U.S. patent number 3,886,928 [Application Number 05/297,219] was granted by the patent office on 1975-06-03 for device for the extraction of blood.
Invention is credited to Walter Sarstedt.
United States Patent |
3,886,928 |
Sarstedt |
June 3, 1975 |
Device for the extraction of blood
Abstract
The invention relates to a device for the extraction of blood
comprising a sleeve and a piston which can be fabricated simply and
economically and whereby the sleeve of which is suited for
subsequent centrifugalizing in the same way as a conventional
centrifuge tube. This invention is characterized in that a tube
closed at one end is used as a sleeve and that a punctured piston
is used having a tubular piston rod adjacent thereto, whereby at
the free end of said piston rod projecting from the tube there are
disposed lateral handles and the connecting socket for applying a
tubule.
Inventors: |
Sarstedt; Walter (5223
Numbrecht, Rommelsdorf, DT) |
Family
ID: |
46679342 |
Appl.
No.: |
05/297,219 |
Filed: |
October 13, 1972 |
Foreign Application Priority Data
|
|
|
|
|
Oct 13, 1971 [DT] |
|
|
2150884 |
Sep 16, 1972 [DT] |
|
|
2245584 |
|
Current U.S.
Class: |
600/578; 422/513;
494/16; 604/231; 494/38 |
Current CPC
Class: |
A61B
5/150755 (20130101); A61B 5/150519 (20130101); A61B
10/00 (20130101); A61B 5/150244 (20130101); A61B
5/150236 (20130101); G01N 33/491 (20130101); A61B
5/153 (20130101); A61B 5/150259 (20130101); A61B
5/150389 (20130101); A61B 5/150099 (20130101); A61B
5/15003 (20130101) |
Current International
Class: |
A61B
5/15 (20060101); G01N 33/49 (20060101); A61m
001/00 () |
Field of
Search: |
;128/2F,2G,214B,218P,218PA,218M,218D,218DA,DIG.5,DIG.22,235,237,238
;23/258.5 ;210/83,DIG.15 ;141/236,8,363 ;73/425.4,423A |
References Cited
[Referenced By]
U.S. Patent Documents
Foreign Patent Documents
|
|
|
|
|
|
|
888,863 |
|
Dec 1971 |
|
CA |
|
1,179,487 |
|
May 1967 |
|
GB |
|
87,197 |
|
Jan 1965 |
|
FR |
|
Primary Examiner: Gaudet; Richard A.
Assistant Examiner: Recla; Henry J.
Attorney, Agent or Firm: Diller, Brown, Ramik &
Wight
Claims
What is claimed is:
1. A device for the extraction of blood by means of a suction
effect comprising a tube having a closed end and an open end, a
one-piece piston assembly cooperatively telescoped within said
tube, said piston assembly comprising a piston having an aperture
therethrough, a hollow piston rod extending from said piston in
communication with the aperture therein and extending out through
said tube open end, said piston being in sealed engagement with the
interior of said tube and said piston rod having axially elongated
generally radially extending wings guidedly engaging the interior
of said tube, said piston rod having a free end in the form of
means for the reception of a needle in sealed engagement, a
transversely disposed handle on said piston rod adjacent said free
end for withdrawing said piston assembly from said tube to produce
a suction within said piston rod, said hollow piston rod has a
tapered bore, said bore decreasing in cross section from said
piston towards said free end whereby a maximum suction is effected
at said piston rod free end.
2. A device for the extraction of blood by means of a suction
effect comprising a tube having a closed end and an open end, a
one-piece piston assembly cooperatively telescoped within said
tube, said piston assembly comprising a piston having an aperture
therethrough, a hollow piston rod extending from said piston in
communication with the aperture therein and extending out through
said tube open end, said piston being in sealed engagement with the
interior of said tube and said piston rod having axially elongated
generally radially extending wings guidedly engaging the interior
of said tube, said piston rod having a free end in the form of
means for the reception of a needle in sealed engagement, a
transversely disposed handle on said piston rod adjacent said free
end for withdrawing said piston assembly from said tube to produce
a suction within said piston rod, said hollow piston rod is tapered
from said piston to said free end, and said piston rod has a
similarly tapered bore whereby a maximum strength to weight ratio
is obtained together with a maximum suction being effected at said
piston rod free end.
