U.S. patent number 3,913,564 [Application Number 05/463,601] was granted by the patent office on 1975-10-21 for anaerobic specimen collecting and transporting device.
Invention is credited to Richard C. Freshley.
United States Patent |
3,913,564 |
Freshley |
October 21, 1975 |
Anaerobic specimen collecting and transporting device
Abstract
An anaerobic specimen collecting and transporting device is
described incorporating a transporting tube having a suitable
transport media therein; a removable closure is provided for the
tube and incorporates a swab slidably mounted therein; the closure
is compartmentalized and includes a frangible ampule containing a
liquid for combination with a hydrogen generating substance to
remove oxygen from within the device that would otherwise be
destructive of the collected specimen.
Inventors: |
Freshley; Richard C. (Phoenix,
AZ) |
Family
ID: |
23840669 |
Appl.
No.: |
05/463,601 |
Filed: |
April 24, 1974 |
Current U.S.
Class: |
600/572; 604/1;
435/303.2; 435/309.1; 435/307.1; 435/304.2 |
Current CPC
Class: |
A61B
10/02 (20130101); A61B 10/0096 (20130101); G01N
2001/028 (20130101) |
Current International
Class: |
C12M
1/26 (20060101); A61B 10/00 (20060101); C12M
1/30 (20060101); G01N 1/02 (20060101); A61B
010/00 () |
Field of
Search: |
;128/2W,2B,2R,2F,269
;195/127 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Howell; Kyle L.
Attorney, Agent or Firm: Cahill, Sutton & Thomas
Claims
I claim:
1. An anaerobic specimen collecting and transporting device
comprising:
a transport tube for enclosing a specimen and maintaining the
specimen in a predetermined atmosphere;
a compartmentalized closure removably secured to, and sealingly
engaging, said tube;
a specimen gathering swab slidably mounted in said closure and
extending through said closure into said tube;
said closure including a first compartment containing a frangible
ampule having a liquid therein;
a second compartment in said compartmentalized closure
communicating with the said first compartment and containing a
hydrogen generating substance, said substance, when combined with
said liquid, producing hydrogen; and
a third compartment in said compartmentalized closure communicating
with said second compartment, containing a catalytic material for
producing a combinative reaction of hydrogen from said hydrogen
generating substance and oxygen in the atmosphere within said
device.
2. The combination as set forth in claim 1 wherein said liquid is
water and said hydrogen generating substance is responsive to
contact with water and generating hydrogen.
3. The combination as set forth in claim 1 wherein said liquid is
water and said hydrogen generating substance is magnesium.
4. The combination as set forth in claim 2 wherein said catalytic
material is palladium.
5. An anaerobic specimen collection and transporting device
comprising:
a transport tube for enclosing a specimen and maintaining the
specimen in a predetermined atmosphere;
a compartmentalized closure removably secured to, and sealingly
engaging, said tube;
a specimen gathering swab slidably mounted in said closure and
extending through said closure into said tube;
said closure including a first compartment containing a frangible
ampule having a liquid therein; and a second compartment
communicating with said first compartment containing a substance
chemically activated by said liquid to remove oxygen from the
atmosphere within said device; and
said tube including a viscous anaerobic transport media for
receiving an end of said swab when the latter is slidably lowered
into said tube.
6. The combination as set forth in claim 5 including a divider
removably mounted between said tube and closure and extending into
said tube for receiving said swab and maintaining separation of
said swab and said transport media until said device is to be
used.
7. The combination as set forth in claim 5 wherein said liquid
contains CuSO.sub.4 and H.sub.2 SO.sub.4 and wherein said
chemically activated substance is steel wool.
Description
The present invention pertains to devices for conveniently
collecting and preserving an anaerobic culture specimen for
subsequent transportation to a laboratory for analysis.
The collection of anaerobic culture specimens from wound drainage
and tissues requires careful handling and preservation to prevent
destruction of the specimens prior to analysis; since the specimens
are frequently collected in a physicians office or other location
remote from a laboratory having proper facilities for analysis of
the specimen; the handling of the specimens immediately after
collection and prior to analysis in the laboratory is of great
significance. Prior art devices for maintaining the proper
conditions for preservation of such anaerobic samples are usually
complicated and cumbersome. While portability of bacteriological
specimens is a recognized desideratum, there does not appear to be
any existing device capable of protecting the anaerobic specimens
immediately subsequent to collection and prior to analysis.
It is therefore an object of the present invention to provide a
device for the convenient collection and preservation of anaerobic
specimens.
It is another object of the present invention to provide a device
for anaerobic specimen collection and transportation that is
self-contained and upon actuation will provide a continuous
deoxifying effect to prevent destruction of the anaerobes.
It is still another object of the present invention to provide a
device that is small and readily portable while providing the ready
collection of anaerobic specimens combined with the subsequent
preservation of the specimens during transportation and storage
prior to analysis.
These and other objects of the present invention will become
apparent to those skilled in the art as the description thereof
proceeds.
