U.S. patent application number 09/817887 was filed with the patent office on 2001-10-04 for comb copolymers for regulating cell-surface interactions.
Invention is credited to Banerjee, Pallab, Griffith, Linda G., Irvine, Darrell J., Johnson, Terry D., Mayes, Anne M..
Application Number | 20010027237 09/817887 |
Document ID | / |
Family ID | 22165144 |
Filed Date | 2001-10-04 |
United States Patent
Application |
20010027237 |
Kind Code |
A1 |
Mayes, Anne M. ; et
al. |
October 4, 2001 |
Comb copolymers for regulating cell-surface interactions
Abstract
Synthetic comb copolymers which elicit controlled cellular
response, methods of applying these polymers to various surfaces,
and methods of using the polymers for modifying biomaterial
surfaces, in tissue engineering applications and as drug delivery
devices are provided. The comb copolymers are comprised of
hydrophobic polymer backbones and hydrophilic, non-cell binding
side chains which can be end-capped with cell-signaling ligands
that guide cellular response. By mixing non-cell binding combs with
ligand-bearing combs, the surface concentration and spatial
distribution of one or more types of ligands, including adhesion
peptides and growth factors, can be tuned on a surface to achieve
desired cellular response. In one embodiment, the combs are used as
stabilizing agents for dispersion polymerization of latexes. The
comb-stabilized latexes can be applied to substrates by standard
coating operations to create a bioregulating surface, or used as
drug delivery agents. In another embodiment, the combs can be
blended in small quantities to a hydrophobic matrix polymer and
processed to affect the surface segregation of the comb. The comb
copolymers are formed in one embodiment by providing a
biodegradable polyester backbone that includes reactive groups, and
reacting the reactive groups in the backbone with reactive chain
ends on a low molecular weight hydrophilic polymer. In another
embodiment, non-biodegradable comb copolymers are formed by free
radical synthesis of a hydrophobic monomer and a hydrophilic
macromonomer. In all of the above embodiments, a portion of the
hydrophilic polymer side chains can be covalently coupled to
cell-signaling ligands such as adhesion peptides or growth factors
to control cellular response.
Inventors: |
Mayes, Anne M.; (Waltham,
MA) ; Griffith, Linda G.; (Cambridge, MA) ;
Irvine, Darrell J.; (Cambridge, MA) ; Banerjee,
Pallab; (Boston, MA) ; Johnson, Terry D.;
(Allston, MA) |
Correspondence
Address: |
PATREA L. PABST
HOLLAND & KNIGHT LLP
SUITE 2000, ONE ATLANTIC CENTER
1201 WEST PEACHTREE STREET, N.E.
ATLANTA
GA
30309-3400
US
|
Family ID: |
22165144 |
Appl. No.: |
09/817887 |
Filed: |
March 26, 2001 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
09817887 |
Mar 26, 2001 |
|
|
|
09634095 |
Aug 8, 2000 |
|
|
|
6207749 |
|
|
|
|
09634095 |
Aug 8, 2000 |
|
|
|
09290140 |
Apr 13, 1999 |
|
|
|
6150459 |
|
|
|
|
60081596 |
Apr 13, 1998 |
|
|
|
Current U.S.
Class: |
525/326.1 ;
424/422; 424/78.08; 424/78.17; 424/78.18; 525/418; 525/474 |
Current CPC
Class: |
C08G 81/00 20130101;
C08J 5/24 20130101; A61K 47/56 20170801; A61K 47/641 20170801; C08G
81/024 20130101; A61K 47/50 20170801 |
Class at
Publication: |
525/326.1 ;
525/418; 525/474; 424/78.17; 424/78.18; 424/78.08; 424/422 |
International
Class: |
A61K 047/48; A61K
031/74; C08F 008/00; C08F 283/00; C08F 265/00; C08F 283/12; C08G
077/38; C08G 063/91 |
Goverment Interests
[0002] The United States government has certain rights in this
invention by virtue of National Science Foundation grant No.
DMR-9400334, OSP Project No. 6227 to Anne M. Mayes, and National
Science Foundation Grant No. BES 9632714 to L. G. Griffith.
Claims
We claim:
1. A cell-regulating, comb-type copolymer comprising: a) a
hydrophobic polymer backbone; b) non-cell binding hydrophilic
polymeric side chains grafted to the polymer backbone, wherein the
side chains have a molecular weight between 200 and 2000 Daltons;
wherein between zero and 100% of the non-cell binding, hydrophilic
side chains are end-capped with cell-binding or cell-signaling
ligands to form short cell-binding copolymer side chains and
wherein the side chains comprise less than 60% of the total
copolymer weight.
2. The comb copolymer of claim 1 having a total molecular weight of
greater than 10,000 Daltons.
3. The comb copolymer of claim 1, wherein the backbone is
biodegradable.
4. The copolymer of claim 1, wherein the backbone is
non-biodegradable.
5. The copolymer of claim 1 wherein the side chains are less than
500 Daltons and constitute less than 60% of the total copolymer
weight.
6. The copolymer of claim 1 wherein the mole percentage of backbone
segments attached to hydrophilic side chains is between 2 and
30%.
7. The copolymer of claim 1 wherein the percent of hydrophilic side
chains which include functional groups capable of being covalently
or ionically attached to a cell-binding or cell-signaling ligand is
between 1 and 20%.
8. The copolymer of claim 1, wherein the non-cell binding side
chains are selected from the group consisting of polyethylene
glycol, polyethylene oxide, polyacrylic acid and dextran.
9. The copolymer of claim 1, wherein the ligands are selected from
the group consisting of adhesion peptides, cell-signaling peptides
and growth factors.
10. The copolymer of claim 1 in a mixture further comprising
non-cell-binding comb copolymers whose side chains are not
end-capped with cell-binding or cell-signaling ligands.
11. The comb copolymer mixture of claim 10 wherein less than 20% of
the comb copolymers comprise side chains that are end-capped with
cell-binding or cell-signaling ligands.
12. A tissue engineering matrix, cell culture matrix, biomedical
device, or implant formed of or coated with the cell-regulating,
comb-type copolymer comprising: a) a hydrophobic polymer backbone;
b) non-cell binding hydrophilic polymeric side chains grafted to
the polymer backbone, wherein the side chains have a molecular
weight between 200 and 2000 Daltons; wherein between zero and 100%
of the non-cell binding, hydrophilic side chains are end-capped
with cell-binding or cell-signaling ligands to form short
cell-binding copolymer side chains and wherein the side chains
comprise less than 60% of the total copolymer weight, wherein the
comb copolymer is effective in regulating cellular adhesion or
response to the surface.
13. The tissue engineering matrix, cell culture matrix, biomedical
device or implant of claim 12 seeded with cells selected from the
group consisting of parenchymal cells, skin cells, muscle cells,
cartilage cells, nerve cells and bone cells.
14. The tissue engineering matrix, cell culture matrix, biomedical
device or implant of claim 12 wherein the cell-regulating,
comb-type copolymer comprises defined mixtures of non-cell binding
and ligand-modified cell-regulating, comb-type copolymers.
15. The tissue engineering matrix, cell culture matrix, biomedical
device or implan of claim 14, wherein the surface presents discrete
nanodomains or clusters of a single ligand type against a
background of non-cell binding hydrophilic side chains.
16. The tissue engineering matrix, cell culture matrix, biomedical
device or implant of claim 15, wherein each nanodomain or cluster
contains between 2 and 50 cell-signaling ligands in an area of
0.0001-0.01 microns square, with the overall spacing between the
edges of such domains in the range 3-200 nm.
17. The tissue engineering matrix, cell culture matrix, biomedical
device or implant of claim 14, wherein the surface presents
discrete nanodomains or clusters of two or more ligand types
against a background of non-cell binding hydrophilic side
chains.
18. The tissue engineering matrix, cell culture matrix, biomedical
device or implant of claim 17, wherein each nanodomain or cluster
contains between 2 and 50 cell-signaling ligands in an area of
0.0001-0.01 microns square, with the overall spacing between the
edges of such domains in the range 3-200 nm.
19. A method for making a tissue engineering matrix, cell culture
matrix, implant or biomedical device with regulated cellular
adhesion or response comprising coating or forming the matrix,
implant or device with a comb copolymer comprising: a) a
hydrophobic polymer backbone; b) non-cell binding hydrophilic
polymeric side chains grafted to the polymer backbone, wherein the
side chains have a molecular weight between 200 and 2000 Daltons;
wherein between zero and 100% of the non-cell binding, hydrophilic
side chains are end-capped with cell-binding or cell-signaling
ligands to form short cell-binding copolymer side chains and
wherein the side chains comprise less than 60% of the total
copolymer weight.
20. The method of making a tissue engineering matrix, cell culture
matrix, biomedical device or implant of claim 19 in which non-cell
binding side chains of the comb copolymers at the surface are
end-capped with ligands after the coating, matrix, device or
implant is formed.
21. A method for engineering tissue comprising growing cells on a
tissue-engineering matrix formed of or coated with the
cell-regulating, comb-type copolymer comprising: a) a hydrophobic
polymer backbone; b) non-cell binding hydrophilic polymeric side
chains grafted to the polymer backbone, wherein the side chains
have a molecular weight between 200 and 2000 Daltons; wherein
between zero and 100% of the non-cell binding, hydrophilic side
chains are end-capped with cell-binding or cell-signaling ligands
to form short cell-binding copolymer side chains and wherein the
side chains comprise less than 60% of the total copolymer weight,
wherein the comb copolymer is effective in regulating cellular
adhesion or response to the surface.
22. A polymer latex comprising polymer particles dispersed in
aqueous-containing media stabilized by cell-regulating, comb-type
copolymer comprising: a) a hydrophobic polymer backbone; b)
non-cell binding hydrophilic polymeric side chains grafted to the
polymer backbone, wherein the side chains have a molecular weight
between 200 and 2000 Daltons; wherein between zero and 100% of the
non-cell binding, hydrophilic side chains are end-capped with
cell-binding or cell-signaling ligands to form short cell-binding
copolymer side chains and wherein the side chains comprise less
than 60% of the total copolymer weight.
23. The polymer latex of claim 22 wherein the comb copolymers serve
as a stabilizing agent during the latex synthesis.
24. The polymer latex of claim 22 whereby the comb copolymer
comprises less than 1% of the latex dry weight.
25. The polymer latex of claim 22 further comprising
non-cell-binding comb copolymers whose side chains are not
end-capped with cell-binding or cell-signaling ligands.
26. The polymer latex of claim 22 further comprising latex
particles stabilized by non-cell binding comb copolymers to achieve
a defined mixture of non-cell binding and ligand-modified
cell-regulating latex particles.
27. The polymer latex of claim 22 in which non-cell binding side
chains of the comb copolymers at the surface of the particles are
end-capped with ligands after the latex particles are
synthesized.
28. Polymer coatings and films that regulate cell behavior prepared
by casting a polymer latex comprising polymer particles dispersed
in aqueous-containing media stabilized by cell-regulating,
comb-type copolymers comprising: a) a hydrophobic polymer backbone;
b) non-cell binding hydrophilic polymeric side chains grafted to
the polymer backbone, wherein the side chains have a molecular
weight between 200 and 2000 Daltons; wherein between zero and 100%
of the non-cell binding, hydrophilic side chains are end-capped
with cell-binding or cell-signaling ligands to form short
cell-binding copolymer side chains and wherein the side chains
comprise less than 60% of the total copolymer weight.
