-[[3-[(1,1-Dimeth-
ylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobe-
nzoyl)amino]-L-phenylalanine methyl ester
(C.sub.27H.sub.30Cl.sub.2N.sub.2- O.sub.6) (290 mg) in
CH.sub.2Cl.sub.2 (1.5 ml) and the mixture was stirred for 3 hr. The
solvent was removed in vacuo and the residue was triturated with
ether/CHCl.sub.3 to give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclo-
pentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (180 mg) as a colorless powder.
[0563] .sup.1H NMR (300 MHz, Acetone-d.sub.6), .delta. 9.75 (1H),
7.71 (2H), 7.4-7.6 (3H), 7.25 (2H), 7.12 (1H), 4.7-4.85 (1H), 3.68
(3H), 3.08 (2H), 2.85 (1H), 2.5-2.6 (1H), 1.6-1.8 (1H), 1.4-1.5
(1H), 1.26 (3H), 1.19 (3H), 0.80 (3H); .sup.13C NMR (75 MHz,
Acetone-dQ), .delta. 176.29, -172.20, 172.11, 162.00, 137.49,
133.12, 131.85, 131.12, 129.71, 128.16, 119.59, 55.98, 53.63,
52.89, 51.37, 46.14, 36.67, 32.47, 22.57, 22.04, 21.41, 20.74; ESMS
(m/z) 549 (MH.sup.+); Anal. Calcd for
C.sub.27H.sub.30Cl.sub.2N.sub.2O.sub.6.1/2H.sub.2O: C, 58.02; H,
5.55; N, 5.01; Found: C, 58.70; H, 5.53; N, 5.01. MS (m/z): 549
(MH.sup.+)
Example 54
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0564] 155
[0565] A solution of LiOH (19 mg) in H.sub.2O (1 ml) was added to a
solution of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-
-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine methyl ester
(C.sub.27H.sub.30Cl.sub.2N.sub.1O.sub.6) (108 mg) in THF (4
ml)/MeOH (1 ml). The mixture was stirred for 2 hr. acidified with
1N HCl (15 ml), and extracted with AcOEt. The extract was washed
with brine, dried over MgSO.sub.4, and evaporated in vacuo to give
(1S-cis)-N-[(3-Carboxy-2,2,3--
trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalan-
ine (80 mg), m.p. 231-233.degree. C.
[0566] .sup.1H NMR (300 MHz, Acetone-d.sub.6). .delta. 9.72 (1H),
7.71 (2H) 7.47-7.48 (3H), 7.27 (2H), 7.07 (1H), 4.78-4.85 (1H),
3.15 (2H), 2.85 (1H), 2.5-2.6 (1H), 1.6-1.8 (1H), 1.4-1.5 (1H),
1.26 (3H), 1.19 (3H), 0.81 (3H); .sup.13C NMR (75 MHz,
Acetone-d.sub.6), .delta. 177.67, 173.83, 173.62, 163.32, 138.74,
134.64, 133.17, 132.42, 131.10, 129.47, 120.88, 57.29, 54.69,
54.28, 47.47, 37.93, 33.81, 23.91, 23.39, 22.73, 22.08; ESMS (m/z)
535 (MH.sup.+), 533 (M-H).sup.-; Anal. Calcd for
C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6.1/2H.sub.2O: C, 57.46; H,
5.34; N, 5.15; Found: C, 57.48; H. 5.38; N, 5.15. MS (m/z): 533
([M-H.sup.-]); MS (m/z): 578 (MH.sup.+).
Example 55
(1S-cis)-N-[[3[(1,1
Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]ca-
rbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
[0567] 156
[0568] LiOH ('177 mg) in H.sub.2O (10 ml) was added to a mixture of
(1S-cis)-N-[(3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl)-
carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine methyl ester
(2.18 g) in THF (10 ml)/MeOH (5 ml) and the mixture was stirred at
room temperature for 3 hr, acidified with 1N HCl (20 ml), and
extracted with AcOEt. The extract was dried over Na.sub.2SO.sub.4
and the solvent was removed in vacuo to give
(1S-cis)-N-[(3-[(1,1-Dimethylethoxy)carbonyl]-2,-
2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosi-
ne (2.07 g). MS (m/z): 578 (MH.sup.+).
Example 56
(1S-cis)-4-Amino-N-[[3-[(1,1-,
dimethylethoxy)carbonyl]-2,2,3-trimethylcyc-
lopentyl]carbonyl]-L-phenylalanine Methyl Ester
[0569] 157
[0570] Two methods for the preparation of Example 56 are taught
according to Scheme 5a and 5b.
Example 57
(1S-cis)-N-[[3-[(1,1
Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-4-[(2,5-dichlorobenzoyl)amino]-L-phenylalanine Methyl
Ester
[0571] 158
[0572] BOP-Cl (425 mg) and 2,5-dichlorobenzoic acid (319 mg) were
added to a mixture of
(1S-cis)-4-Amino-N-L[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3--
trimethylcyclopentyl]carbonyl]-L-phenylalanine methyl ester (433
mg) and DIEA (0.7 ml) in CH.sub.2Cl.sub.2 (5 ml). The mixture was
stirred for 3 hr at room temperature, acidified with 1N HCl (50 ml)
and extracted with CH.sub.2Cl.sub.2. The extract was dried over
Na.sub.2SO.sub.4 and evaporated in vacuo. The residue was purified
by column chromatography on silica gel (eluent; Hexane 50%
Hexane/AcOEt) to give
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]-
carbonyl]-4-[(2,5-dichlorobenzoyl)amino]-L-phenylalanine methyl
ester (804 mg). MS (m/z): 605 (MH.sup.+).
Example 58
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-4-[(2,4,6-trichlorobenzoyl)amino]-L-phenylalanine Methyl
Ester
[0573] 159
[0574] The preparation of Example 58 is taught by Scheme 13.
Example 59
(1S-cis)-4-Acetylamino-N-[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethy-
lcyclopentyl]carbonyl]-L-phenylalanine Methyl Ester
[0575] 160
[0576] Acetic anhydride (1 ml) was added to a mixture of
(1S-cis)-4-Amino-N-[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcycl-
opentyl]carbonyl]-L-phenylalanine methyl ester (710 mg) and DIEA (4
ml) in CH.sub.2Cl.sub.2 (5 ml). The mixture was stirred for 30
minutes at room temperature and partitioned between sat.
NaHCO.sub.3 and AcOEt. The organic layer was dried over
Na.sub.2SO.sub.4 and the solvent was removed in-vacuo. The residue
was purified by column chromatography on silica gel (eluent;
Hexane.fwdarw.EtOH/AcOEt (1:1)) to give (1S--cis)-4-Acetylamino--
N-[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]--
L-phenylalanine methyl ester (669 mg). MS (m/z): 475
(MH.sup.+).
[0577] Examples 60-153 were prepared in a similar manner, as
described in Examples 52-59, and are shown in Table 4.
5TABLE 4 Examples 60 through 153: 161 physico- Ex. chemical No.
R.sup.19 R.sup.0 R.sup.15 Z.sup.1 R.sup.13 property 60 H CH.sub.3
162 single bond OH gum MS (m/z): 565 (MH.sup.+) 61 H H 163 single
bond OH M.P.: 168-171.degree. C. MS (m/z): 549 ([M - H].sup.-) 62 H
CH.sub.3 NO.sub.2 --OCH.sub.2-- 164 gum MS (m/z): 581 (MH.sup.+) 63
H H NO.sub.2 --OCH.sub.2-- 165 M.P.: 92-94.degree. C. MS (m/z): 565
([M - H.sup.-) 64 H CH.sub.3 OH --OCH.sub.2-- 166 gum MS (m/z): 552
(MH.sup.+) 65 H H OH --OCH.sub.2-- 167 MS (m/z): 538 (MH.sup.+) 66
H H 168 --OCH.sub.2-- 169 M.P.: 118-121.degree. C. MS (m/z): 696
(MH.sup.+) 67 H H 170 single bond OH M.P.: 236-238.degree. C. MS
(m/z): 538 (MH.sup.+) 68 H H NO.sub.2 single OH M.P.: 122- bond
125.degree. C. MS (m/z): 407 ([M - H].sup.-) 69 H H CH.sub.3CO
single OH M.P: 105- bond 108.degree. C. MS (m/z): 404 ([M -
H].sup.-) 70 H CH.sub.3 171 --OCH.sub.2-- 172 gum MS (m/z): 710
(MH.sup.+) 71 H CH.sub.3 NO.sub.2 single OH gum bond MS (m/z): 423
(MH.sup.+) 72 H CH.sub.3 H --NHCO-- PhCH.sub.2O gum MS (m/z): 511
(MH.sup.+) 73 H H H --NHCO-- PhCH.sub.2O M.P.: 95- 97.degree. C. MS
(m/z): 495 ([M - H].sup.-) 74 H CH.sub.3 H --NHCO-- 173 gum MS
(m/z): 487 (MH.sup.+) 75 H H H --NHCO-- 174 M.P.: 151-154.degree.
C. MS (m/z): 471 ([M - H].sup.-) 76 H CH.sub.3 H --NHCO-- 175 gum
MS (m/z): 482 (MH.sup.+) 77 H H H --NHCO-- 176 M.P.:
102-104.degree. C. MS (m/z): 466 ([M - H].sup.-) 78 H CH.sub.3 H
--NHCO-- CH.sub.3 gum MS (m/z): 419 (MH.sup.+) 79 H H H --NHCO--
CH.sub.3 M.P. 238- 240.degree. C. MS (m/z): 403 ([M - H].sup.-) 80
H CH.sub.3 H --NHCO-- 177 gum MS (m/z): 509 (MH.sup.+) 81 H H H
--NHCO-- 178 M.P.: 195-198.degree. C. MS (m/z): 495 (MH.sup.+); MS
(m/z): 493 ([M - H].sup.-) 82 H CH.sub.3 H --NHCO-- 179 gum MS
(m/z): 517 (MH.sup.+) 83 H H H --NHCO-- 180 M.P.: 150-152.degree.
C. MS (m/z): 503 (MH.sup.+) 84 H CH.sub.3 H --NHCO-- Ph gum MS
(m/z): 481 (MH.sup.+) 85 H H H --NHCO-- Ph M.P.: 145- 148.degree.
C. MS (m/z): 465 ([M - H].sup.-) 86 H CH.sub.3 H --NHCO-- 181 gum
MS (m/z): 549 (MH.sup.+) 87 H H H --NHCO-- 182 M.P.:
155-158.degree. C. MS (m/z): 533 ([M - H].sup.-) 88 H CH.sub.3 H
--NHCO-- 183 gum MS (m/z): 585 (MH.sup.+) 89 H H H --NHCO-- 184
M.P.: 190-193.degree. C. MS (m/z): 569 ([[M - H].sup.-] 90 H
CH.sub.3 H --NHCO-- 185 gum MS (m/z): 526 (MH.sup.+) 91 H H H
--NHCO-- 186 M.P.: 146-149.degree. C. MS (m/z): 512 (MH.sup.+) 92 H
CH.sub.3 H --NHCO-- 187 gum MS (m/z): 583 (MH.sup.+) 93 H H H
--NHCO-- 188 MS (m/z): 569 ([M - H].sup.-) 94 H CH.sub.3 H --NHCO--
189 gum MS (m/z): 511 (MH.sup.+) 95 H H H --NHCO-- 190 M.P.:
140-143.degree. C. MS (m/z): 497 (MH.sup.+) 96 H CH.sub.3 H
--NHCO-- 191 gum MS (m/z): 637 (MH.sup.+) 97 H H H --NHCO-- 192
M.P.: 170-173.degree. C. MS (m/z): 623 ([M - H].sup.-) 98 t-Bu
CH.sub.3 H --NHCO-- 193 gum MS (m/z): 589 (MH.sup.+) 99 H CH.sub.3
H --NHCO-- 194 gum MS (m/z): 533 (MH.sup.+) 100 H H H --NHCO-- 195
M.P.: 165.degree. C. (dec.) MS (m/z): 517 ([M - H].sup.-) 101 H
CH.sub.3 H --NHCO-- 196 gum MS (m/z): 526 (MH.sup.+) 102 H H H
--NHCO-- 197 MS (m/z): 512 (MH.sup.+) 103 H CH.sub.3 H --NHCO-- 198
gum MS (m/z): 617 (MH.sup.+) 104 H H H --NHCO-- 199 M.P.:
148-150.degree. C. MS (m/z): 601 ([M - H].sup.-) 105 H CH.sub.3 H
--NHCO-- 200 gum MS (m/z): 529 (MH.sup.+) 106 H H H --NHCO-- 201
M.P.: 165-168.degree. C. MS (m/z): 513 ([M - H].sup.-) 107 H
CH.sub.3 H --NHCO-- 202 gum MS (m/z): 549 (MH.sup.+) 108 H H H
--NHCO-- 203 M.P.: 180-183.degree. C. MS (m/z0: 533 ([M - H].sup.-)
109 H CH.sub.3 H --NHCO-- 204 gum MS (m/z): 607 (MH.sup.+) 110 H H
H --NHCO-- 205 M.P: 162-165.degree. C. MS (m/z): 591 ([M -
H].sup.-) 111 H CH.sub.3 H --NHCO-- 206 gum MS (m/z): 559, 561
(MH.sup.+) 112 H H H --NHCO-- 207 M.P.: 156-158.degree. C. MS
(m/z): 543 ([M - H].sup.-) 113 H CH.sub.3 H --NHCO-- 208 gum MS
(m/z): 549 (MH.sup.+) 114 H H H --NHCO-- 209 M.P.: 155-157.degree.
C. MS (m/z): 533 ([M - H].sup.-) 115 H CH.sub.3 H --NHCO-- 210 gum
MS (m/z): 557 (MH.sup.+) 116 H H H --NHCO-- 211 M.P.:
162-165.degree. C. MS (m/z): 541 ([M - H].sup.-) 117 H CH.sub.3 H
--NHCO-- 212 gum MS (m/z): 531 (MH.sup.+) 118 H H H --NHCO-- 213
M.P.: 235-237.degree. C. MS (m/z): 517 ([M - H].sup.-) 119 H
CH.sub.3 H --NHCO-- 214 gum MS (m/z): 515 (MH.sup.+) 120 H H H
--NHCO-- 215 M.P.: 143-145.degree. C. MS (m/z): 499 ([M - H].sup.-]
121 H H H --NHCO-- PhCH.sub.2-- M.P.: 240-242.degree. C. MS (m/z):
479 ([M - H].sup.-) 122 H H H --NHCO-- 216 M.P.: 212-214.degree. C.
MS (m/z): 563 ([M - H].sup.-) 123 H CH.sub.3 H --NHCO-- 217 gum MS
(m/z): 531 (MH.sup.+) 124 H H H --NHCO-- 218 M.P.: 141-143.degree.
C. MS (m/z): 515 ([M - H].sup.-) 125 H CH.sub.3 H --NHCO-- 219 gum
MS (m/z): 515 (MH.sup.+) 126 H H H --NHCO-- 220 M.P.:
145-147.degree. C. MS (m/z): 499 ([M - H].sup.-) 127 H CH.sub.3 H
--NHCO-- 221 gum MS (m/z): 515 (MH.sup.+) 128 H H H --NHCO-- 222
M.P.: 185-188.degree. C. MS (m/z): 499 ([M - H].sup.-) 129 t-Bu
CH.sub.3 H --NHCO-- 223 gum MS (m/z): 553 (MH.sup.+) 130 H H H
--NHCO-- 224 M.P.: 158-161.degree. C. MS (m/z): 455 ([M - H].sup.-)
131 t-Bu CH.sub.3 H --NHCO-- 225 gum MS (m/z): 527 (MH.sup.+) 132 H
H H --NHCO-- 226 M.P.: 117-120.degree. C. MS (m/z): 483 ([M -
H].sup.-) 133 H CH.sub.3 H --NHCO-- i-Bu gum MS (m/z): 461
(MH.sup.+) 134 H H H --NHCO-- i-Bu M.P.: 146- 148.degree. C. MS
(m/z): 445 ([M - H].sup.-) 135 H CH.sub.3 H --NHCO-- 227 gum MS
(m/z): 569 (MH.sup.+) 136 H H H --NHCO-- 228 M.P.: 160-163.degree.
C. MS (m/z): 553 ([M - H].sup.-) 137 H CH.sub.3 H --NHCO-- 229 gum
MS (m/z): 495 (MH.sup.+) 138 H CH.sub.3 H --NHCO-- 230 gum MS
(m/z): 569 (MH.sup.+) 139 H H H --NHCO-- 231 M.P.: 166-169.degree.
C. 140 H CH.sub.3 H --NHCO-- 232 gum MS (m/z): 599 (MH.sup.+) 141 H
H H --NHCO-- i-BuO M.P.: 125- 128.degree. C. MS (m/z): 461 ([M -
H].sup.-) 142 H H H --NHCO-- 233 M.P.: 152-155.degree. C. MS (m/z):
479 ([M - H].sup.-) 143 H H H --NHCO-- 234 M.P.: 148-150.degree. C.
MS (m/z): 481 ([M - H].sup.-) 144 H H H --NHCO-- 235 M.P.:
195-198.degree. C. MS (m/z): 480 ([M - H].sup.-) 145 H CH.sub.3 H
--NHCO-- 236 gum MS (m/z): 536 (MH.sup.+) 146 H H H --NHCO 237
M.P.: 208-211.degree. C. MS (m/z): 520 ([M - H].sup.-) 147 H
CH.sub.3 H --NHSO.sub.2-- 238 gum MS (m/z): 585 (MH.sup.+) 148 H H
H --NHSO.sub.2-- 239 M.P.: 224-226.degree. C. MS (m/z): 569 ([M -
H].sup.-) 149 H CH.sub.3 H --NHSO.sub.2-- 240 gum MS (m/z): 531
(MH.sup.+) 150 H H H --NHSO.sub.2-- 241 M.P.: 220-223.degree. C. MS
(m/z): 515 ([M - H].sup.-) 151 H CH.sub.3 H --NHCH.sub.2-- 242 gum
MS (m/z): 535 (MH.sup.+) 152 H H H --NHCH.sub.2-- M.P.:
100-103.degree. C. MS (m/z): 519 ([M - H].sup.-) 153 H H H --CONH--
243 MS (m/z): 533 ([M - H].sup.-)
Example 154
(1S-cis)-N-[[3-(Aminocarbonyl)-2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,-
6-dichlorophenyl)methyl]-L-tyrosine Methyl Ester
[0578] 244
[0579] BOP Reagent (674 mg) was added to a mixture of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichl-
orophenyl)methyl]-L-tyrosine methyl ester (743 mg) and aq.