3. A device for the extraction of blood by means of a suction
effect comprising a tube having a closed end and an open end, a
one-piece piston assembly cooperatively telescoped within said
tube, said piston assembly comprising a piston having an aperture
therethrough, a hollow piston road extending from said piston in
communication with the aperture therein and extending out through
said tube open end, said piston being in sealed engagement with the
interior of said tube and said piston rod having axially elongated
generally radially extending wings guidedly engaging the interior
of said tube, said piston rod having a free end in the form of
means for the reception of a needle in sealed engagement, a
transversely disposed handle on said piston rod adjacent said free
end for withdrawing said piston assembly from said tube to produce
a suction within said piston rod, a plug of resilient plastic, said
plug having a knob and an adapter portion axially adjacent thereto,
said adapter portion having a peripheral surface sealingly
engageable with said tube and a conical axial bore receiving said
piston rod free end, said axial bore being closed at its inner
end.
4. Device according to claim 3, characterized in that at least one
annular bead projects radially outwardly from the peripheral
surface of the adapter portion.
5. Device according to claim 3, characterized in that the conical
bore is defined by a hat-shaped inverted portion extending from the
free end of the adapter portion axially inwardly.
6. A device for the extraction of blood by means of a suction
effect comprising a tube having a closed end and an open end, a
one-piece piston assembly cooperatively telescoped within said
tube, said piston assembly comprising a piston having an aperture
therethrough, a hollow piston rod extending from said piston in
communication with the aperture therein and extending out through
said tube open end, said piston being in sealed engagement with the
interior of said tube and said piston rod having axially elongated
generally radially extending wings guidedly engaging the interior
of said tube, said piston rod having a free end in the form of
means for the reception of a needle in sealed engagement, a
transversely disposed handle on said piston rod adjacent said free
end for withdrawing said piston assembly from said tube to produce
a suction within said piston rod, a granulate in said tube between
said piston and said tube closed end, said granulate being a
chemically inert substance for functioning as a separating agent
between two blood fractions, and including surface proximate layers
with embedded small glass particles, preferably fibres, partially
projecting outwardly from the surface thereof.
7. A device for the extraction of blood by means of a suction
effect comprising a tube having a closed end and an open end, a
one-piece piston assembly cooperatively telescoped within said
tube, said piston assembly comprising a piston having an aperture
therethrough, a hollow piston rod extending from said piston in
communication with the aperture therein and extending out through
said tube open end, said piston being in sealed engagement with the
interior of said tube and said piston rod having axially elongated
generally radially extending wings guidedly engaging the interior
of said tube, said piston rod having a free end in the form of
means for the reception of a needle in sealed engagement, a
transversely disposed handle on said piston rod adjacent, said free
end for withdrawing said piston assembly from said tube to produce
a suction within said piston rod, a granulate in said tube between
said piston and said tube closed end, said granulate being a
chemically inert substance for functioning as a separating agent
between two blood fractions, and the particles of said granulate
having a coating of a substance for retarding blood
coagulation.
8. A device for the extraction of blood by means of a suction
effect, said device comprising a tube having a closed end and an
open end, a one piece integrally molded piston assembly
cooperatively telescoped within said tube, said piston assembly
comprising an inverted cup shaped piston, a tubular rod, axially
extending guides, and handle means; said cup shaped piston having a
cylindrical portion in sliding sealed engagement with said tube and
an end wall, said tubular piston rod being carried by said piston
end wall in eccentric relation to said piston and tube and
projecting out of said tube, said piston rod having a free end,
means only at said free end for the mounting of a needle thereon,
said axially extending guides projecting generally radially from
said piston rod and guidedly engaging said tube, at least two of
said buides being of appreciably different radial dimensions due to
the eccentricity of said piston rod relative to said tube, a bore
in said piston end wall in communication with the interior of said
piston rod, and said handle means on said piston rod being adjacent
said free end.