29. The polymer films and coatings of claim 28 prepared by casting
a mixed latex of microparticles stabilized with the non-cell
binding comb copolymers to achieve a defined mixture of non-cell
binding and ligand-modified cell-regulating latex particles.
30. The polymer films and coatings of claim 28 comprising discrete
domains of a single type of cell-signaling ligand against a
background of non-cell binding hydrophilic side chains, where the
domain size is on the order of the latex particles.
31. The polymer films and coatings of claim 30 wherein the domain
size is between 0.1 and 10 microns in diameter.
32. The polymer films and coatings of claim 28 comprising discrete
domains of multiple cell-signaling ligands against a background of
non-cell binding hydrophilic side chains, wherein the domain size
is on the order of the latex particles.
33. The polymer films and coatings of claim 32 where the domain
size is between 0.1 and 10 microns in diameter.
34. The polymer film or coating of claim 28 in which non-cell
binding side chains of the comb copolymers at the surface of the
applied film or coating are end-capped with ligands after the
coating or film is prepared.
35. A method of making polymer coatings and films that regulate
cell behavior comprising casting a polymer latex comprising polymer
particles dispersed in aqueous-containing media stabilized by
cell-regulating, combtype copolymers comprising: a) a hydrophobic
polymer backbone; b) non-cell binding hydrophilic polymeric side
chains grafted to the polymer backbone, wherein the side chains
have a molecular weight between 200 and 2000 Daltons; wherein
between zero and 100% of the non-cell binding, hydrophilic side
chains are end-capped with cell-binding or cell-signaling ligands
to form short cell-binding copolymer side chains and wherein the
side chains comprise less than 60% of the total copolymer
weight.
36. The method of making polymer film or coatings of claim 35
wherein non-cell binding side chains of the comb copolymers at the
surface are end-capped with ligands after the coating or film is
prepared.
Description
[0001] This application claims priority to U.S. Ser. No. 60/081,596
filed Apr. 13, 1998.
BACKGROUND OF THE INVENTION
[0003] Polymeric materials that elicit controlled cell responses,
and have good mechanical, optical and/or biodegradation properties,
are disclosed for use in biomedical applications. Processing
methods by which such polymers can be localized at a biomaterial
surface are also disclosed.
[0004] Polymers currently in use for biomedical applications
generally tend to be hydrophobic. As defined herein, hydrophobic
refers to a material that repels water, i.e., exhibits a static
contact angle with water greater than 60 degrees at 20.degree. C.,
and has a water permeability P less than 3.times.10.sup.-10
cm.sup.3(STP) cm/(cm.sup.2 s Pa). This can give rise to
uncontrolled interactions between cells and adsorbed proteins at
the surface of the material, which can result in a chronic
inflammatory response that can lead to failure of implants and even
promote tumorigenecity (Warson, The Applications of Synthetic Resin
Emulsions, Benn, London (1972)). Metal or ceramic materials used in
implant applications similarly can elicit undesirable cell
responses.
[0005] For tissue engineering applications, it is essential that
the polymeric material used to form a biodegradable scaffold for
cells promote cell adhesion, migration, growth and differentiation
while providing adequate structural support. Though commonly used
synthetic scaffold materials such as poly(lactide),
poly(glycolide), etc., and copolymers thereof, have suitable
mechanical, processing and biodegradation properties, their
hydrophobic nature leads to protein adsorption and denaturing on
the material surface which elicits uncontrolled cell response.
[0006] The ideal surface for many biomaterials applications would
resist protein adsorption while providing cells with specific
chemical signals to guide adhesion, survival, growth, migration and
differentiation. As used herein, the term "biomaterial" refers to a
nonviable material used in a medical device intended to interact
with biological systems. Polymer surfaces modified with
poly(ethylene oxide) have been studied in recent years for the
reduction of protein adsorption at the surface of biomaterials
(Paine et al. Macromolecules, 23:3104 (1990)). The objective of
these surface modification schemes is the elimination of
nonspecific interactions of cells with implant materials. One way
in which specific chemical signals can be relayed to cells at a
surface is through tethered ligands for cell surface receptors
(Barret, Brit. Polym. J. 5:259 (1973)). Delivery of signals in this
manner has advantages over the addition of soluble factors, as the
signal is presented in a very localized manner at a controlled dose
without diffusive loss (Kuhl and Griffith, Nature Medicine, 2:1002
(1996)). In addition, tethered ligands may provide more constant
stimulation to cells by avoiding the down-regulation present when
soluble ligands are internalized by cells. Control over spatial
distribution of ligands on surfaces may also be key to guiding cell
behavior. Thus systems which will allow spatial control of local
ligand density, or the creation of clusters of ligands on a
surface, in addition to providing control over the average surface
density of ligands, are highly desirable (Kornberg et al, Proc.
Natl. Acad. Sci. USA, 88:8392 (1991)).
[0007] Integrins, dimeric adhesion receptors including one of
approximately ten known alpha chains paired with one of
approximately six known beta chains, mediate a wide range of
interactions between cells and extracellular matrix (ECM) and
control cell behaviors as diverse as migration, growth, and
differentiation, providing a permissive environment for the action
of growth factors. For many integrins, the specificity of integrin
binding to matrix proteins has been mapped to small, discrete
peptide domains and new sites continue to be elucidated (Rouslahti,
Ann. Rev. Cell. Dev. Biol., 12:697 (1996); Hynes, Cell, 48:549
(1987)). The prototypical example of such specificity is the RGD
site first identified in fibronectin and subsequently identified in
other matrix proteins. The RGD peptide enables complete replacement
of adhesive finction of fibronectin for cells expressing certain
integrins.
[0008] Much data supports the idea that both occupancy and
clustering of integrins are required to elicit full cellular
responses mediated by integrins (Clark and Brugge, Science, 268:233
(1995)). For example, full EGFR activation of MAP kinase requires
integrin clustering and occupancy (Miyamoto et al, J. Cell Biol.,
135:1633 (1996)). Thus, the spatial presentation of ligand in the
environment, i.e., whether ligands are spaced closely enough to
afford clustering of ligand-bound integrins, may influence cellular
behaviors governed by integrins. Indeed, spacing of synthetic RGD
ligand covalently linked to the substrate has been shown to have an
influence on cell adhesion and spreading (Massia and Hubbell, J.
Cell Biol., 114:1089 (1991)). At the same time, the surface
concentration of an adhesion ligand such as fibronectin has been
shown to have a substantial influence on integrin-mediated
behaviors such as migration (DiMilla et al, J. Cell Biol., 122:729
(1993)). A recent study using self-assembled monolayers patterned
in one micron adhesive/nonadhesive domains demonstrated the role of
cell spreading and receptor occupancy on cell survival (Chen et al,
Science, 276:1425 (1997). The length scale in that study was
approximately that of a focal adhesion complex (or larger), but it
is likely that clustering over much smaller length scales (3-10
integrins) is also physiologically relevant. Indeed, data suggests
strongly that RGD clustering on the less than 100 nm length scale
has profound effects on the integrin-mediated behavior of
migration. Since both the concentration and spatial distribution of
ligand influence cell response, it is desirable to have a means to
vary these two parameters independently, and over a broad range of
length scales (nanometers to micrometers), in order to guide cell
response.
[0009] Integrins can initiate intracellular signaling cascades that
overlap with those of growth factors such as epidermal growth
factor (EGF). Cross-communication between adhesion and growth
factor receptors may occur by direct physical association within
the focal adhesions. Both types of receptors are concentrated in
these structures (Miyarnoto et al, J. Cell Biol., 135:1633 (1996);
Plopper et al, Mol. Biol. Cell, 6:1349 (1995)), and both receptors
can stimulate some of the same down-stream effect on molecules such
as MAP kinase. Close proximity of adhesion and growth factor
receptors in the focal adhesion complex provides for a free flow of
both positive and negative regulatory signals between the two. A
number of signaling molecules have been proposed as forming this
linkage; one intracellular mechanism of transmodulation is via
protein kinase C (PKC)-mediated attenuation of the epidermal growth
factor receptor (EGFR). It is also likely that PKC activity
secondary to phospholipase C.gamma. or phospholipase D activation
by EGFR alters integrin-based substratum connections (Welsh et al,
J. Cell Biol., 114:533 (1991); Ando et al, J. Cell. Physiol.,
156:487 (1993)). It is thus desirable to have a method by which two
or more types of signaling ligands, such as adhesion peptides and
growth factors, can be simultaneously located at the surface of a
biomaterial in controlled quanitity and spatial distribution.
[0010] To date, few if any model systems are able to meet both
protein resistance and cell signaling surface requirements, while
approaches using clinically-applicable materials have focused on
hydrogels (Hern and Hubbell, J. Biomed. Mater. Res., 39:266
(1998)), which have limited physical strength and are not suitable
for many applications. Other approaches for modifying the surfaces
of hydrophobic polymeric materials or other biomaterials to achieve
a more desirable surface composition for biomedical applications
include adsorption of block copolymers, chemical grafting of
polymers to the surface, and plasma deposition of an overlying
film. Each of these methods suffers various disadvantages. For
example, adsorbed block copolymers can be rearranged actively by
cells, grafted polymers are difficult to apply at high density on a
surface, and plasma deposition results in a gel-like surface
structure poorly suited for controlled cell signaling. None of
these methods provides a means for modifying the surface of complex
three-dimensional structures such as fibrous or sponge-like tissue
scaffolds, or for creating clustered ligand distributions of
variable concentration and spacing on biomaterial surfaces.
[0011] It would be advantageous to provide polymer materials and
processing methods that overcome the disadvantages of other
biomaterials surface modification approaches. It is therefore an
object of the present invention to provide polymer materials that
elicit controlled cell-surface interactions by inhibiting protein
adsorption, and, where appropriate, presenting controlled
concentrations and spatial distributions of cell-signaling ligands
on biomaterial surfaces. It is further an object of the present
invention to provide processing methods by which such polymers can
be placed at a biomaterial surface. It is further the object of the
present invention to provide polymeric materials which can be used
to create discrete nanometer- to micrometer-sized domains on a
biomaterial surface that present two or more different types of
ligands for regulating cellular response.
SUMMARY OF THE INVENTION
[0012] Comb-type copolymers that elicit controlled cellular
response, methods by which such polymers can be localized at a
surface, and methods of using such polymers for modifying the
surfaces of biomedical devices are disclosed.
[0013] The polymers include a hydrophobic, water-insoluble backbone
and low molecular weight, hydrophilic, non-cell binding side
chains. As defined herein, non-cell binding refers to materials
which exhibit no observable cell attachment after standard cell
culturing assays in serum containing media for 24 hours. The
molecular weight of the hydrophilic side chains is preferably above
200 Daltons and below 2000 Daltons. The backbone can be
biodegradable or non-biodegradable, depending on the intended
application. Biodegradable backbones are preferred for most tissue
engineering, drug delivery and wound healing device applications,
while non-biodegradable backbones are desirable for permanent
implant, biofiltration, and cell culture plate applications A
portion of the non-cell binding side chains can be end-capped with
cell-signaling ligands to control the degree of cell adhesion, or
other cell response, elicited by the polymer surface. In the
preferred embodiment, the overall comb copolymer should have a
molecular weight sufficiently high as to confer good mechanical
properties to the polymer in the melt state through chain
entanglements. That is, its molecular weight should be above the
entanglement molecular weight, as defined by one of ordinary skill
in the art. The overall molecular weight of the comb copolymer
should thus be above about 10,000 Daltons, more preferably above
20,000 Daltons, and more preferably still above 30,000 Daltons.