NH.sub.4OH (0.5 ml) in THF (10 ml). The mixture was stirred for 24
hr. and sat. LiCl (15 ml) was added. The resulting mixture was
extracted with AcOEt and the extract was dried over
Na.sub.2SO.sub.4. The solvent was removed in vacuo and the residue
was purified by column chromatography on silica gel (eluent; Hexane
90% AcOEt/Hexane) to give (1S-cis)-N-[[3-(Aminocarbonyl)--
2,2,3-trimethylcyclopentyl]carbonyl]O-[(2,6-dichlorophenyl)methyl]-L-tyros-
ine methyl ester (533 mg). MS (m/z): 535 (MH.sup.+),
Example 155
(1S-cis)-N-[[3-(Aminocarbonyl)-2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,-
6-dichlorophenyl)methyl]-L-tyrosine
[0580] 245
[0581] A solution of LiOH (119 mg) in H.sub.2O (5 ml) was added to
a mixture of
(1S-cis)-N-[[3-(Aminocarbonyl)-2,2,3-trimethylcyclopentyl]carb-
onyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine methyl ester (533
mg) in THF (5 ml)/MeOH (3 ml). The mixture was stirred for 3 hr. at
room temperature, acidified with 1N HCl (20 ml), and extracted with
AcOEt. The extract was dried over Na.sub.2SO.sub.4 and evaporated
in vacuo to give
(1S-cis)-N-[[3-(Aminocarbonyl)-2,2,3-tri-methylcyclopentyl]carbonyl]-O-[(-
2,6-dichlorophenyl)-methyl]-L-tyrosine (466 mg), mp 102-104.degree.
C., MS (m/z): 519 ([M-H].sup.-).
[0582] Examples 156-166 were prepared in a similar manner, as
described in Examples 154 and 155 and are shown in Table 5.
6TABLE 5 246 Examples 156 through 166: Ex. physicochemical No.
R.sup.19 R.sup.20 R.sup.0 R.sup.6 property 156 247 H H 248 M.P.:
85-87.degree. C. MS (m/z): 533 ([M - H].sup.-) 157 Et H CH.sub.3
249 gum MS (m/z): 563 (MH.sup.+) 158 Et H H 250 MS (m/z): 550
(MH.sup.+) 159 Me H CH.sub.3 251 gum; .sup.1H NMR (300 MHz,
CD.sub.3OD), .delta. 7.3-7.4(3H), 7.14(2H), 6.95(2H), 5.22(2H),
4.71(1H), 3.69(3H), 3.12(1H), 3.00(1H), 2.39(3H), 2.3-2.4(1H),
2.0-2.1(1H), 1.7-1.8(1H), 1.4-1.5(1H), 1.27(3H), 1.18(3H),
0.73(3H). 160 Me H H 252 M.P.: 80-83.degree. C. MS (m/z): 533 ([M -
H].sup.-) 161 Me Me H 253 .sup.1H NMR(300 MHz, CDCl.sub.3), .delta.
7.3-7.4(3H), 7.16(2H), 7.00(2H), 5.25(2H), 4.75(1H), 3.66(3H),
3.22(1H), 3.13(1H), 2.5-2.7(1H), 2.1-2.2(1H), 2.05(3H), 2.03(3H),
1.7-1.8(1H), 1.4-1.6(1H), 1.20(3H), 1.19(3H), 0.73(2H). 162 MeO H
CH.sub.3 254 gum MS (m/z): 565 (MH.sup.+) 163 MeO H H 255 M.P.:
70-72.degree. C. MS (m/z): 551 ([M - H].sup.-) 164 Me H H 256 MS
(m/z): 509 (MH.sup.+) 165 Et H H 257 MS (m/z): 523 (MH.sup.+) 166 H
H H 258 M.P.: 196-198.degree. C. MS (m/z): 532 ([M - H].sup.-)
Example 167
(1R-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-1,2,2-trimethylcyclopentyl]c-
arbonyl]-O-(phenylmethyl)-L-tyrosine methyl ester
[0583] 259
[0584] Example 167 was prepared from O-benzyl-L-tyrosine methyl
ester hydrochloride and
(1R,3S)-3-(tert-butoxycarbonyl)-1,2,2-trimethylcyclopen-
tanecarboxylic acid in a similar manner as described in Example 52.
Physicochemical property: gum, MS (m/z): 524 (MH.sup.+).
Example 168
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-C-(phenylmethy-
l)-L-tyrosine Methyl Ester (C.sub.27H.sub.33NO.sub.6)
[0585] 260
[0586] Example 168 was prepared in a similar manner as described in
Example 53. Physicochemical property: gum; MS (m/z): 468
(MH.sup.+); MP 191-192.degree. C. (d).
Example 169
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-O-(phenylmethy-
l)-L-tyrosine (C.sub.26H.sub.31NO.sub.6)
[0587] 261
[0588] Example 169 was prepared in a similar manner as described in
Example 54, MS (m/z): 454 (MH.sup.+).
Example 170
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[N-acetyl-N-
-(2,6-dichlorophenyl)methyl]amino]-L-phenylalanine Methyl Ester
[0589] 262
[0590]
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclop-
entyl]carbonyl]-4-[N-[(2,6-dichlorophenyl)methyl]amino]-L-phenylalanine
methyl ester (0.29 g), which was derived from
4-[[N-(2,6-dichlorophenyl)m- ethyl]amino]-L-phenylalanine methyl
ester in a similar manner as described in Example 52, was dissolved
in pyridine (3 ml) and Ac.sub.2O (2.5 ml) was added. The mixture
was stirred overnight, evaporated in vacuo, and extracted with
AcOEt. The extract was washed with aq. HCl, brine, sat.
NaHCO.sub.3, brine, and dried over Na.sub.2SO.sub.4. The solvent
was removed in vacuo to give
(1S-cis)-N-[[3-[(1,1-dimethylethoxy)carbonyl]-2,-
2,3-trimethylcyclopentyl]carbonyl]-4-[[N-acetyl-N-[(2,6-dichlorophenyl)met-
hyl]amino]-L-phenylalanin methyl ester (0.24 g). The obtained
diester was treated in a similar manner as described in Examples 53
and 54 to give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[N-acetyl--
N-[(2,6-dichlorophenyl)methyl]amino]-L-phenylalanine methyl ester
as a colorless solid, mp 241-244.degree. C., MS (m/z): 561
([M-H].sup.-).
Example 171
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)methyl]-O-[(2,6-dichloro-
phenyl)methyl]-L-tyrosine Methyl Ester
(C.sub.17H.sub.33Cl.sub.2NO.sub.5)
[0591] 263
[0592] NaCNBH.sub.3 (104 mg) was added to a mixture of
O-[(2,6-dichlorophenyl)methyl]-L-tyrosine methyl ester (490 mg),
(1R,cis)-3-formyl-1,2,2-trimethylcyclopentanecarboxylic acid,
(1R,5S)-4-hydroxy-1,8,8-trimethyl-3-oxabicyclo[3,2,1]-octane-2-one
(153 mg), AcOH (0.5 ml), and MeOH (25 ml) under argon. The mixture
was stirred for 72 hr. at room temperature. The solvent was removed
in vacuo and 10% HCl (20 ml) was added. The resulting mixture was
stirred for 2 hr. and extracted with AcOEt. The extract was dried
over Na.sub.2SO.sub.4 and evaporated in vacuo. The residue was
purified by column chromatography on silica gel (eluent; 10%
AcOEt/Hexane) to give (1S-cis)-N-[(3-Carboxy-2,2,-
3-trimethylcyclopentyl)methyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
methyl ester (75 mg), MS (m/z) 522 (MH.sup.+)
Example 172
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)methyl]-O-[(2,6-dichloro-
phenyl)methyl]-L-tyrosine (C.sub.26H.sub.31Cl.sub.2NO.sub.5)
[0593] 264
[0594] A solution of LiOH (33 mg) in H.sub.2O (2 ml) was added to a
mixture of
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)methyl]-O-[(-
2,6-dichlorophenyl)methyl]-L-tyrosine methyl ester
(C.sub.27H.sub.33Cl.sub- .2NO.sub.5) (71 mg) in THF (2 ml). The
mixture was stirred for 5 hr. at room temperature, neutralized with
1N HCl, and extracted with AcOEt. The extract was dried over
Na.sub.2SO.sub.4 and evaporated in vacuo to give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)methyl]-O-[(2,6-dichlor-
ophenyl)methyl]-L-tyrosine (40 mg), mp 135-138.degree. C., MS
(m/z): 508 (MH.sup.+).
Example 173
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-N-methyl-O-(ph-
enylmethyl)-L-tyrosine (C.sub.27H.sub.33NO.sub.6)
[0595] 265
[0596]
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-(phen-
ylmethyl)-L-tyrosine (C.sub.26H.sub.31NO.sub.6) (52 mg) was added
to a suspension of NaH (20.5 mg, 60% in oil) in DMF (5 ml) and the
mixture was stirred for 10 min at room temperature. After addition
of MeI (43 ml), the mixture was stirred for 14 hr. and quenched
with H.sub.2O. The resulting mixture was extracted with ether. The
extract was evaporated in vacuo and the residue was subjected to
saponification using aq. LiOH in THF in a similar manner as
described in Example 54. The saponification was carried out for 2
hr. and the mixture was acidified with 1N HCl (10 ml) and extracted
with AcOEt. The extract was dried over Na.sub.2SO.sub.4, evaporated
in vacuo, and the residue was purified by column chromatography on
silica gel (eluent; 10% MeOH/CH.sub.2Cl.sub.2) to give
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-N-met-
hyl-O-(phenylmethyl)-L-tyrosine (40 mg), MS (m/z): 466 ([M-H])
Example 174
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlo-
rophenyl)methyl]-N-methyl-L-tyrosine
(C.sub.27H.sub.31Cl.sub.2NO.sub.6)
[0597] 266
[0598] Example 174 was prepared from
N-(tert-butoxycarbonyl)-O-[(2,6-dichl-
orophenyl)methyl]-N-methyl-L-tyrosine and
(1S,3R)-3-(tert-butoxycarbonyl)--
2,2,3-trimethylcyclopentanecarboxylic acid in a similar manner as
described in Example 52, 53 and 54, mp 108-110.degree. C., MS
(m/z): 536 (MH.sup.+).
Example 175
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlo-
rophenyl)methyl]-L-tyrosinamide
(C.sub.26H.sub.30Cl.sub.2N.sub.2O.sub.5)
[0599] 267
[0600] NaCN (10 mg) was added to a solution of
(1S-cis)-N-[(3-Carboxy-2,2,-
3-trimethylcyclopentyl)carbonyl]-O-[2,6-dichlorophenyl)methyl]-L-tyrosine
methyl ester (306 mg) in MeOH (10 ml) in a thick walled tube. 15-20
ml of NH.sub.3 was condensed into the mixture at -78.degree. C. The
tube was sealed and warmed to room temperature. After 48 hr.
stirring, the mixture was cooled to -78.degree. C. and the sealed
tube was opened. The mixture was warmed to room temperature and
excess NH.sub.3 was removed by bubbling N.sub.2. The solvent was
removed in vacuo and the residue was purified by column
chromatography on silica gel (eluent; AcOEt <10% MeOH/AcOEt) to
give (1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)car-
bonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosinamide (258 mg), mp
120-122.degree. C., MS (m/z): 519 ([M-H]-).
Example 176
[1S-[1.alpha.(R*),3.alpha.]-4-(2,6-Dichlorobenzoyl)-.alpha.-[[[3-[(1,1-dim-
ethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]amino]-.gamma.-o-
xo-1-piperazinebutanoic Acid Methyl Ester
[0601] 268
[0602] The synthesis of Example 176 is taught by Scheme 17.
Example 177
[1S[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopenty-
l)carbonyl]amino]-4-(2,6-dichiorobenzoyl)-.gamma.-oxo-1-piperazinebutanoic
Acid
[0603] 269
[0604] The synthesis of Example 177 is taught by Scheme 17.
Example 178
[1S-[1.alpha.((R*),3.alpha.]]-4-(2,6-Dichlorobenzoyl)-.beta.-[[[3-[(1,1-di-
methylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]amino]-.gamma.--
oxo-1-piperazinebutanoic Acid Methyl Ester
[0605] 270
[0606] The synthesis of Example 178 is taught by Scheme 18.
Example 179
[1S-[1.alpha.,3.alpha.(R*)]-N-[[3-[[(5-Amino-1-carboxypentyl)amino]carbony-
l]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzyl)amino]-L-ph-
enylalanine
[0607] 271
[0608] (1) To a solution of
N-(tert-butoxycarbonyl)-4-(2,6-dichlorobenzami- do)-L-phenylalanine
(9.25 g) in DMF (80-100 ml) was added Merrifield resin (10.0 g,
10.0 meq/g, Novabiochem) and anhydrous potassium fluoride (1.57 g).
The reaction mixture was stirred for 1 day at 80.degree. C. and the
resulting resin bound amino acid derivative was collected by
filtration, washed thoroughly with DMF, 50% aqueous DMF,
CH.sub.3OH, CH.sub.2Cl.sub.2, CH.sub.3OH, and then dried in vacuo
to give the resin bound
N-(tert-butoxycarbonyl)-4-(2,6-dichlorobenzamido)-L-phenylalanine
(0.53 meq/g). Substitution of the amino acid derivative onto the
resin was estimated using the picric acid method.
[0609] (2) To the obtained resin (1.0 g, 0.53 meq/g) was added 50%
TFA/CH.sub.2Cl.sub.2 (10-15 ml) and the mixture was stirred for 30
min. The resin was collected by filtration, washed with
CH.sub.2Cl.sub.2, CH.sub.3OH, and CH.sub.2Cl.sub.2. To the resin
bound 4-(2,6-dichlorobenzamido)-L-phenylalanine was added a mixture
of
(1S,3R)-3-(tert-butoxycarbonyl)-2,2,3-trimethylcyclopentanecarboxylic
acid (408 mg), 0.5M HBTU-HOBT (3.3 ml) in DMF, DIEA (0.694 ml), DMF
(10-15 ml) and the mixture was vortexed for 2 hrs. at room
temperature. The resin was collected by filtration, washed with
DMF, CH.sub.2Cl.sub.2, CH.sub.3OH, CH.sub.2Cl.sub.2 to give a resin
bound
(JS-cis)-N-[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]-
carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine (0.990 g).
To the mixture
[0610] (3) To the resin (0.390 g, 0.21 meq/g) was added 50%
TFA/CH.sub.2Cl.sub.2 (4-5 ml) and the mixture was stirred for 2
hrs. The resin was collected by filtration, washed with
CH.sub.2Cl.sub.21 CH.sub.3OH, and CH.sub.2Cl.sub.2. To the resin
bound
(1S-cis)-N-[[3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichl-
orobenzoyl)amino]-L-phenylalanine was added a mixture of
N.sup.6-(tert-butoxycarbonyl)-L-lysine trert-butyl ester (0.210 g),
0.5M HBTU-HOBT (1.5 ml) in DMF, DIEA (0.270 ml) and the mixture was
vortexed for 2 hrs. The resin was collected by filtration, washed
with DMF, CH.sub.2Cl.sub.2, CH.sub.3OH, and CH.sub.2Cl.sub.2. To
the obtained resin was added 50% TFA/CH.sub.2Cl.sub.2 (4-5 ml) and
the mixture was stirred for 30 min. The resin was collected by
filtration, washed with CH.sub.2Cl.sub.2, CH.sub.3OH, and
CH.sub.2Cl.sub.2. To the resin was added THF (3 ml), CH.sub.3OH
(0.9 ml) and 2N LiOH (0.3 ml). The mixture wasd stirred for 15 mins
and filtered to a test tube (13.times.100 mm). The resin was washed
with THF/5% CH.sub.3OH and the combined filtrate was evaporated.
The residue was dilued with H.sub.2O (1 ml) and acidified with 1N
HCl. The precipitate was centrifuged. The supernatant was decanted,
and the pellet was washed with H.sub.2O. The residue was
lyophilized to give a colorless solid (100 mg).
[0611] (4) The solid (80 mg) was purified by a Water Delta Prep
3000 system (Waters, Milford, Mass.) equipped with a reversed phase
silica C18 column (4.7 cm.times.30.0 cm), using a linear gradient
of increasing acetonitrile in an aqueous solution of
triethylammonium phosphate (TEAP) (prepared by diluting phosphoric
acid (25 ml) and triethylamine (50 ml) to 6000 ml of deionized
water, pH -5.5). The fractions containing desired compound was
desalted with 0.1% acetic acid using the above system The collected
fractions were lyophilized to give [1S-[1.alpha.,3.alpha.(R*)]--
N-[[3-[[(5-Amino-1-carboxypentyl)amino]carbonyl]-2,2,3-trimethylcyclopenty-
l]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine (Example
179) as a colorless solid (10 mg), ESMS: 661 (M-H]).sup.-.
Example 180
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethylcyclop-
entyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.28H.sub.32Cl.sub.2N.sub.4O.sub.6)
[0612] 272
[0613] The synthesis of Example 180 is taught by Scheme 8, 8-G:
wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amin- o]-phenyl,
Stereochemistry=(1S-cis)-L. Accordingly, the synthesis of
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-4-[(2,6-dichlorobenzoyl) amino]-L-phenylalanine
(C.sub.28H.sub.32Cl.sub.2N.sub.4O.sub.6) is as follows.
[0614] To 8-F-1 (8F: R.sup.8-1=H, R.sup.8-2=HE, R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorobenzoyl)amino]-phenyl,
Stereochemistry=(1S-cis)-L) (1.05 g, 1.7 mmol), dissolved in
methanol (30 mL), is added a solution of LiOH.sub.2H.sub.2O (0.32
g, 7.65 mmol) in water (10 mL), dropwise over 15 minutes. The
mixture stirs for 18 hours at room temperature and the pH is then
adjusted to ca. 7 by the careful addition of 1N aq. HCl. The
majority of the methanol, is removed in vacuo and the pH of the
resulting solution is adjusted to ca. 2 with 1N aq. HCl. The
resulting flocculent white precipitate is isolated by filtration
and dried. The solid is crushed and washed with water (2.times.10
mL) and dried in vacuo at 50`C` to give 0.97 g (97%) of Example 180
(8-G: R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.6=4-[(2,6-dichlorobenzoyl)amin- o]-phenyl) as a white,
powdery solid.
[0615] MP: 203-205.degree. C.; .sup.1H NMR (300 MHz, DMSO-d.sub.6):
.delta. 12.51 (brs, 1H), 10.70 (s, 1H), 7.75 (m, 1H), 7.45-7.57
(3H), 7.33 (m, 1H), 7.20 (m, 2H), 7.11 (brs, 1H), 6.92 (brs, 1H),
4.43 (m, 1H), 3.63 (m, 1H), 3.47 (m, 2H), 3.30 (s, 2H), 3.01 (m,
1H), 2.84 (m, 1H), 2.31 (m, 1H), 1.87 (m, 1H), 1.55 (m, 1H), 1.31
(m, 1H), 1.17 (s, 3H), 1.08 (s, 3H), 0.59 (s, 3H); IR (Mull): 3511,
3325, 3128, 3082, 2868, 1722, 1697, 1664, 1614, 1555, 1537, 1417,
1337, 1246, 799; MS (FAB) m/z (rel. intensity) 591 (M+H, base), 517
(32), 335 (26), 239 (32), 173 (39), 109 (63), 57 (80); HRMS (FAB)
calcd for C.sub.28H.sub.32Cl.sub.2N.sub.4O.- sub.6 +H.sup.+
591.1777, found 591.1747.
