U.S. patent application number 09/861113 was filed with the patent office on 2001-11-01 for thin mineralized collagen membrane and method of making same.
Invention is credited to Liu, Sung-Tsuen.
Application Number | 20010037014 09/861113 |
Document ID | / |
Family ID | 23520593 |
Filed Date | 2001-11-01 |
United States Patent
Application |
20010037014 |
Kind Code |
A1 |
Liu, Sung-Tsuen |
November 1, 2001 |
Thin mineralized collagen membrane and method of making same
Abstract
A strong, thin flexible mineralized collagen membrane is
disclosed that is useful for such medical applications as a barrier
for guided tissue regeneration, periodontal defect repair, bone
grafts and skin wound repair. The mineralized collagen membrane
comprises a substantially homogeneous mineralized collagen
composite consisting essentially of about 30% to about 70% by
weight of a collagen component and about 30% to about 70% by weight
of a calcium phosphate minerals component precipitated from a
collagen slurry by a soluble calcium ion-containing solution and a
soluble phosphate ion-containing solution. The calcium phosphate
minerals component has a mole ratio of calcium to phosphate in the
range of about 1.0 to about 2.0. The thin mineralized collagen
membrane has a bulk density of the membrane of at least about 1.0
g/cm.sup.3 and has a thickness not greater than about 0.5 mm and
preferably not greater than about 0.3 mm. A corresponding method of
making the mineralized collagen membrane includes casting washed
mineralized collagen slurry as a thin, flat sheet onto a surface,
such as a sieve or filter paper, air drying the sheet and removing
it from the surface as the thin mineralized collagen membrane.
Inventors: |
Liu, Sung-Tsuen; (Laguna
Niguel, CA) |
Correspondence
Address: |
Howard R. Lambert
5245 Gatewood Lane
Anaheim
CA
92807
US
|
Family ID: |
23520593 |
Appl. No.: |
09/861113 |
Filed: |
May 19, 2001 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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09861113 |
May 19, 2001 |
|
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09385238 |
Aug 28, 1999 |
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Current U.S.
Class: |
530/356 |
Current CPC
Class: |
A61K 2300/00 20130101;
A61K 2300/00 20130101; C08L 89/06 20130101; A61L 31/127 20130101;
A61K 47/02 20130101; A61L 31/127 20130101; A61K 38/1875 20130101;
A61K 38/39 20130101; A61K 38/39 20130101; A61K 38/1875 20130101;
A61K 9/7007 20130101 |
Class at
Publication: |
530/356 ;
514/12 |
International
Class: |
A61K 038/17; C07K
017/00; C07K 016/00; C07K 014/00; C09H 001/00; C07K 001/00; A61K
038/00 |
Claims
What is claimed is:
1. A thin mineralized collagen membrane useful for medical
applications comprises a substantially homogeneous mineralized
collagen composite consisting essentially of about 30% to about 70%
by weight of a collagen component and about 30% to about 70% by
weight of a calcium phosphate minerals component precipitated from
a collagen slurry by a soluble calcium ion-containing solution and
a soluble phosphate ion-containing solution, said thin mineralized
collagen membrane having a thickness not greater than about 0.5
mm.
2. The thin mineralized collagen membrane as claimed in claim 1,
wherein said collagen component is selected to include natural
collagen, recombinant collagen or a mixture thereof.
3. The thin mineralized collagen membrane as claimed in claim 1,
wherein said calcium phosphate minerals are selected from the group
consisting of calcium phosphate, tri-calcium phosphate,
octa-calcium phosphate, calcium deficient apatite, amorphous
calcium phosphate, hydroxyapatite, substitute apatite, apatite-like
minerals, and mixture thereof.
4. The thin mineralized collagen membrane as claimed in claim 1,
wherein said calcium phosphate minerals component has a mole ratio
of calcium to phosphate in the range of about 1.0 to about 2.0.
5. The thin mineralized collagen membrane as claimed in claim 1,
wherein said calcium phosphate minerals component has a mole ratio
of calcium to phosphate of about 1.67.
6. The thin mineralized collagen membrane as claimed in claim 1,
wherein said mineralized collagen membrane is crosslinked.
7. The thin mineralized collagen membrane as claimed in claim 1,
wherein said mineralized collagen membrane is non-crosslinked.
8. The thin mineralized collagen membrane as claimed in claim 1,
wherein said mineralized collagen membrane includes a drug selected
from the group consisting of antibiotics, bone morphogenetic
proteins, other bone growth factors, skin growth factors,
antiscarring agents and mixtures thereof.
9. The thin mineralized collagen membrane as claimed in claim 1,
wherein said mineralized collagen membrane has a bulk density of at
least about 1.0 g/cm.sup.3.
10. The thin mineralized collagen membrane as claimed in claim 1,
wherein said mineralized collagen membrane has a thickness no
greater than about 0.3 mm.
