U.S. patent number PP32,318 [Application Number 16/501,409] was granted by the patent office on 2020-10-13 for cannabis plant named `mr2017002`.
This patent grant is currently assigned to Aurora Cannabis Enterprises Inc.. The grantee listed for this patent is MedReleaf Corp.. Invention is credited to Alan Graziano, Andrew Hand.
United States Patent |
PP32,318 |
Hand , et al. |
October 13, 2020 |
Cannabis plant named `MR2017002`
Abstract
A new cultivar of Cannabis named `MR2017002` that is
characterized by 13% THC and 0.1% CBD by dry weight, significant
resistance to microbial growth, and a low number of intra-flower
leaves on average for Cannabis.
Inventors: |
Hand; Andrew (Bradford,
CA), Graziano; Alan (Toronto, CA) |
Applicant: |
Name |
City |
State |
Country |
Type |
MedReleaf Corp. |
Toronto, Ontario |
N/A |
CA |
|
|
Assignee: |
Aurora Cannabis Enterprises
Inc. (Edmonton, CA)
|
Family
ID: |
68099276 |
Appl.
No.: |
16/501,409 |
Filed: |
April 9, 2019 |
Prior Publication Data
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Document
Identifier |
Publication Date |
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US 20190313563 P1 |
Oct 10, 2019 |
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Foreign Application Priority Data
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Apr 10, 2018 [CA] |
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PBR 18-9418 |
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Current U.S.
Class: |
PLT/258 |
Current CPC
Class: |
A01H
6/28 (20180501); A61K 36/185 (20130101) |
Current International
Class: |
A01H
5/02 (20180101); A01H 6/28 (20180101); A61K
36/185 (20060101) |
Field of
Search: |
;PLT/258,263.1 |
Primary Examiner: Hwu; June
Attorney, Agent or Firm: Christensen O'Connor Johnson
Kindness PLLC
Claims
We claim:
1. A new and distinct cultivar of Cannabis plant named `MR2017002`
as described and illustrated herein.
Description
Genus and species: Cannabis sativa.
Variety denomination: `MR2017002`.
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of priority under 35 U.S.C. 119
(f) to the application for Canadian Plant Breeders' Rights which
was filed for the instant plant variety on Apr. 10, 2018,
Application Number 18-9418.
BACKGROUND OF THE NEW PLANT
The present invention relates to a new and distinct variety of
Cannabis grown for use as medical marijuana. The new cultivar is
known botanically as Cannabis sativa and will be referred to
hereinafter by the cultivar name `MR2017002`.
`MR2017002` originated from a controlled cross in Markham, Ontario,
Canada, between the female Cannabis sativa variety `California
Orange` and the male Cannabis sativa variety `Skunk #1`.
Seeds from the cross were sown in Markham, Ontario, Canada and
plants were screened for a number of traits including
tetrahydrocannabinol (THC) and cannabidiol (CBD) levels, yield,
flowering time, disease resistance and flower morphology. In July
2015, a single plant was selected having about 13% THC and 0.1% CBD
by dry weight, significant resistance to microbial growth, and a
low number of intra-flower leaves. The selection was named
`MR2017002`.
In July 2015, `MR2017002` was first asexually propagated by apical
stem cuttings approximately 10 cm long and having multiple
auxiliary meristems, in Markham, Ontario, Canada. `MR2017002` was
found to reproduce true to type in successive generations of
asexual propagation via vegetative cuttings in Markham, Ontario,
Canada.
SUMMARY
The following traits have been repeatedly observed and represent
the distinguishing characteristics of `MR2017002`. `MR2017002` has
not been tested under all possible conditions and phenotypic
differences may be observed with variations in environmental,
climatic, and cultural conditions, without however, any variance in
genotype. 1. `MR2017002` exhibits approximately 13% THC by dry
weight. 2. `MR2017002` exhibits approximately 0.1% CBD by dry
weight. 3. `MR2017002` exhibits resistance to microbial growth. 4.
`MR2017002` exhibits lower number of intra-flower leaves compared
to reference plant Girl Scout Cookies and/or low on average for
Cannabis.
BRIEF DESCRIPTION OF THE PHOTOGRAPHS
The accompanying color photographs illustrate the overall
appearance of `MR2017002`. The photographs are of plants aged 8 to
9 weeks post-floral transition and grown in flower rooms under
standard cultivation methods in Markham, Ontario, Canada, in
February, 2018. The colors shown are as true as can be reasonably
obtained by conventional photographic procedures.
FIG. 1 depicts multiple, whole plant clones of `MR2017002`.
FIG. 2 presents a close-up view of a single inflorescence and
foliage of `MR2017002`.
BOTANICAL DESCRIPTION OF THE PLANT
The following is a detailed botanical description of the new
Cannabis variety named `MR2017002`. Data was collected in Markham,
Ontario, Canada in 2015 and 2016 from plants 8 to 9 weeks
post-floral transition.
Plants were flowered under standard indoor environmental conditions
with High Pressure Sodium lamps. Clones were cut from healthy
mothers and allowed to root for 14 to 18 days prior to being
introduced to the flower room. The room was maintained at 18 hour
days/6 hour nights for a week to allow the cuttings to establish
adequate vegetative tissue. The floral transition was initiated by
switching to 12 hour days and 12 hour nights. Flowering lasted 9
weeks before harvest, at which point morphological measurements
were taken, samples were taken for chemical analysis, and yield was
quantified. Hermaphroditic male flowers, if they show up, will do
so around week 2-4. It's not all at once, they emerge over
time.
Standard in-house developed nutrients were used throughout growth,
and the plants were defoliated and pruned as necessary throughout
the cycle.
