U.S. patent number PP24,972 [Application Number 13/694,677] was granted by the patent office on 2014-10-21 for corylus plant named `york`.
This patent grant is currently assigned to State Board of Higher Education on behalf of Oregon State University. The grantee listed for this patent is State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon State University. Invention is credited to Rebecca L. McCluskey, Shawn A. Mehlenbacher, David C. Smith.
United States Patent |
PP24,972 |
Mehlenbacher , et
al. |
October 21, 2014 |
Corylus plant named `York`
Abstract
A new and distinct cultivar of Corylus plant named `York`
characterized by globose plant habit and moderate vigor, green
developing and fully expanded leaves during the spring and summer,
resistance to eastern filbert blight caused by the fungus
Anisogramma anomala (Peck) E. Muller, presence of random amplified
polymorphic DNA markers 152-800 and AA12-850, expression of
incompatibility alleles S.sub.2 and S.sub.21 in the styles, and DNA
fingerprints at 10 of 23 microsatellite marker loci that differ
from both parents OSU 479.027 and OSU 504.065, and from one parent
at an additional 11 marker loci.
Inventors: |
Mehlenbacher; Shawn A.
(Corvallis, OR), Smith; David C. (Corvallis, OR),
McCluskey; Rebecca L. (Corvallis, OR) |
Applicant: |
Name |
City |
State |
Country |
Type |
State of Oregon acting by and through the State Board of Higher
Education on behalf of Oregon State University |
Corvallis |
OR |
US |
|
|
Assignee: |
State Board of Higher Education on
behalf of Oregon State University (Corvallis, OR)
|
Family
ID: |
51019007 |
Appl.
No.: |
13/694,677 |
Filed: |
December 24, 2012 |
Prior Publication Data
|
|
|
|
Document
Identifier |
Publication Date |
|
US 20140189913 P1 |
Jul 3, 2014 |
|
Current U.S.
Class: |
PLT/152 |
Current CPC
Class: |
A01H
5/08 (20130101); A01H 6/54 (20180501) |
Current International
Class: |
A01H
5/00 (20060101) |
Field of
Search: |
;PLT/152 |
Primary Examiner: Grunberg; Anne
Attorney, Agent or Firm: Klarquist Sparkman, LLP
Government Interests
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
This invention was made with government support under Specific
Cooperative Agreement No. 58-5358-4542 awarded by the United States
Department of Agriculture. The government has certain rights in the
invention.
Claims
We claim:
1. A new and distinct cultivar of Corylus plant named `York`, as
illustrated and described.
Description
Botanical denomination: Corylus avellana.
Variety designation: `York`.
BACKGROUND
The present Invention relates to a new and distinct cultivar of
Corylus plant (hazelnut, filbert), botanically known as Corylus
avellana, and hereinafter referred to by the name `York`. Corylus
avellana is in the family Betulaceae.
The new Corylus resulted from a controlled cross of female parent
OSU 479.027 (unpatented) and male parent OSU 504.065 (unpatented)
made in 1997 by Shawn A. Mehlenbacher and David C. Smith. Hybrid
seeds from the cross were harvested in August 1997, stratified, and
seedlings grown in the greenhouse during the summer of 1998. From
this cross, total of 183 seedling trees were planted in the field
in Corvallis, Oreg., USA in October, 1998. `York` was discovered
and selected by the Inventors as a single plant within the progeny
of the stated cross-pollination in a controlled environment in
Corvallis, Oreg.
`York` was originally assigned the designation OSU 878.048, which
indicates the row and tree location of the original seedling. OSU
479.027 is from a cross of OSU 231.111 (unpatented).times.OSU
226.122 (unpatented). OSU 504.065 is from a cross of OSU 186.080
(unpatented).times.VR 17-15 (unpatented). The pedigree of `York`
includes `Barcelona` (unpatented) widely grown in Oregon, `Casina`
from Asturias, Spain (unpatented), `Tonda Gentile delle Langhe`
from Piemonte, northern Italy (unpatented), `Montebello` from
Sicily, Italy (unpatented), and `Tombul Ghiaghli`, a Turkish
cultivar obtained from Greece (unpatented).
