U.S. patent number RE46,707 [Application Number 14/604,262] was granted by the patent office on 2018-02-13 for variants of c-type natriuretic peptide.
This patent grant is currently assigned to BioMarin Pharmaceutical Inc.. The grantee listed for this patent is BioMarin Pharmaceutical Inc.. Invention is credited to Mika Aoyagi-Scharber, Sianna Castillo, Shinong Long, Augustus O. Okhamafe, Christopher P. Price, Michel Claude Vellard, Daniel J. Wendt.
United States Patent |
RE46,707 |
Wendt , et al. |
February 13, 2018 |
Variants of C-type natriuretic peptide
Abstract
The present disclosure provides variants of C-type natriuretic
peptide (CNP), pharmaceutical compositions comprising CNP variants,
and methods of making CNP variants. The CNP variants are useful as
therapeutic agents for the treatment of diseases responsive to CNP,
including but not limited to bone-related disorders, such as
skeletal dysplasias (e.g., achondroplasia), and vascular smooth
muscle disorders (e.g., restenosis and arteriosclerosis).
Inventors: |
Wendt; Daniel J. (Walnut Creek,
CA), Long; Shinong (San Ramon, CA), Castillo; Sianna
(Novato, CA), Price; Christopher P. (Kentfield, CA),
Aoyagi-Scharber; Mika (Mill Valley, CA), Vellard; Michel
Claude (San Rafael, CA), Okhamafe; Augustus O. (Concord,
CA) |
Applicant: |
Name |
City |
State |
Country |
Type |
BioMarin Pharmaceutical Inc. |
Novato |
CA |
US |
|
|
Assignee: |
BioMarin Pharmaceutical Inc.
(Novato, CA)
|
Family
ID: |
43124672 |
Appl.
No.: |
14/604,262 |
Filed: |
January 23, 2015 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
Issue Date |
|
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12784117 |
May 20, 2010 |
8198242 |
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61180112 |
May 20, 2009 |
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61254563 |
Oct 23, 2009 |
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Reissue of: |
13466672 |
May 8, 2012 |
8598121 |
Dec 3, 2013 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61K
47/60 (20170801); A61P 19/00 (20180101); C07K
14/58 (20130101); A61P 9/00 (20180101); A61K
38/22 (20130101); A61K 47/26 (20130101); A61P
13/12 (20180101); A61P 7/10 (20180101); A61P
9/04 (20180101); A61P 19/08 (20180101); A61P
9/12 (20180101); A61K 47/20 (20130101); A61P
9/10 (20180101); A61K 9/08 (20130101); A61K
47/22 (20130101); A61K 38/2242 (20130101); A61K
47/10 (20130101); A61K 47/48215 (20130101); C07K
14/58 (20130101); A61P 21/00 (20180101); A61K
38/00 (20130101); G01N 2800/52 (20130101); A61K
38/00 (20130101); G01N 2800/52 (20130101); G01N
2800/105 (20130101); G01N 2800/385 (20130101); G01N
2333/58 (20130101); G01N 2800/10 (20130101); G01N
2800/10 (20130101); G01N 2800/385 (20130101); G01N
2333/58 (20130101); G01N 2800/105 (20130101) |
Current International
Class: |
A61K
38/22 (20060101); A61K 38/16 (20060101); A61K
38/00 (20060101); C12P 21/04 (20060101); C07K
14/58 (20060101) |
Field of
Search: |
;514/12.4 |
References Cited
[Referenced By]
U.S. Patent Documents
Foreign Patent Documents
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0466174 |
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Jan 1992 |
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EP |
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0497368 |
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Aug 1992 |
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EP |
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1743653 |
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Jan 2007 |
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EP |
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WO-94/20534 |
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Sep 1994 |
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WO |
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WO-00/61631 |
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Oct 2000 |
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WO |
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WO-02/074234 |
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Sep 2002 |
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WO |
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WO-04/047871 |
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Jun 2004 |
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WO |
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WO-09/67639 |
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May 2009 |
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WO |
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|
Primary Examiner: Railey; Johnny F
Attorney, Agent or Firm: Hunter-Ensor; Melissa Serunian;
Leslie Greenberg Traurig, LLP
Parent Case Text
CROSS-REFERENCE TO RELATED APPLICATIONS
The present application claims the priority and benefit of U.S.
Provisional Application No. 61/254,563, filed on Oct. 23, 2009, and
U.S. Provisional Application No. 61/180,112, filed on May 20, 2009,
the disclosure of each of which is incorporated herein by reference
in its entirety.
Claims
What is claimed is:
.[.1. A variant of C-type natriuretic peptide (CNP) selected from
the group consisting of: TABLE-US-00045 (SEQ ID NO: 179)
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Gly-CNP53); (SEQ ID NO: 185)
PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Pro-CNP53); (SEQ ID NO: 190)
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC (Met-CNP53)
(SEQ ID NO: 180) DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNS
GLGC [CNP-53(M48N)].
.].
2. A pharmaceutical composition comprising a CNP variant of claim
.[.1.]. .Iadd.15.Iaddend., and a pharmaceutically acceptable
excipient, carrier or diluent.
3. The composition of claim 2, which is a lyophilized formulation
prepared from a formulation that comprises a citric acid/citrate
buffer or an acetic acid/acetate buffer having a pH from about 4 to
about 6.
4. The composition of claim 3, wherein the lyophilized formulation
is prepared from a formulation that further comprises (a) an
isotonicity-adjusting agent or a bulking agent or (b) an
antioxidant.
5. A method of treating a bone-related disorder or skeletal
dysplasia, comprising administering a CNP variant to a subject in
need thereof, wherein the CNP variant is a CNP variant according to
claim .[.1.]. .Iadd.15.Iaddend., and wherein the bone-related
disorder or skeletal dysplasia is selected from the group
consisting of achondroplasia, hypochondroplasia, short stature,
dwarfism and homozygous achondroplasia.
.[.6. A method for recombinant production of a CNP variant,
comprising culturing in a medium a host cell comprising a first
polynucleotide encoding a CNP variant polypeptide linked to a
second polynucleotide encoding a cleavable peptide or protein under
conditions that result in expression of a fusion polypeptide
encoded by the polynucleotides, wherein the fusion polypeptide
comprises the CNP variant polypeptide directly linked to the
cleavable peptide or protein or indirectly linked thereto via a
linker, wherein the CNP variant is selected from the group
consisting of: TABLE-US-00046 (SEQ ID NO: 179)
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Gly-CNP53); (SEQ ID NO: 185)
PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Pro-CNP53); (SEQ ID NO: 190)
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Met-CNP53); (SEQ ID NO: 180)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNS GLGC
[CNP-53(M48N)].
.].
7. The method of claim .[.6.]. .Iadd.16.Iaddend., wherein the
cleavable peptide or protein is selected from the group consisting
of histidine tags, human transcription factor TAF12, TAF12
fragments, TAF12 histone fold domain, mutants of TAF12 and
fragments thereof, TAF12(C/A), TAF12(D/E), TAF12(4D/4E),
TAF12(6D/6E), TAF12(10D/10E), TAF12(C/A & D/E), TAF12(C/A &
4D/4E), TAF12(C/A & 6D/6E), TAF12(C/A & 10D/10E),
ketosteroid isomerase, maltose-binding protein,
.beta.-galactosidase, glutathione-S-transferase, thioredoxin,
chitin-binding domain, BMP-2, BMP-2 mutants, BMP-2(C/A), and
mutants and fragments thereof.
8. The method of claim .[.6.]. .Iadd.16.Iaddend., wherein the host
cell is bacterial.
9. The method of claim .[.6.]. .Iadd.16.Iaddend., wherein the
fusion polypeptide is expressed as a soluble protein or as an
inclusion body.
10. The method of claim .[.6.]. .Iadd.16.Iaddend., further
comprising isolating the expressed fusion polypeptide from the host
cell or culture medium.
11. The method of claim 10, further comprising contacting the
isolated fusion polypeptide with a cleaving agent selected from the
group consisting of formic acid, cyanogen bromide (CNBr),
hydroxylamine, protein self cleavage, Factor Xa, enterokinase,
ProTEV, and SUMO protease.
.[.12. A CNP variant produced according to the method of claim 6,
wherein the CNP variant is selected from the group consisting of:
TABLE-US-00047 (SEQ ID NO: 179)
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Gly-CNP53)); (SEQ ID NO: 185)
PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Pro-CNP53)); (SEQ ID NO: 190)
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Met-CNP53)); (SEQ ID NO: 180)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSG LGC
[CNP-53(M48N)]).
.].
13. A method of increasing long bone growth, comprising
administering a CNP variant according to claim .[.1.]. .Iadd.15
.Iaddend.to a subject in need thereof, where the administration
increases long bone growth.
14. A CNP variant according to claim .[.1.]. .Iadd.15
.Iaddend.useful for increasing long bone growth or treating
achondroplasia, hypochondroplasia, short stature, dwarfism, or
homozygous achondroplasia in a subject in need thereof.
.Iadd.15. A variant of C-type natriuretic peptide (CNP) selected
from the group consisting of: TABLE-US-00048
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
179) (Gly-CNP53); PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM
SGLGC (SEQ ID NO: 185) (Pro-CNP53);
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
190) (Met-CNP53); DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNS
GLGC (SEQ ID NO: 180) [CNP-53(M48N)];
a CNP53 variant comprising a K4R mutation in the CNP22 portion of
the peptide; a CNP53 variant comprising a furin cleavage site
mutation; TABLE-US-00049
PEG-GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDR IGSMSGLGC (SEQ ID
NO: 179) (PEG-Gly-CNP53);
PEG-PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDR IGSMSGLGC (SEQ ID
NO: 185) (PEG-Pro-CNP53);
PEG-MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDR IGSMSGLGC (SEQ ID
NO: 190) (PEG-Met-CNP53); and
PEG-DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRI GSNSGLGC (SEQ ID
NO: 180) [PEG-CNP-53(M48N)].
.Iaddend.
.Iadd.16. A method for recombinant production of a CNP variant,
comprising culturing in a medium a host cell comprising a first
polynucleotide encoding a CNP variant polypeptide linked to a
second polynucleotide encoding a cleavable peptide or protein under
conditions that result in expression of a fusion polypeptide
encoded by the polynucleotides, wherein the fusion polypeptide
comprises the CNP variant polypeptide directly linked to the
cleavable peptide or protein or indirectly linked thereto via a
linker, wherein the CNP variant is selected from the group
consisting of: TABLE-US-00050
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
179) (Gly-CNP53); PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM
SGLGC (SEQ ID NO: 185) (Pro-CNP53);
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
190) (Met-CNP53); DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNS
GLGC (SEQ ID NO: 180) [CNP-53(M48N)];
a CNP53 variant comprising a K4R mutation in the CNP22 portion of
the peptide; and a CNP53 variant comprising a furin cleavage site
mutation..Iaddend.
.Iadd.17. A CNP variant produced according to the method of claim
16, wherein the CNP variant is selected from the group consisting
of: TABLE-US-00051
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
179) (Gly-CNP53); PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM
SGLGC (SEQ ID NO: 185) (Pro-CNP53);
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSM SGLGC (SEQ ID NO:
190) (Met-CNP53); DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNS
GLGC (SEQ ID NO: 180) [CNP-53(M48N)];
a CNP53 variant comprising a K4R mutation in the CNP22 portion of
the peptide; and a CNP53 variant comprising a furin cleavage site
mutation..Iaddend.
Description
FIELD OF THE DISCLOSURE
The field of the disclosure relates, in general, to variants of
C-type natriuretic peptide (CNP), compositions comprising CNP
variants, methods of making CNP variants, and methods of using CNP
variants to treat disorders responsive to CNP, including but not
limited to bone-related disorders such as skeletal dysplasias
(e.g., achondroplasia) and vascular smooth muscle disorders.
BACKGROUND OF THE DISCLOSURE
The natriuretic peptide family consists of three structurally
related peptides: atrial natriuretic peptide (ANP) (Genbank
Accession No. NP_006163, for the ANP precursor protein, NPPA),
brain natriuretic peptide (BNP) (GenBank Accession No. NP_002512,
for the BNP precursor protein, NPPB), and C-type natriuretic
peptide (CNP) (Biochem. Biophys. Res. Commun., 168: 863-870 (1990)
(GenBank Accession No. NP_077720, for the CNP precursor protein,
NPPC) (J. Hypertens., 10: 907-912 (1992)). These small, single
chain peptides (ANP, BNP, CNP) have a 17-amino acid loop structure
(Levin et al., N. Engl. J. Med., 339: 863-870 (1998)) and have
important roles in multiple biological processes. ANP and BNP bind
to and activate the natriuretic peptide receptor A (NPR-A), also
termed guanalyl cyclase A (GC-A), resulting in higher intracellular
cyclic guanosine monophosphate (cGMP) levels. Likewise, CNP
interacts with NPR-B (GC-B) to stimulate the generation of cGMP (J.
Hypertens., 10: 1111-1114 (1992)). A third type of receptor, NPR-C,
binds each of the natriuretic peptides with high affinity and
functions primarily to capture the peptides from the extracellular
compartment and deposit the peptides into lysosomes, where they are
degraded (Science, 238: 675-678 (1987)). ANP and BNP are produced
primarily within the muscle cells of the heart, and are believed to
have important roles in cardiovascular homeostasis (Science, 252:
120-123 (1991)). CNP is expressed more widely, including in the
central nervous system, reproductive tract, bone and endothelium of
blood vessels (Hypertension, 49: 419-426 (2007)).
In humans, CNP is initially produced from the natriuretic peptide
precursor C(NPPC) gene as a single chain 126-amino acid pre-pro
polypeptide (Biochem. Biophys. Res. Commun., 168: 863-870 (1990)).
Removal of the signal peptide yields pro-CNP, and further cleavage
by the endoprotease furin generates an active 53-amino acid peptide
(CNP-53), which is secreted and cleaved again by an unknown enzyme
to produce the mature 22-amino acid peptide (CNP-22) (Wu, J. Biol.
Chem. 278: 25847-852 (2003)). CNP-53 and CNP-22 differ in their
distribution, with CNP-53 predominating in tissues, while CNP-22 is
mainly found in plasma and cerebrospinal fluid (J. Alfonzo, Recept.
Signal. Transduct. Res., 26: 269-297 (2006)). The predominant CNP
form in cartilage is unknown. Both CNP-53 and CNP-22 bind similarly
to NPR-B. Furthermore, they both induce cGMP production in a
dose-dependent and similar fashion (V T Yeung, Peptides, 17:
101-106 (1996)).
Natural CNP gene and polypeptide have been previously described.
U.S. Pat. No. 5,352,770 discloses isolated and purified CNP-22 from
porcine brain identical in sequence to human CNP and its use in
treating cardiovascular indications. U.S. Pat. No. 6,034,231
discloses the human gene and polypeptide of proCNP (126 amino
acids) and the human CNP-53 gene and polypeptide.
Clearance of CNP from the extracellular space occurs through the
action of membrane-bound neutral endopeptidase (NEP), which rapidly
degrades CNP (Biochem. J., 291 (Pt 1): 83-88 (1993)), and through
NPR-C, which binds to and deposits CNP into lysosomes, where CNP is
degraded. CNP has been shown to have an in vivo half-life of 2.6
min in the normal human (J. Clin. Endocrinol. Metab., 78: 1428-35
(1994)). The low plasma concentration of CNP (J. Bone Moner. Res.,
19 (Suppl. 1) S20 (2004)) and its co-expression with NPR-B in a
number of tissues suggests that CNP functions primarily through an
autocrine/paracrine mechanism.
As stated above, CNP binds to and activates natriuretic peptide
receptor B (NPR-B), also termed guanylyl cyclase B (GC-B),
resulting in higher intracellular cyclic guanosine monophosphate
(cGMP) levels. Downstream signaling mediated by cGMP generation
influences a diverse array of biological processes that include
endochondral ossification. Accordingly, elevated or depressed
levels of any of the components in this pathway may lead to
aberrant bone growth. For example, knockout of either CNP or NPR-B
in mouse models results in animals having a dwarfed phenotype with
shorter long bones and vertebrae. Mutations in human NPR-B that
block proper CNP signaling have been identified and result in
dwarfism (Olney, et al., J. Clin. Endocrinol. Metab. 91(4):
1229-1232 (2006); Bartels, et al., Am. J. Hum. Genet. 75: 27-34
(2004)). In contrast, mice engineered to produce elevated levels of
CNP display elongated long bones and vertebrae.
Achondroplasia is a result of an autosomal dominant mutation in the
gene for fibroblast growth factor receptor 3 (FGFR-3), which causes
an abnormality of cartilage formation. FGFR-3 normally has a
negative regulatory effect on chondrocyte growth, and hence bone
growth. In achondroplasia, the mutated form of FGFR-3 is
constitutively active, which leads to severely shortened bones.
Both chondrocyte proliferation and differentiation appear to be
disturbed, leading to remarkably short growth plate cartilage (P.
Krejci et al., J. Cell Sci. 118: 5089-5100 (2005)). Endochondral
ossification is the process that governs longitudinal long-bone
growth. There are four zones of the growth plate--resting,
proliferative, hypertrophic and zone of calcification. In the
growth plate, NPR-B is expressed by proliferative cells while NPR-C
is expressed by hypertrophic cells (Yamashite et al., J. Biochem.
127: 177-179 (2000)). In normal endochondral bone growth,
chondrocytes organize in columns and proliferate in the
proliferative zone of the growth plate. These columns are
disorganized in achondroplasia patients. Additionally, the
hypertrophic zone is where the cells become large and eventually
apoptose (lyse), leading to osteocyte invasion and mineralization.
The hypertrophic chondrocytes and the overall size of the zone are
much smaller in achondroplasia patients than in normal patients.
CNP is an agonist for NPR-B, a positive regulator of chondrocyte
and bone growth. Downstream signaling of CNP/NPR-B inhibits the
FGFR-3 pathway at the level of mitogen-activated protein kinase
(MAP K). Inhibition at MAP K promotes proliferation and
differentiation of the chondrocytes in the proliferative and
hypertrophic zones of the growth plate, resulting in bone
growth.
In humans activating mutations of FGFR-3 are the primary cause of
genetic dwarfism. Mice having activated FGFR-3 serve as a model of
achondroplasia, the most common form of the skeletal dysplasias,
and overexpression of CNP rescues these animals from dwarfism.
Accordingly, CNP and functional variants of CNP are potential
therapeutics for treatment of the various skeletal dysplasias.
Therapeutic use of CNP is currently limited by its short plasma
half-life, which has been shown to be 2.6 minutes in vivo in humans
(J. Clin. Endocrinol. Metab., 78: 1428-35 (1994)). To increase CNP
concentration above intrinsic levels (about 5 pM) typically found
in human plasma, continuous infusion has been necessary in all
human and animal studies using systemically administered CNP. A CNP
variant having a longer in vivo serum half-life and exhibiting
similar or improved activity to that of wild-type CNP is important
for a sustainable therapeutic strategy. Two mechanisms by which the
half-life of CNP is reduced in human plasma are degradation by
neutral endopeptidase (NEP) and clearance by natriuretic peptide
receptor C(NPR-C) (Growth Horm. & IGF Res., 16: S6-S14 (2006)).
Modifications of peptides reportedly can improve resistance to
endopeptidase and exopeptidase cleavage (Amino Acids, 30: 351-367
(2006); Curr. Opin. Biotech., 17: 638-642 (2006)).
The biological activities of various analogs and derivatives of CNP
have been evaluated. By substituting S-methyl Cys in place of both
Cys.sub.6 and Cys.sub.22, cyclization of the peptide via a
Cys6-Cys22 disulfide linkage was reportedly shown to be important
for the activity of CNP in stimulating cGMP formation (Biochem.
Biophys. Res. Comm., 183: 964-969 (1992), also using alanine
scanning to identify amino acids important for CNP functionality).
A significant additional enhancement of activity reportedly results
from the combined presence of the amino acids Leu.sub.9,
Lys.sub.10, and Leu.sub.11. U.S. Pat. No. 5,434,133 describes CNP
analogs comprising CNP-22 with substitutions at amino acid position
6, 7, 9, 11, or 22, wherein the amino acid is selected from Cys or
Pmp (penta-cyclomercaptopropionic acid) at position 6, Phe,
4-chloro-Phe, 4-fluoro-Phe, 4-nitro-Phe, or Cha (3-cyclohexyl-Ala)
at position 7, Gly, Val, Aib, or tLeu at position 9, Leu or Ile at
position 11, and Cys or Pmp at position 22.
U.S. Patent Publication No. 2004/0138134 (now U.S. Pat. No.
7,276,481) describes CNP variants comprising amino acids Cys.sub.6
to Cys.sub.22 of CNP-22 ("CNP-17") which include at least one
substitution for another natural amino acid at position 9, 10, 11,
16, 17, 19, or 20, CNP variants with insertions and deletions, such
as addition of a His residue at the reported primary site of NEP
cleavage, between Cys.sub.6 and Phe.sub.7, and methods of using
such variants for increasing the size of a bone growth plate in
abnormal bone and elongation of an abnormal bone. However, no
significant gains in activity as measured by cGMP production were
obtained for these variants, and activity was diminished for nearly
all of the variants, as observed in an in vitro cell-based method
(Example 7). Further no supportive data, such as for example in
vitro stability or in vivo determination of improved
pharmacokinetics (PK) were provided to substantiate the asserted
NEP resistance and NPR-C resistance of the CNP analogs. U.S. Pat.
No. 6,743,425 discloses substances for treating achondroplasia
which activate NPR-B/GC-B and are peptides or low molecular weight
compounds, including the C-type natriuretic peptides CNP-22 and
CNP-53. PCT Publication No. WO 94/20534 discloses a chimera of
CNP-22 and the 5-amino acid C-terminus of ANP designated as the
vasonatrin peptide (VNP), a limited number of amino acid
substitutions and cyclic chimeric peptides that result from
formation of a disulfide or double bond.
Approaches for improving the half-life of other natriuretic peptide
family members include decreasing the affinity of ANP for NPR-C
(U.S. Pat. No. 5,846,932), utilizing pentapeptide antagonists of
NPR-C (WO 00/61631), and co-administering NEP inhibitors such as
thiorphan and candoxatril (Clin. Exp. Pharma. Physiol., 25: 986-991
(1997), Hyperten., 30: 184-190 (1997)). WO 2004/047871 describes
conjugates of BNP and BNP variants to polyalkylene glycol moieties,
sugar moieties, polysorbate moieties, polycationic moieties, and
other hydrophilic polymer moieties that reportedly exhibit improved
half-life in circulation and reportedly are useful for the
treatment of acute congestive heart failure.
There have been no published reports, however, on a successful
strategy for making CNP resistant to NEP while retaining its
functionality.
SUMMARY OF THE DISCLOSURE
The present disclosure relates to variants of C-type natriuretic
peptide (CNP) which are useful in the treatment of bone-related
disorders (e.g., achondroplasia) and vascular smooth muscle
disorders. The disclosure encompasses CNP variants having increased
serum half-life, e.g. as a result of reduced ability to bind to
neutral endopeptidase (NEP), greater resistance to proteolysis by
NEP and/or reduced affinity to the clearance natriuretic peptide
receptor C(NPR-C), while retaining the functionality of CNP.
The wild-type sequence of human CNP-22 (referred to herein as
"hCNP22", "wtCNP22" or "CNP22") is set forth below:
TABLE-US-00001 (SEQ ID NO: 1) (N-terminus)
Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5-Cys.sub.6-Phe.sub.7-
Gly.sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub.12-Arg.sub.13-Ile.sub.1-
4-Gly.sub.15-
Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub.19-Leu.sub.20-Gly.sub.21-Cys.sub-
.22.
Positions 6 to 22 of CNP22 form a cyclic domain by means of a
disulfide bond between Cys6 and Cys22. The 17-amino acid cyclic
structure has been shown to be important for binding of CNP to
NPR-B (Schiller, Biochem. Biophys. Res. Commun., 138: 880-886
(1986)). The amino acid sequence of positions 6 to 22 of CNP22 is
referred to herein as "CNP17" (SEQ ID NO: 2).
CNP is susceptible to NEP cleavage at a number of sites: Cys6-Phe7,
Gly8-Leu9, Lys10-Leu11, Arg13-Ile14, Ser16-Met17 and Gly19-Leu20.
In one embodiment, the disclosure encompasses a CNP variant that is
(1) modified to increase its overall size or molecular weight,
e.g., to a range from about 2.6 kDa or 2.8 kDa to about 4 kDa, 4.2
kDa, 4.4 kDa, 4.6 kDa, 4.8 kDa, 5 kDa, 5.2 kDa, 5.4 kDa, 5.6 kDa,
5.8 kDa, 6 kDa, 6.2 kDa, 6.4 kDa, or to about 7 kDa, 7.2 kDa or
about 8.2 kDa, and/or (2) modified at certain amino acid positions
to reduce its susceptibility to NEP cleavage at 1, 2, 3, 4, 5 or
all 6 of the sites listed above. The size or molecular weight of
the CNP variant can be increased by various means, e.g., by
conjugating additional amino acids and/or other kinds of chemical
(e.g., natural or synthetic polymeric) groups to the peptide
sequence at, e.g., the N-terminus, the C-terminus and/or side
chain(s), and/or by using natural amino acids, unnatural amino
acids, and/or peptidomimetics with bulkier side chains. The CNP
variant is optionally further conjugated to other functional or
structural moieties. Optionally in combination with any of the
embodiments described herein, mutation(s) (e.g., substitution(s),
addition(s), and/or deletion(s)) may be introduced to certain
position(s) of CNP22 to reduce the CNP variants' affinity to NPR-C.
Further modifications may be made without affecting NEP resistance
or CNP activity, e.g., conservative substitutions, or other
modifications known in the art.
In one embodiment, the CNP variant is represented by the general
formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5-Cys.sub.6-Phe.sub.7-
-Gly.sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub.12-Arg.sub.13-Ile.sub.1-
4-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub.19-Leu.sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 5), wherein:
the CNP variant comprises one or more modified amino acids, which
may result in modified peptide bonds (e.g., through use of peptide
bond isosteres), at a position corresponding to one or more of the
following CNP residues: Gly1, Lys4, Gly5, Cys6, Phe7, Gly8, Leu9,
Lys10, Leu11, Ile14, Gly15, Ser16, Met17, Gly19, Leu20 and Gly21;
and
(x) and (z) independently may be absent or may be an amino acid
sequence derived from a natriuretic polypeptide (e.g., NPPC, ANP,
BNP) or a non-natriuretic polypeptide (e.g., human serum albumin
(HSA), IgG, etc.).
In an embodiment, the CNP variant includes: (1) a modification at
an amino acid position corresponding to one of positions 6, 7 or 8
(Cys6, Phe7 or Gly8) of CNP22, (2) optionally deletion, addition
and/or substitution of any or all of the amino acids at positions
1-5 (Gly1, Leu2, Ser3, Lys4, and Gly5) and (3) optionally up to 1,
2, 3, 4, 5, 6, 7, 8, 9 or 10 further modifications (deletions,
additions and/or substitutions) at positions corresponding to
positions 6-22, of which 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 may be
conservative substitutions or other substitutions described herein
or known in the art.
It is understood that a reference to a particular amino acid
position by number (e.g. position 7 of CNP22) refers to the
corresponding amino acid position in any CNP variant, even if the
number of the position in that CNP variant has changed due to
preceding insertions or deletions. For example, a reference to
"position 7" or "Phe7" would mean the corresponding position 2 for
a CNP variant in which the first five amino acids had been deleted.
Similarly, a reference to "position 7" would mean the corresponding
position 8 for a CNP variant in which one amino acid had been added
to the N-terminus.
In any of the embodiments described herein, the CNP variant may be
cyclized through a covalent bond between positions corresponding to
6 and 22 of CNP22. It is contemplated that the covalent bond is
formed using any methods known in the art. In another embodiment,
the CNP variant may be cyclized through a covalent bond formed
between an amino acid at or toward the N-terminus and an amino acid
at or toward the C-terminus (referred to as "terminal" amino acids
for this purpose) of the peptide. In one embodiment, the covalent
bond is formed between the side chains of the two terminal amino
acids or the amino acids at positions corresponding to 6 and 22 of
CNP22. In another embodiment, the covalent bond is formed between
the side chain of one terminal amino acid and the terminal group of
the other terminal amino acid, or between the terminal groups of
each terminal amino acid. For example, head-to-tail, side
chain-to-side chain, side chain-to-head, or side chain-to-tail
bonds are possible for the covalent bond formed between the
terminal amino acids or between the amino acids at positions
corresponding to 6 and 22 of CNP22.
In one embodiment, the disclosure provides a CNP variant having
reduced affinity to NEP, and/or greater resistance to cleavage by
NEP and/or increased in vivo serum half-life, while retaining
functionality of CNP (e.g., stimulation of cGMP production). NEP
preferably recognizes substrates smaller than about 3 kDa, due to
the limited size of its active site cavity (Oefner, J. Mol. Biol.,
296: 341-349 (2000)). In an embodiment, the CNP variants are
modified to increase their overall molecular weight to a range from
about 2.6 or 2.8 kDa to about 4, 4.6, 5, 5.2, 5.8, 6, 6.4 or 7 kDa,
e.g., by adding about 0.6 to about 5 kDa of amino acids,
hydrophilic or water-soluble polymers, hydrophobic acids (including
fatty acids), and/or carbohydrates. In specific exemplary
embodiments, the CNP variants have a molecular weight between about
2.6 kDa and about 7 kDa, or between about 2.8 kDa and 6 kDa, or
between about 2.8 kDa and about 5.8 kDa. In certain embodiments, at
least about 0.6, 0.8, 1, 1.2, 1.4, 1.6 or 1.8 kDa, or up to 2, 2.2,
2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8 or 5
kDa are added, to increase the total molecular weight of the CNP
variant, e.g., to a range from about 2.6 or 2.8 kDa up to about 4
kDa, 4.2 kDa, 4.4 kDa, 4.6 kDa, 4.8 kDa, 5 kDa, 5.2 kDa, 5.4 kDa,
5.6 kDa, 5.8 kDa, 6 kDa, 6.2 kDa, 7.2 kDa, 8.2 kDa or higher. In
some embodiments, such CNP variants comprise an amino acid sequence
at least about 70%, 75%. 80%, 85%, 90%, or 95% identical or
homologous to amino acids 6-22 of CNP22. In other embodiments, such
CNP variants comprise a substitution, insertion or deletion of 1,
2, 3, 4, 5, 6 or 7 amino acids with another natural or unnatural
amino acid or peptidomimetic. While both conservative and
non-conservative substitutions or insertions are envisioned at any
position, introduction of modifications may commence, e.g., by
conservative substitutions in regions that have been identified in
the art as involved in CNP activity or NPR-B binding, while
non-conservative substitutions may be made in those regions that
have been previously shown to be tolerant of modification.
In another embodiment, the CNP variants comprise a CNP having an
intact cyclized portion between Cys6 and Cys22, and N-terminal
and/or C-terminal tails that contain about 1-40, 1-20, 5-40, 5-35,
10-35, 15-35, 5-31, 10-31, or 15-31 amino acids and are fragments
derived from a CNP polypeptide and/or a non-CNP polypeptide. In an
embodiment, such CNP variants have a molecular weight in a range
from about 2.8 kDa to about 4, 4.6, 5, 5.2, 5.8, 6, 6.4 or 7 kDa.
Non-limiting examples of such CNP variants include wild-type CNP22
or CNP22 with one or more amino acid substitutions (e.g., a K4R
substitution), having an N-terminal and/or C-terminal extension
derived from natriuretic peptide precursor sequences (e.g., ANP,
BNP or CNP) from human or other species, a natriuretic peptide
precursor C (NPPC) variant with amino acid substitutions, additions
and/or deletions (e.g., the CNP variants may be truncations of
CNP-53 which result in peptides with a molecular weight between
about 2.8 kDa and 5.8 kDa), or other non-CNP polypeptides such as,
e.g., serum albumin or IgG protein (e.g., the CNP variants may be
CNP chimeras containing fragments of serum albumin or IgG from
human or other species).
In one embodiment, CNP variants having a total mass characterized
by the ranges described generally herein, e.g., from about 2.6 kDa
or 2.8 kDa to about 6 or 7 kDa, designed for increased resistance
to NEP degradation, are represented by the general formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(b).sub.4-Gly.sub.5-Cys.sub.6-Phe.sub.7-
-Gly.sub.8-Leu.sub.9-(h).sub.10-Leu.sub.11-Asp.sub.12-Arg.sub.13-Ile.sub.1-
4-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub.19-Leu.sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 6), wherein:
(x) is a synthetic or natural polymeric group, or a combination
thereof, wherein a non-limiting example of a synthetic polymeric
group is polyethylene glycol (PEG, also called polyethylene oxide
(PEO)), and a non-limiting example of a natural polymeric group is
an amino acid sequence containing from 1 to 35 amino acids and
derived from NPPC or variants thereof with substitutions and/or
deletions, ANP, BNP, or other non-CNP (poly)peptides such as, e.g.,
serum albumin, IgG, histidine-rich glycoproteins, fibronectin,
fibrinogen, zinc finger-containing polypeptides, osteocrin or
fibroblast growth factor 2 (FGF2);
(z) may be absent or may be a synthetic or natural polymeric group,
or a combination thereof, wherein a non-limiting example of a
synthetic polymeric group is PEG, and a non-limiting example of a
natural polymeric group is an amino acid sequence derived from a
natriuretic polypeptide (e.g., NPPC, CNP, ANP or BNP) or
non-natriuretic polypeptide (e.g., serum albumin or IgG); and
(b) and (h) independently may each be the wild type Lys at that
position or may be replaced with a conservative amino acid
substitution or any natural or unnatural amino acid or
peptidomimetic that does not have a reactive primary amine on a
side chain, including but not limited to Arg, Gly,
6-hydroxy-norleucine, citrulline (Cit), Gln, Glu or Ser. In one
embodiment, (b) is Arg. In another embodiment, for improved NEP
resistance, (b) is not Gly. In yet another embodiment, (h) is not
Arg.
Non-limiting examples of amino acid sequences derived from NPPC or
variants thereof include:
TABLE-US-00002 Arg, Glu-Arg, (SEQ ID NO: 7) Gly-Ala-Asn-Lys-Lys,
(SEQ ID NO: 8) Gly-Ala-Asn-Arg-Arg, (SEQ ID NO: 9)
Gly-Ala-Asn-Pro-Arg, (SEQ ID NO: 10) Gly-Ala-Asn-Gln-Gln, (SEQ ID
NO: 11) Gly-Ala-Asn-Ser-Ser, (SEQ ID NO: 12) Gly-Ala-Asn-Arg-Gln,
(SEQ ID NO: 13) Gly-Ala-Asn-Arg-Met, (SEQ ID NO: 14)
Gly-Ala-Asn-Arg-Thr, (SEQ ID NO: 15) Gly-Ala-Asn-Arg-Ser, (SEQ ID
NO: 16) Ala-Ala-Trp-Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn- Ala, (SEQ
ID NO: 17) Ala-Ala-Trp-Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-
Ala-Arg, (SEQ ID NO: 18)
Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp- Ala-Arg, (SEQ ID
NO: 19) Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala-
Asn-Lys-Lys, (SEQ ID NO: 20)
Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala- Asn-Arg-Arg, (SEQ
ID NO: 21) Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp-
Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-
Tyr-Lys-Gly-Ala-Asn-Lys-Lys, and (SEQ ID NO: 22)
Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp-
Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-
Tyr-Lys-Gly-Ala-Asn-Arg-Arg.
Non-limiting examples of amino acid sequences derived from non-CNP
polypeptides such as, e.g., ANP, BNP, serum albumin and IgG
include:
TABLE-US-00003 (SEQ ID NO: 23) Ser-Leu-Arg-Arg-Ser-Ser; (SEQ ID NO:
24) Asn-Ser-Phe-Arg-Tyr; (SEQ ID NO: 25)
Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly; (SEQ ID NO: 26)
Met-Val-Gln-Gly-Ser-Gly; (SEQ ID NO: 27) Lys-Val-Leu-Arg-Arg-Tyr;
(SEQ ID NO: 28) Lys-Val-Leu-Arg-Arg-His; (SEQ ID NO: 29)
Gly-Gln-His-Lys-Asp-Asp-Asn-Pro-Asn-Leu-Pro-Arg; (SEQ ID NO: 30)
Gly-Val-Pro-Gln-Val-Ser-Thr-Ser-Thr; (SEQ ID NO: 31)
Gly-Glu-Arg-Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg- Leu-Ser-Gln; and
(SEQ ID NO: 32) Gly-Gln-Pro-Arg-Glu-Pro-Gln-Val-Tyr-Thr-Leu-Pro-
Pro-Ser.
In an embodiment, the N-terminus and/or C-terminus of CNP22 or a
variant thereof independently may be conjugated to an amino acid
extension containing 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31,
32, 33, 34, 35, 36, 37, 38, 39 or 40 amino acids. In one
embodiment, the amino acid extension is derived from NPPC, CNP53,
ANP or BNP. In a specific embodiment, the amino acid extension is
Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala-Asn-Lys-Lys (SEQ ID
NO: 33). In a related embodiment, this 15-amino acid extension is
added to the N-terminus to provide a CNP variant of the formula
Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala-Asn-Lys-Lys-Gly.sub.1-Leu-
.sub.2-Ser.sub.3-(b).sub.4-Gly.sub.5-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub-
.9-(h).sub.10-Leu.sub.11-Asp.sub.12-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.s-
ub.16-Met.sub.17-Ser.sub.18-Gly.sub.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z-
) (SEQ ID NO: 34).
In one embodiment, the CNP variants comprise wtCNP22 or a variant
thereof (e.g., one having addition(s), deletion(s), and/or
substitution(s) such as, e.g., a K4R substitution) (SEQ ID NO: 35)
conjugated at the N-terminus and/or C-terminus to a hydrophilic
polymer (e.g., PEG) to increase their overall molecular size to a
range from about 2.6 kDa or 2.8 kDa to about 4, 5, 6 or 7 kDa. Such
CNP variants are optionally further conjugated at the N-terminus
and/or C-terminus to a polymeric group comprising, e.g., amino
acids, carbohydrates, hydrophobic acids and/or phospholipids, a
non-limiting example of which is an N-terminal amino acid extension
containing 1 to 35, or 5 to 31, amino acids. In an embodiment, a
hydrophilic polymeric (e.g., PEG) moiety of at least about 0.4,
0.6, 0.8, 1, 1.2, 1.4, 1.6 or 1.8 kDa, or up to about 2, 2.2, 2.4,
2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8 or 5 kDa, is
added to the N-terminus and/or C-terminus of wtCNP22 or a variant
thereof.
As shown herein, conjugation of a hydrophilic or water-soluble PEG
(or PEO) polymer of about 0.6 kDa or more to CNP22 or variants
thereof generally increases resistance to NEP cleavage markedly.
However, addition of PEG, even as small as 0.6 kDa, to wtCNP22 may
reduce CNP functionality (e.g., stimulation of cGMP signaling), and
addition of greater than about 2 or 3 kDa of PEG to CNP22 or
variants thereof may reduce CNP functional activity in a
size-dependent manner. But CNP functionality (at least comparable
to that of wtCNP22) is retained when a PEG (or PEO) polymer of
about 0.6 kDa to about 1.2 kDa, or potentially to about 2 kDa, is
conjugated to a CNP variant having an N-terminal amino acid
extension in which at least one relatively large amino acid that
may potentially be positively charged under physiological
conditions (e.g., arginine) immediately precedes the position
corresponding to Gly1 of CNP22, such as, e.g., GANRR-CNP22(K4R)
(SEQ ID NO: 36), GANPR-CNP22(K4R) (SEQ ID NO: 37), ER-CNP22 (SEQ ID
NO: 38), ER-CNP22(K4R) (SEQ ID NO: 39), R-CNP22 (SEQ ID NO: 40) and
R-CNP22(K4R) (SEQ ID NO: 41).
Accordingly, in one embodiment, PEGylated CNP variants comprise at
the N-terminus of CNP22 or a variant thereof (e.g., one having a
K4R substitution) an amino acid extension containing at least 1, 2,
3, 4 or 5 amino acids, wherein the PEG polymer is conjugated to the
N-terminus of the amino acid-extended CNP variant to result in a
total mass characterized by the ranges described generally herein,
e.g., from about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa for
increased resistance to NEP cleavage. In an embodiment, for
enhanced CNP functionality, such pegylated, amino acid-extended CNP
variants contain at least one relatively large natural or unnatural
amino acid that may potentially be positively charged under
physiological conditions, immediately preceding the position
corresponding to Gly1 of CNP22. In a specific embodiment, the
pegylated, amino acid-extended CNP variants contain at least one
arginine residue immediately preceding the position corresponding
to Gly1 of CNP22.
In addition to CNP variants conjugated at the N-terminus and/or
C-terminus to a hydrophilic or water-soluble polymer such as, e.g.,
PEG (or PEO), the disclosure encompasses CNP variants conjugated to
such a polymer at an internal site. For purposes of brevity here,
PEG (or PEO) will be used as a representative example of a
hydrophilic or water-soluble polymer. Various sites of PEGylation
of a CNP variant are possible, including but not limited to: (1)
PEGylation only at the N-terminus; (2) PEGylation only at the
C-terminus; (3) PEGylation only at an internal site (e.g., Lys4);
(4) PEGylation at both the N-terminus and the C-terminus; (5)
PEGylation at the N-terminus and an internal site; and (6)
PEGylation at the C-terminus and an internal site. For increased
resistance to NEP degradation and retention of CNP functionality,
in certain embodiments the total mass of PEGylatedCNP variants is
characterized by the ranges described generally herein, e.g., in
the range from about 2.6 kDa or 2.8 kDa to about 4, 5, 6 or 7 kDa.
In a particular embodiment, CNP17, CNP22, CNP37 (defined below) or
variants thereof (including those having amino acid additions,
substitutions and/or deletions) are PEGylated only at the
N-terminus. In another embodiment, the CNP variants are PEGylated
only at an internal site (e.g., Lys4). In yet another embodiment,
the CNP variants are PEGylated at the N-terminus and an internal
site (e.g., Lys4). In still another embodiment, for better
functionality the CNP variants are not PEGylated at a site (e.g.,
Lys10) within the cyclic domain (corresponding to Cys6 to Cys22 of
CNP22). To prevent PEGylation at an internal site, Lys4 and/or
Lys10 can be substituted with a natural or unnatural amino acid or
peptidomimetic that does not contain a reactive primary amino group
on a side chain, such as, e.g., Gly, Ser, Arg, Asn, Gln, Asp, Glu
or citrulline (Cit). In a particular embodiment, Lys4 and/or Lys10
are replaced with Arg. In another embodiment, Lys10 is not replaced
with Arg.
The disclosure contemplates use of hydrophilic or water soluble
polymers (e.g., PEG molecules) that may vary in type (e.g.,
homopolymer or copolymer; random, alternating or block copolymer;
linear or branched; monodispersed or polydispersed), linkage (e.g.,
hydrolysable or stable linkage such as, e.g., amide, imine, aminal,
alkylene, or ester bond), conjugation site (e.g., at the N-terminus
and/or C-terminus, preferably not at any of the residues in the
cyclized region of CNP (corresponding to residues 6-22 of CNP22)),
and length (e.g., from about 0.2, 0.4 or 0.6 kDa to about 2, 3, 4
or 5 kDa). The hydrophilic or water-soluble polymer can be
conjugated to the CNP peptide by means of N-hydroxy succinimide
(NHS)- or aldehyde-based chemistry or other chemistry, as is known
in the art. Such CNP variants can be generated using, e.g.,
wtCNP-22 (2.2 kDa), CNP-17 retaining only the cyclized region
(residues 6-22) of wtCNP22, CNP variants having an amino acid
extension at the N-terminus and/or C-terminus of CNP22, or variants
with amino acid substitutions, additions and/or deletions, for
example, GANRR-CNP22(K4R) (SEQ ID NO: 36), GANPR-CNP22(K4R) (SEQ ID
NO: 37), R-CNP22 (SEQ ID NO: 40), R-CNP22(K4R) (SEQ ID NO: 41),
ER-CNP22 (SEQ ID NO: 38) and ER-CNP22(K4R) (SEQ ID NO: 39). In an
embodiment, the PEG-CNP variants having a total mass characterized
by the ranges described generally herein, e.g., from about 2.6 kDa
or 2.8 kDa to about 6 or 7 kDa, contain a monodispersed, linear PEG
(or PEO) group conjugated at the N-terminus and/or C-terminus via
NHS- or aldehyde-based chemistry, or a two-arm or three-arm
branched PEG group conjugated at the N-terminus and/or C-terminus
via NHS-based chemistry. The disclosure further contemplates
negatively charged PEG-CNP variants designed for reduced renal
clearance, including but not limited to carboxylated, sulfated and
phosphorylated compounds.
In a related embodiment, the disclosure contemplates PEG-CNP
conjugates comprising NHS- or aldehyde-based PEG of the formula
(CH.sub.2CH.sub.2O).sub.n, wherein n is an integer from 12 to 50,
and the PEG polymer is up to about 2.5 kDa in molecular weight. In
a specific embodiment, n is 12 or 24. In an embodiment, the
terminal hydroxyl group of the PEG polymer is capped with a
non-reactive group. In a particular embodiment, the capping group
is an alkyl group, e.g., a lower alkyl group such as methyl.
In an additional embodiment, the PEG polymers or derivatives
thereof have a polymer number-average molecular weight in the range
from about 0.4 kDa to about 2.5 kDa or from about 0.6 kDa to about
1.5 kDa.
In a further embodiment, the wtCNP or CNP variant peptide is
conjugated to a moiety including, e.g., bisphosphonates,
carbohydrates, hydrophobic acids (including fatty acids) or amino
acid sequences. Such amino acid sequences include for example
polyAsp or polyGlu useful in bone/cartilage targeting, or can be
derived from bone proteins with elucidated bone-targeting domains
or derivatives thereof, such as for example fusion proteins or
peptide sequences of osteopontin, osteocalcin, sialoprotein, etc.
In embodiments described herein where CNP22 or a variant thereof is
attached to a bone- or cartilage-targeting moiety, such a moiety is
designed to promote getting the modified CNP peptide to
chondrocytes of bone growth plates, where the peptide can bind and
activate NPR-B on the chondrocytes.
In another embodiment, the disclosure provides CNP variants with a
peptide bond that is less susceptible to cleavage by peptidases
including NEP. The disclosure encompasses a CNP variant comprising
at least one modified residue at a site of endopeptidase cleavage.
In one embodiment, the Cys6-Phe7 peptide bond (--C(.dbd.O)--NH--)
at an NEP cleavage site in CNP can be replaced with anyone of the
following peptide-bond isosteres:
--CH.sub.2--NH--,
--C(.dbd.O)--N(R)--, where the amide group is alkylated with any of
the following R groups: methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl,
--C(.dbd.O)--NH--CH.sub.2--,
--CH.sub.2--S--,
--CH.sub.2--S(O).sub.n--, where n is 1 or 2,
--CH.dbd.CH--,
--C(.dbd.O)--CH.sub.2--,
--CH(CN)--NH--,
--CH(OH)--CH.sub.2--,
--O--C(.dbd.O)--NH--, and
--NHC(.dbd.O)NH--.
In another embodiment, Phe7 is substituted with its enantiomer
D-Phe. In yet another embodiment, D-enantiomers are introduced to
one or more, up to all 22, positions within wtCNP-22. In a further
embodiment, a beta amino acid such as 3-amino-2-phenylpropionic
acid is substituted for Phe7, which increases the length of the
backbone while reducing the length of the side chain. In yet
another embodiment, the disclosure contemplates Cys analogs at the
Cys6 position including but not limited to homocysteine,
penicillamine, 2-mercaptopropionic acid, and 3-mercaptopropionic
acid.
Even in the presence of a NEP-resistant bond between Cys6 and Phe7,
other peptide bonds can be hydrolyzed by NEP, including Gly8-Leu9,
Lys10-Leu11, Arg13-Ile14, Ser16-Met17 and Gly19-Leu20. Accordingly,
the disclosure encompasses CNP analogs containing peptide bond
isosteres at multiple locations in the backbone of the CNP analogs.
In one embodiment, CNP analogs or variants comprise modifications
at more than one peptidase cleavage site. In a further embodiment,
such variant comprises a CNP with substitutions at amino acid
residues important in binding to the NEP active site, thereby
increasing resistance to NEP degradation. One or more NEP-binding
residues, including but not limited to Gly8, Gly15, Ser18, Gly19
and/or Gly21, are replaced with larger-size natural or unnatural
amino acid residues to reduce affinity to the NEP active site. In
yet another embodiment, one or more hydrophobic residues essential
in NEP recognition, including but not limited to Phe7, Leu9, Leu11,
Ile14, Met17 and Leu20, are substituted with natural or unnatural
amino acids and/or peptidomimetics that decrease NEP binding. In
yet another embodiment, one to five of the first five amino acids
of CNP can be deleted or substituted with any other natural amino
acids or unnatural amino acids or peptidomimetics, or one or more
natural or unnatural amino acids or peptidomimetics can be added to
any one or to all of the first five positions of CNP.
In a further embodiment, the CNP variants have a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa for increased
resistance to NEP, and are represented by the formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(a).sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub.12-Arg.sub.13-(h).sub.1-
4-Gly.sub.15-Ser.sub.16-(i).sub.17-Ser.sub.18-Gly.sub.19-(j).sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 46), wherein:
(x) may be absent or may be selected from the group consisting of
synthetic bone-targeting compounds such as, e.g., bisphosphonates;
amino acid sequences useful in bone or cartilage targeting such as,
e.g., polyAsp and polyGlu; amino acid sequences derived from bone
proteins with elucidated bone-targeting domains, such as, e.g.,
fusion proteins or peptide sequences of osteopontin, osteocalcin,
sialoprotein, etc.; polymeric or non-polymeric molecules that
reduce renal clearance such as, e.g., charged PEG molecules; and
extensions comprising, e.g., polymers (e.g., PEGs), carbohydrates,
hydrophobic acids (including fatty acids), and/or amino acids, and
wherein such amino acid extensions can contain, e.g., from 1 to 31,
or 1 to 35, or 5 to 35, or 10 to 35, or 15 to 35 amino acid
residues, and can be derived from NPPC, ANP, BNP, other non-CNP
(poly)peptides such as, e.g., serum albumin or IgG, or variants of
the aforementioned polypeptides having substitutions, additions
and/or deletions, or combinations thereof;
(z) may be absent or may be selected from the group consisting of
amino acid sequences useful in bone or cartilage targeting such as
for example polyAsp and polyGlu, amino acid sequences from
bone-targeting domains of bone proteins such as, e.g., osteopontin,
osteocalcin and sialoprotein, and amino acid sequences derived from
non-CNP (poly)peptides such as, e.g., ANP or BNP;
(a) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (a) is Arg;
(b) is selected from the group consisting of Cys and peptide-bond
isosteres between Cys6 and Phe7 such as, e.g.,
Cys-CH.sub.2--NH;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; N-alkylated derivatives of Phe,
wherein the N-alkyl group is methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl; and Phe
analogs, wherein one or more ortho-, meta-, and/or para-positions
of the benzene ring of the Phe analog are substituted with one or
more substituents selected from the group consisting of halogen,
hydroxyl, cyano, straight or branched C.sub.1-6alkyl, straight or
branched C.sub.1-6alkoxy, straight or branched halo-C.sub.1-6
alkyl, C.sub.3-10 cycloalkyl, heterocyclyl, C.sub.6-14 aryl and
heteroaryl (examples include, but are not limited to, tyrosine,
3-chlorophenylalanine, 2,3-chloro-phenylalanine,
3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly
(tBu-Gly), Thr, Ser, Val and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr and
peptide-bond isosteres such as, e.g., N-Me-Leu;
(f) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (f) is not Arg;
(g) is selected from the group consisting of Leu and peptide-bond
isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tBu-Gly, and
peptide-bond isosteres such as, e.g., N-Me-Ile;
(i) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(j) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg,
and peptide-bond isosteres such as, e.g., N-Me-Leu.
In one embodiment, the CNP variants comprise a modification at one
or more of positions 6, 7, 8, 9, 10, 11, 13, 14, 16, 17, 19 and/or
20, and may optionally have modifications at any of the other
positions disclosed herein.
In embodiments described herein where CNP22 or variants thereof can
be attached to a hydrophobic acid, the CNP peptides can be attached
to one or hydrophobic acids. Non-limiting examples of hydrophobic
acids include straight-chain or branched, saturated or unsaturated
C.sub.5-C.sub.12 carboxylic acids (e.g., pentanoic acid, heptanoic
acid, etc.) and natural fatty acids. The hydrophobic acids can be
attached to the N-terminus, the C-terminus, and/or the side chain
of one or more amino acid residues. In one embodiment, the
hydrophobic acids are conjugated to the N-terminus. In an
embodiment, conjugation of CNP22 or a variant thereof to a
hydrophobic acid is designed, inter alia, to promote non-specific
interaction between the modified CNP peptide and serum albumin,
thereby increasing the size of the CNP peptide and protecting it
from cleavage by proteases such as, e.g., NEP. The interaction
between the hydrophobic acid-conjugated CNP peptide and albumin is
designed to be not too strong, so that the modified CNP peptide can
diffuse through cartilage, get to chondrocytes of bone growth
plates, and bind and activate NPR-B.
In a further embodiment, the disclosure provides CNP variants that
in vitro or in vivo stimulate the production of at least about 50%,
60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140% or 150% of the
cGMP level produced under the same concentration of wtCNP22 (e.g.,
1 uM), comprise at least one modified amino acid at position
(b).sub.6, (c).sub.7 and/or (d).sub.8, and are represented by the
general formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(a).sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub.12-Arg.sub.13-(h).sub.1-
4-Gly.sub.15-Ser.sub.16-(i).sub.17-Ser.sub.18-Gly.sub.19-(j).sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 47), wherein:
(x) may be absent or may be a peptide sequence containing one to
five amino acids which is derived from a natriuretic polypeptide
(e.g. NPPC, CNP, ANP or BNP) or a non-natriuretic polypeptide as
described herein (e.g., HSA, IgG, a bone-targeting protein,
etc.);
(z) may be absent or may be selected from the group consisting of
synthetic bone-targeting compounds such as, e.g., bisphosphonates;
amino acid sequences useful in bone/cartilage targeting such as,
e.g., polyAsp and polyGlu; bone proteins with bone-targeting
domains and derivatives thereof, such as fusion proteins or
peptides sequences of osteopontin, osteocalcin, and sialoprotein;
molecules that reduce renal clearance, such as, e.g., charged PEGs;
and molecules that increase resistance of CNP to NEP-mediated
degradation, as described herein;
(a) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (a) is Arg;
(b) may be Cys or descarboxy cysteine, or the (b).sub.6-(c).sub.7
peptide bond (--C(.dbd.O)--NH--) may be replaced with any one of
the following peptide bond isosteres: --CH.sub.2--NH--,
--C(.dbd.O)--N(R)--, where the amide group is alkylated with any of
the following R groups: methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl,
--C(.dbd.O)--NH--CH.sub.2--, --CH.sub.2--S--,
--CH.sub.2--S(O).sub.n--, where n is 1 or 2,
--CH.sub.2--CH.sub.2--, --CH.dbd.CH--, --C(.dbd.O)--CH.sub.2--,
--CH(CN)--NH--, --CH(OH)--CH.sub.2--, --O--C(.dbd.O)--NH--, or
--NHC(.dbd.O)NH--;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; peptide bond isosteres of Phe such
as N-alkylated derivatives of Phe wherein the N-alkyl group is
selected from the group consisting of methyl, ethyl, n-propyl,
isopropyl, cyclopropyl, n-butyl, isobutyl, sec-butyl and
tert-butyl; and Phe analogs, wherein one or more ortho-, meta-,
and/or para-positions of the benzene ring of the Phe analog are
substituted with one or more substituents selected from the group
consisting of halogen, hydroxyl, cyano, straight or branched
C.sub.1-6 alkyl, straight or branched C.sub.1-6 alkoxy, straight or
branched halo-C.sub.1-6 alkyl, C.sub.3-10 cycloalkyl, C.sub.6-14
aryl, heterocyclyl and heteroaryl (examples include, but are not
limited to, tyrosine, 3-chlorophenylalanine,
2,3-chloro-phenylalanine, 3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly
(tBu-Gly), Val, Ser, Thr and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr, and
peptide-bond isosteres such as, e.g., N-Me-Leu;
(f) is any natural or unnatural amino acid or peptidomimetic that
does not have a reactive primary amino group on a side chain,
including but not limited to Arg, Gly, 6-hydroxy-norleucine,
citrulline (Cit), Gln, Ser or Glu, wherein in one embodiment (f) is
not Arg;
(g) is selected from the group consisting of Leu and peptide-bond
isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tBu-Gly and
peptide-bond isosteres such as, e.g., N-Me-Ile;
(i) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(j) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg,
and peptide-bond isosteres such as, e.g., N-Me-Leu.
In a further embodiment, the disclosure encompasses CNP variants
that in vitro or in vivo stimulate the production of at least about
50%, 60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140% or 150% of
the cGMP level produced under the same concentration of wtCNP22
(e.g., 1 uM), have a total mass characterized by the ranges
described generally herein, e.g., from about 2.6 kDa or 2.8 kDa to
about 6 or 7 kDa for increased resistance to NEP degradation, and
are represented by the general formula:
(x)-(y)-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-(h).sub.10-Leu.s-
ub.11-Asp.sub.12-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Se-
r.sub.18-Gly.sub.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID
NO: 48), wherein:
(x) is a synthetic or natural polymeric group, or a combination
thereof, wherein a non-limiting example of a synthetic polymeric
group is polyethylene glycol (PEG), and a non-limiting example of a
natural polymeric group is an amino acid sequence containing from 1
to 35 amino acids and derived from NPPC or variants thereof with
substitutions and/or deletions, ANP, BNP, or other non-CNP
(poly)peptides such as, e.g., serum albumin, IgG, histidine-rich
glyco-proteins, fibronectin, fibrinogen, zinc finger-containing
polypeptides, osteocrin or FGF2;
(y) may be absent or may be one or more amino acids from
Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5 (corresponding to
positions 1 to 5 of CNP22) (SEQ ID NO: 1) and/or substitutions at
one or more of those positions using natural or unnatural amino
acids (e.g., K4R substitution);
(h) may be the wild-type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (h) is not Arg; and
(z) may be absent or may be a synthetic or natural polymeric group,
or a combination thereof, wherein a non-limiting example of a
synthetic polymeric group is PEG, and a non-limiting example of a
natural polymeric group is an amino acid sequence derived from a
natriuretic polypeptide (e.g., NPPC, CNP, ANP or BNP) or
non-natriuretic polypeptide (e.g., serum albumin or IgG).
In an embodiment, (x), (y) and (z) together contain from about 10
to about 40, or from about 15 to about 35 amino acids. In another
embodiment, (x) is an amino acid sequence comprising from 1 to 40
amino acids, or from 1 to 20 amino acids.
Further contemplated are CNP variants that in vitro or in vivo
stimulate the production of at least about 50%, 60%, 70%, 80%, 90%,
100%, 110%, 120%, 130%, 140% or 150% of the cGMP level produced
under the same concentration of wtCNP22 (e.g., 1 uM), and comprise
the sequence:
(y)-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub-
.12-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.-
sub.19-Leu.sub.20-Gly.sub.21-Cys.sub.22 (SEQ ID NO: 138),
wherein:
(y) comprises one or more amino acids selected from
Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5 (SEQ ID NO: 1)
and/or substitutions at one or more of those positions using
natural or unnatural amino acids (e.g., K4R substitution), and
further comprises a hydrophilic or water soluble polymer of
molecular weight from about 0.6 kDa to about 5 kDa. In an
embodiment, the hydrophilic or water-soluble polymer is conjugated
to the N-terminus of such amino acid-extended CNP variant. In a
particular embodiment, the hydrophilic or water-soluble polymer is
PEG (or PEO).
In yet another embodiment, the disclosure provides CNP variants
that in vitro or in vivo stimulate the production of at least about
50%, 60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140% or 150% of
the cGMP level produced under the same concentration of wtCNP22
(e.g. 1 uM), wherein the CNP variants comprise an N-terminal and/or
C-terminal peptide extension containing from 1 to 15 amino acids,
and are conjugated to a hydrophilic or water soluble polymer. In an
embodiment, the peptide extension contains from 5 to 10 amino
acids. In a specific embodiment, the peptide extension contains 5
amino acids. In another specific embodiment, the hydrophilic or
water-soluble polymer is PEG (or PEO).
In a still further embodiment, the CNP variants of the disclosure
in vitro or in vivo stimulate the production of at least about 50%,
60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140% or 150% of the
cGMP level produced under the same concentration of wtCNP22 (e.g. 1
uM), and comprise at least a 15 amino acid fragment derived from
natriuretic peptide precursor C(NPPC), wherein the fragment is at
least 70% homologous to a sequence from wild type NPPC containing
the same number of amino acid residues.
In still another embodiment, the CNP variants have a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa for increased NEP
resistance, and are represented by the formula:
(x)-(b).sub.6-(c).sub.7-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub-
.12-Arg.sub.13-(h).sub.14-Gly.sub.15-Ser.sub.16-(i).sub.17-Ser.sub.18-Gly.-
sub.19-(j).sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID NO: 49),
wherein:
(x) may be absent (i.e., the N-terminus ends with an --NH.sub.2
group) or may be selected from the group consisting of a sequence
of 1, 2, 3, 4 or 5 amino acids from the peptide
Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5 (SEQ ID NO: 1);
amino acid sequences useful in bone/cartilage targeting such as for
example polyAsp or polyGlu; bone-targeting domains from bone
proteins such as for example osteopontin, osteocalcin or
sialoprotein; molecules that reduce renal clearance such as
hydrophilic or water-soluble polymers, including but not limited to
charged PEG molecules; and moieties comprising PEG, carbohydrates,
hydrophobic acids, amino acids, or combinations thereof, wherein
such moieties can be amino acid extensions including but not
limited to amino acid sequences derived from NPPC or non-CNP
(poly)peptides such as, e.g., BNP, ANP, serum albumin or IgG;
(z) may be absent or may be selected from the group consisting of
amino acid sequences useful in bone/cartilage targeting such as for
example polyAsp or polyGlu; amino acid sequences derived from
bone-targeting proteins, such as for example osteopontin,
osteocalcin or sialoprotein; and amino acid sequences derived from
NPPC or non-CNP (poly) peptides, as described herein;
(b) is selected from the group consisting of Cys and peptide bond
isosteres between Cys6 and Phe7 such as, e.g.,
Cys-CH.sub.2--NH;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; N-alkylated derivatives of Phe,
wherein the N-alkyl group is methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl; and Phe
analogs, wherein one or more ortho-, meta-, and/or para-positions
of the benzene ring of the Phe analog are substituted with one or
more substituents selected from the group consisting of halogen,
hydroxyl, cyano, straight or branched C.sub.1-6 alkyl, straight or
branched C.sub.1-6 alkoxy, straight or branched halo-C.sub.1-6
alkyl, C.sub.3-10 cycloalkyl, C.sub.6-14 aryl, heterocyclyl and
heteroaryl (examples include, but are not limited to, tyrosine,
3-chlorophenylalanine, 2,3-chloro-phenylalanine,
3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly
(tBu-Gly), Val, Ser, Thr and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr, and
peptide-bond isosteres such as, e.g., N-Me-Leu;
(f) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (f) is not Arg;
(g) is selected from the group consisting of Leu and peptide-bond
isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tBu-Gly and
peptide-bond isosteres such as, e.g., N-Me-Ile;
(i) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(j) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg,
and peptide-bond isosteres such as, e.g., N-Me-Leu.
In a further embodiment, the CNP variants have a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa for increased NEP
resistance, and are represented by the formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(a).sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub.12-Arg.sub.13-(h).sub.1-
4-(i).sub.15-Ser.sub.16-(j).sub.17-Ser.sub.18-Gly.sub.19-(k).sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 50), wherein:
(x) and (z) independently may be absent or may be selected from the
group consisting of synthetic bone-targeting compounds such as,
e.g., bisphosphonates; amino acid sequences useful in
bone/cartilage targeting such as for example polyAsp or polyGlu;
amino acid sequences derived from bone-targeting domains of bone
proteins and derivatives thereof, such as for example fusion
proteins or peptide sequences of osteopontin, osteocalcin,
sialoprotein, etc.; moieties that reduce renal clearance, including
but not limited to hydrophilic or water-soluble polymers such as,
e.g., charged PEG molecules; and moieties comprising, e.g.,
hydrophilic polymers (e.g., PEG), carbohydrates, hydrophobic acids,
and/or amino acids;
(a) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (a) is Arg;
(b) is selected from the group consisting of Cys and peptide bond
isosteres between Cys6 and Phe7 such as, e.g.,
Cys-CH.sub.2--NH;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; N-alkylated derivatives of Phe,
wherein the N-alkyl group is methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl; and Phe
analogs, wherein one or more ortho-, meta-, and/or para-positions
of the benzene ring of the Phe analog are substituted with one or
more substituents selected from the group consisting of halogen,
hydroxyl, cyano, straight or branched C.sub.1-6 alkyl, straight or
branched C.sub.1-6 alkoxy, straight or branched halo-C.sub.1-6
alkyl, C.sub.3-10 cycloalkyl, C.sub.6-14 aryl, heterocyclyl and
heteroaryl (examples include, but are not limited to, tyrosine,
3-chlorophenylalanine, 2,3-chloro-phenylalanine,
3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly,
Thr, Ser, Val and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr, and
peptide bond isosteres such as, e.g., N-Me-Leu;
(f) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (f) is not Arg;
(g) is selected from the group consisting of Leu, Asn, and peptide
bond isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tert-butyl-Gly
(tBu-Gly), Asn, and peptide bond isosteres such as, e.g.,
N-Me-Ile;
(i) is selected from the group consisting of Gly, Arg, Ser and
Asn;
(j) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(k) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Arg, Thr, Ser,
and peptide bond isosteres such as, e.g., N-Me-Leu.
