U.S. patent number 7,119,104 [Application Number 10/491,935] was granted by the patent office on 2006-10-10 for activator of peroxisome proliferator-activated receptor delta.
This patent grant is currently assigned to Nippon Chemiphar, Co., Ltd.. Invention is credited to Takashi Kanda, Seiichiro Masui, Shogo Sakuma, Tomio Yamakawa.
United States Patent |
7,119,104 |
Sakuma , et al. |
October 10, 2006 |
**Please see images for:
( Certificate of Correction ) ** |
Activator of peroxisome proliferator-activated receptor delta
Abstract
A compound represented by the formula (I) or a salt of the
compound, and a PPAR-.delta. activator which contains the compound
or salt as the active ingredient: ##STR00001## (wherein A
represents O or S; B.sup.1 represents N, etc.; B.sup.2 represents
O, etc.; each of X.sup.1 and X.sup.2 represents O, S, a bond, etc.;
Y represents C.sub.1-8 alkylene chain; Z represents O or S; R.sup.1
represents aryl, etc. which can have substituents; R.sup.2
represents C.sub.1-8 alkyl, etc.; R.sup.3 represents C.sub.1-8
alkyl, C.sub.2-8 alkenyl, etc.; each of R.sup.4 and R.sup.5
represents hydrogen, C.sub.1-8 alkyl, etc.; and R.sup.6 represents
hydrogen, etc.; provided that each of Z and R.sup.3 is attached to
the benzene ring, and X.sup.2 is not attached to the benzene
ring).
Inventors: |
Sakuma; Shogo (Saitama,
JP), Yamakawa; Tomio (Chiba, JP), Kanda;
Takashi (Chiba, JP), Masui; Seiichiro (Saitama,
JP) |
Assignee: |
Nippon Chemiphar, Co., Ltd.
(Tokyo, JP)
|
Family
ID: |
19133818 |
Appl.
No.: |
10/491,935 |
Filed: |
October 9, 2002 |
PCT
Filed: |
October 09, 2002 |
PCT No.: |
PCT/JP02/10472 |
371(c)(1),(2),(4) Date: |
October 29, 2004 |
PCT
Pub. No.: |
WO03/033493 |
PCT
Pub. Date: |
April 24, 2003 |
Prior Publication Data
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Document
Identifier |
Publication Date |
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US 20050096363 A1 |
May 5, 2005 |
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Foreign Application Priority Data
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Oct 12, 2001 [JP] |
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2001-315694 |
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Current U.S.
Class: |
514/311; 514/365;
546/167; 546/270.4; 514/397; 514/374; 514/373; 546/271.4;
546/272.1; 546/272.7; 548/181; 548/206; 548/236; 548/241;
548/311.4; 514/338 |
Current CPC
Class: |
C07D
413/14 (20130101); A61P 3/04 (20180101); A61P
3/10 (20180101); C07D 403/06 (20130101); A61P
3/06 (20180101); A61P 29/00 (20180101); C07D
409/06 (20130101); A61K 31/427 (20130101); A61P
19/10 (20180101); C07D 417/06 (20130101); A61K
31/423 (20130101); A61P 5/14 (20180101); C07D
413/12 (20130101); A61P 25/28 (20180101); A61P
9/10 (20180101); C07D 417/14 (20130101); C07D
413/06 (20130101); C07D 417/12 (20130101); A61P
9/00 (20180101); A61K 31/00 (20130101); A61P
43/00 (20180101); A61K 31/454 (20130101); C07D
405/06 (20130101); A61P 35/00 (20180101) |
Current International
Class: |
C07D
413/06 (20060101); A61K 31/4178 (20060101); A61K
31/423 (20060101); A61K 31/427 (20060101); C07D
413/14 (20060101); C07D 417/06 (20060101) |
Field of
Search: |
;546/167,270.4,271.4,272.1,272.7 ;548/181,206,236,241,311.4
;514/365,373,374,379,397 |
References Cited
[Referenced By]
U.S. Patent Documents
Foreign Patent Documents
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505322 |
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Sep 1992 |
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EP |
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WO 92/10468 |
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Jun 1992 |
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WO |
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WO 96/20935 |
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Jul 1996 |
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WO |
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WO 96/35688 |
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Nov 1996 |
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WO |
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WO 97/27190 |
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Jul 1997 |
|
WO |
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WO 97/28115 |
|
Aug 1997 |
|
WO |
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WO 01/79197 |
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Oct 2001 |
|
WO |
|
Other References
He, T.-C. et al., 1999, Cell 99:335-345. cited by other .
Isseman, I. et al., 1990, Nature 347:645-650. cited by other .
Kliewer, S. et al., 1992, Nature 358:771-774. cited by other .
Kliewer, S. et al., 1994, Proc Natl Acad Sci USA 91:7335-7359.
cited by other .
Lehmann, J. et al., 1997, J Biol Chem 272(6):3406-3410. cited by
other .
Mano H., et al., 2000, J Biol Chem 175:8126-8132. cited by
other.
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Primary Examiner: Powers; Fiona T.
Attorney, Agent or Firm: Day; Jones
Claims
The invention claimed is:
1. A compound having the following formula (I) or a salt thereof:
##STR00014## (wherein A is O, S or NR.sup.7 in which R.sup.7 is
hydrogen or C.sub.1-8 alkyl; B.sup.1 is CW or N in which W is
hydrogen or a bond; B.sup.2 is O, S or NR.sup.8 in which R.sup.8 is
hydrogen or C.sub.1-8 alkyl; each of X.sup.1 and X.sup.2 is O, S,
NH, NHC(.dbd.O), C(.dbd.O), C(.dbd.N--OR.sup.9), CH(OR.sup.10),
C.dbd.C, C.ident.C or a bond in which each of R.sup.9 and R.sup.10
is hydrogen or C.sub.1-8 alkyl; Y is a C.sub.1-8 alkylene chain,
which can be substituted with C.sub.1-8 alkyl or C.sub.1-8 alkyl
substituted with 1 3 halogens; Z is NH, O or S R.sup.1 is aryl,
which can be substituted with a group or atom selected from the
group consisting of C.sub.1-8 alkyl, C.sub.1-8 alkoxy, C.sub.1-8
alkyl substituted with 1 3 halogens, hydroxyl, nitro, amino,
phenyl, pyridyl and halogen, or a heterocyclic group having five to
eight membered ring comprising one to three hetero atoms selected
from the group consisting of nitrogen, oxygen and sulfur and the
other atoms consisting of carbon (benzene ring can be condensed
with the heterocyclic ring); R.sup.2 is C.sub.2-8 alkyl, C.sub.1-8
alkyl substituted with 1 3 halogens, C.sub.3-7 cycloalkyl,
C.sub.2-8 alkenyl, C.sub.2-8 alkynyl, alkyl (comprising C.sub.1-4
alkyl moiety) substituted with aryl, which can be substituted with
a group or atom selected from the group consisting of C.sub.1-8
alkyl, C.sub.1-8 alkoxy, C.sub.1-8 alkyl substituted with 1 3
halogens, hydroxyl, nitro, amino, phenyl, pyridyl and halogen, or
alkyl (comprising C.sub.1-4 alkyl moiety) substituted with a
heterocyclic group having five to eight membered ring (comprising
one to three hetero atoms selected from the group consisting of
nitrogen, oxygen and sulfur and the other atoms consisting of
carbon); R.sup.3 is halogen, trifluoromethyl, C.sub.1-8 alkyl,
C.sub.2-8 alkenyl or C.sub.2-8 alkynyl; each of R.sup.4 and R.sup.5
is hydrogen, C.sub.1-8 alkyl or C.sub.1-8 alkyl substituted with 1
3 halogens; and R.sup.6 is hydrogen, C.sub.1-8 alkyl substituted
with amino, C.sub.1-8 alkyl or alkali metal; provided that each of
Z and R.sup.3 is attached to the benzene ring, and X.sup.2 is not
attached to the benzene ring.
2. A compound or a salt of thereof defined in claim 1, wherein
R.sup.1 is attached to the 2nd position of the oxazole, thiazole or
imidazole ring.
3. A compound or a salt thereof defined in claim 1, wherein B.sup.1
is N, and B.sup.2 is O.
4. A compound or a salt thereof defined in claim 1, wherein R.sup.6
is hydrogen.
5. A compound or a salt thereof defined in claim 1, wherein X.sup.2
is a bond.
6. A compound or a salt thereof defined in claim 1, wherein X.sup.1
is a bond.
7. A compound or a salt thereof defined in claim 1, wherein R.sup.1
is aryl substituted with a group or atom selected from the group
consisting of C.sub.1-8 alkyl, C.sub.1-8 alkoxy, C.sub.1-8 alkyl
substituted with 1 3 halogens, hydroxyl, nitro, amino, phenyl,
pyridyl and halogen.
8. A compound or a salt thereof defined in claim 1, wherein R.sup.2
is C.sub.2-8 alkyl.
9. A compound or a salt thereof defined in claim 1, wherein R.sup.3
is C.sub.1-8 alkyl or C.sub.2-8 alkenyl.
10. A pharmaceutical composition which comprises a compound or a
salt thereof of claim 1 and a pharmaceutically acceptable carrier
or diluent.
Description
TECHNICAL FIELD
The present invention relates to an activator of peroxisome
proliferator activated receptor .delta..
PRIOR ART
The peroxisome is a small organ present in cells of animals and
plants, and its matrix contains various enzymes such as catalases.
Various compounds such as fibrates, herbicides, and phthalic acid
plasticizers are known as peroxisome proliferators which induce
proliferation of peroxisomes.
Isseman, et al. have identified a nuclear receptor which is
activated by peroxisome proliferator and called it peroxisome
proliferator activated receptor (PPAR).--Nature, 347, p645 650,
1990.
Three subtypes such as PPAR.alpha., PPAR.gamma. and PPAR.delta.
have been identified.--Proc. Natl. Acad. Sci. USA, 91, p7335 7359,
1994.
The above-mentioned fibrates used as the serum triglyceride (TG)
lowering drug can modulates PPAR.delta. activity.
Further, thiazolidine compounds (Troglitazone, Rosiglitazone,
Pioglitazone) useful in the treatment of diabetes are also known as
ligands of PPAR.gamma..
It is reported that several compounds such as GW-2433 (Glaxo
Wellcome), L-165041 (Merck), and YM-16638 (Yamanouchi
Pharmaceutical) activate PPAR.delta.. Each formula is as
follows:
##STR00002##
WO 92/10468 describes that GW-2433 can be used for prevention and
treatment of atherosclerosis.
WO 97/28115 describes that L-165041 can be used for treatment of
diabetes and suppression of obesity.
WO 99/04815 describes that YM-16638 shows effects for reducing
serum cholesterol and reducing LDL cholesterol.
Recently, JBC, 272(6), p3406 3410, 1997 and Cell, 99, p335 345,
1999 describe proposal for application of PPAR .delta. ligand as an
anti-cancer agent and an anti-inflammatory agent.
The following compounds A to E have a structure similar to the
compound of the present invention (mentioned below), in more detail
a benzisoxazole derivative of the general formula (I). The compound
A is disclosed in Japanese Patent No. 2,581,523, the compound B is
disclosed in WO 98/28254, the compound C is disclosed in Japanese
Patent Provisional Publication No. 8(1996)-311065, the compound D
is disclosed in WO 97/27190, and the compound E is disclosed in WO
96/20935. Each formula is as follows:
##STR00003##
In the compounds A, B and C, carboxyl or cyano is linked by an
alkylene chain (which can be substituted with ethoxy or propylthio
at the .alpha.-position) to the right side of the benzisoxazole or
benzofuran ring.
The compound of the invention has a structure different from the
compounds A, B and C. In the compound of the invention, acetic acid
or a 2-alkylpropionic acid is linked by an ether or thioether bond
to the 5th to 7th position of the benzisoxazole ring.
It has been reported that the compounds A, B and C have an effect
of improving insulin resistance or decreasing blood glucose.
However, no mention is given with respect to an effect as
PPAR.delta. ligand.
In the compound D, benzofuran ring is linked by an alkylene chain
to the 1st position of the indole ring.
The compound of the invention has a structure different from the
compound D. In the compound of the invention, the thiazole or
oxazole ring is linked by an alkylene chain to the 3rd position of
the benzisoxazole ring. WO 97/27190 describes that the ACAT (acyl
coenzyme A--cholesterol acyltransferase). However, no mention is
given with respect to an effect as PPAR.delta. ligand.