The new cultivar was asexually reproduced by rooted suckers
annually for eight years (2003-2010) in Corvallis, Oreg. The new
cultivar was also asexually propagated by whip grafting in
Corvallis, Oreg. The unique features of this new Corylus are stable
and reproduced true-to-type in successive generations of asexual
reproduction.
SUMMARY
The following traits have been repeatedly observed and are
determined to be the unique characteristics of `York`. These
characteristics in combination distinguish `York` as a new and
distinct cultivar: 1. Globose plant habit and moderate vigor. 2.
Green developing and fully expanded leaves during the spring and
summer. 3. Resistance to eastern filbert blight caused by the
fungus Anisogramma anomala (Peck) E. Muller. 4. Presence of random
amplified polymorphic DNA markers 152-800 and AA12-850 in DNA of
`York` amplified by the polymerase chain reaction. These two
markers are linked to a dominant allele for resistance to eastern
filbert blight from the cultivar Gasaway (unpatented). 5.
Expression of incompatibility alleles S.sub.2 and S.sub.21 in the
styles. 6. DNA fingerprints at 10 of 23 microsatellite marker loci
differ from both parents OSU 479.027 and OSU 504.065, and from one
parent at an additional 11 marker loci. The microsatellite primers
are shown in Table 1, and allele sizes are shown in Table 2. DNA
fingerprints of standard cultivars `Barcelona`, `Tonda Gentile
delle Langhe` and `Extra Ghiaghli`, and `Gasaway`, the source of
eastern filbert blight resistance, are also shown in attached Table
2.
In comparisons in two replicated trials conducted in Corvallis,
Oreg., plants of the new Corylus differed from plants of the
Corylus avellana cultivar Barcelona (unpatented), and other
cultivars and selections of Corylus avellana known to the Inventors
primarily in nut size, nut shape, kernel percentage (ratio of
kernel weight to nut weight), frequency of blank nuts (nuts lacking
kernels), time of pollen shed, time of nut maturity, length of the
husk or involucre, and plant size.
BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying colored photographs illustrate the overall
appearance of the new cultivar, showing the colors as true as it is
reasonably possible to obtain in colored reproductions of this
type. Foliage colors in the photographs may differ slightly from
the color values cited in the detailed botanical description which
accurately describe the colors of the new Corylus.
FIG. 1 shows a tree of the new cultivar `York` growing in a field
in August, in Corvallis, Oreg.
FIG. 2 shows the tree of the new cultivar `York` growing in a field
in January, in Corvallis, Oreg.
FIG. 3 shows typical nuts, raw kernels, and blanched kernels of
`York` hazelnut compared to those of `Jefferson` hazelnut.
FIG. 4 shows typical nuts, raw kernels, and blanched kernels of
`York` hazelnut compared to those of `Lewis` hazelnut.
FIG. 5 shows the typical nuts, raw kernels, and blanched kernels of
`York` hazelnut compared to those of `Barcelona` hazelnut and other
hazelnut cultivars.
DETAILED BOTANICAL DESCRIPTION
The cultivar York has not been observed under all possible
environmental conditions. The phenotype may vary somewhat with
variations in environment such as temperature and light intensity,
without, however, any variance in genotype. The aforementioned
photographs and following observations and measurements describe
plants grown in Corvallis, Oreg. under commercial practice outdoors
in the field during the fall, winter and spring. Plants used for
the photographs and description were propagated by tie-off layerage
and growing on their own roots, and about seven years old. In the
following description, color references are made to The Royal
Horticultural Society Colour Chart, 1966 Edition, except where
general terms of ordinary dictionary significance are used.
Botanical classification: Corylus avellana cultivar York.
