U.S. patent number 3,902,852 [Application Number 05/378,934] was granted by the patent office on 1975-09-02 for apparatus for multiple sample application.
This patent grant is currently assigned to Universite de Sherbrooke. Invention is credited to Bernard Lemieux, Apostolos Sahapoglu, Dennis Shapcott.
United States Patent |
3,902,852 |
Lemieux , et al. |
September 2, 1975 |
Apparatus for multiple sample application
Abstract
A plurality of laterally spaced apart syringes are carried on a
rotatable rod, their plungers being fixedly secured in a drive bar
so that the plungers are simultaneously displaceable in one
position to aspirate a predetermined quantity of sample and in
another position to deliver a given quantity of the sample. Drive
means are provided for simultaneously displacing the drive bar in
the sample delivery direction and horizontally displacing the
sample delivery station.
Inventors: |
Lemieux; Bernard (Sherbrooke,
CA), Shapcott; Dennis (Lennoxville, CA),
Sahapoglu; Apostolos (Brossard, CA) |
Assignee: |
Universite de Sherbrooke
(Sherbrooke, CA)
|
Family
ID: |
4093956 |
Appl.
No.: |
05/378,934 |
Filed: |
July 13, 1973 |
Foreign Application Priority Data
Current U.S.
Class: |
73/863.32;
73/864.16; 141/130; 422/70 |
Current CPC
Class: |
G01N
35/1095 (20130101) |
Current International
Class: |
G01N
1/00 (20060101); G01n 001/14 () |
Field of
Search: |
;23/23R,253R,259,292
;73/425.6,425.4R ;141/130 |
References Cited
[Referenced By]
U.S. Patent Documents
|
|
|
3607097 |
September 1971 |
Auphan et al. |
3756920 |
September 1973 |
Kelbaugh et al. |
3776184 |
December 1973 |
Harrison |
|
Primary Examiner: Serwin; R. E.
Attorney, Agent or Firm: Cushman, Darby & Cushman
Claims
The embodiments of the invention in which an exclusive property or
privilege is claimed are defined as follows:
1. An apparatus for the simultaneous collecting of multiple samples
and for the simultaneous dispensing thereof onto a sample
separation means comprising, in combination:
a. a horizontally mounted rotatable support means;
b. a plurality of laterally spaced apart syringes carried on said
support means, each said syringe including a plunger and a
needle;
c. a horizontally mounted displaceable drive bar means fixedly
receiving each said plunger for simultaneously displacing said
plungers relative to said support means;
d. first station means disposed adjacent one side of said support
means and including a plurality of laterally spaced apart sample
receiving means, each said receiving means being aligned with an
associated needle;
e. second station means disposed adjacent the opposite side of said
support means for receiving the sample separation means; said
support means being rotatable from said first station means, where
said drive bar means is displaced in one direction to
simultaneously aspirate in each said syringe a predetermined
quantity of said samples, to said second station means where said
drive bar means is displaced in the opposite direction to
simultaneously deliver a given quantity of said samples on the
sample separation means.
2. An apparatus as defined in claim 1 further comprising drive
means for simultaneously displacing said drive bar means in the
sample delivery direction and horizontally displacing said second
station means.
3. An apparatus as defined in claim 2 further comprising guide
means for guiding the displacement of said drive bar means.
4. An apparatus as defined in claim 1 wherein said first station
means is tiltable to ensure immersion of the extremities of the
needles in said samples.
5. An apparatus as defined in claim 2 further comprising pinion
means operatively connected to said drive means and a toothed rack
fixedly mounted on said drive bar means whereby when said rack and
said pinion are in gear engagement, said drive bar means is
automatically displaced by said drive means.
6. An apparatus as defined in claim 1 wherein said drive bar means
includes lug means at each extremity thereof; said apparatus
further comprising vertically upstanding side walls each provided
with grooves for receiving said lug means of said drive bar means,
said grooves defining an endless track for said lug means.
7. An apparatus as defined in claim 1 further comprising means for
drying the sample when delivered on the sample separation
means.
8. An apparatus as defined in claim 1 wherein said support means
includes handle means for rotating said support means, said handle
means including weight means for providing vibration-free delivery
of said sample on the sample separation means.
9. An apparatus as defined in claim 6 wherein said side walls
include an additional groove for receiving said lug means to
thereby completely discharge the sample from each said syringe.
Description
This invention relates generally to laboratory apparatus and more
particularly to a sampling and discharging apparatus capable of
withdrawing into a plurality of syringes predetermined quantities
of liquid and of delivering same on a liquid separating paper or
plate for examination purposes.
