U.S. patent application number 10/235026 was filed with the patent office on 2003-05-01 for dna encoding a novel human inhibitor-of-apoptosis protein.
This patent application is currently assigned to Schering Aktiengesellschaft. Invention is credited to Deng, Gang, Lin, Jiing-Huey, Morser, Michael John.
Application Number | 20030082725 10/235026 |
Document ID | / |
Family ID | 22432678 |
Filed Date | 2003-05-01 |
United States Patent
Application |
20030082725 |
Kind Code |
A1 |
Deng, Gang ; et al. |
May 1, 2003 |
DNA encoding a novel human inhibitor-of-apoptosis protein
Abstract
The present invention relates to novel human
inhibitor-of-apoptosis polypeptides, designated HIAP3,
polynucleotides encoding the polypeptides, methods for producing
the polypeptides, expression vectors and genetically engineered
host cells for expression of the polypeptides. The invention
further relates to methods for utilizing the polynucleotides and
polypeptides in research, diagnosis, and therapeutic
applications.
Inventors: |
Deng, Gang; (Benicia,
CA) ; Lin, Jiing-Huey; (Alamo, CA) ; Morser,
Michael John; (San Francisco, CA) |
Correspondence
Address: |
BERLEX BIOSCIENCES
LEGAL DEPARTMENT
15049 SAN PABLO AVENUE
P.O. BOX 4099
RICHMOND
CA
94804-0099
US
|
Assignee: |
Schering Aktiengesellschaft
Berlin
DE
D 13342
|
Family ID: |
22432678 |
Appl. No.: |
10/235026 |
Filed: |
September 3, 2002 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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10235026 |
Sep 3, 2002 |
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09127928 |
Jul 31, 1998 |
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6472172 |
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Current U.S.
Class: |
435/69.1 ;
435/184; 435/320.1; 435/325; 435/7.23; 536/23.2 |
Current CPC
Class: |
A61P 25/14 20180101;
C07K 14/4703 20130101; A61P 25/28 20180101; A61P 35/00 20180101;
A61P 37/02 20180101; A61P 9/10 20180101; A61K 48/00 20130101; A61P
31/12 20180101; A61K 38/00 20130101; A61P 9/04 20180101 |
Class at
Publication: |
435/69.1 ;
435/184; 435/320.1; 435/325; 536/23.2; 435/7.23 |
International
Class: |
G01N 033/574; C07H
021/04; C12N 009/99; C12P 021/02; C12N 005/06 |
Claims
What is claimed is:
1. An isolated polynucleotide comprising a polynucleotide having at
least a 70% identity to a member selected from the group consisting
of: (a) a polynucleotide encoding a polypeptide comprising the
amino acid sequence set forth in FIG. 2; (b) a polynucleotide
encoding a polypeptide comprising amino acid 88 to amino acid 289
as set forth in FIG. 2; (c) a polynucleotide encoding a polypeptide
comprising amino acid 88 to amino acid 155 as set forth in FIG. 2;
(d) a polynucleotide encoding a polypeptide comprising amino acid
240 to amino acid 289 as set forth in FIG. 2; and (e)
polynucleotide which is complementary to the polynucleotide of (a),
(b), (c) or (d).
2. The polynucleotide of claim 1 wherein the polynucleotide is
DNA.
3. The polynucleotide of claim 1 wherein the polynucleotide is
RNA.
4. The polynucleotide of claim 1 wherein the polynucleotide is
genomic DNA.
5. The polynucleotide of claim 2 wherein the polynucleotide encodes
the polypeptide comprising amino acids 1 to 298 as set forth in
FIG. 2.
6. The polynucleotide of claim 2 wherein the polynucleotide encodes
the polypeptide comprising amino acid 88 to amino acid 289 as set
forth in FIG. 2.
7. The polynucleotide of claim 2 wherein the polynucleotide encodes
the polypeptide comprising amino acid 88 to amino acid 155 as set
forth in FIG. 2.
8. The polynucleotide of claim 2 wherein the polynucleotide encodes
the polypeptide comprising amino acid 240 to amino acid 289 as set
forth in FIG. 2.
9. The polynucleotide of claim 1 wherein the polynucleotide
comprises the sequence as set forth in FIG. 1 from nucleotide 1 to
nucleotide 1337.
10. The polynucleotide of claim 1 wherein the polynucleotide
comprises the sequence as set forth in FIG. 1 from nucleotide 170
to nucleotide 1064.
11. An vector comprising the polynucleotide of claim 2.
12. A host cell comprising the vector of claim 11.
13. A method of producing a polypeptide comprising expressing from
the host cell of claim 12 the polypeptide encoded by the
polynucleotide.
14. The method of claim 13 wherein the polypeptide comprises amino
acid 1 to amino acid 298 as set forth in FIG. 2.
15. The method of claim 13 wherein the polypeptide comprises amino
acid 88 to amino acid 289 as set forth in FIG. 2.
16. The method of claim 13 wherein the polypeptide comprises amino
acid 88 to amino acid 155 as set forth in FIG. 2.
17. The method of claim 13 wherein the polypeptide comprises amino
acid 240 to amino acid 289 as set forth in FIG. 2.
18. A method of producing a polypeptide wherein the polypeptide
comprises the amino acid sequence shown in FIG. 2, the method
comprising the steps of: (a) culturing the host cell of claim 12
under conditions whereby the polypeptide is expressed; and (b)
recovering the polypeptide from the culture.
19. A method for producing a cell which expresses a polypeptide
comprising genetically engineering the cell with the vector of
claim 11.
20. A polypeptide comprising a member selected from the group
consisting of: (a) a polypeptide having the amino acid sequence as
set forth in FIG. 2; (b) a polypeptide comprising amino acid 88 to
amino acid 289 as set forth in FIG. 2; (c) a polypeptide comprising
amino acid 88 to amino acid 155 as set forth in FIG. 2; (d) a
polypeptide comprising amino acid 240 to amino acid 289 as set
forth in FIG. 2; and (e) a polypeptide which is at least 70%
identical to the polypeptide of (a), (b), (c) or (d).
21. The polypeptide of claim 20 wherein the polypeptide comprises
amino acid 1 to amino acid 298 as set forth in FIG. 2.
21. The polypeptide of claim 20 wherein the polypeptide comprises
amino acid 88 to amino acid 289 as set forth in FIG. 2.
22. The polypeptide of claim 20 wherein the polypeptide comprises
amino acid 88 to amino acid 155 as set forth in FIG. 2.
23. The polypeptide of claim 20 wherein the polypeptide comprises
amino acid 240 to amino acid 289 as set forth in FIG. 2
24. A method of treating a disease-state in a human patient which
disease-state is associated with inappropriate apoptosis and
wherein the patient is in need of increased levels of a polypeptide
comprising a member selected from the group consisting of: (a) a
polypeptide having the amino acid sequence as set forth in FIG. 2;
(b) a polypeptide comprising amino acid 88 to amino acid 289 as set
forth in FIG. 2; (c) a polypeptide comprising amino acid 88 to
amino acid 155 as set forth in FIG. 2; (d) a polypeptide comprising
amino acid 240 to amino acid 289 as set forth in FIG. 2; and (e) a
polypeptide which is at least 70% identical to the polypeptide of
(a), (b), (c) or (d); wherein the method comprises administering to
the patient a therapeutically effective amount of the
polypeptide.
25. The method of claim 24 wherein said therapeutically effective
amount of the polypeptide is administered by providing to the
patient a polynucleotide encoding the polypeptide and expressing
the polypeptide in vivo.
26. A method of treating a disease-state in a human patient which
disease-state is associated with inappropriate apoptosis and
wherein the patient is in need of decreased levels of a polypeptide
comprising a member selected from the group consisting of: (a) a
polypeptide having the amino acid sequence as set forth in FIG. 2;
(b) a polypeptide comprising amino acid 88 to amino acid 289 as set
forth in FIG. 2; (c) a polypeptide comprising amino acid 88 to
amino acid 155 as set forth in FIG. 2; (d) a polypeptide comprising
amino acid 240 to amino acid 289 as set forth in FIG. 2; and (e) a
polypeptide which is at least 70% identical to the polypeptide of
(a), (b), (c) or (d); wherein the method comprises administering to
the patient a therapeutically effective amount of a ribozyme which
specifically cleaves RNA encoding the polypeptide.
27. A method of treating a disease-state in a human patient which
disease-state is associated with inappropriate apoptosis and
wherein the patient is in need of decreased levels of a polypeptide
comprising a member selected from the group consisting of: (a) a
polypeptide having the amino acid sequence as set forth in FIG. 2;
(b) a polypeptide comprising amino acid 88 to amino acid 289 as set
forth in FIG. 2; (c) a polypeptide comprising amino acid 88 to
amino acid 155 as set forth in FIG. 2; (d) a polypeptide comprising
amino acid 240 to amino acid 289 as set forth in FIG. 2; and (e) a
polypeptide which is at least 70% identical to the polypeptide of
(a), (b), (c) or (d); awherein the method comprises administering
to the patient a therapeutically effective amount of a
polynucleotide which is complementary to a polynucleotide encoding
the polypeptide.
28. A diagnostic method wherein the method comprises analyzing for
the presence of a polypeptide comprising a member selected from the
group consisting of: (a) a polypeptide having the amino acid
sequence as set forth in FIG. 2; (b) a polypeptide comprising amino
acid 88 to amino acid 289 as set forth in FIG. 2; (c) a polypeptide
comprising amino acid 88 to amino acid 155 as set forth in FIG. 2;
(d) a polypeptide comprising amino acid 240 to amino acid 289 as
set forth in FIG. 2; and (e) a polypeptide which is at least 70%
identical to the polypeptide of (a), (b), (c) or (d); in a sample
derived from a host.
29. A diagnostic method wherein the method comprises analyzing for
the presence of a polynucleotide comprising a polynucleotide having
at least 70% identity to a member selected from the group
consisting of: (a) a polynucleotide encoding a polypeptide
comprising the amino acid sequence set forth in FIG. 2; (b) a
polynucleotide encoding a polypeptide comprising amino acid 88 to
amino acid 289 as set forth in FIG. 2; (c) a polynucleotide
encoding a polypeptide comprising amino acid 88 to amino acid 155
as set forth in FIG. 2; (d) a polynucleotide encoding a polypeptide
comprising amino acid 240 to amino acid 289 as set forth in FIG. 2;
and (e) polynucleotide which is complementary to the polynucleotide
of (a), (b), (c) or (d); in a sample derived from a host.
30. An isolated antibody which is specific for a polypeptide
comprising a member selected from the group consisting of: (a) a
polypeptide having the amino acid sequence as set forth in FIG. 2;
(b) a polypeptide comprising amino acid 88 to amino acid 289 as set
forth in FIG. 2; (c) a polypeptide comprising amino acid 88 to
amino acid 155 as set forth in FIG. 2; (d) a polypeptide comprising
amino acid 240 to amino acid 289 as set forth in FIG. 2; and (e) a
polypeptide which is at least 70% identical to the polypeptide of
(a), (b), (c) or (d).
Description
FIELD OF THE INVENTION
[0001] This invention relates, in part, to newly identified
polynucleotides and polypeptides; variants and derivatives of the
polynucleotides and polypeptides; methods of making the
polynucleotides and polypeptides, and their variants and
derivatives; and uses of the polynucleotides, polypeptides,
variants, and derivatives. In particular, in these and in other
regards, the invention relates to novel human
inhibitor-of-apoptosis polypeptides and the polynucleotides which
encode these polypeptides.
BACKGROUND OF THE INVENTION
[0002] Apoptosis, also known as programmed cell death, is a
genetically controlled process which plays an important role in
development and in cellular and tissue homeostasis (Hengartner, M.,
Exp. Gerontol. 32:363-374, 1997; Hoeppner et al., Biochem. Biophys.
Acta 1242:217-220, 1996; Ellis et al., Annual Rev. Cell Biol.
7:663-698, 1991). Apoptosis permits the elimination of cells which
either have been overproduced, developed improperly or have
undergone genetic damage and represents a major host defense
mechanism for limiting the replication of infective viruses. In
contrast to necrotic cell death, which is usually accompanied by
swelling and disruption of cellular membranes and inflammation of
adjacent tissue, apoptosis is marked by cell shrinkage, blebbing,
chromatin condensation, DNA fragmentation and formation of
apoptotic bodies (MacLellan and Schneider, Circ. Res. 81:137-144,
1997; Cohen, G., Biochem. J. 326 (Pt 1):1-16, 1997). Apoptotic
cells are then phagocytosed by neighboring scavenger cells without
eliciting an inflammatory response (Wu and Horvitz, Nature
392:501-504, 1998).
[0003] Deregulation of apoptosis has been implicated in the
pathogenesis of a variety of diseases. Impaired apoptosis can play
a role in cancer (Pan et al., Cancer Surv. 29:305-327, 1997;
Thompson, C., Science 267:1456-1462, 1995) or chronic viral
infection (Clem et al., Science 254:1388-1390, 1991; Clem and
Miller, Mol. Cell. Biol. 14:5212-5222, 1994). Inappropriate (or
premature) apoptosis may contribute to neurodegenerative disorders
(Roy et al., Cell 80:167-178, 1995; Raff et al., Science
262:695-700, 1996) or acquired immunodeficiency disease (Banda, N.,
J. Exp. Med. 176:1099-1106, 1992). Premature apoptosis is also
recognized as a contributing cause of myocyte loss in
ischemia/repurfusion injury, myocardial infarction (MacLellan and
Schneider, Circ. Res. 81:137-144, 1997), and congestive heart
failure (Feuerstein, G., Trends Cardiovas. Med. 7:249-255,
1997).
[0004] The presence of a novel class of apoptosis inhibitors, known
as inhibitor of apoptosis proteins (IAPs) has been reported in the
literature (Liston et al., Apoptosis 2:423-441, 1997). The first
IAP was discovered in baculovirus (Crook et al., J. Vir.
67:2166-2174, 1993) and IAPs have now been reported in Drosophila,
chick, mouse and human (Hay et al., Cell 83:1253-1262, 1995; Liston
et al., supra). Five human IAPs have been identified: HIAP1, HIAP2,
XIAP (X-chromosome linked IAP), NIAP (neuronal IAP) and survivin
(Ambrosini et al., Nat Med. 3:917-921, 1997; Duckett et al., Embo
J. 15:2685-2694, 1996).
[0005] IAPs are a highly evolutionarily conserved family of
proteins, containing a number of common structural features
(domains). Among these are an N-terminal domain containing one or
more repeats of a domain referred to as the BIR (baculovirus IAP
repeat) domain (Liston et al., supra), and a C-terminal RING zinc
finger domain. These domains are present to varying degrees within
the known members of the IAP family; HIAP1 and HIAP2 contain three
BIR domains and a C-terminal RING domain, while survivin contains
only a single BIR domain and no RING domain.
[0006] While the physiological role of IAPs is not exactly clear,
some members of the IAP family appear to play a regulatory role in
apoptosis. Recombinant IAPs were found to suppress apoptosis
induced by a variety of stimuli in different cell types. Drosophila
IAPs (DIAP1 and DIAP2) were found to interact with a
Decapentaplegic (Dpp) type I receptor, suggesting that these DIAPs
may act as negative regulators of the Dpp signaling pathway, which
normally leads to cell apoptosis. XIAP, HIAP1 and HIAP2 can
directly inhibit specific caspases (cysteine containing aspartate
specific proteases), enzymes which are involved in the pathways
which control apoptosis, and thereby suppress apoptosis
(Thornberry, N., Br. Med. Bull. 53:478-490, 1997). However, NIAP
was found not to inhibit caspases, suggesting that different IAPs
may have different mechanisms of action.
[0007] By helping in the regulation of programmed cell death, IAPs
play an important role in the maintenance of the appropriate life
cycle of the various cells of an organism. It is likely that
variance from normal levels (either overabundance or deficiency) of
IAPs within the cellular environment may lead to conditions in vivo
which are related to various disease states.
