U.S. patent number 3,903,314 [Application Number 05/460,565] was granted by the patent office on 1975-09-02 for process for texturizing microbial broken cell material having reduced nucleic acid content by a deep oil frying technique.
This patent grant is currently assigned to The Standard Oil Company. Invention is credited to Kwei C. Chao.
United States Patent |
3,903,314 |
Chao |
September 2, 1975 |
**Please see images for:
( Certificate of Correction ) ** |
Process for texturizing microbial broken cell material having
reduced nucleic acid content by a deep oil frying technique
Abstract
Protein containing broken microbial cell material is treated for
nucleic acid removal and then texturized by homogenizing an aqueous
slurry of ruptured cells and further conditioning the ruptured
cells by a combination of heating, drying, and rehydration which
may include pH adjustment, calcium treatment, and other formulation
ingredients.
Inventors: |
Chao; Kwei C. (Naperville,
IL) |
Assignee: |
The Standard Oil Company
(Chicago, IL)
|
Family
ID: |
23829219 |
Appl.
No.: |
05/460,565 |
Filed: |
April 12, 1974 |
Current U.S.
Class: |
426/656; 426/506;
530/821; 426/441; 530/371; 530/824; 530/825 |
Current CPC
Class: |
A23J
1/008 (20130101); C12N 1/08 (20130101); A23J
1/18 (20130101); A23J 3/22 (20130101); A23J
3/20 (20130101); Y10S 530/825 (20130101); Y10S
530/824 (20130101); Y10S 530/821 (20130101) |
Current International
Class: |
A23J
1/18 (20060101); A23J 3/22 (20060101); A23J
3/00 (20060101); A23J 3/20 (20060101); A23J
1/00 (20060101); C12N 1/08 (20060101); A23j
003/00 () |
Field of
Search: |
;426/62,148,204,364,369,428,212,441 ;95/1,2,28R,104 ;260/112R |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Monacell; A. Louis
Assistant Examiner: Yoncoskie; R. A.
Attorney, Agent or Firm: Bellamy; Werten F. W. Gilkes;
Arthur G. McClain; William T.
Claims
I claim:
1. A process for developing texture in broken microbial cell
material, comprising the steps of:
a. preparing an aqueous slurry of said microbial cell material,
containing from about 5 to about 15 wt. % cells;
b. disintegrating the cells in the slurry by passing them through a
homogenizer;
c. removing nucleic acid from the homogenized cell slurry by
adjusting the pH to between 7.5 and 8.0, and then heating the cell
slurry at a temperature of about 75.degree. to 95.degree.C. for
about 5 to 30 minutes;
d. adjusting the pH of the homogenized cell slurry, having reduced
nucleic acid, from step (c) with calcium hydroxide to between 10
and 12, heating the said slurry up to about 75.degree. to
95.degree.C. and then cooling immediately, neutralizing with
hydrochloric acid to a pH of 7.5 and removing the excessive calcium
ion in the form of calcium chloride;
e. recovering the homogenized cell slurry from step (d) and adding
a portion thereof to an edible oil having a temperature from about
120.degree. to 160.degree.C;
f. stirring the homogenized cell slurry as defined in step (e) and
the edible oil mixture thereby breaking up the soft mass of cell
material into smaller lumps and frying to effect the continuous
removal of water as vapor; and
g. removing the excess oil by passing the fried cell material
through a strainer to produce a product which is crisp, crunchy,
chewy, has a bland or pleasing taste and resists dispersion in
water.
2. A process of claim 1 wherein the product from step (g) is
rehydrated by boiling it in water and recovering the product which
is elastic and has a spongy structure.
3. A process of claim 1 wherein the rehydration of the product from
step (g) is carried out in the presence of a formulated juice
consisting essentially of artificial flavor, meat seasonings and
enhancers, sugar, salt and pepper.
4. A process of claim 3 wherein the formulated juice consists
essentially of meat seasonings and enhancers, sugar, salt and
pepper.
5. A process of claim 3, wherein the rehydrated product is dried in
a hot air oven at 70.degree. - 80.degree.C. to produce a product
which is crisp, crunchy, chewy and tastes like beef.