[0014] The density of the hydrophilic side chains along the
backbone of the copolymers depends on the length of the side chains
and the water-solubility characteristics of the final polymer. The
total percentage by weight of the hydrophilic side chains is
between 20 and 60 percent of the total copolymer composition,
preferably around 40 percent by weight. For combs incorporating
hydrophilic side chains with a molecular weight of about 350
Daltons, the mole percent of segments of the backbone carrying
hydrophilic side chains can be as high as 30 percent. For
hydrophilic side chains with a molecular weight of about 2000
Daltons, the mole percent of segments of the backbone carrying
hydrophobic side chains can be as low as 2 percent. In the
preferred embodiment, the overall comb copolymer is not
water-soluble. As defined herein, the term water-soluble refers to
materials having a solubility in aqueous solutions of greater than
1 gram per liter. When in contact with aqueous solutions, the
hydrophilic side chains swell and form a hydrated layer which
repels proteins and hence resists cellular adhesion.
[0015] The non-cell binding side chains of the comb copolymer can
be end-capped with cell-signaling chemical ligands in order to
elicit controlled cell responses Ligands such as adhesion peptides
or growth factors can be covalently or ionically attached to the
ends of the side chains using known chemistries to provide specific
chemical signals to cells. A defined fraction of ligand-bearing
side chains can be obtained by using appropriate stoichiometric
control during the coupling of the ligands to the polymers, by
protecting the end-groups on those side chains which are not to be
end-capped with ligands, or by combinations of these approaches.
For applications where it is desirable to cluster ligands on the
length scale of nanometers or tens of nanometers on a biomaterial
surface, more than one ligand (on average) can be covalently
attached to a single comb copolymer chain. In applications where it
is desirable to incorporate two or more types of ligands in a
single cluster on a biomaterial surface on the size scale of
nanometers to tens of nanometers, one or more of each of the ligand
types (for example, an adhesion peptide and growth factor) can be
attached to a single comb copolymer chain through its side chains
using known chemistries.
[0016] When adhesion peptides are coupled to the comb copolymer
side chains, cells attach and spread readily on the comb copolymer
surface. The amount of cell spreading and proliferation on the
surface therefore can be controlled by mixing adhesion
peptide-bearing comb copolymers with non-cell binding comb
copolymers, for example, so that less than 20% of the combs bear an
adhesion peptide. Similarly, the spatial distribution of ligand
clusters on the biomaterial surface can be controlled by mixing
non-cell binding comb copolymers with comb copolymers in which each
chain on average has more than one ligand attached to its side
chains In this case, the size of the ligand clusters (i.e., the
spatial area in which the ligands are localized) is dictated by the
characteristic size of the ligand-bearing comb copolymer, and can
be approximated from the comb copolymer's radius of gyration,
R.sub.G, which can be calculated or experimentally determined by
one of ordinary skill in the art. The comb copolymer radius of
gyration can range typically between nanometers and several tens of
nanometers, depending on total molecular weight, length of side
chains, and environment surrounding the polymer chain, for example,
other polymer chains or water molecules (P. -G. deGennes, Scaling
Concepts in Polymer Physics, Cornell University Press, 1979). Thus
the size of the ligand clusters, as well as the number and type of
ligands per cluster, can be controlled by the synthesis conditions
of the ligand-bearing comb copolymers. For example, a comb
copolymer with R.sub.G==4 nm would have an area per cluster of .pi.
R.sub.G.sup.2 or approximately 50 nm.sup.2. The number of clusters
on the surface per unit surface area (on average) can be controlled
by the ratio of ligand-bearing to non-cell binding combs at the
surface. To achieve a surface separation distance between ligand
clusters of d, where d>2R.sub.G, the concentration of
ligand-bearing combs should be approximately
.phi.=V.sub.chain/(2R.sub.Gd.sup.2), where V.sub.chain is the
volume occupied by a single comb copolymer chain. For example, to
achieve a cluster-to-cluster distance of 20 nm with a comb
copolymer which has R.sub.G=4 nm and V.sub.chain=48 nm.sup.3, the
estimated fraction of ligand-bearing combs required is 1.5 vol %. A
cluster-to-cluster distance of 10 nm would require 6 vol % of the
ligandbearing comb.
[0017] Numerous methods can be used to apply the comb copolymers,
or their mixtures, to various biomaterial surfaces. These methods
include dip coating, spray coating, brush coating, roll coating, or
spin casting a film onto the substrate, typically followed by mild
heating to promote adhesion to the surface. Solid free form
processes such as three dimensional printing techniques (3DP), or
freeze drying methods could be used to create complex
three-dimensional structures, including porous structures. In all
of these processing approaches a suitable crosslinking agent might
be incorporated to enhance the mechanical rigidity of the film or
device.
[0018] In applications where it is desirable to use only small
amounts of copolymer to modify the surface of a second, hydrophobic
or non-cell regulating polymer, the comb copolymers can be added in
small quantities to the second polymer and processed to achieve
segregation of the comb copolymer to the surface. In preferred
embodiments, the comb copolymer would comprise less than 10 wt % of
the polymer mixture. Processing steps to achieve segregation
include heating the mixture under a vacuum, in air, water, water
vapor, C0.sub.2 or other environment which favors the comb
component at the surface, at temperatures sufficiently above the
glass transitions of the polymer components to provide mobility for
achieving surface segregation. In the case where the second polymer
component is a semicrystalline polymer, the annealing temperature
should be above the glass transition but below the melting point of
the polymer, to ensure that the desired shape of the device is
retained. In preferred embodiments, surface segregation is achieved
during a standard processing step in the manufacture of a
biomedical device, such as during an extraction, autoclaving or
sterilization process. In other embodiments, segregation is
accomplished in an additional annealing step in a controlled
environment (water, etc), after device fabrication. Such processing
steps create a surface layer approximately 2R.sub.G in thickness
that contains almost exclusively the comb copolymer. The observable
surface properties of such annealed mixtures are substantially
identical to those of the pure comb copolymers. In preferred
embodiments, the comb copolymer is miscible with the second polymer
to avoid phase separation in the bulk device, which might lead to
poor mechanical or optical properties.
[0019] In other cases, the localization of the comb polymer to the
surface of a device primarily comprised of a second, hydrophobic or
non-cell regulating polymer can be accomplished during other steps
of device manufacture. For example, precise placement of the comb
copolymer at the surface of a device made from a second polymer can
be accomplished by 3DP methods. Likewise, differences in viscosity
between the comb copolymer and a second polymer when blended
together can be exploited to locate the comb to the surface during
melt extrusion of fibers, films or other devices. Porous or
nonporous membranes, films, fibers or hollow fibers in which the
comb copolymer resides at the surfaces can be prepared by phase
inversion casting.
[0020] In this method, a solution of the comb copolymer, the second
polymer, and a mutual solvent is cast into an aqueous-based
coagulation bath to form the device During the casting process,
favorable interactions between the comb and the coagulation bath
medium induce segregation of the comb copolymer to exterior
surfaces of the film, fiber, or membrane. Cell-regulating
microporous biodegradable membranes useful as temporary barrier
devices in wound-healing applications can be prepared in this
fashion Cell-regulating biodegradable sutures can similarly be
prepared by spinning fibers from solution into an aqueous-based
coagulation bath. Such surface-modified fibers can also be prepared
from biodegradable or nonbiodegradable materials and fashioned into
nonwoven fabric articles for biomedical applications including
cell-regulating temporary barrier devices and biofiltration
devices. Hollow nanoporous fibers can be prepared which have
cell-regulating interior surfaces. By encapsulating cells in a
portion of such a fiber, a long-term drug delivery implant could be
prepared which secretes desirable products of cells in quantities
regulated wholly or in part by tethered signals on the fiber inner
surface. Cell-regulating biodegradable microporous scaffolds with a
surface excess of comb copolymers can be prepared by freeze-drying
methods by choosing a sublimating solvent which has preferential
affinity to the comb copolymer component as compared to the second
polymer component which forms the bulk of the device.
[0021] In all cases described above where comb copolymers are used
in conjunction with a second polymer to prepare a device, the comb
copolymers can be non-cell binding combs, ligand-bearing combs, or
a mixture of these to achieve a desired cell response as previously
described.
[0022] A further method by which the comb copolymers can be used
for controlling cell response in biomedical applications is through
the preparation of polymer latexes that incorporate the comb
copolymers on the latex particle surfaces. Such latexes are
prepared by dispersion or emulsion polymerization methods in a
water-containing medium, using the comb copolymers as a stabilizing
agent. The polymerization is achieved by dissolving or mixing the
desired monomer, comb stabilizer and initiator in a
water-containing medium. The polymer is initiated, for example, by
applying heat to the solvent. The dispersion medium is a good
solvent for the comb copolymer but a poor solvent for the growing
polymer The hydrophobic comb backbone is chosen to be compatible
with the polymer being synthesized, and thus anchors to the surface
of the growing polymer particles, while the hydrophilic side chains
stabilize the particles against flocculation Upon completion of the
latex synthesis, the resulting latex particles are in the range of
0.1 to 10 .mu.m in size, typically dispersed at 20-70% polymer
solids by weight in the dispersion medium. These systems can be
employed in a variety of ways to control cell response through the
comb copolymers that remain anchored to the particle surfaces.
[0023] Films or coatings can be prepared from the latex dispersions
by usual methods such as dipping, brushing, rolling or casting the
latex onto any surface. For coatings applied to permanent implants
to control cell response, nonbiodegradable latex particles are
preferred, such as acrylics. Opaque coatings may be prepared that
elicit controlled cell response by employing any of the standard
coating methods used to form latex films by those skilled in the
art, such as those just mentioned. Alternatively, by heat-treating
films at a temperature well above the glass transition of the
polymer particles, the particles will coalesce into a smooth,
transparent film in which the comb copolymers reside at the
surface. The comb copolymers remain localized at the surface upon
coalescence due either to an energetic tendency to remain at the
surface, or because there is insufficient mobility for comb
diffusion into the bulk of the coalesced latex film, for example,
if the film is cooled below its glass transition shortly after
coalescence. The latex films exhibit surface properties akin to the
comb copolymers themselves, but have the advantages that only small
quantities of the comb copolymer are used (typically below 1 wt %
of the total latex), coatings can be easily applied from
water-based suspensions, and the film-forming properties can be
tailored to adhere to the substrate by judicious choice of the
film-forming polymer. For example, an acrylic latex stabilized by
non-cell binding comb copolymers could be used to prepare
transparent acrylic coatings on acrylic intraocular lenses in order
to render them resistant to cell attachment, and hence less subject
to clouding over time Acrylic latexes could also be used in
applications where controlled cell response is desired at the
surface of permanent metal, glass or ceramic implants or other
devices, including cell culture apparatus, since a high degree of
adhesion is often found between oxide surfaces and acrylic
polymers. For polystyrene cell culture plates or other apparatus, a
cell-regulating PS latex could be used to prepare a transparent,
cell-regulating coating in the manner described above.
[0024] In all cases described above where latexes are stabilized by
comb copolymers, the comb copolymers might be non-cell binding
combs, ligand-bearing combs, or a mixture of these to achieve a
desired cell response as previously described above. Alternatively,
mixed latex dispersions can be used to prepare films which contain
clustered ligand regions on a surface of sizes from 0.1 to 10
micrometers. This can be achieved by mixing together dispersions of
latex particles coated with non-cell binding combs and those coated
with ligand-bearing combs and creating films of these mixed
dispersions as described above. The size of the ligand clusters is
approximately the diameter of the latex particles coated with
ligand-bearing combs, while the number of clusters on the surface
per unit surface area can be controlled by the ratio of
ligand-bearing to non-cell binding latex particles in the mixed
dispersion.