[0008] IAPs may play a role in tumor formation. Up-regulated
chicken IAP and concommitant suppression of apoptosis were found in
chicken cells transformed by the oncoprotein v-rel, a member of the
Rel/NF.sub.kappaB family (You et al., Mol. Cell. Biol.
17:7328-7341, 1997). Similarly, baculovirus protein p35 (a
baculovirus IAP) is capable of promoting the transformation of
mouse embryo fibroblasts in the presence of the insulin-like growth
factor I receptor (Resnicoff et al., J. Biol. Chem.
273:10376-10380, 1998).
[0009] Survivin is undetectable in terminally differentiated adult
tissues but expressed in all common human cancers, further
suggesting that apoptosis inhibition may be a general feature of
neoplasia (Ambrosini et al., supra).
[0010] Deletion mutations in human NIAP have been linked to
inappropriate depletion of motor neurons associated with spinal
muscular atrophy, an autosomal neurodegenerative disorder (Xu et
al., J. Comp. Neurol. 382:247-259, 1997). In a rat ischemia model,
in vivo overexpression of NIAP reduced ischemic damage in the rat
hippocampus (Roy et al., supra), indicating that the presence of
increased levels of IAPs could prevent the unwanted cell death
characteristic of ischemia and that elevating the neuronal levels
of this IAP may be useful in treating stroke.
[0011] Finally, Stellar and his colleagues were able to block
retinal cell death and show significant retention of visual
function in Drosophila which exhibited retinitus pigmentosa, a
cause of blindness in humans, by eye-specific expression of the
antiapoptotic protein p35 (Davidson and Stellar, Nature
391:587-591, 1998).
[0012] These data suggest that antiapoptotic proteins, such as
IAPs, are good candidates for use in the therapeutic intervention
of diseases caused by altered apoptosis.
[0013] There is a need, therefore, for identification and
characterization of proteins that influence apoptosis. In
particular, there is a need to isolate and characterize additional
IAPs, akin to known IAPs, which may be employed, therefore, for
ameliorating or correcting dysfunctions or disease associated with
inappropriate apoptosis; in cancer and chronic viral infections,
where IAPs may be overproduced, as well as in neurodegenerative
disorders, chronic heart failure and dysfunctional immune response,
where a deficiency in IAPs may exist.
SUMMARY OF THE INVENTION
[0014] The present invention provides a polynucleotide sequence
which uniquely encodes a novel human inhibitor-of-apoptosis
protein. Designated HIAP3, the polypeptide is characterized by
structural features common to the inhibitor-of-apoptosis protein
family, such as BIR and RING domains. The polynucleotide sequence,
designated in lower case, hiap3, and described in FIG. 1, encodes
the amino acid sequence, which is designated HIAP3, and is shown in
FIG. 2.
[0015] Toward these ends, and others, it is an object of the
present invention to provide polypeptides, inter alia, that have
been identified as a novel HIAP3 by homology between the amino acid
sequence set out in FIG. 2 and known amino acid sequences of other
IAP proteins.
[0016] It is a further object of the invention, moreover, to
provide polynucleotides that encode HIAP3, particularly
polynucleotides that encode the polypeptide herein designated
HIAP3.
[0017] In accordance with this aspect of the invention there are
provided isolated polynucleotides encoding HIAP3, including mRNAs,
cDNAs, genomic DNAs and, in further embodiments of this aspect of
the invention, biologically, diagnostically, clinically or
therapeutically useful variants, analogs or derivatives thereof, or
fragments thereof, including fragments of the variants, analogs and
derivatives.
[0018] Among the particularly preferred embodiments of this aspect
of the invention are naturally occurring allelic variants of hiap3
polynucleotides.
[0019] It also is an object of the invention to provide HIAP3
polypeptides that may be employed to treat neoplasia,
neurodegenerative disorders, immune disorders, chronic viral
infections, or chronic heart failure.
[0020] In accordance with this aspect of the invention there are
provided novel polypeptides of human origin referred to herein as
HIAP3 as well as biologically, diagnostically or therapeutically
useful fragments, variants and derivatives thereof, variants and
derivatives of the fragments, and analogs of the foregoing.
[0021] Among the particularly preferred embodiments of this aspect
of the invention are variants of HIAP3 encoded by naturally
occurring allelic variants of the hiap3 polynucleotide.
[0022] It is another object of the invention to provide a method of
producing the aforementioned polypeptides, polypeptide fragments,
variants and derivatives, fragments of the variants and
derivatives, and analogs of the foregoing. In a preferred
embodiment of this aspect of the invention there are provided
methods of producing the aforementioned HIAP3 polypeptides
comprising culturing host cells having expressibly incorporated
therein an exogenously-derived HIAP3-encoding polynucleotide under
conditions for expression of human HIAP3 in the host and then
recovering the expressed polypeptide.
[0023] In accordance with another object of the invention there are
provided products, compositions, processes and methods that utilize
the aforementioned polypeptides and polynucleotides for research,
biological, clinical and therapeutic purposes, inter alia.
[0024] In accordance with certain preferred embodiments of this
aspect of the invention, there are provided products, compositions
and methods, inter alia, for, among other things: assessing HIAP3
expression in cells by determining HIAP3 polypeptides or
HIAP3-encoding mRNA; assaying genetic variation and aberrations,
such as defects, in hiap3 genes; and administering an HIAP3
polypeptide or polynucleotide encoding HIAP3 to an organism to
alter the level of HIAP3 activity.
[0025] In accordance with certain preferred embodiments of this and
other aspects of the invention there are provided probes that
hybridize to hiap3 sequences.
[0026] In certain additional preferred embodiments of this aspect
of the invention there are provided antibodies against HIAP3
polypeptides. In certain particularly preferred embodiments in this
regard, the antibodies are highly selective for HIAP3, and may be
employed diagnostically to detect increased HIAP3 expression, which
may be associated with conditions in which inhibition of apoptosis
is too strong, such as cancer.
[0027] In a further aspect of the invention there are provided
compositions comprising an hiap3 polynucleotide or an HIAP3
polypeptide for administration to cells in vitro, to cells ex vivo
and to cells in vivo, or to a multicellular organism. In certain
particularly preferred embodiments of this aspect of the invention,
the compositions comprise an hiap3 polynucleotide for expression of
an HIAP3 polypeptide in a host organism for treatment of disease.
Particularly preferred in this regard is expression in a human
patient for treatment of a disease state which is alleviated by
increasing the level of HIAP3 activity.
[0028] In a further aspect of the invention there are provided
ribozymes and polynucleotides complementary to hiap3
polynucleotides (i.e. antisense polynucleotides) for administration
to cells in vitro, to cells ex vivo and to cells in vivo, or to a
multicellular organism. Particularly preferred in this regard is
administration to a human patient for treatment of a disease state
which is alleviated by decreasing the level of HIAP3 activity.
[0029] Other objects, features, advantages and aspects of the
present invention will become apparent to those of skill from the
following description. It should be understood, however, that the
following description and the specific examples, while indicating
preferred embodiments of the invention, are given by way of
illustration only. Various changes and modifications within the
spirit and scope of the disclosed invention will become readily
apparent to those skilled in the art from reading the following
description and from reading the other parts of the present
disclosure.
BRIEF DESCRIPTION OF THE DRAWINGS
[0030] FIG. 1 displays the polynucleotide sequence of hiap3, which
encodes HIAP3.
[0031] FIG. 2 displays the deduced amino acid sequence of
HIAP3.
[0032] FIG. 3 displays a schematic drawing of the functional
domains within an HIAP3 polypeptide.
[0033] FIG. 4 demonstrates the amino acid alignment of the BIR
domain of HIAP3 with the BIR domain of other members of the IAP
protein family.
[0034] FIG. 5 demonstrates the amino acid alignment of the RING
domain of HIAP3 with the RING domain of other members of the IAP
protein family.
[0035] The following drawings depict certain embodiments of the
invention. They are illustrative only and do not limit the
invention otherwise disclosed herein.
DETAILED DESCRIPTION OF THE INVENTION
[0036] Definitions
[0037] As used in the specification, examples and appended claims,
unless specified to the contrary, the following terms have the
meaning indicated.
[0038] "HIAP3" refers to the polypeptide having the amino acid
sequence set out in FIG. 2; variants, analogs, derivatives and
fragments thereof, and fragments of the variants, analogs and
derivatives. The terms "fragment," "derivative" and "analog" when
referring to the polypeptide of FIG. 2 mean a polypeptide which
retains essentially the same biological activities as the
polypeptide of FIG. 2.
[0039] "hiap3" refers to the polynucleotide having the sequence set
out in FIG. 1 and polynucleotides encoding polypeptides having the
amino acid sequence of HIAP3 set out in FIG. 2; and to
polynucleotides encoding HIAP3 variants, analogs, derivatives and
fragments, and fragments of the variants, analogs and derivatives.
hiap3 also refers to such polynucleotides composed of RNA as well
as to polynucleotides which are the complement of polynucleotides
which encode the polypeptide sequence set out in FIG. 2.
[0040] "Polynucleotide(s)" generally refers to any
polyribonucleotide or polydeoxribonucleotide, which may be
unmodified RNA or DNA or modified RNA or DNA. Thus, for instance,
polynucleotides as used herein refers to, among others, single-and
double-stranded DNA, DNA that is a mixture of single- and
double-stranded regions, single- and double-stranded RNA, and RNA
that is mixture of single- and double-stranded regions, hybrid
molecules comprising DNA and RNA that may be single-stranded or,
more typically, double-stranded or a mixture of single- and
double-stranded regions. In addition, polynucleotide as used herein
refers to triple-stranded regions comprising RNA or DNA or both RNA
and DNA. The strands in such regions may be from the same molecule
or from different molecules. The regions may include all of one or
more of the molecules, but more typically involve only a region of
some of the molecules. One of the molecules of a triple-helical
region often is an oligonucleotide.
[0041] As used herein, the term polynucleotide includes DNAs or
RNAs as described above that contain one or more modified bases.
Thus, DNAs or RNAs with backbones modified for stability or for
other reasons are "polynucleotides" as that term is intended
herein. Moreover, DNAs or RNAs comprising unusual bases, such as
inosine, or modified bases, such as tritium-labelled bases, to name
just two examples, are polynucleotides as the term is used
herein.
[0042] It will be appreciated that a great variety of modifications
have been made to DNA and RNA that serve many useful purposes known
to those of skill in the art. The term polynucleotide as it is
employed herein embraces such chemically, enzymatically or
metabolically modified forms of polynucleotides, as well as the
chemical forms of DNA and RNA characteristic of viruses and cells,
including simple and complex cells, inter alia.
[0043] "Polypeptides", as used herein, includes all polypeptides as
described below. The basic structure of polypeptides is well known
and has been described in innumerable textbooks and other
publications in the art. In this context, the term is used herein
to refer to any peptide or protein comprising two or more amino
acids joined to each other in a linear chain by peptide bonds. As
used herein, the term refers to both short chains, which also
commonly are referred to in the art as peptides, oligopeptides and
oligomers, for example, and to longer chains, which generally are
referred to in the art as proteins, of which there are many
types.
[0044] It will be appreciated that polypeptides often contain amino
acids other than the 20 amino acids commonly referred to as the 20
naturally occurring amino acids, and that many amino acids,
including the terminal amino acids, may be modified in a given
polypeptide, either by natural processes such as glycosylation and
other post-translational modifications, or by chemical modification
techniques which are well known in the art. Even the common
modifications that occur naturally in polypeptides are too numerous
to list exhaustively here, but they are well described in basic
texts and in more detailed monographs, as well as in a voluminous
research literature, and they are well known to those of skill in
the art. Among the known modifications which may be present in
polypeptides of the present invention are, to name an illustrative
few, acetylation, acylation, ADP-ribosylation, amidation, covalent
attachment of flavin, covalent attachment of a heme moiety,
covalent attachment of a polynucleotide or polynucleotide
derivative, covalent attachment of a lipid or lipid derivative,
covalent attachment of phosphotidylinositol, cross-linking,
cyclization, disulfide bond formation, demethylation, formation of
covalent cross-links, formation of cystine, formation of
pyroglutamate, formylation, gamma-carboxylation, glycation,
glycosylation, GPI anchor formation, hydroxylation, iodination,
methylation, myristoylation, oxidation, proteolytic processing,
phosphorylation, prenylation, racemization, selenoylation,
sulfation, transfer-RNA mediated addition of amino acids to
proteins such as arginylation, and ubiquitination.
[0045] Such modifications are well known to those of skill and have
been described in great detail in the scientific literature.
Several particularly common modifications, glycosylation, lipid
attachment, sulfation, gamma-carboxylation of glutamic acid
residues, hydroxylation and ADP-ribosylation, for instance, are
described in most basic texts, such as, for instance, I. E.
Creighton, Proteins-Structure and Molecular Properties, 2nd Ed., W.
H. Freeman and Company, New York, 1993. Many detailed reviews are
available on this subject, such as, for example, those provided by
Wold, F., in Posttranslational Covalent Modification of Proteins,
B. C. Johnson, Ed., Academic Press, New York, pp 1-12, 1983;
Seifter et al., Meth. Enzymol. 182:626-646, 1990 and Rattan et al.,
Protein Synthesis: Posttranslational Modifications and Aging, Ann.
N.Y. Acad. Sci. 663: 48-62, 1992.
[0046] It will be appreciated, as is well known and as noted above,
that polypeptides are not always entirely linear. For instance
polypeptides may be branched as a result of ubiquitination, and
they may be circular, with or without branching, generally as a
result of Posttranslational events, including natural processing
events and events brought about by human manipulation which do not
occur naturally. Circular, branched and branched circular
polypeptides may be synthesized by non-translational natural
processes and by entirely synthetic methods, as well.
[0047] Modifications can occur anywhere in a polypeptide, including
the peptide backbone, the amino acid side-chains and the amino or
carboxyl termini. In fact, blockage of the amino or carboxyl group
in a polypeptide, or both, by a covalent modification, is common in
naturally occurring and synthetic polypeptides and such
modifications may be present in polypeptides of the present
invention, as well. For instance, the amino terminal residue of
polypeptides made in E. coli, prior to proteolytic processing,
almost invariably will be N-formylmethionine.
[0048] The modifications that occur in a polypeptide often will be
a function of how it is made. For polypeptides made by expressing a
cloned gene in a host, for instance, the nature and extent of the
modifications in large part will be determined by the host cell
posttranslational modification capacity and the modification
signals present in the polypeptide amino acid sequence. For
instance, as is well known, glycosylation often does not occur in
bacterial hosts such as E. coli. Accordingly, when glycosylation is
desired, a polypeptide should be expressed in a glycosylating host,
generally a eukaryotic cell. Insect cells often carry out the same
posttranslational glycosylations as mammalian cells and, for this
reason, insect cell expression systems have been developed to
efficiently express mammalian proteins having native patterns of
glycosylation, inter alia. Similar considerations apply to other
modifications.
[0049] It will be appreciated that the same type of modification
may be present to the same or varying degree at several sites in a
given polypeptide. Also, a given polypeptide may contain many types
of modifications.
[0050] In general, as used herein, the term polypeptide encompasses
all such modifications, particularly those that are present in
polypeptides synthesized by expressing a polynucleotide in a host
cell.
[0051] "Polynucleotide encoding a polypeptide" as used herein
encompasses polynucleotides which include a sequence encoding a
polypeptide of the present invention, particularly the HIAP3 having
the amino acid sequence set out in FIG. 2. The term encompasses
polynucleotides that include a single continuous region or
discontinuous regions encoding the polypeptide (for example,
interrupted by introns) together with additional regions.
[0052] "Biological activity" refers to the biologic and/or
immunologic activities of naturally occurring HIAP3.