11. A thin mineralized collagen membrane useful for medical
applications comprises a substantially homogeneous mineralized
collagen composite consisting essentially of about 30% to about 70%
by weight of a collagen component and about 30% to about 70% by
weight of a calcium phosphate minerals component selected from the
group consisting of calcium phosphate, tri-calcium phosphate,
octa-calcium phosphate, calcium deficient apatite, amorphous
calcium phosphate, hydroxyapatite, substitute apatite, apatite like
minerals, and mixture thereof, said calcium phosphate minerals
having a mole ratio of calcium to phosphate in the range of about
1.0 to about 2.0, said mineralized collagen membrane having a bulk
density of at least about 1.0 g/cm.sup.3 and having a thickness not
greater than about 0.5 mm.
12. The thin mineralized collagen membrane as claimed in claim 11,
wherein said calcium phosphate minerals component has a mole ratio
of calcium to phosphate of about 1.67.
13. The thin mineralized collagen membrane as claimed in claim 11,
wherein said mineralized collagen membrane includes a drug selected
from the group consisting of antibiotics, bone morphogenetic
proteins, other bone growth factors, skin growth factors,
antiscarring agents and mixtures thereof.
14. A method for producing a substantially homogeneous thin
mineralized collagen membrane comprising the steps of: a. forming a
collagen slurry; b. forming a soluble calcium ion-containing
solution; c. forming a soluble phosphate ion containing solution;
d. adding said soluble calcium ion-containing solution and said
soluble phosphate ion-containing solution to said collagen slurry
while stirring the collagen slurry and maintaining the pH of said
collagen slurry at least about 7, thereby inducing the
precipitation of calcium phosphate mineral in the collagen slurry
as mineralized collagen slurry; e. recovering said mineralized
collagen slurry by solid-liquid separation; f. washing the
recovered mineralized collagen slurry with pure water; g. casting
the washed mineralized collagen slurry into a thin sheet; and h.
drying said thin sheet to form a thin mineralized collagen membrane
having a thickness not greater than about 0.5 mm.
15. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, wherein the
step of adding said soluble calcium ion-containing solution and
said soluble phosphate ion-containing solution to said collagen
slurry includes adding said soluble calcium ion-containing solution
and said soluble phosphate ion-containing solution to said collagen
separately at different times.
16. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, wherein the
step of adding said soluble calcium ion-containing solution and
said soluble phosphate ion-containing solution to said collagen
slurry includes adding said soluble calcium ion-containing solution
and said soluble phosphate ion-containing solution to said collagen
substantially simultaneously.
17. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, wherein the
step of adding said soluble calcium ion-containing solution and
said soluble phosphate ion-containing solution to said collagen
slurry includes maintaining of the collagen slurry at a pH of at
least about 7.
18. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, wherein said
soluble calcium ion-containing solution and said soluble phosphate
ion-containing solution are formed so that the mineralized collagen
membrane comprises about 30% to about 70% calcium phosphate
minerals and about 30% to about 70% collagen.
19. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, wherein said
soluble calcium ion-containing solution and said soluble phosphate
ion containing solution are formed so that said calcium phosphate
minerals comprise calcium phosphate, tri-calcium phosphate,
octa-calcium phosphate, calcium deficient apatite, amorphous
calcium phosphate, hydroxyapatite, substitute apatite, apatite-like
minerals, or a mixture thereof.
20. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 14, including the
step of aging the collagen slurry for sufficiently long after the
step of adding said soluble calcium ion-containing solution and
said soluble phosphate ion-containing solution to said collagen to
ensure at least substantially complete precipitation of calcium
phosphate.
21. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 14, including the
step of adding a drug to the washed mineralized collagen
slurry.
22. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 21, wherein the
step of adding a drug to the washed mineralized collagen slurry
includes adding a drug selected from the group consisting of
antibiotics, bone morphogenetic proteins, other bone growth
factors, skin growth factors, antiscarring agents and mixtures
thereof.
23. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 21, wherein the
step of adding a drug to the washed mineralized collagen slurry
includes the steps of drying the mineralized collagen membrane,
soaking the dried mineralized collagen membrane in a solution of a
selected drug, and drying the drug soaked mineralized collagen
membrane to incorporate the drug into the mineralized collagen
membrane.
24. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 14, including the
step of adding a cross-linking reagent into the mineralized
collagen slurry before the slurry is formed into a thin sheet.
25. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 14, including the
step of soaking the dry mineralized collagen membrane in a collagen
cross-linking reagent.
26. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 25, including the
step, after the cross-linking process is completed, of soaking and
washing the membrane with pure water to remove any un-reacted
cross-linking reagent.
27. A method for producing a substantially homogeneous thin
mineralized collagen membrane comprising the steps of: a. forming a
collagen slurry; b. forming a soluble calcium ion-containing
solution; c. forming a soluble phosphate ion containing solution;
d. adding said soluble calcium ion-containing solution and said
soluble phosphate ion-containing solution to said collagen slurry
while stirring the collagen slurry and maintaining the pH of said
collagen slurry in the range of at least about 7, thereby inducing
the precipitation of calcium phosphate mineral in the collagen
slurry as mineralized collagen slurry, said soluble phosphate ion
containing solution being formed so that the mineralized collagen
membrane comprises about 10% to about 70% calcium phosphate
minerals and about 30% to about 70% collagen; e. aging the collagen
slurry for sufficiently long after the step of adding said soluble
calcium ion-containing solution and said soluble phosphate
ion-containing solution to said collagen to ensure at least
substantially complete precipitation of calcium phosphate; f.
recovering said mineralized collagen slurry by solid-liquid
separation; g. washing the recovered mineralized collagen slurry
with pure water; h. casting the washed mineralized collagen slurry
into a thin flat sheet on a filter media; and i. drying said thin
flat sheet and removing it from the filter media as a thin
mineralized collagen membrane having a thickness not greater than
about 0.3 mm.