6. A process of claim 4 wherein the rehydrated product is dried in
a hot air oven at 70.degree. - 80.degree.C. to produce a product
which is crisp, crunchy, chewy and tastes like liver.
7. A process of claim 1 wherein the broken microbial cells are
yeast cells.
8. A process of claim 7 wherein the yeast is selected from the
group consisting of Saccharomyces cerevisiae, Saccharomyces
carisbergensis, Saccharomyces fragilis and Candida utilis.
9. A process of claim 8 wherein the yeast is Candida utilis.
10. A process of claim 1 wherein the broken microbial cells are
bacteria cells.
11. The process of claim 1 wherein the broken microbial cells are
fungi cells.
Description
BACKGROUND OF THE INVENTION
In recent years much attention has been directed toward the
development of new sources of protein for human consumption. There
exists a need for protein material which can be incorporated in
foods or usable as a basic proteinaceous substance for human
consumption. Rapid increases in world population have made the
continued dependence on traditional sources of protein highly
impractical. Moreover, the supply of protein from typical sources
of protein, such as animal meat and certain vegetables, is
inadequate to provide balanced diets sufficient to satisfy needs of
humans throughout the world. These factors coupled with the
difficulties associated with providing protein from traditional
sources because of drought, flooding and both animal and crop
diseases gives critical significance to this situation.
One possible solution to the problem of supplying the ever
increasing need for food protein is provided by processes for the
bio-synthetic manufacture of protein through the growth of
microorganisms of hydrocarbon or other substrates. It is known, for
example, that microorganisms such as bacteria and yeast, which are
grown by single-cell reproduction, contain high proportions of
proteins and can be utilized directly in foods as whole-cell
material or can be treated to recover protein isolate. Recent
efforts have shown that microorganisms, grown on hydrocarbon
substrates can be successfully used in animal feeds; but as yet
these microorganisms have not been commercially accepted in food
preparations suitable for human consumption.
With the development of successful processes for the synthetic
production of protein-containing microorganisms (sometimes referred
to herein as single cell proteins), an urgent need has developed
for methods of texturizing such single-cell protein materials in a
manner sufficient to render them suitable for use in food products.
Generally, single-cell protein is initially produced as a wet paste
and then is subsequently converted into dry powder form. This dry
powder, similar in appearance and feel to flour, lacks the texture
and food-like sensation to the mouth necessary to make an
attractive food. Moreover, when placed in water, the powdered
single-cell protein rapidly reverts back to single-cell form.
Ideally, therefore, it is desirable to impart properties such as
chewiness, crispness, resistance to dispersion in water and the
like to such single-cell proteins in order that they may be used to
full advantage as additives to and substitutes for natural
foods.
Various techniques are known in the art for effecting texture
formation in soy bean based protein, such techniques are not
generally applicable to single-cell technology and are ineffective
in such application.
The use of "texturized vegetable proteins" (hereinafter referred to
as TVP) in food products, especially as meat extenders or analogs
has been increasing rapidly. Many people predict that the market
for TVP may reach 10% of all domestic meat consumption by the year
1985. The technology of texturizing soy protein is well
established. Presently, there are mainly two types of TVP produced
on the market. Namely, the expanded vegetable protein is made by a
thermoplastic extrusion technique and the spun vegetable protein by
a fiber spinning technique. TVP is characterized as having
structural integrity and identifiable texture. These features
enable it to withstand hydration in cooking and other procedures
used in preparing the food.
In order for single-cell proteins (SCP) to compete with vegetable
seed proteins and to share the protein market in the future, it has
to be texturized and processed for nucleic acid removal.
The human metabolic system produces uric acid as in the metabolism
of ribonucleic acid (RNA). Since man does not have a uricase enzyme
system, uric acid is not further broken down and is excreted with
urine. Because uric acid has a very low solubility in water it will
accumulate in the body in crystalline form if produced in larger
quantities than the body can excrete. This may lead to the
condition known as gout. It is, therefore, recommended by many
nutritionists that the RNA intake in diet be kept at a low
level.
Microbial cells, or single-cell protein (SCP) materials, contain
from 4 to 30 percent or more nucleic acids according to their
growth rates and the phase of growth. Usually, the higher nucleic
acid contents of the microbial cells are associated with rapid
growth phases. If the microbial cells are to be used as a protein
source in human feeding, nutritionists recommend generally that the
amount of nucleic acids contributed by SCP to diet should not
exceed 2 grams per day.