Parentage: Female, or seed, parent.--Corylus avellana selection OSU
479.027 (unpatented). Male, or pollen, parent.--Corylus avellana
selection OSU 504.065 (unpatented). Propagation (type rooted
suckers): Time to initiate roots.--About 30 days at 20 degrees C.
Time to produce a rooted young plant.--About six months at 22
degrees C. Root description.--Fine to thick; freely branching;
creamy white in color. Propagation (type whip grafting): Time to
budbreak on the scions.--About 14 days at 25.degree. C. Time to
produce a grafted plant.--About six months at 25.degree. C. Plant
description: General appearance.--Perennial shrub. Size.--Plant
height is about 5 meters; plant diameter or spread is about 5
meters. Form.--Spreading plant habit. Growth and branching
habit.--freely branching; about 15 lateral branches develop per
plant. Pinching, i.e., removal of the terminal apices, enhances
branching with lateral branches potentially forming at every node.
Vigor.--Moderate vigor growth habit. Roots.--Fine to thick; freely
branching; creamy white in color. Lenticels.--11 circular within 1
square centimeter (counted on dormant scions). Lateral branch
description: Length.--About 38 cm. Diameter.--About 6 mm. Internode
length.--About 3.2 cm. Texture.--Smooth, glabrous.
Strength.--Strong. Color.--Immature -- 152B, mature -- 152B.
Foliage description: Arrangement.--Alternate, simple Length.--About
10.0 cm. Width.--About 9.3 cm. Shape.--Ablong to ovate.
Apex.--Obtuse to acute. Base.--Cordate. Margin.--Serrate.
Texture.--Upper and lower surfaces -- slightly pubescent. Venation
pattern.--Pinnate. Leaf bud shape.--Globular. Time of leaf bud
burst.--Midseason, 11 days after `Barcelona`. Color.--Developing
foliage: upper surface 146B, lower surfaces: 146C. Fully expanded
foliage, upper surface: Spring and summer, 146A; late summer and
fall, 146A. Fully expanded foliage, lower surface: Spring and
summer, 146C; late summer and fall, 146C. Venation, upper surface:
Spring and summer, 146A; late summer and fall, 146A. Venation,
lower surface: Spring and summer, 148D; late summer and fall, 148D.
Leaf bud, 177C. Petiole description: Length.--About 2.7 cm.
Diameter.--About 1.8 mm. Texture.--Upper and lower surfaces:
pubescent. Color.--Upper surface: Spring and summer, 139D; late
summer and fall, 139D. lower surface: Spring and summer, 139D; late
summer and fall, 139D. Flower description: Male
inflorescences.--Catkins, color prior to elongation 194C. Female
inflorescence.--Style color 047B. Stigma coloration.--047B. Time of
female flowering.--Midseason, 14 days after `Barcelona`. Time of
pollen shed.--Midseason, around the same time as `Daviana`
(unpatented). Involcure description: Involucre
constriction.--Absent. Involucre length.--25% longer than nuts.
Strength of serration of indentation.--Moderate.
Pubescence.--Little Thickness of callus at base.--Moderate callus
at base similar to `Barcelona`. Description of jointing of
bracts.--Involucre slit to the base on one side. Nuts fall free of
the involucre at maturity (95% fall free). Nut description:
Length.--About 18.0 mm Width.--About 19.7 mm. Depth.--About 17.0
mm. Nut shape.--Round. Nut shape index
[(width+depth)/2*length].--1.02. Nut compression index
(width/depth).--1.16. Nut shell color.--164A. Nut weight.--About
2.73 grams to 2.85 grams. Predominant number of fruits per
cluster.--Average 2 nuts per cluster. Stripes on shell.--About 24,
color slightly darker than 164A. Fruit apex.--Slight (not
prominent). Size of the fruit pistil scar.--Very small (0.5
mm.times.1 mm). Nut curvature of the basal scar.--Flat (plane).
