U.S. patent application number 10/290494 was filed with the patent office on 2003-05-15 for reducing electrolyzed water and method for producing same.
This patent application is currently assigned to Miz Co., Ltd.. Invention is credited to Han, Shouka, Naitou, Tatsuya, Satoh, Fumitake, Yanagihara, Tomoyuki.
Application Number | 20030089618 10/290494 |
Document ID | / |
Family ID | 22015882 |
Filed Date | 2003-05-15 |
United States Patent
Application |
20030089618 |
Kind Code |
A1 |
Satoh, Fumitake ; et
al. |
May 15, 2003 |
Reducing electrolyzed water and method for producing same
Abstract
The present invention provides reducing electrolyzed water which
has a pH of 3 to 12 and an oxidation-reduction potential of up to
-200 mV, preferably a pH of 5 to 11 and an oxidation-reduction
potential of up to -500 mV, and in which the common logarithm of
the product of the hydrogen ion concentration [H.sup.+] and the
electron concentration [e.sup.-] is at least -4.5, preferably at
least 0. The reducing electrolyzed water is used as potable water,
agricultural fertilizers, drip solutions and other injections,
dialysis solutions and face lotion, and particularly shows
significant medical effects.
Inventors: |
Satoh, Fumitake;
(Fujisawa-shi, JP) ; Han, Shouka; (Fujisawa-shi,
JP) ; Yanagihara, Tomoyuki; (Yokosuka-shi, JP)
; Naitou, Tatsuya; (Chigasaki-shi, JP) |
Correspondence
Address: |
ARENT FOX KINTNER PLOTKIN & KAHN
1050 CONNECTICUT AVENUE, N.W.
SUITE 400
WASHINGTON
DC
20036
US
|
Assignee: |
Miz Co., Ltd.
|
Family ID: |
22015882 |
Appl. No.: |
10/290494 |
Filed: |
November 8, 2002 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
10290494 |
Nov 8, 2002 |
|
|
|
09058289 |
Apr 10, 1998 |
|
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Current U.S.
Class: |
205/466 |
Current CPC
Class: |
C02F 1/70 20130101; C02F
2209/06 20130101; C02F 2201/46115 20130101; A61K 33/00 20130101;
C02F 2209/04 20130101; C02F 2001/4619 20130101; C02F 2001/46195
20130101; C02F 1/66 20130101; C02F 1/4618 20130101; C02F 2103/026
20130101; C05G 5/20 20200201 |
Class at
Publication: |
205/466 |
International
Class: |
C25B 001/30 |
Claims
What is claimed is:
1. Reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
2. The Reducing electrolyzed water according to claim 1, wherein
the common logarithm of the product of the hydrogen ion
concentration [H.sup.+] and the electron concentration [e.sup.-] is
at least -4.5.
3. A method for producing reducing electrolyzed water comprising a
step of: electrolyzing subject water containing a reducing agent to
give reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
4. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water and adding a reducing
agent to give reducing electrolyzed water having a pH of 3 to 12
and an oxidation-reduction potential of up to -200 mV.
5. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and adding further a reducing agent to give the reducing
electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
6. A method for producing reducing electrolyzed water comprising a
step of: electrolyzing subject water containing a reducing agent
and metal ions to give the reducing electrolyzed water having a pH
of 3 to 12 and an oxidation-reduction potential of up to -200
mV.
7. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and metal ions and adding further metal ions to give the
reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
8. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water and adding a reducing
agent and metal ions to give the reducing electrolyzed water having
a pH of 3 to 12 and an oxidation-reduction potential of up to -200
mV.
9. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and metal ions and adding further a reducing agent to give
the reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
10. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and adding further a reducing agent and metal ions to give
the reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
11. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and metal ions and adding further a reducing agent and metal
ions to give the reducing electrolyzed water having a pH of 3 to 12
and an oxidation-reduction potential of up to -200 mV.
12. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and metal ions to produce electrolyzed alkaline water having
a pH of 9 to 12 and an oxidation-reduction potential of up to -600
mV, and adding a reducing agent to the electrolyzed alkaline water
to give the reducing electrolyzed water having a pH of 3 to 12 and
an oxidation-reduction potential of up to -200 mV.
13. A method for producing reducing electrolyzed water comprising
the steps of: electrolyzing subject water containing a reducing
agent and metal ions to produce electrolyzed alkaline water having
a pH of 9 to 12 and an oxidation-reduction potential of up to -600
mV, and electrolyzing further the electrolyzed alkaline water to
give the reducing electrolyzed water having a pH of 3 to 12 and an
oxidation-reduction potential of up to -200 mV.