9. A device for the extraction of blood by means of a suction
effect, said device comprising a tube having a closed end and an
open end, a piston assembly cooperatively telescoped within said
tube, said piston assembly comprising an inverted cup shaped piston
having a cylindrical portion in sliding sealed engagement with said
tube and an end wall, a tubular piston rod carried by said piston
end wall in eccentric relation to said piston and tube and
projecting out of said tube, said piston rod having a free end
portion defining means for the mounting of a needle axially
extending guides projecting generally radially from said piston rod
and guidedly engaging said tube, a bore in said piston end wall in
communication with the interior of said piston rod, handle means on
said piston rod adjacent said free end, said piston rod is
conically reducing in cross section from said piston toward said
free end whereby at least two of said guides taper in opposite
directions relative to each other and to the length of said piston
rod.
Description
This invention relates to a device for the extraction of blood,
comprising a sleeve, a piston guided shiftable and liquid-tight
therein and a socket communicating with the interior for applying a
tubule.
Such devices are known in the form of syringes which are also used
to inject liquid. In recent times such syringes have been formed in
such a way, in particular in case they are used for extracting
blood, that they are used only once and then are disposed off.
These syringes have already been sterilized by the manufacturer and
have been packaged sterile so that for the doctor the activity of
sterilizing is eliminated. Also it is rendered impossible when
using such syringes once that syringes already used are used a
further time without a subsequent cleaning and sterilization or
after an incomplete sterilization and result in an infection of the
second patient.
Provided blood is extracted from a patient with such syringes and
is to be centrifugalized, this blood is subsequently injected into
a centrifuge tube. This tube is closed by a plug by the doctor or
in the hospital and is transferred to the test laboratory, while
the syringe is disposed off.
In order to avoid the filling over required in this regard and to
save the special centrifuge tube, it has been proposed to use the
sleeve of the syringe as a centrifuge tube, by closing the socket
disposed at the bottom of this sleeve after removing the tubule
with a special closure cap.
By the fact that the re-injection into a special centrifuge tube is
dispensed with, a lesser haemolysis results and confusion and
defilement possibilities are eliminated. Also the centrifuge tubes
are rendered superfluous.
The disadvantage of this single-use syringe for the extraction of
blood, however, is that the bottom of the tube or of the sleeve
greatly stresses in the centrifuge is closed by an applied closure
cap which can happen to detach or become untight in an adverse
instance. In such a case the blood perhaps even containing germs
would seep out of the bottom of the tube and would at least pollute
the centrifuge, if not perhaps even the environment and depending
on circumstances infect it.
Also there is the risk with this conventional single-use syringe
that the socket after removing the tubule and prior to applying the
closure cap is polluted during the manipulation and possibly is
infected. This could in the adverse case detrimentally affect the
tests.
An object of this invention is to eliminate the disadvantages of
the conventional devices and to provide a device for the extraction
of blood which is simple and economical to make and the sleeve of
which is suited for a subsequent centrifugalizing in the same way
as the conventional centrifuge tube, after removing the piston with
the tubule.
This invention resides in the measure that as a sleeve a tube
closed at one side and as a piston a punctured piston with an
adjacent tubular piston rod is used at the free end of which
projecting from the tube there are lateral handles and the socket
for applying a tubule.
Prior to use, the piston is pushed completely into the tube, is
sterilized and is packed sterile. After puncturing the vein by the
tubule the tubule and thus also the tubular piston rod and the
piston are held fixed by means of the handles, while the tube is
drawn away from the handle. Thereby in the interior of the tube a
depression results which causes the entry of blood through the
tubule via the tubular piston rod into the bottom area of the
tube.
After extracting a sufficient quantity, the tubule is removed from
the vein. The piston is drawn out of the tube, and the tube is
closed by a plug before it is placed into the centrifuge. Prior to
pulling the piston out of the tube, the tubule can be removed, or
it can also be left on the socket. The blood contained in the
tubular piston rod is not lost for the test, since it when pulling
the piston out of the tube, is sucked into the latter.