[0053] "Oligonucleotide(s)" refers to relatively short
polynucleotides. Often the term refers to single-stranded
deoxyribonucleotides, but it can refer as well to single- or
double-stranded ribonucleotides, RNA:DNA hybrids and
double-stranded DNAs, among others. Oligonucleotides, such as
single-stranded DNA probe oligonucleotides, often are synthesized
by chemical methods, such as those implemented on automated
oligonucleotide synthesizers. However, oligonucleotides can be made
by a variety of other methods, including in vitro recombinant
DNA-mediated techniques and by expression of DNAs in cells and
organisms. "Oligonucleotides" or "oligomers" or polynucleotide
"fragment", "portion", or "segment" refers to a polynucleotide
sequence of at least about 10 nucleotides and as many as about 60
nucleotides, preferably about 15 to 30 nucleotides, and more
preferably about 20-25 nucleotides.
[0054] "Naturally occurring HIAP3" refers to HIAP3 produced by
human cells that have not been genetically engineered and
specifically contemplates various HIAP3 forms arising from
post-translational modifications of the polypeptide including but
not limited to acetylation, carboxylation, glycosylation,
phosphorylation, lipidation and acylation.
[0055] "Variant(s)" of polynucleotides or polypeptides, as the term
is used herein, are polynucleotides or polypeptides that differ
from a reference polynucleotide or polypeptide, respectively.
Variants in this sense are described below and elsewhere in the
present disclosure in greater detail.
[0056] (1) A polynucleotide that differs in polynucleotide sequence
from another, reference polynucleotide. Generally, differences are
limited so that the polynucleotide sequences of the reference and
the variant are closely similar overall and, in many regions,
identical.
[0057] As noted below, changes in the polynucleotide sequence of
the variant may be silent. That is, they may not alter the amino
acids encoded by the polynucleotide. Where alterations are limited
to silent changes of this type a variant will encode a polypeptide
with the same amino acid sequence as the reference. Also as noted
below, changes in the polynucleotide sequence of the variant may
alter the amino acid sequence of a polypeptide encoded by the
reference polynucleotide. Such polynucleotide changes may result in
amino acid substitutions, additions, deletions, fusions and
truncations in the polypeptide encoded by the reference sequence,
as discussed below.
[0058] (2) A polypeptide that differs in amino acid sequence from
another, reference polypeptide. Generally, differences are limited
so that the sequences of the reference and the variant are closely
similar overall and, in many regions, identical. A variant and
reference polypeptide may differ in amino acid sequence by one or
more substitutions, additions, deletions, fusions and truncations,
which may be present in any combination. Recombinant variants
encoding these same or similar polypeptides may be synthesized or
selected by making use of the "redundancy" in the genetic code.
Various codon substitutions, such as the silent changes which
produce various restriction sites, may be introduced to optimize
cloning into a plasmid or viral vector or expression in a
particular prokaryotic or eukaryotic system. Mutations may also be
introduced to modify the properties of the polypeptide, to change
ligand-binding affinities, interchain affinities, or polypeptide
degradation or turnover rate.
[0059] "Allelic variant" refers to an alternative form of the hiap3
polynucleotide. Alleles result from a mutation, i.e., a change in
the polynucleotide sequence, and generally produce altered mRNAs or
polypeptides whose structure or function may or may not be altered.
Any given gene may have none, one or many allelic forms. Common
mutational changes which give rise to alleles are generally
ascribed to natural deletions, additions or substitutions of
nucleotides. Each of these types of changes may occur alone, or in
combination with the others, or one or more times in a given
sequence.
[0060] "Derivative" refers to polynucleotides or polypeptides
derived from naturally occurring hiap3 or HIAP3, respectively, by
chemical modifications such as ubiquitination, labeling (e.g., with
radionuclides, various enzymatic modifications), pegylation
(derivatization with polyethylene glycol) or by insertion or
substitution of amino acids such as ornithine (or substitution of
the nucleotides which code for such as an amino acid), which do not
normally occur in human proteins.
[0061] "Deletion" is defined as a change in either polynucleotide
or amino acid sequences in which one or more polynucleotides or
amino acid residues, respectively, are absent.
[0062] "Insertion" or "addition" is that change in a polynucleotide
or amino acid sequence which has resulted in the addition of one or
more polynucleotides or amino acid residues, respectively, as
compared to the naturally occurring polynucleotide or amino acid
sequence.
[0063] "Substitution" results from the replacement of one or more
polynucleotides or amino acids by different polynucleotides or
amino acids, respectively.
[0064] Preferably, amino acid substitutions are the result of
replacing one amino acid with another amino acid having similar
structural and/or chemical properties, such as the replacement of a
leucine with an isoleucine or valine, an aspartate with a
glutamate, or a threonine with a serine, i.e. conservative amino
acid replacement. Insertions or deletions are typically in the
range of about 1 to 5 amino acids. The variation allowed may be
experimentally determined by systematically making insertions,
deletions, or substitutions of amino acids in the polypeptide using
recombinant DNA techniques and assaying the resulting recombinant
variants for activity.
[0065] A polypeptide "fragment", "portion", or "segment" is a
stretch of amino acid residues of at least about 5 amino acids,
often at least about 7 amino acids, typically at least about 9 to
13 amino acids, and in various embodiments, at least about 17 or
more amino acids.
[0066] "Recombinant " or "recombinant DNA molecule" refers to a
polynucleotide sequence which is not naturally occurring, or is
made by the artificial combination of two otherwise separated
segments of sequence. By "recombinantly produced" is meant
artificial combination often accomplished by either chemical
synthesis means, or by the artificial manipulation of isolated
segments of polynucleotides, e.g., by genetic engineering
techniques. Such manipulation is usually done to replace a codon
with a redundant codon encoding the same or a conservative amino
acid, while typically introducing or removing a sequence
recognition site. Alternatively, it is performed to join together
polynucleotide segments with desired functions to generate a single
genetic entity comprising a desired combination of functions not
found in the common natural forms. Restriction enzyme recognition
sites, regulation sequences, control sequences, or other useful
features may be incorporated by design. "Recombinant DNA molecules"
include cloning and expression vectors. "Recombinant" may also
refer to a polynucleotide which encodes a polypeptide and is
prepared using recombinant DNA techniques.
[0067] "Isolated" means altered "by the hand of man" from its
natural state; i.e., that, if it occurs in nature, it has been
changed or removed from its original environment, or both. For
example, a naturally occurring polynucleotide or a polypeptide
naturally present in a living animal in its natural state is not
"isolated", but the same polynucleotide or polypeptide separated
from the coexisting materials of its natural state is "isolated",
as the term is employed herein. For example, with respect to
polynucleotides, the term isolated means that it is separated from
the chromosome and cell in which it naturally occurs.
Polynucleotides and polypeptides may occur in a composition, such
as media formulations, solutions for introduction of
polynucleotides or polypeptides, for example, into cells,
compositions or solutions for chemical or enzymatic reactions, for
instance, which are not naturally occurring compositions, and,
therein remain isolated polynucleotides or polypeptides within the
meaning of that term as it is employed herein.
[0068] "Substantially pure" and "substantially homogenous" are used
interchangeably and describe HIAP3 polypeptide, or fragments
thereof, or a DNA segment encoding same, where such polypeptide or
DNA molecule is separated from components that naturally accompany
it. An HIAP3 polypeptide or fragment thereof, or DNA segment
encoding same is substantially free of naturally-associated
components when it is separated from the native contaminants which
accompany it in its natural state. Thus, a polypeptide that is
chemically synthesized or synthesized in a cellular system
different from the cell in which it naturally originates will be
substantially free from its naturally-associated components.
Similarly, a polynucleotide that is chemically synthesized or
synthesized in a cellular system different from the cell in which
it naturally originated will be substantially free from its
naturally-associated components.
[0069] "Homologous", when used to describe a polynucleotide,
indicates that two polynucleotides, or designated sequences
thereof, when optimally aligned and compared, are identical, with
appropriate nucleotide insertions or deletions, in at least 70% of
the nucleotides, usually from about 75% to 99%, and more preferably
at least about 98 to 99% of the nucleotides.
[0070] "Polymerase chain reaction" or "PCR" refers to a procedure
wherein specific pieces of DNA are amplified as described in U.S.
Pat. No. 4,683,195, issued Jul. 28, 1987. Generally, sequence
information from the ends of the polypeptide fragment of interest
or beyond needs to be available, such that oligonucleotide primers
can be designed; these primers will point towards one another, and
will be identical or similar in sequence to opposite strands of the
template to be amplified. The 5' terminal nucleotides of the two
primers will coincide with the ends of the amplified material. PCR
can be used to amplify specific DNA sequences from total genomic
DNA, cDNA transcribed from total cellular RNA, plasmid sequences,
etc. (See generally Mullis et al., Cold Spring Harbor Symp. Quant.
Biol., 51: 263, 1987; Erlich, ed., PCR Technology, Stockton Press,
NY, 1989).
[0071] "Stringency" typically occurs in a range from about T.sub.m
(melting temperature)-5.degree. C. (50.degree. below the T.sub.m of
the probe) to about 20.degree. C. to 25.degree. C. below T.sub.m.
As will be understood by those of skill in the art, a stringency
hybridization can be used to identify or detect identical
polynucleotide sequences or to identify or detect similar or
related polynucleotide sequences. As herein used, the term
"stringent conditions" means hybridization will occur only if there
is at least 95% and preferably at least 97% identity between the
sequences.
[0072] "Hybridization" as used herein, shall include "any process
by which a polynucleotide strand joins with a complementary strand
through base pairing" (Coombs, J., Dictionary of Biotechnology,
Stockton Press, New York, N.Y., 1994).
[0073] "Therapeutically effective dose" refers to that amount of
polypeptide or its antibodies, antagonists, or inhibitors,
including antisense molecules and ribozymes, which ameliorate the
symptoms or conditions of a disease state. Therapeutic efficacy and
toxicity of such compounds can be determined by standard
pharmaceutical procedures in cell cultures or experimental animals,
e.g., ED.sub.50 (the dose therapeutically effective in 50% of the
population) and LD.sub.50 (the dose lethal to 50% of the
population). The dose ratio between therapeutic and toxic effects
is the therapeutic index, and it can be expressed as the ratio,
ED.sub.50/LD.sub.50.
[0074] "Treating" or "treatment" as used herein covers the
treatment of a disease-state in a human patient, which
disease-state is associated with inappropriate apoptosis, and
includes both disease states in which the patient is in need of
decreased levels of HIAP3 and disease states in which the patient
is in need of increased levels of HIAP3.
DETAILED DESCRIPTION OF THE INVENTION
[0075] The present invention relates to novel HIAP3 polypeptides
and hiap3 polynucleotides, among other things, as described in
greater detail below. In particular, the invention relates to novel
HIAP3 polypeptides and the polynucleotides encoding these HIAP3
polypeptides, and relates especially to HIAP3 having the amino acid
sequence set out in FIG. 2 and hiap3 having the polynucleotide
sequence set out in FIG. 1. The present invention also encompasses
HIAP3 variants. A preferred HIAP3 variant is one having at least
70% similarity (preferably at least 70% identity) to the
polypeptide sequence shown in FIG. 2 and more preferably at least
90% similarity (more preferably at least 90% identity) to the
polypeptide shown in FIG. 2 and still more preferably at least 95%
similarity (still more preferably at least 95% identity) to the
polypeptide sequence shown in FIG. 2 and also includes portions of
such polypeptides with such portion of the polypeptide generally
containing at least 30 amino acids and more preferably at least 50
amino acids.
[0076] The polynucleotide sequence encoding a portion of HIAP3 was
first identified as a 226 bp EST (expressed sequence tag; Incyte
Clone #1419118) based on analysis of the Incyte EST database using
the polypeptide consensus sequence of the BIR domain of IAPs and
the BLAST database search program of the Genetic Computer Group
package (Oxford Molecular Group, Campbell, Calif.). The chosen EST
sequence demonstrated a 42% identity with the BIR domain of HIAP1
within a stretch of 57 amino acids. A full length cDNA was obtained
by assembling the sequence of the original EST with sequences
obtained using 3' and 5' RACE (rapid amplification of cDNA end)
technology on a fetal kidney cDNA library and then using the
assemble program of the GCG package. Database searching (both
public and Incyte databases) identified an Incyte EST (Clone
#2953985), containing the 5' end of the polynucleotide sequence.
This clone was obtained and sequenced.
[0077] The cDNA sequence designated hiap3 contains 1337 base pairs.
There are 169 bp in the 5' untranslated region and 274 bp in the 3'
untranslated region flanking the coding region (see FIG. 1). The
coding region consists of 894 nucelotides with an open reading
frame of 298 amino acid residues. The initiation codon ATG is
located at nucelotide position 170. Analysis of the deduced
polypeptide sequence using the ScanProsite feature of the GCG
package reveals a single BIR domain from amino acid position 88 to
155 and a RING zinc finger domain at positions 240 to 289.
[0078] The present invention is based in part on the structural
homology shown in FIGS. 4 and 5 for the BIR and RING domains among
HIAP3 and other members of the IAP protein family. There is
approximately a 50% identity of the BIR domain between HIAP3 and
the third BIR domain of either human or mouse IAP1 or IAP2. There
is a 74% identity of the RING domain between HIAP3 and HIAP1. A
Pile Up analysis shows very high homology within the members of the
family as shown in FIGS. 4 and 5. Northern blot analysis indicated
that the polynucleotide encoding HIAP3 is expressed mainly in
placenta, lymph node, fetal kidney, and kidney tumor, giving it a
tissue pattern distribution different from all other known human
IAPs.
[0079] Polynucleotides
[0080] In accordance with one aspect of the present invention,
there are provided isolated polynucleotides that encode the HIAP3
polypeptide having the deduced amino acid sequence of FIG. 2.
[0081] Using the information provided herein, such as the
polynucleotide sequence set out in FIG. 1, a polynucleotide of the
present invention encoding an HIAP3 polypeptide may be obtained
using standard cloning and screening procedures, such as those for
cloning cDNAs using mRNA from cells of human tissue as starting
material. Illustrative of the invention, the polynucleotide
sequence in FIG. 1 was found in a human fetal kidney cDNA
library.
[0082] Polynucleotides of the present invention may be in the form
of RNA, such as mRNA, or in the form of DNA, including, for
instance, cDNA and genomic DNA obtained by cloning or produced by
chemical synthetic techniques or by a combination thereof. The DNA
may be double-stranded or single-stranded. Single-stranded DNA may
be the coding strand, also known as the sense strand, or it may be
the non-coding strand, also referred to as the anti-sense
strand.
[0083] The coding sequence which encodes the polypeptide may be
identical to the coding sequence of the polynucleotide shown in
FIG. 1. It also may be a polynucleotide with a different sequence,
which, as a result of the redundancy (degeneracy) of the genetic
code, encodes the polypeptide of FIG. 2.
[0084] Polynucleotides of the present invention which encode the
polypeptide of FIG. 2 may include, but are not limited to the
coding sequence for the mature polypeptide, by itself; the coding
sequence for the mature polypeptide and additional coding
sequences, such as those encoding a leader or secretory sequence,
such as a pre-, or pro- or prepro-protein sequence; the coding
sequence of the mature polypeptide, with or without the
aforementioned additional coding sequences, together with
additional, non-coding sequences, including for example, but not
limited to introns and non-coding 5' and 3' sequences, such as the
transcribed, non-translated sequences that play a role in
transcription, mRNA processing--including splicing and
polyadenylation signals, for example--ribosome binding and
stability of mRNA; additional coding sequence which codes for
additional amino acids, such as those which provide additional
functionalities. Thus, for instance, the polypeptide may be fused
to a marker sequence, such as a peptide, which facilitates
purification of the fused polypeptide. In certain preferred
embodiments of this aspect of the invention, the marker sequence is
a hexa-histidine peptide, such as the tag provided in a
pcDNA3.1Myc-His vector (Invitrogen, Carlsbad, Calif.) among others,
many of which are commercially available. As described in Gentz et
al. (Proc. Natl. Acad. Sci., USA 86: 821-824, 1989), for instance,
hexa-histidine provides for convenient purification of the fusion
protein. The HA tag corresponds to an epitope derived from
influenza hemagglutinin protein, which has been described by Wilson
et al. (Cell 37: 767, 1984), for instance.
