U.S. patent number PP25,575 [Application Number 13/506,624] was granted by the patent office on 2015-05-26 for cornus kousa tree named `pam's mountain bouquet`.
This patent grant is currently assigned to University of Tennessee Research Foundation. The grantee listed for this patent is Richard M. Evans, Robert N. Trigiano, Phillip A. Wadl, Mark T. Windham. Invention is credited to Richard M. Evans, Robert N. Trigiano, Phillip A. Wadl, Mark T. Windham.
United States Patent |
PP25,575 |
Trigiano , et al. |
May 26, 2015 |
**Please see images for:
( Certificate of Correction ) ** |
Cornus kousa tree named `Pam's mountain bouquet`
Abstract
A new and distinct cultivar of flowering dogwood tree, which has
fused bracts is provided. This dogwood tree is botanically known as
Cornus kousa and referred to by the following cultivar name: `Pam's
Mountain Bouquet`.
Inventors: |
Trigiano; Robert N. (Maryville,
TN), Wadl; Phillip A. (Powell, TN), Windham; Mark T.
(Knoxville, TN), Evans; Richard M. (Oak Ridge, TN) |
Applicant: |
Name |
City |
State |
Country |
Type |
Trigiano; Robert N.
Wadl; Phillip A.
Windham; Mark T.
Evans; Richard M. |
Maryville
Powell
Knoxville
Oak Ridge |
TN
TN
TN
TN |
US
US
US
US |
|
|
Assignee: |
University of Tennessee Research
Foundation (Knoxville, TN)
|
Family
ID: |
49513697 |
Appl.
No.: |
13/506,624 |
Filed: |
May 3, 2012 |
Prior Publication Data
|
|
|
|
Document
Identifier |
Publication Date |
|
US 20130298297 P1 |
Nov 7, 2013 |
|
Current U.S.
Class: |
PLT/220 |
Current CPC
Class: |
A01H
5/02 (20130101) |
Current International
Class: |
A01H
5/02 (20060101) |
Field of
Search: |
;PLT/220 |
Other References
Wadl, P.A. et al. "Microsatellites from kousa dogwood (Cornus
kousa)" Molecular Ecology Resources, 2008, pp. 780-782, vol. 8.
cited by applicant .
Wadl, P.A. et al. "Molecular Identification Keys for Cultivars and
Lines of Cornus florida and C. kousa Based on Simple Sequence
Repeat Loci" Journal of the American Society for Horticultural
Science, 2008, pp. 783-793, vol. 133, No. 6. cited by
applicant.
|
Primary Examiner: Grunberg; Anne Marie
Attorney, Agent or Firm: Saliwanchik, Lloyd &
Eisenschenk
Claims
We claim:
1. A new and distinct cultivar of Dogwood tree, Cornus kousa, named
`PAM'S MOUNTAIN BOUQUET`, as illustrated and described.
Description
BACKGROUND OF THE INVENTION
The present invention relates to a new and distinct cultivar of
flowering dogwood tree cultivar, which has fused bracts. This
dogwood tree is botanically known as Cornus kousa and hereinafter
referred to by the following cultivar name: `Pam's Mountain
Bouquet`.
This new dogwood cultivar was discovered in a planting of seedlings
within a cultivated area in Oak Ridge, Tenn. `Pam's Mountain
Bouquet` is a selection from the original seedlings grown in Oak
Ridge, Tenn. from seed gifted by Polly Hill. Asexual reproduction
of `Pam's Mountain Bouquet` by rooting of harvested terminal
cuttings and grafting of axillary buds onto seedling rootstocks in
Oak Ridge, Tenn. and at a nursery located in Belvidere Tenn. have
shown that the unique features of this new dogwood cultivar are
stable and reproduced true-to-type in successive vegetative
generations.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1. Photograph of `Pam's Mountain Bouquet`. Colors in the
photograph may differ from actual colors due to lighting and light
reflectance.
FIGS. 2A and 2B. Close-up Photographs of `Pam's Mountain Bouquet`
bracts.