14. A method for producing reducing electrolyzed water according
any one of claims 3 to 13, wherein the common logarithm of the
product of the hydrogen ion concentration [H.sup.+] and the
electron concentration [e.sup.-] of the reducing electrolyzed water
is at least -4.5.
15. A method for producing the reducing electrolyzed water
according any one of claims 3 to 13, wherein the reducing agent is
a mixture having a gamma-lactone structure and containing an OH
group or a saccharide having a five- or six-membered ring and at
least one OH group.
16. A method for producing the reducing electrolyzed water
according any one of claims 3 to 13, wherein the metal ions are
sodium ions, potassium ions, calcium ions or magnesium ions.
wherein the metal ions are sodium ions, potassium ions, calcium
ions or magnesium ions.
17. Reducing electrolyzed water obtained by electrolyzing water
containing a reducing agent and metal ions, keeping the dissolved
oxygen content in a trace amount and maintaining the
oxidation-reduction potential at a low level.
18. Reducing electrolyzed water which comes to have an
oxidation-reduction potential of up to -200 mV when an aqueous
hydroxide solution is added.
19. Potable water comprising the reducing electrolyzed water
according to any one of claims 1, 2, 17 and 18 as the principal
component thereof.
20. An agricultural fertilizer comprising the reducing electrolyzed
water according to any one of claims 1, 2, 17 and 18 as the
principal component thereof.
21. An injection comprising the reducing electrolyzed water
according to any one of claims 1, 2, 17 and 18.
22. A drip solution comprising the reducing electrolyzed water
according to any one of claims 1, 2, 17 and 18.
23. A dialysis solution comprising the reducing electrolyzed water
according to any one of claims 1, 2, 17 and 18.
24. Face lotion comprising the reducing electrolyzed cording to any
one of claims 1, 2, 17 and 18.
Description
BACKGROUND OF THE INVENTION
[0001] 1. Field of the Invention
[0002] The present invention relates to reducing electrolyzed water
obtained by electrolyzing subject water, and potable water,
agricultural fertilizers, drip solutions and other injections,
dialysis solutions and face lotion which contain the reducing
electrolyzed water as their main component, and a method for
producing these materials.
[0003] 2. Description of the Related Art
[0004] It has heretofore been reported that use of electrolyzed
alkaline water derived by electrolyzing subject water produces the
medical effects that extraordinary zymosis and indigestion in the
stomach and intestines, diarrhea and gastric hyperacidity are
suppressed. The medical effects have been considered to be produced
principally by such mineral components contained in electrolyzed
alkaline water and present as cations as calcium, sodium, magnesium
and potassium.
[0005] Electrolyzed alkaline water used for obtaining such medical
effects is exclusively defined only by metal ions contained therein
and the pH, and is produced by electrolyzing subject water to which
calcium, and the like are added until the pH reaches about 9.
[0006] However, as a result of carrying out investigations, the
present inventors have found that disease is mainly caused by the
damage of biomolecules within an organism resulting from oxidation
of the biomolecules with active oxygen formed therewithin.
SUMMARY OF THE INVENTION
[0007] The present inventors have paid attention to that such
active oxygen can be reduced with hydrogen to form non-toxic water,
and found that electrolytic water having higher medical effects can
be obtained if the reaction can be promoted. The present invention
has thus been achieved.
[0008] Furthermore, since such electrolyzed water has been
incapable of being produced by conventional methods, the present
inventors have intensively carried out research while paying
attention to the reduction reaction rate. As a result, they have
discovered that reducing electrolyzed water can be easily formed by
selecting and suitably adding some reducing agents and some metal
ions, and achieved the present invention.
[0009] That is, an object of the present invention is to provide
harmless water excellent in reducing capability and a method for
producing the same.
[0010] In order to achieve the object as mentioned above, the
reducing electrolyzed water of the present invention has a pH of 3
to 12 and an oxidation-reduction potential of up to -200 mV,
preferably a pH of 5 to 11 and an oxidation-reduction potential of
up to -500 mV.
[0011] In the present invention, the common logarithm of the
product of the hydrogen ion concentration [H.sup.+] and the
electron concentration [e.sup.-] is preferably at least -4.5, more
preferably at least 0.
[0012] As a result of supporting a life by conducting metabolism
which utilizes oxygen, an organism forms active oxygen therewithin.