Since a subsequent refilling of the extracted blood into a
centrifuge tube is omitted, the haemolysis is maintained to a
minimum possible rating.
The use of additional centrifuge tubes next to the sleeve of the
device is dispensed with or this sleeve is defined by the normal
centrifuge tube.
In certain applications and in particular for devices according to
the main patent in which for the extraction of larger amounts of
blood the tube accomodating the piston has a larger diameter, it
has proven to be desirable to not arrange the connecting socket for
applying an injection needle concentric relative to the tube, i.e.
in a greater spacing from the circumference thereof, but eccentric,
namely as closely as possible to the circumference of the tube. For
this affords substantial advantages upon introducing the injection
needle in a vein and the subsequent extraction, for manipulation,
because now the tube can be held parallel to the surface of the arm
or the like along its entire length and a disturbed holding and
thus the risk of an injury of the opposing vein wall with an
inclined holding is not provided or is hardly provided anymore.
While now in the conventional blood extraction devices in which the
connecting socket is arranged at the end of the tube, i.e., the so
called syringe cylinder, and the piston is not bored through, such
an eccentric embodiment can be easily provided because of the
arrangement of the injection syringes in several parts, this is not
possible because of certain difficulties in manufacture for the
device according to the main patent in which the piston, the piston
rod, the handle and the connecting socket for the injection needle
are injection molded in one piece of plastic.
The device for extracting blood, therefore, preferably is furthered
in such a way that the piston includes a thin circular disk
connected to the guide veins and a thin circular cylindrical collar
projecting from the circumference thereof in axial direction.
Even in case of a deviation of the circumference of the circular
disk from the desired circular shape the collar projecting from it
always will attempt to have its circular cylindrical shape. The
collar thus will in all events sealingly engage the inner surface
of the tube, in particular with its free edge, even in case of
deviations of the disk from the circular shape. By this
configuration of the piston the difficulties are eliminated which
result in the manufacture of the piston with the piston rod in one
piece.
Here also guide wings are provided which extend outwardly from the
tubular piston rod and take over the guiding of the piston rod in
the tube.
Preferably the bottom of the sleeve can be filled with a substance
suppressing a blood coagulation. This substance may also be applied
to the inner wall of the tubular piston rod.
It is preferably provided for in this regard that the tubular
piston rod conically converges from the opening in the piston
toward the socket. Thereby, a gradual transition from the large
cross section of the opening in the piston to the small cross
section of the tubule or vice versa is provided for. By this flow
enhancing configuration the risk of an haemolysis is again
reduced.
In order to accomplish a satisfactory guiding of the piston and the
piston rod in the tube and in order to prevent a cocking, it is
furthermore proposed to provide at least three guide wings
extending radially outwardly from the piston rod and extending in
the longitudinal direction thereof, said guide wings with their
outer edges extending parallel to one another and to the
longitudinal axis of the piston rod and defining an enveloping
cylinder which corresponds to the inner peripheral wall of the
tube. The piston can now be formed as a simple circular disc, and
an excellent guiding and sealing effect is accomplished in
particular in case the piston, the piston rod and the guide wings
are made of a resilient plastic. The tube can be made in a manner
known per se either of glass or of a plastic.
According to this invention a plug of resilient material is
associated to the tube, said plug having a knob and an adapter
portion adjacent thereto which has a peripheral surface sealingly
fitting into the cylinder and a tapered axial bore adapted to the
tapered socket of the plunger, said axial bore being closed at its
inner reduced end. This plug is formed in such a way that it
greatly facilitates the manipulation of the device. Devices of this
type are supplied with an inserted plunger and packed sterile. In
order to prevent a defilement of the bore of the plunger prior to
applying a sterile injection needle, a cap can be applied to this
socket. Also a plug is required after the exctraction of blood for
closing the tube, after the plunger together with the injection
needle has been removed out of the tube. The plug of this invention
now combines this cap and the closure plug in a single member. The
plug thus with its tapered axial bore initially plugs the socket at
the free end of the plunger and later after the removal of the
plunger from the tube plugs this tube with its larger adapter
portion.