In the compound E, the oxazole ring is substituted with only
phenethyl at 4th position, and the benzene ring moiety of the
benzofuran ring has only methoxycarbonyl-methoxy. The compound of
the invention has a structure different from the compound E. In the
compound of the invention, the oxazole (or thiazole) ring has two
substituent groups such as a substituted phenyl and isopropyl, and
the benzene ring moiety of the benzofuran ring (benzisoxazole ring)
has a substituent group such as propyl, propenyl in addition to
alkoxy substituted with carboxyl. WO 96/20935 describes that the
compound E has an antagonistic effect against TXA.sub.2 receptor.
However, no mention is given with respect to an effect as
PPAR.delta. ligand.
The present inventors have been filed WO 01/79197, which relates to
benzisoxazole derivatives. In the compounds of the Examples in the
patent application, the benzisoxazole ring has no substituent. On
the other hand, the compound of the present invention has
benzisoxazole ring having at least one substituent.
An object of the present invention is to provide a compound having
the following general formula (I), which has an effect of
activating peroxisome proliferator activated receptor .delta..
[Disclosure of Invention]
The invention resides in a compound having the following general
formula (I) or a salt thereof:
##STR00004##
(wherein A is O, S or NR.sup.7 in which R.sup.7 is hydrogen or
C.sub.1-8 alkyl;
B.sup.1 is CW or N in which W is hydrogen or a bond;
B.sup.2 is O, S or NR.sup.8 in which R.sup.8 is hydrogen or
C.sub.1-8 alkyl;
each of X.sup.1 and X.sup.2 is O, S, NH, NHC(.dbd.O)., C(.dbd.O),
C(.dbd.N--OR.sup.9, CH(OR.sup.10), C.dbd.C, C.ident.C or a bond in
which each of R.sup.9 and R.sup.10 is hydrogen or C.sub.1-8
alkyl;
Y is a C.sub.1-8 alkylene chain, which can be substituted with
C.sub.1-8 alkyl or C.sub.1-8 alkyl substituted with 1 3
halogens;
Z is NH, O or S
R.sup.1 is aryl, which can be substituted with a group or atom
selected from the group consisting of C.sub.1-8 alkyl, C.sub.1-8
alkoxy, C.sub.1-8 alkyl substituted with 1 3 halogens, hydroxyl,
nitro, amino, phenyl, pyridyl and halogen, or a heterocyclic group
having five to eight membered ring comprising one to three hetero
atoms selected from the group consisting of nitrogen, oxygen and
sulfur and the other atoms consisting of carbon (benzene ring can
be condensed with the heterocyclic ring);
R.sup.2 is C.sub.2-8 alkyl, C.sub.1-8 alkyl substituted with 1 3
halogens, C.sub.3-7 cycloalkyl, C.sub.2-8 alkenyl, C.sub.2-8
alkynyl, alkyl (comprising C.sub.1-4 alkyl moiety) substituted with
aryl, which can be substituted with a group or atom selected from
the group consisting of C.sub.1-8 alkyl, C.sub.1-8 alkoxy,
C.sub.1-8 alkyl substituted with 1 3 halogens, hydroxyl, nitro,
amino, phenyl, pyridyl and halogen, or alkyl (comprising C.sub.1-4
alkyl moiety) substituted with a heterocyclic group having five to
eight membered ring (comprising one to three hetero atoms selected
from the group consisting of nitrogen, oxygen and sulfur and the
other atoms consisting of carbon);
R.sup.3 is halogen, trifluoromethyl, C.sub.1-8 alkyl, C.sub.2-8
alkenyl or C.sub.2-8 alkynyl;
each of R.sup.4 and R.sup.5 is hydrogen, C.sub.1-8 alkyl or
C.sub.1-8 alkyl substituted with 1 3 halogens; and
R.sup.6 is hydrogen, C.sub.1-8 alkyl substituted with amino,
C.sub.1-8 alkyl or alkali metal;
provided that each of Z and R.sup.3 is attached to the benzene
ring, and X.sup.2 is not attached to the benzene ring.
The invention also provides an activator of peroxisome proliferator
activated receptor .delta., which contains as an effective
component a compound of the formula (I) or a salt thereof.
DETAILED DESCRIPTION OF THE INVENTION
In the formula (I), R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7,
R.sup.8, R.sup.9, R.sup.10, the substituent of the alkylene chain
of Y, the substituent of the aryl and the heterocyclic group of
R.sup.3, the substituent of the alkyl group substituted with aryl
of R.sup.2, and the substituent of the alkyl group substituted with
a heterocyclic group of R.sup.2 can be an alkyl group having 1 8
carbon atoms. Examples of the alkyl groups include methyl, ethyl,
propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl and hexyl.
R.sup.2 can be an alkyl group having 2 8 carbon atoms. Examples of
the alkyl groups include ethyl, propyl, iso-propyl, butyl,
isobutyl, t-butyl, pentyl and hexyl.
R.sup.2, R.sup.4, R.sup.5, the substituent of the alkylene chain of
Y, the substituent of the aryl or heterocyclic group of R.sup.1,
the substituent of the alkyl group substituted with aryl of
R.sup.2, and the substituent of the alkyl group substituted with a
heterocyclic group of R.sup.2 can be an alkyl groups having 1 8
carbon atoms substituted with 1 3 halogens. Examples of the
haloalkyl groups include methyl, ethyl, propyl, isopropyl, butyl,
and t-butyl which are substituted with 1 3 halogens such as
fluorine, chlorine, and bromine. Trifluoromethyl, chloromethyl,
2-chloroethyl, 2-bromoethyl and 2-fluoroethyl are preferred.
R.sup.2 and R.sup.3 can be an alkenyl group having 2 8 carbon
atoms. Examples of the alkenyl groups include vinyl and allyl.
R.sup.2 and R.sup.3 can be an alkynyl group having 2 8 carbon
atoms. Examples of the alkynyl groups include propargyl.
R.sup.3 can be a halogen atom. Examples of the halogen atoms
include fluorine, chlorine and bromine.
R.sup.2 can be a cycloalkyl group having 3 7 carbon atoms. Examples
of the cycloalkyl groups include cyclopropyl, cyclopentyl and
cyclohexyl.
The substituent of the aryl or heterocyclic group of R.sup.1, the
substituent of the alkyl group substituted with aryl of R.sup.2,
and the substituent of the alkyl group substituted with a
heterocyclic group of R.sup.2 can be an alkoxy groups having 1 8
carbon atoms. Examples of the alkoxy groups include methoxy,
ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, t-butoxy, pentyloxy
and hexyloxy.
R.sup.1 and the aryl moiety of the aryl substituted with alkyl of
R.sup.2 can be an aryl group. Examples of the aryl groups include
phenyl and naphthyl.
R.sup.1 and the substituent of the alkyl group of R.sup.2 can be a
heterocyclic group having five to eight membered ring. Examples of
the heterocyclic groups include pyridyl, thienyl, furyl, thiazolyl
and quinolyl.
R.sup.1 can be a heterocyclic group having five to eight membered
ring comprising one to three hetero atoms selected from the group
consisting of nitrogen, oxygen and sulfur and the other atoms
consisting of carbon. A benzene ring can be condensed with the
heterocyclic ring. Examples of the condensed rings include
quinoline ring and benzothiophene ring.
Y can be an alkylene chain having 1 to 8 carbon atoms. Examples of
the alkylene chains include methylene and ethylene.
R.sup.3 can be one to three groups. Two or three groups of R.sup.3
can be different from each other.
R.sup.6 can be an alkyl group having 1 8 carbon atoms substituted
with amino. Examples of the aminoalkyl groups include methyl,
ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl and
hexyl which are substituted with an amino group such as piperidino,
pyrrolidino, dimethylamino, and diethylamino.
(1) A preferred compound of the invention is a compound of the
formula (I) or salt thereof, in which R.sup.1 is attached to the
2nd position of the oxazole, thiazole or imidazole ring.
(2) Another preferred compound of the invention is a compound of
the formula (I), a salt thereof or (1), in which B.sup.1 is N, and
B.sup.2 is O.
(3) A further preferred compound of the invention is a compound of
the formula (I), a salt thereof, (1) or (2), in which R.sup.6 is
hydrogen.
(4) A furthermore preferred compound of the invention is a compound
of the formula (I), a salt thereof, (1), (2) or (3), in which
X.sup.2 is a bond.
(5) A still further preferred compound of the invention is a
compound of the formula (I), a salt thereof, (1), (2), (3) or (4),
in which X.sup.1 is a bond.
(6) A still further preferred compound of the invention is a
compound of the formula (I), a salt thereof, (1), (2), (3), (4) or
(5), in which R.sup.1 is aryl substituted with a group or atom
selected from the group consisting of C.sub.1-8 alkyl, C.sub.1-8
alkoxy, C.sub.1-8 alkyl substituted with 1 3 halogens, hydroxyl,
nitro, amino, phenyl, pyridyl and halogen.
(7) A still further preferred compound of the invention is a
compound of the formula (I), a salt thereof, (1), (2), (3), (4),
(5) or (6), in which R.sup.2 is C.sub.2-8 alkyl.
(8) A still further preferred compound of the invention is a
compound of the formula (I), a salt thereof, (1), (2), (3), (4),
(5), (6) or (7), in which R.sup.3 is C.sub.1-8 alkyl or C.sub.2-8
alkenyl.
The compound of the formula (I) can be in the form of
pharmaceutically acceptable salts such as alkali metal salts, e.g.,
sodium salt and potassium salt.
The processes for preparing the benzisoxazole derivative of the
formula (I) are described below.
[Synthetic Process 1]
##STR00005##
(In the formulas, R is an alkyl group having 1 6 carbon atoms such
as methyl, ethyl, Q is a releasing group such as chlorine, bromine,
and R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, A, X.sup.1, Y,
X.sup.2 and Z are those described hereinbefore).
The hydroxyl (or mercapto)benzisoxazole derivative of the formula
(III) can be prepared by diazotizing the aminobenzisoxazole
derivative of the formula (II) with sodium nitrite and a mineral
acid (sulfuric acid) while cooling with ice, and decomposing the
product with sulfuric acid where Z is oxygen, or reacting the
product with potassium ethylxanthate and heating it where Z is
sulfur.
The benzisoxazole derivative of the formula (V) according to the
invention can be prepared by reacting the compound of the formula
(III) with the acetic ester derivative of the formula (IV) in the
presence of a base such as potassium carbonate.
The benzisoxazole derivative of the formula (VI) according to the
invention can be prepared by subjecting the benzisoxazole
derivative of the formula (V) according to the invention to
hydrolysis in the presence of lithium hydroxide or potassium
hydroxide.
In the case that R.sup.3 is allyl, the starting material
represented by the formula (III) can be synthesized according to
the following reaction scheme.
##STR00006##
(In the formulas, A, R.sup.1, R.sup.2, X.sup.1, X.sup.2 and Y are
those described hereinbefore).
[Synthetic Process 2]
The benzisoxazole derivative of the invention can be prepared
according to the following reaction scheme.
##STR00007##
(In the formulas, A, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5,
Z, Q and R are those described hereinbefore).
[Synthetic Process 3]
The benzisoxazole derivative of the invention can also be prepared
according to the following reaction scheme.
##STR00008##
(In the formulas, R, Q, R.sup.1, R.sup.2, R.sup.3, R.sup.4,
R.sup.5, B.sup.2, A and Z are those described hereinbefore).
The staring materials of the above-mentioned reaction scheme,
namely hydroxyindolealdehyde and hydroxybenzothiophenealdehyde can
be prepared, for example by referring the processes disclosed in WO
96/35688, EP 505322.
[Synthetic Process 4]
The compound of the invention wherein R.sup.3 is propyl can be
prepared according to the following reaction scheme.
##STR00009##
(In the formulas, A, R.sup.1, R.sup.2, R.sup.4, R.sup.5, X.sup.1,
X.sup.2, Y, Z and R are those described hereinbefore).
The other compounds represented by the formula (I) can also be
prepare according to an analogous method.
The prepared compounds according to the invention are set forth in
Tables 1 to 24.