28. The method for producing a substantially homogeneous thin
mineralized collagen membrane as claimed in claim 27, wherein said
soluble calcium ion-containing solution and said soluble phosphate
ion containing solution are formed so that said calcium phosphate
minerals comprise calcium phosphate, tri-calcium phosphate,
octa-calcium phosphate, calcium deficient apatite, amorphous
calcium phosphate, hydroxyapatite, substitute apatite, apatite-like
minerals, or a mixture thereof.
29. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 27, wherein said
step of adding said soluble calcium ion-containing solution and
said soluble phosphate ion-containing solution to said collagen
slurry while stirring the collagen slurry includes maintaining the
pH of said collagen slurry at not less than about 9.
30. The method for producing a substantially homogeneous
mineralized collagen membrane as claimed in claim 27, including the
step of adding a drug to the washed mineralized collagen slurry,
said drug being selected from the group consisting of antibiotics,
bone morphogenetic proteins, other bone growth factors, skin growth
factors, antiscarring agents and mixtures thereof.
31. A thin mineralized collagen membrane useful for medical
applications such guided tissue regeneration for periodontal defect
repairs, for covering bone defects, for bone grafts, for soft
tissue flaps over bone grafts, for skin wound healing, for skin
sealing, for bone substitutes, for a carrier for antibiotics, bone
and skin growth factors, and skin and bone repair materials, said
mineralized collagen membrane comprising a substantially
homogeneous mineralized collagen composite consisting essentially
of about 30% to about 70% by weight of a collagen component and
about 30% to about 70% by weight of a calcium phosphate minerals
component precipitated from a collagen slurry by a soluble calcium
ion-containing solution and a soluble phosphate ion-containing
solution, and having a bulk density of at least about 1.0
g/cm.sup.3 and having a thickness not greater than about 0.5
mm.
32. The thin mineralized collagen membrane as claimed in claim 31,
wherein said calcium phosphate minerals component has a mole ratio
of calcium to phosphate in the range of about 1.0 to about 2.0.
33. The thin mineralized collagen membrane as claimed in claim 31,
wherein said membrane has a thickness not greater than about 0.3
mm.
Description
[0001] This application is a Continuation-in Part (CIP) of
application Ser. No. 09/385,238, filed Aug. 28, 1999.
BACKGROUND OF THE INVENTION
[0002] 1. Field of the Invention
[0003] The present invention relates generally to the field of
mineralized collagen materials and methods of producing the same
and more particularly to thin mineralized collagen structures
useful for medical applications, such as guided tissue regeneration
(GTR) procedures, and methods for producing the thin mineralized
collagen structures.
[0004] 2. Background Discussion
[0005] By way of definition, collagen is an inert natural protein
found in human and animal skin, connective tissue, bones and teeth;
apatite is a calcium phosphate mineral and is the most important
mineral constituent of bones and teeth. With respect to the latter,
bone typically contains sixty to seventy-five percent by weight of
biological apatite and teeth typically contain more than
ninety-eight percent by weight of biological apatite.
[0006] Biological apatite has a crystal structure similar to that
of pure hydroxyapatite, but contains some substitute ions for
calcium, phosphate, and hydroxyl ions. Strictly speaking,
synthetically produced, precipitated hydroxyapatite is more similar
to biological apatite than are the hydroxyapatite ceramics.
[0007] It is already known that collagen can be mineralized by
calcium phosphate minerals by inducing the precipitation of calcium
phosphate in a collagen slurry. Several patents have disclosed the
methods of preparation mineralized collagen by this precipitation
of calcium phosphate.
[0008] As an example, my prior U.S. Pat. No. 5,320,844 discloses a
mineralized collagen composite material produced by quickly adding
a soluble calcium ion-containing solution and a phosphate
ion-containing solution into a collagen slurry. The patent further
discloses that the slurry is to be vigorously stirred while
maintaining it at a pH of 7 or higher to thereby induce the
mineralization of the collagen. The mineralized collagen is
disclosed as being recovered by solid-liquid separation and by then
being dried. However, my prior patent does not disclose a thin
mineralized collagen membrane or how such a membrane might be
made.
[0009] In addition, U.S. Pat. Nos. 5,455,231 and 5,231,169 and
foreign patent WO 93/12736 to Brent R. Constantz et al. describe
methods of mineralizing collagen by dispersing collagen in an
alkaline solution and subsequently mixing soluble calcium and
phosphate containing solutions to the collagen for over an hour
while maintaining the resulting collagen slurry at a pH of 10 or
higher.