The calculated RNA contents of some conventional protein sources
are given in Table I. These vary from 0 to 4 percent. The RNA
content of SCP generally ranges from 8 to 18 percent for
exponential growth phase cells. In SCP intended for human
consumption the RNA content should preferably be reduced to about 2
percent on cell dry weight basis.
TABLE I ______________________________________ RNA Content
(Calculated) of Various Protein Sources
______________________________________ FOOD % RNA
______________________________________ Milk 0 Beans 1.7 Salmon 2.4
Chicken 2.9 Beef 3.7 Pork 4.1 Liver 9.3 Anchovies 14.5 SCP 8 to 18
______________________________________
A preferred way of utilizing SCP material is in the form of broken
cells. In this form, there is a need for the development of means
for removing nucleic acids from the microbial cell material. This
is desirably accomplished with a minimum loss of protein materials
from the cells in order to maintain the nutritional attractiveness
of such SCP materials.
An approach to accomplishing the above goals is to take advantage
of the enzyme system already present within the unicellular
microorganisms, activating the latent enzymes so that they act
degradatively or hydrolytically in a selective manner upon the
particular nucleic acid species present in the SCP material. One
such process has been described in U.S. patent application Ser. No.
838,453, filed July 2, 1969, now abandoned, wherein magnesium ion
is withheld from the nutrient system during fermentation to enhance
the activity of RNase and simultaneously deactivate RNA polymerase.
Preferred conditions include heating the microbial culture to
45.degree. to 100.degree.C. under alkaline conditions, cooling, and
then adding glucose as a leakage promoter in a final fermentation
stage.
Another process has been described by Ohta, Maul, Sinskey and
Tannenbaum in a paper presented at the 160th ACS National Meeting,
Chicago, Ill., September, 1970, where a very dilute (less than 1
wt.% cells) aqueous slurry of yeast cells is heated in a specific
temperature cycle: very briefly (3-17 seconds) at
65.degree.-70.degree.C. to shock the cells; then 1-2 hours at
45.degree.-50.degree.C.; and finally about 1 hour at
55.degree.-60.degree.C. The heat-shock step is claimed to be
critical. The optimum pH is from 5.0 to 6.5, in contrast to the
alkaline conditons preferred in the first process above.
Both of the described process reduce the nucleic acid content of
cellular materials but are limited to operation on the relatively
dilute fermentation broths.
Relative to drying yeast material by deep oil frying, a procedure
is described in the completed Holland specification No. 156,387,
filed May 5, 1969. Briefly, this process relates to frying a yeast
whole cell paste to obtain a product with meaty flavor. However,
this document does not mention (1) the use of deep oil frying
technique for texturization of yeast broken cells, (2) the
importance of the removal of nucleic acid before the material is
fried and (3) the rehydration step subsequent to frying the cell
material.
SUMMARY OF THE INVENTION
One object of this invention is to provide a novel and improved
process for texturizing broken cell microbial material which has
been treated for the removal of nucleic acid to a level generally
acceptable in food products intended for human consumption.
Another object is to provide new and useful food products and food
ingredients comprising texturized SCP materials having a suitably
low nucleic acid content.
The objects are accomplished by a process of adjusting the pH of an
homogenized slurry of broken microbial cells to between 7.5 and 8.0
and then heating the cell slurry at a temperature of about
75.degree. to 95.degree.C. for about 5 to 30 minutes. The cell
paste, with or without further calcium treatment, is added to an
edible oil and stirred slowly as it is deep fried to texturized
form. This texturized broken cell material may be rehydrated,
modified by flavor additives or other formulation ingredients and
redried.
The resulting improved SCP food component has lost substantially
none of its protein content and contains a reduced amount of
nucleic acids. This product can be used as a meat extender or snack
food.
DESCRIPTION OF THE INVENTION
This invention relates to a novel method for texturizing broken
cell microbial material which has been treated for the removal of
nucleic acid together with the novel and improved food products
obtained thereby.