In order to improve the sealing effect, the peripheral surface of
the adapter portion is formed slightly tapered. Preferably an
annular bead projects radially outwardly from the peripheral
surface of the adapter portion which likewise improves the sealing
effect. Alternatingly, a plurality of annular beads may be provided
also.
The tapered bore at one end of the plug is preferably defined by a
hat-shaped recess extending axially inwardly. Then the plug
otherwise can be formed generally hollow so that its weight and
thus also its price can be kept low.
Depending on whether the blood extracted from a patient is to
coagulate and then its constituents are to be examined further or
whether the blood is supposed to be centrifugalized without
coagulating, it is filled in present day practice from the
extraction device into a centrifuge tube or some other container.
With the device of this invention the centrifugalizing or also the
coagulation process can now be effected in the same tube in which
the socket is disposed with the applied injection needle after the
removal of this socket.
In order to separate as satisfactorily as possible the two
fractions of the blood forming upon centrifugalizing or upon
coagulating, the device according to this invention has been
developed further in such a way that a chemically inert granulate
is added to the tube as a separating agent. This granulate thus is
contained in the device packaged sterile comprising the tube and
the socket pushed thereinto.
The separating agent assumes a position between the two fractions
in the later separation of the blood, be it during coagulation, be
it also during centrifugalizing, thus facilitating the examination
of one of these two parts alone. The advantage of the combination
of the tube with the socket and the separating agent is that
thereby a complete device is provided ready for use and that
thereby the servicing is extremely facilitated, in particular a
refilling is rendered superfluous.
Prior to using the device, it is preferably sterilized inclusive
the separating agent and packaged sterile.
When drawing the blood quantity in, it directly encounters the
separating agent disposed in the bottom area of the tube.
The granulate filled into the tube preferably comprises a plastic
the specific weight of which is between that of the blood cake and
that of the serum, i.e. between the specific weights of the two
fractions. As a result thereof the separating agent properly
assumes a position between the two constituents and separates them
from one another.
Furthermore it is proposed to introduce into the tube of the device
for the extraction of blood a granulate of a chemically inert
substance, the grains of this granulate being coated with a
chemical substance at their surface which either accelerates the
blood coagulation or, in case this is desired, retards the blood
coagulation. Thus, by using various granulates either immediately
an accelerated coagulation can be caused so that the examination of
the two fractions can be effected earlier than up to now. But on
the other hand any blood coagulation can be prevented from the
start on by applying a coagulation retarding substance to the
surface of the granulate grains so that the blood extracted
immediately contacts this coagulation retarding substance.
By the fact that the said chemical substance is provided in a thin
layer on a relatively large surface, namely the overall surface of
all grains of the granulate, this substance has a particularly
quick effect. Substances which retard or accelerate the blood
coagulation, of course, have been known already and have also
already been used for this purpose, but not as a coating on a
granulate as a vehicle which is directly filled into the device for
the extraction of blood. This granulate can also at the same time
be provided as a separating agent as mentioned hereinbefore.
According to this invention it is furthermore proposed to not coat
the surface of the granulate grains with a chemical substance in a
thin layer in order to thereby influence the coagulation of the
blood, rather it is proposed, for accelerating the coagulation of
the blood, that in the surface proximate layers of the granulate
grains small glass particles, preferably glass fibres, are
embedded. These glass particles projecting from the surface of the
granulate grains have a purely physical effect, by influencing the
surface tension at the interface, on the blood coagulation. It has
been observed that the blood coagulation proceeds slower in vessels
of thermoplastic materials, in particular of polyethylene and
polypropylene, than in vessels of glass. In some instances such a
delay in the blood coagulation is utterly undesired. Up to now then
the use of plastic vessels instead of glass vessels had to be
dispensed with, although plastic vessels have many other
advantages. The glass particles introduced in the surface of the
granulate grains according to this invention now bring about
substantially accelerated blood coagulations. By a metered addition
of these glass particles or the granulate grains provided with such
glass particles the blood coagulation can now be accelerated in any
desired way, or the slowing down of the blood coagulation can
merely be compensated which results when using tubes of plastic, so
that thereby blood coagulation ratings are obtained like they
result in glass tubes.