(Typical Compounds 1)
The compounds of the invention wherein X.sup.2 is a bond, B.sup.1
is N, B.sup.2 is O, R.sup.6 is H are shown in Tables 1 to 5 (in
which R.sup.1 is a substituent at the 2nd position of the
imidazole, oxazole or thiazole ring, and R.sup.2 is a substituent
at the 4th position of the imidazole, oxazole or thiazole
ring).
##STR00010##
TABLE-US-00001 TABLE 1 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (4-CF.sub.3) Isopropyl Allyl (7) Me Me S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-Cl) Isopropyl Methyl (7) H H S
Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-t-Bu) Isopropyl Methyl (5,
7) Me Me O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,4-Cl) Isopropyl
Propyl (7) H H S Bond CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl
(3,4-Me) Isopropyl Butyl (7) Me Et S Bond CH.sub.2--CH.sub.2 S (6)
Phenyl (2,4-F) Isopropyl Hexyl (7) Me H S Bond CH.sub.2--CH.sub.2 O
(6) Phenyl (2,3-F) Isopropyl Isopropyl (7) Me Et O NHCO
CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Isopropyl 2-Propynyl (7)) H
H NH Bond CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isopropyl
2-Butenyl (7) H H NMe Bond CH.sub.2--CH.sub.2 O (6) phenyl
TABLE-US-00002 TABLE 2 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) 4-Cyclo- Isopropyl 2-Pentenyl (7) Me Me S Bond
CH.sub.2--CH.sub.2 S (6) hexylphenyl (4-NO.sub.2) Isopropyl
Isobutyl (5) H H S Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(4-NH.sub.2) Isopropyl t-Butyl (5) Me Me O Bond CH.sub.2--CH.sub.2
O (6) Phenyl (4-NMe.sub.2) Isopropyl Allyl (5) H H S CONH
CH.sub.2--CH.sub.2 O (6) Phenyl (3,4,5-Me) Isopropyl Methyl (5) Me
Me S Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,5-Me,4-OH) Isopropyl
Methyl (5, 7) Me H S Bond CH.sub.2--CH.sub.2 S (6) Phenyl 3-Pyridyl
Isopropyl Propyl (7) Me Et O Bond CH.sub.2--CH.sub.2 O (6)
2-Pyridyl Isopropyl Butyl (7) H H NH CH(OH) CH.sub.2--CH.sub.2 O
(6) 3-Pyridyl Isopropyl Hexyl (7) H H NMe Bond CH.sub.2--CH.sub.2 O
(6)
TABLE-US-00003 TABLE 3 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) 2-Naphthyl Isopropyl Isopropyl (7) Me Me S Bond
CH.sub.2--CH.sub.2 O (6) 1-Naphthyl Isopropyl 2-Propynyl (7) H H S
Bond CH.sub.2--CH.sub.2 S (6) 2-Quinolyl Isopropyl 2-Butenyl (7) Me
Me O CO CH.sub.2--CH.sub.2 O (6) (4-CF.sub.3) Propyl 2-Pentenyl (7)
H H S Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-Cl) Hexyl Isobutyl
(5) Me Me S Bond CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl (4-t-Bu)
Butyl t-Butyl (5) Me H S Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(3,4-Cl) Isobutyl Allyl (7) Me Et O Bond CH.sub.2--CH.sub.2 S (6)
Phenyl (3,4-Me) Ethyl Methyl (7) H H NH Bond CH.sub.2--CH.sub.2 O
(6) Phenyl (2,4-F) Propyl Methyl (5, 7) H H NMe Bond
CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl
TABLE-US-00004 TABLE 4 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (2,3-F) Hexyl Propyl (7) Me Me S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Butyl Butyl (7) H H S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isobutyl Hexyl (7) Me Me
O Bond CH.sub.2--CH.sub.2 S (6) phenyl 4-Cyclo- Ethyl Isopropyl (7)
H H S Bond CH.sub.2--CH.sub.2 O (6) hexylphenyl (4-NO.sub.2) Propyl
2-Propynyl (7) Me Me S Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(4-NH.sub.2) Hexyl 2-Butenyl (7) Me H S NMe CH.sub.2--CH.sub.2 O
(6) Phenyl (4-NMe.sub.2) Butyl 2-Pentenyl (7) Me Et O Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (3,4,5-Me) Isobutyl Isobutyl (5) H
H NH Bond CH.sub.2--CH.sub.2 S (6) Phenyl (3,5-Me,4-OH) Ethyl
t-Butyl (5) H H NMe Bond CH.sub.2--CH.sub.2--CH.sub.2 O (6)
Phenyl
TABLE-US-00005 TABLE 5 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (3,4-Cl) Isopropyl Propyl (4) H H S Bond
CH.sub.2--CH.sub.2 O (5) Phenyl (3,4-Me) Trifluoro- Butyl (4) Me Me
S NH--CH.sub.2 CH.sub.2--CH.sub.2 S (5) Phenyl methyl (2,4-F)
Isopropyl Hexyl (4) Me H S Bond CH.sub.2--CH.sub.2 O (5) Phenyl
(2,3-F) Isopropyl Isopropyl (4) Me Et O Bond CH.sub.2--CH.sub.2 O
(5) Phenyl (4-OMe) Benzyl 2-Propynyl (4) H H NH Bond
CH.sub.2--CH.sub.2 O (5) Phenyl 4-Acetyl- Isopropyl 2-Butenyl (4) H
H NMe Bond CH.sub.2--CH.sub.2 O (5) phenyl 4-Cyclo- Isopropyl
2-Pentenyl (4) Me Me S Bond CH.sub.2--CH.sub.2 S (5)
hexylphenyl
(Typical Compounds 2)
The compounds of the invention wherein X.sup.2 is a bond, B.sup.1
is N, B.sup.2 is O, R.sup.6 is H are shown in Tables 6 to 10 (in
which R.sup.1 is a substituent at the 2nd position of the
imidazole, oxazole or thiazole ring, and R.sup.2 is a substituent
at the 5th position of the imidazole, oxazole or thiazole
ring).
##STR00011##
TABLE-US-00006 TABLE 6 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (2,4-Cl) Isopropyl Allyl (7) H H O Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-Cl) Isopropyl Allyl (7) Me Me
O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-Cl) Isopropyl Propyl
(7) H H O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (2-OH,4-Cl)
Isopropyl Propyl (7) H H S CH(OH) CH.sub.2--CH.sub.2 O (6) Phenyl
(3,4-Me) Isopropyl Methyl (5, 7) Me Me S Bond CH.sub.2--CH.sub.2 S
(6) Phenyl (2,4-Me) Isopropyl Hexyl (7) Me H S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (2,3-F) Isopropyl Isopropyl (7) Me
Et O CO CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Isopropyl
2-Propynyl (7)) H H NH Bond CH.sub.2--CH.sub.2 O (6) Phenyl
4-Acetyl- Isopropyl 2-Butenyl (7) H H NMe Bond CH.sub.2--CH.sub.2 O
(6) phenyl
TABLE-US-00007 TABLE 7 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) 4-Cyclohexylphenyl Isopropyl 2-Pentenyl (7) Me Me S
NHCO CH.sub.2--CH.sub.2 S (6) (4-NO.sub.2)Phenyl Isopropyl Isobutyl
(5) H H S CONH CH.sub.2--CH.sub.2 O (6) (4-NH.sub.2)Phenyl
Isopropyl t-Butyl (5) Me Me O Bond CH.sub.2--CH.sub.2 O (6)
(4-NMe.sub.2)Phenyl Isopropyl Allyl (7) H H S Bond
CH.sub.2--CH.sub.2 O (6) (3,4,5-Me)Phenyl Isopropyl Methyl (7) Me
Me S CO CH.sub.2--CH.sub.2--CH.sub.2 O (6) (3,5-Me,4-OH)Phenyl
Isopropyl Methyl (5, 7) Me H S Bond CH.sub.2--CH.sub.2 S (6)
3-Pyridyl Isopropyl Propyl (7) Me Et O Bond CH.sub.2--CH.sub.2 O
(6) 2-Pyridyl Isopropyl Butyl (7) H H NH Bond CH.sub.2--CH.sub.2 O
(6) 3-Pyridyl Isopropyl Hexyl (7) H H NMe Bond CH.sub.2--CH.sub.2 O
(6)
TABLE-US-00008 TABLE 8 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) 2-Naphthyl Isopropyl Isopropyl (7) Me Me S Bond
CH.sub.2--CH.sub.2 O (6) 1-Naphthyl Isopropyl 2-Propynyl (7) H H S
Bond CH.sub.2--CH.sub.2 S (6) 2-Quinolyl Isopropyl 2-Butenyl (7) Me
Me O Bond CH.sub.2--CH.sub.2 O (6) (4-CF.sub.3) Propyl 2-Pentenyl
(7) H H S NH CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl (4-Cl) Hexyl
Isobutyl (5) Me Me S Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-t-Bu)
Butyl t-Butyl (5) Me H S Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(3,4-Cl) Isobutyl Methyl (5, 7) Me Et O Bond CH.sub.2--CH.sub.2 S
(6) Phenyl (3,4-Me) Ethyl Methyl (7) H H NH Bond CH.sub.2--CH.sub.2
O (6) Phenyl (2,4-F) Propyl Methyl (5, 7) H H NMe Bond
CH.sub.2--CH.sub.2 O (6) Phenyl
TABLE-US-00009 TABLE 9 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (2,3-F) Hexyl Propyl (7) Me Me S CH.dbd.
CH.sub.2--CH.sub.2 O (6) Phenyl CH (4-OMe) Butyl Butyl (7) H H S
Bond CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isobutyl Hexyl (7)
Me Me O NMe CH.sub.2--CH.sub.2-- CH.sub.2 S (6) phenyl 4-Cyclo-
Ethyl Isopropyl (7) H H S Bond CH.sub.2--CH.sub.2 O (6) hexylphenyl
(4-NO.sub.2) Propyl 2-Propynyl (7) Me Me S Bond CH.sub.2--CH.sub.2
O (6) Phenyl (4-NH.sub.2) Hexyl 2-Butenyl (7) Me H S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-NMe.sub.2) Butyl 2-Pentenyl (7)
Me Et O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,4,5-Me) Isobutyl
Isobutyl (5) H H NH CH(OH) CH.sub.2--CH.sub.2--CH.sub.2 S (6)
Phenyl (3,5-Me,4-OH) Ethyl t-Butyl (5) H H NMe Bond
CH.sub.2--CH.sub.2 O (6) Phenyl
TABLE-US-00010 TABLE 10 R.sup.3 (position Z (position R.sup.1
R.sup.2 of substitution) R.sup.4 R.sup.5 A X.sup.1 Y of
substitution) (2,4-Cl) Isopropyl Allyl (4) H H O Bond
CH.sub.2--CH.sub.2 O (5) Phenyl (2,4-Cl) Trifluoro- Allyl (4) Me Me
O Bond CH.sub.2--CH.sub.2 O (5) Phenyl methyl (2,4-Cl) Isopropyl
Propyl (4) H H O Bond CH.sub.2--CH.sub.2 O (5) Phenyl (2-OH,4-Cl)
Isopropyl Propyl (4) H H S Bond CH.sub.2--CH.sub.2 O (5) Phenyl
(3,4-Me) Benzyl Methyl (4) Me Me S Bond CH.sub.2--CH.sub.2 S (5)
Phenyl (2,4-Me) Isopropyl Hexyl (4) Me H S Bond CH.sub.2--CH.sub.2
O (5) Phenyl (2,3-F) Isopropyl Isopropyl (4) Me Et O Bond
CH.sub.2--CH.sub.2 O (5) Phenyl
(Typical Compounds 3)
The compounds of the invention wherein X.sup.2 is a bond, R.sup.6
is H are shown in Tables 11 to 15 (in which R.sup.1 is a
substituent at the 2nd position of the imidazole, oxazole or
thiazole ring, and R.sup.2 is a substituent at the 4th position of
the imidazole, oxazole or thiazole ring).
##STR00012##
In Tables 11 to 15, (*) means the position of substitution.