[0010] The present inventor has observed that mineralized collagen
exhibits very different physical and mechanical properties than
those of pure collagen. For example, membranes made from collagen
only are weak in mechanical strength. Such collagen-only membranes
show swelling when soaked in water, are difficult and slippery to
handle, do not have the conductivity property needed for bone
growth and their resorption rate is difficult to control.
[0011] In contrast, mineralized collagen is considered by the
present inventor to provide better biocompatibility toward animal
and human tissue than does pure collagen. As hard tissue implant
material, mineralized collagen also has a conductivity effect that
enhances hard tissue (e.g., bone) growth. Moreover, mineralized
collagen has properties that are significantly different from
simple collagen-calcium phosphate mixtures, for example, as
disclosed in U.S. Pat. No. 5,425,770 to Karl A. Piez et al.
[0012] It is often necessary or desired to regenerate injured or
defective animal or human tissue, including hard tissue such as
bone. In order to assist such tissue generation, a so-called guided
tissue regeneration (GTR) barrier material is typically applied to
the tissue region to be regenerated.
[0013] So far as is known to the present inventor, mineralized
collagen has heretofore been prepared by freeze-drying to form a
sponge structure or is alternatively prepared in solid block or
granule form. However, none of these mineralized forms of collagen
can be used as membrane barriers for GTR procedures, and no strong,
mineralized collagen thin membranes that would be suitable for GTR
applications are believed by the present inventor to have been used
or disclosed.
[0014] For more than a decade, thin polytetrafluoroethylene (PTFE)
membranes have been used as barriers for GTR procedures in
repairing periodontal defects. However, such known PTFE membrane
barriers are non-resorbable. The use of such non-resorbable PTFE
membranes for GTR procedures requires a two step tissue
regeneration procedure. For example, in a first periodontal tissue
regeneration step, the non-resorbable barrier material is inserted
into or onto the periodontal defect. In a second step, after an
initial healing period of about four to six weeks, the barrier
material is surgically removed. This two step procedure associated
with the use of a non-resorbable GTR barrier material is relatively
costly, and the second, surgical-removal step increases the risk of
patient infection and of other undesirable side effects.
[0015] In an attempt to simplify and reduce the cost of GTR
procedures, thin, resorbable polymer membranes have recently been
developed and tested in GTR applications. So far as is known to the
present inventor, such resorbable polymers comprise either
polylactic acid blended with a citric acid ester or a copolymer
made of glycolide and lactide polymers. These polymers degrade
directly to acid during the resorption process.
[0016] Examples of such resorbable polymer membranes are the GUIDOR
product available from the John O. Butler Company and the CORE
RESOLUTE XT product available from W. L. Core Associates.
[0017] However, medical devices made from the above types of
resorbable polymer membranes have been associated with an
inflammatory response, which may be caused by the accumulation of
the acid of the degradation product.
[0018] More recently, membranes made from non-mineralized collagen
(for example, BIOMED material available from Sulzer Medica) have
been used in GTR applications for periodontal defect repair.
However, membranes made from non-mineralized collagen is normally
weak in strength and is therefore difficult to manipulate.
Furthermore, the resorption rate of such non-mineralized membranes
is difficult to match with the normal tissue-healing process.
[0019] It is, therefore, a principle objective of the present
invention to provide a strong, thin, flexible mineralized collagen
membrane that is biocompatable with human tissue, that is
resorbable in GTR applications and which has a controllable
resorption rate. It is also an objective to provide a method for
making such a mineralized collagen membrane.
[0020] Such a mineralized collagen membrane would eliminate the
above-mentioned second step of surgically removing the GTR barrier
and would thereby simplify and reduce the cost of the GTR
procedures for both soft tissue and hard tissue repair.
SUMMARY OF THE INVENTION
[0021] In accordance with the present invention a strong, thin
flexible mineralized collagen membrane useful for medical
applications comprises a substantially homogeneous mineralized
collagen composite. The composite consists essentially of between
about 30% and about 70% by weight of a collagen component and
between about 30% and about 70% by weight of a calcium phosphate
minerals component precipitated from a collagen slurry by a soluble
calcium ion-containing solution and a soluble phosphate
ion-containing solution. The collagen component may include natural
collagen and recombinant collagen. The thin mineralized collagen
membrane has a thickness that is preferably not greater than about
0.5 mm and a thickness that is more preferably not greater than
about 0.3 mm.
[0022] The mineralized collagen membrane useful for medical
applications preferably comprises a substantially homogeneous
mineralized collagen composite of between about 30% to about 70% by
weight of a collagen component and between about 30% and about 70%
by weight of calcium phosphate minerals. The calcium phosphate
minerals component is preferably selected from the group consisting
essentially of calcium phosphate, tri-calcium phosphate,
octa-calcium phosphate, calcium deficient apatite, amorphous
calcium phosphate, hydroxyapatite, substitute apatite, apatite-like
minerals, and mixture thereof. Preferably the calcium phosphate
minerals component has a mole ratio of calcium to phosphate in the
range of about 1.0 to about 2.0; more preferably, the mole ratio of
calcium to phosphate of about 1.67. Preferably, the mineralized
collagen membrane will have a bulk density of about 1.0 g/cm.sup.3
or higher.