[0085] The present invention further relates to variants of the
herein above described polynucleotides which encode for fragments,
analogs and derivatives of the polypeptide having the deduced amino
acid sequence of FIG. 2. A variant of the polynucleotide may be a
naturally occurring variant such as a naturally occurring allelic
variant, or it may be a variant that is not known to occur
naturally. Such non-naturally occurring variants of the
polynucleotide may be made by mutagenesis techniques, including
those applied to polynucleotides, cells or organisms.
[0086] Among variants in this regard are variants that differ from
the aforementioned polynucleotides by polynucleotide substitutions,
deletions or additions. The substitutions, deletions or additions
may involve one or more polynucleotides. The variants may be
altered in coding or non-coding regions or both. Alterations in the
coding regions may produce conservative or non-conservative amino
acid substitutions, deletions or additions.
[0087] Among the particularly preferred embodiments of the
invention in this regard are polynucleotides encoding polypeptides
having the amino acid sequence of HIAP3 set out in FIG. 2;
variants, analogs, derivatives and fragments thereof, and fragments
of the variants, analogs and derivatives.
[0088] Further particularly preferred in this regard are
polynucleotides encoding HIAP3 variants, analogs, derivatives and
fragments, and variants, analogs and derivatives of the fragments,
which have the amino acid sequence of the HIAP3 polypeptide of FIG.
2 in which several, a few, 5 to 10, 1 to 5, 1 to 3, 2, 1 or no
amino acid residues are substituted, deleted or added, in any
combination. Especially preferred among these are silent
substitutions, additions and deletions, which do not alter the
properties and activities of the HIAP3 polypeptide. Also especially
preferred in this regard are conservative substitutions. Most
highly preferred are polynucleotides encoding polypeptides having
the amino acid sequence of FIG. 2 without substitutions.
[0089] Further preferred embodiments of the invention are
polynucleotides that are at least 70% identical to a polynucleotide
encoding the HIAP3 polypeptide having the amino acid sequence set
out in FIG. 2, and polynucleotides which are complementary to such
polynucleotides. Alternatively, most highly preferred are
polynucleotides that comprise a region that is at least 80%
identical to a polynucleotide encoding the HIAP3 polypeptide and
polynucleotides complementary thereto. In this regard,
polynucleotides at least 90% identical to the same are particularly
preferred, and among these particularly preferred polynucleotides,
those with at least 95% are especially preferred. Furthermore,
those with at least 97% are highly preferred among those with at
least 95%, and among these, those with at least 98% and at least
99% are particularly highly preferred with at least 99% being the
more preferred.
[0090] Particularly preferred embodiments in this respect,
moreover, are polynucleotides which encode polypeptides which
retain substantially the same biological activity as the mature
polypeptide encoded by the polynucleotide sequence of FIG. 1.
[0091] The present invention further relates to polynucleotides
that hybridize to the herein above-described sequences. In this
regard, the present invention especially relates to polynucleotides
which hybridize under stringent conditions to the herein
above-described polynucleotides. As herein used, the term
"stringent conditions" means hybridization will occur only if there
is at least 95% and preferably at least 97% identity between the
sequences.
[0092] As discussed additionally herein regarding polynucleotide
assays of the invention, for instance, polynucleotides of the
invention as discussed above, may be used as a hybridization probes
for cDNA and genomic DNA to isolate full-length cDNAs and genomic
clones encoding HIAP3 and to isolate cDNA and genomic clones of
other genes that have a high sequence similarity to the hiap3 gene.
Such probes generally will comprise at least 15 bases. Preferably,
such probes will have at least 30 bases and may have at least 50
bases. Particularly preferred probes will have at least 30 bases
and will have 50 bases or less.
[0093] For example, the coding region of the hiap3 gene may be
isolated by screening using the known DNA sequence to synthesize an
oligonucleotide probe. A labeled oligonucleotide having a sequence
complementary to that of a polypeptide of the present invention is
then used to screen a library of human cDNA, genomic DNA or mRNA to
determine to which members of the library the probe hybridizes.
[0094] The polynucleotides and polypeptides of the present
invention may be employed as research reagents and materials for
discovery of treatments and diagnostics to human disease as further
discussed herein relating to polynucleotide assays, inter alia.
[0095] The polynucleotides may encode a polypeptide which is the
mature protein plus additional amino or carboxyl-terminal amino
acids, or amino acids interior to the mature polypeptide (when the
mature form has more than one polypeptide chain, for instance).
Such sequences may play a role in processing of a protein from
precursor to a mature form, may facilitate protein trafficking, may
prolong or shorten protein half-life or may facilitate manipulation
of a protein for assay or production, among other things. As
generally is the case in situ, the additional amino acids may be
processed away from the mature protein by cellular enzymes.
[0096] A precursor protein, having the mature form of the
polypeptide fused to one or more prosequences may be an inactive
form of the polypeptide. When prosequences are removed such
inactive precursors generally are activated. Some or all of the
prosequences may be removed before activation. Generally, such
precursors are called proproteins.
[0097] In sum, a polynucleotide of the present invention may encode
a mature protein, a mature protein plus a leader sequence (which
may be referred to as a preprotein), a precursor of a mature
protein having one or more prosequences which are not the leader
sequences of a preprotein, or a preproprotein, which is a precursor
to a proprotein, having a leader sequence and one or more
prosequences, which generally are removed during processing steps
that produce active and mature forms of the polypeptide.
[0098] Polypeptides
[0099] The present invention further relates to an HIAP3
polypeptide which has the deduced amino acid sequence of FIG.
2.
[0100] The invention also relates to fragments, analogs and
derivatives of these polypeptides. The terms fragment, derivative
and analog when referring to the polypeptide of FIG. 2 means a
polypeptide which retains essentially the same biological activity
as such a polypeptide. Thus, an analog includes a proprotein which
can be activated by cleavage of the proprotein portion to produce
an active mature polypeptide.
[0101] The polypeptide of the present invention may be a
recombinant polypeptide, a natural polypeptide or a synthetic
polypeptide. In certain preferred embodiments it is a recombinant
polypeptide.
[0102] The fragment, derivative or analog of the polypeptide of
FIG. 2 may be (i) one in which one or more of the amino acid
residues are substituted with a conserved or non-conserved amino
acid residue (preferably a conserved amino acid residue) and such
substituted amino acid residue may or may not be one encoded by the
genetic code, or (ii) one in which one or more of the amino acid
residues includes a substituent group, or (iii) one in which the
mature polypeptide is fused with another compound, such as a
compound to increase the half-life of the polypeptide (for example,
polyethylene glycol) or (iv) one in which the additional amino
acids are fused to the mature polypeptide, such as a leader or
secretory sequence or a sequence which is employed for purification
of the mature polypeptide or a proprotein sequence. Such fragments,
derivatives and analogs are deemed to be within the scope of those
skilled in the art from the teachings herein.
[0103] Among the particularly preferred embodiments of the
invention in this regard are polypeptides having the amino acid
sequence of HIAP3 set out in FIG. 2, variants, analogs, derivatives
and fragments thereof, and variants, analogs and derivatives of the
fragments. Alternatively, particularly preferred embodiments of the
invention in this regard are polypeptides having the amino acid
sequence of the HIAP3 shown in FIG. 2, variants, analogs,
derivatives and fragments thereof, and variants, analogs and
derivatives of the fragments.
[0104] Among preferred variants are those that vary from a
reference by conservative amino acid substitutions. Such
substitutions are those that substitute a given amino acid in a
polypeptide by another amino acid of like characteristics.
Typically seen as conservative substitutions are the replacements,
one for another, among the aliphatic amino acids Ala, Val, Leu and
lle, interchange of the hydroxyl residues Ser and Thr, exchange of
the acidic residues Asp and Glu, substitution between the amide
residues Asn and Gln, exchange of the basic residues Lys and Arg
and replacements among the aromatic residues Phe and Tyr.
[0105] Further particularly preferred in this regard are variants,
analogs, derivatives and fragments, and variants, analogs and
derivatives of the fragments, having the amino acid sequence of the
HIAP3 polypeptide of FIG. 2 in which several, a few, 5 to 10, 1 to
5, 1 to 3, 2, 1 or no amino acid residues are substituted, deleted
or added, in any combination. Especially preferred among these are
silent substitutions, additions and deletions, which do not alter
the properties and activities of the HIAP3. Also especially
preferred in this regard are conservative substitutions. Most
highly preferred are polypeptides having the amino acid sequence of
FIG. 2 without substitutions.
[0106] The polypeptides and polynucleotides of the present
invention are preferably provided in an isolated form, and
preferably are purified to homogeneity.
[0107] The polypeptides of the present invention also include the
polypeptide of FIG. 2 (in particular the mature polypeptide) as
well as polypeptides which have at least 70% similarity (preferably
at least 70% identity) to the polypeptide of FIG. 2 and more
preferably at least 90% similarity (more preferably at least 90%
identity) to the polypeptide of FIG. 2 and still more preferably at
least 95% similarity (still more preferably at least 95% identity)
to the polypeptide of FIG. 2 and also include portions of such
polypeptides with such portion of the polypeptide generally
containing at least 30 amino acids and more preferably at least 50
amino acids.
[0108] As known in the art "similarity" between two polypeptides is
determined by comparing the amino acid sequence and its conserved
amino acid substitutes of one polypeptide to the sequence of a
second polypeptide.
[0109] Fragments or portions of the polypeptides of the present
invention may be employed for producing the corresponding
full-length polypeptide by peptide synthesis; therefore, the
fragments may be employed as intermediates for producing the
full-length polypeptides. Fragments or portions of the
polynucleotides of the present invention may be used to synthesize
full-length polynucleotides of the present invention.
[0110] Fragments
[0111] Also among preferred embodiments of this aspect of the
present invention are polypeptides comprising fragments of HIAP3,
most particularly fragments of the HIAP3 having the amino acid
sequence set out in FIG. 2, and fragments of variants and
derivatives of the HIAP3 of FIG. 2.
[0112] In this regard a fragment is a polypeptide having an amino
acid sequence that entirely is the same as part but not all of the
amino acid sequence of the aforementioned HIAP3 polypeptides and
variants or derivatives thereof.
[0113] Such fragments may be "free-standing," i.e., not part of or
fused to other amino acids or polypeptides, or they may be
comprised within a larger polypeptide of which they form a part or
region. When comprised within a larger polypeptide, the presently
discussed fragments most preferably form a single continuous
region. However, several fragments may be comprised within a single
larger polypeptide. For instance, certain preferred embodiments
relate to a fragment of an HIAP3 polypeptide of the present
invention comprised within a precursor polypeptide designed for
expression in a host and having heterologous pre- and
propolypeptide regions fused to the amino terminus of the HIAP3
fragment and an additional region fused to the carboxyl terminus of
the fragment. Therefore, fragments in one aspect of the meaning
intended herein, refers to the portion or portions of a fusion
polypeptide or fusion protein derived from HIAP3.
[0114] As representative examples of polypeptide fragments of the
invention, there may be mentioned those which have from about 25 to
about 145 amino acids.
[0115] In this context about includes the particularly recited
range and ranges larger or smaller by several, a few, 5, 4, 3, 2 or
1 amino acid at either extreme or at both extremes. For instance,
about 145 amino acids in this context means a polypeptide fragment
of 25 plus or minus several, a few, 5, 4, 3, 2 or 1 amino acids to
145 plus or minus several a few, 5, 4, 3, 2 or 1 amino acid
residues, i.e., ranges as broad as 25 minus several amino acids to
145 plus several amino acids to as narrow as 25 plus several amino
acids to 145 minus several amino acids.
[0116] Highly preferred in this regard are the recited ranges plus
or minus as many as 5 amino acids at either or at both extremes.
Particularly highly preferred are the recited ranges plus or minus
as many as 3 amino acids at either or at both the recited extremes.
Especially particularly highly preferred are ranges plus or minus 1
amino acid at either or at both extremes or the recited ranges with
no additions or deletions. Most highly preferred of all in this
regard are fragments from about 25 to about 145 amino acids.
[0117] Among especially preferred fragments of the invention are
truncation mutants of HIAP3. Truncation mutants include HIAP3
polypeptides having the amino acid sequence of FIG. 2, or of
variants or derivatives thereof, except for deletion of a
continuous series of residues (that is, a continuous region, part
or portion) that includes the amino terminus, or a continuous
series of residues that includes the carboxyl terminus or, as in
double truncation mutants, deletion of two continuous series of
residues, one including the amino terminus and one including the
carboxyl terminus. Fragments having the size ranges set out above
also are preferred embodiments of truncation fragments, which are
especially preferred among fragments generally.
[0118] Also preferred in this aspect of the invention are fragments
characterized by structural or functional attributes of HIAP3. Most
preferred are fragments containing the BIR and C-terminal RING zinc
finger domains of HIAP3.
[0119] Certain preferred regions in these regards are set out in
FIG. 3, and include, but are not limited to, regions of the
aforementioned types identified by analysis of the amino acid
sequence set out in FIG. 2. As set out in FIG. 3, such preferred
regions include the BIR domain and the C-terminal RING zinc finger
domain.
[0120] Among highly preferred fragments in this regard are those
that comprise regions of HIAP3 that combine several structural
features, such as several of the features set out above. In this
regard, the BIR and RING domains defined by the amino acid residues
from about 88-155 and from about 240-289, respectively, of FIG. 2,
which are characteristic of the IAP family of proteins are
especially highly preferred regions. Such regions may be comprised
within a larger polypeptide or may be by themselves a preferred
fragment of the present invention, as discussed above. It will be
appreciated that the term "about" as used in this paragraph has the
meaning set out above regarding fragments in general.
[0121] Further preferred regions are those that mediate activities
of HIAP3. Most highly preferred in this regard are fragments that
have a chemical, biological or other activity of HIAP3, including
those with a similar activity or an improved activity, or with a
decreased undesirable activity. Highly preferred in this regard are
fragments that contain regions that are homologs in sequence, or in
position, or in both sequence and position to active regions of
related polypeptides, such as the other proteins of the IAP family,
which includes HIAP3.
[0122] It will be appreciated that the invention also relates to,
among others, polynucleotides encoding the aforementioned
fragments, polynucleotides that hybridize to polynucleotides
encoding the fragments, particularly those that hybridize under
stringent conditions, and polynucleotides, such as PCR primers, for
amplifying polynucleotides that encode the fragments. In these
regards, preferred polynucleotides are those that correspond to the
preferred fragments, as discussed above.
[0123] Vectors, Host Cells, and Expression Systems
[0124] The present invention also relates to vectors which include
polynucleotides of the present invention, host cells which are
genetically engineered with vectors of the invention and the
production of polypeptides of the invention by recombinant
techniques. Such techniques are described in Sambrook et al.,
Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press,
Plainview, N.Y., 1989 and Ausubel, F. M. et al., Current Protocols
in Molecular Biology, John Wiley & Sons, New York, N.Y.,
1989.
[0125] Host cells can be genetically engineered to incorporate
polynucleotides and express polypeptides of the present invention.
For instance, polynucleotides may be introduced into host cells
using well known techniques of infection, transduction,
transfection, transvection and transformation. The polynucleotides
may be introduced alone or with other polynucleotides. Such other
polynucleotides may be introduced independently, co-introduced or
introduced joined to the polynucleotides of the invention.
[0126] Thus, for instance, polynucleotides of the invention may be
transfected into host cells with another, separate, polynucleotide
encoding a selectable marker, using standard techniques for
co-transfection and selection in, for instance, mammalian cells. In
this case, the polynucleotides generally will be stably
incorporated into the host cell genome.
[0127] Alternatively, the polynucleotides may be joined to a vector
containing a selectable marker for propagation in a host. The
vector construct may be introduced into host cells by the
aforementioned techniques. Generally, a plasmid vector is
introduced as DNA in a precipitate, such as a calcium phosphate
precipitate, or in a complex with a charged lipid. Electroporation
also may be used to introduce polynucleotides into a host. If the
vector is a virus, it may be packaged in vitro or introduced into a
packaging cell and the packaged virus may be transduced into cells.