FIG. 3. Dendrogram illustrating the relatedness of "Pam's Mountain
Bouquet" to other selected Cornus kousa cultivars using 11
microsatellite (SSR; Simple Sequence Repeat) markers. Note:
Cultivar Beni Fuji is disclosed in U.S. Plant. Pat. No. 8,676.
DETAILED DESCRIPTION OF THE NEW VARIETY
A new and distinct cultivar of flowering dogwood tree cultivar,
which has fused bracts is provided. This dogwood tree cultivar is
botanically known as Cornus kousa and referred to by the following
cultivar name: `Pam's Mountain Bouquet`. This cultivar appears to
be resistant to powdery mildew caused by Erisphe pulchra and
dogwood anthracnose caused by Discula destructiva.
This new and distinct dogwood tree cultivar was discovered in a
planting of seedlings within a cultivated area in Oak Ridge, Tenn.
and arose from seed gifted by Ms. Polly Hill. `Pam's Mountain
Bouquet` is a selection from the original seedlings. The instant
cultivar was derived from open-pollinated seeds that were bulked
from maternal parents `Big Apple`, `Snowbird`, `Steeple` and an
unnamed tree and the potential paternal parents `Big Apple`,
`Julian`, `Steeple` and another unnamed tree (Auge et al., 2002).
Thus, it is not possible to ascertain the exact parentage. The
subject dogwood tree cultivar differs from all of the potential
parents in that the instant cultivar has fused bracts, whereas none
of the potential parent cultivars show the same characteristic.
Asexual reproduction of `Pam's Mountain Bouquet` by rooting of
harvested terminal cuttings and grafting of axillary buds onto
seedling rootstocks in Oak Ridge, Tenn. and at a nursery located in
Belvidere, Tenn. has shown that the unique features of this new
dogwood cultivar are stable and reproduced true-to-type in
successive generations.
DETAILED BOTANICAL DESCRIPTION
The following observations, measurements and comparisons describe
this cultivar grown in Oak Ridge, Tenn. Trees used for this
description were about twenty (20) years old. Plant hardiness is
expected to be zones 4-9. The color characteristic descriptions use
color references to The Royal Horticultural Society (R.H.S.) Colour
Chart (published 2001), except where general terms of ordinary
dictionary significance are used. Measurements are provided as an
average (with ranges also provided as indicated).
The following Table 1 shows microsatellite (SSR) markers used to
perform unweighted pair group with arithmetic mean (UPGMA) cluster
analysis of 29 cultivars and lines of Cornus kousa. GenBank
accession numbers are given along with, locus designations, forward
(F) and reverse (R) primer sequences (5'-3' direction), and repeat
motif:
TABLE-US-00001 TABLE 1 GenBank acces- sion no. Locus Primer
sequences (5'-3') Repeat EU544308 CK005 F:GCATTTGTCCTTTGTTTGACAT
(AC).sub.20 (SEQ ID NO: 1) R:TTTTTCGCGAAGTGTTCTCTAC (SEQ ID NO: 2)
EU125522 CK007 F:GAGCCCAGAAGAAGAATATAGAC (AG).sub.8 (SEQ ID NO: 3)
R:ATATAATTGGGTTGGGTTTTG (SEQ ID NO: 4) EU125523 CK015
F:GTCAAATTTTTGATCTTTCTCTCT (CT).sub.10 (SEQ ID NO: 5)
R:GGAGAGACAGAGTACAGTAGAGGT (SEQ ID NO: 6) EU125524 CK029
F:AATTTAGGTTAAGGTTTTGATTTG (TC).sub.8 (SEQ ID NO: 7)
R:AGAGAGAATAGGTTACAGCATCAT (SEQ ID NO: 8) EU125525 CK031
F:TGTCACTGCTTACAGAAACAAT (CT).sub.7 (SEQ ID NO: 9)
R:TATGACGAGATTGTATAAGTTGCT (SEQ ID NO:10) EU125526 CK040
F:CCAAGTCAGTTTGGTAGTAATTC (GT).sub.16 (SEQ ID NO: 11)
R:AGTGCAACTTTTACTTGCTATGT (SEQ ID NO: 12) EU125529 CK048
F:ACCAACCAAAAGAAGTATAAAGAA (TA).sub.6 (SEQ ID NO: 13)
R:CCTATAAATAAGGAGTGATTTGGT (SEQ ID NO: 14) EU544309 CK058