In a further embodiment, the CNP variants can have amino acid
substitution(s) at one or more of any of positions 1 to 22 of
CNP22. In one embodiment, Gly1 is substituted with Arg or Glu. In
another embodiment, Lys4 is replaced with Arg. In still another
embodiment, Gly5 is substituted with Arg, Gln or Ser. In yet
another embodiment, Gly15 is substituted with Ser, Asn, Arg or Cit.
In a further embodiment, Gly19 is substituted with Ser, Arg or Asn.
In yet another embodiment, Gly21 is substituted with Ser, Thr, or
Arg.
In one embodiment, the CNP variant is selected from the group
consisting of GLSKGC(CH.sub.2NH)FGLKLDRIGSMSGLGC (formed using
descarboxy-Cys) (SEQ ID NO: 56), GLSKGC-(N-Me-Phe)-GLKLDRIGSMSGLGC
(SEQ ID NO: 57), GLSKGC-(D-Phe)-GLKLDRIGSMSGLGC(SEQ ID NO:136),
GLSKGCF-(tBuG)-LKLDRIGSMSGLGC (SEQ ID NO: 58),
GLSKGC-(3-Cl-Phe)-GLKLDRIGSMS-GLGC (SEQ ID NO:137), and
GLSKGC-[NHCH.sub.2CH(Ph) CO]-GLKLDRIGSMSGLGC (formed using
3-amino-2-phenylpropionic acid) (SEQ ID NO: 59). In a further
embodiment, a disulfide bond exists between Cys6, descarboxy-Cys or
another sulfhydryl-containing cysteine analog at the Cys6 position,
and Cys22 of any CNP variant described herein.
In another embodiment, the CNP variants contain an amino acid
extension at the N-terminus and/or C-terminus of CNP22 or CNP17,
including but not limited to:
TABLE-US-00004 (SEQ ID NO: 4)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC (CNP-53);
(SEQ ID NO: 60) QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-37,
Analog BL); (SEQ ID NO: 61) AAWARLLQEHPNAGLSKGCFGLKLDRIGSMSGLGC
(Analog CA); (SEQ ID NO: 62) AAWARLLQEHPNARGLSKGCFGLKLDRIGSMSGLGC
(Analog CB); (SEQ ID NO: 63) DLRVDTKSRAAWARGLSKGCFGLKLDRIGSMSGLGC
(Analog CC); (SEQ ID NO: 40) RGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO:
38) ERGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 64)
GANQQGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 65)
GANRRGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 66)
GANPRGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 67)
GANSSGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 144)
GHKSEVAHRFKGANKKGLSKGCFGLKLDRIGSMSGLGC (sometimes designated
"CNP27-HSA" or "HSA-CNP27" in the Examples and figures); (SEQ ID
NO: 68) SPKMVQGSG-CNP17-KVLARH (Analog CD) (CNP17 having N-terminal
and C-terminal tails derived from BNP).
In a further embodiment, the CNP variants have a K4R substitution
at position 4 of CNP22. Non-limiting examples of CNP(K4R) variants
include:
TABLE-US-00005 ((SEQ ID NO: 36) GANRRGLSRGCFGLKLDRIGSMSGLGC (Analog
AY); (SEQ ID NO: 37) GANPRGLSRGCFGLKLDRIGSMSGLGC (Analog CI); (SEQ
ID NO: 41) RGLSRGCFGLKLDRIGSMSGLGC (Analog AZ); (SEQ ID NO: 39)
ERGLSRGCFGLKLDRIGSMSGLGC (Analog BA); (SEQ ID NO: 69)
GANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CH); and (SEQ ID NO: 70)
GANSSGLSRGCFGLKLDRIGSMSGLGC (Analog CG).
In one embodiment, CNP variants having a PEG (or PEO) moiety and an
amino acid extension at the N-terminus contain arginine at the
position immediately preceding the position corresponding to Gly1
of CNP22. Such PEGylated CNP variants are designed for increased
resistance to NEP degradation, reduced binding to serum albumin,
and enhanced CNP functional activity (e.g., activation of cGMP
signaling). Non-limiting examples of PEGylated CNP variants include
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36), PEO12-GANRR-CNP22(K4R) (SEQ
ID NO: 36), PEO24-GANRR-CNP22(SEQ ID NO: 65), PEO12-GANRR-CNP22(SEQ
ID NO: 65), PEO24-GANPR-CNP22(K4R) (SEQ ID NO: 37),
PEO12-GANPR-CNP22(K4R) (SEQ ID NO: 37), PEO24-GANPR-CNP22(SEQ ID
NO: 37), PEO12-GANPR-CNP22 (SEQ ID NO: 66), PEO24-GANQQ-CNP22(SEQ
ID NO: 64), PEO12-GANQQ-CNP22(SEQ ID NO: 64), PEO24-ER-CNP22(K4R)
(SEQ ID NO: 39), PEO12-ER-CNP22(K4R) (SEQ ID NO: 39),
PEO24-ER-CNP22(SEQ ID NO: 38), PEO12-ER-CNP22(SEQ ID NO: 38),
PEO24-R-CNP22(K4R) (SEQ ID NO: 41), PEO12-R-CNP22(K4R) (SEQ ID NO:
41), PEO24-R-CNP22(SEQ ID NO: 40), and PEO12-R-CNP22(SEQ ID NO:
40), wherein PEO24 is a monodispersed 1.2 kDa PEG polymer and PEO12
is a monodispersed 0.6 kDa PEG polymer. In an embodiment, the PEG
(or PEO) polymer is attached to the N-terminus of the CNP
variants.
Additional CNP variants include, but are not limited to,
derivatives of CNP37 having mutation(s) at the furin cleavage site
(underlined), designed to improve in vivo resistance to the furin
protease, and/or having glycine (underlined) preceding glutamine,
designed to prevent pyroglutamine formation, including but not
limited to:
TABLE-US-00006 (SEQ ID NO: 71)
GQEHPNARKYKGANPKGLSKGCFGLKLDRIGSMSGLGC (An. CS); (SEQ ID NO: 72)
GQEHPNARKYKGANQKGLSKGCFGLKLDRIGSMSGLGC (An. CT); (SEQ ID NO: 73)
GQEHPNARKYKGANQQGLSKGCFGLKLDRIGSMSGLGC (An. CU); (SEQ ID NO: 74)
GQEHPNARKYKGANKPGLSKGCFGLKLDRIGSMSGLGC (An. CW); (SEQ ID NO: 75)
GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Gly-CNP37, An. DB); (SEQ ID
NO: 145) PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC
(Pro-Gly-CNP37).
In another embodiment, the CNP variants are chimera comprising
CNP22 and an N-terminal peptide fragment, including but not limited
to:
TABLE-US-00007 (SEQ ID NO: 76)
GHHSHEQHPHGANQQGLSKGCFGLKLDRIGSMSGLGC (Analog CQ) (histidine-rich
glycoprotein (HRGP) fragment- CNP22 chimera); (SEQ ID NO: 77)
GAHHPHEHDTHGANQQGLSKGCFGLKLDRIGSMSGLGC (Analog CR) (HRGP
fragment-CNP22 chimera); (SEQ ID NO: 78)
GHHSHEQHPHGANPRGLSKGCFGLKLDRIGSMSGLGC (Analog CX) (HRGP
fragment-CNP22 chimera); (SEQ ID NO: 79)
GQPREPQVYTLPPSGLSKGCFGLKLDRIGSMSGLGC (Analog CF) (IgG.sub.1(F)
fragment-CNP22 chimera); (SEQ ID NO: 80)
GQHKDDNPNLPRGANPRGLSKGCFGLKLDRIGSMSGLGC (Analog CY) (human serum
albumin (HSA) fragment-CNP22 chimera); (SEQ ID NO: 81)
GERAFKAWAVARLSQGLSKGCFGLKLDRIGSMSGLGC (Analog CE) (HSA
fragment-CNP22 chimera); (SEQ ID NO: 82)
FGIPMDRIGRNPRGLSKGCFGLKLDRIGSMSGLGC (Analog CZ) (osteocrin "NPR C
inhibitor" fragment-CNP22 chimera); and (SEQ ID NO: 83)
GKRTGQYKLGSKTGPGPKGLSKGCFGLKLDRIGSMSGLGC (Analog DA) (FGF2
"heparin-binding domain" fragment-CNP22 chimera).
In a further embodiment, the CNP variants are chimera comprising an
N-terminal peptide fragment and CNP22 in which arginine is
substituted for Lys4 of CNP22 ("CNP22(K4R)"), including but not
limited to:
TABLE-US-00008 (SEQ ID NO: 84)
GQPREPQVYTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CK) (IgG.sub.1(F)
fragment-CNP22(K4R) chimera); (SEQ ID NO: 85
GVPQVSTSTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CL) (HSA
fragment-CNP22(K4R) chimera) (SEQ ID NO: 86)
GQPSSSSQSTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CM) (fibronectin
fragment-CNP22(K4R) chimera); (SEQ ID NO: 87)
GQTHSSGTQSGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CN) (fibrinogen
fragment-CNP22(K4R) chimera); (SEQ ID NO: 88)
GSTGQWHSESGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CO) (fibrinogen
fragment-CNP22(K4R) chimera); and (SEQ ID NO: 89)
GSSSSSSSSSGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CP) (zinc finger
fragment-CNP22(K4R) chimera).
In yet another embodiment, the CNP variants are chimera, or fusion
proteins, comprising a CNP peptide or variant, and a cleavable
peptide or protein, or peptide tag. Exemplary cleavable proteins or
peptides include, but are not limited to, histidine (e.g.,
hexa-His) tags; TAF12: human transcription factor TAF12; KSI:
ketosteroid isomerase; MBP: maltose-binding protein; .beta.-Gal:
.beta.-galactosidase; GST: glutathione-S-transferase; Trx:
thioredoxin; CBD: chitin binding domain; BMPM: BMP-2 mutation,
SUMO, CAT, TrpE, staphylococcal protein A, streptococcal proteins,
starch-binding protein, cellulose-binding domain of endoglucanase
A, cellulose-binding domain of exoglucanase Cex, biotin-binding
domain, recA, Flag, c-Myc, poly(His), poly(Arg), poly(Asp), poly
(Gln), poly(Phe), poly(Cys), green fluorescent protein, red
fluorescent protein, yellow fluorescent protein, cyan fluorescent
protein, biotin, avidin, streptavidin, antibody epitopes, and
fragments thereof.
In yet another embodiment, the CNP variant may be a monomer or a
dimer. In a related embodiment the monomers of dimeric CNP variants
can be attached N-terminus to N-terminus via a linker or no linker,
N-terminus to C-terminus via a linker or no linker, or C-terminus
to C-terminus via a linker or no linker.
Chimera comprising an IgG fragment and CNP22 or a variant thereof
are designed for, inter alia, increased resistance to NEP
degradation and reduced binding to serum albumin. CNP chimera
comprising a surface fragment of HSA are designed for, inter alia,
reduced immunogenicity and reduced binding to serum albumin.
HRGP-CNP22 and HRGP-CNP22(K4R) chimera containing a cationic,
histidine-rich, non-lysine, non-arginine sequence at the N-terminus
are designed for, inter alia, increased stability to proteases.
Chimera containing an osteocrin fragment are designed to release,
upon protease (e.g., furin) cleavage, the osteocrin fragment at
bone growth plates, where the fragment would inhibit the clearance
receptor NPR-C. With respect to chimera comprising an FGF2
heparin-binding fragment, heparin binding to the fragment is
designed to protect the chimera from degradation, thereby providing
a longer serum half-life. Chimera containing a fibronectin,
fibrinogen, or zinc-finger fragment are designed for reduced
binding to serum albumin, among other advantageous features.
Not intending to be bound by theory, a CNP variant of molecular
weight from about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa which has
increased resistance to NEP degradation and has similar or improved
functionality (e.g., binding to NPR-B and stimulation of cGMP
signaling) as compared to wtCNP22, may be more effective if it does
not bind tightly to plasma proteins such as serum albumin. A CNP
variant that does not bind tightly to plasma proteins (e.g., serum
albumin) may be more effective in diffusing through cartilage,
getting to chondrocytes of bone growth plates, and binding to and
activating NPR-B for cGMP signaling. In one embodiment, CNP
variants designed for reduced binding to plasma proteins (e.g.,
serum albumin) are chimeras comprising CNP22 or a variant thereof
and a peptide fragment from IgG. In another embodiment, CNP
variants designed for reduced binding to plasma proteins are
chimeras comprising CNP22 or CNP22(K4R) and a fragment from a
polypeptide (e.g., IgG, HSA, fibronectin, fibrinogen, a zinc
finger-containing polypeptide, etc.). In yet another embodiment,
CNP variants designed for reduced binding to plasma proteins
comprise CNP22 or a variant thereof conjugated to a hydrophilic or
water-soluble polymer. In one embodiment, the hydrophilic or
water-soluble polymer is PEG (or PEO). In another embodiment, the
hydrophilic or water-soluble polymer (e.g., PEG) is functionalized
with one or more functional groups that impart a negative charge to
the polymer under physiological conditions, such as, e.g, carboxyl,
sulfate or phosphate groups, or a combination thereof.
In any of the embodiments disclosed herein, the CNP variants may
have substantially the same or better biological activity than
wild-type CNP22. For example, the CNP variants may retain at least
50%, 60%, 70%, 80%, 90%, 95% or more of the activity of wild-type
CNP22, or may have greater activity than CNP22, e.g., with respect
to interaction with NPR-B (GC-B) to stimulate the generation of
cGMP. Alternatively, or in addition, the CNP variants may retain at
least 50%, 60%, 70%, 80%, 90%, 95% or more of the activity of
wild-type CNP22, or may have greater activity than CNP22, with
respect to regulating endochondral bone growth and chondrocyte
activity, including but not limited to chondrocyte proliferation,
chondrocyte differentiation, inhibition of the mitogen activated
protein (MAP) kinase/MEK (Raf-1) kinase signaling pathway, and
promoting endochondral ossification. In any of the embodiments
described herein, the CNP variants may comprise an amino acid
sequence that is at least 40%, 50%, 60%, 70%, 80%, 90%, 95% or more
identical or homologous to amino acids 6-22 or 1-22 of wild-type
CNP22.
In a further embodiment, the disclosure provides variants of CNP22
having less affinity to the NPR-C clearance receptor while
retaining the ability to bind and activate NPR-B. The present
disclosure encompasses variants that were, or can be, generated
from a homology-based structural model of the NPR-B/CNP complex as
described in the Detailed Description. In another embodiment, the
CNP variants have substitution(s) at one or more Gly sites at
positions 1, 5, 8, 15, 19 and 21, to reduce conformational
flexibility, which may increase their specificity for binding to
NPR-B over NPR-C. Variants of CNP having potentially reduced
affinity to the NPR-C include but are not limited to those having
one or more of the following substitutions: G1R, G1E, G5R, G5Q,
G5S, F7Y, G8T, G8S, G8V, G8N, L9S, L9T, K10Cit, K10Q, K10S, I14N,
G15R, G15S, G15N, G15Cit, S16Q, M17V, M17N, G19S, G19R, G19N, L20V,
L20R, L20T, L20S, G21S, G21T and G21R.
In yet another embodiment, the CNP variants have a modification
and/or substitution at one or more of positions 5, 7, 8, 9, 10, 14,
15, 16, 17, 19, 20 and 21, and may optionally have modifications
and/or substitutions at any of the other positions disclosed
herein. In a further embodiment, the CNP variants can optionally
have conjugation(s) or extension(s), e.g., at the N- and/or
C-terminus to facilitate bone/cartilage targeting, reduce renal
clearance, and/or increase resistance to NEP degradation. Such
conjugation(s) or extension(s) can comprise molecules or sequences
formed or derived from, e.g., polyAsp, polyGlu, bone- or
cartilage-targeting peptides, osteopontin, osteocalcin,
sialoprotein, PEGs, carbohydrates, hydrophobic acids, NPPC or
non-CNP (poly)peptides, or combinations thereof.
In still another embodiment, the CNP variants are prepared by
standard solid-phase peptide synthesis methods with natural or
unnatural amino acid(s) or peptidomimetic(s) being substituted
and/or added where appropriate. In another embodiment, the CNP
variants are produced by recombinant synthesis processes, e.g., via
fusion proteins containing a tag or carrier protein, wherein use of
the tag or carrier protein facilitates, e.g., detection, isolation
and/or purification of the fusion protein, and selective chemical
or proteolytic cleavage of the tag or carrier protein from the
fusion protein provides the target CNP variant. In a further
embodiment, PEGylation of the CNP variants occurs following, or
part of, chemical or biological synthesis with the conjugation
reaction being performed by NHS- or aldehyde-based chemistry or
other chemistry known in the art. In another embodiment, the CNP
variants comprise a disulfide bond. In a related embodiment, the
disulfide bond forms a cyclic peptide. In a particular embodiment,
the disulfide bond is formed between cysteine residues at positions
corresponding to positions 6 and 22 of CNP22.
It is further contemplated that the CNP variants can be conjugated
to a hydrophobic polymeric or non-polymeric moiety, such as, e.g.,
heptanoic acid, pentanoic acid, or fatty acids. The hydrophobic
moiety can be conjugated to the side chain of an amino acid
residue, including but not limited to a lysine, a serine, a
cysteine or a threonine, or can be attached to the N-terminus
and/or C-terminus of the CNP variant.
In an embodiment, the CNP variants as described herein have a pI in
the range from about 8 to about 10.5 or from about 8.5 to about
10.
In a further embodiment, the disclosure provides a pharmaceutical
composition comprising a CNP variant, optionally another
biologically active agent, and optionally a pharmaceutically
acceptable excipient, carrier or diluent. In some embodiments, the
compositions are sterile pharmaceutical compositions suitable for
parenteral injection. In some embodiments, the compositions
comprise substantially pure CNP variant, e.g. at least about 90% or
95% pure. In some embodiments, the compositions contain less than
about 5%, 4%, 3%, 2%, 1% or 0.5% contaminants, such as other human
proteins, porcine proteins, or CNP53 or fragments thereof (other
than the desired CNP variant). In certain embodiments, the sterile
composition is administered to a subject for treating or preventing
any of the CNP-responsive conditions or disorders disclosed
herein.
CNP variants of the disclosure advantageously retain CNP activity
and exhibit increased serum half-life. Retention of CNP activity
can be shown, for example, as retention of desired in vivo
biological effect, or retention of at least about 50%, 60%, 70%,
80%, 90%, 95% or at least about 100% of the cGMP stimulating
activity of CNP22, under the same concentration (e.g., 1 uM of CNP
peptide or greater than the ED80). In some embodiments, CNP
variants exhibit at least about 1.5-fold, 2-fold, 3-fold, 4-fold,
5-fold, 10-fold, 15-fold, 20-fold, 25-fold, 30-fold, 35-fold or
40-fold increase in serum half-life compared to CNP22.
In a related embodiment, the CNP variants described herein have
increased NEP resistance and exhibit increased half-life compared
to wild-type CNP22. In one embodiment, the half-life of the CNP
variants is increased by about 20%, 30%, 40%, 50%, 60%, 70%, 80%,
90%, 95%, 96%, 97%, 98%, 99% or about 100% compared to wild-type
CNP22.
In certain embodiments, the CNP variants described herein increase
cGMP production in vitro, increase cGMP production in vivo,
increase in vivo the level of one or more biomarkers associated
with cartilage or bone formation or growth, increase resistance to
NEP cleavage in vitro, increase plasma or serum half-life in vivo,
increase bioavailability in vivo, or increase the length of
particular bones in vivo, or effect combinations of such increases,
by about 1.5-fold, about 2-fold, about 2.5-fold, about 3-fold,
about 3.5-fold, about 4-fold, about 4.5-fold, or about 5-fold or
more compared to wild-type CNP22.
In yet another embodiment, the disclosure provides methods of
treating conditions or disorders responsive to CNP, comprising
administering a therapeutically effective amount of a CNP variant
or a composition comprising the same to a subject in need thereof.
In one embodiment, disorders responsive to CNP are disorders of
bone growth, including but not limited to skeletal dysplasias and
inherited skeletal malformations such as disorders associated with
fibroblast growth factor receptor 3 (FGFR-3) mutations. In a
specific embodiment, the disorder associated with FGFR-3
mutation(s) is achondroplasia. In another embodiment, the disorders
responsive to CNP are disorders associated with vascular smooth
muscle cells and tissues. In a further embodiment, the CNP variants
are useful for increasing the size of the growth plate of a bone
(e.g., a limb bone). In another embodiment, the CNP variants are
useful for elongating a bone or increasing long bone growth. In
still another embodiment, the CNP variants are useful for enhancing
matrix production, proliferation and differentiation of
chondrocytes.
In certain embodiments, the CNP variants described herein are
administered at a dose in the range from about 5 or 10 nmol/kg to
about 300 nmol/kg, or from about 20 nmol/kg to about 200 nmol/kg.
In some embodiments, the CNP variants are administered at a dose of
about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75,
80, 85, 90, 95, 100, 125, 150, 175, 200, 210, 220, 230, 240, 250,
260, 270, 280, 290, 300, 350, 400, 450, 500, 750, 1000, 1250, 1500,
1750 or 2000 nmol/kg or other dose deemed appropriate by the
treating physician. In other embodiments, the CNP variants are
administered at a dose of about 5, 10, 20, 30, 40, 50, 60, 70, 80,
90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250,
300, 350, 400, 450, 500, 550, 600, 650, 700, 750 or 800 ug/kg, or
about 1 mg/kg, 1.25 mg/kg, 1.5 mg/kg, 2 mg/kg or other dose deemed
appropriate by the treating physician. The doses of CNP variants
described herein can be administered according to the dosing
frequency/frequency of administration described herein, including
without limitation daily, 2 or 3 times per week, weekly, every 2
weeks, every 3 weeks, monthly, etc.
In another embodiment, the CNP variants are administered in a
single treatment or in multiple doses. The multiple doses may be
administered daily, or in multiple doses over the course of
treatment. In certain embodiments, it is contemplated that the CNP
variant is administered, in a single dose or in multiple doses,
daily, every other day, every 3 days, 2 times per week, 3 times per
week, weekly, bi-weekly, every 3 weeks, monthly, every 6 weeks,
every 2 months, every 3 months or as deemed appropriate by a
treating physician.
In certain embodiments, administration of the CNP variant is
adjusted to allow for periods of growth (e.g., chondrogenesis),
followed by a recovery period (e.g., osteogenesis). For example,
the CNP variant may be administered subcutaneously, intravenously,
or by another mode daily or multiple times per week for a period of
time, followed by a period of no treatment, then the cycle is
repeated. In some embodiments, the initial period of treatment
(e.g., administration of the CNP variant daily or multiple times
per week) is for 3 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 5
weeks, 6 weeks, 7 weeks, 8 weeks, 9 weeks, 10 weeks, 11 weeks or 12
weeks. In a related embodiment, the period of no treatment lasts
for 3 days, 1 week, 2 weeks, 3 weeks or 4 weeks. In certain
embodiments, the dosing regimen of the CNP variant is daily for 3
days followed by 3 days off; or daily or multiple times per week
for 1 week followed by 3 days or 1 week off; or daily or multiple
times per week for 2 weeks followed by 1 or 2 weeks off; or daily
or multiple times per week for 3 weeks followed by 1, 2 or 3 weeks
off; or daily or multiple times per week for 4, 5, 6, 7, 8, 9, 10,
11 or 12 weeks followed by 1, 2, 3 or 4 weeks off.
In additional embodiments, the disclosure provides a method of
treating a CNP-responsive condition or disorder, comprising
administering a CNP peptide or variant to a subject, and monitoring
the level of at least one bone- or cartilage-associated biomarker
in the subject (e.g., in a biological sample from the subject),
wherein an increase or decrease in the level of the bone- or
cartilage-associated biomarker indicates a therapeutic effect of
the CNP peptide or variant on the subject. In some embodiments,
when the level of a biomarker increases in association with bone or
cartilage formation or growth, an increase in the level of that
biomarker indicates a therapeutic effect of the CNP peptide or
variant on the subject. In other embodiments, when the level of a
biomarker decreases in association with bone or cartilage formation
or growth, a decrease in the level of that biomarker indicates a
therapeutic effect of the CNP peptide or variant on the
subject.
In further embodiments, the therapeutic method further comprises
adjusting the amount (or dose) or frequency of administration of
the CNP peptide or variant, wherein: (i) the amount (or dose) or
frequency of administration of the CNP peptide or variant is
increased if the level of the at least one bone- or
cartilage-associated biomarker is below a target level, where the
level of the biomarker increases in association with bone or
cartilage formation or growth; or (ii) the amount (or dose) or
frequency of administration of the CNP peptide or variant is
decreased if the level of the at least one bone- or
cartilage-associated biomarker is above a target level, where the
level of the biomarker increases in association with bone or
cartilage formation or growth; or (iii) the amount (or dose) or
frequency of administration of the CNP peptide or variant is
increased if the level of the at least one bone- or
cartilage-associated biomarker is above a target level, where the
level of the biomarker decreases in association with bone or
cartilage formation or growth; or (iv) the amount (or dose) or
frequency of administration of the CNP peptide or variant is
decreased if the level of the at least one bone- or
cartilage-associated biomarker is below a target level, where the
level of the biomarker decreases in association with bone or
cartilage formation or growth. It is contemplated that the target
level of a biomarker refers to the level or range of levels of the
biomarker that is associated with therapeutic effect in the subject
and/or beneficial effect in alleviating or ameliorating symptoms of
the disorder or condition. In certain embodiments, a level of a
biomarker above or below a target level may be deleterious to the
subject.
In other embodiments, the disclosure contemplates a method for
assessing the effect of administration of a CNP peptide or variant
on bone or cartilage formation or growth. In one embodiment, the
method provides for assaying or measuring the level of at least one
bone- or cartilage-associated biomarker in a subject that has been
administered a CNP peptide or variant in order to assess the effect
of the CNP peptide or variant on bone and cartilage formation and
growth in vivo. In a related embodiment, an increase in the level
of the at least one bone- or cartilage-associated biomarker may
indicate that administration of a CNP peptide or variant has a
positive effect on bone or cartilage formation or growth and is a
useful treatment for skeletal dysplasias and other bone- or
cartilage-related diseases or disorders associated with decreased
CNP activity. Exemplary bone- or cartilage-associated biomarkers
include, but are not limited to, CNP (e.g, endogenous level of
CNP-22 or CNP-53), cGMP, osteocalcin, proliferating cell nuclear
antigen (PCNA), propeptides of type I procollagen (PINP) and
fragments thereof, collagen type I and fragments thereof,
propeptides of collagen type II and fragments thereof, collagen
type II and fragments thereof, aggrecan chondroitin sulfate, and
alkaline phosphatase.
In further embodiments, the disclosure contemplates a method for
assessing the effect of a CNP peptide or variant on the level of at
least one bone- or cartilage-associated biomarker in a subject,
comprising assaying or measuring the level of the bone- or
cartilage-associated biomarker in a biological sample from a
subject that has been administered a CNP peptide or variant. In
some embodiments, the method further comprises administering the
CNP peptide or variant to the subject before assaying or measuring
the level of the bone- or cartilage-associated biomarker.
In certain embodiments, the at least one bone- or
cartilage-associated biomarker is selected from the group
consisting of CNP (e.g, endogenous level of CNP-22 or CNP-53),
cGMP, propeptides of collagen type II and fragments thereof,
collagen type II and fragments thereof, osteocalcin, proliferating
cell nuclear antigen (PCNA), propeptides of type I procollagen
(PINP) and fragments thereof, collagen type I and fragments
thereof, aggrecan chondroitin sulfate, and alkaline
phosphatase.
In some embodiments of methods (e.g., therapeutic, diagnostic and
assay methods) relating to bone- or cartilage-associated
biomarkers, the CNP peptide or variant is CNP-22, CNP-53, or any of
the CNP peptides and variants described herein. In certain
embodiments of such methods, the CNP peptide or variant is not
CNP-22 or CNP-53.
In other embodiments, the disclosure provides a method for
recombinant production of a CNP variant, comprising culturing in a
medium a host cell comprising a polynucleotide encoding a CNP
variant peptide linked to a polynucleotide encoding a cleavable
peptide or protein, under conditions that result in expression of a
fusion polypeptide encoded by the polynucleotides. In a related
embodiment, the host cell is transformed with an expression vector
comprising a polynucleotide encoding a CNP variant peptide linked
to a polynucleotide encoding a cleavable peptide or protein.
In one embodiment, the vector is a plasmid. In still another
embodiment, the plasmid is selected from the group consisting of
pET-21a, pJexpress, pET-31b, pET-15b, pET-32a, pET-41a, pMAL,
pQE-30, pET-SUMO, pET-22b, and pTYB11.
In certain embodiments, the cleavable peptide or protein comprises
a polypeptide that is selected from the group consisting of a
histidine tag, human transcription factor TAF12, ketosteroid
isomerase, maltose-binding protein, .beta.-galactosidase,
glutathione-S-transferase, thioredoxin, chitin binding domain, and
BMP-2 mutation, or fragments thereof.
In a related embodiment, the cleavable peptide or protein is
cleaved by a cleaving agent. In some embodiments, the cleaving
agent is selected from the group consisting of formic acid,
cyanogen bromide (CNBr), hydroxylamine, protein self cleavage,
Factor Xa, enterokinase, ProTEV, and SUMO protease. Additional
exemplary cleaving agents include, but are not limited to,
palladium, clostripain, thrombin, chymotrypsin, trypsin,
trypsin-like proteases, carboxypeptidase, enteropeptidase, Kex 2
protease, Omp T protease, subtilisin, V8 protease, HIV protease,
rhinovirus protease, furilisin protease, IgA proteases, human Pace
protease, collagenase, Nia protease, poliovirus 2Apro protease,
poliovirus 3C protease, genenase, furin, elastase, Proteinase K,
pepsin, rennin (chymosin), microbial aspartic proteases, papain,
calpain, chymopapain, ficin (ficain), bromelain (bromelase),
cathespisin B, caspases, thermolysin, Endoprotease Arg-C,
Endoprotease Glu-C, Endoprotease Lys-C, kallikrein, and
plasmin.
In certain embodiments, the fusion polypeptide is expressed as a
soluble protein or as an inclusion body. In a related embodiment,
the disclosure contemplates isolating the expressed fusion
polypeptide from the host cell or culture medium. In a further
embodiment, the isolated fusion polypeptide is contacted with a
cleaving agent as described herein.
In one embodiment, the disclosure provides a bacterial host cell
comprising an expression vector, said vector comprising a
polynucleotide encoding a CNP variant peptide linked to a
polynucleotide encoding a cleavable peptide or protein. In some
embodiments, the cleavable peptide or protein is selected from the
group consisting of a histidine tag, human transcription factor
TAF12, ketosteroid isomerase, maltose-binding protein,
.beta.-galactosidase, glutathione-S-transferase, thioredoxin,
chitin binding domain, and BMP-2 mutation, or fragments
thereof.
In another embodiment, the host cell is a bacteria, such as E.
coli. In a related embodiment, the E. coli cell is selected from
the group consisting of BL21(DE3), BL21(DE3)pLysS, BL21(DE3)pGro7,
ArcticExpress(DE3), C41 [also called C41(DE3)], C43 [also called
C43(DE3)], Origami B(DE3), Origami B(DE3)pLysS, KRX, and
Tuner(DE3). In still a further embodiment, the host cell comprises
a vector as described above. In some embodiments, the host cell is
transformed with the vector prior to cell culture.
In certain embodiments, it is contemplated that the host cell is
cultured in a medium under conditions suitable for expression of a
fusion polypeptide encoded by the polynucleotides. In one
embodiment, the fusion polypeptide is expressed as a soluble
protein or as inclusion body. In a related embodiment, the
expressed fusion polypeptide is isolated from the host cell or
culture medium. In still another embodiment, the isolated fusion
polypeptide is contacted with a cleaving agent as described
herein.
BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 shows expression of CNP fusion proteins in E. coli (FIG. 1A:
Coomassie blue stain, FIG. 1B: Western blot). M: protein marker; T:
total cell lysates; S: soluble supernatants; P: 2 ug CNP22; KSI:
KSI-CNP(M/N) fusion protein expression (insoluble); N: Un-induced
KSI-CNP fusion protein total lysates; KSI': KSI-P-CNP fusion
protein expression (insoluble); Trx: Trx-P-CNP fusion protein
expression (soluble); MBP: MBP-P-CNP fusion protein expression
(soluble); TAF: TAF-P-CNP fusion protein expression (insoluble)
(BL21); TAF': TAF-P-CNP fusion protein expression (insoluble)
BL21(DE3), where CNP is Gly-CNP37.
FIG. 2 shows formic acid cleavage of TAF-CNP inclusion bodies. M:
protein marker; 1: Gly-CNP37 positive control; 2: uncleaved TAF-CNP
inclusion bodies; 3: 2% formic acid cleaved TAF-CNP inclusion
bodies, where CNP is Gly-CNP37.
FIGS. 3A-E depict expression of CNP fusion proteins in E. coli. M:
protein marker; Tu: total un-induced cell lysates; Su: un-induced
soluble supernatants; T: total induced cell lysates; S: soluble
supernatants; Cl: CNP22; C: Gly-wtCNP37 ("CNP38"); P: insoluble
pellets. A: KSI: KSI-CNP38(M/N) fusion protein expression
(Insoluble); KSI': KSI-Pro-CNP38 (Pro-Gly-wtCNP37 is designated
"Pro-CNP38") fusion protein expression (Insoluble); Trx:
Trx-Pro-CNP38 fusion expression (Soluble); MBP: MBP-Pro-CNP38
fusion protein expression (Soluble); TAF: TAF-Pro-CNP38 fusion
protein expression (Insoluble) from BL21 cell; TAF': TAF-Pro-CNP38
fusion protein expression (Insoluble) from BL21(DE3) cell. B:
TAF-Pro-CNP37 and BMP-Pro-CNP37 fusion protein expression. C:
BMP-Pro-CNP38 fusion protein and BMP protein expression. D:
TAF-Pro-HSA-CNP ("Pro-GH-KSEVAHRFK-wtCNP27 (SEQ ID NO: 188) is
designated "Pro-HSA-CNP") fusion protein expression. E:
TAF-Pro-CNP38 fusion protein and TAF protein expression.
FIGS. 4A-C depict formic acid cleavage of TAF-Pro-CNP38 inclusion
bodies. A: 50% formic acid cleavage of TAF-Pro-CNP38 inclusion
bodies. M: protein marker; U: un-cleaved TAF-Pro-CNP38 inclusion
bodies; 25.degree. C., 37.degree. C., 42.degree. C., 55.degree. C.:
TAF-Pro-CNP38 inclusion bodies were cleaved in 50% formic acid at
25.degree. C., 37.degree. C., 42.degree. C. or 55.degree. C. for 24
hours. 37.degree. C.-S and 55.degree. C.-S: soluble supernatants
from the cleavage reactions at 37.degree. C. and 55.degree. C.
neutralized with 10N NaOH and centrifuged at 14,000 rpm for 15
minutes. B: 10% and 2% formic acid cleavage of TAF-Pro-CNP38
inclusion bodies. M: protein marker; U: un-cleaved TAF-Pro-CNP38
inclusion bodies; C: formic acid cleaved TAF-Pro-CNP38; S: soluble
supernatant after centrifuged at 14,000 rpm for 5 minutes without
neutralization; P: insoluble pellet after centrifuged at 14,000 rpm
for 5 minutes without neutralization. C: LC/MS analysis of 2% and
10% formic acid cleaved products from TAF-Pro-CNP38 inclusion
bodies.
FIGS. 5A-C depict formic acid cleavage of TAF-Pro-CNP38 inclusion
bodies at different temperature and time of formic acid cleavage.
M: protein marker; C: Gly-wtCNP37 ("CNP38") positive control; U:
un-cleaved TAF-Pro-CNP38 inclusion bodies. A: 2% formic acid
cleaved TAF-Pro-CNP38 at 42.degree. C., 55.degree. C. or 70.degree.
C. for 6, 24 or 48 hours. B: 2% formic acid cleaved TAF-Pro-CNP38
at 55.degree. C., 60.degree. C., 65.degree. C. or 70.degree. C. for
17 or 24 hours. C: LC/MS analysis of 2% formic acid cleaved
products from TAF-Pro-CNP38 inclusion bodies.
FIG. 6 is an SDS-PAGE (Coomassie blue stain) for the expression of
a TAF-CNP34 fusion protein. M: protein marker; C: control
[Gly-CNP37 ("CNP38")]; T: total cell lysates; S: supernatant; TI:
total cell lysates induced; SI: supernatant induced.
FIG. 7 is an SDS-PAGE (Coomassie blue stain) for the expression of
fusion proteins TAF-NL-(C/A & 6D/6E)-Pro-CNP38, TAF(C/A &
10D/10E)-Pro-CNP38, and TAF-Pro-CNP53, where "Pro-CNP38" denotes
Pro-Gly-CNP37. M: protein marker; C: control [Gly-CNP37 ("CNP38")];
T: total cell lysates; TI: total cell lysates induced; S:
supernatant; SI: supernatant induced.
FIG. 8 is an SDS-PAGE (Coomassie blue stain) of the products of
formic acid cleavage of the fusion proteins TAF-CNP34 and
TAF-Pro-CNP53. M: protein marker; P: positive control [Gly-CNP37
("CNP38")]; U: uncleaved; C: cleaved; CS: cleaved supernatant; CP:
cleaved pellet.
FIG. 9 is an LC/MS chromatogram showing the peak for CNP-34 after
formic acid cleavage of TAF-CNP34.
FIG. 10 is an LC/MS chromatogram showing the peak for Pro-CNP53
after formic acid cleavage of TAF-Pro-CNP53.
FIG. 11 is an SDS-PAGE (Coomassie blue stain) for the expression of
fusion proteins TAF(C/A & 4D/4E)-Pro-CNP38 and
TAF(4D/4E)-Pro-CNP38, where "Pro-CNP38" denotes Pro-Gly-CNP37. M:
protein marker; C: control [Gly-CNP37 ("CNP38")]; T: total cell
lysates; TI: total cell lysates induced; S: supernatant; SI:
supernatant induced.
FIG. 12 is an SDS-PAGE (Coomassie blue stain) of the products of
formic acid cleavage of the fusion proteins TAF (4D/4E)-Pro-CNP38
and TAF(C/A & 4D/4E)-Pro-CNP38, where "Pro-CNP38" denotes
Pro-Gly-CNP37. M: protein marker; P: positive control [Gly-CNP37
("CNP38")]; U: uncleaved; C: cleaved; CS: cleaved supernatant; CP:
cleaved pellet.
FIG. 13 is an SDS-PAGE (Coomassie blue stain) of the products of
formic acid cleavage of the fusion proteins TAF-NL-(C/A &
6D/6E)-Pro-CNP38 and TAF(C/A & 10D/10E)-Pro-CNP38, where
"Pro-CNP38" denotes Pro-Gly-CNP37. M: protein marker; P: positive
control [Gly-CNP37 ("CNP38")]; U: uncleaved; C: cleaved; CS:
cleaved supernatant; CP: cleaved pellet.
FIG. 14 is a Western blot, using an anti-CNP antibody, of a
TAF-Pro-CNP38 fusion protein produced in a 10 L fermentation of
BL21(DE3) cells, where the cells were induced at OD.sub.600=64 and
hour 17 and "Pro-CNP38" denotes Pro-Gly-CNP37.
FIG. 15 is an SDS-PAGE (Coomassie blue stain) of eluate fractions
from SP-Sepharose cation-exchange column chromatography of a cruder
Pro-Gly-CNP37 ("Pro-CNP38") product. A: TAF-Pro-CNP38 inclusion
body (IB) in water; B: IB in formate; C: IB in formate,
neutralized; D: neutralized pellet; E: neutralized supernatant; F:
TMAE Hi-CAP load; G: TMAE Hi-CAP flow through/SP-Sepharose load; H:
SP-Sepharose flow through; SP-Sepharose eluate fractions 1-47: 10
uL/lane.
FIG. 16 shows the degree of resistance of N-terminal PEGylated
CNP22 conjugates to neutral endopeptidase (NEP) in vitro.
FIG. 17 depicts the degree of NEP resistance of CNP variants having
an amino acid extension at the N-terminus ["CNP27" is
GANRR-CNP22(K4R) (SEQ ID NO: 36)].
FIG. 18 illustrates the degree of NEP resistance of N-terminal
PEGylated CNP17 and GANRR-CNP22(K4R) ("CNP27") (SEQ ID NO: 36).
FIG. 19 illustrates the degree of NEP resistance of wtCNP22 and CNP
variants Gly-CNP37, GHKSEVAHRFK-wtCNP27 ("CNP27-HSA" in the
figures) (SEQ ID NO: 144) and PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12"
in the figures) (SEQ ID NO: 36).
FIG. 20 shows the ability of CNP variants having an N-terminal
amino acid extension to stimulate cGMP production in NIH3T3 cells
in vitro. The results are relative to the level of cGMP produced in
the presence of 1 uM CNP22. "CNP27" is GANRR-CNP22(K4R) (SEQ ID NO:
36)
FIG. 21 displays the ability of N-terminal PEGylated CNP17 and
GANRR-CNP22(K4R) ("CNP27") (SEQ ID NO: 36) to stimulate cGMP
production in NIH3T3 cells.
FIG. 22 illustrates the effects of N-terminal PEGylation of CNP22
on cGMP production.
FIG. 23 illustrates the cGMP production induced by wtCNP22 and CNP
variants Gly-CNP37, GHKSEVAHRFK-wtCNP27 ("CNP27-HSA") (SEQ ID NO:
144), wtCNP29 and PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12") (SEQ ID
NO: 36) in NIH3T3 cells.
FIGS. 24A and B show that CNP-22 and Pro-Gly-CNP37 ("Pro-CNP38")
stimulated cGMP production through NPR-B with similar dose-response
curves, and to a much greater extent than through NPR-A, and
exhibited a similar profile for NPR-B vs. NPR-C selectivity in in
vitro signaling competition assays.
FIG. 25 demonstrates that exposure of rat chondrosarcoma cells to
CNP22 1 hour once daily or 2 hours once daily has substantially
similar effectiveness in reversing FGF2-induced arrest of
chondrocyte growth as continuous exposure to CNP22.
FIGS. 26A and B show results from a dose response study of CNP22
effects on FGF2-arrested rat chondrosarcoma (RCS) cells.
FIGS. 27A-D show that addition of CNP22 to RCS cells arrested by
FGF2 increases matrix synthesis and partly inhibits FGF2, as
assessed by .sup.35S-sulfate and .sup.3H-Pro incorporation into or
decrease from matrix. Panels A and C, synthesis; B and D,
degradation; A and B, .sup.35S measurement; C and D, .sup.3H
measurement. Statistically significant differences are high-lighted
(ANOVA; *p<0.05, **p<0.01).
FIGS. 28A-C show the levels of aggrecan and fibronectin production
(mRNA, panels A and C, and protein, panel B) in RCS cells cultured
with FGF2 and CNP22.
FIG. 29 shows the efficacy of CNP37 and PEO24-GANRR-CNP22(K4R)
("CNP27-PEO24" in the figures) (SEQ ID NO: 36) in stimulating
longitudinal growth of wild-type femur in an ex vivo mouse organ
model.
FIG. 30 shows longitudinal bone growth of 2-3 day-old wild-type
mouse tibias treated with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) every two days. Results are normalized to
measurements prior to treatment (day 0). Data is represented as
means.+-.SEM (n=8).
FIG. 31 shows longitudinal bone growth of 2-3 day old
achondroplastic FGFR3.sup.ach mouse tibias treated with CNP22,
CNP37 or PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) every two days.
Results are normalized to measurements prior to treatment (day 0).
Data is represented as means.+-.SEM (n=7-8).
FIG. 32 shows longitudinal bone growth of 2-3 day-old wild-type
mouse femurs treated with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) every two days. Results are normalized to
measurements prior to treatment (day 0). Data is represented as
means.+-.SEM (n=8).
FIG. 33 shows longitudinal bone growth of 2-3 day-old FGFR3.sup.ach
mouse femurs treated with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) every two days. Results are normalized to
measurements prior to treatment (day 0). Data is represented as
means.+-.SEM (n=3-7).
FIGS. 34A-I depict CNP37 biodistribution in FGFR3.sup.ach mouse
femurs treated ex vivo every two days. Panels A-C illustrate
distribution in distal femurs, panels D-F illustrate distribution
in articular chondrocytes and panels G-I illustrate distribution in
hypertrophic chondrocytes.
FIGS. 35A-C depict the cellularity of proliferating columns in
growth plates after treatment of wild-type and FGFR3.sup.ach mouse
femurs with CNP22 or CNP37 every two days for 8 days. (A) no
treatment, (B) cell numbers per column after treatment, (C)
morphological studies after treatment. The panels in FIGS. 35C(i)
to C(vi). correspond to the sample order set out in FIG. 35B. Data
is represented as means.+-.SEM (n=4-8).
FIGS. 36A-C depict chondrocyte hypertrophy after ex vivo treatment
of wild-type and FGFR3.sup.ach mouse femurs with CNP22 or CNP37
every two days for 8 days. (A) no treatment, (B) cell size after
treatment, (C) morphological studies after treatment. The panels in
FIGS. 36C(i) to C(vi) correspond to the sample order set out in
FIG. 36B. Data is represented as means.+-.SEM (n32 4-9).
FIGS. 37A-I depict the biodistribution of CNP37 in FGFR3.sup.ach
mouse tibias treated in vivo. Panels A-C show distribution in
distal femurs, panels D-F illustrate distribution in articular
chondrocytes and panels G-I illustrate distribution in hypertrophic
chondrocytes.
FIGS. 38A-C illustrate the in vivo effects of CNP37 on
FGFR3.sup.ach mouse tibia growth plate: (A) total growth plate
thickness, (B) proliferating zone thickness, and (C) hypertrophic
zone thickness. Data is represented as means.+-.SEM (n=7-15).
FIG. 39 shows cGMP levels in conditioned media from wild-type mouse
femurs treated ex vivo with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R)
("CNP27-PEO24") (SEQ ID NO: 36) (p<0.01).
FIG. 40 shows cGMP levels in conditioned media from FGFR3.sup.ach
mouse femurs treated ex vivo with CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) (p<0.01).
FIG. 41 depicts cGMP levels in conditioned media from wild-type
mouse tibias treated ex vivo with CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) (p<0.01).
FIG. 42 shows cGMP levels in conditioned media from FGFR3.sup.ach
mouse tibias treated ex vivo with CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) (p<0.01).
FIG. 43 demonstrates that ex vivo exposure of wild-type and
FGFR3.sup.ach mouse femurs to CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) increased the levels of
cleaved collagen type II in the conditioned media (p<0.05).
FIG. 44 depicts the hypertrophic region of femoral bones isolated
from wild-type mice and FGFR3.sup.Y367C mice (a mouse model of
severe achondroplasia) and treated ex vivo with vehicle or 1 uM
Pro-Gly-CNP37 ("ProCNP38") for 6 days, demonstrating that
Pro-Gly-CNP37 treatment resulted in increase in bone growth and
expansion in the growth plate.
FIG. 45 shows that CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36)
intravenously (i.v.) administered to rats have a much longer
half-life and a much greater bioavailability in the plasma than
CNP22.
FIG. 46 illustrates that PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36)
subcutaneously (s.c.) administered to rats also has a much longer
half-life and a much greater bioavailability in the plasma than
CNP22.
FIG. 47 demonstrates that i.v. administered CNP37 and
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) stimulate a much greater
level of cGMP production in rats than CNP22.
FIG. 48 shows that s.c. administered PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36) and, to a lesser extent, CNP37 are substantially more
effective in stimulating cGMP production in rats than CNP22.
FIG. 49 shows body weight measurements of wild-type mice treated
with Gly-CNP37 or PEO12-GANRR-CNP22(K4R) (SEQ ID NO: 36).
FIG. 50 shows tail length measurements of wild-type mice treated
with Gly-CNP37 or PEO12-GANRR-CNP22(K4R) (SEQ ID NO: 36).
FIG. 51 illustrates the effect of treatment of FGFR3.sup.ach mice
with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) on body
length (p=0.02, 1-tailed t-test, unequal variance).
FIG. 52 shows the effect of CNP22, CNP37 and PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) on tail length in FGFR3.sup.ach mice.
FIGS. 53A and B show the effect of CNP22, CNP37 and
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) on the length of distal long
bones (A, ulna; B, tibia) in FGFR3.sup.ach mice (p<0.01,
one-tailed t-test, unequal variance).
FIGS. 54A and B show the effect of CNP22, CNP37 and
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) on the length of proximal
bones (A, humerus; B, femur) in FGFR3.sup.ach mice (p<0.01,
one-tailed t-test, unequal variance).
FIG. 55 illustrates that CNP37 administration corrects rhizomelia
(disproportion of the length of the proximal limbs) as assessed by
the femur:tibia ratio observed in FGFR3.sup.ach mice (p<0.01,
one-tailed t-test, unequal variance).
FIG. 56 shows the effect of CNP22, CNP37 and PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) on head length in FGFR3.sup.ach mice (p<0.01
one-tailed t-test, unequal variance).
FIG. 57 shows that treatment of FGFR3.sup.ach mice with CNP37
increases the size of the external auditory meatus (EAM) (P=0.03,
one-tailed t-test, unequal variance).
FIG. 58 shows the effect of CNP22, CNP37 and PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) on spinal length in achondroplasic mice, expressed
as extension of vertebral bodies (e.g., lumbar vertebra 5).
FIG. 59 shows that treatment of FGFR3.sup.ach mice with CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) results in increased cGMP
plasma levels 15-min post-dose.
FIG. 60 illustrates the serum levels of cleaved collagen type II in
FGFR3.sup.ach mice treated 5 weeks with CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36).
FIG. 61 shows the serum levels of osteocalcin in FGFR3.sup.ach mice
treated 5 weeks with CNP22, CNP37 or PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36).
FIG. 62 shows cGMP plasma levels 15-min post-dose from wild-type
mice treated with Gly-CNP37 or PEO12-GANRR-CNP22(K4R) (SEQ ID NO:
36) (p<0.05).
FIG. 63 shows the serum levels of cleaved collagen type II in
wild-type mice treated 5 weeks with Gly-CNP37 or
PEO12-GANRR-CNP22(K4R) (SEQ ID NO: 36).
FIGS. 64-66 display the levels of cGMP, cleaved collagen type II
and alkaline phosphatase after administration of vehicle or 20
nmol/kg or 70 nmol/kg Pro-Gly-CNP37 ("Pro-CNP38") to wild-type
mice.
FIGS. 67 and 68 depict cleaved collagen type II and total alkaline
phosphatase levels after administration of vehicle or Pro-Gly-CNP37
("Pro-CNP38") under different dosing regimens.
FIG. 69 illustrates the relative increase in body length of
Pro-Gly-CNP37 ("Pro-CNP38") and vehicle treated animals in two
separate studies (S1 and S2) at Day 37 vs Day 1.
FIGS. 70A and B show changes in bone mineral density (A) and bone
mineral content (B) after administration of Pro-Gly-CNP37
("Pro-CNP38").
FIG. 71 shows plasma cGMP levels 15 min after the last subcutaneous
dose of CNP variant, Day 36, where Gly-wtCNP37 is "CNP38",
Pro-Gly-wtCNP37 is "Pro-CNP38", and GHKSEVAHRFK-wtCNP27 (SEQ ID NO:
144) is "HSA-CNP27" in FIGS. 71-73.
FIG. 72 shows serum levels of cleaved collagen type II from mice
treated with Gly-CNP37, Pro-Gly-CNP37 or GHKSEVAHRFK-CNP27 (SEQ ID
NO: 144).
FIG. 73 shows serum levels of alkaline phosphatase from mice
treated with Gly-CNP37, Pro-Gly-CNP37 or GHKSEVAHRFK-CNP27 (SEQ ID
NO: 144).
FIGS. 74A and B depict desensitization of the cGMP response after
acute (A) or chronic (B) treatment with 1 uM Gly-CNP37.
FIG. 75A demonstrates that daily treatment of wild-type mice with
200 nmol/kg of Pro-Gly-CNP37 ("Pro-CNP38") for 8 days did not
desensitize the cGMP response. FIG. 75B shows that treatment of the
mice with 200 nmol/kg of Pro-Gly-CNP37 once a day for two
consecutive days potentiated the cGMP response.
FIGS. 76A-D show that treatment of wild-type mice with 200 nmol/kg
of Gly-CNP37 stimulated cGMP secretion in distal femurs (cartilage
and bone) (A), femoral cortices (bone) (B), ear pinna (cartilage)
(C), and kidney (D). FIGS. 76E-H show that liver (E), heart (F),
lung (G) and brain (H) tissues did not exhibit appreciable cGMP
secretion in response to Gly-CNP37 relative to vehicle control at
the studied time points.
FIGS. 77-82 show results from an on-going study in normal juvenile
cynomolgus monkeys subcutaneously injected daily with vehicle or 10
or 36 ug/kg of Pro-Gly-CNP37. Both doses of Pro-Gly-CNP37 have
increased growth plate width (FIG. 77), increased right and left
tibia lengths (FIGS. 78A and B), increased leg length (FIG. 79),
increased arm length (FIG. 80), increased body length (FIG. 81),
and increased the serum level of alkaline phosphatase (FIG.
82).
FIG. 83 depicts the observed plot of degradation rate constant
(K.sub.obs) vs. pH at pH 3-8 and 5.degree. C., 25.degree. C. and
40.degree. C. for Gly-CNP37 formulations.
DETAILED DESCRIPTION OF THE DISCLOSURE
The present disclosure relates to novel variants of CNP having
reduced affinity to NEP and/or NPR-C, and reduced susceptibility to
cleavage by NEP and/or clearance by NPR-C, pharmaceutical
compositions comprising such CNP variants, and methods of using
such
CNP variants to treat disorders responsive to CNP, including but
not limited to bone-related disorders such as achondroplasia and
disorders associated with vascular smooth muscle cells and
tissues.
A. Definitions
Unless otherwise stated, the following terms used in this
application, including the specification and claims, have the
definitions given below.
As used in the specification and the appended claims, the
indefinite articles "a" and "an" and the definite article "the"
include plural as well as singular referents unless the context
clearly dictates otherwise.
The term "about" or "approximately" means an acceptable error for a
particular value as determined by one of ordinary skill in the art,
which depends in part on how the value is measured or determined.
In certain embodiments, the term "about" or "approximately" means
within 1, 2, 3, or 4 standard deviations. In certain embodiments,
the term "about" or "approximately" means within 30%, 25%, 20%,
15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.05% of a
given value or range. Whenever the term "about" or "approximately"
precedes the first numerical value in a series of two or more
numerical values, it is understood that the term "about" or
"approximately" applies to each one of the numerical values in that
series.
The terms "ambient temperature" and "room temperature" are used
interchangeably herein and refer to the temperature of the
surrounding environment (e.g., the room in which a reaction is
conducted or a composition is stored). In certain embodiments,
ambient temperature or room temperature is a range from about
15.degree. C. to about 28.degree. C., or from about 15.degree. C.
to about 25.degree. C., or from about 20.degree. C. to about
28.degree. C., or from about 20.degree. C. to about 25.degree. C.,
or from about 22.degree. C. to about 28.degree. C., or from about
22.degree. C. to about 25.degree. C. In other embodiments, ambient
temperature or room temperature is about 15.degree. C., 16.degree.
C., 17.degree. C., 18.degree. C., 19.degree. C., 20.degree. C.,
21.degree. C., 22.degree. C., 23.degree. C., 24.degree. C.,
25.degree. C., 26.degree. C., 27.degree. C. or 28.degree. C.
Definition of standard chemistry terms may be found in reference
works, including Carey and Sundberg, Advanced Organic Chemistry,
3.sup.rd Edition, Vols. A and B (Plenum Press, New York 1992). The
practice of the present disclosure may employ, unless otherwise
indicated, certain conventional methods of synthetic organic
chemistry, mass spectrometry, preparative and analytical
chromatography, protein chemistry, biochemistry, recombinant DNA
technology and pharmacology, within the skill of the art. See,
e.g., T. E. Creighton, Proteins: Structures and Molecular
Properties (W.H. Freeman and Company, 1993); A. L. Lehninger,
Biochemistry (Worth Publishers, Inc., 4.sup.th Edition, 2004);
Sambrook, et al., Molecular Cloning: A Laboratory Manual (2.sup.nd
Edition, 1989); Methods In Enzymology (S. Colowick and N. Kaplan
eds., Academic Press, Inc.); Remington's Pharmaceutical Sciences,
18.sup.th Edition (Easton, Pa.: Mack Publishing Company, 1990).
All publications, patents and patent applications cited herein,
whether supra or infra, are hereby incorporated by reference in
their entirety.
The following amino acid abbreviations are used throughout the
text:
TABLE-US-00009 Alanine: Ala (A) Arginine: Arg (R) Asparagine: Asn
(N) Aspartic acid: Asp (D) Cysteine: Cys (C) Glutamine: Gln (Q)
Glutamic acid: Glu (E) Glycine: Gly (G) Histidine: His (H)
Isoleucine: Ile (I) Leucine: Leu (L) Lysine: Lys (K) Methionine:
Met (M) Phenylalanine: Phe (F) Proline: Pro (P) Serine: Ser (S)
Threonine: Thr (T) Tryptophan: Trp (W) Tyrosine: Tyr (Y) Valine:
Val (V)
"Polypeptide" and "protein" refer to a polymer composed of amino
acid residues, related naturally occurring structural variants, and
synthetic non-naturally occurring analogs thereof, linked via
peptide bonds or peptide bond isosteres. Synthetic polypeptides can
be synthesized, for example, using an automated polypeptide
synthesizer. The terms "polypeptide" and "protein" are not limited
to a minimum length of the product. The term "protein" typically
refers to large polypeptides. The term "peptide" typically refers
to short polypeptides. Thus, peptides, oligopeptides, dimers,
multimers, and the like, are included within the definition. Both
full-length proteins and fragments thereof are encompassed by the
definition. The terms "polypeptide" and "protein" also include
postexpression modifications of the polypeptide or protein, for
example, glycosylation, acetylation, phosphorylation and the like.
Furthermore, for purposes of the present disclosure, a
"polypeptide" can include "modifications," such as deletions,
additions, substitutions (which may be conservative in nature or
may include substitutions with any of the 20 amino acids that are
commonly present in human proteins, or any other naturally or
non-naturally-occurring or atypical amino acids), and chemical
modifications (e.g., addition of or substitution with
peptidomimetics), to the native sequence. These modifications may
be deliberate, as through site-directed mutagenesis, or through
chemical modification of amino acids to remove or attach chemical
moieties, or may be accidental, such as through mutations arising
with hosts that produce the proteins or through errors due to PCR
amplification.
Conventional notation is used herein to portray polypeptide
sequences: the left-hand end of a polypeptide sequence is the
amino-terminus; the right-hand end of a polypeptide sequence is the
carboxyl-terminus.
"Conservative substitution" refers to substitution of an amino acid
in a polypeptide with a functionally, structurally or chemically
similar natural or unnatural amino acid. In one embodiment, the
following groups each contain natural amino acids that are
conservative substitutions for one another:
(1), Alanine (A) Serine (S), Threonine (T);
(2) Aspartic acid (D), Glutamic acid (E);
(3) Asparagine (N), Glutamine (Q);
(4) Arginine (R), Lysine (K);
(5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V);
and
(6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W).
In another embodiment, the following groups each contain natural
amino acids that are conservative substitutions for one
another:
(1) Glycine (G), Alanine (A);
(2) Aspartic acid (D), Glutamic acid (E);
(3) Asparagine (N), Glutamine (Q);
(4) Arginine (R), Lysine (K);
(5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V),
Alanine (A);
(6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); and
(7) Serine (S), Threonine (T), Cysteine (C).
In a further embodiment, amino acids may be grouped as set out
below.
(1) hydrophobic: Met, Ala, Val, Leu, Ile, Phe, Trp;
(2) neutral hydrophilic: Cys, Ser, Thr, Asn, Gln;
(3) acidic: Asp, Glu;
(4) basic: His, Lys, Arg;
(5) residues that influence backbone orientation: Gly, Pro; and
(6) aromatic: Trp, Tyr, Phe, His.
In one embodiment, the peptides or polypeptides described herein
are generated via recombinant means, using a polynucleotide
encoding a CNP variant. The disclosure thus encompasses
polynucleotides encoding any of the CNP variants described herein,
host cells or vectors comprising such polynucleotides, optionally
linked to expression control sequences, and methods of using such
polynucleotides, vectors or host cells to produce CNP variants of
the disclosure. CNP variants expressed by such polynucleotides may
be produced by methods including growing host cells in culture
medium under conditions suitable for expression of the
polynucleotide encoding a CNP variant, and isolating the expression
product from the host cells or culture medium. Actual expression
products may vary slightly from the encoded protein product
depending on any post-translational processing.
"Polynucleotide" refers to a polymer composed of nucleotide units.
Polynucleotides include naturally occurring nucleic acids, such as
deoxyribonucleic acid ("DNA") and ribonucleic acid ("RNA") as well
as nucleic acid analogs. The term "nucleic acid" typically refers
to large polynucleotides. The term "oligonucleotide" typically
refers to short polynucleotides, generally no greater than about 50
nucleotides. It will be understood that when a nucleotide sequence
is represented by a DNA sequence (i.e., A, T, G, C), the nucleotide
sequence also encompasses an RNA sequence (i.e., A, U, G, C) in
which "U" replaces "T." "cDNA" refers to a DNA that is
complementary or identical to an mRNA, in either single stranded or
double stranded form.
"Expression control sequence" refers to a nucleotide sequence that
regulates the expression of a nucleotide sequence operatively
linked thereto. "Operatively linked" refers to a functional
relationship between two parts in which the activity of one part
(e.g., the ability to regulate transcription) results in an action
on the other part (e.g., transcription of the sequence). Expression
control sequences can include, for example and without limitation,
sequences of promoters (e.g., inducible or constitutive),
enhancers, transcription terminators, a start codon (i.e., ATG),
splicing signals for introns, and stop codons.
"Recombinant polynucleotide" refers to a polynucleotide having
sequences that are not naturally joined together. An amplified or
assembled recombinant polynucleotide may be included in a suitable
vector, and the vector can be used to transform a suitable host
cell. A host cell that comprises the recombinant polynucleotide is
referred to as a "recombinant host cell." The gene is then
expressed in the recombinant host cell to produce, e.g., a
"recombinant polypeptide." A recombinant polynucleotide may serve a
non-coding function (e.g., promoter, origin of replication,
ribosome-binding site, etc.) as well.
"Chimera" as used herein refers to a polynucleotide or polypeptide
comprising at least two heterologous polynucleotide or polypeptide
sequences (i.e. derived from different sources or not associated
with each other as a naturally-occurring sequence) which are
directly or indirectly attached or linked together using techniques
commonly known in the art, e.g., recombinant expression or chemical
crosslinking. In one embodiment, the heterologous sequence can
comprise a protein or peptide directly or indirectly linked to a
CNP peptide or variant, including proteins or peptides which are
cleavable from the CNP peptide or variant. In a related embodiment,
CNP variants are chimera as described herein.
In certain embodiments, chimeras include CNP fusion proteins
comprising a cleavable carrier protein or peptide tag. The term
"cleavable carrier protein" or "cleavable peptide tag" refers to a
peptide or polypeptide sequence that may be fused, directly or
indirectly via a linker, to a heterologous polypeptide sequence,
and is removable from the heterologous sequence using an agent that
cleaves or separates the cleavable peptide or polypeptide from the
heterologous polypeptide or protein. In some embodiments, the
cleavable carrier protein or peptide tag improves generation,
purification and/or detection of the fusion protein or the
heterologous polypeptide. Exemplary cleavable carrier proteins and
peptide tags include, but are not limited to, human transcription
factor TAF12 (TAF12), ketosteroid isomerase (KSI), maltose-binding
protein (MBP), .beta.-galactosidase (.beta.-Gal),
glutathione-S-transferase (GST), thioredoxin (Trx), chitin-binding
domain (CBD), BMP-2 mutation (BMPM), SUMO, CAT, TrpE,
staphylococcal protein A, streptococcal proteins, starch-binding
protein, cellulose-binding domain of endoglucanase A,
cellulose-binding domain of exoglucanase Cex, biotin-binding
domain, recA, Flag, c-Myc, poly(His), poly (Arg), poly(Asp),
poly(Gln), poly(Phe), poly(Cys), green fluorescent protein, red
fluorescent protein, yellow fluorescent protein, cyan fluorescent
protein, biotin, avidin, streptavidin, antibody epitopes, and
fragments thereof.
A "cleaving agent" is an agent that is useful to cleave or
separate, e.g., a cleavable peptide or polypeptide from a
heterologous polypeptide or protein. Exemplary cleaving agents
include, but are not limited to, palladium, cyanogen bromide
(CNBr), formic acid, hydroxylamine, clostripain, thrombin,
chymotrypsin, trypsin, trypsin-like proteases, carboxypeptidase,
enterokinase (enteropeptidase), Kex 2 protease, Omp T protease,
Factor Xa protease, subtilisin, proTEV, SUMO protease, V8 protease,
HIV protease, rhinovirus protease, furilisin protease, IgA
proteases, human Pace protease, collagenase, Nia protease,
poliovirus 2Apro protease, poliovirus 3C protease, genenase, furin,
elastase, Proteinase K, pepsin, rennin (chymosin), microbial
aspartic proteases, papain, calpain, chymopapain, ficin (ficain),
bromelain (bromelase), cathespisin B, caspases, thermolysin,
Endoprotease Arg-C, Endoprotease Glu-C, Endoprotease Lys-C,
kallikrein, and plasmin.
