U.S. patent number 7,226,913 [Application Number 10/379,154] was granted by the patent office on 2007-06-05 for pharmaceutical compositions having a.sub.2a adenosine receptor agonist activity.
This patent grant is currently assigned to University of Virginia Patent Foundation. Invention is credited to Irving L. Kron, Joel M. Linden, Timothy MacDonald, Mark Okusa, Ian J. Sarembock, W. Michael Scheld, Gail W. Sullivan.
United States Patent |
7,226,913 |
Linden , et al. |
June 5, 2007 |
**Please see images for:
( Certificate of Correction ) ** |
Pharmaceutical compositions having A.sub.2A adenosine receptor
agonist activity
Abstract
The present invention provides compositions for oral
administration of compounds having A.sub.2A adenosine receptor
agonist activity. These compositions are useful for treatment of
inflammatory conditions.
Inventors: |
Linden; Joel M.
(Charlottesville, VA), Sullivan; Gail W. (Charlottesville,
VA), Sarembock; Ian J. (Charlottesville, VA), MacDonald;
Timothy (Charlottesville, VA), Okusa; Mark
(Charlottesville, VA), Kron; Irving L. (Charlottesville,
VA), Scheld; W. Michael (Earlysville, VA) |
Assignee: |
University of Virginia Patent
Foundation (Charlottesville, VA)
|
Family
ID: |
27537509 |
Appl.
No.: |
10/379,154 |
Filed: |
March 3, 2003 |
Prior Publication Data
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Document
Identifier |
Publication Date |
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US 20030162742 A1 |
Aug 28, 2003 |
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Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
Issue Date |
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09827083 |
Mar 11, 2003 |
6531457 |
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09333387 |
May 15, 2001 |
6232297 |
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60135573 |
May 24, 1999 |
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60133374 |
May 10, 1999 |
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60124316 |
Mar 12, 1999 |
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60118029 |
Feb 1, 1999 |
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Current U.S.
Class: |
514/46 |
Current CPC
Class: |
C07H
19/06 (20130101) |
Current International
Class: |
A61K
31/70 (20060101) |
Field of
Search: |
;514/46,424 |
References Cited
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|
Primary Examiner: Spivack; Phyllis G.
Attorney, Agent or Firm: Schwegman, Lundberg, Woessner and
Kluth
Government Interests
The present invention was made with the assistance of U.S.
Government funding (NIH Grant ROL HL37942). The U.S. Government has
certain rights in this invention.
Parent Case Text
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. patent application Ser.
No.: 09/827,083, filed Apr. 5, 2001; now U.S. Pat. No. 6,531,457,
issued Mar. 11, 2003, which is a continuation of U.S. application
Ser. No. 09/333,387, filed Jun. 15, 1999, now U.S. Pat. No.
6,232,297, issued May 15, 2001, which claims priority of U.S.
provisional patent application Ser. Nos. 60/118,029, filed Feb. 1,
1999, 60/124,316, filed Mar. 12, 1999, 60/133,374, filed May 10,
1999 and 60/135,573, filed May 24, 1999.
Claims
What is claimed is:
1. A pharmaceutical composition comprising a compound of the
formula (I): ##STR00007## wherein (a) each R is individually
hydrogen, C.sub.1 C.sub.6 alkyl, C.sub.3 C.sub.7 cycloalkyl, phenyl
or phenyl(C.sub.1 C.sub.3)-alkyl; (b) X is --CH.sub.2OH,
--CO.sub.2R.sup.2, --OC(O)R.sup.2, --CH.sub.2OC(O)R.sup.2 or
--C(O)NR.sup.3R.sup.4; (c) each of R.sup.2, R.sup.3 and R.sup.4 is
individually H, C.sub.1-6-alkyl; C.sub.1-6-alkyl substituted with 1
3 C.sub.1-6-alkoxy, C.sub.3 C.sub.7 cycloalkyl,
C.sub.1-6-alkylthio, halogen, hydroxy, amino,
mono(C.sub.1-6-alkyl)amino, di(C.sub.1-6-alkyl)amino, or
C.sub.6-10-aryl, wherein aryl may be substituted with 1 3 halogen,
C.sub.1-6-alkyl, hydroxy, amino, mono(C.sub.1-6-alkyl)amino, or
di(C.sub.1-6-alkyl)amino; C.sub.6-10-aryl; or C.sub.6-10-aryl
substituted with 1 3 halogen, hydroxy, amino,
mono(C.sub.1-6-alkyl)amino, di(C.sub.1-6-alkyl)amino or
C.sub.1-6-alkyl; (d) R.sup.1 is (X-(Z)-).sub.n[(C.sub.3
C.sub.10)cycloalkyl]-(Z')- wherein Z and Z' are individually
(C.sub.1 C.sub.6)alkyl, optionally interrupted by 1 3 S or
non-peroxide O, or is absent, and n is 1 3; or a pharmaceutically
acceptable salt thereof; in combination with a carrier; wherein the
composition is suitable for oral administration.
2. The composition of claim 1 wherein 5'-X is --CH.sub.2OH or
--C(O)NR.sup.3R.sup.4.
3. The composition of claim 2 wherein 5'-X is
--C(O)NR.sup.3R.sup.4.
4. The composition of claim 2 wherein R.sup.3 is H and R.sup.4 is
(C.sub.1 C.sub.4)alkyl.
5. The composition of claim 1 wherein each R is H or (C.sub.1
C.sub.4)alkyl.
6. The composition of claim 1 wherein Z' is --CH.sub.2-- or
--CH.sub.2--CH.sub.2--.
7. The composition of claim 6 wherein Z is --CH.sub.2-- or
--CH.sub.2--CH.sub.2--.
8. The composition of claim 1 wherein C.sub.3 C.sub.10 cycloalkyl
group of R.sup.1 is cyclohexyl or cyclopentyl.
9. The composition of claim 8 wherein X is (C.sub.1
C.sub.4)alkoxycarbonyl, --C(O)NR.sup.3R.sup.4 or acetoxymethyl.
10. The composition of claim 8 wherein X is carboxy.
11. The composition of claim 8 wherein X-Z and Z' are trans.
12. The composition of claim 1 wherein R is H, X is
ethylaminocarbonyl, and R.sup.1 is
2-(4-methoxycarbonylcyclohexylmethyl).
13. The composition of claim 1 wherein R is H, X is
ethylaminocarbonyl, and R.sup.1 is
2-(4-acetoxymethylcyclohexylmethyl).
14. The composition of claim 1 wherein the compound is methyl
4-(3-{9-6-aminopurin-2-yl)}prop-2-ynyl)cyclohexane-carboxylate.
15. The composition of claim 1 further comprising a Type IV
phosphodiesterase inhibitor.
16. The composition of claim 15 wherein the inhibitor is
rolipram.
17. The composition of claim 1 wherein the composition is a powder,
a gelatin capsule, or compressed into a tablet.
18. The composition of claim 1 wherein X is --CH.sub.2OH,
--CO.sub.2R.sup.2, --OC(O)R.sup.2, or --C(O)NR.sup.3R.sup.4.
Description
FIELD OF THE INVENTION
The present invention relates to methods and compositions for
preventing tissue injury, i.e., due to inflammatory activity.
BACKGROUND OF THE INVENTION
The inflammatory response serves the purpose of eliminating harmful
agents from the body. There is a wide range of pathogenic insults
that can initiate an inflammatory response including infection,
allergens, autoimmune stimuli, immune response to transplanted
tissue, noxious chemicals, and toxins, ischemia/reperfusion,
hypoxia, mechanical and thermal trauma. Inflammation normally is a
very localized action which serves in expulsion, attenuation by
dilution, and isolation of the damaging agent and injured tissue.
The body's response becomes an agent of disease when it results in
inappropriate injury to host tissues in the process of eliminating
the targeted agent, or responding to a traumatic insult.
As examples, inflammation is a component of pathogenesis in several
vascular diseases or injuries. Examples include:
ischemia/reperfusion injury (N. G. Frangogiannis et al., in
Myocardial Ischemia: Mechanisms, Reperfusion, Protection, M.
Karmazyn, ed., Birkhuser Verlag (1996) at 236 284; H. S. Sharma et
al., Med. of Inflamm., 6, 175 (1987)), atherosclerosis (R. Ross,
Nature, 362, 801 (1993)), inflammatory aortic aneurysms (N. Girardi
et al., Ann. Thor. Surg., 64, 251 (1997); D. I. Walker et al.,
Brit. J. Surg., 59, 609 (1972); R. L. Pennell et al., J. Vasc.
Surg., 2, 859 (1985)), and restenosis following balloon angioplasty
(see, R. Ross cited above). The cells involved with inflammation
include leukocytes (i.e., the immune system cells--neutrophils,
eosinophils, lymphocytes, monocytes, basophils, macrophages,
dendritic cells, and mast cells), the vascular endothelium,
vascular smooth muscle cells, fibroblasts, and myocytes.
The release of inflammatory cytokines such as tumor necrosis
factor-alpha (TNF.alpha.) by leukocytes is a means by which the
immune system combats pathogenic invasions, including infections.
TNF.alpha. stimulates the expression and activation of adherence
factors on leukocytes and endothelial cells, primes neutrophils for
an enhanced inflammatory response to secondary stimuli and enhances
adherent neutrophil oxidative activity. See, Sharma et al., cited
above. In addition, macrophages/dendritic cells act as accessory
cells processing antigen for presentation to lymphocytes. The
lymphocytes, in turn, become stimulated to act as pro-inflammatory
cytotoxic cells.
Generally, cytokines stimulate neutrophils to enhance oxidative
(e.g., superoxide and secondary products) and nonoxidative (e.g.,
myeloperoxidase and other enzymes) inflammatory activity.
Inappropriate and over-release of cytokines can produce
counterproductive exaggerated pathogenic effects through the
release of tissue-damaging oxidative and nonoxidative products (K.
G. Tracey et al., J. Exp. Med., 167, 1211 (1988); and D. N. Mannel
et al., Rev. Infect. Dis., 9 (suppl. 5), S602 S606 (1987)). For
example, TNF.alpha. can induce neutrophils to adhere to the blood
vessel wall and then to migrate through the vessel to the site of
injury and release their oxidative and non-oxidative inflammatory
products.
Although monocytes collect slowly at inflammatory foci, given
favorable conditions, the monocytes develop into long-term resident
accessory cells and macrophages. Upon stimulation with an
inflammation trigger, monocytes/macrophages also produce and
secrete an array of cytokines (including TNF.alpha.), complement,
lipids, reactive oxygen species, proteases and growth factors that
remodel tissue and regulate surrounding tissue functions.
For example, inflammatory cytokines have been shown to be
pathogenic in: arthritis (C. A. Dinarello, Semin. Immunol., 4, 133
(1992)); ischemia (A. Seekamp et al., Agents-Actions-Supp., 41, 137
(1993)); septic shock (D. N. Mannel et al., Rev. Infect. Dis., 9
(suppl. 5), S602 S606 (1987)); asthma (N. M. Cembrzynska et al.,
Am. Rev. Respir. Dis., 147, 291 (1993)); organ transplant rejection
(D. K. Imagawa et al., Transplantation, 51, 57 (1991); multiple
sclerosis (H. P. Hartung, Ann. Neurol., 33, 591 (1993)); AIDS (T.
