U.S. patent number [Application Number ] was granted by the patent office on 0000-00-00 for .
United States Patent |
3,815,790 |
Allen , et al. |
June 11, 1974 |
PRECISION LIQUID PIPETTING DEVICES
Abstract
Precision liquid pipetting devices for use with disposable
pipettes having improved means for adjusting the stroke of the
plunger within the pipette and for precisely calibrating the
volumetric capacity.
Inventors: |
Allen; Donald M. (Pinole,
CA), Lee; Thomas E. (Lafayette, CA) |
Assignee: |
Kensington Scientific
Corporation (Emeryville, CA)
|
Family
ID: |
25768028 |
Appl.
No.: |
05/289,996 |
Filed: |
September 18, 1972 |
Current U.S.
Class: |
73/864.13;
73/864.14; 222/386; 604/208; 422/925; 222/309 |
Current CPC
Class: |
B01L
3/0224 (20130101); G01F 11/06 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); G01F 11/06 (20060101); G01F
11/02 (20060101); G01f 013/00 () |
Field of
Search: |
;222/308,309,386
;73/425.4P,425.6 ;128/218P,218PA,218C,218D,218F,234 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Reeves; Robert B.
Assistant Examiner: Kocovsky; Thomas E.
Claims
We claim:
1. In a precision liquid pipetting device having an elongated
barrel,
a pipette mounted upon one end of the barrel, and
a plunger carried in part within the barrel and reciprocable with a
predetermined stroke within the pipette to draw liquid into and to
dispense liquid from the pipette,
the improvement comprising
a pipette calibration stop mounted adjacent said end of the barrel
and being adjustably positionable along the axis of travel of the
plunger, such that the pipette can be adjustably positioned with
respect to the stroke of said plunger, and
means holding the pipette against the pipette stop.
2. The precision liquid pipetting device of claim 1 wherein the
improvement further comprises a semi-resilient tip on the end of
the plunger flexibly embracing the interior walls of the
pipette.
3. The precision liquid pipetting device of claim 1 wherein the
means holding the pipette against the pipette stop is a cylindrical
sleeve integral with the stop upon which the pipette press
fits.
4. The precision liquid pipetting device of claim 1 wherein the
means holding the pipette against the pipette stop is a collet
threaded to said end of the barrel.
5. The precision liquid pipetting device of claim 4 wherein the
improvement further comprises
a semi-resilient truncated conical pipette retainer encompassing
the pipette;
a male threaded portion on said end of the barrel and a conical
seat within the threaded portion that diverges toward said end;
and
corresponding female threads on the collet for forcing the retainer
against the seat when threaded upon the male threads to retain the
pipette against the pipette stop.
6. In a precision liquid pipetting device having an elongated
barrel,
a pipette mounted upon one end of the barrel,
a plunger carried in part within the barrel and reciprocable with a
predetermined stroke within the pipette to draw liquid into and to
dispense liquid from the pipette, and
coil spring means within the barrel for normally biasing the
plunger to a withdrawn end of its stroke, the improvement
comprising
a pipette calibration stop mounted adjacent said end of the barrel
and being adjustably positionable along the axis of travel of the
plunger such that the pipette can be adjustably positioned with
respect to the stroke of said plunger;
means holding the pipette against the pipette stop; and
one of a series of end caps threaded to the barrel at its other
end, each of the series having a shoulder spaced a different
distance toward the spring means and by abuttment selectively
limiting the travel of the plunger at the withdrawn end of its
stroke.
7. The precision liquid pipetting device of claim 6 wherein the end
cap is generally circular with a diameter greater than the barrel
and has a flattened face for preventing roll of the pipetting
device when resting upon a generally horizontal surface.
Description
This invention relates generally to precision liquid pipetting
devices and more particularly to improved means for adjusting the
stroke of and calibrating the capacity of pipetting dispensers.
The principal object of this invention is to provide improved means
for calibrating the volumetric capacity of a pipetting dispenser
with a reproducible high degree of accuracy.
Another object of this invention is to provide simple means for
selectively varying the volume of liquid dispensable from a
micropipette of a particular size.
Other objects and advantages of the invention will become apparent
upon consideration of the following description and the
accompanying drawings wherein
FIG. 1 is an overall view of one embodiment of precision pipetting
device employing a disposable pipette of relatively large
diameter;
FIG. 2 illustrates another embodiment of precision pipetting device
which employs a disposable micropipette of much smaller
diameter;
FIG. 3 is a cross-sectional view of the barrel and plunger assembly
of the pipetting device of FIG. 1;
FIG. 4 is a cross-sectional view of the end of the barrel of the
pipetting device of FIG. 3 with a different end cap in place;
FIG. 5 is an end view of the cap ends of the pipetting devices of
FIGS. 1 and 2;
FIG. 6 is a cross-sectional view of the calibration assembly of the
pipetting device of FIG. 2;
FIG. 7 is a cross-sectional view of an alternate end construction
for the pipetting device of FIG. 2; and
FIG. 8 is a cross-sectional view of the calibration assembly of the
pipetting device of FIG. 1.
This invention relates to improvements in precision pipetting
devices of the type generally disclosed in M. E. Drummond, et al.
U.S. Pat. No. 3,606,086 issued Sept. 20, 1971 for Microdispensing
Device. The devices include an elongated barrel 1 and a disposable
pipette demountably mounted on the barrel, such as the extruded
plastic pipette 2 of relatively large diameter illustrated in FIG.