[0025] For applications where a biodegradable film is preferred,
biodegradable latexes can be prepared using comb stabilizers with
biodegradable backbones. Such biodegradable latexes could also be
employed as drug delivery vehicles as described below.
DETAILED DESCRIPTION OF THE INVENTION
[0026] Comb-type copolymers that elicit regulated cellular
response, methods by which such polymers can be localized at a
surface, and methods of using such polymers for modifying the
surfaces of biomedical devices are disclosed.
[0027] These polymers are characterized by properties that are a
function of the type and ratio of hydrophilic side chains to
hydrophobic backbone polymers, type and number of tethered
cell-signaling ligands, molecular weight, and processing
conditions
[0028] I Polymer Composition
[0029] A. Polymer Architecture
[0030] The polymers are comb-type copolymers, with a backbone
formed of a hydrophobic, water-insoluble polymer and side chains
formed of short, hydrophilic non-cell binding polymers, having a
molecular weight of between 200 and 2000 Daltons. The hydrophobic
backbone can be biodegradable or non-biodegradable, depending on
the desired application. The overall comb copolymer should have a
molecular weight sufficiently high in the melt state as to confer
good mechanical properties to the polymer through chain
entanglement, that is, its molecular weight should be above the
entanglement molecular weight, as defined by one of ordinary skill
in the art. The overall molecular weight of the comb copolymer
should thus be above about 10,000 Daltons, more preferably above
20,000 Daltons, and more preferably still above 30,000 Daltons. The
comb copolymers can be prepared by copolymerizing a hydrophilic
macromonomer which contains a polymerizable chain end with a second
hydrophobic monomer. Alternatively, a hydrophobic monomer can be
copolymerized with a second monomer that includes suitable reactive
groups through which the hydrophilic side chains can be grafted to
the backbone. Alternatively, a hydrophobic monomer with a suitable
reactive side group can be polymerized and a fraction of those
reactive side groups can be modified by grafting hydrophilic side
chains. A defined percentage of the non-cell binding side chains
can be end-capped with a suitable ligand to elicit a specific
cellular response.
[0031] B. Hydrophobic Polymer Backbones
1. Biodegradable Hydrophobic Polymers
[0032] Hydrophobic polymers used to impart biodegradable properties
to the backbones of the comb copolymers are preferably hydrolyzable
under in vivo conditions. Suitable biodegradable polymeric units
include hydroxy acids or other biologically degradable polymers
that yield degradation products that are non-toxic or present as
normal metabolites in the body. These include poly(amino acids),
poly(anhydrides), poly(orthoesters), and poly(phosphoesters).
Polylactones such as poly(epsilon-caprolactone),
poly(delta-valerolactone), poly(gamma-butyrolactone)and poly
(beta-hydroxybutyrate), for example, are also useful. Preferred
poly(hydroxy acid)s are poly(glycolic acid), poly(DL-lactic acid)
and poly(L-lactic acid), or copolymers of poly(glycolic acid and
poly(lactic acid). In general, these materials degrade in vivo by
both non-enzymatic and enzymatic hydrolysis, and by surface or bulk
erosion.
[0033] Biodegradable regions can be constructed from monomers,
oligomers or polymers using linkages susceptible to biodegradation,
such as ester, peptide, anhydride, orthoester, and phosphoester
bonds.
2. Non-Biodegradable Hydrophobic Polymers
[0034] Representative non-biodegradable, hydrophobic polymers that
could be incorporated into the backbone of the comb copolymers
include polyalkylenes such as polyethylene and polypropylene,
polychloroprene, polyvinyl ethers, polyvinyl esters such as
poly(vinyl acetate), polyvinyl halides such as poly(vinyl
chloride), polysiloxanes, polystyrene, polyurethanes and copolymers
thereof, polyacrylates, such as poly(methyl (meth)acrylate),
poly(ethyl (meth)acrylate), poly(butyl(meth)acrylate),
poly(isobutyl (meth)acrylate), poly(hexyl(meth)acrylate),
poly(isodecyl (meth)acrylate), poly(lauryl (meth)acrylate),
poly(phenyl (meth)acrylate), poly(methyl acrylate), poly(isopropyl
acrylate), poly(isobutyl acrylate), and poly(octadecyl acrylate)
jointly referred to herein as "polyacrylates"), and copolymers and
mixtures thereof The polymers include useful derivatives, including
polymers having substitutions, additions of chemical groups, for
example, alkyl groups, alkylene groups, hydroxylations, oxidations,
and other modifications routinely made by those skilled in the
art.
[0035] Preferred non-biodegradable polymers include ethylene vinyl
acetate, polyacrylates, poly(chloroprene), and copolymers and
mixtures thereof
[0036] C. Non-cell Binding Hydrophilic Side Chains
[0037] The non-cell binding side chains are preferably
water-soluble when not attached to the backbone, and, more
preferably, are non-ionic. Suitable polymeric blocks include those
prepared from poly(ethylene glycol), poly(ethylene oxide),
partially or fully hydrolyzed poly(vinyl alcohol),
poly(vinylpyrrolidone), and dextran. Preferably, the side chains
are made from poly(ethylene glycol), poly(ethylene oxide), or
poly(acrylic acids).
[0038] The hydrophilic side chains may be intrinsically
biodegradable or may be poorly biodegradable or effectively
non-biodegradable in the body. In the latter two cases, the side
chains should be of sufficiently low molecular weight to allow
excretion. The preferred molecular weight range is below about 2000
Daltons, more preferably below 1000 Daltons, and most preferably,
below about 500 Daltons. When the polymer is polyethylene glycol,
it is preferred that the number of ethylene oxide monomeric units
is between about 4 and 20.
[0039] When double-bond containing monomers are used to prepare the
polymer backbone, a preferred method for incorporating the
hydrophilic side chains is to use a hydrophilic macromonomer with a
reactive double bond at one end which can be randomly incorporated
during free radical or other addition polymerization. An example of
such a macromonomer is PEG-methacrylate. The density of the
non-cell binding, hydrophilic side chains along the polymer
backbone is controlled by controlling the relative amounts of the
PEG-methacrylate or other suitable macromonomeric unit used.
[0040] In those embodiments in which the side groups are end capped
with cell-signaling ligands, appropriate functional groups, such as
--NH.sub.2, --OH, or COOH are included on the ends of the
macromonomers.
[0041] D. Monomers with Reactive Functional Groups
[0042] In many of the embodiments described herein, the monomers
used to form the polymer backbone include only two reactive groups,
both of which are reacted in order to form the polymer. For
example, lactic acid includes two reactive groups, a hydroxy group
and a carboxy group. --OH is the preferred reactive group. Although
the ends of a polylactic acid polymer include a hydroxy group and a
carboxy group, there are no reactive groups along the backbone in
the final polymer chain that can be used to form a comb
copolymer.
[0043] Monomers which contain one or more additional reactive
groups need to be incorporated into the polymer backbone,
preferably in a random fashion, in order to form the comb-type
copolymers when monomers that do not include these reactive groups
are used to prepare the polymer backbone. Examples of these types
of monomers are well known to those of skill in the art.
[0044] The requirements for a suitable reactive monomer are that it
can be incorporated in the growing polymer chain by participating
in the same types of chemical reactions as the growing polymer
chain. For example, when lactide is being polymerized using a Lewis
acid catalyst, a depsipeptide (cyclic dimer of an amino acid) can
be prepared from lysine, in which the epsilon amine group is
protected, for example, with a t-boc protecting group. The lysine
is incorporated into the polymer, and the protecting group can be
removed. The resulting amine groups are reactive with hydrophilic
polymers which include leaving groups such as tosylates,
tresylates, mesylates, triflates and other leaving groups well
known to those of skill in the art.
[0045] Alternatively, the reactive monomer can include a leaving
group that can be displaced with a nucleophilic group on a
hydrophilic polymer. For example, epichlorohydrin can be used
during the polymerization step. The monomer is incorporated into
the polymer backbone, and the chloride group is present on the
backbone for subsequent reaction with nucleophiles. An example of a
suitable hydrophilic polymer containing a nucleophilic group is a
PEG with a terminal amine group. PEG-NH.sub.2 can react with the
chloride groups on the polymer backbone to provide a desired
density of PEG-ylation on the polymer backbone. Using the chemistry
described herein, along with the general knowledge of those of
skill in the art, one can prepare polymer backbones which include
suitable leaving groups or nucleophiles for subsequent coupling
reactions with suitably functionalized hydrophilic polymers.
[0046] E. Ligands for Controlling Cell Response
[0047] A number of molecules are known to promote cell adhesion.
These can be amino acids, peptides or glycoproteins. Exemplary
cell-binding ligands include peptides possessing an
Arginine-Glycine-Aspartic acid (RGD) amino acid sequence or a
Tyrosine-Isoleucine-Serine-Arginine-Glycine (YISRG). The RGD
sequence, present in proteins such as fibronectin, has been shown
to be active in promoting cell adhesion and growth (Massia, S. P.
and Hubbell, J. A., J. Cell. Biol., 114:1089 (1991)). Incorporation
of RGD sequences at the ends of the copolymer side chains thus can
enhance cell adhesion and growth. This is particularly useful when
a substrate is not adhesive, for example, a polyester to which
cells such as hepatocytes show poor adhesion, which is then
modified with the comb copolymer to promote cellular adhesion in a
controlled manner.
[0048] Biologically active molecules may also be incorporated into
the copolymer to promote the adhesion and growth of a particular
cell type in vivo. Many growth factors are known and can be
obtained from commercial sources such as Sigma Chemical Co, St.
Louis, Mo., for example, growth factors including epidermal growth
factor, vascular endothelial growth factor, fibroblast growth
factor, etc.
[0049] F. Relative Ratios of Comb Components
1. Ratio of Hydrophilic to Hydrophobic Units
[0050] The density of the hydrophilic side chains along the polymer
backbone depends in part on the molecular weight of the side
chains. The total percent of the hydrophilic units to the
hydrophobic units in the comb copolymers is between 20 and 60
percent by weight, preferably around 40 percent by weight. For
hydrophilic side chains with a molecular weight of about 350, the
mole percent of backbone segments carrying hydrophilic side chains
can be as high as about 30 percent. For hydrophilic side chains
with a molecular weight of about 2000, the mole percent can be as
low as about 2 percent.
[0051] The relevant consideration when determining an appropriate
ratio of hydrophilic to hydrophobic units in the comb copolymers is
that the overall polymer, when the hydrophilic side chains are not
end-capped with cell-signaling ligands, has the defined non-cell
binding properties and preferably is not water-soluble. A
relatively high density of very short (MW 500 or less) hydrophilic
side chains can provide the same degree of resistance to cellular
adhesion as a lower density of higher molecular weight (for
example, a MW between 1500 and 2000) side chains. Those of skill in
the art can adjust the molecular weight and density of the polymers
taking these factors into consideration.
2. Density of Tethered Ligands
[0052] The non-cell binding side chains of the comb copolymers can
be end-capped with cell-signaling chemical ligands in order to
elicit specific cell responses. Ligands such as adhesion peptides
or growth factors can be covalently or ionically attached to the
ends of the side chains using known chemistries to provide specific
chemical signals to cells. A defined fraction of ligand-bearing
side chains can be obtained by using appropriate stoichiometric
control during the coupling of the ligands to the ends of the side
chains, by protecting the end-groups on those side chains which are
not to be endcapped with ligands, or by combinations of these
approaches. For applications where it is desirable to cluster
ligands on the length scale of nanometers or tens of nanometers on
a biomaterial surface, more than one ligand (on average) can be
attached to each comb copolymer chain. In applications where it is
desirable to incorporate two or more types of ligands in a single
cluster on a biomaterial surface on the size scale of nanometers to
tens of nanometers, one or more of each of the ligand types (for
example, an adhesion peptide and growth factor) can be attached to
each comb copolymer chain (on average) using known chemistries.