Matsuyama et al., AIDS, 5, 1405 (1991)); and in alkali-burned eyes
(F. Miyamoto et al., Opthalmic Res., 30, 168 (1997)). In addition,
superoxide formation in leukocytes has been implicated in promoting
replication of the human immunodeficiency virus (HIV) (S.
Legrand-Poels et al., AIDS Res. Hum. Retroviruses, 6, 1389
(1990)).
It is well known that adenosine and some analogs of adenosine that
nonselectively activate adenosine receptor subtypes decrease
neutrophil production of inflammatory oxidative products (B. N.
Cronstein et al., Ann. N.Y. Acad. Sci., 451, 291 (1985); P. A.
Roberts et al., Biochem. J., 227, 669 (1985); D. J. Schrier et al.,
J. Immunol., 137, 3284 (1986); B. N. Cronstein et al., Clinical
Immunol. and Immunopath., 42, 76 (1987); M. A. Iannone et al., in
Topics and Perspective in Adenosine Research, E. Gerlach et al.,
eds., Springer-Verlag, Berlin, p. 286 (1987); S. T. McGarrity et
al., J. Leukocyte Biol., 44, 411421 (1988); J. De La Harpe et al.,
J. Immunol., 143, 596 (1989); S. T. McGarrity et al., J. Immunol.,
142, 1986 (1989); and C. P. Nielson et al., Br. J. Pharmacol., 97,
882 (1989)). For example, adenosine has been shown to inhibit
superoxide release from neutrophils stimulated by chemoattractants
such as the synthetic mimic of bacterial peptides, f-met-leu-phe
(fMLP), and the complement component C.sub.5a (B. N. Cronstein et
al., J. Immunol., 135, 1366 (1985)). Adenosine can decrease the
greatly enhanced oxidative burst of PMN (neutrophil) first primed
with TNF.alpha. and then stimulated by a second stimulus such as
f-met-leu-phe (G. W. Sullivan et al., Clin. Res., 41, 172A (1993)).
Additionally, it has been reported that adenosine can decrease the
rate of HIV replication in a T-cell line (S. Sipka et al., Acta.
Biochim. Biopys. Hung., 23, 75 (1988)). However, there is no
evidence that in vivo adenosine has anti-inflammatory activity (G.
S. Firestein et al., Clin. Res., 41, 170A (1993); and B. N.
Cronstein et al., Clin. Res., 41, 244A (1993)).
It has been suggested that there is more than one subtype of
adenosine receptor on neutrophils that can have opposite effects on
superoxide release (B. N. Cronstein et al., J. Clin. Invest., 85,
1150 (1990)). The existence of A.sub.2A receptor on neutrophils was
originally demonstrated by Van Calker et al. (D. Van Calker et al.,
Eur. J. Pharmacology, 206, 285 (1991)).
There has been progressive development of compounds that are more
and more potent and/or selective as agonists of A.sub.2A adenosine
receptors (AR) based on radioligand binding assays and
physiological responses. Initially, compounds with little or no
selectivity for A.sub.2A receptors were developed, such as
adenosine itself or 5'-carboxamides of adenosine, such as
5'-N-ethylcarboxamidoadenosine (NECA) (B. N. Cronstein et al., J.
Immunol., 135, 1366 (1985)). Later, it was shown that addition of
2-alkylamino substituents increased potency and selectivity, e.g.,
CV1808 and CGS21680 (M. F. Jarvis et al., J. Pharmacol. Exp. Ther.,
251, 888 (1989)). 2-Alkoxy-substituted adenosine derivatives such
as WRC-0090 are even more potent and selective as agonists at the
coronary artery A.sub.2A receptor (M. Ueeda et al., J. Med. Chem.,
34, 1334 (1991)). The 2-alklylhydrazino adenosine derivatives,
e.g., SHA 211 (also called WRC-0474) have also been evaluated as
agonists at the coronary artery A.sub.2A receptor (K. Niiya et al.,
J. Med. Chem., 35, 4557 (1992)).
There is one report of the combination of relatively nonspecific
adenosine analogs, R-phenylisopropyladenosine (R-PIA) and
2-chloroadenosine (Cl-Ado) with a phosphodiesterase (PDE) inhibitor
resulting in a lowering of neutrophil oxidative activity (M. A.
Iannone et al., Topics and Perspectives in Adenosine Research, E.
Garlach et al., eds., Springer-Verlag, Berlin, pp. 286 298 (1987)).
However, R-PIA and Cl-Ado analogs are actually more potent
activators of A.sub.1 adenosine receptors than of A.sub.2A
adenosine receptors and, thus, are likely to cause side effects due
to activation of A.sub.1 receptors on cardiac muscle and other
tissues causing effects such as "heart block."
R. A. Olsson et al. (U.S. Pat. No. 5,278,150) disclose selective
adenosine A.sub.2 receptor agonists of the formula:
##STR00001## wherein Rib is ribosyl, R.sub.1 can be H and R.sub.2
can be cycloalkyl. The compounds are disclosed to be useful for
treating hypertension, atherosclerosis and as vasodilators.
Olsson et al. (U.S. Pat. No. 5,140,015) disclose certain adenosine
A.sub.2 receptor agonists of formula:
##STR00002## wherein C(X)BR.sub.2 can be CH.sub.2OH and R.sub.1 can
be alkyl- or alkoxyalkyl. The compounds are disclosed to be useful
as vasodilators or an antihypertensives.
Linden et al. (U.S. Pat. No. 5,877,180) is based on the discovery
that certain inflammatory diseases, such as arthritis and asthma,
may be effectively treated by the administration of compounds which
are selective agonists of A.sub.2A adenosine receptors, preferably
in combination with a Type IV phosphodiesterase inhibitor. An
embodiment of the Linden et al. invention provides a method for
treating inflammatory diseases by administering an effective amount
of an A.sub.2A adenosine receptor of the following formula:
##STR00003## wherein R and X are as described in the patent.
In a preferred embodiment, the Linden et al. invention involves the
administration of a Type IV phosphodiesterase (PDE) inhibitor in
combination with the A.sub.2A adenosine receptor agonist. The Type
IV phosphodiesterase (PDE) inhibitor includes racemic and optically
active 4-(polyalkoxyphenyl)-2-pyrrolidones of the following
formula:
##STR00004## wherein R', R.sup.18, R.sup.19 and X are as disclosed
and described in U.S. Pat. No. 4,193,926. Rolipram is an example of
a suitable Type IV PDE inhibitor included within the above
formula.
G. Cristalli (U.S. Pat. No. 5,593,975) discloses 2-arylethynyl,
2-cycloalkylethynyl or 2-hydroxyalkylethynyl derivatives, wherein
the riboside residue is substituted by carboxy amino, or
substituted carboxy amino (R.sub.3HNC(O)--). 2-Alkynylpurine
derivatives have been disclosed in Miyasaka et al. (U.S. Pat. No.
4,956,345), wherein the 2-alkynyl group is substituted with
(C.sub.3 C.sub.16)alkyl. The '975 compounds are disclosed to be
vasodilators and to inhibit platelet aggregation, and thus to be
useful as anti-ischemic, anti-atherosclerosis and anti-hypertensive
agents.
However, a continuing need exists for selective A.sub.2 adenosine
receptor agonists useful for therapeutic applications, that have
reduced side effects.
SUMMARY OF THE INVENTION
The present invention comprises compounds and methods of their use
for the treatment of inflammatory activity in mammalian tissue. The
inflammatory tissue activity can be due to pathological agents or
can be due to physical, chemical or thermal trauma, or the trauma
of medical procedures, such as organ, tissue or cell
transplantation, angioplasty (PCTA), inflammation following
ischemia/reperfusion, or grafting. The present compounds comprise a
novel class of 2-alkynyladenosine derivatives, substituted at the
ethyne position by substituted cycloalkyl moieties. Preferably, the
riboside residue is substituted at the 5'-position ("X") by an
N-alkyl-(or cycloalkyl)carboxyamino ("aminocarbonyl") moiety. Thus,
the present invention provides a method for inhibiting the
inflammatory response in a mammal, such as a human subject, and
protecting the tissue subject to the response, by administering an
effective amount of one or more compounds of the invention.
The compounds of the invention have the following general formula
(I):
##STR00005## wherein (a) each R is individually hydrogen, C.sub.1
C.sub.6 alkyl, C.sub.3 C.sub.7 cycloalkyl, phenyl or
phenyl(C.sub.1-C.sub.3)-alkyl;
(b) X is --CH.sub.2OH, --CO.sub.2OH, --CO.sub.2R.sup.2,
--OC(O)R.sup.2, CH.sub.2OC(O)R.sup.2 or C(O)NR.sup.3R.sup.4;
(c) each of R.sup.2, R.sup.3 and R.sup.4 is individually H,
C.sub.1-6-alkyl; C.sub.1-6-alkyl substituted with 1 3
C.sub.1-6-alkoxy, C.sub.3 C.sub.7 cycloalkyl, C.sub.1-6-alkylthio,
halogen, hydroxy, amino, mono(C.sub.1-6-alkyl)amino,
di(C.sub.1-6-alkyl)amino, or C.sub.6-10-aryl, wherein aryl may be
substituted with 1 3 halogen, C.sub.1-6-alkyl, hydroxy, amino,
mono(C.sub.1-6-alkyl)amino, or di(C.sub.1-6-alkyl)amino;
C.sub.6-10-aryl; or C.sub.6-10-aryl substituted with 1 3 halogen,
hydroxy, amino, mono(C.sub.1-6-alkyl)amino,
di(C.sub.1-6-alkyl)amino, or C.sub.1-6-alkyl;
(d) R.sup.1 is (X-(Z)-).sub.n[(C.sub.3 C.sub.10)cycloalkyl]-(Z')-
wherein Z and Z' are individually (C.sub.1 C.sub.6)alkyl,
optionally interrupted by 1 3 S or non-peroxide O, or is absent,
and n is 1 3; or a pharmaceutically acceptable salt thereof.
The invention provides a compound of formula I for use in medical
therapy, preferably for use in treating or protecting tissue from
inflammation such as an inflammatory response, as well as the use
of a compound of formula I for the manufacture of a medicament for
the treatment of an inflammatory response due to a pathological
condition or symptom in a mammal, such as a human, which is
associated with inflammation.
Although certain A.sub.2A adenosine receptor agonists have been
reported to be vasodilators, and thus to be useful to directly
treat hypertension, thrombus, atherosclerosis and the like, the
tissue-protective activity of the compounds of formula (I) is not
suggested by the prior art.
The invention also includes the use of a combination of these
compounds with type IV phosphodiesterase inhibitors for synergistic
decreases in the inflammatory response of immune cells.
The invention also provides a pharmaceutical composition comprising
an effective amount of the compound of formula I, or a
pharmaceutically acceptable salt thereof, in combination with a
pharmaceutically acceptable diluent or carrier, and optionally, in
combination with a Type IV phosphodiesterase (PDE) inhibitor.
Preferably, the composition is presented as a unit dosage form.
Additionally, the invention provides a therapeutic method for
preventing or treating a pathological condition or symptom in a
mammal, such as a human, wherein the activity of A.sub.2A adenosine
receptors is implicated and agonism of said activity is desired,
comprising administering to a mammal in need of such therapy, an
effective amount of a compound of formula I, or a pharmaceutically
acceptable salt thereof. It is believed that activation of A.sub.2A
adenosine receptors inhibits inflammation by effecting neutrophils,
mast cells, monocytes/macrophages, T-cells and/or eosinophils.