[0023] A method of making or producing a substantially homogeneous
thin mineralized collagen membrane comprises the steps of forming a
collagen slurry, forming a soluble calcium ion-containing solution,
forming a soluble phosphate ion containing solution, and adding the
soluble calcium ion-containing solution and the soluble phosphate
ion-containing solution to the collagen slurry while stirring,
preferably vigorously stirring, the collagen slurry and maintaining
the pH of said collagen slurry at least about 7, thereby inducing
the precipitation of calcium phosphate mineral in the collagen
slurry as mineralized collagen slurry.
[0024] The method includes the further steps of recovering the
mineralized collagen slurry by solid-liquid separation, washing the
recovered mineralized collagen slurry with pure water, casting the
washed mineralized collagen slurry into a thin flat sheet, and
drying the thin flat sheet to form a thin mineralized collagen
membrane. The washed mineralized collagen slurry may be cast into a
thin sheet on a filter media, after which the thin sheet is dried,
preferably air dried, and is then removed from the filter media as
a thin mineralized collagen membrane. In either case, the thin
mineralized collagen membrane has a preferred thickness not greater
than about 0.5 mm and a more preferably thickness not greater than
about 0.3 mm.
[0025] The step of adding the soluble calcium ion-containing
solution and the soluble phosphate ion-containing solution to the
collagen slurry includes adding the soluble calcium ion-containing
solution and the soluble phosphate ion-containing solution to the
collagen either simultaneously or separately at different times,
and preferably includes maintaining the collagen slurry at a pH of
at least about 7.
[0026] Preferably, the steps of forming a soluble calcium
ion-containing solution and a soluble phosphate ion-containing
solution includes forming these solutions so that the mineralized
collagen membrane comprises between about 30% and about 70% by
weight of calcium phosphate minerals and between about 30% and
about 70% by weight of collagen. The soluble calcium ion-containing
solution and the soluble phosphate ion-containing solution are
formed so that the calcium phosphate minerals comprise calcium
phosphate, tri-calcium phosphate, octa-calcium phosphate, calcium
deficient apatite, amorphous calcium phosphate, hydroxyapatite,
substitute apatite, apatite-like minerals, or a mixture
thereof.
[0027] It is preferred that the method for producing a
substantially homogeneous mineralized collagen includes the step of
aging the collagen slurry sufficiently long after the step of
adding the soluble calcium ion-containing solution and the soluble
phosphate ion-containing solution to the collagen slurry to ensure
the near complete precipitation of calcium phosphate. Preferably,
the pH of the slurry is maintained at between about 7 and about 9
or higher, with the higher pH being preferred.
[0028] A drug or a combination of drugs may be incorporated into
the mineralized collagen membrane by adding the drug or drugs into
the purified mineralized collagen slurry before processing the
material into a thin membrane. Alternatively, the drug or drugs may
be incorporated by soaking the thin mineralized collagen membrane
into soluble drug solution and then drying the thin membrane. The
drug or drugs may include antibiotics, bone morphogenetic proteins,
other bone growth factors, skin growth factors, antiscarring agents
and/or combinations thereof.
[0029] The method may adding a cross-linking reagent into the
mineralized collagen slurry before the slurry is formed into a thin
sheet. Alternatively, the dry mineralized collagen membrane may be
soaked in a collagen cross-linking reagent, in which case, after
the cross-linking process is completed, the membrane is soaked and
washed with pure water to remove any un-reacted cross-linking
reagent.
[0030] The strong, thin flexible mineralized collagen membrane of
the present invention provides mechanical properties superior to
those of pure collagen membranes and has important medical
applications, such as use for a barrier for tissue regeneration
procedures, periodontal repair and wound healing.
DESCRIPTION OF THE PREFERRED EMBODIMENT
[0031] The present inventor has determined that the use of thin,
mineralized collagen membranes of the present invention is
particularly advantageous for use as a barrier for GTR procedure
because the membranes will be reabsorbed during the tissue healing
interval such that the above-mentioned, previous second step of
surgically removing the barrier is eliminated.
[0032] For preparation of the mineralized collagen membrane of the
present invention, any suitable collagen component, including
natural collagen or recombinant collagen, may be used. The natural
collagen sources may come from skin, tendon or bone of animals such
as bovine, porcine, equine, chicken or the like. The preferred
starting collagen materials is non-mineralized collagen. The
initial collagen material can be any solid form, solution or
slurry.
[0033] The initial step in the preparation of the membrane form
mineralized collagen is the preparation of a collagen slurry. If
solid collagen is used, it is preferably dispersed in an acidic or
alkaline solution to form a homogeneous gel-type slurry. The
concentration of the collagen slurry suitable for following
mineralization process is preferably between about 0.1 percent and
about 5.0 percent.
[0034] In general, either a soluble calcium ion-containing
component (for example, a calcium salt) or a soluble phosphate
ion-containing component (for example, a phosphate salt) is then
dissolved or otherwise directly combined into the collagen
slurry.