A wide variety of techniques suitable for making polynucleotides
and for introducing polynucleotides into cells in accordance with
this aspect of the invention are well known and routine to those of
skill in the art. Such techniques are reviewed at length in
Sambrook et al. cited above, which is illustrative of the many
laboratory manuals that detail these techniques. In accordance with
this aspect of the invention, the vector may be, for example, a
plasmid vector, a single or double-stranded phage vector, a single
or double-stranded RNA or DNA viral vector. Such vectors may be
introduced into cells as polynucleotides, preferably DNA, by well
known techniques for introducing DNA and RNA into cells. The
vectors, in the case of phage and viral vectors, also may be and
preferably are introduced into cells as packaged or encapsidated
virus by well known techniques for infection and transduction.
Viral vectors may be replication competent or replication
defective. In the latter case viral propagation generally will
occur only in complementing host cells.
[0128] Preferred among vectors, in certain respects, are those for
expression of polynucleotides and polypeptides of the present
invention. Generally, such vectors comprise cis-acting control
regions effective for expression in a host operatively linked to
the polynucleotide to be expressed. Appropriate trans-acting
factors either are supplied by the host, supplied by a
complementing vector or supplied by the vector itself upon
introduction into the host.
[0129] In certain preferred embodiments in this regard, the vectors
provide for specific expression. Such specific expression may be
inducible expression or expression only in certain types of cells
or both inducible and cell-specific. Particularly preferred among
inducible vectors are vectors that can be induced for expression by
environmental factors that are easy to manipulate, such as
temperature and nutrient additives. A variety of vectors suitable
to this aspect of the invention, including constitutive and
inducible expression vectors for use in prokaryotic and eukaryotic
hosts, are well known and employed routinely by those of skill in
the art.
[0130] The engineered host cells can be cultured in conventional
nutrient media, which may be modified as appropriate for, inter
alia, activating promoters, selecting transformants or amplifying
genes. Culture conditions, such as temperature, pH and the like,
previously used with the host cell selected for expression
generally will be suitable for expression of polypeptides of the
present invention as will be apparent to those of skill in the
art.
[0131] A great variety of expression vectors can be used to express
a polypeptide of the invention. Such vectors include chromosomal,
episomal and virus-derived vectors e.g., vectors derived from
bacterial plasmids, from bacteriophage, from yeast episomes, from
yeast chromosomal elements, from viruses such as baculoviruses,
papova viruses, such as SV4O, vaccinia viruses, adenoviruses, fowl
pox viruses, pseudorabies viruses and retroviruses, and vectors
derived from combinations thereof, such as those derived from
plasmid and bacteriophage genetic elements, such as cosmids and
phagemids, all may be used for expression in accordance with this
aspect of the present invention. Generally, any vector suitable to
maintain, propagate or express polynucleotides to express a
polypeptide in a host may be used for expression in this
regard.
[0132] The appropriate DNA sequence may be inserted into the vector
by any of a variety of well-known and routine techniques. In
general, a DNA sequence for expression is joined to an expression
vector by cleaving the DNA sequence and the expression vector with
one or more restriction endonucleases and then joining the
restriction fragments together using T4 DNA ligase. Procedures for
restriction and ligation that can be used to this end are well
known and routine to those of skill. Suitable procedures in this
regard, and for constructing expression vectors using alternative
techniques, which also are well known and routine to those of
skill, are set forth in great detail in Sambrook et al. cited
elsewhere herein.
[0133] The DNA sequence in the expression vector is operatively
linked to appropriate expression control sequence(s), including,
for instance, a promoter to direct mRNA transcription.
Representatives of such promoters include the phage lambda PL
promoter, the E. coli lac, trp and tac promoters, the SV4O early
and late promoters and promoters of retroviral LTRs, to name just a
few of the well-known promoters. It will be understood that
numerous promoters not mentioned are suitable for use in this
aspect of the invention, are well known and may readily be employed
by those of skill in the manner illustrated by the discussion and
the examples herein.
[0134] In general, expression constructs will contain sites for
transcription initiation and termination and, in the transcribed
region, a ribosome binding site for translation. The coding portion
of the mature transcripts expressed by the constructs will include
a translation initiating AUG at the beginning and a termination
codon appropriately positioned at the end of the polypeptide to be
translated.
[0135] In addition, the constructs may contain control regions that
regulate as well as engender expression. Generally, in accordance
with many commonly practiced procedures, such regions will operate
by controlling transcription, such as repressor binding sites and
enhancers, among others.
[0136] Vectors for propagation and expression generally will
include selectable markers. Such markers also may be suitable for
amplification or the vectors may contain additional markers for
this purpose. In this regard, the expression vectors preferably
contain one or more selectable marker genes to provide a phenotypic
trait for selection of transformed host cells. Preferred markers
include dihydrofolate reductase or neomycin resistance for
eukaryotic cell culture, and tetracycline, theomycin, kanamycin or
ampicillin resistance genes for culturing E. coli and other
bacteria.
[0137] The vector containing the appropriate DNA sequence as
described elsewhere herein, as well as an appropriate promoter, and
other appropriate control sequences, may be introduced into an
appropriate host using a variety of well known techniques suitable
to expression therein of a desired polypeptide. Representative
examples of appropriate hosts include bacterial cells, such as E.
coli. Streptomyces and Salmonella typhimurium cells; fungal cells,
such as yeast cells; insect cells such as Drosophila 52 and
Spodoptera Sf9 cells; animal cells such as CHO, COS and Bowes
melanoma cells; and plant cells. Hosts for a great variety of
expression constructs are well known, and those of skill will be
enabled by the present disclosure readily to select a host for
expressing a polypeptides in accordance with this aspect of the
present invention.
[0138] Various mammalian cell culture systems can be employed for
expression, as well. Examples of mammalian expression systems
include the COS-7 lines of monkey kidney fibroblast (Gluzman et
al., Cell 23: 175, 1991). Other cell lines capable of expressing a
compatible vector include for example, the C127, 3T3, CHO, HeLa,
human kidney 293 and BHK cell lines. In mammalian host cells, a
number of viral-based expression systems may be utilized. In cases
where an adenovirus is used as an expression vector, the
polynucleotide sequence coding for HIAP3 may be ligated into an
adenovirus transcription/translation complex consisting of the late
promoter and tripartite leader sequence. Insertion in a
nonessential E1 or E3 region of the viral genome will result in a
viable virus capable of expressing HIAP3 in infected host cells
(Logan and Shenk, Proc. Natl. Acad. Sci. USA 81:3655-59, 1984). In
addition, transcription enhancers, such as the rouse sarcoma virus
(RVS) enhancer, may be used to increase expression in mammalian
host cells.
[0139] More particularly, the present invention also includes
recombinant constructs, such as expression constructs, comprising
one or more of the sequences described above. The constructs
comprise a vector, such as a plasmid or viral vector, into which
such a sequence of the invention has been inserted. The sequence
may be inserted in a forward or reverse orientation. In certain
preferred embodiments in this regard, the construct further
comprises regulatory sequences, including, for example, a promoter,
operably linked to the sequence. Large numbers of suitable vectors
and promoters are known to those of skill in the art, and there are
many commercially available vectors suitable for use in the present
invention.
[0140] The following vectors, which are commercially available, are
provided by way of example. Among vectors preferred for use in
bacteria are pQE7O, pQE6O and pQE-9, available from Qiagen USA
(Valencia, Calif.); pBS vectors, Phagescript.RTM. vectors,
Bluescript.RTM. vectors, pNH8A, pNHI6a, pNHI8A, pNH46A, available
from Stratagene (LaJolla, Calif.); and ptrc99a, pK223-3, pKK233-3,
pDR54O, pRIT5 available from Pharmacia Biotech (Piscataway, N.J.).
Most preferred is the pGEX-6P-3 vector, available from Pharmacia
Biotech. Among preferred eukaryotic vectors are pWLNEO, pSV2CAT,
pOG44, PXTI and pSG available from Stratagene; and PSVK3, pBPV,
pMSG and pSVL available from Pharmacia Biotech. Most preferred are
the vectors pcDNA3.1 Myc-His and pRc/CMV2 available from Introgene.
These vectors are listed solely by way of illustration of the many
commercially available and well known vectors that are available to
those of skill in the art for use in accordance with this aspect of
the present invention. It will be appreciated that any other
plasmid or vector suitable for, for example, introduction,
maintenance, propagation or expression of a polynucleotide or
polypeptide of the invention in a host may be used in this aspect
of the invention.
[0141] Promoter regions can be selected from any desired gene using
vectors that contain a reporter transcription unit lacking a
promoter region, such as a chloramphenicol acetyl transferase
("cat") transcription unit, downstream of restriction site or sites
for introducing a candidate promoter fragment; i.e., a fragment
that may contain a promoter. As is well known, introduction into
the vector of a promoter-containing fragment at the restriction
site upstream of the cat gene engenders production of CAT activity,
which can be detected by standard CAT assays. Vectors suitable to
this end are well known and readily available. Two such vectors are
pKK232-B and pCM7. Thus, promoters for expression of
polynucleotides of the present invention include not only well
known and readily available promoters, but also promoters that
readily may be obtained by the foregoing technique, using a
reporter gene.
[0142] Among known bacterial promoters suitable for expression of
polynucleotides and polypeptides in accordance with the present
invention are the E. coli lacI and lacZ promoters, the T3 and T7
promoters, the T5 tac promoter, the lambda PR, PL promoters and the
trp promoter. Among known eukaryotic promoters suitable in this
regard are the CMV immediate early promoter, the HSV thymidine
kinase promoter, the early and late SV4O promoters, the promoters
of retroviral LTRs, such as those of the Rous sarcoma virus ("RSV")
and metallothionein promoters, such as the mouse metallothionein- I
promoter.
[0143] Selection of appropriate vectors and promoters for
expression in a host cell is a well known procedure and the
requisite techniques for expression vector construction,
introduction of the vector into the host and expression in the host
are routine skills in the art.
[0144] Generally, recombinant expression vectors will include
origins of replication, a promoter derived from a highly-expressed
gene to direct transcription of a downstream structural sequence,
and a selectable marker to permit isolation of vector containing
cells after exposure to the vector.
[0145] The present invention also relates to host cells containing
the above-described constructs discussed above. The host cell can
be a higher eukaryotic cell, such as a mammalian cell, or a lower
eukaryotic cell, such as a yeast cell, or the host cell can be a
prokaryotic cell, such as a bacterial cell. Constructs in host
cells can be used in a conventional manner to produce the gene
product encoded by the recombinant sequence. Alternatively, the
polypeptides of the present invention can be produced by direct
peptide synthesis using solid-phase techniques (Stewart et al.,
Solid-Phase Peptide Synthesis, W. H. Freeman Co., San Francisco,
1969; Merrifield, J., J. Am. Chem. Soc. 85:2149-2154, 1963). In
vitro protein synthesis may be performed using manual techniques or
by automation. Automated synthesis may be achieved, for example,
using Applied Biosystems 431A Peptide Synthesizer (Perkin Elmer,
Foster City, Calif.) in accordance with the instructions provided
by the manufacturer. Various fragments of HIAP3 may be chemically
synthesized separately and combined using chemical methods to
produce the full length molecule.
[0146] Mature proteins can be expressed in mammalian cells, yeast,
bacteria, or other cells under the control of appropriate
promoters. Cell-free translation systems can also be employed to
produce such proteins using RNAs derived from the DNA constructs of
the present invention. Appropriate cloning and expression vectors
for use with prokaryotic and eukaryotic hosts are described by
Sambrook et al., cited elsewhere herein.
[0147] Transcription of the DNA encoding the polypeptides of the
present invention by higher eukaryotes may be increased by
inserting an enhancer sequence into the vector. Enhancers are
cis-acting elements of DNA, usually about from 10 to 300 bp that
act to increase transcriptional activity of a promoter in a given
host cell-type. Examples of enhancers include the SV4O enhancer,
which is located on the late side of the replication origin at bp
100 to 270, the cytomegalovirus early promoter enhancer, the
polyoma enhancer on the late side of the replication origin, and
adenovirus enhancers.
[0148] Polynucleotides of the invention, encoding the heterologous
structural sequence of a polypeptide of the invention generally
will be inserted into the vector using standard techniques so that
it is operably linked to the promoter for expression. The
polynucleotide will be positioned so that the transcription start
site is located appropriately 5' to a ribosome binding site. The
ribosome binding site will be 5' to the AUG that initiates
translation of the polypeptide to be expressed. Generally, there
will be no other open reading frames that begin with an initiation
codon, usually AUG, and lie between the ribosome binding site and
the initiating AUG. Also, generally, there will be a translation
stop codon at the end of the polypeptide and there will be a
polyadenylation signal and a transcription termination signal
appropriately disposed at the 3' end of the transcribed region.
[0149] For secretion of the translated protein into the lumen of
the endoplasmic reticulum, into the periplasmic space or into the
extracellular environment, appropriate secretion signals may be
incorporated into the expressed polypeptide. The signals may be
endogenous to the polypeptide or they may be heterologous signals.
The polypeptide may be expressed in a modified form, such as a
fusion protein, and may include not only secretion signals but also
additional heterologous fuctional regions. Thus for instance, a
region of additional amino acids, particularly charged amino acids,
may be added to the N-terminus of the polypeptide to improve
stability and persistence in the host cell, during purification or
during subsequent handling and storage. Also, special regions also
may be added to the polypeptide to facilitate purification. Such
regions may be removed prior to final preparation of the
polypeptide. The addition of peptide moieties to polypeptides to
engender secretion or excretion, to improve stability and to
facilitate purification, among others, are familiar and routine
techniques in the art. For example, when large quantities of HIAP3
are needed for the induction of antibodies, vectors which direct
high level expression of fusion proteins that are readily purified
may be desirable. Such vectors include, but are not limited to, the
multifunctional E. coli cloning and expression vectors such as
Bluescript.RTM. (Stratagene), in which the hiap3 coding sequence
may be ligated into the vector in frame with sequence for the
amino-terminal Met and the subsequent 7 residues of
.beta.-galactosidase so that a hybrid protein is produced; pIN
vectors (Van Heede and Shuster, J. Biol. Chem. 264:5503-5509, 1989)
and the like. PGEX vectors (Promega, Madison, Wis.) may also be
used to express foreign polypeptides as fusion proteins with
glutathione S-transferase (GST). In general, such fusion proteins
are soluble and can easily be purified from lysed cells by
adsorption to glutathione-agarose beads followed by elution in the
presence of free glutathione. Proteins made in such systems are
designed to include heparin, thrombin or factor Xa protease
cleavage sites so that the cloned polypeptide of interest can be
released from the GST moiety at will.
[0150] Following transformation of a suitable host strain and
growth of the host strain to an appropriate cell density, where the
selected promoter is inducible it is induced by appropriate means
(e.g., temperature shift or exposure to chemical inducer) and cells
are cultured for an additional period.
[0151] Cells typically then are harvested by centrifugation,
disrupted by physical or chemical means, and the resulting crude
extract retained for further purification.
[0152] Microbial cells employed in expression of proteins can be
disrupted by any convenient method, including freeze-thaw cycling,
sonication, mechanical disruption, or use of cell lysing agents,
such methods are well know to those skilled in the art.
[0153] The HIAP3 polypeptide can be recovered and purified from
recombinant cell cultures by well-known methods including ammonium
sulfate or ethanol precipitation, acid extraction, anion or cation
exchange chromatography, phosphocellulose chromatography,
hydrophobic interaction chromatography, affinity chromatography,
hydroxylapatite chromatography and lectin chromatography. Most
preferably, high performance liquid chromatography ("HPLC") is
employed for purification. Well known techniques for refolding
protein may be employed to regenerate active conformation when the
polypeptide is denatured during isolation and or purification.
Various other methods of protein purification well known in the art
include those described in Deutscher, M., Methods in Enzymology,
Vol 182, Academic Press, San Diego, 1982; and Scopes, R., Protein
Purification: Principles and Practice Springer-Verlag, New York,
1982.