The active oxygen has extremely strong oxidizing capability, and
oxidizes genes and cells forming the organism. The oxidation is
considered to be one cause of disease. The reducing electrolyzed
water of the present invention has a significantly high extinction
activity for active oxygen, and acts to stabilize active oxygen by
accepting active oxygen within the organism.
[0013] Furthermore, the reducing electrolyzed water of the present
invention can maintain its characteristics for a long period of
time, and is excellent in preservability. That is, since the
reducing electrolyzed water of the present invention obtained by
electrolyzing water containing a reducing agent and/or metal ions
contains a reducing agent, the reducing capability of the reducing
agent makes the reducing electrolyzed water preserve dissolved
oxygen in a trace amount even when the water is exposed to an
oxygen environment. Moreover, the reducing electrolyzed water can
maintain its oxidation-reduction potential which has become low at
a low one for a long period of time, by the action of the metal
ions. Accordingly, the reducing electrolyzed water is excellent in
preservability.
[0014] Furthermore, since the reducing electrolyzed water of the
present invention has a pH of 3 to 12, preferably a pH of 5 to 11,
namely a large hydrogen ion concentration in spite of its markedly
low oxidation-reduction potential, it has strong reducing
capability, and has the property that it tends to react with active
oxygen within an organism.
[0015] Active oxygen O.sub.2.sup.- is reduced by the following
reactions to form water:
O.sub.2.sup.-+(H.sup.++e.sup.-).fwdarw.HO.sub.2.sup.-
HO.sub.2.sup.-+H.sup.+.fwdarw.H.sub.2O.sub.2
H.sub.2O.sub.2.fwdarw.2(.OH)
.OH+(H.sup.++e.sup.-).fwdarw.H.sub.2O
[0016] Accordingly, the chemical equilibria are moved more toward
the formation of water H.sub.2O when the hydrogen ion concentration
[H.sup.+] and the electron concentration [e.sup.-] are each larger
and the product of both concentrations are larger. The hydrogen ion
concentration and the electron concentration are expressed as
follows:
hydrogen ion concentration [H.sup.+]=10.sup.-pH,
[0017] and
electron concentration [e.sup.-]=10(.sup.-ORP/0.05917).
[0018] It can, therefore, be said that when the hydrogen ion
concentration [H.sup.+] and the electron concentration [e.sup.-]
are each larger and the product of both concentrations are larger,
that is, when the common logarithm of the product is larger, the
active oxygen is converted into water more. Since the reducing
electrolyzed water of the present invention shows the common
logarithm of the product of a hydrogen ion concentration [H.sup.+]
and an electron concentration [e.sup.-] of at least -4.5,
preferably at least 0, it can decrease the active oxygen
content.
[0019] It is most preferred in the present invention that the
dissolved oxygen content be as close to 0 ppm as possible and that
the oxidation-reduction potential be as low as possible because
inhibition of oxidation by active oxygen within an organism as
mentioned above can be expected most.
[0020] For reasons as described below, the reducing electrolyzed
water of the present invention having such properties is
particularly preferably used as the main component of potable
water, alcoholic beverages, soft drinks, fruit beverages, fermented
lactic drinks, etc., or as drip solutions or other injections,
dialysis solutions and toilet water. Use of the reducing
electrolyzed water is expected to produce the medical effects that
the content of active oxygen which is a byproduct of metabolism
utilizing oxygen within an organism can be decreased, and that
oxidation of genes and cells can be inhibited.
[0021] Furthermore, the reducing electrolyzed water of the present
invention is preferably used for agricultural chemicals and
agricultural fertilizers other than potable water and injections.
Since nitrate nitrogen is used for conventional agricultural
fertilizers, field crops contain a large amount of nitrous acid.
Since vitamin C decreases in the field crops in inverse proportion
to an increase in nitrous acid, the field crops have poor reducing
capability. Moreover, when nitrous acid is taken in an organism, it
combines with an amine to form a harmful amine nitrite
(nitrosoamine). Accordingly, use of the reducing electrolyzed water
of the present invention for agricultural chemicals and
agricultural fertilizers prevents a decrease in the reducing
capability and formation of harmful materials.
[0022] The reducing electrolyzed water of the present invention can
be produced by electrolyzing water containing a reducing agent.