In the laboratory, it is often necessary to perform analytical
tests on a large number of samples. It is therefore desirable that
such tests be performed within a minimum amount of time and with
extreme accuracy and precision to eliminate error. For example, on
dimensional chromatography of amino acids in biological sample has
become established as a standard technique for the screening of
metabolic disorders of the newborn. Thin layer chromatography
(often referred to as TLC) has been used which offers the
advantages of reduced development time, a homogeneous medium giving
improved separation, and the possibility of varying the separating
medium. Until now, the application of samples has usually been
performed manually and has required a great deal of time. Some
tests involve the examination of hundreds of thousands of samples
annually for abnormalities of amino acid excretion, organic acid
excretion, keto acid excretion and other metabolites. In recent
years, there has been developed a tremendous need for rapid
screening of body fluids for drug abuse. Because of the rapidity
and superiority of separation, thin layer chromatography has been
used largely for these purposes. Because of the relatively small
fluid capacity of the TLC plates, the application of samples
remains a time-consuming procedure. Several spotting devices have
been designed to deliver predetermined quantities of samples to
plates, but these are designed to apply aliquots of the same sample
in a highly reproducible fashion and not to deliver simultaneously
a large number of different samples.
It is therefore an object of this invention to provide an apparatus
which delivers samples in an automated manner rapidly and
reproducibly.
Another object of the present invention resides in the provision of
a compact laboratory apparatus for multiple sample application
capable of simultaneously withdrawing from a series of sample
containers a predetermined reproducible quantity of the sample and
of dispensing simultaneously the same onto a separation plate or
paper.
A further object of the present invention resides in the provision
of a novel semi-automatic apparatus for multiple sample application
to thin layer chromatography plates.
The present invention relates to an apparatus for multiple sample
application to a sample separation means which comprises a
horizontally-mounted rotatable rod means, a plurality of
laterally-spaced apart syringes carried on the rod means, each
syringe including a plunger and a needle, a horizontally-mounted
displaceable drive bar means fixedly receiving each plunger whereby
the plungers are simultaneously displaceable relative to the rod
means, first station means disposed adjacent one side of the rod
means and including a plurality of laterally-spaced apart samples
receiving means, each receiving means being aligned with an
associated needle, second station means disposed adjacent the
opposite side of said rod means for receiving a sample separation
means; the rod means is rotatable from the first station means,
where the drive bar means is displaced in one direction to aspirate
in each syringe a predetermined quantity of the sample, to the
second station means where the drive bar means is displaced in the
opposite direction to deliver a given quantity of the sample on the
sample separation means.
In one preferred form of the invention, the apparatus includes
drive means for simultaneously displacing the drive bar means in
the sample delivery direction and for horizontally displacing the
sample separation means to provide a uniform sample application
line thereon.
Having thus generally described the nature of the present
invention, particular reference will now be made to the
accompanying drawings showing by way of illustration the preferred
embodiment thereof, and in which:
FIG. 1 is a side elevational view of the apparatus made in
accordance of the present invention;
FIG. 2 is a top plan view of the apparatus shown in FIG. 1;
FIG. 3 is a cross-sectional view taken along lines 3--3 of FIG. 2
and showing one syringe needle in one associated sample;
FIG. 3a is a cross-sectional view taken along lines 3--3 of FIG. 2
and showing the syringe being filled with the sample;
FIG. 4 is a cross-sectional view illustrating the syringe carrying
rod being rotated in a counter-clockwise manner;
FIG. 5 is a cross-sectional view showing the syringe needle over
the sample separation means;
FIG. 6 is a broken-apart view similar to FIG. 5 but illustrating
the sample being discharged on the separation means;
FIG. 7 shows two sample application lines of two adjacent
syringes;
FIG. 8 is a cross-sectional view taken along lines 8--8 of FIG. 5;
and
FIG. 9 is a schematic representation of the travel of the drive bar
means.
Referring to FIGS. 1 and 2, the novel pick-up and dispensing
apparatus 20 comprises a base plate 22 and two vertically
upstanding side walls 24 and 26 fixed to the base plate 22 and
supporting therebetween for rotation a horizontally extending
circular rod 28 carrying a plurality of syringes 30 equidistantly
placed along the rod at right angles to the longitudinal axis
thereof. The syringes are retained within the rod by bolts 32 (see
FIGS. 3-5) and may be adjusted and removed by releasing the bolts.
Each syringe includes a needle 34 and a plunger 36 with its head
38. Rod 28 is supported in a pair of U-shaped members 40 screwed or
otherwise secured to side walls 24 and 26 but is free to rotate
therein. The head 38 of each plunger is held within a drive bar 42,
the ends of which are equipped with lugs 44 (one of which is shown
in FIG. 8). The lugs are received in grooves 46 provided in each
plate 40 and defining an endless track in which the lugs may
travel. Each groove includes an outer portion 46o, an inner portion
46i, and two end portions 46e.