Example 181
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcyclopentyl-
]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.28H.sub.31 .sub.1C.sub.2N.sub.3O.sub.7)
[0616] 273
[0617] The synthesis of Example 181 is taught by scheme 7, 7-G:
wherein R.sup.7-1=H, R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorobenzoy- l)amino]phenyl and
Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcyclopenty-
l]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7) is synthesized as
follows:
[0618]
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcycl-
opentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
methyl ester (0.7 g, 1.15 mmol) is dissolved in methanol (12 mL).
To this is added a mixture of LiOH.H.sub.2O (0.243 g, 5.8 mmol),
aqueous H.sub.2O.sub.2 (30%, 2 mL), and H.sub.2O (2 mL). After
overnight stirring, the reaction mixture is diluted with water (50
mL), and evaporated (room temperature, in vacuo/N.sub.2 flow) until
the methanol is gone. The aqueous solution is then transferred to a
separatory funnel and shaken with diethyl ether (2.times.20 mL).
The aqueous layer is then evaporated, to remove residual diethyl
ether, and cooled in an ice water bath. The stirred solution is
then brought to pH 3-4 using aqueous HCl (1N). The resultant
precipitate is isolated by suction filtration (with water washes)
to give the target compound as a white solid (0.4 g, 58% yield)
[0619] .sup.1HNMR: (300 MHz, DMSO-d): .delta. 12.45 (br.s, 1H),
10.6 (s, 1H), 7.74 (m, 2H), 7.57-7.44 (m, 3H), 7.20 (m, 2H),
4.48-4.40 (m, 1H), 3.65 (m, 2H), 2.94 (m, 2H), 2.64 (m, 1H), 2.35
(m, 1H), 1.90 (m, 1H), 1.58 (m, 1H), 1,29 (m, 1H), 1.18 (s, 3H),
1.08 (s, 3H), 0.60 (s, 3H); IR (mull) 3124, 3088, 3078, 1738, 1666,
1628, 1612, 1588, 1563, 1552, 1521, 1429, 1334, 1197, 1170
cm.sup.-1; MS (FAB) m/z (rel. intensity) 592 (M+H, 99), 595 (20),
594 (69), 593 (41), 592 (99), 519 (25), 517 (38), 240 (55), 175
(23), 173 (33), 109 (64); HRMS (FAB) m/z calcd for
C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7+H.sup.+ 592.1617, found
592.1606; Anal. Calcd for C.sub.28H.sub.31Cl.sub.2N.sub.3O.sub.7:
C, 56.76; H, 5.27; N, 7.09; Found: C, 54.92; H, 5.41; N, 6.91; KF
Water: 3.05F H.sub.2O.
Example 182
[1S-[1.alpha.,3.alpha.(E)-N-[[3-(2-Carboxyethenyl)-2,2,3-trimethylcyclopen-
tyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
Disodium Salt (C.sub.28H.sub.28Cl.sub.2N.sub.2Na.sub.2O.sub.6)
[0620] 274
[0621] The synthesis of Example 182 is taught by Scheme 11, 11-G:
wherein R.sup.4=H, R.sup.5=CO.sub.2H
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-pheny- l and
Stereochemistry=[1S-[1.alpha.,3.alpha.(E)]]-L. Accordingly,
[1S-[1.alpha.,3.alpha.(E)]]-N-[[3-(2-Carboxyethenyl)-2,2,3-trimethylcyclo-
pentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
disodium salt (C.sub.28H.sub.28Cl.sub.2Na.sub.2N.sub.2O.sub.6) of
11-G is synthesized as follows:
[0622] To a solution of 11-F (R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlorobenzoyl)amino]-phenyl,
Stereochemistry=[1S-[1.alp- ha.,3.alpha.(E)]]-L) (0.45 g, 0.78
mmol) in methanol (5 mL) in a flask cooled in an ice water bath is
added a solution of LiOH.H.sub.2O (0.127 g, 3 mmol) in H.sub.2O (5
mL). After two days, the mixture is diluted with water (50 mL),
evaporated in vacuo until the methanol is gone and then cooled to
1.degree. C. and brought to pH 2 using 1N HCl. The resultant white
precipitate is isolated by suction filtration to give a white solid
which is stirred with saturated aqueous NaHCO.sub.3 (2 mL) and then
transferred to a C-18 reversed phase HPLC column and eluted with a
gradient from 0.01% aq NaHCO.sub.3 to 100% acetonitrile/0.01% aq
NaHCO.sub.3. Evaporation in vacuo to give Example 182 as a white
solid (0.25 g, 51% yield).
[0623] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 7.51-7.42 (m,
5H), 7.06 (m, 2H), 6.39 (m, 1H), 5.49 (m, 1H), 4.10 (m, 1H) 2.99
(m, 1H), 2.86 (m, 1H), 2.56 (m, 1H), 1.85 (m, 2H), 1.61 (m, 1H),
1.29 (m, 1H), 0.90 (s, 3H), 0.85 (s, 3H), 0.51 (s, 3H); IR (mull)
3393, 3257, 3124, 3035, 1654, 1604, 1562, 1544, 1515, 1431, 1398,
1325, 799, 773, 722 cm.sup.-1; MS (FAB) m/z (rel. intensity) 605
(M+H, 44), 629 (9), 627 (14), 608 (8), 607 (30), 606 (14), 605
(44), 585 (14), 583 (21)l, 73 (45), 23 (99); KF Water:
7.09%H.sub.2O.
Example 183
(1S-cis)-N-[[3-[(Carboxymethoxy)methyl]-2,2,3-trimethylcyclopentyl]carbony-
l]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.28H.sub.31Cl.sub.- 2N.sub.3O.sub.7)
[0624] 275
[0625] The synthesis of Example 183 is taught by Scheme 9 and by
the narrative accompanying Scheme 9.
Example 184
(1S-cis)-N-[(3-Cyano-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichloro-
benzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.27Cl.sub.2N.sub.3O.sub.4)
[0626] 276
[0627] The synthesis of Example 184 is taught by Scheme 12 under
the heading Preparation of Example 184.
Example 185
(1S-cis)-N-[[3-(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.28H.sub.32Cl.sub- .2N.sub.2O.sub.7)
[0628] 277
[0629] The synthesis of Example 185 is taught by scheme 7, 7-G:
wherein R.sup.7-1=H, R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-(2,6-Dichlorophenyl)- methoxy]-phenyl and
Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis)-N-[[3-[[(Carboxymethyl)amino]carbonyl]-2,2,3-trimethylcyclopenty-
l]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.28H.sub.32Cl.sub.2N.sub.2O.sub.7) is prepared from 7-G-2
(7-G: R.sup.7-1=H, R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-Dichlor- ophenyl)methoxy]phenyl,
Stereochemistry=(1S-cis)-L as like as Example 181.
[0630] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 708-6.9 (m, 9H),
5.18 (s, 2H), 4.42 (m, 1H), 3.8-3.6 (m, 2H) 3.02-2.82 (m, 2H), 2.65
(m, 1H), 2.38 (m, 1H), 1.91 (m, 1H), 1.58 (m, 1H), 1.30 (m, 1H),
1.19 (s, 3H), 1.10 (s, 3H), 0.61 (s, 3H); IR (mull) 3409, 1733,
1645, 1612, 1585, 1564, 1511, 1439, 1297, 1239, 1197, 1179, 1018,
786, 770 cm.sup.-1; MS (FAB) m/z (rel. intensity) 579 (M+H, 99),
582 (22), 581 (67), 580 (44), 579 (99), 578 (21), 240 (34), 161
(21), 159 (34), 109 (46), 91 (37); HRMS (FAB) calcd for
C.sub.28H.sub.32Cl.sub.2N.sub.2O+H.sup.+ 579.1664, found
579.1667.
Example 186
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)
carbonyl]-O-[(2,6-dichl- orophenyl)methyl]-L-tyrosine
(C.sub.26H.sub.29Cl.sub.2NO.sub.6)
[0631] 278
[0632] Compound III-a (where R.sup.4=H, R.sup.5a=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl, n=1, Stereochemistry
S) and (1R)-camphoric anhydride were heated together in
diisopropylethyl amine. The crude mixture was concentrated in vacuo
to yield crude I-b (where R.sup.1=H, R.sup.3=CH.sub.3, R.sup.4=H,
R.sup.5a=CO.sub.2CH.sub.3,
R.sup.6=4-(2,6-dichlorophenyl)methoxy]phenyl, n=1,
Stereochemistry=(1R-cis)-L). I-b was hydrolyzed with LiOH to yield
Example 186 (1-a; where R.sup.1=H, R.sup.3=CH.sub.3, R.sup.4=H,
R.sup.5=CO.sub.2H, and
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl, n=1, Stereochemistry
(1R-cis)-L): .sup.13C NMR .delta. 175.9, 175.4, 173.0, 158.6,
137.3, 133.0, 132.0, 131.3, 130.9, 129.7, 115.5, 65.9, 56,5, 54.2,
53.0, 47,0, 36.8, 33.1, 23.2, 22.8, 22.0, 21.0.
Example 187
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethylcyclop-
entyl]carbonyl-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.28H.sub.33Cl.sub.2N.sub.3O.sub.6)
[0633] 279
[0634] The synthesis of Example 187 is taught by Scheme 8, 8-G:
wherein R.sup.8-1=H, R.sup.8-2=H, R.sup.4=H,
R.sup.6=4-[(2,6-2,6-Dichlorophenyl)m- ethoxy]-phenyl
Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethylcyclo-
pentyl]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.28H.sub.33Cl.sub.2N.sub.3O.sub.6) is prepared from 8-F-2
(R.sup.8-1=H, R.sup.8-2H, R.sup.4=H, R.sup.5=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]-phenyl,
Stereochemistry=(1S-cis)-- L) as taught by Scheme 8.
[0635] .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 7.74 (m, 2H),
7.55-7.40 (m, 4H,/, 7.15 (m, 3H), 6.94 (m, 3H), 5.16 (s, 2H), 4.41
(m, 1H), 3.75-3.48 (m, 2H), 3.1-2.8 (m, 211H), 2.63 (m, 1H), 2.33
(m, 1H), 1.87 (m, 1H), 1.54 (m, 1H), 1.32 (m, 1H), 1.17 (s, 3H),
1.08 (s, 3H), 0.58 (s, 3H); MS (FAB) m/z (rel. intensity) 578 (M+H,
99), 581 (30), 580 (72), 579 (57), 578 (99), 577 (19), 504 (17),
322 (18), 239 (35), 161 (29), 159 (34); HRMS (FAB) calcd for
C.sub.28H.sub.33Cl.sub.2N.sub.3O.sub.6 +H.sup.+ 578.1824, found
578.1836.
Example 188
[1S-[1.alpha.,3.alpha.(R*)
1-N-[[3-(1-Carboxyethyl)amino]carbonyl]-2,2,3-t-
rimethylcyclopentyl]carbonyl]-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.29H.sub.34Cl.sub.2N.sub.2O.sub.7)
[0636] 280
[0637] The synthesis for Example 188 is taught by scheme 7, 7-G:
wherein R.sup.7-1=CH.sub.3, R.sup.4=H, R.sup.5=CO.sub.2H,
R.sup.6=4-[(2,6-Dichlor- ophenyl)methoxy]-phenyl and
Stereochemistry [1S-[1.alpha.,3.alpha.(R*)]-L. Accordingly,
[1S-[1.alpha.,3.alpha.(R*)]]-N-[[3-[[(1-Carboxyethyl)amino]c-
arbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,6-dichlorophenyl)methy-
l]-L-tyrosine (C.sub.29H.sub.34Cl.sub.2N.sub.2O.sub.7) is prepared
from 7-G-3 as taught by Scheme 7, .sup.1H NMR (300 MHz,
DMSO-d.sub.6) .delta. 7.71 (m, 1H), 7.54-7.43 (m, 3H), 7.28 (m,
1H), 7.16 (m, 2H), 6.93 (m, 2H), 5.16 (s, 2H), 4.40 (m, 1H), 4.16
(m, 1H), 3.02-2.80 (m, 2H), 2.63 (m, 1H), 2.35 (m, 1H), 1.86 (m,
1H), 1.54 (m, 1H), 1.35-1.23 (m, 4H) 1.14 (s, 3H), 1.08 (s, 3H),
0.59 (s, 3H); IR (mull) 3427, 3031, 1731, 1645, 1612, 1585, 1565,
1512, 1439, 1297, 1239, 1230, 1197, 1179, 1017 cm.sup.-1; MS (FAB)
m/z (rel. intensity) 593 (M+H, 99), 596 (22), 595 (69), 594 (43),
593 (99), 592 (17), 504 (22), 254 (63), 161 (44), 159 (40), 109
(72)
Example 189
(1R-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlo-
rophenyl)methyl]-L-tyrosine (C.sub.26H.sub.29Cl.sub.2NO.sub.6)
[0638] 281
[0639] The ester III-a (R.sup.4=H, R.sup.5a=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl, n=1,
Stereochemistry=R) (391 mg, 1 mmol), THF (3 mL), and
diisopropylethyl amine (880 .mu.L, 5 mmol) were combined.
(1S)-Camphoric anhydride (33-A) (182 mg, 1 mmol) was added and the
reaction was heated at reflux for 18 h. The reaction mixture was
then cooled and concentrated in vacuo. The material was treated
with 1 N HCl (1 mL) and extracted with EtOAc (3.times.5 mL). The
organic portion was dried and concentrated in vacuo to yield 590 mg
of crude methyl ester I-a as a mixture of regioisomers and
diastereomers. Only characteristic and easily discernable protons
are listed for the major product
(1R-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-
-O-[(2,6-dichlorophenyl)methyl]-L-tyrosine methyl ester (1-a).
.sup.1H NMR (CDCl.sub.3) .delta. 0.75 (s, H), 1.00 (s, H), 1.19 (s,
H), 3.75 (s, H), 4.84-5.00 (m, H), 5.23 (s, H).
[0640] The crude methyl ester I-a (560 mg, 1 mmol) was combined
with LiOH.sub.2O (420 mg, 10 mmol) that was dissolved in 6 mL of
H.sub.2O. After 4.5 h of stirring on the rotovap, 1 N HCl (12 mL)
was added to the reaction mixture and a white precipitate was
formed. The mixture was-extracted with EtOAc (3.times.20 mL). The
organic portion was dried and concentrated in vacuo to afford 480
mg of crude di-acid Example 189. The material was purified by a
Cl.sub.8 reverse phase chromatography (on the Delta Prep employing
a Delta-PAK C.sub.18, 15 .mu.M particle size column that was
conditioned with CH.sub.3CN/H.sub.2O/TFA (40:60:0.1)). The material
was eluted with CH.sub.3CN/H.sub.2O (38:62) to yield 190 mg (36%
overall from anhydride) of Example 189:
[.alpha.].sub.D.about.4.5.de- gree. (c=0.6, EtOH),
[.alpha.].sub.D[2.8.degree. (c=0.8, EtOH); .sup.1H NMR
(DMSO-d.sub.6) .delta. 0.21 (s), 0.59 (s), 0.86 (s), 1.05-1.18 (m),
1.28-1.45 (m), 1.64-1.82 (m), 2.05-2.20 (m), 2.41 (m), 2.57 (m),
2.87 (m), 4.22-4.32 (m), 4.95 (s), 6.73 (m), 6.97 (m), 7.20-7.30
(m), 7.34 (m), 7.61 (m); .sup.13C NMR (DMSO-d.sub.6) .delta. 176.9
(s), 173.4 (s), 172.0 (s), 157.1 (s), 136.0 (s), 131.8 (s), 131.5
(d), 130.3 (s), 130.1 (d), 128.7 (d), 114.3 (d), 64.9 (t), 55.4
(s), 53.5 (d), 52.2 (d), 45.5 (s), 35.9 (t), 32.3 (t), 22.4 (t),
22.0 (q), 21.5 (q), 20,8 (q); IR (mineral oil mull) 3296, 2925,
1712, 1698, 1651, 1512, 1438, 1242, cm.sup.-1; MS for
C.sub.26H.sub.29Cl.sub.2NO.sub.6, m/Z (relative intensity) 523
(M.sup.+, 1), 521 (M.sup.+, 1), 503 (1), 477 (1), 324 (29), 322
(45), 267 (19), 265 (28), 161 (64), 159 (100). Anal. Calcd for
C.sub.26H.sub.29Cl.sub.2NO.sub.6: C, 59.78; H, 5.60; Cl, 13.57; N,
2.68. Found: C, 59.41; H, 5.55; Cl, 13.43; N, 2.52. Corrected for
1.14% H.sub.2O found by Karl Fisher analysis.
Example 190
[1S-[1.alpha.(R*),3.alpha.]]-N-[[3-Carboxy-2,2,3-trimethylcyclopentyl)carb-
onyl]-.alpha.-methoxymethyl-4-[(2,6-dichlorophenyl)methoxy]benzene-ethanam-
ine
[0641] 282
[0642] The synthesis of Example 190 is taught by Scheme 25.
Example 191
(1S-cis]-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-(hydroxyphen-
ylmethyl)-L-phenylalanine (C.sub.26H.sub.31NO.sub.6)
[0643] 283
[0644] The synthesis of Example 191 is taughtby scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=Me, R.sup.3=R.sup.4=H,
R.sup.5=--CO.sub.2H, R.sup.6=4-(hydroxyphenylmethyl)-phenyl,
X=--C(O)-- and Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis]-N-[(3-Carboxy-2,2,3-tri-
methylcyclopentyl)carbonyl]-4-(hydroxyphenylmethyl)-L-phenylalanine
(C.sub.26H.sub.31NO.sub.6) is synthesized as follows:
[0645] To a solution of Example 40 (0.59 g, 1.3 mmol) in absolute
EtOH under N.sub.2 at 0.degree. C. is added NaBH.sub.4 (100 mg, 2.7
mmol). The reaction mixture is stirred for 17 h at room temperature
diluted with H.sub.2O, and quenched with 1 N HCl. The solution is
treated with satd NH.sub.4Cl and adjusted to approximately pH 3
with 1 N HCl The mixture is extracted with EtOAc. The combined
extracts are dried, filtered and concentrated to give a colorless
oil (0.60 g). The product is purified by C18 reverse phase
chromatography (isocratic 35:65 CH.sub.3CN/H.sub.2O). The product
(0.29 g) is diluted with MeOH/H.sub.2O, frozen and lyophilized to
give Example 191 as a white powder: TLC R.sub.f=0.18 (650:350:1
CHCl.sub.3/acetone/HCO.sub.2H); HPLC t.sub.R=7.3 min (isocratic
650:350:1 CH.sub.3CN/H.sub.2O/TFA); [.alpha.].sub.D.sup.25+26.-
degree. (c 0.48, MeOH); IR (mull) 3327, 1709, 1653, 1514, 1242,
1207, 1118, 1017, 950, 700 cm.sup.31 1; .sup.1H NMR (CD.sub.3OD)
.delta. 7.37-7.14 (9H), 5.73 (1H), 4.90 (2H), 4.66 (1H), 3.20 (1H),
2.96 (1H), 2.70-2.62 (1H), 2.48 (1H), 1.92 (1H), 1.59 (1H), 1.40
(1H), 1.19 (3H), 1.18 (3H), 0.74 (3H); .sup.13C NMR (CD.sub.3OD)
.delta. 178.28, 173.84, 144.56, 142.97, 136.31, 128.84, 127.86,
126.79, 126.33, 126.31, 75.37, 56.00, 53.16, 46.19, 39.04, 36.69,
32.33, 22.43, 21.72, 21.04, 20.43; MS (FAB, HR) m/z 454.2234 (calcd
[M+H].sup.+454.2229); MS (FAB) m/z 493, 454, 476, 436, 237, 226,
208, 109; Anal. C, 65.54; H, 6.74; N, 3.12 (calcd C, 68.86; H,
6.89; N, 3.09.