Inhibition of these inflammatory cells results in tissue protection
following tissue insults.
Among the inflammatory responses that can be treated (including
treated prophylactically) with a compound of formula I, optionally
with a Type IV PDE inhibitor, are inflammation due to
(a) autoimmune stimulation (autoimmune diseases), such as lupus
erythematosus, multiple sclerosis, infertility from endometriosis,
type I diabetes mellitus including the destruction of pancreatic
islets leading to diabetes and the inflammatory consequences of
diabetes, including leg ulcers, Crohn's disease, ulcerative
colitis, inflammatory bowel disease, osteoporosis and rheumatoid
arthritis;
(b) allergic diseases such as asthma, hay fever, rhinitis, vernal
conjunctivitis and other eosinophil-mediated conditions;
(c) skin diseases such as psoriasis, contact dermatitis, eczema,
infectious skin ulcers, open wounds, cellulitis;
(d) infectious diseases including sepsis, septic shock,
encephalitis, infectious arthritis, endotoxic shock, gram negative
shock, Jarisch-Herxheimer reaction, shingles, toxic shock, cerebral
malaria, bacterial meningitis, acute respiratory distress syndrome
(ARDS), lyme disease, HIV infection, (TNF.alpha.-enhanced HIV
replication, TNF.alpha. inhibition of reverse transcriptase
inhibitor activity);
(e) wasting diseases: cachexia secondary to cancer and HIV;
(f) organ, tissue or cell transplantation (e.g., bone marrow,
cornea, kidney, lung, liver, heart, skin, pancreatic islets)
including transplant rejection, and graft versus host disease;
(g) adverse effects from drug therapy, including adverse effects
from amphotericin B treatment, adverse effects from
immunosuppressive therapy, e.g., interleukin-2 treatment, adverse
effects from OKT3 treatment, adverse effects from GM-CSF treatment,
adverse effects of cyclosporine treatment, and adverse effects of
aminoglycoside treatment, stomatitis and mucositis due to
immunosuppression;
(h) cardiovascular conditions including circulatory diseases
induced or exasperated by an inflammatory response, such as
ischemia, atherosclerosis, peripheral vascular disease, restenosis
following angioplasty, inflammatory aortic aneurysm, vasculitis,
stroke, spinal cord injury, congestive heart failure, hemorrhagic
shock, ischemia/reperfusion injury, vasospasm following
subarachnoid hemorrhage, vasospasm following cerebrovascular
accident, pleuritis, pericarditis, and the cardiovascular
complications of diabetes;
(i) dialysis, including pericarditis, due to peritoneal
dialysis;
(j) gout; and
(k) chemical or thermal trauma due to bums, acid, alkali and the
like.
Of particular interest and efficacy is the use of the present
compounds to treat inflammatory responses due to organ, tissue or
cell transplantation, i.e., the transplantation of allogeneic or
xenogeneic tissue into a mammalian recipient, autoimmune diseases
and inflammatory conditions due to circulatory pathologies and the
treatment thereof, including angioplasty, stent placement, shunt
placement or grafting. Unexpectedly, it was found that
administration of one or more compounds of formula (I) was
effective after the onset of the inflammatory response, e.g., after
the subject was afflicted with the pathology or trauma that
initiates the inflammatory response.
The invention also includes a method for measuring the response, or
binding a compound of formula I at or to designated A.sub.2A
adenosine receptor sites comprising said receptors, in vivo or in
vitro, with an amount of a compound of formula I effective to bind
to said receptors. Tissue or cells comprising ligand bound receptor
sites can be used to measure the selectively of test compounds for
specific receptor subtypes, the amount of bioactive compound in
blood or other physiological fluids, or can be used as a tool to
identify potential therapeutic agents for the treatment of diseases
or conditions associated with receptor site activation, by
contacting said agents with said ligand-receptor complexes, and
measuring the extent of displacement of the ligand and/or binding
of the agent, or the cellular response to said agent (e.g., cAMP
accumulation).
BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 is a graph depicting the competition by compounds of the
invention for binding to recombinant human A.sub.2A adenosine
receptors.
FIG. 2 is a graph depicting the decrease in human neutrophil
oxidative activity by compounds of the invention.
FIG. 3 is a graph depicting the inhibition of human neutrophil
oxidative activity by a compound of the invention (JMR193) with and
without rolipram.
FIG. 4 is a graph depicting the ability of ZM241385 to counteract
JMR193 inhibited neutrophil oxidative activity.
FIG. 5 is a graph depicting the effect of a compound of the
invention on neutrophil cAMP content and adherence to a biological
surface.
FIG. 6 is a graph depicting the ability of compound DWH-146e to
inhibit PMN superoxide release on a biological surface.
FIG. 7 is a graph depicting the ability of compound DWH-146e to
reduce plasma creatinine following ischemia/reperfusion injury in
rats.
FIG. 8 is a graph depicting the effect of compound ZM241385 on
renal function that had been improved following I/R injury by
DWH-146e.
FIG. 9 is a comparative image of treated and untreated sections of
rat kidney subjected to I/R injury.
FIG. 10 is a graph depicting the effect of DWH-146e on arterial
pO.sub.2 following lung reperfusion injury in rabbits.
FIG. 11 is a graph depicting the effect of DWH-146e on pulmonary
muscular resistance following lung reperfusion injury.
FIG. 12 is a graph depicting the effect of DWH-146e on
mycloperoxidase activity following lung reperfusion injury.
FIG. 13 is a graph depicting the effect of DWH-146e on neointimal
formation after arterial injury in mice.
FIG. 14 is a graph depicting the effect of compounds JMR193,
DWH-146e, and CGS21680 on TNF release from human monocytes.
FIG. 15 is a graph depicting the effect of compound ZM241385 on the
effects of JMR193 on TNF production.
FIG. 16 is a graph depicting the activity of DWH-146e on mean
leukocyte concentration (WBC/mm.sup.2) in a murine peritonitis
model, following injection of zymosan (Zym).
DETAILED DESCRIPTION OF THE INVENTION
The following definitions are used, unless otherwise described.
Halo is fluoro, chloro, bromo, or iodo. Alkyl, alkoxy, aralkyl,
alkylaryl, etc. denote both straight and branched alkyl groups; but
reference to an individual radical such as "propyl" embraces only
the straight chain radical, a branched chain isomer such as
"isopropyl" being specifically referred to. Aryl includes a phenyl
radical or an ortho-fused bicyclic carbocyclic radical having about
nine to ten ring atoms in which at least one ring is aromatic.
Heteroaryl encompasses a radical attached via a ring carbon of a
monocyclic aromatic ring containing five or six ring atoms
consisting of carbon and one to four heteroatoms each selected from
the group consisting of non-peroxide oxygen, sulfur, and N(X)
wherein X is absent or is H, O, (C.sub.1 C.sub.4)alkyl, phenyl or
benzyl, as well as a radical of an ortho-fused bicyclic heterocycle
of about eight to ten ring atoms derived therefrom, particularly a
benz-derivative or one derived by fusing a propylene, trimethylene,
or tetramethylene diradical thereto.
It will be appreciated by those skilled in the art that the
compounds of formula (I) have more than one chiral center and may
be isolated in optically active and racemic forms. Preferably, the
riboside moiety of formula (I) is derived from D-ribose, i.e., the
3',4'-hydroxyl groups are alpha to the sugar ring and the 2' and 5'
groups is beta (3R, 4S, 2R, 5S). When the two groups on the
cyclohexyl group are in the 4-position, they are preferably trans.
Some compounds may exhibit polymorphism. It is to be understood
that the present invention encompasses any racemic,
optically-active, polymorphic, or stereoisomeric form, or mixtures
thereof, of a compound of the invention, which possess the useful
properties described herein, it being well known in the art how to
prepare optically active forms (for example, by resolution of the
racemic form by recrystallization techniques, or enzymatic
techniques, by synthesis from optically-active starting materials,
by chiral synthesis, or by chromatographic separation using a
chiral stationary phase) and how to determine adenosine agonist
activity using the tests described herein, or using other similar
tests which are well known in the art.
Specific and preferred values listed below for radicals,
substituents, and ranges, are for illustration only; they do not
exclude other defined values or other values within defined ranges
for the radicals and substituents.
Specifically, (C.sub.1 C.sub.6)alkyl can be methyl, ethyl, propyl,
isopropyl, butyl, iso-butyl, sec-butyl, pentyl, 3-pentyl, or hexyl.
As used herein, the term "cycloalkyl" encompasses bicycloalkyl
(norbornyl, 2.2.2-bicyclooctyl, etc.) and tricycloalkyl (adamantyl,
etc.), optionally comprising 1 2 N, O or S. Cycloalkyl also
encompasses (cycloalkyl)alkyl. Thus, (C.sub.3 C.sub.6)cycloalkyl
can be cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl;
(C.sub.3 C.sub.6)cycloalkyl(C.sub.1 C.sub.6)alkyl can be
cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl,
cyclohexylmethyl;, 2-cyclopropylethyl, 2-cyclobutylethyl,
2-cyclopentylethyl, or 2-cyclohexylethyl.
(C.sub.1 C.sub.6)alkoxy can be methoxy, ethoxy, propoxy,
isopropoxy, butoxy, iso-butoxy, sec-butoxy, pentoxy, 3-pentoxy, or
hexyloxy; (C.sub.2 C.sub.6)alkenyl can be vinyl, allyl, 1-propenyl,
2-propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1-pentenyl,
2-pentenyl, 3-pentenyl, 4-pentenyl, 1-hexenyl, 2-hexenyl,
3-hexenyl, 4-hexenyl, or 5-hexenyl; (C.sub.2 C.sub.6)alkynyl can be
ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl,
1-pentynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, 1-hexynyl,
2-hexynyl, 3-hexynyl, 4-hexynyl, or 5-hexynyl; (C.sub.1
C.sub.6)alkanoyl can be acetyl, propanoyl or butanoyl; halo(C.sub.1
C.sub.6)alkyl can be iodomethyl, bromomethyl, chloromethyl,
fluoromethyl, trifluoromethyl, 2-chloroethyl, 2-fluoroethyl,
2,2,2-trifluoroethyl, or pentafluoroethyl; hydroxy(C.sub.1
C.sub.6)alkyl can be hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl,
1-hydroxypropyl, 2-hydroxypropyl, 3-hydroxypropyl, 1-hydroxybutyl,
4-hydroxybutyl, 1-hydroxypentyl, 5-hydroxypentyl, 1-hydroxyhexyl,
or 6-hydroxyhexyl; (C.sub.1 C.sub.6)alkoxycarbonyl
(CO.sub.2R.sup.2) can be methoxycarbonyl, ethoxycarbonyl,
propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl,
pentoxycarbonyl, or hexyloxycarbonyl; (C.sub.1 C.sub.6)alkylthio
can be methylthio, ethylthio, propylthio, isopropylthio, butylthio,
isobutylthio, pentylthio, or hexylthio; (C.sub.2
C.sub.6)alkanoyloxy can be acetoxy, propanoyloxy, butanoyloxy,
isobutanoyloxy, pentanoyloxy, or hexanoyloxy; aryl can be phenyl,
indenyl, or naphthyl; and heteroaryl can be furyl, imidazolyl,
triazolyl, triazinyl, oxazoyl, isoxazoyl, thiazolyl, isothiazoyl,
pyraxolyl, pyrrolyl, pyrazinyl, tetrazolyl, puridyl (or its
N-oxide), thientyl, pyrimidinyl (or its N-oxide), indolyl,
isoquinolyl (or its N-oxide) or quinolyl (or its N-oxide).