The present invention may more readily be described by reference to
the accompanying drawings, in which:
FIG. 1 is an elevational view partly in section of apparatus
constructed in accordance with the teachings of the present
invention; and
FIG. 2 is an elevational view of a portion of the apparatus shown
in FIG. 1 modified to show another embodiment of the present
invention;
FIG. 3 is a cross-sectional view of FIG. 1 taken along lines
3--3;
FIG. 4 is a cross-sectional view of FIG. 1 taken along lines
4--4.
Referring now to FIGS. 1, 3 and 4 a disposable threaded transparent
plastic tube 10 is provided with a closure 12 threadedly engaged to
the open end thereof. The closure 12 is compartmentalized and
includes a first compartment 14 containing frangible ampules 16
which, in the embodiment chosen for illustration in FIG. 1,
contains water. The compartment 14 is defined by a vertically
movable top 18 and a platform 20 having openings 22 therein to
permit the liquid, or water, in the ampules 16 to descend
therethrough.
A second compartment 24 is defined by the cylindrical space between
the outer walls of the closure 12 and a vertically extending tube
26 supported therein by radially extending flange 28. It may be
noted that the closure 12 may be formed integrally with the
platform 20, the tubes 26 and the flange 28. The second compartment
24 is provided with a hydrogen generating compound 30 which may
conveniently be formed of compressed pelletized powder magnesium
and compounds to catalytically control the reaction rate such as
sodium chloride. The compartment 24 communicates with the
compartment 14 through the openings 22, and the hydrogen generated
by the mixture of the water in the ampules 16 and the compound 30
is free to circulate from the compartment 24 through the device
including a third compartment 32.
The compartment 32 forms a catalyst chamber and contains a material
providing catalytic action for the combination of hydrogen given
off by the compound 30 with the free oxygen contained in the
atmosphere within the tube 10. A variety of catalytic materials may
be used; however, palladium coated alumina pellets 34 are
commercially available to provide suitable catalytic action to
facilitate the reaction between the generated hydrogen and the free
oxygen.
A porous mat 36 supports the pellets 34 and prevents pellets or
other catalytic compounds from entering the tube 10 but is porous
enough to permit the flow of gases throughout the apparatus. The
mat 36 may be made from any non-reactive material such as certain
metals but is preferably formed of asbestos. A swab 40 is slidably
mounted in the closure 12 and may be raised or lowered from the
position shown in FIG. 1. The swab may be lubricated at those
portions in contact with the closure as it passes through the
closure 12 by any sterile viscous non-water soluble lubricant.
The tube 10 is provided with a viscous semi-gelatinous transport
media of the type commercially available (for example, Stuart's
Formula Transport Media sold by Baltimore Biological Laboratories).
The transport media 42 is specifically modified to provide an
oxygen free environment for the preservation of anaerobic
specimens. The media may include a resazurin indicator that will
provide a pinkish hue to indicate the presence of oxygen and insure
that the media will not be used if it has deteriorated. The
transport media 42 may also contain cysteine as a reducing agent
for the reduction of any oxygen that may have diffused into the
media. Prior to the utilization of the apparatus described herein,
the swab 40 is maintained out of contact with the media 42; to
insure separation, a divider 44 is removably positioned between the
tube 10 and the closure 12 and may be conically shaped as shown in
FIG. 1.
Referring now to FIG. 2, an alternative form of the present
invention is shown wherein an enclosure 46 is provided with a
compartment 48 having liquid containing ampules 50 therein. The
compartment 48 communicates through openings 22 to a second
compartment 54 containing steel wool 56. A porous mat 58 separates
the compartments 48 and 54 from the tube 60. In the embodiment
shown in FIG. 2, the liquid contained within the ampules 50 is a
solution which will react with the steel wool to provide an oxygen
utilizing reaction to remove the free oxygen in the atmosphere
within the apparatus. The liquid, for example, may be formed of
Tween 80 (commercially available from Baltimore Biological
Laboratories) and may contain CuSO.sub.4, and H.sub.2 SO.sub.4.
The operation of the apparatus of the present invention may be
described as follows. The device is stored in substantially the
form shown in FIG. 1. When its use is required, the closure 12 is
unscrewed from the tube 10 and the divider 44 is discarded. The
swab 40 is then utilized in the well known manner for collecting an
anaerobic culture specimen from a wound or the like. The swab
remains, during this period of time, as a unit with the closure 12.
Subsequent to the collection of the specimen, the closure 12 is
returned to the tube 10 and the two are threadedly engaged.
The swab 40 is then lowered into the transport media 42 by sliding
it downwardly. The ampules 16 are then crushed by applying pressure
to the top 18; the water drops through the openings 22 into the
compartment 32 in contact with the hydrogen generating pellets 30.
A hydrogen rich atmosphere is thus generated and circulates through
the device. The hydrogen combines with oxygen present in the
atmosphere within the device in the presence of the catalyst 34.
The hydrogen rich atmosphere combined with the catalyst provide
continuous oxygen purging during storage and transportation of the
device to a laboratory having facilities for anaerobiasis. When the
storage and transportation time is very brief, it may not be
necessary to utilize the transport media and rely instead on the
hydrogen rich atmosphere to combine with the oxygen present to
produce a non-destructive environment for the anaerobic
specimen.
* * * * *