Presentation of the ligand (or ligands) at the surface can thus be
tailored in terms of overall surface density by exploiting the
multi-branch nature of the comb molecule, in terms of local
density, by the number of ligands attached to the same comb. The
ability of the polymers to control cellular adhesion or other cell
function can be adjusted by controlling the density of the
cell-signaling ligands presented at the surface.
[0053] II. Polymer Mixtures
[0054] A. Mixtures of Comb Copolymers
[0055] When adhesion peptides are coupled to the comb copolymer
side chains, cells attach and spread readily on the comb copolymer
surface. The amount of cell spreading and proliferation on the
surface therefore can be controlled by mixing adhesion
peptide-bearing comb copolymers with non-cell binding comb
copolymers, for example, so that less than 20%, more typically less
than 2%, of the combs bear an adhesion peptide. Similarly, the
spatial distribution of ligand clusters on the biomaterial surface
can be controlled by mixing non-cell binding comb copolymers with
comb copolymers in which each chain on average has more than one
ligand attached to its side chains.
[0056] The size of the ligand clusters (i.e., the spatial area in
which the ligands are localized) is dictated by the characteristic
size of the ligand-bearing comb copolymer, and can be approximated
from the comb copolymer's radius of gyration, R.sub.G, which can be
calculated or experimentally determined by one of ordinary skill in
the art. The comb copolymer radius of gyration can range typically
between nanometers and several tens of nanometers, depending on
total molecular weight, length of side chains, and environment
surrounding the polymer chain, for example, other polymer chains or
water molecules. Thus the size of the ligand clusters, as well as
the number and type of ligands per cluster, can be controlled by
the synthesis conditions of the ligand-bearing comb copolymers. For
example, a comb copolymer of R.sub.G would have an area per cluster
of .pi. R.sub.G.sup.2. The number of clusters on the surface per
unit surface area (on average) can be controlled by the ratio of
ligand-bearing to non-cell binding combs at the surface. To achieve
a surface separation distance between ligand clusters of d, where
d>2R.sub.G, the concentration of ligand-bearing combs should be
approximately .phi.=V.sub.chain/(2R.sub.Gd.sup.2), where
V.sub.chain is the volume occupied by a single comb copolymer
chain.
[0057] B. Mixtures of Comb Copolymers and Other Polymers
[0058] The copolymers described herein can be blended with other
polymers that do not elicit controlled cell responses. In
applications where it is desirable to use the comb copolymer to
modify the surface of a second, hydrophobic or non-cell regulating
polymer, the comb copolymer can be added in small quantities to the
second polymer and processed to achieve comb segregation to the
surface. Blends of the comb copolymers with other polymers include
those containing between 1 and 99% by weight of the comb
copolymers, preferably less than 20 wt % of the comb copolymers,
and more preferably less than 10 wt % of the comb copolymers.
Processing steps to achieve comb surface segregation include
heating the mixture under vacuum, in air, water, water vapor,
supercritical CO.sub.2 or other environment that favors the comb
component at the surface, at temperatures sufficiently above the
glass transitions of the polymer components (the matrix polymer and
the comb copolymer additive) to provide mobility for achieving
surface segregation. In the case where the second polymer component
is a semicrystalline polymer, the annealing temperature should be
above the glass transition but below the melting point of the
polymer, to ensure that the desired shape of the device is
retained.
[0059] In preferred embodiments, surface segregation is achieved
during a standard processing step in the manufacture of a
biomedical device, such as during an extraction, autoclaving or
sterilization process. In other embodiments, segregation is
accomplished in an additional annealing step in a controlled
environment (water, etc), after device fabrication. Such processing
steps create a surface layer approximately 2R.sub.G in thickness
that contains almost exclusively the comb copolymer. The observable
surface properties of such annealed mixtures are substantially
identical to those of the pure comb copolymers. In preferred
embodiments, the comb copolymer is miscible with the second polymer
to avoid phase separation in the bulk device, which might lead to
poor mechanical or optical properties.
[0060] In other cases, the localization of the comb polymer to the
surface of a device primarily comprised of a second, hydrophobic or
non-cell regulating polymer can be accomplished during other steps
of device manufacture. For example, precise placement of the comb
copolymer at the surface of a device made from a second polymer can
be accomplished by 3DP methods Likewise, differences in viscosity
between the comb copolymer and a second polymer when blended
together can be exploited to locate the comb to the surface during
melt extrusion of fibers, films or other devices. Porous or
nonporous membranes, films, fibers or hollow fibers in which the
comb copolymer resides at the surfaces can be prepared by phase
inversion casting. In this method, a solution of the comb
copolymer, the second polymer, and a mutual solvent is cast into an
aqueous-based coagulation bath to form the device. During the
casting process, favorable interactions between the comb and the
coagulation bath medium induce segregation of the comb copolymer to
exterior surfaces of the film, fiber, or membrane. Cell-regulating
microporous biodegradable membranes useful as temporary barrier
devices in wound-healing applications can be prepared in this
fashion. Cell-regulating biodegradable sutures can similarly be
prepared by spinning fibers from solution into an aqueous-based
coagulation bath. Such surface-modified fibers can also be prepared
from biodegradable or nonbiodegradable materials and fashioned into
nonwoven fabric articles for biomedical applications including
cell-regulating temporary barrier devices and biofiltration
devices. Hollow nanoporous fibers can be prepared which have
cell-regulating interior surfaces. By encapsulating cells in a
portion of such a fiber, a long-term drug delivery implant could be
prepared which secretes desirable products of cells in quantities
regulated wholly or in part by tethered signals on the fiber inner
surface. Cell-regulating biodegradable microporous scaffolds with a
surface excess of comb copolymers can be prepared by freeze-drying
methods by choosing a sublimating solvent which has preferential
affinity to the comb copolymer component as compared to the second
polymer component which forms the bulk of the device.
[0061] In all cases described above where comb copolymers are used
in conjunction with a second polymer to prepare a device, the comb
copolymers can be non-cell binding combs, ligand-bearing combs, or
a mixture of these to achieve a desired cell response as previously
described. The observable surface properties of the device are
substantially identical to those of the comb copolymer or comb
copolymer mixture itself. ps III. Latexes Prepared with Comb
Copolymers
[0062] A. LatexSynthesis
[0063] A further method by which the comb copolymers can be used
for controlling cell response in biomedical applications is through
the preparation of polymer latexes that incorporate the comb
copolymers on the latex particle surfaces. Such latexes can be
prepared by dispersion or emulsion polymerization methods in a
water-containing medium, using the comb copolymers as a stabilizing
agent. The polymerization is achieved by dissolving the desired
monomer, comb stabilizer and initiator in a water-containing
medium. The polymer is initiated, for example, by applying heat to
the solvent. The dispersion medium is a good solvent for the comb
copolymer but a poor solvent for the growing polymer. The
hydrophobic comb backbone is chosen to be compatible with the
polymer being synthesized, and thus anchors to the surface of the
growing polymer particles, while the hydrophilic side chains
stabilize the particles against flocculation. Upon completion of
the latex synthesis, the resulting latex particles are in the range
of 0.1 to 10 .mu.m in size, typically dispersed at 20-70% polymer
solids by weight in the dispersion medium. These systems can be
employed in a variety of ways to control cell response through the
comb copolymers that remain anchored to the particle surfaces.
[0064] Polymers which might be synthesized as latex particles for
non-biodegradable applications include polyvinyl ethers, polyvinyl
esters such as poly(vinyl acetate), polyvinyl halides such as
poly(vinyl chloride), polystyrene, and polyacrylates, such as
poly(methyl (meth)acrylate), poly(ethyl (meth)acrylate),
poly(butyl(meth)acrylate), poly(isobutyl (meth)acrylate),
poly(hexyl(meth)acrylate), poly(isodecyl (meth)acrylate),
poly(lauryl (meth)acrylate), poly(phenyl (meth)acrylate),
poly(methyl acrylate), poly(isopropyl acrylate), poly(isobutyl
acrylate), and poly(octadecyl acrylate), and copolymers and
mixtures thereof, as well as useful derivatives of these polymers,
including polymers having substitutions, additions of chemical
groups, for example, alkyl groups, alkylene groups, hydroxylations,
oxidations, and other modifications routinely made by those skilled
in the art.
[0065] Polymers which might be synthesized as latex particles for
biodegradable applications include poly(amino acids),
poly(anhydrides), poly(orthoesters), and poly(phosphoesters),
polylactones such as poly(epsilon-caprolactone),
poly(delta-valerolactone), poly(gamma-butyrolactone)and poly
(beta-hydroxybutyrate), and poly(hydroxy acid)s such as
poly(glycolic acid), poly(DL-lactic acid) and poly(L-lactic acid),
or copolymers of poly(glycolic acid and poly(lactic acid).
[0066] B. Latex Films
[0067] Films or coatings can be prepared from the latex dispersions
by usual methods such as dipping, brushing, rolling or casting the
latex onto any surface. For coatings applied to permanent implants
to control cell response, non-biodegradable latex particles
prepared with non-biodegradable comb stabilizers are preferred. For
applications where a biodegradable film is preferred, biodegradable
latexes can be prepared using comb stabilizers with biodegradable
backbones. Opaque coatings may be prepared that elicit controlled
cell response by employing any of the standard coating methods used
to form latex films, such as those just mentioned. Alternatively,
by heat-treating films at a temperature well above the glass
transition of the polymer particles, the particles will coalesce
into a smooth, transparent film in which the comb copolymers reside
at the surface. The comb copolymers remain localized at the surface
upon coalescence due either to an energetic tendency to remain at
the surface, or because there is insufficient mobility for comb
diffusion into the bulk of the coalesced latex film, for example,
if the film is cooled below its glass transition shortly after
coalescence.
[0068] The latex films exhibit surface properties of the comb
copolymers themselves, but have the advantages that only small
quantities of the comb copolymer are required (typically below 1 wt
% of the total latex), coatings can be easily applied fi-om
water-based suspensions, and the film-forming properties can be
tailored to adhere to the substrate by judicious choice of the
film-forming polymer. For example, an acrylic latex stabilized by
non-cell binding comb copolymers could be used to prepare
transparent acrylic coatings on acrylic intraocular lenses in order
to render them resistant to cell attachment, and hence less subject
to clouding over time. Acrylic latexes could also be used in
applications where controlled cell response is desired at the
surface of permanent metal, glass or ceramic implants or other
devices, including cell culture apparatus, since a high degree of
adhesion is often found between oxide surfaces and acrylic
polymers. For polystyrene cell culture plates or other apparatus, a
cell-regulating PS latex could be used to prepare a transparent,
cell-regulating coating in the manner described above.
[0069] In all cases described above where latexes are stabilized by
comb copolymers, the comb copolymers might be non-cell binding
combs, ligand-bearing combs, or a mixture of these to achieve a
desired cell response as previously described above. Alternatively,
mixed latex dispersions can be used to prepare films that contain
clustered ligand regions on a surface of sizes from 0.1 to 10
micrometers. This can be achieved by mixing together dispersions of
latex particles coated with non-cell binding combs and those coated
with ligand-bearing combs and creating films of these mixed
dispersions as described above. The size of the ligand clusters is
approximately the diameter of the latex particles coated with
ligand-bearing combs, while the number of clusters on the surface
per unit surface area can be controlled by the ratio of
ligand-bearing to non-cell binding latex particles in the mixed
dispersion.