F:CTTAAGTCACAAAGACAATGAAAT (GT).sub.10 (SEQ ID NO: 15)
R:AAGAGAGTTCAGATTTATCTTTGC (SEQ ID NO: 16) EU544310 CK070
F:CTTTTCTACACCCTTAACAAGTG (GT).sub.9 (SEQ ID NO: 17)
R:TAGACAATATGTGCTTAATTGGTT (SEQ ID NO: 18) EU544311 CK071
F:CTGCTCGGTTAAGGTATGTT (TG).sub.9 (SEQ ID NO: 19)
R:TTTAAAGTGCGTTGTATACATAA AT (SEQ ID NO: 20) EU544312 CK072
F:AGCACTCATAGTCCTTGCAC (GT).sub.10 (SEQ ID NO: 21)
R:GTTAAAACGAAGAAGATACAACAA (SEQ ID NO: 22)
TABLE-US-00002 TABLE 2 Characteristics of Pam's Mountain Bouquet'
Color Descriptions are based upon the Royal Horticultural Society's
(RHS) colour chart, 2001. Comparative Variety Generalized `Pam's
Mountain (Milky Character Characteristics Bouquet` Way Select) 1
Tree form upright spreading Spreading- (observation) semi-upright
to semi- spreading upright weeping others 2 Tree height dwarf low
Medium (observation) low (about 3-4 meters; medium spread about 4
-5 high meters, and very high dependent on age and environment) 3
Branch thickness thin medium Medium (measurement) medium (age
dependent) Thickness in the thick middle portion of a plant 4 Color
of current Yellow Green Green Shoot Yellow green 143B 143B
(observation) Green current shoot Grayish green color in the Purple
middle portion Crimson of a plant Brown Others 5 Branch color
Yellow Greyed; Green Greyed; (observation) Yellow green 198B Green
current branch Green 198B color in the Purplish middle portion
crimson of a plant Crimson second year+ Brown Others 6 Dark spots
on Absent Absent Absent Branch Present (observation) presence of
dark spots on the branch 7 Branching Low High Medium (observation)
Medium density of High branching 8 Internode length Short Short
Short (measurement) medium Internode length long in the middle
portion of a plant 9 whole shape of Lanceolate Obovate Obovate
leaves Oblanceolate (observation) Oblong see Fig. 1 whole
Elliptical shape of a leaf in Ovate the middle Obovate portion of a
plant Orbicular Others 10 Shape of leaf Acuminate Acuminate
Acuminate tip(observation) Acute see Fig. 2 Tip obtuse shape of a
leaf in Rotundate the middle Others portion of a plant 11 Shape of
leaf Acuminate Truncate Truncate Base Acute (observation) obtuse
see FIG. 2A Rotundate and FIG. 2B Others Base shape of a leaf in
the middle portion of a plant 12 Shape of leaf Entire Entire Entire
Margin others (observation) shape of a leaf margin in the middle
portion of a plant 13 Leaf rolling Rolling inward Rolling inward
Rolling (observation) Flat inward Rolling outward 14 Leaf curvature
In-curved Flat Flat (observation) Flat Out-curved 15 Leaf margin
None None None Undulation presence (observation) 16 Leaf length
Short Long Medium (measurement) Medium (about 10-14 cm) Length from
the long tip to the base of mature leaf 17 Leaf width Narrow Narrow
Medium (measurement) Medium (about 4-5 cm) The maximum wide width
of mature leaf 18 Leaf thickness Thin Medium Medium (observation)
Medium Thickness of Thick mature leaf 19 Bud color Yellowish
Grayish green Grayish (observation) white 179A Green Color of bud
just Yellow 191A after sprouting Yellow green Green Grayish green
Crimson Others 20 Immature leaf Yellowish Light Green Light Green
color white 135B 135B (observation) Yellow Yellow green Green
Grayish green pink Crimson others 21 Presence of Absent Absent
Absent anthocyanin present (observation) Coloration by anthocyanin
on the immature leaf upperside 22 Color of leaf Yellow Green Green
upperside Yellow green 143B 146A (observation) Green Color of
mature Grayish green leaf upperside Purplish crimson Crimson others