It has been found that most of the nucleic acid content of
single-cell microorganisms can be removed by adjusting the pH of an
homogenized slurry of broken microbial cells under controlled
temperature conditions. This is accomplished with essentially no
attack on the protein material. The microbial cell material is
added to an edible oil and stirred slowly as it is deep fried to
texturized form. The resulting texturized product is crisp,
crunchy, chewy, has a bland or pleasing taste and resists
dispersion in water. This texturized product may be used as a
snack, or rehydrated and used as an extender. Additionally, the
dried broken cell material may be rehydrated by cooking with
various ingredients based on formulation and then redried to be
used as a snack or meat extender.
The practice of this invention is broadly applicable to
microorganisms and particularly to those organisms classified as
bacteria, yeasts, and fungi. By way of illustration bacteria such
as those listed in Table II, yeasts such as those listed in Table
III and fungi such as those listed in Table IV are suitable
microorganisms.
TABLE II -- Suitable Bacteria
Acetobacter sp.
Arthrobacter sp.
Bacillus subtilis
Corynebacteria sp.
Micrococcus sp.
Pseudomonas sp.
TABLE III -- Suitable Yeasts
Candida curvata
Candida lipolytica
Candida pulcherima
Candida utilis
Hansenula anomala
Hansenula miso
Oidium lactis
Saccharomyces carlsbergensis
Saccharomyces fragilis
Trichosporon cutaneum
Saccharomyces cerevisiae
Candida parapsilosis
Hansenula wickerhamii
Pichia pastoris
Pichia haplophyla
TABLE IV - Suitable Fungi ______________________________________
Aspergillus niger Penicillium notatum Aspergillus glaucus
Penicillium chrysogenum Aspergillus oryzae Penicillium glaucum
Aspergillus terreus Penicillium griseofulvum Aspergillus itaconicus
______________________________________
Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis,
and Saccharomyces carlsbergensis are preferred starting materials
for the process of this invention, however, because each has been
generally regarded by the F.D.A. as safe for use in food
products.
Microbial cells suitable for the process of this invention may be
grown aerobically in either a batch or continuous manner. Any
suitable carbon-affording substrate may be employed although, for
purposes of preparing SCP products for use in foods, an ethanol
substrate is preferred. Any conventional combination of mineral
nutrient elements may be employed. A convenient source of nitrogen
is ammonia which may also be supplied to the fermentor as required
to maintain the pH of the fermentation broth, preferably within the
range from 3.5 to 5.5. Cells which have been grown at a rapid rate
usually have a higher nucleic acid content while those grown more
slowly tend to have a more permeable cell wall. Either of these
types, as well as cells grown under oxygen-limiting or
substrate-limiting conditions may be usefully treated according to
the present invention to afford improved and acceptable foods and
food components suitable for human consumption.
Rupture of the microbial cells may be accomplished by any suitable
physical means at appropriate temperatures. Thus, for example, any
homogenizer, colloid mill, ball mill or ultrasonic device may be
employed.
Using oil as a heat transfer medium, frying is employed as the
method for drying the broken microbial cell material. This drying
process permits the escape of water vapor from the mass thereby
causing the formation of porous structure of the fried material
which gives a spongy texture when hydrated. The escaping water
vapor carries some of the objectionable yeasty flavors and the
frying imparts a meat like flavor.
The oil or fat used in the process of this invention may be any
edible oil or fat, from animal or vegetable source, e.g., corn oil,
peanut oil, lard, soya bean oil, palm oil or hydrogenated or
partially hydrogenated palm oil, or partially hydrogenated,
deodorized fish oil. Any edible oil or fat can be used with or
without the addition of an antioxidant. However, different flavors
and different problems of operation can be attributed to the kind
of oil employed. It appears that the animal fats may have the
advantage of giving genuine meaty flavor.
When the frying is conducted at lower temperatures, more oil is
absorbed by the product. However, any excessive oil or fat can be
removed by centrifugation or by a rehydration treatment.
The yeast material to be fried is usually bland in flavor or has a
slight yeasty after-taste. This flavor can be improved greatly by
adding flavoring material like artificial flavor,
monosodium-glutamate, salt, pepper, sugar, etc. The light color can
be modified with coloring agents and nutrients like vitamins,
minerals, and amino acids, particularly methionine.