Example 192
(1S-cis)-N-[[3-(hydroxymethyl)-2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,-
6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.26H.sub.31Cl.sub.2NO.sub.5)
[0646] 284
[0647] The synthesis of Example 192 is taught by Scheme 10 under
the heading Preparation of Example 192.
Example 193
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-O-(2,6-dichlor-
ophenyl)methyl]-D-tyrosine (C.sub.26H.sub.29Cl.sub.2NO.sub.6)
[0648] 285
[0649] MeOH (10 mL) was cooled to 0.degree. C. and acetyl chloride
(2.14 mL, 30 mmol) was added slowly. After 20 min the
t-BOC-D-tyrosine derivative III-a (where R.sup.4=H,
R.sup.5a=CO.sub.2CH.sub.3,
R.sup.6=4-[(2,6-dichlorophenyl)methoxy]phenyl, n=1,
Stereochemistry=R), (2.64 g, 6 mmol) was added. The reaction
mixture was stirred at room temperature for 2 h. The mixture was
concentrated under vacuum to afford a white solid. The white solid
was triturated with Et.sub.2O and filtered. The solid was returned
to the flask and was combined with THF (15 mL), diisopropylethyl
amine (4.4 mL, 25 mmol), and (1R)-camphoric anhydride (1.0 g, 5
mmol). The reaction mixture was heated at reflux for 18 h. The
mixture was then cooled and concentrated in vacuo. The material was
treated with 1 N HCl (40 mL) and extracted with EtOAc (3.times.20
mL). The organic portion was dried and concentrated in vacuo to
yield 3.30 g of crude methyl ester I-a as a mixture of regioisomers
and diastereomers.
[0650] The crude methyl ester I-a (2.79 g, 5 mmol) was combined
with LiOH.H.sub.2O (2.1 g, 50 mmol) that was dissolved in 30 mL of
H.sub.2O. After 4.0 h of stirring on the rotovap, 1 N HCl (60 mL)
was added to the reaction and a white precipitate formed. The
mixture was extracted with EtOAc (3.times.40 mL). The organic
portion was dried and concentrated in vacuo to afford 3.05 g of
crude di-acid. The material was chromatographed on 150 g of silica
gel eluting with CHCl.sub.2/EtOAc/HCO.sub.2H (50:50:0.1) to yield
pure (1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopent-
yl)carbonyl]-O-(2,6-dichlorophenyl)methyl]-D-tyrosine (Example
193). This material was dissolved in 20 mL of CH.sub.3CN/H.sub.2O
(1:1). The mixture was concentrated in vacuo until the liquid
appeared milky. The solution was frozen and lyophilized to afford
Example 193: .sup.1H NMR (DMSO-d.sub.6) .delta. 0.55 (H), 1.13 (H),
1.25 (H), 1.30-1.45 (1H), 1.68-1.86 (1H), 1.95-2.122 (1H),
2.25-2.41 (1H), 2.73, 2.95-3.20, 4.49-4.62, 5.23, 7.01, 7.25,
7.30-7.40, 7.45-7.70; .sup.13C NMR (DMSO-d.sub.6) .delta. 175.3
(s), 174.5 (s), 173.5 (s), 157.3 (s), 136.3 (s), 132.0 (d), 131.7
(s), 130.9 (s), 130.5 (d), 129.0 (d), 114.4 (d), 65.1 (t), 55.3
(s), 53.6 (d), 52.2 (d), 46.3 (s), 35.6 (t), 32.1 (t), 23.2 (q),
22.2 (t), 21.4 (q), 20.6 (q); IR (mineral oil mull) 3436, 2922,
1722, 1707, 1634, 1612, 1512, 1438, 1242, cm.sup.-1; MS for
C.sub.26H.sub.29Cl.sub.2NO.sub.6, m/z (relative intensity) 523
(M.sup.+, 0.4), 521 (M.sup.+, 0.5), 505 (2), 503 (2), 324 (33), 322
(50), 267 (21), 265 (32), 161 (69), 159 (100). Anal. Calcd for
C.sub.26H.sub.29Cl.sub.2NO- .sub.6: C, 59.78; H, 5.60; Cl, 13.57;
N, 268. Found: C, 59.52; H, 5.29; Cl, 13.60; N, 2.52. Corrected for
0.9% H.sub.2O found by Karl Fisher analysis.
Example 194
[1S[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopenty-
l)
carbonyl]amino]-6-chloro-5-[(2,6-dichlorophenyl)methoxy]-2-pyridineprop-
anoic Acid
[0651] 286
[0652] The synthesis of Example 194 is taught by Scheme 34.
Example 195
[1S-[1.alpha.(R*),3.alpha.]]-.beta.-[[(3-Carboxy-2,2,3-trimethylcyclopenty-
i)carbonyl]amino]-4-(2,6-dichlorobenzoyl)-.gamma.-oxo-1-piperazinebutanoic
Acid
[0653] 287
[0654] The synthesis of Example 195 is taught by Scheme 18.
Example 196
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-1-(phenylmethy-
l)-L-tryptophan (C.sub.28H.sub.32N.sub.2O.sub.5)
[0655] 288
[0656] Example 196 was synthesized as described Scheme 24.
Example 197
[1R-cis]-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-(hydroxyphen-
ylmethyl)-L-phenylalanine (C.sub.26H.sub.31NO.sub.6)
[0657] 289
[0658] The synthesis of Example 197 is taught by scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=R.sup.4=H, R.sup.3=Me,
R.sup.5=--CO.sub.2H, R.sup.6=4-(hydroxyphenylmethyl)-phenyl,
X=--C(O)-- and Stereochemistry=(1R-cis)-L. Accordingly,
[1R-cis]-N-[(3-Carboxy-1,2,2-tri-
methylcyclopentyl)carbonyl]-4-(hydroxyphenylmethyl)-L-phenylalanine
(C.sub.26H.sub.31NO.sub.6) is prepared from 32-B and (1R)-camphoric
anhydride by the procedures taught by Scheme 2 and by the synthesis
of Examples 40, 186, and 191: TLC silica gel R.sub.f=0.31
(900:100:1 CHCl.sub.3/MeOH/HCO.sub.2H); HPLC t.sub.R=5.6 min
(isocratic 650:350:1 CH.sub.3CN/H.sub.2O/TFA);
[.alpha.].sub.D.sup.25+24.degree. (c 0.38, MeOH); UV (MeOH)
.lambda..sub.max (.epsilon.) 223 (14200), 254 (425 sh), 258 (493),
263 (481), 268 (364 sh), 272 (257 sh); IR (mull) 3379, 1712, 1642,
1514, 1417, 1377, 1342, 1274, 1179, 1133, 1017, 700 cm.sup.-1;
.sup.1H NMR (CD.sub.3OD) .delta. 7.35-7.15 (m 9H), 5.73 (1H), 4.91
(3H), 4.72 (1H), 3.26 (1H), 2.99 (1H), 2.72 (1H), 2.44-2.30 (1H),
2.15-2.00 (1H), 1.82-1.67 (1H), 1.40-1.27 (1H), 1.05 (3H), 1.03
(3H), 0.54 (3H), 0.53 (3H); .sup.13C NMR (CD.sub.3OD) .delta.
176.29, 176.24, 173.56, 144.56, 143.17, 136.26, 136.23, 128.82,
127.88, 126.85, 126.39, 126.31, 75.33, 55.77, 53.34, 52.47, 46.20,
36.09, 32.03, 22.11, 21.98, 20.17, 20.07; MS (HR FAB) m/z 454.2230
(calcd [M+H].sup.+454.2229); MS (FAB) m/z 454, 436, 237, 226, 208,
109; H.sub.2O (Karl Fischer) 1.44W; Anal. C, 67.68; H, 6.98; N,
3.06 (calcd adjusted for H.sub.2O: C, 68.86; H, 6.89; N, 3.09).
Example 198
(1S-cis)-N-[[(3-methoxymethyl)-2,2,3-trimethylcyclopentyl]carbonyl]-O-[(2,-
6-dichlorophenyl)methyl]-L-tyrosine
(C.sub.27H.sub.33Cl.sub.2NO.sub.5)
[0659] 290
[0660] The synthesis of Example 198 is taught by Scheme 10 under
the heading Preparation of Example 198.
Example 199
(1R-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-O-[(2,6-dichlo-
rophenyl)methyl]-D-tyrosine (C.sub.26H.sub.29Cl.sub.2NO.sub.6)
[0661] 291
[0662] In the same manner as the synthesis of 189, 199 was
synthesized and purified (0.5 g, 49% overall yield):
[.alpha.].sub.D=-31.degree..
Example 200
[1R-[1.alpha.,3.alpha.(S*)]]-N-[[3-[[[1-Carboxy-2-[4-[(2,6-dichlorophenyl)-
methoxy]phenyl]ethyl]-amino]carbonyl]-1,2,2-trimethylcyclopentyl]carbonyl]-
-L-proline 1,1-dimethylethyl Ester, Monosodium Salt
(C.sub.35H.sub.43C.sub.2N.sub.2NaO.sub.7)
[0663] 292
[0664] The sythesis of Example 200 is taught by Scheme 21.
Example 201
[1S-[1.alpha.(R*),3.alpha.]]-Z-[[(3-Carboxy-2,2,3-trimethylcyclopentyl)car-
bonyl]amino]-4-[(2,6-dichlorophenyl)methoxy]-benzenebutanoic Acid
(C.sub.27H.sub.31Cl.sub.2NO.sub.6)
[0665] 293
[0666] The sythesis of Example 201 is taught by scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=Me, R.sup.3=R.sup.4=H,
R.sup.5=--CH.sub.2CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]-phen- yl-, n=1,
Stereochemistry=[1S-[1.alpha.(R*),3.alpha.]]. Accordingly,
[1S-[1.alpha.(R*),3.alpha.]]-.beta.-[[(3-Carboxy-2,2,3-trimethylcyclopent-
yl)carbonyl]amino]-4-[(2,6-dichlorophenyl)methoxy]-benzenebutanoic
acid (C.sub.27H.sub.31Cl.sub.2NO.sub.6) is prepared from 30-B and
15-D as taught by Scheme 2. TLC: R.sub.f=0.22 (50:50.0.2
hexanes/ELOAc/HCO.sub.2H- ); [.alpha.].sub.D+3 (MeOH); IR 3320,
3057, 3026, 2953, 2924, 2868, 2855, 1703, 1641, 1612, 1585, 1565,
1511, 1465, 1439, 1403, 1378, 1297, 1283, 1241, 1197, 1179, 1158,
1017, 768 cm.sup.-1; .sup.1H NMR .delta. 0.71 (3H), 1.22 (3H), 1.23
(3H), 1.41-1.53 (1H), 1.67-1.86 (1H), 2.26-2.60 (4H), 2.71-2.87
(2H), 2.96-3.08 (1H), 4.46-4.62 (1H); 5.26 (2H), 5.43 (1H), 6.98
(2H), 7.13 (2H), 7.23-7.28 (1H), 7.37 (2H); .sup.13C NMR .delta.
20.22, 21.66, 22.29, 22.91, 31.91, 37.19, 39.64, 46.44, 48.57,
54.26, 56.48, 65.14, 115.09, 128.37, 129.62, 130.23, 130.33,
131.98, 136.90, 157.72, 171.75, 178.54, 182.19; MS (FAB) m/z 536,
495, 464, 449, 431, 418, 386, 353, 336, 287, 236; H.sub.2O (Karl
Fischer) 0.70%; Anal. C, 60.08; H, 5.86; Cl, 13.05; N, 2.70 (calcd
corrected for H.sub.2O: C, 60.03; H, 5.86; Cl 13.13; N, 2.59).
Example 202
(1S-cis)-1-[(3,4-Dichlorophenyl)methyl]-N-[[3-[(1,1-dimethylethoxy)carbony-
l]-2,2,3-trimethylcyclopentyl]carbonyl]-L-histidine
(C.sub.27H.sub.35Cl.sub.2N.sub.3O.sub.5)
[0667] 294
[0668] To
(1S-cis)-N-([3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcyc-
lopentyl]carbonyl]-1-[(3,4-dichlorophenyl)methyl]-L-histidine
methyl ester (0.5 g, 0.88 mmol) in H.sub.2O (10 mL) was added
LiOH.H.sub.2O (10 equiv, 8.8 mmol, 0.37 g) in cold H.sub.2O (10
mL). Stirred reaction overnight. Lowered pH of reaction solution to
5 with 1N HCl. Filtered resulting precipitate, washed precipitate
with H.sub.2O and dried precipitate under hi vacuum conditions to
yield 0.46 g (95%) of (1S-cis)-1-[(3,4-Dichloroph-
enyl)methyl]-N-[[[3-[(1,1-dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopen-
tyl]carbonyl]-L-histidine Example 202 (I-c; where
R.sup.2a=(1,1-dimethylet- hoxy)carbonyl, R.sup.1=CH.sub.3,
R.sup.3=H, R.sup.4=H, R.sup.5=CO.sub.2H, and
R.sup.6=1-(3,4-dichlorophenyl)methyl]-4-imidazolyl, n=1,
Stereochemistry=(1S-cis)-L): mp 234-235.degree. C.
Example 203
(1S-cis)-N-[[(3-Methoxymethoxymethyl)-2,2,3-trimethylcyclopentyl]carbonyl]-
-O-[(2,6-dichlorophenyl)methyl]-L-r-yrosine
(C.sub.27H.sub.33Cl.sub.2NO.su- b.5)
[0669] 295
[0670] The synthesis of Example 203 is taught by Scheme 10 under
the heading Preparation of Example 203.
Example 204
[1R-[1.alpha.(S*)3.alpha.]]-.beta.-[[(3-Carboxy-1,2,2-trimethylcyclopentyl-
)carbonyl]amino]-4-[(2,6-dichlorophenyl)methoxy]-benzenebutanoic
Acid (C.sub.27H.sub.31Cl.sub.2NO.sub.6)
[0671] 296
[0672] The synthesis of Example 204 is taught by scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=R.sup.4=H, R.sup.3=Me,
R.sup.5-CH.sub.2CO.sub.2H,
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl-- , n=1,
Stereochemistry=[1R-[1.alpha.(S*),3.alpha.]]. Accordingly,
[1R-[1.alpha.(S*),3.alpha.]]-.beta.-[[(3-Carboxy-1,2,2-trimethylcyclopent-
yl)carbonyl]amino]-4-[(2,6-dichlorophenyl)methoxy]benzenebutanoic
acid (C.sub.27H.sub.31Cl.sub.2NO.sub.6) is obtained from 30-B and
(1S)-camphoric anhydride (33-A) by the procedures taught by Scheme
1: mp 106-108.degree. C.; TLC R.sub.f=0.24 (250:250:1
hexanes/EtOAc/HCO.sub.2H) [a].sub.D=+13 (MeOH); IR 3144, 3057,
3030, 2954, 2924, 2870, 2855, 1706, 1027, 1612, 1585, 1565, 1511,
1466, 1439, 1377, 1299, 1241, 1197, 1178, 1017, 1000, 778, 768
cm.sup.-1; .sup.1H NMR .delta. 0.70 (3H), 1.16 (3H), 1.16 (3H),
1.30 (3H), 1.43-1.61 (1H), 1.77-1.97 (1H), 2.09-2.32 (2H),
2.36-2.49 (1H), 2.57-2.68 (1H), 2.69-2.83 (2H), 2.90-3.02 (1H),
4.44-4.59 (1H), 5.25 (2H), 5.73 (1H), 6.97 (2H), 7.11 (2H),
7.22-7.26 (3H), 7.37 (2H); .sup.13C NMR .delta. 20.88, 21.16,
22.04, 23.15, 32.57, 37.76, 39.59, 47.39, 48.16, 52.44, 55.73,
65.17, 86.13, 115.02, 128.37, 129.56, 130.34, 132.00, 136.89,
157.72, 174.53, 178.42, 181.12; MS (FAB) m/z 536, 518, 490, 378,
354, 336, 294, 159, 137, 109, 88, 69, 55; H.sub.2O (Karl Fischer)
0.52%; Anal. C, 60.07; H, 5.84; Cl, 13.03; N, 2.71 (calcd corrected
for H.sub.2O: C, 60.14; H, 5.85; Cl, 13.15; N, 2.60),
Example 205
(1S-cis)-O-[(2,6-Dichlorophenyl)methyl]-N-[[3-(methoxycarbonyl)-2,2,3-trim-
ethylcyclopentyl]carbonyl]-L-tyrosine
(C.sub.27H.sub.31Cl.sub.2NO.sub.5)
[0673] 297
[0674] Example 205 is taught by Scheme 20, 20-D: wherein R.sup.4=H,
R.sup.5=CO.sub.2H, R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]-phenyl
and Stereochemistry=[1S-cis]-L.
[0675] To a solution of the diester 20-D (R.sup.4=H,
R.sup.5=CO.sub.2CH.sub.3;
R.sup.6=4-[(2,6-Dichlorophenyl)methoxy]phenyl,
Stereochemistry=(1S-cis)-L (0.32 g, 0.58 mmol) in methanol (5 mL)
is added a solution of LiOH.H.sub.2O (0.31 g, 7.4 mmol) in H.sub.2O
(5 mL). After overnight stirring, the mixture is diluted with water
(50 mL) and evaporated in vacuo until the methanol is gone. The
aqueous solution is cooled in an ice bath and brought to pH 4 using
1N HCl, resulting in a white precipitate which is chromatographed
on silica gel (10% methanol/chloroform) to give a white solid (0.25
g, 80% yield).
[0676] .sup.1H NMR (D.sub.6DMSO) 7.85 (m, 1H), 7.58-7.44 (m, 3H),
7.19 (m, 2H), 6.96 (m, 2H), 5.18 (s, 2H), 4.43 (m, 1H), 3.59 (s,
3H), 3.03-2.81 (m, 2H), 2.69 (m, 1H), 2.40 (m, 1H), 1.91 (m, 1H),
1.56 (m, 1H), 1.39 (m, 1H), 1.16 (s, 3H), 1.31 (s, 3H), 0.59 (s,
3H); IP (mull) 1727, 1639, 1612, 1585, 1565, 1511, 1439, 1298,
1241, 1197, 1179, 1160, 1119, 779, 768; MS (FAB) m/z (rel.
intensity) 536 (M+H, 99), 538 (66), 537 (39), 536 (99), 322 (22),
197 (27), 169 (21), 159 (29), 137 (31), 109 (66), 107 (22).