The terms "identical" and percent "identity", in the context of two
or more polynucleotide or polypeptide sequences, refer to two or
more sequences or subsequences that are the same or have a
specified percentage of nucleotides or amino acid residues that are
the same, when compared and aligned for maximum correspondence, as
measured using one of the following sequence comparison algorithms
or by visual inspection.
The phrase "substantially homologous" or "substantially identical",
in the context of two nucleic acids or polypeptides, generally
refers to two or more sequences or subsequences that have at least
40%, 50%, 60%, 70%, 80%, 90%, 95%, or 98% nucleotide or amino acid
residue identity, when compared and aligned for maximum
correspondence, as measured using one of the following sequence
comparison algorithms or by visual inspection. In certain
embodiments, the substantial homology or identity exists over
regions of the sequences that are at least about 25, 50, 100 or 150
residues in length. In another embodiment, the sequences are
substantially homologous or identical over the entire length of
either or both comparison biopolymers.
For sequence comparison, typically one sequence acts as a reference
sequence, to which test sequences are compared. When using a
sequence comparison algorithm, test and reference sequences are
inputted into a computer, subsequence coordinates are designated,
if necessary, and sequence algorithm program parameters are
designated. The sequence comparison algorithm then calculates the
percent sequence identity for the test sequence(s) relative to the
reference sequence, based on the designated program parameters.
Optimal alignment of sequences for comparison can be conducted,
e.g., by the local homology algorithm of Smith & Waterman, Adv.
Appl. Math., 2: 482 (1981), by the homology alignment algorithm of
Needleman & Wunsch, J. Mol. Biol., 48: 443 (1970), by the
search for similarity method of Pearson & Lipman, Proc. Natl.
Acad. Sci. USA, 85: 2444 (1988), by computerized implementations of
these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin
Genetics Software Package, Genetics Computer Group, 575 Science
Dr., Madison, Wis.), or by visual inspection. One example of a
useful algorithm is PILEUP, which uses a simplification of the
progressive alignment method of Feng & Doolittle, J. Mol.
Evol., 35: 351-360 (1987) and is similar to the method described by
Higgins & Sharp, CABIOS, 5: 151-153 (1989). Another algorithm
useful for generating multiple alignments of sequences is Clustal W
(Thompson et al., Nucleic Acids Research, 22: 4673-4680 (1994)). An
example of an algorithm that is suitable for determining percent
sequence identity and sequence similarity is the BLAST algorithm
(Altschul et al., J. Mol. Biol., 215: 403-410 (1990); Henikoff
& Henikoff, Proc. Natl. Acad. Sci. USA, 89: 10915 (1989);
Karlin & Altschul, Proc. Natl. Acad. Sci. USA, 90: 5873-5787
(1993)). Software for performing BLAST analyses is publicly
available through the National Center for Biotechnology
Information.
A further indication that two nucleic acid sequences or
polypeptides are substantially homologous or identical is that the
polypeptide encoded by the first nucleic acid is immunologically
cross reactive with the polypeptide encoded by the second nucleic
acid, as described below. Thus, a polypeptide is typically
substantially identical to a second polypeptide, for example, where
the two polypeptides differ only by conservative substitutions.
Another indication that two nucleic acid sequences are
substantially identical is that the two molecules hybridize to each
other under stringent conditions, as described herein.
"Substantially pure" or "isolated" means an object species is the
predominant species present (i.e., on a molar basis, more abundant
than any other individual macromolecular species in the
composition), and a substantially purified fraction is a
composition wherein the object species comprises at least about 50%
(on a molar basis) of all macromolecular species present. In one
embodiment, a substantially pure composition means that the species
of interest comprises at least about 70%, 75%, 80%, 85%, 90%, 95%,
98% or more of the macromolecular species present in the
composition on a molar or weight basis. The object species is
purified to essential homogeneity (contaminant species cannot be
detected in the composition by conventional detection methods) if
the composition consists essentially of a single macromolecular
species. Solvent species, small molecules (<500 Daltons),
stabilizers (e.g., BSA), and elemental ion species are not
considered macromolecular species for purposes of this definition.
In an embodiment, the compounds of the disclosure are substantially
pure or isolated. In another embodiment, the compounds of the
disclosure are substantially pure or isolated with respect to the
macromolecular starting materials used in their production. In yet
another embodiment, the pharmaceutical compositions of the
disclosure comprise a substantially pure or isolated CNP variant
admixed with one or more pharmaceutically acceptable excipients,
carriers or diluents, and optionally with another biologically
active agent.
"Naturally occurring" as applied to an object refers to the fact
that the object can be found in nature. For example, a polypeptide
or polynucleotide sequence that is present in an organism
(including viruses) and which has not been intentionally modified
by man in the laboratory is naturally occurring. In one embodiment,
a "naturally occurring" substance is of human origin.
"Wild-type" (wt) is a term referring to the natural form, including
sequence, of a polynucleotide, polypeptide or protein in a species.
A wild-type form is distinguished from a mutant form of a
polynucleotide, polypeptide or protein arising from genetic
mutation(s).
In one embodiment, a first polypeptide that is an "analog" or
"variant" or "derivative" of a second polypeptide is a polypeptide
having at least about 50%, 60% or 70% sequence homology, but less
than 100% sequence homology, with the second polypeptide. Such
analogs, variants or derivatives may be comprised of non-naturally
occurring amino acid residues, including without limitation,
homoarginine, ornithine, penicillamine, and norvaline, as well as
naturally occurring amino acid residues. Such analogs, variants or
derivatives may also be composed of one or a plurality of D-amino
acid residues, and may also contain peptidomimetics or peptide bond
isosteres such as non-peptide linkages between two or more amino
acid or peptidomimetic residues. In another embodiment, a first
polypeptide is an "analog", "variant" or "derivative" of a second
polypeptide if the first polypeptide is not a known cleavage
product of the second polypeptide or is not a known precursor of
the second polypeptide, even if the first polypeptide has 100%
sequence homology to the second polypeptide or has a wild-type
sequence.
In an embodiment, the term "derived from" as used herein refers to
a polypeptide or peptide sequence that is based on a wild type or
naturally occurring polypeptide or peptide sequence and can have
one or more deletions, additions, and/or substitutions with natural
amino acids, unnatural amino acids or peptidomimetics. In one
embodiment, the derivative sequence shares at least about 40%, 50%,
60% or 70%, but less than 100%, sequence similarity to the
wild-type or naturally occurring sequence. In another embodiment,
the derivative may be a fragment of a polypeptide, wherein the
fragment is substantially homologous (e.g., at least about 70%,
75%, 80%, 85%, 90%, or 95% homologous) to the wild-type polypeptide
over a length of at least about 5, 10, 15, 20, 25, 30, 35, 40, 45
or 50 amino acids. In still another embodiment, a polypeptide is
"derived from" a wild-type polypeptide if it has a moiety (e.g., a
polymer such as, e.g., PEG) directly or indirectly attached to it
which is not present on the wild-type polypeptide, even if both
polypeptides share 100% homology in their amino acid sequence.
As used herein, an "NPPC-derived" polypeptide refers to a
polypeptide derived from the natriuretic peptide precursor C(NPPC)
polypeptide, which is a single chain 126-amino acid pre-pro
polypeptide, and which upon cleavage ultimately results in wtCNP22.
Removal of the signal peptide from NPPC yields pro-CNP, and further
cleavage by the endoprotease furin generates an active 53-amino
acid peptide (CNP-53), which is secreted and cleaved again by an
unknown enzyme to produce the mature 22-amino acid peptide (CNP, or
CNP-22). Therefore, CNP22 itself is an "NPPC-derived" polypeptide
by virtue of being derived from NPPC. In one embodiment, an
NPPC-derived polypeptide is at least about 40%, 50%, 60%, 70%, 75%,
80%, 85%, 90%, or 95% homologous to the wild type NPPC over the
same number of amino acid residues. It is further contemplated that
an NPPC-derived peptide may comprise from about 1 to about 53, or 1
to 37, or 1 to 35, or 1 to 31, or 1 to 27, or 1 to 22, or to 35, or
about 15 to about 37 residues of the NPPC polypeptide. In one
embodiment, an NPPC-derived peptide may comprise a sequence of 1,
2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20,
21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37,
38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, or 53
amino acids derived from the NPPC polypeptide.
The term "effective amount" means a dosage sufficient to produce a
desired result on a health condition, pathology, or disease of a
subject or for a diagnostic purpose. The desired result may
comprise a subjective or objective improvement in the recipient of
the dosage. "Therapeutically effective amount" refers to that
amount of an agent effective to produce the intended beneficial
effect on health. An appropriate "effective" amount in any
individual case may be determined by one of ordinary skill in the
art using routine experimentation. It will be understood that the
specific dose level and frequency of dosage for any particular
patient may be varied and will depend upon a variety of factors,
including the activity of the specific compound employed; the
bioavailability, metabolic stability, rate of excretion and length
of action of that compound; the mode and time of administration of
the compound; the age, body weight, general health, sex, and diet
of the patient; and the severity of the particular condition.
"Treatment" refers to prophylactic treatment or therapeutic
treatment or diagnostic treatment. In certain embodiments,
"treatment" refers to administration of a compound or composition
to a subject for therapeutic, prophylactic or diagnostic
purposes.
A "prophylactic" treatment is a treatment administered to a subject
who does not exhibit signs of a disease or exhibits only early
signs of the disease, for the purpose of decreasing the risk of
developing pathology. The compounds or compositions of the
disclosure may be given as a prophylactic treatment to reduce the
likelihood of developing a pathology or to minimize the severity of
the pathology, if developed.
A "therapeutic" treatment is a treatment administered to a subject
who exhibits signs or symptoms of pathology for the purpose of
diminishing or eliminating those signs or symptoms. The signs or
symptoms may be biochemical, cellular, histological, functional or
physical, subjective or objective. The compounds of the disclosure
may also be given as a therapeutic treatment or for diagnosis.
"Diagnostic" means identifying the presence, extent and/or nature
of a pathologic condition. Diagnostic methods differ in their
specificity and selectivity. While a particular diagnostic method
may not provide a definitive diagnosis of a condition, it suffices
if the method provides a positive indication that aids in
diagnosis.
"Bone- or cartilage-associated biomarker" or "bone- or
cartilage-associated marker" refers to a growth factor, enzyme,
protein, or other detectable biological substance or moiety whose
level is increased or decreased in association with, e.g.,
cartilage turnover, cartilage formation, cartilage growth, bone
resorption, bone formation, bone growth, or combinations thereof.
Such biomarkers may be measured before, during and/or after
administration of a CNP variant as described herein. Exemplary
bone- or cartilage-associated biomarkers include, but are not
limited to, CNP, cGMP, propeptides of collagen type II and
fragments thereof, collagen type II and fragments thereof,
propeptides of collagen type I and fragments thereof, collagen type
1 and fragments thereof, osteocalcin, proliferating cell nuclear
antigen (PCNA), aggrecan chondroitin sulfate, and alkaline
phosphatase. Cartilage- and bone-associated biomarkers can be
measured in any appropriate biological sample, including but not
limited to tissues, blood, serum, plasma, cerebrospinal fluid,
synovial fluid and urine. In some embodiments, the biomarkers are
measured in blood, plasma or serum from animals undergoing
efficacy/pharmacodynamic in vivo studies and/or from the
conditioned media of ex vivo studies.
In certain embodiments, the level of at least one bone- or
cartilage-associated biomarker is measured and the amount or
frequency of administration of CNP variant administered to a
subject can be adjusted according to the level of the biomarker
measured. In some embodiments, the level of biomarker is "below a
target level" or "above a target level." A target level of a
biomarker is a level or range of levels of the biomarker at which a
therapeutic effect is observed in the subject receiving the CNP
variant. In certain embodiments, the target level of a biomarker
for a subject having a CNP-responsive disorder or condition is the
level or range of levels of the biomarker observed in a normal,
non-affected subject. In other embodiments, to indicate a
therapeutic effect, the target level of a biomarker need not be
equivalent to the level or range of levels of the biomarker
observed in a normal subject, but can be within, e.g., 100%, 90%,
80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% or 5% of the "normal" level
or range of levels of the biomarker observed in a non-affected
subject.
For example, if the level of a biomarker increases in association
with bone or cartilage formation or growth, the target level of the
biomarker indicating a therapeutic effect may be higher than the
level of the biomarker in patients suffering from a CNP-responsive
disorder who have not been administered a CNP variant, and may
optionally be lower than the "normal" level(s), at about the
"normal" level(s), or above the "normal" level(s) of the biomarker
in subjects not suffering from that disorder. In one embodiment, if
the level of a biomarker is below a target level, it indicates an
inadequate therapeutic effect, which may require an increase in the
amount or frequency of administration of CNP variant administered.
In a related embodiment, if the biomarker is above a target level,
it indicates that more CNP variant than necessary has been
administered, which may require a decrease in the amount or
frequency of administration of the CNP variant administered.
As another example, if the level of a biomarker decreases in
association with bone or cartilage formation or growth, the target
level of the biomarker indicating a therapeutic effect may be lower
than the level of the biomarker in patients suffering from a
CNP-responsive disorder who have not been administered a CNP
variant, and may optionally be higher than the "normal" level(s),
at about the "normal" level(s), or below the "normal" level(s) of
the biomarker in subjects not suffering from that disorder. In such
a case, the converse of the above adjustments in CNP variant amount
and frequency of administration may apply.
"Pharmaceutical composition" refers to a composition suitable for
pharmaceutical use in subject animal, including humans and mammals.
A pharmaceutical composition comprises a therapeutically effective
amount of a CNP variant, optionally another biologically active
agent, and optionally a pharmaceutically acceptable excipient,
carrier or diluent. In an embodiment, a pharmaceutical composition
encompasses a composition comprising the active ingredient(s), and
the inert ingredient(s) that make up the carrier, as well as any
product that results, directly or indirectly, from combination,
complexation or aggregation of any two or more of the ingredients,
or from dissociation of one or more of the ingredients, or from
other types of reactions or interactions of one or more of the
ingredients. Accordingly, the pharmaceutical compositions of the
present disclosure encompass any composition made by admixing a
compound of the disclosure and a pharmaceutically acceptable
excipient, carrier or diluent.
"Pharmaceutically acceptable carrier" refers to any of the standard
pharmaceutical carriers, buffers, and the like, such as a phosphate
buffered saline solution, 5% aqueous solution of dextrose, and
emulsions (e.g., an oil/water or water/oil emulsion). Non-limiting
examples of excipients include adjuvants, binders, fillers,
diluents, disintegrants, emulsifying agents, wetting agents,
lubricants, glidants, sweetening agents, flavoring agents, and
coloring agents. Suitable pharmaceutical carriers, excipients and
diluents are described in Remington's Pharmaceutical Sciences, 19th
Ed. (Mack Publishing Co., Easton, 1995). Preferred pharmaceutical
carriers depend upon the intended mode of administration of the
active agent. Typical modes of administration include enteral
(e.g., oral) or parenteral (e.g., subcutaneous, intramuscular,
intravenous or intraperitoneal injection; or topical, transdermal,
or transmucosal administration).
A "pharmaceutically acceptable salt" is a salt that can be
formulated into a compound for pharmaceutical use, including but
not limited to metal salts (e.g., sodium, potassium, magnesium,
calcium, etc.) and salts of ammonia or organic amines.
By "pharmaceutically acceptable" or "pharmacologically acceptable"
is meant a material that is not biologically or otherwise
undesirable, i.e., the material may be administered to an
individual without causing any undesirable biological effects or
without interacting in a deleterious manner with any of the
components of the composition in which it is contained or with any
components present on or in the body of the individual.
The term "unit dosage form" refers to physically discrete units
suitable as unitary dosages for human and animal subjects, each
unit containing a predetermined quantity of a compound of the
disclosure calculated in an amount sufficient to produce the
desired effect, optionally in association with a pharmaceutically
acceptable excipient, diluent, carrier or vehicle. The
specifications for the novel unit dosage forms of the present
disclosure depend on the particular compound employed and the
effect to be achieved, and the pharmacodynamics associated with
each compound in the host.
"Physiological conditions" refer to conditions in the body of an
animal (e.g., a human). Physiological conditions include, but are
not limited to, body temperature and an aqueous environment of
physiologic ionic strength, pH and enzymes. Physiological
conditions also encompass conditions in the body of a particular
subject which differ from the "normal" conditions present in the
majority of subjects, e.g., which differ from the normal human body
temperature of approximately 37.degree. C. or differ from the
normal human blood pH of approximately 7.4.
By "physiological pH" or a "pH in a physiological range" is meant a
pH in the range of approximately 7.0 to 8.0 inclusive, more
typically in the range of approximately 7.2 to 7.6 inclusive.
As used herein, the term "subject" encompasses mammals and
non-mammals. Examples of mammals include, but are not limited to,
any member of the mammalian class: humans, non-human primates such
as chimpanzees, and other apes and monkey species; farm animals
such as cattle, horses, sheep, goats, swine; domestic animals such
as rabbits, dogs, and cats; laboratory animals including rodents,
such as rats, mice and guinea pigs, and the like. Examples of
non-mammals include, but are not limited to, birds, fish, and the
like. The term does not denote a particular age or gender.
The terms "polyethylene glycol", "PEG", "polyethylene oxide" and
"PEO" are used interchangeably herein unless indicated otherwise. A
CNP peptide (CNP22 or a variant thereof) conjugated via an amino
group to a "PEOn" polymer associated with the number n, in general
has the formula:
CH.sub.3--[--O--CH.sub.2CH.sub.2--].sub.n--C(.dbd.O)--NHR, where n
is the number of ethylene oxide units and R denotes the rest of the
peptide. The "PEOn" polymer can optionally have an alkylene group,
(CH.sub.2).sub.m, where m is an integer from 1 to 5, between the
carbonyl carbon and the repeating ethylene oxide units. Such a
"PEOn" (e.g., PEO12 or PEO24) polymer is monodispersed, i.e., is a
single discrete polymer of a particular molecular weight.
Similarly, a CNP peptide conjugated via an amino group to a "PEGnK"
polymer associated with the number nK, in general has the formula:
CH.sub.3--[--O--CH.sub.2CH.sub.2--].sub.p--C(.dbd.O)--NHR, where p
is an integer greater than 1. The "PEGnK" polymer also can
optionally have an alkylene group, (CH.sub.2).sub.m, where m is an
integer from 1 to 5, between the carbonyl carbon and the repeating
ethylene oxide units. However, such a "PEGnK" (e.g., PEG1K, PEG2K,
PEG5K or PEG20K) polymer is polydispersed, i.e., contains a mixture
of polymers having a distribution of molecular weights, where the
number nK denotes the polymer number-average molecular weight
(M.sub.n) in kilo Daltons. For example, "PEG2K" conjugated to a CNP
peptide denotes a polydispersed PEG polymer having a polymer
number-average molecular weight of around 2 kDa.
When a range of the mass of a polymer (e.g., PEG) is given (e.g.,
in units of kDa), the range refers to a range of polymer
number-average molecular weights, not to a range of molecular
weights of multiple polymers in a polydispersed mixture, unless
expressly indicated otherwise.
The term "halogen", "halide" or "halo" refers to fluorine,
chlorine, bromine, and/or iodine.
The term "alkyl" refers to a linear or branched saturated
monovalent hydrocarbon radical, wherein the alkyl may optionally be
substituted with one or more substituents Q as described herein. In
certain embodiments, the alkyl is a linear saturated monovalent
hydrocarbon radical that has 1 to 20 (C.sub.1-20), 1 to 15
(C.sub.1-15), 1 to 12 (C.sub.1-12), 1 to 10 (C.sub.1-10), or 1 to 6
(C.sub.1-6) carbon atoms, or a branched saturated monovalent
hydrocarbon radical of 3 to 20 (C.sub.3-20), 3 to 15 (C.sub.3-15),
3 to 12 (C.sub.3-12), 3 to 10 (C.sub.3-10), or 3 to 6 (C.sub.3-6)
carbon atoms. As used herein, linear C.sub.1-6 and branched
C.sub.3-6 alkyl groups are also referred as "lower alkyl." Examples
of alkyl groups include, but are not limited to, methyl, ethyl,
propyl (including all isomeric forms, including n-propyl and
isopropyl), butyl (including all isomeric forms, including n-butyl,
isobutyl, sec-butyl and tert-butyl), pentyl (including all isomeric
forms), and hexyl (including all isomeric forms). For example,
C.sub.1-6 alkyl refers to a linear saturated monovalent hydrocarbon
radical of 1 to 6 carbon atoms or a branched saturated monovalent
hydrocarbon radical of 3 to 6 carbon atoms.
The term "alkoxy" refers to an --O-alkyl group. In certain
embodiments, an alkoxy group may optionally be substituted with one
or more substituents Q as described herein.
The term "haloalkyl" refers to an alkyl group that is substituted
with one or more halide atoms. In certain embodiments, a haloalkyl
group is substituted with one, two, three, four, five or six halide
atoms. In certain embodiments, a haloalkyl group may optionally be
substituted with one or more additional substituents Q as described
herein.
The term "cycloalkyl" refers to a cyclic saturated bridged and/or
non-bridged monovalent hydrocarbon radical, which may be optionally
substituted with one or more substituents Q as described herein. In
certain embodiments, the cycloalkyl has from 3 to 20 (C.sub.3-20),
from 3 to 15 (C.sub.3-15), from 3 to 12 (C.sub.3-12), from 3 to 10
(C.sub.3-10), or from 3 to 7 (C.sub.3-7) carbon atoms. Examples of
cycloalkyl groups include, but are not limited to, cyclopropyl,
cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, decalinyl, and
adamantyl.
The term "heterocyclyl" or "heterocyclic" refers to a monocyclic
non-aromatic ring system or a multicyclic ring system that contains
at least one non-aromatic ring, wherein one or more of the
non-aromatic ring atoms are heteroatoms independently selected from
O, S, or N, and the remaining non-aromatic ring atoms are carbon
atoms. In certain embodiments, the heterocyclyl or heterocyclic
group has from 3 to 20, from 3 to 15, from 3 to 10, from 3 to 8,
from 4 to 7, or from 5 to 6 ring atoms. In certain embodiments, the
heterocyclyl is a monocyclic, bicyclic, tricyclic, or tetracyclic
ring system, which may include a fused or bridged ring system, and
in which the nitrogen or sulfur atoms may be optionally oxidized,
the nitrogen atoms may be optionally quaternized, and some rings
may be partially or fully saturated, or aromatic. The heterocyclyl
may be attached to the main structure at any heteroatom or carbon
atom which results in the creation of a stable compound. Examples
of heterocyclic groups include, but are not limited to, acridinyl,
azepinyl, benzimidazolyl, benzindolyl, benzoisoxazolyl,
benzisoxazinyl, benzodioxanyl, benzodioxolyl, benzofuranonyl,
benzofuranyl, benzonaphthofuranyl, benzopyranonyl, benzopyranyl,
benzotetrahydrofuranyl, benzotetrahydrothienyl, benzothiadiazolyl,
benzothiazolyl, benzothiophenyl, benzotriazolyl, benzothiopyranyl,
benzoxazinyl, benzoxazolyl, benzothiazolyl, .beta.-carbolinyl,
carbazolyl, chromanyl, chromonyl, cinnolinyl, coumarinyl,
decahydroisoquinolinyl, dibenzofuranyl, dihydrobenzisothiazinyl,
dihydrobenzisoxazinyl, dihydrofuryl, dihydropyranyl, dioxolanyl,
dihydropyrazinyl, dihydropyridinyl, dihydropyrazolyl,
dihydropyrimidinyl, dihydropyrrolyl, dioxolanyl, 1,4-dithianyl,
furanonyl, furanyl, imidazolidinyl, imidazolinyl, imidazolyl,
imidazopyridinyl, imidazothiazolyl, indazolyl, indolinyl,
indolizinyl, indolyl, isobenzotetrahydrofuranyl,
isobenzotetrahydrothienyl, isobenzothienyl, isochromanyl,
isocoumarinyl, isoindolinyl, isoindolyl, isoquinolinyl,
isothiazolidinyl, isothiazolyl, isoxazolidinyl, isoxazolyl,
morpholinyl, naphthyridinyl, octahydroindolyl, octahydroisoindolyl,
oxadiazolyl, oxazolidinonyl, oxazolidinyl, oxazolopyridinyl,
oxazolyl, oxiranyl, perimidinyl, phenanthridinyl, phenathrolinyl,
phenarsazinyl, phenazinyl, phenothiazinyl, phenoxazinyl,
phthalazinyl, piperazinyl, piperidinyl, 4-piperidonyl, pteridinyl,
purinyl, pyrazinyl, pyrazolidinyl, pyrazolyl, pyridazinyl,
pyridinyl, pyridopyridinyl, pyrimidinyl, pyrrolidinyl, pyrrolinyl,
pyrrolyl, quinazolinyl, quinolinyl, quinoxalinyl, quinuclidinyl,
tetrahydrofuryl, tetrahydrofuranyl, tetrahydroisoquinolinyl,
tetrahydropyranyl, tetrahydrothienyl, tetrazolyl,
thiadiazolopyrimidinyl, thiadiazolyl, thiamorpholinyl,
thiazolidinyl, thiazolyl, thienyl, triazinyl, triazolyl, and
1,3,5-trithianyl. In certain embodiments, a heterocyclic group may
optionally be substituted with one or more substituents Q as
described herein.
The term "aryl" refers to a monocyclic aromatic group or a
multicyclic monovalent aromatic group that contain at least one
aromatic hydrocarbon ring. In certain embodiments, the aryl has
from 6 to 20 (C.sub.6-20), from 6 to 15 (C.sub.6-15), or from 6 to
10 (C.sub.6-10) ring atoms. Examples of aryl groups include, but
are not limited to, phenyl, naphthyl, fluorenyl, azulenyl, anthryl,
phenanthryl, pyrenyl, biphenyl, and terphenyl. Aryl also refers to
bicyclic or tricyclic carbon rings, where at least one of the rings
is aromatic and the others may be saturated, partially unsaturated,
or aromatic, for example, dihydronaphthyl, indenyl, indanyl, and
tetrahydronaphthyl (tetralinyl). In certain embodiments, an aryl
group may optionally be substituted with one or more substituents Q
as described herein.
The term "heteroaryl" refers to a monocyclic aromatic group or a
multicyclic aromatic group that contain at least one aromatic ring,
wherein at least one aromatic ring contains one or more heteroatoms
independently selected from O, S, and N. Each ring of a heteroaryl
group can contain one or two O atoms, one or two S atoms, and/or
one to four N atoms, provided that the total number of heteroatoms
in each ring is four or less and each ring contains at least one
carbon atom. The heteroaryl may be attached to the main structure
at any heteroatom or carbon atom which results in the creation of a
stable compound. In certain embodiments, the heteroaryl has from 5
to 20, from 5 to 15, or from 5 to 10 ring atoms. Examples of
monocyclic heteroaryl groups include, but are not limited to,
pyrrolyl, pyrazolyl, pyrazolinyl, imidazolyl, oxazolyl, isoxazolyl,
thiazolyl, thiadiazolyl, isothiazolyl, furanyl, thienyl,
oxadiazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, and
triazinyl. Examples of bicyclic heteroaryl groups include, but are
not limited to, indolyl, benzothiazolyl, benzoxazolyl,
benzothienyl, quinolinyl, tetrahydroisoquinolinyl, isoquinolinyl,
benzimidazolyl, benzopyranyl, indolizinyl, benzofuranyl,
isobenzofuranyl, chromonyl, coumarinyl, cinnolinyl, quinoxalinyl,
indazolyl, purinyl, pyrrolopyridinyl, furopyridinyl,
thienopyridinyl, dihydroisoindolyl, and tetrahydroquinolinyl.
Examples of tricyclic heteroaryl groups include, but are not
limited to, carbazolyl, benzindolyl, phenanthrollinyl, acridinyl,
phenanthridinyl, and xanthenyl. In certain embodiments, a
heteroaryl group may optionally be substituted with one or more
substituents Q as described herein.
The term "optionally substituted" is intended to mean that a group,
including alkyl, alkoxy, haloalkyl, cycloalkyl, heterocyclyl, aryl
and heteroaryl, may be substituted with one or more substituents Q
(in one embodiment, one, two, three or four substituents Q), where
each Q is independently selected from the group consisting of
cyano, halo, oxo, nitro, C.sub.1-6 alkyl, C.sub.1-6 alkoxy,
halo-C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, heterocyclyl,
C.sub.6-14 aryl, heteroaryl, --C(O)R.sup.e, --C(O)OR.sup.e,
--C(O)NR.sup.fR.sup.g, --C(NR.sup.e)NR.sup.fR.sup.g, --OR.sup.e,
--OC(O)R.sup.e, --OC(O)OR.sup.e, --OC(O)NR.sup.fR.sup.g,
--OC(.dbd.NR.sup.e)NR.sup.fR.sup.g, --OS(O)R.sup.e,
--OS(O).sub.2R.sup.e, --OS(O)NR.sup.fR.sup.g,
--OS(O).sub.2NR.sup.fR.sup.g, --NR.sup.fR.sup.g,
--NR.sup.eC(O)R.sup.f, --NR.sup.eC(O)OR.sup.f,
--NR.sup.eC(O)NR.sup.fR.sup.g,
--NR.sup.eC(.dbd.NR.sup.h)NR.sup.fR.sup.g, --NR.sup.eS(O)R.sup.f,
--NR.sup.eS(O).sub.2R.sup.f, --NR.sup.eS(O)NR.sup.fR.sup.g,
--NR.sup.eS(O).sub.2NR.sup.fR.sup.g, --SR.sup.e, --S(O)R.sup.e,
--S(O).sub.2R.sup.e, and --S(O).sub.2NR.sup.fR.sup.g, wherein each
R.sup.e, R.sup.f, R.sup.g, and R.sup.h is independently hydrogen,
C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, heterocyclyl, C.sub.6-14
aryl, or heteroaryl; or R.sup.f and R.sup.g, together with the N
atom to which they are attached, form heterocyclyl.
B. CNP Variants
The use of CNP22 as a therapeutic is limited by its short half-life
in plasma (J. Clin. Endocrinol. Metab., 78: 1428-35 (1994)). In
human plasma, the concentration of CNP22 typically is less than
five picomolar. CNP22 is degraded and cleared from circulation by
NEP and NPR-C in humans (Growth Hormone & IGF Res., 16:
S6-S14). In all human and animal studies using systemically
administered CNP22, continuous infusion has been used to increase
the CNP22 concentration in the subjects. A CNP peptide having a
longer half-life and at least a similar level of functionality
would be beneficial to a CNP-based therapeutic strategy. CNP
variants are also disclosed in related International Application
No. PCT/US08/84270, specifically incorporated herein by
reference.
The present disclosure provides CNP variants which have reduced
affinity to NEP and/or NPR-C, and reduced susceptibility to
cleavage by NEP and/or clearance by NPR-C, but which have
substantially similar or better functionality than wild-type CNP22.
Reduced susceptibility of CNP variants to cleavage by NEP and/or
clearance by NPR-C would increase the plasma or serum half-life of
the variants, thereby increasing the opportunity for the variants
to distribute to the target tissues and sites and effectuate the
desired pharmacological effects. In certain embodiments, the CNP
variants described herein have reduced susceptibility to cleavage
by NEP and/or clearance by NPR-C in vitro or in vivo by at least
about 1.5-fold, 2-fold, 2.5-fold, 3-fold, 3.5-fold, 4-fold,
4.5-fold, or 5-fold compared to wtCNP22, and have increased plasma
or serum half-life in vivo by at least about 1.5-fold, 2-fold,
2.5-fold, 3-fold, 3.5-fold, 4-fold, 4.5-fold, or 5-fold compared to
wtCNP22, while retaining at least about 50%, 60%, 70%, 80%, 90% or
100% of the functionality of wtCNP22, or having at least about
1.5-fold, 2-fold, 2.5-fold, 3-fold, 3.5-fold, 4-fold, 4.5-fold, or
5-fold greater functionality than wtCNP22. CNP functionality can be
evaluated in terms of, e.g., the level of one or more biomarkers
(e.g., cGMP) associated with cartilage or bone formation or growth
in an in vitro or in vivo study, the length of particular bones in
an ex vivo or in vivo study, etc.
Natural substrates of NEP are small and natriuretic peptides (about
2.2 to about 3.2 kDa) are the largest of the natural substrates.
According to X-ray crystallographic analyses, the NEP active-site
is buried deep inside a central cavity, effectively restricting the
size of substrate molecules to no more than about 3 kDa (Oefner et
al., J. Mol. Biol., 296: 341-349 (2000)). Based on NPR-B signaling
studies, variants of CNP-22, such as CNP-17 (retaining only the
cyclic domain, Cys6-Cys22, of CNP22) and CNP-53 (CNP-22 with a
31-amino acid extension at the N-terminus), can still bind and
activate NPR-B similarly to the 2.2 kDa wtCNP-22. Accordingly, the
disclosure encompasses CNP variants conjugated to a natural (e.g.,
peptide) and/or synthetic (e.g., PEG) polymer at the N-terminus
and/or C-terminus of CNP22 or variants thereof, which exhibit
increased NEP resistance but retain the ability to bind and
activate the NPR-B signaling receptor.
In one embodiment, the disclosure encompasses CNP variants
represented by the general formula:
(x)-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub-
.12-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.-
sub.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID NO:139), or
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5
-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub.12-
-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub-
.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID NO:140),
wherein:
(x) and (z) each independently are a natural polymer (e.g., a
peptide sequence containing at least one amino acid) and/or a
synthetic polymer (e.g., PEG) as described herein, such that the
total mass of the CNP variant is characterized by the ranges
described generally herein, e.g., in the range from about 2.6 kDa
or 2.8 kDa to about 6 or 7 kDa. In one embodiment, the residues
from Cys6 to Cys22 form a cyclic portion. In an embodiment, (x)
and/or (z) comprise an amino acid extension derived from NPPC or a
non-CNP polypeptide (e.g., ANP, BNP, IgG, etc.), wherein the
extension contains 1 to 40, 1 to 35, 1 to 31, 5 to 35, 5 to 31 or 5
to 15 amino acids. In another embodiment, the CNP variants comprise
one or more modifications and/or substitutions with another natural
amino acid, an unnatural amino acid, a peptidomimetic and/or a
peptide bond isostere at one or more of the following positions of
CNP22: Gly1, Lys4, Gly5, Cys6, Phe7, Gly8, Leu9, Lys10, Leu11,
Ile14, Gly15, Ser16, Met17, Gly19, Leu20 and Gly21.
In another embodiment, CNP variants having a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 kDa or 2.8 kDa to about 6 or 7 kDa, designed for
increased resistance to NEP degradation, are represented by the
general formula:
(x)-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-(h).sub.10-Leu.sub.11-Asp.sub-
.12-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.-
sub.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID NO: 141),
or
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(b).sub.4-Gly.sub.5
-Cys.sub.6-Phe.sub.7-Gly.sub.8-Leu.sub.9-(h).sub.10-Leu.sub.11-Asp.sub.12-
-Arg.sub.13-Ile.sub.14-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub-
.19-Leu.sub.20-Gly.sub.21-Cys.sub.22-(z) (SEQ ID NO: 6),
wherein:
(x) is a synthetic or natural polymeric group, or a combination
thereof, wherein a non-limiting example of a synthetic polymeric
group is PEG (or PEO), and a non-limiting example of a natural
polymeric group is an amino acid sequence containing from 1 to 35
amino acids and derived from NPPC or variants thereof with
substitutions and/or deletions, ANP, BNP, or other non-CNP
(poly)peptides such as, e.g., serum albumin, IgG, histidine-rich
glycoproteins, fibronectin, fibrinogen, zinc finger-containing
polypeptides, osteocrin or fibroblast growth factor 2 (FGF2);
(z) may be absent or may be a synthetic or natural polymeric group,
or a combination thereof, wherein a non-limiting example of a
synthetic polymeric group is PEG, and a non-limiting example of a
natural polymeric group is an amino acid sequence derived from a
natriuretic polypeptide (e.g., NPPC, CNP, ANP or BNP) or
non-natriuretic polypeptide (e.g., serum albumin or IgG); and
(b) and (h) independently may be the wild type Lys at that position
or may be replaced with a conservative amino acid substitution or
any natural or unnatural amino acid or peptidomimetic that does not
have a reactive primary amine on a side chain, including but not
limited to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln,
Glu or Ser. In one embodiment, (b) is Arg. In another embodiment,
for improved NEP resistance, (b) is not Gly. In yet another
embodiment, (h) is not Arg.
Non-limiting examples of amino acid sequences derived from NPPC or
variants thereof include:
TABLE-US-00010 Arg, Glu-Arg, (SEQ ID NO: 7) Gly-Ala-Asn-Lys-Lys,
(SEQ ID NO: 8) Gly-Ala-Asn-Arg-Arg, (SEQ ID NO: 9)
Gly-Ala-Asn-Pro-Arg, (SEQ ID NO: 10) Gly-Ala-Asn-Gln-Gln, (SEQ ID
NO: 11) Gly-Ala-Asn-Ser-Ser, (SEQ ID NO: 12) Gly-Ala-Asn-Arg-Gln,
(SEQ ID NO: 13) Gly-Ala-Asn-Arg-Met, (SEQ ID NO: 14)
Gly-Ala-Asn-Arg-Thr, (SEQ ID NO: 15) Gly-Ala-Asn-Arg-Ser, (SEQ ID
NO: 16) Ala-Ala-Trp-Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn- Ala, (SEQ
ID NO: 17) Ala-Ala-Trp-Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-
Ala-Arg, (SEQ ID NO: 18)
Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp- Ala-Arg, (SEQ ID
NO: 19) Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala-
Asn-Lys-Lys, (SEQ ID NO: 20)
Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-Tyr-Lys-Gly-Ala- Asn-Arg-Arg, (SEQ
ID NO: 21) Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp-
Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-
Tyr-Lys-Gly-Ala-Asn-Lys-Lys, and (SEQ ID NO: 22)
Asp-Leu-Arg-Val-Asp-Thr-Lys-Ser-Arg-Ala-Ala-Trp-
Ala-Arg-Leu-Leu-Gln-Glu-His-Pro-Asn-Ala-Arg-Lys-
Tyr-Lys-Gly-Ala-Asn-Arg-Arg.
Non-limiting examples of amino acid sequences derived from non-CNP
polypeptides such as, e.g., ANP, BNP, serum albumin and IgG
include:
TABLE-US-00011 (SEQ ID NO: 23) Ser-Leu-Arg-Arg-Ser-Ser; (SEQ ID NO:
24) Asn-Ser-Phe-Arg-Tyr; (SEQ ID NO: 25)
Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly; (SEQ ID NO: 26)
Met-Val-Gln-Gly-Ser-Gly; (SEQ ID NO: 27) Lys-Val-Leu-Arg-Arg-Tyr;
(SEQ ID NO: 28) Lys-Val-Leu-Arg-Arg-His; (SEQ ID NO: 29)
Gly-Gln-His-Lys-Asp-Asp-Asn-Pro-Asn-Leu-Pro-Arg; (SEQ ID NO: 30)
Gly-Val-Pro-Gln-Val-Ser-Thr-Ser-Thr; (SEQ ID NO: 31)
Gly-Glu-Arg-Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg- Leu-Ser-Gln; (SEQ
ID NO: 32) Gly-Gln-Pro-Arg-Glu-Pro-Gln-Val-Tyr-Thr-Leu-Pro-
Pro-Ser.
In an embodiment, the N-terminal (x) group and/or the C-terminal
(z) group of any of the CNP variants having an (x) and/or (z)
group, as described herein, independently comprise an amino acid
sequence that contains a small number of, if any, acidic natural or
unnatural amino acids (e.g., Asp or Glu). In another embodiment,
(x) and/or (z) are enriched in basic natural or unnatural amino
acids (e.g., Lys, Arg or His) to maintain an alkaline pI similar to
the pI of CNP22 (pI 8.9). In one embodiment, the pI of the CNP
variants is in the range from about 8 to about 10.5, designed so
that the CNP variants can diffuse more readily through the
extracellular matrix surrounding chondrocytes of bone growth
plates. In narrower embodiments, the pI of the CNP variants is from
about 8.5 to about 10.5, or from about 8.5 to about 10, or from
about 9 to about 10.
In yet another embodiment, (x) and/or (z) are enriched in polar
natural or unnatural amino acids, designed for increased aqueous
solubility. In still another embodiment, (x) and/or (z) contain a
small number of, if any, hydrophobic natural or unnatural amino
acids (e.g., Ala, Val, Leu, Ile or Met).
In a further embodiment, the N-terminus of the CNP variants
terminates in at least one glycine residue, designed for increased
serum half-life. In a related embodiment, to prevent pyroglutamine
formation, the N-terminus of CNP variants terminates in a glycine
residue if it would otherwise terminate in glutamine. In one
embodiment, the (x) group contains an amino acid extension whose
N-terminus terminates in at least one glycine residue. In another
embodiment, (x) and/or (z) do not contain two adjacent basic
natural or unnatural amino acids (e.g., Lys-Lys or Arg-Arg),
designed to reduce susceptibility to cleavage by the protease
furin. In an embodiment, (x) does not contain two adjacent basic
amino acids immediately preceding the position corresponding to
Gly1 of CNP22.
In still another embodiment, the (x) group and/or the (z) group of
the CNP variants comprise an amino acid sequence derived from NPPC
(e.g., derived from CNP53). In an embodiment, (x) comprises an
amino acid sequence derived from the N-terminal tail of ANP or BNP.
In another embodiment, (z) comprises an amino acid sequence derived
from the C-terminal tail of ANP or BNP. In a further embodiment,
(x) and/or (z) comprise an amino acid sequence derived from a
non-natriuretic polypeptide such as, e.g., IgG, human serum albumin
(HSA), histidine-rich glycoproteins, fibronectin, fibrinogen, zinc
finger-containing polypeptides, FGF-2, and bone-targeting proteins
(e.g., osteocrin, osteopontin, osteocalcin, and sialoprotein).
In any embodiment described herein in which CNP22 or a variant
thereof can have an N-terminal (x) group and/or a C-terminal (z)
group, (x) and/or (z) independently can contain an amino acid
sequence derived from the functional domain of a bone morphogenetic
protein (BMP). An N-terminal and/or C-terminal amino acid extension
derived from the functional domain of a BMP can increase the NEP
resistance, and hence the serum half-life of the CNP variant, by
increasing the total mass of the CNP variant to characterized by
the ranges described generally herein, e.g., a range from about 2.6
kDa or 2.8 kDa to about 6 or 7 kDa. In addition, since certain BMPs
are growth factors and cytokines that induce the formation of bone
and cartilage, a fragment derived from the functional domain of a
BMP can promote chondrocyte, cartilage or bone growth by a
mechanism distinct from activation of the guanylyl cyclase function
of NPR-B by the cyclic domain of CNP22 or a variant thereof.
Non-limiting examples of BMPs that promote bone formation and
development, cartilage formation and development, and/or osteoblast
differentiation include BMP1, BMP2, BMP3, BMP5, BMP7 and BMP8a. In
an embodiment, the N-terminus and/or C-terminus of CNP22 or a
variant thereof independently are conjugated to an amino acid
sequence derived from the last 140 amino acids in the C-terminal
portion of BMP1, BMP2, BMP3, BMP5, BMP7 or BMP8a.
In one embodiment, the CNP variants contain an amino acid extension
at the N-terminus and/or C-terminus of CNP22 or CNP17, including
but not limited to:
TABLE-US-00012 (SEQ ID NO: 4)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC (CNP-53);
(SEQ ID NO: 60) QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-37,
Analog BL); (SEQ ID NO: 61) AAWARLLQEHPNAGLSKGCFGLKLDRIGSMSGLGC
(Analog CA); (SEQ ID NO: 62) AAWARLLQEHPNARGLSKGCFGLKLDRIGSMSGLGC
(Analog CB); (SEQ ID NO: 63) DLRVDTKSRAAWARGLSKGCFGLKLDRIGSMSGLGC
(Analog CC); (SEQ ID NO: 40) RGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO:
38) ERGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 64)
GANQQGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 65)
GANRRGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 66)
GANPRGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 67)
GANSSGLSKGCFGLKLDRIGSMSGLGC; (SEQ ID NO: 144)
GHKSEVAHRFKGANKKGLSKGCFGLKLDRIGSMSGLGC; and (SEQ ID NO: 68)
SPKMVQGSG-CNP17-KVLARH (Analog CD) (CNP17 having N-terminal and
C-terminal tails derived from BNP).
In another embodiment, the CNP variants have a K4R substitution at
position 4 of CNP22. Non-limiting examples of CNP(K4R) variants
include:
TABLE-US-00013 (SEQ ID NO: 36) GANRRGLSRGCFGLKLDRIGSMSGLGC (Analog
AY); (SEQ ID NO: 37) GANPRGLSRGCFGLKLDRIGSMSGLGC (Analog CI); (SEQ
ID NO: 41) RGLSRGCFGLKLDRIGSMSGLGC (Analog AZ); (SEQ ID NO: 39)
ERGLSRGCFGLKLDRIGSMSGLGC (Analog BA); (SEQ ID NO: 69)
GANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CH); and (SEQ ID NO: 70)
GANSSGLSRGCFGLKLDRIGSMSGLGC (Analog CG).
In further embodiments, the CNP variants are chimeras comprising
CNP22, or a variant thereof having amino acid addition(s),
deletion(s) and/or substitution(s), and a peptide fragment derived
from a polypeptide or protein other than CNP, or the whole non-CNP
polypeptide or protein, to the N-terminus of the CNP peptide,
wherein CNP22 or the variant thereof may optionally have an
N-terminal amino acid extension of one or more amino acid residues.
In certain embodiments, the CNP chimeras comprise CNP22 or a
variant thereof that has an N-terminal amino acid extension of one
or more amino acid residues. In certain embodiments, the CNP
chimeras contain lysine-lysine (KK) residues or GANKK residues
immediately preceding the first position of CNP22 (Gly in the case
of CNP22) or a variant thereof. In other embodiments, the CNP
chimeras contain one or two residues different from lysine-lysine
immediately preceding the first position of CNP22 or a variant
thereof. Non-limiting examples of residues that can immediately
precede the first position of CNP22 or a variant thereof include
KP, PK, PR, PQ, QK, QQ, RR, SS, GANKP(SEQ ID NO: 200), GANPK (SEQ
ID NO: 201), GANPR (SEQ ID NO: 9), GANPQ (SEQ ID NO: 202), GANQK
(SEQ ID NO: 203), GANQQ (SEQ ID NO: 10), GANRR (SEQ ID NO: 8), and
GANSS (SEQ ID NO: 11).
In another embodiment, the CNP variants are chimera comprising
CNP22 and an N-terminal peptide fragment, including but not limited
to:
TABLE-US-00014 (SEQ ID NO: 76)
GHHSHEQHPHGANQQGLSKGCFGLKLDRIGSMSGLGC (Analog CQ) (histidine-rich
glycoprotein (HRGP) fragment-CNP22 chimera); (SEQ ID NO: 77)
GAHHPHEHDTHGANQQGLSKGCFGLKLDRIGSMSGLGC (Analog CR) (HRGP
fragment-CNP22 chimera); (SEQ ID NO: 78)
GHHSHEQHPHGANPRGLSKGCFGLKLDRIGSMSGLGC (Analog CX) (HRGP
fragment-CNP22 chimera); (SEQ ID NO: 79)
GQPREPQVYTLPPSGLSKGCFGLKLDRIGSMSGLGC (Analog CF) (IgG.sub.1(F)
fragment-CNP22 chimera); (SEQ ID NO: 80)
GQHKDDNPNLPRGANPRGLSKGCFGLKLDRIGSMSGLGC (Analog CY) (human serum
albumin (HSA) fragment- CNP22 chimera); (SEQ ID NO: 81)
GERAFKAWAVARLSQGLSKGCFGLKLDRIGSMSGLGC (Analog CE) (HSA
fragment-CNP22 chimera); (SEQ ID NO: 82)
FGIPMDRIGRNPRGLSKGCFGLKLDRIGSMSGLGC (Analog CZ) (osteocrin "NPR C
inhibitor" fragment-CNP22 chimera); and (SEQ ID NO: 83)
GKRTGQYKLGSKTGPGPKGLSKGCFGLKLDRIGSMSGLGC (Analog DA) (FGF2
"heparin-binding domain" fragment-CNP22 chimera).
In yet another embodiment, the CNP variants are chimera comprising
an N-terminal peptide fragment and CNP22 in which arginine is
substituted for Lys4 of CNP22 ("CNP22(K4R)"), including but not
limited to:
TABLE-US-00015 (SEQ ID NO: 84)
GQPREPQVYTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CK) (IgG.sub.1(F)
fragment-CNP22(K4R) chimera); (SEQ ID NO: 85)
GVPQVSTSTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CL) (HSA
fragment-CNP22(K4R) chimera); (SEQ ID NO: 86)
GQPSSSSQSTGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CM) (fibronectin
fragment-CNP22(K4R) chimera); (SEQ ID NO: 87)
GQTHSSGTQSGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CN) (fibrinogen
fragment-CNP22(K4R) chimera); (SEQ ID NO: 88)
GSTGQWHSESGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CO) (fibrinogen
fragment-CNP22(K4R) chimera); and (SEQ ID NO: 89)
GSSSSSSSSSGANQQGLSRGCFGLKLDRIGSMSGLGC (Analog CP) (zinc finger
fragment-CNP22(K4R) chimera).
Chimera comprising IgG and CNP22 or a variant thereof are designed
for, inter alia, increased resistance to NEP degradation and
reduced binding to serum albumin. CNP chimera comprising a surface
fragment of HSA are designed for, inter alia, reduced
immunogenicity and reduced binding to serum albumin. HRGP-CNP22 and
HRGP-CNP22(K4R) chimera containing a cationic, histidine-rich,
non-lysine, non-arginine sequence at the N-terminus are designed
for, inter alia, increased stability to proteases. Chimera
containing an osteocrin fragment are designed to release, upon
protease (e.g., furin) cleavage, the osteocrin fragment at bone
growth plates, where the fragment would inhibit the clearance
receptor NPR-C. With respect to chimera comprising an FGF2
heparin-binding fragment, heparin binding to the fragment is
designed to protect the chimera from degradation, thereby providing
a longer serum half-life. Chimera containing a fibronectin,
fibrinogen, or zinc-finger fragment are designed for reduced
binding to serum albumin, among other features.
Not intending to be bound by theory, a CNP variant of molecular
weight from about 2.6 or 2.8 kDa to about 6 or 7 kDa which has
increased resistance to NEP degradation and has similar or improved
functionality (e.g., binding to NPR-B and stimulation of cGMP
signaling) as compared to wtCNP22, may be more effective if it does
not bind tightly to plasma proteins such as serum albumin. A CNP
variant that does not bind tightly to plasma proteins (e.g., serum
albumin) may be more effective in diffusing through cartilage,
getting to chondrocytes of bone growth plates, and binding to and
activating NPR-B for cGMP signaling. In one embodiment, CNP
variants designed for reduced binding to plasma proteins (e.g.,
serum albumin) are chimeras comprising CNP22 or a variant thereof
and a peptide fragment from IgG. In another embodiment, CNP
variants designed for reduced binding to plasma proteins are
chimeras comprising CNP22 or CNP22(K4R) and a fragment from a
polypeptide (e.g., IgG, HSA, fibronectin, fibrinogen, a zinc
finger-containing polypeptide, etc.). In yet another embodiment,
CNP variants designed for reduced binding to plasma proteins
comprise CNP22 or a variant thereof conjugated to a hydrophilic or
water-soluble polymer. In one embodiment, the hydrophilic or
water-soluble polymer is PEG (or PEO). In another embodiment, the
hydrophilic or water-soluble polymer (e.g., PEG) is functionalized
with one or more functional groups that impart a negative charge to
the polymer under physiological conditions, such as, e.g, carboxyl,
sulfate or phosphate groups, or a combination thereof.
In a further embodiment, CNP variants of the disclosure include
truncated CNP peptides ranging from human CNP-17 (hCNP-17) to human
CNP-53 (hCNP-53), and having wild-type amino acid sequences derived
from hCNP-53. Such truncated CNP peptides include:
TABLE-US-00016 (SEQ ID NO: 4)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSG LGC (CNP-53);
(SEQ ID NO: 146) LAVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGL
GC (CNP-52); (SEQ ID NO: 147)
RVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGL GC (CNP-51); (SEQ
ID NO: 148) VDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC
(CNP-50); (SEQ ID NO: 149)
DTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-49); (SEQ ID
NO: 150) TKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-48);
(SEQ ID NO: 151) KSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC
(CNP-47); (SEQ ID NO: 152)
SRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-46); (SEQ ID
NO: 153) RAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-45);
(SEQ ID NO: 154) AAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC
(CNP-44); (SEQ ID NO: 155)
AWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-43); (SEQ ID NO:
156) WARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-42); (SEQ ID
NO: 157) ARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-41); (SEQ
ID NO: 158) RLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-40); (SEQ
ID NO: 159) LLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-39); (SEQ
ID NO: 160) LQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-38); (SEQ
ID NO: 60) QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-37); (SEQ ID
NO: 161) EHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-36); (SEQ ID NO:
162) HPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-35); (SEQ ID NO: 163)
PNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-34); (SEQ ID NO: 164)
NARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-33); (SEQ ID NO: 165)
ARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-32); (SEQ ID NO: 166)
RKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-31); (SEQ ID NO: 167)
KYKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-30); (SEQ ID NO: 168)
YKGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-29); (SEQ ID NO: 169)
KGANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-28); (SEQ ID NO: 170)
GANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-27); (SEQ ID NO: 171)
ANKKGLSKGCFGLKLDRIGSMSGLGC (CNP-26); (SEQ ID NO: 172)
NKKGLSKGCFGLKLDRIGSMSGLGC (CNP-25); (SEQ ID NO: 173)
KKGLSKGCFGLKLDRIGSMSGLGC (CNP-24); (SEQ ID NO: 174)
KGLSKGCFGLKLDRIGSMSGLGC (CNP-23); (SEQ ID NO: 1)
GLSKGCFGLKLDRIGSMSGLGC (CNP-22); (SEQ ID NO: 175)
LSKGCFGLKLDRIGSMSGLGC (CNP-21); (SEQ ID NO: 176)
SKGCFGLKLDRIGSMSGLGC (CNP-20); (SEQ ID NO: 177) KGCFGLKLDRIGSMSGLGC
(CNP-19); (SEQ ID NO: 178) GCFGLKLDRIGSMSGLGC (CNP-18); and (SEQ ID
NO: 2) CFGLKLDRIGSMSGLGC (CNP-17).
In certain embodiments, CNP variants do not include CNP-17, CNP-22
or CNP-53.
In another embodiment, the truncated CNP peptides ranging from
hCNP-17 to hCNP-53 can contain amino acid addition(s), deletion(s)
and/or substitution(s) with natural or unnatural amino acid(s) or
peptidomimetic(s) (e.g., peptide bond isostere(s)), as described
herein, at any one or more of the amino acid positions of the
particular truncated CNP peptides. In yet another embodiment, the
truncated CNP peptides having wild-type sequences or amino acid
addition(s), deletion(s) and/or substitution(s), can be conjugated
at the N-terminus, C-terminus and/or internal site(s) to any of the
moieties described herein, including but not limited to bone- or
cartilage-targeting moieties (e.g., bisphosphonates, bone- or
cartilage-targeting peptide sequences (e.g., polyAsp, poly-Glu),
peptide sequences derived from bone-targeting domains of bone
proteins (e.g., osteopontin, osteocalcin, sialoprotein)), peptide
sequences derived from the functional domains of bone morphogenetic
proteins (e.g., BMP2, BMP3, BMP5, BMP7, BMP8a), peptide sequences
derived from natriuretic polypeptides (e.g., NPPC, ANP, BNP),
peptide sequences derived from polypeptides of non-natriuretic
origin (e.g., serum albumin, IgG, histidine-rich glycoproteins,
fibronectin, fibrinogen, zinc finger-containing polypeptides,
FGF-2, osteocrin), moieties that reduce renal clearance (e.g.,
negatively charged PEG moieties), hydrophilic polymers (e.g., PEG),
carbohydrates (e.g., carbohydrates recognized by receptors on the
surface of cells at bone growth plates), hydrophobic acids (e.g.,
C.sub.5-C.sub.12 carboxylic acids, natural fatty acids),
phospholipids, and combinations thereof. In an embodiment, the
truncated CNP peptides having wild-type sequences or amino acid
addition(s), deletion(s) and/or substitution(s), and optionally
conjugated to one or more moieties at the N-terminus, C-terminus
and/or internal site(s), have a total mass characterized by the
ranges described generally herein, e.g., from about 2.6 kDa or 2.8
kDa to about 6 or 7 kDa.
In a further embodiment, the CNP variants are derivatives of CNP37,
which is QEHPNARKYKGANKK-CNP22 (SEQ ID NO: 60). The CNP37 variants
can contain amino acid addition(s), deletion(s), and/or
substitution(s) with natural or unnatural amino acid(s) or
peptidomimetic(s) (e.g., peptide bond isostere(s)) at any one or
more of the 37 positions of CNP37. Non-limiting examples of
substitutions that can be made in CNP37, based on the numbering of
CNP22, include K4R, G5S, G5R, G8S, K10R, G15S, S16Q, M17N, G19R,
and combinations thereof. In an embodiment, the CNP37 derivatives
contain a substitution of Met17 to a natural (e.g., asparagine) or
unnatural amino acid or peptidomimetic, designed in part to avoid
oxidation of the sulfur atom of methionine. In another embodiment,
the CNP37 variants contain substitution(s) of Lys8, Lys10, Lys14
and/or Lys15 (based on numbering from the N-terminus of CNP37) to
non-basic natural or unnatural amino acid(s) or peptiomimetic(s),
designed in part to reduce albumin binding.
In addition or alternatively to amino acid addition(s), deletion(s)
and/or substitution(s), the CNP37 derivatives can be conjugated at
the N-terminus, C-terminus, and/or an internal site to any of the
moieties described herein, including but not limited to bone- or
cartilage-targeting moieties (e.g., bone-targeting peptide
domains), moieties that reduce renal clearance (e.g., negatively
charged PEG moieties), hydrophilic polymers (e.g., PEG), amino acid
sequences comprising one or more amino acids (e.g., osteocrin
"NPR-C inhibitor" fragment), carbohydrates (e.g., carbohydrates
recognized by receptors on the surface of cells at bone growth
plates), hydrophobic acids (e.g., C.sub.5-C.sub.12 carboxylic acids
and natural fatty acids), and combinations thereof.
In one embodiment, the CNP variants are modified CNP37 peptides
having mutation(s)/substitution(s) at the furin cleavage site
(underlined), designed to improve in vivo resistance to the furin
protease, and/or containing glycine (underlined) at the N-terminus,
designed to improve plasma stability and prevent pyroglutamine
formation. Such CNP37 variants include but are not limited to:
TABLE-US-00017 (SEQ ID NO: 71)
GQEHPNARKYKGANPKGLSKGCFGLKLDRIGSMSGLGC (An. CS); (SEQ ID NO: 72)
GQEHPNARKYKGANQKGLSKGCFGLKLDRIGSMSGLGC (An. CT); (SEQ ID NO: 73)
GQEHPNARKYKGANQQGLSKGCFGLKLDRIGSMSGLGC (An. CU); (SEQ ID NO: 74)
GQEHPNARKYKGANKPGLSKGCFGLKLDRIGSMSGLGC (An. CW); (SEQ ID NO: 75)
GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Gly-CNP37, An. DB); and
(SEQ ID NO: 145) PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC
(Pro-Gly-CNP37)
In a further embodiment, the CNP variants of the disclosure include
CNP peptides and variants thereof that can be produced by the
fusion protein process described herein. Non-limiting examples of
CNP variants that can be produced by the fusion protein process
described herein, using chemical or proteolytic cleavage or protein
self-cleavage, include:
TABLE-US-00018 (SEQ ID NO: 179)
GDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Gly-wtCNP53); (SEQ ID NO: 75)
GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Gly-wtCNP37); (SEQ ID NO:
60) QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (wtCNP37); (SEQ ID NO:
144) GHKSEVAHRFK-GANKKGLSKGCFGLKLDRIGSMSGLGC (HSA
fragment-wtCNP27); (SEQ ID NO: 36) GANRRGLSRGCFGLKLDRIGSMSGLGC
[CNP27(K4,5,9R)]; (SEQ ID NO: 180)
DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSG LGC
[CNP53(M48N)]; (SEQ ID NO: 181)
GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSGLGC [Gly-CNP37(M32N)]; (SEQ ID
NO: 182) QEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSGLGC [CNP37(M32N)]; (SEQ
ID NO: 183) GHKSEVAHRFK-GANKKGLSKGCFGLKLDRIGSNSGLGC
[HSA-CNP27(M22N)]; (SEQ ID NO: 184) GANRRGLSRGCFGLKLDRIGSNSGLGC
[CNP27(K4,5,9R, M22N)]; (SEQ ID NO: 185)
PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Pro-wtCNP53); (SEQ ID NO: 145)
PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Pro-Gly-wtCNP37); (SEQ ID
NO: 186) PQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Pro-wtCNP37); (SEQ
ID NO: 187) PNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (wtCNP34); (SEQ ID
NO: 188) P-GHKSEVAHRFK-GANKKGLSKGCFGLKLDRIGSMSGLGC
(Pro-HSA-wtCNP27); (SEQ ID NO: 189) PGANRRGLSRGCFGLKLDRIGSMSGLGC
[Pro-CNP27(K4,5,9R)]; (SEQ ID NO: 190)
MDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMS GLGC
(Met-wtCNP53); (SEQ ID NO: 191)
MGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Met-Gly-wtCNP37); (SEQ ID
NO: 192) MQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (Met-wtCNP37); (SEQ
ID NO: 193) M-GHKSEVAHRFK-GANKKGLSKGCFGLKLDRIGSMSGLGC
(Met-HSA-wtCNP27); (SEQ ID NO: 194) MGANRRGLSRGCFGLKLDRIGSMSGLGC
[Met-CNP27(K4,5,9R)].
Other CNP variants, including truncated CNP peptides ranging from
hCNP-17 to hCNP-53 and having wild-type sequences or amino acid
addition(s), deletion(s) and/or substitution(s), can also be
produced by the fusion protein process described herein, so long as
the intended site of chemical or proteolytic cleavage of the fusion
protein is not present within the amino acid sequence of the target
CNP variant itself. As a non-limiting example, the fusion protein
process described herein can be employed to produce truncated
wtCNP34 using formic acid cleavage.
In additional embodiments, for any of the CNP peptides and CNP
variants described herein that have asparagine (Asn/N) residue(s)
and/or glutamine (Gln/Q) residue(s), whether they have a wild-type
sequence or a non-natural amino acid sequence, any Asn residue(s)
and/or any Gln residue(s) can independently be substituted with any
other natural or unnatural amino acids, including conservative
substitutions such as Asn to Gln. Such substitution(s) are designed
in part to minimize or avoid any potential deamidation of
asparagine and/or glutamine. Non-limiting examples of CNP peptides
and variants in which any Asn residue(s) and/or any Gln residue(s)
can independently be substituted with any other natural or
unnatural amino acids, including conservative substitutions such as
Asn to Gln, include wtCNP34, wtCNP37, Gly-wtCNP37, Pro-wtCNP37,
Pro-Gly-wtCNP37, GHKSE-VAHRFK-wtCNP27 (SEQ ID NO: 144),
Pro-GHKSE-VAHRFK-wtCNP27 (SEQ ID NO: 188), PEO12-GANRR-CNP22(K4R)
(SEQ ID NO: 36), and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36). In
certain embodiments, an asparagine residue of the CNP peptides and
CNP variants described herein is not substituted with glutamine,
aspartic acid or glutamic acid. In certain embodiments, a glutamine
residue of the CNP peptides and CNP variants described herein is
not substituted with asparagine, aspartic acid or glutamic acid. As
a non-limiting example, asparagine residues 7 and/or 15 of
Pro-Gly-wtCNP37 (PGQEHP NARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC) SEQ ID
NO: 145) can independently be substituted with any other natural or
unnatural amino acids, including glutamine, to avoid any potential
deamidation of the asparagine residue(s) to aspartic acid or
isoaspartic acid. In certain embodiments, asparagine residues 7
and/or 15 of Pro-Gly-wtCNP37 are not substituted with glutamine,
aspartic acid or glutamic acid.
It is understood, however, that the present disclosure encompasses
CNP variants in which any one or more, up to all, residues
susceptible to deamidation or a deamidation-like reaction (e.g.,
isomerization) may be converted to other residue(s) via deamidation
or a deamidation-like reaction to any extent, up to 100% conversion
per converted residue. In certain embodiments, the disclosure
encompasses CNP variants in which: (1) any one or more, up to all,
asparagine (Asn/N) residues may be converted to aspartic acid or
aspartate, and/or to isoaspartic acid or isoaspartate, via
deamidation up to about 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%,
90% or 100% conversion per converted residue; or (2) any one or
more, up to all, glutamine (Gln/Q) residues may be converted to
glutamic acid or glutamate, and/or to isoglutamic acid or
isoglutamate, via deamidation up to about 5%, 10%, 20%, 30%, 40%,
50%, 60%, 70%, 80%, 90% or 100% conversion per converted residue;
or (3) any one or more, up to all, aspartic acid or aspartate
(Asp/D) residues may be converted to isoaspartic acid or
isoaspartate via a deamidation-like reaction (also called
isomerization) up to about 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%,
80%, 90% or 100% conversion per converted residue; or (4) any one
or more, up to all, glutamic acid or glutamate (Glu/E) residues may
be converted to isoglutamic acid or isoglutamate via a
deamidation-like reaction (also called isomerization) up to about
5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100% conversion
per converted residue; or (5) a combination of the above.