TABLE-US-00011 TABLE 11 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (2,4-Cl) Isopropyl Allyl (7) H H
O CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-Cl) Isopropyl
Allyl (7) Me Me O CH O CO CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-Cl)
Isopropyl Propyl (7) H H O CH S Bond CH.sub.2--CH.sub.2 O (6)
Phenyl (2-OH,4-Cl) Isopropyl Propyl (7) H H S CH NH Bond
CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl (3,4-Me) Isopropyl Me (5,
7) Me Me S N S Bond CH.sub.2--CH.sub.2 S (6) Phenyl (2,4-Me)
Isopropyl Hexyl (7) Me H S CH NMe CONH CH.sub.2--CH.sub.2 O (6)
Phenyl (2,3-F) Isopropyl Isopropyl (7) Me Et O CH NPr Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Isopropyl 2-Propynyl (7) H
H NH CH O CH(OH) CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl-
Isopropyl 2-Butenyl (7) H H NMe CH O Bond CH.dbd.CH O (6)
phenyl
TABLE-US-00012 TABLE 12 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) 4-Cyclohexyl- Isopropyl
2-Pentenyl (7) Me Me S CH S Bond CH.sub.2--CH.sub.2 S (6) phenyl
(4-NO.sub.2) Isopropyl Isobutyl (5) H H S CH NH Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-NH.sub.2) Isopropyl t-Butyl (5)
Me Me O N S NHCO CH.sub.2--CH.sub.2 O (6) Phenyl (4-NMe.sub.2)
Isopropyl Allyl (7) H H S CH NMe Bond CH.sub.2--CH.sub.2 O (6)
Phenyl (3,4,5-Me) Isopropyl Me (7) Me Me S CH NPr Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (3,5-Me,4-OH) Isopropyl Me (5,7) Me
H S CH O CH(OH) CH.sub.2--CH.sub.2--CH.sub.2 S (6) Phenyl 3-Pyridyl
Isopropyl Propyl (7) Me Et O CH O Bond CH.sub.2--CH.sub.2 O (6)
2-Pyridyl Isopropyl Butyl (7) H H NH CH S Bond
CH.sub.2--CH.sub.2--CH.sub.2 O (6) 3-Pyridyl Isopropyl Hexyl (7) H
H NMe CH NH Bond CH.sub.2--CH.sub.2 O (6)
TABLE-US-00013 TABLE 13 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) 2-Naphthyl Isopropyl Isopropyl
(7) Me Me S N S CONH CH.sub.2--CH.sub.2 O (6) 1-Naphthyl Isopropyl
2-Propynyl (7) H H S CH NMe Bond CH.sub.2--CH.sub.2 S (6)
2-Quinolyl Isopropyl 2-Butenyl (7) Me Me O CH NPr Bond
CH.sub.2--CH.sub.2 O (6) (4-CF.sub.3)Phenyl Propyl 2-Pentenyl (7) H
H S CH O Bond CH.sub.2--CH.sub.2 O (6) (4-Cl)Phenyl Hexyl Isobutyl
(7) Me Me S CH O Bond CH.sub.2--CH.sub.2 O (6) (4-t-Bu) Butyl
t-Butyl (7) Me H S CH S Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(3,4-Cl) Isobutyl Me (5, 7) Me Et O CH NH Bond CH.sub.2--CH.sub.2 S
(6) Phenyl (3,4-Me) Et Me (7) H H NH N S Bond CH.dbd.CH O (6)
Phenyl (2,4-F) Propyl Me (6, 7) H H NMe CH NMe Bond
CH.sub.2--CH.sub.2 O (6) Phenyl
TABLE-US-00014 TABLE 14 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (2,3-F) Hexyl Propyl (7) Me Me S
CH NPr Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Butyl Butyl (7)
H H S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isobutyl
Hexyl (7) Me Me O CH O Bond CH.sub.2--CH.sub.2 S (6) phenyl
4-Cyclo- Et Isopropyl (7) H H S CH S CH(OH) CH.sub.2--CH.sub.2 O
(6) hexylphenyl (4-NO.sub.2) Propyl 2-Propynyl (7) Me Me S CH NH
Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-NH.sub.2) Hexyl 2-Butenyl
(7) Me H S N S Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-NMe.sub.2)
Butyl 2-Pentenyl (7) Me Et O CH NMe Bond CH.sub.2--CH.sub.2 O (6)
Phenyl (3,4,5-Me) Isobutyl Isobutyl (7) H H NH CH NPr Bond
CH.sub.2--CH.sub.2 S (6) Phenyl
TABLE-US-00015 TABLE 15 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (3,5-Me,4-OH) Et t-Butyl (5) H H
NMe CH O NH CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-Cl)
Isopropyl Allyl (5) H H O CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(2,4-Cl) Trifluoro- Allyl (5) Me Me O CH S NMe
CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl ethyl (2,4-Cl) Benzyl
Propyl (5) H H O CH NH Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(2-OH,4-Cl) Isopropyl Propyl (5) H H S N S Bond CH.sub.2--CH.sub.2
O (6) Phenyl (3,4-Me) Isopropyl Me (5) Me Me S CH NMe Bond
CH.sub.2--CH.sub.2 S (6) Phenyl (2,4-Me) Isopropyl Hexyl (5) Me H S
CH NPr Bond CH.sub.2--CH.sub.2 O (6) Phenyl (2,3-F) Isopropyl
Isopropyl (5) Me Et O CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl
(Typical Compounds 4)
The compounds of the invention wherein X.sup.2 is a bond, R.sup.6
is H are shown in Tables 16 to 20 (in which R.sup.1 is a
substituent at the 2nd position of the imidazole, oxazole or
thiazole ring, and R.sup.2 is a substituent at the 5th position of
the imidazole, oxazole or thiazole ring).
##STR00013##
In Tables 16 to 20, (*) means the position of substitution.
TABLE-US-00016 TABLE 16 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (4-CF.sub.3) Isopropyl Allyl (7)
Me Me S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-Cl) Isopropyl
Me (7) H H S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-t-Bu)
Isopropyl Me (5, 7) Me Me O CH S CH(OH) CH.sub.2--CH.sub.2 O (6)
Phenyl (3,4-Cl) Isopropyl Propyl (7) H H S CH NH Bond
CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl (3,4-Me) Isopropyl Butyl
(7) Me Et S N S Bond CH.dbd.CH S (6) Phenyl (2,4-F) Isopropyl Hexyl
(7) Me H S CH NMe Bond CH.sub.2--CH.sub.2 O (6) Phenyl (2,3-F)
Isopropyl Isopropyl (7) Me Et O CH NPr CO CH.sub.2--CH.sub.2 O (6)
Phenyl (4-OMe) Isopropyl 2-Propynyl (7) H H NH CH O Bond
CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isopropyl 2-Butenyl (7) H
H NMe CH O Bond CH.sub.2--CH.sub.2 O (6) phenyl
TABLE-US-00017 TABLE 17 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) 4-Cyclo- Isopropyl 2-Pentenyl (7)
Me Me S CH S Bond CH.sub.2--CH.sub.2 S (6) hexylphenyl (4-NO.sub.2)
Isopropyl Isobutyl (5) H H S CH NH CONH CH.sub.2--CH.sub.2 O (6)
Phenyl (4-NH.sub.2) Isopropyl t-Butyl (5) Me Me O N S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-NMe.sub.2) Isopropyl Allyl (7) H
H S CH NMe Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,4,5-Me)
Isopropyl Me (7) Me Me S CH NPr Bond CH.sub.2--CH.sub.2 O (6)
Phenyl (3,5-Me,4-OH) Isopropyl Me (5, 7) Me H S CH O CO
CH.sub.2--CH.sub.2--CH.sub.2 S (6) Phenyl 3-Pyridyl Isopropyl
Propyl (7) Me Et O CH O Bond CH.sub.2--CH.sub.2 O (6) 2-Pyridyl
Isopropyl Butyl (7) H H NH CH S CO CH.dbd.CH--CH.sub.2--CH.sub.2 O
(6) 3-Pyri dyl Isopropyl Hexyl (7) H H NMe CH NH Bond
CH.sub.2--CH.sub.2 O (6)
TABLE-US-00018 TABLE 18 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) 2-Naphthyl Isopropyl Isopropyl
(7) Me Me S N S Bond CH.sub.2--CH.sub.2 O (6) 1-Naphthyl Isopropyl
2-Propynyl (7) H H S CH NMe NMe CH.sub.2--CH.sub.2--CH.sub.2 S (6)
2-Quinolyl Isopropyl 2-Butenyl (7) Me Me O CH NPr Bond
CH.sub.2--CH.sub.2 O (6) (4-CF.sub.3) propyl 2-Pentenyl (7) H H S
CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-Cl) Hexyl Isobutyl (7)
Me Me S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-t-Bu) Butyl
t-Butyl (7) Me H S CH S NMe CH.sub.2--CH.sub.2--CH.sub.2 O (6)
Phenyl (3,4-Cl) Isobutyl Allyl (7) Me Et O CH NH Bond
CH.sub.2--CH.sub.2 S (6) Phenyl (3,4-Me) Et Me (7) H H NH N S Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (2,4-F) Propyl Me (6, 7) H H NMe CH
NMe Bond CH.sub.2--CH.sub.2 O (6) Phenyl
TABLE-US-00019 TABLE 19 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (2,3-F) Hexyl Propyl (7) Me Me S
CH NPr Bond CH.sub.2--CH.sub.2 O (6) Phenyl (4-OMe) Butyl Butyl (7)
H H S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl- Isobutyl
Hexyl (7) Me Me O CH O Bond CH.sub.2--CH.sub.2 S (6) phenyl
4-Cyclo- Et Isopropyl (7) H H S CH S Bond CH.sub.2--CH.sub.2 O (6)
hexylphenyl (4-NO.sub.2) Propyl 2-Propynyl (7) Me Me S CH NH Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (4-NH.sub.2) Hexyl 2-Butenyl (7) Me
H S N S CH(OH) CH.sub.2--CH.sub.2--CH.sub.2 O (6) Phenyl
(4-NMe.sub.2) Butyl 2-Pentenyl (7) Me Et O CH NMe Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (3,4,5-Me) Isobutyl Isobutyl (5) H
H NH CH NPr Bond CH.sub.2--CH.sub.2 S (6) Phenyl
TABLE-US-00020 TABLE 20 R.sup.1 R.sup.2 R.sup.3 (*) R.sup.4 R.sup.5
A B.sup.1 B.sup.2 X.sup.1 Y Z (*) (3,5-Me,4-OH) Et t-Butyl (5) H H
NMe CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,4-Cl) Isopropyl
Propyl (5) H H S CH O Bond CH.sub.2--CH.sub.2 O (6) Phenyl (3,4-Me)
Trifluoro- Butyl (5) Me Me S CH S Bond CH.sub.2--CH.sub.2 S (6)
Phenyl methyl (2,4-F) Benzyl Hexyl (5) Me H S CH NH Bond
CH.sub.2--CH.sub.2 O (6) Phenyl (2,3-F) Isopropyl Isopropyl (5) Me
Et O N S Bond CH.dbd.CH O (6) Phenyl (4-OMe) Isopropyl 2-Propynyl
(5) H H NH CH NMe Bond CH.sub.2--CH.sub.2 O (6) Phenyl 4-Acetyl-
Isopropyl 2-Butenyl (4) H H NMe CH NPr Bond CH.sub.2--CH.sub.2 O
(5) phenyl 4-Cyclo- Isopropyl 2-Pentenyl (4) Me Me S CH O Bond
CH.sub.2--CH.sub.2 S (5) hexylphenyl
(Typical Compounds 5)
The compounds of the formula (I) wherein A is S, X.sup.1 is a bond,
X.sup.2 is a bond, Y is CH.sub.2CH.sub.2, B.sup.1 is N, B.sup.2 is
O, R.sup.1 is a substituent at the 2nd position, R.sup.2 is a
substituent at the 4th position (namely Y is attached to the 5th
position) are shown in Tables 21 and 22.