A specific value for R is amino, monomethylamino or
cyclopropylamino.
A specific value for R.sup.1 is carboxy- or (C.sub.1
C.sub.4)alkoxycarbonyl-cyclohexyl(C.sub.1 C.sub.4)alkyl.
A specific value for R.sup.2 is H or (C.sub.1 C.sub.4)alkyl, i.e.,
methyl or ethyl.
A specific value for R.sup.3 is H, methyl or phenyl.
A specific value for R.sup.4 is H, methyl or phenyl.
A specific value for Z is --CH.sub.2-- or
--CH.sub.2--CH.sub.2--.
A specific value for X is CO.sub.2R.sup.2, (C.sub.2
C.sub.5)alkanoylmethyl or amido.
A specific value for n is 1.
Preferred compounds of formula (I) are those wherein each R is H, X
is ethylaminocarbonyl and R.sub.1 is 4-carboxycyclohexylmethyl
(DWH-146a), R.sup.1 is 4-methoxycarbonylcyclohexylmethyl (DWH-146e)
or R.sup.1 is 4-acetoxymethyl-cyclohexylmethyl (JMR-193). They are
depicted below (DWH-146 (acid) and methylester (e)) and
JMR-193.
##STR00006##
The synthesis of methyl
4[3-(6-amino-9(5-[(ethylamino)carbonyl]-3,4-dihydroxytetrahydro-Z-furanyl-
-9H-2-purinyl)-2-propynyl]-1-cyclohexane-carboxylate (DWH-146e) was
accomplished by the cross coupling of an iodo-adenosine derivative
(N-ethyl-1'-deoxy-1'-(amino-2-iodo-9H-purin-9-yl)-.beta.-D-ribofuranuoram-
ide) with methyl 4-(2-propynyl)-1-cyclohexanecarboxylate by
utilization of a Pd.sup.11 catalyst. The synthesis of the
iodo-adenosine derivative was accomplished from guanosine.
Guanosine is first treated with acetic anhydride, which acetalates
the sugar hydroxyls, followed by the chlorination of position 6
with tetramethyl ammonium chloride and phosphorousoxychloride.
Iodination of position 2 was accomplished via a modified Sandmeyer
reaction, followed by displacement of the 6-Cl and sugar acetates
with ammonia. The 2' and 3' hydroxyls were protected as the
acetonide and the 5' hydroxyl was iodized to the acid with
potassium permanganate. Deprotection of the 2' and 3' acetonide,
Fisher esterification of the 5' acid with ethanol and conversion of
the resulting ethyl ester to the ethyl amide with ethylamine gave
N-ethyl-1'-deoxy-1'-(amino-2-iodo-9H-purin-9-yl)-.beta.-D-ribofuranuorami-
de.
The acetylene (methyl 4-(2-propynyl)-1-cyclohexanecarboxylate) was
synthesized starting from trans-1,4-cyclohexanedimethanol.
Initially the trans-diol was monotosylated followed by displacement
of the tosylate with an acetylene anion. The hydroxyl of the
resulting hydroxyl acetylene species was oxidized to the acid via
Jones reagent followed by methylation with
(trimethylsilyl)diazomethane to give methyl
4-(2-propynyl)-1-cyclohexanecarboxylate.
The cross-coupling reaction was performed under the following
previously reported conditions. To a solution of
N,N-dimethylformamide (0.5 mL), acetonitrile (1 mL), triethylamine
(0.25 mL), and
N-ethyl-1'-deoxy-1'-(amino-2-iodo-9H-purin-9-yl)-.beta.-D-ribofuranuroami-
de (25 mg, 0.06 mmol) was added bis(triphenylphosphine)palladium
dichloride (1 mg, 2 mol %) and copper(I)iodide (0.06 mg, 0.5 mol
%). To the resulting mixture was added methyl
4-(2-propynyl)-1-cyclohexanecarboxylate (54 mg, 0.3 mmol) and the
reaction was stirred under N.sub.2 atmosphere for 16 hours. The
solvent was removed under vacuum and the resulting residue was
flash chromatographed in 20% methanol in chloroform (R.sub.f=0.45)
to give 19 mg (off-white solid, m.p. 125.degree. C. (decomposed))
of methyl
4[3-(6-amino-9(5-[(ethylamino)carbonyl]-3,4-dihydroxytetrahydro-Z-furanyl-
)-9H-2-purinyl)-2-propynyl]-1-cyclohexanecarboxylate
(DWH-146e).
DWH-146e and JMR193 are substantially more potent as inhibitors in
inflammatory model systems than the reference compound, CGS21680
(2-[p-(carboxyethyl)-phenyl-ethylamino]5'-N-ethylcarboxamidoadenosine).
For example, DWH-146e is about 80 times more potent at A.sub.2A
receptors and 40 times more selective for A.sub.2A over A.sub.3
receptors than is CGS21680.
Examples of pharmaceutically acceptable salts are organic acid
addition salts formed with acids which form a physiological
acceptable anion, for example, tosylate, methanesulfonate, malate,
acetate, citrate, malonate, tartarate, succinate, benzoate,
ascorbate, .alpha.-ketoglutarate, and .alpha.-glycerophosphate.
Suitable inorganic salts may also be formed, including
hydrochloride, sulfate, nitrate, bicarbonate, and carbonate
salts.
Pharmaceutically acceptable salts may be obtained using standard
procedures well known in the art, for example by reacting a
sufficiently basic compound such as an amine with a suitable acid
affording a physiologically acceptable anion. Alkali metal (for
example, sodium, potassium or lithium) or alkaline earth metal (for
example calcium) salts of carboxylic acids can also be made.
The compounds of formula I can be formulated as pharmaceutical
compositions and administered to a mammalian host, such as a human
patient in a variety of forms adapted to the chosen route of
administration, i.e., orally or parenterally, by intravenous,
intramuscular, topical or subcutaneous routes.
Thus, the present compounds may be systemically administered, e.g.,
orally, in combination with a pharmaceutically acceptable vehicle
such as an inert diluent or an assimilable edible carrier. They may
be enclosed in hard or soft shell gelatin capsules, may be
compressed into tablets, or may be incorporated directly with the
food of the patient's diet. For oral therapeutic administration,
the active compound may be combined with one or more excipients and
used in the form of ingestible tablets, buccal tablets, troches,
capsules, elixirs, suspensions, syrups, wafers, and the like. Such
compositions and preparations should contain at least 0.1% of
active compound. The percentage of the compositions and
preparations may, of course, be varied and may conveniently be
between about 2 to about 60% of the weight of a given unit dosage
form. The amount of active compound in such therapeutically useful
compositions is such that an effective dosage level will be
obtained.
The tablets, troches, pills, capsules, and the like may also
contain the following: binders such as gum tragacanth, acacia, corn
starch or gelatin; excipients such as dicalcium phosphate; a
disintegrating agent such as corn starch, potato starch, alginic
acid and the like; a lubricant such as magnesium stearate; and a
sweetening agent such as sucrose, fructose, lactose or aspartame or
a flavoring agent such as peppermint, oil of wintergreen, or cherry
flavoring may be added. When the unit dosage form is a capsule, it
may contain, in addition to materials of the above type, a liquid
carrier, such as a vegetable oil or a polyethylene glycol. Various
other materials may be present as coatings or to otherwise modify
the physical form of the solid unit dosage form. For instance,
tablets, pills, or capsules may be coated with gelatin, wax,
shellac or sugar and the like. A syrup or elixir may contain the
active compound, sucrose or fructose as a sweetening agent, methyl
and propylparabens as preservatives, a dye and flavoring such as
cherry or orange flavor. Of course, any material used in preparing
any unit dosage form should be pharmaceutically acceptable and
substantially non-toxic in the amounts employed. In addition, the
active compound may be incorporated into sustained-release
preparations and devices.
The active compound may also be administered intravenously or
intraperitoneally by infusion or injection. Solutions of the active
compound or its salts can be prepared in water, optionally mixed
with a nontoxic surfactant. Dispersions can also be prepared in
glycerol, liquid polyethylene glycols, triacetin, and mixtures
thereof and in oils. Under ordinary conditions of storage and use,
these preparations contain a preservative to prevent the growth of
microorganisms.
The pharmaceutical dosage forms suitable for injection or infusion
can include sterile aqueous solutions or dispersions or sterile
powders comprising the active ingredient which are adapted for the
extemporaneous preparation of sterile injectable or infusible
solutions or dispersions, optionally encapsulated in liposomes. In
all cases, the ultimate dosage form must be sterile, fluid and
stable under the conditions of manufacture and storage. The liquid
carrier or vehicle can be a solvent or liquid dispersion medium
comprising, for example, water, ethanol, a polyol (for example,
glycerol, propylene glycol, liquid polyethylene glycols, and the
like), vegetable oils, nontoxic glyceryl esters, and suitable
mixtures thereof. The proper fluidity can be maintained, for
example, by the formation of liposomes, by the maintenance of the
required particle size in the case of dispersions or by the use of
surfactants. The prevention of the action of microorganisms can be
brought about by various antibacterial and antifungal agents, for
example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal,
and the like. In many cases, it will be preferable to include
isotonic agents, for example, sugars, buffers or sodium chloride.
Prolonged absorption of the injectable compositions can be brought
about by the use in the compositions of agents delaying absorption,
for example, aluminum monostearate and gelatin.
Sterile injectable solutions are prepared by incorporating the
active compound in the required amount in the appropriate solvent
with various of the other ingredients enumerated above, as
required, followed by filter sterilization. In the case of sterile
powders for the preparation of sterile injectable solutions, the
preferred methods of preparation are vacuum drying and the freeze
drying techniques, which yield a powder of the active ingredient
plus any additional desired ingredient present in the previously
sterile-filtered solutions.
For topical administration, the present compounds may be applied in
pure form, i.e., when they are liquids. However, it will generally
be desirable to administer them to the skin as compositions or
formulations, in combination with a dermatologically acceptable
carrier, which may be a solid or a liquid.
Useful solid carriers include finely divided solids such as talc,
clay, microcrystalline cellulose, silica, alumina and the like.
Useful liquid carriers include water, alcohols or glycols or
water-alcohol/glycol blends, in which the present compounds can be
dissolved or dispersed at effective levels, optionally with the aid
of non-toxic surfactants. Adjuvants such as fragrances and
additional antimicrobial agents can be added to optimize the
properties for a given use. The resultant liquid compositions can
be applied from absorbent pads, used to impregnate bandages and
other dressings, or sprayed onto the affected area using pump-type
or aerosol sprayers.
Thickeners such as synthetic polymers, fatty acids, fatty acid
salts and esters, fatty alcohols, modified celluloses or modified
mineral materials can also be employed with liquid carriers to form
spreadable pastes, gels, ointments, soaps, and the like, for
application directly to the skin of the user.
Examples of useful dermatological compositions which can be used to
deliver the compounds of formula I to the skin are disclosed in
Jacquet et al. (U.S. Pat. No. 4,608,392), Geria (U.S. Pat. No.
4,992,478), Smith et al. (U.S. Pat. No. 4,559,157) and Wortzman
(U.S. Pat. No. 4,820,508).