As a non-limiting example, the disclosure encompasses CNP variants
in which any one or more, up to all, asparagine, glutamine,
aspartic acid, and/or glutamic acid residues of Pro-Gly-wtCNP37
[PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC] (SEQ ID NO: 145) may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, (2) glutamic acid/glutamate and/or isoglutamic
acid/isoglutamate, (3) isoaspartic acid/isoaspartate, and/or (4)
isoglutamic acid/isoglutamate, respectively, via deamidation or a
deamidation-like reaction up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% conversion per converted residue, as
described above.
As a further example, the disclosure encompasses CNP variants in
which any one or more, up to all, asparagine and/or aspartic acid
residues of Pro-Gly-wtCNP37
[PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC] (SEQ ID NO: 145) may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, and/or (2) isoaspartic acid/isoaspartate,
respectively, via deamidation or a deamidation-like reaction up to
about 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100%
conversion per converted residue.
As another example, the present disclosure encompasses CNP variants
in which any one or more, up to all, asparagine, glutamine,
aspartic acid, and/or glutamic acid residues of Gly-wtCNP37
[GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 75)] may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, (2) glutamic acid/glutamate and/or isoglutamic
acid/isoglutamate, (3) isoaspartic acid/isoaspartate, and/or (4)
isoglutamic acid/isoglutamate, respectively, via deamidation or a
deamidation-like reaction up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% conversion per converted residue.
As yet another example, the disclosure encompasses CNP variants in
which any one or more, up to all, asparagine, glutamine, aspartic
acid, and/or glutamic acid residues of wtCNP37
[QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 60)] may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, (2) glutamic acid/glutamate and/or isoglutamic
acid/isoglutamate, (3) isoaspartic acid/isoaspartate, and/or (4)
isoglutamic acid/isoglutamate, respectively, via deamidation or a
deamidation-like reaction up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% conversion per converted residue.
As a further example, the present disclosure encompasses CNP
variants in which any one or more, up to all, asparagine, aspartic
acid, and/or glutamic acid residues of an HSA-wtCNP27 chimera,
GHKSEVAHRFKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 144), may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, (2) isoaspartic acid/isoaspartate, and/or (3)
isoglutamic acid/isoglutamate, respectively, via deamidation or a
deamidation-like reaction up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% conversion per converted residue.
As a still further example, the disclosure encompasses CNP variants
in which any one or more, up to all, asparagine, aspartic acid,
and/or glutamic acid residues of a Pro-HSA-wtCNP27 chimera,
PGHKSEVAHRFKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 188), may be
converted to (1) aspartic acid/aspartate and/or isoaspartic
acid/isoaspartate, (2) isoaspartic acid/isoaspartate, and/or (3)
isoglutamic acid/isoglutamate, respectively, via deamidation or a
deamidation-like reaction up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% conversion per converted residue.
In addition, the present disclosure encompasses CNP variants in
which any one or more, up to all, methionine (Met/M) residues may
be oxidized to any chemically feasible oxidized form (e.g.,
sulfoxide and/or sulfone) up to about 5%, 10%, 20%, 30%, 40%, 50%,
60%, 70%, 80%, 90% or 100% transformation per oxidized residue.
In another embodiment, the CNP variants comprise CNP22 or variants
thereof conjugated at the N-terminus and/or C-terminus to
moiet(ies) that facilitate translocation of the variants across a
cell membrane or cell barrier. In one embodiment, the CNP variants
are conjugated at the N-terminus and/or C-terminus to peptide
sequence(s) that facilitate transport of the variants across a cell
membrane or cell barrier, including via active peptide
transporters.
In a further embodiment, the N-terminus and/or C-terminus of CNP22
or a variant thereof are conjugated to chemical moieties such as,
e.g., natural and/or synthetic polymers, to increase the total mass
of the modified CNP peptide to the ranges described generally
herein, e.g., a range from about 2.6 or 2.8 kDa to about 6 or 7
kDa. In one embodiment, the chemical moieties are biocompatible
hydrophilic or water-soluble natural (e.g., peptides,
carbohydrates) or synthetic (e.g., PEG (or PEO)) polymers.
In a particular embodiment, the N-terminus and/or C-terminus of
CNP22 or a variant thereof are conjugated to PEG (or PEO) polymers
to result in a total mass characterized by the ranges described
generally herein, e.g., from about 2.6 or 2.8 kDa to about 6 or 7
kDa. Pegylation of CNP22 or a variant thereof is designed, inter
alia, to reduce immunogenicity and improve half-life by reducing
renal clearance and increasing protease resistance. A PEG moiety
can be attached to the N- and/or C-terminus of CNP22 or any variant
described herein, including but not limited to CNP-17 (the
Cys6-Cys22 cyclized portion of CNP22), CNP37, and variants of
CNP17, CNP22 or CNP37 having N- and/or C-terminal amino acid
extension(s), amino acid substitution(s) and/or amino acid
deletion(s). In an embodiment, the Lys4 and/or Lys10 residues of
CNP17, CNP22 or CNP37, or variants thereof, are substituted with a
natural or unnatural amino acid (e.g., Arg, Gly, Ser, Gln, Glu or
Cit) or peptidomimetic that does not contain a reactive primary
amine on a side chain, to preclude any potential PEGylation of
these lysine residues. In one embodiment, the Lys4 and/or Lys10
residues of the CNP peptides are substituted with Arg. In another
embodiment, the Lys10 residue is not substituted with Arg.
In a further embodiment, CNP variants (including CNP22 and variants
thereof) having a PEG (or PEO) moiety and an amino acid extension
at the N-terminus contain arginine at the position immediately
preceding the position corresponding to Gly1 of CNP22. Such
PEGylated CNP variants are designed for increased resistance to NEP
degradation, reduced binding to serum albumin, and enhanced CNP
functional activity (e.g., activation of cGMP signaling).
Non-limiting examples of PEGylated CNP variants include
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36), PEO12-GANRR-CNP22(K4R) (SEQ
ID NO: 36), PEO24-GANRR-CNP22(SEQ ID NO: 36), PEO12-GANRR-CNP22(SEQ
ID NO: 36), PEO24-GANPR-CNP22(K4R) (SEQ ID NO: 37),
PEO12-GANPR-CNP22(K4R) (SEQ ID NO: 37), PEO24-GANPR-CNP22(SEQ ID
NO: 37), PEO12-GANPR-CNP22 (SEQ ID NO: 37), PEO24-GANQQ-CNP22(SEQ
ID NO: 64), PEO12-GANQQ-CNP22(SEQ ID NO: 64), PEO24-ER-CNP22(K4R)
(SEQ ID NO: 39), PEO12-ER-CNP22(K4R) (SEQ ID NO: 39),
PEO24-ER-CNP22(SEQ ID NO: 39), PEO12-ER-CNP22(SEQ ID NO: 39),
PEO24-R-CNP22(K4R) (SEQ ID NO: 41), PEO12-R-CNP22(K4R) (SEQ ID NO:
41), PEO24-R-CNP22(SEQ ID NO: 41), and PEO12-R-CNP22(SEQ ID NO:
41), wherein PEO24 is a monodispersed 1.2 kDa PEG polymer and PEO12
is a monodispersed 0.6 kDa PEG polymer. In one embodiment, the PEG
(or PEO) polymer is conjugated to the N-terminus of the CNP
variants.
The disclosure contemplates use of hydrophilic or water soluble
polymers (e.g., PEG) that can vary in type (e.g., homopolymer or
copolymer; random, alternating or block copolymer; linear or
branched; monodispersed or polydispersed), linkage (e.g.,
hydrolysable or stable linkage such as, e.g., amide, imine, aminal,
alkylene, or ester bond), conjugation site (e.g., at the N-terminus
and/or C-terminus, preferably not at any of the residues in the
cyclized region of CNP (corresponding to residues 6-22 of CNP22)),
and length (e.g., from about 0.2, 0.4 or 0.6 kDa to about 2, 3, 4
or 5 kDa). The hydrophilic or water-soluble polymer can be
conjugated to the CNP peptide by means of N-hydroxy succinimide
(NHS)- or aldehyde-based chemistry or other chemistry, as is known
in the art. Such CNP variants can be generated using, e.g., wtCNP22
(2.2 kDa), CNP17 retaining only the cyclized region (residues 6-22)
of wtCNP22, CNP variants having an amino acid extension at the
N-terminus and/or C-terminus of CNP22 or CNP17, or variants having
amino acid substitutions, additions and/or deletions such as, e.g.,
GANRR-CNP22(K4R) (SEQ ID NO: 36), GANPR-CNP22(K4R) (SEQ ID NO: 37),
R-CNP22(SEQ ID NO: 40), R-CNP22(K4R) (SEQ ID NO: 41), ER-CNP22(SEQ
ID NO: 38) and ER-CNP22(K4R) (SEQ ID NO: 39). In an embodiment, the
PEG-CNP variants having a total mass characterized by the ranges
described generally herein, e.g., from about 2.6 or 2.8 kDa to
about 6 or 7 kDa, contain a monodispersed, linear PEG (or PEO)
moiety conjugated at the N-terminus and/or C-terminus via NHS- or
aldehyde-based chemistry, or a two-arm or three-arm branched PEG
moiety conjugated at the N-terminus and/or C-terminus via NHS-based
chemistry. The disclosure also encompasses negatively charged
PEG-CNP variants designed for reduced renal clearance, including
but not limited to carboxylated, sulfated and phosphorylated
compounds (Caliceti, Adv. Drug Deliv. Rev., 55: 1261-77 (2003);
Perlman, J. Clin. Endo. Metab., 88: 3227-35 (2003); Pitkin,
Antimicrob. Ag. Chemo., 29: 440-444 (1986); Vehaskari, Kidney
Int'l, 22: 127-135 (1982)). In one embodiment, the PEG (or PEO)
moiety contains carboxyl group(s), sulfate group(s), and/or
phosphate group(s).
In another embodiment, the PEG (or PEO) moieties conjugated to the
N-terminus, C-terminus and/or internal site(s) of CNP variants
described herein contain one or more functional groups that are
positively charged under physiological conditions. Such PEG
moieties are designed, inter alia, to improve distribution of such
PEGylated CNP variants to cartilage tissues. In one embodiment,
such PEG moieties contain one or more primary, secondary or
tertiary amino groups, quaternary ammonium groups, and/or other
amine-containing (e.g., urea) groups.
In an embodiment, the disclosure encompasses CNP22 or variants
thereof conjugated via NHS- or aldehyde-based chemistry to PEG (or
PEO) of the formula (CH.sub.2CH.sub.2O).sub.n, wherein n is an
integer from about 6 to about 100, and the PEG polymer is from
about 0.3 kDa to about 5 kDa. In another embodiment, n is an
integer from about 12 to about 50, and the PEG polymer is from
about 0.6 kDa to about 2.5 kDa. In yet another embodiment, n is
from about 12 to about 24, and the PEG polymer is from about 0.6
kDa to about 1.2 kDa. In still another embodiment, the terminal
hydroxyl group of the PEG polymer is capped with a non-reactive
group. In a particular embodiment, the end-capping group is an
alkyl group, e.g., a lower alkyl group such as methyl.
In a further embodiment, the disclosure provides CNP variants
having one or more peptide bonds or peptide bond isosteres that
have reduced susceptibility to cleavage by peptidases including
neutral endopeptidase (NEP). NEP is a membrane-bound zinc-dependent
endopeptidase that cleaves a substrate peptide bond at the amino
end of large hydrophobic residues. Thus, modification of a peptide
bond at a cleavage site for NEP to an unnatural peptide or
non-peptide bond may preclude or decrease the efficiency of NEP
cleavage.
For ANP and CNP, NEP cleavage is reported to occur first at the
Cys6-Phe7 bond within the cyclized region, then elsewhere
throughout the remainder of the structures. For BNP, cleavage is
reported to occur first at the peptide N-terminus, then within the
cyclic structure. Although the primary NEP cleavage site on CNP is
reported to be the Cys6-Phe7 bond, when wtCNP22 was exposed to NEP
digestion for 2.5 minutes in vitro, all possible sites were
unexpectedly hydrolyzed, with the Cys6-Phe7 and Gly8-Leu9 peptide
bonds being slightly most labile, as described in Example 2.
Substrate specificity of NEP is primarily determined by two
substrate-binding subsites, S1' and S2' (Oefner et al., J. Mol.
Biol. 296:341-349 (2000)). The S1' site accepts a large hydrophobic
P1' residue of which the N-terminal peptide bond is subjected to
hydrolysis (e.g., Phe, Leu, Be and Met). The S2' site generally
prefers a smaller residue, termed P2' (e.g., Gly or Ser). In the
case of CNP, Phe7 is reported to be the preferred P1' residue for
the NEP S1' site, while Gly8 is the preferred P2' residue for the
S2' site. Because these two subsites can together accommodate only
a certain total side chain size, any increase in the total size of
the P1'-P2' residues of CNP can potentially disrupt NEP binding.
For example, addition of a chloride atom at the 3-position of the
P1' Phe7 aromatic ring (i.e., 3-Cl-Phe7) can potentially modify
(e.g., destabilize) interactions between CNP and the NEP cleavage
sites, for example at the S1' subsite. Addition of a tertiary butyl
group to the smaller P2' residue Gly8 (i.e., tBu-Gly8) can
potentially disrupt the interaction between CNP and the S2'
subsite.
Accordingly, in one embodiment, CNP variants of the disclosure
include CNP having an increase in the size of the P1'-P2' residues,
such as Phe7-Gly8, to interfere with substrate recognition at the
active site, thereby reducing susceptibility to NEP cleavage.
Natural amino acids, unnatural amino acids and/or peptidomimetic
moieties are substituted for one or more large P1' hydrophobic
residues, including but not limited to Phe7, Leu9, Leu11, Ile14,
Met17 and Leu20, and/or for one or more smaller P2' residues,
including but not limited to Cys6, Gly8, Gly15, Ser16 and
Gly19.
The disclosure encompasses CNP variants comprising at least one
modified amino acid and/or at least one modified peptide bond, at
at least one residue involved in substrate recognition and/or
cleavage by NEP, wherein the modified amino acids and modified
peptide bonds can be natural amino acids, unnatural amino acids,
peptidomimetics and/or peptide bond isosteres. In one embodiment,
the NEP cleavage site on CNP between Cys6 and Phe7 is modified. In
a related embodiment, the peptide bond (--C(.dbd.O)--NH--) between
Cys6 and Phe7 is replaced with one of the following peptide bond
isosteres:
--CH.sub.2--NH--,
--C(.dbd.O)--N(R)--, where the amide group is alkylated with any of
the following R groups: methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl,
--C(.dbd.O)--NH--CH.sub.2--,
--CH.sub.2--S--,
--CH.sub.2--S(O).sub.n--, where n is 1 or 2,
--CH.sub.2--CH.sub.2--,
--CH.dbd.CH--,
--C(.dbd.O)--CH.sub.2--,
--CH(CN)--NH--,
--CH(OH)--CH.sub.2--,
--O--C(.dbd.O)--NH--, or
--NHC(.dbd.O)NH--.
In another embodiment, the CNP variants are represented by the
formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-Lys.sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-Leu.sub.9-Lys.sub.10-Leu.sub.11-Asp.sub.12-Arg.sub.13-Ile.sub.1-
4-Gly.sub.15-Ser.sub.16-Met.sub.17-Ser.sub.18-Gly.sub.19-Leu.sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 90), wherein:
(x) and (z) independently may be absent or may be selected from the
group consisting of synthetic bone-targeting compounds such as,
e.g., bisphosphonates; amino acid sequences useful in bone or
cartilage targeting such as, e.g., polyAsp and polyGlu; amino acid
sequences derived from bone-targeting domains of bone proteins such
as, e.g., osteopontin, osteocalcin, and sialoprotein (Wang et al.,
Adv. Drug Delivery Rev., 57: 1049-76 (2005)); polymeric and
non-polymeric molecules that reduce renal clearance such as, e.g.,
negatively charged PEGs; and natural polymers (e.g., those
containing amino acids, fatty acids and/or carbohydrates) and
synthetic polymers (e.g., PEGs) that increase resistance of the CNP
variant to NEP degradation by increasing the total mass of the CNP
variant to the ranges described generally herein, e.g., from about
2.6 or 2.8 kDa to about 6 or 7 kDa;
(b) and (c) may be the wild-type Cys6 and Phe7, another natural
amino acid or an unnatural amino acid, or may contain a peptide
bond isostere as described herein to increase resistance to NEP
cleavage; and
(d) may be the wild-type Gly8, or may be a larger natural or
unnatural (e.g., t-Bu-Gly) amino acid or peptidomimetic to reduce
binding to NEP.
In one embodiment, such CNP variants contain at least one modified
amino acid at (b), (c) and/or (d).
Other peptide bonds within CNP may be cleaved even if CNP22 or a
variant thereof has an NEP-resistant peptide bond or peptide bond
isostere at Cys6-Phe7, including the Gly8-Leu9, Lys10-Leu11,
Arg13-Ile14, Ser16-Met17, and Gly19-Leu20 bonds. Therefore, the
disclosure encompasses CNP variants having peptide bond isostere(s)
at one or more other NEP cleavages sites in addition to the
Cys6-Phe7 bond, wherein the peptide bond isosteres include those
described herein.
In another embodiment, the disclosure encompasses CNP variants
having a cysteine analog at Cys6 and/or Cys22, including but not
limited to homocysteine, penicillamine, 2-mercaptopropionic acid,
and 3-mercaptopropionic acid. In an embodiment, such CNP variants
have a cyclic domain formed by a disulfide bond between the
wild-type Cys6 or analog and Cys22 or analog.
In yet another embodiment, one or more residues of CNP22 or a
variant thereof, up to all the residues, are substituted with a
D-amino acid. Substitution of an L-amino acid with a D-amino acid
essentially moves the side chain about 120 degrees from its
original position, thereby potentially disrupting the binding of
the CNP peptide to NEP. In a specific embodiment, L-Phe at Phe7 is
substituted with its D-enantiomer, D-Phe.
In still another embodiment, a beta amino acid such as, e.g.,
3-amino-2-phenylpropionic acid (or 2-phenyl-beta-alanine), replaces
the wild-type alpha-amino acid Phe7. Use of a beta-amino acid
effectively increases the peptide backbone length by one methylene
unit. Protease resistance can result from the change in substrate
conformation or the increased distance between amino acid side
chains.
Non-limiting examples of variants of CNP22 having an unnatural
alpha-amino acid, a beta-amino acid or a peptide bond isostere
include:
TABLE-US-00019 (SEQ ID NO: 56) GLSKGC(CH.sub.2NH)FGLKLDRIGSMSGLGC
(Analog A), (SEQ ID NO: 57) GLSKGC-(N-Me--Phe)-GLKLDRIGSMSGLGC
(Analog B), (SEQ ID NO: 136) GLSKGC-(D-Phe)-GLKLDRIGSMSGLGC (Analog
E), (SEQ ID NO: 58) GLSKGCF-(tBu--Gly)-LKLDRIGSMSGLGC (Analog F),
(SEQ ID NO: 137) GLSKGC-(3-Cl-Phe)-GLKLDRIGSMSGLGC (Analog G), and
(SEQ ID NO: 59) GLSKGC-[NHCH.sub.2CH(Ph)CO]-GLKLDRIGSMSGLGC (Analog
H, formed using 3-amino-2-phenylpropionic acid).
In a further embodiment, the CNP variants have a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 or 2.8 kDa to about 6 or 7 kDa, designed for increased
resistance to NEP degradation, and are represented by the formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(a).sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub.12-Arg.sub.13-(h).sub.1-
4-Gly.sub.15-Ser.sub.16-(i).sub.17-Ser.sub.18-Gly.sub.19-(j).sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO: 46), wherein:
(x) and (z) independently may be absent or may be selected from the
group consisting of synthetic bone-targeting compounds such as,
e.g., bisphosphonates; amino acid sequences useful in bone or
cartilage targeting such as, e.g., polyAsp and polyGlu; amino acid
sequences derived from bone-targeting domains of bone proteins such
as, e.g., osteopontin, osteocalcin, and sialoprotein; polymeric and
non-polymeric moieties that reduce renal clearance such as, e.g.,
negatively charged PEGs; polymers containing, e.g., amino acids,
hydrophobic acids, and/or carbohydrates; and synthetic hydrophilic
polymers such as, e.g., PEGs;
(a) may be the wild-type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (a) is Arg;
(b) is selected from the group consisting of Cys and peptide-bond
isosteres between Cys6 and Phe7 such as, e.g.,
Cys-CH.sub.2--NH;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; N-alkylated derivatives of Phe,
wherein the N-alkyl group is methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl; and Phe
analogs, wherein one or more ortho-, meta-, and/or para-positions
of the benzene ring of the Phe analog are substituted with one or
more substituents selected from the group consisting of halogen,
hydroxyl, cyano, straight or branched C.sub.1-6 alkyl, straight or
branched C.sub.1-6 alkoxy, straight or branched halo-C.sub.1-6
alkyl, C.sub.3-10 cycloalkyl, heterocyclyl, C.sub.6-14 aryl and
heteroaryl (examples include, but are not limited to, tyrosine,
3-chlorophenylalanine, 2,3-chloro-phenylalanine,
3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly
(tBu-Gly), Thr, Ser, Val and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr and
peptide-bond isosteres such as, e.g., N-Me-Leu;
(f) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (f) is not Arg;
(g) is selected from the group consisting of Leu and peptide-bond
isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tBu-Gly, and
peptide-bond isosteres such as, e.g., N-Me-Ile;
(i) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(j) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg,
and peptide-bond isosteres such as, e.g., N-Me-Leu.
In another embodiment, the CNP variants have a total mass
characterized by the ranges described generally herein, e.g., from
about 2.6 or 2.8 kDa to about 6 or 7 kDa, designed for increased
resistance to NEP cleavage, and are represented by the formula:
(x)-Gly.sub.1-Leu.sub.2-Ser.sub.3-(a).sub.4-Gly.sub.5-(b).sub.6-(c).sub.7-
-(d).sub.8-(e).sub.9-(f).sub.10-(g).sub.11-Asp.sub.12-Arg.sub.13-(h).sub.1-
4-(i).sub.15-Ser.sub.16-(j).sub.17-Ser.sub.18-Gly.sub.19-(k).sub.20-Gly.su-
b.21-Cys.sub.22-(z) (SEQ ID NO:143), wherein:
(x) and (z) independently may be absent or may be selected from the
group consisting of synthetic bone-targeting compounds such as,
e.g., bisphosphonates; amino acid sequences useful in bone or
cartilage targeting such as, e.g., polyAsp and polyGlu; amino acid
sequences derived from bone-targeting domains of bone proteins and
derivatives thereof, such as, e.g., fusion proteins or peptide
sequences of osteopontin, osteocalcin, sialoprotein, etc.; moieties
that reduce renal clearance, including but not limited to
hydrophilic or water-soluble polymers such as, e.g., charged PEG
molecules; and moieities comprising, e.g., PEGs, amino acids,
carbohydrates, and/or hydrophobic acids;
(a) may be the wild type Lys at that position or may be replaced
with a conservative amino acid substitution or a natural or
unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, including but not limited
to Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glu, wherein in one embodiment (a) is Arg;
(b) is selected from the group consisting of Cys and peptide bond
isosteres between Cys6 and Phe7 such as, e.g.,
Cys-CH.sub.2--NH;
(c) is selected from the group consisting of L-Phe; D-Phe;
3-amino-2-phenylpropionic acid; N-alkylated derivatives of Phe,
wherein the N-alkyl group is methyl, ethyl, n-propyl, isopropyl,
cyclopropyl, n-butyl, isobutyl, sec-butyl or tert-butyl; and Phe
analogs, wherein one or more ortho-, meta-, and/or para-positions
of the benzene ring of the Phe analog are substituted with one or
more substituents selected from the group consisting of halogen,
hydroxyl, cyano, straight or branched C.sub.1-6 alkyl, straight or
branched C.sub.1-6 alkoxy, straight or branched halo-C.sub.1-6
alkyl, C.sub.3-10 cycloalkyl, C.sub.6-14 aryl, heterocyclyl and
heteroaryl (examples include, but are not limited to, tyrosine,
3-chlorophenylalanine, 2,3-chloro-phenylalanine,
3-chloro-5-fluoro-phenylalanine,
2-chloro-6-fluoro-3-methyl-phenylalanine), or wherein the benzene
ring of the Phe analog can be replaced with another aryl group
(non-limiting examples include 1- and 2-naphthylalanine) or with a
heteroaryl group (non-limiting examples include pyridylalanine,
thienylalanine and furylalanine);
(d) is selected from the group consisting of Gly, tert-butyl-Gly,
Thr, Ser, Val and Asn;
(e) is selected from the group consisting of Leu, Ser, Thr, and
peptide bond isosteres such as, e.g., N-Me-Leu;
(f) is selected from the group consisting of Lys, Arg, Gly,
6-hydroxy-norleucine, citrulline (Cit), Gln and Ser;
(g) is selected from the group consisting of Leu, Asn, and peptide
bond isosteres such as, e.g., N-Me-Leu;
(h) is selected from the group consisting of Ile, tert-butyl-Gly
(tBu-Gly), Asn, and peptide bond isosteres such as, e.g.,
N-Me-Ile;
(i) is selected from the group consisting of Gly, Arg, Ser and
Asn;
(j) is selected from the group consisting of Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) and 2-amino-isobutyric acid
(Aib); and
(k) is selected from the group consisting of Leu, norleucine (Nle),
homoleucine (Hleu), Val, tert-butyl-Ala (tBu-Ala), Arg, Thr, Ser,
and peptide bond isosteres such as, e.g., N-Me-Leu.
To improve the delivery of the CNP variants to the target sites of
bone-related disorders (e.g., skeletal dysplasias), the CNP
variants can be attached (e.g., at the N-terminus and/or
C-terminus) to bone- or cartilage-targeting moieties. Non-limiting
examples of bone- or cartilage-targeting moieties include
bisphosphonates; hydroxyapatite; glucosamine; collagen (e.g.,
collagen type X); polyAsp; polyGlu; and amino acid sequences
derived from bone-targeting domains of bone proteins such as, e.g.,
osteocrin, osteopontin, osteocalcin, and sialoprotein.
In addition to being less susceptible to NEP cleavage, the CNP
variants potentially have reduced affinity to the NPR-C clearance
receptor, while retaining CNP functionality. Besides NEP-mediated
degradation, the half-life of CNP22 is influenced by the clearance
receptor, NPR-C, which shares 58% sequence homology with the
extracellular peptide-binding domain of NPR-B. CNP22 binds tightly
to not only NPR-B (7-30 pM affinity), but also NPR-C (11-140 pM)
(Bennett, B. D. et al., J. Biol. Chem., 266: 23060-67 (1991);
Koller K. J. & Goeddel, D. V., Circulation, 86: 1081-88 (1992);
Suga, S. et al., Endocrinology, 130: 229-39 (1992)). Even though
the NPR-B crystal structure has yet to be reported, sequence
homology as well as similarities between the NPR-C and NPR-A
crystal structures (He, X.-L. et al., Science, 293(5535): 1657-62
(2001); Ogawa, H. et al., J. Biol. Chem., 279(27): 28625-31 (2004);
He, X.-L., J. Mol. Biol., 361(4): 698-714 (2006)) suggest that
NPR-B likely assumes a similar overall structural fold.
Therefore, an NPR-B homology model was built based on
structure-based sequence alignment and crystallographic structures
of the following related systems: CNP bound to NPR-C, ANP bound to
NPR-A, and ANP bound to NPR-C (He, X.-L. et al., Science,
293(5535): 1657-62 (2001); Ogawa, H. et al., J. Biol. Chem.,
279(27): 28625-31 (2004); He, X.-L., J. Mol. Biol., 361(4): 698-714
(2006)). Based on observations that the receptor appears to
determine the bound peptide conformation, and that NPR-B most
closely resembles NPR-A with respect to primary structure and
functional properties, the NPR-B/CNP homology model was built with
the NPR-A/ANP crystal structure as a model. Published signaling
data of CNP variants (U.S. Pat. No. 5,434,133 and US Patent
Application Publication No. 2004/0138134 A1), and of functional ANP
variants that no longer bind to NPR-C (Cunningham, EMBO 13(11)
2508-15, 1994) were used to refine and interpret the NPR-B/CNP
model.
The present disclosure encompasses CNP variants designed for
improved NPR-B selectivity based on a homology-based structural
model of the NPR-B/CNP complex. Combining the experimental and
computational structure data of natriuretic peptides bound to the
various receptors with the published functional data, CNP variants
were generated that continue to bind to NPR-B, but can potentially
have reduced affinity to the NPR-C clearance receptor. For example,
NPR-C has a unique insertion in a loop structure in the
peptide-binding site, placing its loop residues closer to such
peptide residues as CNP Gly8 (or ANP Gly9), compared to respective
loop residues in NPR-A and NPR-B. Earlier studies indicated that
the G9T mutation in ANP contributes to reduce affinity to NPR-C,
thereby improving NPR-A selectivity (Cunningham, EMBO J., 13(11):
2508-15 (1994)). Accordingly, CNP variants were generated to
replace the corresponding Gly8 residue with a larger residue (Ser,
Val, Thr or Asn) to disrupt the CNP binding to NPR-C without
affecting its binding to NPR-B. Further, one or more mutations were
introduced at the C-terminal end of CNP, encompassing Gly15 to
Gly21, which is predicted to interact with receptor-specific
residues, based on the detailed structural analyses of the
receptor/peptide complexes. For example, a G19R mutation in CNP22
does not result in a significant loss of NPR-B signaling activity.
This mutation, however, cannot be modeled into the available
crystal structure of NPR-C/CNP without altering the conformations
of neighboring residues. These observations suggest that the G19R
mutation may selectively disrupt the binding of CNP to a particular
receptor, such as NPR-C.
In an embodiment, the CNP variants have substitution(s) at one or
more Gly sites at positions 1, 5, 8, 15, 19 and 21, to reduce
conformational flexibility and thereby increase receptor
specificity. Comparative analyses of crystal structures of ANP
bound to NPR-C and NPR-A (Ogawa, H. et al., J. Biol. Chem., 279:
28625-31 (2004); He, X.-L., J. Mol. Biol., 361: 698-714 (2006))
indicate that the conformational flexibility of ANP may play an
important role in determining the receptor selectivity.
In one embodiment, functional CNP variants with potentially reduced
affinity to NPR-C have one or more of the following amino acid
substitutions: G1R, G1E, G5R, G5Q, G5S, F7Y, G8T, G8S, G8V, G8N,
L9S, L9T, K10Cit, K10Q, K10S, 114N, G15R, G15S, G15N, G15Cit, S16Q,
M17V, M17N, G19S, G19R, G19N, L20V, L20R, L20T, L20S, G21S, G21T
and G21R. In an embodiment, the CNP variants have multipoint
substitutions at positions 1, 5, 7, 8, 9, 10, 14, 15, 16, 17, 19,
20 and/or 21, and may optionally have modifications at any of the
other positions in the peptide sequence of the variant.
In a further embodiment, the CNP variants described herein may be
conjugated to moieties, up to a total mass characterized by the
ranges described generally herein, e.g., from about 2.6 or 2.8 kDa
to about 6 or 7 kDa, at the N-terminus, the C-terminus and/or an
internal site, to facilitate bone/cartilage targeting, reduce NPR-C
and renal clearance, increase resistance to NEP degradation, and/or
improve CNP functionality. In one embodiment, the CNP variants are
not conjugated to a polymeric moiety at a site within the cyclic
region (corresponding to Cys6 to Cys22 of CNP22). Non-limiting
examples of polymeric or non-polymeric moieties that can be
conjugated to the CNP variants include synthetic bone-targeting
compounds such as, e.g., bisphosphonates; bone/cartilage targeting
peptide sequences such as, e.g., polyAsp and polyGlu; peptide
sequences derived from bone-targeting domains of bone proteins such
as, e.g., osteopontin, osteocalcin and sialoprotein; peptide
sequences derived from the functional domains of bone morphogenetic
proteins such as, e.g., BMP2, BMP3, BMP5, BMP7 and BMP8a; peptide
sequences derived from polypeptides of natriuretic origin such as,
e.g., NPPC, ANP and BNP; other natural polymeric or non-polymeric
moieties such as, e.g., carbohydrates, fatty acids and
phospholipids; biocompatible synthetic hydrophilic polymers such
as, e.g., PEG (or PEO); hydrophobic polymeric or non-polymeric
moieties such as, e.g., heptanoic acid and pentanoic acid; and
combinations thereof.
The CNP variants described herein can have substantially similar or
better functional activity than CNP22, e.g., with respect to
stimulation of cGMP production and signaling. In one embodiment,
the CNP variants in vitro or in vivo stimulate the production of at
least about 50% of the cGMP level produced under the same
concentration of wtCNP22 (e.g., 1 uM). In certain embodiments, the
CNP variants retain at least about 50%, 60%, 70%, 80%, 90%, 95% or
100% of the cGMP-stimulation activity of wild-type CNP22 in vitro
or in vivo. In another embodiment, the CNP variants have improved
cGMP-stimulation activity compared to CNP22. In certain
embodiments, the CNP variants in vitro or in vivo stimulate the
production of at least about 110%, 120%, 130%, 140%, 150%, 200%,
250%, 300%, 350%, 400%, 450%, 500% or more of the cGMP level
produced under the same concentration of wtCNP22 (e.g., 1 uM).
Optionally excluded from the present disclosure are any of the
natriuretic (e.g., CNP) peptides, fragments and variants
specifically disclosed, and any of the natriuretic (e.g., CNP)
peptides, fragments and variants actually produced, in any of the
prior publications referenced herein, including but not limited to,
U.S. Pat. Nos. 5,434,133, 6,034,231, 6,020,168, 6,743,425,
7,276,481, WO 94/20534, WO 02/047871, WO 2005/098490, WO
2004/047871, EP 0497368, EP 0466174, and Furuya et al., Biochem.
Biophys. Res. Comm. 183: 964-969 (1992)). All such documents are
incorporated by reference herein in their entirety.
In one embodiment, the present disclosure optionally excludes all
known wild-type CNP-53, wild-type CNP-22, wild-type CNP-17,
wild-type BNP, and wild-type ANP of human origin and non-human
origin. For example, in an embodiment the disclosure optionally
excludes human CNP-17, human CNP-22, chicken CNP-22 (corresponding
to hCNP-22(Leu9Val)), trout and eel CNP-22 (corresponding to
hCNP-22(Leu2Trp, Ser3Asn, Lys4Arg)), frog CNP22-I (corresponding to
hCNP-22(Leu2Tyr, Lys4Arg, Leu9Val, Ser16Ala, Met17Phe)), frog
CNP22-II (corresponding to hCNP-22(Leu2Thr, Ser16Ala)), human
CNP-53, and pig and rat CNP-53 (corresponding to hCNP-53(Gln17His,
Ala28Gly)). In another embodiment, the disclosure optionally
excludes fragments of NPPC, proCNP and CNP-53 which are produced by
proteolytic cleavage in vivo in humans and non-human animals. In
yet another embodiment, optionally excluded from the disclosure are
the following truncated fragments of wild-type human CNP-53:
CNP-50, CNP-46, CNP-44, CNP-39, CNP-30, CNP-29, CNP-28, CNP-27 and
CNP-26.
In a further embodiment, the present disclosure optionally excludes
CNP peptides and fragments thereof isolated or sought from the
shark species Triakis scyllia and Scyliorhinus canicula (see, e.g.,
M. Takano et al., Zool. Sci., 11: 451-454 (1994)), including:
TABLE-US-00020 (SEQ ID NO: 204)
RLLKDLSNNPLRFRGRSKKGPSRGCFGVKLDRIGAMSGLGC (CNP-41); (SEQ ID NO:
205) LKDLSNNPLRFRGRSKKGPSRGCFGVKLDRIGAMSGLGC (CRP-39); (SEQ ID NO:
206) KDLSNNPLRFRGRSKKGPSRGCFGVKLDRIGAMSGLGC (CNP-38); and (SEQ ID
NO: 207) GPSRGCFGVKLDRIGAMSGLGC (CNP-22).
In another embodiment, optionally excluded from the disclosure are
CNP peptides and fragments thereof isolated or sought from the
shark species Lamna ditropis (see, e.g., M. Takano et al., Zool.
Sci., 11: 451-454 (1994)), including:
TABLE-US-00021 (SEQ ID NO: 208)
RLLKDLSNNPLRFKGRSKKGPSRGCFGVKLDRIGAMSGLGC (CNP-41); (SEQ ID NO:
209) LKDLSNNPLRFKGRSKKGPSRGCFGVKLDRIGAMSGLGC (CRP-39); (SEQ ID NO:
210) KDLSNNPLRFKGRSKKGPSRGCFGVKLDRIGAMSGLGC (CNP-38); (SEQ ID NO:
211) FKGRSKKGPSRGCFGVKLDRIGAMSGLGC (CNP-29); and (SEQ ID NO: 212)
GPSRGCFGVKLDRIGAMSGLGC (CNP-22).
In still another embodiment, optionally excluded from the
disclosure are CNP peptides and fragments thereof isolated from the
shark species Squalus acanthias (see, e.g., M. Takano et al., Zool.
Sci., 11: 451-454 (1994)), including:
TABLE-US-00022 (SEQ ID NO: 213)
RLLQDLSNNPLRFKGRSKKGPSRSCFGLKLDRIGAMSGLGC (CNP-41); and (SEQ ID NO:
214) GPSRSCFGLKLDRIGAMSGLGC (CNP-22).
In a further embodiment, the present disclosure optionally excludes
CNP peptides isolated from medaka and puffer fish, designated
"CNP-1", "CNP-2", "CNP-3" and "CNP-4" in K. Inoue et al., Proc.
Nat. Acad. Sci., 100(17): 10079-10084 (2003):
TABLE-US-00023 (SEQ ID NO: 215) GWNRGCFGLKLDRIGSMSGLGC (medaka and
puffer fish CNP-1); (SEQ ID NO: 216) PMVAGGGCFGMKMDRIGSISGLGC
(medaka CNP-2); (SEQ ID NO: 217) GRSSMVGGRGCFGMKIDRIGSISGLGC
(puffer fish CNP-2); (SEQ ID NO: 218) GGMRSCFGVRLERIGSFSGLGC
(medaka CNP-3); (SEQ ID NO: 219) GGLASCFGVALARIGSFSGLGC (puffer
fish CNP-3); (SEQ ID NO: 220) GGSTSRSGCFGHKMDRIGTISGMGC (medaka
CNP-4); and (SEQ ID NO: 221) GGSSRSGCFGHKMDRIGTISGMGC (puffer fish
CNP-4).
In a still further embodiment, optionally excluded from the
disclosure are CNP-39 isolated from platypus venom and the CNP-22
fragment thereof, designated "ovCNP-39" and "ovCNP-39(18-39)" in G.
de Plater et al., Toxicon., 36(6): 847-857 (1998):
TABLE-US-00024 (SEQ ID NO: 222)
LLHDHPNPRKYKPANKKGLSKGCFGLKLDRIGSTSGLGC (ovCNP-39); and (SEQ ID NO:
223) GLSKGCFGLKLDRIGSTSGLGC (ovCNP-39 (18-39).
In another embodiment, the present disclosure optionally excludes
the following peptides as specifically disclosed in US
2007/0197434:
TABLE-US-00025 (SEQ ID NO: 224)
Gly-Leu-Ser-Lys-Gly-Cys-Phe-Gly-Leu-Lys-Leu-Asp-
Arg-Ile-Gly-Ala-Met-Ser-Gly-Leu-Gly-Cys; (SEQ ID NO: 225)
Gly-Leu-Ser-Lys-Gly-Cys-Phe-Gly-Leu-Lys-Leu-Asp-
Arg-Ile-Gly-Ser-Gln-Ser-Gly-Leu-Gly-Cys; (SEQ ID NO: 226)
Gly-Leu-Ser-Lys-Gly-Cys-Phe-Gly-Leu-Lys-Leu-Asp-
Arg-Ile-Gly-Ser-Ala-Ser-Gly-Leu-Gly-Cys; (SEQ ID NO: 227)
Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Leu-Lys-Leu-
Asp-Arg-Ile-Gly-Ser-Met-Ser-Gly-Leu-Gly-Cys; (SEQ ID NO: 228)
Gly-Leu-Ser-Lys-Gly-Cys-Phe-Gly-Leu-Lys-Leu-Asp-
Arg-Ile-Gly-Ser-Met-Ser-Gly-Leu-Gly-Cys-Asn-Ser- Phe-Arg-Tyr; and
(SEQ ID NO: 229) Cys-Phe-Gly-Leu-Lys-Leu-Asp-Arg-Ile-Gly-Ser-Gln-
Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr.
In yet another embodiment, the disclosure optionally excludes
peptides of SEQ ID NO:10, disclosed generically in US 2007/0197434,
wherein such peptides are CNP-17 variants having certain natural
amino acid substitution(s) at position(s) 4, 5, 6, 11, 12, 14
and/or 15. In still another embodiment, optionally excluded from
the disclosure are peptides corresponding to hCNP-53(Ser47Ala),
hCNP-53(Met48Gln), hCNP-53(Met48Ala), and
hCNP-53(C-term.)-Asn-Ser-Phe-Arg-Tyr.
In an embodiment, the present disclosure optionally excludes the
peptides of SEQ ID NOs 1-4 and 6-71 as specifically disclosed in
U.S. Pat. No. 7,276,481. In another embodiment, the disclosure
optionally excludes peptides of SEQ ID NO 5, disclosed generically
in U.S. Pat. No. 7,276,481, wherein such peptides are variants of
CNP17 having at least one natural amino acid substitution at Leu9,
Lys10, Leu11, Ser16, Met17, Gly19, and/or Leu20. In still another
embodiment, optionally excluded are CNP17 variants in which CNP17
or variants thereof contain N-Me-Phe7, or N-Me-Phe7 and N-Me-Leu11.
In a further embodiment, the disclosure optionally excludes CNP17
variants of SEQ ID NO 5, as disclosed in U.S. Pat. No. 7,276,481,
which are fused or conjugated to growth hormone (GH), insulin-like
growth factor 1 (IGF-1), or thyroid hormone (TH). In yet another
embodiment, optionally excluded are CNP22 variants in which CNP22
is fused to GH, IGF-1 or TH, or attached to GH, IGF-1 or TH via a
linker (e.g., a peptide linker). In still another embodiment,
optionally excluded are CNP17 variants in which CNP17 or variants
thereof are conjugated to biotin or fluorescein at the N-terminus
or the C-terminus.
In a further embodiment, the present disclosure optionally excludes
the peptides of Compound Nos. 1-27, and SEQ ID NOs 1-17, 22-24, 30,
31 and 40-42 as specifically disclosed in U.S. Pat. No. 5,434,133.
In another embodiment, the disclosure optionally excludes peptides
of SEQ ID NOs 18-21 and 25-29, disclosed generically in U.S. Pat.
No. 5,434,133. In still another embodiment, the disclosure
optionally excludes the peptides of SEQ ID NOs 1-4 and 9 as
specifically disclosed in WO 94/20534.
In some embodiments, however, the disclosure still encompasses
methods of use of the natriuretic (e.g., CNP) peptides, fragments
and variants optionally excluded herein, as well as pharmaceutical
compositions (including sterile pharmaceutical compositions)
comprising such natriuretic (e.g., CNP) peptides, fragments and
variants.
C. Synthesis and Purification of CNP Variants
In some embodiments, the CNP variants described herein are produced
by recombinant expression, using certain techniques known in the
art in certain embodiments. See, e.g., Sambrook, Fritsch &
Maniatis, Molecular Cloning: A Laboratory Manual, Second Edition.
Cold Spring Harbor Laboratory Press (Cold Spring Harbor, N.Y.
(1989)); DNA Cloning: A Practical Approach, Volumes I and II, D. N.
Glover, Ed. (1985); and Current Protocols in Molecular Biology,
John Wiley & Sons, Inc. (1994).
In certain embodiments, the CNP variants described herein are
produced by a recombinant process that comprises culturing in a
medium a host cell comprising a first polynucleotide encoding a CNP
variant polypeptide linked to a second polynucleotide encoding a
cleavable peptide or protein under conditions that result in
expression of a fusion polypeptide encoded by the polynucleotides,
wherein the fusion polypeptide comprises the CNP variant
polypeptide directly linked to the cleavable peptide or protein or
indirectly linked thereto via a linker. In some embodiments, the
host cell is transformed with an expression vector comprising the
polynucleotide encoding the CNP variant polypeptide linked to the
polynucleotide encoding the cleavable peptide or protein. In
certain embodiments, the fusion polypeptide is expressed as a
soluble protein or as an inclusion body. The expressed fusion
polypeptide can be isolated from the host cell or culture medium,
and the isolated fusion polypeptide can be contacted with a
cleaving agent to release the CNP variant.
Host cells used to produce CNP variants can be bacterial, yeast,
insect, non-mammalian vertebrate, or mammalian cells. Bacterial
cells include without limitation E. coli cell lines and strains.
Non-limiting examples of E. coli cell lines and strains include
BL21, BL21(DE3), BL21(DE3)pLysS, BL21(DE3)pGro7,
ArcticExpress(DE3), C41 [also called C41(DE3)], C43 [also called
C43(DE3)], Origami B(DE3), Origami B(DE3)pLysS, KRX, and
Tuner(DE3). In an embodiment, CNP variants and CNP fusion proteins
are produced using BL21(DE3) cells. Mammalian cells include, but
are not limited to, hamster, monkey, chimpanzee, dog, cat, bovine,
porcine, mouse, rat, rabbit, sheep and human cells. The host cells
can be immortalized cells (a cell line) or non-immortalized
(primary or secondary) cells and can be any of a wide variety of
cell types, such as, but not limited to, fibroblasts,
keratinocytes, epithelial cells (e.g., mammary epithelial cells,
intestinal epithelial cells), ovary cells (e.g., Chinese hamster
ovary or CHO cells), endothelial cells, glial cells, neural cells,
formed elements of the blood (e.g., lymphocytes, bone marrow
cells), chondrocytes and other bone-derived cells, and precursors
of these somatic cell types. Host cells containing the CNP variant
DNA or RNA are cultured under conditions appropriate for growth of
the cells, expression of the DNA or RNA and
identification/selection of cells expressing the CNP variant.
In some embodiments, the host cells are grown or cultured for a
time period at a temperature from about 10.degree. C. to about
40.degree. C., or from about 20.degree. C. to about 40.degree. C.,
or from about 30.degree. C. to about 40.degree. C. In certain
embodiments, the host cells are grown or cultured for a time period
at about 20.degree. C., 22.degree. C., 25.degree. C., 28.degree.
C., 30.degree. C., 35.degree. C. or 37.degree. C. In certain
embodiments, the host cells are grown or cultured for a time period
at about 35.degree. C. or 37.degree. C.
Recombinant polynucleotides encoding CNP variant polypeptides
(including CNP fusion proteins) are expressed in an expression
vector comprising a recombinant polynucleotide comprising
expression control sequences operatively linked to a nucleotide
sequence to be expressed. An expression vector comprises sufficient
cis-acting elements for expression; other elements for expression
can be supplied by the host cell or in vitro expression system.
Expression vectors include all those known in the art, including
without limitation cosmids, plasmids (e.g., naked or contained in
liposomes) and viruses that incorporate the recombinant
polynucleotide. The expression vector is inserted into an
appropriate host cell, via transformation or transfection, for
expression of the polynucleotide encoding the polypeptide (see,
e.g., Sambrook et al. (supra)).
Non-limiting examples of expression vectors contemplated for
production of CNP variants, including cleavable CNP fusion
proteins, include pJexpress, pJexpress401, pJexpress404, pET-15b,
pET-21a, pET-22b, pET-31b, pET-32a, pET-41a, pMAL, pMAL-c2X,
pQE-30, pET-SUMO, and pTYB11. Expression of particular constructs
can generate soluble CNP variants (including CNP fusion proteins)
or insoluble CNP variants (including CNP fusion proteins) in the
form of inclusion bodies.
In some embodiments, expression of the polynucleotide(s) encoding a
CNP variant or CNP fusion protein is enhanced using an isopropyl
.beta.-D-1-thiogalactopyranoside (IPTG)-inducible vector. In some
embodiments, the host cells are grown or cultured for a time period
at a temperature from about 10.degree. C. to about 40.degree. C.,
or from about 20.degree. C. to about 40.degree. C., or from about
30.degree. C. to about 40.degree. C., in the presence of IPTG. In
certain embodiments, the host cells are grown or cultured for a
time period at about 20.degree. C., 22.degree. C., 25.degree. C.,
28.degree. C., 30.degree. C., 35.degree. C. or 37.degree. C. in the
presence of IPTG. In certain embodiments, the host cells are grown
or cultured for a time period at about 35.degree. C. or 37.degree.
C. in the presence of 1 mM IPTG.
In further embodiments, the host cells are cultured with IPTG at a
concentration from about 0.4 mM to about 2 mM, or from about 0.4 mM
to about 1.5 mM, or from about 0.4 mM to about 1 mM. In certain
embodiments, the IPTG is at a concentration of about 0.4, 0.5, 0.6,
0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2
mM. In an embodiment, the concentration of IPTG is about 1 mM.
In certain embodiments, the CNP variants described herein are
recombinantly expressed as fusion proteins comprising a CNP variant
polypeptide and a cleavable carrier protein or cleavable tag (e.g.,
peptide tag), wherein the fusion protein comprises the CNP variant
polypeptide directly linked to the cleavable carrier protein or tag
or indirectly linked thereto via a linker. Use of a carrier protein
or tag facilitates, e.g., detection, isolation and/or purification
of the fusion protein. Cleavable carrier proteins and tags include,
but are not limited to, histidine (e.g., hexa-His) tags; human
transcription factor TAF12 (TAF12), TAF12 fragments, TAF12 histone
fold domain, mutants of TAF12 and fragments thereof, TAF12(C/A),
TAF12(D/E), TAF12(4D/4E), TAF12(6D/6E), TAF12(10D/10E), TAF12(C/A
& D/E), TAF12(C/A & 4D/4E), TAF12(C/A & 6D/6E),
TAF12(C/A & 10D/10E); ketosteroid isomerase (KSI);
maltose-binding protein (MBP); .beta.-galactosidase (.beta.-Gal);
glutathione-S-transferase (GST); thioredoxin (Trx); chitin binding
domain (CBD); BMP-2, BMP-2 mutants, BMP-2(C/A); SUMO; and mutants
and fragments thereof.
An expression construct can express a fusion protein comprising a
CNP variant and a carrier protein or tag. The tag can be an amino
acid sequence that confers a useful property to the fusion protein.
In one embodiment, the tag is a ligand-binding domain that can be
used to purify the fusion protein by applying the fusion protein to
separation media containing the ligand. For example, a fusion
protein comprising a glutathione-S-transferase (GST) domain can be
applied to a chromatographic column containing glutathione-linked
separation media. As another example, a fusion protein comprising
maltose-binding protein (MBP) as a tag can be applied to separation
media containing maltose. As a further example, a fusion protein
comprising a polyhistidine tag may be applied to a nickel column,
whereby chelation of the polyhistidine tag to the nickel column
facilitates purification of the fusion protein. In another
embodiment, the tag is a ligand. For example, a fusion protein can
comprise glutathione as a tag and be applied to a chromatographic
column containing glutathione-S-transferase-linked separation
media. Non-limiting examples of carrier proteins and tags for use
in fusion proteins include human transcription factor TAF12
(TAF12), ketosteroid isomerase (KSI), maltose-binding protein
(MBP), .beta.-galactosidase (.beta.-Gal), glutathione-S-transferase
(GST), thioredoxin (Trx), chitin-binding domain (CBD), BMP-2
mutation (BMPM), SUMO, CAT, TrpE, staphylococcal protein A,
streptococcal proteins, starch-binding protein, cellulose-binding
domain of endoglucanase A, cellulose-binding domain of exoglucanase
Cex, biotin-binding domain, recA, Flag, poly(His), poly(Arg),
poly(Asp), poly (Gln), poly(Phe), poly(Cys), green fluorescent
protein, red fluorescent protein, yellow fluorescent protein, cyan
fluorescent protein, biotin, avidin, streptavidin, antibody
epitopes, and mutants and fragments thereof.
To generate the target CNP variant, the carrier protein or tag can
be cleaved from the fusion protein by means of chemical cleavage,
protease cleavage, or protein self-cleavage. Exemplary chemical and
proteolytic cleavage agents (cleavage sites in parenthesis)
include, but are not limited to, formic acid (Asp-Pro), cyanogen
bromide (CNBr) (Met-X), hydroxylamine (Asn-Gly), Factor Xa (IEGR-X)
(SEQ ID NO: 230), Enterokinase (DDDDK-X) (SEQ ID NO: 231), ProTEV
(EXXYXQ-G) (SEQ ID NO: 232), and SUMO protease. Due to the nature
of the particular kinds of chemical cleavage, cleavage using formic
acid may generate Pro-CNP, CNBr may generate CNP having Met-to-Asn
substitution, and hydroxylamine may generate Gly-CNP.
Alternatively, chemical or protease cleavage may be avoided by
using particular constructs (e.g., pET-21a-CNP) that express CNP
variants not as fusion proteins. Expression of pET-21a-CNP may
produce Met-CNP. Or certain fusion proteins (e.g., those containing
intein-CBD) can undergo self-cleavage to generate CNP.
In further embodiments, a fusion protein comprises a cleavable
peptide linker between a CNP variant and a carrier protein or tag
(e.g., peptide tag). In certain embodiments, the cleavable peptide
linker is selected from the group consisting of Asp-Pro, Asn-Gly,
Met-X, Val-Asp-Asp-Arg (SEQ ID NO: 233), Gly-Ser-Asp-Arg (SEQ ID
NO: 234), Ile-Thr-Asp-Arg (SEQ ID NO: 235), Pro-Gly-Asp-Arg (SEQ ID
NO: 236), Ile-Glu-Gly-Arg-X (SEQ ID NO: 230), Asp-Asp-Asp-Asp-Lys-X
(SEQ ID NO: 231), Glu-X-X-Tyr-X-Gln-Gly (SEQ ID NO: 232),
Ala-Phe-Leu-Gly-Pro-Gly-Asp-Arg (SEQ ID NO: 237), and
MGSSHHHHHHSSGLVPRGSHTGD-DDDKHMD (pET-15b linker) (SEQ ID NO: 95),
where X denotes an amino acid. In some embodiments, the cleavable
peptide linker is cleaved by a cleaving agent selected from the
group consisting of palladium, cyanogen bromide (CNBr), formic
acid, hydroxylamine, clostripain, thrombin, chymotrypsin, trypsin,
trypsin-like proteases, carboxypeptidase, enterokinase
(enteropeptidase), Kex 2 protease, Omp T protease, Factor Xa
protease, subtilisin, proTEV, SUMO protease, V8 protease, HIV
protease, rhinovirus protease, furilisin protease, IgA proteases,
human Pace protease, collagenase, Nia protease, poliovirus 2Apro
protease, poliovirus 3C protease, genenase, furin, elastase,
Proteinase K, pepsin, rennin (chymosin), microbial aspartic
proteases, papain, calpain, chymopapain, ficin (ficain), bromelain
(bromelase), cathespisin B, caspases, thermolysin, Endoprotease
Arg-C, Endoprotease Glu-C, Endoprotease Lys-C, kallikrein, and
plasmin.
In certain embodiments, the cleavable carrier protein, tag (e.g.,
peptide tag) or peptide linker is cleaved using formic acid to
release the CNP variant from the fusion protein. In some
embodiments, the formic acid is at a concentration from about 1% to
about 20%, or from about 1% to about 15%, or from about 2% to about
15%, or from about 1% to about 10%, or from about 2% to about 10%,
or from about 1% to about 5%, or from about 2% to about 5%. In
certain embodiments, the formic acid is at a concentration of about
1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%.
In certain embodiments, the formic acid is at a concentration of
about 2%, 5% or 10%.
In further embodiments, cleavage of the CNP fusion protein in the
presence of formic acid is conducted at a temperature from about
20.degree. C. to about 80.degree. C., or from about 30.degree. C.
to about 75.degree. C., or from about 40.degree. C. to about
75.degree. C., or from about 50.degree. C. to about 75.degree. C.,
or from about 50.degree. C. to about 70.degree. C., or from about
55.degree. C. to about 70.degree. C., or from about 50.degree. C.
to about 60.degree. C. In some embodiments, cleavage in the
presence of formic acid is conducted at about 20.degree. C.,
22.degree. C., 25.degree. C., 30.degree. C., 35.degree. C.,
37.degree. C., 40.degree. C., 42.degree. C., 45.degree. C.,
50.degree. C., 55.degree. C., 60.degree. C., 65.degree. C.,
70.degree. C., 75.degree. C. or 80.degree. C. In certain
embodiments, cleavage in the presence of formic acid is conducted
at about 50.degree. C., 55.degree. C., 60.degree. C., 65.degree. C.
or 70.degree. C. In certain embodiments, cleavage in the presence
of formic acid is conducted at about 55.degree. C. or 70.degree.
C.
In additional embodiments, cleavage of the CNP fusion protein in
the presence of formic acid is performed for a time period from
about 3 hr to about 48 hr, or from about 5 hr to about 48 hr, or
from about 5 hr to about 36 hr, or from about 5 hr to about 24 hr,
or from about 5 hr to about 18 hr, or from about 20 hr to about 24
hr, or for about 6 hr to about 10 hr. In certain embodiments,
cleavage in the presence of formic acid is performed for about 5
hr, 6 hr, 12 hr, 15 hr, 18 hr, 20 hr or 24 hr.
In some embodiments, cleavage of the CNP fusion protein is
conducted in the presence of about 2%, 5% or 10% formic acid at
about 55.degree. C. for about 20 hr to about 36 hr, or at about
60.degree. C. for about 15 hr to about 24 hr, or at about
65.degree. C. for about 10 hr to about 21 hr, or at about
70.degree. C. for about 6 hr to about 18 hr. In certain
embodiments, cleavage of the CNP fusion protein is conducted in the
presence of about 2% formic acid at about 55.degree. C. for about
20 hr to about 24 or 36 hr, or at about 60.degree. C. for about 15
hr to about 24 hr, or at about 65.degree. C. for about 10 hr to
about 18 hr, or at about 70.degree. C. for about 6 hr to about 10
hr.
The present disclosure provides mild conditions for cleavage of CNP
fusion proteins using formic acid to afford high yields of CNP
variants. The conditions described herein for fusion protein
cleavage using formic acid are also suitable for cleavage of fusion
proteins comprising polypeptides or proteins other than CNP, where
the fusion proteins contain an Asp-Pro peptide bond.
In further embodiments, soluble CNP fusion proteins or CNP fusion
protein inclusion bodies are treated with a buffer and/or a
detergent prior to chemical cleavage (e.g., using formic acid) or
proteolytic cleavage of the CNP fusion protein. Non-limiting
examples of buffers include B-PER II; diluted B-PER II (e.g., 1/20
dilution); B-PER; B-PER phosphate buffer; buffers containing Tris
(e.g., 25 mM Tris, pH 7.5); buffers containing Tris and NaCl (e.g.,
25 mM Tris, 150 mM NaCl, pH 7.9); and PBS. In an embodiment, the
buffer is B-PER II. Non-limiting examples of detergents include
octyl-sucrose, Triton X-100, Tween-20, NP-40, and CA-630. The
detergent can be in a buffer (e.g., 1% detergent in 25 mM Tris
buffer, pH 7.5). In certain embodiments, the detergent is Triton
X-100 or CA-630.
It is understood that any of the methods and conditions described
above may be used in combination with any of the other methods and
conditions described above to generate a CNP variant disclosed
herein.
In other embodiments, the CNP variants described herein are
synthesized using a peptide synthesizer and purified according to
methods known in the art, e.g., according to the methods of
Atherton and Sheppard, Solid Phase Peptide Synthesis: a Practical
Approach, IRL Press (Oxford, England (1989)).
Peptides can be synthesized based on, e.g., the following peptide
sequence of CNP: G.sup.1LS(K or R)GC.sup.6F.sup.7G.sup.8L(K or R or
Nle or 6-OH-Nle)LDRIGSMSGLGC.sup.22.
Exemplary CNP variants include but are not limited to:
TABLE-US-00026 Analog A (GLSKGC(CH2NH)FGLKLDRIGSMSGLGC) (SEQ ID NO:
56) was made by converting the backbone "--C.dbd.O" group of
C.sup.6 to a "--CH.sub.2" group; Analog B
(GLSKGC(N--Me--Phe)GLKLDRIGSMSGLGC) (SEQ ID NO: 57) was made by
converting the backbone "--NH" group of F.sup.7 to an
"--N--CH.sub.3" group; Analog E (GLSKGC(D-Phe)GLKLDRIGSMSGLGC) (SEQ
ID NO: 136) was made using D-Phe at F.sup.7; Analog F
(GLSKGCF(tBu--Gly)LKLDRIGSMSGLGC) (SEQ ID NO: 58) was made using a
tertiary-butyl-Gly at G.sup.8; Analog G
(GLSKGC(3-Cl--Phe)GLKLDRIGSMSGLGC) (SEQ ID NO: 137) was made by
adding a chloride atom to a meta position of the phenyl ring of
F.sup.7 (similar variants can be generated by making ortho, meta
and/or para substitutions of the phenyl ring of Phe7 with Cl, F,
Br, OH and/or CH.sub.3); and Analog H
(GLSKGC[NHCH.sub.2CH(Ph)CO]GLKLDRIGSMSGLGC) (SEQ ID NO: 59) was
made using (.+-.)-3-(amino)-2- phenylpropionic acid at F.sup.7.
Examples of CNP variants having, e.g., amino acid extensions,
substitutions with natural or unnatural amino acids or peptide bond
isosteres, and/or conjugations to polymers or hydrophobic moieties,
include without limitation:
TABLE-US-00027 Analog J (SEQ ID NO: 91) C6-CH2-NH, N-Me-L9,
N-Me-L20 Analog K (SEQ ID NO: 92) N-Me-L9, N-Me-L20 Analog L (SEQ
ID NO: 93) N-Me-L9, N-Me-L11, N-Me-L20 Analog M (SEQ ID NO: 94)
N-Me-L9, N-Me-Lll Analog Z (SEQ ID NO: 95) K4R, F7Y Analog AA (SEQ
ID NO: 96) K4R, G8V Analog AB (SEQ ID NO: 97) K4R, GS Analog AC
(SEQ ID NO: 98) K4R, GT Analog AD (SEQ ID NO: 99) K4R, L9T Analog
AE (SEQ ID NO: 100) K4R, Gl5R Analog AF (SEQ ID NO: 101) K4R,
Gl5Cit Analog AG (SEQ ID NO: 102) K4R, M17V Analog AH (SEQ ID NO:
35) K4R Analog AJ (SEQ ID NO: 103) K4R, L2OV Analog AK (SEQ ID NO:
104) K4R, L20t-Bu-Ala Analog AT (SEQ ID NO: 105) GlE, K4E Analog AV
(SEQ ID NO: 106) GlE, K4E- pentanoic acid (attached at the
N-terminus) Analog AW (SEQ ID NO: 107) GlE, K4E - heptanoic acid
(attached at the N-terminus) Analog AX (SEQ ID NO: 2) CNP17 (delta
N-term) Analog AY (SEQ ID NO: 36) GANRR-CNP22(K4R) Analog AZ (SEQ
ID NO: 41) R-CNP22(K4R) Analog BB (SEQ ID NO: 108) G1E-heptanoic
acid (attached at the N-terminus) Analog BC (SEQ ID NO: 109)
G1E-pentanoic acid (attached at the N-terminus) Analog BF (SEQ ID
NO: 110) K4R, K10Cit Analog BG (SEQ ID NO: 111) K4R, K10Q Analog BH
(SEQ ID NO: 112) K4R, K10R Analog BJ (SEQ ID NO: 113) K4R, G15N
Analog BK (SEQ ID NO: 114) K4R, G15S Analog BL (SEQ ID NO: 60)
CNP-37 (SEQ ID NO: 4) CNP-53 Analog CA (SEQ ID NO: 61)
AAWARLLQEHPNA-CNP22 Analog CB (SEQ ID NO: 62) AAWARLLQEHPNAR-CNP22
Analog CC (SEQ ID NO: 63) DLRVDTKSRAAWAR-CNP22 Analog CD (SEQ ID
NO: 68) SPKMVQGSG-CNP17-KVLARH (N- and C-terminal BNP tails) Analog
CE (SEQ ID NO: 81) GERAFKAWAVARLSQ-CNP22 (HSA-CNP22) Analog CF (SEQ
ID NO: 79) GQPREPQVYTLPPS-CNP22 PEG(24K)-CNP22 PEG(20K)-CNP22
PEG(5K)-CNP22 PEG(2K)-CNP22 PEG(2K)-CNP17 (SEQ ID NO: 36)
PEG(1K)-GANRR-CNP22(K4R) PEG(1K)-CNP22
PE04-(PE012)3(branched)-CNP22 PE012-CNP22 (SEQ ID NO: 36)
PE012-GANRR-CNP22(K4R) (SEQ ID NO: 36) PE024-GANRR-CNP22(K4R); and
SEQ ID NOs: 1 to 6 and 34 to 144, and variants thereof that
comprise up to 1, 2, 3, 4, or 5 further modifications.