TABLE-US-00021 TABLE 21 R.sup.3 (position of Z (position of R.sup.1
R.sup.2 substitution) R.sup.4 R.sup.5 substitution)
(4-CF.sub.3)Phenyl Isopropyl Methyl (5) H H O (6)
(4-CF.sub.3)Phenyl Isopropyl Methyl (5) Me Me O (6)
(4-CF.sub.3)Phenyl Isopropyl Methyl (5) H H S (6)
(4-CF.sub.3)Phenyl Isopropyl Methyl (5) H H NH (6)
(4-OCF.sub.3)Phenyl Isopropyl Methyl (5) H H O (6)
(4-OCF.sub.3)Phenyl Isopropyl Methyl (5) Me Me O (6) (4-Cl)Phenyl
Isopropyl Methyl (5) H H O (6) (4-Cl)Phenyl Isopropyl Methyl (5) Me
Me O (6) (4-CF.sub.3)Phenyl Isopropyl Cl (5) H H S (6)
(4-CF.sub.3)Phenyl Isopropyl Cl (5) Me Me O (6) (4-CF.sub.3)Phenyl
Isopropyl F (5) H H S (6) (4-CF.sub.3)Phenyl Isopropyl F (5) Me Me
O (6) (4-CF.sub.3)Phenyl Isopropyl Allyl (5) H H O (6)
(4-CF.sub.3)Phenyl Isopropyl Allyl (5) Me Me O (6)
(4-CF.sub.3)Phenyl Isopropyl Ethyl (5) H H O (6) (4-CF.sub.3)Phenyl
Isopropyl Ethyl (5) Me Me S (6)
TABLE-US-00022 TABLE 22 R.sup.3 (position of Z (position of R.sup.1
R.sup.2 substitution) R.sup.4 R.sup.5 substitution) 4-Pyridyl
Isopropyl Methyl (5) H H O (6) 4-Pyridyl Isopropyl Methyl (5) Me Me
O (6) (4-Me) Isopropyl Methyl (5) H H S (6) Phenyl (4-Me) Isopropyl
Methyl (5) Me Me O (6) Phenyl (4-Me) Hexyl Methyl (5) H H O (6)
Phenyl (4-Me) Hexyl Methyl (5) Me Me O (6) Phenyl (4-CF.sub.3)
Hexyl Methyl (5) H H S (6) Phenyl (4-CF.sub.3) Hexyl Methyl (5) Me
Me O (6) Phenyl (4-CF.sub.3) (4-CF.sub.3) Methyl (5) H H S (6)
Phenyl Phenethyl (4-CF.sub.3) (4-CF.sub.3) Methyl (5) Me Me O (6)
Phenyl Phenethyl (4-CF.sub.3) Sec-butyl Methyl (5) H H O (6) Phenyl
(4-CF.sub.3) Sec-butyl Methyl (5) Me Me O (6) Phenyl (4-CF.sub.3)
Butyl Methyl (5) H H O (6) Phenyl (4-CF.sub.3) Butyl Methyl (5) Me
Me O (6) Phenyl (4-CF.sub.3) Ethyl Methyl (5) H H O (6) Phenyl
(4-CF.sub.3) Methyl Methyl (5) Me Me O (6) Phenyl
(Typical Compounds 6)
The compounds of the formula (I) wherein A is O, R.sup.2 is
isopropyl, X.sup.1 is a bond, X.sup.2 is a bond, Y is
CH.sub.2CH.sub.2, B.sup.1 is N, B.sup.2 is O, R.sup.1 is a
substituent at the 2nd position, R.sup.2 is a substituent at the
5th position (namely Y is attached to the 4th position) are shown
in Table 23.
TABLE-US-00023 TABLE 23 R.sup.3 (position of Z (position of R.sup.1
substitution) R.sup.4 R.sup.5 substitution) (2,4-Cl)Phenyl Methyl
(5) H H O (6) (2,4-Cl)Phenyl Methyl (5) Me Me O (6) (2,4-Cl)Phenyl
Methyl (5) H H S (6) (4-CF.sub.3)Phenyl Methyl (5) H H NH (6)
(4-OCF.sub.3)Phenyl Methyl (5) H H O (6) (4-OCF.sub.3)Phenyl Methyl
(5) Me Me O (6) (4-CF.sub.3)Phenyl Cl (5) H H S (6)
(4-CF.sub.3)Phenyl Cl (5) Me Me O (6) (4-CF.sub.3)Phenyl F (5) H H
S (6) (4-CF.sub.3)Phenyl F (5) Me Me O (6) (4-CF.sub.3)Phenyl Allyl
(5) H H O (6) (4-CF.sub.3)Phenyl Allyl (5) Me Me O (6)
(4-CF.sub.3)Phenyl Ethyl (5) H H O (6) (4-CF.sub.3)Phenyl Ethyl (5)
Me Me S (6)
(Typical Compounds 7)
The compounds of the formula (I) wherein R.sup.3 is methyl, which
is a substituent at the 5th position, X.sup.1 is a bond, X.sup.2 is
a bond, Y is CH.sub.2CH.sub.2, B.sup.1 is N, B.sup.2 is O, R.sup.1
is a substituent at the 2nd position, R.sup.2 is a substituent at
the 5th position (namely Y is attached to the 4th position) are
shown in Table 24.
TABLE-US-00024 TABLE 24 Z (position of R.sup.1 R.sup.2 R.sup.4
R.sup.5 A substitution) 4-Pyridyl Isopropyl H H O O (6) 4-Pyridyl
Isopropyl Me Me O O (6) (4-Me)Phenyl Isopropyl H H O S (6)
(4-Me)Phenyl Isopropyl Me Me O O (6) (4-Me)Phenyl Hexyl H H O O (6)
(4-Me)Phenyl Hexyl Me Me O O (6) (4-CF.sub.3)Phenyl Hexyl H H O S
(6) (4-CF.sub.3)Phenyl Hexyl Me Me O O (6) (4-CF.sub.3)Phenyl
(4-CF.sub.3) H H NH S (6) Phenethyl (4-CF.sub.3)Phenyl (4-CF.sub.3)
Me Me NMe O (6) Phenethyl (2-OH, 4-Cl) Isopropyl H H O O (6) Phenyl
(2-OH, 4-Cl) Isopropyl Me Me O O (6) Phenyl
The pharmacological effects of the invention are described
below.
PPAR.delta. transactivation activity of the compound of the
invention was measured in the manner described below.
A receptor expression plasmid (GAL4-hPPAR.delta. LBD), a reporter
plasmid (UASx4-TK-LUC) and a .beta.-galactosidase (.beta.-GAL)
expression plasmid were transfected into CV-1 cells by using
DMRIE-C reagent. Subsequently, the cells were incubated for 40
hours in the presence of a compound of the invention, and then the
luciferase and .beta.-GAL activities of the cell lysate were
assayed.
The luciferase activity was corrected by the .beta.-GAL activity,
and a relative transactivation activity was calculated under the
condition that the luciferase activity of the cells treated by
L-165041 was regarded as 100% (see the below-mentioned Examples 11
and 12).
As shown in Tables 25 and 26, it is clear that the compounds of the
invention (Examples 1, 2, 5 10) show selective and potent
PPAR.delta. transactivation activities.
Furthermore, it is clear from Table 27 the compound described in
Example 6 show a potent HDL cholesterol elevating effect.
Apparently, the compounds of the invention having the general
formula (I) show potent PPAR.delta. transactivation activities.
Accordingly, these compounds are expected to be useful for
prevention and treatment of the following diseases: hyperglycemia,
diabetes, insulin resistance, dyslipidemia, hyperlipidemia,
obesity, syndrome X, hypercholesterolemia, other dyslipidemia such
as hyperlipopreoteinemia, atherosclerosis, diseases of
cardiovascular systems, hyperphagia, ischemic diseases, malignant
tumors such as lung cancer, mammary cancer, colonic cancer, cancer
of great intestine, and ovary cancer, Alzheimer's disease,
inflammatory disease, osteoporosis (Mano H. et al., (2000) J. Biol.
Chem., 175:8126 8132), Basedow's disease, and adrenal cortical
dystrophy.
The compound of the invention can be administered to human by
ordinary administration methods such as oral administration or
parenteral administration.
The compound can be granulated in ordinary manners for the
preparation of pharmaceuticals. For instance, the compound can be
processed to give pellets, granule, powder, capsule, suspension,
injection, suppository, and the like.
For the preparation of these pharmaceuticals, ordinary additives
such as vehicles, disintegrators, binders, lubricants, dyes, and
diluents. As the vehicles, lactose, D-mannitol, crystalline
cellulose and glucose can be mentioned. Further, there can be
mentioned starch and carboxymethylcellulose calcium (CMC-Ca) as the
disintegrators, magnesium stearate and talc as the lubricants, and
hydroxypropylcellulose (HPC), gelatin and polyvinylpirrolidone
(PVP) as the binders.
The compound of the invention can be administered to an adult
generally in an amount of 0.1 mg to 100 mg a day by parenteral
administration and 1 mg to 2,000 mg a day by oral administration.
The dosage can be adjusted in consideration of age and conditions
of the patient.
The invention is further described by the following non-limiting
examples.
EXAMPLES
Example 1
[[7-Allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2-b-
enzisoxazol-6-yl]oxy]acetic acid
(1)
6-Acetamido-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl-
]-1,2-benzisoxazole
6-Acetamido-3-methyl-1,2-benzisoxazole (571 mg, 3.00 mmol) was
dissolved in dry THF (18 mL). 2M of LDA (3.1 mL, 6.2 mmol) was
dropwise added to the solution for 10 minutes under nitrogen
atmosphere at -78.degree. C. A solution of
4-iodomethyl-5-isopropyl-2-(2,4-dichlorophenyl)oxazole (1.19 g,
3.00 mmol) in THF (3.0 mL) was dropwise added to the resulting
mixture for 7 minutes. The mixture was stirred for 1 hour under the
same conditions. The mixture was allowed to room temperature. A
saturated aqueous ammonium chloride solution and ethyl acetate were
added to the mixture. The organic layer was washed with water and
saline, and dried over anhydrous sodium sulfate. Ethyl acetate was
removed under reduced pressure. The residue was purified by column
chromatography on silica gel with chloroform/methanol (80/1) to
give the desired compound (904 mg) as pale yellow oil (yield
70%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.10 (d, 6H, J=7 Hz),
2.22 (s, 3H), 2.93 (dq, 1H, J=7 Hz, 7 Hz), 3.06 (t, 2H, J=7 Hz),
3.34 (t, 2H, J=7 Hz), 7.12 (dd, 1H, J=2, 9 Hz), 7.32 (dd, 1H, J=2,
9 Hz), 7.37 (d, 1H, J=2 Hz), 7.43 (d, 1H, J=9 Hz), 7.51 (d, 1H, J=2
Hz), 7.89 (d, 1H, J=9 Hz), 8.05 (s, 1H).
(2)
6-Hydroxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]--
1,2-benzisoxazole
The obtained
6-acetamido-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,-
2-benzisoxazole (900 mg, 1.96 mmol) was reacted in 3N hydrochloric
acid (45 mL) at 100.degree. C. for 4 hours. The reaction mixture
was allowed to room temperature, neutralized with a saturated
aqueous sodium hydrogen carbonate solution, and extracted with
ethyl acetate. The organic layer was washed with saline, and dried
over anhydrous sodium sulfate. The solvent was removed under
reduced pressure to give
6-amino-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2-be-
nzisoxazole (770 mg) as brown oil in the residue. The product (770
mg, 1.85 mmol) was suspended in 25% sulfuric acid (9.3 mL), and
cooled with ice. An aqueous sodium nitrite solution (166 mg, 2.41
mmol/1.3 ml) was dropwise added to the suspension for 5 minutes.
The resulting solution was stirred for 45 minutes under the same
conditions. The reaction mixture was dropwise added to a 75%
sulfuric acid (7.4 mL) while refluxing for 5 minutes, and the
mixture was refluxed for 4 hours. The reaction mixture was left to
get cool, extracted with diethyl ether, washed with a saturated
aqueous ammonium chloride solution and saline, and dried over
anhydrous sodium sulfate. Diethyl ether was removed under reduced
pressure. The residue was purified by column chromatography on
silica gel with hexane/ethyl acetate (3/1) to give the desired
compound (138 mg) as pale yellow oil (yield of the two steps
17%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (d, 6H, J=7 Hz),
2.93 (dq, 1H, J=7 Hz, 7 Hz), 3.06 (t, 2H, J=7 Hz), 3.32 (t, 2H, J=7
Hz), 5.36 (s, 1H), 6.75 (dd, 1H, J=2, 8 Hz) 6.92 (d, 1H, J=2 Hz)
7.30 (dd, 1H, J=2, 8 Hz), 7.35 (d, 1H, J=8 Hz), 7.51 (d, 1H, J=2
Hz), 7.89 (d, 1H, J=8 Hz).