[0154] Polypeptides of the present invention include naturally
purified products, products of chemical synthetic procedures, and
products produced by recombinant techniques from a prokaryotic or
eukaryotic host, including, for example, bacterial, yeast, higher
plant, insect and mammalian cells. Depending upon the host employed
in a recombinant production procedure, the polypeptides of the
present invention may be glycosylated or may be non-glycosylated.
In addition, polypeptides of the invention may also include an
initial modified methionine residue, in some cases as a result of
host-mediated processes.
[0155] Uses of HIAP3 Polypeptides and the Polynucleotides which
Encode Them
[0156] hiap3 polynucleotides and HIAP3 polypeptides may be used in
accordance with the present invention for a variety of
applications, particularly those that make use of the chemical and
biological properties of HIAP3. Additional applications relate to
diagnosis and to treatment of disorders of cells, tissues and
organisms related to the presence of inappropriate apoptosis. These
aspects of the invention are illustrated further by the following
discussion and are described further within the body of the
specification.
[0157] The rationale for the use of the polynucleotide and
polypeptide sequences of the present invention is based in part on
the chemical and structural homology between the HIAP3 disclosed
herein and other IAP molecules. HIAP3 may be used in the diagnosis
and treatment of conditions, disorders or diseases associated with
abnormal or inappropriate apoptosis. These would include, but are
not limited to, cancer and chronic viral infections, in which
apoptosis is insufficient, and neurodegenerative disorders and
chronic heart failure, which are characterized by premature
apoptosis.
[0158] hiap3 polynucleotide sequences can be used for chromosome
identification and as DNA probes.
[0159] HIAP3 polypeptides can be used to generate antibodies to
HIAP3 which may be useful in detecting the levels of HIAP3 protein
in cells and tissues.
[0160] Polynucleotides encoding HIAP3 may be useful in diagnostic
assays for detecting the levels of polynucleotides encoding HIAP3
in cells and tissues.
[0161] In conditions associated with overexpression of HIAP3, such
as cancer, it may be advantageous to suppress HIAP3 production,
thus reducing HIAP3 levels. HIAP3 could be suppressed by
administration of antisense oligonucleotides or ribozymes.
Alternatively, antibodies specifically recognizing areas of the
HIAP3 polypeptide which are responsible for its activity may be
introduced to treat diseases or conditions associated with abnormal
HIAP3 activity.
[0162] In conditions associated with underexpression of HIAP3, such
as neurodegenerative disorders, it may be advantageous to increase
HIAP3 levels. HIAP3 could be supplied to the patient using gene
therapy, in which expression of HIAP3 polypeptide occurs in vivo
within the patient following administration of suitable recombinant
molecules containing polynucleotides encoding and expressing HIAP3
polypeptide.
[0163] Polynucleotide Assays
[0164] This invention is also related to the use of the HIAP3
polynucleotides to detect complementary polynucleotides such as,
for example, as a diagnostic reagent. Detection of a mutated form
of HIAP3 associated with a dysfunction will provide a diagnostic
tool that can add or define a diagnosis of a disease or
susceptibility to a disease which results from under-expression,
over-expression or altered expression of HIAP3, such as, for
example, neoplasia and neurodegenerative disorders.
[0165] Individuals carrying mutations in the gene encoding HIAP3
may be detected at the DNA level by a variety of techniques.
Polynucleotide samples for diagnosis may be obtained from a
patient's cells, such as from blood, urine, saliva, tissue biopsy
and autopsy material. The genomic DNA may be used directly for
detection or may be amplified enzymatically by using PCR prior to
analysis (Saiki et al., Nature, 324: 163-166, 1986). RNA or cDNA
may also be used in the same ways. As an example, PCR primers
complementary to the polynucleotide sequence encoding HIAP3 can be
used to identify and analyze hiap3 expression and mutations. For
example, deletions and insertions can be detected by a change in
size of the amplified product in comparison to the normal genotype.
Point mutations can be identified by hybridizing amplified DNA to
radiolabeled hiap3 RNA or alternatively, radiolabeled hiap3
antisense DNA sequences. Perfectly matched sequences can be
distinguished from mismatched duplexes by RNase A digestion or by
differences in melting temperatures.
[0166] Sequence differences between a reference gene and genes
having mutations also may be revealed by direct DNA sequencing. In
addition, cloned DNA segments may be employed as probes to detect
specific DNA segments. The sensitivity of such methods can be
greatly enhanced by appropriate use of PCR or another amplification
method. For example, a sequencing primer is used with
double-stranded PCR product or a single-stranded template molecule
generated by a modified PCR. The sequence determination is
performed by conventional procedures with radiolabeled
polynucleotide or by automatic sequencing procedures with
fluorescent tags.
[0167] Genetic testing based on DNA sequence differences may be
achieved by detection of alteration in electrophoretic mobility of
DNA fragments in gels, with or without denaturing agents. Small
sequence deletions and insertions can be visualized by high
resolution gel electrophoresis. DNA fragments of different
sequences may be distinguished on denaturing formamide gradient
gels in which the mobilities of different DNA fragments are
retarded in the gel at different positions according to their
specific melting or partial melting temperatures (see, e.g., Myers
et al., Science, 230: 1242, 1985).
[0168] Sequence changes at specific locations also may be revealed
by nuclease protection assays, such as RNase and S1 protection or
the chemical cleavage method (e.g., Catton et al., Proc. Natl.
Acad. Sci., USA, 85:4397-4401, 1985).
[0169] Thus, the detection of a specific DNA sequence may be
achieved by methods such as hybridization, RNase protection,
chemical cleavage, direct DNA sequencing or the use of restriction
enzymes, (e.g., restriction fragment length polymorphisms ("RFLP")
and Southern blotting of genomic DNA).
[0170] In addition to more conventional gel-electrophoresis and DNA
sequencing, mutations also can be detected by in situ analysis.
[0171] Chromosome Assays
[0172] The polynucleotide sequences of the present invention are
also valuable for chromosome identification. The sequence is
specifically targeted to and can hybridize with a particular
location on an individual human chromosome. Moreover, there is a
current need for identifying particular sites on the chromosome.
Few chromosome marking reagents based on actual sequence data
(repeat polymorphisms) are presently available for marking
chromosomal location. The mapping of DNAs to chromosomes according
to the present invention is an important first step in correlating
those sequences with genes associated with disease.
[0173] In certain preferred embodiments in this regard, the cDNA
herein disclosed is used to clone genomic DNA of an hiap3 gene.
This can be accomplished using a variety of well known techniques
and libraries, which generally are available commercially. The
genomic DNA then is used for in situ chromosome mapping using well
known techniques for this purpose. Typically, in accordance with
routine procedures for chromosome mapping, some trial and error may
be necessary to identify a genomic probe that gives a good in situ
hybridization signal.
[0174] In some cases, in addition, sequences can be mapped to
chromosomes by preparing PCR primers (preferably 15-25 bp) from the
cDNA. Computer analysis of the 3' untranslated region of the gene
is used to rapidly select primers that do not span more than one
exon in the genomic DNA, thus complicating the amplification
process. These primers are then used for PCR screening of somatic
cell hybrids containing individual human chromosomes. Only those
hybrids containing the human gene corresponding to the primer will
yield an amplified fragment.
[0175] PCR mapping of somatic cell hybrids is a rapid procedure for
assigning a particular DNA to a particular chromosome. Using the
present invention with the same oligonucleotide primers,
sublocalization can be achieved with panels of fragments from
specific chromosomes or pools of large genomic clones in an
analogous manner. Other mapping strategies that can similarly be
used to map to its chromosome include in situ hybridization,
prescreening with labeled flow-sorted chromosomes and preselection
by hybridization to construct chromosome specific-cDNA
libraries.
[0176] Fluorescence in situ hybridization ("FISH") of a cDNA clone
to a metaphase chromosomal spread can be used to provide a precise
chromosomal location in one step. This technique can be used with
cDNA as short as 50 or 60 bases. For a review of this technique,
see Verma et al., Human Chromosomes: A Manual of Basic Techniques,
Pergamon Press, New York, 1988.
[0177] Once a sequence has been mapped to a precise chromosomal
location, the physical position of the sequence on the chromosome
can be correlated with genetic map data. Such data are found, for
example, in V. McKusick, Mendelian Inheritance in Man, available on
line through Johns Hopkins University, Welch Medical Library. The
relationship between genes and diseases that have been mapped to
the same chromosomal region are then identified through linkage
analysis (coinheritance of physically adjacent genes).
[0178] Next, it is necessary to determine the differences in the
cDNA or genomic sequence between affected and unaffected
individuals. If a mutation is observed in some or all of the
affected individuals but not in any normal individuals, then the
mutation is likely to be the causative agent of the disease.
[0179] With current resolution of physical mapping and genetic
mapping techniques, a cDNA precisely localized to a chromosomal
region associated with the disease could be one of between 50 and
500 potential causative genes. (This assumes 1 megabase mapping
resolution and one gene per 20 kb). In situ hybridization of
chromosomal preparations and physical mapping techniques such as
linkage analysis using established chromosomal markers may be used
for extending genetic maps. For example, an STS based map of the
human genome was published by the Whitehead-MIT Center for Genomic
Research (Hudson et al., Science 270:1945-1954, 1995). Often the
placement of a gene on the chromosome of another mammalian species
such as mouse (Whitehead Institute/MIT Center for Genome Research,
Genetic Map of the Mouse, Database Release 10, Apr. 28, 1995) may
reveal associated markers even if the number or arm of a particular
human chromosome is not known. New sequences can be assigned to
chromosomal arms, or parts thereof, by physical mapping. This
provides valuable information to investigators searching for
disease genes using positional cloning or other gene discovery
techniques. Once a disease or syndrome, such as ataxia
telangiectasia (AT) has been crudely localized by genetic linkage
to a particular genomic region, for example, AT to 11q22-23 (Gatti
et al., Nature 336:577-580, 1988), any sequences mapping to that
area may represent associated or regulatory genes for further
investigation. The nucleotide sequence of the present invention may
also be used to detect differences in the chromosomal location due
to translocation, inversion, etc. among normal, carrier or affected
individuals.
[0180] Polypeptide Assays
[0181] The present invention also relates to a diagnostic assays
such as quantitative and diagnostic assays for detecting levels of
HIAP3 protein in cells and tissues, including determination of
normal and abnormal levels. Thus, for instance, a diagnostic assay
in accordance with the invention for detecting over-expression of
HIAP3 protein compared to normal control tissue samples may be used
to detect the presence of neoplasia, for example, tumors. Assay
techniques that can be used to determine levels of a protein, such
as an HIAP3 protein of the present invention, in a sample derived
from a host are well-known to those of skill in the art. Such assay
methods include radioimmunoassays (RIA), competitive-binding
assays, western Blot analysis and enzyme-linked immunoabsorbant
assays (ELISA), and fluorescent activated cell sorting (FACS).
Among these ELISAs frequently are preferred. An ELISA assay
initially comprises preparing an antibody specific to HIAP3,
preferably a monoclonal antibody. In addition a reporter antibody
generally is prepared which binds to the monoclonal antibody. The
reporter antibody is attached to a detectable reagent such as a
radioactive, fluorescent or enzymatic reagent, in this example
horseradish peroxidase enzyme.
[0182] To carry out an ELISA a sample is removed from a host and
incubated on a solid support, e.g. a polystyrene dish, that binds
the proteins in the sample. Any free protein binding sites on the
dish are then covered by incubating with a non-specific protein
such as bovine serum albumin. Next, the monoclonal antibody is
incubated in the dish during which time the monoclonal antibodies
attach to any HIAP3 proteins attached to the polystyrene dish.
Unbound monoclonal antibody is washed out with buffer. The reporter
antibody linked to horseradish peroxidase is placed in the dish
resulting in binding of the reporter antibody to any monoclonal
antibody bound to HIAP3. Unattached reporter antibody is then
washed out. Reagents for peroxidase activity, including a
colorimetric substrate are then added to the dish. Immobilized
peroxidase, linked to HIAP3 through the primary and secondary
antibodies, produces a colored reaction product. The amount of
color developed in a given time period indicates the amount of
HIAP3 protein present in the sample. Quantitative results typically
are obtained by reference to a standard curve.
[0183] A competition assay may be employed wherein antibodies
specific to HIAP3 are attached to a solid support and labeled HIAP3
and a sample derived from the host are passed over the solid
support and the amount of label detected attached to the solid
support can be correlated to a quantity of HIAP3 in the sample.
[0184] These and other assays are described, among other places, in
Hampton et al. (Serological Methods, a Laboratory Manual, APS
Press, St Paul, Minn., 1990) and Maddox et al. (J. Exp. Med.
158:12111, 1983).
[0185] Antibodies
[0186] The polypeptides, their fragments or other derivatives, or
analogs thereof, or cells expressing them can be used as an
immunogen to produce antibodies thereto. These antibodies can be,
for example, polyclonal or monoclonal antibodies. The present
invention also includes chimeric, single chain, and humanized
antibodies, as well as Fab fragments, or the product of an Fab
expression library. Various procedures known in the art may be used
for the production of such antibodies and fragments.
[0187] Antibodies generated against the polypeptides corresponding
to a sequence of the present invention can be obtained by direct
injection of the polypeptides into an animal or by administering
the polypeptides to an animal, preferably a nonhuman. The antibody
so obtained will then bind the polypeptides itself. In this manner
even a sequence encoding only a fragment of the polypeptides can be
used to generate antibodies binding the whole native polypeptides.
Such antibodies can then be used to isolate the polypeptide from
tissue expressing that polypeptide.
[0188] For preparation of monoclonal antibodies, any technique
which provides antibodies produced by continuous cell line cultures
can be used. Examples include the hybridoma technique (Kohler and
Milstein, Nature 256: 495-497, 1975), the human B-cell hybridoma
technique (Kozbor et al., Immunology Today 4:72, 1983) and the
EBV-hybridoma technique to produce human monoclonal antibodies
(Cole et al., in Monoclonal Antibodies and Cancer, Alan R. Liss,
Inc., 77-96, 1985).
[0189] In addition, techniques developed for the production of
"chimeric antibodies", the splicing of mouse antibody genes to
human antibody genes to obtain a molecule with appropriate antigen
specificity and biological activity can be used (Morrison et al.,
Proc. Natl. Acad. Sci. USA 81:6851-6855, 1984; Neuberger et al.,
Nature 312:604-608, 1984; Takeda et al., Nature 314:452-454, 1985).
Alternatively, techniques described for the production of single
chain antibodies (U.S. Pat. No. 4,946,778) can be adapted to
produce HIAP3-specific single chain antibodies.
[0190] Antibodies may also be produced by inducing in vivo
production in the lymphocyte population or by screening recombinant
immunoglobulin libraries or panels of highly specific binding
reagents as disclosed in Orlandi et al. (Proc. Natl. Acad. Sci. USA
86:3833-3837, 1989) and Winter and Milstein (Nature 349:293-299,
1991).
[0191] Antibody fragments which contain specific binding sites for
HIAP3 may also be generated. For example, such fragments include,
but are not limited to the F(ab')2 fragments which can be produced
by pepsin digestion of the antibody molecule and the Fab fragments
which can be generated by reducing the disulfide bridges of the
F(ab')2 fragments. Alternatively, Fab expression libraries may be
constructed to allow rapid and easy identification of monoclonal
Fab fragments with the desired specificity (Huse et al., Science
256:1270-1281, 1989).
[0192] The above-described antibodies may be employed to isolate or
to identify clones expressing the polypeptide or purify the
polypeptide of the present invention by attachment of the antibody
to a solid support for isolation and/or purification by affinity
chromatography.
[0193] Pharmaceutical Compositions and Administration
[0194] The present invention also relates to pharmaceutical
compositions which may comprise hiap3 polynucleotides, HIAP3
proteins, antibodies, agonists, antagonists, or inhibitors, alone
or in combination with at least one other agent, such as
stabilizing compound, which may be administered in any sterile,
biocompatible pharmaceutical carrier, including, but not limited
to, saline, buffered saline, dextrose, and water. Any of these
molecules can be administered to a patient alone, or in combination
with other agents, drugs or hormones, in pharmaceutical
compositions where it is mixed with excipient(s) or
pharmaceutically acceptable carriers. In one embodiment of the
present invention, the pharmaceutically acceptable carrier is
pharmaceutically inert.