Frequency of blank nuts.--9.7%. Time of nut maturity.--About 3 days
earlier that `Barcelona`. Husk length.--About 25% longer than the
nuts. Kernel weight.--About 1.23 grams to 1.32 grams. Kernel
percentage (kernel weight/nut weight).--About 46%. Kernel shape --
globular. Kernel cross section shape.--Circular. Kernel base
shape.--Flat. Lateral grooves.--None. Disease/pest resistance:
Plants of the new Corylus are highly resistant to eastern filbert
blight caused by the fungus Anisogramma anomala (Peck) E. Muller.
Plants of the new Corylus are highly resistant to bud mites
(Phytoptus avellanae Nal.), while plants of `Tonda Gentile delle
Langhe` are highly susceptible, and plants of `Barcelona` are
highly resistant. Temperature tolerance: Tolerates temperatures
from -10 to 38.degree. C. in the field in Corvallis, Oreg.
TABLE-US-00001 TABLE 1 Primers and annealing temperatures for the
24 microsatellite marker loci used to fingerprint `York` and other
hazelnut cultivars. Repeat Locus motif Size T.sub.a n He Ho A613
(TC).sub.13(CA).sub.12 149-177 60 14 0.85 0.85 A614
(TC).sub.17(CA).sub.10 125-156 60 14 0.85 0.85 NNN(CA).sub.6 A616
(AC).sub.11 136-162 60 13 0.85 0.85 A640 (CT).sub.15 354-378 67 11
0.80 0.73 (CA).sub.13 B107 (CT).sub.14 112-151 55 14 0.85 0.80 B617
(GA).sub.15 280-298 60 9 0.80 0.78 B619 (TC).sub.21 146-180 60 14
0.88 0.88 B634 (AG).sub.15 218-238 60 9 0.76 0.76 B657 (AG).sub.15
210-228 60 8 0.84 0.98 B671 (AG).sub.6NN 221-249 60 13 0.86 0.88
(GA).sub.17 B709 (GA).sub.21 219-233 60 8 0.74 0.76 B733
(TC).sub.15 161-183 60 8 0.68 0.68 B741 (GT).sub.5(GA).sub.12
176-194 60 10 0.77 0.78 B749 (TC).sub.12 200-210 60 6 0.60 0.64
B751 (GA).sub.15 141-153 60 7 0.80 0.80 B774 (AG).sub.15 195-213 60
8 0.80 0.80 B776 (GA).sub.17 134-148 60 7 0.71 0.60 B795
(TC).sub.8Ns(CT).sub.7 296-332 60 12 0.76 0.74 Ns(CT).sub.10
Ns(TC).sub.5 C115 (TAA).sub.5 167-226 60 14 0.80 0.80 (GAA).sub.12
KG809 (AGG).sub.6 333-345 55 5 0.66 0.64 KG811 (GA).sub.17 240-278
58 12 0.83 0.82 KG827 (CT).sub.13AA 264-282 67 9 0.78 0.84
(CA).sub.7 KG830 (CT).sub.14 279-311 67 9 0.79 0.78 GTATT
(CA).sub.8 Soman- (AAT).sub.5 54 3 0.60 0.98 G Locus PIC r LG
Primers 5'-3' A613 0.85 0.00 11 Ned-CACACGCCTT GTCACTCTTT (SEQ ID
NO: 1) A614 0.84 0.00 6 Hex-TGGCAGAGCT TTGTCAGCTT (SEQ ID NO: 3)
A616 0.83 0.00 8 Fam-CACTCATACC GCAAACTCCA (SEQ ID NO: 5) A640 0.7
0.04 10 F-TGCCTCTGCA GTTAGTCATC AAATGTAGG (SEQ ID NO: 7) B107 0.83
0.02 10 Ned-GTAGGTGCAC TTGATGTGCTT TAC (SEQ ID NO: 9) B617 0.78
0.01 8 Fam-TCCGTGTTGA GTATGGACGA (SEQ ID NO: 11) B619 0.7 0.00 3
Fam-AGTCGGCTCC CCTTTTCTC (SEQ ID NO: 13) B634 0.73 0.00 4