In one embodiment, the CNP variants are cyclized via formation of a
disulfide bond between Cys.sup.6 and Cys.sup.22. Cys.sup.6 can be a
cysteine analog such as, e.g., homocysteine or penicillamine. In a
further embodiment, the CNP variants can be cyclized by a covalent
bond formed head-to-tail, side chain-to-side chain, side
chain-to-head, or side chain-to-tail. In an embodiment, the
covalent bond is formed between an amino acid at or toward the
N-terminus and an amino acid at or toward the C-terminus of the
peptide (referred to as "terminal" amino acids in this context). In
another embodiment, the covalent bond is formed between the side
chains of the two terminal amino acids. In yet another embodiment,
the covalent bond is formed between the side chain of one terminal
amino acid and the terminal group of the other terminal amino acid,
or between the terminal groups of the two terminal amino acids.
Head-to-tail cyclization of the terminal amine to the terminal
carboxyl group can be carried out using a number of methods, e.g.,
using p-nitrophenyl ester, 2,4,5-trichlorophenyl ester,
pentafluorophenyl ester, the azide method, the mixed anhydride
method, HATU, a carbodimide (e.g., DIC, EDC or DCC) with a catalyst
such as HOBt, HONSu or HOAt, or on-resin cyclization.
In addition, the cyclic structure can be formed via a bridging
group involving the side chains of amino acid residues of the CNP
variant and/or the terminal amino acid residues. A bridging group
is a chemical moiety that allows cyclization of two portions of the
peptide. Non-limiting examples of bridging groups include amides,
thioethers, thioesters, disulfides, ureas, carbamates,
sulfonamides, and the like. A variety of methods are known in the
art for incorporation of units having such bridging groups. For
example, a lactam bridge (i.e., a cyclic amide) can be formed
between the N-terminal amino group or an amino group on a side
chain and the C-terminal carboxylic acid or a carboxyl group on a
side chain, e.g., the side chain of lysine or ornithine and the
side chain of glutamic acid or aspartic acid. A thioester can be
formed between the C-terminal carboxyl group or a carboxyl group on
a side chain and the thiol group on the side chain of cysteine or a
cysteine analog.
Alternatively, a cross link can be formed by incorporating a
lanthionine (thio-dialanine) residue to link alanine residues that
are covalently bonded together by a thioether bond. In another
method, a cross-linking agent, such as a dicarboxylic acid (e.g.,
suberic acid (octanedioic acid)), can link the functional groups of
amino acid side chains, such as free amino, hydroxyl, and thiol
groups.
Enzyme-catalyzed cyclization can also be used. For example, it has
been reported that the thioesterase domain of tyrocidine synthetase
can be used to cyclize a thioester precursor, a subtilisin mutant
can be utilized to cyclize peptide glycolate phenylalanylamide
esters, and the antibody ligase 16G3 can be employed to cyclize a
p-nitrophenylester. For a review of peptide cyclization, see
Davies, J. Peptide Sci., 9: 471-501 (2003), incorporated herein by
reference in its entirety.
In certain embodiments, the final cyclized product has a purity of
at least about 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at
least about 99%.
D. Chemically Modified CNP Variants
Chemical modification of CNP22 or variants thereof can potentially
impart advantageous properties to the modified CNP peptides, such
as increased stability and half-life and reduced immunogenicity
(for a general discussion of chemical modification of therapeutic
proteins, see Pharmazie, 57(1): 5-29 (2002)). For example,
attaching natural or synthetic, polymeric or non-polymeric moieties
(e.g., PEG) to CNP peptides, to increase the total mass of the CNP
peptides to the ranges described generally herein, e.g., a range
from about 2.6 or 2.8 kDa to about 6 or 7 kDa, can reduce the
susceptibility of the modified peptides to in vivo cleavage by
exopeptidases and/or endopeptidases (e.g., NEP). In addition to
PEGylation, glycosylation and other chemical derivatization
procedures, e.g., modification by phosphorylation, amidation,
carboxylation, acetylation, methylation, and creation of
acid-addition salts, amides, esters and N-acyl derivatives, may
also mask potentially immunogenic regions and/or proteolytically
sensitive regions (Science, 303: 480-482 (2004)).
Examples of chemical modifications include, without limitation, the
polymer addition method of Bednarsaki and the cross-linking method
of Altus Corporation for improving stability and protease
resistance and reducing immunogenicity. Bednarsaki showed that
polymer addition can improve protein temperature stability (J. Am.
Chem. Soc., 114(1): 378-380 (1992)), and Altus Corporation found
that glutaraldehyde cross-linking can improve enzyme stability.
Chemical modification of polypeptides can be performed in a
non-specific fashion (leading to mixtures of derivatized species)
or in a site-specific fashion (e.g., based on wild-type
macromolecule reactivity-directed derivatization and/or
site-selective modification using a combination of site-directed
mutagenesis and chemical modification) or, alternatively, using
expressed protein ligation methods (Curr. Opin. Biotechnol., 13(4):
297-303 (2002)).
Pegylated CNP Variants
In one embodiment, for increased stability (e.g., resistance to NEP
degradation), CNP22 or variants thereof (including those having
amino acid additions, substitutions and/or deletions) are
conjugated to hydrophilic, natural or synthetic polymers, to
increase the total mass of the modified CNP peptides to a range
from about 2.6 kDa or 2.8 kDa to about 4, 5, 6, 7 or higher kDa. In
certain embodiments, the added hydrophilic polymers have a total
mass of about 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6,
2.8, 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8, or about 5
kDa.
In an embodiment, the hydrophilic polymers are water-soluble so
that the CNP peptides conjugated thereto do not precipitate out in
an aqueous (e.g., physiological) environment. Further, the
hydrophilic polymers are biocompatible, i.e., do not cause injury,
toxicity or an immunological reaction in vivo.
The hydrophilic polymers can be branched or unbranched. In one
embodiment, the hydrophilic polymers are not branched.
Various sites of conjugation of CNP22 or variants thereof to a
hydrophilic polymer are possible, including but not limited to: (1)
only at the N-terminus; (2) only at the C-terminus; (3) only at an
internal site (e.g., Lys4); (4) at both the N-terminus and the
C-terminus; (5) at the N-terminus and an internal site; and (6) at
the C-terminus and an internal site. In one embodiment, CNP22 or
variants thereof are conjugated to a hydrophilic polymer only at
the N-terminus. In another embodiment, conjugation is only at an
internal site (e.g., Lys4). In yet another embodiment, conjugation
is at the N-terminus and an internal site (e.g., Lys4). In still
another embodiment, for better functionality the CNP peptides are
not conjugated to a hydrophilic polymer at a site (e.g., Lys10)
within the cyclic domain (corresponding to Cys6 to Cys22 of CNP22).
If conjugation to a hydrophilic polymer is based on bond formation
with a reactive primary amino group on the CNP peptide, conjugation
at an internal site (e.g., Lys4 and/or Lys10) can be prevented by
substitution of Lys4 and/or Lys10 with a natural or unnatural amino
acid or peptidomimetic that does not contain a reactive primary
amino group on a side chain, such as, e.g., Gly, Ser, Arg, Asn,
Gln, Asp, Glu or citrulline (Cit). In a particular embodiment, Lys4
and/or Lys10 are replaced with Arg. In another embodiment, Lys10 is
not replaced with Arg.
Non-limiting examples of hydrophilic polymers include polymers
formed from carboxylic acid-bearing monomers (e.g., methacrylic
acid (MA) and acrylic acid (AA)), polyvinyl alcohols, polymers
formed from hydroxyl-bearing monomers (e.g., hydroxyethyl
methacrylate (HEMA), hydroxypropyl methacrylate (HPMA),
hydroxypropyl methacrylamide, and 3-trimethylsilylpropyl
methacrylate (TMSPMA)), polyalkylene oxides, polyoxyethylated
polyols (e.g., glycerol), poly(ethylene glycol) (PEG),
poly(propylene glycol), mono-C.sub.1-C.sub.10 alkoxy-PEGs (e.g.,
monomethoxy-PEG), tresyl monomethoxy-PEG, aryloxy-PEGs, PEG
acrylate (PEGA), PEG methacrylate, PEG propionaldehyde,
bis-succinimidyl carbonate PEG, copolymers of
2-methacryloyloxyethyl-phosphorylcholine (MPC) and N-vinyl
pyrrolidone (VP), hydroxy functional poly(N-vinyl pyrrolidone)
(PVP), SIS-PEG (SIS is polystyrene-polyisobutylene-polystyrene
block copolymer), polystyrene-PEG, polyisobutylene-PEG, PCL-PEG
(PCL is polycaprolactone), PLA-PEG (PLA is polylactic acid),
PMMA-PEG (PMMA is poly(methyl methacrylate)), PDMS-PEG (PDMS is
poly-dimethyloxanone), PVDF-PEG (PVDF is polyvinylidene fluoride),
PLURONIC.TM. surfactants (polypropylene oxide-co-polyethylene
glycol), poly(tetramethylene glycol), poly (L-lysine-g-ethylene
glycol) (PLL-g-PEG), poly(L-lysine-g-hyaluronic acid) (PLL-g-HA),
poly(L-lysine-g-phosphoryl choline) (PLL-g-PC),
poly(L-lysine-g-vinyl pyrrolidone) (PLL-g-PVP),
poly(ethylimine-g-ethylene glycol) (PEI-g-PEG),
poly(ethylimine-g-hyaluronic acid) (PEI-g-HA), poly
(ethylimine-g-phosphoryl choline) (PEI-g-PC),
poly(ethylimine-g-vinyl pyrrolidone) (PEI-g-PVP), PLL-co-HA,
PLL-co-PC, PLL-co-PVP, PEI-co-PEG, PEI-co-HA, PEI-co-PC,
PEI-co-PVP, cellulose and derivatives thereof (e.g., hydroxyethyl
cellulose), dextran, dextrins, hyaluronic acid and derivatives
thereof (e.g., sodium hyaluronate), elastin, chitosan, acrylic
sulfate, acrylic sulfonate, acrylic sulfamate, methacrylic sulfate,
methacrylic sulfonate, methacrylic sulfamate, polymers and
copolymers thereof, and polymers and copolymers of combinations
thereof.
In a particular embodiment, the hydrophilic polymer is
poly(ethylene glycol) (PEG), also called poly(ethylene oxide)
(PEO). As used herein, the term "PEG" or "PEO" encompasses all the
forms of PEG, branched and unbranched, which can be used to
derivatize polypeptides, including without limitation
mono-(C.sub.1-C.sub.10) alkoxy-PEGs and aryloxy-PEGs.
In one embodiment, the PEG-CNP conjugates comprise a PEG (or PEO)
polymer of the formula (CH.sub.2CH.sub.2O).sub.n, wherein n is an
integer from about 6 to about 100, and the PEG polymer is from
about 0.3 kDa to about 5 kDa. In another embodiment, n is an
integer from about 12 to about 50, and the PEG polymer is from
about 0.6 kDa to about 2.5 kDa. In yet another embodiment, n is
from about 12 to about 24, and the PEG polymer is from about 0.6
kDa to about 1.2 kDa. In a further embodiment, the terminal
hydroxyl group of the PEG polymer is capped with a non-reactive
group. In a particular embodiment, the end-capping group is an
alkyl group, e.g., a lower alkyl group such as methyl, so that the
PEG polymer terminates in an alkoxy group. In an embodiment, the
PEG polymer is not branched. In another embodiment, CNP22 or
variants thereof are conjugated to a PEG polymer only at the
N-terminus.
PEGs and PEOs potentially include molecules with a distribution of
molecular weights, i.e., they are potentially poly-dispersed,
depending on the manner in which they are prepared. The size/mass
distribution of a polymeric preparation can be characterized
statistically by its weight average molecular weight (M.sub.w) and
its number average molecular weight (M.sub.n), the ratio of which
is called the polydispersity index (M.sub.w/M.sub.n). M.sub.w and
M.sub.n can be measured by mass spectroscopy. PEG-CNP variants
conjugated to a PEG moiety larger than 1.5 kDa may exhibit a range
of molecular weights due to the polydispersed nature of the parent
PEG molecule. For example, in the case of mPEG2K (Sunbright
ME-020HS, NOF Co.), the molecular masses of the PEG molecules are
distributed over a range from about 1.5 kDa to about 3 kDa, with a
polydispersity index of 1.036. By contrast, the PEGs conjugated to
CNP22 or variants thereof using MS (PEG).sub.n reagents (n=4, 8, 12
or 24, denoted as, e.g., "PEO12" or "PEO24") from Pierce
Biotechnology (Rockford, Ill.) are monodispersed, having discrete
chain length and defined molecular weight.
Methods for generating polypeptides comprising a PEG moiety are
known in the art (see, e.g., U.S. Pat. No. 5,824,784). Methods for
preparing PEGylated CNP peptides generally comprise the steps of
(a) reacting CNP22 or a variant thereof with a PEGylation reagent
under conditions suitable for attaching PEG to the CNP peptide
(e.g., at the N-terminus), and (b) obtaining the reaction
product(s). Because PEGylating a CNP peptide might significantly
alter its binding to NPR-B, depending on the size of the PEG moiety
and the location of PEGylation, different kinds of PEG and
PEGylation reaction conditions can be explored. The chemistry that
can be used for PEGylation of a CNP peptide includes acylation of
reactive primary amine(s) of the peptide using the NHS-ester of
methoxy-PEG
(O--[(N-Succinimidyloxycarbonyl)-methyl]-O'-methylpolyethylene
glycol). Acylation with methoxy-PEG-NHS or methoxy-PEG-SPA results
in an amide linkage that eliminates any charge of the original
primary amine. PEG-CNP peptides designated with the symbol "PEO12"
or "PEO24", as well as those designated with the symbol "PEG1K",
"PEG2K", "PEG5K" or "PEG20K", are PEGylated via reaction of a
primary amino group on the peptide with an NHS ester-activated,
methoxy-end capped PEG reagent. PEG-CNP variants can also be
prepared by other methods, e.g., via reductive amination involving
a primary amino group on the peptide and a PEG aldehyde, such as,
e.g., PEG-propionaldehyde, or mono-C.sub.1-C.sub.10 alkoxy or
aryloxy derivatives thereof (see U.S. Pat. No. 5,252,714).
Unlike ribosome protein synthesis, synthetic peptide synthesis
proceeds from the C-terminus to the N-terminus. Accordingly,
Boc-PEG (containing tert-butyloxycarbonyl (Boc)) is one method to
attach PEG to the C-terminus of a peptide (R. B. Merrifield, J. Am.
Chem. Soc., 85(14): 2149-2154 (1963)). Alternatively, Fmoc
(fluorenylmethoxycarbonyl) chemistry can be employed (E. Atherton
and R. C. Sheppard, Solid Phase Peptide Synthesis: a Practical
Approach, IRL Press (Oxford, England (1989)).
The present methods for preparing PEG-CNP variants provide a
substantially homogenous mixture of polymer-protein conjugates.
After purification, discrete PEG-CNP preparations are sufficiently
pure for in vitro and in vivo testing of biological properties. As
demonstrated herein, certain PEG-CNP variants exhibit reduced
susceptibility to NEP cleavage and substantially similar or better
functionality (e.g., stimulation of cGMP production).
As described herein, PEGylation reactions of CNP22 or variants
thereof, using appropriate PEGylation reagent/CNP peptide ratios
and reaction conditions, provide PEG-CNP derivatives. The nature
and extent of PEGylation can be determined using, e.g., PAGE and
HPLC analysis. In certain embodiments, at least about 50%, 60%,
70%, 80%, 90%, 95% or 99% of CNP22 or variants thereof are
mono-PEGylated at the N-terminus. To optimize the beneficial
effects of PEGylation on the biological properties of a CNP
peptide, the polymer length, conformation (e.g., branched or
linear), and/or functionalization (e.g., adding a negatively
charged group) of a PEG moiety can be varied. PEGylated CNP
variants are tested for NEP resistance, pharmacokinetics and
bioactivity (e.g., the ability to bind to NPR-B and stimulate the
generation of cGMP). PEGylated CNP variants that show improved NEP
resistance and at least about 50% of the cGMP-stimulating activity
of CNP22 can be further tested, e.g., in vitro in a rat
chondrosarcoma cell-based achondroplasia model and in vivo in a
murine achondroplasia animal model.
E. Methods of Using CNP Variants, Pharmaceutical Compositions of
CNP Variants, and Routes of Administration
Methods of Using CNP Variants
Bone-Related Disorders
Fibroblast growth factors (FGFs) play important roles in bone
formation, and mutations in FGF receptor genes (FGFR 1, 2 and 3)
give rise to a variety of inherited skeletal malformations (Curr.
Biol., 5: 500-507 (1995)). In particular, activating mutations in
FGFR-3 are responsible for disorders of the long bones, including
achondroplasia, the most common form of human genetic dwarfism
(Nature, 371: 252-254 (1994); Cell, 78: 335-342 (1994)), the milder
disorder hypochondroplasia (Ann. N.Y. Acad. Sci., 785: 182-187
(1996)), and the more severe and neonatal lethal thanatophoric
dysplasia (TD) types I and II (Hum. Mol. Genet., 5: 509-512 (1996);
Nat. Genet., 9: 321-328 (1995)). Mouse models over-expressing
FGF-2, and consequentially activating FGFR-3, show shortened long
bones and macrocephaly (Mol. Biol. Cell, 6: 1861-73 (1995)).
Consistent with this model, mice deficient in FGFR-3 show
remarkable skeletal overgrowth with wider growth plates (Nature
Genet., 12: 390-397 (1996)).
Complementary experiments with CNP, NPR-B and NPR-C suggest a link
between the peptide ligand, the corresponding receptors, and bone
growth. Activation of NPR-B by elevated plasma concentrations of
CNP in transgenic mice causes skeletal overgrowth (Nat. Med., 10:
80-86 (2004)) histologically similar to that of the growth plate
cartilage of FGFR-3 knockout mice (Nat. Genet., 4: 390-397 (1996)).
In NPR-C knockout mice, NPR-C-mediated clearance of CNP should be
eliminated; consistent with this prediction, the knockout animals
show elongated long bones and elongated vertebrae with kyphosis
(Proc. Natl. Acad. Sci. USA 96: 7403-08 (1999)). Conversely, CNP
knockout mice are dwarfed with shorter long bones and vertebrae, a
phenotype histologically similar to that of achondroplasia, and
have increased mortality as a result of malocclusion and pulmonary
restriction from the small rib cage (Proc. Natl. Acad. Sci. USA,
98: 4016-4021 (2001)). Consistent with the proposed role of CNP as
an activator of NPR-B, the NPR-B knockout mouse has the same
dwarfed skeletal phenotype and increased mortality as the CNP
knockout mouse (Proc. Natl. Acad. Sci. USA, 101: 17300-05 (2004)).
Furthermore, in a mouse model of achondroplasia with activated
FGFR-3 in the cartilage, targeted overexpression of CNP in
chondrocytes counteracts dwarfism (Yasoda et al., Nat. Med., 10:
80-86 (2004)). Additionally, CNP has been show to play a role in
regulating endochondral bone growth and chondrocyte activity,
including but not limited to chondrocyte proliferation and
differentiation, inhibition of the mitogen activated protein (MAP)
kinase/MEK (Raf-1) kinase signaling pathway, and promotion of
endochondral ossification (Yasoda et al., Nat. Med., 10: 80-86
(2004)). These results suggest that activation of the CNP/NPR-B
system is a potential therapeutic strategy for treatment of human
achondroplasia.
By stimulating matrix production, proliferation and differentiation
of chondrocytes and increasing long bone growth, the CNP variants
of the disclosure are useful for treating mammals, including
humans, suffering from a bone-related disorder, such as a skeletal
dysplasia. Non-limiting examples of CNP-responsive bone-related
disorders and skeletal dysplasias include achondroplasia,
hypochondroplasia, short stature, dwarfism, osteochondrodysplasias,
thanatophoric dysplasia, osteogenesis imperfecta, achondrogenesis,
chondrodysplasia punctata, homozygous achondroplasia,
chondrodysplasia punctata, camptomelic dysplasia, congenital lethal
hypophosphatasia, perinatal lethal type of osteogenesis imperfecta,
short-rib polydactyly syndromes, hypochondroplasia, rhizomelic type
of chondrodysplasia punctata, Jansen-type metaphyseal dysplasia,
spondyloepiphyseal dysplasia congenita, atelosteogenesis,
diastrophic dysplasia, congenital short femur, Langer-type
mesomelic dysplasia, Nievergelt-type mesomelic dysplasia, Robinow
syndrome, Reinhardt syndrome, acrodysostosis, peripheral
dysostosis, Kniest dysplasia, fibrochondrogenesis, Roberts
syndrome, acromesomelic dysplasia, micromelia, Morquio syndrome,
Kniest syndrome, metatrophic dysplasia, and spondyloepimetaphyseal
dysplasia. Further, the CNP variants are useful as an adjunct or
alternative to growth hormone for treating idiopathic short stature
and other skeletal dysplasias.
In addition, the CNP variants are useful for treating other
bone-related conditions and disorders, such as rickets,
hypophosphatemic rickets [including X-linked hypophosphatemic
rickets (also called vitamin D-resistant rickets) and autosomal
dominant hypophosphatemic rickets], and osteomalacia [including
tumor-induced osteomalacia (also called oncogenic osteomalacia or
oncogenic hypophosphatemic osteomalacia)].
The CNP variants of the disclosure can also be used to treat
osteoarthritis. Osteoarthritis is a degenerative disease of the
articular cartilage and occurs frequently in the elderly.
Osteoarthritis involves destruction of the cartilage and
proliferative change in the bone and cartilage resulting from
degeneration of articular components, with the change resulting in
a secondary arthritis (e.g., synovitis). The extracellular matrix
proteins, which are the functional entity of the cartilage, are
reduced, and the number of chondrocytes decreases in osteoarthritis
(Arth. Rheum. 46(8): 1986-1996 (2002)). By promoting the matrix
production, growth and differentiation of chondrocytes, the CNP
variants are useful for countering the undesired effects of FGF-2
and increasing matrix synthesis in subjects suffering from
arthritis, including osteoarthritis, thereby treating arthritis,
including osteoarthritis.
Vascular Smooth Muscle Disorders
CNP and other vasoactive peptides (including ANP, BNP and
urodilatin) have vasodilator and diuretic properties and play an
important role in cardiovascular homeostasis (J. Cardiovasc.
Pharmacol., 117: 1600-06 (1998); Kidney Int., 49: 1732-37 (1996);
Am. J. Physiol., 275: H1826-1833 (1998)). CNP is widely distributed
in the cardiovascular system, especially in high concentration in
vascular endothelial cells (J. Cardiovasc. Pharmacol., 117: 1600-06
(1998)). CNP is a potent relaxant of vascular smooth muscle,
particularly in the coronary circulation (Biochem. Biophys. Res.
Commun., 205: 765-771 (1994)), and is an inhibitor of smooth muscle
cell proliferation (Biochem. Biophys. Res. Commun., 177: 927-931
(1991)). Although the vasodilator effect of CNP is less potent than
that of ANP (about 1:100) (Hypertens. Res., 21: 7-13 (1998); Am. J.
Physiol., 275: L645-L652 (1998)), CNP mRNA is increased in response
to shear stress (FEBS Lett., 373: 108-110 (1995)) and plasma levels
of CNP are elevated in inflammatory cardiovascular pathologies
(Biochem. Biophys. Res. Commun., 198: 1177-1182 (1994)). CNP has
been shown to suppress inflammation through inhibition of
macrophage infiltration in injured carotid arteries of rabbits
(Circ. Res., 91: 1063-1069 (2002)) and to directly inhibit cardiac
fibroblast proliferation through an NPR-B/cGMP-dependent pathway
(Endocrinology, 144: 2279-2284 (2003)).
The cardiovascular actions of CNP are mediated via activation of
the NPR subtypes, NPR-B and NPR-C (Endocrinology, 130: 229-239
(1992)), the latter accounting for 95% of NPRs expressed in vivo
(Science, 293: 1657-1662 (2001)). The CNP/NPR-B pathway leads to
elevation of cGMP, a well-established secondary messenger in the
cardiovascular system. NPR-C's 37-amino acid portion from the
C-terminus has a consensus sequence that interacts with the
heterotrimeric G protein G.sub.i (J. Biol. Chem., 274: 17587-17592
(1999)), which has been shown to regulate adenylate cyclase and
phospholipase C activity (J. Biol. Chem., 276: 22064-70 (2001); Am.
J. Physiol., 278: G974-980 (2000); J. Biol. Chem., 271: 19324-19329
(1996)). CNP mediates smooth muscle hyperpolarization and
relaxation via activation of NPR-C and the opening of a G
protein-regulated, inwardly rectifying K.sup.+ channels (Proc.
Natl. Acad. Sci. USA, 100: 1426-1431 (2003)). Likewise, CNP has
important anti-proliferative effects in cardiac fibroblasts and,
through interaction with NPR-C, regulates local blood flow and
systemic blood pressure by hyperpolarizing smooth muscle cells (R.
Rose and W. Giles, J. Physiol. 586: 353-366 (2008)).
By binding to NPR-B on vascular smooth muscle cells, CNP22
stimulates the production of cGMP, which acts as an intracellular
secondary messenger to cause ultimately the relaxation of blood
vessels. Based on the hypotensive actions of CNP, the CNP variants
of the disclosure are useful for treating hypertension, congestive
heart failure, cardiac edema, nephredema, hepatic edema, acute and
chronic renal insufficiency, and so on. In addition, activation of
cGMP signaling suppresses the growth of vascular smooth muscle
cells. Accordingly, the CNP variants of the disclosure can be used
to treat conditions or diseases caused by the abnormal growth of
vascular smooth muscle cells, including but not limited to
restenosis and arteriosclerosis.
The studies described above suggest that CNP may be a potential
therapeutic candidate for vascular smooth muscle relaxation and
remodeling. Pharmacological effects of CNP concerning certain
disorders have been attributed, in part, to vasoprotective effects
rather than to vasodilator activity (Am. J. Respir. Crit. Care
Med., 170: 1204-1211 (2004)). Therefore, the CNP variants of the
present disclosure are useful for treating conditions, e.g.,
vascular smooth muscle disorders, in which CNP may have a
vasoprotective effect, including without limitation inducing smooth
muscle relaxation and inhibiting infiltration of macrophages into
cardiac tissue. In one embodiment, the CNP variants are used to
treat heart failure, including but not limited to acute
decompensated heart failure and acute congestive heart failure. In
another embodiment, the CNP variants are used to treat asthma,
cardiomyopathy, and restenosis of coronary arteries (by increasing
smooth muscle cell relaxation and decreasing proliferation of
smooth muscle cells).
Pharmaceutical Compositions of CNP Variants
In additional embodiments, the disclosure provides pharmaceutical
compositions comprising a CNP variant, and one or more
pharmaceutically acceptable excipients, carriers and/or diluents.
In certain embodiments, the compositions further comprise one or
more other biologically active agents (e.g., inhibitors of
proteases, receptor tyrosine kinases, and/or the clearance receptor
NPR-C).
In some embodiments, the compositions comprise the desired CNP
variant in at least about 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98% or 99% purity. In certain embodiments, the compositions contain
less than about 10%, 5%, 4%, 3%, 2%, 1% or 0.5% of macromolecular
contaminants, such as other mammalian (e.g., human) proteins and
other CNP variants.
Non-limiting examples of excipients, carriers and diluents include
vehicles, liquids, buffers, isotonicity agents, additives,
stabilizers, preservatives, solubilizers, surfactants, emulsifiers,
wetting agents, adjuvants, and so on. The compositions can contain
liquids (e.g., water, ethanol); diluents of various buffer content
(e.g., Tris-HCl, phosphate, acetate buffers, citrate buffers), pH
and ionic strength; detergents and solubilizing agents (e.g.,
Polysorbate 20, Polysorbate 80); anti-oxidants (e.g., methionine,
ascorbic acid, sodium metabisulfite); preservatives (e.g.,
Thimerosol, benzyl alcohol, m-cresol); and bulking substances
(e.g., lactose, mannitol, sucrose). The use of excipients, diluents
and carriers in the formulation of pharmaceutical compositions is
known in the art; see, e.g., Remington's Pharmaceutical Sciences,
18.sup.th Edition, pages 1435-1712, Mack Publishing Co. (Easton,
Pa. (1990)), which is incorporated herein by reference in its
entirety.
For example, carriers include without limitation diluents, vehicles
and adjuvants, as well as implant carriers, and inert, non-toxic
solid or liquid fillers and encapsulating materials that do not
react with the active ingredient(s). Non-limiting examples of
carriers include phosphate buffered saline, physiological saline,
water, and emulsions (e.g., oil/water emulsions). A carrier can be
a solvent or dispersing medium containing, e.g., ethanol, a polyol
(e.g., glycerol, propylene glycol, liquid polyethylene glycol, and
the like), a vegetable oil, and mixtures thereof.
In some embodiments, the compositions are liquid formulations. In
certain embodiments, the formulations comprise a CNP variant in a
concentration range from about 0.1 mg/ml to about 20 mg/ml, or from
about 0.5 mg/ml to about 20 mg/ml, or from about 1 mg/ml to about
20 mg/ml, or from about 0.1 mg/ml to about 10 mg/ml, or from about
0.5 mg/ml to about 10 mg/ml, or from about 1 mg/ml to about 10
mg/ml.
In further embodiments, the compositions comprise a buffer solution
or buffering agent to maintain the pH of a CNP-containing solution
or suspension within a desired range. Non-limiting examples of
buffer solutions include phosphate buffered saline, Tris buffered
saline, and Hank's buffered saline. Buffering agents include
without limitation sodium acetate, sodium phosphate, and sodium
citrate. Mixtures of buffering agents can also be used. In certain
embodiments, the buffering agent is acetic acid/acetate or citric
acid/citrate. The amount of buffering agent suitable in a
composition depends in part on the particular buffer used and the
desired pH of the solution or suspension. For example, acetate is a
more efficient pH buffer at pH 5 than pH 6, so less acetate may be
used in a solution at pH 5 than at pH 6. In some embodiments, the
buffering agent has a concentration of about 10 mM.+-.5 mM. In
certain embodiments, the pH of a composition is from about pH 3 to
about pH 7.5, or from about pH 3.5 to about pH 7, or from about pH
3.5 to about pH 6.5, or from about pH 4 to about pH 6, or from
about pH 4 to about pH 5, or is at about pH 5.0.+-.1.0.
In other embodiments, the compositions contain an
isotonicity-adjusting agent to render the solution or suspension
isotonic and more compatible for injection. Non-limiting examples
of isotonicity agents include NaCl, dextrose, glucose, glycerin,
sorbitol, xylitol, and ethanol. In certain embodiments, the
isotonicity agent is NaCl. In certain embodiments, NaCl is in a
concentration of about 160.+-.20 mM, or about 140 mM.+-.20 mM, or
about 120.+-.20 mM, or about 100 mM.+-.20 mM, or about 80 mM.+-.20
mM, or about 60 mM.+-.20 mM.
In yet other embodiments, the compositions comprise a preservative.
Preservatives include, but are not limited to, m-cresol and benzyl
alcohol. In certain embodiments, the preservative is in a
concentration of about 0.4%.+-.0.2%, or about 1%.+-.0.5%, or about
1.5%.+-.0.5%, or about 2.0%.+-.0.5%.
In still other embodiments, the compositions contain an
anti-adsorbent (e.g., to mitigate adsorption of a CNP variant to
glass or plastic). Anti-adsorbents include without limitation
benzyl alcohol, Polysorbate 20, and Polysorbate 80. In certain
embodiments, the anti-adsorbent is in a concentration from about
0.001% to about 0.5%, or from about 0.01% to about 0.5%, or from
about 0.1% to about 1%, or from about 0.5% to about 1%, or from
about 0.5% to about 1.5%, or from about 0.5% to about 2%, or from
about 1% to about 2%.
In additional embodiments, the compositions comprise a stabilizer.
Non-limiting examples of stabilizers include glycerin, glycerol,
thioglycerol, methionine, and ascorbic acid and salts thereof. In
some embodiments, when the stabilizer is thioglycerol or ascorbic
acid or a salt thereof, the stabilizer is in a concentration from
about 0.1% to about 1%. In other embodiments, when the stabilizer
is methionine, the stabilizer is in a concentration from about
0.01% to about 0.5%, or from about 0.01% to about 0.2%. In still
other embodiments, when the stabilizer is glycerin, the stabilizer
is in a concentration from about 5% to about 100% (neat).
In further embodiments, the compositions contain an anti-oxidant.
Exemplary anti-oxidants include without limitation methionine and
ascorbic acid. In certain embodiments, the molar ratio of
antioxidant to CNP variant is from about 0.1:1 to about 15:1, or
from about 1:1 to about 15:1, or from about 0.5:1 to about 10:1, or
from about 1:1 to about 10:1 or from about 3:1 to about 10:1.
Pharmaceutically acceptable salts can be used in the compositions,
including without limitation mineral acid salts (e.g.,
hydrochloride, hydrobromide, phosphate, sulfate), salts of organic
acids (e.g., acetate, propionate, malonate, benzoate, mesylate,
tosylate), and salts of amines (e.g., isopropylamine,
trimethylamine, dicyclohexylamine, diethanolamine). A thorough
discussion of pharmaceutically acceptable salts is found in
Remington's Pharmaceutical Sciences, 18.sup.th Edition, Mack
Publishing Company, (Easton, Pa. (1990)).
The pharmaceutical compositions can be administered in various
forms, such as tablets, capsules, granules, powders, solutions,
suspensions, emulsions, ointments, and transdermal patches. The
dosage forms of the compositions can be tailored to the desired
mode of administration of the compositions. For oral
administration, the compositions can take the form of, e.g., a
tablet or capsule (including softgel capsule), or can be, e.g., an
aqueous or nonaqueous solution, suspension or syrup. Tablets and
capsules for oral administration can include one or more commonly
used excipieints, diluents and carriers, such as mannitol, lactose,
glucose, sucrose, starch, corn starch, sodium saccharin, talc,
cellulose, magnesium carbonate, and lubricating agents (e.g.,
magnesium stearate, sodium stearyl fumarate). If desired,
flavoring, coloring and/or sweetening agents can be added to the
solid and liquid formulations. Other optional ingredients for oral
formulations include without limitation preservatives, suspending
agents, and thickening agents. Oral formulations can also have an
enteric coating to protect the CNP variant from the acidic
environment of the stomach. Methods of preparing solid and liquid
dosage forms are known, or will be apparent, to those skilled in
this art (see, e.g., Remington's Pharmaceutical Sciences,
referenced above).
Formulations for parenteral administration can be prepared, e.g.,
as liquid solutions or suspensions, as solid forms suitable for
solubilization or suspension in a liquid medium prior to injection,
or as emulsions. For example, sterile injectable solutions and
suspensions can be formulated according to techniques known in the
art using suitable diluents, carriers, solvents (e.g., buffered
aqueous solution, Ringer's solution, isotonic sodium chloride
solution), dispersing agents, wetting agents, emulsifying agents,
suspending agents, and the like. In addition, sterile fixed oils,
fatty esters, polyols and/or other inactive ingredients can be
used. As further examples, formulations for parenteral
administration include aqueous sterile injectable solutions, which
can contain anti-oxidants, buffers, bacteriostats, and solutes that
render the formulation isotonic with the blood of the intended
recipient; and aqueous and nonaqueous sterile suspensions, which
can contain suspending agents and thickening agents.
Compositions comprising a CNP variant can also be lyophilized
formulations. In certain embodiments, the lyophilized formulations
comprise a buffer and bulking agent, and optionally an antioxidant.
Exemplary buffers include without limitation acetate buffers and
citrate buffers. Exemplary bulking agents include without
limitation mannitol, sucrose, dexran, lactose, trehalose, and
povidone (PVP K24). In certain embodiments, mannitol is in an
amount from about 3% to about 10%, or from about 4% to about 8%, or
from about 4% to about 6%. In certain embodiments, sucrose is in an
amount from about 6% to about 20%, or from about 6% to about 15%,
or from about 8% to about 12%. Exemplary anti-oxidants include, but
are not limited to, methionine and ascorbic acid.
The disclosure also provides kits containing, e.g., bottles, vials,
ampoules, tubes, cartridges and/or syringes that comprise a liquid
(e.g., sterile injectable) formulation or a solid (e.g.,
lyophilized) formulation. The kits can also contain
pharmaceutically acceptable vehicles or carriers (e.g., solvents,
solutions and/or buffers) for reconstituting a solid (e.g.,
lyophilized) formulation into a solution or suspension for
administration (e.g., by injection), including without limitation
reconstituting a lyophilized formulation in a syringe for injection
or for diluting concentrate to a lower concentration. Furthermore,
extemporaneous injection solutions and suspensions can be prepared
from, e.g., sterile powder, granules, or tablets comprising a
CNP-containing composition. The kits can also include dispensing
devices, such as aerosol or injection dispensing devices, pen
injectors, autoinjectors, needleless injectors, syringes, and/or
needles.
As a non-limiting example, a kit can include syringes having a
single chamber or dual chambers. For single-chamber syringes, the
single chamber can contain a liquid CNP formulation ready for
injection, or a solid (e.g., lyophilized) CNP formulation or a
liquid formulation of a CNP variant in a relatively small amount of
a suitable solvent system (e.g., glycerin) that can be
reconstituted into a solution or suspension for injection. For
dual-chamber syringes, one chamber can contain a pharmaceutically
acceptable vehicle or carrier (e.g., solvent system, solution or
buffer), and the other chamber can contain a solid (e.g.,
lyophilized) CNP formulation or a liquid formulation of a CNP
variant in a relatively small amount of a suitable solvent system
(e.g., glycerin) which can be reconstituted into a solution or
suspension, using the vehicle or carrier from the first chamber,
for injection.
As a further example, a kit can include one or more pen injector or
autoinjector devices, and dual-chamber cartridges. One chamber of a
cartridge can contain a pharmaceutically acceptable vehicle or
carrier (e.g., solvent system, solution or buffer), and the other
chamber can contain a solid (e.g., lyophilized) CNP formulation or
a liquid formulation of a CNP variant in a relatively small amount
of a suitable solvent system (e.g., glycerin) which can be
reconstituted into a solution or suspension, using the vehicle or
carrier from the first chamber, for injection. A cartridge can
comprise an amount of the CNP variant that is sufficient for dosing
over a desired time period (e.g., 1 day, 2 days, 3 days, 1 week, 2
weeks, 3 weeks, 4 weeks, etc.). The pen injector or autoinjector
can be adjusted to administer a desired amount of the CNP
formulation from a cartridge.
In addition, pharmaceutical compositions comprising a CNP variant
can be formulated as a slow release, controlled release or
sustained release system for maintaining a relatively constant
level of dosage over a desired time period, such as 1 week, 2
weeks, 3 weeks, 1 month, 2 months, or 3 months. Slow release,
controlled release and sustained release formulations can be
prepared using, e.g., biodegradable polymeric systems {which can
comprise, e.g., hydrophilic polymers [e.g., polylactide,
polyglycolide, poly(lactide-glycolide)]}, and can take the form of,
e.g., microparticles, microspheres or liposomes, as is known in the
art.
Dosages and Frequency of Dosing
As used herein, the term "therapeutically effective amount" of an
active agent (e.g., a CNP variant) refers to an amount that
provides therapeutic benefit to a patient. The amount may vary from
one individual to another and may depend upon a number of factors,
including the overall physical condition of the patient. A
therapeutically effective amount of a CNP variant can be readily
ascertained by one skilled in the art, using publicly available
materials and procedures. For example, the amount of a CNP variant
used for therapy should give an acceptable rate of growth based on
growth charts for children ages 0-17 years with achondroplasia (214
females and 189 males), which list height for age, head
circumference, and segmental growth (Horton W A et al., Standard
growth curves for achondroplasia, J. Pediatr., 93: 435-8 (1978)).
CDC charts can be used to assess weight for age and weight for
height or BMI for age. Secondary outcomes with courses that are
more chronic in nature can also be measured.
Having a longer serum half-life than wild-type CNP22, the CNP
variants can potentially be administered less frequently than
CNP22. The dosing frequency for a particular individual may vary
depending upon various factors, including the disorder being
treated and the condition and response of the individual to the
therapy. In certain embodiments, a pharmaceutical composition
containing a CNP variant is administered to a subject about one
time per day, one time per two days, one time per three days, or
one time per week. In one embodiment, for treatment of bone-related
disorders (e.g., skeletal dysplasias, including achondroplasia), a
daily or weekly dose of a CNP variant is administered to patients
until and/or through adulthood.
The CNP variants described herein can be administered to patients
at therapeutically effective doses to treat, ameliorate or prevent
bone-related disorders (e.g., skeletal dysplasias, including
achondroplasia) and conditions (e.g., vascular smooth muscle
disorders) in which CNP can provide a vasoprotective effect. The
safety and therapeutic efficacy of the CNP variants can be
determined by standard pharmacological procedures in cell cultures
or experimental animals, such as, for example, by determining the
LD.sub.50 (the dose lethal to 50% of the population) and the
ED.sub.50 (the dose therapeutically effective in 50% of the
population). The dose ratio between toxic and therapeutic effects
is the therapeutic index and it can be expressed as the ratio
LD.sub.50/ED.sub.50. Active agents exhibiting a large therapeutic
index are normally preferred.
Data obtained from cell culture assays and animal studies can be
used to formulate a range of dosage for use in humans. The dosage
normally lies within a range of circulating concentrations that
include the ED.sub.50, with little or minimal toxicity. The dosage
can vary within this range depending upon the dosage form employed
and the route of administration utilized. The therapeutically
effective dose can be determined from cell culture assays and
animal studies.
In certain embodiments, the CNP variants described herein are
administered at a dose in the range from about 5 or 10 nmol/kg to
about 300 nmol/kg, or from about 20 nmol/kg to about 200 nmol/kg.
In some embodiments, the CNP variants are administered at a dose of
about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75,
80, 85, 90, 95, 100, 110, 120, 125, 130, 140, 150, 160, 170, 175,
180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300,
350, 400, 450, 500, 750, 1000, 1250, 1500, 1750 or 2000 nmol/kg or
other dose deemed appropriate by the treating physician. In other
embodiments, the CNP variants are administered at a dose of about
5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140,
150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550,
600, 650, 700, 750, 800, 850, 900, 950 or 1000 ug/kg, or about 1,
1.25, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5,
9, 9.5 or 10 mg/kg, or other dose deemed appropriate by the
treating physician. The doses of CNP variants described herein can
be administered according to the dosing frequency/frequency of
administration described herein, including without limitation
daily, 2 or 3 times per week, weekly, every 2 weeks, every 3 weeks,
monthly, etc.
The frequency of dosing/administration of a CNP variant for a
particular subject may vary depending upon various factors,
including the disorder being treated and the condition and response
of the subject to the therapy. The CNP variant can be administered
in a single dose or in multiple doses per dosing. In certain
embodiments, the CNP variant is administered, in a single dose or
in multiple doses, daily, every other day, every 3 days, 2 times
per week, 3 times per week, weekly, bi-weekly, every 3 weeks,
monthly, every 6 weeks, every 2 months, every 3 months, or as
deemed appropriate by the treating physician.
In some embodiments, a CNP variant is administered so as to allow
for periods of growth (e.g., chondrogenesis), followed by a
recovery period (e.g., osteogenesis). For example, the CNP variant
may be administered intravenously, subcutaneously or by another
mode daily or multiple times per week for a period of time,
followed by a period of no treatment, then the cycle is repeated.
In some embodiments, the initial period of treatment (e.g.,
administration of the CNP variant daily or multiple times per week)
is for 3 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks,
7 weeks, 8 weeks, 9 weeks, 10 weeks, 11 weeks or 12 weeks. In a
related embodiment, the period of no treatment lasts for 3 days, 1
week, 2 weeks, 3 weeks or 4 weeks. In certain embodiments, the
dosing regimen of the CNP variant is daily for 3 days followed by 3
days off; or daily or multiple times per week for 1 week followed
by 3 days or 1 week off; or daily or multiple times per week for 2
weeks followed by 1 or 2 weeks off; or daily or multiple times per
week for 3 weeks followed by 1, 2 or 3 weeks off; or daily or
multiple times per week for 4, 5, 6, 7, 8, 9, 10, 11 or 12 weeks
followed by 1, 2, 3 or 4 weeks off.
Modes of Administration
The CNP variants, or pharmaceutical compositions comprising them,
can be administered to subjects in various ways such as, e.g., by
injection subcutaneously, intravenously, intra-arterially,
intraperitoneally, intramuscularly, intradermally, or
intrathecally. In an embodiment, the CNP variants are administered
by a single subcutaneous, intravenous, intra-arterial,
intraperitoneal, intramuscular, intradermal or intrathecal
injection once a day.
The CNP variants can also be administered by direct injection at or
near the site of disease. Further, the CNP variants can be
administered by implantation of a depot at the target site of
action (e.g., an abnormal or dysplasic bone). Alternatively, the
CNP variants can be administered sublingually under the tongue
(e.g., sublingual tablet) or by inhalation into the lungs (e.g.,
inhaler or aerosol spray), by delivery into the nasal cavity (e.g.,
intranasal spray), by delivery into the eye (e.g., eye drop), or by
transdermal delivery (e.g., by means of a patch on the skin). The
CNP variants may also be administered orally in the form of
microspheres, microcapsules, liposomes (uncharged or charged (e.g.,
cationic)), polymeric microparticles (e.g., polyamides,
polylactide, polyglycolide, poly(lactide-glycolide)),
microemulsions, and the like.
A further method of administration is by osmotic pump (e.g., an
Alzet pump) or mini-pump (e.g., an Alzet mini-osmotic pump), which
allows for controlled, continuous and/or slow-release delivery of
the CNP variant or pharmaceutical composition over a pre-determined
period. The osmotic pump or mini-pump can be implanted
subcutaneously, or near the target site (e.g., the long bones of
limbs, the epiphyses, etc).
As explained above, the CNP variants can be used to treat
conditions or diseases caused by the abnormal growth of vascular
smooth muscle cells, including but not limited to restenosis and
arteriosclerosis. For local delivery of a CNP variant to the
diseased bodily vessel (e.g., blood vessel), the CNP variant can be
delivered by means of a medical device (e.g., a stent) implanted at
the diseased site. In one embodiment, the CNP variant is
impregnated in a polymeric matrix or polymeric coating disposed
over a stent. In another embodiment, the CNP variant is contained
in reservoirs or channels formed in the body of a stent and covered
by a porous polymeric membrane or layer through which the CNP
variant can diffuse. The polymeric matrix, coating, membrane or
layer can comprise at least one biodegradable (e.g., hydrophilic)
polymer, as is known in the art. In a further embodiment, the CNP
variant can be contained in micropores in the body of a stent. The
CNP variant can be delivered from a stent by burst release, pulse
release, controlled release or sustained release, or a combination
thereof. For example, the stent can locally deliver the CNP variant
to the diseased site in a burst release followed by a sustained
release. Sustained release can be over a period up to about 2
weeks, 1 month, 2 months, 3 months, 6 months or 1 year.
It will be apparent to one skilled in the art that the CNP variants
or compositions thereof can also be administered by other modes.
Determination of the most effective mode of administration of the
CNP variants or compositions thereof is within the skill of the
skilled artisan.
The CNP variants can be administered as pharmaceutical formulations
suitable for, e.g., oral (including buccal and sub-lingual),
rectal, nasal, topical, pulmonary, vaginal or parenteral (including
intramuscular, intraarterial, intrathecal, subcutaneous and
intravenous) administration, or in a form suitable for
administration by inhalation or insufflation. Depending on the
intended mode of administration, the pharmaceutical formulations
can be in the form of solid, semi-solid or liquid dosage forms,
such as tablets, suppositories, pills, capsules, powders, liquids,
suspensions, emulsions, creams, ointments, lotions, and the like.
The formulations can be provided in unit dosage form suitable for
single administration of a precise dosage. The formulations
comprise an effective amount of a CNP variant, and one or more
pharmaceutically acceptable excipients, carriers and/or diluents,
and optionally one or more other biologically active agents.
Combination Therapy
In one embodiment, a CNP variant can be used in combination with
one or more other active agents useful for treating, ameliorating
or preventing CNP-responsive conditions or disorders such as, e.g.,
bone-related disorders (e.g., skeletal dysplasias) and vascular
smooth muscle disorders. The other active agent(s) can enhance the
effects of the CNP variant and/or exert other pharmacological
effects in addition to those of the CNP variant. Non-limiting
examples of active agents that can be used in combination with the
CNP variants described herein are other natriuretic peptides (e.g.,
BNP) and inhibitors (e.g., antagonists) of peptidases and proteases
(e.g., NEP and furin), NPR-C and tyrosine kinases (e.g., FGFR-3).
By preventing NEP cleavage of the CNP variant, an NEP inhibitor can
prolong the half-life of the variant. Examples of NEP inhibitors
include, without limitation, thiorphan and candoxatril. Co-use of
an NPR-C inhibitor can also prolong the half-life of the CNP
variant via inhibition of the variant's clearance by NPR-C. A
non-limiting example of an NPR-C inhibitor is the fragment
FGIPMDRIGRNPR (SEQ ID NO: 82), which would be released at the
target site (e.g., bone growth plate) upon proteolytic cleavage of
the FGIPMDRIGRNPR-CNP22 chimera (Analog CZ) (SEQ ID NO: 82) or
similar chimeras comprising variants of CNP22 (e.g., those
containing amino acid substitution(s), addition(s), and/or
deletion(s) relative to CNP22). Co-use of a tyrosine kinase
inhibitor can accentuate the effects of a CNP therapy by inhibiting
the tyrosine kinase receptor FGFR-3, a negative regulator of
chondrocyte and bone growth. Non-limiting examples of tyrosine
kinase inhibitors include those disclosed in U.S. Pat. Nos.
6,329,375 and 6,344,459.
To achieve the appropriate therapeutic outcome in the combination
therapies, one would generally administer to the subject the CNP
composition and other therapeutic(s) in a combined amount effective
to produce the desired therapeutic outcome (e.g., restored bone
growth). This process may involve administering the CNP composition
and other therapeutic agent(s) at the same time. Simultaneous
administration can be achieved by administering a single
composition or pharmacological protein formulation that includes
both the CNP variant and other therapeutic agent(s). Alternatively,
the other therapeutic agent(s) can be taken separately at about the
same time as a pharmacological formulation (e.g., tablet, injection
or drink) of the CNP variant. The CNP variant can also be
formulated into a foodstuff such as brownies, pancakes, or cake,
suitable for ingestion.
In other alternatives, administration of the CNP variant can
precede or follow administration of the other therapeutic agent(s)
by intervals ranging from minutes to hours. In embodiments where
the other therapeutic agent(s) and the CNP composition are
administered separately, one would generally ensure that the CNP
variant and the other therapeutic agent(s) are administered within
an appropriate time of one another so that both the CNP variant and
the other therapeutic agent(s) can exert, synergistically or
additively, a beneficial effect on the patient. For example, one
can administer the CNP composition within about 0.5-6 hours (before
or after) of the other therapeutic agent(s). In one embodiment, the
CNP composition is administered within about 1 hour (before or
after) of the other therapeutic agent(s).
Identifying and Monitoring Patient Populations
Protocols can be established to identify subjects suitable for CNP
therapy and to determine whether a given patient is responsive to
CNP therapy. For example, for treatment of bone-related disorders,
indicators of growth can be measured, such as long bone growth
measurements in utero and neonatal and measurements of bone growth
biomarkers such as CNP, cGMP, Collagen II, osteocalcin, and
Proliferating Cell Nuclear Antigen (PCNA).
One CNP signaling marker is cGMP (guanosine 3',5' cyclic
monophosphate). The level of this intracellular signaling molecule
increases after CNP binds to and activates its cognate receptor
NPR-B. Elevated levels of cGMP can be measured from cell culture
extracts (in vitro) after CNP exposure, conditioned media from bone
ex-plant studies (ex vivo) after CNP exposure, and in the plasma
(in vivo) within minutes of CNP administration subcutaneously,
intravenously, or via other routes of administration known in the
art.
Cartilage and bone-specific analytes (or cartilage- and
bone-associated markers) can also be measured to assess CNP
efficacy. For example, fragments of cleaved collagen type II are a
cartilage-specific marker for cartilage turnover. Type II collagen
is the major organic constituent of cartilage and fragments of type
II collagen (cleaved collagen) are released into circulation, and
subsequently secreted into the urine, following cartilage turnover.
Cartilage turnover preceeds new bone formation.
A bone-specific biomarker for bone formation which can be measured
is N-terminal propeptides of type I procollagen (PINP). The
synthesis of type I collagen is an important step in bone
formation, as type I collagen is the major organic component in
bone matrix. During collagen synthesis, propeptides are released
from the procollagen molecule and can be detected in serum. In
addition, fragments of collagen type I can be measured as a marker
for bone resorption.
Other potential biomarkers for cartilage and bone formation and
growth include aggrecan chondroitin sulfate (cartilage-specific
marker for cartilage turnover), propeptides of type TT collagen
(cartilage-specific marker for cartilage formation), alkaline
phosphatase (bone-specific) and osteocalcin (bone-specific marker
for bone formation). Cartilage- and bone-associated biomarkers can
be measured, e.g., in serum from efficacy/pharmacodynamic in vivo
studies and from the conditioned media of ex vivo studies, using
commercially available kits.
In one embodiment, the level of at least one bone- or
cartilage-associated biomarker is assayed or measured in a subject
that has been administered a CNP variant in order to monitor the
effects of the CNP variant on bone and cartilage formation and
growth in vivo. For example, an increase in the level of at least
one bone- or cartilage-associated biomarker may indicate that
administration of a CNP variant has a positive effect on bone
growth and is a useful treatment for skeletal dysplasias and other
bone- or cartilage-related diseases or disorders associated with
decreased CNP activity. Exemplary bone- or cartilage-associated
biomarkers include, but are not limited to, CNP (e.g, endogenous
levels of CNP), cGMP, propeptides of collagen type II and fragments
thereof, collagen type II and fragments thereof, osteocalcin,
proliferating cell nuclear antigen (PCNA), propeptides of type I
procollagen (PINP) and fragments thereof, collagen type I and
fragments thereof, aggrecan chondroitin sulfate, and alkaline
phosphatase.
In an embodiment, biomarkers are measured by obtaining a biological
sample from a subject who will be administered, is being
administered or has been administered a CNP variant. Biomarkers can
be measured using techniques known in the art, including, but not
limited to, Western Blot, enzyme linked immunosorbant assay
(ELISA), and enzymatic activity assay. The biological sample can be
blood, serum, urine, or other biological fluids.
Additional aspects and details of the disclosure will be apparent
from the following examples, which are intended to be illustrative
rather than limiting.
F. Examples
Example 1
Synthesis of CNP Variants
CNP variants were prepared using the methods described herein.
Substitutions with natural or unnatural amino acids or
peptidomimetics were made, as indicated in Tables 1-3 (shown in
Example 3), at the respective amino acid residues in the wild-type
sequence of CNP22. In certain variants, additional amino acids were
added to the N-terminal and/or C-terminal ends of the whole or a
portion of the wild-type CNP22 sequence (see Table 3).
Also prepared were CNP variants in which a PEG (or PEO) moiety was
conjugated to the N-terminus of CNP22 or variants thereof (see
Table 4, shown in Example 3). PEGylation reagents can be obtained
from the commercial sources shown in Table 5.
TABLE-US-00028 TABLE 5 MW Vendor Product Name Name (Da) PEGylation
Reagent NOF Sunbright ME-200CS mPEG20K 20,000 CH3 (CH2CH2O)450-
(CH2)5COO--NHS NOF Sunbright ME 050CS mPEG5K 5,000 CH3(CH2CH2O)110-
(CH2)5COO--NHS Pierce (Methyl-PEG12)3-PEG4-NHS (mPEG12)3- 2,400
[CH3(CH2CH2O)12]3- Ester PEG4 (CH2CH2O)4-NHCO(CH2)3- COO--NHS NOF
Sunbright ME-020HS mPEG2K 2,000 CH3(CH2CH2O)45- (CH2)5COO--NHS NOF
Sunbright ME-020CS mPEG2K 2,000 CH3(CH2CH2O)45- (CH2)5COO--NHS NOF
Sunbright ME-010HS mPEG1K 1,000 CH3(CH2CH2O)23- CO(CH2)2COO--NHS
Pierce Methyl PEG24-NHS Ester MS(PEG)24 1,200 CH3(CH2CH2O)24-
(CH2)2COO--NHS Pierce EZ-Link NHS-PEG12-Biotin PEO12-Biotin 940
Biotin-(CH2CH2O)12- (CH2)2COO--NHS Pierce Methyl PEG12-NHS Ester
MS(PEG)12 690 CH3(CH2CH2O)12- (CH2)2COO--NHS Pierce EZ-Link
NHS-PEG4-Biotin PEO4-biotin 590 Biotin-(CH2CH2O)4- (CH2)2COO--NHS
Pierce Mono(lactosylamido) LSS 590 mono(succinimidyl)suberate
Pierce EZ-link NHS-LC-LC-Biotin LC-LC-Biotin 570 Pierce EZ-link
NHS-LC-Biotin LC-Biotin 450 (LC = long chain) Pierce EZ-link
NHS-Biotin Biotin 340
The PEG (also called PEO) polymers purchased from Pierce
Biotechnology (Rockford, Ill.) are monodispersed--i.e., they
contain a single discrete polymer of a particular molecular weight.
By contrast, the PEG polymers purchased from NOF (Nippon Oil and
Fat) are polydispersed--i.e., they contain a mixture of polymers
having a distribution of molecular weights.
To PEGylate CNP22 or variants thereof, reaction and purification
conditions are optimized for each PEG-CNP conjugate. According to a
general PEGylation procedure, reaction mixtures contain about 1 mM
CNP22 or a variant thereof, and about 1 to 5 mM NHS-activated PEG
in potassium phosphate buffer, pH between about 5.0 and 6.5. To
mono-PEGylate selectively at the peptide N-terminus and minimize
PEGylation at an internal site (e.g., Lys4 of CNP22), the
PEGylation reaction can be conducted under more acidic conditions
(e.g., at a pH between about 5.5 and 6.5) to protonate selectively
and hence deactivate the more basic primary amino group on the
lysine side chain. After about 1 to 3 hours of incubation at room
temperature, the PEGylation reaction is quenched by addition of
aqueous glycine buffer. Reaction products are then separated by
reverse-phase HPLC, optimized for each PEG-CNP conjugate.
Fractionation samples are speedvacced to dryness, and
reconstituted/formulated in 1 mM HCl. Identification and purity of
each PEG-CNP product are determined by liquid chromatography-mass
spectrometry (LC/MS).
Example 2A
Recombinant Production of CNP Variants
CNP variants can be produced using recombinant technology. In
certain embodiments, the CNP variants are produced as fusion
proteins comprising a cleavable peptide, carrier protein or tag.
Exemplary methods for recombinantly producing CNP fusion proteins
are disclosed below.
Materials and Methods
Cloning of CNP Fusion Proteins into Expression Vectors
CNP DNA fragments were amplified using polymerase chain reaction
(PCR) and the amplified PCR fragments were digested with Nde I and
BamHI and cloned into pET21a vector (Novagen, Gibbstown, N.J.). CNP
fusion protein DNA was synthesized by DNA2.0 and cloned into
different expression vectors (Table 6).
TABLE-US-00029 TABLE 6 Chemical Final Expression Construct Vector
Product cleavage product E. coli strain pJexpress- pJexpress401
TAF-CNP Formic acid Pro-CNP BL21; TAF-CNP inclusion bodies
(Asp-Pro) BL21(DE3) pJexpress- pJexpress404 KSI-CNP(M/N) CNBr (Met-
CNP(M/N) BL21 KSI- inclusion bodies X) CNP(M/N) pET-31b- pET-31b
KSI-CNP inclusion Formic acid Pro-CNP BL21(DE3) KSI-CNP bodies
(Asp-Pro) pET-32a- pET-32a Trx-CNP fusion Formic acid Pro-CNP
BL21(DE3) Trx-CNP protein (soluble) (Asp-Pro) pMAL- pMAL-c2X
MBP-CNP fusion Formic acid Pro-CNP BL21(DE3) CNP protein (soluble)
(Asp-Pro) CNP: GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC .left
brkt-bot.Gly-CNP37 (SEQ ID NO: 75); TAF: human transcription factor
TAF12; KSI: ketosteroid isomerase; MBP: maltose-binding protein;
Trx: thioredoxin
Expression of CNP Fusion Proteins in E. coli
CNP fusion protein expression plasmids were transformed into E.
coli BL21 or BL21(DE3). Transformed cells were plated on LB plates
containing 100 ug/ml carbeniciline or 50 ug/ml kanamycin and
incubated overnight at 37.degree. C. One single colony was picked
and cultured in 4 ml LB medium containing 100 ug/ml of
carbeniciline or 50 ug/ml kanamycin at 37.degree. C. with shaking.
When an OD.sub.600 of bacterial culture reached 0.6, 1 mM isopropyl
.beta.-D-1-thiogalactopyranoside (IPTG) was added to the cell media
and the media was incubated at 37.degree. C. for 3 hours with
shaking. For cell harvest, bacterial cells were centrifuged at 4000
rpm for 10 minutes and the cell pellets were stored at -80.degree.
C. Cell pellets were lysed with B-PER II Bacterial Extraction
Reagent (PIERCE, 0.4 ml per 4 ml of bacterial culture) and
Benzonase Nuclease (Novagen, 0.025 U/ml) at room temperature for 10
minutes. Bacterial crude extract was reserved and centrifuged to
obtain supernatant. Supernatant and crude extract were assayed for
CNP fusion protein expression and solubility by SDS-PAGE and
Western Blot.
Detection of CNP Fusion Protein Expression with SDS-PAGE and
Western Blot
Ten uL of cell lysates or soluble supernatants was run on sodium
dodecyl sulfate-polyacrylimide gel electrophoresis (SDS-PAGE)
(Invitrogen, Carlsbad, Calif., NuPAGE 4-12% Bis-tris Gel, MES SDS
buffer). The gel was stained using 20 ml Imperial Protein Stain
(Thermo Fisher, Rockford, Ill.) at room temperature for 1 hour and
de-stained with water. For Western blot, the protein was
transferred to membrane with Gel blot (Invitrogen). The membrane
was blocked in TBS buffer with 5% milk at room temperature for 1
hour. Rabbit anti-CNP22 antibody (1:2500 dilution) (Bachem,
Torrance, Calif.) was added to the membrane, which was then
incubated at room temperature with shaking for 2 hours, and then
the membrane was washed 3 times with TBS buffer.
Alkaline phosphate (AP) conjugated anti-rabbit IgG (1:5000
dilution) was added to the membrane, which was then incubated at
room temperature with shaking for 1 hour, and then the membrane was
washed 3 times with TBS buffer. Ten ml WESTERN BLUE.RTM.Stabilized
Substrate (Promega, Madison, Wis.) was added to the membrane, which
was then incubated at room temperature with shaking for 1 to 5 min,
and then the membrane was washed with TBS buffer to remove excess
stain.
Expression of TAF-CNP Fusion Protein in E. coli BL21
Cells (E. coli strain BL21) expressing TAF-CNP fusion protein were
obtained from glycerol stock stored at -80.degree. C. and were
grown in 4 ml LB medium containing 50 ug/ml of kanamycin at
37.degree. C. overnight with shaking (250 rpm). Four ml of
overnight grown cell culture was transferred to 200 ml LB medium
containing 50 ug/ml of kanamycin and was grown at 37.degree. C.
with shaking (250 rpm). When the OD.sub.600 reached 0.6, IPTG was
then added to a final concentration of 1 mM to induce protein
expression at 37.degree. C. with shaking (250 rpm) for 3 hours.
Cells were then spun down at 3000 rpm for 10 minutes and the
resulting cell pellet was frozen at -80.degree. C.
Purification of TAF-CNP Inclusion Bodies and Formic Acid
Cleavage
The cell pellet (from 200 ml culture) was resuspended in 25 ml of
B-PER II buffer (PIERCE), the pellet was sonicated for 10 minutes
(50%, 1 second, pause 2 seconds) on ice, centrifuged at 12000 rpm
for 20 minutes at 4.degree. C., and then the pellet was resuspended
in 25 ml 20.times. diluted B-PER II buffer. This was repeated until
the supernatant became clear (3-5 times). One mL of resuspended
TAF-CNP inclusion bodies was transferred to a 1.5 ml tube and
centrifuged at 14000 rpm for 15 min. The supernatant was discarded
and the pellet was dissolved with 10 ul of 88% formic acid, and
then 490 ul of Millipore filtered water was added immediately. The
pellet was mixed well by vortex and incubated at 55.degree. C. for
20 to 24 hours (70.degree. C./6 hours are alternative conditions).
The products of the formic acid cleavage were assayed by SDS-PAGE
and LC/MS (C4RP).
LC/MS Sample Preparation
Inclusion bodies were isolated from about 8 mL of culture (about
1.5 OD) and the pellet was solubilized in 10 uL nt formate.
Resolubilized pellet was immediately diluted to 2% or 10% final
formate concentration (0.5 mL) and incubated at 55.degree. C. for
21 hours (pH 2) (cloudiness was more evident in the 2% formate
sample the next day). Both samples were centrifuged at 15000 rpm
for 2 minutes. Twenty uL of supernatant was injected into an LC/MS
(C4 RP) apparatus.