23 Color of leaf Yellow Light Green Medium lowerside Yellow green
146B Green (observation) Light green 137A Color of mature Green
leaf lowerside Dark green Grayish green Purplish crimson others 24
Seasonal change Unchanged Changed Changed of a mature leaf changed
(observation) 25 Color of leaves in Yellow Red Dull Red to autumn
Orange 10C -46A maroon (observation) Crimson 61B others 26 Leaf
variegation Not variegated Not variegated Not (observation)
variegated variegated Variegation on leaf upperside 27 Variegation
Spotted NA NA pattern Splashed (observation) Margined Pattern of
Centered variegation on a Blotched leaf upperside others 28
Variegation color White NA NA (observation) Yellowish white
Greenish white Yellow Yellow Green Green Crimson others 29 seasonal
change Unchanged NA NA of variegation changed color (observation)
30 Hair on leaf None None None upperside Low (observation) Medium
hair density on a high mature leaf upperside 31 Hair on leaf None
None None lowerside Low (observation) Medium hair density on a high
mature leaf lowerside 32 Petiole length Short Short Medium
(measurement) Medium (about 1.5-2.5 Length from Long cm.) the base
of blade Very long to the base petiole 33 Petiole width Narrow
Medium Medium (measurement) Medium (<8 mm) The maximum wide
width of a mature leaf petiole 34 Petiole color Yellowish white
Green Green (observation) Yellow Green 143B 143B Green Crimson
others 35 Inflorescence Corymb Umbel Umbel type Umbel (observation)
Head others 36 Inflorescence Upright Upright Upright direction
Horizontal (observation) pendulous 37 Inflorescence Small Medium
Medium diameter Medium (diagonal mean (observation) large length
including bracts = 7.4 cm.; mean width not including bracts = 5.3
cm) 38 Flower diameter Small Small Small (measurement) Medium Large
Very large 39 Floret diameter Small Small Small (measurement)
Medium Large 40 Floret color White Yellow Greenish (observation)
Yellowish white 150C yellow Greenish yellow 150C Light Green others
41 Bract type Single 83% are FUSED; Single and (observation)
Semi-double 17% are Single unfused Full-double (see Table 3) others
42 Uniformity of Not uniform Not uniform Uniform bract size uniform
(observation) 43 Bract over- Not overlap No overlap -- Slightly
lapping Slightly fused overlap (observation) overlap overlap 44
Bract orientation Ascending Recurved, Horizontal (observation)
Horizontal Reflexed, or Flat arching 45 Bract rolling Rolling
inward Varies (may roll Horizontal
(observation) Horizontal inward or Rolling outward outward 46
Degree of bract Weak strong Weak rolling Medium (observation)
strong 47 Bract curvature In-curved Varies Horizontal (observation)
Horizontal (can be recurved, Out-curved flat, or reflexed) 48 Bract
twisting None None None (observation) Weak Medium strong 49 Whole
shape of Oblong Obovate bracts Elliptical Ovate (observation) Ovate
Obovate Orbicular others 50 Shape of bract Acuminate Acuminate apex
Acute Acuminate (observation) Abtuse Rotundate Emarginated others
51 Bract length Short Medium Medium (measurement) Medium Long 52
Bract width Narrow FUSED Medium (measurement) Medium wide 53 Number
of bracts Few FUSED, but 4 Medium(4) (measurement) Medium(4)
Many(over 10) 54 Bract color (color of bract 155A 155A
(measurement) in full bloom) (immature: 157A) 55 Bract variegation
Not variegated Not variegated Not (observation) variegated
variegated 56 Variegation Margined NA NA pattern Splashed
(observation) Bi-colored Spotted shaded others 57 Variegation