Hex-CCTGCATCCA GGACTCATTA 60 (SEQ ID NO: 15) B657 0.82 -0.08 11
Ned-GAGAGTGCGT CTTCCTCTGG (SEQ ID NO: 17) B671 0.84 -0.01 9
Hex-TTGCCAGTGC ATACTCTGAT G (SEQ ID NO: 19) B709 0.70 -0.01 5
Ned-CCAAGCACGA ATGAACTCAA (SEQ ID NO: 21) B733 0.63 0.00 7.2
Ned-CACCCTCTTCA CCACCTCAT (SEQ ID NO: 23) B741 0.74 0.00 5
Fam-GTTCACAGGC TGTTGGGTTT (SEQ ID NO: 25) B749 0.51 -0.03 1
Hex-GGCTGACAAC ACAGCAGAAA (SEQ ID NO: 27) B751 0.77 0.01 7.2
Fam-AGCTGGTTCTT CGACATTCC (SEQ ID NO: 29) B774 0.77 0.01 5
Ned-GTTTTGCGAG CTCATTGTCA (SEQ ID NO: 31) B776 0.67 0.07 6
Fam-TGTATGTACA CACGGAGAGA GAGA (SEQ ID NO: 33) B795 0.74 0.01 NA
Fam-GACCCACAAA CAATAACCTA TCTC (SEQ ID NO: 35) C115 0.77 0.00 4
Fam-ATTTTCCGCA GATAATACAGG (SEQ ID NO: 37) KG809 0.60 0.01 4
Hex-AGGCATCAGT TCATCCAA (SEQ ID NO: 39) KG811 0.81 0.01 2
Ned-AAGGCGGCAC TCGCTCAC (SEQ ID NO: 41) KG827 0.75 -0.04 9
Fam-AGAACTCCGA CTAATAATCC TAACCCTTGC (SEQ ID NO: 43) KG830 0.76
0.00 9 Ned-TGGAGGAAGT TTTGAATGGT AGTAGAGGA (SEQ ID NO: 45) Soman-G
0.51 -0.27 NA Hex-TGGCGTTGCA ACATATTCTC (SEQ ID NO: 47) Locus
Primers 5'-3' Reference A613 R-CCCCTTTCACAT Gurcan et al. GTTTGCTT
2010 (SEQ ID NO: 2) A614 R-GCAGTGGAGGA Gurcan et al. TTGCTGACT 2010
(SEQ ID NO: 4) A616 R-ATGGCTTTTGCT Gurcan et al. TCGTTTTG 2010 (SEQ
ID NO: 6) A640 Fam-CGCCATATAATT Gurcan et al. GGGATGCTTGTT 2010 G
(SEQ ID NO: 8) B107 R-AACACCATATTG Boccacci et al. AGTCTTTCAAAG
2005; Gokirmak C (SEQ ID NO: 10) et al. 2009 B617 R TGTTTTTGGTGG
Gurcan et al. AGCGATG 2010 (SEQ ID NO: 12) B619 R-GCGATCTGACCT
Gurcan et al. CATTTTTG 2010 (SEQ ID NO: 14) B634 R-GTGCAGAGGTTG
Gurcan et al. CACTCAAA 2010 (SEQ ID NO: 16) B657 R-AGCCTCACCTCC
Gurcan et al. AACGAAC 2010 (SEQ ID NO: 18) B671 R-ACCAGCTCTGGG
Gurcan et al. CTTAACAC 2010 (SEQ ID NO: 20) B709 R-GCGGGTTCTCGT
Gurcan et al. TGTACACT 2010 (SEQ ID NO: 22) B733 R-CATCCCCTGTTG
Gurcan et al. GAGTTTTC 2010 (SEQ ID NO: 24) B741 R-CGTGTTGCTCAT
Gurcan et al. GTGTTGTG 2010 (SEQ ID NO: 26) B749 R-TCGGCTAGGGTT
Gurcan et al. AGGGTTTT 2010 (SEQ ID NO: 28 B751 R-AAACTCAAATA
Gurcan et al. AAACCCCTGCTC 2010 (SEQ ID NO: 30) B774 R-TGTGTGTGGTCT
Gurcan et al. GTAGGCACT 2010 (SEQ ID NO: 32) B776 R-TGAGGGGAAGA
Gurcan et al. GGTTTGATG 2010 (SEQ ID NO: 34) B795 R-TGGGCATCATCC
Gurcan et al. AGGTCTA 2010 (SEQ ID NO: 36) C115 GTTTCCAGATCT Bassil
et al. GCCTCCATATAA 2005b, T (SEQ ID NO: Gokirmak et al. 38) 2009
KG809 F-GGAAGGTGAGA Gurcan and GAAATCAAGT Mehlenbacher (SEQ ID NO:
40) 2010 KG811 F-GAACAACTGAA Gurcan and GACAGCAAAG Mehlenbacher
(SEQ ID NO: 42) 2010 KG827 GAGGGAGCAAQ Gurcan and TCAAAGTTGAGA
Mehlenbacher AGAAA 2010 (SEQ ID NO: 44) KG830 AAAGCAACTCAT Gurcan