[0035] If a calcium ion-containing component is directly combined
into the collagen slurry, the second, phosphate ion-containing
component is preferably separately dissolved or otherwise combined
in a liquid medium, preferably water, to form a solution.
Alternatively, if a phosphate ion-containing component is directly
combined into the collagen slurry, the second, calcium
ion-containing component is preferably separately dissolved or
otherwise combined in a liquid medium, preferably water, to form a
solution. In either such case, the second (phosphate ion-containing
or calcium ion-containing) component is preferably quickly added
(for example, by pouring) into the collagen slurry.
[0036] Alternately, two separate solutions, one with a soluble
calcium ion-containing component and the other with a phosphate
ion-containing component, can be prepared, and the two solutions
are preferably quickly and simultaneously added (poured) into the
collagen slurry, or the two solutions may be added to the collagen
slurry slowly. Preferably, but not necessarily, stoichiometric
amounts of calcium and phosphate ions are added to the collagen
slurry.
[0037] In either case, during the combination step, the collagen
slurry is vigorously mixed or stirred to ensure the formation of
homogeneous slurry reaction product. Although, the rapidity of
adding the calcium ion-containing component or phosphate
ion-containing component, or both, to the collagen slurry is not
critical the addition is preferably quickly performed to assure a
homogeneous reaction product.
[0038] After the complete addition of the calcium ion-containing
and phosphate ion-containing components to the collagen slurry, the
slurry is either continuously stirred or allowed to stand
un-stirred until the calcium phosphate reaction product has
completely precipitated.
[0039] During the preparation procedure, the temperature of the
mixture is preferably maintained below about 40.degree. C.
Moreover, during the precipitation of calcium phosphate the
collagen slurry is preferably maintained at a pH value of at least
about 7.0 and more preferably at a pH value of at least about 9.
This pH control can be achieved by adding enough alkaline solution,
such as sodium hydroxide, potassium hydroxide or ammonium
hydroxide, to either the collagen slurry or the phosphate
ion-containing solution before its combining with the slurry.
[0040] A calcium phosphate saturated solution at a pH value near 8
or higher will normally induce the precipitation of hydroxyapatite,
substitute apatite or calcium apatite-like calcium phosphate
minerals.
[0041] Other components may also be incorporated into the calcium
phosphate mineral. For example, if carbonate apatite or fluoride
apatite is to be incorporated into the mineralized collagen
product, a soluble carbonate or soluble fluoride salt can be added
into the phosphate ion-containing solution before its addition to
the collagen slurry.
[0042] After the calcium phosphate mineral is completely
precipitated, the resulting mineralized collagen slurry is
separated and purified, for example, by being filtered and/or
centrifuged and/or washed several times until the material is free
of other soluble components, such as entrapped soluble
impurities.
[0043] The purified mineralized collagen is then reslurried with
sufficient water to form a homogeneous slurry. This homogeneous
mineralized collagen slurry is then filtered or cast into a thin
flat sheet and air dried to form a thin mineralized collagen
membrane. In this regard, the preferred procedure is to de-water
the diluted mineralized collagen slurry by sieving the slurry using
a flat-type of mesh sieve with mesh number of about 15 or higher.
The thin wet slurry sheet that remains on the sieve is then
dried--preferably, air dried--to form a thin mineralized collagen
membrane that is strong and flexible, and still retains its flat
shape and original size after drying and removal from the
sieve.
[0044] Alternatively, the mineralized collagen slurry may be cast
as a thin sheet onto any appropriate clean surface. The thin wet
slurry sheet on the surface is then dried--preferably, air
dried--to form the thin mineralized collagen membrane.
[0045] Preferably the dry thin mineralized collagen membrane formed
as described a has a thickness no greater than about 0.5 mm and
still more preferably has a thickness no greater than about 0.3
mm.
[0046] The calcium phosphate mineral deposited in the collagen
slurry at a slurry pH value near neutral or up to about 8 is most
likely calcium phosphate, tri-calcium phosphate, octa-calcium
phosphate (OCP), amorphous calcium phosphate (ACP), hydroxyapatite,
calcium deficient apatite (CDA), substitute apatite (such as
carbonate apatite and fluro-apatite), apatite-like minerals, or
mixtures thereof.
[0047] At a slurry pH of about 8 or higher, the most probable
precipitation product is hydroxyapatite or calcium apatite-like
minerals. In order to induce the precipitation of a calcium
apatite-like material in the collagen slurry, the preferred mole
ratio of calcium to phosphate is about 1 to about 2, and is more
preferably about 1.67. However, other mole ratios can also be
used.
[0048] The calcium phosphate mineralized collagen membrane produced
in the manner set forth above may be either crosslinked or
non-crosslinked and may have widely varying compositions and still
be useful for such medical applications as a barrier for GTR
applications. The present inventor has however unexpectedly
determined that such mineralized collagen membranes optimally
comprise about 30 percent to about 70 percent by weight of collagen
and about 30 percent to about 70 percent by weight of calcium
phosphate containing minerals.
