U.S. patent number 3,890,237 [Application Number 05/446,341] was granted by the patent office on 1975-06-17 for plasma separator -- cord stop type.
This patent grant is currently assigned to Becton, Dickinson and Company. Invention is credited to Joseph T. Welch.
United States Patent |
3,890,237 |
Welch |
June 17, 1975 |
Plasma separator -- cord stop type
Abstract
A blood collection and separator assembly of the type suitable
for use in centrifuging blood to separate the plasma or serum, the
light phase, from the cellular portion, the heavy phase. The
assembly includes a collection container and a piston disposed
therein for sealing off one phase from the other after centrifuging
is terminated. The piston is formed having a specific gravity
heavier than the light phase of the blood. The piston is slidably
disposed in the container with its outer surfaces in sealing
contact with the inner surface of the container and is provided
with means which, under sufficient centrifugal force, permit the
light phase of the blood to pass the piston as the piston moves
down through the light phase while retaining sealing engagement
with the inner surface of the container. Cord means interconnecting
the piston and the top closure of the container stop the piston at
a predetermined distance above the bottom of the container
whereupon the piston serves as an impervious barrier between the
two phases of the blood.
Inventors: |
Welch; Joseph T. (Wanaque,
NJ) |
Assignee: |
Becton, Dickinson and Company
(East Rutherford, NJ)
|
Family
ID: |
23772225 |
Appl.
No.: |
05/446,341 |
Filed: |
February 27, 1974 |
Current U.S.
Class: |
210/516; 210/789;
422/918 |
Current CPC
Class: |
B01L
3/50215 (20130101) |
Current International
Class: |
B01L
3/14 (20060101); B01d 021/26 () |
Field of
Search: |
;23/258.5,259,23B,292
;128/214R,272,2F ;210/83,84,131,359,514-518,DIG.23,DIG.24
;233/1A,1R,26 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Spear, Jr.; Frank A.
Assistant Examiner: Mukai; Robert G.
Attorney, Agent or Firm: Kane, Dalsimer, Kane, Sullivan and
Kurucz
Claims
Having described the invention what is claimed is:
1. A separator assembly, capable of separating blood into its
component phases of plasma or serum vs cellular portion,
comprising:
a. a container having first and second open ends, and adapted to
receive blood for subsequent separation into a light phase and a
heavy phase;
b. first and second closures sealing both ends of the container,
the first of said closures being formed from a self-sealing
elastomeric material which is penetrable by a cannula through which
blood to be separated is conducted into the container;
c. a piston having a specific gravity relatively greater than the
light phase of the blood and slidably mounted in the container,
said piston having means on an outer surface in sealing engagement
with an inner surface of the container, said sealing means being
constructed and arranged to maintain said piston at a first
position adjacent the second of said closures when subjected to a
first centrifugal force and to disengage when subjected to a second
greater centrifugal force so that when said container is subjected
to said first centrifugal force the blood separates into its light
phase and heavy phase but the piston stays in said first position
and subsequently when said second greater centrifugal force is used
the sealing means automatically disengages, the light phase thereby
passing around the sealing means enabling the piston to move down
through the light phase; and
d. stop means comprising a cord of a predetermined length extending
from said second closure to the piston whereby the piston when
moving through the light phase will stop a predetermined distance
from said first end of the container.
2. The separator of claim 1 wherein the sealing means associated
with the said piston comprises flexible sealing fins on the outer
surface of the piston in sealing engagement with an inner surface
of the container which fins are slanted in an upward direction and
are adapted when centrifugal force is applied to the assembly to
permit the light phase to pass around the periphery of the piston
when the piston moves down through the light phase.
Description
BACKGROUND OF THE INVENTION
It is known to separate blood into its component parts by
centrifugation, for example, the assembly disclosed in U.S. Pat.
No. 2,460,641. However, this particular assembly does not employ a
means for sealing the separated plasma or serum phase from the
cellular phase.
It is also known to provide assemblies for manually separating the
plasma or serum phase from the cellular phase, for example, as
disclosed in U.S. Pat. Nos. 3,586,064; 3,661,265; 3,355,098;
3,481,477; 3,512,940; and 3,693,804. In all of these devices the
serum is collected in a blood collection container and means are
provided for separating the plasma or serum phase from the cellular
phase employing filters, valves, transfer tubes or the like.