and AGCTGAAGTCCA Mehlenbacher ATCA 2010 (SEQ ID NO: 46) Soman-G
R-GCCATCTTTAGA unpublished AAGTTCGATACAG (SEQ ID NO: 48) Primer
fluorescent tags are FAM, HEX, and NED. Ta: annealing temperature
(.degree. C.) N: number of alleles He: expected heterozygosity Ho:
observed heterozygosity PIC: polymorphism information content r:
estimated null allele frequency LG: linkage group
TABLE-US-00002 TABLE 2 Allele sizes in `York`, its parents, and
four other hazelnut cultivars at 24 microsatellite loci. `Tonda
Gentile Tag Locus `York` 479.027 504.065 delle Langhe` NED A613
157/177 177/177 157/159 151/157 HEX A614 125/158 125/132 148/158
125/135 FAM A616 142/150 142/150 150/150 148/150 FAM A640 362/374
354/374 354/362 354/368 NED B107 122/134 134/146 122/134 134/152
FAM B617 286/290 286/290 286/286 286/296 FAM B619 156/164 156/164
156/164 148/164 HEX B634 226/234 226/226 232/234 226/226 NED B657
220/222 222/226 220/224 218/226 HEX B671 241/247 227/247 241/241
237/241 NED B709 227/231 227/231 227/227 227/227 NED B733 171/179
171/179 173/179 171/173 FAM B741 177/186 177/186 177/184 177/184
HEX B749 208/208 206/208 206/208 206/208 FAM B751 151/153 151/153
143/151 149/153 NED B774 203/209 209/211 203/207 203/211 FAM B776
137/150 137/137 137/150 137/137 FAM B795 330/330 296/330 330/330
312/330 FAM C115 197/197 194/197 194/197 173/173 HEX KG809 336/345
336/339 339/345 336/339 NED KG811 254/254 254/254 254/264 254/264
FAM KG827 266/270 270/282 266/266 266/268 NED KG830 295/295 295/295
295/295 291/295 HEX SMNG 196/200 196/200 196/200 196/200 `Extra Tag
Locus `Barcelona` Ghiaghli` `Gasaway` NED A613 151/159 167/169
159/161 HEX A614 125/131 125/150 143/158 FAM A616 142/150 150/158
148/148 FAM A640 354/374 374/374 362/368 NED B107 112/134 116/116
122/128 FAM B617 286/290 294/296 292/296 FAM B619 156/170 164/174
170/174 HEX B634 226/226 226/226 220/232 NED B657 218/222 210/222
224/228 HEX B671 223/227 227/247 235/247 NED B709 225/233 225/227
227/227 NED B733 171/173 171/171 173/173 FAM B741 177/186 177/184
186/188 HEX B749 208/208 208/208 206/208 FAM B751 143/153 143/147
143/143 NED B774 203/207 195/203 203/209 FAM B776 135/137 135/137
146/150 FAM B795 330/330 296/310 314/316 FAM C115 173/194 182/194
215/218 HEX KG809 336/336 336/339 336/345 NED KG811 258/264 240/242
254/258 FAM KG827 280/282 276/282 270/280 NED KG830 291/295 291/295
291/305 HEX SMNG 196/200 196/200 196/196
REFERENCES:
Bassil N. V., Botta R., Mehlenbacher S. A. 2005a. Microsatellite
markers in hazelnut: Isolation, characterization and cross-species
amplification. J. Amer. Soc. Hort. Sci. 130:543-549. Bassil N. V.,