Also secured on each plate 40, is a pinion 48 which is adapted to
engage a toothed rack 50 mounted on the drive bar 42, the function
of which will hereinafter be described. Rack 50 is mounted parallel
to the syringes 30.
Mounted at the front portion of the apparatus 20 and disposed
longitudinally along one side of the rod 28 is a first station
means which includes a rack 52 having a series of wells 54 to
receive therein a series of containers 56 containing the liquid
sample to be tested. Rack 52 is removably supported in openings 53
provided in side walls 24 and 26 and is also adjustable to receive
therebeneath a tapered member 58 which inclines the containers in a
proper aspirating position for the needles 34. Mounted at the rear
portion of the apparatus 20 and disposed longitudinally along the
opposite side of rod 28 is a second station means which includes a
sample separation means 60, which is preferably a thin layer
chromatography plate, supported on a longitudinally moveable base
62. The constituents of the thin layer chromatography plate are
well known to those skilled in the art and include silica gel and
cellulose.
A motor 64 is provided adjacent side wall 26 to drive appropriate
means, such as shafts 65 and gears 66 represented in dotted lines
in FIG. 2, which cooperate to actuate pinions 48 and the mechanism
for advancing the base 62 with its plate 60. An example of such
advancing mechanism may include a threaded bar 67 carring a
spring-release catch 68 for the base so that once the base has
moved a predetermined distance it may be disengaged and returned
manually to its starting position.
Drive bar 42 is manually displaceable relative to rod 28 and this
movement is assisted by means of two guide means 70 and 72 which
slide into two openings excentrically located in rod 28, one of
which is shown as 74 in FIG. 8. These guide means are fixedly
secured to the drive bar means 42 by appropriate securing means
76.
The description of the operation of the apparatus will now be
described.
Referring to FIG. 3, the apparatus is shown in its starting
position. All needles 34 have their extremities immersed in the
samples to be analyzed. As explained above, the angled member 58
pivots rack 52 to ensure that the extremities of the needles are
well immersed in the containers 56. In this position, the lugs 44
are located at 44a (see FIG. 9) in groove 46i and the syringe
plunger retaining drive bar 42 is in its closest position to rod
28. Drive bar 42 is then manually withdrawn thereby aspirating the
samples into all the needles and partly into the barrel of the
syringes. Lugs 44 are now in a position 44b. Then, by means of a
handle 78, rod 28 is rotated counter-clockwise (see FIG. 4) causing
lugs 44 to follow groove 46o until they reach the position 44c.
There, racks 50, which are integral with the drive bar 42 engages
pinions 48. Motor 64 is then started and the syringe plunger
retaining drive bar 42 is driven toward rod 28 thereby displacing
the fluid from the syringes while the chromatography plate is moved
laterally simultaneously (see FIGS. 5, 6 and 7). For a
vibration-free delivery, handle 78 is provided with a weighted
extension 80; other means of securing the rod 28 during delivery
will be evident to those skilled in the art. Lugs 44 are in
position 44d. The quantity of sample being delivered is determined
by the toothed portion 82 of rack 50 which includes a straight
non-toothed portion 84 (see FIG. 6) where pinion 48 is no longer
engaged with rack 50 and rotates freely until the motor is stopped.
After delivery is completed, the drive bar 42 is manually returned
to its starting position with lugs 44 in grooves 46i. The process
is then repeated for other samples on other chromatography
plates.
Although not illustrated in the drawings, the process may be
slightly modified so that only a fraction of the volume aspirated
in the syringe is delivered on each occasion to avoid contamination
between the specimens. Referring to FIG. 9, as the lugs 44 move
from position 44c, they may be made to stop by proper pinion and
rack arrangement before reaching position 44d, say half-way
therebetween thereby delivering half the quantity aspirated. The
chromatography plate is then removed and the drive bar is then
displaced to position 44d for discharging the remaining portion of
the samples in the syringes.
In cases where the aspirating trajectory 44a-44b equals the
delivering trajectory 44c-44d, an additional groove 86 (see FIG. 9)
may be cut into the inner groove 46i so that when the drive bar is
pushed fully into this groove 86 the syringe is emptied
completely.
Heating means 88 (FIG. 2) may be provided to accelerate the drying
process of the sample on the chromatography plate.
The invention has provided a tremendous improvement in the number
of samples which can be applied within an hour by a technician. It
has been found that with an apparatus having 23 syringes, 600
samples may be applied within an hour while the same technician can
only apply manually 25 samples per hour.
Although the invention has been described above in relation to one
specific form of the invention, it will be evident to those skilled
in the art that it may be modified and refined in various ways. For
example, for other types of samples, it may be necessary to provide
a different quantity of delivery; for so doing, plates 40 may be
replaced with other plates having differently spaced grooves or
with syringes having different barrel volume. It is therefore
wished to have it understood that the present invention is not
limited in interpretation except by the terms of the following
claims.
* * * * *