1 or the smaller precision glass micropipette 2' illustrated in
FIG. 2. A reciprocable plunger 3 carried in part within the barrel
1 has one end within the pipette to draw liquid into and to
dispense liquid from it.
Each of the described embodiments of barrel 1 has an interior bore
into which the plunger 3 passes and is affixed to a plunger holder
4 at one end. The coil spring means 5 within the bore of the barrel
seats at one end against the barrel itself and at the other end
against a seat 6 on the plunger holder, for example, to bias the
plunger toward the withdrawn end of its stroke as is shown in FIGS.
3 and 4.
A series of end caps 7, 7', or more, each thread into the open end
of the barrel and each has a bore through which the plunger holder
4 slides. These threaded end caps 7, 7' have a generally circular
head 8 substantially larger in diameter than the barrel 1 which is
flattened as at face 9 shown in FIG. 5 to prevent roll of the
pipetting device when it is resting upon a generally horizontal
surface. Each end cap 7, 7' also has a shoulder 10 spaced from its
threads toward the coil spring means against which the plunger
holder seat 6 abuts as the spring means biases the plunger toward
the withdrawn end of its stroke. As will be noted by a comparison
of FIGS. 3 and 4 the shoulder 10 of each of the series of end caps
7, 7', etc. is spaced a different difference from its threads so
that by interchanging end caps one can selectively adjust the
length of the plunger stroke in predetermined increments. A button
head 11 threads into the remote end of the plunger holder 4 for
manipulation of it. The thumb or forefinger of the user presses
upon this button head and simultaneously pulls against the head 8
of end cap 7, 7', etc. to extend the plunger 3. The bias coil
spring means 5 returns the plunger 3 to the withdrawn end of its
stroke.
The embodiment of dispenser illustrated in FIG. 1 includes a
plunger 3 which carries at its free end a semi-resilient tip 15
which is substantially larger in diameter than the plunger and
flexibly embraces the interior walls of the pipette 2 to force
fluid from or draw fluid into the pipette. A similar construction
is shown in FIG. 6 in the tip 15' of the other embodiment of the
invention. The precision micropipette 2' can be formed with an
internal diameter so precise that for small diameters the plunger 3
may be deployed within it without any tip 15' as is shown in FIG.
7.
The device of FIG. 1 has a pipette stop 20 mounted adjacent to the
pipette-end of the barrel which is adjustably positionable along
the axis of travel of the plunger. In that embodiment the pipette
stop 20 as shown in FIG. 8 comprises a hub 21 with an interior bore
22 which slides upon a cylindrical tip 23 of substantially the same
diameter which protrudes from that end of the barrel 1 and sleeves
over reciprocable plunger 3. Set screw 24 in the hub 21 of the
pipette stop 20 can be tightened against cylindrical tip 23 to
position the stop 20 at any point along it. A smaller diameter
sleeve portion 25 of the stop 20 carries the flanged end of plastic
pipette 2 press fit upon it with its flange 26 seated against hub
21. By precisely positioning stop 20 on the protruding cylindrical
tip 22 of the barrel, one can precisely calibrate the volume of the
pipette into which liquid is drawn and from which it is dispensed
for any particular plunger stroke.
The structure of the dispenser embodiment of FIG. 2 is similar in
function. As is illustrated more particularly in FIG. 6 the pipette
stop 20' has a bore for the plunger 3 which is smaller than the
diameter of the pipette 2' that mounts at the end of the barrel.
The stop 20', too, is adjustably positionable along the axis of
travel of the plunger 3 by shifting its position within a
cylindrical bore 28 in the end of barrel 1 and by holding it there
with set screw 24'. The pipette 2' seats against the pipette stop
20' and is held against it by a knurled collet 30 having female
threads which thread upon a male threaded portion 31 of the barrel
1. A conical seat 32 within the threaded portion 31 of the barrel
and next to bore 28 diverges outwardly toward the collet. A
semi-resilient truncated generally conical pipette retainer 33
encompasses the pipette and is forced against the conical seat 32
as the collet 30 threads upon the threaded portion 31 of the
barrel.
The precision pipetting dispensers of this invention provide the
user with a means for precisely measuring and dispensing volumes in
the order from 1 to 100 lambda or larger. They also provide a given
dispenser with a variety of stroke lengths by an appropriate series
of interchangeable end caps 7, 7', etc. Accuracy and
reproducibility in the order of .+-.1 percent is easily attained.
The dispenser is easily calibrated by placing in either of pipettes
2 or 2' a calibration tool which is nothing more than a wire or rod
of predetermined length, loosening the set screws 24, 24' and in
the embodiment of FIG. 2 loosening collet 30 also. With the
calibration tool of known length slipped into the open end of the
pipette, the pipette is placed vertically with that end against a
flat horizontal surface so that the end of the tool aligns
precisely with the end of the pipette. This automatically positions
the loosened pipette stop for correct calibration for that
particular calibration tool. The set screw 24, 24' is then
tightened in this position and, in the embodiment of FIG. 2, the
collet is tightened to hold the pipette against the stop 20'. The
press fit of pipette 2 upon sleeve portion 25 of the stop 20 in the
embodiment of FIG. 1 holds that pipette in place against hub 21 of
the stop.
The two embodiments of this invention have been described for
illustrative purposes only. The invention is defined in the
following claims.
* * * * *