(3)
6-Allyloxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-
-1,2-benzisoxazole
The obtained
6-hydroxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2--
benzisoxazole (500 mg, 1.20 mmol) and potassium carbonate (249 mg,
1.80 mmol) were suspended in acetone (30 mL). Allyl bromide (217
mg, 1.80 mmol) was dropwise added to the suspension for 1 minute
while cooling with ice. The mixture was allowed to room
temperature, and stirred for 20 hours. After insoluble was filtered
off, the filter cake was washed with acetone. The filtrate and
washings were combined, and concentrated. The residue was purified
by column chromatography on silica gel with hexane/ethyl acetate
(4/1) to give the desired compound (420 mg) as colorless oil (yield
77%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
2.92 (dq, 1H, J=7 Hz, 7 Hz), 3.06 (t, 2H, J=7 Hz), 3.32 (t, 2H, J=7
Hz), 4.5 4.6 (m, 2H), 5.3 5.5 (m, 2H), 6.0 6.1 (m, 1H), 6.86 (dd,
1H, J=2, 8 Hz) 6.96 (d, 1H, J=2 Hz) 7.32 (dd, 1H, J=2, 8 Hz), 7.36
(d, 1H, J=8 Hz), 7.51 (d, 1H, J=2 Hz), 7.90 (d, 1H, J=8 Hz).
(4)
7-Allyl-6-hydroxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl-
]ethyl]-1,2-benzisoxazole
6-Allyloxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2-
-benzisoxazole (70 mg, 0.153 mmol) was heated at 180.degree. C. for
4 hours. The product was allowed to room temperature, and purified
by column chromatography on silica gel with hexane/ethyl acetate
(3/1) to give the desired compound (47 mg) as white powder (yield
67%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
2.92 (dq, 1H, J=7 Hz, 7 Hz), 3.05 (t, 2H, J=7 Hz), 3.31 (t, 2H, J=7
Hz), 3.65 3.70 (m, 2H), 5.15 5.25 (m, 2H), 5.41 (s, 1H), 6.0 6.1
(m, 1H), 6.76 (d, 1H, J=8 Hz), 7.25 (d, 1H, J=8 Hz) 7.32 (dd, 1H,
J=2, 8 Hz), 7.51 (d, 1H, J=2 Hz), 7.89 (d, 1H, J=8 Hz).
(5) Ethyl
[[7-allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]e-
thyl]-1,2-benzisoxazol-6-yl]oxy]acetate
The obtained
7-allyl-6-hydroxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]eth-
yl]-1,2-benzisoxazole (45 mg, 0.098 mmol) and potassium carbonate
(20 mg, 0.147 mmol) was suspended in acetone (5.0 mL). An acetone
solution of ethyl bromoacetate (25 mg, 0.147 mmol) was added to the
suspension while cooling with ice. The mixture was allowed to room
temperature, and stirred for 20 hours. After insoluble was filtered
off, the filter cake was washed with acetone. The filtrate and
washings were combined, and concentrated. The residue was purified
by column chromatography on silica gel with hexane/ethyl acetate
(4/1) to give the desired compound (43 mg) as colorless oil (yield
80%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
1.28 (t, 3H, J=7 Hz), 2.91 (dq, 1H, J=7 Hz, 7 Hz), 3.05 (t, 2H, J=7
Hz), 3.32 (t, 2H, J=7 Hz), 3.65 3.70 (m, 2H), 4.25 (q, 2H, J=7 Hz)
4.70 (s, 2H) 4.95 5.15 (m, 2H), 6.0 6.1 (m, 1H), 6.74 (d, 1H, J=9
Hz), 7.30 (d, 1H, J=9 Hz) 7.32 (dd, 1H, J=2, 9 Hz), 7.51 (d, 1H,
J=2 Hz), 7.89 (d, 1H, J=9 Hz).
(6)
[[7-Allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]--
1,2-benzisoxazol-6-yl]oxy]acetic acid
The obtained ester compound (40 mg, 0.074 mmol) was dissolved in an
ethanol-water (3.0 mL 1.5 mL). Lithium hydroxide Monohydrate of (8
mg) was added to the solution, and the mixture was stirred for 20
hours. Ice was added to the reaction mixture. The mixture was
neutralized with 3N hydrochloric acid. The crystals were filtered,
washed with water, air-dried over night, and further dried under
reduced pressure to give the desired compound (25 mg) as white
powder (yield 66%). mp 80 85.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.13 (d, 6H, J=7 Hz),
2.94 (dq, 1H, J=7 Hz, 7 Hz), 3.06 (t, 2H, J=7 Hz), 3.31 (t, 2H, J=7
Hz), 3.65 3.75 (m, 2H), 4.73 (s, 2H) 5.0 5.2 (m, 2H), 5.9 6.1 (m,
1H), 6.76 (d, 1H, J=9 Hz), 7.32 (d, 1H, J=9 Hz) 7.32 (dd, 1H, J=2,
9 Hz), 7.51 (d, 1H, J=2 Hz), 7.89 (d, 1H, J=9 Hz).
Example 2
2-[[7-Allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2-
-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
(1) Ethyl
2-[[7-allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl-
]ethyl]-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionate
7-Allyl-6-hydroxy-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]eth-
yl]-1,2-benzisoxazole (92 mg, 0.20 mmol), ethyl
2-bromo-2-methylpropionate (196 mg, 1.00 mmol) and potassium
carbonate (138 mg, 1.00 mmol) were suspended in methyl ethyl ketone
(5.0 mL). The suspension was refluxed for 20 hours, and allowed to
room temperature. After insoluble was filtered off, the filter cake
was washed with methyl ethyl ketone. The filtrate and washings were
combined, and concentrated. The residue was purified by column
chromatography on silica gel with hexane/ethyl acetate (5/1) to
give the desired compound (100 mg) as colorless oil (quantitative
yield).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.09 (d, 6H, J=7 Hz),
1.23 (t, 3H, J=7 Hz), 1.60 (s, 6H), 2.90 (dq, 1H, J=7 Hz, 7 Hz),
3.04 (t, 2H, J=7 Hz), 3.31 (t, 2H, J=7 Hz), 3.65 3.70 (m, 2H), 4.23
(q, 2H, J=7 Hz), 4.95 5.15 (m, 2H), 6.0 6.1 (m, 1H), 6.65 (d, 1H,
J=9 Hz), 7.19 (d, 1H, J=9 Hz), 7.32 (dd, 1H, J=2, 9 Hz), 7.51 (d,
1H, J=2 Hz), 7.89 (d, 1H, J=9 Hz).
(2)
2-[[7-Allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl-
]-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
The desired compound was obtained in an analogous manner as in (6)
of Example 1 (yield 73%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.10 (d, 6H, J=7 Hz),
1.63 (s, 6H), 2.92 (dq, 1H, J=7 Hz, 7 Hz), 3.06 (t, 2H, J=7 Hz),
3.31 (t, 2H, J=7 Hz), 3.65 3.70 (m, 2H), 5.00 5.15 (m, 2H), 5.9 6.1
(m, 1H), 6.80 (d, 1H, J=8 Hz), 7.21 (d, 1H, J=8 Hz), 7.30 (dd, 1H,
J=2, 8 Hz), 7.49 (d, 1H, J=2 Hz), 7.85 (d, 1H, J=8 Hz).
Example 3
[[7-Propyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-1,2--
benzisoxazol-6-yl]oxy]acetic acid
(1) Ethyl
[[7-propyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]-
ethyl]-1,2-benzisoxazol-6-yl]oxy]acetate
Ethyl
[[7-allyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl-
]-1,2-benzisoxazol-6-yl]oxy]acetate (65 mg, 0.12 mmol) was
dissolved in ethanol (7.0 mL). To the solution, 10% Pd--C (6 mg)
was added. The mixture was stirred for 3 hours under hydrogen
atmosphere (and ordinary pressure). After insoluble was filtered
off, the filtrate was condensed to give the desired compound (63
mg) as colorless oil in the residue (yield 97%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 0.97 (t, 3H, J=7 Hz),
1.09 (d, 6H, J=7 Hz), 1.26 (t, 3H, J=7 Hz), 1.7 1.8 (m, 2H), 2.9
3.0 (m, 3H), 3.05 (t, 2H, J=7 Hz), 3.32 (t, 2H, J=7 Hz), 4.25 (q,
2H, J=7 Hz), 4.70 (s, 2H), 6.71 (d, 1H, J=8 Hz), 7.25 (d, 1H, J=9
Hz), 7.33 (dd, 1H, J=2, 9 Hz), 7.51 (d, 1H, J=2 Hz), 7.89 (d, 1H,
J=8 Hz).
(2)
[[7-Propyl-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-
-1,2-benzisoxazol-6-yl]oxy]acetic acid
The desired compound was obtained in an analogous manner as in (6)
of Example 1.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 0.96 (t, 3H, J=7 Hz),
1.12 (d, 6H, J=7 Hz), 1.7 1.8 (m, 2H), 2.9 3.0 (m, 3H), 3.06 (t,
2H, J=7 Hz), 3.30 (t, 2H, J=7 Hz), 4.25 (q, 2H, J=7 Hz), 4.74 (s,
2H), 6.74 (d, 1H, J=8 Hz), 7.26 (d, 1H, J=9 Hz), 7.33 (dd, 1H, J=2,
9 Hz), 7.51 (d, 1H, J=2 Hz), 7.87 (d, 1H, J=8 Hz).
Example 4
2-[[7-Allyl-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazolyl]et-
hyl]-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
The following (1) to (5) were conducted in an analogous manner as
in Example 1, and the following (6) and (7) were conducted in an
analogous manner as in Example 2.
(1)
6-Acetamido-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazol-
yl]ethyl]-1,2-benzisoxazole
Pale yellow oil
Yield 20%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
2.23 (s, 3H), 3.03 (dq, 1H, J=7 Hz, 7 Hz), 3.25 3.40 (m, 4H), 7.20
(dd, 1H, J=2, 9 Hz), 7.36 (d, 1H, J=2 Hz), 7.44 (d, 1H, J=9 Hz),
7.65 (d, 2H, J=8 Hz), 7.99 (d, 2H, J=8 Hz), 8.07 (s, 1H).
(2)
6-Amino-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazolyl]e-
thyl]-1,2-benzisoxazole
Pale yellow oil
Yield 93%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
3.04 (dq, 1H, J=7 Hz, 7 Hz), 3.15 3.40 (m, 4H), 4.0 4.05 (br, 2H),
6.61 (dd, 1H, J=2, 9 Hz), 6.73 (d, 1H, J=2 Hz), 7.27 (d, 1H, J=9
Hz), 7.65 (d, 2H, J=8 Hz), 8.00 (d, 2H, J=8 Hz),
(3)
6-Hydroxy-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazolyl-
]ethyl]-1,2-benzisoxazole
Pale yellow oil
Yield 32%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
3.03 (dq, 1H, J=7 Hz, 7 Hz), 3.2 3.4 (m, 4H), 5,95 6.00 (br, 1H),
6.83 (dd, 1H, J=2, 9 Hz), 6.79 (d, 1H, J=2 Hz), 7.38 (d, 1H, J=9
Hz), 7.64 (d, 2H, J=8 Hz), 7.99 (d, 2H, J=8 Hz).
(4)
6-Allyloxy-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazoly-
l]ethyl]-1,2-benzisoxazole
Pale yellow oil
Yield 49%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
3.04 (dq, 1H, J=7 Hz, 7 Hz), 3.2 3.4 (m, 4H), 4.60 4.65 (m, 2H),
5.30 5.50 (m, 2H), 6.00 6.15 (m, 1H), 6.93 (dd, 1H, J=2, 9 Hz),
7.00 (d, 1H, J=2 Hz), 7.40 (d, 1H, J=9 Hz), 7.65 (d, 2H, J=8 Hz),
8.00 (d, 2H, J=8 Hz).
(5)
7-Allyloxy-6-hydroxy-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl--
5-thiazolyl]ethyl]-1,2-benzisoxazole
White powder
Yield 71%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
3.03 (dq, 1H, J=7 Hz, 7 Hz), 3.2 3.4 (m, 4H), 3.65 3.75 (m, 2H),
5.15 5.30 (m, 2H), 5.56 (s, 1H), 6.00 6.10 (m, 1H), 6.84 (d, 1H,
J=9 Hz), 7.28 (d, 1H, J=9 Hz), 7.65 (d, 2H, J=8 Hz), 8.00 (d, 2H,
J=8 Hz).