Example 206
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[2,2,3-trimethyl-3-[[(phenylmeth-
oxy)amino]-carbonyl]cyclopentyl]carbonyl]-L-phenylalanine Methyl
Ester (C.sub.34H.sub.37Cl.sub.2N.sub.3O.sub.6)
[0677] 298
[0678] The synthesis of Example 206 is taught by Scheme 23 under
the heading Preparation 23-C.
Example 207
(1S-cis)-N-[[3-[[(2-Cyanoethyl)amino]carbonyl]-2,2,3-trimethylcyclopentyl]-
carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine Methyl
Ester
[0679] 299
[0680] The synthesis of Example 207 is taught by Scheme 22 under
the heading Preparation of 22-C.
Example 208
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[(2-chloro--
3-pyridinyl)carbonyl]amino]-L-phenylalanine
(C.sub.25H.sub.28ClN.sub.3O.su- b.6)
[0681] 300
[0682] The synthesis of Example 208 is taught by Scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=Me, R.sup.3=R.sup.4=H,
R.sup.5=--CO.sub.2H,
R.sup.6=4-[[(2-chloro-3-pyridyl)carbonyl]amino]phenyl, n=1,
Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis)-N-[(3-Carboxy-2,2,3-tri-
methylcyclopentyl)carbonyl3-4-[[(2-chloro-3-pyridinyl)carbonyl]amino]-L-ph-
enylalanine (C.sub.25H.sub.28ClN.sub.3O.sub.6) is prepared from
31-C, 15-D and 2-chloro-3-nicotinic acid by the procedures taught
by Scheme 2: mp 172-174.degree. C.; TLC silica gel R.sub.f=0.17
(750:250:3 EtOAc/hexanes/HCO.sub.2H); IR (mineral oil mull) 3410,
3289, 3193, 3124, 3050, 2955, 2924, 2868, 2855, 1714, 1655, 1606,
1582, 1537, 1516, 1461, 1415, 1401, 1377, 1329, 1279, 1257, 1242,
1207, 1187, 1152 cm.sup.-1; .sup.1H NMR .delta. 0.80 (3H),
1.13-1.28 (1H), 1.16 (3H), 1.19 (3H), 1.38-1.50 (1H), 1.67-1.83
(1H), 2.08-2.23 (1H), 2.45-2.58 (2H), 3.04-3.24 (2H), 4.75-4.87
(1H), 5.96 (1H), 7.12 (2H), 7.32 (1H), 7.56 (2H), 7.96 (1H), 8.43
(1H), 9.32 (1H); MS (FAB) m/z 504, 502, 484, 468, 456, 371, 320,
302, 274, 140, 109; Anal. C, 57.54; H, 5.84; Cl, 6.79; N, 8.05
(calcd corrected for H.sub.2O. C, 57.55; H, 5.83; Cl, 6.79; N,
8.08).
Example 209
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[(2,6-dichl-
oro-3-pyridinyl)carbonyl]amino]-L-phenylalanine
(C.sub.25H.sub.27Cl.sub.2N- .sub.3O.sub.6)
[0683] 301
[0684] The synthesis of Example 209 is taught by Scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=Me, R.sup.3=R.sup.4=H,
R.sup.5=--CO.sub.2H,
R.sup.6=4-[[(2,6-dichloro-3-pyridyl)carbonyl]amino]phenyl, n=1,
Stereochemistry=(1S-cis)-L. Accordingly,
(1S-cis)-N-((3-Carboxy-2,2,3-tri-
methylcyclopentyl)carbonyl]-4-[[(2,6-dichloro-3-pyridinyl)carbonyl]amino]--
L-phenylalanine (C.sub.25H.sub.27Cl.sub.2N.sub.3O.sub.6) is
prepared from 31-C, 15-D and 2,6-dichloro-3-nicotinic acid as
taught by Scheme 2 and by the synthesis of Example 208: mp
246-247.degree. C.; TLC silica gel R.sub.f=0.28 (750:250:2
EtOAc/hexanes/HCO.sub.2H); IR (mineral oil mull) 3292, 3196, 3125,
3059, 2954, 2923, 2855, 1712, 1656, 1607, 1575, 1544, 1515, 1461,
1426, 1414, 1377, 1343, 1329, 1272, 1244, 1206, 1186, 1160, 1144
cm.sup.-1; .sup.1H NMR .delta. 0.65 (3H), 1.10 (3H), 1.15 (3H),
1.24-1.37 (1H), 1.43-1.62 (1H), 1.83-1.97 (1H), 2.26-2.45 (1H),
2.67 (1H), 2.82-3.04 (2H), 3.30 (3H), 4.38-4.49 (1H), 7.21 (2 H),
7.55 (2H), 7.71 (1H), 7.80 (1H), 8.14 (1H), 10.63 (1H); MS (FAB)
m/z 538, 536, 538, 518, 490, 371, 354, 336, 281, 200, 174, 137,
109.
Example 210
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[(2-methoxy-
-6-chloropyridin-3-yl)carbonyl]-amino]-L-phenylalanine
[0685] 302
[0686] The synthesis of Example 210 is taught by Scheme 2, I-c:
wherein R.sup.2a=--CO.sub.2H, R.sup.1=Me, R.sup.3=R.sup.4=H,
R.sup.5=--CO.sub.2H,
R.sup.6=4-[[(6-chloro-2-methoxy-3-pyridyl)carbonyl]amino]phenyl,
n=1, Stereochemistry=(1S-cis)-L. Accordingly, Example 210 is
synthesized as follows:
[0687] A solution of the dimethyl ester of Example 209 (0.098 g,
0.18 mmol) in 4:1 THF/MeOH is reacted at room temperature under Ar
with an aqueous solution of LiOH:H.sub.2O (0.042 g, 1.0 mmol). The
reaction mixture is stirred for 2.5 h. It is diluted with H.sub.2O,
acidified with aq HCl, and extracted with EtOAc. The combined
extracts are washed with brine, dried, filtered and concentrated to
an oil that is purified by silica gel flash chromatography
(650:350:4 EtOAc/hexanes/HCO.sub.2H). The product is azeotroped
thrice from toluene, diluted with CH.sub.3CN/H.sub.2O, and
lyophilized to give 0.043 g (0.081 mmol, 45%) of Example 210 as a
white solid: mp 128-131 C; TLC silica gel R.sub.f=0.13 (500:500:2
EtOAc/hexanes/HCO.sub.2H); .sup.1H NMR .delta. 0.64 (3H), 1.10
(3H), 1.15 (3H), 1.23-1.38 (1H), 1.44-1.53 (m, 1H), 1.78-1.97 (m,
1H), 2.66 (t, 2H, J=9.4), 2.78-3.07 (m, 2H), 3.67-3.84 (1H), 3.96
(3H), 4.37-4.46 (1H), 7.19 (2H), 7.57 (2H), 7,78 (2H), 8.05 (1H),
10.18 (1H); MS (FAB) m/z 534, 532, 514, 486, 371, 360, 350, 332,
304, 275, 190, 127, 109.
Example 211
[1S-[1.alpha.,3.alpha.(R*)]]-N-[[[3-[[(1-Carboxyethyl)amino]carbonyl]-2,2,-
3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylal-
anine
[0688] 303
[0689] The synthesis of Example 211 is taught by Scheme 19 under
the heading Preparation of Example 211.
Example 212
[1S-[1.alpha.,3.alpha.((S*)
1-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidinyl]carb-
onyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(2,6-dichlorobenzoyl)amino]-
-L-phenylalanine Monolithium Salt
[0690] 304
[0691] The synthesis of Example 212 is taught by Scheme 19 under
the heading Preparation of Example 212.
Example 213
[1S-[1.alpha.,3.alpha.(R*)]]-N-[3-[[2-(Aminocarbonyl)-1-pyrrolidinyl]carbo-
nyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-
-phenylalanine Monosodium Salt
[0692] 305
[0693] The synthesis of Example 213 is taught by Scheme 19 under
the heading Preparation of Example 213.
Example 214
(1S-cis)-N-[[3-[[(2-Carboxyethyl)amino]carbonyl]-2,2,3-trimethylcyclopenty-
l]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.29H.sub.33Cl.sub.2N.sub.3O.sub.7)
[0694] 306
[0695] The synthesis for Example 214 is taught by Scheme 19 under
the heading Preparation of Example 214.
Example 215
[1S-[1.alpha.,3.alpha.(R*)]]-N-[3-[[2-[(Methylamino)carbonyl]-1-pyrrolidin-
yl]carbonyl]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-dichlorobenzoyl)-
amino]-L-phenylalanine Monosodium Salt
[0696] 307
[0697] The synthesis for Example 215 is taught by Scheme 19 under
the heading Preparation of Example 215.
Example 216
(1S-cis)-N-[[3-[[(2-Cyanoethyl)amino]carbonyl]-2,2,3-trimethylcyclopentyl]-
carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
[0698] 308
[0699] The synthesis of Example 216 is taught by Scheme 22 under
the heading Preparation of Example 216
Example 217
(1S-cis)-4-((2,6-Dichlorobenzoyl)amino]-N-[[2,2,3-trimethyl-3-[[(phenylmet-
hoxy)amino]-carbonyl]cyclopentyl]carbonyl]-L-phenylalanine
(C.sub.33H.sub.35Cl.sub.2N.sub.3O.sub.6)
[0700] 309
[0701] The synthesis for Example 217 is taught by Scheme 23 under
the heading Preparation of Example 217.
Example 218
(1S-cis)-4-[(2,6-}
Dichlorobenzoyl)amino]-N-[[3-[(hydroxyamino)carbonyl]-2-
,2,3-trimethylcyclopentyl]carbonyl]-L-phenylalanine Methyl
Ester
[0702] 310
[0703] The synthesis for Example 218 is taught by Scheme 23 under
the heading Preparation of Example 218.
Example 219
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[3-[(hydroxyamino)carbonyl]-2,2-
,3-trimethylcyclopentyl]carbonyl]-L-phenylalanine
[0704] 311
[0705] The synthesis for Example 219 is taught by Scheme 22 under
the heading Preparation of Example 219.
Example 220
(1S-cis)-N-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[[(1,1'-bi-
phenyl)-4-yl]amino]-carbonyl]-L-phenylalanine
[0706] 312
[0707] The synthesis for Example 220 is taught by Schemes 35 and 2
under the heading Preparation of Example 220.
Example 221
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(4-chloroph-
enyl)amino]carbonyl]-L-phenylalanine
[0708] 313
[0709] The synthesis for Example 221 is taught by Schemes 35 and 2
under the heading Preparation of Example 221
Example 222
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(2-trifluor-
omethylphenyl)amino]carbonyl]-L-phenylalanine
[0710] 314
[0711] The synthesis for Example 222 is taught by Schemes 35 and 2
under the heading Preparation of Example 222.
Example 223
(1S-cis)-[[(3-Carboxy-2,2,3-trimethylcyclopentyl]carbonyl]-4-[[(2,4,6-tric-
hlorophenyl)amino]-carbonyl]-L-phenylalanine
[0712] 315
[0713] The synthesis for Example 223 is taught by Schemes 35 and 2
under the heading Preparation of Example 223.
Example 224
[1S-[160
(R*),3.alpha.]]-4-[[[(1-Carboxy-3-methylbutyl]amino]carbonyl]-N-[-
[(3-carboxy-2,2,3-trimethylcyclopentyl]-carbonyl]-L-phenylalanine
[0714] 316
[0715] The synthesis for Example 224 is taught by Schemes 35 and 2
under the heading Preparation of Example 224.
Example 225
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[[2,2,3-trimethyl-3-[(4-morpholi-
nyl)carbonyl]-cyclopentyl]carbonyl]-L-phenylalanine
[0716] 317
[0717] The preparation of Example 225 is taught by Scheme 27.
Example 226
(1S-cis)-4-[(2,6-Dichlorobenzoyl)amino]-N-[2,2,3-trimethyl-3-[(4-morpholin-
yl)carbonyl]-cyclopentyl]carbonyl]-L-phenylalanine Methyl Ester
[0718] 318
[0719] The preparation of Example 226 is taught by Scheme 27.
Example 227
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-4-[[[(2,6-dichlorophenyl)amino]carbonyl]amino]-L-phenylalanine
Methyl Ester
[0720] 319
[0721] The synthesis of Example 227 is taught by Scheme 26.
Example 228
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[[(2,6-dich-
lorophenyl)amino]carbonyl]amino]-L-phenylalanine Methyl Ester
[0722] 320
[0723] The synthesis of Example 228 is taught by Scheme 26.
Example 229
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[[(2,6-dich-
lorophenyl)amino]carbonyl]amino]-L-phenylalanine
[0724] 321
[0725] The synthesis of Example 229 is taught by Scheme 26
Example 230
(1S-cis)-N-[[3-[(1,1-Dimethylethoxy)carbonyl]-2,2,3-trimethylcyclopentyl]c-
arbonyl]-4-[(2-chloro-5-trifluoromethylbenzoyl)amino]-L-phenylalanine
Methyl Ester
[0726] 322
[0727] Example is 230 synthesized in the same manner as Example
54.
Example 231
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2-chloro-5-
-trifluoromethylbenzoyl)amino]-L-phenylalanine Methyl Ester
[0728] 323
[0729] Example 231 is synthesized in the same manner as Example
54.
Example 232
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2-chloro-5-
-trifluoromethylbenzoyl)amino]-L-phenylalanine
[0730] 324
[0731] Example 232 is synthesized in the same manner as Example
54.
Example 233
(1S-cis)-N-[[3-[[(2-Amino-2-oxoethyl)amino]carbonyl]-2,2,3-trimethylcyclop-
entyl]carbonyl]-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine
Methyl Ester
[0732] 325
[0733] The synthesis for Example 233 is taught by Scheme 8 under
the heading Preparation of 8-F.
Example 234
[S-[1.alpha.(R)
3.alpha.]]-2-[[[3-Carboxy-2,2,3-trimethylcyclopentyl]carbo-
nyl]amino]-6-[(2,6-dichlorobenzoyl)amino] Hexanoic Acid
[0734] 326
[0735] Example 234 was prepared according to Scheme 2. Physical
properties as follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta.
7.36 (3H), 4.29 (1H), 3.24 (2H) 2.71 (1H), 2.45 (1H), 2.04 (1H),
1.71 (8H), 1.23 (3H), 1.13 (3H), 0.75 (3H); .sup.13C NMR (75 MHz,
CD.sub.3OD) .delta. 176.74, 173.06, 164.47, 137.20, 131.64, 130.84,
128.05, 55.91, 52.87, 46.16, 38.50, 32.31, 30.44, 28.43, 22.64,
22.32, 21.92, 21.07, 20.57; MS (FAB) m/z (rel. intensity) 501
(M.sup.+, 22), 504 (20), 503 (32), 502 (26), 501 (22), 109 (17), 73
(99), 69 (25), 57 (27), 55 (23); MS (ES-) for
C.sub.23H.sub.30Cl.sub.2N.sub.2O.sub.6 m/z 499.3 (M-H).sup.-; Anal.
Calcd for C.sub.23H.sub.30Cl.sub.2N.sub.2O.sub.6.0.25H.sub.2O: C,
54.60; H. 6.07; N, 5.53. Found: C, 54.58; H, 6.14; N, 5.45.
Example 235
[1S-[1.alpha.(R*),3.alpha.]]-2-[[[3-Carboxy-2,2,3-trimethylcyclopentyl]car-
bonyl]amino]-5-[(2,6-dichlorobenzoyl)amino] Pentanoic Acid
[0736] 327
[0737] Example 235 was prepared according to Scheme 2. Physical
properties as follows: .sup.1H NMR (300 MHz, CD.sub.3OD) .delta.
7.38 (3H), 4.41 (1H), 3.40 (2H) 2.83 (1H), 2.53 (1H), 2.08 (1H),
1.79 (3H), 1.47 (1H), 1.30 (3H), 1.24 (3H), 0.83 (3H); .sup.13C NMR
(75 MHz, CD.sub.3OD) .delta. 178.23, 174.24, 173.83, 165.84,
136.07, 131.79, 130.67, 127.80, 56.06, 52.92, 52.10, 38.84, 32.35,
28.57, 25.43, 22.33, 21.61, 20.95, 20.50; MS (FAB) m/z (rel.
intensity) 487 (M.sup.+, 99), 490 (14), 489 (67), 488 (25), 487
(99), 305 (19), 242 (16), 175 (12), 173 (18); Anal. Calcd for
C.sub.22H.sub.28Cl.sub.2N.sub.2O.sub.6: C, 54.22; H, 5.79; N, 5.75.
Found: C, 53.91; H, 5.93; N, 5.43.
Example 236
[1S-[1.alpha.,3.alpha.(1S*,3R*)]]-N-[[3-[[[(3-Carboxy-1,2,2-trimethylcyclo-
pentyl)amino]carbonyl]-amino]-2,2,3-trimethylcyclopentyl]carbonyl]-4-[(2,6-
-dichlorobenzoyl)-amino]-L-phenylalanine
[0738] 328
[0739] The synthesis of Example 236 is taught by Schemes 28 and
29.
Example 237
(1S-cis)-N-[(3-Carboxy-2,2,3-triwethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0740] 329
[0741] The synthesis for Example 237 is taught by Scheme 37 under
the heading Preparation of Example 237.
Example 238
(1S-trans)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0742] 330
[0743] The synthesis for Example 238 is taught by Scheme 37 under
the heading Preparation of Example 238.
Example 239
(1S-trans)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0744] 331
[0745] The synthesis for Example 239 is taught by Scheme 37 under
the heading Preparation of Example 239.
Example 240
(1R-trans)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0746] 332
[0747] The synthesis of Example 240 is taught by Scheme 37 under
the heading Preparation of Example 240.
Example 241
(1R-trans)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0748] 333
[0749] The synthesis of Example 241 is taught by Scheme 37 under
the heading Preparation of Example 241.
Example 242
(1R-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0750] 334
[0751] The synthesis of Example 242 is taught by Scheme 37 under
the heading Preparation of Example 242.
Example 243
(1R-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0752] 335
[0753] The synthesis of Example 243 is taught by Scheme 37 under
the heading Preparation of Example 243.
Example 244
(1S-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0754] 336
[0755] The synthesis of Example 244 is taught by Scheme 37 under
the heading Preparation of Example 244.
Example 245
(1S--cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichl-
orobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0756] 337
[0757] The synthesis of Example 245 is taught by Scheme 37 under
the heading Preparation of Example 245.
Example 246
(1S-trans)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0758] 338
[0759] The synthesis of Example 246 is taught by Scheme 37 under
the heading Preparation of Example 246.
Example 247
(1S-trans)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl.)carbonyl]-4-[(2,6-dic-
hlorobenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.- 6)
[0760] 339
[0761] The synthesis of Example 247 is taught by Scheme 37 under
the heading Preparation of Example 247
Example 248
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0762] 340
[0763] The synthesis of Example 248 is taught by Scheme 37 under
the heading Preparation of Example 248.
Example 249
(1R-cis)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dichlo-
robenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0764] 341
[0765] The synthesis of Example 249 is taught by Scheme 37 under
the heading Preparation of Example 249.
Example 250
(1R-trans)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0766] 342
[0767] The synthesis of Example 250 is taught by Scheme 37 under
the heading Preparation of Example 250.