Results
CNP Fusion Proteins were Expressed in E. coli
All CNP fusion proteins were expressed in E. coli induced with 1 mM
IPTG at 37.degree. C. for 3 hours (FIG. 1). The constructs
pJexpress-TAF-CNP, pJexpress-KSI-CNP(M/N) and pET-31b-KSI-CNP were
expressed as inclusion bodies, while the constructs pET-32a-Trx-CNP
and pMAL-CNP were expressed as soluble fusion proteins. Western
blot using anti-CNP22 antibody confirmed the expression of the CNP
fusion proteins (FIG. 1).
CNP was Produced from TAF-CNP Inclusion Bodies by Formic Acid
Cleavage
TAF-CNP inclusion bodies were partially purified and treated with
2% formic acid at 55.degree. C. for 20 to 24 hours (70.degree. C./6
hours are alternative conditions). The majority of TAF-CNP was
cleaved and one extra band having similar size as Gly-CNP37 peptide
appeared on SDS-PAGE (FIG. 2). The cleaved sample was further
analyzed by LC/MS (C4 RP). The LC/MS results showed that CNP was
released in soluble form from TAF-CNP inclusion bodies after formic
acid cleavage. LC/MS analysis indicated that formic acid cleavage
of the CNP fusion proteins resulted in formation of cyclized
Pro-Gly-CNP37 (MW=4102). Calculation of protein amounts based on
analysis suggested that about 60 ug of formic acid-generated CNP
was produced from 8 mL of very low OD culture. From a small scale
(e.g., about 8 mL) of low OD (e.g., 1.2 OD) cell culture,
approximately 8 ug/ml CNP was produced, while fermentation of a
larger scale (e.g., about 8 L) of higher OD (e.g., 380D) cell
culture can produce approximately 1 mg/ml CNP.
Conclusion
Five expression constructs were generated to express CNP fusion
proteins. Expression of all five constructs produced soluble (Trx
and MBP) or insoluble (TAF and KSI) CNP fusion proteins.
Approximately 1 mg/ml soluble CNP can be produced from TAF-CNP
inclusion bodies by a simple formic acid cleavage procedure.
Example 2B
Production of Additional CNP Variants in E. coli
Recombinant production of CNP variants was carried out as described
in Example 2A. In this example, additional CNP constructs were
generated with a QuikChange II XL site-directed mutagenesis kit
(Stratagene) or were synthesized by DNA2.0. Additional CNP
constructs and expression vectors are listed in Table 7.
TABLE-US-00030 TABLE 7 Chemical Final Expression Construct Vector
Product cleavage product E. coli strain pJexpress- pJexpress401
TAF(C/A)-Pro- Formic acid Pro- BL21(DE3) TAF(C/A)-Pro- CNP38
inclusion (Asp-Pro) CNP38 CNP38 bodies pJexpress- pJexpress401
TAF(4D/4E)-Pro- Formic acid Pro- BL21(DE3) TAF(4D/4E)- CNP38
inclusion (Asp-Pro) CNP38 Pro-CNP38 bodies pJexpress-TAF
pJexpress401 TAF(C/A&4D/4E)- Formic acid Pro- BL21(DE3)
(C/A&4D/4E)- Pro-CNP38 inclusion (Asp-Pro) CNP38 Pro-CNP38
bodies pJexpress-TAF pJexpress401 TAF(C/A&10D/10E)- Formic acid
Pro- BL21(DE3) (C/A&10D/10E)- Pro-CNP38 inclusion (Asp-Pro)
CNP38 Pro-CNP38 bodies pJexpress-TAF- pJexpress401 TAF-NL-(C/A
& Formic acid Pro- BL21(DE3) NL-(C/A & 6D/6E)-Pro-CNP38
(Asp-Pro) CNP38 6D/6E)-Pro- inclusion bodies CNP38 pJexpress-BMP-
pJexpress401 BMP-Pro-CNP38 Formic acid Pro- BL21; Pro-CNP38
inclusion bodies (Asp-Pro) CNP38 BL21(DE3) pJexpress-TAF-
pJexpress401 TAF-Pro-CNP37 Formic acid Pro- BL21; Pro-CNP37
inclusion bodies (Asp-Pro) CNP37 BL21(DE3) pJexpress-BMP-
pJexpress401 BMP-Pro-CNP37 Formic acid Pro- BL21; Pro-CNP37
inclusion bodies (Asp-Pro) CNP37 BL21(DE3) pJexpress-TAF
pJexpress401 TAF-Pro-HSA-CNP Formic acid Pro- BL21 Pro-HSA-CNP
inclusion bodies (Asp-Pro) HSA-CNP pJexpress-TAF pJexpress401 TAF
inclusion bodies N/A N/A BL21(DE3) pJexpress-BMP pJexpress401 BMP
inclusion bodies N/A N/A BL21(DE3) pJexpress- pJexpress401 TAF(C/A)
inclusion N/A N/A BL21(DE3) TAF(C/A) bodies pJexpress- pJexpress401
TAF(4D/4E) N/A N/A BL21(DE3) TAF(4D/4E) inclusion bodies
pJexpress-TAF pJexpress401 TAF(C/A&4D/4E) N/A N/A BL21(DE3)
(C/A&4D/4E) inclusion bodies pJexpress-TAF pJexpress401
TAF(C/A&10D/10E) N/A N/A BL21(DE3) (C/A&10D/10E) inclusion
bodies pJexpress-TAF- pJexpress401 TAF-NL-(C/A&6D/6E) N/A N/A
BL21(DE3) NL-(C/A & inclusion bodies 6D/6E) pJexpress-TAF-
pJexpress401 TAF-Pro-CNP53 Formic acid Pro- BL21(DE3) Pro-CNP53
inclusion bodies (Asp-Pro) CNP53 pJexpress-TAF- pJexpress401
TAF-CNP34 Formic acid CNP34 BL21(DE3) CNP34 inclusion bodies
(Asp-Pro) CNP38: GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC [G1y-CNP37
(SEQ ID NO: 75)]; Pro-CNP38:
PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC [Pro-G1y-CNP37](SEQ ID NO:
145); CNP37: QEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 60);
HSA-CNP: GHKSEVAHRFKGANKKGLSKGCFGLKLDRIGSMSGLGC [HSA-CNP27 (SEQ ID
NO: 144)]; Pro-CNP53:
PDLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIG-SMSGLGC (SEQ ID NO:
185); CNP34: PNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 163);
TAF-Pro-CNP38: MVLTKKKLQDLVREVCPNEQLDEDVEEMLLQIADDFIESVVTAA-
CQLARHRKSSTLEVKDVQLHLERQWNM-WIMGSSHHHHHHSSGLVPRGSHT-
GDDDDKHMDPGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC (SEQ ID NO: 196);
TAF: human transcription factor TAF12 histone fold domain (HFD) and
linker from pET-15b vector; TAF-NL: TAF12 HFD without a linker;
TAF12 HFD: VLTKKKLQDLVREVCPNEQLDEDVEEMLLQIADDFIESVVTAACQLA-
RHRKSSTLEVKDVQLHLERQWNMWI (SEQ ID NO: 197); pET-15b linker:
MGSSHHHHHHSSGLVPRGSHTGDDDDKHMD (SEQ ID NO: 195); TAF(C/A): cysteine
In TAF is changed to alanine; TAF(D/E): aspartic acid in TAF is
changed to glutamic acid (number indicates which amino acid residue
is changed); TAF(C/A & D/E) : cysteine and aspartic acid in TAF
are changed to alanine and glutamic acid, respectively; BMP: bone
morphogenetic protein 2 with seven C/A (cysteine to alanine)
mutations; KSI: ketosteroid isomerase; MBP: maltose-binding
protein; TRX: thioredoxin
Results
All CNP fusion proteins were expressed in E. coli induced with 1 mM
IPTG at 37.degree. C. for 3 hours. Constructs
pJexpress-BMP-Pro-CNP38, pJexpress-TAF-Pro-CNP37,
pJexpress-BMP-Pro-CNP37, pJexpress-Pro-HSA-CNP, pJexpress-TAF and
pJexpress-BMP were expressed as inclusion bodies. A Western Blot
with anti-CNP antibody was used to confirm the expression (FIG.
3).
Pro-Gly-CNP37 ("Pro-CNP38") was Produced from TAF-Pro-CNP38
Inclusion Bodies by Formic Acid Cleavage
Formic acid is used as a denaturant for inclusion body proteins and
can specifically cleave the peptide bond between Asp and Pro under
optimized conditions. TAF-Pro-CNP inclusion bodies were partially
purified as described above and treated with 50% formic acid at
25.degree. C., 37.degree. C., 42.degree. C. and 55.degree. C. for
24 hours. Most of the TAF-Pro-CNP38 was cleaved and one extra band
with a similar size as the Gly-CNP37 ("CNP38") peptide showed on
SDS-PAGE from the 37.degree. C., 42.degree. C. and 55.degree. C.
cleavages (FIG. 4A). The cleavage reactions at 37.degree. C. and
55.degree. C. were neutralized with 10 M NaOH and centrifuged at
14,000 rpm for 15 minutes. The un-cleaved TAF-Pro-CNP38, TAF and
other inclusion bodies precipitated in the pellets. The
supernatants contained soluble Pro-CNP38 and were further analyzed
by LC/MS. The LC/MS result showed the supernatants contained a
mixture of non-specific cleaved peptides generated by excess acidic
hydrolysis.
When TAF-Pro-CNP inclusion bodies were treated with 2% and 10%
formic acid at 55.degree. C. for 20 hours, the majority of
TAF-Pro-CNP38 was cleaved and one extra band having a similar size
as Gly-CNP37 was observed on SDS-PAGE (FIG. 4B). The cleaved sample
was further analyzed by LC/MS. The LC/MS analysis showed that
correct Pro-CNP38 was released in soluble form from TAF-Pro-CNP38
inclusion bodies after formic acid cleavage. The yields of
Pro-CNP38 from 2% and 10% formic acid cleavages were similar (FIG.
4C).
Formic Acid Cleavage and Neutralization for Pro-Gly-CNP37
("Pro-CNP38") Production and Purification
Formic acid can dissolve and cleave TAF-Pro-CNP38, BMP-Pro-CNP38
and other inclusion bodies. The pH neutralization results in
precipitation of insoluble contaminating proteins/peptides
(un-cleaved TAF-Pro-CNP38 and BMP-Pro-CNP38, TAF, BMP and others).
The soluble Pro-CNP38 stays in the supernatant after
centrifugation. TAF-Pro-CNP38 and BMP-Pro-CNP38 were cleaved in 2%
formic acid at 55.degree. C. or 70.degree. C. for 24 hours. The
cleavage reactions were neutralized with 1:1 ratio of 0.5 M Tris
buffer and centrifuged at 14,000 rpm for 15 minutes. Results showed
that the supernatants contained almost pure peptides and there was
no observed recovery loss of Pro-CNP38 upon neutralization. This is
a simple and efficient step for Pro-CNP38 purification.
Analysis of Varying Conditions for Formic Acid Cleavage of
TAF-Pro-CNP38 Inclusion Bodies for Pro-Gly-CNP37 ("Pro-CNP38")
Production
Formic acid can specifically cleave the peptide bond between Asp
and Pro under optimized conditions. Non-specific cleavage of
peptide bonds between Asp and any other amino acids or even
non-specific cleavage of any peptide bond may occur if the formic
acid cleavage conditions are not optimized. TAF-Pro-CNP38 inclusion
bodies were cleaved with 2% formic acid at 42.degree. C.,
55.degree. C. or 70.degree. C. for 6, 24 or 48 hours. FIG. 5A shows
that TAF-Pro-CNP38 was cleaved completely at 70.degree. C. for 24
hours or at 55.degree. C. for 48 hours. The 70.degree. C. cleavage
could be completed within 17 hours (FIG. 5B). Although the
70.degree. C./24 h cleavage gave the highest yield, the
non-specific cleavage products (for example, the peptide with
molecular weight of 3142 generated from Pro-CNP38 by cleaving
between peptide bond Asp and Arg) increased dramatically (FIG.
5C).
The yield and purity of Pro-CNP38 production were improved when
TAF-Pro-CNP38 inclusion bodies were purified or treated with B-PER
II buffer before formic acid cleavage. Because B-PER II buffer
contains detergent octylthio-glucoside and is relatively expensive,
other commonly used detergents or buffers were tested for
large-scale Pro-CNP38 production. TAF-Pro-CNP38 inclusion bodies
were re-suspended in different detergents (Octylsucrose; Triton
x-100; Tween-20; NP-40; CA-630) or buffers (B-PER II; B-PER II 1/20
dilution; B-PER; B-PER phosphate buffer; 25 mM tris, 150 mM NaCl,
pH 7.9; 25 mM tris, pH 7.5; filtered water; PBS) and incubated at
room temperature (RT) for 24 hours. All detergents were 1% in 25 mM
tris buffer, pH 7.5. After incubation in detergent or buffer,
TAF-Pro-CNP38 inclusion bodies were cleaved by 2% formic acid at
55.degree. C. for 22 hours. Results showed that BPER II continued
to exhibit good yield, and positive results were also obtained with
CA630 and Triton X-100.
Pro-Gly-CNP37 ("Pro-CNP38") Proteolytic Cleavage Products
One unidentified protease, possibly a membrane associated protease,
cleaved the Pro-CNP38 peptide (produced from BL21 strain, MW 4102)
into two peptides during Pro-CNP38 purification, resulting in
peptides PGQEHPNAR (MW 1004) (SEQ ID NO: 198) and
KYKGANKKGLSKGCFGLKLDRIGSMSGLGC (MW 3115) (SEQ ID NO: 199). Not to
be bound by theory, one possible reason why detergents may improve
the yield and purity of Pro-CNP38 is that detergents can remove
most, but possibly not all, of the unidentified protease. High
temperature, basic pH and EDTA were tested to identify if these
agents could inhibit the protease cleavage. TAF-Pro-CNP38 inclusion
bodies were incubated at RT or 120.degree. C. for 2 hrs and
centrifuged at 14000 rpm for 15 min. The pellets were re-suspended
in 2% formic acid and incubated at 55.degree. C. or 70.degree. C.
for 18 hours. A 1:1 ratio of 0.5 M Tris was added to neutralize the
cleavage. The neutralized samples were centrifuged at 14000 rpm for
5 min and the supernatants were left at RT for 6 hrs or 22 hrs with
or without 10 mM EDTA, pH 10. The proteolytic cleavage was assayed
by LC/MS (Table 8).
TABLE-US-00031 TABLE 8 LC/MS result of Pro-CNP38 proteolytic
cleavage A210 Protein Conc. Percent Percent Sample Peak Area
(mg/mL) MW4102 MW3115 1 H 55 C. 942 0.04 * 91.8 8.2 2 H 70 0 2878
0.11 * 79.2 20.7 3 H 70 6H 2675 0.10 * 80.6 19.4 4 H 70 24 2741
0.11 * 79.2 20.7 5 H 70 EDTA 2385 0.09 * 80.8 19.2 6 H 70 pH 10
1917 0.07 * 81.2 18.8 7 55 C. 1291 0.05 61.1 38.9 8 70 C. 0 4533
0.18 96.8 3.2 9 70 C. 6H 4120 0.16 97.5 2.5 10 70 C. 24 4108 0.16
96.5 3.5 11 70 C. EDTA 4336 0.17 96.9 3.1 12 70 C. pH 10 3425 0.13
97.5 2.4
All samples (8-12) cleaved at 70.degree. C. showed limited
proteolytic cleavage (less than 4% cleavage of Pro-CNP38). Almost
40% of Pro-CNP38 was cleaved by protease when cleavage was carried
out at 55.degree. C. (sample 7). Basic pH and EDTA did not
influence the non-specific proteolytic cleavage. High temperature
(120.degree. C. for 2 hrs) non-specifically cleaved Pro-CNP38.
It should be noted that the BL21(DE3) strain from Stratagene does
not have the unidentified protease.
Production of Pro-Gly-CNP37 ("Pro-CNP38") and Other CNP Variants
from Different Constructs:
Pro-CNP38 can be produced in large scale in E. coli by means of
overexpression of a TAF-Pro-CNP38 fusion protein as inclusion
bodies followed by formic acid cleavage of the fusion protein.
Following the methods described herein, other TAF-CNP fusion
proteins (TAF-CNP34 and TAF-Pro-CNP53) were expressed as inclusion
bodies and then cleaved with formic acid to generate the CNP
variants CNP34 and Pro-CNP53. FIG. 6 depicts the expression of
TAF-CNP34, FIG. 7 the expression of TAF-Pro-CNP53, FIG. 8 the
products of formic acid cleavage of TAF-CNP34 and TAF-Pro-CNP53,
FIG. 9 the peak for CNP-34 in an LC/MS chromatogram, and FIG. 10
the peak for Pro-CNP53 in an LC/MS chromatogram.
Use of formic acid may result in unspecific cleavage(s) at peptide
bond(s) other than the targeted Asp-Pro bond. To improve the purity
and overall titer of the desired formic acid cleavage product,
different residues of aspartic acid in TAF12 or fragments thereof
were changed to glutamic acid. Moreover, one or more cysteine
residues in TAF12 or fragments thereof were changed to alanine to
prevent unspecific disulfide bond formation. All TAF-Pro-CNP38
fusion proteins having such mutations in TAF12 were expressed as
inclusion bodies and cleaved with formic acid to produce Pro-CNP38.
FIG. 7 shows the expression of TAF-NL-(C/A & 6D/6E)-Pro-CNP38
and TAF(C/A & 10D/10E)-Pro-CNP38, FIG. 11 the expression of
TAF(C/A & 4D/4E)-Pro-CNP38 and TAF(4D/4E)-Pro-CNP38, FIG. 12
the products of formic acid cleavage of TAF(4D/4E)-Pro-CNP38 and
TAF(C/A & 4D/4E)-Pro-CNP38, and FIG. 13 the products of formic
acid cleavage of TAF-NL-(C/A & 6D/6E)-Pro-CNP38 and TAF(C/A
& 10D/10E)-Pro-CNP38. Table 9 summarizes the purity (prior to
purification) and titer of Pro-CNP38 obtained from the various
TAF-Pro-CNP38 constructs.
TABLE-US-00032 TABLE 9 Construct Purity Titer (ug/mL)
pJexpress-TAF-Pro-CNP38 32% 44 pJexpress-TAF(C/A)-Pro-CNP38 41% 50
pJexpress-TAF(4D/4E)-Pro-CNP38 36% 52 pJexpress-TAF(C/A &
4D/4E)-Pro-CNP38 42% 58 pJexpress-TAF(C/A & 10D/10E)-Pro-CNP38*
32% 26 pJexpress-TAF-NL-(C/A & 6D/6E)-Pro-CNP38 50% 55 *Cells
with pJexpress-TAF(C/A&10D/10E)-Pro-CNP38 growed more slowly
and the final cell density (OD.sub.600) was lower compared to other
TAF-Pro-CNP38 constructs.
Large-scale Production of Pro-Gly-CNP37 ("Pro-CNP38") by
Fermentation and Formic Acid Cleavage
BL21(DE3) cells comprising the pJexpress-TAF-CNP construct were
grown in a 10 liter fermenter at 37.degree. C. for about 16-17
hours until OD.sub.600 reached 64. The cells were then
grown/cultured in the presence of 1 mM IPTG at about 35-37.degree.
C., to induce expression of the TAF-Pro-CNP38 fusion protein, for
about 7-8 hours until OD.sub.600 reached 160. The fermentation
produced a titer of 9 g/L TAF-Pro-CNP38. FIG. 14 displays a Western
blot of the TAF-Pro-CNP38 fusion protein produced in the
fermentation.
A cell pellet recovered from 750 mL cell culture from the 10 L
fermentation was resuspended in phosphate-buffered saline, pH 7.4
(PBS) and lysed by three passes through a pressure homogenizer
(10,000 bar). The resulting lysate was centrifuged at 6,500 g for
10 minutes and the supernatant was discarded. The pellet fraction
containing insoluble TAF-Pro-CNP38 fusion protein inclusion bodies
was resuspended in 500 mL PBS with a rotostator. The suspension was
centrifuged at 6,500 g for 10 minutes and the supernatant was
discarded. The resulting inclusion body pellet was resuspended in
500 mL water with a roto stator and incubated at 55.degree. C. for
30 minutes. 250 mL 6% formic acid was added to the warmed inclusion
body suspension for a final concentration of 2% formic acid and
incubated at 55.degree. C. for 20-24 hours. 50 mL of 400 mM
Na.sub.2HPO.sub.4 was added after 20-24 hours to begin the
neutralization of the formic acid cleavage reaction, and the
resulting mixture was titrated with 50% w/v NaOH to pH 6.9-7.4 and
left at room temperature for 30 minutes. Upon neutralization, a
heavy precipitate formed and was removed by centrifugation at 6,500
g for 10 minutes. The supernatant was retained and contained on
average 1.3 g/L culture of 80% pure Pro-CNP38. The majority of the
E. coli and TAF-related proteins and peptides remained in the
pellet.
The soluble Pro-CNP38 resulting from the formic acid cleavage and
neutralized supernatant was 80% pure and contained a mixture of
linear and cyclized Pro-CNP38 peptides along with other
product-related impurities. The pH neutral supernatant containing
Pro-CNP38 was sterile-filtered. Further purification by
anion-exchange chromatography using a Fractogel TMAE Hi-CAP column
(EMD Biosciences) to remove DNA, endotoxins, and peptide
contaminants, which should bind to the column, was performed at pH
7-7.4. The flow-through fraction contained partially cyclized
Pro-CNP38. Cupric sulfate was added to the flow-through fraction to
a final concentration of 10 uM and incubated at room temperature
for 1 hour. The addition of Cu.sup.++ at neutral pH catalyzed the
oxidation of free cysteine sulfhydryl groups on the peptide to form
an intramolecular disulfide bond, resulting in 100% cyclized
Pro-CNP38 and no detectable linear peptide. The conductivity of the
solution was adjusted to <15 mS/cm by the addition of water.
Cation-exchange chromatography using an SP-Sepharose column (GE
Healthcare) and a sodium phosphate buffer (pH 7) was then performed
to remove any remaining DNA and endotoxins in the flow-through and
further purify Pro-CNP38 to about 95-96% purity with <0.5%
non-product related impurities. FIG. 15 is an SDS-PAGE of eluate
fractions from the SP-Sepharose column; the highest concentrations
of Pro-CNP38 were found in fractions 22 to 30. Reverse-phase
HPLC/MS analysis of non-pooled fraction 24 indicated the presence
of 90% Pro-CNP38, 5% Pro-CNP38 with an oxidized methionine residue,
3% Pro-CNP38 cleaved at the Gly-Cys bond to form CNP-17, and 1.6%
Pro-CNP38 cleaved at the Asp-Arg bond in the cyclic domain. The
yield of pure Pro-CNP38 peptide upon final purification was 0.9 g/L
cell culture (36% total recovery).
Pro-CNP38 collected from five separate purifications was pooled for
formulation. The pooled product contained 93.5% Pro-CNP38, 3.3%
Pro-CNP38 with an oxidized methionine residue, 1.3% deamidated
Pro-CNP38, and 1% Pro-CNP38 cleaved at the Gly-Cys bond to form
CNP-17. Samples were diluted with 50 mM sodium phosphate (pH 7) to
a conductivity of 10 mS/cm and loaded onto a CM-Sepharose column
(GE-Healthcare) for concentration and buffer exchange. The weak
cation-exchange property of the CM-Sepharose resin allows peptides
to dissociate from the column with weak acid solutions rather than
typical salt gradients. Acid concentrations required for
dissociation of Pro-CNP38 from the CM-Sepharose column depended
upon column loading. At 50 mg Pro-CNP38 per mL of resin, 10 mM HCl
was sufficient for Pro-CNP38 elution. At 9 mg Pro-CNP38 per mL of
resin, 50 mM HCl was required for elution. When the loading was 9
mg Pro-CNP38 per mL of resin, Pro-CNP38 eluted in less than one
column volume, which significantly concentrated the peptide. The
eluate fraction contained 20.3 mg/mL of 95% pure Pro-CNP38, along
with 3% Pro-CNP38 with an oxidized methionine residue, 1%
deamidated Pro-CNP38, and <1% Pro-CNP38 cleaved at the Gly-Cys
bond to form CNP-17. The concentrated solution of Pro-CNP38 in weak
acid is suitable for dilution into appropriate buffers for either
liquid or lyophilized formulations.
Example 3
Cleavage of CNP Variants by Neutral Endopeptidase In Vitro
To determine the effects of amino acid substitutions, amino acid
extensions, backbone modifications, side chain modifications and
PEGylation on the susceptibility of CNP variants to neutral
endopeptidase (NEP) cleavage, peptide cleavage assays were carried
out using an in vitro assay that monitored disappearance of the
non-cleaved CNP variant.
Recombinant human NEP (1 ug/mL final concentration) was added to
100 uM CNP variant diluted in 0.1 M Tris, pH 7. The reaction
mixture was incubated at 37.degree. C. for various periods of time,
and the reaction was quenched with EDTA (10 mM final) followed by
heat denaturation. The reaction mixture was reduced and then the
reaction products were analyzed using HPLC and mass spectroscopy.
The half-life of the CNP variant was calculated based on the
disappearance of intact CNP variant over time. The results for
digested CNP variants were compared to a parallel wtCNP22 digestion
and normalized to the results for 100 uM CNP22 digested by 1 mg/mL
NEP (t.sub.1/2=80 min).
Table 1 lists the half-lives, based on the in vitro NEP cleavage
assay, of various CNP variants having backbone or side chain
modifications. Removal of three of the six NEP cleavage sites in
Analog L nevertheless resulted in a substantially shorter
half-life. Of tested CNP variants, the greatest resistance to NEP
cleavage was exhibited by Analog N, which contains the D-enantiomer
of all 22 amino acids of CNP22, and by Analog M, which has an
N-methylated amide bond at both Leu9 and Leu11. However, both
Analogs N and M failed to stimulate production of cGMP (see
below).
Comparing the half-lives of Analogs A, B, E, F, G and H to one
another, half-lives were determined to be about 1.5- to about
2.5-fold longer for Analogs E and G compared to those for Analogs
A, B, F and H. All of these six analogs showed resistance or
improved resistance to cleavage at the Cys6-Phe7 bond relative to
wtCNP22 (data not shown). The rank order of analog resistance to
NEP at 1 ug/ml, based on half-life, is Analog G
(3-Cl-Phe).gtoreq.Analog E (D-Phe)>Analog H ("beta-2 Phe"),
Analog B (N-Me-Phe), and Analog F (t-Bu-Gly)=wtCNP22>Analog A
(Cys-CH.sub.2--NH). Analogs E and G have about 1.5 times longer
half-life in comparison to wtCNP22. Besides resistance to cleavage
of the Cys6-Phe7 bond, Analogs B, E, F, G and H also exhibited
resistance to cleavage of the Gly8-Leu9 bond in the presence of 1
ug/mL NEP (data not shown). These results indicate that CNP
variants having backbone or side chain modifications between Cys6
and Gly8 can be resistant to NEP cleavage of the Cys6-Phe7 bond
and/or Gly8-Leu9 bond, but do not necessarily have improved overall
resistance to NEP or a longer half-life than CNP22. The results
seem to be contrary to reports in the literature that NEP first
cleaves at the Cys6-Phe7 bond of CNP22 and then elsewhere.
TABLE-US-00033 TABLE 1 cGMP Response rel. NEP Backbone and Side
Chain to 1 uM CNP22.sup.1 Cleavage Analog Modifications Natriuretic
Peptide 10 nM 1 uM (t.sub.1/2, min) CNP22 (SEQ ID NO: 1) 46 .+-. 10
100 .+-. 13 80.sup.2 N D-CNP22 (all D-amino acids) (SEQ 2 1
>>160 ID NO: 115) A CNP22, C6--CH2--NH (reduced 6 66 55
carbonyl) (SEQ ID NO: 56) B CNP22, N-methyl-F7 (methylated 2 38 80
amide) (SEQ ID NO: 57) BD CNP22, N-methyl-L9 (SEQ ID NO: 2 8 ND
116) BN CNP22, N-methyl-L11 (SEQ ID 10 51 ND NO: 117) BE CNP22,
N-methyl-L20 (SEQ ID 2 5 ND NO: 118) M CNP22, N-methyl-L9,
N-methyl- 1 11 >>160 L11 (SEQ ID NO: 94) K CNP22,
N-methyl-L9, N-methyl- 1 1 80 L20 (SEQ ID NO: 92) L CNP22,
N-methyl-L9, N-methyl- 18 10 30 L11, N-methyl-L20 (SEQ ID NO: 93) J
CNP22, C6--CH2--NH, N- ND ND 50 methyl-L9, N-methyl-L20 (SEQ ID NO:
91) E CNP22, D-F7 (D-Phe) (SEQ ID 2 6 130 NO: 136) H CNP22,
Beta-2-F7 (3-amino-2- 2 2 80 phenylpropionyl) (SEQ ID NO: 57) G
CNP22, 3-chloro-F7 (SEQ ID NO: 17 93 135 137) F CNP22, t-butyl-G8
(SEQ ID NO: 2 18 80 58) V CNP22, K4G, 3,4-dichloro-F7 ND ND 68 (SEQ
ID NO: 119) X CNP22, K4G, 3-methyl-F7 (SEQ ND ND 68 ID NO: 120) ANP
10 23 ND .sup.1Stimulation of cGMP production in NIH3T3 cells by
natriuretic peptide relative to cGMP production in the presence of
1 uM CNP22 .sup.2CNP22 NEP resistance t.sub.1/2 averaged 80 min.
Due to variations in NEP catalytic activity between experiments,
all CNP22 t.sub.1/2 digestions were normalized to 80 min and the
difference coefficient was used to calculate analog t.sub.1/2 in
each experiment to obtain an adjusted t.sub.1/2. ND = Not
Determined
Table 2 lists the half-lives, based on the in vitro NEP cleavage
assay, of various CNP variants having substitutions with natural
and/or unnatural amino acids. Of tested variants, the greatest
resistance to NEP cleavage was shown by Analog BK, which has K4R
and G15S substitutions, and Analog BJ, which has K4R and G15N
substitutions.
TABLE-US-00034 TABLE 2 cGMP Response relative NEP Specificity
Mutations to 1 uM CNP22.sup.1 Cleavage Analog Natriuretic Peptide
10 nM 1 uM (t.sub.1/2, min) CNP22 (SEQ ID NO: 1) 46 .+-. 10 100
.+-. 13 80 AH CNP22, K4R (SEQ ID NO: 35) 59 121 80 BP CNP22, K4R,
G5S 45 ND ND (SEQ ID NO: 121) BO CNP22, K4R, G5R 18 80 ND (SEQ ID
NO: 122) P CNP22, K4G (SEQ ID NO: 123) ND ND 68 Z CNP22, K4R, F7Y 2
18 ND (SEQ ID NO: 95) AB CNP22, K4R, G8S 26 .+-. 26 86 .+-. 17 ND
(SEQ ID NO: 97) AA CNP22, K4R, G8V 3 25 ND (SEQ ID NO: 96) AC
CNP22, K4R, G8T 11 .+-. 2 66 .+-. 16 80 (SEQ ID NO: 98) AD CNP22,
K4R, L9T 4 68 ND (SEQ ID NO: 99) BH CNP22, K4R, K10R 12 80 ND (SEQ
ID NO: 112) BF CNP22, K4R, K10Cit 6 33 ND (SEQ ID NO: 110) BG
CNP22, K4R, K10Q 9 45 ND (SEQ ID NO: 111) BY CNP22, K4R, K10S 16 53
ND (SEQ ID NO: 124) BK CNP22, K4R, G15S 13 .+-. 1 71 .+-. 11 1.60
(SEQ ID NO: 114) BJ CNP22, K4R, G15N 4 41 150 (SEQ ID NO: 113) AE
CNP22, K4R, G15R 0.3 0.3 ND (SEQ ID NO: 100) AF CNP22, K4R, G15Cit
1.4 2 ND (SEQ ID NO: 101) BZ CNP22, K4R, S16Q 42 116 ND (SEQ ID NO:
125) BX CNP22, K4R, M17N 40 .+-. 2 103 .+-. 17 ND (SEQ ID NO: 126)
AG CNP22, K4R, M17V 10 65 ND (SEQ ID NO: 102) BQ CNP22, K4R, G19S
21 63 ND (SEQ ID NO: 127) BR CNP22, K4R, G19R 22 .+-. 6 84 .+-. 10
ND (SEQ ID NO: 128) AJ CNP22, K4R, L20V 0.2 8 ND (SEQ ID NO: 103)
AK CNP22, K4R, L20t-butyl-Ala 1 21 ND (SEQ ID NO: 104) AT CNP22,
GlE, K4E 11 54 60 (SEQ ID NO: 105) BS CNP22, K4R, L20R 11 8 ND (SEQ
ID NO: 129) BT CNP22, K4R, G21S 7 39 ND (SEQ ID NO: 130) BU CNP22,
K4R, G21T 6 21 ND (SEQ ID NO: 131) BW CNP22, K4R, G21R 20 21 ND
(SEQ ID NO: 132) ANP 10 23 ND .sup.1Stimulation of cGMP production
in NIH3T3 cells by natriuretic peptide relative to cGMP production
in the presence of 1 uM CNP22 .sup.2CNP22 NEP resistance t.sub.1/2
averaged 80 min. Due to variations in NEP catalytic activity
between experiments, all CNP22 t.sub.1/2 digestions were normalized
to 80 min and the difference coefficient was used to calculate
analog t.sub.1/2 in each experiment to obtain an adjusted
t.sub.1/2. ND = Not Determined
Table 3 lists the half-lives, based on the in vitro NEP cleavage
assay, of CNP variants having N-terminal and/or C-terminal
modifications, including amino acid extensions. Of the analogs
tested, Analogs AZ, CC, CF, BL, CS, CK and CL, Pro-Gly-CNP27 and
HSA-CNP27 were most resistant to NEP degradation.
TABLE-US-00035 TABLE 3 cGMP Response rel. NEP N- and C-Terminal
Modifications to 1 uM CNP22.sup.1 Cleavage Natriuretic Peptide 10
nM 1 uM (t.sub.1/2, min) CNP22 46 .+-. 10 100 .+-. 13 80 BC
Pentanoic acid (N-term.)-CNP22, G1E (SEQ ID NO: 109) ND ND ND BB
Heptanoic acid (N-term.)-CNP22, G1E (SEQ ID NO: 108) 32 .+-. 4 84
.+-. 19 45-65 AV Pentanoic acid (N-term.)-CNP22, G1E, K4E (SEQ ID
NO: ND ND 120 106) AW Heptanoic acid (N-term.)-CNP22, G1E, K4E (SEQ
ID NO: ND ND <20 107) AX CNP17 (delta N-term) (SEQ ID NO: 2) 18
69 ND R-CNP22 (SEQ ID NO: 40) ND ND ND AZ R-CNP22, K4R (SEQ ID NO:
41) 54 .+-. 11 106 .+-. 15 .gtoreq.160 ER-CNP22 (SEQ ID NO: 38) ND
ND ND BA ER-CNP22, K4R (SEQ ID NO: 39) 38 .+-. 10 113 .+-. 10 90
GANRR-CNP22 (SEQ ID NO: 65) ND ND ND AY GANRR-CNP22, K4R (SEQ ID
NO: 36) 59 .+-. 9 105 .+-. 20 65 GANQQ-CNP22 (SEQ ID NO: 64) ND ND
ND CH GANQQ-CNP22, K4R (SEQ ID NO: 69) 44 .+-. 8 95 .+-. 11 ND
GANPR-CNP22 (SEQ ID NO: 66) ND ND ND CI GANPR-CNP22, K4R (SEQ ID
NO: 37) 50 .+-. 1 105 .+-. 12 ND GANSS-CNP22 (SEQ ID NO: 67) ND ND
ND CG GANSS-CNP22, K4R (SEQ ID NO: 70) 27 .+-. 1 88 .+-. 1 95 CA
AAWARLLQEHPNA-CNP22 (SEQ ID NO: 61) 24 76 ND CB
AAWARLLQEHPNAR-CNP22 (SEQ ID NO: 62) 36 84 ND CC
DLRVDTKSRAAWAR-CNP22 (SEQ ID NO: 63) 34 101 >160 CF
GQPREPQVYTLPPS-CNP22 (IgG1(Fc) fragment) 23 .+-. 9 72 .+-. 19
>160 (SEQ ID NO: 79) PNARKYKGANKK-CNP22 (CNP34) ND ND ND BL
QEHPNARKYKGANKK-CNP22 (CNP37) (SEQ ID NO: 60) 43 .+-. 15 97 .+-. 27
>>160 PQEHPNARKYKGANKK-CNP22 (Pro-CNP37) ND ND ND CE
GERAFKAWAVARLSQ-CNP22 (HSA fragment) 15 87 ND (SEQ ID NO: 81) CY
GQHKDDNPNLPRGANPR-CNP22 (HSA fragment) ND ND ND (SEQ ID NO: 80) CQ
GHHSHEQHPHGANQQ-CNP22 (HRGP fragment) 16 95 ND (SEQ ID NO: 76) CX
GHHSHEQHPHGANPR-CNP22 (HRGP fragment) ND ND ND (SEQ ID NO: 78) CS
GQEHPNARKYKGANPK-CNP22 (modified CNP37) 19 61 >>160 (SEQ ID
NO: 129) CT GQEHPNARKYKGANQK-CNP22 (modified CNP37) 60 121 ND (SEQ
ID NO: 130) CU GQEHPNARKYKGANQQ-CNP22 (modified CNP37) 9 57 ND (SEQ
ID NO: 131) DB GQEHPNARKYKGANKK-CNP22 (Gly-CNP37) 50 .+-. 14 98
.+-. 17 >>160 (SEQ ID NO: 75) PGQEHPNARKYKGANKK-CNP22
(Pro-Gly-CNP37) 49 .+-. 6 103 .+-. 17 >>160 CW
GQEHPNARKYKGANKP-CNP22 (modified CNP37) ND ND ND (SEQ ID NO: 74) CR
GA1-IHPHEHDTHGANQQ-CNP22 (HRGP fragment) 14 .+-. 5 77 .+-. 12 ND
(SEQ ID NO: 128) CZ FGIPMDRIGRNPR-CNP22 (osteocrin "NPR-C
inhibitor") ND ND ND (SEQ ID NO: 82) DA GKRTGQYKLGSKTGPGPK-CNP22
(FGF2 "heparin- ND ND ND binding domain" fragment) (SEQ ID NO: 83)
CK GQPREPQVYTGANQQ-CNP22, K4R (IgG1(Fc) fragment) 2 32 .gtoreq.160
(SEQ ID NO: 84) CL GVPQVSTSTGANQQ-CNP22, K4R (HSA fragment) 3 35
>160 (SEQ ID NO: 85) GHKSEVAHRFKGANKK-CNP22 (HSA-CNP27) (SEQ ID
NO: 144) 51 .+-. 9 109 .+-. 15 >>160 PGHKSEVAHRFKGANKK-CNP22
(Pro-HSA-CNP27) 32 107 ND CN GQTHSSGTQSGANQQ-CNP22, K4R
(fibrinogen) 12 115 ND (SEQ ID NO: 87) CM GQPSSSSQSTGANQQ-CNP22,
K4R (fibronectin) ND ND ND (SEQ ID NO: 86) CO
GSTGQWHSESGANQQ-CNP22, K4R (fibrinogen) 2 33 ND (SEQ ID NO: 88) CP
GSSSSSSSSSGANQQ-CNP22, K4R (zinc finger) ND ND ND (SEQ ID NO: 89)
CD SPKMVOGSG-CNP17-KVLRRH ("BNP tails") 25 102 ND (SEQ ID NO: 68)
CJ RSSCFGGRIDRIGAC ("C-ANP4-23", ANP-derived) ND ND ND (SEQ ID NO:
133) CNP22, K4R, K10R, N.-term.--N-term. dimer/ 19 44 ND
disuccinimidyl glutarate (SEQ ID NO: 134) CNP22, K4R, K10R,
N-term.--N.-term. dimer/Bis-PEO5 19 41 ND (SEQ ID NO: 135) BM CNP53
(SEQ ID NO: 4) 61 101 >>160 ANP 10 23 ND .sup.1Stimulation of
cGMP production in N1H3T3 cells by natriuretic peptide relative to
cGMP production in the presence of 1 uM CNP22 .sup.2CNP22 NEP
resistance t.sub.1/2 averaged 80 min. Due to variations in NEP
catalytic activity between experiments, all CNP22 t.sub.1/2
digestions were normalized to 80 min and the difference coefficient
was used to calculate analog t.sub.1/2 in each experiment to obtain
an adjusted t.sub.1/2. ND = Not Determined
Table 4 lists the half-lives, based on the in vitro NEP cleavage
assay, of CNP variants conjugated to PEG (or PEO) polymers at the
N-terminus. All the PEGylated CNP variants tested and shown in
Table 4 displayed resistance or enhanced resistance to NEP cleavage
except for PEO12-GANPR-CNP22(K4R), which had the same half-life as
wtCNP22. N-terminal PEGylation of CNP22 having a K4G substitution
does not seem to confer substantial improvement in NEP resistance.
For example, PEG2K-CNP22(K4G) was only slightly more resistant to
NEP cleavage than CNP22 (data not shown), whereas PEG2K-CNP22 had a
much longer half-life in vitro than CNP22.
TABLE-US-00036 TABLE 4 cGMP Response rel. to 1 uM NEP N-Terminal
PEGylation CNP22.sup.1 Cleavage Natriuretic Peptide PEG 10 nM 1 uM
(t.sub.1/2, min) CNP22 46 .+-. 10 100 .+-. 13 80 CNP22 PEG20K 0 15
>>160 CNP22 PEG5K 8 .+-. 1 20 .+-. 7 >>160 CNP22 PEG2K
6 .+-. 2 32 .+-. 4 >>160 CNP22 PEO4-(PEO12).sub.3 17 .+-. 1
52 .+-. 6 >>160 (branched) CNP22 PEO24 8 .+-. 5 46 .+-. 10
>>160 (1.2 kDa) CNP22 PEG1K 15 .+-. 3 68 .+-. 17 >160
CNP22 PEO12 12 .+-. 7 57 .+-. 18 160 (0.6 kDa) CNP22 (PEO12)-Biotin
19 81 140 CNP22, K4G (PEO12)-Biotin 10 27 100 CNP22, K4R PEO24 15
56 ND CNP22, K4R PEO12 13 44 ND CNP-17 PEG2K 5 50 >160 R-CNP22,
K4R PEO24 15 .+-. 2 75 .+-. 12 ND (SEQ ID NO: 41) R-CNP22, K4R
PEO12 23 .+-. 2 93 .+-. 19 .gtoreq.60 (SEQ ID NO: 41) ER-CNP22, K4R
PEO24 6 .+-. 2 60 .+-. 10 ND (SEQ ID NO: 39) ER-CNP22, K4R PEO12 20
.+-. 1 92 .+-. 25 ND (SEQ ID NO: 39) GANRR-CNP22, K4R PEG2K 15 .+-.
2 45 .+-. 18 ND (SEQ ID NO: 36) GANRR-CNP22, K4R PEO24 28 .+-. 9 82
.+-. 18 >>160 (SEQ ID NO: 36) GANRR-CNP22, K4R PEG1K 15 .+-.
0.4 56 .+-. 23 >160 (SEQ ID NO: 36) GANRR-CNP22, K4R PEO12 40
.+-. 2 99 .+-. 13 >160 (SEQ ID NO: 36) GANQQ-CNP22, K4R PEO24 16
.+-. 13 73 .+-. 30 ND (SEQ ID NO: 69) GANQQ-CNP22, K4R PEO12 30 78
ND (SEQ ID NO: 69) GANPR-CNP22, K4R PEO24 ND ND ND (SEQ ID NO: 37)
GANPR-CNP22, K4R PEO12 ND ND 80 (SEQ ID NO: 37) GANSS-CNP22, K4R
PEO24 8 .+-. 5 46 .+-. 21 ND (SEQ ID NO: 70) GANSS-CNP22, K4R PEO12
8 .+-. 0.3 52 .+-. 13 ND (SEQ ID NO: 70) .sup.1Stimulation of cGMP
production in NIH3T3 cells by natriuretic peptide relative to cGMP
production in the presence of 1 uM CNP22 .sup.2CNP22 NEP resistance
t.sub.1/2 averaged 80 min. Due to variations in NEP catalytic
activity between experiments, all CNP22 t.sub.1/2 digestions were
normalized to 80 min and the difference coefficient was used to
calculate analog t.sub.1/2 in each experiment to obtain an adjusted
t.sub.1/2. ND = Not Determined
FIG. 16 shows the NEP resistance profile of five N-terminal
PEGylated conjugates of CNP22. The CNP22 peptides conjugated to PEG
(or PEO) polymers of increasing mass exhibited increasing
resistance to NEP degradation. In particular, PEO24-CNP22,
PEG2K-CNP22 and PEG5K-CNP22 were resistant to NEP degradation over
the assay period of 160 minutes.
FIG. 17 displays the NEP resistance profile of CNP variants CNP37
(Analog BL), CNP53 and GANRR-CNP22(K4R) (SEQ ID NO: 36) having an
N-terminal amino acid extension. As can be clearly seen, both CNP37
and CNP53 were resistant to NEP degradation in this in vitro assay,
whereas GANRR-CNP22(K4R) (SEQ ID NO: 36) had the same lability to
NEP hydrolysis as CNP22.
FIG. 18 depicts the NEP resistance profile of CNP17 and
GANRR-CNP22(K4R) (SEQ ID NO: 36) conjugated to a PEG (or PEO)
moiety at the N-terminus. PEGylation of GANRR-CNP22(K4R) (SEQ ID
NO: 36) greatly improved the NEP resistance of this CNP variant,
with PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) being completely
resistant to NEP cleavage over the assay period of 160 minutes.
Increasing the mass of the PEO moiety from about 0.6 kDa (PEO12) to
about 1.2 kDa (PEO24) improved the NEP resistance of PEGylated
GANRR-CNP22(K4R) (SEQ ID NO: 36). PEGylation of GANRR-CNP22(K4R)
(SEQ ID NO: 36) to a monodispersed PEO24 moiety rather than a
polydispersed PEG1K moiety also improved NEP resistance. Finally,
although both PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) and
PEG2K-CNP17 have a similar total mass (keeping in mind that PEG2K
is polydispersed), the former displayed substantially better NEP
resistance.
NEP resistance assays were also performed on wtCNP22 and CNP
variants G-CNP37, GHKSEVAHRFK-wtCNP27 ("CNP27-HSA", SEQ ID NO: 144)
and PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12") (SEQ ID NO: 36). FIG. 19
shows that G-CNP37 and CNP27-HSA were completely resistant to NEP
cleavage, and CNP27-PEO12 exhibited much greater stability to NEP
degradation compared to wtCNP22.
Example 4
CNP Variant Stimulation of cGMP Production in NIH3T3 Cells
To determine the functional activity of CNP variants, the
production of cGMP was measured in NIH3T3 cells exposed to the CNP
variants. Murine NIH3T3 cells express endogenously the CNP
signaling receptor, NPR-B, which shares 98% protein sequence
identity with human NPR-B. NIH3T3 cells were cultured in high
glucose Dulbecco's Modified Eagle Medium supplemented with 10%
fetal calf serum and antibiotics at 37.degree. C. with 5% CO).
Twenty four to 48 hours prior to signaling, cells were passaged to
12-well plates with a density of 2-5.times.10.sup.5 cells per well
at the time of the assay. CNP variants were resuspended in 1 mM HCl
to a stock concentration of 1 mg/mL (455 uM for wtCNP22) and
subsequently diluted to a 30 uM working stock solution with
phosphate-buffered saline (PBS). Ten-fold serial dilutions were
prepared in phosphate-buffered saline. Culture medium was removed
from the cells and replaced with 0.4 mL PBS/Dulbecco's modified
Eagle medium (50/50, v/v) containing 0.75 mM isobutylmethylxanine.
Plates were incubated at 37.degree. C., 5% CO.sub.2 for 15 minutes
before addition of 0.2 mL CNP variant in PBS and continued
incubation at 37.degree. C. for 15 minutes. Reactions were stopped
by the addition of 0.2 mL lysis buffer supplied with the CatchPoint
cGMP assay kit (Molecular Devices), and cGMP production was
determined with the CatchPoint cGMP Assay (Molecular Devices). All
stimulation experiments were performed in duplicate.
Tables 1-4 summarize the ability of CNP variants having backbone or
side chain modifications, amino acid substitutions, N-terminal
amino acid extensions, and/or N-terminal PEGylation, respectively,
to stimulate cGMP production in NIH3T3 cells. In all four tables,
the values for cGMP production in NIH3T3 cells exposed to 10 nM or
1 uM CNP variant are normalized to cell number and cGMP production
in the presence of 1 uM wtCNP22.
Regarding the results in Table 1, only Analog G having 3-Cl-Phe at
position 7 displayed substantially the same NPR-B stimulation
activity at 1 uM as wtCNP22. With respect to Table 2, various CNP
variants with amino acid substitutions, including Analogs AH, BO,
AB, BH, BZ, BX and BR, showed substantially similar NPR-B
stimulation activity as wtCNP22.
Considering the results in Table 3, many CNP variants having
N-terminal and/or C-terminal modifications, including amino acid
extensions, exhibited comparable NPR-B stimulation activity as
wtCNP22. The functional CNP variants include Analog BB, which is
CNP22(G1E) attached to heptanoic acid at the N-terminus, and Analog
CD, which is the cyclic domain of CNP22 ("CNP17" retaining the Cys6
to Cys22 sequence) conjugated to the N-terminal and C-terminal
"tails" of BNP. FIG. 20 illustrates that GANRR-CNP22(K4R) (SEQ ID
NO: 36), CNP37 (Analog BL) and CNP53 all had similar NPR-B
stimulation activity as wtCNP22 in the in vitro assay.
Of note from Table 3 is that among the CNP variants assayed for
both CNP functionality and NEP resistance, Analog AZ
(R-CNP22(K4R)), Analog CC, Analog CF, Analog BL (CNP37), Analog DB
(Gly-CNP37) and GHKSEVAHRFK-CNP27 (HSA-CNP27) (SEQ ID NO: 144) all
had substantially similar NPR-B stimulation activity as CNP22 while
being substantially more resistant to NEP cleavage than CNP22.
With regard to the results in Table 4, nine N-terminal PEGylated
CNP variants at 1 uM stimulated cGMP production to at least about
70% of the level achieved by wtCNP22. Several noteworthy aspects
appear in Table 4. First, N-terminal PEGylation of GANRR-CNP22(K4R)
(SEQ ID NO: 36) with a monodispersed PEO polymer (PEO12 is about
0.6 kDa, PEO24 about 1.2 kDa) resulted in better NPR-B
functionality than that with a polydispersed PEG polymer (PEG1K has
a polymer number average molecular weight (M.sub.n) of around 1
kDa, PEG2K around 2 kDa) (see also FIG. 21). Second, N-terminal
PEGylation of wtCNP22 with a polydispersed PEG polymer of
increasing M.sub.n (PEG1K, PEG2K, PEG5K and PEG20K) or with a
monodispersed PEO polymer of greater mass (PEO12 and PEO24)
correspondingly decreased the NPR-B activation ability of the CNP
variants (see also FIG. 22). Third, PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36), having the N-terminal GANRR (SEQ ID NO: 8) extension,
stimulated greater cGMP production than PEO24-CNP22 and
PEO24-CNP22(K4R). Also of note is that among the N-terminal
PEGylated CNP variants assayed for both CNP functionality and NEP
resistance, PEO12-R-CNP22(K4R), PEO12-GANRR-CNP22(K4R) (SEQ ID NO:
36) and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) all had
substantially similar NPR-B stimulation activity as CNP22 while
being much more resistant to NEP degradation than CNP22.
Among the CNP variants listed in Tables 1-4 and assayed for both
CNP functionality and NEP resistance, Analogs G, BK, AZ, CC, CF, BL
and DB, Pro-Gly-CNP37, HSA-CNP27 (GHKSEVAHRFK-CNP27) (SEQ ID NO:
144), PEG1K-CNP22, (PEO12)-biotin-CNP22, PEO12-R-CNP22(K4R),
PEO12-GANRR-CNP22(K4R) (SEQ ID NO: 36), and PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36) all had substantially similar NPR-B stimulation
activity as wtCNP22 while being substantially more resistant to NEP
cleavage than wtCNP22.
cGMP production assays were also carried out on wtCNP22 and CNP
variants G-CNP37, GHKSEVAHRFK-wtCNP27 ("CNP27-HSA", SEQ ID NO:
144), wtCNP29 and PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12") (SEQ ID
NO: 36). FIG. 23 shows that CNP22 and all the CNP variants assayed
induced production of similar cGMP levels at either low or high
dose of CNP.
Example 5
Binding Specificity for NPR-A, NPR-B and NPR-C
Signaling Competition Assay
To determine the binding specificity of CNP variants for the
clearance receptor NPR-C, a signaling competition assay is carried
out. Expression plasmids for human NPR-B or NPR-C (each purchased
from OriGene) are transiently transfected and receptors are
expressed in HEK293T cells. Forty hours after transfection, NPR-B,
NPR-C and native HEK293T cells are harvested, counted and plated at
a ratio of 1:1 (NPR-B cells:competing cells (either NPR-C or native
HEK293T cells)) in 12-well or 96-well plates. Twenty hours after
plating, cells are processed for the NPR-B/cGMP stimulation assay
described in Example 4. If present, the natriuretic clearance
receptor, NPR-C, is expected to bind and internalize CNP, thereby
reducing the overall CNP concentration available for signaling
through NPR-B, resulting in decreased cGMP production and a shift
in the dose-response curve to the right. A rightward shift in the
dose-response curve has been verified for wtCNP22. CNP variants
having reduced affinity for NPR-C are not expected to induce a
shift, or are expected to induce a smaller shift, in the
dose-response curve to the right. This signaling competition assay
is similar to that previously described by Cunningham (U.S. Pat.
No. 5,846,932; B. Cunningham, EMBO J. 13(11): 2508-2515 (1994); H.
Jin et al., J. Clin. Invest. 98(4): 969-976 (1996)).
The cGMP stimulation activity of wtCNP-22, Pro-Gly-wtCNP37 and ANP
through NPR-B and NPR-A, and their selectivity for NPR-B vs. NPR-C
and for NPR-A vs. NPR-C, were evaluated in signaling competition
assays. NPR-A, NPR-B and NPR-C individually were transiently
tranfected into HEK293T cells. Thirty hours after transfection, the
cells were plated into 96-well plates: (A) 20,000 NPR-B
cells+20,000 mock transfected cells; (B) 20,000 NPR-B cells+20,000
NPR-C cells; (C) 20,000 NPR-A cells+20,000 mock transfected cells;
and (D) 20,000 NPR-A cells+20,000 NPR-C cells. Twenty hours after
plating, the culture media was removed and replaced with serum-free
media:PBS (1:1)+0.75 uM IBMX for 15 minutes. For cGMP signaling
through NPR-B, dose series for ANP, CNP-22 and Pro-Gly-CNP37 were
added and incubated at 37.degree. C. for 12 minutes before the
assay was stopped by cell lysis. For cGMP signaling through NPR-A,
dose series for CNP-22 and Pro-Gly-CNP37 were incubated at
37.degree. C. for 12 minutes, while dose series for ANP were
incubated at 37.degree. C. for 6 minutes (because NPR-A appeared to
be a "faster" guanylyl cyclase than NPR-B, the incubation time for
ANP was shortened in order not to max out (use up all cellular GTP)
too soon when signaling with ANP). FIGS. 24A and B show that CNP-22
and Pro-Gly-CNP37 ("Pro-CNP38") stimulated cGMP production through
NPR-B with similar dose-response curves, and to a much greater
extent than through NPR-A, and exhibited a similar profile for
NPR-B vs. NPR-C selectivity in the signaling competition
assays.
Determination of Binding Affinities (K.sub.i) for NPR-A, NPR-B and
NPR-C
The binding affinities (K.sub.i) of CNP variants for NPR-A, NPR-B
and NPR-C are determined in a heterologous competition binding
assay (U.S. Pat. No. 5,846,932; B. Cunningham, EMBO J. 13(11):
2508-2515 (1994); H. Jin et al., J. Clin. Invest. 98(4): 969-976
(1996)). Membranes from HEK293 cells, or another suitably
transfectable cell line (e.g., HeLa cells), expressing human NPR-A,
NPR-B or NPR-C are prepared for radio-labeled ligand binding
assays. Membrane preparations are diluted in an appropriate buffer
and varying concentrations of wtCNP22 or CNP variant (competitor)
are added with I.sup.125-labeled wtCNP22 (Bachem). Samples are
incubated at room temperature to allow for ligand/receptor
equilibration and bound peptide is separated from free peptide by
filtration through PVDF filter membranes. Filters are washed before
the addition of scintillant and counting by a scintillation
counter. Binding is measured in duplicate for each concentration of
competitor peptide. CNP variant affinity (K.sub.i, equilibrium
dissociation constant) and B.sub.max, (receptor number) are
calculated by non-linear regression analysis and/or the
Cheng-Prusoff equation.
CNP variants exhibiting reduced affinity to NPR-C are expected to
have reduced susceptibility to clearance by NPR-C and thus a longer
plasma or serum half-life. Increased half-life of the CNP variants
in circulation would increase the availability of the variants for
therapeutic activity.
Example 6
Effect of CNP Variants on the Growth of Rat Chondrosarcoma (RCS)
Cells and cGMP Production in RCS Cells
To assess the ability of CNP variants to stimulate bone growth,
skeletal dysplasia is simulated in cell culture by treating rat
chondrosarcoma (RCS) cells with fibroblast growth factor 2 (FGF-2),
which activates fibroblast growth factor receptor 3 (FGFR-3) and
induces growth arrest (Krejci et al., J. Cell Sci.,
118(21):5089-5100 (2005)).
Optimal CNP treatment parameters are determined by varying CNP
concentration (0.05, 0.1, 0.2 and 0.5 uM), and treatment duration
and interval (continuous; 2.5 min, 10 min, 30 min, 1 hr, 2 hr, 4 hr
and 8 hr once a day; 2.5 min, 10 min, 30 min, 1 hr, 2 hr and 4 hr
twice a day). After 72 hours, cells are counted using an automated
cell counter, and the amount of extracellular matrix is estimated
using alcian blue staining.
RCS cells are then treated with a CNP variant using the optimal
conditions determined from the growth experiments with wtCNP22. The
concentration of cGMP is measured by competitive ELISA for
untreated RCS cells, RCS cells treated with CNP, and RCS cells
treated with the CNP variant. Cell growth and matrix synthesis
resulting from treatment with the CNP variant are also monitored
and compared to those resulting from CNP treatment.
To assess the effect of CNP variants in a human cell culture
system, primary re-differentiated human chondrocytes in alignate
beads are treated with wtCNP22 and CNP variants, and cGMP
concentration is determined by competitive ELISA as a measure of
effective CNP signaling.
The methods described herein can be employed to assess the ability
of CNP variants to stimulate cGMP production in and growth of rat
chondrosarcoma cells in vitro.
Example 7
Dose Response Study in Rat Chondrosarcoma Cells
The tyrosine kinase receptor fibroblast growth factor receptor 3
(FGFR-3), a negative regulator of chondrocyte growth, is
contitutively on in achondroplasia subjects. Stimulation of the
FGFR-3 receptor with FGF-2 causes growth arrest by prolonged
activation of Erk MAPK, and causes decreased matrix synthesis and
loss of matrix, as well as a change in cell shape. Continuous
exposure of rat chondrosarcoma (RCS) cells to fibroblast growth
factor 2 (FGF-2) simulates achondroplasia in cell culture by
activating FGFR-3 and inducing growth arrest (Krejci et al., J.
Cell Sci., 118(21): 5089-5100 (2005)). To determine the dose of CNP
variant and frequency of dosing that stimulate sufficient growth of
bone cells, a dose response study was performed using the RCS cell
assay as described in Example 6.
RCS cells were seeded at 10.times.10.sup.3 cells per well in
24-well plates, grown for 24 hr, treated for 72 hr, and then
counted. RCS cells were continuously exposed to FGF-2 (5 ng/mL) to
simulate a constitutively active FGFR-3, which induced cell growth
arrest (see bar #5 in FIG. 25). Wild-type CNP22 (0.2 uM) was
cultured continuously (72 hr), 1 hr daily or 2 hr daily. All
stimulants were changed daily Continuous exposure of RCS cells to
0.2 uM CNP22 in the presence of 5.0 ng/mL FGF-2 partially reversed
FGF2-induced growth arrest, leading to the growth of approximately
200.times.10.sup.3 cells per well (bar #6 in FIG. 25), compared to
approximately 100.times.10.sup.3 cells per well in the absence of
CNP22 (bar #5 in FIG. 25).
Both 1 hr exposure to CNP22 (0.2 uM) once a day and 2 hr exposure
to CNP22 (0.2 uM) once a day achieved about 84% of the effect of
continuous CNP22 (0.2 uM) exposure on chondrocyte growth (bars #7
and 8 in FIG. 25). These results demonstrate that continuous
exposure of growth-arrested chondrocytes to CNP22 is not required
for reversal of cell growth arrest. Additionally, dose response
studies demonstrate that lower doses of CNP22 are capable of
reversing growth arrest (FIG. 26A).
Furthermore, histological and cell morphological analysis of the
extracellular matrix showed that CNP22 treatment antagonized
FGF2-mediated loss of chondrosarcoma extra-cellular matrix and
increased matrix synthesis. Exposure to FGF-2 decreased matrix
synthesis and increased degradation, while addition of CNP22 to the
FGF-2 cell culture increased matrix synthesis and partially
inhibited FGF-2, as assessed by .sup.35S-sulfate and .sup.3H-Pro
incorporation into, or decrease from, matrix (FIGS. 27A-D).
Analysis of aggrecan and fibronectin production (mRNA and protein)
in RCS cells cultured with FGF-2 and CNP22 shows that FGF-2
decreased aggrecan level and increased fibronectin level, which was
inhibited by addition of CNP22 (FIGS. 28A-C). FGF-2 induces and
activates matrix-processing molecules predominantly via Erk, and
addition of CNP22 shows some effect on this activation.
Additional high-throughput assays for measuring growth arrest, such
as crystal violet staining, are useful for measuring the effects of
CNP22 and variants thereof on RCS cells.
Similar dose response studies can be conducted with the CNP
variants described herein to determine their effective dose for
reversing FGF2-induced growth arrest of RCS cells.
Example 8A
Ex Vivo Stimulation of Growth of Tibia and Femur from Mice with
Mild Achondroplasia
Mouse tibial organ culture model has been used to demonstrate the
efficacy of wild-type CNP22 in stimulating longitudinal bone
growth. Treatment of wild-type tibiae with CNP22 at 10.sup.-8,
10.sup.-7 or 10.sup.-6 M for 6 days increased longitudinal growth
by 31%, 40% and 42%, respectively. Histological evaluation also
showed expansion of the hypertrophic zone, e.g., an increase in the
number and size of hypertrophic chondrocytes in the growth plate
(Agoston et al., BMC Dev. Biol. 7:18 (2007)). Similar findings were
observed in tibiae isolated from FGFR3.sup.Ach mice (Yasoda et al.,
Nat. Med. 10: 80-86 (2004)).
To determine the efficacy of CNP variants in stimulating
longitudinal bone growth, CNP variants were tested in a mouse organ
culture model of endochondral bone growth in wild-type mice and
transgenic mice having a G380R mutation in the human FGFR-3 gene
(FGFR3.sup.wt/Ach heterozygote) which represent a mouse model of
mild achondroplasia. In brief, the pharmacological activity of
wild-type CNP22 and CNP variants was compared in an organ culture
model of embryonic or neonatal mouse tibiae, isolated from
wild-type and FGFR3.sup.wt/Ach littermates. Overall bone growth and
histological changes within the growth plate were assessed.
Conditioned culture medium is also assessed for biomarkers of
intracellular signaling (cGMP), cartilage metabolism (type II
collagen, other collagens, aggrecan chondroitin sulfate), bone
metabolism (bone alkaline phosphatase, osteocalcin, type I collagen
[C-telopeptide, N-telopeptide]), and inflammation (interleukin-1,
interleukin 6, interleukin-11).
Effective CNP variants are identified by their ability, e.g., to
stimulate production of cGMP, and bone growth as measured by
increased longitudinal bone length and expansion of the cells in
the hypertrophic zone of the growth plate.
Measurement of Bone Growth
The efficacy of wtCNP22, CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36) in stimulating longitudinal femoral growth was evaluated in
the mouse organ culture model. For these experiments femora were
isolated from 2-3 day old wild-type mice and cultured in alphaMEM
supplemented with 0.2% BSA, 0.5 mM L-glutamine, 40 units
penicillin/mL and 40 ug streptomycin/mL, for 8 days in the presence
of vehicle, CNP22 or CNP variants. The treatment commenced at day 0
and was repeated every two days thereafter, as the medium was
changed. Bones were measured prior to treatment and every two days
thereafter, using a dissection microscope fitted with a 1 cm
eye-piece reticule. Conditioned medium was used for biomarker
analysis. At day 8 bones were fixed in 4% paraformaldehyde for 24
hr, decalcified in 5% formic acid for 24 hrs, dehydrated and
embedded in paraffin. Bones were sectioned at 5 um (microns), which
were then deparaffinized, rehydrated, and stained with Alcian Blue
for 30 min (pH 2.5; MasterTech). Alcian Blue stains cartilage blue.