[0070] IV. Polymer Preparation
[0071] Methods for preparing hydrophobic polymers including
reactive monomeric units are known. Typical reactions are ring
opening polymerization (for monomers such as lactide, glycolide,
and other cyclic monomeric units), free radical polymerization (for
double bond-containing monomeric units such as methyl
methacrylate), and anionic or other addition polymerizations.
[0072] The monomers used to prepare the hydrophobic polymer
backbone, for example, lactide, glycolide, caprolactone, and
trimethylene carbonate, can be reacted with various polymerization
initiators, for example, alcohols such as ethylene glycol and
ethanol, water, and amines, in the presence of a suitable catalyst
such as a Lewis acid, as described, for example, in Kricheldorf, H.
R in Models of Biopolymers by Ring-Opening Polymerization, Penczek,
S., Ed., CRC Press, Boca Raton, 1990, Chapter 1; Kricheldorf, H. R
.alpha.-Aminoacid-N-Carboxy-Anhydrides and Related Heterocycles,
Springer-Verlag, Berlin, 1987; and Imanishi, Y. in Ring-Opening
Polymerization, Ivin, K. J. and Saegusa, T., Eds., Elsevier,
London, 1984, Volume 2, Chapter 8.
[0073] The cell-binding polymer side chains grafted onto the
polymer backbone are preferably hydrophilic polymers, such as
polyethylene glycol, polyethylene oxide, polyacrylic acid, dextran
and mixtures thereof, which can be modified to include reactive
functional groups such as amino, carboxylic acid, halo, sulfide,
guanidino, imidazole and hydroxyl groups. These groups can react
with various reactive groups on the polymer backbone in routine
nucleophilic displacement reactions to graft the hydrophilic
polymers to the backbone. The side chain polymers can be end-capped
with cell binding ligands through standard covalent or ionic
coupling reactions.
[0074] V. Surface Coatings and Devices incorporating Comb
Copolymers
[0075] Numerous methods can be used to apply the comb copolymers,
comb copolymer mixtures, or mixtures of comb copolymers and other
polymers to surfaces. These methods include dip coating, spray
coating, brush coating, roll coating, or spin casting a film onto
the substrate followed by mild heating to promote adhesion to the
surface. Solid free form processes such as 3DP, or freeze drying
methods could be used to create complex three-dimensional
structures, including porous structures. In all of these processing
approaches a suitable crosslinking agent might be incorporated to
enhance the mechanical rigidity of the coating or device.
[0076] In applications where mixtures of comb copolymers with other
polymers are desirable, processing steps to achieve comb surface
segregation include heating the mixture under vacuum, in air,
water, water vapor, supercritical CO.sub.2 or other environment
that favors the comb component at the surface, at temperatures
sufficiently above the glass transitions of the polymers to provide
the combs with the necessary mobility. In the case where the second
polymer component is a semicrystalline polymer, the annealing
temperature should be above the glass transition but below the
melting point of the polymer, to ensure that the desired shape of
the device is retained.
[0077] Surface segregation could be achieved preferably during a
standard processing step in the manufacture of a biomedical device,
such as during an extraction, autoclaving or sterilization process,
or could be accomplished in a separate annealing step after the
device has been manufactured. This type of processing creates a
surface layer on the device that contains almost exclusively the
comb copolymer. In other cases, the localization of the comb
polymer to the surface of a device primarily comprised of a second
polymer can be accomplished during other steps of device
manufacture. For example, differences in viscosity between the comb
copolymer and a second polymer when blended together can be
exploited to locate the comb to the surface during melt extrusion
of fibers, films or other devices. Porous or nonporous membranes,
films, fibers or hollow fibers in which the comb copolymer resides
at the surfaces can be prepared by phase inversion casting. In this
method, a solution of the comb copolymer, the second polymer, and a
mutual solvent is cast into an aqueous-based coagulation bath to
form the device. During the casting process, favorable interactions
between the comb and the coagulation bath medium induce segregation
of the comb copolymer to exterior surfaces of the film, fiber, or
membrane. Cell-regulating microporous biodegradable membranes
useful as temporary barrier devices in wound-healing applications
can be prepared in this fashion. Cell-regulating biodegradable
sutures can similarly be prepared by spinning fibers from solution
into an aqueous-based coagulation bath. Such surface-modified
fibers can also be prepared from biodegradable or nonbiodegradable
materials and fashioned into nonwoven fabric articles for
biomedical applications including cell-regulating temporary barrier
devices and biofiltration devices. Hollow nanoporous fibers can be
prepared which have cell-regulating interior surfaces. By
encapsulating cells in a portion of such a fiber, a long-term drug
delivery implant could be prepared which secretes desirable
products of cells in quantities regulated wholly or in part by
tethered signals on the fiber inner surface. Cell-regulating
biodegradable microporous scaffolds with a surface excess of comb
copolymers can be prepared by freeze-drying methods by choosing a
sublimating solvent which has preferential affinity to the comb
copolymer component as compared to the second polymer component
which forms the bulk of the device.
[0078] V. Biomedical Applications
[0079] The comb-type copolymers described herein may be used in a
variety of biomedical applications, such as in scaffolds and
supports for cell growth in tissue engineering, coatings for
biomedical implants such as intraocular lenses or other permanent
implants made from polymeric, metal, glass, or ceramic materials,
and coatings for cell culture apparatus such as cell culture
plates, pipets, etc.. The comb-type copolymers may be used for
modifying the surface properties of sutures, temporary barrier
films or fabrics in wound-healing applications, artificial hearts
and blood vessels, catheters, filters for blood or other body
fluids, and targeted controlled-release drug delivery vehicles and
encapsulated cell drug delivery systems. The materials are
preferably biodegradable when used for tissue engineering, wound
healing, and targeted drug delivery applications, and are
preferably non-degradable when used to modify implants, cell
culture apparatus, filtration devices, and other devices intended
for long term use or implantation.
[0080] A. Tissue Engineering
[0081] For use in tissue engineering applications, the comb
copolymers may be derivatized by the attachment to the ends of the
hydrophilic side chains biologically active molecules that promote
favorable cell-polymer interactions, such as cell adhesion
molecules and growth factors. Matrices suitable for seeding or
ingrowth of cells can be formed which incorporate the comb
copolymers, or a matrix formed of a material such as stainless
steel, collagen, or another polymer can be coated with the comb
copolymers. The matrix is then either seeded with cells and
implanted, or the matrix implanted for tissue ingrowth to occur.
These materials can be tailored to fit the particular needs of a
variety of cell types through changes in the type and density of
cell adhesion peptides attached to the copolymers. Cell types which
can be seeded on the matrices include parenchymal cells such as
hepatocytes, uroendothelial cells, skin cells, muscle cells, nerve
cells and bone and/or cartilage forming cells. Normal cells, fetal
cells or genetically engineered cells can be seeded onto the
matrices.
[0082] B. Drug Delivery and Imaging
[0083] The comb copolymers also may be formed into matrices for use
as drug delivery systems or for imaging purposes. Biodegradable
latexes coated with the comb copolymers be can be used for targeted
delivery of a therapeutic, prophylactic or diagnostic agent. Hollow
nanoporous fibers can be prepared which have cell-regulating
interior surfaces comprised of comb copolymers or comb copolymer
mixtures. By encapsulating cells in a portion of such a fiber, a
long-term drug delivery implant could be prepared which secretes
desirable products of cells in quantities regulated wholly or in
part by tethered signals on the fiber inner surface.
[0084] For use in drug delivery, a therapeutic or prophylactic
agent, such as an amino acid, bioactive peptide or protein,
carbohydrate, sugar, or polysaccharide, nucleic acid or polynucleic
acid, synthetic organic compound, or metal may be attached to
through the end groups of the hydrophilic side chains of the comb
copolymer using methods available in the art. The comb copolymers
may be modified to increase the level of the incorporated agent.
Agents which provide greater stability for the agent to be
delivered may be covalently or ionically attached to the copolymer.
The comb copolymers may be functionalized with a specific binding
moiety, e.g, an antibody, which targets the latex particle for
delivery to a particular site within the body Hydrophilic,
hydrophobic, acidic, basic or ionic side chains also may be
attached to the copolymers to expand their use as delivery devices
for drugs. Matrices of the modified drug-containing comb copolymer
may be administered to an animal orally or parenterally to deliver
the drug to the animal in vivo at a site in the animal where it is
needed.
[0085] Diagnostic agents include radioactive materials, fluorescent
materials, enzymatic materials, gases, and magnetic materials.
[0086] C. Use of the Materials to Provide Cell Repulsive
Surfaces
[0087] It is often desirable to minimize cell and tissue
interactions with biomedical implants, such as intraocular lenses.
These interactions are minimized when the surface of an implant is
coated with the non-cell binding copolymers. It is preferred that
the copolymer be non-degradable in some applications. For example,
when intraocular lenses are implanted, they are intended to remain
in place for extended periods of time and biodegradability is to be
avoided.
[0088] A preferred non-biodegradable polymeric material is a
copolymer of an alkyl acrylate (i.e., methyl methacrylate) and
PEG-methacrylate. A preferred method to place this coating at the
surface is through the formation of a latex film.
[0089] The present invention will be further understood by
references to the following non-limiting examples, in which the
following materials and equipment were utilized.
Example 1: Preparation, Processing, and Evaluation of Biodegradable
Comb Copolymers and Their Blends
[0090] Comb Polymer Synthesis
[0091] Lactide, epichlorohydrin, poly(ethylene glycol) methyl ether
(MPEG, M.sub.W.about.350 g/mole), poly(ethylene glycol) (PEG,
M.sub.W.about.400 g/mole), and anhydrous toluene (all from Aldrich
Chemical Co.) were used as received.
[0092] Tetrahydrofuran (Aldrich Chemical Co.) was distilled prior
to use. Lactide and epichlorohydrin (Aldrich Chemical Co ) were
copolymerized by ring opening polymerization (Shen et al. J. Polym.
Sci., Polym. Chem. Ed., 31:1393 (1993)) at 100.degree. C. in
toluene with a trioctyl aluminum-water catalyst. The in situ
AlOct.sub.3:0.5 H.sub.2O catalyst was prepared using a modification
of a literature procedure. Briefly, AlOct.sub.3 (25 wt % in hexane,
Aldrich Chemical Co.) and distilled THF were stirred in a sealed
flask under nitrogen and allowed to equilibrate at -68.degree. C.
in a dry ice/acetone bath. H.sub.2O was added to the mixture to
give a 1:0.5 molar ratio between AlOct.sub.3 and water. The mixture
was stirred vigorously at -68.degree. C. for 15 minutes, then
removed from the dry ice bath and allowed to return to room
temperature over 30 minutes. The catalyst solution was then
injected into a sealed reaction flask containing lactide,
epichlorohydrin, and toluene under nitrogen and allowed to react 16
hours at 100.degree. C. The resulting LA-EO copolymer was purified
by repeated precipitation in petroleum ether.