Moreover, it can be produced by electrolyzing water and then adding
a reducing agent. Furthermore, it can be produced by electrolyzing
water containing a reducing agent, and adding further a reducing
agent. Still furthermore, it can be produced by electrolyzing water
containing a reducing agent and metal ions. Moreover, it can be
produced by electrolyzing water containing a reducing agent and
metal ions and adding further metal ions. Furthermore, it can be
produced by electrolyzing water and then adding a reducing agent
and metal ions. Moreover, it can be produced by electrolyzing water
containing a reducing agent and metal ions, and adding further a
reducing agent. Furthermore, it can be produced by electrolyzing
water containing a reducing agent and adding further a reducing
agent and metal ions. Still furthermore, it can be produced by
electrolyzing water containing a reducing agent and metal ions and
then adding further a reducing agent and metal ions.
[0023] It is particularly preferred that the reducing electrolyzed
water of the present invention be produced by electrolyzing water
containing a reducing agent and metal ions to form electrolyzed
alkaline water having a pH of 9 to 12, an oxidation-reduction
potential of up to -600 mV and a dissolved oxygen content of up to
3 ppm, and adding a reducing agent to the electrolyzed alkaline
water.
[0024] Furthermore, it is preferred that the reducing electrolyzed
water of the present invention be produced by electrolyzing water
containing a reducing agent and metal ions to form electrolyzed
alkaline water having a pH of 9 to 12, an oxidation-reduction
potential of up to -600 mV and a dissolved oxygen content of up to
3 ppm, and then further electrolyzing the electrolyzed alkaline
water.
[0025] The reducing agent used in the method for producing the
reducing electrolyzed water of the present invention includes a
mixture having a g-lactone structure (a cyclic ester obtained by
ring closure through cyclodehydration of a carboxylic acid and a
hydroxyl group within the molecule) and an OH group, or a
saccharide having a five- or six-membered ring containing oxygen
and at least one OH group. Examples of the reducing agent include
saccharides such as vitamin C, glucose, fructose and lactose, and
erythorbic acid (isoascorbic acid).
[0026] Furthermore, examples of the reducing agent of the present
invention other than the reducing agents mentioned above include
oxalacetic acid, vitamin E, EDTA (ethylene-diaminetetraacetic acid)
and isopropyl citrate.
[0027] Examples of the metal ions used in the method for producing
the reducing electrolyzed water of the present invention are all
the metal ions. of the metal ions, particularly preferred examples
are sodium ions, potassium ions, calcium ions and magnesium
ions.
[0028] Preferred examples of the source water to be electrolyzed
include ultrapure water, pure water, purified water and distilled
water. However, various types of service water may also be
used.
[0029] When the reducing electrolyzed water of the present
invention thus produced is allowed to stand for a long period of
time, the oxidation-reduction potential may sometimes increase from
-200 to about 0 mV. However, the reducing electrolyzed water having
an oxidation-reduction potential of up to -200 mV is restored by
adding an aqueous solution of a hydroxide such as lithium
hydroxide, sodium hydroxide, potassium hydroxide, rubidium
hydroxide, cesium hydroxide and calcium hydroxide.
BRIEF DESCRIPTION OF THE DRAWINGS
[0030] FIG. 1 is a cross-sectional view showing an apparatus for
producing electrolytic water used in examples of the present
invention.
[0031] FIG. 2 is a cross-sectional view showing an apparatus for
producing electrolytic water used in examples of the present
invention.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0032] An example of the present invention will be explained by
making reference to drawings.
EXAMPLE 1
[0033] As shown in FIG. 1, in the present example, a diaphragm 4
was arranged in the central portion of an electrolyzer 1 (100 mm
wide, 200 mm long and 100 mm high) having a capacity of 2 liters so
that the electrolyzer was compartmented to form electrolyzer
chambers 5, 6 each having a capacity of 1 liter. Moreover,
electrode plates 2, 3 were arranged in the bath in such a manner
that one of the principal surface of the electrode plate 2 and one
of the principal surface of the electrode plate 3 faced each other,
and that a distance L between the electrode plates 2, 3 became 4
mm. Two platinum-plated titanium plates each having a length of 114
mm and a width of 74 mm were used as the electrode plates 2, 3
provided in the electrolyzer 1.
[0034] In the present example, water prepared by adding 0.25 g of
vitamin C and from 0.01 to 0.02 g of calcium chloride to 2 liters
of service water was filled in the electrolyzer 1. The water was
electrolyzed by applying a constant voltage of 25 V (maximum
current: 2.2 A) between both electrode plates 2, 3 for 20 minutes.
Measurements were made on the electrolyzed alkaline water thus
produced, and the water was found to have a pH of 10.69, an
oxidation-reduction potential (ORP) of -813 mV and a dissolved
oxygen content (DO) of 1.47 ppm.