Various gelling or stiffening agents can be added to give proper
consistency, elasticity and viscosity. This facilitates subsequent
processing and texturization of the product. The calcium treatment
serves such a purpose to give more flexibility in choosing the
desired quality of texture and chewy eating character.
As a meat extender, the texturized product is to be used in wet
form either rehydrated by the user immediately before being mixed
with meat, or in the ready-to-use form which may be canned or in
the frozen state. The rehydration is carried out by soaking or
boiling the fried product in water and removing excess oil by
decantation. Alternatively, the fried texturized product can be
rehydrated in a precooking process wherein additives are formulated
and added. The precooked texturized product can be used as a meat
extender in a substance to be cooked or as a ready-to-eat cooked
meat substitute. Moreover, it can be in moist form or fried again
to give snack type food.
It is contemplated that the development of various specific food
products is possible with modifications of the basic technique of
deep oil frying which can be followed by hydration and precooking.
The basic scheme of our inventive process to texturize the
microbial cell material is depicted as follows: ##SPC1##
SPECIFIC EMBODIMENTS OF THE INVENTION
The following examples are illustrative, without limitation, of my
invention.
EXAMPLE I
Yeast cells are disintegrated by passing through a Manton-Goulin
homogenizer. The nucleic acid is removed by adjusting the pH to 7.5
to 8.0 and then heating to a temperature of 75.degree. to
95.degree.C. for 5 to 30 minutes. The heat-coagulated material is
treated with calcium hydroxide thereby raising the pH to between 10
and 12 and heated to between 75.degree. to 95.degree.C., then
cooled down right away. The mixture is neutralized back to pH 7.5
by adding 1 to 6 normal HCl and removing the excessive calcium ion
in the form of calcium chloride. The recovered paste material has
73 percent moisture. A portion of the material is dropped into
Wesson oil being heated at 160.degree.C. The temperature drops to
110.degree.C. right after the charge. The material submerged in the
hot oil is carefully and slowly stirred to break up the soft mass
into various smaller lumps. As the process is going on, the water
is continuously removed as vapor, and the temperature is gradually
rising, with the accompanyment of changes of dimension, density,
texture and color. The material is completely dried as the
temperature comes back to about 160.degree.C. with browned color
and crispy texture. The fried material is passed through a strainer
to remove the excessive oil. The product is crispy and tastes like
ground meat. No yeasty flavor or odor could be detected. It has a
porous structure and when hydrated, by boiling in water, it swelled
and became elastic with a spongy structure. Hamburger test
indicates that the flavor and texture are acceptable when 15 grams
of the moist texturized material (corresponding to 7.5 grams of dry
matter) are mixed and cooked with 100 grams of fresh hamburger
meat.
EXAMPLE II
The material is prepared as described in EXAMPLE I and then charged
to the hot oil at 150.degree.C. in the form of strands by injecting
through a syringe. Because of the low ratio of the material to the
oil, no drastic change of temperature is observed. The material in
the form of strands is quickly fired within 1 minute. The readiness
of the process can be judged by the change of density. The fried
material sinks to the bottom of the oil bath. The material still
keeps its strand form, but has irregular surface and a porous
structure made up of many individual hollow cell pockets. The
strands can be entangled during frying to give the appearance of
mesh work.
EXAMPLE III
The yeast material is prepared as described in EXAMPLE I, except
that no calcium treatment is carried out. The paste has a higher
moisture content of 83 percent. After it is fried and rehydrated,
the material has softer texture which appears to be more compatible
with hamburger meat than the one prepared in EXAMPLE I. A
difference in flavor cannot be noticed. The material is less
chewable than the one treated with calcium.
EXAMPLE IV
The fried material as obtained in EXAMPLE I is rehydrated by
cooking in a formulated juice composed of artificial beef flavor,
monosodium glutamate, hydrolyzed vegetable protein, sugar, salt and
pepper. The material is cooked until the juice is concentrated to
almost dryness. The moist cooked material tasted like cooked ground
beef. The wet cooked material is further dried in a hot air oven at
70.degree. - 80.degree.C. until it is dried and crispy. The dried
preparation tasted like fried meat bits.
EXAMPLE V
The procedure of EXAMPLE IV, except that no artificial beef flavor
was added. The cooked material tasted like liver.
* * * * *