Provisions are thus available with these granulate grains including
embedded glass particles to arbitrarily accelerate the blood
coagulation without having to use chemical substances which perhaps
might interfere specific tests.
It is essential for the effect of the granulate proposed according
to this invention that the glass particles project from the surface
of the granulate grain carrying them without themselves being
encased and covered by the substance of the granulate.
A plastic granulate of a conventional grain size is particularly
suited for this purpose like it is used for charging plastic
processing machines, for instance extruders. Basically all plastics
are suited for this provided they are not objectionable for
chemical reasons and interfere the blood examination. Fine glass
particles, for instance short fibres of glass, are then to be
included in the individual grains of the granulate. Such plastic
granulates or intermediate products of plastic which are enforced
by glass fibres or glass particles are known already. They are
suited as an aid for the examination of blood in the sense of the
invention only in the event the glass particles, for instance the
ends of the glass fibres, project outwardly from the surface of the
plastic particles and thus become physically effective. It is to be
observed when producing such granulates that the glass particles or
at least some of them project outwardly from the surface.
This invention is explained hereinafter in embodiments in referring
to the drawing. Therein:
FIG. 1 is a longitudinal sectional view of the device of this
invention;
FIG. 2 is an enlarged cross sectional view along the line II-II in
FIG. 1;
FIG. 3 is a sectional view of a modified device with an eccentric
duct, in a section along line III--III of FIG. 4;
FIG. 4 is a sectional view along line IV-IV of FIG 3;
FIG. 5 is a device similar to the device illustrated in FIG. 1, in
a side elevation of view, with applied plug;
FIG. 6 is a sectional view of the plug in FIG. 5 and of the conical
socket of the plunger;
FIG. 7 is a sectional view along line VII--VII of FIG. 6;
FIG. 8 is a sectional view of the plug as illustrated in FIG. 6,
but inserted in the end of the tube;
FIG. 9 is a simplified sectional view of a tube of the device in
the filled condition; and
FIG. 10 is a sectional view in an enlarged scale of a granulate
particle with embedded glass fibres.
The device illustrated in FIG. 1 comprises a tube 1 closed at one
side, a socket inserted therein, which includes a tubular piston
rod 2, four lateral guide wings 3 and at their lower end a
disc-shaped piston 4, two radially outwardly projecting handles 5
which are to facilitate the manipulation of the device, and a
connecting socket 7 for applying the cap 8 of an injection needle
9. The piston 4, the tubular piston rod 2, the guide wings 3, the
connecting socket 7 and the handles 5 are made in one piece and
preferably of a resilient plastic, for instance polypropylene.
The tube can be made in a conventional way of glass or also of a
plastic, for instance of polystyrene.
As will be noted from FIG. 2, the guide wings 3 closely engage the
inner peripheral wall of the tube 1 with their outer edges and
thereby guide the piston properly in the tube.
The modified embodiment illustrated in FIGS. 3 and 4 of the blood
extraction device again includes a tube 101 closed at one side, a
plunger 102 shiftable in a hermetically sealing fashion in this
tube, a tubular plunger rod 103 connected thereto and at the end
thereof a handle 109 as well as a connecting socket 110 for
applying an injection needle.
The piston comprises a thin circular disc 111 which may possibly
slightly deviate from the circular shape in manufacture, and a thin
circular cylindrical collar 112 projecting from the periphery
thereof in axial direction, said collar taking over the sealing
function in relationship to the tube 101.
The bore 104 through the circular disc 111 and the plunger rod 103
connected thereto are arranged concentrical in relationship to the
central axis of the tube 101. Accordingly the connecting socket 110
for applying an injection needle also is disposed relatively closed
to the circumference of the tube 101.