Example 251
(1R-trans)-N-[(3-Carboxy-1,2,2-trimethylcyclopentyl)carbonyl]-4-[(2,6-dich-
lorobenzoyl)amino]-D-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O.sub.6- )
[0768] 343
[0769] The synthesis of Example 251 is taught by Scheme 37 under
the heading Preparation of Example 251.
Example 252
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-3-bromo-4-[(2,-
6-dichlorobenzoyl)amino]-L-phenylalanine
(C.sub.26H.sub.28Cl.sub.2N.sub.2O- .sub.6)
[0770] 344
[0771] The synthesis for Example 252 is taught by Scheme 2 (Method
B) and is explained under the heading Preparation of Example
252.
Example 253
(1S-cis)-N-[(3-Carboxy-2,2,3-trimethylcyclopentyl)carbonyl]-4-[[2-chloro-4-
-[(1,1-dimethyl)ethyl]benzoyl]amino]-L-phenylalanine
(C.sub.30H.sub.37Cl.sub.1N.sub.2O.sub.6)
[0772] 345
[0773] The synthesis of Example 253 is taught by Scheme 38 under
the heading Preparation of Example 253.
Example 254
[1S-[1.alpha.(R*),3.alpha.]]-.alpha.-[[(3-Carboxy-2,2,3-trimethylcyclopent-
yl)carbonyl]amino-]-3-[(2,6-dichlorophenyl)methoxy]-6-pyridinepropanoic
Acid (C.sub.25H.sub.28Cl.sub.2N.sub.2O.sub.6)
[0774] 346
[0775] The synthesis of Example 254 is taught by Scheme 39 under
the heading Preparation of Example 254.
REFERENCE EXAMPLES (R. EXAMPLES)
R. Example 1
(1R)-1,8,8-Trimethyl-3-oxabicyclo[3.2.1]octane-2,4-dione
[(1R)-camphoric Anhydride]
[0776] 347
[0777] Method A:
[0778] A mixture of
(1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid)
[(1R,3S)-camphoric acid] (40.0 g), AcCl (23.5 g), and Ac.sub.2O
(81.6 g) was heated under reflux for 3 hr. The mixture was
concentrated in vacuo, dissolved in CHCl.sub.31 washed with sat.
NaHCO.sub.3, brine, and dried over Na.sub.2SO.sub.4. The solvent
was removed in vacuo and the residue was triturated with
i-Pr.sub.2O to give (1R)-1,8,8-Trimethyl-3-ox-
abicyclo[3.2.1]octane-2,4-dione [(1R)-camphoric anhydride (34.0 g)
as crystals.
[0779] Method B:
[0780] DIEA (8.0 g) was added to a suspension of (1R,3S)-camphoric
acid (5.0 g) and BOP Reagent (11.1 g) in THF (50 ml) at room
temperature. The mixture was stirred for 4 hr. and the solvent was
removed in vacuo. The residue was extracted with AcOEt and the
extract was washed with 5Q HCl, sat. NaHCO.sub.3, and sat. LiCl,
and dried over Na.sub.2SO.sub.4. The solvent was removed in vacuo
and the residue was purified by flash chromatography on silica gel
(eluent; CHCl.sub.3) to give (1R)-camphoric anhydride (4.1 g) as a
colorless powder.
R. Example 2
(1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid
3-(1,1-dimethylethyl) Ester
[0781] 348
[0782] To a solution of (1R)-camphoric anhydride (0.18 g) in THF (2
ml) was added 1M t-BuOK in THF (1 ml) at -78.degree. C. under
N.sub.2 and the mixture was stirred for 10 hr. at the same
temperature. The mixture was concentrated in vacuo, dissolved in
H.sub.2O, and extracted with AcOEt. The aqueous layer was acidified
with 1N HCl to pH 3 and extracted with CH.sub.2Cl.sub.2. The
extract was washed with brine and dried over Na.sub.2SO.sub.4. The
solvent was removed in vacuo to give
(1R-cis)-1,2,2-Trimethylcyclopentan-1,3-dicarboxylic acid
3-dimethylethyl ester (0.24 g).
R. Example 3
(1R-cis)-3-Formyl-1,2,2-trimethylcyclopentanecarboxylic Acid
[0783] 349
[0784] A mixture of (1R)-camphoric anhydride (1.76 g),
N,O-dimethylhydroxylamine hydrochloride (1.13 g), DIEA (8.4 ml) in
THF (15 ml) was heated for 3 hr. at 85.degree. C. in a sealed tube.
After cooling, the tube was opened and 1N HCl (75 ml) was added.
The resulting mixture was extracted with AcOEt.
[0785] The extract was dried over Na.sub.2SO.sub.4 and the solvent
was removed in vacuo. The residue was purified by column
chromatography on silica gel to give
(1R,3S)-3-(N-methoxy-N-methylcarbamoyl)-1,2,2-trimethy-
lcyclopentanecarboxylic acid (800 mg).
[0786] To a solution of the obtained compound (215 mg) in THF (5
ml) was added a 1M solution of LiAlH.sub.4 in THF (1.5 ml) at
-78.degree. C. The mixture was stirred for 1 hr. at -78.degree. C.,
warmed to 0.degree. C. and quenched with 1N HCl. The resulting
mixture was extracted with AcOEt. The extract was dried over
Na.sub.2SO.sub.4 and the solvent was removed in vacuo to give a 3:1
mixture of (1R-cis)-3-Formyl-1,2,2-trimethylcyclop-
entanecarboxylic acid (aldehyde form) and
(1R,5S)-4-hydroxy-1,8,8-trimethy- l-3-oxabicyclo
[32.1]-octane-2-one (acetal form) (153 mg). Both forms are
exchangeable with each other in solution.
R. Example 4
O-(Cyclohexylmethyl)-L-tyrosine Methyl Ester, Hydrochloride
Salt
[0787] 350
[0788] To a mixture of N-(tert-butoxycarbonyl)-L-tyrosine methyl
ester (356 mg), K.sub.2CO.sub.3 (830 mg), n-Bu.sub.4NI (89 mg) in
DMF (5 ml) was added cyclohexylmethyl bromide (202 , l) and the
mixture was stirred for 1 day at room temperature. After addition
of brine (40 ml), the resulting mixture was extracted with AcOEt.
The extract was dried over Na.sub.2SO.sub.4 and the solvent was
removed in vacuo. The residue was purified by column chromatography
on silica gel (eluent; 9:1, Hexane/AcOEt.fwdarw.1:1, Hexane/AcOEt)
to give N-(tert-butoxycarbonyl)-O-- cyclohexylmethyl-L-tyrosine
methyl ester (470 mg). The obtained compound (347 mg) was dissolved
in 3N HCl/AcOt (5 ml) and the mixture was stirred overnight. The
solvent and excess HCl was removed in vacuo to give
O-(Cyclohexylmethyl)-L-tyrosine methyl ester, hydrochloride
salt.
R. Example 5
1-[(2,4-Dichlorophenyl)methyl]-L-histidine Methyl Ester,
Dihydrochloride Salt
[0789] 351
[0790] N-(tert-butoxycarbonyl)-L-histidine methyl ester (1.0 g) was
added to a solution of 2,4-dichiorobenzyl chloride (0.73 g) and
Et.sub.3N (0.27 g) in benzene (10 ml) and the mixture was heated
under reflux for 1 hr. The mixture was cooled and the precipitate
was removed by filtration. The filtrate was washed with H.sub.2O,
dried over Na.sub.2SO.sub.4, and the solvent was removed in vacuo.
The residue was purified by column chromatography on silica gel
(eluent; 95:5, CHCl.sub.3/MeOH) to give
N-(tert-butoxycarbonyl)-1-[(2,4-dichlorophenyl)methyl]-L-histidine
methyl ester. The obtained compound was treated in a similar manner
as described in R. Example 4 to give
1-[(2,4-Dichlorophenyl)methyl]-L-histidine methyl ester,
dihydrochloride salt.
R. Example 6
1-[(2,6-Dichlorophenyl)methyl]-L-histidine methyl ester,
dihydrochloride salt.
[0791] 352
[0792] L-histidine (3.13 g) was added to a solution of NaNH.sub.2
in liquid ammonia prepared from Na (0.93 g) and FeCl.sub.3
(catalytic amount) in liquid ammonia (50 ml) After 15 min., a
solution of 2,6-dichlorobenzylchloride (3.96 g) in THF (5 ml) was
added and the mixture was stirred for 3 hr. The reaction mixture
was quenched with H.sub.2O and ether. The pH of the aqueous layer
was adjusted to pH 8 with 5% HCl followed by cooling. The resulting
precipitate was collected by filtration, washed with H.sub.2O, and
dried to give 1-[(2,6-dichlorophenyl)methyl]-L-histidine (3.58 g).
The obtained compound (0.80 g) was dissolved in MeOH (30 ml) and
HCl gas was bubbled for 10 min. at 0.degree. C. The reaction
mixture was stirred for 15 hr. at room temperature and the solvent
was removed in vacuo to give
1-[(2,6-Dichlorophenyl)methyl]-L-histidine methyl ester,
dihydrochloride salt.
R. Example 7
3-Nitro-L-tyrosine Methyl Ester, Hydrochloride Salt
[0793] 353
[0794] To a solution of N-(tert-butoxycarbonyl)-L-tyrosine methyl
ester (5.64 g) in THF (10 ml) was added NH.sub.4NO.sub.3 (3 g) and
concentrated HNO.sub.3 (3 ml). After 30 sec., the reaction mixture
turned to dark red with reflux occurring. The reaction mixture was
quenched with solid NaHCO.sub.3 and H.sub.2O, and extracted with
AcOEt. The extract was dried over Na.sub.2SO.sub.4 and the solvent
was removed in vacuo. The residue was purified by column
chromatography on silica gel (eluent; Hexane.fwdarw.1:1,
Hexane/AcOEt) to give N-(tert-butoxycarbonyl)-3-nitro-- L-tyrosine
methyl ester (3.42 g). The obtained compound was treated in a
similar manner as described in R. Example 4 to give
3-Nitro-L-tyrosine methyl ester, hydrochloride salt.
R. Example 8
3-[(2,6-Dichlorobenzoyl)amino]-L-tyrosine Methyl Ester,
Hydrochloride Salt
[0795] 354
[0796] A mixture of N-(tert-butoxycarbonyl)-3-nitro-L-tyrosine
methyl ester (2.0 g) and 10% Pd-C (1.0 g) in MeOH (10 ml) was
subjected to catalytic hydrogenolysis at atmospheric pressure. The
catalyst was filtered off and the filtrate was evaporated in vacuo.
The residue was triturated with ether to give
3-amino-N-(tert-butoxycarbonyl)-L-tyrosine methyl ester (1.5 g) as
a solid. To a solution of the obtained compound (1.0 g) in
CH.sub.2Cl.sub.2 (10 ml) was added 2,6-dichlorobenzoyl chloride
(0.74 g) and DIEA (1.1 g) and the mixture was stirred at room
temperature overnight. The solvent was removed in vacuo and the
residue was purified by column chromatography on silica gel
(eluent; 2:1, Hexane/AcOEt) to give
N-(tert-butoxycarbonyl)-3-(2,6-dichlorobenzamido)-L- -tyrosine
methyl ester (1.2 g). The obtained compound was treated in a
similar manner as described in R. Example 4 to give
3-[(2,6-Dichlorobenzoyl)amino]-L-tyrosine methyl ester,
hydrochloride salt.
R. Example 9
4-[[(2,6-Dichlorophenyl)methyl]amino]-L-phenylalanine Methyl Ester,
Hydrochloride Salt
[0797] 355
[0798] To a mixture of
4-amino-N-(tert-butoxycarbonyl)-L-phenylalanine methyl ester (0.59
g) and 2,6-dichlorobenzaldehyde (0.35 g) in MeOH (9 ml) was added
NaCNBH.sub.3 (0.38 g), AcOH (1 ml), and molecular sieves 4A
(catalytic amount) and the mixture was stirred overnight at room
temperature.
[0799] The reaction mixture was quenched with brine and 1N HCl. The
solvent was removed in vacuo and the residue was extracted with
AcOEt. The extract was washed with 1N HCl, brine, dried over
MgSO.sub.4, and the solvent was removed in vacuo. The residue was
purified by column chromatography on silica gel (eluent; 10%
EtOH/CH.sub.2Cl.sub.2) to give
N-(tert-butoxycarbonyl)-4-[(2,6-dichlorophenyl)methyl]amino]-L-phenylalan-
ine (0.36 g) as a colorless solid. The obtained compound (0.5 g)
was dissolved in MeOH (5 ml) and HCl gas was bubbled for 5 min. at
0.degree. C. The mixture was stirred for 2 hr. and the solvent was
removed in vacuo to give
4-[[(2,6-Dichlorophenyl)methyl]amino]-L-phenylalanine methyl ester,
hydrochloride salt.
R. Example 10
(S)-.alpha.-Amino-2-naphthalenepropanoic Acid Phenylmethyl Ester,
4-methylphenylsulfonic Acid Salt
[0800] 356
[0801] A mixture of (S)-2-amino-3-(2-naphthyl)-propanoic acid (0.40
g), benzyl alcohol (2 ml), and p-toluenesulfonic acid monohydrate
(0.42 g) in toluene (5 ml) was heated for 6 hr under reflux.
[0802] The reaction mixture was cooled and diluted with ether (10
ml)/hexane (10 ml). The resulting precipitate was collected by
filtration and recrystallized from EtOH/ether to give
(S)-.alpha.-Amino-2-naphthalen- epropanoic acid phenylmethyl ester,
4-methylphenylsulfonic acid salt (0.73 g), mp 174-176.degree.
C.
R. Example 11
4-[[(2,6-Dichlorophenyl)amino]carbonyl]-L-phenylalanine Methyl
Ester, Hydrochloride Salt
[0803] 357
[0804] A solution of
4-bromo-N-(tert-butoxycarbonyl)-L-phenylalanine (359 mg) in THF was
added to a THF solution of n-BuLi (2.7 ml of 1.6 M solution in
hexane) at -78.degree. C. The mixture was stirred for 2 hr. at room
temperature, quenched with 1N HCl (15 ml), and extracted with
AcOEt. The extract was dried over Na.sub.2SO.sub.4 and the solvent
was removed in vacuo. The residue was purified by column
chromatography on silica gel (eluent: Hexane AcOEt) to give
N-(tert-butoxycarbonyl)-4-[(2,6-
-dichlorophenyl)-carbamoyl]-L-phenylalanine (95 mg). The obtained
compound was treated in a similar manner as described in R. Example
4 to give 4-[[(2,6-Dichlorophenyl)amino]carbonyl]-L-phenylalanine
methyl ester, hydrochloride salt.
[0805] R. Examples 12-46 were prepared in a similar manner as
described in R. Examples 4-11, and are shown in the Tables 6-8.
7TABLE 6 358 R. Examples 12 through 26: R. Ex. No. * R.sup.15
Z.sup.1 R.sup.13 R.sup.18 12 S H single t-BuO-- H bond 13 S H
--OCH.sub.2-- 359 H 14 S H --OCH.sub.2-- 360 H 15 S H --OCH.sub.2--
361 H 16 S H --OCH.sub.2-- 362 H 17 S I --OCH.sub.2-- 363 I 18 S H
--OCH.sub.2-- 364 H 19 S PhCH.sub.2O --OCH.sub.2-- Ph H 20 S H
--OCH.sub.2-- 365 H 21 S H --OCH.sub.2-- Ph H 22 R H --OCH.sub.2--
Ph H 23 S NO.sub.2 --OCH.sub.2-- 366 H 24 S OH --OCH.sub.2-- 367 H
25 S 368 --OCH.sub.2-- 369 H 26 S 370 single Bond OH H
[0806]
8TABLE 7 371 R. Examples 27 through 35: R. Ex. No. Z.sup.2 R.sup.16
27 single bond Ph.sub.3C-- 28 CH.sub.2 372 29 CH.sub.2 Ph 30
CH.sub.2 373 31 CH.sub.2 PhCH.sub.2O 32 CH.sub.2 374 33 CH.sub.2
375 34 CH.sub.2 376 35 CH.sub.2 377
[0807]
9TABLE 8 378 R. Examples 36 through 46: R. Ex. No. * R.sup.6 36 S
379 37 S 380 38 S 381 39 S 382 40 S Ph 41 S 383 42 S 384 43 S 385
44 S 386 45 S 387 46 R 388
R. Example 47
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic Acid 3-methyl
ester (C.sub.11H.sub.18O.sub.4), 36-B
[0808] 389
[0809] Reference Example 47 was prepared according to Scheme 35:
36-B where R.sup.36-1 is CH.sub.3, and the stereochemistry is
(1S-cis) as follows:
[0810] The preparation follows that of Preparation 10-A using
(1S,3R)-(-)-Camphoric acid as the starting material. Physical
properties as follows: .sup.1H NMR (CDCl.sub.3) .delta. 11.45 (1H),
3.69 (3H), 2.82 (1H), 2.54 (1H), 2.24 (1H), 1.82 (1H), 1.52 (1H),
1.27 (3H), 1.25 (3H), 0.86 (3H); Anal: Calcd. for
C.sub.11H.sub.18O.sub.4: C, 61.66; H, 8.47; Found: C, 61.60; H,
8.30.
R. Example 48
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic Acid
1-(1,1-dimethylethyl)-3-methyl Diester (C.sub.15H.sub.26O.sub.4)
36-C
[0811] 390
[0812] Reference Example 48 was prepared according to Scheme 36:
36-C where R.sup.36-1 is CH.sub.3, R.sup.36-2 is t-Bu, and the
stereochemistry is (1S-cis) as follows:
[0813] To a solution of
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxy- lic acid
3-methyl ester (9.69 g), 36-B, in 3 mL of CH.sub.2Cl.sub.2 and 80
mL of cyclohexane was added t-Butyl-2,2,2-trichloroacetimidate
(16.18 mL) and 35 .mu.L of BF.sub.3.Et.sub.2O. The reaction was
stirred at ambient temperature for 18 hours and then filtered. The
filtrate was purified by flash chromatography silica gel eluting
with AcOEt-hexane to obtain
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-3-methyl diester (12.89 g), 36-C. Physical
properties as follows: m.p.=35-37.degree. C.; .sup.1H NMR
(CDCl.sub.3) .delta. 3.68 (3H), 2.79 (1H), 2.53 (1H), 2.17 (1H),
1.76 (1H), 1.24 (3H), 1.17 (3H), 0.81 (3H); Anal: Calcd. for
C.sub.15H.sub.26O.sub.4: C, 66.64; H, 9.67; Found: C, 66.61; H,
9.64.
R. Example 49
(1S-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic Acid
1-(1,1-dimethylethyl) Ester (C.sub.14H.sub.24O.sub.4), 36-D
[0814] 391
[0815] Reference Example 49 was prepared according to Scheme 36 in
the Preparation of 36-D where R.sup.36-2 is t-Bu and the
stereochemistry is (1S-cis) as follows:
[0816] The preparation follows that of Preparation 15-D using 36-C,
(1S-cis)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl)-3-methyl diester, as starting material.