(6) Ethyl
2-[[7-allyl-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-t-
hiazolyl]ethyl]-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionate
Pale yellow oil
Yield 48%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (t, 3H, J=7 Hz),
1.23 (d, 6H, J=7 Hz), 1.62 (s, 6H), 3.02 (dq, 1H, J=7 Hz, 7 Hz),
3.2 3.4 (m, 4H), 3.65 3.75 (m, 2H), 4.23 (q, 2H, J=7 Hz), 5.0 5.15
(m, 2H), 5.95 6.10 (m, 1H), 6.71 (d, 1H, J=9 Hz), 7.22 (d, 1H, J=9
Hz), 7.65 (d, 2H, J=8 Hz), 7.99 (d, 2H, J=8 Hz).
(7)
2-[[7-Allyl-3-[2-[2-[(4-trifluoromethyl)phenyl]-4-isopropyl-5-thiazol-
yl]ethyl]-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
Colorless oil
Yield 81%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (d, 6H, J=7 Hz),
1.65 (s, 6H), 3.01 (dq, 1H, J=7 Hz, 7 Hz), 3.2 3.4 (m, 4H), 3.65
3.75 (m, 2H), 5.0 5.15 (m, 2H), 5.95 6.10 (m, 1H), 6.85 (d, 1H, J=9
Hz), 7.27 (d, 1H, J=9 Hz), 7.65 (d, 2H, J=8 Hz), 7.99 (d, 2H, J=8
Hz).
Example 5
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]-ethyl]-5-methy-
l-1,2-benzisoxazol-6-yl]oxyacetic acid
(1)
6-Acetamido-3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl-
]ethyl]-5-methyl-1,2-benzisoxazole
6-Acetamido-3,5-dimethyl-1,2-benzisoxazole (9.18 g, 45.0 mmol) was
dissolved in dry THF (315 mL). To the solution, 2M LDA (53 mL,
106.0 mmol) was dropwise added for 40 minutes at -78.degree. C.
under nitrogen atmosphere, and the mixture was stirred for 15
minutes at the same conditions (at -78.degree. C.), to which THF
solution (100 mL) of
4-isopropyl-5-iodomethyl-2-(4-trifluoromethyl)phenylthiazole (18.51
g, 45.0 mmol) was dropwise added for 45 minutes. The mixture was
stirred for 1 hour under the same conditions (at -78.degree. C.),
and allowed to room temperature. A saturated aqueous ammonium
chloride solution and ethyl acetate were added to reaction the
mixture. The ethyl acetate layer was washed with water and saline,
dried over anhydrous sodium sulfate. After ethyl acetate was
removed, the residue was purified by column chromatography on
silica gel with hexane/ethyl acetate (1/1) to give the desired
compound (7.40 g) as pale yellow crystals (yield 34%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
2.26 (bs, 3H), 2.32 (s, 3H), 3.04 (m, 1H), 3.26 (dd, 2H, J=6, 8
Hz), 3.37 (dd, 2H, J=6, 8 Hz), 7.12 (bs, 1H), 7.65 (d, 2H, J=8 Hz),
7.99 (d, 2H, J=8 Hz), 8.40 (bs, 1H).
(2)
6-Amino-3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]eth-
yl]-5-methyl-1,2-benzisoxazole
The obtained amide compound (18.0 g, 36.9 mmol) was suspended in 4M
hydrochloric acid (360 mL) and acetic acid (180 mL). The suspension
was refluxed for 24 hours, allowed to room temperature, poured in
to ice-cold water, and neutralized with 10N aqueous sodium
hydroxide solution. After ethyl acetate was added to the mixture,
the organic layer was washed with saline, dried over anhydrous
sodium sulfate. After the solvent was removed, the crude crystal in
the residue was filtered, washed with hexane to give the desired
compound (16.8 g) as pale brown crystal (yield 94%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
2.21 (s, 3H), 3.05 (m, 1H), 3.21 (dd, 2H, J=6, 9 Hz), 3.35 (dd, 2H,
J=6, 9 Hz), 4.01 (bs, 2H), 6.75 (s, 1H), 7.14 (s, 1H), 7.64 (d, 2H,
J=8 Hz), 8.00 (d, 2H, J=8 Hz).
(3)
6-Hydroxy-3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]e-
thyl]-5-methyl-1,2-benzisoxazole
The obtained amine compound (15.4 g, 34.6 mmol) was suspended in
25% sulfuric acid (170 ml). An aqueous sodium nitrite solution
(3.10 g, 45 mmol) was added to the suspension while cooling with
ice. The mixture was stirred for 20 minutes under the same
conditions, and dropwise added to 75% sulfuric acid heated at
130.degree. C. The mixture was refluxed for 3 hours under the same
conditions, allowed to room temperature, and poured into ice-cold
water. After ethyl acetate were added to the mixture, the organic
layer was dried over anhydrous sodium sulfate. After the solvent
was removed, the crude crystal in the residue was filtered, and
washed with hexane to give the desired compound (8.36 g) as pale
brown crystals (yield 54%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
2.30 (s, 3H), 3.04 (m, 1H), 3.2 3.4 (m, 4H), 5.31 (s, 1H), 6.93 (s,
1H), 7.22 (s, 1H), 7.65 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
(4) Ethyl
[3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethy-
l]-5-methyl-1,2-benzisoxazol-6-yl]oxyacetate
The desired compound was obtained in an analogous manner as in (5)
of Example 1.
White powder
Yield 65%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
1.31 (t, 3H, J=7 Hz), 2.33 (s, 3H), 3.04(m, 1H), 3.2 3.4(m, 4H),
4.12(q, 2H, J=7 Hz) 4.71 (s, 2H), 6.83 (s, 1H), 7.25 (s, 1H), 7.65
(d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
(5)
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-m-
ethyl-1,2-benzisoxazol-6-yl]oxyacetic acid
The desired compound was obtained in an analogous manner as in (6)
of Example 1.
White powder
Yield 71%
mp (dec) 180 190.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
2.33 (s, 3H), 3.03 (m, 1H), 3.2 3.4 (m, 4H), 4.77 (s, 2H), 6.87 (s,
1H), 7.26 (s, 1H), 7.64 (d, 2H, J=9 Hz), 7.99 (d, 2H, J=9 Hz).
IR(KBr)cm.sup.-1: 2960, 2930, 1740, 1620, 1520, 1450, 1420, 1320,
1280, 1250, 1160, 1120, 1060, 840.
Example 6
2-[[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-met-
hyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
(1) Ethyl
2-[[3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]e-
thyl]-5-methyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionate
The desired compound was obtained in an analogous manner as in (1)
of Example 2.
Pale yellow oil
Yield 85%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (d, 6H, J=7 Hz),
1.24 (t, 3H, J=7 Hz), 1.67 (s, 6H) 2.26 (s, 3H), 3.02 (m, 1H), 3.2
3.4 (m, 4H), 4.25 (q, 2H, J=7 Hz) 6.77 (s, 1H), 7.25 (s, 1H), 7.65
(d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
(2)
2-[[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]--
5-methyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
The desired compound was obtained in an analogous manner as in (2)
of Example 2.
White crystal
Yield 94%
mp (dec) 166 168.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (d, 6H, J=7 Hz),
1.72 (s, 6H) 2.28 (s, 3H), 3.02 (m, 1H), 3.2 3.4 (m, 4H), 6.93 (s,
1H), 7.25 (s, 1H), 7.65 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
IR(KBr)cm.sup.-1: 3000, 1720, 1620, 1520, 1450, 1370, 1320, 1280,
1160, 1120, 1060, 850, 820.
Example 7
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-methyl-
-1,2-benzisoxazol-6-yl]thioacetic acid
(1)
3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]-ethyl]-6-m-
ercapto5-methyl-1,2-benzisoxazole
6-Amino-3-[2-[isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5--
methyl-1,2-benzisoxazole (475 mg, 1.07 mmol) was dissolved in
ethanol (3.3 mL). Concentrated hydrochloric acid (1.68 mL) was
added to the solution while cooling with ice (external temperature
0.degree. C.). Sodium nitrite (81 mg, 1.17 mmol) was added to the
mixture. An aqueous potassium xanthogenate solution (430 mg, 2.68
mmol) was dropwise added to the mixture for 5 minutes. The
interreact temperature was kept at 45.degree. C., and the mixture
was stirred for 18 hours. The mixture was left to get cool, poured
into water, and extracted with ethyl acetate. The organic layer was
washed with saline, dried over anhydrous sodium sulfate. After the
solvent was removed, the residue was purified by column
chromatography on silica gel with hexane/ethyl acetate (5/1) to
give crude dithiocarbonic ester compound (131 mg). The
dithiocarbonic ester compound was dissolved in ethanol. An aqueous
solution of sodium hydroxide (36 mg) was added to the solution at
room temperature. The mixture was refluxed for 3 hours. The
reaction mixture was poured into ice-cold water, and neutralized
with 1 M hydrochloric acid. After ethyl acetate was added to the
mixture, the organic layer was washed with saline, dried over
anhydrous sodium sulfate. After the solvent was removed, the
residue was purified by column chromatography on silica gel with
hexane/ethyl acetate (5/1) to give the desired compound (65 mg)
(yield of the two steps 7%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.23 (d, 6H, J=7 Hz),
2.38 (s, 3H), 3.04 (m, 1H), 3.2 3.4 (m, 4H), 3.60 (s, 1H), 7.26 (s,
1H), 7.49 (s, 1H) 7.65 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
(2) Ethyl
[[3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]eth-
yl]-5-methyl-1,2-benzisoxazol-6-yl]thio]acetate
The desired compound was obtained in an analogous manner as in (5)
of Example 1.
Pale yellow oil
Yield 69%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
1.27 (t, 3H, J=7 Hz), 2.41 (s, 3H), 3.03 (m, 1H), 3.2 3.4 (m, 4H),
3.76 (s, 2H), 4.22(q, 2H, J=7 Hz) 7.27 (s, 1H), 7.45 (s, 1H), 7.65
(d, 2H, J=9 Hz), 7.99 (d, 2H, J=9 Hz).
(3)
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-m-
ethyl-1,2-benzisoxazol-6-yl]thioacetic acid
The desired compound was obtained in an analogous manner as in (6)
of Example 1.
Pale yellowish white powder
Yield 73%
mp (dec) 170.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.24 (d, 6H, J=7 Hz),
2.42 (s, 3H), 3.03 (m, 1H), 3.2 3.4 (m, 4H), 3.81 (s, 2H), 7.28 (s,
1H), 7.46 (s, 1H), 7.65 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
Example 8
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-methyl-
-1,2-benzisoxazol-6-yl]aminoacetic acid
(1) Ethyl
[3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethy-
l]-5-methyl-1,2-benzisoxazol-6-yl]aminoacetate
6-Amino-3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]--
5-methyl-1,2-benzisoxazole (445 mg, 1.00 mmol), ethyl bromoacetate
(154 mg, 1.20 mmol), diisopropylethylamine (142 mg, 1.10 mmol) were
dissolved in DMF (10 mL). The solution was refluxed for 18 hours,
poured into water, and extracted with ethyl acetate. The organic
layer was washed with saline, dried over anhydrous sodium sulfate.
After the solvent was removed, the residue was purified by column
chromatography on silica gel with hexane/ethyl acetate (3/1) to
give the desired compound (160 mg) as a dark brown powder (yield
30%).
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
1.34 (t, 3H, J=7 Hz), 2.25 (s, 3H), 3.05 (m, 1H), 3.2 3.3 (m, 4H),
3.97 (d, 2H, J=3 Hz) 4.6 4.7 (br, 1H) 4.12 (q, 2H, J=7 Hz) 6.51 (s,
1H), 7.15 (s, 1H), 7.64 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
(2)
[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-m-
ethyl-1,2-benzisoxazol-6-yl]aminoacetic acid
The desired compound was obtained in an analogous manner as in (6)
of Example 1.
Pale brown crystal
Yield 73%
mp (dec) 180 185.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.25 (d, 6H, J=7 Hz),
2.25 (s, 3H), 3.04 (m, 1H), 3.2 3.3 (m, 4H), 4.07 (s, 2H), 6.54 (s,
1H), 7.15 (s, 1H), 7.64 (d, 2H, J=9 Hz), 7.99 (d, 2H, J=9 Hz).
IR(KBr)cm.sup.-1: 3425, 2950, 2925, 1740, 1620, 1520, 1440, 1380,
1370, 1320, 1220, 1160, 1060, 840.