[0195] The present invention also relates to the administration of
pharmaceutical compositions. Such administration is accomplished
orally or parenterally. Methods of parenteral delivery include
topical, intra-arterial (directly to the tumor), intramuscular,
subcutaneous, intramedullary, intrathecal, intraventricular,
intravenous, intraperitoneal, or intranasal administration. In
addition to the active ingredients, these pharmaceutical
compositions may contain suitable pharmaceutically acceptable
carriers comprising excipients and auxilliaries which facilitate
processing of the active compounds into preparations which can be
used pharmaceutically. Further details on techniques for
formulation and administration may be found in the latest edition
of Remington's Pharmaceutical Sciences (Ed. Maack Publishing Co,
Easton, Pa.).
[0196] Pharmaceutical compositions for oral administration can be
formulated using pharmaceutically acceptable carriers well known in
the art in dosages suitable for oral administration. Such carriers
enable the pharmaceutical compositions to be formulated as tablets,
pills, dragees, capsules, liquids, gels, syrups, slurries,
suspensions and the like, for ingestion by the patient.
[0197] Pharmaceutical preparations for oral use can be obtained
through combination of active compounds with solid excipient,
optionally grinding a resulting mixture, and processing the mixture
of granules, after adding suitable auxilliaries, if desired, to
obtain tablets or dragee cores. Suitable excipients are
carbohydrate or protein fillers such as sugars, including lactose,
sucrose, mannitol, or sorbitol; starch from corn, wheat, rice,
potatoe, or other plants; cellulose such as methyl, cellulose,
hydroxypropylmethylcellulose, or sodium carboxymethylcellulose; and
gums including arabic and tragacanth; and proteins such as gelatin
and collagen. If desired, disintegrating or solubilizing agents may
be added, such as the cross-linked polyvinyl pyrrolidone, agar,
alginic acid, or a salt thereof, such as sodium alginate.
[0198] Dragee cores are provided with suitable coatings such as
concentrated sugar solutions, which may also contain gum arabic,
talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol
and/or titanium dioxide, lacquer solutions, and suitable organic
solvents or solvent mixtures. Dyestuffs or pigments may be added to
the tablets or dragee coatings for product identification or to
characterize the quantity of active compound, ie. dosage.
[0199] Pharmaceutical preparations which can be used orally include
push-fit capsules made of gelatin, as well as soft, sealed capsules
made of gelatin and a coating such as glycerol or sorbitol.
Push-fit capsules can contain active ingredients mixed with a
filler or binders such as lactose or starches, lubricants such as
talc or magnesium stearate, and optionally, stabilizers. In soft
capsules, the active compounds may be dissolved or suspended in
suitable liquids, such as fatty oils, liquid paraffin, or liquid
polyethylene glycol with or without stabilizers.
[0200] Pharmaceutical formulations for parenteral administration
include aqueous solutions of active compounds. For injection, the
pharmaceutical compositions of the invention may be formulated in
aqueous solutions, preferably in physiologically compatible buffers
such as Hank's solution, Ringer's solution, or physiologically
buffered saline. Aqueous injection suspensions may contain
substances which increase viscosity of the suspension, such as
sodium carboxymethyl cellulose, sorbitol, or dextran. Additionally,
suspensions of the active compounds may be prepared as appropriate
oily injection suspensions. Suitable lipophilic solvents or
vehicles include fatty oils such as sesame oil, or synthetic fatty
acid esters, such as ethyl oleate or triglycerides, or liposomes.
Optionally, the suspension may also contain suitable stabilizers or
agents which increase the solubility of the compounds to allow for
the preparation of highly concentrated solutions.
[0201] For topical or nasal administration, penetrants appropriate
to the particular barrier to be permeated are used in the
formulation. Such penetrants are generally known in the art.
[0202] Kits
[0203] The invention further relates to pharmaceutical packs and
kits comprising one or more containers filled with one or more of
the ingredients of the aforementioned compositions of the
invention. Associated with such container(s) can be a notice in the
form prescribed by a governmental agency regulating the
manufacture, use or sale of pharmaceuticals or biological products,
reflecting approval by the agency of the manufacture, use or sale
of the product for human administration.
[0204] Manufacture and Storage
[0205] The pharmaceutical compositions of the present invention may
be manufactured in a manner that is known in the art, e.g., by
means of conventional mixing, dissolving, granulating,
dragee-making, levigating, emulsifying, encapsulating, entrapping
or lyophilizing processes.
[0206] The pharmaceutical composition may be provided as a salt and
can be formed with may acids, including by not limited to
hydrochloric, sulfuric, acetic, lactic, tartaric, malic, succinic,
etc. Salts tend to be more soluble in aqueous or other protonic
solvents that are the corresponding free base forms. In other
cases, the preferred preparation may be a lyophilized powder in 1
mM-50 mM histidine, 0.1%-2% sucrose, 2%-7% mannitol at a pH range
of 4.5 to 5.5 that is combined with buffer prior to use.
[0207] After pharmaceutical compositions comprising a compound of
the invention formulated in an acceptable carrier have been
prepared, they can be placed in an appropriate container and
labeled for treatment of an indicated condition. For administration
of HIAP3, such labeling would include amount, frequency and method
of administration.
[0208] Therapeutically Effective Dose
[0209] Pharmaceutical compositions suitable for use in the present
invention include compositions wherein the active ingredients are
contained in an effective amount to achieve the intended purpose,
i.e. treatment of a particular disease state characterized by
inappropriate apoptosis. The determination of an effective dose is
well within the capability of those skilled in the art.
[0210] For any compound, the therapeutically effective dose can be
estimated initially either in cell culture assays, e.g., neoplastic
cells, or in animal models, usually mice, rabbits, dogs, or pigs.
The animal model is also used to achieve a desirable concentration
range and route of administration. Such information can then be
used to determine useful doses and routes for administration in
humans.
[0211] A therapeutically effective dose refers to that amount of
protein or its antibodies, antagonists, or inhibitors which
ameliorate the symptoms or condition. Therapeutic efficacy and
toxicity of such compounds can be determined by standard
pharmaceutical procedures in cell cultures or experimental animals,
e.g., ED.sub.50 (the dose therapeutically effective in 50% of the
population) and LD.sub.50 (the dose lethal to 50% of the
population). The dose ratio between therapeutic and toxic effects
is the therapeutic index, and it can be expressed as the ratio,
ED.sub.50/LD.sub.50. Pharmaceutical compositions which exhibit
large therapeutic indices are preferred. The data obtained from
cell culture assays and animal studies is used in formulating a
range of dosage for human use. The dosage of such compounds lies
preferably within a range of circulating concentrations what
include the ED.sub.50 with little or no toxicity. The dosage varies
within this range depending upon the dosage form employed,
sensitivity of the patient, and the route of administration.
[0212] The exact dosage is chosen by the individual physician in
view of the patient to be treated. Dosage and administration are
adjusted to provide sufficient levels of the active moiety or to
maintain the desired effect. Additional factors which may be taken
into account include the severity of the disease state, eg, tumor
size and location; age, weight and gender of the patient; diet,
time and frequency of administration, drug combination(s), reaction
sensitivities, and tolerance/response to therapy. Long acting
pharmaceutical compositions might be administered every 3 to 4
days, every week, or once every two weeks depending on half-life
and clearance rate of the particular formulation.
[0213] Normal dosage amounts may vary from 0.1 to 100,000
micrograms, up to a total dose of about 1 g, depending upon the
route of administration. Guidance as to particular dosages and
methods of delivery is provided in the literature. See U.S. Pat.
Nos. 4,657,760; 5,206,344; or 5,225,212. Those skilled in the art
will employ different formulations for polynucleotides than for
proteins or their inhibitors. Similarly, delivery of
polynucleotides or polypeptides will be specific to particular
cells, conditions, locations, etc.
[0214] Gene Therapy
[0215] The hiap3 polynucleotides and HIAP3 polypeptides of the
present invention may be employed in accordance with the present
invention by expression of such polypeptides in vivo, in treatment
modalities often referred to as "gene therapy."
[0216] Thus, for example, cells from a patient may be engineered
with a polynucleotide, such as a DNA or RNA, encoding a polypeptide
ex vivo, and the engineered cells then can be provided to a patient
to be treated with the polypeptide. For example, cells may be
engineered ex vivo by the use of a retroviral plasmid vector
containing RNA encoding a polypeptide of the present invention.
Such methods are well-known in the art and their use in the present
invention will be apparent from the teachings herein.
[0217] Similarly, cells may be engineered in vivo for expression of
a polypeptide in vivo by procedures known in the art. For example,
a polynucleotide of the invention may be engineered for expression
in a replication defective retroviral vector, as discussed above.
The retroviral expression construct then may be isolated and
introduced into a packaging cell is transduced with a retroviral
plasmid vector containing RNA encoding a polypeptide of the present
invention such that the packaging cell now produces infectious
viral particles containing the gene of interest. These producer
cells may be administered to a patient for engineering cells in
vivo and expression of the polypeptide in vivo. These and other
methods for administering a polypeptide of the present invention by
such method should be apparent to those skilled in the art from the
teachings of the present invention.
[0218] Retroviruses from which the retroviral plasmid vectors
herein above mentioned may be derived include, but are not limited
to, Moloney Murine Leukemia Virus, spleen necrosis virus,
retroviruses such as Rous Sarcoma Virus, Harvey Sarcoma Virus,
avian leukosis virus, gibbon ape leukemia virus, human
immunodeficiency virus, adenovirus, Myeloproliferative Sarcoma
Virus, and mammary tumor virus. In one embodiment, the retroviral
plasmid vector is derived from Moloney Murine Leukemia Virus.
[0219] Such vectors will include one or more promoters for
expressing the polypeptide. Suitable promoters which may be
employed include, but are not limited to, the retroviral LTR; the
SV4O promoter; and the human cytomegalovirus (CMV) promoter (Miller
et al., Biotechniques 7:980-990, 1989), or any other promoter
(e.g., cellular promoters such as eukaryotic cellular promoters
including, but not limited to, the histone, RNA polymerase III,
and, .beta. actin promoters). Other viral promoters which may be
employed include, but are not limited to, adenovirus promoters,
thymidine kinase (TK) promoters, and B19 parvovirus promoters. The
selection of a suitable promoter will be apparent to those skilled
in the art from the teachings contained herein.
[0220] The polynucleotide sequence encoding the polypeptide of the
present invention will be placed under the control of a suitable
promoter. Suitable promoters which may be employed include, but are
not limited to, adenoviral promoters, such as the adenoviral major
late promoter; or heterologous promoters, such as the
cytomegalovirus CMV) promoter; the respiratory syncytial virus
(RSV) promoter; inducible promoters, such as the MMT promoter, the
metallothionein promoter; heat shock promoters; the albumin
promoter; the ApoAl promoter; human globin promoters; viral
thymidine kinase promoters such as the Herpes Simplex thymidine
kinase promoter; retroviral LTRs (including the modified retroviral
LTRs herein above described); the .beta.-actin promoter; and human
growth hormone promoters. The promoter also may be the native
promoter which controls the gene encoding the polypeptide.
[0221] The retroviral plasmid vector is employed to transduce
packaging cell lines to form producer cell lines. Examples of
packaging cells which may be transfected include, but are not
limited to, the PE5OI, PA317 Y-2, Y-AM, PAI2, T19-14X, VT-19-17-H2,
YCRE, YCRIP, GP+E-86, GP+envAml2, and DAN cell lines as described
in Miller, A., Human Gene Therapy 1: 5-14, 1990. The vector may be
transduced into the packaging cells through any means known in the
art. Such means include, but are not limited to, electroporation,
the use of liposomes, and CaPO.sub.4 precipitation. In one
alternative, the retroviral plasmid vector may be encapsulated into
a liposome, or coupled to a lipid, and then administered to a
host.
[0222] The producer cell line will generate infectious retroviral
vector particles, which include the polynucleotide sequence(s)
encoding the polypeptides. Such retroviral vector particles then
may be employed to transduce eukaryotic cells, either in vitro or
in vivo. The transduced eukaryotic cells will express the
polynucleotide(s) encoding the polypeptide. Eukaryotic cells which
may be transduced include, but are not limited to, embryonic stem
cells, embryonic carcinoma cells, as well as hematopoietic stem
cells, hepatocytes, fibroblasts, myoblasts, keratinocytes,
endothelial cells, and bronchial epithelial cells.
[0223] Therapeutic Use of Antisense Vectors and Ribozymes
[0224] Expression vectors derived from retroviruses, adenovirus,
herpes or vaccinia viruses, or from various bacterial plasmids, may
be also be used for construction and delivery of recombinant
vectors which will express anti-sense hiap3. See, for example, the
techniques described in Sambrook et al. (supra) and Ausubel et al.
(supra).
[0225] The polynucleotides comprising full length cDNA sequence
and/or its regulatory elements enable researchers to use hiap3
polynucleotides as an investigative tool in sense (Youssoufian and
Lodish, Mol. Cell. Biol. 13:98-104, 1993) or antisense (Eguchi, et
al., Annu. Rev. Biochem. 60:631-652, 1991) regulation of gene
function. Such technology is now well known in the art, and sense
or antisense oligomers, or larger fragments, can be designed from
various locations along the coding or control regions.
[0226] Genes encoding HIAP3 can be turned off by transfecting a
cell or tissue with expression vectors which express high levels of
a desired HIAP3 fragment. Such constructs can flood cells with
untranslatable sense or antisense sequences. Even in the absence of
integration into the DNA, such vectors may continue to transcribe
RNA molecules until all copies are disabled by endogenous
nucleases. Transient expression may last for a month or more with a
non-replicating vector and even longer if appropriate replication
elements are part of the vector system.
[0227] As mentioned above, modification of gene expression can be
obtained by designing antisense molecules, DNA or RNA, to control
regions of hiap3, ie, the promoters, enhancers, and introns.
Oligonucleotides derived from the transcription initiation site,
eg, between -10 and +10 regions of the leader sequence, are
preferred. The antisense molecules may also be designed to block
translation of mRNA by preventing the transcript from binding to
ribosomes. Similarly, inhibition can be achieved using "triple
helix" base-pairing methodology. Triple helix pairing compromises
the ability of the double helix to open sufficiently for the
binding of polymerases, transcription factors, or regulatory
molecules. Recent therapeutic advances using triplex DNA were
reviewed by Gee, J. E. et al. (In Huber and Car, Molecular and
Immunologic Approaches, Futura Publishing Co., Mt. Kisco, N.Y.,
1994).
[0228] Ribozymes are enzymatic RNA molecules capable of catalyzing
the specific cleavage of RNA (U.S. Pat. No. 4,987,071; WO
93/23057). The mechanism of ribozyme action involves
sequence-specific hybridization of the ribozyme molecule to
complementary target RNA, followed by endonucleolytic cleavage.
Within the scope of the invention are engineered hammerhead motif
ribozyme molecules that can specifically and efficiently catalyze
endonucleolytic cleavage of RNA encoding HIAP3.
[0229] Specific ribozyme cleavage sites within any potential RNA
target are initially identified by scanning the target molecule for
ribozyme cleavage sites which include the following sequences, GUA,
GUU and GUC. Once identified, short RNA sequences of between 15 and
20 ribonucleotides corresponding to the region of the target gene
containing the cleavage site may be evaluated for secondary
structural features which may render the oligonucleotide
inoperable. The suitability of candidate targets may also be
evaluated by testing accessibility to hybridization with
complementary oligonucleotides using ribonuclease protection assays
(Irie et al., Advance. Pharmacol. 40:207-257, 1997).
[0230] Antisense molecules and ribozymes of the invention may be
prepared by any method known in the art for the synthesis of RNA
molecules. These include techniques for chemically synthesizing
oligonucleotides such as solid phase phosphoramidite chemical
synthesis. Alternatively, RNA molecules may be generated by in
vitro and in vivo transcription or DNA sequences encoding HIAP3.