Color determinations were made in accordance with the 2017 Munsell
Plant Tissue Color Book, except where general color terms of
ordinary dictionary significance are used. Classification:
Family.--Cannabaceae. Genus.--Cannabis. Species.--Cannabis sativa.
Common name.--Marijuana. Parentage: Female parent.--`California
Orange`. Male parent.--`Skunk #1`. Propagation: Type.--Clonal
propagation from vegetative cuttings. Time to produce a rooted
young plant.--18 days. Root description.--Fine and well-branched.
Rooting habit.--Fine and well-branched. Plant: Use.--Medical
marijuana. Plant height.--80 cm to 110 cm. Plant diameter.--45 cm
to 50 cm. Proportion of female plants.--High. Proportion of male
plants.--N/A. Proportion of hermaphrodite plants.--Very low;
hermaphroditic male flowers, if present, emerge over time around
weeks 2-4. Shipping quality.--High. Storage life.--Long. Stems:
Length.--80 cm to 100 cm, same as overall plant height. Width.--8
mm to 10 mm. Depth of main stem grooves.--Shallow. Color.--2.5 GY
5/6. Texture.--Fibrous and Pubescent. Lateral branch length.--25 cm
to 30 cm for shorter branches, 55 cm to 65 cm for longer branches.
Average number of nodes.--29. Average internode length.--9 cm.
Leaves: Type/form.--Palmately compound. Arrangement.--Alternate.
Attachment.--Petiolate. Leaf width.--14 cm to 16 cm for fully
mature leaves. Leaf length.--12 cm to 13 cm for fully mature
leaves. Number of leaflets per leaf.--5 to 7. Leaflet
shape.--Lanceolate. Leaflet length.--3 cm to 6 cm for shorter
leaflets, 12 cm to 14 cm for longer leaflets. Leaflet width.--0.7
cm to 0.8 cm for shorter leaflets, 1.5 cm to 2.5 cm for longer
leaflets. Leaflet margin.--Serrate. Leaflet apex.--Acuminate.
Leaflet base.--Attenuate. Leaflet color, upper surface.--7.5 GY
3/4. Leaflet color, lower surface.--7.5 GY 5/4. Venation pattern,
upper and lower leaflet surfaces.--Pinnate. Texture (both
surfaces).--Pubescent, primarily on the abaxial side and along leaf
vasculature. Fragrance.--Present, citrus and skunk.
Stipules.--Present, 2 per node on either side of a petiole,
attenuate. Petioles: Length.--4 cm to 5.5 cm. Diameter.--0.3 cm.
Texture.--Pubescent. Color.--A mix of 2.5 GY 5/6 and 5 RP 3/4.
Intensity of petiole anthocyanin.--Very strong. Inflorescence:
Blooming habit.--Short day photoperiod sensitive. Blooming
period.--50 days. Attachment.--Very short pedicel that clusters of
calyces emanate from. Fragrance.--Citrus and skunk. Inflorescence
color.--5 RP 3/4. Number of inflorescences per plant.--45 to 70.
Diameter (cm).--3 cm to 4 cm. Length (cm).--3 cm to 4 cm.
Inflorescence anthocyanin.--Strong presence of Anthocyanin A mix of
7.5 GY 6/10 and 5 RP 3/4. Inflorescence THC content.--Average of
13.41% by dry weight. Reproductive organs: Pistils.--1. Quantity
(of stigmas) per flower.--2. Stigma length.--Small, about 3-5 mm.
Stigma color.--5Y 8/2 before senescence, 7.5 YR 6/6 post
senescence. Trichomes.--Capitate-sessile, capitate-stalked,
bulbous, and non-glandular (cystolith hairs); trichomes are present
on almost all aerial organs but are present at highest
concentration on female flowers (calyxes, bracts, etc.) as well as
subtending intra-flower leaves. Trichome color.--Clear, may turn
cloudy or amber during senescence. Bracht size.--Small, under 1 cm.
Bracht color.--5 RP 3/4. Pollen.--Absent. Disease and insect/pest
resistance: `MR2017002` exhibits resistance to aerobic bacteria,
yeast and mold, and coliform bacteria as shown below in Table
1.
TABLE-US-00001 TABLE 1 `MR2017002`Disease resistance Average
Aerobic Bacteria Count per plant, 653 in colony forming units
(CFUs) Average Yeast and Mold Count per plant, in CFUs 600 Average
coliform bacteria count per plant, in CFUs 217
COMPARISON WITH PARENTAL LINES AND KNOWN VARIETY
`MR2017002` may be compared with its parental lines as follows.
Whereas `MR2017002` exhibits 13% THC by dry weight, the female
parent exhibits 15% THC by dry weight and the male parent exhibits
8% THC by dry weight. Total THC and Total CBD were quantified with
a Waters LC-MS/MS, running an Acetonitrile:Methanol:2 propanol
gradient mobile phase containing formic acid through a Raptor
ACR-18, 2.7 um, 2.1.times.150 mm column.
The closest variety of Cannabis known to the inventors is the
variety `Girl Scout Cookies`. `MR2017002` can be distinguished from
`Girl Scout Cookies` by its increased resistance to microbial
growth, as shown in Table 2 below. Variety `MR2017002` can also be
distinguished from the variety `Girl Scout Cookies` on the basis
that it does not contain many intra-flower leaves as `Girl Scout
Cookies`.
TABLE-US-00002 TABLE 2 Comparison with commercial variety `Girl
Scout Cookies` `Girl Scout `MR2017002` Cookies` Average Aerobic
Bacteria Count 653 59270 per plant, in CFUs Average Yeast and Mold
Count 600 52450 per plant, in CFUs Average coliform bacteria count
217 760 per plant, in CFUs
* * * * *