Useful dosages of the compounds of formula I can be determined by
comparing their in vitro activity, and in vivo activity in animal
models. Methods for the extrapolation of effective dosages in mice,
and other animals, to humans are known to the art; for example, see
U.S. Pat. No. 4,938,949. Useful dosages of Type IV PDE inhibitors
are known to the art. For example, see, U.S. Pat. No. 5,877,180,
Col. 12.
Generally, the concentration of the compound(s) of formula (I) in a
liquid composition, such as a lotion, will be from about 0.1 25%
wt-%, preferably from about 0.5 10 wt-%. The concentration in a
semi-solid or solid composition such as a gel or a powder will be
about 0.1 5 wt-%, preferably about 0.5 2.5 wt-%.
The amount of the compound, or an active salt or derivative
thereof, required for use in treatment will vary not only with the
particular salt selected but also with the route of administration,
the nature of the condition being treated and the age and condition
of the patient and will be ultimately at the discretion of the
attendant physician or clinician.
In general, however, a suitable dose will be in the range of from
about 0.5 to about 100 .mu.g/kg, e.g., from about 10 to about 75
.mu.g/kg of body weight per day, such as 3 to about 50 .mu.g per
kilogram body weight of the recipient per day, preferably in the
range of 6 to 90 .mu.g/kg/day, most preferably in the range of 15
to 60 .mu.g/kg/day.
The compound is conveniently administered in unit dosage form; for
example, containing 5 to 1000 .mu.g, conveniently 10 to 750 .mu.g,
most conveniently, 50 to 500 .mu.g of active ingredient per unit
dosage form.
Ideally, the active ingredient should be administered to achieve
peak plasma concentrations of the active compound of from about 0.1
to about 10 nM, preferably, about 0.2 to 10 nM, most preferably,
about 0.5 to about 5 nM. This may be achieved, for example, by the
intravenous injection of a 0.05 to 5% solution of the active
ingredient, optionally in saline, or orally administered as a bolus
containing about 1 100 .mu.g of the active ingredient. Desirable
blood levels may be maintained by continuous infusion to provide
about 0.01 5.0 .mu.g/kg/hr or by intermittent infusions containing
about 0.4 15 .mu.g/kg of the active ingredient(s).
The desired dose may conveniently be presented in a single dose or
as divided doses administered at appropriate intervals, for
example, as two, three, four or more sub-doses per day. The
sub-dose itself may be further divided, e.g., into a number of
discrete loosely spaced administrations; such as multiple
inhalations from an insufflator or by application of a plurality of
drops into the eye. For example, it is desirable to administer the
present compositions intravenously over an extended period of time
following the insult that gives rise to inflammation.
The ability of a given compound of the invention to act as an
A.sub.2A adenosine receptor agonist (or antagonist) may be
determined using pharmacological models which are well known to the
art, or using tests described below.
The invention will be further described by reference to the
following detailed examples, which are given for illustration of
the invention, and are not intended to be limiting thereof.
EXAMPLE 1
Trans-(1-[4-hydroxymethyl)cyclohexyl]methyl)-4-methylbenzenesulfonate
(5.2). Sodium hydride (1.68 g, 70 mmol) was added to a solution of
10 g (70 mmol) [4-(hydroxymethyl)cyclohexyl]methan-1-ol (5.1) in
700 mL of tetrahydrofuran and stirred for 1 hour p-toluenesulfonyl
chloride (13.3 g, 70 mmol) was then added and the reaction mixture
was refluxed for 5 hours. The reaction was then cooled to 0.degree.
C. and slowly quenched with water until there is no more reactive
hydride. Once the hydride was quenched, the reaction mixture was
diluted with ether (700 mL) and extracted 2 times with 10% aqueous
potassium carbonate (700 mL). The organics were dried using sodium
sulfate and the solvent was removed under reduced pressure. The
product was purified by chromatography on silica gel column eluting
with acetone-dichloromethane (5:95) to give 5.2 (35%). .sup.1H NMR
(300 MHz, CDCl.sub.3) .delta. 7.75 (d, J=8.3 Hz, 2H), 7.32 (d,
J=8.1 Hz, 2H), 3.79 (d, J=6.35 Hz, 2H), 3.39 (d, J=6.35 Hz, 2H),
2.42 (s, 3H), 1.75 (m, 4H), 1.59 (m, 1H), 1.37 (m, 1H), 0.9 (m,
4H). .sup.13C NMR (300 MHz, CDCl.sub.3) .delta.145.3, 133.4, 130.3,
130.3, 128.3, 128.3, 75.8, 68.5, 40.6, 37.8, 28.9, 28.9, 28.9,
28.9, 22.1.
EXAMPLE 2
(4-prop-2-ynylcyclohexyl)methan-1-ol (5.3). Lithium acetylide
ethylenediamine complex (90%) (6.4 g, 70 mmol) was added very
slowly to a solution of 5.2 (3 g, 10 mmol) in 40 mL of dimethyl
sulfoxide. The reaction mixture was allowed to stir for 5 days and
then slowly quenched at 0.degree. C. with water. This mixture was
diluted with ether (300 mL) and extracted 3 times with saturated
aqueous ammonium chloride (200 mL). The organics were dried with
sodium sulfate. The solvent was removed under reduced pressure and
the product was purified by chromatography on silica gel column
eluting with ethyl acetate-hexanes (20:80) to give 5.3 (85%).
.sup.1H NMR (300 MHz, CDCl.sub.3) .delta.3.41 (d, J=6.5 Hz, 2H),
2.07 (dd, J=2.5, 6.5 Hz, 2H), 1.96 1.75 (m, 5H), 1.41 (m, 2H),
0.095 (m, 4). .sup.13C NMR (300 MHz, CDCl.sub.3) .delta. 83.8,
69.6, 68.9, 40.7, 37.7, 32.3, 32.3, 29.6, 29.6, 26.5.
EXAMPLE 3
4-prop-2-ynylcyclohexanecarboxylic acid (5.4). A solution of
chromium trioxide (1.1 g, 11 mmol) in 1.5 M sulfuric acid (40 mL,
27 mmol) was maintained at 0.degree. C. while 5.3 (0.46 g, 3 mmol)
in 80 mL of acetone was added over 2 hours. The reaction was then
stirred for an additional 2 hours at room temperature. The reaction
mixture was diluted with ether (200 mL) and extracted 2 times with
water. The organics were dried with sodium sulfate. The solvent was
removed under reduced pressure and the product was purified by
chromatography on silica gel column eluting with
acetone-dichloromethane (70:30) to give 5.4 (75%). .sup.1H NMR (300
MHz, CDCl.sub.3) .delta. 2.24 (dt, J=3.66, 12.1 Hz, 1H), 2.10 (dd,
J=2.7, 6.5 Hz, 2H), 2.04 1.89 (m, 5H), 1.76 (d, J=2.3 Hz, 1H), 1.43
(dq, J=3.28, 13.1 Hz, 2H), 1.03 (dq, J=3.28, 13.1 Hz, 2H). .sup.13C
NMR (300 MHz, CDCl.sub.3) .delta.183.2, 83.3, 69.9, 43.4, 36.7,
31.8, 28.9, 26.3.
EXAMPLE 4
Methyl 4-prop-2-ynylcyclohexanecarboxylate (5.5)
(Trimethylsilyl)diazomethane (2.0 M) solution in hexanes (1 mL, 2
mmol) was added to a solution of 5.4 (0.34 g, 2 mmol) in 15 mL of
methanol:dichloromethane (3:7). The solvents were removed under
reduced pressure resulting in 100% conversion of starting material
to product. .sup.1H NMR (300 MHz, CDCl.sub.3) .delta. 2.24 (dt,
J=3.66, 12.1 Hz, 1H), 2.10 (dd, J=2.7, 6.5 Hz, 2H), 2.06 (dd,
J=1.54, 6.54 Hz, 1H), 2.00 1.89 (m, 3H), 1.76 (d, J=2.3 Hz, 1H),
1.43 (dq, J=3.28, 13.1 Hz, 2H), 1.03 (dq, J=3.28, 13.1 Hz, 2H).
.sup.13C NMR (300 MHz, CDCl.sub.3) .delta. 176.8, 83.3, 69.8, 51.9,
43.4, 36.7, 31.9, 29.2, 26.3.
EXAMPLE 5
[(2R,3R,4R,5R)-3,4-diacetyloxy-5-(2-amino-6-oxohyropurin-9-yl)oxolan-2-yl-
]methyl acetate (6.2). A suspension of 113 g (0.4 mol) of dry
guanosine (6.1), acetic anhydride (240 mL, 2.5 mol), dry pyridine
(120 mL) and dry DMF (320 mL) was heated for 3.75 hours at
75.degree. C. without allowing the temperature to exceed 80.degree.
C. The clear solution was then transferred to a 3 L Erlenmeyer
flask and filled with 2-propanol. Upon cooling the solution to room
temperature crystallization was initiated and allowed to proceed at
4.degree. C. overnight. The white solid filtrate was filtered,
washed with 2-propanol and recrystallized from 2-propanol to give
6.2 (96%). .sup.1H NMR (300 Mhz, CDCl.sub.3) .delta. 8.20 (s, 1H,
H-8), 6.17 (d, J=5.41 Hz, 1H, H-1') 5.75 (t, J=5.39 Hz, 1H, H-2'),
5.56 (t, J=5.0, H-3'), 4.41 (m, 3H, H-4',5'), 2.14 (s, 3H, Ac),
2.11 (s, 3H, Ac), 2.10 (s, 3H, Ac). .sup.13C NMR (300 MHz,
CD.sub.3OD) .delta. 171.0, 170.3, 1702, 157.7, 154.8, 152.4, 136.7,
117.7, 85.5, 80.4, 73.0, 71.3, 64.0, 31.3, 21.2, 21.0.
EXAMPLE 6
[(2R,3R,4R,5R)-3,4-diacetyloxy-5-(2-amino-6-chloropurin-9-yl)oxolan-2-yl]-
methyl acetate (6.3). To a 1000 mL flask was added 80 g (0.195 mol)
[(2R,3R,4R,5R)-3-4-diacetyloxy-5-(2-amino-6-oxohyropurin-9-yl)oxolan-2-yl-
]methyl acetate (6.2), tetramethylammonium chloride (44 g, 0.4
mol), anhydrous acetonitrile (400 mL) and N,N-dimethylaniline (25
mL). The flask was placed in an ice salt bath and cooled to
2.degree. C. To this solution was added dropwise POCl.sub.3 (107 mL
1.15 mol) at a rate that maintained the temperature below 5.degree.