(Color of NA NA color variegation (measurement) pattern of a bract
in full bloom) 58 Pistil color White Yellow green Yellow
(observation) Yellowish Not coded green white Not coded Greenish
white Yellow green Green others 59 Stigma color White Dark Green
Green (observation) Yellowish (Not Coded) (Not white Coded)
Greenish white Yellow green Green others 60 Peduncle Thin Medium
Medium thickness Medium (measurement) thick 61 Peduncle length
Short Long Medium (measurement) Medium (mean of 6.8 cm) long 62
Peduncle color Yellowish white Yellow green Yellow (observation)
yellow 144B green Yellow green 144B Green Crimson brown others 63
Fruit shape Elliptical Globose Globose (observation) Ovate Obovate
Globose others 64 Fruit length Short Medium Medium (measurement)
Medium (about 4 cm) long 65 Fruit width Narrow Medium Medium
(measurement) Medium (about 4 cm) wide 66 Fruit color Yellow
Unripe:143B; Ripe: 33B (observation) Orange Ripe 33B to 43A. to 44A
Crimson Highly variable Highly Purplish black depending on variable
Black ripeness depending others on ripeness 67 Fragrance Absent
Absent Absent (observation) present 68 Seed fertility Sterile High
High (observation) Low Medium high 69 Time to the first Early
Medium Medium flowering Medium (April-mid-May) (observation) late
70 Blooming habit Few Many Many (observation) Medium many 71
Flowering One season One season One season season flowering
flowering flowering (observation) Recurrent blooming others 72
Flowering time Early Medium Medium (observation) Medium late 73
Deciduous or Deciduous Deciduous Deciduous evergreen Half-deciduous
(observation) evergreen 74 Cold hardiness Weak Medium Medium
(observation) Medium (to -20.degree. C.--no strong effect) 75 Heat
tolerance Weak Strong Strong (observation) Medium (to 40.degree.
C.--no strong effect) 76 Pest resistance Weak Strong Strong
(observation) Medium (no specific pests strong noted; resistant to
dogwood anthracnose and powdery mildew) 77 Disease Weak Strong
Strong resistance Medium (observation) strong 78 79 Bark color n/a
Grayed-Green Not 198B observed 80 Bark texture n/a Smooth Not
observed 81 Angle of n/a 20.degree.-30.degree. from Not Emerging
vertical stem observed Branches 82 Time to first leaf n/a Mid- to
late-April Not bud burst observed 83 Leaf Vein color n/a
Green-Greyed Not 192A observed 84 Immature Leaf n/a Similar to
fully Not color expanded leaf observed color 85 Bract base n/a
Truncate Not observed 86 Bract margin n/a Entire Not observed 87
Vestiture n/a Puberulous, Not reticulate observed 88 Flower/ n/a
Mean = 34 Not inflorescensce observed number 89 Seed shape n/a
Flattened along Not length observed 90 Seed color n/a Greyed Yellow
Not 162D observed 91 Seed number n/a 0-17 per fruit Not observed 92
Bloom duration n/a 3-5 weeks Not (dried, dead observed bracts are
retained as a "collar" on peduncle until fruit fall in Autumn) 93
Time of Fruit n/a Begins mid- to Not Ripening late-August observed
through October 94 Trunk diameter n/a 18 cm at 15 years Not (at
approximately of age observed breast height) 95 Anther color n/a
Greyed-purple Not N186 observed 96 Flower petal n/a Yellow-green
Not color 145C observed 97 Style/Stigma n/a Inconspicuous Not
description observed
Botanical classification: Cornus kousa `Pam's Mountain Bouquet`.
Unique features: This tree features heavy flowering and exhibits
fused bracts. About 82% of all bracts on the cultivar exhibit some
degree of fusion (one side, two sides or three to four sides being
fused; see data in Table 3).