[0035] Next, 0.25 g of vitamin C was added to 2 liters of the
electrolytic water to give reducing electrolyzed water. The same
measurements were made on the water, and the results are shown in
Table 1.
[0036] In addition, the pH was measured using a pH meter D-13 and a
pH sensor #6350-10D (trade names, manufactured by Horiba Ltd.). The
ORP was measured using an ORP meter D13 and an ORP sensor #6860-10C
(trade names, manufactured by Horiba Ltd.). The DO was measured
using a DO meter DO14-P and a DO sensor OE-2102 (trade names,
manufactured by Toa Electronics Ltd.).
[0037] According to the present example, the ORP and the DO can be
made small while a desired pH is being obtained. Moreover, when
water is to be produced in accordance with the application, a
desired reducing electrolyzed water can be simply obtained, for
example, by producing only one type of water having a pH of 9 to 12
and subsequently adding a reducing agent such as vitamin C, without
using a specific apparatus after preparation of the one type of
water. Furthermore, when a reducing agent is added in a certain
ratio before and after electrolysis, the balance of the reducing
agent subsequent to the production of the water is improved, and
the reducing capability is increased.
EXAMPLE 2
[0038] Using the same apparatus for producing electrolytic water as
in Example 1, water prepared by adding from 0.01 to 0.02 g of
calcium chloride to 2 liters of service water was filled in the
electrolyzer 1. The water was electrolyzed by applying a constant
voltage of 25 V (maximum current: 2.2 A) between both electrode
plates 2, 3 for 20 minutes. Measurements were made on the
electrolyzed alkaline water thus produced, and the water was found
to have a pH of 10.29, an ORP of -790 mV and a DO of 1.00 ppm.
[0039] Next, 0.5 g of vitamin C was added to 2 liters of the
electrolytic water to give reducing electrolyzed water. The same
measurements were made on the water, and the results are shown in
Table 1.
[0040] According to the present example, the ORP and the DO can be
made small while a desired pH is being obtained. Moreover, when a
highly reactive reducing agent is used, aging of the reducing
capability becomes a problem. In the present example, desired
reducing electrolyzed water can be obtained by electrolyzing water
containing such metal ions as formed from calcium chloride and
adding the reducing agent at the time of using the reducing
electrolyzed water. Accordingly, the reducing electrolyzed water is
excellent in preserving the reducing capability.
EXAMPLE 3
[0041] Using the same apparatus for producing electrolytic water as
in Example 1, water prepared by adding from 0.01 to 0.02 g of
calcium chloride to 2 liters of service water was filled in the
electrolyzer 1. The water was electrolyzed by applying a constant
voltage of 25 V (maximum current: 2.2 A) between both electrode
plates 2, 3 for 20 minutes. Measurements were made on the
electrolyzed alkaline water thus produced, and the water had a pH
of 11.02, an ORP of -840 mV and a DO of 3.00 ppm.
[0042] Next, 0.5 g of vitamin C was added to 2 liters of the
electrolytic water to give reducing electrolyzed water. The same
measurements were made on the water, and the water was found to
have a pH of 10.05, an ORP of -770 mV and a DO of 3.00 ppm.
[0043] Furthermore, 0.5 g of vitamin C was added to 2 liters of the
electrolyzed alkaline water to give reducing electrolyzed water.
The same measurements were made on the water, and the results are
shown in Table 1.
[0044] According to the present example, the ORP and the DO can be
made small while a desired pH is being obtained, in the same manner
as in Example 2. Moreover, when a highly reactive reducing agent is
used, aging of the reducing capability becomes a problem. In the
present example, desired reducing electrolyzed water can be
obtained by electrolyzing water containing such metal ions as
formed from calcium chloride and adding the reducing agent at the
time of using the reducing electrolyzed water. Accordingly, the
reducing electrolyzed water is excellent in preserving the reducing
capability.
EXAMPLE 4
[0045] Using the same apparatus for producing electrolytic water as
in Example 1, water prepared by adding 1 g of vitamin C and from
0.01 to 0.02 g of calcium chloride to 2 liters of service water was
filled in the electrolyzer 1. The water was electrolyzed by
applying a constant voltage of 25 V (maximum current: 2.2 A)
between both electrode plates 2, 3 for 20 minutes. Measurements of
the pH, the ORP and the DO were made on the reducing electrolyzed
water thus produced, and the results are shown in Table 1.
[0046] According to the present example, reducing electrolyzed
water can-be produced most simply.