[0049] The calcium and phosphate containing minerals included in
the mineralized collagen membrane of the present invention are
preferably selected from calcium phosphates, calcium apatites
(including calcium-deficient apatite, hydroxyapatite, calcium
fluro-apatite, calcium carbonate apatite, and the like apatite-type
minerals) and mixtures thereof.
[0050] Mineralized collagen membranes including calcium
fluro-apatite can be produced by including fluoride (fluoride ion)
in the phosphate containing solution or collagen slurry before the
mineralization reaction take place.
[0051] The present mineralized collagen membrane may include one or
more additional components useful to provide one or more desired
properties to the composite membrane. Metals, such as alkali
metals, other alkaline earth metals and the like, may replace at
least a portion, for example, a minor portion, of the calcium. At
least a portion, for example, a minor portion, of the phosphate
and/or hydroxyl content of the calcium ion-containing and phosphate
ion-containing minerals may be replaced by a halogen, such as a
chloride or a fluoride, a carbonate and the like. The precipitated
calcium ion-containing and phosphate ion-containing minerals can
have a chemical composition which resembles or even simulates that
of biological apatite.
[0052] As is evident from the foregoing description, different
components may be added to collagen slurry, soluble calcium
ion-containing solution and/or phosphate ion-containing solution
used to make the present composite membrane to adjust the chemical
composition of the resulting mineralized collagen membrane as may
be desired.
[0053] Any suitable, preferably soluble and more preferably water
soluble, calcium ion-containing component may be employed in the
present methods of making mineralized collagen composite membranes,
provided that such calcium ion-containing components function as
described herein and result in a useful calcium phosphate
mineralized collagen membrane. Calcium ion-containing salts and
mixtures thereof are particularly useful. Example of calcium
ion-containing salts includes calcium acetate, calcium chloride,
calcium nitrate, calcium carboxylate, other soluble calcium organic
salts and mixtures thereof.
[0054] Any suitable, preferably soluble and more preferably water
soluble, phosphate ion-containing component may be employed in the
present methods for making calcium phosphate mineralized collagen
membranes, provide that such phosphate ion-containing components
function as described herein and result in a useful calcium
phosphate mineralized collagen membrane. Phosphate ion-containing
salts and mixtures thereof are particularly useful. Examples of
phosphate ion-containing salts include alkali metal phosphate
salts, ammonium phosphate salts and mixture thereof.
[0055] Although any suitable liquid medium may be employed (for
example, for the slurry, and/or the described solution or the
solutions), the use of an aqueous liquid media is much
preferred.
[0056] Various different materials may be employed to maintain or
adjust the pH of the collagen slurry, the preparation of a calcium
ion-containing solution and a phosphate ion-containing solution.
Example of useful pH adjusting agents includes alkaline metal
hydroxide, ammonium hydroxide and mixtures thereof.
[0057] Furthermore, the mineralized collagen membranes of the
present invention can also incorporate drugs such as antibiotics,
bone morphogenetic proteins, other bone growth factors, skin growth
factors or anti-scarring agents such as transforming growth
factor-Beta. In such case, the drugs will be preferably added to
the mineralized collagen slurry before its processing into the
mineralized collagen membrane form.
[0058] In order to enhance the mechanical strength of the
mineralized collagen membranes, a collagen cross-linking reagent
can be added into the mineralized collagen slurry after the
precipitation and before the purification steps described above. As
an alternative, the dry mineralized collagen membrane may be soaked
in the collagen cross-linking reagent. After the cross-linking
process is completed, the membrane is then soaked and washed with
pure water to remove any un-reacted cross-linking reagent.
[0059] In addition or alternatively, drugs, such as antibiotics,
bone morphogenetic proteins, other bone growth factors, skin growth
factors, antiscarring agents and mixtures thereof may be added to
the purified mineralized collagen slurry before processing it into
membrane form. Alternately, the drug may be incorporated into the
mineralized collagen membrane by soaking the membrane in a drug
solution and drying the resulting drug-containing mineralized
collagen membrane.
[0060] The present mineralized collagen membrane contains calcium
phosphate minerals which are incorporated into the structure of
collagen fibrils, the membrane being substantially homogeneous,
preferably with little or no phase separation visually
apparent.
[0061] Moreover, the present mineralized collagen membrane has
substantial compositional flexibility and the weight ratio of
precipitated calcium phosphate minerals to collagen component can
be varied over a relatively wide range. By so doing, the mechanical
strength and/or bioresoption rate of the present mineralized
collagen membrane can be varied over a relatively wide range.
[0062] It is apparent that the present membrane is quite different
from a pure collagen membrane. Pure collagen membranes without
cross-linking are very weak when soaked in water and show high
degrees of swelling. Furthermore, pure collagen membranes are
difficult to handle and the bioresorption rate is difficult to
control.
[0063] Surprisingly, the mineralized collagen membrane of the
present invention has strong mechanical strength even without the
described collagen cross-linking. When the present membrane is
soaked in water, it does not visibly swell and retains its
integrity even after months of aging in an aqueous environment.