[0093] Grafting of MPEG and PEG to the LA-EO copolymer was
performed by phase transfer catalysis (Ober, Makromol. Chem.,
Macromol. Symp. 35:36-87(1990)) reacting the terminal hydroxyls of
the ethylene glycol chains with the pendant chlorine groups of the
backbone copolymer. The LA-EO copolymer was dissolved in methylene
chloride. PEG, MPEG, and pH 8 aqueous NaHCO.sub.3 were then added
with vigorous stirring. The mixture was allowed to react overnight.
Unreacted glycols were removed from the polymer by repeated
precipitations in methanol. The final non-cell binding comb
copolymer had a molecular weight of approximately 40,000 Daltons,
was insoluble in water, and incorporated approximately 40% by
weight hydrophilic PEG side chains.
[0094] A pentamer amino acid sequence, Gly-Arg-Gly-Asp-Ser-Pro
(GRGDSP from Gibco, referred to herein as RGD), was used to create
adhesion ligand-bearing comb copolymers by tethering the RGD to
functionalized ends of PEG side chains. RGD interacts specifically
with receptors known as integrins on the surface of cell membranes,
and RGD-integrin coupling mediates adhesion of cells to their
surroundings in vivo. RGD was coupled to the non-cell binding comb
copolymers via primary amines using known tresyl chloride chemistry
(Obel, et al. J. Polym. Sci., Polym. Lett. Ed. 23:103 (1985)). Comb
copolymers were activated with tresyl chloride groups in solution
and stored at -20.degree. C. until use. RGD was coupled to the
combs by immersion in PBS solutions of RGD (25 .mu.g/mL, pH 7.4) at
5.degree. C. for 3 hours. Systems were multiply rinsed with PBS to
remove unreacted RGD.
[0095] Film Processing
[0096] Mixtures of the activated comb copolymers and the non-cell
binding comb copolymers were prepared in various ratios, and cast
from solution in toluene onto glass slides. Films were subsequently
dried under vacuum for 24 hours to remove residual solvent. RGD was
subsequently subsequently coupled to the exposed activated comb
polymers at the surface of the films in the manner described above.
For mixtures containing 100 wt %, 25 wt % and 5 wt %
tresyl-activated comb copolymer, surface RGD densities of 9.5
pg/cm.sup.2, 2.5 pg/cm.sup.2, and 0.5 pg/cm.sup.2 were achieved,
respectively.
[0097] Mixtures of polylactide, PLA, homopolymer and small amounts
of the non-cell binding or RGD-bearing comb copolymers (10 wt % or
less relative to PLA) were dissolved in toluene, and cast as films
on glass. Films were subsequently dried under vacuum for 24 hours
to remove residual solvent. Some of the comb/PLA films were
subsequently annealed 96 hours in a 70.degree. C. water bath. X-ray
photoelectron spectroscopy studies showed significant enrichments
of the comb copolymer at the surface of annealed blends (.about.60%
by volume comb copolymer at the surface for a 10% bulk
concentration). Advancing/receding contact angle measurements
similarly indicate that the annealed blend films have substantially
lower water contact angles than PLA and exhibit a large hysteresis
indicative of PEG side chain reorientation/hydration at the surface
when in contact with water.
[0098] Cell Culture
[0099] NR6 fibroblasts were cultured in serum-containing media onto
the mixed comb films, the comb/PLA blends, and a PLA control film.
Polymer films were first sterilized by immersion in ethanol
Wild-type NR6 fibroblasts were seeded on polymer film surfaces in
Modified Eagle's Medium supplemented with fetal bovine serum. Cells
were cultured for 24 hours, media was aspirated, and fresh media
applied before phase contrast photomicrographs were taken.
[0100] Films of the non-cell binding combs were completely
resistant to cell adhesion over 24 hour time periods, even in the
presence of serum-supplemented medium. This is believed to be due
to the formation of a dense hydrated layer of PEG side chains at
the film surface. In the mixed comb films, increasing surface
densities of RGD increased the adhesion and spreading of cells on
the film surface. Variation in the weight fraction of RGD-coupled
combs in films with unfunctionalized combs from 0 to 100% allowed a
change in the adhesive response of cells to the surfaces. At 0%
RGD-combs, no cells adhered, at 5% RGD-combs cells stuck but
retained a rounded morphology, and at 100% RGD-combs cells were
strongly adhered and spread on the surfaces.
[0101] This result demonstrates that comb mixtures can provide some
level of tunable ligand presentation and control over cell
adhesion. The RGD-bearing surfaces supported cell adhesion and
spreading even in the absence of serum. In addition, soluble RGD
added to media abrogated the spreading of cells and detached them
from the surfaces. These results indicate that the effects seen are
due to specific interactions between cellular integrins and RGD and
not interactions between integrins and serum proteins adsorbed on
RGD.
[0102] On the surface of annealed PLA/non-cell binding comb blends,
no cell adhesion was found due to the formation of the
comb-enriched surface layer which resists protein adsorption. By
comparison, cell adhesion was observed on unmodified PLA and, to a
lesser degree, on the unannealed blend, which both allowed cells to
stick and spread in an uncontrolled fashion. Cell culture studies
on annealed PLA/RGD-bearing comb blends showed significant
controlled cell attachment through the RGD ligands, even in the
absence of serum.
[0103] Modulation of the degree of cell adhesion was also
demonstrated in cell culture experiments with primary rat
hepatocytes. The cells were plated at a density of 30,000
cell/cm.sup.2 on substrates containing either 1% or 100%
RGD-bearing comb in a 10% comb/PLA blend film, prepared and
annealed as described above. Hepatocytes remain highly spread on
substrates containing 100% RGD combs, but aggregate and assume a
spheroidal morphology on blends in which only 1% of the combs were
RGD-bearing.
Example 2: Preparation of Biodegradable Devices from Comb/PLA
Blends
[0104] Porous Scaffold
[0105] Biodegradable PLA/comb copolymer microporous scaffolds that
might be used as substrates for tissue engineering applications
were prepared by freeze drying solutions of 10% wt/vol polymer in
dioxane. Blends-containing 10 wt % of the biodegradable, non-cell
binding comb copolymer and 90 wt % PLA were dissolved in dioxane
and frozen in liquid nitrogen, causing the phase separation of the
polymer and solvent. Upon sublimating the dioxane, a porous
biodegradable foam was obtained, which could be further treated,
for example, by autoclaving or heat-treating in deionized (Dl)
water at 90.degree. C., to achieve a high coverage of the comb
copolymer on the pore exterior surfaces.
[0106] Temporary Barrier Membrane
[0107] Biodegradable PLA/comb copolymer microporous membranes that
might be used as temporary barriers in wound healing applications
were prepared by phase inversion casting from solutions of 10-20%
polymer in N,N-dimethylformamide (DW). Blends containing 10 wt % of
the biodegradable, non-cell binding comb copolymer and 90 wt % PLA
were dissolved in DMF and cast using a doctor blade onto a cleaned
glass substrate. The substrate was immediately immersed in a bath
of deionized (DI) water at 90.degree. C. to create a porous
membrane structure during the precipitation of the insoluble
polymer. Once formed, the membranes were removed and rinsed in a
second DI bath at 90.degree. C. to remove trace solvent
impurities.
Example 3: Preparation and Evaluation of Non-Biodegradable Comb
Copolymers and Their Mixtures
[0108] Comb Synthesis
[0109] Non-biodegradable comb polymers were synthesized by free
radical polymerization of methyl methacrylate (MMA) with either
methoxy poly(ethylene glycol) methacrylate (MPEGMA) or
poly(ethylene glycol) methacrylate (PEGMA) or a mixture of these
initiated in toluene at 70.degree. C. by azo(bis)isobutyronitrile.
After 12-16 hours the reaction was terminated, and the polymer
precipitated in petroleum ether. The resulting comb polymer has a
PMMA backbone with PEO side chains nearly randomly distributed
along the backbone, and a molecular weight of approximately 20,000
g/mole. The PEGMA macromonomers provide side chains end-capped with
a hydroxyl group which can be derivatized for covalent linkage of
the peptides, while the MPEGMA units provide non-reactive
methoxy-terminated PEG side chains. Combs containing .about.40% PEG
side chains by weight are insoluble in water but form very
hydrophilic, protein- and cell-resistant surfaces, and thus are
considered non-cell binding
[0110] To obtain adhesion ligand-bearing non-biodegradable combs,
the RGD peptide was attached to hydroxyl end groups of the PEG side
chains. The combs were dissolved in anhydrous tetrahydrofuran
(THF), followed by addition of triethylamine and tresyl chloride,
and reacted for 90 minutes. The activated polymer was recovered by
precipitation in anhydrous methanol, and stored at -70.degree. C.
until use. RGD was coupled through primary amines to the activated
combs by first dissolving the combs in dry THF, followed by
addition of peptide solution (1 mg/mL GRGDSP in phosphate buffered
saline (PBS)) at a ratio of 10:1 THF:PBS. Coupling was allowed to
proceed with stirring for 3 hours at 5.degree. C. The resulting
RGD-comb polymer was recovered by precipitation/washing with
deionized water.
[0111] Film Preparation and Cell Culture
[0112] Films for cell culture were prepared by spin-coating the
comb polymers onto glass substrates from anhydrous toluene. Purely
cell-resistant surfaces were prepared by spin-coating solutions of
the non-cell binding combs, while ligand-bearing surfaces were made
by spin-coating solutions containing both non-cell binding combs
and RGD-bearing combs.
[0113] NR6 fibroblasts transfected with the wild-type human
epidermal growth factor receptor (WT NR6) were cultured in modified
Eagle's medium alpha (MEM-.alpha.) supplemented with 7.5% fetal
bovine serum, L-glutamine, non-essential amino acids, sodium
pyruvate, penicillin-streptomycin, and gentamycin antibiotic. Cell
were seeded at 20,000 cells/cm.sup.2 onto comb copolymer films for
24 hours, followed by aspiration to remove unattached cells and
application of fresh medium. Morphology/adhesion of cells to films
was then assessed using a Zeiss Axiovert 100 phase contrast
microscope. No cell adhesion was observed on films of non-cell
binding combs. In contrast, films of the RGD-bearing combs
supported adhesion and produced cell morphologies comparable to
that observed on fibronectin.
Example 4: Preparation and Evaluation of EGF-Tetliered Comb
Films
[0114] Film Preparation
[0115] To obtain non-biodegradable combs with tethered epidermal
growth factor ligands, non-cell binding combs with PMMA backbones
and PEG side chains were prepared as described in Example 3. EGF
was attached to hydroxyl end groups of the PEG side chains by first
activating the side chains with tresyl chloride following the
procedure described above.
[0116] Films of the tresyl-activated comb were spin-coated at 1000
rpm from 0.01 g/ml toluene solutions. Films were subsequently dried
under vacuum to remove residual solvent, then sterilized by UV
exposure for 1 hour. EGF was coupled to surfaces by incubating 5
.mu.g/ml sterile PBS solutions (100 mM phosphate) of EGF on the
films for 3 hours at 5.degree. C. Solutions were aspirated and
samples were blocked with 100 mM pH7 sterile tris solutions 1 hour
at 20.degree. C. Controls were hydrolyzed in the presence of tris,
thereby capping all the tresyl sites with tris instead of EGF. One
hydrolyzed control was exposed to an EGF solution under conditions
simulating the EGF coupling step to check for nonspecific
adosrption of EGF: Samples were multiply rinsed with sterile PBS.
This protocol provided 1.0.+-.10.3 ng/cm.sup.2 tethered EGF on the
film surface.