EXAMPLE 5
[0047] Using the same apparatus for producing electrolytic water as
in Example 1, water prepared by adding 1 g of vitamin C and 0.1 g
of calcium chloride to 2 liters of service water was filled in the
electrolyzer 1. The water was electrolyzed by applying a constant
voltage of 12 V (maximum current: 0.5 A) between both electrode
plates 2, 3 for 3 hours. Measurements of the pH, the ORP and the DO
were made on the reducing electrolyzed water thus produced, and the
results are shown in Table 1.
[0048] According to the present example, reducing electrolyzed
water can be produced most simply.
EXAMPLE 6
[0049] First, using the apparatus for producing electrolytic water
shown in FIG. 1, water prepared by adding 0.5 g of vitamin C and
0.1 g of sodium chloride to 2 liters of supply water was filled in
the electrolyzer 1, and electrolyzed for 40 minutes. Measurements
were made on the electrolyzed alkaline water thus produced to find
that the water had a pH of 10.03, an ORP of -850 mV and a DO of
0.42 ppm.
[0050] Next, using an apparatus for producing electrolytic water
shown in FIG. 2, the electrolyzed alkaline water was electrolyzed
for 10 minutes by applying a constant voltage of 6 V between two
electrodes 2, 3. As shown in FIG. 2, in the apparatus for producing
electrolytic water, a pair of electrode plates 2, 3 were arranged
in a bath 1 (100 mm wide, 200 mm long and 100 mm high) having a
capacity of 2 liters in such a manner that one of the principal
surface of the electrode plate 2 and one of the principal surface
of the electrode plate 3 faced each other, and a bag-like diaphragm
4 was provided so that the diaphragm surrounded only one of the two
electrodes (an anode plate). A distance L between the electrode
plates 2, 3 was 4 mm. Two platinum-plated titanium plate each
having a length of 50 mm and a width of 6 mm were used as the
electrode plates 2, 3 provided in the electrolyzer 1. Measurements
of the pH, the ORP and the DO were made on the reducing
electrolyzed water thus formed, and the results are shown in Table
1.
[0051] According to the present example, the pH can be lowered to
about 7 while the ORP is being kept low. Accordingly, the reducing
electrolyzed water may be used for injections, etc.
Comparative Example 1
[0052] As a comparative example of Example 6, electrolyzed alkaline
water produced with the apparatus for producing electrolytic water
shown in FIG. 1 and having a pH of 10.03, an ORP of -850 mV and a
DO of 0.42 ppm was electrolyzed using the same apparatus.
Measurements of the pH, the ORP and the DO were made on the
electrolytic water on the acidic side thus produced, and the
results are shown in Table 1.
1 TABLE 1 PH ORP (mV) DO (ppm) Example 1 10.02 -773 1.11 Example 2
9.90 -766 1.00 Example 3 6.85 -570 1.15 Example 4 7.17 -560 1.54
Example 5 5.60 -540 0.66 Example 6 8.89 -724 0.43 Comparative
Example 1 6.31 +45 7.56
EXAMPLE 7
[0053] Next, using the reducing electrolyzed water of the present
invention, the extinction activity for active oxygen within an
animal body was evaluated. Specifically, a healthy dog was put on a
drip of reducing electrolyzed water having a pH of 6.50, an ORP of
-550 mV and a DO of 1.0 ppm for 24 hours. Blood plasma samples were
obtained before and after drip. The extinction activity for
O.sub.2.sup.- in the samples was measured with an ESR. The blood
plasma sample prior to drip showed an extinction activity for
O.sub.2.sup.- of 7.8 units/ml, an active oxygen content of 89.20
units/ml and an average value of superoxide dismutase (SOD) of
7.953. On the other hand, the blood plasma sample subsequent to
drip showed an increased value of an extinction activity for
O.sub.2.sup.- of 15.2 units/ml, a decreased value of an active
oxygen content of 58.00 units/ml and an increased value of an
average value of SOD of 14.627. That is, it has been confirmed that
use of the reducing electrolyzed water of the present invention as
a drip solution can decrease the active oxygen content within an
animal body.
EXAMPLE 8
[0054] Next, animal test was conducted to confirm the effects of
the reducing electrolyzed water on specific disease.
[0055] As a first case, a dog infected with lymphoma was put on a
drip of 200 ml of the same reducing electrolyzed water as in
Example 7. In order to confirm the state of the dog, measurements
of the amount of white blood cells (WBCs) and that of platelets
(PLTs) which are typical characteristics of the lymphoma were made.