Botta R., Mehlenbacher S. A. 2005b. Additional microsatellite
markers of the European hazelnut. Acta Hort. 686:105-110. Boccacci
P., Akkak A., Bassil N. V., Mehlenbacher S. A., Botta R. 2005.
Characterization and evaluation of microsatellite loci in European
hazelnut (C. avellana) and their transferability to other Corylus
species. Molec. Ecol. Notes 5:934-937. Boccacci P., Akkak, A. and
Botta, R. 2006. DNA typing and genetic relations among European
hazelnut (Corylus avellana L.) cultivars using microsatellite
markers. Genome 49:598-611. Gokirmak T , Mehlenbacher S. A., Bassil
N. V. 2009. Characterization of European hazelnut (Corylus
avellana) cultivars using SSR markers. Genetic Resources and Crop
Evolution 56:147-172. Gurcan, K., S. A. Mehlenbacher and V.
Erdogan. 2010a. Genetic diversity in hazelnut cultivars from Black
Sea countries assessed using SSR markers. Plant Breeding (available
on-line doi:10.1111/j.1439-0523.2009.01753.x). Gurcan, K., S. A.
Mehlenbacher, N. V. Bassil, P. Boccacci, A. Akkak and R. Botta.
2010b. New microsatellite markers for Corylus avellana from
enriched libraries. Tree Genetics and Genomes (available on-line as
DOI 10.1007/s11295-010-0269-y). Gurcan, K. and S. A. Mehlenbacher.
2010. Development of microsatellite marker loci for European
hazelnut (Corylus avellana L.) from ISSR fragments. Molecular
Breeding (available on-line).
SEQUENCE LISTINGS
1
48120DNAArtificial SequenceSynthetic polynucleotide 1cacacgcctt
gtcactcttt 20220DNAArtificial SequenceSynthetic polynucleotide
2cccctttcac atgtttgctt 20320DNAArtificial SequenceSynthetic
polynucleotide 3tggcagagct ttgtcagctt 20420DNAArtificial
SequenceSynthetic polynucleotide 4gcagtggagg attgctgact
20520DNAArtificial SequenceSynthetic polynucleotide 5cactcatacc
gcaaactcca 20620DNAArtificial SequenceSynthetic polynucleotide
6atggcttttg cttcgttttg 20729DNAArtificial SequenceSynthetic
polynucleotide 7tgcctctgca gttagtcatc aaatgtagg 29825DNAArtificial
SequenceSynthetic polynucleotide 8cgccatataa ttgggatgct tgttg
25924DNAArtificial SequenceSynthetic polynucleotide 9gtaggtgcac
ttgatgtgct ttac 241025DNAArtificial SequenceSynthetic
polynucleotide 10aacaccatat tgagtctttc aaagc 251120DNAArtificial
SequenceSynthetic polynucleotide 11tccgtgttga gtatggacga
201219DNAArtificial SequenceSynthetic polynucleotide 12tgtttttggt
ggagcgatg 191319DNAArtificial SequenceSynthetic polynucleotide
13agtcggctcc ccttttctc 191420DNAArtificial SequenceSynthetic
polynucleotide 14gcgatctgac ctcatttttg 201520DNAArtificial
SequenceSynthetic polynucleotide 15cctgcatcca ggactcatta
201620DNAArtificial SequenceSynthetic polynucleotide 16gtgcagaggt