C. (45 minutes). The flask was then removed from the ice bath,
outfitted with a condenser, placed in an oil bath and allowed to
reflux for 10 minutes whereas the solution changed to a red/brown
color. The solvent was then removed under reduced pressure to yield
an oily residue which was transferred to a beaker containing 1000 g
of ice and 400 mL of CHCl.sub.3 and allowed to stir for 1.5 hours
to decompose any remaining POCl.sub.3. The organic phase was then
removed and the aqueous phase extracted with 3.times.50 mL of
CHCl.sub.3 and pooled with the organic phase. The pooled organic
was then back extracted with 50 mL of water followed by stirring
with 200 mL of saturated NaHCO.sub.3. The organic was further
extracted with NaHCO.sub.3 until the aqueous extract was neutral
(2.times.). The organic was finally extracted with brine and then
dried over MgSO.sub.4 for 16 hours. To the solution was added 800
mL of 2-propanol after which the solution was concentrated under
reduced pressure. To the oily solid was added 200 mL of 2-propanol
and the solution was refrigerated overnight. The crystalline
product was filtered, washed, and allowed to dry overnight to give
6.3 (77%). .sup.1H NMR (300 MHz, CD.sub.3OD) .delta. 8.31 (s, 1H,
H-8), 7.00 (s, 2H, NH.sub.2) 6.06 (d, J=5.8 Hz, 1H, H-1'), 5.83 (t,
J=6.16 Hz, 1H, H-2'), 5.67 (m, 1H, H-3'), 4.29 (m, 3H, H-4',5'),
2.07 (s, 3H, Ac), 1.99 (s, 3H, Ac), 1.98 (s, 3H, Ac). .sup.13C NMR
(300 MHz, CD.sub.3OD) .delta. 171.0, 170.4, 170.2, 160.8, 154.6,
150.8, 142.2, 124.5, 85.8, 80.6, 72.8, 71.2, 63.9, 21.4, 21.3,
21.1.
EXAMPLE 7
[(2R,3R,4R,5R)-3,4-diacetyloxy-5-(6-chloro-2-iodopurin-9-yl)oxolan-2-yl]m-
ethyl acetate (6.4). Isoamyl nitrite (5 mL, 37 mmol) was added to a
mixture of 5.12 g (12 mmol)
[(2R,3R,4R,5R)-3-,4-diacetyloxy-5-(2-amino-6-chloropurin-9-yl)oxolan-2-yl-
]methyl acetate (6.3), I.sub.2 (3.04 g, 12 mmol), CH.sub.2I.sub.2
(10 mL, 124 mmol), and CuI (2.4 g, 12.6 mmol) in THF (60 mL). The
mixture was heated under reflux for 45 minutes and then allowed to
cool to room temperature. To this solution was added 100 ml of sat.
Na.sub.2S.sub.2O.sub.3 which removed the reddish color due to
iodine. The aqueous was extracted 3.times. with chloroform, which
was pooled, dried over MgSO.sub.4, and concentrated under reduced
pressure. The product was then purified over a silica gel column
using CHCl.sub.3--MeOH (98:2) to collect
[(2R,3R,4R,5R)-3,4-diacetyloxy-5-(6-chloro-2-iodopurin-9-yl)oxola-
n-2-yl]methyl acetate (6.4) (80% crystallized from EtOH). .sup.1H
NMR (300 MHz, CDCl.sub.3) .delta. 8.20 (s, 1H H-8), 6.17 (d, J=5.41
Hz, 1H, H-1'), 5.75 (t, J=5.39 Hz, 1H, H-2'), 5.56 (t, J=5.40 Hz,
1H, H-3'), 4.38 (m, 3H, H-4',5'), 2.14 (s, 1H, Ac), 2.11 (s, 1H,
Ac), 2.10 (s, 1H, Ac).
EXAMPLE 8
(4S,2R,3R,5R)-2-(6-amino-2-iodopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-d-
iol (6.5). To a flask containing 6.0 g (11.1 mmol)
[(2R,3R,4R,5R)-3,4-diacetyloxy-5-(6-chloro-2-iodopurin-9-yl)oxolan-2-yl]m-
ethyl acetate (6.4) was added 100 ml of liquid NH.sub.3 at
-78.degree. C. and the solution was allowed to stir for 6 hours.
After which time it was allowed to come to r.t. overnight with
concurrent evaporation of the NH.sub.3 to yield a brown oil. The
product was crystallized from hot isopropanol to give 6.5 (80%),
m.p. 143 145.degree. C., r.f.=0.6 in 20% MeOH/CHCl.sub.3. .sup.1H
NMR (300 MHz, DMSO-d.sub.6) .delta. 8.24 (s, 1H), 7.68 (s, 2H),
5.75 (d, J=6.16, 1H), 5.42 (d, J=5.40 Hz, 1H), 5.16 (d, J=4.62 Hz,
1H), 4.99 (t, J=5.39 Hz, 1H), 4.67 (d, J=4.81 Hz, 1H), 4.06 (d,
J=3.37 Hz, 1H), 3.89 (m, 1H), 3.54 (m, 2H).
EXAMPLE 9
[(1R,2R,4R,5R)-4-(6-amino-2-iodopurin-9-yl)-7-7-dimethyl-3,6,8-trioxabicy-
clo[3.3.0]oct-2-yl]methan-1-ol (6.6). To a solution of 2.0 g (5.08
mmol)
(4S,2R,3R,5R)-2-(6-amino-2-iodopurin-9-yl)-5(hydroxymethyl)oxolane-3,4-di-
ol (6.6) in 100 mL acetone was added 9.6 g of p-toluene-sulfonic
acid and 5 mL of dimethoxypropane. The reaction was stirred at room
temperature for 1 hour at which time 15 g of NaHCO.sub.3 and then
stirred for an additional 3 hours. the residue was filtered and
washed 2.times. with EtOAc. The filtrate was then concentrated
under reduced pressure. The residue was chromatographed on a silica
gel column with MeOH-CHCl.sub.3 (1:99) to give 6.6 (72%) as a
solid, m.p. 185 187.degree. C. .sup.1H NMR (300 MHz, DMSO-d.sub.6)
.delta. 8.22 (s, 1H, H-8), 7.69 (s, 2H), NH.sub.2), 6.00 (d, J=2.70
Hz, 1H, H-1'), 5.21 (m, 1H, H-2'), 5.07 (bs, 1H, OH), 4.88 (m, 1H,
H-3'), 4.13 (m, 1H, H-4'), 3.47 (m, 2H, H-5'), 1.49 and 128 (s, 3H,
C(CH.sub.3).sub.2).
EXAMPLE 10
(2S,1R,4R,5R)-4-(6-amino-2-iodopurin-9-yl)-7,7-dimethyl-3,6,8-trioxabicyc-
lo[3.3.0]octane-2-carboxylic acid (6.7). To a stirred solution of
1.6 g (3.7 mmol) of
[(1R,2R,4R,5R)-4-(6-amino-2-iodopurin-9-yl)-7-7-dimethyl-3,6,8-trioxabicy-
clo[3.3.0]oct-2-yl]methan-1-ol (6.6) in 200 mL of H.sub.2O was
added 0.60 g of KOH and, dropwise, a solution of 1.70 g (10.8 mmol)
of KMnO.sub.4 in 50 mL of H.sub.2O. The mixture was set aside in
the dark at room temperature for 225 hours. The reaction mixture
was then cooled to 5 10.degree. C. and decolorized by a solution of
4 mL of 30% H.sub.2O2 in 16 mL of water, while the temperature was
maintained under 10.degree. C. using an ice-salt bath. The mixture
was filtered through Celite and the filtrate was concentrated under
reduced pressure to about 10 mL and then acidified to pH 4 with 2N
HCl. The resulting precipitate was filtered off and washed with
ether to yield 6.7 (70%) after drying as a white solid, m.p. 187
190.degree. C. .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta. 8.11 (s,
1H, H-8), 7.62 (s, 2H, NH.sub.2), 7.46 (s, 1H, COOH), 6.22 (s, 1H,
H-1'), 5.42 (d, J=5.71 Hz, 1H, H-2'), 5.34 (d, J=6.16 Hz, 1H,
H-3'), 4.63 (s, 1H, H-4'), 1.46 and 1.30 (s, 3H,
C(CH.sub.3).sub.2).
EXAMPLE 11
(2S,3S,4R,5R)-5-(6-amino-2-iodopurin-9-yl)-3,4-dihydroxyoxolane-2-carboxy-
lic acid (6.8). A solution of 1.72 g (3.85 mmol) of
(2S,1R,4R,5R)-4-(6-amino-2-iodopurin-9-yl)-7,7-dimethyl-3,6,8-trioxabicyc-
lo[3.3.0]octane-2-carboxylic acid (6.7) in 80 mL of 50% HCOOH was
stirred at 80.degree. C. for 1.5 hours. The reaction mixture was
evaporated under reduced pressure, dissolved in H.sub.2O, and the
solution was evaporated again. This process was repeated until
there was no odor of formic acid in the residue. Recrystallization
from water yielded 1.33 g (85%) 6.8 as a white solid, m.p. 221
223.degree. C. dec. .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
8.31 (s, 1H, H-8), 7.68 (s, 2H, NH.sub.2), 5.90 (d, J=6.55 Hz, 1H,
H-1'), 4.42 (m, 1H, H-2'), 4.35 (d, J=2.31 Hz, 1H, H-4'), 4.22 (m,
1H, H-3').
EXAMPLE 12
[(2S,3S,4R,5R)-5-(6-amino-2-iodopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]-N-e-
thylcarboxamide (6.9). To a cooled (5.degree. C.) and stirred
solution of 1.29 g (3.17 mmol) of
(2S,3S,4R,5R)-5-(6-amino-2-iodopurin-9-yl)-3,4-dihydroxyoxolane-2-carboxy-
lic acid (6.8) in 150 mL of absolute ethanol was added dropwise
1.15 mL of ice-cooled SOCl.sub.2. The mixture was stirred at room
temperature overnight and then brought to pH 8 with saturated
aqueous NaHCO.sub.3. The mixture was filtered, and then the
filtrate was concentrated under reduced pressure to yield a white
solid which was dried and then redissolved in 20 mL of dry
ethylamine at -20.degree. C. for 3 hours and then at room
temperature overnight. The reaction mixture was diluted with
absolute ethanol, and the precipitated product was filtered off and
washed with dry ether to give 530 mg (72%) of 6.9 as a pure solid,
m.p. 232 234.degree. C. .sup.1H NMR (300 MHz, DMSO-d.sub.6) .delta.
8.34 (s, 1H, H-8), 8.12 (t, 1H, NH), 7.73 (s, 2H, NH.sub.2), 5.85,
(d, J=6.93 Hz, 1H, H-1), 4.54 (m, 1H, H-2'), 4.25 (d, J=1.92 Hz,
1H, H-4'), 4.13 (m, 1H, H-3'), 3.28 (m, 2H, CH.sub.2CH.sub.3), 1.00
(t, J=7.2 Hz, 3H, CH.sub.2CH.sub.3).
EXAMPLE 13
Methyl-4-(3-{9-[(4S,5S,2R,3R)-5-(N-ethylcarbamoyl)-3,4-dihydroxyoxolan-2--
yl-6-aminopurin-2-yl)}prop-2-ynyl)cyclohexane-carboxylate
(DWH-146e). To a degassed solution of 25 mg (0.063 mmol) of [(2S,3
S,4R,5R)-5-(6-amino-2-iodopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]-N-ethylca-
rboxamide (6.9), 16.9 mg (0.094 mmol) (5.5), and 0.75 mg CuI in 5
mL each of TEA and acetonitrile was added 15 mg of
Pd(PPh.sub.3).sub.4. The solution was stirred for 24 hours at
70.degree. C. after which time the solution was filtered through
celite and chromatographed on silica gel with MeOH-CHCl.sub.3
(5:95) to give DWH-146e (24%).