Physical properties as follows: m.p.=95.7-97.5.degree. C.; .sup.1H
NMR (CDCl.sub.3) .delta. 2.82 (1H), 2.53 (1H), 2.13 (1H), 1.82
(1H), 1.45 (10H), 1.29 (3H), 1.17 (3H), 0.88 (3H); Anal: Calcd. for
C.sub.15H.sub.26O.sub.4: C, 65.60; H. 9.44; Found: C, 65.60; H,
9.44.
R. Example 50
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-carbo-
xylic acid 1-(1,1-dimethylethyl) ester (C.sub.21H.sub.30O.sub.4),
36-E-1
[0817] 392
[0818] Reference Example 50 was prepared according to Scheme 36:
Preparation of 36-E-1 where R.sup.36-1 is --CH.sub.2C.sub.6H.sub.5,
R.sup.36-2 is t-Bu, and the stereochemistry is (1S-trans) as
follows:
[0819] To a solution of
(1S-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxy- lic acid
1-(1,1-dimethylethyl) ester 36-D (20.0 g) in 300 mL of CH.sub.3CN
was added benzyl bromide (16.01 g) and DIEA (12.1 g). The reaction
was stirred at ambient temperature for 22 hours, concentrated in
vacuo, and diluted with CH.sub.2Cl.sub.2. The solution was
consecutively washed with water, 10% HCl, water, and brine. The
organic layer was dried (Na.sub.2SO.sub.4) and concentrated in
vacuo. The concentrate was purified by chromatography on silica gel
eluting with AcOEt-hexane to obtain
(1S-cis)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-
-carboxylic acid 1-(1,1-dimethylethyl) ester (18.94 g)
[0820] Physical properties as follows: .sup.1H NMR (CDCl.sub.3)
.delta. 7.34 (5H), 5.12 (2H), 2.82 (1H), 2.51 (1H), 2.19 (1H), 1.82
(1H), 1.44 (10H), 1.23 (3H), 1.15 (3H), 0.78 (3H); MS (EI) m/z
(rel. intensity) 0 (M+, 0), 181 (64), 180 (64), 179 (17), 166 (22),
155 (16), 153 (15), 109 (18), 92 (15), 91 (99), 57 (40); Anal.
Calcd for C.sub.21H.sub.30O.sub.4: C, 72.80; H, 8.73; Found: C,
70.76; H, 8.38; N, 0.36.
[0821] The product of the above reaction was combined with THF (44
mL) and NaH (634 mg) and refluxed for 0.5 hour then cooled, diluted
with saturated NaHCO.sub.3, and extracted with AcOEt. The organic
layer was concentrated to obtain a mixture of starting material and
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-carb-
oxylic acid 1-(1,1-dimethylethyl) ester. These were separated by
HPLC on an (R, R)Whelk-O, 5.times.25 cm column, eluting with 2%
isopropanol in hexane at 50 mL/minute. The detector monitored 215
nm. The first peak at 19 minutes contained
(1S-trans)-[1-(Phenylmethoxy)carbonyl]-1, 2,
2-trimethylcyclopentane-1-carboxylic acid 1-(1,1-dimethylethyl)
ester 36-E-1 (3.88 g). Physical properties as follows: .sup.1H NMR
(CDCl.sub.3) .delta. 7.34 (5H) 5.12 (2H), 3.02 (1H), 2.18 (2H),
1.98 (1H), 1.58 (1H), 1.44 (9H), 1.08 (3H), 1.06 (3H), 0.79 (3H);
MS (FAB) m/z 347 (MH+), 501, 292, 291, 245, 183, 109, 92, 91, 57,
41; HPLC 0.46.times.25 cm (R, R)Whelk-O column eluted at 0.5
mL/minute with 2 isopropanol in hexane, monitoring 215 nm, RT 13.65
minutes.
R. Example 51
(1R-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-carbo-
xylic Acid 1-(1,1-dimethylethyl) Ester (C.sub.21H.sub.30O.sub.4),
36-E-2
[0822] 393
[0823] Reference Example 51 was prepared according to Scheme 36:
Preparation of 36-E-2 where R.sup.36-1 is --CH.sub.2C.sub.6H.sub.5,
R.sup.36-2 is t-Bu, and the stereochemistry is (1R-tranis) as
follows:
[0824] The preparation follows that of Preparation 36-E-1 using
15-D, (1R-cis)-1,2,2-Trimethylcyclopentane-1,3-dicarboxylic acid
1-(1,1-dimethylethyl) ester, as starting material. Physical
properties as follows: .sup.1H NMR (CDCl.sub.3) .delta. 7.35 (5H),
5.12 (2H), 3.02 (1H), 2.13 (2H), 1.98 (1H), 1.58 (1H), 1.44 (9H),
1.08 (3H), 1.05 (3H), 0.78 (3H); HPLC 0.46.times.25 cm (R,
R)Whelk-O column eluted at 1.0 mL/minute with 2 isopropanol in
hexane, monitoring 254 nm, RT=5.54 minutes
R. Example 52
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-carbo-
xylic Acid (C.sub.17H.sub.22O.sub.4), 36-F-1
[0825] 394
[0826] Reference Example 52 was prepared according to Scheme 36:
Preparation of 36-F-1 where R.sup.36-1 is --CH.sub.2C.sub.6H.sub.5,
and the stereochemistry is (1S-trans) as follows:
[0827] A sample of 36-E-1,
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-tr-
imethylcyclopentane-1-carboxylic acid 1-(1,1-dimethylethyl) ester
(2.95 g), was dissolved in TFA at 0.degree. C. and then allowed to
come to ambient temperature. The solution was stirred for 19 hours
then concentrated, redissolved in t-butyl methyl ether and washed
with a saturated solution of NaHCO.sub.3. The organic layer was
concentrated in vacuo to obtain
(1S-trans)-[3-(phenylmethoxy)carbonyl]-1,2,2-trimethylcyc-
lopentane-1-carboxylic acid 36-F-1 (2.44 g).
[0828] Physical properties as follows: .sup.1H NMR (CDCl.sub.3)
.delta. 7.34 (, 5H), 5.13 (, 2H), 3.08 (, 1H), 2.16 (, 2H), 2.02 (,
1H), 1.64 (, 1H), 1.16 (, 3H), 1.10 (, 3H), 0.83 (, 3H); Anal.
Calcd for C.sub.17H.sub.22O.sub.4: C, 70.32; H, 7.64. Found: C,
70.94; H, 7.63; N, 0.04.
R. Example 53
(1R-trans)-[3-(Phenylmethoxy)
carbonyl]-1,2,2-trimethylcyclopentane-1-carb- oxylic Acid
(C.sub.17H.sub.22O.sub.4), 36-F-2
[0829] 395
[0830] Reference Example 53 was prepared according to Scheme 36:
Preparation of 36-F-2 where R.sup.36-1 is --CH.sub.2C.sub.6H.sub.5,
and the stereochemistry is (1R-trans) as follows:
[0831] The preparation follows that of Preparation 36-F-1 using
36-E-2,
(1R-trans)-[3-(Phenylmethoxy)carbonyl]l-1,2,2-trimethylcyclopentane-1-car-
boxylic acid 1-(1, 1-dimethylethyl) ester, as starting material.
Physical properties as follows:
[0832] MS (EI) m/z (rel. intensity) 290 (M.sup.+, 5), 183 (15), 182
(9), 164 (8), 153 (22), 136 (10), 109 (22), 92 (24), 91 (99), 65
(9), 55 (9); Anal. Calcd for C.sub.17H.sub.22O.sub.4: C, 70.32; H,
7.64. Found: C, 70.66; H, 7.74; N, 0.22.
R. Example 54
(1S-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic Acid
1-(1,1-dimethylethyl) Ester (C.sub.14H.sub.24O.sub.4), 36-G-1
[0833] 396
[0834] Reference Example 54 was prepared according to Scheme 36:
Preparation of 36-G-1 where R.sup.36-2 is t-Bu and the
stereochemistry is (1S-trans) as follows:
[0835] A sample of-36-E-1,
(1S-trans)-[3-(Phenylmethoxy)carbonyl]-1,2,2-tr-
imethylcyclopentane-1-carboxylic acid 1-(1,1-dimethylethyl) ester
(3.27 g), was dissolved in EtOH (100 mL) and added 5% palladium on
carbon (1.0 g) and cyclochexene (50 mL). The mixture was refluxed
for 4 hours and then stirred at ambient temperature for 18 hours.
The reaction was then filtered and the filtrate concentrated in
vacuo to obtain 36-G-1,
(1S-trans)-1,2,2-trimethylcyclopentane-1-carboxylic acid
1-(1,1-dimethylethyl) ester (2.45 g). Physical properties as
follows: .sup.1H NMR (CDCl.sub.3) .delta. 3.05 (1H), 2.11 (2H),
1.99 (1H), 1.60 (1H), 1.45 (9H), 1.11 (3H), 1.10 (3H), 0.89 (3H);
MS (FAB) m/z (rel. intensity) 257 (MH.sup.+, 43), 411 (23), 257
(43), 201 (99), 183 (20), 177 (14), 155 (26), 109 (36), 57 (81), 41
(22), 29 (15); Anal. Calcd for C.sub.14H.sub.24O.sub.4: C, 65.60;
H. 9.44. Found: C, 65.62; H, 9.42; N, 0.03.
R. Example 55
(1R-trans)-1,2,2-trimethylcyclopentane-1,3-dicarboxylic Acid
1-(1,1-dimethylethyl) Ester (C.sub.14H.sub.24O.sub.4), 36-G-2
[0836] 397
[0837] Reference Example 55 was prepared according to Scheme 36:
Preparation of 36-G-2 where R.sup.36-2 is t-Bu and the
stereochemistry is (1R-trans) as follows:
[0838] The preparation follows that of Preparation 36-G-1 using
36-E-2,
(1R-trans)[3-(Phenylmethoxy)carbonyl]-1,2,2-trimethylcyclopentane-1-carbo-
xylic acid 1-(1, 1-dimethylethyl) ester, as starting material.
Physical properties as follows: .sup.1H NMR (CDCl.sub.3) .delta.
3.03 (, 1H), 2.13 (, 2H), 1.98 (, 1H), 1.59 (, 1H), 1.45 (, 9H),
1.11 (, 3H), 1.10 (, 3H), 0.89 (, 3H); MS (FAB) m/z (rel.
intensity) 257 (MH+, 35), 411 (19), 279 (14), 257 (35), 201 (99),
183 (18), 155 (26), 1.09 (35), 57 (74), 41 (19), 29 (12); Anal.
Calcd for C.sub.14H.sub.24O.sub.4: C, 65.60; H, 9.44. Found: C,
65.56; H, 9.44; N, 0.18.
R. Example 56
3-Bromo-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine Methyl Ester
Hydrochloride Salt
(C.sub.17H.sub.15BrCl.sub.2N.sub.2O.sub.3.HCl)
[0839] 398
[0840] Reference Example 56 was prepared as follows:
[0841] To a solution of
4-[(2,6-Dichlorobenzoyl)amino]-L-phenylalanine methyl ester
hydrochloride salt, 37-B-1, (205.7 mg) in acetic acid (5 mL) was
added an excess of bromine (5.55 g) in acetic acid (5 mL) and iron
powder (416.2 mg). The reaction was stirred at ambient temperature
for 3 hours. The reaction concentrated in vacuo and the remaining
acetic acid removed as an azeotrope with toluene. The crude
material was diluted with water, made basic with saturated sodium
bicarbonate and extracted with AcOEt. The extract was purified by
flash chromatography on silica gel eluting with methanol-methylene
chloride. The purified material was dissolved in methanol saturated
with hydrogen chloride and concentrated in vacuo and then
crystallized from methanol with AcOEt to obtain
3-Bromo-4-[(2,6-dichlorobenzoyl)amino]-L-phenylalanine methyl ester
hydrochloride salt (540.4 mg). Physical properties as follows: m.p.
200-205; .sup.1H NMR (CD.sub.3OD) .delta. 7.76 (1H), 7.64 (1H),
7.46 (3H), 7.34 (1H), 4.38 (1H), 3.85 (3H), 3.30 (1H), 3.15 (1H);
HRMS (FAB) Calcd for C.sub.17H.sub.15BRCL.sub.2N.sub.2O.sub.3+H,
444.9722, found 444.9724.
R. Example 57
N-[(1,1-Dimethylethoxy)carbonyl]-3-iodo-L-alanine Methyl Ester
(C.sub.9H.sub.16INO.sub.4).
[0842] 399
[0843] The preparation of Reference Example 57 is taught by Scheme
39 under the heading Preparation of Reference Example 57.
R. Example 58
[0844] 400
[0845] The aminoester product of Reference Example 58 is useful as
a synthetic intermediate (for example, reagent 37-B of Scheme 37).
Reference Example 58 is prepared as-follows:
[0846] To a cold (0-5.degree. C.) solution of anhydrous methanolic
HCl was added 100 g of L-4-nitrophenylalanine (Advanced ChemTech)
portionwise over 15 min. The mechanically stirred mixture was
heated to gentle reflux for 48 h. The mixture was allowed to cool
and then filtered through a sintered glass filter funnel, washing
the collected solids with hot MeOH until only insoluble residues
remained. The filtrate was concentrated in vacuo to afford the
methyl ester (120 g) as waxy off white solid which was used without
further purification.
[0847] To a suspension of methyl ester described above (87 g, 0.33
mole) in CH.sub.2Cl.sub.2 (1500 mL) at ambient temperature was
added di-t-butyldicarbonate (109 g, 0.50 mole) followed by the
dropwise addition of Et.sub.3N (51 mL, 0.37 mole). After 15 min
additional Et.sub.3N (40 mL, 0.29 mol) was added to maintain a
slightly basic mixture (ca. pH 8). The reaction mixture was stirred
18 h and additional CH.sub.2Cl.sub.2 (1400 mL) and Et.sub.3N (15
mL, 0.11 mol) were added. After an additional 2 h the reaction
mixture was quenched by the slow addition of MeOH (100 mL), stirred
for 1 h and then partitioned between CH.sub.2Cl.sub.2 and cold 10%
aqueous KHSO.sub.4. The organic layer was washed with saturated
NaHCO.sub.3 and brine, dried (Na.sub.2SO.sub.4), filtered and
concentrated in vacuo. Flash chromatography of the residue using
hexane and a gradient of a 1:1 mixture of
EtOA.sub.c/CH.sub.2Cl.sub- .2 (25-33%) afforded the Boc-methyl
ester (69 g) as a white solid. Physical properties as follows:
.sup.1H NMR (300 MHz; CDCl.sub.3) .delta. 8.16 (2H), 7.31 (2H),
5.04 (1H), 4.63 (1H), 3.73 (3H), 3.18 (2H), 1.41 (9H); MS (ES+) for
C.sub.15H.sub.20N.sub.2O.sub.6 m/z 325.2 (M+H).sup.+.
[0848] Palladium on carbon (100% w/w, 1.25 g) was added to a Parr
hydrogenation flask under a N.sub.2 atmosphere and carefully wetted
with 100 mL of MeOH/THF (1:1). A solution of the Boc-methyl ester
described above (25 g, 77 mmol) in 400 mL of MeOH/THF (1:1) was
added and the mixture shaken on a hydrogenation apparatus under a
hydrogen atmosphere (20 psi) for 1 h at ambient temperature. The
reaction mixture was filtered through a pad of Celite and the
solids washed several times with MeOH. The combined filtrates were
concentrated in vacuo to afford the 4-aminophenylalanyl derivative
(22.7 g) which was used without further purification. Physical
properties as follows: .sup.1H NMR (300 MHz, CDCl.sub.3) .delta.
6.89 (2H), 6.61 (2H), 4.96 (1H), 4.50 (1H), 3.69 (3H), 2.95 (2H),
1.41 (9H); MS (ES+) for C.sub.15H.sub.22N.sub.2O.sub.4 m/z 295.2
(M+H).sup.+.
[0849] A cold (0-5.degree. C.) solution of 2,6-dichlorobenzoyl
chloride (11.1 mL, 77.5 mmol) in 125 mL of THF was treated dropwise
with a solution of the 4-aminophenylalanyl derivative described
above (22.7 g, 77.1 mmol) and Et.sub.3N (16 mL, 115 mmol) in 125 mL
of THF. The reaction mixture was allowed to warm to temperature and
stir an additional 18 h. The mixture was diluted with EtOAc (2 L)
and then washed with 1N HCl, H.sub.2O, 1N NaOH and brine. The
organic extract was dried (Na.sub.2SO.sub.4), filtered, and
concentrated in vacuo to give the crude product as a pale yellow
solid. This material was recrystallized from acetone/hexanes (ca.
1:1) to afford the amide (30.8 g) as a crystalline solid. Physical
properties as follows: mp 192.2-193.1.degree. C.; IR (mull) 3305,
1747, 1736, 1690, 1665, 1609, 1548, 1512, 1433, 1414, 1325, 1277,
1219, 1171, 781 cm.sup.-1; .sup.1H NMR (300 MHz; CDCl.sub.3)
.delta. 7.57 (2H), 7.34 (4H) 7.14 (2H), 4.98 (1H), 4.60 (1H), 3.74
(3H), 3.11 (2H), 1.42 (9H); MS (ES+) for
C.sub.22H.sub.24Cl.sub.2N.sub.2O.sub.5 m/z 467.0 (M+H).sup.+.
[0850] To 650 mL of anhydrous 4M HCl in dioxane at ambient
temperature was added the amide described above (30.6 g, 65.5 mmol)
portionwise and the resulting mixture was stirred until all the
solids had dissolved (ca. 1 h). Volatiles were removed in vacuo to
give a light yellow solid which was partitioned between water (500
mL) and ether (1 L). The water layer was separated and concentrated
in vacuo to approximately 200 mL. The aqueous solution was then
frozen and lyophilized to afford the aminoester product Reference
Example 58 (25.6 g) as a light yellow solid. Physical properties as
follows: [.alpha.].sup.25.sub.D=+5 (c 1, MeOH); IR (mull) 3244,
3186, 3112, 1747, 1660, 1604, 1562, 1539, 1516, 1431, 1416, 1327,
1273, 1243, 799 cm.sup.-1; .sup.1H NMR (300 MHz; CD.sub.3OD)
.delta. 7.69 (2H) 7.45 (3H), 7.29 (2H), 4.34 (1H), 3.83 (3H), 3.21
(2H); .sup.13C NMR (300 MHz; CD.sub.3OD) .delta. 169.0, 163.9,
137.8, 136.08, 131.8, 131.0, 130.3, 129.7, 127.9, 120.6, 53.8,
52.3, 35.4; MS (ES+) for C.sub.17H.sub.16Cl.sub.2N.sub.2O.sub.3 m/z
367.1 (M+H).sup.+.
[0851] Biological Assays
[0852] Jurkat-Endothelial Cell Adhesion Assay:
[0853] The following assay established the activity of the present
compounds in inhibiting .beta..sub.1-mediated cell adhesion in a
representative in vitro system. This assay measures the adhesive
interactions of a T-cell line, Jurkat, known to express the
.alpha..sub.4.beta..sub.1 integrin, to endothelial monolayers in
the presence of test compounds. The test compounds were added in
increasing concentrations to T-cells and then the T-cell compound
mixture was added to IL-1 stimulated endothelial cell monolayers.