tgcactcaaa 201720DNAArtificial SequenceSynthetic polynucleotide
17gagagtgcgt cttcctctgg 201819DNAArtificial SequenceSynthetic
polynucleotide 18agcctcacct ccaacgaac 191921DNAArtificial
SequenceSynthetic polynucleotide 19ttgccagtgc atactctgat g
212020DNAArtificial SequenceSynthetic polynucleotide 20accagctctg
ggcttaacac 202120DNAArtificial SequenceSynthetic polynucleotide
21ccaagcacga atgaactcaa 202220DNAArtificial SequenceSynthetic
polynucleotide 22gcgggttctc gttgtacact 202320DNAArtificial
SequenceSynthetic polynucleotide 23caccctcttc accacctcat
202420DNAArtificial SequenceSynthetic polynucleotide 24catcccctgt
tggagttttc 202520DNAArtificial SequenceSynthetic polynucleotide
25gttcacaggc tgttgggttt 202620DNAArtificial SequenceSynthetic
polynucleotide 26cgtgttgctc atgtgttgtg 202720DNAArtificial
SequenceSynthetic polynucleotide 27ggctgacaac acagcagaaa
202820DNAArtificial SequenceSynthetic polynucleotide 28tcggctaggg
ttagggtttt 202920DNAArtificial SequenceSynthetic polynucleotide
29agctggttct tcgacattcc 203023DNAArtificial SequenceSynthetic
polynucleotide 30aaactcaaat aaaacccctg ctc 233120DNAArtificial
SequenceSynthetic polynucleotide 31gttttgcgag ctcattgtca
203221DNAArtificial SequenceSynthetic polynucleotide 32tgtgtgtggt
ctgtaggcac t 213324DNAArtificial SequenceSynthetic polynucleotide
33tgtatgtaca cacggagaga gaga 243420DNAArtificial SequenceSynthetic
polynucleotide 34tgaggggaag aggtttgatg 203524DNAArtificial
SequenceSynthetic polynucleotide 35gacccacaaa caataaccta tctc
243619DNAArtificial SequenceSynthetic polynucleotide 36tgggcatcat
ccaggtcta 193721DNAArtificial SequenceSynthetic polynucleotide
37attttccgca gataatacag g 213825DNAArtificial SequenceSynthetic
polynucleotide 38gtttccagat ctgcctccat ataat 253918DNAArtificial
SequenceSynthetic polynucleotide 39aggcatcagt tcatccaa
184021DNAArtificial SequenceSynthetic polynucleotide 40ggaaggtgag
agaaatcaag t 214118DNAArtificial SequenceSynthetic polynucleotide
41aaggcggcac tcgctcac 184221DNAArtificial SequenceSynthetic
polynucleotide 42gaacaactga agacagcaaa g 214330DNAArtificial
SequenceSynthetic polynucleotide 43agaactccga ctaataatcc taacccttgc
304428DNAArtificial SequenceSynthetic polynucleotide 44gagggagcaa
gtcaaagttg agaagaaa 284529DNAArtificial SequenceSynthetic
polynucleotide 45tggaggaagt tttgaatggt agtagagga
294628DNAArtificial SequenceSynthetic polynucleotide 46aaagcaactc
atagctgaag tccaatca 284720DNAArtificial SequenceSynthetic
polynucleotide 47tggcgttgca acatattctc 204825DNAArtificial
SequenceSynthetic polynucleotide 48gccatcttta gaaagttcga tacag
25
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