It is also known to provide assemblies for the sealed separation of
blood in which a piston is actuated by centrifugal force such as is
disclosed in U.S. Pat. Nos. 3,508,653 and 3,779,383. These devices
use either a resilient piston or valve means associated with the
piston to effect a sealed separation after centrifugation.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide a serum/plasma
separator assembly including a collection container and a piston
slidably disposed in the container, which piston has means
permitting, under sufficient centrifugal force, the light phase of
the blood to pass the piston as the piston moves through the light
phase, means to stop the piston at a predetermined distance above
the bottom of the container, and means to seal the piston in the
container at the plasma/serum-cellular interface.
It is another object of the invention to provide a serum/plasma
separator assembly which is economical to manufacture and can be
used in conjunction with standard blood collecting equipment.
DESCRIPTION OF THE DRAWINGS
For a better understanding of the invention, references are made to
the drawings which illustrate the preferred embodiment of the
invention herein.
FIG. 1 is a sectional, elevational view showing the plasma/serum
separator assembly of the present invention and also illustrating a
pointed cannula penetrating one of the stoppered ends of the
container through which blood is introduced into the container
prior to blood separation into its light and heavy phases.
FIG. 2 is a sectional, elevational view similar to the view of FIG.
1, showing the piston stopped at a predetermined distance above the
bottom of the container determined by the length of the cord
connecting the top closure to the piston, said piston sealing the
container at the plasma/serum-cellular interface.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
For a better understanding of the invention herein a description of
the drawings of the illustrative embodiments is had with particular
reference to FIGS. 1 and 2.
In FIG. 1 the separator assembly 10 comprises a tubular member or
container 11 which is sealed at its open ends by closure members 12
and 13. Such tubular member or container is preferably formed of
glass but any other suitable material may be employed. Closure
members 12 and 13 are preferably made of rubber. Closure 12 is
capable of being penetrated by a cannula 14, so that blood can be
transferred from a blood source into the container under aseptic
conditions. The closures 12 and 13 are preferably made of
elastomeric material and should be self-sealing so that when the
cannula 14 is removed from the closure 12 there will be no loss of
blood.
Disposed in container 11 is piston 15 which is preferably made of
an elastomeric material and is made to provide an interference fit
within container 11. The piston is formed of material having a
specific gravity heavier than the liquid phase of blood. After
cannula 14 is removed, the assembly is centrifuged first at a low
speed and later at a high speed. Piston 15 is made with sufficient
friction to stay up during low speed spin but move downwardly
during the high speed spin, down through the plasma/serum phase,
from the initial starting position illustrated in FIG. 1 to the
terminal position after the separation of the light phase from the
heavy phase as shown in FIG. 2.
The elastomeric (preferably rubber) portion of piston 15 has one or
more very flexible axially spaced sealing fins 15a encircling the
periphery of the piston. These fins contact the inner wall surface
11a of the container 11 in sealing engagement. Such fins preferably
extend in a diagonally upward direction so that when centrifugal
force is applied to the assembly the fins will yield and permit the
liquid phase to pass up around the outer periphery of the piston as
the piston descends. When the piston has reached its stopping point
just above the interface these fins will reestablish a seal
relative to the inner wall 11a of the container 11.
One end of cord 16 is secured to the piston 15 and at the other end
to the closure member 13. Such a cord can be a monofilament of
plastic or other suitable material. The closure member 13 is
provided with a recess 13a so that the cord can be coiled within
the recess. The cord is of a predetermined length to stop the
piston 15 at a predetermined distance from the bottom of the
container. Such a cord is made to position the piston slightly
above the serum/plasma-cellular interface.
As illustrated in FIG. 2, piston 15 has completed its travel within
container 11 and is stopped from further downward movement in
container 11 by the cord stop means and the piston fins 15a are
resealed relative to the tube 11. Also a portion of the light phase
remains above the separated heavy phase and is not utilized as part
of the separated light phase.
When operating the separator assembly of the invention herein it is
preferred that the assembly be evacuated so that when cannula 14
penetrates closure 12 blood will fill container 11. It is also
important when filling the assembly 10 that blood be introduced
into container 11 through the stopper 12 mounted on the bottom of
the container to obviate the possibility of having blood cells
trapped between the piston 15 and stopper 13 which will later
separate to form the chamber where the light phase will be
collected. Blood cells above piston 15 would contaminate the
serum/plasma.
When the centrifuging is terminated, the separated blood sample is
ready for use. As desired, the serum or plasma can be taken from
one end and/or the concentrated red cells can be taken from the
other end.
While variations of the invention herein may be had, the objectives
of the invention have been illustrated and described.
* * * * *