Stained sections were visualized and photographed by brightfield
microscopy. The thickness of the hypertrophic region of the growth
plate cartilage was determined by image analysis.
FIG. 29 illustrates the effect of wtCNP22, CNP37 and
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) on longitudinal growth of
3-day old wild-type mouse femurs treated with the CNP peptides
every two days. The results were normalized to measurements prior
to treatment (day 0). The studies were performed in triplicate
(vehicle) or quadruplicate (CNP peptides). As shown in FIG. 29,
CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36), as well as CNP22,
were effective in stimulating longitudinal femoral growth, with the
N-terminal PEGylated CNP variant being the most effective.
The growth of wild-type and FGFR3.sup.ach mouse femur and tibia in
response to CNP22, CNP37 and PEO24-GANRR-CNP22(K4R) ("CNP27-PEO24")
(SEQ ID NO: 36) was also assessed. Culture of either wild-type or
achondroplastic (FGFR3.sup.ach) mouse tibia showed that CNP37 and
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) both increased the
longitudinal growth of the tibia compared to vehicle or CNP22
(FIGS. 30 and 31). CNP22, CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36) also stimulated the growth of wild-type mouse femur (FIG.
32). Moreover, each of CNP22, PEO24-GANRR-CNP22(K4R) (SEQ ID NO:
36) and CNP37 increased the longitudinal growth of FGFR3.sup.ach
mouse femur compared to vehicle (FIG. 33).
Furthermore, ex vivo distribution of CNP37 in the growth plate of
FGFR3.sup.ach mouse tibia was evaluated. Bone samples were prepared
as described above. Paraffin sections were cut and heat fixed for 1
hour at 60.degree. C. Antigen retrieval with 1% hyaluronidase at
37.degree. C. (30 minutes) was followed by a 1 hour serum block
(10% Normal Goat Serum). CNP22 antibody (1:500 dilution; Peninsula
Laboratories Inc., San Carlos, Calif.) was applied overnight at
4.degree. C. For immunodetection, Vectastain ELITE ABC kit (Vector,
Burlingame, Calif.) was used according to the manufacturer's
recommendations. Specific bound peroxidase was visualized by
incubation with DAB substrate kit (Vector) and the reaction was
developed for 3 minutes. Slides were then dehydrated and mounted
and photographed using a brightfield microscope. Staining for CNP
in the growth plate of FGFR3.sup.ach mouse tibia showed that CNP
immunoreactivity was increased in the regions of articular and
hypertrophic chondrocytes (FIG. 34), indicating that CNP37 was
delivered to the chondrocytes.
In addition to the distribution of CNP variants in the bone growth
plate, the ex vivo effects of CNP37, CNP22 and vehicle on cells in
the FGFR3.sup.ach and wild-type growth plate, e.g., hypertrophic
cell size and cellularity of proliferating zone, were also
evaluated. Bone samples were prepared, including Alcian Blue
staining, as described above. Images of the entire proximal growth
plate were taken at 4.times. magnitude. The growth plate is divided
into three zones, starting from the epiphyseal side of cartilage:
the resting zone (individual small chondrocytes), proliferating
zone (columns of stacked chondrocytes parallel to the long axis of
the bone), and hypertrophic zone (large chondrocytes and thin septa
between the chondrocytes). In these regions, measurements were made
by ImageJ software, including the number of proliferating
chondrocytes per column and the density of hypertrophic
chondrocytes. A test square (4.times.4 mm.sup.2) at five different
regions of the hypertrophic zone was used to determine the density
of hypertrophic chondrocytes. The cell size of hypertrophic
chondrocytes was calculated by 1 over determined cell density.
Cellularity of proliferating columns was increased by CNP37 and
CNP22 in both wild-type and FGFR3.sup.ach mice (FIGS. 35B and C).
Chondrocyte hypertrophy in FGFR3.sup.ach mice was also increased as
a result of culture with CNP22 or CNP37 (FIGS. 36B and C).
Ex vivo studies of cultures of mouse bones indicated that CNP37 was
delivered to the growth plate and was able to increase chondrocyte
cellularity and hypertrophy, which are associated with growth plate
expansion and longitudinal bone growth. To assess the
biodistribution of CNP37 in the bone growth plate in vivo and the
in vivo effects of CNP37 on the growth plate (including total
growth plate thickness, hypertrophic zone thickness, and
cellularity of proliferating zone), bone samples were obtained from
FGFR3.sup.ach mice treated with vehicle or CNP37 as described
above. For biodistribution and in vivo effects studies, tibias were
fixed and stored in 70% ethanol. For immunohistochemistry, the
samples were decalcified in 5% formic acid for 2 days, dehydrated
and embedded in paraffin. Bones were sectioned at 5 um (microns),
which were then deparaffinized, rehydrated, and used for CNP
immunohistochemistry as described above. For cellular image
analysis, bones were sectioned at 5 (microns), and then
deparaffinized, rehydrated, and stained with Alcian Blue for 30
minutes (pH 2.5; MasterTech) and Hematoxylin & Eosin for 30
seconds. Stained sections were visualized and photographed by
brightfield microscopy. Thicknesses of the growth plate and the
proliferating and hypertrophic zones were measured using ImageJ
software.
In vivo biodistribution studies demonstrated that, similar to the
ex vivo studies, CNP immunoreactivity was increased in the regions
of articular and hypertrophic chondrocytes in the tibia growth
plate of FGFR3.sup.ach mice treated with CNP37, indicating that
CNP37 was delivered in vivo to the growth plate of FGFR3.sup.ach
mouse tibia (FIG. 37). Furthermore, CNP37 treatment significantly
increased the total growth plate thickness, proliferating zone
thickness and hypertrophic zone thickness of FGFR3.sup.ach mouse
tibia in vivo (FIGS. 38A-C).
These results demonstrate that CNP variants of the disclosure
penetrate into the growth plate of wild-type and achondroplastic
animals, increase the number and size of chondrocytes, increase the
thickness of the proliferating zone and the hypertrophic zone of
the growth plate, and increase longitudinal bone growth in treated
wild-type and achondroplasic animals. Therefore, the CNP variants
are useful for stimulating bone growth in achondroplastic
subjects.
Measurement of Biomarkers
In addition to measurement of bone growth in response to CNP
variants, assay of the levels of biomarkers for cartilage and bone
formation and growth induced in response to CNP variants is useful
for evaluating the effect of CNP variants on bone growth.
Femurs and tibias were isolated from wild-type and FGFR3.sup.ach
mice as described above. Bones were cultured with CNP22 or a
variant thereof for eight days with the replacement of media every
two days. On the eighth day, media was collected and analyzed for
the biomarkers cGMP (cyclic guanosine 3', 5' cyclic monophosphate)
and fragments of cleaved collagen type II, a cartilage-specific
marker for cartilage turnover. Both markers were measured using
commercially available enzyme-linked immunosorbent assays (ELISA)
for cGMP (Cayman Chemical Co., Ann Arbor, Mich.) and cleaved
collagen type II (Cartilaps) (Immunodiagnostic Systems, Fountain
Hills, Ariz.), following the manufacturer's protocol.
Levels of cGMP and collagen type II fragments were measured from
cell culture extracts after exposure to CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) ("CNP27-PEO24") (SEQ ID NO: 36). FIGS. 39-42
show a large increase (p<0.01) in the levels of cGMP in the
media after exposure of ex-planted wild-type and FGFR3.sup.ach
mouse femurs and tibias to CNP22, CNP37 or PEO24-GANRR-CNP22(K4R)
(SEQ ID NO: 36). In addition, exposure of wild-type and
FGFR3.sup.ach mouse femurs to CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) increased the levels of
cleaved collagen type II, with the treated FGFR3.sup.ach mouse
femurs showing significantly increased (p<0.05) levels of
collagen type II fragments (FIG. 43). Elevated levels of collagen
type II fragments indicate a turnover of the cartilage matrix, and
cartilage turnover typically precedes new bone formation in growing
bones.
Example 8B
Ex Vivo Stimulation of Growth of Femur from Mice with Severe
Achondroplasia
The effect of a CNP variant on the growth of bones from mice with
severe achondroplasia was evaluated ex vivo. Used in the study were
transgenic mice expressing a human FGFR-3gene having a Y367C
mutation (FGFR3.sup.Y367C) [S. Pannier et al., Biochim. Biophys.
Acta, 1792(2): 140-147 (2009)], which represent a mouse model of
severe achondroplasia. Femurs were isolated at embryonic day 16.5
and cultured for 6 days in the presence of 1 uM Pro-Gly-CNP37. Bone
lengths were measured at Day 1 and Day 7. The bones were then
paraffin-embedded, sectioned and stained with hematoxylin and eosin
to assess histological changes and cellular morphology. Treatment
of bone explants isolated from FGFR3.sup.Y367C mice with
Pro-Gly-CNP37 ("ProCNP38") resulted in increase in bone growth and
expansion in the growth plate (FIG. 44). Femurs from
FGFR3.sup.Y367C mice treated with vehicle for 6 days showed an 18%
deficiency in growth length-wise compared to wild-type femurs
treated with vehicle. Treatment of femurs from FGFR3.sup.Y367c mice
with 1 uM Pro-Gly-CNP37 for 6 days reduced the growth deficiency to
just 11%, i.e., reduced the growth defect by around 40%.
Example 9
Serum/Plasma Stability of CNP Variants In Vitro
In preparation for pharmacokinetics (PK) studies, the stability of
CNP variants in serum and/or plasma is evaluated.
Briefly, the analyte is isolated by the removal of serum or plasma
proteins by either a 2% trichloroacetic acid precipitation or a 1:3
serum:acetonitrile precipitation. The precipitation mixture is
vortexed at 14,000 rpm for five minutes, and a portion of the
supernatant is removed and diluted with water prior to transfer to
a silanized autosampler vial for analysis. Serum extracts are then
analyzed by reverse-phase high performance liquid chromatography
(RP-HPLC) with electrospray ionization mass spectrometry (ESI-MS).
A single mass (m/z), shown to be specific for the CNP variant, is
monitored for quantitation purposes.
Initially, analytical stability and recovery is determined.
Analytical (RP-HPLC and ESI-MS) parameters are optimized through
the analysis of matrix standards (serum extracts fortified with
analyte post-precipitation). After optimization, analytical
recovery is determined by spiking serum samples at known
concentrations and comparison of the analyte response to that of
matrix standards prepared at similar concentrations. Analyte
stability in serum extracts is also determined to assure no
significant losses occur after serum precipitation and prior to
actual analysis. To test the effect of freezing on serum stability,
a two-cycle freeze/thaw study is also performed. In this study a
serum sample is spiked with CNP variant and analyzed prior to
freezing overnight at -20.degree. C. The sample is then thawed at
room temperature and re-analyzed. The process is repeated for a
second freeze/thaw cycle.
Serum stability of CNP variant is determined by spiking of
serum/plasma samples with CNP variant at a concentration of 10
ug/mL. The sample is placed in a 37.degree. C. water bath for a
period of three hours. At 30 minute intervals duplicate aliquots of
serum are removed and analyzed. If rapid losses of analyte are
evident (>50% in 30 minutes), the study may be repeated with 10
minute timepoints.
In an exemplary method for determining the stability of CNP
variants in murine plasma, a mixture of CNP variant (10 uL of a
stock solution of about 2.5-5.0 mg/mL), heparinized murine plasma
(50 uL, Bioreclamation, CD-1 Lith Hep 62231), and 5 M NaCl (10 uL)
is incubated at 37.degree. C. and 5% CO.sub.2 for 0-5 hr, and then
quenched with 10.times. protease inhibitor cocktail (15 uL, Sigma
P2714). For extraction, 150 uL of MeOH/0.1% FA is added to 85 uL of
the reaction mixture, and the resulting mixture is vortexed for 1
min and then centrifuged at 15.degree. C. for 15 min. 75 uL of the
supernatant is added to 300 uL of aqueous 0.1% FA. A small portion
of the resulting mixture is subjected to analysis by LC/MS.
Example 10
Pharmacokinetics and cGMP Production in Rats and Mice
Studies were conducted in normal rats to evaluate the
pharmacokinetics (PK) profile of CNP22 and certain CNP variants and
the time courses of plasma cGMP concentration after single
intravenous (i.v.) or subcutaneous (s.c.) administration of the CNP
peptides. Plasma CNP immunoreactivity was determined by using a
competitive radioimmunoassay (RIA) with an anti-CNP rabbit
polyclonal antibody. Plasma cGMP concentration was determined by
RIA using a commercially available kit (YAMASA cyclic GMP Assay
kit, YAMASA Corporation).
Normal male rats, 7-8 weeks of age, were used. Recombinant
wild-type CNP22, CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36)
were evaluated. A dosage of 20 nmol/kg of each CNP peptide as a
solution in 5% mannitol was intravenously injected once into the
tail, or a dosage of 50 nmol/kg of each CNP peptide as a solution
in 0.03 mol/L acetic acid buffer solution, pH 4.0, containing 1%
(w/v) benzyl alcohol and 10% (w/v) sucrose, was subcutaneously
injected once into the back.
Plasma CNP immunoreactivity was determined by the competitive RIA
using anti-CNP rabbit polyclonal antibody. Standard and QC samples
were prepared. Fifty uL of the standard, QC and assay samples were
added, respectively, to test tubes containing 50 uL of RIA buffer.
Diluted anti-CNP rabbit polyclonal antibody (100 uL) was added to
the tubes. All tubes were kept at 4.degree. C. overnight.
.sup.125I-[Tyr.sup.0]-CNP22 solution (100 uL) and rabbit IgG
solution (100 uL) were added and left at approximately 4.degree. C.
overnight. One milliliter of anti-rabbit IgG goat serum containing
10% polyethylene glycol was added, vortexed and left at
approximately 4.degree. C. for at least 1 hour, and then the
insoluble fraction was precipitated by centrifugation. After
aspiration of the supernatant, the amount of radiation (gamma line)
in the sediment was measured by a gamma-counter. Each sample was
measured in duplicate, and the mean was adopted as the value
determined.
Plasma cGMP concentrations in the sample at 5, 30, 60 and 90
minutes after i.v. dosing, or at 5, 30, 60, 120 and 180 minutes
after s.c. dosing, were determined by the competitive RIA using
anti-cGMP monoclonal antibody. Standard samples were prepared. 100
uL of the assay samples (standard solutions for the calibration
curve or the diluted plasma samples for cGMP determination) were
transferred to test tubes. Then 100 uL of anti-cGMP monoclonal
antibody solution and 100 uL of .sup.125I-labeled succinyl cGMP
tyrosine methyl ester solution were added to the tubes,
respectively. All tubes were kept at 4.degree. C. overnight. After
the addition of 500 uL dextran charcoal solution, the tubes were
vortexed and then placed on ice for 10 minutes. The reaction
mixture was centrifuged and 500 uL of the supernatant was
transferred from each sample to a new test tube. The amount of
radiation (gamma line) in the supernatant was measured by a
gamma-counter. Each sample was measured in duplicate, and the mean
was adopted as the value determined.
Plasma CNP immunoreactivity was employed for pharmacokinetics (PK)
analysis. PK analysis was performed using WINNONLIN.RTM.
Professional (Pharsight Corporation). The PK profiles of CNP22,
CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) after i.v.
administration were calculated using PK parameters such as
concentration at 0 hour (C.sub.0: extrapolation, pmol/mL), total
body clearance (CL.sub.tot: mL/min/kg), distribution volume at
steady state (V.sub.dss: mL/kg), area under the plasma
concentration-time curve (AUC: pmolmin/mL), mean residence time
(MRT: min), and half-life (T.sub.1/2: min). The PK profiles of the
CNP peptides after s.c. administration were calculated using PK
parameters such as maximum plasma concentration (C.sub.max:
pmol/mL), time to reach C.sub.max (T.sub.max: min), area under the
plasma concentration-time curve (AUC: pmolmin/mL), mean residence
time (MRT: min), and half-life (T.sub.1/2: min).
In plasma spike recovery experiments, the RIA detected CNP22, CNP37
and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) similarly (data not
shown).
Procedures similar to those described above are employed to study
in mice the PK profiles of CNP22 and variants thereof and their
ability to stimulate cGMP production.
The PK profiles of CNP22, CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID
NO: 36) after i.v. administration in three rats are illustrated in
FIG. 45. As shown by FIG. 45, CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ
ID NO: 36) had a much longer half-life and a much greater
bioavailability than CNP22. The half-life, T.sub.1/2 (min), was
1.42 (.+-.0.45) for CNP22, 22.3 (.+-.1.5) for
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36), and 49.5 (.+-.28.0) for
CNP37. The area under the curve, AUC (pmolmin/mL), was 320 (.+-.54)
for CNP22, 1559 (.+-.568) for CNP37, and 2084 (.+-.424) for
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36).
The PK profiles of the three CNP peptides after s.c. administration
in three rats are depicted in FIG. 46. Compared to CNP22,
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) had a much longer half-life
(78.1 min (.+-.16.4) vs. 10.0 (.+-.5.0)) and a much greater
bioavailability (60% (.+-.6%) vs. 19% (.+-.9%)).
The time courses of plasma cGMP concentrations after i.v.
administration of the three CNP peptides in three rats are
displayed in FIG. 47. FIG. 47 clearly demonstrates that i.v.
administration of CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36)
resulted in much higher plasma levels of cGMP at 30, 60 and 90
minutes than i.v. administration of CNP22.
The time profiles of plasma cGMP concentrations after s.c.
administration of the three CNP peptides in three rats are shown in
FIG. 48. Subcutaneous administration of PEO24-GANRR-CNP22(K4R) (SEQ
ID NO: 36) and CNP37 also resulted in substantially higher plasma
concentrations of cGMP than s.c. administration of CNP22, with the
difference relative to CNP22 increasing over time for
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36), but decreasing over time
for CNP37.
The rat studies indicate that compared to wtCNP22, the CNP variants
CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) had a
substantially longer half-life in vivo, had a substantially greater
bioavailability in vivo, and stimulated substantially higher levels
of cGMP production in vivo for an extended period of time. The
resistance of CNP37 and PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) to
NEP degradation correlates to a longer plasma half-life in vivo,
which in turn correlates to prolonged NPR-B/cGMP signaling in vivo.
These results show that compared to CNP22, the CNP variants of the
disclosure, administered by i.v. or s.c. injection (e.g., once
daily), can be more effective in treating CNP-responsive conditions
or disorders, such as bone-related disorders and vascular smooth
muscle disorders.
Example 11
Pharmacokinetics Study in Mice
To determine CNP variants having increased NEP resistance, for
efficacy study in FGFR3.sup.ach mice (see Example 13), a
pharmacokinetics (PK) study is carried out that compares the
pharmacokinetics properties of CNP variants to wild-type CNP22. The
FGFR3.sup.ach mouse is a mutant mouse model of mild achondroplasia,
containing a single transgene on a background of FVB mice.
Wild-type CNP22 or variant thereof is administered as a single
intravenous (i.v.) dose in 6-week old wild-type FVB mice. Exemplary
PK studies were conducted using wtCNP22. Six-week old FVB/N mice
were intravenously administered wtCNP22 in a single dose at 100
nmol/kg. Mean plasma levels of CNP22 were calculated, and the
estimated half-life of CNP22 was determined to be from 0.76 min to
1.03 min.
CNP variants displaying greater resistance to NEP degradation are
expected to exhibit increased serum concentrations over time and a
longer half-life in vivo.
Example 12
Efficacy of CNP Variants in Wild-Type Mice
The in vivo effects of CNP variants on bone growth were assessed in
wild-type mice. Three week old FVB wild-type male mice received
daily subcutaneous (s.c.) injection of either vehicle, G-CNP37 (200
nmol/kg) or PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12") (SEQ ID NO: 36)
(200 nmol/kg) for 5 weeks. Body weight was measured at least once
weekly. Tail length was measured at least once weekly using digital
caliper readings, and body length (naso-anal length), bone length
(tibia, femur, ulna and humerus), skull length (anterior to
posterior cranial segment) and lumbar vertebrae 5 (LV5) length were
measured after 5 weeks of treatment using a caliper. X-rays were
taken at baseline and after 5 weeks of treatment.
Five weeks of treatment of wild-type mice with G-CNP37 resulted in
significant body weight gain, with increased body weight being
observed beginning at Day 9 (p<0.05) (FIG. 49). Treatment with
G-CNP37 also resulted in significantly increased tail length,
beginning at the second week after treatment (p<0.01) (FIG.
50).
Table 10 shows the percentage change in tail length, body
(naso-anal) length, skull (anterior to posterior cranial segment)
length, bone (femur, tibia, humerus and ulna) lengths, and lumbar
vertebrae 5 (LV5) length in wild-type mice injected s.c. once daily
with 200 nmol/kg of either G-CNP37 or PEO12-GANRR-CNP22(K4R)
("CNP27-PEO12") (SEQ ID NO: 36) for 5 weeks, relative to a value of
100% for wild-type mice treated with vehicle only.
TABLE-US-00037 TABLE 10 Anterior to Naso- Posterior Tail Anal
(Skull) Femur Tibia Humerus Ulna LV5 G-CNP37 119%** 114%** 104%*
109%** 105%** 104%** 101% 108%* CNP27- 101% 104%** 101% 102%* 100%
102%* 96%* 103% PEO12 **p < 0.01, *p < 0.05
Treatment with G-CNP37 resulted in significantly increased tail
length, body (naso-anal) length, skull length, proximal bone (femur
and humerus) length, distal bone (tibia) length, and vertebral
(lumbar vertebrae 5) length compared to treatment with vehicle.
Lower doses of Pro-Gly-CNP37 dosed daily s.c. at 5 nmol/kg, 20
nmol/kg or 70 nmol/kg for five weeks resulted in a dose-dependent
increase in tail length, body (naso-anal) length, and bone lengths
compared to vehicle. Table 11 shows the percentage change in tail
length, body (naso-anal) length, and bone (femur, tibia, humerus
and ulna) lengths in wild-type mice injected s.c. once daily with 5
nmol/kg, 20 nmol/kg or 70 nmol/kg Pro-Gly-CNP37 for 5 weeks,
relative to a value of 100% for wild-type mice treated with vehicle
only.
TABLE-US-00038 TABLE 11 Naso- Tail Anal Femur Tibia Humerus Ulna 5
nmol/kg 107.6%** 102.7%** 103.3%** 101.7%** 101.1% 100.9%* 20
nmol/kg 107.8%** 107.6%** 107.1%** 103.4%** 102.3%** 102.5%** 70
nmol/kg 113.6%** 112.5%** 109.3%** 105.9%** 103.6%** 104.1%** **p
< 0.01, *p < 0.05
In another study, various dosing regimens of Pro-Gly-CNP37 were
administered for nine weeks followed by one week of recovery.
Wild-type FVB mice were dosed s.c. with: (1) vehicle daily for nine
weeks, followed by one week of recovery; (2) 20 nmol/kg
Pro-Gly-CNP37 daily for one week, followed by three doses per week
for eight weeks and one week of recovery; (3) 20 nmol/kg
Pro-Gly-CNP37 on alternating weeks; or (4) 5 nmol/kg Pro-Gly-CNP37
daily for nine weeks followed by one week of recovery.
Increased growth in tail length, body length, and bone lengths was
observed in all treatment groups (Groups 2, 3 and 4) at the end of
the study. Table 12 shows the percentage change in tail length,
body (naso-anal) length, and bone (femur, tibia, humerus and ulna)
lengths in wild-type mice administered Pro -Gly-CNP37 ("Pro-CNP38")
under the dose regimens described above, relative to a value of
100% for wt mice treated with vehicle only.
TABLE-US-00039 TABLE 12 Total Pro-CNP38 received Naso- (nmol/kg)
Tail Anal Femur Tibia Humerus Ulna Group 2 620 104.8%** 106.0%**
105.3%** 100.8% 102.5% 101.7% Group 3 700 106.7%** 106.5%**
104.1%** 102.5%** 102.6%* 102.8%* Group 4 350 104.9%** 105.1%**
107.3%** 102.7%** 102.3%** 103.6%** **p < 0.01, *p < 0.05
While all dose regimens of Pro-Gly-CNP37 increased axial and
appendicular growth parameters measured, daily dosing of
Pro-Gly-CNP37 promoted appendicular growth (femur, tibia, humerus,
and ulna) at a lower total dose level (Group 4) compared to
regimens with less frequent dosing (Groups 2 and 3).
Example 13
Efficacy in Mouse Model of Mild Achondroplasia
The efficacy of CNP variants in enhancing growth and correcting
achondroplasia was tested in a mouse model of mild achondroplasia,
using a strain of transgenic mice expressing a human FGFR-3 gene
having a G380R mutation (FGFR3.sup.ach) (Wang et al., Proc. Natl.
Acad. Sci. USA, 96(8): 4455-4460 (1999); Naski et al., Development
USA, 125: 4977-4988 (1998); U.S. Pat. Nos. 6,265,632 and
6,136,040).
At 3 weeks of age, FGFR3.sup.ach mice and their wild-type
littermates were anesthetized to have lateral whole-body X-ray
images taken by Faxitron, and randomized by body weight into the
following treatment groups (n=8/group): (1) wild-type/vehicle, (2)
FGFR3.sup.ach/vehicle, (3) FGFR3.sup.ach/CNP37, and (4)
FGFR3.sup.ach/PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36). The mice
received once daily subcutaneous (s.c.) administration of
designated test article (vehicle or 200 nmol/kg CNP variant) for 5
weeks. Satellite groups (n=3) were used to confirm exposure of each
test article following a single subcutaneous administration on Day
1. Wild-type male FVB mice that received daily s.c. injection of
vehicle for 5 weeks were used as a control for normal growth.
X-ray measurements at baseline and at the end of the study were
performed to determine change in the head length, area of the
skull, and the external auditory meatus (EAM, the ear canal running
from the outer ear to the middle ear). Body weight and tail length
were measured at least once weekly, using a digital caliper to
measure tail length, and body (naso-anal) length was measured after
5 weeks of treatment. Bone (tibia, femur, ulna and humerus) lengths
were measured using a digital caliper at necropsy.
On Day 37, all mice were sacrificed by terminal anesthesia and
whole-animal photographs and X-ray images by Faxitron were taken.
Left and right tibia, femur, humerus, and ulna were collected and
measured using a digital caliper. The left portions of each bone
were processed for histology, and the right portions were
snap-frozen for archival. Samples obtained from the bones were used
to evaluate the effects of CNP variants on endochondral bone
growth.
Treatment of FGFR3.sup.ach mice with CNP37 by once daily s.c.
injection for 5 weeks resulted in significantly increased body
length (FIG. 51), tail length (FIG. 52), distal bone (ulna and
tibia) lengths (FIGS. 53A and B), and proximal bone (humerus and
femur) lengths (FIGS. 54A and B). Moreover, treatment with CNP37
increased the head length (FIG. 56), area of the external auditory
meatus (FIG. 57), and spine length (FIG. 58) via extension of the
vertebral bodies. Additionally, treatment with CNP37 corrected
rhizomelia (disproportion of the length of the proximal limbs) of
FGFR3.sup.ach mice, i.e., restored proportional growth of proximal
bones, as assessed by the femur:tibia ratio (FIG. 55). Table 13
summarizes the percentage change in tail length, body (naso-anal)
length, and bone (femur, tibia, humerus and ulna) lengths in
FGFR3.sup.ach mice injected s.c. once daily with 200 nmol/kg of
either CNP37 or PEO24-GANRR-CNP22(K4R) ("CNP27-PEO24") (SEQ ID NO:
36) for 5 weeks, relative to a value of 100% for FGFR3.sup.ach mice
treated with vehicle only.
TABLE-US-00040 TABLE 13 Naso- Tail Anal Femur Tibia Humerus Ulna
CNP37 115%** 109%** 112%** 107%** 105%* 106%** CNP27- 100% 99% 100%
102% 100% 101% PEO24 **p < 0.01, *p < 0.05
The results of these studies show that CNP37 can stimulate spinal
and long bone growth, help to correct rhizomelia by preferentially
increasing the length of the femur over the tibia, and help to
restore craniofacial proportions in FGFR3.sup.ach mice. These
results indicate that CNP37 and potentially other CNP variants may
be effective in correcting the symptoms of achondroplasia and
treating subjects having defects in bone growth or in need of
increased bone growth.
Example 14
Measurement of Biomarkers and Evaluation of Immunogenicity in
Wild-Type and Achondroplastic Mice
Measurement of Biomarkers After CNP Administration
The levels of bone growth biomarkers were measured in wild-type and
achondroplastic (FGFR3.sup.ach) mice.
Transgenic FVB FGFR3.sup.ach mice (a mouse model of mild
achondroplasia) were treated daily for 5 weeks by subcutaneous
injection with either vehicle (30 mM acetic acid/acetate buffer, 1%
benzyl alcohol, 10% sucrose, pH 4.0), CNP22, CNP37 or
PEO24-GANRR-CNP22(K4R) ("CNP27-PEO24") (SEQ ID NO: 36) at 200
nmol/kg for each CNP compound, as described above. Plasma and serum
were collected during the study. Harvested plasma was stored with
10.times. protease inhibitor at -80.degree. C. until analysis. cGMP
levels were measured 15 minutes post-injection on Day 36 from
K2-ETDA plasma collection tubes. Fragments of cleaved collagen type
II (cartilage-associated biomarkers), osteocalcin (bone-associated
biomarker), and IgG (relating to immunogenicity) were measured from
terminal bleed serum at the end of the study (Day 37). cGMP was
measured using a commercially available ELISA kit (Cayman Chemical
Co., Cat. No. 581021.1), cleaved collagen type II (Cartilaps) was
measured using a commercially available kit from Immuno-diagnostic
Systems (Cat. No. 3CAL4000), and osteocalcin was measured using a
commercially available kit from Biomedical Technologies Inc.
(Stoughton, Mass.).
FIG. 59 shows an increase in cGMP plasma levels in FGFR3.sup.ach
mice treated with CNP37 or PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36)
compared to vehicle. FIGS. 60 and 61 show that administration of
CNP37 resulted in the greatest elevation of serum levels of cleaved
collagen type II and osteocalcin. These results indicate that
administration of the CNP peptides, in particular CNP37, to
FGFR3.sup.ach mice led to increased levels of bone growth markers,
suggesting increased bone formation and growth in CNP
peptide-treated FGFR3.sup.ach mice.
For evaluation of biomarkers in wild-type mice, wild-type FVB mice
were treated daily for 5 weeks by subcutaneous injection with
either vehicle (30 mM acetic acid/acetate buffer, 1% benzyl
alcohol, 10% sucrose, pH 4.0), 200 nmol/kg G-CNP37, 200 nmol/kg
PEO12-GANRR-CNP22(K4R) ("CNP27-PEO12") (SEQ ID NO: 36), or 20
nmol/kg or 70 nmol/kg Pro-Gly-CNP37. Plasma and serum were
collected during the study. Harvested plasma was stored with
10.times. protease inhibitor at -80.degree. C. until analysis. cGMP
levels were measured 15 minutes post-injection on Day 36 from
K2-ETDA plasma collection tubes. The levels of cleaved collagen
type II, bone-specific alkaline phosphatase, and IgG (relating to
immunogenicity) were measured from terminal bleed serum at the end
of the study (Day 37). cGMP and cleaved collagen type II
(Cartilaps) were measured as described above. Bone-specific
alkaline phosphatase was measured using a commercially available
kit (Cusabio, Cat. No. CSB-E11914m).
Administration of G-CNP37 significantly increased (p<0.05) the
level of cGMP (FIG. 62) and particularly the level of fragments of
cleaved collagen type II (FIG. 63) in wild-type mice compared to
vehicle. The significantly higher level of collagen type II
fragments resulting from administration of G-CNP37 indicates
turnover of the cartilage matrix, suggesting that G-CNP37
stimulated new bone formation in growing bones in wild-type
mice.
Both doses of Pro-Gly-CNP37 ("Pro-CNP38"), 20 and 70 nmol/kg,
significantly increased (p<0.05) plasma cGMP level 15 minutes
after administration compared to wild-type mice treated with
vehicle (FIG. 64). Administration of the higher dose (70 nmol/kg)
of Pro-Gly-CNP37 also significantly increased (p<0.05) the level
of cleaved collagen type II compared to vehicle-treated mice (FIG.
65), suggesting that the higher dose of Pro-Gly-CNP37 stimulated
cartilage matrix turnover prior to new bone formation in wild-type
mice. Furthermore, administration of the higher dose (70 nmol/kg)
of Pro-Gly-CNP37 increased (p<0.05) the level of bone-specific
alkaline phosphatase compared to vehicle-treated mice (FIG. 66),
suggesting that the higher dose of Pro-Gly-CNP37 increased bone
remodeling in wild-type mice.
Evaluation of Immunogenicity of CNP Variants
Because the CNP variants are peptide derivatives, it is possible
that administration of the peptides may lead to an immunogenic
response in vivo. To assess whether an immune response occurred
after successive administrations of CNP variant, measurement of
serum antibody levels was performed.
An IgG assay was performed to assess whether an IgG immune response
was triggered by 5 week exposure of achondroplastic FGFR3.sup.ach
mice to CNP22, CNP37 or PEO24-GANRR-CNP22(K4R) ("CNP27-PEO24") (SEQ
ID NO: 36). IgG is the most predominant immunoglobulin in mouse and
human serum and is produced as part of the secondary immune
response to an antigen. The IgG response to administration of the
CNP peptides was determined as follows. 96-well plates were coated
with 100 ng/mL CNP22, CNP37 or PEO24-GANRR-CNP22(K4R) (SEQ ID NO:
36) in BupH PBS buffer (Pierce/Thermo, Cat. No. 28372, Rockford,
Ill.). After overnight incubation, plates were blocked with
Casein-PBS blocking buffer (Pierce/Thermo, Cat. No. 37528) for two
hours at room temperature with shaking at 300 rpm. After washing
with wash buffer (1.times.PBS with 0.05% Tween), diluted serum
samples from a terminal bleed (diluted 1:25) were added to the
plate. Positive and negative controls were also loaded onto the
plate. Positive control was 1:25 diluted serum (pooled from 6
individual FVB mice) with anti-CNP22 antibody added (Bachem rabbit
anti-CNP22 IgG, Cat. No. T-4222) at 1:1000 dilution. Negative
control was the diluted pooled serum. After two hours of
incubation, the plates were washed and secondary antibody diluted
in blocking buffer was added to the wells. For the mouse serum
samples, anti-mouse IgG Fc.gamma. (peroxidase-conjugated
affini-pure goat anti-mouse IgG, Fc.gamma. fragment, Cat. No.
115-035-071, Jackson Immunoresearch, West Grove, Pa.) was added at
a 1:10,000 dilution. For the positive and negative controls,
anti-rabbit IgG-HRP (Santa Cruz Biotechnology, Cat. No. SC-2004,
Santa Cruz, Calif.) was added to blocking buffer. After two hours
of incubation at room temperature with shaking at 300 rpm, plates
were washed with wash buffer. 100 uL of TMB (One-step TMB,
Pierce/Thermo, Cat. No. 34022) was added to all wells. Plates were
incubated at room temperature for 15 minutes with shaking at 300
rpm. Colorimetric reactions were stopped by the addition of 100 uL
of 2 N H.sub.2SO.sub.4. Plates were read at 450 nm (Spectramax,
Molecular Devices, Sunnyvale, Calif.) and data was analyzed using
SoftMax Pro software (Molecular Devices).
Serum samples from FGFR3.sup.ach mice treated with CNP22 or
PEO24-GANRR-CNP22(K4R) (SEQ ID NO: 36) indicated no positive IgG
immune response in any of the mice. Only one out of nine
CNP37-treated FGFR3.sup.ach mice showed a slightly positive IgG
response.
The immunogenic response of wild-type mice administered G-CNP37,
PEO12-GANRR-CNP22(K4R) (SEQ ID NO: 36) or Pro-Gly-CNP37, as
measured by an increase in serum IgG level, was also assessed by
examining terminal bleed serum samples as described above. None of
the wild-type mice treated with PEO12-GANRR-CNP22(K4R) (SEQ ID NO:
36) or Pro-Gly-CNP37 (20 or 70 nmol/kg) showed a positive IgG
immune response, and only one out of six wild-type mice
administered G-CNP37 showed a slightly positive IgG response.
Measurement of Biomarkers Under Different CNP Dosing Regimens
Wild-type FVB mice were treated daily for 9 weeks by subcutaneous
(s.c.) injection of vehicle (30 mM acetic acid/acetate buffer, 1%
benzyl alcohol, 10% sucrose, pH 4.0) or Pro-Gly-CNP37 ("Pro-CNP38")
under different dosing regimens. Group 1 included vehicle-treated
mice injected s.c. daily for 9 weeks followed by 1 week of no
treatment. Group 2 included mice treated with Pro-Gly-CNP37 (20
nmol/kg) once daily for 1 week, followed by three doses per week
for 8 weeks, followed by 1 week of no treatment. Group 3 contained
mice treated with Pro-Gly-CNP37 (20 nmol/kg) once daily in
alternate weeks (weeks 1, 3, 5, 7 and 9), with 1 week of no
treatment following each week of treatment. Group 4 contained mice
treated with Pro-Gly-CNP37 (5 nmol/kg) once daily for 9 weeks
followed by 1 week of no treatment. Finally, Group 5 included mice
treated with Pro-Gly-CNP37 (5 nmol/kg) once daily for 5 weeks
without any week of no treatment. Terminal bleed serum was
collected from the mice, and cleaved collagen type II, total
alkaline phosphatase and total antibody levels were measured
therefrom. Cleaved collagen type II levels were measured as
described above. Total alkaline phosphatase levels, predominantly
from the liver and bones, were measured with the assistance of a
veterinary diagnostic laboratory testing facility (Antech).
A total antibody assay was developed to evaluate potential immune
response. The platform used for the total antibody assay was an
electrochemiluminescent assay (ECLA). The ECLA platform utilizes a
biotin-labeled drug (here, biotin-Pro-Gly-CNP37) and a
ruthenium-labeled drug (here, Ru--Pro-Gly-CNP37). The
biotin-labeled drug binds to a streptavidin-coated plate that
contains electrodes, and the ruthenium-labeled drug functions as
the detection component of the assay, as ruthenium can be
electrochemically stimulated. A drug-specific antibody (here, a
CNP-specific antibody) binds to the biotin-labeled drug and the
ruthenium-labeled drug and "bridges" the two labeled drugs. Among
the advantages of the ECLA platform, any isotype of antibody (IgG,
IgM, etc.) can be detected, and the ECLA assay is
species-independent.
The total antibody assay was performed as follows. Pro-Gly-CNP37
was labeled with biotin in a 4:1 challenge ratio, and Pro-Gly-CNP37
was also labeled separately with ruthenium in a 10:1 challenge
ratio. Both separate labeling reactions were quenched by the
addition of glycine, and the samples from both reactions were
buffer-exchanged to PBS. Low and high QCs were prepared by using
commercially available anti-CNP22 antibody (Bachem rabbit
anti-CNP22 IgG, Cat. No. T-4222) added at low and high
concentrations to 5% diluted FVB mouse serum. Mouse serum samples
were diluted to 5% using assay diluent (5% BSA in PBS, MSD Cat. No.
R93AA-1). A working solution was prepared by adding biotin-labeled
Pro-Gly-CNP37 to assay diluent, then adding ruthenium-labeled
Pro-Gly-CNP37 to assay diluent, and then combining the two
solutions together. Twenty-five uL of low and high QCs were loaded
onto the plate as controls. Then 25 uL of sample was added to a
non-binding 96-well plate, followed by the addition of 50 uL of
working solution to all wells. Samples and QCs were incubated with
the working solution at room temperature for 2 hours with shaking
at 350 rpm. In the meantime, an MSD streptavidin plate (MSD Cat.
No. L13SA-1) was blocked with blocking buffer (MSD Cat. No.
R93AA-1) at room temperature for 2 hours with shaking at 350 rpm.
At the end of the two hour incubation, the MSD streptavidin plate
was washed, and then 50 uL of sample or QC was transferred to the
MSD plate. The plate was then incubated at room temperature for 1
hour with shaking at 350 rpm. At the end of the 1 hour incubation,
the MSD plate was washed and 150 uL of 2.times. read buffer (MSD
Cat. No. R92TC-2) was added to the plate. The plate was read using
an MSD PR400 machine.
All five groups of mice exhibited substantially comparable levels
of cleaved collagen type II (FIG. 67). Treatment of the mice in
Group 5 with 5 nmol/kg Pro-Gly-CNP37 once daily for 5 weeks
significantly increased (p<0.001) total alkaline phosphatase
level (FIG. 68), which is indicative of bone remodeling. Serum
samples from each of the four groups of mice treated with
Pro-Gly-CNP37 did not show a positive antibody response in the
total antibody assay.
Not to be bound by any theory, there are possible explanations as
to why the four groups of CNP-treated mice did not exhibit
statistically significant difference in cleaved collagen type II
level compared to vehicle-treated mice, and why only the
CNP-treated mice in Group 5 exhibited a statistically significant
increase in total alkaline phosphatase level compared to
vehicle-treated mice. It is possible that because the
vehicle-treated mice in the study were also growing, cleaved
collagen type II and alkaline phosphatase, which are biomarkers of
growth, were also produced in the vehicle-treated mice. In
addition, there was a one week period of no treatment for the mice
in each of Groups 1 to 4, which possibly diluted out any changes in
the cleaved collagen type II and total alkaline phosphatase levels
between the CNP-treated mice in Groups 1-3 and the vehicle-treated
mice. There was no period of no treatment for the CNP-treated mice
in Group 5, and those mice exhibited significantly (p<0.001)
greater total alkaline phosphatase level compared to
vehicle-treated mice.
Example 15
Dose Responses of CNP Variants in Mice
The effects of varying doses of CNP variants were assessed in wild
type FVB mice.
For the dose study, in two separate studies (S1 and S2), groups of
10 mice were administered Pro-Gly-wtCNP37 ("Pro-CNP38") at 20 and
70 nmol/kg subcutaneously once daily for 36 injections. Tail length
and body weight were measured over the course of treatment. Animals
were sacrificed at the end of the experiment and bone length
assessed.
Tail length of vehicle treated animals was approximately 8 cm at
day 36, whereas animals treated with 20 nmol/kg Pro-CNP38 exhibited
tail length of approximately 8.75 cm and 70 nmol/kg Pro-CNP38
increased tail length to approximately 9.5 cm. Administration of
Pro-CNP38 in either dose induces a significant (p<0.05) relative
increase in total body length compared to control treated animals,
demonstrating approximately a 130% increase in growth velocity at
20 nmol/kg and approximately a 160% increase in growth velocity at
70 nmol/kg Pro-CNP38 (FIG. 69).
Treatment with Pro-CNP 38 also significantly increased bone length
in most of the long bones assessed as well as total naso-anal (body
length) of the animal. Table 14 is representative of the relative %
increase in bone length in treated animals in comparison to vehicle
treated animals.
TABLE-US-00041 TABLE 14 Naso- Pro-CNP38 Tail Anal LV4-6 Femur Tibia
Humerus Ulna 20 nmol/kg 8%* 7%* 10%* 7%* 4%* 3%* 3% 70 nmol/kg 16%*
13%* 13%* 10%* 7%* 4%* 5%* Relative % increase, *p < 0.05 ANNOVA
(Dunnett's) v vehicle
Bone mineral density (BMD) and bone mineral content (BMC) were also
evaluated after administration of Pro-CNP38 at different doses.
Results (FIG. 70) showed that administration of Pro-CNP38 at 70
nmol/kg significantly decreased bone mineral density (FIG. 70A) and
increased bone mineral content (FIG. 70B), suggesting there is a
delay in bone mineralization in treated animals, but the
mineralization process itself is not adversely affected by
treatment with CNP.
There were no significant changes in organ weight between Pro-CNP38
or vehicle treated animals.
Bioanalytical Studies of Dose Response in Mice
Bioanalytical studies were carried out to measure markers of CNP
activity after in vivo administration of varying doses of
Pro-CNP38. Plasma cGMP levels, serum levels of collagen type II and
serum levels of alkaline phosphatase from in vivo samples were
analyzed. Wild type mice were administered 20 and 70 nmol/kg
Gly-wtCNP37 ("CNP38"), 20 and 70 nmol/kg Pro-Gly-wtCNP37
("Pro-CNP38") and 70 and 200 nmol/kg of GHKSEVAHRFK-wtCNP27
("HSA-CNP27") (SEQ ID NO: 144) subcutaneously once daily for 36
days. Plasma was collected 15 minutes after the last injection on
day 36 and mice were sacrified 24 hr later. At sacrifice, terminal
bleed serum was collected and used for biomarker analysis as
described previously.
FIG. 71 shows that 20 nmol/kg CNP38 and 70 nmol/kg HSA-CNP27
signficantly increased plasma cGMP (p<0.01), raising plasma cGMP
levels to approximately 300 pmol and 400 pmol, respectively.
Adminsitration of 70 nmol/kg CNP38 increased cGMP to approximately
500 pmol (p<0.01), while administration of 70 nmol/kg Pro-CNP38
increased cGMP to approximately 575 pmol (p<0.001).
Administration of 200 nmol/kg HSA-CNP27 increased cGMP to
approximately 675 pmol (p<0.001).
CNP variants also significantly increased serum levels of cleaved
collagen type II (FIG. 72). CNP38 at 20 nmol/kg increased collagen
to approximately 9 pg/ml (p<0.05), CNP38 at 70 nmol/kg increased
collagen to approximately 8 pg/ml (p<0.05), Pro-CNP38 at 20
nmol/kg increased collagen to approximately 12 pg/ml (p<0.05),
Pro-CNP38 at 70 nmol/kg increased collagen to approximately 16
pg/ml (p<0.05), HSA-CNP27 at 70 nmol/kg increased collagen to
approximately 10 pg/ml, and HSA-CNP27 at 200 nmol/kg increased
collagen to approximately 10 pg/ml (p<0.05).
Serum alkaline phosphatase (AP) levels also increased after
administration of CNP variants (FIG. 73). CNP38 at 20 nmol/kg
increased AP to approximately 130 IU/L, CNP38 at 70 nmol/kg
increased AP to approximately 160 IU/L (p<0.001), Pro-CNP38 at
20 nmol/kg increased AP to approximately 155 IU/L (p<0.001),
Pro-CNP38 at 70 nmol/kg increased AP to approximately 180 IU/L
(p<0.001), HSA-CNP27 at 70 nmol/kg increased AP to approximately
120 IU/L, and HSA-CNP27 at 200 nmol/kg increased AP to
approximately 140 IU/L (p<0.01). Table 15 illustrates the
percent of total AP that is bone specific.
TABLE-US-00042 TABLE 15 Total AP Bone-specific AP % of Total IU/L
IU/L Alk Phos Vehicle 109.8 24.7 22.5 CNP38 (20 nmol/kg) 135.9 47.9
35.2 CNP38 (70 nmol/kg) 166.3 63.5 38.2 Pro-CNP38 (20 nmol/kg)
159.9 65.9 41.2 Pro-CNP38 (70 nmol/kg) 183.6 78.4 42.7 HSA-CNP27
(20 nmol/kg) 121.4 51.7 42.6 HSA-CNP27 (70 nmol/kg) 136.1 76.5
56.2
Analysis of anti-CNP antibodies showed that only HSA-CNP27 elicited
an IgG antibody response in mice.
The results above illustrate that administration of CNP variants
increases the concentration of collagen type II and alkaline
phosphatase in serum, indicating that CNP increases factors
relevant for increased bone growth, and suggest that administration
of CNP variants at doses as low as 20 nmol/kg are effective in
increasing bone growth in vivo.
cGMP Response After Different Dose Regimens
Bioanalytical analysis was also assessed at different times after
administration of Pro-Gly-wtCNP37 ("Pro-CNP38") to wild type CD-1
mice, 8-10 weeks old, (n=3 per treatment group). Pro-CNP38 was
given in a single subcutaneous dose of 200 nmol/kg and levels of
cGMP measured at 15 minutes, 3 hours, 1 day, 2 days and 3 days post
injection in the plasma, epiphysis, cortical bone (marrow removed),
lung and brain. Blood was collected on K2EDTA. Tibial and femoral
epiphysis and cortical bone, ear pinna, brains, kidneys and lungs
were harvested, placed in boiling water for 5 minutes, then frozen
to -70.degree. C. Both plasma and tissue were assayed for cGMP
(Cayman Chemical cyclic GMP ELISA kit).
Results showed that cGMP levels increased at 15 minutes post
injection in plasma (approximately 1300 pmol/ml) and epiphysis
(approximately 2.5 pmol/ml/mg). By 3 hours, plasma levels had
decreased approximately to control levels, while levels in the
epiphysis were approximately 3 fold lower than cGMP levels at 15
minutes post injection, but higher than control levels. Levels of
cortical bone increased to approximately 0.5 pmol/ml/mg at 15
minutes and remained at this level at 3 hours. Levels of cGMP at
all timepoints were back to control levels by 1 day after
injection. Little to no cGMP was detected in lung or brain at any
timepoint.
cGMP levels were also measured in mice administered multiple
injections of Pro-CNP38. Groups of mice (n=3) were given Pro-CNP38
as follows: 20 nmol/kg single dose, subcutaneously; 200 nmol/kg
single dose, subcutaneously; 20 nmol/kg subcutaneously on days 0
and 1; 200 nmol/kg subcutaneously on days 0 and 1; 20 nmol/kg
subcutaneously on days 0 and 3; 200 nmol/kg subcutaneously on days
0 and 3. Mice were sacrificed 15 minutes after the final dose of
Pro-CNP38 and plasma levels of cGMP analyzed. There does not appear
to be a modulation of the plasma cGMP signal due to the different
dose regimens. cGMP responses in the cartilage are also
investigated.
Evaluation of Potential Desensitization of NPR-B Receptor
Histological analysis shows that CNP, when administered daily at
200 nmol/kg, accumulates in the growth plate of animals, based on
increased CNP immunoreactivity. It is possible that this
accumulation, or daily stimulation of the CNP receptor, in the
growth plate could desensitize the CNP receptor.
To determine if multiple dosing desensitizes the NPR-B receptor in
vitro, normal human articular chondrocytes were cultured with
Pro-Gly-wtCNP37 ("Pro-CNP38") for varying times and cGMP secretion
measured.
Primary normal human chondrocytes isolated from articular cartilage
were cultured as recommended by the supplier (Lonza). At 60-80%
confluence the chondrocytes were treated with 1 uM Pro-CNP38 twice,
with increasing amounts of time between treatments (first treatment
at time 0, then at 15 min, 30 min, 60 min, 2 hrs, 3 hrs, 4 hrs, 6
hrs after the initial treatment). In the following experiment the
treatments were either applied twice, with increasing amounts of
time between treatment (first treatment at time 0, then at 6, 16,
24, 48 hrs after initial treatment) or only once, in parallel to
the second treatment (only at 6, 16, 24 and 48 hrs-naive
responses). Treatments were either applied for 15 min only (acute
treatment), or for the duration of the experiment (chronic
treatment when CNP was left in the medium). Cell lysates and
conditioned medium were collected and analyzed for total cGMP
secretion (Molecular Devices ELISA).
In a short term experiment, cells were stimulated with Gly-wtCNP37
("CNP38") 1 uM two times for 15 minutes (acute treatment), or two
times with CNP38 throughout culture (chronic treatment). The
periods between treatments were 15 min, 30 min, 60 min, 2 hrs, 3
hrs, 4 hrs, and 6 hrs. Peak cGMP stimulation was obtained by
treating cells once only, at the last time point of the experiment,
(6 hours; >0.1 pmol/well in the acute experiment and 0.2
pmol/well in the chronic experiment). In the acute experiment, when
the cells are treated twice, the response decreases to 0.1
pmol/well if the cells are treated at time 0 and then again at 15
minutes. In the acute experiment, when the cells are treated twice,
the response decreases to approximately 0.5 pmol/well if the cells
are treated at time 0 and again at 30 min, 60 min, 2, 3, and 4
hours. In the chronic experiment, when the cells are treated twice,
the response decreases to approximately 0.16 pmol/well if the cells
are treated at time 0 and at 15 minutes. In the chronic experiment,
when the cells are treated twice, the response decreases to
approximately 0.6 pmol/well if the cells are treated at time 0 and
again at 30 min, 60 min, or 2 hours. In the chronic experiment,
when the cells are treated twice, the response decreases to <0.5
pmol/well if the cells are treated at time 0 and again at 2, 4 or 6
hours.
Long term studies were also carried out. For acute treatment, 1 uM
CNP38 was added to cell culture as described above. For chronic
treatment, 1 uM CNP38 was added to cell culture throughout the
experiment duration as described above. Results indicate that the
NPR-B receptor can desensitize after repeated, daily CNP
administration in vitro, and 48 hours between doses is sufficient
to recover to 60% of the maximial NPR-B response to CNP38, if the
CNP is removed after the first dose, and to <40% if the CNP is
incubated throughout the experiment (FIG. 74).
To evaluate whether treatment with a CNP variant desensitizes the
NPR-B receptor in vivo, experiments were conducted with wild-type
mice. CD-1 male mice 8-10 weeks of age were injected subcutaneously
with vehicle control or Pro-Gly-wtCNP37 ("Pro-CNP38") at 200
nmol/kg on the appropriate days. The mice were either injected with
Pro-CNP38 daily for up to 8 days, or were injected with Pro-CNP38
on the first day, the first and second days, or the first and third
days of the study. Fifteen minutes following the final injection,
the mice (n=3 per treatment group) were deeply anesthetized and
ex-sanguinated via thoracotomy and aortic cannulation. Circulating
blood was flushed from the body with PBS via an aortic cannula. The
kidneys and right tibias, right femurs and left femurs were
collected, boiled in water for 5 minutes and/or snap frozen in
liquid nitrogen and stored on dry ice or in a -70.degree. C.
freezer until cGMP assay. For estimation of cGMP production in
cartilage, distal femurs and/or proximal tibias were dissected,
weighed and pulverized using Covaris Cryoprep CP02. Powdered
samples were homogenized using a Covaris E210 sonicator in 5%
perchloric acid, and neutralized in 60% KOH. Samples were then
centrifuged at 10,000 rpm at 4.degree. C. for 5 minutes, and the
supernatant was used for the cGMP assay (Cyclic GMP Enzyme
Immunoassay Kit, Cayman, Mich.). Additionally, left tibias were
collected, fixed in 10% normal buffered formalin, and saved for
further immunohistochemical analysis.
FIG. 75A shows that repeated daily treatment of wild-type mice with
200 nmol/kg of Pro-CNP38 for 1, 4, 6, 7 and 8 days did not result
in desensitization of the cGMP response. To the contrary,
potentiation of the cGMP response was observed after 4 days of
daily treatment. Further daily treatment resulted in a plateau of
the cGMP reponse, up to 8 days. The results indicate that daily
treatment of wild-type mice with 200 nmol/kg of Pro-CNP38 for up to
8 days do not desensitize the cGMP response.
The kinetics of the cGMP response in distal femoral cartilage after
treatment of the wild-type mice with Pro-CNP38 was also
investigated. Treatment of the mice once a day for two days
potentiated the cGMP response to Pro-CNP38, compared with the cGMP
response to a single treatment (FIG. 75B). When the mice were
treated on the first and third days, but not on the first and
second days, the cGMP response after the second treatment (on the
third day) was substantially similar to the cGMP response observed
after a single treatment on day one (FIG. 75B). The results suggest
that dosing on consecutive days is beneficial for potentiating the
cGMP response to Pro-CNP38 in this mouse study.
Activation of NPR-B in Different Tissues
To evaluate potential activation of NPR-B in different tissues by a
CNP variant, wild-type male CD-1 mice were injected with 200
nmol/kg of Gly-CNP37, and the cGMP response was measured in certain
tissues at different time points. Two mice were used for each
treatment group. Each mouse received one subcutaneous injection of
Gly-CNP37 or vehicle control. Fifteen, 30 or 60 minutes or 3 hours
following the injection, the mice were deeply anesthetized and
ex-sanguinated via thoracotomy and aortic cannulation. Circulating
blood was removed by a PBS flush with an aortic cannula. Heart,
liver, lung, kidney, ear pinna, aorta and brain were collected. All
tissues were boiled in water for 5 minutes, finely dissected
(including flushing of marrow from femoral cortices with PBS),
weighed, cooled in liquid nitrogen, and pulverized in a
BioPulverizer. The resulting powdered samples were homogenized with
a Polytron in 6% pre-chilled perchloric acid and neutralized with
60% KOH. Samples were then centrifuged at 10,000 rpm and 4.degree.
C. for 5 minutes, and the supernatant was used for the assay of
cGMP (Cyclic GMP Enzyme Immunoassay Kit, Cayman Chemical Company,
Ann Arbor, Mich.). The results were normalized for tissue
weight.
Secretion of cGMP in response to treatment with Gly-CNP37 ("CNP" in
FIG. 76) was detectable in distal femurs (cartilage and bone),
femoral cortices (bone), ear pinna (cartilage), and kidney (FIGS.
76A-D). Maximal cGMP responses in those tissues were observed 15
min after treatment. Liver, heart, lung and brain tissues did not
exhibit appreciable cGMP secretion in response to Gly-CNP37
relative to vehicle control at the studied time points (FIGS.
76E-H). The results indicate that treatment with 200 nmol/kg of
Gly-CNP37 stimulated cGMP secretion in cartilage, bone and renal
tissues.
Example 16
Dose Responses of CNP Variants in Monkeys
The effects of the CNP variant Pro-Gly-CNP37 on bone growth and the
levels of bone growth-related biomarkers are evaluated in
cynomolgus monkeys. Eight normal juvenile cynomolgus monkeys (about
2.5 years of age at the start of the on-going study) are
subcutaneously injected daily with 10 or 36 .mu.g/kg/day of
Pro-Gly-CNP37 (n=4 per dose group). Four such monkeys are
administered vehicle as control. The total length of treatment is 6
months. Various measures of growth plate expansion and bone growth
are made by digital X-ray and magnetic resonance imaging, and by
measurement of limb and body lengths externally. Blood and urine
samples are collected periodically for clinical pathology and
measurement of levels of Pro-Gly-CNP37 and biomarkers. Following
termination of the study, gross pathology is performed and tissue
samples are evaluated histologically for assessment of efficacy and
safety.
Data obtained thus far in the on-going study show that both doses
of Pro-Gly-CNP37 have increased growth plate width by digital X-ray
(FIG. 77), increased right and left tibia lengths by digital X-ray
(FIGS. 78A and B), increased leg length by external measurement
(FIG. 79), increased arm length by external measurement (FIG. 80),
increased body length by external measurement (FIG. 81), and
increased the serum level of alkaline phosphatase, a biomarker for
bone formation (FIG. 82). The data demonstrate that Pro-Gly-CNP37
can stimulate bone growth in normal juvenile cynomolgus monkeys at
hemodynamically acceptable doses.
Example 17
Effects of CNP Variants on Cardiovascular System in Mice
Natriuretic peptides such as CNP have been reported to affect the
cardiovascular system. Wang et al. (Eur J Heart Fail. 9:548-57.
2007) describe that CNP has been shown to have a cardioprotective
effect in preventing myocardial ischaemia/reperfusion injury and
improving cardiac remodelling after myocardial infarction in rats.
Wang demonstrated that mice overexpressing CNP have reduced
incidence of cardiac hypertrophy caused by myocardial infarction.
Additionally, CNP has been shown to cause endothelium-independent
vasodilation (M. Honing et al., Hypertension, 37:1179-1183 (2001))
and therefore may transiently decrease blood pressure in vivo.
To assess the effects of CNP variants on the cardiovascular system,
the blood pressure and heart rate in anesthetized wild-type FVB
mice is studied following subcutaneous injection of the
variants.
After a pilot study to define a broad dose range of cardiovascular
activity, a dose-response study is conducted to examine the effects
of three different dose levels of each CNP variant. Three male FVB
mice aged 8 weeks comprise each treatment group. Doses are
administered subcutaneously to anesthetized mice, and systolic,
diastolic and mean arterial pressure (MAP), as well as heart rate,
are monitored via implanted intraarterial pressure transducers.
Example 18
Formulation of CNP Variants
CNP preformulation studies were carried out to assess the stability
of CNP variant Gly-wtCNP37 ("CNP38") at different pH's (pH 3, 4, 5,
6, 7 and 8) and temperatures (5.degree. C., 25.degree. C., and
40.degree. C.) overtime. CNP38 exhibited greater stability at pH
4-6 than at the other pH's in the studies. CNP38 was stable at
5.degree. C. at pH 4-6, with .gtoreq.about 95% of CNP38 remaining
after 15 weeks. When the temperature was raised to 25.degree. C.,
at pH 4 about 85% of CNP38 remained after 15 weeks, at pH 5 about
85% remained after 15 weeks, and at pH 6 about 80% remained after
15 weeks. When the temperature was raised to 40.degree. C., at pH 4
about 55-60% of CNP38 remained after 15 weeks, at pH 5 about 65%
remained after 15 weeks, and at pH 6 about 40% remained after 15
weeks. FIG. 83 illustrates the observed plot of pseudo-first order
degradation rate constant (K.sub.obs) vs. pH from pH 3 to 8 and at
5.degree. C., 25.degree. C. and 40.degree. C. The stability data
for CNP38 in the preformulation studies suggests CNP formulations
having a pH in the range from about 4 to about 6. An acidic pH
(e.g., pH.ltoreq.about 6) can promote the stability of a CNP
variant by, e.g., minimizing or avoiding deamidation of asparagine
and/or glutamine residue(s), isomerization of aspartic acid
residue(s), or degradation of the CNP variant by other
pathways.
CNP variants can be formulated in pharmaceutical carriers for
administration to subjects affected by, e.g., bone growth
conditions. In some embodiments, liquid formulations of CNP
variants are formulated according to any combinations of the
ingredients and their amounts or concentrations in Table 16.
TABLE-US-00043 TABLE 16 Ingredient Class Ingredient Concentration
Range Active ingredient CNP variant 10 mg/mL .+-. 9.9 mg/mL
Buffering agent Acetic acid/acetate 10 mM .+-. 5 mM, or pH 5 .+-. 1
Buffering agent Citric acid/citrate 10 mM .+-. 5 mM, or pH 5 .+-. 1
Isotonicity-adjusting NaCl 140 mM .+-. 20 mM agent
Isotonicity-adjusting Sucrose 10% .+-. 5% agent Preservative
m-Cresol 0.4% .+-. 0.1% or 0.2% Preservative/anti- Benzyl alcohol
1.5% .+-. 0.5% adsorbent Stabilizer Glycerin (glycerol) 5%-100%
(neat).sup.1 Stabilizer Methionine 0.01%-0.2% Stabilizer Ascorbic
acid/ 0.1%-l% ascorbate salt Stabilizer Thioglycerol 0.1%-l%
Anti-adsorbent Polysorbate 20 0.001%-0.5% Polysorbate 80
0.001%-0.5% Benzyl alcohol 0.5%-1.5% .sup.1Glycerin is used to
minimize or prevent water-driven hydrolysis, deamidation,
isomerization or cleavage of CNP variants. For lyophilized
formulations, 4-6% or 6-20% mannitol or sucrose can be substituted
for NaCl.
In certain embodiments, lyophilized formulations of CNP variants
are prepared from formulations formulated according to any
combinations of the ingredients and their amounts or concentrations
in Table 17.
TABLE-US-00044 TABLE 17 Ingredient Class Ingredient Concentration
Range Active ingredient CNP variant 10 mg/mL .+-. 9.9 mg/mL
Buffering agent Acetic acid/acetate 10 mM .+-. 5 mM, or pH 5 .+-. 1
Buffering agent Citric acid/citrate 10 mM .+-. 5 mM, or pH 5 +.+-.
1 Isotonicity-adjusting Sorbitol 5% .+-. 3% agent/bulking agent
Isotonicity-adjusting Mannitol 5% .+-. 3% agent/bulking agent
Isotonicity-adjusting Sucrose 10% .+-. 5% agent/bulking agent
Preservative m-Cresol 0.4% .+-. 0.2% Preservative/anti- Benzyl
alcohol 1.5% .+-. 0.5% adsorbent Stabilizer Glycerin (glycerol)
5%-100% (neat).sup.1 Stabilizer Methionine 0.01%-0.2% Stabilizer
Ascorbic acid/ 0.1%-l% ascorbate salt Stabilizer Thioglycerol
0.1%-l% Anti-adsorbent Polysorbate 20 0.001%-0.5% Polysorbate 80
0.001%-0.5% Benzyl alcohol 0.5%-1.5% .sup.1Glycerin is used to
minimize or prevent water-driven hydrolysis, deamidation,
isomerization or cleavage of CNP variants.
In certain embodiments, a formulation comprising a CNP variant has
a pH of about 3-7, or about 3-6, or about 3.5-6.5, or about 4-6, or
about 4-5, or about 4.5-5.5. In some embodiments, for pH 4-5.5 a
suitable buffering agent is acetic acid/acetate (e.g., sodium
acetate), and for pH 5.5-6 a suitable buffering agent is citric
acid/citrate. Citric acid/citrate (e.g., sodium citrate) is also a
suitable buffering agent in the range of pH 3-6 or pH 4-6. In
certain embodiments, the buffering agent has a concentration in the
formulation of about 2-50 mM, or about 2-40 mM, or about 2-30 mM,
or about 5-30 mM, or about 2-20 mM, or about 5-20 mM, or about 5-15
mM.
To minimize or avoid deamidation of a CNP variant, the variant can
be formulated in pharmaceutically acceptable organic cosolvents,
such as glycerin, ethanol, and propylene glycol. Because
deamidation occurs by hydrolysis, substitution of an organic
cosolvent for water minimizes contact of the CNP variant with
water. The concentration of one or more organic solvents in an
organic-aqueous solvent system can be, e.g., from about 10% to
about 99%, or about 100% if water is not used.