Guide wings 105, 106, 107 and 108 extend from the plunger rod 103
outwardly to the inner wall of the tube. These veins have different
lengths because of the eccentric position of the plunger rod.
In the device illustrated in FIG. 5 a plug 10 is disposed on the
connecting socket 7a and has a knob 12 which at the outside is
provided with corrugations or the like. An adapter portion 11 is
provided axially adjacent to the knob and has a radially projecting
narrow annular bead 14.
Otherwise the device according to FIG. 5 again comprises a tube 1a,
a tubular piston rod 2a with guide wings 3a and handles 5a as well
as a disc-shaped piston 4a at the free end of the piston rod 2a.
The connecting socket 7a serves to apply the cap of an injection
needle when preparing the device for use.
It will be noted from the sectional view of FIG. 6 that the plug 10
is formed generally hollow and has a conical inverted portion 15
setting out from the bottom of the adapter portion 11. This
inverted portion has an inner conical peripheral surface which is
adapted to the conical outer peripheral surface of the socket 7a of
the plunger. With the plug applied thus the longitudinal bore 6a of
the plunger and of the socket 7a is covered and closed by the
hat-shaped inverted portion 15.
As will be noted from FIG. 6 in conjunction with FIG. 7, the
comparatively thin walls of the knob 12 of the plug are supported
by four radial walls 13 which at their lower or inner end, as will
be noted from FIG. 6, are connected to the closed end of the
inverted portion 15 and thereby are also supported in relationship
to this inverted portion. Thereby a great increasing of the
strength of the plug is accomplished even with a thinwalled
configuration. On the other hand the thin-walled configuration
facilitates the sealing in the vicinity of the annular bead 14 of
the adapter portion 11.
A granulate 16 of plastic, acting as a separating agent, is filled
into the tube 1a.
The sectional view of FIG. 8 shows how the adapter portion 11 of
the plug is inserted in the upper opening of the tube 1a and how
the annular bead 14 in doing so causes a reliable sealing effect.
It will be noted that the adapter portion 11 is radially offset at
the junction to the knob 12 relative thereto. The shoulder defined
thereby serves as an abutment when inserting the plug in the tube
1a.
In FIG. 9 finally the tube 1b of a device of this invention has
been illustrated filled with a blood sample. The figure shows the
blood sample after centrifugalizing. In the lower portion of the
tube the sedimented blood-corpuscles 19 are disposed, thereabove
the separating layer 18, comprising the granulate acting as a
separating agent, which at its surface was coated with a substance
retarding the blood coagulation, thereabove the liquid plasma
17.
The same picture virtually results when the blood is caused to
coagulate and the blood is then centrifugalized. Then the
coagulated blood cake is disposed at the bottom of the tube,
thereabove the separating agent layer and thereabove the liquid
serum. In this case preferably a separating agent is used with a
chemical substance for accelerating the blood coagulation or with
embedded glass particles which have the same effect. Various
chemical substances are known which chemically have an effect on
the blood coagulation process and accelerate it.
But it also known that the blood coagulation can be influenced by
physical means. For instance blood coagulates slower in vessels of
thermoplastic materials than in vessels of glass, which is to be
explained by the different surface tension and the physical
conditions at the interface between the blood and the container
wall.
In furtherance of the invention it is not proposed that the
substance accelerating the blood coagulation and disposed on the
top surface of the granulate grains is glass, namely in the form of
fine particles, in particular fibres, which are embedded in the
outer layer of each granulate grain. Principally all plastics are
suited for such a granulate, provided they do not interfere with
the blood examination for chemical reasons. The glass fibres are to
project outwardly from the surface of the plastic parts, in order
to become physically effective therewith. In FIG. 10 such a
granulate particle 20 has been illustrated to an enlarged scale,
namely in cross section to the left side of the section line A--A
and in a elevation of view at the right hand of this line. The
glass fibres 21 embedded irregularly in the granulate particles 20
will be noted which project from the surface of the granulate
particle 20.
* * * * *