The dog infected with lymphoma had an amount of WBCs of 55,800/ml
and an amount of PLTs of 39,000/ml before conducting drip. On the
other hand, the amount of WBCs decreased to 34,500/ml, and the
amount of PLTs increased to 106,000/ml 12 hours after drip. The dog
restored its appetite, and showed no difference in appearance
compared with other healthy dogs. It has thus been confirmed that
use of the reducing electrolyzed water as a drip solution exerts
significant effects on the disease of lymphoma.
EXAMPLE 9
[0056] As a second case, a dog infected with brachychronic
hepatargia was put on a drip of 720 ml of the same reducing
electrolyzed water as in Example 7, and the amounts of albumin
(ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN),
cholesterol (CHOL), creatinine (CREA), phosphoric acid (PHOS),
total bilirubine (T-Bil), total protein (TP) and globulin (GLOB)
which are the typical characteristics of brachychronic hepatargia
were measured. The results are shown in Table 2. It has thus been
confirmed that use of the reducing electrolyzed water of the
present invention as a drip solution exerts significant effects on
the disease of brachychronic hepatargia with regard to all the
characteristics mentioned above.
2 TABLE 2 Prior 22 hours 48 hours 96 hours to drip after drip after
drip after drip ALB (g/dl) 3.89 g/dl 3.01 2.89 2.83 ALP (IV) 562 IV
348 295 228 BUN (mg/dl) 106.5 mg/dl 26.3 4.6 1.8 CHOL (mg/dl) 387.6
mg/dl 253.6 239.1 212.4 CREA (mg/dl) 1.63 mg/dl 0.54 0.6 0.46 PHOS
(mg/dl) 11.74 mg/dl 3.38 -- 1.99 T-Bil (mg/dl) 1.22 mg/dl 0.41 --
-- TP (mg/dl) 8.76 mg/dl 6.62 6.56 6.42 GLOB (mg/dl) 4.87 mg/dl
3.62 3.67 3.59
EXAMPLE 10
[0057] As a third case, a dog infected with steroidal hepatitis was
put on a drip of 720 ml of the same reducing electrolyzed water as
in Example 7 for 72 hours, and the amount of alkaline phosphatase
(ALP) which is a typical characteristic of the disease was
measured. The amount of ALP was 2,171 units/ml before drip, and the
amount decreased to the following values: 1,293 units/ml 8 hours
after drip, 912 units/ml 16 hours after drip, 739 units/ml 24 hours
after drip, and 621 units/ml 32 hours after drip. It has thus been
confirmed that use of the reducing electrolyzed water of the
present invention as a drip solution exerts significant effects on
the disease of steroidal hepatitis.
[0058] It has been confirmed that the reducing electrolyzed water
of the present invention has significant medical effects when used
as a drink, in addition to use as drip solutions in Examples 7 to
10 mentioned above. As an example, 500 mice were made to drink the
reducing electrolyzed water of the present invention for 500 days.
The drink exerted no harmful influence on the mice, and the
survival rate increased to about 8 times as much with significance.
Moreover, the number of T cells increased, and the level of
aliphatic hydrogen peroxides in the blood serum lowered.
Furthermore, SOD increased.
EXAMPLE 11
[0059] It has been confirmed by the present inventors that the ORP
of the reducing electrolyzed water of the present invention
increases when allowed to stand still for a long period of time.
However, it has also been confirmed that the ORP is recovered to
the initial ORP when an aqueous solution containing hydroxide ions
are dropwise added. Experiments related to the potential properties
of the reducing electrolyzed water of the present invention were
conducted.
[0060] That is, reducing electrolyzed water having a pH of 5.68 and
an ORP of -530 mV was produced by the method of the present
invention described above. The water was placed in a container
having an open upper end, and allowed to stand still in a room for
20 hours. The pH and the ORP changed to 6.40 and 0 mV,
respectively.
[0061] A 1 N NaOH solution was added to 200 ml of the reducing
electrolyzed water in an amount of 1 to 200 ml, and the changes of
the pH and the ORP were measured. The same experiment was repeated
twice. The results are shown in Table 3.