EXAMPLE 14
(4-prop-2-ynylcyclohexyl)methyl acetate (5.6). Acetic anhydride
(0.92 mL, 8.25 mmol) and pyridine (0.2 mL, 2.5 mmol) were added to
a solution of 5.3 (250 mg, 1.65 mmol) in 25 mL ether. The reaction
was allowed to stir at ambient temperature for 24 hours. Water was
added to the reaction and the organic was further extracted with
10% NaHCO.sub.3. The organic layer was dried with MgSO.sub.4 and
evaporated. The residue was chromatographed on silica gel with
EtOAc-Hexanes (5:95) to yield 5.6 (47%).
EXAMPLE 15
[4-(3-{9-(4S,5S,2R,3R)-5-(N-ethylcarbamoyl)-3,4-dihydroxyoxolan-2-yl]-6-a-
minopurin-2-yl}prop-2-ynyl)cyclohexyl]methyl acetate (JMR193). To a
degassed solution of 125 mg (0.29 mmol) of
[(2S,3S,4R,5R)-5-(6-amino-2-iodopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]-N-e-
thylcarboxamide (6.9), 150 mg (0.77 mmol) (5.6), and 1.0 mg CuI in
1.3 mL of TEA and 4 mL DMF was added to 25 mg of
Pd(PPh.sub.3).sub.4. The solution was stirred for 72 hours at
60.degree. C. after which time the solution was filtered through
celite and chromatographed on silica gel with MeOH-CHCl.sub.3
(5:95) to give JMR193 (10%).
EXAMPLE 16
Radioligand Binding Studies. Binding to A.sub.2A receptors was
evaluated with the radioligand .sup.125I-ZM241385. FIG. 1 depicts
the competition by selective agonists for binding to recombinant
human A.sub.2A adenosine receptors. DWH-146e is highly selective
for the recombinant human A.sub.2A (hA2A) subtype. Selectivity for
the A.sub.3 receptor (not shown) is less impressive, but still
about 50-fold. DWH-146e is about 5 and 50 times more potent than
WRC0470 and CGS21680, respectively (FIG. 1). An unexpected and
interesting finding is that the ester, DWH-146e also is about 50
times more potent than the acid, DWH-146a (FIG. 1).
EXAMPLE 17
Effect of DWH-146e and JMR193 on Neutrophil Oxidative Activity
A. Materials.
f-met-leu-phe (fMLP), luminol, superoxide dismutase, cytochrome C,
fibrinogen, adenosine deaminase, and trypan blue were obtained from
Sigma Chemical. Ficoll-hypaque was purchased from ICN (Aurora,
Ohio), and Cardinal Scientific (Santa Fe, N.Mex.) and Accurate
Chemicals and Scientific (Westerbury, N.Y.). endotoxin
(lipopolysaccharide; E. coli K235) was from List Biologicals
(Campbell, Calif.). Hanks balanced salt solution (HBSS), and
limulus amebocyte lysate assay kit were from BioWittaker
(Walkersville, Md.). Human serum albumin (HSA) was from Cutter
Biological (Elkhart, Ind.). Recombinant human tumor necrosis
factor-alpha was supplied by Dianippon Pharmaceutical Co. Ltd.
(Osaka, Japan). ZM241385
(4-(2-[7-amino-2-(2-furyl)-[1,2,4]-triazolo[2,3-a][1,3,5]triazin-5-yl
amino]ethyl)phenol) was a gift from Simon Poucher, Zeneca
Pharmaceuticals, Cheshire, UK. Stock solutions (1 mM and 10 mM in
DMSO) were made and stored at -20.degree. C.
B. Human Neutrophil Preparation
Purified neutrophils (.about.98% neutrophils and >95% viable by
trypan blue exclusion) containing <1 platelet per 5 neutrophils
and <50 pg/mL endotoxin (limulus amebocyte lysate assay) were
obtained from normal heparinized (10 U/mL) venous blood by a one
step Ficoll-hypaque separation procedure (A. Ferrante et al., J.
Immunol. Meth., 36, 109 (1980)).
C. Release of Inflammatory Reactive Oxygen Species from Primed and
Stimulated Human Neutrophils Chemiluminescence
Luminol-enhanced chemiluminescence, a measure of neutrophil
oxidative activity, is dependent upon both superoxide production
and mobilization of the lysosomal granule enzyme myeloperoxidase.
The light is emitted from unstable high-energy oxygen species
generated by activated neutrophils. Purified neutrophils (5
10.times.10.sup.5/ml) were incubated in Hanks balanced salt
solution containing 0.1% human serum albumin (1 ml) with or without
DWH-146a, DWH-146e, CGS21680, or JMR193 with or without rolipram
and with or without tumor necrosis factor-alpha (1 U/ml) for 30
minutes at 37.degree. C. in a shaking water bath. Then luminol
(1.times.10.sup.-4 M) enhanced f-met-leu-phe (1 mcM) stimulated
chemiluminescence was read with a Chronolog.RTM. Photometer
(Crono-log Corp., Havertown, Pa.) at 37.degree. C. for 2 4 minutes.
Chemiluminescence is reported as relative peak light emitted
(=height of the curve) compared to samples with tumor necrosis
factor-alpha and without DWH, JMR or rolipram.
D. Results
As shown in FIG. 2, JMR193 and DWH-146e both decreased tumor
necrosis factor-alpha-primed f-met-leu-phe-stimulated human
neutrophil oxidative activity as measured by luminol-enhanced
chemiluminescence more effectively than the adenosine A.sub.2A
receptor agonist CGS21680. The horizonal axis gives the
concentration of CGS21680, DWH-146a, DWH-146e or JMR193 (log nM).
The vertical axis gives the resulting peak human neutrophil
activity as relative amount of stimulated release of reactive
oxygen species as measured with luminol-enhanced chemiluminescence
compared to control samples which were not primed with tumor
necrosis factor-alpha. Means SEM (n=4 5 separate experiments).
The data below the horizontal axis in FIG. 2 gives the EC.sub.50
for reducing the human neutrophil activity (based on the data in
FIG. 2). Means SEM (n=4 5 separate experiments). *p<0.05
decreased IC.sub.50 compared to CGS21680.
JMR193 and DWH-146e decreased the stimulated-neutrophil oxidative
burst with EC.sub.50's less than 1 nM (0.8 and 0.3 nM,
respectively). In contrast, the free acid A.sub.2A adenosine
receptor agonists DWH-146a and CGS21680 were not as effective in
inhibiting the oxidative burst (53 and 9 nM, respectively; FIG. 2).
DWH-146e inhibition of the stimulated neutrophil oxidative burst
was antagonized by the selective A.sub.2A AR antagonist
ZM241385.
As shown in FIG. 3, JMR193 (1 nM) with rolipram (100 nM)
synergistically decreased stimulated release of reactive oxygen
species. Human neutrophils were primed with tumor necrosis
factor-alpha (1 U/ml) and stimulated with f-met-leu-phe (1 .mu.M).
The vertical axis gives the percent inhibition of oxidative
activity as measured by luminol-enhanced chemiluminescence. Means
SEM (n=4 separate experiments. *p<0.05 synergy between JMR193
and rolipram compared to additive activity.
As shown in FIG. 4, the highly selective A.sub.2A adenosine
receptor antagonist ZM241385 (100 nM) (ZM) counteracted human
neutrophil oxidative activity inhibited by JMR193 (10 nM) as
measured by luminol-enhanced chemiluminescence. Means SEM of 4
separate experiments. *p=0.0004 ZM241385 counteracted JMR193
inhibited oxidative activity.
E. Human Neutrophil [cAMP].sub.i and Neutrophil Adherence to a
Biological Surface
A 24 well tissue culture plate was coated with human fibrinogen (5
mg/ml in 1.5% sodium bicarbonate; 0.5 ml/well; Sigma Chemical)
overnight at 37.degree. C. in 5% CO.sub.2. Neutrophils (3
4.times.10.sup.6/0.5 ml/sample) were incubated within a well of the
coated plate for 45 minutes in 0.5 ml of HBSS containing 0.1% HSA
and ADA (1 U/ml) in the presence and absence of recombinant human
TNF.alpha. (10 U/ml), DWH-146e (3 300 nM), rolipram (300 nM),
and/or ZM241385 (100 nM). Following incubation, 0.5 ml HCl (0.2 N)
was added to the wells and incubated for 45 minutes more at room
temperature to extract the cAMP. The samples were then centrifuged
in a microfuge for 2 minutes to remove cell debris. Half ml samples
were frozen for cAMP analyses (B. Brooker et al., Science, 194, 270
(1976)). The wells were washed twice with normal saline and the
remaining monolayer digested with 0.2 ml of 0.2 N NaOH containing
SDS for 2 hours at room temperature. The protein samples were then
frozen (-70.degree. C.) for later protein analysis to determine
relative PMN adherence (K. P. Stowell et al., Anal. Biochem., 85,
572 (1978)).
Results
DWH-146e (30 300 nM) alone and synergistically with rolipram (300
nM) increased human neutrophil cAMP content and with rolipram
synergistically decreased neutrophil adherence to a
fibrinogen-coated surface (FIG. 5). The effects of DWH-146e (300
nM)+rolipram (300 nM) on neutrophil cAMP production and adherence
were counteracted by the selective A.sub.2A adenosine receptor
antagonist, ZM241385 (ZM; 100 nM). Mean SEM of 5 separate
experiments. *p<0.05 increased neutrophil [cAMP] compared to
without DWH-146e; **p<0.05 decreased neutrophil adherence
compared to no DWH-146e.
F. Adherent Human Neutrophil Oxidative Activity
Methods. Using methods modified from Section E, neutrophils
(2.times.10.sup.6/ml) from Ficoll-Hypaque separation were incubated
15 minutes 37.degree. C. in 0.45 ml of Hanks balanced salt solution
containing 0.1% human serum albumin and adenosine deaminase (1
U/ml), rolipram (300 nM), and DWH-146e (3 300 nM). Following
incubation, cytochrome C (120 .mu.M) and catalase (0.062 mg/ml) are
added in the presence and absence of recombinant human tumor
necrosis factor-alpha (1 U/ml) and 200 .mu.l aliquots of cell
suspension were immediately transferred to duplicate wells of a 96
well flat-bottomed tissue culture plate which had been coated
overnight with human fibrinogen. The optical density of the samples
were read at 550 nm against matched superoxide dismutase (200 U/ml)
samples.
G. Results
As shown in FIG. 6, inhibition of tumor necrosis factor-alpha
(TNF)-stimulated adherent human neutrophil superoxide release on a
fibrinogen-coated surface was accomplished by rolipram (300 nM) and
DWH-146e. DWH-146e decreased the oxidative burst of adhering
neutrophils, and synergistically decreased the oxidative burst in
the presence of rolipram, which by itself did not affect neutrophil
oxidative activity. The horizontal axis gives the DWH-146e
concentration in nM and the vertical axis gives the amount of
superoxide released by the neutrophils as measured by cytochrome c
reduction. There was marked synergy with DWH-146e and the type IV
PDE inhibitor, rolipram, to decrease tumor necrosis
factor-alpha-stimulated adherent human neutrophil oxidative
activity. Means SEM of replicates from 4 5 separate experiments.
*p<0.05 decreased superoxide release compared to without
DWH-146e; **p<0.05 decreased superoxide release compared to with
rolipram and without DWH-146e.