The plates were incubated, washed and the percentage of attached
cells was quantitated. The present assay directly demonstrates the
cell adhesion inhibitory activity and adhesion modulatory activity
of the compounds. Human umbilical vein endothelial cells were
purchased from Clonetics (San Diego, Calif.) at passage number 2.
The cells were grown on 0.5% porcine skin gelatin pre-coated flasks
(Sigma, St. Louis Mo.) in EGM-UV media (Clonetics, San Diego,
Calif.) supplemented with 10% fetal bovine serum. Cells are refed
every 2-3 days reaching confluence by day 4 to 6. The cells are
monitored for factor VIII antigen and results show that at passage
12, the cells are positive for this antigen. The endothelial cells
are not used following passage 6. The T-cell line Jurkat was
obtained from American Type Tissue Culture Collection (Rockville,
Md.) and the cells were cultured in RPMI containing 10% fetal calf
serum. The cells were washed twice in Hank's Balanced Salt Solution
(HBSS) and resuspended in Dulbecco's Minimal Eagle's Media (DMEM)
containing 2.5 mg/ml Human Serum Albumin (HSA). Jurkat cells
(1.times.10.sup.6 cells/ml) were stained with 10 ng/ml BCECF-AM
(Molecular Probes, Eugene, Oreg.)) in HESS without phenol red. The
cells were loaded with BCECF for 60 minutes in the dark at
37.degree. C., washed 2 times, and resusupended in DMEM-HSA--
solution.
[0854] Confluent endothelial monolayers, grown in 96-well tissue
culture plates, were stimulated for 4 hr. at 37.degree. C. with 0.1
ng/ml (.about.50 U/ml) recombinant IL-1 (Amgen, Thousand Oaks,
Calif.). Following this incubation, the monolayers were washed
twice with HESS and 0.1 ml of DMEM-HSA solution was added. Jurkat
cells (5.times.10.sup.5 cells) were combined with the appropriate
concentration of the test compound and 0.1 ml of the Jurkat
cell-compound mixture was added to the endothelial cell monolayers.
Generally, 100, 20, 5 and 1.25 .mu.M compound concentrations were
tested. These concentrations are adjusted downward for analogs
found or thought to be more potent. The plates were placed on ice
for 5 minutes to allow for Jurkat cell settling and the plates were
incubated at 37.degree. C. for 20 minutes. Following this
incubation, the monolayers were washed twice with PBS containing 1
mM calcium chloride and 1 mM magnesium chloride and the plates were
read using a Millipore Cytofluor 2300 (Marlboro, Mass.).
Fluorescence in each well was measured as Arbitrary Fluorescence
Units and percent adhesion in the absence of compound was adjusted
to 100% and the % adhesion in the presence of compound was
calculated. Monolayers were also fixed in 3% paraformaldehyde and
evaluated microscopically to verify the adhesion. This procedure is
a modification of a previously published method (Cardarelli et al.,
J. Diol. Cheem. 269:18668-18673 (1994)).
[0855] Jurkat-CS-1 Assay
[0856] The CS-1 derived peptide, CLHPGEILDVPST, and the scrambled
control peptide, CLHGPIELVSDPT, were synthesized on a Beckman 990
synthesizer using t-Boc methodology. The peptides were immobilized
onto microtiter plates using the heterobifunctional crosslinker
3-(2-pyridyldithio)propio- nic acid N-hydroxysuccinimide ester
(SPDP) as reported by Pierschbacher et al., Proc. Natl. Acad. USA,
801224-1227 (1983). Microtiter plates were coated with 20 .mu.g/ml
HSA for 2 hr. at room temperature, washed once with PBS and
derivatized with 10 .mu.g/ml SPDP for 1 hr. After washing, 100
.mu.l of a 100 .mu.g/ml cysteine containing-peptide solution which
had been recently dissolved was added to the wells and allowed to
crosslink to the plates overnight at 4.degree. C. Unbound peptide
was removed from plates by washing with PBS. To block non-reacted
sites, the plates are coated with 100 .mu.l of a 2.5 mg/ml BSA
solution in PBS for 1 hr. at 37.degree. C. 100 .mu.l of Jurkat
cells (2.5.times.10.sup.6 cells/ml) in DMEM plus BSA (2.5 mg/ml)
was mixed with an appropriate concentration of the compound to be
tested and the mixture was added to peptide coated dishes and
incubated for 1 hr. at 37.degree. C. Generally 100, 20, 5 and 1.25
.mu.M concentrations of the compound were tested. The
concentrations of the compound were adjusted downward for compounds
thought or found to be more potent.
[0857] Following this incubation the plates were washed once with
PBS and the attached cells were fixed with 3% paraformaldehyde in
PBS and stained with 0.56 toluidine blue in 3.7% formaldehyde. The
cells were stained overnight at room temperature and the optical
density at 590 nm of toluidine blue stained cells was determined
using a vertical pathway spectrophotometer to quantitate attachment
(VMAX Kinetic Microplate Reader, Molecular Devices, Menlo Park,
Calif.). This procedure is-a modification of a previously published
method (Cardarelli et al, J. Biol. Chem., 269:18668-18673 (1994)
and Cardarelli et al, Proc. Natl. Acad. Sci. USA, 83:2647-2651
(1986)).
[0858] The preferred compounds are those which have low IC.sub.50
values in the Jurkat EC assay or the Jurkat-CS-1 assay described
above or which have at least moderate activity in both assays. All
of the compounds of the present invention have an activity of less
than 50 .mu.M in the Jurkat CS-1 assay or less than 500 .mu.M in
the Jurkat EC assay. Compounds with activity in the Jurkat CS-1
assay preferably have IC.sub.50 values of less than 1 .mu.M, more
preferably less than 0.5 .mu.M, most preferably less than or equal
to 0.08 .mu.M. Compounds with activity in the Jurkat EC assay
preferably have IC.sub.50 values of less than 10 .mu.M, more
preferably less than 5 .mu.M, most preferably less than or equal to
0.8 .mu.M.
[0859] In the Jurkat EC Assay, IC.sub.50 value ranges (.mu.M) are
depicted by A, B, and C and in the Jurkat CS-1 Assay, IC.sub.50
value ranges are depicted by D, E, and F. These ranges are as
follows:
[0860] Jurkat EC: 5.ltoreq.A<10, 0.8<B<5, and
C.ltoreq.0.8
[0861] Jurkat CS-1: 0.5.ltoreq.D<1, 0.08<E<0.5, and
F.ltoreq.0.08
[0862] The following chart illustrates the IC.sub.50 values for
selected compounds of the present invention in the Jurkat EC Assay
and the Jurkat CS-1 Assay, The ranges are so described above.
10 ADDITIONAL IN VITRO BIOLOGICAL DATA Example No. Jurkat EC Jurkat
CS-1 2 -- D 4 B E 10 B E 12 C F 13 A D 14 A -- 16 A -- 24 B E 26 B
D 28 B E 29 A D 31 A -- 36 A D 38 B E 46 B -- 53 B D 54 C F 61 B E
62 A -- 63 B F 65 C E 75 B -- 77 B E 79 A -- 81 C E 83 C F 85 A E
86 -- D 87 C F 89 B E 91 B F 92 C F 93 C F 95 B E 96 A -- 97 C F
100 C F 102 C F 103 C F 104 C F 105 B E 106 C F 108 C F 110 C F 112
C F 113 -- D 114 C E 116 C F 118 B D 120 B E 121 B D 122 B -- 124 B
E 126 B E 128 B E 130 B E 132 B E 134 B -- 136 A D 137 -- E 141 B D
142 B E 143 -- D 144 B E 146 B E 148 C E 150 B D 152 C F 153 B E
155 A D 161 B -- 163 -- D 166 C F 170 A -- 179 A F 180 C F 181 C F
182 C F 183 C F 184 C F 185 B E 186 B E 187 B E 188 B E 189 B --
194 B E 208 C E 209 C F 210 C F 211 C F 212 A E 213 B E 214 C F 215
C E 216 C F 217 C F 219 C F 220 B E 221 A D 222 C E 223 C F 225 C F
231 C D 232 C F 236 C E 253 B E 254 B E
[0863] Activity of Camphoric Acids in Dextran Pleurisy Model
[0864] Certain compounds of the present invention were tested in a
Dextran.RTM. pleurisy model.
[0865] Rationale for Developing an .alpha..sub.4.beta..sub.1
Integrin Antagonist to Treat Inflammatory Diseases
[0866] VLA-4, a member of the .beta.1 integrin family of adhesion
molecules, is thought to play a critical role in several types of
inflammatory disease processes by promoting leukocyte adhesion to
vascular cell adhesion molecule (VCAM-1) and the CS-1 domain of
fibronectin in extracellular tissue matrix (Elices M J, Osborn L,
Takada Y, Crouse C, Luhowskyj S, Hemler M, Lobb R R. VCAM-1 on
activated endothelium interacts with the leukocyte integrin VLA-4
at a site distinct from the VLA-4-fibronectin binding site. Cell;
60: 577-584, 1990, Humphries M J, Akiyama S K, Komoriya A, Olden K,
Yamada K M. Identification of an alternatively-spliced site in
human plasma fibronectin that mediates cell type-specific adhesion.
J Cell Biol; 103: 2637-2647, 1986, Wayner E A, Garcia-Pardo A,
Humphries M J, McDonald J A, Carter W G. Identification and
characterization of the T lymphocyte adhesion receptor for an
alternative cell attachment domain (CS-1) in plasma fibronectin. J
Cell Biol; 109: 1321-1330, 1989, Guan J-L, Hynes R O. Lymlphoid
cells recognize an alternatively-sipliced segment of fibronectin
via the integrin .alpha..sub.4.beta..sub.1. Cell; 60: 53-61 1990)
Of the cell types expressing VLA-4, the major emphasis has been on
eosinophils, lymphocytes, and monocytes. Validation of the role of
VLA-4 has relied predominantly on the use of anti-VLA-4 antibodies
which have been shown to suppress delayed-type hypersensitivity
responses (Issekutz T B. Dual inhibition of VLA-4 and LFA-1
maximally inhibits cutaneous delaved-type hypersensitivity-induced
inflammation. Am J Pathol; 143: 1286-1293, 1993, Scheynius A, Camp
R L, Pur E. Reduced contact sensitivity reactions in mice treated
with monoclonal antibodies to leukocyte function-associated
molecule-1 and intercellular adhesion molecule-1. J Immunol; 150:
655-663, 1993, Ferguson T A, Kupper T S. Antigen-independent
processes in antigen-specific immunity. J Immunol; 150: 1172-1182,
1993, Chisholm P L, Williams C A, Lobb R R. Monoclonal antibodies
to the integrin .alpha.-4 subunit inhibit the murine contact
hypersensitivity response. Eur J Immunol; 23: 682-688, 1993, Elices
M J, Tamraz S, Tollefson V, Vollger L W. The integrin VLA-4
mediates leukocyte recruitment to skin inflammatory sites in vivo.
Clin Exp Rheumatol; 11 (Suppl 8) S77-80), 1993, experimental
allergic encephalomyelitis (Yednock T A, Cannon C, Fritz L C,
Sanchez-Madrid F, Steinman L M, Karin N. Prevention of experimental
autoimmune encephalomyelitis by antibodies against
.alpha..sub.4.beta..sub.1 integrin. Nature; 356: 63-66, 1992,
Canella B, Raine C S. The VCAM-1/VLA-4 pathway is involved in
chronic lesion expression in multiple sclerosis (MS). J Neuropathol
Exp Neurol; 52: 311, 1993), HIV-induced encephalitis (Sasseville V
G, Newman W, Brodie S J, Hesterberg P, Pauley D, Ringler D J.
Monocyte adhesion to endothelium in simian immunodeficiency
virus-induced AIDS encephalitis is mediated by vascular cell
adhesion molecule-1/.alpha..sub.4.beta..sub.1 integrin reactions.
Am J Pathol; 144: 27-40, 1994), pulmonary inflammation and airway
hyperreactvity in asthma (Abraham W M, Sielczak M W, Ahmed A,
Cortes A, Lauredo I T, Kim J. Pepinsky, B, et al.
.alpha..sub.1-integrins mediate antigen-induced late bronchial
responses and prolonged airway hyperresponsiveness in sheep. J Clin
Invest; 93: 776-787, 1994, Pretolani M, Ruffi C, Roberto LapaeSilva
J. Joseph D, Lobb R R, Vargaftig B B. Antibody to very late
activation antigen 4 prevents antigen-induced bronchial
hyperreactivity and cellular infiltration in the guinea-pig
airways. J Exp Med; 180: 795-805, 1994), experimental models of
autoimmune-mediated diabetes (Yang X-D, Karin N, Tisch R, Steinman
L, McDevitt H O. Inhibition of insulitis and prevention of diabetes
in non-obese diabetic mice by blocking L-selectin and very late
antigen 4 adhesion receptors. Proc Natl Acad Sci USA; 90:
10494-10498, 1993, Burkly L C, Jakubowski A, Hattori M. Protection
against adoptive transfer of autoimmune diabetes medicated through
very late antigen-4 integrin. Diabetes; 43: 529-534, 1994), and
experimental colitis (Podolsky D K, Lobb R, King N, Benjamin C D,
Pepinsky B, Sehgal P, et al. Attentuation of colitis in the
cotton-top Tamarin by anti-.alpha.4 integrin monoclonal antibody. J
Clin Invest; 92: 372-380, 1993). Since eosinophils represent a
major component of the inflammatory cell influx in asthmatic lung
tissue we developed a simple acute inflammatory model of VLA-4
integrin-dependent eosinophil infiltration which could be used to
identify VLA-4 antagonists; such compounds would be of potential
value in the treatment of asthma as well as other diseases in which
VLA-4 played a role.
[0867] Materials and Methods
[0868] Animals, Housing and Viral Testing:
[0869] C57BL/6 mice (Jackson, Bar Harbor, Me.;), 6-8 weeks old,
weighing 20-25 g were used throughout All mice were acclimated for
at least 7-14 days after arrival and maintained under controlled
temperature (20-22.degree. C.) and a 12 hr daily light cycle (6.00
A.M.-6.00 P.M.). Mice were housed in laminar flow racks and checked
biweekly for viral infections (mouse hepatitis virus, minute virus
of mice, rodent orphan parvovirus, Sendai) with kits obtained from
Oreganon Teknika (Durham, N.C.) using established enzyme-linked
immunoabsorbent assays. Mice testing positive for any of the above
were omitted from the study. All mice were fed standard laboratory
chow (Upjohn Lab Rodent Irradiated Mouse Chow, #5011-3, PMI Feeds,
St. Louis, Mo.) and acidified drinking water (pH 5.0) ad
libitum.
[0870] Induction of Inflammation by Intrapleural Injection of
Dextran:
[0871] Intrapleural injections were made using a 27G needle cut to
3-4 mm and blunted by filing. Injections were made by inserting the
needle between the mid-intercostal ribs on the right side of the
thoracic cavity.
[0872] Dextran (MW 5-40.times.10.sup.6, St Louis, Mo.) was injected
as a 10% solution in saline in a volume of 100 .mu.l/mouse. Care
was taken to avoid bleeding at the site of injection at which the
intercostal muscles were cut to facilitate smooth insertion of the
needle.
[0873] Quantitation of Pleural Inflammatory Leukocyte
Responses:
[0874] Pleural leukocytes were collected as follows: 4 h
post-induction, pleural inflammatory exudate was removed by washing
with 2.times.1.0 ml Ca.sup.++/Mg.sup.++ free HBSS (Gibco, Grand
Island, N.Y.) containing 45 mg EDTA/100 ml HBSS, 4.degree. C. Total
leukocyte counts were made by hemocytometer following erythrocyte
lysis in 2% acetic acid in PBS buffer; exudate leukocyte pellets
were resuspended in serum for cytospin preparations and stained
(Diff Quik, Baxter Healthcare, McGraw Park, Ill.) for differential
leukocyte counts (neutrophils, eosinophils, and mononuclear
leukocytes). The pleural cavities of mice receiving either no
intrapleural injection, or saline were washed and the cells counted
in the same way to estimate baseline or saline-induced pleural
leukocyte counts respectively
[0875] Administration of Drugs:
[0876] All drugs were dissolved in PBS and the pH adjusted to 7.5
with NaOH. Each drug was administered intravenously through the
retroorbital sinus at hourly intervals (0-3 h) starting from time
"0" as indicated. Mice were carefully monitored for side effects;
none were noted for the series of compounds reported herein.
[0877] The following camphoric acid analogues were tested for their
inhibitory effects on dextran-induced leukocyte infiltration:
Examples 4, 12, 54, 63, 166, 93, 180, 181, 183, 184, 217, Camphoric
acid or PBS (saline) was administered iv. as a control. Inhibition
of eosinophll infiltration, which was suppressed by anti-alpha-4
Mab (PS/2, 50%), was used as a readout of VLA-4 antagonist activity
of the camphoric acids tested. Data for neutrophils are also
reported.
[0878] Results:
[0879] Dextran Pleural Leukocyte Response.
[0880] The total pleural leukocyte counts were 255.times.10.sup.4
(+/-16 SEM) cells in the normal pleural cavity; of the normal
pleural leukocyte population, all cells were mononuclear (a similar
response was observed following intrapleural saline injection).
Four hours after intrapleural injection of dextran total pleural
leukocyte counts increased to 719.times.10.sup.4 (+/-67 SEM) and
comprised 36.8.times.10.sup.4 (+/-4.1 SEM) eosinophils,
292.times.10.sup.4 (+/-25 SEM) neutrophils and 391.times.10.sup.4
(+/-48 SEM) mononuclear leukocytes.
[0881] Inhibition of neutrophils and eosinophil (%.DELTA.) are
depicted by A, B and C according to the following ranges:
A.ltoreq.-50, -50<B.ltoreq.-10, -10<C.ltoreq.0
[0882] Inhibition of Dextran Pleural Leukocyte Infiltration by
Camphoric Acids.
11 %.DELTA. Pleural Leukocytes Treatment Dose (mg/kg) # Neutro Eos
Ex. 12 50 .times. 4 iv B A 50 .times. 2 iv B A Ex. 54 50 .times. 4
iv B A Ex. 4 25 .times. 2 iv -- -- 50 .times. 2 iv B A Ex. 63 25
.times. 2 iv B B 50 .times. 2 iv B B Ex. 166 25 .times. 2 iv -- --
50 .times. 2 iv C B Ex. 93 25 .times. 2 iv -- -- 50 .times. 2 iv C
B Ex. 181 25 .times. 2 iv B B 50 .times. 2 iv B B Ex. 180 25
.times. 2 iv B B 50 .times. 2 iv B B Ex. 183 25 .times. 2 iv B B 50
.times. 2 iv B B Ex. 184 25 .times. 2 iv B B 50 .times. 2 iv B A
Ex. 217 25 .times. 2 iv -- -- 50 .times. 2 iv C B # Compounds given
at 0h, +1h (.times.2 iv) or 0h, +1h, +2h, +3h (.times.4 iv)
relative to dextran.
[0883] p<0.05 * Drug treated vs camphoric acid or PBS treated
control.
* * * * *