[0064] Further, the bioresorption rate of my new mineralized
collagen membrane can be controlled by changing the content of
calcium phosphate minerals. Still further, this mineralized
collagen membrane has both excellent biocompatibility and
conductivity effects for bone growth.
[0065] My new mineralized collagen membrane is considered to have
many medical applications because of its flexibility, strong
mechanical strength, easy manipulation character, excellent
biocompability and controllable bioresorption rate. Such medical
applications include membrane barriers for GTR applications for
repairing periodontal defects, membranes for covering bone defect
surgery and for bone substitutes, skin wound repair and healing,
skin sealing, and as a carrier for antibiotic, bone growth factors,
skin growth factors, and so forth.
EXAMPLE 1
[0066] 1.0 gram (g) of solid fibril collagen (type 1 collagen) is
added into 200 milliliters (ml) of water. 0.3 ml glacial acetic
acid is then added to this aqueous mixture which is then stirred
(mixed) in a blender. At this stage the collagen is in the form of
a homogeneous gel slurry.
[0067] 2.2 g of CaCl.sub.2.2H.sub.2O is dissolved in 30 ml of pure
water to form a calcium chloride solution, and 2.0 g of
(NH.sub.4)2HPO.sub.4 is dissolved in a 30 ml of pure water to form
an ammonium phosphate solution.
[0068] The pHs of the collagen slurry, the calcium chloride
solution and the ammonium phosphate solution are separately
adjusted to a value of about 9 by adding ammonium hydroxide before
mixing together in the blender.
[0069] The collagen slurry is kept in the blender and stirred
vigorously. Both the calcium chloride solution and the ammonium
phosphate solution are poured simultaneously into the collagen
slurry. The stirring is continued for several more minutes and then
kept unstirred for about 1 hour. The resulting mineralized collagen
slurry is then filtered with a 18.5 cm diameter filter paper, and
is washed several times with pure water.
[0070] The filtered mineralized collagen is reslurried with water
and is then homogeneously spread on the filter paper and is allowed
to air dry to form a thin mineralized collagen membrane sheet.
After air drying, the mineralized collagen membrane sheet is then
separated from the filtration paper. The thin mineralized collagen
membrane thus formed has 40% by weight of collagen and 60% by
weight of calcium phosphate, and is strong and flexible.
[0071] The dried mineralized collagen membrane is visually examined
and does not show separate phases of collagen and calcium
phosphate.
EXAMPLE 2
[0072] 1 g of collagen sheet is dispersed in 200 ml of pure water
in a blender, and 0.6 g of solid NaOH is added and the mixture is
stirred for several minutes to form a collagen slurry.
[0073] 2.2 g CaCl.sub.2.2H.sub.2O is dissolved in 50 ml of pure
water in a 100 ml beaker.
[0074] 2.5 g (NH.sub.4).sub.2HPO.sub.4 is separately dissolved in
50 ml of pure water in another 100 ml beaker.
[0075] While the collagen slurry is stirred vigorously, both
aqueous solutions of calcium chloride and ammonium phosphate are
simultaneously poured quickly into the collagen slurry. The
resulting mixture (slurry) is then continued to be stirred in the
blender for several more minutes.
[0076] After allowing the slurry to stand still for about one
half-hour, the slurry is then cast into a 20 cm diameter, 20 mesh
metal sieve and is washed with pure water several times. The slurry
is then spread homogeneously on the top of the sieve and is
air-dried to form a thin mineralized collagen membrane. After air
drying, the mineralized collagen membrane is strong and flexible
and weighs 2.45 g, with a composition that is 40% by weight of
collagen and 60% by weight of calcium phosphate. The thin
mineralized collagen membrane has a surface area of 314 cm.sup.2
and has unit weight of 0.0073 g/cm.sup.2 and thickness of 0.0625
mm. The apparent bulk density of the membrane is higher than about
1.1 g/cm.sup.3.
EXAMPLE 3
[0077] This EXAMPLE 3 is similar to EXAMPLE 2 set forth above,
except that 2 g of collagen sheet, 4.4 g of CaCl.sub.2.2H.sub.2O
and 5.5 g (NH.sub.4).sub.2HPO.sub.4 are used. The thin dried
mineralized collagen membrane has the same surface area as in
EXAMPLE 2, but has a unit weight of 0.015 g/cm.sup.2 and thickness
of 0.125 mm. No separate phases of collagen and calcium phosphate
are observed. After being soaked in water for several weeks, this
particular mineralized collagen membrane still retains its
integrity and shows no detectable swelling.
[0078] As above stated, the present inventor has determined that
thin mineralized collagen membranes having thicknesses greater than
those disclosed in the foregoing specific EXAMPLES are useful for
medical applications.
[0079] Although there has been described above thin mineralized
collagen membranes and methods for making the membranes in
accordance with the present invention for purposes of illustrating
the manner in which the present invention maybe used to advantage,
it is to be understood that the invention is not limited thereto.
Consequently, any and all variations and/or equivalent methods
which may occur to those skilled in the applicable art are to be
considered to be within the scope and spirit of the invention as
set forth in the claims which are appended hereto as part of this
application.
* * * * *