3 TABLE 3 Once Twice 1N--NaOH pH ORP pH ORP 1 ml 11.24 -320 mV
11.07 -240 mV 2 ml 11.73 -625 mV 11.49 -659 mV 3 ml 12.08 -659 mV
11.73 -745 mV 4 ml 12.12 -655 mV 11.89 -771 mV 5 ml 12.27 -628 mV
11.99 -614 mV 6 ml 12.31 -599 mV 12.07 -570 mV 7 ml 12.37 -574 mV
12.12 -535 mV 8 ml 12.41 -534 mV 12.18 -515 mV 9 ml 12.47 -518 mV
12.24 -444 mV 10 ml 12.50 -495 mV 12.28 -384 mV 20 ml 12.72 -485 mV
12.49 -485 mV 30 ml 12.83 -465 mV 12.64 -465 mV 40 ml 12.92 -431 mV
12.72 -431 mV 50 ml 13.00 -415 mV 12.77 -415 mV 60 ml 13.05 -380 mV
12.82 -380 mV 70 ml 13.08 -350 mV 12.87 -350 mV 80 ml 13.10 -342 mV
12.91 -342 mV 90 ml 13.13 -320 mV 12.94 -320 mV 100 ml 13.06 -310
mV 13.96 -310 mV 200 ml 13.24 -290 mV 13.03 -290 mV
EXAMPLE 12
[0062] In the same manner as in Example 11, a 1 N KOH solution in
an amount of 1 to 200 ml was added to 200 ml of the reducing
electrolyzed water which had changed its pH and ORP to 6.40 and 0
mV, respectively when allowed to stand still, and the changes of
the pH and ORP were measured. The same experiments were repeated
twice. The results are shown in Table 4.
4 TABLE 4 Once Twice 1N--KOH pH ORP pH ORP 1 ml 11.00 -260 mV 10.98
-225 mV 2 ml 11.51 -666 mV 11.46 -630 mV 3 ml 11.73 -750 mV 11.71
-670 mV 4 ml 11.88 -755 mV 11.85 -650 mV 5 ml 11.91 -665 mV 11.95
-636 mV 6 ml 12.00 -600 mV 12.02 -620 mV 7 ml 12.09 -570 mV 12.08
-565 mV 8 ml 12.16 -530 mV 12.15 -550 mV 9 ml 12.22 -505 mV 12.21
-490 mV 10 ml 12.28 -488 mV 12.25 -480 mV 20 ml 12.44 -485 mV 12.48
-485 mV 30 ml 12.68 -460 mV 12.63 -450 mV 40 ml 12.78 -445 mV 12.72
-425 mV 50 ml 12.87 -415 mV 12.79 -420 mV 60 ml 12.93 -380 mV 12.84
-400 mV 70 ml 13.00 -350 mV 12.90 -380 mV 80 ml 13.03 -342 mV 12.94
-370 mV 90 ml 13.06 -320 mV 13.00 -365 mV 100 ml 13.08 -310 mV
13.07 -360 mV 200 ml 13.17 -290 mV 13.13 -330 mV
EXAMPLE 13
[0063] In the same manner as in Example 11, a 1 N Ca(OH).sub.2
solution in an amount of 1 to 7 ml was added to 200 ml of the
reducing electrolyzed water which had changed its pH and ORP to
6.40 and 0 mV, respectively when allowed to stand still. The
changes of the pH and the ORP were measured. The results are shown
in Table 5.
5TABLE 5 1N--Ca(OH).sub.2 pH ORP 1 ml 11.25 -225 mV 2 ml 11.72 -630
mV 3 ml 11.89 -670 mV 4 ml 12.00 -650 mV 5 ml 12.06 -636 mV 6 ml
12.10 -620 mV 7 ml 12.12 -565 mV
Comparative Example 2
[0064] As a comparative Examples 11 to 13, electrolytic water
having a pH of 7.43 and an ORP of -180 mV was allowed to stand
still in a room for 20 hours. The pH showed no change
substantially, and the ORP changed to +250 mV.
[0065] A 1 N NaOH solution in an amount of 1 to 200 ml was
subsequently added to 200 ml of the electrolytic water, and the
changes of the pH and the ORP were measured. The results are shown
in Table 6.
6TABLE 6 1N--NaOH pH ORP (mV) 1 ml 11.3 +70 5 ml 12.31 +60 10 ml
12.65 +39 50 ml 13.13 +12 100 ml 13.3 +20
[0066] It has been clearly confirmed from the results of Tables 3
to 6 that although the characteristic values of electrolytic water
such as the pH and the ORP change when it is allowed to stand still
for a long period of time, the reducing electrolyzed water of the
present invention potentially has the extinction activity for
active oxygen, and that even when the apparent characteristic
values change, addition of hydroxide ions, etc. can restore the
values.
* * * * *