Also to minimize or avoid deamidation of a CNP variant, water can
be removed from the formulation by lyophilization. In some
embodiments, lyophilized formulations contain any combinations of
the following components: buffer: sodium acetate and acetic acid,
or sodium citrate and citric acid; isotonicity/bulking agent:
mannitol (e.g., 3-10%, 2-8% or 4-6%); sucrose (e.g., 6-20%, 5-15%
or 8-12%); antioxidants: methionine and/or ascorbic acid with molal
ratio of each antioxidant to CNP variant from about 0.1:1 to about
1:1, or from about 0.5:1 to about 5:1, or from about 1:1 to about
15:1, or from about 1:1 to about 10:1, or from about 3:1 to about
10:1.
Deamidation can also be minimized or avoided by storing a CNP
composition (e.g., a liquid formulation or a lyophilized
formulation) at lower temperature, such as at about 5.degree. C.,
0.degree. C., -10.degree. C., -20.degree. C., -30.degree. C.,
-40.degree. C., -50.degree. C., -60.degree. C., -70.degree. C.,
-80.degree. C., -90.degree. C., or -100.degree. C.
To minimize or avoid oxidation of oxidizable residues (e.g.,
methionine) in a CNP variant, the variant can be formulated with
one or more antioxidants. Exemplary antioxidants include, but are
not limited to, methionine, ascorbic acid, and thioglycerol.
Oxidation of, e.g., methionine residues can also be minimized or
prevented by purging oxygen from a liquid medium (if a liquid
formulation) with nitrogen or argon, and/or by purging oxygen from
a container or packaging with nitrogen or argon.
In some embodiments, to minimize or prevent adsorption (e.g.,
adsorption of a CNP variant to plastic or glass), Polysorbate 20,
Polysorbate 80 or benzyl alcohol, or a combination thereof, is
added to a CNP formulation. In certain embodiments, each of the
anti-adsorbent(s) is in a concentration from about 0.001% to about
0.5%, or from about 0.01% to about 0.5%, or from about 0.1% to
about 1%, or from about 0.5% to about 1%, or from about 0.5% to
about 1.5%, or from about 0.5% to about 2%, or from about 1% to
about 2%. Exemplary range(s) of anti-adsorbent(s) in the
formulation include without limitation from about 0.001% to about
0.5% of Polysorbate 20, from about 0.001% to about 0.5% of
Polysorbate 80, and/or from about 0.5% to about 1.5% of benzyl
alcohol.
In certain embodiments, a liquid CNP formulation comprises, or a
lyophilized CNP formulation is prepared from a formulation that
comprises, (1) an acetic acid/acetate (e.g., sodium acetate) buffer
having a concentration of about 30 mM.+-.5 or 10 mM buffering agent
and a pH of about 4.+-.0.5 or 1, and (2) benzyl alcohol (e.g., as a
preservative and/or anti-adsorbent) at a concentration of about
1%.+-.0.5%, and optionally (3) sucrose at a concentration of about
10%.+-.5%.
Example 19
Clinical Evaluation of CNP Variants
The following example provides guidance on the parameters to be
used for the clinical evaluation of compositions comprising CNP22
or variants thereof in the therapeutic methods of the present
disclosure. As discussed herein, CNP22 or variants thereof will be
used in the treatment of disorders responsive to CNP, including
disorders of the bone and vascular smooth muscle. Clinical trials
will be conducted which will provide an assessment of doses of
CNP22 or variants thereof for safety, pharmacokinetics, and initial
response of both surrogate and defined clinical endpoints. The
trial will be conducted for a minimum, but not necessarily limited
to, 24 weeks to collect sufficient safety information on about 100
evaluable patients. The initial dose for the trials will vary from
about 0.001 to about 1.0 mg/kg/week, or any of the doses described
herein. In the event that the initial dose in this range does not
produce a significant direct clinical benefit, the dose should be
increased within this range or beyond this range as necessary, and
maintained for an additional minimal period of, but not necessarily
limited to, 24 weeks to establish safety and to evaluate efficacy
further.
Measurements of safety will include adverse events, allergic
reactions, complete clinical chemistry panel (including kidney and
liver functions), urinalysis, and CBC with differential. In
addition, other parameters relevant to clinical benefit will be
monitored. The present example also includes the determination of
pharmacokinetic parameters of CNP22 or variants thereof, including
absorption, distribution, metabolism, excretion, and half-life and
bioavailability in the blood. It is anticipated that such analyses
will help relate dose to clinical response.
Methods
Patients will undergo a baseline medical history and physical exam,
and a standard set of clinical laboratory tests (including CBC,
Panel 20, CH50, and UA). The patients will be followed closely with
weekly visits to the clinic. The patients will return to the clinic
for a complete evaluation one week after completing the treatment
period. Should dose escalation be required, the patients will
follow the same schedule outlined above. Safety will be monitored
throughout the trial.
Diagnosis and Inclusion Criteria
The patients may be male or female, with a documented diagnosis of
a potentially CNP-responsive disorder. A specific example of a
potentially CNP-responsive, bone-related disorder is
achondroplasia, which may be confirmed by genetic testing and other
evidence of an FGFR-3 mutation or dysfunction. The ideal age range
of achondroplasia patients will be from infant (<1 year of age)
to pre-adolescent (<13 years of age). A patient will be excluded
from this study if the patient is pregnant or lactating; has
received an investigational drug within 30 days prior to study
enrollment; or has a medical condition, serious intercurrent
illness, or other extenuating circumstance that may significantly
decrease study compliance.
Safety
Therapy with CNP22 or variants thereof will be determined to be
safe if no significant acute or chronic drug reactions occur during
the course of the study. The longer-term administration of the drug
will be determined to be safe if no significant abnormalities are
observed in the clinical examinations, clinical labs, or other
appropriate studies.
It has been shown that compared to wild-type CNP22, certain CNP
variants of the disclosure are much more resistant to NEP
degradation in vitro, have a much longer plasma half-life and
bioavailability in rats, stimulate a much higher level of cGMP
production in rats, and/or induce a significantly greater increase
in long bone length and body length in achondroplastic mice.
Furthermore, it has been shown that short duration dose regimen
treatments with CNP22 are nearly as effective as continuous CNP22
treatment in reversing FGF2-induced arrest of chondrocyte growth in
vitro. These results, among others described herein, demonstrate
the utility of CNP variants of the disclosure in treating
CNP-responsive conditions or disorders such as, e.g., bone-related
disorders and vascular smooth muscle disorders.
It is understood that every embodiment of the disclosure described
herein may optionally be combined with any one or more of the other
embodiments described herein. Every patent literature and every
non-patent literature cited herein are incorporated herein by
reference in their entirety.
Numerous modifications and variations to the disclosure, as set
forth in the embodiments and illustrative examples described
herein, are expected to occur to those skilled in the art.
Consequently only such limitations as appear in the appended claims
should be placed on the disclosure.
SEQUENCE LISTINGS
1
237122PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREwtCNP-22DISULFID(6)..(22)Cysteines in
positions 6 and 22 form cyclic domain via disulfide bond 1Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15
Met Ser Gly Leu Gly Cys 20 217PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURECNP-17 2Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser Met Ser Gly Leu Gly 1 5 10 15 Cys 327PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATURECNP-27 3Gly Ala Asn Lys
Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg
Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 453PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATURECNP-53 4Asp Leu Arg Val
Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu 1 5 10 15 Gln Glu
His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly 20 25 30
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 35
40 45 Ser Gly Leu Gly Cys 50 522PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Gly is optionally modified with an amino
acid sequence derived from a natriuretic polypeptide, a
non-natriuretic polypeptideMOD_RES(22)..(22)Cys is optionally
modified with an amino acid sequence derived from a natriuretic
polypeptide, a non-natriuretic polypeptide 5Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 622PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Gly is modified with a synthetic or
natural polymeric group or combination thereofMOD_RES(4)..(4)Xaa is
Lys or a conservative amino acid substitution thereof, any natural
or unnatural amino acid or peptidomimetic that does not have a
reactive primary amine on a side chain, Arg, Gly,
6-hydroxy-norleucine, citrulline (Cit), Gln, Glu or
SerMOD_RES(10)..(10)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid or
peptidomimetic that does not have a reactive primary amine on a
side chain, Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln,
Glu or SerMOD_RES(22)..(22)Cys is optionally modified with a
synthetic or natural polymeric group or combination thereof 6Gly
Leu Ser Xaa Gly Cys Phe Gly Leu Xaa Leu Asp Arg Ile Gly Ser 1 5 10
15 Met Ser Gly Leu Gly Cys 20 75PRTArtificial SequenceSynthetic
Polypeptide 7Gly Ala Asn Lys Lys 1 5 85PRTArtificial
SequenceSynthetic Polypeptide 8Gly Ala Asn Arg Arg 1 5
95PRTArtificial SequenceSynthetic Polypeptide 9Gly Ala Asn Pro Arg
1 5 105PRTArtificial SequenceSynthetic Polypeptide 10Gly Ala Asn
Gln Gln 1 5 115PRTArtificial SequenceSynthetic Polypeptide 11Gly
Ala Asn Ser Ser 1 5 125PRTArtificial SequenceSynthetic Polypeptide
12Gly Ala Asn Arg Gln 1 5 135PRTArtificial SequenceSynthetic
Polypeptide 13Gly Ala Asn Arg Met 1 5 145PRTArtificial
SequenceSynthetic Polypeptide 14Gly Ala Asn Arg Thr 1 5
155PRTArtificial SequenceSynthetic Polypeptide 15Gly Ala Asn Arg
Ser 1 5 1613PRTArtificial SequenceSynthetic Polypeptide 16Ala Ala
Trp Ala Arg Leu Leu Gln Glu His Pro Asn Ala 1 5 10
1714PRTArtificial SequenceSynthetic Polypeptide 17Ala Ala Trp Ala
Arg Leu Leu Gln Glu His Pro Asn Ala Arg 1 5 10 1814PRTArtificial
SequenceSynthetic Polypeptide 18Asp Leu Arg Val Asp Thr Lys Ser Arg
Ala Ala Trp Ala Arg 1 5 10 1915PRTArtificial SequenceSynthetic
Polypeptide 19Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn
Lys Lys 1 5 10 15 2015PRTArtificial SequenceSynthetic Polypeptide
20Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Arg Arg 1 5
10 15 2131PRTArtificial SequenceSynthetic Polypeptide 21Asp Leu Arg
Val Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu 1 5 10 15 Gln
Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys 20 25 30
2231PRTArtificial SequenceSynthetic Polypeptide 22Asp Leu Arg Val
Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu 1 5 10 15 Gln Glu
His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Arg Arg 20 25 30
236PRTArtificial SequenceSynthetic Polypeptide 23Ser Leu Arg Arg
Ser Ser 1 5 245PRTArtificial SequenceSynthetic Polypeptide 24Asn
Ser Phe Arg Tyr 1 5 259PRTArtificial SequenceSynthetic Polypeptide
25Ser Pro Lys Met Val Gln Gly Ser Gly 1 5 266PRTArtificial
SequenceSynthetic Polypeptide 26Met Val Gln Gly Ser Gly 1 5
276PRTArtificial SequenceSynthetic Polypeptide 27Lys Val Leu Arg
Arg Tyr 1 5 286PRTArtificial SequenceSynthetic Polypeptide 28Lys
Val Leu Arg Arg His 1 5 2912PRTArtificial SequenceSynthetic
Polypeptide 29Gly Gln His Lys Asp Asp Asn Pro Asn Leu Pro Arg 1 5
10 309PRTArtificial SequenceSynthetic Polypeptide 30Gly Val Pro Gln
Val Ser Thr Ser Thr 1 5 3115PRTArtificial SequenceSynthetic
Polypeptide 31Gly Glu Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu
Ser Gln 1 5 10 15 3214PRTArtificial SequenceSynthetic Polypeptide
32Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 1 5 10
3315PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAmino
acid extention 33Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala
Asn Lys Lys 1 5 10 15 3437PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURECNP-22 with amino acid
extentionMISC_FEATURE(19)..(19)Xaa is Lys or a conservative amino
acid substitution thereof, any natural or unnatural amino acid,
Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Glu or
SerMISC_FEATURE(25)..(25)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid, Arg,
Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Glu or
SerMISC_FEATURE(37)..(37)Cys is optionally modified with a
synthetic, natural or combination polymeric group 34Gln Glu His Pro
Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly 1 5 10 15 Leu Ser
Xaa Gly Cys Phe Gly Leu Xaa Leu Asp Arg Ile Gly Ser Met 20 25 30
Ser Gly Leu Gly Cys 35 3522PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURECNP22 (K4R) 35Gly Leu Ser Arg Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly
Cys 20 3627PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANRR-CNP22(K4R) - CNP27(Arg4) 36Gly Ala Asn
Arg Arg Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp
Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 3727PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREGANPR-CNP22(K4R) -
CNP27(Pro4) - Analog CI 37Gly Ala Asn Pro Arg Gly Leu Ser Arg Gly
Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser Met Ser Gly
Leu Gly Cys 20 25 3824PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREER-CNP22 38Glu Arg Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile 1 5 10 15 Gly Ser Met Ser Gly Leu
Gly Cys 20 3924PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BA - ER-CNP22(K4R) 39Glu Arg Gly Leu
Ser Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile 1 5 10 15 Gly Ser
Met Ser Gly Leu Gly Cys 20 4023PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURER-CNP22 40Arg Gly Leu Ser Lys Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly 1 5 10 15 Ser Met Ser Gly Leu Gly
Cys 20 4123PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AZ - R-CNP22(K4R) 41Arg Gly Leu Ser
Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 1 5 10 15 Ser Met
Ser Gly Leu Gly Cys 20 4222PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURECNP22 (K4R)(K10R) 42Gly Leu Ser Arg Gly Cys
Phe Gly Leu Arg Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 4322PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATURECNP-22 (Cys6-Phe7 variable
bond)MOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-) replaced
with -CH2-NH-MOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-)
replaced with -C(=O)-N(R)- where R is methyl, ethyl, n-propyl,
isopropyl, cyclopropyl, n-butyl, isobutyl, sec-butyl or
tert-butylMOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-)
replaced with -C(=O)-NH-CH2-MOD_RES(6)..(7)Cys6-Phe7 peptide bond
(-C(=O)-NH-) replaced with -CH2-S-MOD_RES(6)..(7)Cys6-Phe7 peptide
bond (-C(=O)-NH-) replaced with -CH2-S(O)n where n is 1 or
2MOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-) replaced with
-CH2-CH2-MOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-)
replaced with -CH=CH-MOD_RES(6)..(7)Cys6-Phe7 peptide bond
(-C(=O)-NH-) replaced with -C(=O)-CH2-MOD_RES(6)..(7)Cys6-Phe7
peptide bond (-C(=O)-NH-) replaced with
-CH(CN)-NH-MOD_RES(6)..(7)Cys6-Phe7 peptide bond (-C(=O)-NH-)
replaced with -CH(OH)-CH2-MOD_RES(6)..(7)Cys6-Phe7 peptide bond
(-C(=O)-NH-) replaced with -O-C(=O)-NH-MOD_RES(6)..(7)Cys6-Phe7
peptide bond (-C(=O)-NH-) replaced with NHC(=O)NH- 43Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Gly Cys 20 4422PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(7)..(7)Xaa is 3-amino-2-phenylpropionic acid or
D-Phe 44Gly Leu Ser Lys Gly Cys Xaa Gly Leu Lys Leu Asp Arg Ile Gly
Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 4522PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATURECNP22 - modified
Cys6MOD_RES(6)..(6)Xaa is Cys, homocysteine, penicillamine,
2-mercaptopropionic acid, or 3-mercaptopropionic acid. 45Gly Leu
Ser Lys Gly Xaa Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15
Met Ser Gly Leu Gly Cys 20 4622PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Gly is optionally modified with a bone or
cartilage targeting compound, a biphosphonate, polyAsp, polyGlu,
osteopontin, osteocalcin, or sialoprotein peptides, polymers, PEG,
carbohydrates, hydrophobic acids, or amino acidsMOD_RES(4)..(4)Xaa
is Lys or a conservative amino acid substitution thereof, any
natural or unnatural amino acid or peptidomimetic that does not
have a reactive primary amine on a side chain, Arg, Gly,
6-hydroxy-norleucine, citrulline (Cit), Gln, Ser, or
GluMOD_RES(6)..(7)Xaa is Cys with peptide bond isosteres between
positions 6 and 7MOD_RES(7)..(7)Xaa is L-Phe, D-Phe,
3-amino-2-phenylpropionic acid, or N-alkylated derivatives of
PheMOD_RES(8)..(8)Xaa is Gly, tert-butyl-Gly (tBu-Gly), Thr, Ser,
Val or AsnMOD_RES(9)..(9)Xaa is Leu, Ser, Thr or peptide bond
isosteres thereofMOD_RES(10)..(10)Xaa is Lys or a conservative
amino acid substitution thereof, any natural or unnatural amino
acid, Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
GluMOD_RES(11)..(11)Xaa is Leu or peptide bond isosteres
thereofMOD_RES(14)..(14)Xaa is Ile, tBu-Gly and peptide bond
isosteres thereofMOD_RES(17)..(17)Xaa is Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) or 2-amino-isobutyric acid
(Aib)MOD_RES(20)..(20)Xaa is Leu, norleucine (Nle), homoleucine
(Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg or peptide
bond isosteres thereofMOD_RES(22)..(22)Cys is optionally modified
with a bone or cartilage targeting compound, a bisphosphonate,
polyAsp, polyGlu, osteopontin, osteocalcin or sialoprotein
peptides, or amino acid sequences derived from non-CNP polypeptides
46Gly Leu Ser Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Asp Arg Xaa Gly Ser 1
5 10 15 Xaa Ser Gly Xaa Gly Cys 20 4722PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Gly is optionally
modified with a peptide sequence containing one to five amino acids
from a natriuretic or non-natriuretic polypeptideMOD_RES(1)..(1)Gly
is optionally modified with a peptide sequence containing one to
five amino acids from a natriuretic or non-natriuretic
polypeptideMOD_RES(4)..(4)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid or
peptidomimetic, Arg, 6-hydroxy-norleucine, citrulline (Cit), Gln,
Ser, or GluMOD_RES(6)..(6)Xaa is Cys or decarboxy
CysMOD_RES(6)..(7)Peptide bond between positions 6 and 7 may be
replaced with -CH2-NH-MOD_RES(6)..(7)Peptide bond between positions
6 and 7 may be replaced with -C(=O)-N(R)- where R is methyl, ethyl,
n-propyl, isopropyl, cyclopropyl, n-butyl, isobutyl, sec-butyl or
tert-butylMOD_RES(6)..(7)Peptide bond between positions 6 and 7 may
be replaced with -C(=O)-NH-CH2-MOD_RES(6)..(7)Peptide bond between
positions 6 and 7 may be replaced with
-CH2-S-MOD_RES(6)..(7)Peptide bond between positions 6 and 7 may be
replaced with -CH2-S(O)n where n is 1 or 2MOD_RES(6)..(7)Peptide
bond between positions 6 and 7 may be replaced with
-CH2-CH2-MOD_RES(6)..(7)Peptide bond between positions 6 and 7 may
be replaced with -CH=CH-MOD_RES(6)..(7)Peptide bond between
positions 6 and 7 may be replaced with
-C(=O)-CH2-MOD_RES(6)..(7)Peptide bond between positions 6 and 7
may be replaced with -CH(CN)-NH-MOD_RES(6)..(7)Peptide bond between
positions 6 and 7 may be replaced with
-CH(OH)-CH2-MOD_RES(6)..(7)Peptide bond between positions 6 and 7
may be replaced with -O-C(=O)-NH-MOD_RES(6)..(7)Peptide bond
between positions 6 and 7 may be replaced with
-NHC(=O)NH-MOD_RES(7)..(7)Xaa is L-Phe, D-Phe,
3-amino-2-phenylpropionic acid, or N-alkylated derivatives of
PheMOD_RES(8)..(8)Xaa is Gly, tert-butyl-Gly (tBu-Gly), Val, Ser,
Thr or AsnMOD_RES(9)..(9)Xaa is Leu, Ser, Thr or peptide bond
isosteres thereofMOD_RES(10)..(10)Xaa is Arg, Gly,
6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
GluMOD_RES(11)..(11)Xaa is Leu or peptide isosteres
thereofMOD_RES(14)..(14)Xaa is Ile, tBu-Gly or peptide isosteres
thereofMOD_RES(17)..(17)Xaa is Met, Val, Asn, beta-Cl-Ala,
2-aminobutyric acid (Abu) or 2-amino-isobutyric acid
(Aib)MOD_RES(20)..(20)Xaa is Leu, norleucine (Nle), homoleucine
(Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg, or peptide
bond isosteres thereofMOD_RES(22)..(22)Cys is optionally modified
with a synthetic bone targeting compound, a bisphosphonate,
polyAsp, polyGlu, osteopontin, osteocalcin or sialoprotein
peptides, or charged PEG molecules 47Gly Leu Ser Xaa Gly Xaa Xaa
Xaa Xaa Xaa Xaa Asp Arg Xaa Gly Ser 1 5 10 15 Xaa Ser Gly Xaa Gly
Cys 20 4819PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Xaa is a synthetic or natural polymeric
group or combination thereofMOD_RES(2)..(2)Xaa is absent or may be
one or more amino acids from Gly-Leu-Ser-Lys-Gly and/or
substitutions at one or more of these positionsMOD_RES(7)..(7)Xaa
is Lys, a conservative amino acid substitution, any natural or
unnatural amino acid or peptidomimetic that does have a reactive
primary amine on a side chainMOD_RES(19)..(19)Cys is optionally
modified with a synthetic or natural polymeric group or a
combination thereof 48Xaa Xaa Cys Phe Gly Leu Xaa Leu Asp Arg Ile
Gly Ser Met Ser Gly 1 5 10 15 Leu Gly Cys 4918PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Xaa is absent or may be
a peptide fragment or water soluble polymerMOD_RES(2)..(2)Xaa is
Cys or peptide bond isosteres thereofMOD_RES(3)..(3)Xaa is L-Phe,
D-Phe, 3-amino-2-phenylpropionic acid or N-alkylated derivatives
of
PheMOD_RES(4)..(4)Xaa is Gly, tert-butyl-Gly (tBu-Gly), Val, Ser,
Thr or AsnMOD_RES(5)..(5)Xaa is Leu, Ser, Thr or
N-Me-LeuMOD_RES(6)..(6)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid, Arg,
Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
GluMOD_RES(7)..(7)Xaa is Leu and peptide bond isosteres
thereofMOD_RES(10)..(10)Xaa is Ile, tBu-Gly or peptide bond
isosteres thereofMOD_RES(13)..(13)Xaa is Met, Val, Asn,
beta-Cl-Ala, 2-aminobutyric acid (Abu) or 2-amino-isobutyric acid
(Aib)MOD_RES(16)..(16)Xaa is Leu, norleucine (Nle), homoleucine
(Hleu), Val, tert-butyl-Ala (tBu-Ala), Ser, Thr, Arg or peptide
bond isosteres thereofMOD_RES(18)..(18)Cys is optionally modified
with amino acid sequences useful in bone/cartilage targeting,
polyAsp or polyGlu, osteopontin, osteocalcin, sialoprotein or
sequences derived from NPPC or non-CNP polypeptides 49Xaa Xaa Xaa
Xaa Xaa Xaa Xaa Asp Arg Xaa Gly Ser Xaa Ser Gly Xaa 1 5 10 15 Gly
Cys 5022PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Gly is optionally modified with bone
targeting compound, moieties to reduce renal clearance, charged PEG
molecules or hydrophilic polymersMOD_RES(4)..(4)Xaa is Lys or a
conservative amino acid substitution thereof, any natural or
unnatural amino acid, Arg, Gly, 6-hydroxy-norleucine, citrulline
(Cit), Gln, Ser or GluMOD_RES(6)..(6)Xaa is Cys or peptide
isosteres between positions 6 and 7MOD_RES(7)..(7)Xaa is L-Phe,
D-Phe, 3-amino-2-phenylpropionic acid or N-alkylated derivatives of
PheMOD_RES(8)..(8)Xaa is Gly, tert-butyl-Gly, Thr, Ser, Val or
AsnMOD_RES(9)..(9)Xaa is Leu, Ser, Thr or peptide bond isosteres
thereofMOD_RES(10)..(10)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid, Arg,
Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
GluMOD_RES(11)..(11)Xaa is Leu, Asn or peptide bond isosteres
thereofMOD_RES(14)..(14)Xaa is Ile, tert-butyl-Gly (tBu-Gly), Asn
or peptide bond isosteres thereofMOD_RES(15)..(15)Xaa is Gly, Arg,
Ser or AsnMOD_RES(17)..(17)Xaa is Met, Val, Asn, beta-Cl-Ala,
2-aminobutyric acid (Abu) or 2-amino-isobutyric acid
(Aib)MOD_RES(20)..(20)Xaa is Leu, norleucine (Nle), homoleucine
(Hleu), Val, tert-butyl-Ala (tBu-Ala), Arg, Thr, Ser or peptide
bond isoteres thereofMOD_RES(22)..(22)Cys is optionally modified
with bone targeting compound, moieties to reduce renal clearance,
charged PEG molecules or hydrophilic polymers 50Gly Leu Ser Xaa Gly
Xaa Xaa Xaa Xaa Xaa Xaa Asp Arg Xaa Xaa Ser 1 5 10 15 Xaa Ser Gly
Xaa Gly Cys 20 5122PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Xaa is Arg or Glu 51Xaa Leu Ser Lys Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly
Leu Gly Cys 20 5222PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(5)..(5)Xaa is Arg, Gln or Ser 52Gly Leu Ser Lys
Xaa Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser
Gly Leu Gly Cys 20 5322PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(15)..(15)Xaa is Ser, Asn, Arg, or Cit 53Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Xaa Ser 1 5 10 15
Met Ser Gly Leu Gly Cys 20 5422PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(19)..(19)Xaa is Ser, Arg, or Asn 54Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Xaa Leu Gly Cys 20 5522PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(21)..(21)Xaa is Ser, Thr, or Arg 55Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Xaa Cys 20 5622PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog ABINDING(6)..(6)CH2NH 56Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Gly Cys 20 5722PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalogs BBINDING(7)..(7)Xaa replaced with
N-Me-Phe 57Gly Leu Ser Lys Gly Cys Xaa Gly Leu Lys Leu Asp Arg Ile
Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 5822PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog FBINDING(8)..(8)tBu
58Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1
5 10 15 Met Ser Gly Leu Gly Cys 20 5922PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog HBINDING(7)..(7)Xaa
is replaced with NHCH2-CH(Phe)CO 59Gly Leu Ser Lys Gly Cys Xaa Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 6037PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BL - CNP37 60Gln Glu His Pro Asn Ala
Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly 1 5 10 15 Leu Ser Lys Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25 30 Ser Gly
Leu Gly Cys 35 6135PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CA 61Ala Ala Trp Ala Arg Leu Leu Gln
Glu His Pro Asn Ala Gly Leu Ser 1 5 10 15 Lys Gly Cys Phe Gly Leu
Lys Leu Asp Arg Ile Gly Ser Met Ser Gly 20 25 30 Leu Gly Cys 35
6236PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
CB 62Ala Ala Trp Ala Arg Leu Leu Gln Glu His Pro Asn Ala Arg Gly
Leu 1 5 10 15 Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly
Ser Met Ser 20 25 30 Gly Leu Gly Cys 35 6336PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog CC 63Asp Leu Arg
Val Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Gly Leu 1 5 10 15 Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 20 25
30 Gly Leu Gly Cys 35 6427PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANQQ-CNP22 64Gly Ala Asn Gln Gln Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly Cys 20 25 6527PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANRR-CNP22 65Gly Ala Asn Arg Arg Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly Cys 20 25 6627PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANPR-CNP22 66Gly Ala Asn Pro Arg Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly Cys 20 25 6727PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANSS-CNP22 67Gly Ala Asn Ser Ser Gly Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly Cys 20 25 6832PRTArtificial SequeneSythetic
PolypeptideMISC_FEATUREAnalog CDMOD_RES(1)..(1)N-terminus BNP
tailMOD_RES(32)..(32)N-terminus BNP tail 68Ser Pro Lys Met Val Gln
Gly Ser Gly Cys Phe Gly Leu Lys Leu Asp 1 5 10 15 Arg Ile Gly Ser
Met Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 20 25 30
6927PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREGANQQ-CNP22(K4R) - Analog CH 69Gly Ala Asn
Gln Gln Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp
Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 7027PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREGANSS-CNP22(K4R) - Analog
CG 70Gly Ala Asn Ser Ser Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys
Leu 1 5 10 15 Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25
7138PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
CS 71Gly Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Pro
Lys 1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg
Ile Gly Ser 20 25 30 Met Ser Gly Leu Gly Cys 35 7238PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog CT 72Gly Gln Glu
His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Gln Lys 1 5 10 15 Gly
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 20 25
30 Met Ser Gly Leu Gly Cys 35 7338PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CU 73Gly Gln Glu His Pro Asn Ala Arg
Lys Tyr Lys Gly Ala Asn Gln Gln 1 5 10 15 Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 20 25 30 Met Ser Gly Leu
Gly Cys 35 7438PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CW 74Gly Gln Glu His Pro Asn Ala Arg
Lys Tyr Lys Gly Ala Asn Lys Pro 1 5 10 15 Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 20 25 30 Met Ser Gly Leu
Gly Cys 35 7538PRTArtificial SequenceSynthetic Polypeptide 75Gly
Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys 1 5 10
15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser
20 25 30 Met Ser Gly Leu Gly Cys 35 7637PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog CQ 76Gly His His
Ser His Glu Gln His Pro His Gly Ala Asn Gln Gln Gly 1 5 10 15 Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25
30 Ser Gly Leu Gly Cys 35 7738PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CR 77Gly Ala His His Pro His Glu His
Asp Thr His Gly Ala Asn Gln Gln 1 5 10 15 Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 20 25 30 Met Ser Gly Leu
Gly Cys 35 7837PRTArtificial SequencceSynthetic
PolypeptideMISC_FEATUREAnalog CX 78Gly His His Ser His Glu Gln His
Pro His Gly Ala Asn Pro Arg Gly 1 5 10 15 Leu Ser Lys Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25 30 Ser Gly Leu Gly
Cys 35 7936PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CF 79Gly Gln Pro Arg Glu Pro Gln Val
Tyr Thr Leu Pro Pro Ser Gly Leu 1 5 10 15 Ser Lys Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 20 25 30 Gly Leu Gly Cys 35
8039PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
CY 80Gly Gln His Lys Asp Asp Asn Pro Asn Leu Pro Arg Gly Ala Asn
Pro 1 5 10 15 Arg Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp
Arg Ile Gly 20 25 30 Ser Met Ser Gly Leu Gly Cys 35
8137PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
CE 81Gly Glu Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln
Gly 1 5 10 15 Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser Met 20 25 30 Ser Gly Leu Gly Cys 35 8235PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog CZ 82Phe Gly Ile
Pro Met Asp Arg Ile Gly Arg Asn Pro Arg Gly Leu Ser 1 5 10 15 Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly 20 25
30 Leu Gly Cys 35 8340PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog DA 83Gly Lys Arg Thr Gly Gln Tyr Lys
Leu Gly Ser Lys Thr Gly Pro Gly 1 5 10 15 Pro Lys Gly Leu Ser Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile 20 25 30 Gly Ser Met Ser
Gly Leu Gly Cys 35 40 8437PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CK 84Gly Gln Pro Arg Glu Pro Gln Val
Tyr Thr Gly Ala Asn Gln Gln Gly 1 5 10 15 Leu Ser Arg Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25 30 Ser Gly Leu Gly
Cys 35 8536PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CL 85Gly Val Pro Gln Val Ser Thr Ser
Thr Gly Ala Asn Gln Gln Gly Leu 1 5 10 15 Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 20 25 30 Gly Leu Gly Cys 35
8637PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
CM 86Gly Gln Pro Ser Ser Ser Ser Gln Ser Thr Gly Ala Asn Gln Gln
Gly 1 5 10 15 Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser Met 20 25 30 Ser Gly Leu Gly Cys 35 8737PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog CN 87Gly Gln Thr
His Ser Ser Gly Thr Gln Ser Gly Ala Asn Gln Gln Gly 1 5 10 15 Leu
Ser Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25
30 Ser Gly Leu Gly Cys 35 8837PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CO 88Gly Ser Thr Gly Gln Trp His Ser
Glu Ser Gly Ala Asn Gln Gln Gly 1 5 10 15 Leu Ser Arg Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25 30 Ser Gly Leu Gly
Cys 35 8937PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CP 89Gly Ser Ser Ser Ser Ser Ser Ser
Ser Ser Gly Ala Asn Gln Gln Gly 1 5 10 15 Leu Ser Arg Gly Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met 20 25 30 Ser Gly Leu Gly
Cys 35 9022PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Gly is optionally modified with bone
targeting compound, a bisphosphonates, polyAsp, polyGlu,
osteopontin, osteocalcin, or sialoprotein peptides, negatively
charged PEG molecules or natural polymersMOD_RES(6)..(6)Xaa is Cys
or another natural or unnatural amino acidMOD_RES(7)..(7)Xaa is Phe
or another natural or unnatural amino acidMOD_RES(8)..(8)Xaa is
Gly, a larger natural or unnatural amino acid or
peptidomimeticMOD_RES(22)..(22)Cys is optionally modified with bone
targeting compound, a bisphosphonates, polyAsp, polyGlu,
osteopontin, osteocalcin, or sialoprotein peptides, negatively
charged PEG molecules or natural polymers 90Gly Leu Ser Lys Gly Xaa
Xaa Xaa Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 9122PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog JMOD_RES(6)..(6)Xaa is
CH2-NHBINDING(9)..(9)N-MeBINDING(20)..(20)N-Me 91Gly Leu Ser Lys
Gly Xaa Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser
Gly Leu Gly Cys 20 9222PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog
KBINDING(9)..(9)N-MeBINDING(20)..(20)N-Me 92Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 9322PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog
LBINDING(9)..(9)N-MeBINDING(11)..(11)N-MeBINDING(20)..(20)N-Me
93Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1
5 10 15 Met Ser Gly Leu Gly Cys 20 9422PRTArtificial
SequenceSynthetic
PolypeptideMISC_FEATUREAnalog
MBINDING(9)..(9)N-MeBINDING(11)..(11)N-Me 94Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 9522PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog Z 95Gly Leu Ser Arg Gly Cys Tyr Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 9622PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AA 96Gly Leu Ser Arg Gly Cys Phe Val
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 9722PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AB 97Gly Leu Ser Arg Gly Cys Phe Ser
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 9822PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AC 98Gly Leu Ser Arg Gly Cys Phe Thr
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 9922PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AD 99Gly Leu Ser Arg Gly Cys Phe Gly
Thr Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 10022PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AE 100Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Arg Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 10122PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AFMOD_RES(15)..(15)Xaa is Cit 101Gly
Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Xaa Ser 1 5 10
15 Met Ser Gly Leu Gly Cys 20 10222PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AG 102Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Val Ser Gly Leu Gly Cys
20 10322PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AJ 103Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Val Gly Cys
20 10422PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AKMOD_RES(20)..(20)Xaa is tBu-Ala
104Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser
1 5 10 15 Met Ser Gly Xaa Gly Cys 20 10522PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog AT 105Glu Leu Ser
Glu Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Gly Cys 20 10622PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog AVMOD_RES(1)..(1)N-terminus modified
with pentanoic acid 106Glu Leu Ser Glu Gly Cys Phe Gly Leu Lys Leu
Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
10722PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
AWMOD_RES(1)..(1)Heptanoic acid modifying N-terminus 107Glu Leu Ser
Glu Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Gly Cys 20 10822PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BBMOD_RES(1)..(1)N-terminus modified
with heptanoic acid 108Glu Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu
Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
10922PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
BCMOD_RES(1)..(1)N-terminus modified with pentanoic acid 109Glu Leu
Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15
Met Ser Gly Leu Gly Cys 20 11022PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BFMOD_RES(10)..(10)Xaa is Cit 110Gly
Leu Ser Arg Gly Cys Phe Gly Leu Xaa Leu Asp Arg Ile Gly Ser 1 5 10
15 Met Ser Gly Leu Gly Cys 20 11122PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BG 111Gly Leu Ser Arg Gly Cys Phe Gly
Leu Gln Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 11222PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BH 112Gly Leu Ser Arg Gly Cys Phe Gly
Leu Arg Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 11322PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BJ 113Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Asn Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 11422PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BK 114Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Ser Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 11522PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog NMISC_FEATURE(1)..(22)All D-amino
acids 115Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 11622PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog
BDBINDING(9)..(9)N-Me 116Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
11722PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
BNBINDING(11)..(11)N-Me 117Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
11822PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
BEBINDING(20)..(20)N-Me 118Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
11922PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
VBINDING(7)..(7)3,4-dichloro 119Gly Leu Ser Gly Gly Cys Phe Gly Leu
Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
12022PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
XBINDING(7)..(7)3-methyl 120Gly Leu Ser Gly Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20
12122PRTArtificial SequenceSynthetic PolypeptideMISC_FEATUREAnalog
BP 121Gly Leu Ser Arg Ser Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly
Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 12222PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog BO 122Gly Leu Ser
Arg Arg Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met
Ser Gly Leu Gly Cys 20 12322PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog P 123Gly Leu Ser Gly Gly Cys Phe Gly
Leu Arg Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 12422PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BY 124Gly Leu Ser Arg Gly Cys Phe Gly
Leu Ser Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys
20 12522PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BZ 125Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Gln 1 5 10 15 Met Ser Gly Leu Gly Cys
20 12622PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BX 126Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Asn Ser Gly Leu Gly Cys
20 12722PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BQ 127Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Ser Leu Gly Cys
20 12822PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BR 128Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Arg Leu Gly Cys
20 12922PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BS 129Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Arg Gly Cys
20 13022PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BT 130Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Ser Cys
20 13122PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BU 131Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Thr Cys
20 13222PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog BW 132Gly Leu Ser Arg Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Arg Cys
20 13315PRTArtificial SequenceSynthetic
PolypeptideMISC_FEATUREAnalog CJ 133Arg Ser Ser Cys Phe Gly Gly Arg
Ile Asp Arg Ile Gly Ala Cys 1 5 10 15 13422PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)N-terminal linked to
CNP22 by disuccinimidyl glutarate 134Gly Leu Ser Arg Gly Cys Phe
Gly Leu Arg Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly
Cys 20 13522PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)N-terminal linked to CNP22 by Bis-PEO5
135Gly Leu Ser Arg Gly Cys Phe Gly Leu Arg Leu Asp Arg Ile Gly Ser
1 5 10 15 Met Ser Gly Leu Gly Cys 20 13622PRTArtificial
SequenceSynthetic PolypeptideMISC_FEATUREAnalog EMOD_RES(7)..(7)Xaa
is D-Phe 136Gly Leu Ser Lys Gly Cys Xaa Gly Leu Lys Leu Asp Arg Ile
Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 13722PRTArtificial
SequenceSythetic PolypeptideMISC_FEATUREAnalog GMOD_RES(7)..(7)Xaa
is 3-Cl-Phe 137Gly Leu Ser Lys Gly Cys Xaa Gly Leu Lys Leu Asp Arg
Ile Gly Ser 1 5 10 15 Met Ser Gly Leu Gly Cys 20 13818PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Xaa comprises one or
more amino acids selected from Gly-Leu-Ser-Lys-Gly or substitutions
at one or more of these amino acids and further comprises a
hydrophilic or water soluable polymer 138Xaa Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser Met Ser Gly Leu 1 5 10 15 Gly Cys
13917PRTArtificial SequenceSynthetic PolypeptideMOD_RES(1)..(1)Cys
is modified with a natural and/or synthetic
polymerMOD_RES(17)..(17)Cys is modified with a natural and/or
synthetic polymer 139Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly 1 5 10 15 Cys 14022PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Gly is modified with a
natural and/or synthetic polymerMOD_RES(22)..(22)Cys is modified
with a natural and/or synthetic polymer 140Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly Leu
Gly Cys 20 14117PRTArtificial SequenceSynthetic
PolypeptideMOD_RES(1)..(1)Cys is modified with a natural and/or
synthetic polymeric group or combination thereofMOD_RES(5)..(5)Xaa
is Lys or a conservative amino acid substitution thereof, any
natural or unnatural amino acid, Arg, Gly, 6-hydroxy-norleucine,
citrulline (Cit), Gln, Glu or SerMOD_RES(17)..(17)Cys is optionally
modified with a natural and/or synthetic polymeric group or
combination thereof 141Cys Phe Gly Leu Xaa Leu Asp Arg Ile Gly Ser
Met Ser Gly Leu Gly 1 5 10 15 Cys 14219PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Xaa is optionally
modified with bone- or cartilage-targeting moieties, moieties that
reduce renal clearance, hydrophilic polymers, carbohydrates,
hydrophobic acids and combinations thereofMOD_RES(2)..(2)Xaa is
absent or may comprise 1 to 5 amino acids from Gly-Leu-Ser-Lys-Gly,
optionally substituted with a natural or unnatural amino
acidMOD_RES(7)..(7)Xaa is Lys, any natural or unnatural amino acid
or peptidomimetic that does not have a reactive primary amine on a
side chainMOD_RES(19)..(19)Xaa is optionally modified with bone- or
cartilage-targeting moieties, moieties that reduce renal clearance,
hydrophilic polymers, carbohydrates, hydrophobic acids and
combinations thereof 142Xaa Xaa Cys Phe Gly Leu Xaa Leu Asp Arg Ile
Gly Ser Met Ser Gly 1 5 10 15 Leu Gly Cys 14322PRTArtificial
SequenceSynthetic PolypeptideMOD_RES(1)..(1)Gly is optionally
modified with cartilage or bone targeting compounds, a
bisphosphonate, polyAsp, polyGlu, osteopontin peptides, osteocalcin
peptides, sialoprotein peptides, charged PEG molecules or
hydrophilic polymersMOD_RES(4)..(4)Xaa is Lys or a conservative
amino acid substitution thereof, any natural or unnatural amino
acid, Arg, Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
GluMOD_RES(6)..(6)Xaa is Cys or peptide isosteres between positions
6 and 7MOD_RES(7)..(7)Xaa is L-Phe, D-Phe, N-alkylated Phe, or Phe
analogMOD_RES(8)..(8)Xaa is Gly, tert-butyl-Gly, Thr, Ser, Val or
AsnMOD_RES(9)..(9)Xaa is Leu, Ser, Thr or peptide bond isosteres
thereofMOD_RES(10)..(10)Xaa is Lys or a conservative amino acid
substitution thereof, any natural or unnatural amino acid, Arg,
Gly, 6-hydroxy-norleucine, citrulline (Cit), Gln, Ser or
Glumisc_feature(11)..(11)Xaa can be any naturally occurring amino
acidmisc_feature(14)..(14)Xaa can be any naturally occurring amino
acidMOD_RES(15)..(15)Xaa is Gly, Arg, Ser or
Asnmisc_feature(17)..(17)Xaa can be any naturally occurring amino
acidMOD_RES(20)..(20)Xaa is Leu, norleucine (Nle), homoleucine
(Hleu), Val, tert-butyl-Ala (tBu-Ala), Arg, Thr, Ser or peptide
bond isoteres thereofMOD_RES(22)..(22)Cys is optionally modified
with cartilage or bone targeting compounds, a bisphosphonate,
polyAsp, polyGlu, osteopontin peptides, osteocalcin peptides,
sialoprotein peptides, charged PEG molecules or hydrophilic
polymers 143Gly Leu Ser Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Asp Arg Xaa
Xaa Ser 1 5 10 15 Xaa Ser Gly Xaa Gly Cys 20 14438PRTArtificial
SequenceSynthetic Polypeptide 144Gly His Lys Ser Glu Val Ala His
Arg Phe Lys Gly Ala Asn Lys Lys 1 5 10 15 Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 20 25 30 Met Ser Gly Leu
Gly Cys 35 14539PRTArtificial SequenceSynthetic Polypeptide 145Pro
Gly Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys 1 5 10
15 Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly
20 25 30 Ser Met Ser Gly Leu Gly Cys 35 14652PRTArtificial
SequenceSynthetic Polypeptide 146Leu Arg Val Asp Thr Lys Ser Arg
Ala Ala Trp Ala Arg Leu Leu Gln 1 5 10 15 Glu His Pro Asn Ala Arg
Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu 20 25 30 Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 35 40 45 Gly Leu
Gly Cys 50 14751PRTArtificial SequenceSynthetic Polypeptide 147Arg
Val Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln Glu 1 5 10
15 His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser
20 25 30 Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met
Ser Gly 35 40 45 Leu Gly Cys 50
14850PRTArtificial SequenceSynthetic Polypeptide 148Val Asp Thr Lys
Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln Glu His 1 5 10 15 Pro Asn
Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser Lys 20 25 30
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu 35
40 45 Gly Cys 50 14949PRTArtificial SequenceSynthetic Polypeptide
149Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln Glu His Pro
1 5 10 15 Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser
Lys Gly 20 25 30 Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met
Ser Gly Leu Gly 35 40 45 Cys 15048PRTArtificial SequenceSynthetic
Polypeptide 150Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln Glu
His Pro Asn 1 5 10 15 Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly
Leu Ser Lys Gly Cys 20 25 30 Phe Gly Leu Lys Leu Asp Arg Ile Gly
Ser Met Ser Gly Leu Gly Cys 35 40 45 15147PRTArtificial
SequenceSynthetic Polypeptide 151Lys Ser Arg Ala Ala Trp Ala Arg
Leu Leu Gln Glu His Pro Asn Ala 1 5 10 15 Arg Lys Tyr Lys Gly Ala
Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe 20 25 30 Gly Leu Lys Leu
Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40 45
15246PRTArtificial SequenceSynthetic Polypeptide 152Ser Arg Ala Ala
Trp Ala Arg Leu Leu Gln Glu His Pro Asn Ala Arg 1 5 10 15 Lys Tyr
Lys Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly 20 25 30
Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40 45
15345PRTArtificial SequenceSynthetic Polypeptide 153Arg Ala Ala Trp
Ala Arg Leu Leu Gln Glu His Pro Asn Ala Arg Lys 1 5 10 15 Tyr Lys
Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu 20 25 30
Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40 45
15444PRTArtificial SequenceSynthetic Polypeptide 154Ala Ala Trp Ala
Arg Leu Leu Gln Glu His Pro Asn Ala Arg Lys Tyr 1 5 10 15 Lys Gly
Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys 20 25 30
Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40
15543PRTArtificial SequenceSynthetic Polypeptide 155Ala Trp Ala Arg
Leu Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys 1 5 10 15 Gly Ala
Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu 20 25 30
Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40
15642PRTArtificial SequenceSynthetic Polypeptide 156Trp Ala Arg Leu
Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly 1 5 10 15 Ala Asn
Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp 20 25 30
Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 35 40 15741PRTArtificial
SequenceSynthetic Polypeptide 157Ala Arg Leu Leu Gln Glu His Pro
Asn Ala Arg Lys Tyr Lys Gly Ala 1 5 10 15 Asn Lys Lys Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg 20 25 30 Ile Gly Ser Met
Ser Gly Leu Gly Cys 35 40 15840PRTArtificial SequenceSynthetic
Polypeptide 158Arg Leu Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys
Gly Ala Asn 1 5 10 15 Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu
Lys Leu Asp Arg Ile 20 25 30 Gly Ser Met Ser Gly Leu Gly Cys 35 40
15939PRTArtificial SequenceSynthetic Polypeptide 159Leu Leu Gln Glu
His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys 1 5 10 15 Lys Gly
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 20 25 30
Ser Met Ser Gly Leu Gly Cys 35 16038PRTArtificial SequenceSynthetic
Polypeptide 160Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala
Asn Lys Lys 1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu
Asp Arg Ile Gly Ser 20 25 30 Met Ser Gly Leu Gly Cys 35
16136PRTArtificial SequenceSynthetic Polypeptide 161Glu His Pro Asn
Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu 1 5 10 15 Ser Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 20 25 30
Gly Leu Gly Cys 35 16235PRTArtificial SequenceSynthetic Polypeptide
162His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser
1 5 10 15 Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met
Ser Gly 20 25 30 Leu Gly Cys 35 16334PRTArtificial
SequenceSynthetic Polypeptide 163Pro Asn Ala Arg Lys Tyr Lys Gly
Ala Asn Lys Lys Gly Leu Ser Lys 1 5 10 15 Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser Met Ser Gly Leu 20 25 30 Gly Cys
16433PRTArtificial SequenceSynthetic Polypeptide 164Asn Ala Arg Lys
Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly 1 5 10 15 Cys Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly 20 25 30
Cys 16532PRTArtificial SequenceSynthetic Polypeptide 165Ala Arg Lys
Tyr Lys Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys 1 5 10 15 Phe
Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25
30 16631PRTArtificial SequenceSynthetic Polypeptide 166Arg Lys Tyr
Lys Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe 1 5 10 15 Gly
Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 30
16730PRTArtificial SequenceSynthetic Polypeptide 167Lys Tyr Lys Gly
Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly 1 5 10 15 Leu Lys
Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 30
16829PRTArtificial SequenceSynthetic Polypeptide 168Tyr Lys Gly Ala
Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu 1 5 10 15 Lys Leu
Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25
16928PRTArtificial SequenceSynthetic Polypeptide 169Lys Gly Ala Asn
Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys 1 5 10 15 Leu Asp
Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 17027PRTArtificial
SequenceSynthetic Polypeptide 170Gly Ala Asn Lys Lys Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg Ile Gly Ser Met
Ser Gly Leu Gly Cys 20 25 17126PRTArtificial SequenceSynthetic
Polypeptide 171Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly Leu
Lys Leu Asp 1 5 10 15 Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25
17225PRTArtificial SequenceSynthetic Polypeptide 172Asn Lys Lys Gly
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg 1 5 10 15 Ile Gly
Ser Met Ser Gly Leu Gly Cys 20 25 17324PRTArtificial
SequenceSynthetic Polypeptide 173Lys Lys Gly Leu Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile 1 5 10 15 Gly Ser Met Ser Gly Leu
Gly Cys 20 17423PRTArtificial SequenceSynthetic Polypeptide 174Lys
Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 1 5 10
15 Ser Met Ser Gly Leu Gly Cys 20 17521PRTArtificial
SequenceSynthetic Polypeptide 175Leu Ser Lys Gly Cys Phe Gly Leu
Lys Leu Asp Arg Ile Gly Ser Met 1 5 10 15 Ser Gly Leu Gly Cys 20
17620PRTArtificial SequenceSynthetic Polypeptide 176Ser Lys Gly Cys
Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser 1 5 10 15 Gly Leu
Gly Cys 20 17719PRTArtificial SequenceSynthetic Polypeptide 177Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly 1 5 10
15 Leu Gly Cys 17818PRTArtificial SequenceSynthetic Polypeptide
178Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu
1 5 10 15 Gly Cys 17954PRTArtificial SequenceSynthetic Polypeptide
179Gly Asp Leu Arg Val Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu
1 5 10 15 Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn
Lys Lys 20 25 30 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp
Arg Ile Gly Ser 35 40 45 Met Ser Gly Leu Gly Cys 50
18053PRTArtificial SequenceSynthetic Polypeptide 180Asp Leu Arg Val
Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu Leu 1 5 10 15 Gln Glu
His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly 20 25 30
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Asn 35
40 45 Ser Gly Leu Gly Cys 50 18138PRTArtificial SequenceSynthetic
Polypeptide 181Gly Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala
Asn Lys Lys 1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu
Asp Arg Ile Gly Ser 20 25 30 Asn Ser Gly Leu Gly Cys 35
18237PRTArtificial SequenceSynthetic Polypeptide 182Gln Glu His Pro
Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys Gly 1 5 10 15 Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser Asn 20 25 30
Ser Gly Leu Gly Cys 35 18338PRTArtificial SequenceSythetic
Polypeptide 183Gly His Lys Ser Glu Val Ala His Arg Phe Lys Gly Ala
Asn Lys Lys 1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu
Asp Arg Ile Gly Ser 20 25 30 Asn Ser Gly Leu Gly Cys 35
18427PRTArtificial SequenceSynthetic Polypeptide 184Gly Ala Asn Arg
Arg Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys Leu 1 5 10 15 Asp Arg
Ile Gly Ser Asn Ser Gly Leu Gly Cys 20 25 18554PRTArtificial
SequenceSynthetic Polypeptide 185Pro Asp Leu Arg Val Asp Thr Lys
Ser Arg Ala Ala Trp Ala Arg Leu 1 5 10 15 Leu Gln Glu His Pro Asn
Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys 20 25 30 Gly Leu Ser Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 35 40 45 Met Ser
Gly Leu Gly Cys 50 18638PRTArtificial SequenceSynthetic Polypeptide
186Pro Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys
1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser 20 25 30 Met Ser Gly Leu Gly Cys 35 18734PRTArtificial
SequenceSynthetic Polypeptide 187Pro Asn Ala Arg Lys Tyr Lys Gly
Ala Asn Lys Lys Gly Leu Ser Lys 1 5 10 15 Gly Cys Phe Gly Leu Lys
Leu Asp Arg Ile Gly Ser Met Ser Gly Leu 20 25 30 Gly Cys
18839PRTArtificial SequenceSynthetic Polypeptide 188Pro Gly His Lys
Ser Glu Val Ala His Arg Phe Lys Gly Ala Asn Lys 1 5 10 15 Lys Gly
Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 20 25 30
Ser Met Ser Gly Leu Gly Cys 35 18928PRTArtificial SequenceSynthetic
Polypeptide 189Pro Gly Ala Asn Arg Arg Gly Leu Ser Arg Gly Cys Phe
Gly Leu Lys 1 5 10 15 Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly
Cys 20 25 19054PRTArtificial SequenceSynthetic Polypeptide 190Met
Asp Leu Arg Val Asp Thr Lys Ser Arg Ala Ala Trp Ala Arg Leu 1 5 10
15 Leu Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys
20 25 30 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser 35 40 45 Met Ser Gly Leu Gly Cys 50 19139PRTArtificial
SequenceSynthetic Polypeptide 191Met Gly Gln Glu His Pro Asn Ala
Arg Lys Tyr Lys Gly Ala Asn Lys 1 5 10 15 Lys Gly Leu Ser Lys Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 20 25 30 Ser Met Ser Gly
Leu Gly Cys 35 19238PRTArtificial SequenceSynthetic Polypeptide
192Met Gln Glu His Pro Asn Ala Arg Lys Tyr Lys Gly Ala Asn Lys Lys
1 5 10 15 Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile
Gly Ser 20 25 30 Met Ser Gly Leu Gly Cys 35 19339PRTArtificial
SequenceSynthetic Polypeptide 193Met Gly His Lys Ser Glu Val Ala
His Arg Phe Lys Gly Ala Asn Lys 1 5 10 15 Lys Gly Leu Ser Lys Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 20 25 30 Ser Met Ser Gly
Leu Gly Cys 35 19428PRTArtificial SequenceSynthetic Polypeptide
194Met Gly Ala Asn Arg Arg Gly Leu Ser Arg Gly Cys Phe Gly Leu Lys
1 5 10 15 Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25
19530PRTArtificial SequenceSynthetic Polypeptide 195Met Gly Ser Ser
His His His His His His Ser Ser Gly Leu Val Pro 1 5 10 15 Arg Gly
Ser His Thr Gly Asp Asp Asp Asp Lys His Met Asp 20 25 30
196142PRTArtificial SequenceSynthetic Polypeptide 196Met Val Leu
Thr Lys Lys Lys Leu Gln Asp Leu Val Arg Glu Val Cys 1 5 10 15 Pro
Asn Glu Gln Leu Asp Glu Asp Val Glu Glu Met Leu Leu Gln Ile 20 25
30 Ala Asp Asp Phe Ile Glu Ser Val Val Thr Ala Ala Cys Gln Leu Ala
35 40 45 Arg His Arg Lys Ser Ser Thr Leu Glu Val Lys Asp Val Gln
Leu His 50 55 60 Leu Glu Arg Gln Trp Asn Met Trp Ile Met Gly Ser
Ser His His His 65 70 75 80 His His His Ser Ser Gly Leu Val Pro Arg
Gly Ser His Thr Gly Asp 85 90 95 Asp Asp Asp Lys His Met Asp Pro
Gly Gln Glu His Pro Asn Ala Arg 100 105 110 Lys Tyr Lys Gly Ala Asn
Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly 115 120 125 Leu Lys Leu Asp
Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 130 135 140
19772PRTArtificial SequenceSynthetic Polypeptide 197Val Leu Thr Lys
Lys Lys Leu Gln Asp Leu Val Arg Glu Val Cys Pro 1 5 10 15 Asn Glu
Gln Leu Asp Glu Asp Val Glu Glu Met Leu Leu Gln Ile Ala 20 25 30
Asp Asp Phe Ile Glu Ser Val Val Thr Ala Ala Cys Gln Leu Ala Arg 35
40 45 His Arg Lys Ser Ser Thr Leu Glu Val Lys Asp Val Gln Leu His
Leu 50 55 60 Glu Arg Gln Trp Asn Met Trp Ile 65 70
1989PRTArtificial SequenceSynthetic Polypeptide 198Pro Gly Gln Glu
His Pro Asn Ala Arg 1
5 19930PRTArtificial SequenceSynthetic Polypeptide 199Lys Tyr Lys
Gly Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys Phe Gly 1 5 10 15 Leu
Lys Leu Asp Arg Ile Gly Ser Met Ser Gly Leu Gly Cys 20 25 30
2005PRTArtificial SequenceSynthetic Polypeptide 200Gly Ala Asn Lys
Pro 1 5 2015PRTArtificial SequenceSynthetic Polypeptide 201Gly Ala
Asn Pro Lys 1 5 2025PRTArtificial SequenceSynthetic Polypeptide
202Gly Ala Asn Pro Gln 1 5 2035PRTArtificial SequenceSynthetic
Polypeptide 203Gly Ala Asn Gln Lys 1 5 20441PRTTriakis scyllium
204Arg Leu Leu Lys Asp Leu Ser Asn Asn Pro Leu Arg Phe Arg Gly Arg
1 5 10 15 Ser Lys Lys Gly Pro Ser Arg Gly Cys Phe Gly Val Lys Leu
Asp Arg 20 25 30 Ile Gly Ala Met Ser Gly Leu Gly Cys 35 40
20539PRTTriakis scyllium 205Leu Lys Asp Leu Ser Asn Asn Pro Leu Arg
Phe Arg Gly Arg Ser Lys 1 5 10 15 Lys Gly Pro Ser Arg Gly Cys Phe
Gly Val Lys Leu Asp Arg Ile Gly 20 25 30 Ala Met Ser Gly Leu Gly
Cys 35 20638PRTTriakis scyllium 206Lys Asp Leu Ser Asn Asn Pro Leu
Arg Phe Arg Gly Arg Ser Lys Lys 1 5 10 15 Gly Pro Ser Arg Gly Cys
Phe Gly Val Lys Leu Asp Arg Ile Gly Ala 20 25 30 Met Ser Gly Leu
Gly Cys 35 20722PRTTriakis scyllium 207Gly Pro Ser Arg Gly Cys Phe
Gly Val Lys Leu Asp Arg Ile Gly Ala 1 5 10 15 Met Ser Gly Leu Gly
Cys 20 20841PRTLamna ditropis 208Arg Leu Leu Lys Asp Leu Ser Asn
Asn Pro Leu Arg Phe Lys Gly Arg 1 5 10 15 Ser Lys Lys Gly Pro Ser
Arg Gly Cys Phe Gly Val Lys Leu Asp Arg 20 25 30 Ile Gly Ala Met
Ser Gly Leu Gly Cys 35 40 20939PRTLamna ditropis 209Leu Lys Asp Leu
Ser Asn Asn Pro Leu Arg Phe Lys Gly Arg Ser Lys 1 5 10 15 Lys Gly
Pro Ser Arg Gly Cys Phe Gly Val Lys Leu Asp Arg Ile Gly 20 25 30
Ala Met Ser Gly Leu Gly Cys 35 21038PRTLamna ditropis 210Lys Asp
Leu Ser Asn Asn Pro Leu Arg Phe Lys Gly Arg Ser Lys Lys 1 5 10 15
Gly Pro Ser Arg Gly Cys Phe Gly Val Lys Leu Asp Arg Ile Gly Ala 20
25 30 Met Ser Gly Leu Gly Cys 35 21129PRTLamna ditropis 211Phe Lys
Gly Arg Ser Lys Lys Gly Pro Ser Arg Gly Cys Phe Gly Val 1 5 10 15
Lys Leu Asp Arg Ile Gly Ala Met Ser Gly Leu Gly Cys 20 25
21222PRTLamna ditropis 212Gly Pro Ser Arg Gly Cys Phe Gly Val Lys
Leu Asp Arg Ile Gly Ala 1 5 10 15 Met Ser Gly Leu Gly Cys 20
21341PRTSqualus acanthias 213Arg Leu Leu Gln Asp Leu Ser Asn Asn
Pro Leu Arg Phe Lys Gly Arg 1 5 10 15 Ser Lys Lys Gly Pro Ser Arg
Ser Cys Phe Gly Leu Lys Leu Asp Arg 20 25 30 Ile Gly Ala Met Ser
Gly Leu Gly Cys 35 40 21422PRTSqualus acanthias 214Gly Pro Ser Arg
Ser Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ala 1 5 10 15 Met Ser
Gly Leu Gly Cys 20 21522PRTOryzias latipes 215Gly Trp Asn Arg Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser Gly
Leu Gly Cys 20 21624PRTOryzias latipes 216Pro Met Val Ala Gly Gly
Gly Cys Phe Gly Met Lys Met Asp Arg Ile 1 5 10 15 Gly Ser Ile Ser
Gly Leu Gly Cys 20 21727PRTArothron maculatus 217Gly Arg Ser Ser
Met Val Gly Gly Arg Gly Cys Phe Gly Met Lys Ile 1 5 10 15 Asp Arg
Ile Gly Ser Ile Ser Gly Leu Gly Cys 20 25 21822PRTOryzias latipes
218Gly Gly Met Arg Ser Cys Phe Gly Val Arg Leu Glu Arg Ile Gly Ser
1 5 10 15 Phe Ser Gly Leu Gly Cys 20 21922PRTArothron maculatus
219Gly Gly Leu Arg Ser Cys Phe Gly Val Arg Leu Ala Arg Ile Gly Ser
1 5 10 15 Phe Ser Gly Leu Gly Cys 20 22025PRTOryzias latipes 220Gly
Gly Ser Thr Ser Arg Ser Gly Cys Phe Gly His Lys Met Asp Arg 1 5 10
15 Ile Gly Thr Ile Ser Gly Met Gly Cys 20 25 22124PRTArothron
maculatus 221Gly Gly Ser Ser Arg Ser Gly Cys Phe Gly His Lys Met
Asp Arg Ile 1 5 10 15 Gly Thr Ile Ser Gly Met Gly Cys 20
22239PRTOrnithorhynchus anatinus 222Leu Leu His Asp His Pro Asn Pro
Arg Lys Tyr Lys Pro Ala Asn Lys 1 5 10 15 Lys Gly Leu Ser Lys Gly
Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly 20 25 30 Ser Thr Ser Gly
Leu Gly Cys 35 22322PRTOrnithorhynchus anatinus 223Gly Leu Ser Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Thr Ser
Gly Leu Gly Cys 20 22422PRTArtificial SequenceSynthetic Polypeptide
224Gly Leu Ser Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ala
1 5 10 15 Met Ser Gly Leu Gly Cys 20 22522PRTArtificial
SequenceSynthetic Polypeptide 225Gly Leu Ser Lys Gly Cys Phe Gly
Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Gln Ser Gly Leu Gly Cys
20 22622PRTArtificial SequenceSynthetic Polypeptide 226Gly Leu Ser
Lys Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Ala
Ser Gly Leu Gly Cys 20 22723PRTArtificial SequenceSynthetic
Polypeptide 227Ser Leu Arg Arg Ser Ser Cys Phe Gly Leu Lys Leu Asp
Arg Ile Gly 1 5 10 15 Ser Met Ser Gly Leu Gly Cys 20
22827PRTArtificial SequenceSynthetic Polypeptide 228Gly Leu Ser Lys
Gly Cys Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser 1 5 10 15 Met Ser
Gly Leu Gly Cys Asn Ser Phe Arg Tyr 20 25 22922PRTArtificial
SequenceSynthetic Polypeptide 229Cys Phe Gly Leu Lys Leu Asp Arg
Ile Gly Ser Gln Ser Gly Leu Gly 1 5 10 15 Cys Asn Ser Phe Arg Tyr
20 2305PRTArtificial SequenceSythetnic
Polypeptidemisc_feature(5)..(5)Xaa can be any naturally occurring
amino acid 230Ile Glu Gly Arg Xaa 1 5 2316PRTArtificial
SequenceSynthetic Polypeptidemisc_feature(6)..(6)Xaa can be any
naturally occurring amino acid 231Asp Asp Asp Asp Lys Xaa 1 5
2327PRTArtificial SequenceSynthetic
Polypeptidemisc_feature(2)..(3)Xaa can be any naturally occurring
amino acidmisc_feature(5)..(5)Xaa can be any naturally occurring
amino acid 232Glu Xaa Xaa Tyr Xaa Gln Gly 1 5 2334PRTArtificial
SequenceSynthetic Polypeptide 233Val Asp Asp Arg 1
2344PRTArtificial SequenceSynthetic Polypeptide 234Gly Ser Asp Arg
1 2354PRTArtificial SequenceSynthetic Polypeptide 235Ile Thr Asp
Arg 1 2364PRTArtificial SequenceSynthetic Polypeptide 236Pro Gly
Asp Arg 1 2378PRTArtificial SequenceSynthetic Polypeptide 237Ala
Phe Leu Gly Pro Gly Asp Arg 1 5
* * * * *