Example 9
[3-[2-[2-(2,4-Dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-5-methyl-1,2-b-
enzisoxazol-6-yl]oxyacetic acid
(1)
6-Acetamido-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl-
]-5-methyl-1,2-benzisoxazole
The desired compound was obtained in an analogous manner as in (1)
of Example 5.
Brown oil
Yield 34%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.10 (d, 6H, J=7 Hz),
2.24 (bs, 3H), 2.26 (s, 3H), 2.92 (m, 1H), 3.05 (t, 2H, J=7 Hz),
3.33 (t, 2H, J=7 Hz), 7.16 (bs, 1H), 7.28 (s, 1H), 7.32 (dd, 1H,
J=2, 9 Hz), 7.51 (d, 1H, J=2 Hz), 7.91 (d, 1H, J=9 Hz), 8.34 (bs,
1H).
(2)
6-Amino-3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-5--
methyl-1,2-benzisoxazole
The desired compound was obtained in an analogous manner as in (2)
of Example 5.
Yield 42%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.10 (d, 6H, J=7 Hz),
2.14 (s, 3H), 2.92 (m, 1H), 3.03 (t, 2H, J=7 Hz), 3.27 (t, 2H, J=7
Hz), 3.97 (bs, 2H), 6.72 (s, 1H), 7.13 (s, 1H), 7.32 (dd, 1H, J=2,
8 Hz), 7.51 (d, 1H, J=2 Hz), 7.92 (d, 1H, J=8 Hz).
(3)
3-[2-[2-(2,4-Dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-6-hydroxy--
5-methyl-1,2-benzisoxazole
The desired compound was obtained in an analogous manner as in (3)
of Example 5.
Pale yellow crystal
Yield 44%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
2.22 (s, 3H), 2.92 (m, 1H), 3.05 (t, 2H, J=7 Hz), 3.29 (t, 2H, J=7
Hz), 6.15 (bs, 1H), 6.88 (s, 1H), 7.19 (s, 1H), 7.32 (dd, 1H, J=2,
9 Hz), 7.51 (d, 1H, J=2 Hz), 7.90 (d, 1H, J=9 Hz).
(4) Ethyl
[[3-[2-[2-(2,4-dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-5--
methyl-1,2-benzisoxazol-6-yl]oxyacetate
The desired compound was obtained in an analogous manner as in (4)
of Example 5.
Yield 97%
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
1.30 (t, 3H, J=7 Hz), 2.23 (s, 3H), 2.91 (m, 1H), 3.04 (t, 2H, J=7
Hz), 3.31 (t, 2H, J=7 Hz), 4.28 (q, 2H, J=7 Hz), 4.69 (s, 2H), 6.80
(s, 1H), 7.24 (s, 1H), 7.33 (dd, 1H, J=2, 8 Hz), 7.51 (d, 1H, J=2
Hz), 7.91 (d, 1H, J=8 Hz).
(5)
[3-[2-[2-(2,4-Dichlorophenyl)-5-isopropyl-4-oxazolyl]ethyl]-5-methyl--
1,2-benzisoxazol-6-yl]oxyacetic acid
The desired compound was obtained in an analogous manner as in (5)
of Example 5.
Yield 88% Pale yellow crystal
mp (dec) 180 184.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: 1.11 (d, 6H, J=7 Hz),
2.26 (s, 3H), 2.93 (m, 1H), 3.04 (t, 2H, J=7 Hz), 3.30 (t, 2H, J=7
Hz), 4.76 (s, 2H) 6.84 (s, 1H), 7.23 (s, 1H), 7.33 (dd, 1H, J=2, 8
Hz), 7.64 (d, 1H, J=2 Hz), 7.99 (d, 1H, =8 Hz).
IR(KBr)cm.sup.-1:1749, 1718, 1625, 1562, 1521, 1457, 1446, 1429,
1388, 1361, 1317, 1284, 1251, 1162, 1103, 1087, 1041, 898, 863,
831, 817, 775, 732, 674, 667, 611.
Example 10
2-[[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-met-
hyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
2-piperidin-1-yl ethyl ester hydrochloride
2-[[3-[2-[4-Isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-me-
thyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid (247 mg,
0.463 mmol) was dissolved in dichloromethane (10.0 mL). To the
solution, oxalyl chloride (0.053 mL, 0.60 mmol) and DMF (1-drop)
were added while cooling with ice. The mixture was allowed to room
temperature, and stirred for 3 hours under nitrogen atmosphere. The
solvent was removed under reduced pressure. The acid chloride
compound in the residue was dissolved in dichloromethane (10.0 mL).
To the solution, piperidine-2-ethanol (90 mg, 0.70 mmol),
triethylamine (71 mg, 0.70 mmol) and 4-dimethylaminopyridine (3 mg)
were added. The mixture was stirred for 20 hours at room
temperature, and poured into ice-cold water. After ethyl acetate
was added to the mixture, the organic layer was washed with saline,
and then with water, dried over anhydrous sodium sulfate. After the
solvent was removed under reduced pressure, the residue was
purified by column chromatography on silica gel with hexane/ethyl
acetate (3/1) to give
2-[[3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]-ethyl]-5-m-
ethyl-1,2-benzisoxazol-6-yl]oxy]-2-methylpropionic acid
2-piperidin-1-yl ethyl ester (190 mg) as colorless oil. The ester
compound was dissolved in diethyl ether (4.0 mL). To the solution,
hydrogen chloride-diethyl ether solution was added. Crystals were
filtered to give the desired compound (160 mg) as white crystal
(yield 51%).
mp 75 80.degree. C.
.sup.1H-NMR (CDCl.sub.3, 400 MHz) .delta.: (Free) 1.24 (d, 6H, J=7
Hz), 1.3 1.4 (m, 2H), 1.5 1.6 (m, 4H) 1.68 (s, 6H), 2.26 (s, 3H),
2.3 2.4 (m, 4H), 2.55 (t, 2H, J=6 Hz), 3.04 (m, 1H), 3.23 (t, 2H,
J=8 Hz), 3.34 (t, 2H, J=8 Hz), 4.31 (t, 2H, J=6 Hz), 6.86 (s, 1H),
7.23 (s, 1H), 7.65 (d, 2H, J=9 Hz), 8.00 (d, 2H, J=9 Hz).
Example 11
Pharmacological Experiment 1
(1) Measurement of PPAR.alpha., .gamma., .delta. transactivation
activity
PPAR.alpha., .gamma., .delta. transactivation activity of each
compound [Examples 1 4] was measured in the manner described
below.
I. Method
1) Material
CV-1 cells were obtained from Tohoku University Aging Medical
Laboratory, Medical Cell Collection Center. All test compounds were
dissolved in dimethylsulfoxide (DMSO). Final concentration of DMSO
was 0.1%.
2) Plasmid
Receptor expression plasmid (GAL4-hPPAR.alpha., LBD
GAL4-hPPAR.gamma.LBD, GAL4-hPPAR.delta. LBD), Reporter plasmid
(UASx4-TK-LUC), and .beta.-galactosidase expression plasmid
(.beta.GAL) similar to Kliewer, S. A., et al., ((1992) Nature,
358:771 774) were used.
3) Transfection
CV-1 cells were seeded in 24 well culture plates at
2.times.10.sup.5 cells per well, and cultured for 24 hours OPTI-MEM
I Reduced Serum Medium (Life Technologies, 500 .mu.L/well)
containing 4%-fetal bovine serum (FBS). After washing with
OPTI-MEM, transfection mixture (250 .mu.L/well) containing 0.03
.mu.g of GAL4-hPPAR.delta. LBD, 0.25 .mu.g of UASx4-TK-LUC, 0.35
.mu.g of .beta.GAL, and 2 .mu.L of lipofection reagent, DMRIE-C
(Life Technologies) were added. The cells were incubated for 5
hours at 37.degree. C.
4) Cell Treatment by Addition of Test Compound
The cells were washed and incubated for 40 hours in the presence of
the test compound (final concentration was 10.sup.-6M).
5) Measurement of the Level of Reporter Gene Expression
The culture medium was removed and the cells were washed with PBS
twice. A solubilizing buffer (100 .mu.L/well) containing 25 mM
Tris-PO.sub.4 (pH 7.8), 15% v/v glycerol, 2% CHAPS, 1% Lecithin, 1%
BSA, 4 mM EGTA (pH 8.0), 8 mM MgCl.sub.2, 1 mM DTT was added. After
the incubation for 10 minutes at room temperature, a portion (20
.mu.L) of the solution was transferred into a 96-well plate.
Subsequently, 100 .mu.L of luciferase substrate solution
(Piccagene: available from Nippon Gene Co., Ltd.) was added, and a
luminous intensity per one second (luciferase activety) was
measured using a microluminoreader (Type MLR-100, Corona Electrics
Co., Ltd.). Each luciferase activety was corrected by the
transfection efficiency which was calculated from
.beta.-galactosidase activity. The assay method of
.beta.-galactosidase activity was as follows: A portion (50 .mu.L)
of the solubilized sample was transferred into another 96-well
plate; 100 .mu.L of ONPG (2-nitrophenyl-.beta.-galactopyranoside)
solution was added and incubated for 5 minutes at room temperature.
50 .mu.L of a reaction stopping solution (1M sodium carbonate
solution) was added. Then the absorbance at 414 nm was
measured.
A relative PPAR activity was calculated as follows: 0% (luciferase
activity of cells treated with DMSO (0.1%) alone), 100% (luciferase
activity of cells treated with a control (PPAR.alpha.: 10.sup.-4 M
WY-165041, PPAR.gamma.: 10.sup.-5 M Rosiglitazone, PPAR.delta.:
10.sup.-4 M L-165041))
II. Results
The results are shown in Table 25.
TABLE-US-00025 TABLE 25 .alpha. .gamma. .delta. Example 1 0.1 9.4
76.9 Example 2 8.0 3.2 67.8 Example 4 85.0 9.5 59.9
It is clear from Table 25 that the compounds of Examples have
potent PPAR.delta. transactivation activity. Particularly, the
compounds of Examples 1 and 2 show selective PPAR.delta.
transactivation activity.
Example 52
Pharmacological Tests 2
PPAR transactivation activities of the compounds of Examples 5 10
were assayed in the same manner as described in Example 11. The
results are shown in Table 26.
TABLE-US-00026 TABLE 26 Test compound .alpha. .gamma. .delta.
Example 5 0 0 75 Example 6 0 0 54 Example 7 0 0 63 Example 8 0 0 61
Example 9 0 2 86 Example 10 3 0 75 GW-2433 64 7 52 GW-501516 0 1
90
Relative activities for PPAR transactivation were shown.
Each value represents as % of control. Cells were cultured in the
presence of compounds at 10.sup.-7 M except that PPAR.delta. of
Examples 5, 6 and 7 were assayed at 10.sup.-8 M.
Positive Control:
.alpha.: 10.sup.-4 M WY-14643 .gamma.: 10.sup.-5 M Rosiglitazone
.delta.: 10.sup.-4 M L-165041
It is clear from Table 26 that the compounds of Examples 6 10 have
potent and selective PPAR.delta. transactivation activities.
Example 13
Pharmacological Experiment 2
HDL Cholesterol Elevating Effect
I. Method
HDL cholesterol elevating effect was measured by using db/db mice,
which are hereditary obesity mice. The db/db mice (10 weeks old)
were divided into groups based on serum HDL cholesterol levels.
Each of the compounds of the present invention (compounds
synthesized in Examples 4 and 10) and GW-501516 was orally
administered for one week twice daily. Mice of the control group
(to which no agent was administered) were orally given 1% methyl
cellulose solution. After 16 hours from the final administration,
blood sample was collected, and serum HDL cholesterol level was
measured. HDL cholesterol was separated by electrophoresis on
agarose gels (Chol/Trig Combo, Helena Laboratories). Serum total
cholesterol levels were measured enzymaticallly using a kit (Pure
Auto, Daiichi Chemicals) by an automatic analyzer (7060E type,
Hitachi Ltd.). HDL cholesterol levels were calculated from total
cholesterol levels and HDL cholesterol/total cholesterol
ratios.
II. Results
Serum HDL cholesterol levels of experiments groups are shown in
Table 27. Each value represents as % of the control group.
TABLE-US-00027 TABLE 27 Test Dose Ratio of increasing HDL compound
(mg/kg/b.i.d.) cholesterol (% to control) Example 6 10 164
GW-501516 10 149
As shown in Table 6, the compound obtained in Example 6 has a
potent HDL cholesterol elevating effect.
* * * * *