TABLE-US-00003 TABLE 3 Cornus kousa `Pam's Mountain Bouquet` bract
characteristics. One side Two sides 3-4 sides Year Not fused fused
fused fused 2008 (n = 50) 7 (14%) 3 (6%) 12 (24%) 28 (56%) 2009 (n
= 50) 10 (20%) 1 (2%) 4 (8%) 35 (70%) 2011 (n = 50) 9 (18%) 5 (10%)
9 (18%) 27 (54%) Mean 9 (18%) 3 (6%) 8 (16%) 30 (60%)
All categories of fused bracts=82%. Disease susceptibility: None
noted. Powdery mildew caused by Erisphe pulchra and dogwood
anthracnose Discula destructiva were not observed. Nearby C.
florida (flowering dogwood) trees were heavily infested with
powdery mildew, but not dogwood anthracnose. Insect damage: None
noted.
REFERENCES
Auge, R. M., M. T. Windham, J. L. Moore, W. T. Witte, E. Kubikova,
W. E. Klingeman, R. M. Evans, J. H. Reiss, P. C. Flanagan, and A.
M. Saxton. 2002. Leaf curl and water relations of kousa dogwoods
showing resistance to summer stress. J. Environ. Hort. 20
(3):143-147. Wadl, P. A., X. Wang, A. N. Trigiano, J. A. Skinner,
M. T. Windham, T. A. Rinehart, S. M. Reed, V. R. Pantalone and R N.
Trigiano. 2008. Molecular identification key for cultivars and
lines of Cornus florida and C. kousa based on microsatellite loci.
J. Amer. Soc. Hort. Sci. 133 (6): 783-793. Wadl, P. W., X. Wang, B.
E. Scheffler, T. A. Rinehart, and R. N. Trigiano. 2008.
Microsatellites from kousa dogwood (Cornus kousa). Molec. Ecol.
Res. 8:780-782. DOI:10.1111/j.1471-8286.2007.0262.x. Wadl, P. W.,
Windham, M. T., Evans, R., Trigiano, R. N. 2014. Three New
Cultivars of Cornus kousa: Empire, Pam's Mountain Bouquet, and Red
Steeple. HortScience 49 (9):1230-1233.
SEQUENCE LISTINGS
1
22122DNAArtificial SequencePrimer sequence 1gcatttgtcc tttgtttgac
at 22222DNAArtificial SequencePrimer sequence 2tttttcgcga
agtgttctct ac 22323DNAArtificial SequencePrimer sequence
3gagcccagaa gaagaatata gac 23421DNAArtificial SequencePrimer
sequence 4atataattgg gttgggtttt g 21524DNAArtificial SequencePrimer
sequence 5gtcaaatttt tgatctttct ctct 24624DNAArtificial
SequencePrimer sequence 6ggagagacag agtacagtag aggt
24724DNAArtificial SequencePrimer sequence 7aatttaggtt aaggttttga
tttg 24824DNAArtificial SequencePrimer sequence 8agagagaata
ggttacagca tcat 24922DNAArtificial SequencePrimer sequence
9tgtcactgct tacagaaaca at 221024DNAArtificial SequencePrimer
sequence 10tatgacgaga ttgtataagt tgct 241123DNAArtificial
SequencePrimer sequence 11ccaagtcagt ttggtagtaa ttc
231223DNAArtificial SequencePrimer sequence 12agtgcaactt ttacttgcta
tgt 231324DNAArtificial SequencePrimer sequence 13accaaccaaa
agaagtataa agaa 241424DNAArtificial SequencePrimer sequence
14cctataaata aggagtgatt tggt 241524DNAArtificial SequencePrimer
sequence 15cttaagtcac aaagacaatg aaat 241624DNAArtificial
SequencePrimer sequence 16aagagagttc agatttatct ttgc
241723DNAArtificial SequencePrimer sequence 17cttttctaca cccttaacaa
gtg 231824DNAArtificial SequencePrimer sequence 18tagacaatat
gtgcttaatt ggtt 241920DNAArtificial SequencePrimer sequence
19ctgctcggtt aaggtatgtt 202025DNAArtificial SequencePrimer sequence
20tttaaagtgc gttgtataca taaat 252120DNAArtificial SequencePrimer
sequence 21agcactcata gtccttgcac 202224DNAArtificial SequencePrimer
sequence 22gttaaaacga agaagataca acaa 24
* * * * *