Such DNA sequences may be incorporated into a wide variety of
vectors which suitable RNA polymerase promoters such as T7 or SP6.
Alternatively, antisense cDNA constructs that synthesize antisense
RNA constitutively or inducibly can be introduced into cell lines,
cells or tissues.
[0231] RNA molecules may be modified to increase intracellular
stability and half-life. Possible modifications include, but are
not limited to, the addition of flanking sequences at the 5' and/or
3' ends of the molecules or the use of phosphorothioate or 2'
O-methyl rather than phosphodiesterase linkages within the backbone
of the molecule. Increased stability can also be achieved by the
inclusion of nontraditional bases such as inosine and queosine as
well as acetyl-, methyl-, thio- and similarly modified forms of
adenine, cytidine, guanine, thymine, and uridine which are not as
easily recognized by endogenous endonucleases.
[0232] Methods for introducing vectors into cells or tissues
include those methods discussed infra and which are equally
suitable for in vivo, in vitro and ex vivo therapy. For ex vivo
therapy, vectors are introduced into stem cells taken from the
patient and clonally propagated for autologous transplant back into
that same patient as presented in U.S. Pat. Nos. 5,399,493 and
5,437,994, disclosed herein by reference. Delivery by transfection
and by liposome are quite will known in the art.
EXAMPLES
[0233] The present invention is further described by the following
examples. The examples are provided solely to illustrate the
invention by reference to specific embodiments. These
exemplifications, while illustrating certain specific aspects of
the invention, do not portray the limitations or circumscribe the
scope of the disclosed invention.
[0234] All examples were carried out using standard techniques,
which are well known and routine to those of skill in the art,
except where otherwise described in detail. Routine molecular
biology techniques of the following examples can be carried out as
described in standard laboratory manuals, such as Sambrook et al.,
Molecular Cloning: A Laboratory Manual, 2nd Ed.; Cold Spring Harbor
Laboratory Press, Cold Spring Harbor, N.Y., 1989.
[0235] 1. Isolation of Human iap3 cDNA Clone
[0236] A 226 bp EST sequence (Incyte Clone #1419118) was identified
based on analysis of the Incyte EST database using the polypeptide
consensus sequence of the BIR domain of IAP. tblastn database (GCG
package) searches using the peptide sequence predicted from the EST
sequence revealed a significant degree of similarity between amino
acid residues 19 and 75 of this gene product and the partial BIR
domain of human inhibitor of apoptosis protein 1(HIAP1). There is a
42% to 47% identity between this 57 amino acid gene product and
partial BIR1, BIR2 and BIR3 sequences of HIAP1, suggesting that the
gene product is a homolog of HIAP1.
[0237] A. 5' and 3' RACE (5' and 3' Rapid Amplification of cDNA
End)
[0238] Oligonucleotide primers derived from the EST sequence was
used to clone 5'-end and 3'-end cDNA. Marathon-ready human fetal
kidney cDNA was purchased from Clontech Inc. (South San Francisco)
and used as template. RACE reactions were carried out according to
the manufacturer's instructions (Clontech Inc).
[0239] Oligonucleotide primers used in 5'RACE are:
1 5'CCTTCCTGGCTCCTGGGCACTTTCAGA3' 5'GCCCCCATAGCAGAAGAAGCACCTC3'
5'GACGCAACTCCTCAGAGCCCATG- CC3'
[0240] Oligonucleotide primers used in 3'RACE are
2 5'GGCATGGGCTCTGAGGAGTTG3' 5'CAAGGTGAGGTGCTTCTTCTGCTA3'
[0241] The PCR products were cloned into pCRII vector (Invitrogen).
A total of 12 clones were sequenced. 7 clones have sequences which
overlapped with the EST sequence.
[0242] B. DNA and Protein Sequence Analysis
[0243] The DNA sequences of the above clones and EST clone were
assembled using GCG assemble program. The resulting contiguous DNA
sequence was used to search databases. Two clones were identified
in Incyte database and one clone from public EST database. One
clone (2953985) was ordered from Incyte and sequenced. Sequencing
data showed that the cDNA sequence contains 1337 bp. This cDNA
sequence is designated hiap3. There is 169 bp in the 5'
untranslated region and 274 bp in the 3' untranslated region
flanking the coding region (FIGS. 1 and 3).
[0244] The coding region consists of 894 bp. An open reading frame
of 298 amino acid residues was identified. The initiation codon ATG
is located at polynucleotide position 170, which has an adequate
Kozak sequence with an upstream stop codon at polynucleotide
position 110 (FIGS. 1 and 3).
[0245] Analysis of the predicted protein sequence (FIG. 2) reveals
a single BIR domain from amino acid position 88 to 155 (FIG. 4).
There is approximately 50% identity of the BIR domain between HIAP3
and the third BIR domain of either human or mouse IAP1 or IAP2.
Analysis using the Genetic Computer Group PileUp algorithm
indicates very high homology with other members of the IAP family
(FIGS. 4 & 5). HIAP3 has the BIR signature residues including
three cysteines and a histidine. A RING domain containing a peptide
sequence from aa position 240 to 289 was also identified, which has
the signature cysteine, leucine and histidine residues (FIG. 4).
There is 84% identity of the RING domain between HIAP3 and human
HIAP-1. A PileUp analysis shows very high homology within the
members of the family (FIG. 5)
[0246] 2. Northern Blot Analysis
[0247] A 414 bp Ava1 fragment, isolated from a 5'RACE clone (#1-1L2
generated using primer 5'GCCCCCATAGCAGAAGAAGCACCTC3') was used to
probe Northern blots. This fragment was labeled with .sup.32P-dCTP
using high prime labeling kit purchased from Boehringer Mannheim.
Human Multiple Tissue Northern Blot I and II, Human Fetal Tissue
Blot, Human Immune System Tissue Blot (Clontech) and Human Multiple
Tumor Tissue Blot (Biochain) were probed at high stringency
following the supplier's instructions (Clontech). The results
indicates that hiap3 is expressed mainly in placenta, lymph node,
fetal kidney and kidney tumor. These experiments were confirmed
using a second probe, a 387 bp Narl-Kpnl fragment isolated from a
second 5'RACE clone (#9-9-1, generated using primer
5'CCTTCCTGGCTCCTGGGCACTTTCAGA3').
[0248] Analysis of the Incyte database using the BLAST algorithm
from the GCG package with the full length cDNA sequence of hiap3
generated a total of 9 EST clones (including the original EST
clone) in the Incyte database. These clones are 2953985, 1419118,
1418580, 3842242, 4589046, 1529102, 3673370, 1520835 and EST92646.
Three clones are from fetal kidney library, one clone from mast
cell library, one clone from dendritic cell library, one clone from
mononuclear cell derived from umbilical vein, one clone from
placenta, one clone from bladder tumor, and one clone from skin
tumor tissue. The distribution of these EST clones is similar to
that found in the Northern blot analysis and suggests that HIAP3
may play a role in development, hematopoietic/immune system and
tumor formation.
[0249] All publications and patents mentioned in the above
specification are herein incorporated by reference. While the
present invention has been described with reference to the specific
embodiments thereof, it should be understood by those skilled in
the art that various changes may be made and equivalents may be
substituted without departing from the true spirit and scope of the
invention. In addition, many modifications may be made to adapt a
particular situation, material, composition of matter, process,
process step or steps, to the objective, spirit and scope of the
present invention. All such modifications are intended to be within
the scope of the claims appended hereto.
Sequence CWU 1
1
7 1 1337 DNA Homo sapiens CDS (170)..(1066) 1 gggatactcc cctcccaggg
tgtctggtgg caggcctgtg cctatccctg ctgtccccag 60 ggtgggcccc
gggggtcagg agctccagaa gggccagctg ggcatattct gagattggcc 120
atcagccccc atttctgctg caaacctggt cagagccagt gttccctcc atg gga cct
178 Met Gly Pro 1 aaa gac agt gcc aag tgc ctg cac cgt gga cca cag
ccg agc cac tgg 226 Lys Asp Ser Ala Lys Cys Leu His Arg Gly Pro Gln
Pro Ser His Trp 5 10 15 gca gcc ggt gat ggt ccc acg cag gag cgc tgt
gga ccc cgc tct ctg 274 Ala Ala Gly Asp Gly Pro Thr Gln Glu Arg Cys
Gly Pro Arg Ser Leu 20 25 30 35 ggc agc cct gtc cta ggc ctg gac acc
tgc aga gcc tgg gac cac gtg 322 Gly Ser Pro Val Leu Gly Leu Asp Thr
Cys Arg Ala Trp Asp His Val 40 45 50 gat ggg cag atc ctg ggc cag
ctg cgg ccc ctg aca gag gag gaa gag 370 Asp Gly Gln Ile Leu Gly Gln
Leu Arg Pro Leu Thr Glu Glu Glu Glu 55 60 65 gag gag ggc gcc ggg
gcc acc ttg tcc agg ggg cct gcc ttc ccc ggc 418 Glu Glu Gly Ala Gly
Ala Thr Leu Ser Arg Gly Pro Ala Phe Pro Gly 70 75 80 atg ggc tct
gag gag ttg cgt ctg gcc tcc ttc tat gac tgg ccg ctg 466 Met Gly Ser
Glu Glu Leu Arg Leu Ala Ser Phe Tyr Asp Trp Pro Leu 85 90 95 act
gct gag gtg cca ccc gag ctg ctg gct gct gcc ggc ttc ttc cac 514 Thr
Ala Glu Val Pro Pro Glu Leu Leu Ala Ala Ala Gly Phe Phe His 100 105
110 115 aca ggc cat cag gac aag gtg agg tgc ttc ttc tgc tat ggg ggc
ctg 562 Thr Gly His Gln Asp Lys Val Arg Cys Phe Phe Cys Tyr Gly Gly
Leu 120 125 130 cag agc tgg aag cgc ggg gac gac ccc tgg acg gag cat
gcc aag tgg 610 Gln Ser Trp Lys Arg Gly Asp Asp Pro Trp Thr Glu His
Ala Lys Trp 135 140 145 ttc ccc agc tgt cag ttc ctg ctc cgg tca aaa
gga aga gac ttt gtc 658 Phe Pro Ser Cys Gln Phe Leu Leu Arg Ser Lys
Gly Arg Asp Phe Val 150 155 160 cac agt gtg cag gag act cac tcc cag
ctg ctg ggc tct tgg gac ccg 706 His Ser Val Gln Glu Thr His Ser Gln
Leu Leu Gly Ser Trp Asp Pro 165 170 175 tgg gaa gaa ccg gaa gac gca
gcc cct gtg gcc ccc tcc gtc cct gcc 754 Trp Glu Glu Pro Glu Asp Ala
Ala Pro Val Ala Pro Ser Val Pro Ala 180 185 190 195 tct ggg tac cct
gag ctg ccc aca ccc agg aga gag gtc cag tct gaa 802 Ser Gly Tyr Pro
Glu Leu Pro Thr Pro Arg Arg Glu Val Gln Ser Glu 200 205 210 agt gcc
cag gag cca gga ggg gtc agt cca gcc gag gcc cag agg gcg 850 Ser Ala
Gln Glu Pro Gly Gly Val Ser Pro Ala Glu Ala Gln Arg Ala 215 220 225
tgg tgg gtt ctt gag ccc cca gga gcc agg gat gtg gag gcg cag ctg 898
Trp Trp Val Leu Glu Pro Pro Gly Ala Arg Asp Val Glu Ala Gln Leu 230
235 240 cgg cgg ctg cag gag gag agg acg tgc aag gtg tgc ctg gac cgc
gcc 946 Arg Arg Leu Gln Glu Glu Arg Thr Cys Lys Val Cys Leu Asp Arg
Ala 245 250 255 gtg tcc atc gtc ttt gtg ccg tgc ggc cac ctg gtc tgt
gct gag tgt 994 Val Ser Ile Val Phe Val Pro Cys Gly His Leu Val Cys
Ala Glu Cys 260 265 270 275 gcc ccc ggc ctg cag ctg tgc ccc atc tgc
aga gcc ccc gtc cgc agc 1042 Ala Pro Gly Leu Gln Leu Cys Pro Ile
Cys Arg Ala Pro Val Arg Ser 280 285 290 cgc gtg cgc acc ttc ctg tcc
tag gccaggtgcc atggccggcc aggtgggctg 1096 Arg Val Arg Thr Phe Leu
Ser 295 cagagtgggc tccctgcccc tctctgcctg ttctggactg tgttctgggc
ctgctgagga 1156 tggcagagct ggtgtccatc cagcactgac cagccctgat
tccccgacca ccgcccaggg 1216 tggagaagga ggcccttgct tggcgtgggg
gatggcttaa ctgtacctgt ttggatgctt 1276 ctgaatagaa ataaagtggg
ttttccctgg aggtacccag caaaaaaaaa aaaaaaaaaa 1336 a 1337 2 298 PRT
Homo sapiens 2 Met Gly Pro Lys Asp Ser Ala Lys Cys Leu His Arg Gly
Pro Gln Pro 1 5 10 15 Ser His Trp Ala Ala Gly Asp Gly Pro Thr Gln
Glu Arg Cys Gly Pro 20 25 30 Arg Ser Leu Gly Ser Pro Val Leu Gly
Leu Asp Thr Cys Arg Ala Trp 35 40 45 Asp His Val Asp Gly Gln Ile
Leu Gly Gln Leu Arg Pro Leu Thr Glu 50 55 60 Glu Glu Glu Glu Glu
Gly Ala Gly Ala Thr Leu Ser Arg Gly Pro Ala 65 70 75 80 Phe Pro Gly
Met Gly Ser Glu Glu Leu Arg Leu Ala Ser Phe Tyr Asp 85 90 95 Trp
Pro Leu Thr Ala Glu Val Pro Pro Glu Leu Leu Ala Ala Ala Gly 100 105
110 Phe Phe His Thr Gly His Gln Asp Lys Val Arg Cys Phe Phe Cys Tyr
115 120 125 Gly Gly Leu Gln Ser Trp Lys Arg Gly Asp Asp Pro Trp Thr
Glu His 130 135 140 Ala Lys Trp Phe Pro Ser Cys Gln Phe Leu Leu Arg
Ser Lys Gly Arg 145 150 155 160 Asp Phe Val His Ser Val Gln Glu Thr
His Ser Gln Leu Leu Gly Ser 165 170 175 Trp Asp Pro Trp Glu Glu Pro
Glu Asp Ala Ala Pro Val Ala Pro Ser 180 185 190 Val Pro Ala Ser Gly
Tyr Pro Glu Leu Pro Thr Pro Arg Arg Glu Val 195 200 205 Gln Ser Glu
Ser Ala Gln Glu Pro Gly Gly Val Ser Pro Ala Glu Ala 210 215 220 Gln
Arg Ala Trp Trp Val Leu Glu Pro Pro Gly Ala Arg Asp Val Glu 225 230
235 240 Ala Gln Leu Arg Arg Leu Gln Glu Glu Arg Thr Cys Lys Val Cys
Leu 245 250 255 Asp Arg Ala Val Ser Ile Val Phe Val Pro Cys Gly His
Leu Val Cys 260 265 270 Ala Glu Cys Ala Pro Gly Leu Gln Leu Cys Pro
Ile Cys Arg Ala Pro 275 280 285 Val Arg Ser Arg Val Arg Thr Phe Leu
Ser 290 295 3 27 DNA artificial sequence race primer 1 3 ccttcctggc
tcctgggcac tttcaga 27 4 25 DNA artificial sequence race primer 2 4
gcccccatag cagaagaagc acctc 25 5 25 DNA artificial sequence race
primer 3 5 gacgcaactc ctcagagccc atgcc 25 6 21 DNA artificial
sequence race primer 4 6 ggcatgggct ctgaggagtt g 21 7 24 DNA
artificial sequence race primer 5 7 caaggtgagg tgcttcttct gcta
24
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