[0117] Cell Culture
[0118] PC12 cells were seeded (medium: RMPI 1640 with 5% FBS, 10%
horse serum heat-inactivated donor herd, and supplemented with
pennicilin-streptomycin) on surfaces and cultured 3 days. To keep
the PC12 cells attached to the surface in this experiment, prior to
culturing, surfaces were exposed to 0.5 mg/ml rat tail collagen
solutions overnight at 5.degree. C. PC12s are an adrenyl tumor cell
type which differentiates into a neuronal phenotype under certain
conditions. This differentiation is similar to that of neuronal
cells in general, morphologically characterized by the formation
and extension of neurites. PC12 cells cultured in the presence of
soluble EGF are reported to undergo a morphological change induced
by the growth factor signal- cells round up on adhesive surfaces,
likely due either to down-regulation of integrins or changes in
integrin-ECM affinity induced by EGF signals. The EGF-bearing comb
films are non-adhesive to cells and the collagen treatment leads to
only weakly cell-adhesive surfaces. PC12 cells were cultured as
described for several weeks. At 3 days initial evidence of
differentiation was observed, which became very clear after two
weeks. No differentiation was observed on controls
Example 5: Preparation of Surfaces Presenting Multiple Ligand
Types
[0119] Non-cell binding comb copolymers with PMMA backbones and
methoxy- or hydroxyl-terminated PEO side chains were prepared as
described in Example 3. The combs were subsequently used to create
substrates which present co-tethered epidermal growth factor (EGF)
and RGD. First, RGD-bearing combs were prepared in the manner
described in Example 3. The RGD-bearing combs were solvated in THF
along with non-cell binding combs activated with tresyl chloride,
and films were cast onto cleaned glass substrates using standard
spin-coating procedures. Films were dried under vacuum for 24 hrs
to remove remaining solvent. The substrate was then exposed to an
EGF solution, enabling the covalent attachment of EGF to the
activated comb side chains at the surface through the terminal
amine group of the EGF. Solutions of 10 ng/mL EGF in PBS were
incubated on surfaces containing the activated combs mixed with the
RGD combs, or with controls containing unactivated combs. The
amount of EGF covalently linked to the substrates under these
conditions was 8.5.+-.1.5 ng/cm.sup.2. For comparison, maximum DNA
synthesis response in primary rat hepatocytes cultured on tethered
EGF occurred at a density of less than 1 ng/cm.sup.2 (1000 EGF
molecules/.mu.m.sup.2) and the approximate density of receptors on
the cell surface of hepatocytes or WT NR6 is 100-400
molecules/.mu.m.sup.2 Thus the amount of EGF which can be
covalently linked on the RGD-bearing substrate is sufficient for
influencing cell response. Further, the amount of non-specifically
adsorbed EGF on the comb surfaces, 0.9.+-.0.3 ng/cm.sup.2, is
negligible relative to the amount that is covalently coupled. WT
NR6 fiberblasts were cultered for 24 hours as previously described
on the EGF/RGD substrates. Cells were observed to adhere and spread
on the mixed ligand surface.
Example 6. Comb Copolymer-Stabilized Latexes
[0120] Comb Synthesis
[0121] Comb polymer stabilizer was synthesized free-radically in
solution Methyl methacrylate (MMA), methoxy poly(ethylene glycol)
methacrylate (MPEGMA), and poly(ethylene glycol) methacrylate
(PEGMA) were added to benzene in equal weight fractions of the two
PEG macro-monomers, for a total monomer concentration of 0.6M.
Azo(bis)iso-butyronitrile was added at a molar ratio of 20:1
[monomer]:[initiator]. The solution was degassed under nitrogen 15
minutes, followed by polymerization at 60.degree. C. for 16 hours.
The comb polymer was purified by repeated precipitation in
petroleum ether. In order to obtain latex beads with
protein-resistant surfaces, the ratio of PEGMA/MPEGMA units to MMA
units in the comb stabilizer copolymers was first optimized.
Initial studies found that combs containing 40 wt % of the
PEGMA/PEGMA units formed films that were cell resistant in the
presence of serum and simultaneously resistant to dissolution in
water-based media. Combs of this composition were soluble in 50/50
water/ethanol, and thus served as an ideal stabilizer for
preparation of the polymer latexes. Combs with greater PEG
fractions (.about.50 wt % or more) were water soluble over time.
The comb stabilizer had a total molecular weight, prior to peptide
attachment, of approximately 23,000 Daltons.
[0122] To obtain adhesion ligand-bearing latexes, RGD-bearing combs
were first prepared by solution coupling GRGDSP (Gibco) to the ends
of the PEGMA units of the comb. Coupling was accomplished through
the reaction of tresyl chloride-activated combs and the N-terminal
amine of the peptide. The hydroxyl ends of the PEGMA units of the
comb were activated by reaction with 2,2,2-trifluoroethanesulfonyl
chloride (tresyl chloride) in tetrahydrofuran. The comb copolymer
(150 mg) was dissolved in 25 ml dry THF at 5.degree. C.
Triethylamine (200 .mu.l) and tresyl chloride (250 .mu.l ) were
added and the reaction was allowed to proceed 3 hours. The
activated polymer was then recovered by filtration and
precipitation in petroleum ether. GRGDSP peptide was coupled to the
activated polymer by adding 150 .mu.l GRGSP solution (1 mg/ml in pH
7.4 phosphate buffered saline) to 2.5 ml of activated comb solution
(0.02 g/ml in THF) at 5.degree. C. and stirring for 3 hours. The
RGD-coupled comb was recovered by overnight precipitation in
deionized water. Amounts of peptide coupled were determined by a
colorimetric assay (microBCA, Pierce Chemical Co.). RGD content was
found to be 0.3 wt % (1 RGD peptide per .about.10 comb polymer
molecules).
[0123] Acrylic Latex Syntheses
[0124] Methacrylate- and acrylate-based polymer latexes were
synthesized by dispersion polymerization employing the comb
polymers as stabilizing agents. Latexes of four different
compositions were prepared in this study: pure poly(methyl
methacrylate), poly(methyl methacrylate-co-butyl acrylate),
poly(ethyl methacrylate-co-methyl acrylate), and poly(ethyl
methacrylate-co-butyl methacrylate). In addition, one
cell-interactive poly(methyl methacrylate) latex was prepared using
the RGD-comb stabilizer. Comb stabilizer was dissolved in a 1:1
mixture by volume of ethanol and water, followed by addition of
methacrylate/acrylate monomers and 0.57 g ammonium persulfate.
Reactions were allowed to proceed 18 hours at 60.degree. C. with
stirring. Reactions began as one phase, clear solutions, and became
opaque white dispersions during polymerization. After completion of
the syntheses, all latexes were purified by repeated centrifugation
and redispersion in water/ethanol. Suspensions were stable over
greater than 24 hour periods and could be resuspended after
extended storage via ultrasonic mixing. All latexes were
ultrasonically treated for at least 30 minutes prior to use.
Molecular weights of the polymers comprising the latex particles
ranged from approximately 400,000 Daltons to 1 millon Daltons.
Glass transition temperatures of the particles ranged from
-26.degree. C. to 105.degree. C., depending on the monomer
constistuents used in the polymerization.
[0125] Morphology of the latex beads was assessed by examining
beads cast on substrates using a JEOL 6320 field emission scanning
electron microscope operating at a 4.0 kV accelerating voltage.
Samples were shadowed with gold prior to imaging. Average particle
diameters were measured from SEM micrographs, with at least 300
particles measured for each sample. Average particle sizes ranged
from 0.2 to 1.8 micrometers. All of the latexes had size
polydispersities below 1.06. In each case, the comb stabilizer
comprised below 1 wt % of the total latex bead composition.
[0126] Latex Film Preparation and Characterization
[0127] Films were prepared from the latex suspensions by
spin-coating the particles (0.02-0.03 g/ml in water/ethanol) at
1000 rpm onto cleaned glass substrates. To form contiguous films
from the cast particles, short heat treatments (30-60 seconds) were
applied to the samples by a heat gun set at 800-900.degree. C.
Coalescence of the particles was confirmed by examining the
surfaces in a light microscope. For cell culture and contact angle
experiments, poly(methyl methacrylate) homopolymer (not a latex)
served as a control substrate. PMMA (Polysciences, 68K g/mole,
M.sub.W/M.sub.N=1.07) films were spincoated from a 0.03 g/ml
toluene solution onto clean glass coverslips at 1000 rpm, followed
by drying in vacuo at 70.degree. C. 24 hours.
[0128] Contact angles of water on coalesced latex film surfaces,
films of the non-cell binding comb, and on the PMMA control film
were measured using a VCA2000 video contact angle system (AST
Inc.). Advancing/receding contact angles were measured by capturing
digital images of deionized water droplets placed by syringe on
virgin surfaces and measuring angles from the images. In all cases
but the control, the advancing contact angles are seen to be
relatively constant and independent of drop volume, while the
receding angles show significant changes in contact angle with drop
size. All of the latex films showed hysteresis of 25.degree. or
more in these measurements, while pure PMMA displayed only a
.about.10.degree. change. Though contact angle hysteresis can occur
for a number of reasons, the likeliest explanation for the contact
angle hysteresis observed here is the reorganization/hydration of
the PEG side chains at the surface of the films upon wetting. That
the comb copolymer is not water-soluble was confirmed by
ellipsometry measurements of dried latex and comb film thicknesses
before and after water immersion, which showed no detectable loss
of polymer. These results provide a strong indication that the comb
stabilizer remains at the surface once the latex particles coalesce
into a homogeneous film.
[0129] Cell Culture
[0130] All cell culture reagents were purchased from Gibco. NR6
fibroblasts transfected with the wild-type human epidermal growth
factor receptor (WT NR6) were cultured in modified Eagle's medium
alpha (MEM-.alpha.) supplemented with 7.5% fetal bovine serum,
L-glutamine, non-essential amino acids, sodium pyruvate,
penicillin-streptomycin, and gentamycin ntibiotic.
[0131] Cell attachment studies were performed by seeding 20,000
cells/cm.sup.2 onto the non-cell binding comb copolymer films,
coalesced PMMA latex films, and two controls: tissue culture
polystyrene (TCPS) and pure PMMA films. Cell were seeded in 1.5 ml
serum-containing growth medium for 24 hours, followed by aspiration
to remove unattached cells and application of fresh medium.
Morphology/adhesion of cells to latex films was then assessed using
a Zeiss Axiovert 100 phase contrast microscope.
[0132] After 24 hours, cells are attached and spread on both
controls, presumably via protein layers adsorbed onto these
surfaces. However, the PEG side chains of the comb copolymer
stabilizer provide complete cell resistance for the comb film under
these stringent conditions. Likewise, the PMMA latex film presents
a surface with essentially equivalent cell-resistant capacity,
although the comb stabilizer comprises only .about.1 wt % of the
total polymer film. This observation is further evidence that the
combs remain localized to the film surface during coalescence.
[0133] Films coalesced from the RGD-bearing PMMA latex were
prepared and seeded with WT NR6 cells as before. In contrast to
latexes stabilized with the non-cell binding combs, coalesced films
of the RGD-bearing latex elicited cell attachment and spreading.
Apparently, surface densities of RGD ligand obtained for these
latex films are comparable to the pure RGD-linked comb, although
the latex film contains {fraction (1/100)} as much total peptide.
Specificity of the adhesion of cells to the RGD-bearing surface was
confirmed by adding excess soluble GRGDSP (45 mM) to the culture
media. All cells were observed to detach within 1 hour of soluble
RGD administration.
[0134] Modifications and variations of the present invention will
be obvious to those skilled in the art from the foregoing detailed
description. Such modifications and variations are intended to come
within the scope of the following claims.
* * * * *