EXAMPLE 18
Treatment of Ischemia/Reperfusion (I/R) Injury in Kidney with
DWH-146e
To determine whether or not DWH-146e induced A.sub.2A adenosine
receptor activation reduces plasma creatinine at 24 and 48 hours
following I/R injury in rats, rat kidneys were subjected to 45
minutes ischemia and 24 or 48 hours of reperfusion. DWH-146e (0.004
.mu.g/kg/min) or vehicle was administered continuously via minipump
beginning 5 hours prior to I/R. As shown in FIG. 7, DWH-146e
significantly decreased plasma creatinine in 7/7 rats (P<0.05)
and in 6/6 rats treated with DWH-146e (P<0.001), at 24 and 48
hours, respectively.
To determine whether or not the effect of DWH-146e on reduction of
plasma creatinine in rats subjected to I/R is A.sub.2A-receptor
mediated, rat kidneys were subjected to 45 minutes ischemia
followed by 48 hours reperfusion. DWH-146e (0.004 .mu.g/kg/min) was
administered continuously via minipump beginning 5 hours prior
ischemia. As shown in FIG. 8, the improvement in renal function was
reversed by the A.sub.2A antagonist ZM-241385 (0.003
.mu.g/kg/min-equimolar delivery rate compared with DWH-146e)
(*P<0.001 for Vehicle vs. DWH; **P<0.05 DWH vs. DWH/ZM. N=5
for Vehicle, DW; N=6 for DWH/ZM. ANOVA followed by Bonferroni
correction).
DWH-146e, at concentrations that have no hemodynamic effects,
prevents renal edema, necrosis and red cell pooling in the inner
medulla.
The protection against renal damage afforded by DWH-146e (0.01
.mu.g/kg/min s.c. for 48 hours) was correlated with a dramatic
inhibition of neutrophil adherence to vascular endothelium. It is
believed that inhibition by DWH-146e of the interaction between
neutrophils and vascular endothelium is responsible, at least in
part, for the protection against renal damage.
To determine whether or not A.sub.2A-AR activation reduces
neutrophils in the outer medulla of rats subjected to I/R, using
Neurolucida.RTM., the kidney was viewed under 100.times. mag and
the entire kidney was drawn. PMNs were counted by viewing kidney
sections under 250.times. mag. Kidney sections were overlaid with
optical frames viewed under the microscope and all PMNs were
counted within each frame. This system prevents counting of PMNs
more than once. As shown in FIG. 9, the density of neutrophils was
15.65/mm.sup.2 for vehicle and 3.02/mm.sup.2 for DWH-146e
treatment.
EXAMPLE 19
Effect of DWH-146e on Lung Reperfusion Injury
A. Methods. An isolated, whole blood-perfused, ventilated rabbit
lung model was used. Donor rabbits underwent lung harvest after
pulmonary arterial PGE.sub.1 injection and Euro-Collins
preservation solution flush, and lungs were preserved for 18 hours
at 4.degree. C. Group I lungs (n=9) served as control subjects.
Group II lungs (n=9) were reperfused with whole blood that was
first passed through a leukocyte-depleting filter. In group III
(n=9), DWH-146e was added to the blood reperfusate (25 .mu.g/kg)
immediately before reperfusion and was administered throughout the
reperfusion period (1 .mu.g/kg/min). All lungs were reperfused for
30 minutes, and pulmonary artery pressure (PAP), pulmonary vascular
resistance (PVR), airway compliance (CPL) and arterial oxygenation
were recorded. Mycloperoxidase activity (MPO) was recorded to
quantify neutrophil sequestration, and wet/dry weight ratios were
measured to demonstrate pulmonary edema.
B. Results. Arterial oxygentation in group II and group III was
significantly higher than that of group I after 30 minutes of
reperfusion (514.27.+-.35.80 and 461.12.+-.43.77 vs. 91.41.+-.20.58
mm Hg, p<0.001. As shown in FIG. 10, group III lungs displayed a
progressive involvement in pO.sub.2 throughout reperfusion.
Leukocyte depletion in group II lungs improved arterial oxygenation
in early reperfusion. *p=0.004 (group II versus groups I and III);
**p<0.001 (groups II and III versus group I).
As shown in FIG. 11, mean PVR in group II was significantly reduced
when compared to controlled lungs (*p<0.001). PVR of group III
lungs was significantly lower than even those lungs that underwent
reperfusion with leukocyte-depleted blood (**p<0.001 versus
groups I and II). Pulmonary vascular resistance was significantly
reduced in group III (22,783.+-.357 dynesscm.sup.-5) compared to
both group II and group I (31,057.+-.1743 and 36,911.+-.2173
dynesscm.sup.-5, p<0.001). Airway compliance was improved in
groups II and III when compared to group I (1.68.+-.0.08 and
1.68.+-.0.05 vs. 1.36.+-.0.13, p=0.03). Microvascular permeability
in group III was reduced to 106.82.+-.17.09 compared with
165.70.+-.21.83 ng Evans-blue dye/gm tissue in group I (p=0.05). As
shown in FIG. 12, myeloperoxidase activity in group III was
significantly lower than in group I (*p=0.03). MPO=myeloperoxidase.
Group III myeloperoxidase activity was 39.88.+-.4.87 compared with
88.70.+-.18.69 .DELTA.OD/gm/min in group I (p=0.03), and group II
myeloperoxidase activity was 56.06.+-.7.46.
C. Conclusions. DWH-146e reduced lung neutrophil sequestration and
dramatically improved pulmonary graft function. Neutrophils are
important components of the inflammatory cascade of reperfusion
injury and their source may include both the circulating blood and
the lung graft itself. Selective adenosine-A.sub.2A activation
interrupts the neutrophil-mediated inflammatory response and
reduces lung reperfusion injury following transplantation.
Under light microscopy, control lungs in group I showed severe
leukocyte infiltration and edema formation in the alveolar spaces
after 18 hours of ischemic storage and 30 minutes of reperfusion.
In group II, lungs that underwent reperfusion with
leukocyte-depleted blood and in group III lungs (that received
DWH-146e during reperfusion, this infiltration was much less.
EXAMPLE 20
Effect of DWH-146e on Neointimal Formation after Arterial Injury.
Leukocyte activation with release of inflammatory cytokines occurs
after percutaneous coronary intervention and may play a role in
restenosis. In the mouse, robust neointima formation in the
presence of an intact endothelial lining occurs after ligation of
the common carotid artery. Using this model, C57/BL6 mice were
randomized at the time of carotid ligation to a 7 day infusion via
osmotic pump of DWH-146e, (n=7), or vehicle (n=8).
At 14 days after carotid ligation, histomorphometry demonstrated a
significant reduction in neointimal area (0.005.+-.0.004 mm.sup.2
vs. 0.021.+-.0.014 mm.sup.2, p=0.02) and neointimal to medial area
ratio (0.13.+-.0.07 vs. 0.64.+-.0.44, p=0.01) in the treated
animals compared to controls. Medial area was similar in the two
groups (0.034.+-.0.007 mm.sup.2 vs. 0.036.+-.0.009 mm.sup.2,
p=0.81). This benefit in limiting neointimal growth persisted to 28
days. FIG. 13 summarizes the effect of DWH-146e to inhibit
neointimal growth in the mouse LCCA model. These experiments
demonstrate that, in a mouse carotid artery ligation model,
prolonged A.sub.2A stimulation (7 days) by DWH-146e results in a
significant reduction in neointimal formation for at least 21 days,
possibly through its effect on leukocyte activation and
function.
EXAMPLE 21
Inhibition of Endotoxin-Stimulated Human Monocyte TNF.alpha.
Release
A. Materials.
Ficoll-hypaque was purchased from ICN (Aurora, Ohio) and Cardinal
Scientific (Santa Fe, N.Mex.) and Accurate Chemicals and Scientific
(Westbury, N.Y.). Endotoxin (lipopolysaccharide; E. coli 0111B4)
was from List Biologicals (Campbell, Calif). Hanks balanced salt
solution (HBSS), and limulus amebocyte lysate assay kit were from
BioWittaker (Walkersville, Md.). Human serum albumin (HSA) was from
Cutter Biological (Elkhart, Ind.). ZM241385
(4-(2-[7-amino-2-(2-furyl)[1,2,4]-triazolo[2,3-a][1,3,5]triazin-5-yl
amino]ethyl)phenol) was a gift from Simon Poucher, Zeneca
Pharmaceuticals, Cheshire, UK. Stock solutions (1 mM and 10 mM in
DMSO) were made and stored at -20.degree. C.
B. Production of TNF.alpha. by Purified Human Adherent
Monocytes.
Methods: A monocyte rich monolayer (>65% monocytes) was prepared
by incubating 1 ml of the mononuclear leukocyte fraction
(5.times.10.sup.5/ml) from a Ficoll-Hypaque separation (A. Ferrante
et al., J. Immunol. Meth., 36, 109 (1980)) in wells of a 24 well
tissue culture plate (1 hr; 37.degree. C.; 5% CO.sub.2). The
non-adherent leukocytes were removed by washing and culture medium
(1 ml Hanks balanced salt solution, containing 0.1% human serum
albumin, adenosine deaminase [5 U/ml] and 1% heat-inactivated
autologous serum) added to the wells containing the adherent
mononuclear cells. As stated, the following were added: (1)
endotoxin (100 ng/ml) and the A.sub.2A AR selective antagonist
ZM241385 (100 nM) and, (2) A.sub.2A adenosine receptor selective
agonists JMR193 (1 1000 nM), DWH146e (1 1000 nM) and CGS21680 (10
1000 nM). The samples were then incubated for 4 hours (37.degree.
C.; 5% CO.sub.2) and the supernatants harvested. Any suspended
cells were removed by centrifugation and the cell-free samples
frozen (-70.degree. C.). TNF.alpha. was assayed in the cell-free
supernatants (n=6) by an ELISA kit (Coulter/Immunotech, Miami,
Fla.).
C. Results.
As shown in FIG. 14, the A.sub.2A adenosine receptor agonists
decreased endotoxin-stimulated adherent monocyte production of
TNF.alpha.. The A.sub.2A AR selective antagonist ZM241385 (100 nM)
antagonized the effects of JMR193 on TNF.alpha. production (FIG.
15).
Thus, DWH146e and JMR193 decrease LPS endotoxin-stimulated
TNF.alpha. production by human monocytes by a mechanism that is
dependent upon agonist binding to A.sub.2A adenosine receptors.
EXAMPLE 22
Activity of DWH-146e in Murine Peritonitis Model
Preliminary experiments with experimental peritonitis have involved
the injection of zymosan (Zym) as a potent stimulus of inflammation
(Y. Zhang et al., Eur. J. Pharmacol., 313, 237 (1996)). As shown in
FIG. 16, following injection of zymosan, the mean leukocyte
concentration as determined in a neubauer hemocytometer was
7,325.+-.1,893/mm.sup.3. Intraperitoneal injection of DWH-146e at a
dosage of 2.5 .mu.g/kg one hour prior to zymosan inhibited the
development of peritonitis with a mean.+-.SEM leukocyte
concentration of 2,012.+-.374/mm.sup.3 6 hours later (p<0.05).
Thus, these studies demonstrate that the A.sub.2A AR is
instrumental in mediating PMN traversal into the peritoneum
following zymosan challenge.
All publications, patents, and patent documents are incorporated by
reference herein, as though individually incorporated by reference.
The invention has been described with reference to various specific
and preferred embodiments and techniques. However, it should be
understood that many variations and modifications may be made while
remaining within the spirit and scope of the invention.
* * * * *